LOCUS       DAFFSV010000001        67289 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFFSV010000001 DAFFSV010000000
VERSION     DAFFSV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.001"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            41..922
                     /locus_tag="LR110_000001"
     CDS             41..922
                     /locus_tag="LR110_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4821009.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            935..2545
                     /locus_tag="LR110_000002"
     CDS             935..2545
                     /locus_tag="LR110_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBL4821010.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2622..3281)
                     /locus_tag="LR110_000003"
     CDS             complement(2622..3281)
                     /locus_tag="LR110_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL4821011.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3564..4229)
                     /locus_tag="LR110_000004"
     CDS             complement(3564..4229)
                     /locus_tag="LR110_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBL4821012.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="LR110_000005"
     CDS             complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="LR110_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBL4821013.1"
                     /translation="MKLTVQEIQEWLAQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5009..6019)
                     /gene="dhaK"
                     /locus_tag="LR110_000006"
     CDS             complement(5009..6019)
                     /gene="dhaK"
                     /locus_tag="LR110_000006"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBL4821014.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5994..6440)
                     /gene="dhaM"
                     /locus_tag="LR110_000007"
     CDS             complement(5994..6440)
                     /gene="dhaM"
                     /locus_tag="LR110_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBL4821015.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6543..6863
                     /locus_tag="LR110_000008"
     CDS             6543..6863
                     /locus_tag="LR110_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBL4821016.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6975..7844)
                     /locus_tag="LR110_000009"
     CDS             complement(6975..7844)
                     /locus_tag="LR110_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL4821017.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7940..8320)
                     /locus_tag="LR110_000010"
                     /pseudo
     CDS             complement(7940..8320)
                     /locus_tag="LR110_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     gene            complement(8284..8631)
                     /locus_tag="LR110_000011"
     CDS             complement(8284..8631)
                     /locus_tag="LR110_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821018.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKLEERSSSLIKH"
     gene            complement(8806..10941)
                     /locus_tag="LR110_000012"
     CDS             complement(8806..10941)
                     /locus_tag="LR110_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4821019.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(11024..12679)
                     /locus_tag="LR110_000013"
     CDS             complement(11024..12679)
                     /locus_tag="LR110_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL4821020.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(12681..12995)
                     /locus_tag="LR110_000014"
     CDS             complement(12681..12995)
                     /locus_tag="LR110_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL4821021.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            13165..13425
                     /locus_tag="LR110_000015"
     CDS             13165..13425
                     /locus_tag="LR110_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBL4821022.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            13439..13753
                     /locus_tag="LR110_000016"
     CDS             13439..13753
                     /locus_tag="LR110_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBL4821023.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(14483..15037)
                     /locus_tag="LR110_000017"
     CDS             complement(14483..15037)
                     /locus_tag="LR110_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBL4821024.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(15163..17091)
                     /locus_tag="LR110_000018"
     CDS             complement(15163..17091)
                     /locus_tag="LR110_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL4821025.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(17397..18113)
                     /locus_tag="LR110_000019"
     CDS             complement(17397..18113)
                     /locus_tag="LR110_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL4821026.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(18114..19937)
                     /gene="glpO"
                     /locus_tag="LR110_000020"
     CDS             complement(18114..19937)
                     /gene="glpO"
                     /locus_tag="LR110_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBL4821027.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(19951..21447)
                     /gene="glpK"
                     /locus_tag="LR110_000021"
     CDS             complement(19951..21447)
                     /gene="glpK"
                     /locus_tag="LR110_000021"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBL4821028.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(21583..23043)
                     /locus_tag="LR110_000022"
     CDS             complement(21583..23043)
                     /locus_tag="LR110_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4821029.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(23033..24328)
                     /locus_tag="LR110_000023"
     CDS             complement(23033..24328)
                     /locus_tag="LR110_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL4821030.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(24660..25337)
                     /locus_tag="LR110_000024"
     CDS             complement(24660..25337)
                     /locus_tag="LR110_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBL4821031.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(25367..26458)
                     /locus_tag="LR110_000025"
     CDS             complement(25367..26458)
                     /locus_tag="LR110_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBL4821032.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(26559..26993)
                     /locus_tag="LR110_000026"
     CDS             complement(26559..26993)
                     /locus_tag="LR110_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4821033.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(27577..29034)
                     /gene="ascB"
                     /locus_tag="LR110_000027"
     CDS             complement(27577..29034)
                     /gene="ascB"
                     /locus_tag="LR110_000027"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL4821034.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(29031..30926)
                     /locus_tag="LR110_000028"
     CDS             complement(29031..30926)
                     /locus_tag="LR110_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL4821035.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(30984..32759)
                     /locus_tag="LR110_000029"
     CDS             complement(30984..32759)
                     /locus_tag="LR110_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL4821036.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(32809..34305)
                     /locus_tag="LR110_000030"
     CDS             complement(32809..34305)
                     /locus_tag="LR110_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL4821037.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(34499..36121)
                     /locus_tag="LR110_000031"
     CDS             complement(34499..36121)
                     /locus_tag="LR110_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL4821038.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(36570..37883)
                     /locus_tag="LR110_000032"
     CDS             complement(36570..37883)
                     /locus_tag="LR110_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL4821039.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(38128..38352)
                     /locus_tag="LR110_000033"
     CDS             complement(38128..38352)
                     /locus_tag="LR110_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821040.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(38372..38794)
                     /locus_tag="LR110_000034"
     CDS             complement(38372..38794)
                     /locus_tag="LR110_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBL4821041.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(38937..39647)
                     /locus_tag="LR110_000035"
     CDS             complement(38937..39647)
                     /locus_tag="LR110_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821042.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(39665..40162)
                     /locus_tag="LR110_000036"
     CDS             complement(39665..40162)
                     /locus_tag="LR110_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4821043.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(40199..40651)
                     /locus_tag="LR110_000037"
     CDS             complement(40199..40651)
                     /locus_tag="LR110_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4821044.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(40786..41391)
                     /locus_tag="LR110_000038"
     CDS             complement(40786..41391)
                     /locus_tag="LR110_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL4821045.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(41841..42461)
                     /locus_tag="LR110_000039"
     CDS             complement(41841..42461)
                     /locus_tag="LR110_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL4821046.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(42463..43332)
                     /locus_tag="LR110_000040"
     CDS             complement(42463..43332)
                     /locus_tag="LR110_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBL4821047.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(43355..43762)
                     /locus_tag="LR110_000041"
     CDS             complement(43355..43762)
                     /locus_tag="LR110_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL4821048.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(43781..45070)
                     /locus_tag="LR110_000042"
     CDS             complement(43781..45070)
                     /locus_tag="LR110_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4821049.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(45097..45399)
                     /locus_tag="LR110_000043"
     CDS             complement(45097..45399)
                     /locus_tag="LR110_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4821050.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(45424..47517)
                     /locus_tag="LR110_000044"
     CDS             complement(45424..47517)
                     /locus_tag="LR110_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL4821051.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(48006..48770)
                     /locus_tag="LR110_000045"
     CDS             complement(48006..48770)
                     /locus_tag="LR110_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBL4821052.1"
                     /translation="MKKINYLAIVLLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(48881..49750)
                     /gene="mdcB"
                     /locus_tag="LR110_000046"
     CDS             complement(48881..49750)
                     /gene="mdcB"
                     /locus_tag="LR110_000046"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBL4821053.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(49775..50851)
                     /locus_tag="LR110_000047"
     CDS             complement(49775..50851)
                     /locus_tag="LR110_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBL4821054.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(50875..51468)
                     /locus_tag="LR110_000048"
     CDS             complement(50875..51468)
                     /locus_tag="LR110_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBL4821055.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(51472..53148)
                     /gene="mdcD"
                     /locus_tag="LR110_000049"
     CDS             complement(51472..53148)
                     /gene="mdcD"
                     /locus_tag="LR110_000049"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBL4821056.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(53138..53437)
                     /locus_tag="LR110_000050"
     CDS             complement(53138..53437)
                     /locus_tag="LR110_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBL4821057.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(53539..55185)
                     /gene="mdcA"
                     /locus_tag="LR110_000051"
     CDS             complement(53539..55185)
                     /gene="mdcA"
                     /locus_tag="LR110_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBL4821058.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(55192..56103)
                     /locus_tag="LR110_000052"
     CDS             complement(55192..56103)
                     /locus_tag="LR110_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL4821059.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            56223..57080
                     /locus_tag="LR110_000053"
     CDS             56223..57080
                     /locus_tag="LR110_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL4821060.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(57267..57629)
                     /locus_tag="LR110_000054"
     CDS             complement(57267..57629)
                     /locus_tag="LR110_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBL4821061.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            57975..59414
                     /locus_tag="LR110_000055"
     CDS             57975..59414
                     /locus_tag="LR110_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4821062.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(59421..61973)
                     /locus_tag="LR110_000056"
     CDS             complement(59421..61973)
                     /locus_tag="LR110_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBL4821063.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(62087..63418)
                     /locus_tag="LR110_000057"
     CDS             complement(62087..63418)
                     /locus_tag="LR110_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4821064.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIDFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(63384..64862)
                     /locus_tag="LR110_000058"
     CDS             complement(63384..64862)
                     /locus_tag="LR110_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4821065.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(65167..67002)
                     /gene="tdc"
                     /locus_tag="LR110_000059"
     CDS             complement(65167..67002)
                     /gene="tdc"
                     /locus_tag="LR110_000059"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL4821066.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19130 a        14349 c        11525 g        22285 t
ORIGIN      
        1 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
       61 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
      121 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
      181 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
      241 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
      301 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
      361 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
      421 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
      481 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
      541 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
      601 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
      661 agtcgtagaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
      721 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
      781 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
      841 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
      901 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
      961 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     1021 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     1081 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     1141 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     1201 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     1261 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     1321 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     1381 ctgtacgatg atttttttcc aagcagattc gatcactgca gaaggtgctt ggctttttgg
     1441 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     1501 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     1561 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     1621 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     1681 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     1741 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     1801 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     1861 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     1921 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     1981 gatcatggtc gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     2041 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
     2101 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
     2161 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
     2221 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
     2281 aattagtggc tttctattcg gatggcttcc tcgaatgggt aaacttgctt atatctactt
     2341 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
     2401 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
     2461 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
     2521 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
     2581 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
     2641 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
     2701 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
     2761 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
     2821 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
     2881 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
     2941 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
     3001 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
     3061 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
     3121 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
     3181 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
     3241 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
     3301 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
     3361 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
     3421 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
     3481 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
     3541 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
     3601 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
     3661 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
     3721 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
     3781 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
     3841 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
     3901 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
     3961 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
     4021 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
     4081 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
     4141 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
     4201 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
     4261 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
     4321 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag
     4381 gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt
     4441 cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga
     4501 ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg
     4561 cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc
     4621 ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa
     4681 tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac
     4741 cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga
     4801 ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt
     4861 tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct
     4921 gattgattgc ctctgcaaat tgcgcgagcc attcttggat ttcttgaacg gttagtttca
     4981 tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta
     5041 aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat
     5101 ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca
     5161 acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc
     5221 cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa
     5281 caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac
     5341 cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta
     5401 cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct
     5461 tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga
     5521 ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt
     5581 caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta
     5641 attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa
     5701 ctccttttcc actatccgct tccttaatag ccgtctgaat ctgatctgga gttggagaag
     5761 taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg
     5821 atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct
     5881 tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc
     5941 cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc
     6001 catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata
     6061 ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa
     6121 cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta
     6181 ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg
     6241 attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc
     6301 tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta
     6361 tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta
     6421 acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg
     6481 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
     6541 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
     6601 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
     6661 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
     6721 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
     6781 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
     6841 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
     6901 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
     6961 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
     7021 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
     7081 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
     7141 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
     7201 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
     7261 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
     7321 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
     7381 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
     7441 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
     7501 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
     7561 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
     7621 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
     7681 tccgaggcac cgttttcagc accagaagag cttatagttg ttgaaggttc tgtttgagca
     7741 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
     7801 ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa
     7861 ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt
     7921 ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc
     7981 cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca
     8041 ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc
     8101 gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga
     8161 tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat
     8221 aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa
     8281 tactcaatgt ttgatcaatg aagatgatcg ttcttccaat ttgtatagat ttcgattgtt
     8341 ccacctagtc aatacaaatg aggtaatcag gaaactgaaa aaaatcaata caaaaaagcc
     8401 gattaagaaa tacagcattt tcttttctga taaaaaagcg tacaaggaaa aatccttggc
     8461 tctttctggt ctcgttaatg ggaaaaagac ccatataaaa ggaagaacaa cgatcatcca
     8521 tcctaaaaga gccactaacg tttgccaaat ctttaaccag taattacctt ttgaaaaata
     8581 cttatctttc accaaaattg atgcaacttc ttctcttgtt acttttttca tatcctcacc
     8641 tcacgttttt ttgttacgac gtaggagttt accatacatt tcatttcttt tcacataaag
     8701 tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata
     8761 ttttgttctt tttatcagtt gttcttttta ttttttatct cttaattatg ttgctctttc
     8821 caatacgaca atgcttttca ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct
     8881 ttgctcatct ccatcgtttt caaatggata taagattaca tgatcaaatt ccagtccttt
     8941 tgctagactc aaaggaatga tttggcagtt ttctaatcca tatgaatttt ttttaatcga
     9001 ttcttgtagc gtttgggttt cctcttctgt ttgggtgata actgccgctt tttctccttt
     9061 tagagaaacc aaaatctcag ctagtatttt caacaattct tcagaggatc gaaaagagcg
     9121 gtattcgggt tcttttcctt gcggtcggat cgatacgatg tcgatcttct cttgatccac
     9181 agcataggtt ttgaaaagct ctgtgattgc tccgctagaa cggtaactat ttctaagatc
     9241 ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt
     9301 ggaagaatta aagattgcct gattctcgtc acctactaaa gtaaagtgag tttttggaaa
     9361 taattcgatc aatagaccta attgtgccag ggtataatct tgtacttcat caatcaatag
     9421 atagcgaaat gctggtacat ttaccttttc aacaagtaaa tgacgaatag ctaatataat
     9481 gactgcttca tcaacagtac gtgtgggttg ccaagcataa gctgagccat atcgtttttc
     9541 ataaattctt tcaaacaact gatgctcatc cacccatgcc atttcttcga tttgtctggt
     9601 tatttttcga tatttttttt gtaaaagttt ccttgcataa gctacaatac tttgttccga
     9661 atcatcagaa atcaatttac cgaataattc caactgccta tcttctgtca acgagagtac
     9721 ctgattctgc acagcaggac tttttgcttg tttcaaaaga tgattcttcc attcgctcat
     9781 cagtgccttt ttcgttgctt gaagcctatc gatcaattta ggatgagcag gaactttctc
     9841 atatattttt tcgatcgttt ttttagaaag gactgttttt ccatttttag taagtccacg
     9901 gataaaattc ggatgatcta aaaacaaagg atctgattgt tttagattat cgataaactt
     9961 tttgctacgc aaacgttctg tctgctccga tacattttct cccgtgatac gtttgaaata
    10021 agcctcttca ccttctattt cctctgctga aagctgtgaa accagctgta tgatggttag
    10081 attcagtgga tttctttcac ctaatgatgg taggacattc gaaatatagt cgataaaccg
    10141 atgattaggt gatagaatca gcaagtcatc ggatgtcatc gtatctcgat ataaatagag
    10201 cagatacgca atacgctgca tgatagcaga ggttttacca cttcctgcta cgccatttac
    10261 caagatattt tgacttttca catcccgaat gattgtgttt tgttccgatt ggatcgtagc
    10321 agtgatagcc ttcatttcat tggtctcatc ttggcctaag acttctaata atacgtcatc
    10381 ttgaatagct acagacgtat caaaaaactt gaccaaacga tctctttcca aaagaaattg
    10441 tcgtctttct ttaataaccg tatcgatttt attttgacga accgtataag aagatggacc
    10501 aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc caatcataaa caagattttc
    10561 atctgcatca tctgtgaaat ttgctgtacc gatataaaag gattcttttg gctcattatc
    10621 cagaaagtct acaacgactt ttccaaagta gggacttttc aataaacgtt cgatctttgc
    10681 tagctgattt tctgcgtttt tctgtttgat attcatctga tcgatttctc tatttttcat
    10741 ttcaatcatc gaaaaagtat ccaggttatc taagtaactg tcaaaattca atcgaagctc
    10801 gccagccatt tctttcaacg actggtttcc ttctattttt gccgtatcca tcgattcccg
    10861 cagttcgttt tttttatcaa ttagcttttg atacacgttc ttcagatatg cttcttctaa
    10921 ctgtttttgt gtgttgacca tacacattct cctctttttt taaagtggtg gaaccgatta
    10981 gtttacctct tttaagagtc gaatgcaagc tttttaacga gtatcatttg ttaggatata
    11041 tcagttcttc aggtcggaca gtttgatata aagaaaatgc accatcaagg ttttttacgt
    11101 ggtagccaga ctgcttcaag attctttccg ctagatagct acgtaatccg ctatgacagc
    11161 tgacgatata ttcttgttgg ctgtctaatt catttaatcg gtcccgaagt tcattcaaag
    11221 ggatatgttt ggcttccttg aatttgccct tttccaattc ttctgctgtt cgcacatcta
    11281 ataaaatttt tccttttgct agttctgtcg gcaattcatg ccattgtacg gtttcactaa
    11341 gcccttctgc aatgttcatt gctgcatatc ctagcatgtt tactggatct ttggctgagc
    11401 caaatggcgg tgcatacgtc aattctaatt ccggtaaatc gaagatcgtc aaatgccctt
    11461 tgattgctgt tgctaaaata tcgatccgtt tatccacacc ttttgcccca acaccttgtg
    11521 caccataaat ctcgccagtc tctggatgga agatcaactt cagtaaaata tctgttgctt
    11581 ctggataata gcttgcatga tcttttcctg atacatgaat gacttcaaaa gacagttcgt
    11641 tttctttggc tgttctttca cttaaaccgg tggatgcggc agtcattcca aacgttcgta
    11701 taattgctgt tccaataccg ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa
    11761 cttgtctacc ttgacgattg gctggtgaag caagagaaat aagtgcatct tttccagtga
    11821 tttgctgttt tacaacgata gcatccccca ccgcataaat atcaggaata ttcgtttgat
    11881 aacgttcgtc tactaggata cccccgcgta tgcctaattc gattccagcg gtcactgcta
    11941 gctggttatc aggacgaaca ccaatggata aaataactaa atcagcaggg atggtacgcc
    12001 catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa
    12061 tcacttctac tccttgatga gacaattctt tttctataaa agctgccatt tcttcatcca
    12121 gtgttggaag aatatgtggt gcttgttcga tcaccatgac ttctaaacct cttcttttta
    12181 ggttttctgc catttccaat ccgatgaatc ctgcaccaac gatcacggca cgctttggct
    12241 gtttttctaa tgcatgcatc actttatcga tatctggtac atttctaata gaaaaaacat
    12301 tgtcggcttc tgttatccct ggaatcgatg gaacaactgg ttttgcacca ggagataaaa
    12361 ccaatgcatc gtactgttca atgtgttttt gaccctcatg tacgacttct accgttttgt
    12421 tttcgggaaa gatggctgtc acttcatgat ttggacgcac atctaaacaa aaacgagcgg
    12481 ataatgattc tggcgtttgg acaagaagat tttcccgttc actgatttct cccgaaagat
    12541 aataaggcaa gccgcaattt gcaaaagaaa catacggtcc tttttcaaaa acaacgattt
    12601 ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga cattccacct gctacacctc
    12661 cgacaataac gattttcatc ttatttccct cctactgttc gatcaaacca ttgattcatg
    12721 ccaccacgta cattcactac atcataacca gatttcttta attctttagc tgcttgttta
    12781 ctcctcatcc ctgattgaca aatcacatag acggtctttt ctttgtctcc atcatatctg
    12841 tttatacttt gtaatggaac atttttagct ccttttatat gcccgccccg gtattctgca
    12901 ggtgttctaa catcaagtaa cgctggattt tccaaaagct tttcctttaa ttctgggact
    12961 gttatcgatt ttattgattg aaatagcaaa ttcattttct gcacctcatt tacattttta
    13021 taaatacctc atggggtata ttaaacagga gctttattaa tatgtcaacc agaatgactc
    13081 ttcatttttt tcactacttt ttatatttgc ataaaatacc gataaggggt attatctaat
    13141 tataaaaata gaggagaatg tgttatggag attgataata aaaatatcct gaaccgtttg
    13201 aaacgtacag agggacaaat tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt
    13261 atggatgtca tcactcaatt aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg
    13321 atcgttgcag ataatctgaa aaattgcttt gagaaacctg atagtaatcc agaagaacaa
    13381 gcggctaaac tagaacaagc gatcaagatg attattaaaa agtaaaggaa tgatttcaat
    13441 gaaagtcgtt ttccatattg atgaattaga aaaatggtca gaaacaggga aaaacgtaaa
    13501 aaacctgata aaagcttctc cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat
    13561 taccggttat ctagactctg ccaatgcaga attcttagat ttgcaaggag tcgtttttca
    13621 cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa agttctttac ctgaacaagt
    13681 aatcgttgta ccagcaggtg tccttgattt agttgaactg caatcccaag gttatgctta
    13741 tatcaagcct tagtcagtag aggttgcgac agaagtgatc agcttcaaga aatttcggct
    13801 tatttttcga agaagctact tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc
    13861 tgcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc gtttggttgg
    13921 gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaatcagc
    13981 ggtttgacct aagtattaga gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt
    14041 ttgagctaat accgaaggtc agctgattga acaccgttta aggaagagat tgtgaaaaag
    14101 tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gatttctaca tttttgcata
    14161 aatttggctt aataccgaga aactggcttt tgaataccgt ttaaggaaga ggttgtaaaa
    14221 tcagcggttt gacctgagta ttagagaaac aaacggaaaa atagctgctc atattttgcc
    14281 gttttttgag ctaataccga aggtcagctg attgaacacc gtttaccaag aggttgtgag
    14341 cctaccattt aactccgaat cacaagaaat aatttcaaaa acaacttctc ctgtttttgg
    14401 tttggttggc ttattatcga gaagtagcta ctgggagtct atttatcaaa aaaaggaatg
    14461 cgacataggt cacacccctt ttttattttc tttgaaacat ctttcggtac tcgacagcat
    14521 acaagacaaa agattgtaaa agaccgtaag tgatgacggc aaaaatgact ctcacccaca
    14581 gcgttggtaa ttctgggaaa agcagcaaaa ggatcatgcc aacatagatg acagcagtcg
    14641 ctactttgcc ataccacttc gctccactta atttgatatt tttagatagc ccgattaatc
    14701 cagctacaaa cataaagcct tcttttatca agaatagacc aaacaacagc caaagccacg
    14761 gtcgatacca tgccatagac aaaatcagaa aaagttgtgt caatttatct gcaaacggat
    14821 caagcacctt ccctaagtct gttacctgat tatatctccg agcaatgacc ccgtctagat
    14881 aatcagacaa acctgagaca acaatgaccg ctcctgctat ataaaatgac tgtctcagca
    14941 caaaataaag ataaatcggc agcaaaaaaa tacggaatat cgataaaaga ttcggaatcg
    15001 tttgtatttc tttacgccag tctgtgctta atttcatttt atcgccctac ctcctcagca
    15061 ctcttttctt tttattttag cttacacggg gaataaaacc aatgatttca ctgtttttat
    15121 ttttctttac agccactgat attatgagct aatttgagat aatcacttgc tgttttcatc
    15181 gtttactctt ctttcttcct gtcgtttgcg catttctttt actagtactt tatgagcttt
    15241 ttttgatcct cgctctaggt cacgctgccg tttacgtgtt ttataatcga taaacagact
    15301 gtctaaatcg taacctgtcg ggtacagatc ttctgctgaa cgcaccagac gaacacgctg
    15361 acgaggcacg tggattattt ttttgtctaa atatacatca atcgtatctt cacctatatc
    15421 ttcataaact aacgcttctt tttggtattc attgacaaat atccgatcac cttttgcaaa
    15481 gagctggcgt tcttttttat tctccttcct atttgtaact gctgtcttag gaagaaattc
    15541 ttttttctgt gtccgatagg ctttttccgc caagtaacgc tctgcttgat ggatcaattt
    15601 catcgacata tccattttat gagcaatcca caaagcttgg ctttctcctg tttctccaac
    15661 ccgcaactga tatttaggac gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa
    15721 atcttcatga tctccggcga atttcttgat ttctccataa tgggtcgtgg caatgatcaa
    15781 tgccccttgc tcatacatag attccatgat tgcgatcgct agtgcagccc cttcatttgg
    15841 ctctgttcca ctgccaatct catctaaaag taccaacgta ttccttttta tctttttcaa
    15901 aatagcagcg atgttttgca tatgaccaga aaatgtactc aacgcatttt ctaaattttg
    15961 gtggtcaccg acatcgacga aaatctcatc aaagacagca agctctgtcc cttcttttgc
    16021 cggaacttgc ataccgaaca tcgccatcaa agtcaataaa ccgactgtct tcaacacgac
    16081 tgttttgcct ccggcatttg ctccggtgat aatcaagcct cgatagtctt taccgatttc
    16141 aacatccaat ggtaccgcat gctcaggtaa aaatggatgt ctgccttgct tgataatgat
    16201 atgttccgat ttattgattc taggtgtgct tccattgatt tctcggctaa attttgcccg
    16261 agcaaagatg atatctaaca ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt
    16321 ttctgcgata gcaccagtca atgccgccag gacttgatat tcttcactga tttcttcagc
    16381 ttttaacaac tgatagtgtt cattcaattt agaaatcaca gttggttcaa taaaaaccgt
    16441 cgttctttta tttgattcat cgataatcgt tccatctatt ttatttttat agctggcttt
    16501 gataggaatc gtatagtatt cgcctttttg aacaatcatt gcttcttgga tcatttcttt
    16561 gtttttcgga tgacgcaaga atttcagtaa ttttgactgg atttcttttt cgattagttg
    16621 taattgtttg cgtactttac gcaaattacg tgacgcgtcg tcactgactt tattattttt
    16681 tattttctgg tcaatcaact cttctacatt tatcaaatca ggaagatgtt tgctataagc
    16741 aaacaacaag ggtgtctggt attgattctt atcaaaaaac tttgtgatca tccgactgct
    16801 tctaagaaaa tccgcatatt cgatcaaatc ggttggctgc agaatcaacc cttttttgac
    16861 ttgttctgtc agtgcatcga tcctcggcaa ccccataaat ggaacatgct gactgctttc
    16921 taagatcaag cgtgcttctc ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt
    16981 cgagaccgtc atatcgctga ttcgtttttt gctatagttt ccaattgctc ttgcttggat
    17041 ttcctttttg atccgatcaa attgtaattg actaatcatt tcgttgtcca tattctctcc
    17101 ttttcttgaa gagatacctg tcccaaaata aataagggcc tagagataaa taaaaggata
    17161 gaacacagct taaaaaagtc atgtcctatc cttattagaa tacaaagata ttgttttcct
    17221 gacaggtacc gtttcataag cacaccaaat aaactttgtt tattggctat tcgacttact
    17281 gattagatac cagctacact cacaaaacat accccattct tctaggtctt atttattttc
    17341 cacttaacta tagcgagaca cattttggtt gtcaaacttt agtatgtttt atagatttac
    17401 accattaatg aatagacacc taccgcgata attgctccga tcattggacc tgcgattggt
    17461 acccatgaat atgcccaatc tgaagttcct ttattagcta ttggcaatac cgcatgtgct
    17521 aatcgaggac caaagtcccg agcagggttg attgcatatc ctgttggccc tcctaatgaa
    17581 agaccgatgg ctaagatcaa tccacctaca gcgaatacgt tcgttccagg tgcaaattcg
    17641 ttttgtccaa aagctagcaa cccaagaact aatacaaatg taccaattaa ttctgtgatc
    17701 acatttgctg gataattccg tatagcagga cctgttgcaa acgttcctaa aatcgcgctt
    17761 tcatctttcg ttgcattcca atgtggcaaa taagaaagcc aaacaagaat ggcccctgca
    17821 aaagctccta aaacttgcgc aatgatgaaa ggaacgacaa gattccaact aattgcccct
    17881 gtcatcgcca tagctagaga gactgcagga ttcaaatgag ctgggcccat gaatccagaa
    17941 atatagacag ccattgttac agcgagtccc caaccaaatg cgatgacaat ccagccagaa
    18001 gcctgtgctt tacttttgtt taaattcaca gccgcacaca caccatctcc aagcaaaact
    18061 aggatcagtg tcccaataaa ctcccccatg atttgagtca tatcactgtt catttatttc
    18121 tcccccttta gttcccgcag atctgattct gcaatcaatg cttctaaacg tttagtctgt
    18181 tgcgccttct cctcttctgt ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc
    18241 ttgatctcat caagctgatc tcgttcaaac aacagatgat ttgtacgacg aatgagataa
    18301 tcgcctgggg ccaaaaccat ttcttcttct aaaccataac gtaaccgagc tgactcagcg
    18361 agactcaaac caggataagg tgccatttct tttgctaatt caaagatacg tcgggcatta
    18421 gtcccgtaaa aatcagcgat ataagtagca tcttcttcct ctaaaccggc tgctactcct
    18481 tcttttgcta attctgtcac tgtttcttct aatttagttg gatcgaagtt tccacctgaa
    18541 atctgatatt ttttagagtc gatttctttc gtctctattc cgtattcttc ttttaataat
    18601 tggcgtatca atcgcaaagc tccttctgcc atcttacggt aatcggtgat tttcccacca
    18661 gataaagtca gcaaaccatc tggttctctt tctaaagaac tacctctgga aatcgtagaa
    18721 ggtgcttttt catcacggct gttttccaaa tggttcaaca cgtcttctac ttcagcacga
    18781 gaaactttgt tttccttata ttcacttact gtatcgacaa ctttattgaa gctcttgtct
    18841 gaaatcgatc cattatctcc accattgtaa tcagaaccag aattaccaat caacagtgga
    18901 cgaagtcctg cccagcttgc ttcgatatca ttcaatgtga tatttgcttc tggatagcga
    18961 tgattgatca catccaatag ataatctaca tcttcttgtg tgactttcgg atccgtaaaa
    19021 tctccttggt aatccgtatc tgtcgtacca aagtaagtct tgttttctct tggaatagca
    19081 aaaaccatcc gcccatcttg ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt
    19141 tttttcgcat ctacaactaa atggatccct ttagttggac gcattttagg agagactgga
    19201 cgcgtaaaat tcaagtttcg tactttatct acccaaggac cactcgtatt gatcactaat
    19261 tttgctttga tttcgatcac ttcatctgtc agcaggtcac gagctttgac gccaacgatt
    19321 tgatcgccat catataaaaa gccaaccgct ttcattttac ttactagata agccccatct
    19381 tcggcagcct ttttgatatt atcaatcact aaacgggcat cattgttgcg gaaatctaga
    19441 tagacacccg cgccttttag cccttctttt ttcaaaaacg gttctctttc caatacttct
    19501 tctggtgtca gcgtataatt ctcatatttg gttcctgtca catttgccag tttgtcataa
    19561 aggtccattg ctactttgac cgagaacata ttgaaggttg ttgctccttc gccctcatag
    19621 attggcaaaa gcattggatc tggttttggg atgtgtgggg caatactttg tacgactgca
    19681 cgttcaccaa ctgtgtcagc tactacttcc acatcaaatg tcttcagata acgaataccg
    19741 ccatgaacaa gtttggtcga gcgagaggaa gtcccttctg caaaatcctg catttcgatc
    19801 aatcctgttt tgattcctga tgctgctgac tggactgcca caccggcacc agtgattccg
    19861 ccaccgatga tcaacagatc caattcttct tgctgcattt tttgaatgct atcttgtctt
    19921 gtcttgtttg aaaacatatg gatttcctcc ttattcaaat gcttgggtag cttttactgc
    19981 ttttttccaa ccgttgtaaa gtttttctcg gcgctcttcc tccatttgaa cttcaaatag
    20041 cttgccagct tcataaaatt cgcggatctc gtctgtatct ttccaataac cgacagctaa
    20101 tcctgctaaa aatgctgccc ctagcgccgt tgtttccaag ttttctgctc gttggatcgg
    20161 tacattcaag atatctgttt ggaattgcat cagatattca ttgtttgctg caccgccatc
    20221 tactttcaat acaggaatct tgattccagt gtcatcttgc atcgtatcga tgatatctct
    20281 cacttgataa gcaatcgact gtaaagtagc tttaatgata tcttcttttg tcgtaccacg
    20341 tgtcaagcca aacatagccc ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac
    20401 gaatgcagga acaacataaa cctcatcttc gcttgtagag cgtttcgctg cgtcttctga
    20461 atcgctagct ttttgcaaca tttgaagtcc atctctcagc cattgaatcg aagaaccagc
    20521 cacaaaaata cttccttcta aagcatagta aacctttcca tttatgccgt aaccaattgt
    20581 cgtcaataaa ttatttttgg ataattgcgg tttttcaccg gtattcatga caataaatga
    20641 acctgtacca taagtattct tgaccatgcc tggttcgaat gccatttgtc cgaataatgc
    20701 ggcttgctgg tctcctgcca tccctgaaat tggtgcttcg cttccataaa aatgataacc
    20761 ttgcgtataa ccataaactt ctgagttaga agtgactttt ggtaacatca ctcgaggaat
    20821 atttagtaaa tctaaaatct cctgatccca atctagctga tggatattga acaacatcgt
    20881 ccgactggca ttcgagtaat cagtaacatg tgcttgtcca tcggtcagtt tccatactaa
    20941 ccaagaatcg atcgttccaa acatcaattc gcctttttca gctcgttctt gcgcgccttc
    21001 tacatgatca agaatccagc gtatcttcgt tgctgagaaa taggcatcga tcactaaccc
    21061 tgttttttcg tggaaaaatt cttgatagcc ttcatttttt aagttatctg caattccagc
    21121 tgactgtctg gattgccaaa caactgcatg atagattgga cgtcctgtat ctttttccca
    21181 tattactgtt gtttcacgct ggttcgttat gccaatccca gcaatctgat ttggtttgat
    21241 ccctgattcg atgaacgctc cggcaataac tgactgtaca gaattccaga tttcatttgc
    21301 atcatgttcc acccaacttt cttgcgggaa atattgcgtg aactcttttt gagaactgcc
    21361 gatgtgtctt cctttcttgt caaagataat tgctcgtgag ctagttgtac cttggtcaat
    21421 cgccatgata tacgttgatt ctgtcattgt gattccctcc attatatgca aacgctttct
    21481 ttaactactc ttatcatact actgttttaa aaaaagaaac tgtcaaaatt ttattctttt
    21541 tttctttttt tgacagtttt ttattatgaa gctatttttt aatcaattct tttctttttt
    21601 cagcgtctct attcttcttt tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag
    21661 ataatattca cactgttttt tgtttctctg gcaaaatgtg atcaccaaat catattcttt
    21721 accttcttca taacttccaa tctcaatacc gccgattccc ctcagttctc tgatcagata
    21781 ttgttgaaaa gcaatacgat aaatcggcag actttctaaa tcgattccaa tcactactgg
    21841 ttttgcaata taaaaatcga tcatcaaaag aacattggca tactggatca tcaggtaact
    21901 aagctcacta tcttcagctc ttttcctcgg caattgctgc accgctgttt cctgtaattt
    21961 cgccagtaac tgtgttaagg aatgcccgag cattttctgc tgctgttcca aaagccgatc
    22021 acgctcatag acttcgatct ttccaacaaa gaaaaaccat tcattgttga tctgggaaat
    22081 ttggtagcct actgcctttt cttctttgat tttcaaacga cgaggacgat aatgccataa
    22141 catcgtttcc cgaatgtaag tatctaaaac agcggttggt aactttttgc tccgtgtcaa
    22201 atcatactga taaaatattt cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa
    22261 catcattgtt tcgtatacat tcagttctgc tgctgttcga ctaagatgca tcacgatgat
    22321 tggatcgatt gcttgataaa gccggtccga ctgatagagt aatttctttt ctttcaaagt
    22381 tgcgtacgta cttttttcat tcaatagtct ttttttcgta atcccgagcc agcaagcaat
    22441 ctgtgcttct gctgatgccg taaacgttgt ttctaataca cggtccaatc ctagaatgaa
    22501 cggtcttttt tctggtgtat tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg
    22561 gaatagttca taataaaaat atcgtatctg tagctcttct ccgtataatt gaccgttttt
    22621 gatttgtaat ccaaattcct ctaagagctg gttcaattct ttgatctttc gaaaaacaga
    22681 agactcactg atattaaacg ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa
    22741 taaatagacg agcatcttaa acttcaagct ttcctgcaat aaaaacaata gaattttttc
    22801 caaacttaca tcttcgtcca acacaagcag cgcctgatac tcatttcgct gtacttctac
    22861 ttttttttcc atcattcggc caatcaattc aatatcttcc aaatattgat caaacgaggc
    22921 ttttgacagt tggacatgct ctctcaaacc attgatagag gctgttcccc cttgcagaac
    22981 aaggtttcgc aaaatcagga tctgtaactg ctcttttctt tctaataatg cttcaatctt
    23041 cataggcagt attcgccacc tttgaccaca ccatatctgg cacacggtat tctggctgga
    23101 aaaatagctc atgttcttct aactgttcaa acgtccatcc ttcttctact gcaattgtca
    23161 gttggtcaag aaggtggaac atctcttttt tgctttggag ctgtgctcca agaatctttt
    23221 tttcagaatc aaatagaaat ttgagtttaa ctttgctttg atttattgct gtaagcttgt
    23281 agtctacgta tcctgtttca attttttggg gataaaaaat gctctcttct tctgtcaaac
    23341 ctacgcttcc taagaaatag ccaaaccact gattgccgac tggacgatag gttccctgat
    23401 cctttgtctg tgagccacta attgtcttgc tgacatttcg tgctgtacgg acagcgttat
    23461 ttactagaga aacatacatt ttttcatttg ttggacgaaa agttacttgg atcgcatcgc
    23521 caatcgcata aacgtctttt tgagacgttt gcaagtagtc atctacccag atcgttccat
    23581 catcattcaa tgttaattgt tcttcccaca tctgattgtc ggggcgagaa tgattagcca
    23641 gcaaaacaag atcatagttc tcctttcttt tttccgtctc tacaatagcg gtttcatttt
    23701 cggtcaaaga cacaacttgt tcttctaaaa aaagtgaaag cccttgattt ttcatttcct
    23761 ttactaacgg ttctgtcatt tcaggatcta gataacgaaa aagaattgac tttcggcttt
    23821 cgtaaagatg gatttgtttt tttcttgatg ccaacccttc agctatttcg attcctgctt
    23881 gtcctgcacc aataattgca attttctttg cattcatgat cttttgctga agagccgtta
    23941 cgttttgtat ctttttaact gggtgaataa gcgttgattc tgcagaaata tttcgaaact
    24001 tttgatttga accagttgct agaatcaaac gatcaaaatc caattttttt cctcctgcta
    24061 aaacgacttt tttttctttc aatcctatta ctgtcttttc tgtgtaaaca tggattcgat
    24121 agcggtcagt cagttcttct ttcgtgatcc aagaaagttg tttttcactg gtaacctttc
    24181 cttttaatat caaattgaac ccgctgggaa tccatcctaa cttcgattgc ttttcaatca
    24241 aatatatttc acttgttggc atcaattggc gaagataaat agccgcatga atccctgcaa
    24301 acgacccgcc gacaattaca atgttcatct gcattcctct ttcttataag gtgttctact
    24361 gctaacttct caatcagaaa acacagtatt cataactcgc tttcaacctt atgatacata
    24421 gcgatgcagc agaatcaacc ctctccatca taccgtgttc taggtctgcc tctcaataat
    24481 gtactagtgg acctaattga agagtatcat ttctgaagag atgtttcaaa aaatgtactc
    24541 ttataaaaga aaaaaactcg ccttttacgt acctctgtcc aaataggaaa cagaaaagaa
    24601 cgaaaagacg agttttagcg tgtgtaatgt aatagttaga atagacacat catttatttt
    24661 taattcttta atgcttcaac aactgcttcg cctaattgat gagcagattg aggattttgc
    24721 cctgtaagaa gccgcccatc aatttgaacc acttctgaaa aagctgctcc agactgatag
    24781 tttgctcctt tttctttcaa agcatcttct gctaagaatg gtacttcatt cgttgtacca
    24841 tttgcttcct cttcttcatt actgaaccca gtcactgtac gtccagctaa aatagatttt
    24901 ccagactcat ctgtaactgg caacaatcct acaacaccgt gacaaacagc tgagaccagc
    24961 ccttcatttc gatagatttg tgaagcgact ttcccaagat ctattgcttc tggaaaatcc
    25021 cacattgttc cgtggccacc agcataataa accgctgcat aatcatttgc atccacatca
    25081 tcaggacgta aggtatttcc caaggcccga tttctgaaat cttcatcttc ataataggcc
    25141 caatcgattt catccatatt caaaacactt tgcggttcta aaggtacata tccgccttta
    25201 ggactgacaa aatctgcatc gatcccattt tttactaata catcataaaa atgagtcagt
    25261 tcacttaacc acaaacctgt aggacgctcg atcttttcgt acttttctac atttgttaat
    25321 acgatcaata ctttcatttt cccatcctct ttctataaaa attgcgttat tgataacgtg
    25381 ctttcaagaa attatataat gggcttggca aataaagctc tttatatgct tcttcaaaag
    25441 gtttcgttac aaattcatcc tcacggtttt ccaataattt ctctgcccag tcaggatcga
    25501 cgatcatttg ctgtccaatt gctgcaagtt ctgcatattt caatacctgc tctacatctt
    25561 cgcgattgcg gatcccaccg acaccaacta aagggatctt tccttgaatc gtgttatgga
    25621 ccacttcaag aatcgattta tcgttgtatt tatcagaacg agctattcga tcatacatat
    25681 ttaatgaaac atgcagataa tctagttttg actcacgtaa tcgttcaaga agccagatcg
    25741 tgtcttcaaa acggatacct ggtgtttcat attcttctgg tgagaaacga taccctacga
    25801 tgaatggttt tttagcatat tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa
    25861 taaaacgata acgattttcc aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg
    25921 gagagaaaaa ttgttggatc aaatacgtat ttgctccatg caactcgatt ccatcaaatc
    25981 ctgattcaat cgctctacgt gtggctttgc caaatgcttg gattgtttca acgatttctt
    26041 cttcactcat tgctcgtggt gtttccgcat caggacgttt tgctgcaact gcacttgcag
    26101 atacaggctg ttctccagat aaagttgctc gacttgtcat tcgtccaccg tggaaaatct
    26161 gaataatcgc ttttgcgcct tctccttgga ttgctttcgc tagctcactt aatcgaggaa
    26221 tcatttcatc atgagcgatg ctgagttcac ctggccagcc ttttccgata tcttggacat
    26281 ttgcagcacc agtaataaca gcaccaagac ctttagaacg ttgtttataa taagtgatct
    26341 catcttcggt gatcactcca ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca
    26401 atcggttctt caattgaact cctgaaggta aagtaattga ttgatcaaat tgtgacattg
    26461 tcattcctcc cataattcgc tctgattaac ctatcgagaa tcatctatca ttaaaccact
    26521 cgattagttg tacatgcaac taatttgctt aaaaaatttt attttttttg taacttatct
    26581 gttgcatcat tcattgtctg ggctaattct ttacttgctt catttcccaa aactgctttg
    26641 tatgactgat gaagttcttc ccagctacca tcgacttcct tagcaaattg gcgtccttgt
    26701 tctgtaagat aaactaatga caagcgacct tcgctaactg ttttgacgta tcctttatat
    26761 tctaattttt caacaaaacg agcaatcgtt gatggggcgg aatagagcat ttgtgcaata
    26821 tctttttgtg gtattccatc tttttctttt aatatcaata aaataaaacc ataagttggt
    26881 gccagacctg tccttctaaa cgcttcatca gctaatttat tcaacatgcg atctagcttt
    26941 ttcacgctaa agaaaagaca ttcatctaac gctttttcaa ttggcttttc catttcatcc
    27001 cctccattgg cgtacatatc atagtatgat ttttattttt tgtcaaaaaa cacggaataa
    27061 tgtccgcctc ctctgtctag atttgaaaaa aagaagtagc catcttcaaa gaatttcggc
    27121 ttgttttcga caaaactcct tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt
    27181 agttttgagt atgaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg
    27241 gcttaatacc gaaaaactgg cttttgaaca ccgtttatat cagaggttgt gaaaaagccg
    27301 tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt tgtcgttttt
    27361 tgagctaatg ccgatggtca gctgattgta caccgtttat tgagaggtcg tgagcctgcc
    27421 gttgaactcc gagtcacaag gagcaatttc aaaaaacagc ttctcatgtt ttatgactat
    27481 ggtgacttgt gatcgaggag ttggcaggag aatactgttt attcgtaaag aaggggctgt
    27541 gacaagactt ttgtcacagt cccttatttc cttttattat aggatttcgc catttgattg
    27601 gatgacttgt ctgtaccaat caaatgattt tttctttaac cgtttctttg tgccattccc
    27661 ttcgtcatct tgatcaacat agatatatcc ataacgtttt gacatttgtg aagtagaagc
    27721 gctgacgata tcaatgcagc cccacgacgt ataaccaaat aaatctactc catctttgat
    27781 agcttccttc atctgctcaa tatgctgttt caaatagtca atgcggtaat cgtcttgaac
    27841 ggttccatct tcttctactt tgtctatagc tccgagtccg ttttcagcta tgaacagtgg
    27901 tttttgatac ctatcatata attgattcaa agcaattctc aaacccttag gatcaatctg
    27961 ccagccccat tcacttgatg gaagatacgg atttttaatt cctccagtta ttaagtttcc
    28021 ttggacccct tcccaatttt ctggatgagc actgactgca ctagacatgt agtagctaaa
    28081 agaaacaaaa tcaacagggt aggctgctaa gatttcttca tcacctactt ctatttttaa
    28141 gttgacacct ttttcttcaa acattcgttg cgtatacgct ggataatatc ctcttgcttg
    28201 aacatcacta aagaacaaag aagttctttg ctgctcaatt gttttttgaa tatcatccgg
    28261 atcacaagaa tacggaacaa gaagctgata gctgatcata caaccgactt gtgaaccttt
    28321 gataatttca tgacaagctt ttgtagctaa agcacttgct ataaactgat ggtgtgcacc
    28381 ttggtatttc gcttgttcca aatggggatg gttttcttca attactccta cgctaacaaa
    28441 agggtgatgt tttacacagt tgatctcatt gaaagtcatc caatatttta ctttattttt
    28501 atagcgtttg aatactgttt cagcgaaacg aacgtaaaac tgaataagct ttcgattagg
    28561 ccatccacca tattctgtaa ccaaatataa cggcatttca taatgggaaa gagaaacgac
    28621 tggctcaatc ccatatttta gacattcgtc aaacacttga tcataaaaaa ataatcccgc
    28681 ttcattgggt gtttcttcat caccatgagg aaaaattctt gtccatgcga tagacatccg
    28741 atagcattta aaacccattt cagcaaataa acggatgtct tctttaaagt gatgatagaa
    28801 atcatttccg tgtcttttag gataataata cttgctgttg ggatcctgcg ccgcttttac
    28861 ttgctgatgc gtcatcatat gccattgatc tgtccatttt tctttaggta aatcgctgtc
    28921 gaatgtatac acatcagaca cagataatcc tttttcatct acatcccatg caccttcaca
    28981 ttgattggcc gctgtcgctc cgccccataa aaatccttct ggaaattttt tcatatcgat
    29041 ccccctaaac ttgctagttt tcttgtttga ttatttttct tatgactagt aatgatattt
    29101 ttgaatctac tactatttgt aacaataacc attgtggtcg gatcatatcc ctctttgatt
    29161 aattcttgca aatctacttc catcaataaa tcgcctttac gtactttttt tccatcagat
    29221 actgcacttt gaaagtattt gccatttaaa ttcactgtat caataccgat atggatcagt
    29281 atctctgctc cttcttcact gactacccca attgcatgtt tcgttggaaa aactgttgaa
    29341 acttccccgt ttacaggact gtagattttt ccgtccgttg gaatgatcgc aaacccttct
    29401 cctaaagatc cactagcaaa tgttggatcg tttacttcag ataaatcaat tatttcacct
    29461 gctgcaggag tacttaacac tatatccttt gaggattctt cttcgatttc atccttgtag
    29521 ccgaaaagaa aagttagcat acaggaactg atcatcgcta agaaataact agcaactagc
    29581 caaacaaatc cttcaccaat ataaatcggg aaagacaaaa tacttcttgt agcaactgcc
    29641 aaagcaccgg ctcctgcggc accagctact ccaccaaata tagctccaca tatacaagca
    29701 atgtaaaatg gtcgtttata tttaagagtc acaccataaa cagcaggttc agtgattcca
    29761 aataatgctg tgataaatgc agataaagat aaacttttta ctttcttatt tttcgttttc
    29821 aaaaataccc caagtactgc tcctgcttgc gcagctacgg cagtcataca tgctggacct
    29881 agcgtatcac gaccgtagaa agaaagattg ttggtcatta ctggtacaag tccccaatgt
    29941 aacccaaaaa tcactaacac ttgccaaaat ccgccgataa cagcaccaga taaaacaggg
    30001 ctaaagttat aaacagctaa atatccagct gcaatcagct gacttgcata atctgcaatt
    30061 ggacctacaa ctaataaagt aattgggacc atcacgatga tacaaatgaa tggtgtcaat
    30121 aaatttcgaa tactttcgtg caagcttttg cgaatcttag gctcaatata agaaagaacc
    30181 catacagcta aaattgctgg aataaccgta cttgtatacc ttgctagaat cacgggcatc
    30241 cctagaaatc gcaaggttat gctatcacta tatgcactga caatcgtagg ataaacgaga
    30301 gctgctgcaa taaccatagc gatgaaacga tctgtattga acttttttgc agcagtatat
    30361 gccaaaaata taggcaagaa atagaaaaat gcatcagctg ctgcattcaa aatgatataa
    30421 gttcctgatt cagtagttaa taaattcaaa ctcgtacata aggcgagtac tcctttgatc
    30481 aaaccacttc ctgccaagac gcctaaagtc ggggcaaaaa cagcggcgat catgtctata
    30541 aaagagttta aaatgccttt tttctcagtt cctctctttc cactagatgc atcatcttct
    30601 agtttcattt gtttggataa tacgtcatac acttcattta ctgcatttcc tactacgact
    30661 tgaaacattc ctccagcttc gactacagtg atcacacctt ctagttgctc taccttctcc
    30721 ttatctgcct tcttattatc ttttagggta aagcgtaacc ttgttgcaca atggtaaaca
    30781 ttgctgacat tttcttcgcc tccgacaaat cggacaatat ctttcgctaa ttgtgaatag
    30841 tccattttta cttccccctt gttaattaat gccgtagcaa tccttcttac aagataagta
    30901 tagttcttag aaaacggttt ctccattcaa ttagtacccc ttctaatagc gaacgaaact
    30961 gtacaatagt gtgtatggtt tacttagccg agcaaatcac tcactgcttg aaagaaaaca
    31021 agtatatcgt ttctcttata gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc
    31081 tgatgtataa gtggtatctc aagctgatac tctgtgattt ttgtttctac ttggcttttg
    31141 ataagacagc acaacaaagt ctcggcattt aacggaagca taactaggtc agaactagga
    31201 gagattagtt gctgaatgga atctatcttt ttatggattt ttatttgatt ttcttctttg
    31261 tagtaatgat caagcagttt tgttttcttt ttttcttcaa ctatggtcag cttttcacgc
    31321 aaaatctgat aaatctccca agattcaaac tcatctagaa cactaggtag ataaatcaaa
    31381 tcatcccact caaaaataag ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt
    31441 ttgtttgatt gatttttgaa aagatacact ggttctatcc ggcattcttt gatccacatg
    31501 ttcatacttt gttgaatctg tttttttagt gtattcaaaa tactaattgg ctggttacta
    31561 acaatcagta tctccccttg ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca
    31621 aaataagcgc tttcagctaa attgggacgt aattgaaaat gtattgggaa gaaacgccga
    31681 attaaataaa tttctaaagg ataacgggag atgatctttt gtacaaaatg attcatggta
    31741 ttatgtccgg catccatcct cgttttcatt tgtttgatat gggttagcaa aaattctgtt
    31801 tcatggaaca ataacgaaac aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct
    31861 cttagattct tctctatttc tttgtttttt tcatcattaa tcatcaaata atttaactct
    31921 aatagtcgat tttcaattgc ttgtttttct tgattggaaa aagaataggg tagttctgat
    31981 gataactttt ttgtgagggt ctctaccatc ggaataaata atgtatacct gtttgttttt
    32041 tctaaaagtt cttctcttcg aagaatggtc aaaacgagat accgagaaaa tcgtatgaag
    32101 tcctctcctg aaacaatata attatatgat ataagaacct cttttaatgt cttttggaca
    32161 gtcggcatga tattggaaaa aattgtactg atttgttgag gcaaccttgc gtgttggatc
    32221 accgtttctg ttccaacaag agataagaaa attctttttg aattttcttc agcaatgatt
    32281 tttagtcctt ttgtcgacga aacttccagt tctaatttag gattgcgctc gatccaacgc
    32341 aaaatcgttt ttatttgtaa tgatacggct gtttttgaaa gaaagaaaat gtctgctatc
    32401 ttttgcatac tgacataccc tttttgaaac aataagtacg tcacaatagt tgaagctcgg
    32461 aaatccatgc tggaatagat tttatgtttc gtcacctctt gtatgatttg ctgtttagtt
    32521 tcagattcaa ttgattctaa aaaataacct gaacgtttat ttgactggat aatgattttc
    32581 ttctcgtaga tagcaagcat atttttgatt tcagtttgga tcgttttctt gttggtacct
    32641 aaagcttcag ctaattcttc gcttgttatc caatgattct tatttcttaa tactacaaga
    32701 atttggagtt gtcgtttatt taagtccatt tgcttacacc tcctctttgt tataaacata
    32761 caggaaaaac ctctcaaatg acaatagtca cttgcagagg tttaaaaatc atttgaagaa
    32821 agatctatat tcaaggtttt taccattaga ctggataatt tgtttataat attcaaaact
    32881 atcttttttc aatctcctaa agcttccgct cccatcattt tgtctatcca cataaataaa
    32941 gccatatcgc ttcctcattt cccctgttcc tgccgatacg atatcgattg gtccccacca
    33001 taagtaacca atgatttggc aaccatcttt tattgcctca gcaatttcct gaagatgtat
    33061 ttttagatac tcacaacgtt ttggatcgtg gattcgattc aattcatctg gtgcttcatc
    33121 tagtccaatg ccattttcaa ctatgaataa tggtaattca taacggtctg tcaattcatt
    33181 catgacataa cgaaacccta gaggatcaat tggccaactc caaggttcgg gagatttttc
    33241 cttcaaaaag ggattatcga aaccatttgc tccgccagta tctacgcgca tttctgcttc
    33301 ttttttatag acagcgctac gataataact aaatgcaata taatctgccg tgttagcttg
    33361 caagcactgt tcgtctccat ctaacattac tggtgcacag tcttcttcct tccatgtcct
    33421 gtaaatataa gctggataat gccctcggca catgacatcg gagaaaaacc atgaattgcg
    33481 tttaaattgc agtgaaccaa atacattctc tggatcacag tcatatgcat aagtcgctaa
    33541 tgcagaaaag ctacacataa tacccatctt agcatttgga tcgattttct ttaataattg
    33601 agtagcacgg gcgttcgcta gaaacatatg atgacaggct tgataaatca ctttttttgt
    33661 tagtgtagca atgggttcaa gtttatctct agcagggaat gtaaccaatc cagcagctgc
    33721 ataaggcata cgaaaagcat tattgatctc attaaaggtc atccaatatt tcactttccc
    33781 ttggtagcgt tcaaagacgg tctgcgcata aaattcaaaa aagtcgatta attttcgatt
    33841 ttgccacccc ccatactctc ctaccaatgc taagggggtt tcataatgac taagagtaat
    33901 tactggctcg atatcatatc gatttaatgt attgattagt ctgtcataaa atacgagacc
    33961 agcctcattt ggcttctgtt cgtctcctct tggaaatatt cgtgcccatg agatgcttgt
    34021 tcgaaaagct tttaaaccta actccttcag taaacgaata tcctcttcaa atcgatgata
    34081 aaaatcaatc gcttcatgag aaagataatg gagtgattcg tctaaagcaa tctcccaaat
    34141 ctttttttct tcattccact gaatactagg atgctggttc atgatcccga taagcgtatc
    34201 tgtcacatta ggtaatttgc cgtcttccaa ccatgcacct tcacattgat tagcagctac
    34261 tgctccgccc cataaaaaat tttctggaaa tatttcctga tccataacta tctctcctcg
    34321 ctatcaacaa tttcttactt gaagtatagc tcgattcatc aaaacaagat attctaacta
    34381 tttcggcaga agagaagaaa aaaattgaat gctactcttc atttttaata tttatatata
    34441 acaaatacat aagacgatcc ccgaaagaaa acactcgtta accatctaag caaacgattt
    34501 attttacttc caacacaaat gcttcataag gtcgtaaatc aactttgccg tctggtaact
    34561 gtttcagagg ataatttgac acaataaccc ttaaagcttc acgtggaaca gtgaacgtct
    34621 gcttttcact ggacatatta caaataacga gccattgctg gtcttcatat atgcgagaat
    34681 aggcaaagac atgctcaggc gtcttttcca ctaattcata gtctccccag acgatgatgt
    34741 catgttttct acgcaaatcg atcaatttct tgtacatata aaaaacagaa tcttgatctg
    34801 ctaacgcttc ttctgcattg atttctttat aatttggatt gactcgaagc catggaattc
    34861 cagtagtaaa cccagcatgt tcacgggcgt tccattgcat aggcgtccta gcattgtctc
    34921 ttcctttcgc attgattgat tccaagattt cctcttttgt aaatccagaa gaaatccttt
    34981 cgttatacat attgattgtc tcaatatctt ttgcttccgc gatatcatcg attgggcaat
    35041 tcgtcatgcc taactcttct ccttgataga tataaggggt gcctttcatc atatggagca
    35101 agatagcaaa catcttggca ctttcttttc gatacacatc gtcatttccc caacgagaaa
    35161 caattcgcgg aagatcgtga ttattccaaa ataaactatt ccatccttca tcacctaaag
    35221 atgtttgcca tttggataat acttttttca gttcagagat actcaagggt ttcaaatccc
    35281 atttgtcttt gccttcttgc tgatctaatc ctatgtgttc gaattggaag accatggata
    35341 attcatttct tttaggattg gaataaagtt tagcgatttc ggtagttgct ccccatgttt
    35401 cgccaacagt catcagattt ttgtcaccaa aagttgcttg attcatttcc tgaagatact
    35461 catgcagttt aggtccatta cttgtgattt ctttatccgg ttctttgcca atcatatcga
    35521 tcacgtccat acgaaagccc ccaatacctt tatcgatcca gaaattcatc atttgataaa
    35581 tctcttggcg aacctgttta tttttccaat tcaaatcagg ttgacgcttg ctatacaagt
    35641 gcagaaaata ttgccccgtt ttttcatcca actgccaagc agaaccactg aatattgagc
    35701 gcaatctatt aggctctttt ccattgaccg gatcacgcca aatataatag tcccgataag
    35761 gattttcttt atttttacgg gcctcaatga accacggatg ttcatcagaa gtatgattca
    35821 ccaccagatc catgatgata cggatatttc ttttctttgc ttcttcaatc agttcttcca
    35881 tttcttccat cgtaccgaat tctgccatga ttgcttgata attactgata tcatacccat
    35941 tgtcatcatt cggagactcg tatactggag aaagccaaat tgcatcaata cctaaatacg
    36001 ccagataatc caacctggaa ataatccccc gcaaatctcc aataccatct tgatttgaat
    36061 cttgaaaact tcttggatag atttgataaa caactgaatg gtgccaccat ttttgttcca
    36121 tgattttcct cctataaatg gatgtttcta ttttttgctt aatcatttat tttagtctct
    36181 ctcactaaaa attgtaacgc tttcaaatag ctattgcacc ttgttatcgg taacagaata
    36241 aaacaaaaaa acagctgagg aaattccctc aactgttttt tgaaaggatt gatttatgca
    36301 gggaagaatg ctgatttcac aacttcttta taaacggtgt tcactcagct gtcccttggt
    36361 attagctcaa aaaacggcaa aatatgagga actatttttt cgtttttttc tctaatactc
    36421 aggccagaac actgatttca caacctcttt ataaacggtg ttcactcagc tgtccctcgg
    36481 tattagctca aaaaacggca aaatataaga aactattttt tcgttttttt ctctaatact
    36541 caggccagaa cgctgatttc acaacctctt tatttcacat tcaatatcca catgaatccc
    36601 acaaatacaa agaacagacc atacatgatt ggagatactt cttttcctct tttagctgct
    36661 aacatggtaa ttggatagaa gataaaacct aatgcaatcc catctgaaat actataagtc
    36721 aaaggcatgc cgatcaagat caagaatgaa gggatcgcaa tctctaagtt tccccattcg
    36781 atttgtcgca aagattgtgc catcaatact cctaccataa tcaatgctgg tgcagttact
    36841 tgtgaagtaa caactgatag taatggagaa aagaacaagc ctaacaagaa gaaaatccct
    36901 gtagtaatag ctgtgatacc tgaacgtcct ccaacggcga taccagctga tgattctaca
    36961 aatgcaccaa caggagaagt accaaataag gaacccgcaa gcatagctgt tgaatcagaa
    37021 gctaaagctt tccctacacg aggcatcttg ttgtctttca tgaaacctgc ttggtttgct
    37081 aatccaacta aagttcctgc tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg
    37141 acccacattt gaagagaatt gatgtcccct acatgtttta atgcaactaa aaatgttggt
    37201 ttcaaacttg gtgctgcggc aacaatttca gaaggcattg gaatcaaacc tgtgaccaga
    37261 cctaaaatcg ttgtagctgc catcccgatg aagatgccac ctggtacacg ccgaacaagc
    37321 aagatagcag ttacgactaa tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat
    37381 cctaatccta ctaatgtaga ttcattcgaa acgatgatcc caccttcgct cagaccaagg
    37441 aaagcgataa atagaccgat accgccagaa atcgcatatt tcaaatctgc tgggatcgca
    37501 tcaatgatca tttcccgcag cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg
    37561 aatacgccgg ctaatgctgt ttcccatgga atccccattc ctaaacaaac agaataagca
    37621 aaaaaagcat tgatcccaag agcaggtgcc gttccaattg gataacgggc taataccccc
    37681 attaaaacac agccgatcgc actggctaat gcagtcgcag taaacactgc tccttcatcc
    37741 attccagaag cacctaatac agttggattt acaaataaga tataagccat tgagataaat
    37801 gttgtaaatc ctgccagtac ctcacgtttt accgtggtgt tcaatttttc tatttcgaag
    37861 taggaaagta ttttttcctt catcttccat ccccctaaat ccgaacatta taaataaagt
    37921 ccgttaaaat ttcgatttat taataaaaca acgtgtttta tcatatacta aaacaaaaca
    37981 taagtaaaca aaaaattcgg attttaagca aaatccgaac gttccatctg attaaatccc
    38041 tttatctcaa agttcatgaa cattcacttt ggattacaag aatttcttta ttgtgttaat
    38101 aaagtttctt cattctagtc tcaaaaatca atagtgtgat ttgattttac catttcgttt
    38161 ctgttcttct ttgatgaatt cttttgtatc gacaggattc agattattta gtccgccaaa
    38221 acctgtatcg accatttttc cccagccttt tgtcttcttt tttcctgatt tgagattctt
    38281 tgctcgaagc ttctcactat gttttgctcg ccgatacttc aaataacctt ctaatcttcc
    38341 cattagatcc actgtttttc ctccttttta ttcaatcaga cgactcagct tttcctataa
    38401 caaaataatt gccttcaggg tcagaaaaat tgaaagtcaa gatgccattg ttatctgtga
    38461 tttctcctgg ttcttccatg ttctcgtaga tagtttcaaa ttcatccgta aaaaatacta
    38521 aagaaggcgg tggtcctaat acctctggtg aaaacttctg cacaaatact tttgggaata
    38581 cgcctaattc taattctttt gagatgctga gtacaatact cttaaattct tctggcaatt
    38641 cgattgtttc agaaatttct gcaccaattt tttctacaaa gaatcggcaa atcatctcta
    38701 cgtcattgac ataaagcatc acacgtgttt tttgtatcat catttgaccc ttctttctat
    38761 ttgattttct ttcctgtcta taaactgatt ccattatatc acgaaagcgc aaacaccttg
    38821 tgtttgatac tattcaagaa aaataaagca atagtttcta ggattgcttt cctccacaaa
    38881 ctttttttac aaaaaaaaaa acggcaatga atgccgctct tcttatttcg attcttttat
    38941 aattctgctt ctgattcatc tttggttagt gtgtgtctaa cactagttac gccaaatgtt
    39001 tgatcgattt tttccttcaa cgcttcgatt gcctgtgcgc cacgtgccgc ttgttcagca
    39061 ttgatggatt caatgaccag caccgctgct tgtgtttctt ccgtcagcat tgttctttgg
    39121 gcttcacgcc agttgagaca gcgacagatc gctccttgtt catcatagta gatgatttct
    39181 tccggaagtg ccggagcatc ggaatctgca cctaaaggca ggaatgattc gccaccttgg
    39241 gcttttccta aatgcagatt tcctgcgatc ttttcgatat cttctccgcc gcaaggtacg
    39301 ccaaaagaca aggaaatact attatagata tctacaagcg gattgattgg gaaaaattcc
    39361 cgttcttgat ttgcacgttt cagtaaagct tcaatagaag aacgtgcacc ttttttagtc
    39421 ttgaatgtgg taaatgcctg acgccactga gcaatgactt cgttttggct gaatgtttct
    39481 tctgtaaggt aatttcttgc ttcttttgct gcctcattca ataattgatg atacgtttct
    39541 tcactttctt ttgttacgtg gttatcgata ccatttacgg ataagatttc gatggttgct
    39601 tcaggaaata cttcccagaa attggtatcc actattactt ttttcatctg ttcacacctc
    39661 ttgattagaa taattgcttg atgaatagat agtctgtttg gggatcatct cccatataaa
    39721 aaacatgttc gctgtatttg ataaatcctt ttttacgata aaaagcgagc gcaacatcgt
    39781 tgtgttccca aacaccgagc caaatggctg ttttttgctt ttcttttgct ttttcaatag
    39841 ctgtatctag taaaaatgta ccgatacctt gatgtttaaa tttttttcgt acatagattc
    39901 gctcgatttc taaaaaatga tcagccattt cctcagattg actcgttcct gtatttagtt
    39961 tcaggtaacc cgcaggctct tgatcgatac tcactaggaa aaaatcggtt tctttatctt
    40021 ttagctcttc tgtaagtttt tctagtgcat aagcttgtgt caagtaatca gctaaatgct
    40081 ccggtgaatt atcttttcca aatgtatctg taaatgtttc gatactaatt tgttgtagta
    40141 agtctacatc tgaaggcgtc atttttcgaa tttcgtattt attcacggct tcttcctttc
    40201 aatagatcct tttattgcct tttttgacaa aattccaatc ttcgctaaca ttcttgcata
    40261 ctttatttaa aagatattcc aattgttttg cttcttcgtc agaaagacca tttaatgcga
    40321 ctgcattgga gtagttgttt tctcgaatga tgaaaggata gatttctgct ccttttttag
    40381 tcggatagag tttcttgatt tttttatttt ctttatcttc caaacgttcg atcatcccat
    40441 tggattctaa tttcttgatt gctcgggcag tcgttgtacg atctactttg atcatttctg
    40501 ctagtttttc ttggatgatt ccaggatttt cgcaaatacg tactaaataa agatactgtc
    40561 ctcttgttaa atcgacttct ttgaattcga tattgctaat agaatctagc gctcgggcaa
    40621 tcattccgat gtctcgaagg atttcagcca tttcatccca cctttatttt gtttgtaaga
    40681 ctagtttacc tctttttgtt gcaaatgcaa ctattttcaa taaaaaaatc aagttctttt
    40741 ttaataacta gtaaagtata ttcttttctt cttttacgaa aaagctcaat cacaatagat
    40801 ctggtttttg ttttctaaaa taagtaactg tttcttcgtt gttcgctttt ctgatagctt
    40861 tggcaactct tctatactaa aaaatcccat gttggaagtt tcattattct caataaactt
    40921 ggcctcttca ccgtggatcg cacaagcaaa aacaagttta taatactgaa aaagctgtgg
    40981 aatatctttt tgtttattcg tatcaaaaac tgctcgcaac tgagtagttt ctactgttaa
    41041 tcctgtttct tcatagactt ctttacgcac attttcttca ggagacaatc ctatttcagc
    41101 aaatccacca ggaagtgacc attctttcgt ccgtttatct tcgattagta ggaccttgcc
    41161 atcttttttg atataggcac gaacatctac ttttggtgtt ggatgtcctt ctccaatcgt
    41221 tgtgagcgct tttatttctt cgaaagtttc ttttcctatt tcactgatca gttccaatga
    41281 aatcgtccgg atctcctcaa aacgctcttt atcaaataca tcttttccat aatatagacc
    41341 agcttgagaa agtgcgagta gtcttttgta ttgagccaac ttcttgttca ttctcttccc
    41401 ttctttttta gcaaatgtat gactatgtaa tcaagtatag aagactggtt taattacggc
    41461 aaataaatat aatgaaatct gcaataaata aaagaatgtt aaaaaactcg agaacaaaag
    41521 acattcgact tctctggggc tgtgaataaa tggcgttcaa aagcttgcat ctgcggtaat
    41581 aagccccacc aaacgaaaaa tatgacgagc tattcttgtc tggctgttct tattaattga
    41641 ggcaggacag ctttttcaca acctccccac taaacggtgt tctcctacca actcctcggt
    41701 cacaagtcac aatattcatc aaacatgaga agctgttttt ttgaaattgc tccttgtgac
    41761 tcggagctga acgacaggct cacgacctct cggtaaacag cgttcaaaag ccaatttttc
    41821 gatattaagc catttctttg ttacttattg tcattgattt ctttttctaa tttatccaat
    41881 agtggagtgt aatccacttg agtatagtct gttacatcaa attcatatag tgttcctaac
    41941 tggaatttgt catattttcg gtcggcgatg atctttttga atgcttcttt cgtaatatga
    42001 ttggtccctt tcgttcgatc ttccaatacg tcaccatagt gataatgatc catgatatag
    42061 ctcaaatgac gatctgattc acgatcacgt ttgtaccgac gttgccataa cacttcaaag
    42121 tctgctgtta ggcgaatcgt gatcacctga tatccgtatc tatctgctaa ttcttggagt
    42181 cgtgcttttt gcttatcact aaaaggatac tcggacacaa tcactttctt tcctgcttcc
    42241 atatacaaat caagtgcttt gtaatagaaa ggataaactt tcgtcctttc ttgttccgcc
    42301 cgttcttcca aattgttaaa cccgatggat tctgcatagt attccttgat ttcatcgagc
    42361 gtgatggcat acatatctgg aaatcgttcc cgtagtttat tcataagata tgtttttcct
    42421 gtaccaggac taccagccag aagaatgaaa tactttttca tctcatttat ttcctaagtt
    42481 aattggactt tccaagttca taaacagtcc aatcttatgc ttgacaattt cttttactgc
    42541 ttcatttgct ggaatcacat tatggcgaag agacttgttg atttctgttt cactaaaggt
    42601 ttctttagct actttcgtcc agttgactaa aagctccgtt ccaacattga atttgcggat
    42661 cccgttattg atcaattcag gataatcttc ttctttcaca cctgttcctc catggataac
    42721 tagggggaca tcaactactg catcgatttc tttcagcaag ggaatgttta cttctgtttt
    42781 tgatttgaat tgtccatgat tcgtaccgat agcaatagct aatgcatcca cgcctgtagc
    42841 ttctacaaat ttcactgcgt cttccggtct tgtatatacc ttgtcatttt cagcgacatg
    42901 gattccttct tctgtaccgc cgattgttcc aagttctcct tctacggaaa caccgtgcgc
    42961 atgggcatat tctactactg cttttgtctt caggatattt tctttgaaag gcagatggga
    43021 cccatcatac atgacagaag tatatccgct gtcgattgct tctttgatat catcaaagtc
    43081 tcgggcgtgg tctaagtgca agactgcatc aaccatttct tcttctgcaa tagctgtcat
    43141 tacattgact aacacatgca tgccgatata tttagcggta tcgacacttg tttgaataat
    43201 gatcggcgaa cccatttctt ttgctgcgcc tagcatagca ggaagcattt ctaggttatg
    43261 cgtattaaaa gctccaactg taaaattcaa ctcttctgct gtttttgtta cttctttcaa
    43321 tgttgtatac atcttttatt gccaccttat ctttttattt gtttcctttg atcgttactt
    43381 gttttgcaat ttgtcgcatc tcatccattt tgcccatgta gtaatcgatc ccttgatcat
    43441 caccagcacg agcagcatct gcacgcttct ggttatagag gctcatcagt tttttatacc
    43501 catctccgat cgtctgttta ttttccaaac tcttttctaa atattggatg gcttgatcgt
    43561 tcacatacaa ttcagaaaat ttaagtccaa cttgttcata aagtttcgct aatttatgct
    43621 tatcttgggt agtggaaatc tcttgtttca attgtgtaat ctctgattga atcttttcgg
    43681 tcgcttctgg cgatagggct ttttcttctt ttgccagaac ttttttcggt tctttttctt
    43741 ttttcttaaa aaatccaaac atcattttcc ctactttcaa ttaaaatgct ttcaaaactg
    43801 ggcttaagat ccaggcatat aagagtgctg cggctactgt atctacatcg gttgctgtag
    43861 cattgatgaa tcccatcgaa ttaaagatcg ttactagcaa agcgggcaat aatgtaataa
    43921 agaaaccatg agcaataccg ccaatgatcg ctcctcggcg tccgccaact gcgttgccaa
    43981 atatccctgc tgtccctccg gcaaagaaat tggttagcat accaggtaaa atcatcgcta
    44041 agccgaacat gggcaaagta aacatggcaa taatcgtacc aatcgttgtc gtaatgaaac
    44101 ctaaaatgac agcatttggg ctataaggaa agaaaactgg acaatctaag gcagggatcg
    44161 catcaggaac taatttcata gctatccctc gaaatgccgg cacgatttca cctaacagta
    44221 gacgtacacc agctagcaat acatacacac ccactacgaa ttggatggct tgtaaaaagg
    44281 caaacatgat atagttttga tctcctgata attcagaagc gaatggttcg cctgcaaata
    44341 aaacagtgat gatatatagc gggaccatca cgaccatgac agacaaataa gtatcttgca
    44401 gaaattcgaa atgtgtcggc aacttgatat cttcgatcga ctttttattt tcgcctttct
    44461 caccaaaaag cttagcaaca ccggcttcaa ataagtaccc gatcgtacaa aaatgaccta
    44521 aagcaatatc atttgatcca gtgattttac gaatgatcgg ttgcgctact gccggcatag
    44581 caatggcgaa acaagctccg ataaaaccac cgaccaagat caatacgatc cctcgcagac
    44641 cggcaaaata gccaaagact gtcgtcatcg ttgccatcca taaaattgct tgaccagtta
    44701 aaaaaatgta cttccatttt gtgaaacgag ccaaaataat attgaataca aaaatggcta
    44761 agaacgtcag tgcaatatct ctacctaaac ccaaatcatt cattgcttga ccattgatag
    44821 attcaataga agggatgatc ccttgcatat gaaatccttc agtaaagatt ttcccgaaat
    44881 aagttaaact tccaacgatg atgctcgaac cggcactcaa cacttgaaat cctaggagtg
    44941 tctttaacgt gccagagatg acttgtcctg ctgatttctt ctgcagtagt agacccaaca
    45001 tagcaatcaa tgcaatggtg atcgatgcct gagtcaaaat attttcgatg ataaaattga
    45061 taacgcccat gtttttaccc tcttttcttt ttgtatttag ataactccct tatcttgcag
    45121 aactggggtg attttttctt caatttcatt ttttgaaaca atatttttca aatagatcat
    45181 cgccgtttta ggatcaactg cgaacttttc gaactgcgaa cgaaaatttt ctgctgtgat
    45241 gatcacatct ggtttcatgg aaactgcgga agaaatatca caatggtcca gtttggcttc
    45301 tactcctaat ttgttcaaaa catcctcagc agacatctgt gctgcaaaac tacttcctaa
    45361 acctgcaccg catacgaata aaatgttcaa tttgttcatc tttgttctcc cccgtatcat
    45421 ttattatttc tcaactactg aaaaaagcag ttgtttgaaa tcttcgatat ttgtgcattc
    45481 aatcaatcga tccaacttct cttcgttgtt gatcaattca attagttctt tcatgatgtt
    45541 cagatgcgag taggagtcta ctgctgccaa acaaaaaata atctttactg gatccatttc
    45601 aggattaccg aaatcgattg gttgctcaat cgtagctacg ctgactccta atttattgac
    45661 cccatcttct ggtcgggcat gggctagtgc catgtgtttg ccaatcacga tatacggtcc
    45721 gtaatcattc actgcacgaa tcatcgcttc gacatagcgc tcttcgacaa tgttttcttt
    45781 aaccaatggt ctagcagcta gttcaatcga ttcctcccaa gtagtggctt tttctttgat
    45841 catgatattg ctgtccgtca aaatatcttt taacataggc tggatctccc ttttgttgat
    45901 ttctaaattg tttgtttcga ataatttttc taacttgcca tagatgattc gatctactcg
    45961 gccaccactt tctttcatga catctattat ttgagtgaat aactccgtcg attcatgact
    46021 gttaggctct aaccgctgta gttcttcatt tcttttcaaa aagtcttcta taaccaagat
    46081 attcgtctca gttaaaatcg gatcgacaac taaaagcggc ttatttcgat aattcaattg
    46141 aaaggtgctg aaaaccagat tgacatccaa tttatctact aattcgattt cttttgaact
    46201 taaaactgca gtaatctcga tctgaggaaa cttttctttc agattctctg ctaacagatt
    46261 tccagtagcc atcccatggt tacagacaac gactgattta taaacataat tcaagtctcg
    46321 cttgattaca ctggcgatcg tcgaaaagtg gatgacaaga aaagctatct cgtcctccag
    46381 tatcttgcca ccaattactt cttcgatcgt aggaacaaaa tgagtgactg cgcggtagat
    46441 tgggaaataa tttgttcgga tgttttcttt caatggattc atgacttgga tatgattctt
    46501 gattcttaca atcagtgcag ccatgtgctt atagagattt tcgaataaag cttcttcctt
    46561 tagatggaaa ggtacgtgtg tctcgttctc tacaaattgg atcaattgga tcgttagtaa
    46621 ttgagcttgt acccactcga ttgaattgga gatatctcta gcattgaacg tctcaatagt
    46681 gaaacgaaga taatttcttt ctacttgcgg gacattgtcc aattgaaatt cctcgatcat
    46741 gtggtcaata aatgcagaaa agctactgtc ttttatttct gtatttttcc aattaatggc
    46801 agagataaat tcatgtttcc tgattcgctg gatccagatc aatgtaaaaa gcaggatttg
    46861 ttttttataa atatcatctt cttgtttcaa caacttattg gaatagatct cctgtatttt
    46921 tttgatttcc accaaaggaa aataatggag aaagattttt tgagccggtg ttaattgtgc
    46981 atcttgattc ataaagaagt cggtcattcc cagattctga ttgataaaat catagatcat
    47041 cgtacggata gatcgttctg aacctttata aaccagtcct tgctttccat aactgacgat
    47101 ctctatccca taactgacca gtgttgctcg taaccgtctc atatcttcat ctaatgtact
    47161 tttagagatt tgaaattctt cttctttttt gtttagatgg atcgggattc ggctgtaagc
    47221 aacggctaaa actagatcaa aaatacgttc gcgccgattc aaaaagccgt ctttcacatc
    47281 ttttatttca gtttctaatc gagcttgctt ttccttttcc agcttcaaca aaaaaccttt
    47341 gttgcgaatt gtacggatct ccggaagctt tttactgctc aaccagatat tcgcttcaag
    47401 aacttcctga cggatcgtcc gctgactgac attgaactca cttgctagat gttttgcttt
    47461 tactggatat tgggctgtca tgattctgtg aagtatttca attgttcttg ttttcataga
    47521 aatgatctcc ttctgatttc aagtatatgg ttccgcttac ataataaaaa gtagatattt
    47581 ctgccacttg aagtggaaag aaagtaagca aagcaaacaa aaaataagcc atatgctttt
    47641 tcatctcctc aacgaaaaag acatacgcct attattacaa aaattttgcc catctataca
    47701 aaaaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacaacta aactaaaata
    47761 gtgcttccta tttttcgttt ggttgttctt attatcgcag atgcaagctt ttaaacacca
    47821 tttagtggag aggttgtgac agagatgttc agccttgagc gataagaagc gattttgaaa
    47881 aatcgcttcc catattttac aaaatcgtgc cttattgccg ataggctatc tctggcacac
    47941 cgtttattca gaaaaaggag ctgcgacaag gcttttgtca caactccgtt cataaatatt
    48001 tcctgtcaat ccagcataat cggctgaata aaatcggctt gccatctttt ttctttctca
    48061 gaaaaatcaa aataccatat ctgagaaaaa tactgtcgtt gtgtcttact tcctgagatc
    48121 aatcgcccat cttttcgatc gactgaataa tcacagcatc tgaaatcaat tcggatggca
    48181 aaactatttt cgtggatctg tcgataattt cctacttcct gaatagttat ttttttagta
    48241 tgatttctta caccgtctgc tttgtttttt tgcagaactt tctgatgttc ggcaaacagc
    48301 ttgtctgtat aacagcttct ggctttttca ggagtttctt cttcccaagc ggattggatc
    48361 ttcatgaata tcttttggat ttccagaaac tgtgctttct tttctttagc cgttcctgac
    48421 atgcgggaca atatctccgc tttcagcctt ttcttttgtc ttttattctt gacgatagaa
    48481 aaagcagaaa cccctcctac tgacaaaaca gctaatgcaa gaaatcccgt ttgctcataa
    48541 ctagaaggac ctgtacctgg accataatac ccataagaat ctctgccgta agaacgactg
    48601 ccaaaataac tgcccccata tgaacgacca ccaaatgaac ctctgcccga acttcgaccg
    48661 ctagatgtac ctccaccatg accacccgcg acagcgaatc cttgggtcgg cagagcaaac
    48721 aaacataggc ccactaacaa aacgattgct aaataattga tttttttcat tgtcttcctc
    48781 gttatctttt attacaaaaa actattttta tgatagcctt gctaaaagga agagtcaata
    48841 accagatgca taaactgttt ttttagcaat tttgtcttat ttagtcattg agtaacatcg
    48901 gacaaatgta tgtgtcggca gtccataatt cctctatctt cagcatgaac aagcttgcag
    48961 acaagagatc tgcgcttcct ccaggagaga tttggttttc ctgacaaaag tgcaacagtg
    49021 ctgtaaatgg acgatttggt aaagcacttt gactagcagc atgagcaagt ttttgcattt
    49081 ccactaacgt tttctgatca ctgcgatgca atacgcatgt gtcatctagc gaactcatca
    49141 aaactaacaa catatgcatt tgtttctgta catatgaatc attatctagc tccttagctg
    49201 cttggattgc aagcctgata tgcggatacc ctaatatcgc ttcttcatat gcaccattta
    49261 cgccatattt ttgcttcacc ctatgaccat gagtggtttt tttatgacga tattttgtat
    49321 caggaaaagc agcaagcttg ctgacatcat caagtatttt cgataaagaa aaaggctgtt
    49381 ccatactgat tttttgtgca atgacactga tcaatagccc cattgcccaa atcgctcctt
    49441 tatgggtatt gatgccattt gtagcaatca gcattgcctt ttccgctttt cgcccaatct
    49501 cagcaattct ttctcttaac tcctgatcaa tagcatgatg ccatgctatt tgagccattt
    49561 cttcaaaaaa aggagttagc gtcttagcag acttaagaaa taaagcatag tccatatcgg
    49621 aatgtgcacc cgcatccagt gcatccacta atccaggttt tggacttaat gttacttctg
    49681 cgatcaatgc ttcctctacc aacgcagcga gttgctgagt cactgctttt tttcgttcgt
    49741 catttatcat gacactcact ccttcttcct actattactt gctgaaacct tttttgatag
    49801 ctacccaaat gatatgaagt aaccagctta aagcaaatgc acaacttgcc gtcattagat
    49861 aagctacaag aatcatgact cctgaaggta actgcagcca actatcttta aacaataata
    49921 acagtacttg gaaaacaaat acattggcta aatacgtacg ataagcataa acagacaacc
    49981 atttgaacaa tggcaatttt ggcgaatcgt ttacaatcat tttggaagcg cctatgaaga
    50041 cagcaaagat gatgactaaa ctgtataaac tttggatcgt attcaagtaa ggtgcattcg
    50101 caaagctcaa gtctcctgga tgtgcgagca atgaacggac agaaaagaac gcgataacta
    50161 aggcaacagg taagaaaaga taacgtactc tgtccaaata ttgatagact ttttcgtgat
    50221 aaacaaatgc ggctacccca taaatcatga aactagcaac aaaccgatct aataagtacc
    50281 agctgtcatg ataaggccca gtcaatactt gtgtttgata gaacagatac catgcgacat
    50341 ataaaatcgt tgaaccaatc aatactggcc atacatattt tttattagca aatactttgt
    50401 aaccgagcca aacaaaaaac ggcatgaaca attggatctg cagcatcata acggtatacc
    50461 ataaatgagg cgctccatca cctgtgacat atttcagtaa aaagcttgca gtatccgtat
    50521 acggagtttg ttgttggact gctggaaata gccaaagata agccgtcgtc catagaagat
    50581 acggaacgac tagttcaaaa aacttttctt tcaaaaattc ccaataagac aaatgctgac
    50641 ttgtttttac catattataa acaattgcaa aaataaacat tggtgctgtg tatttaacga
    50701 tgacgtaaaa ggtaccaaga aaagctgtat ttgttgcgct gtggctgttt gccaaagcaa
    50761 agctcaatat cgtttgcatc ataacagccg ttacagctga tactttctgg aagtcactta
    50821 cgttgatttt gatttttttg ttttcaaaca tctctttctc tctctttttc tttattacca
    50881 aagttctttg gttaatttag gtccttcgac tgttttcaat agaatttttt tctctggctc
    50941 tctcaaatac tcttttagtg aaaaaccgcc ttgccgagtc actacttggg tatcgaattg
    51001 ctcaaaaaaa gcaggatgcg attgaattac ctgaattggc aattcaatcg gtgaatttgc
    51061 gtacagcagc aaatcaaaat cactggtttc ttttactgcc ggaatcccag tcgataattc
    51121 aaatcctaag ctgccgccga ttccccattt acatcctgat aatatgtttc tagcagcatg
    51181 aaattgctga taaacaggat aatccatgat tttctcttgt ctatacgatt cttgttcgat
    51241 gatgtcccac ggatgaatga cttgtttgac ggcatgtttt gagatttctg ttgaccatct
    51301 ctgattgcgg gcaggtccac gtaaaccaat cggtagtatt tctgcgtgat ccgtcgacgc
    51361 acgacgaact gttccgaaca tcttttcagt tagaacgacc cattcaggca aggtgaaccc
    51421 ttctatttca tgtttatcaa aaaggacaat atcatgtgct tgaatcattt tttatgcatc
    51481 ccattcttcg tccattttgg cacggacttt atttgtttcg acacgtccga atgttttggc
    51541 tgtttcgttt gtgtatcgat ttgtcagcat cgtgtcattt gtattgcgga catcttgaat
    51601 ggcttcattg attgcttcga tgactttgct gacattttgt tctgaagcag cttggtcggt
    51661 gatatcttct aggaaatgat agatcgctcc tagttttgta taattattga tatcgtaagc
    51721 gatagctgga actttttcag cagctgcttc gatttcagca accgaacgtt ttgtcacacg
    51781 tgcagcactt tctttagaca tcgcttggac ggtaatttct ttattgttta aagcaatcaa
    51841 gcggcttgat tgtaatccat gtgctaaaaa tgcacctgaa atagcatttc ctgcaatcaa
    51901 tccaataact ggatgtccag cttgacggag ttttgcataa gcagcagcac tattagccaa
    51961 agccacatgg ataccgatca attcttcgtt atagccgtat gcttggcttg gtacatcgat
    52021 cacaacgatg attggccgtt tcactttttt gtctttatct tcttcgtaaa cgtgatccaa
    52081 aatttgtccc atgcggaaac cttcaactaa accaacttca ccgtttcgta cacggtacat
    52141 cgggttttta ggatcaggta cgattgatgt cacgatggct tcttctatga atttacttga
    52201 tttaccataa tacacagtac taatagaaga agttggattt ttcatgccag ttagtgccgc
    52261 aaaccaatcg taaccaacac ttgctgcagc tggttcattt ccttctgcta cttctggtaa
    52321 gtcaattgat tttgcttgat ggttagcaat tgcttcattg taatcgtcaa tggccattgg
    52381 ttttgaaaga tctaaagaat caagtaatga taaataaaat gctgagcttt cactgcgatg
    52441 cgtgtctttt cgttcgtcca ttgctgcgat gacagcagct ttgattgctt ctacactatc
    52501 agtcactaat tcatcaatga tttttgattc tacccgttga cgagatccta atgtattcca
    52561 aatcaagtct ttgtcactag aatcaaattc tcgaacgcct gcttcttgtt caataacttc
    52621 tggtccattc aaaccgactc tcgctttttt tgttgcgatt aaataactca ttaatgctga
    52681 agtaatcgac ataccgccaa atgatcctac acgtcctgga atcaaaccga tgactggtac
    52741 atattgtttt agagcaatga ttacatttcc gatttctgaa atagataaca gtccgtagtt
    52801 tgcttcttgc aaacgaacac cacctgtatc tagtacaatg actggaaaga tctcatttcc
    52861 ttttttattt tcttctaaca catgttcaag tgcagcaacg atttttgcac cggaaacttc
    52921 accgataccg ccaccttgga attttccttc catagcgatt actactgctt gtttgccatt
    52981 gatcgtgcca cggctgacaa cgatcccgtc atcactttca gggacgattc cttgtgctgg
    53041 taaacttggc gcgatgattt gatcgaatgg atcaatcaat tcacgtccat tgtcaagtag
    53101 agcgttcact cgttctctgg cgtttaattc tacaaaacta tttttcatct gaaagttcct
    53161 ccattgcttg cgtcaatcgt aggtacacaa ctcctggtgt agcaccgaag tcattgatca
    53221 cgatatctgc tgaaattgga taacgatcaa aaaatctcgt taatacattg ttccatactt
    53281 cgccgaagcc atcgcttcct gtacaaattt ttacagtaga ttctgttttt tctgttggaa
    53341 acatcaagat ttccaaatct cctgaaccga ccactcctac atggattttc ttattgatcg
    53401 gctgtttcgt tggatattta taagctaatt gttccatgga tctctcccct ttttgtaaaa
    53461 cagagcatac acgttctgct tattcgtctt tcttcttcca tctgggtaaa tggcgacaaa
    53521 aggtttttct cgccaatttt atgaccaagt tctgaatttc attggtggtt catacaggcc
    53581 atctgaccat tcgatcaaat catcgatggt ttgtgcagct aataatgaac gtttcgcttc
    53641 actacgtttg atgcctagat cttctggtaa tttcacgatg ccggctgcac gcaagctatc
    53701 tagtgattct ttcgtgctag tcattccaag aggtgtaaca ccgccaattg ctgcaattgc
    53761 tgcttgtcgt tcttcttttg tttttgcttt gtacaaataa gcaattcctt cttctgtcac
    53821 gatatgtgta gtatcttcgc tataaatcat cactggtgca ttggctaagc ctgcttgttt
    53881 ttgaacagct agtgcatcta attcttcgac aaagactggt tttttatttg aaccatatgt
    53941 ttcaaccatt tgaacaacta atttttggcc tttccctaat ggatcatcat tgtcgcgaag
    54001 tgattgccat gctggtgtag aatgacgacg accgccagga ttatgtccca tgttaggcgc
    54061 accgccaaaa ccagataaac gtccttttgt tacggtagaa gagtttcctt cgtaatctaa
    54121 ttgcaaggtt gaaccgataa acatatcgat ggcatattgt cctgcagctt gtgataatgt
    54181 tcggttactg cgcatcgtgc cgtctttacc gacaaaaaag acatctgggc gagcagcaat
    54241 atatttttcc ataccaactt cgccaccaaa actatggatg ctttcaaccc aacctgattc
    54301 gatggctgga atcatcgttg gatgtgggtt caatacccag ttcttcgcga ttttcccttt
    54361 taaccctagt gattcaccat aagttggcag taacaattcg atagctgctg tattgaatcc
    54421 aataccgtga ttcaaagatt gtacgccgtg ttctgcgtag attcctttga ttgccatcat
    54481 tgccattaga atttgcaatt ctgtgatgtt ttgaggatct cgtgtgaata aagcttctaa
    54541 ttgataaggt ttatctgctt tcacgacaac gtctacccag tcaccaggga tatctacacg
    54601 tggtaaagaa tcgacaactt cgttgacttg aacgatcacg ataccatctt tgaaagctgc
    54661 agcttcgatg attgttggtg tttcttccgt attgaaacct gtgtacaagt tgccgtcttt
    54721 atctgcttta tctgcagcta ctaaaacaac atttggaatc aaatcgatga ataagcgtcc
    54781 ataaagttcc aaataagtat ggatatcacc taatttcatt gtgccgtctt caatcatttg
    54841 ggcgacacgc aagctttgtg caccagcata agagaaatcg atcttgcttg cgattccttt
    54901 ttcaaaaata ttcaaatgtt ctggtcgtga gatactagac atgatcaaat gcagatcatt
    54961 cactttttct gcatctactt tttcaagact ttctgaaaga aaacttgctt gtttttgatt
    55021 gtcgccttct aatacaactt tgtcgccggg agcaatcagt ttttccatta cttctacgat
    55081 tttttcagtt tctacatact tgccattgag caagttgctg attttttcca atcgctgcgc
    55141 ttttttttcg cgtttagaag cccagttgcg gtttggtttt tccataatta gtcatctcca
    55201 tttttgatat agaaacgtgg cgtcatcaat accatgttgg tctagattga tcgtacggat
    55261 ccctttatct acaccaaatt ttgtatgaat cagctttgtc aaagtattgc cggggggaaa
    55321 ttcaatcgtc agatccattc ctaattcttt tgccaagtcc atcatttggt tccactgcac
    55381 agggaaagct aggttttcca acaaatcttt acgaatctct tctgcgtcct gagtggctct
    55441 tccatccgta ttttttatat acaaacatct agcatcagat agatggacag actgcatata
    55501 cggcttgaat ttcttgaccg tatcattcat caagagacag tgagatggca cagggacttt
    55561 cagcagcttc gctgtttgcg cttggtgccg tttggcttct gccaacgtaa cggataaacc
    55621 ttcgatactc ccagataacg tatgttgcat cggacaattt tcatttgaca gataaactgg
    55681 atggtcagga tcaaatgttt ttgctacaat ttcctcagct tcgcttctcg tcacacctac
    55741 tactacgccc attgcataac cgcttggata agcttctttc attaagactg cgcggtgata
    55801 gactagttcc gctgcattct caaatctcaa agatcgacaa gcaacagctg cacaaaaagc
    55861 accaatcgaa tgtcctgctg caatatcagg ctgaataccg tttttttcca tttcatctaa
    55921 ataaaatact gcttttgtca gtaatgctag ctggataaaa accgtatctt gatagttttc
    55981 ttctttatca actaaccgat accccgtcac attttctaaa cgttccatgt acatttcagg
    56041 gatatccgct aacatcccta acttctgact gccttgtcca ggaaataaga aacctgtact
    56101 catgagcctc gcctcctcag cacttacttt ttccactgct atcttagtca atttctgcta
    56161 taataaaaaa tgccaaaaaa agaaaaaacc ataacaaaaa ggtatggatg ggatggataa
    56221 ttatgaaatt gcttcatctg cagtatttta aaaaagtatt cgaaacaaaa aatgttaccc
    56281 aagcagccaa agaattattt atttctcaac ctgccttgag ccgtgcgatc aaacatttgg
    56341 aaaacgaatt aggtgtccct ttgttttacc atagcggcag aaacattgag acgaccgttt
    56401 atgcagaaga attttatcca tatgcagtca agacattaga cacattagaa gaaggaatca
    56461 aattattgtc ttcggtcaat gagaagatcg ttacttccgt catattgtat ttagaggtag
    56521 cctctgtttc tattccgaac ttggtccgaa tttttatgga aaagcatcct gatatccaat
    56581 taacgatcat gcaacatcat ttaccagatg atattaccga tcctgtactg tacatcactt
    56641 cggagatgaa gcctggcatg accaatattc ctatcatgaa agaacctgtt tatgtcgcca
    56701 ttcctaagaa acatcctctt gctaaaaaag aacatctgca attagaagat atccaacgca
    56761 cccccatctt gatgctgtca aagagcaacg ccttccgtca tacactggat gcagcacttg
    56821 aagcgaaaag tatcgaattg acgatcagtt cgacgacaga tgatcctgcg acattacgtt
    56881 ctattgtcaa gcaaggatta gctgttagct ttttcccaaa agtttcttgg tcttacgata
    56941 aaaatgatcc tttcgtcttg cgggaaatca aagatttgcc cttgacacga acaatttatt
    57001 tatcttctag cttcacagaa gatcatcctt tgatcaaaat gattgcaaaa acgatgcggg
    57061 aattttattt aggaaaataa aaaaaaaaga gctgggacaa aattcttgtc gcagctcttt
    57121 tttcgaacaa acggtgttcc agaagtaatt tcttagaaaa taagccgaaa tttcccaaaa
    57181 atttgagaag caattttcgt aaattccttc ttatttcttg aagctaaaca tttctgtcac
    57241 aacctcttct ctattgtcct agacattcat tgcgttgatt taaaacttcc atcaatcaaa
    57301 tctgctagtt catctagctt ttctatttta cgtagatcaa tagagatcca atgttcttta
    57361 ttcatgtgat aggctggaaa atacccctct tttcctcgca aaattccgcc aagttcagga
    57421 tcgaccttca aatcaagaat attgatgatc ctatggtcac ttgcatcatc atagattttt
    57481 tcttctggaa cagacatcag gaccccaaac cattttttgc ttttttgatg tctgaatacg
    57541 caataatcag gaaatttttt aaaaatatat tctggagaca aattgtatgt ttcattgatg
    57601 tgatcgatta gttctttttt cgttagcatc tgcttccatc tctttcttat tgattcgctt
    57661 tattcaaaga taccacgtaa gaatagaaaa atgaatacaa gtagttgcta atcaaatact
    57721 tcgatatttt tcttttttat caactcttaa atgttcaatc cgcctgtgtt ggtcttgcta
    57781 ccatctacta ttaaaatgtt tattattgtt tgcccaccta ttttttgatt ttagtggtat
    57841 catttttcaa gttcatgatg aaattcttat catcagtaaa tgctccagaa aaataaacaa
    57901 tgattgaaaa atcagttgaa acttgaccac cgtttatttt ctccgtattc ttagaataga
    57961 agggagtctg attgatgtat tcactaatga aaaaaatcat taccgaaaaa gatattcgac
    58021 gtcatgtttc tttagtagaa caattgctga atcatacaaa gataaccgta aatgaattag
    58081 ctgaaatcat agggacaacc gaacgaacga ttttttctga ccttcaatct attcgctctc
    58141 agctgcccga aggctgggac atcatcagcg atcaagcagg aatcagtttg caaaaccagc
    58201 aaaatttatt aacaaatgac ctttgggaga tttttttcaa acaatcagtc agtgtcgaac
    58261 tgctgaaaaa tctgcttttt acaaaaaaag ttgctgtccc tgattttcta gccgaccatg
    58321 ggctttctta tgggacattg aaaagacatg tgactaaaat caatcagcgt ttagcaagtt
    58381 acgacttgca gatcgatttg acgaaatata cagcttgtat tcttggaaaa gagcgtgcga
    58441 ttcgtacttt ttatcatcga ttgctgattc ctttcactca taacaactat tttttcgagg
    58501 attattcgat ccatgaatcc cattattttc aatttttaag aaacctcagt caaactgaac
    58561 tagcggttga aacagaagag attttcggaa catgttggtt ctttatcaat acgatccgta
    58621 taaaagcgaa ttgccgatta gattcttcta tccataccaa cagcacactc tcctccctgt
    58681 atgactctgc attgaaaaaa ttgtatttaa cggaaggtat ttatcttaaa gacactgagc
    58741 tttcttttgc ttctttctgc tttctggaaa gttggaatta caacaataac tatggacaag
    58801 aaaccgctcg ctgcctgcat cattctccat ttttagaggt cctcgaaacg tttgtagagg
    58861 aactagcttc agagctgtct cttgatcaac taaaaaaaac atctctgact gataacttgg
    58921 ctctcctcat attaaaatat catgaatcgc cgatattgat tgaagcattg gaccgtcaat
    58981 accatcgttt tttagaaaca tatgatcagc aatatgcaca cttgtatcaa cagaaagaga
    59041 atttactgaa cgatctaaaa aaagaaatag aaatcaatga accaaattac tttctccaac
    59101 tcttgtctct actgatccaa caaacgattt tttctatcaa accgaatttg ttcaatgtct
    59161 attttatttt ccaaggagaa ccttcatgga aggcgttctt gcagcaggaa ttaaaagatt
    59221 acttaggaaa acgtgtctgc ttcttaccta tcgaactaac cgatctttcc actatttctt
    59281 ttgaaaaaac agatatcttg atttccaatt tccctctaga ttctctagat atccctattg
    59341 tatacatttc ttctattcca acgaaaaatg agctcaatcg tttgactgaa ctcactttca
    59401 aaactttttt atagtaacgg ctagcgactg tattctctag ctgttttttc ttttttgcga
    59461 aacagtttgt tcatgatccc tttttctatt tctacaacta agaatagaag aatcacattt
    59521 gctccaatca ataactgttg tgtcatcgtt aaacttactg taccgatcag ctgctggccg
    59581 actggtagaa aaataaccat cccttgcaat aaagcaagga tacctaatga aagaaaaagc
    59641 gctttgtttt gcaaaagacc ttggttcaat gacggcttgg acaactctct gcaattgatc
    59701 atgtaaacag cttgagctaa gacgatattt tgcagaagaa tggtttgctg taagctttgc
    59761 ccttcaaact gcatagccaa taaataggca ggaaccatga tcagtaggga gacatagatg
    59821 atccgaaaca aatgataacc agataggatt ccttgctttg gatctcttgg tgggcgcttc
    59881 atgatttctg gatctggcga ctcgaaccct agtgcataag ataaagtgat tgtcgttacc
    59941 atattgaccc ataaaatctg tactggggac aaaggtagcg ggcgattcag cataagtgcc
    60001 caaatcaaga tcaatccttg agctaatgca gttggcaaga agaaattgat ggttttttgc
    60061 aaattatcca aaatccgccg gccctctttt acggcttttg caatcgtatg gaaattatca
    60121 tctcctaaaa ccatatcagc tgcctgtttg cttacttcac tacctttgat tcccatagca
    60181 attcctacat cggatctttt caaagcaggt gcatcattta cgccgtctcc ggtcattccg
    60241 acgatttctc catttttctg caaagcttta acaattcgca gtttatgttc tggtgtcgtt
    60301 cgagcaaaga catcgactat tggcacttgc tgaatcaact cttcatcaga cataagatcc
    60361 aaatcgatcc cttctaaaac tttggcagta tgttttaagc caatttgttc cgcaatcgct
    60421 tttgctgtgt ctttgtgatc ccctgtaatc attttcactg agatccctgc ttgttggcat
    60481 tcctttacag ctttgactgc actttctttt ggcggatcga tcaatccggc gatccctgca
    60541 aaagttaatt gggttaaagt ctcatgcgtg agttcctgtt tcgaatctac ttttttataa
    60601 gcaaatccta aaacacgctg accttttctc gcaaattctg cagctgtctg actccatgct
    60661 tcttgttctg cagaagaaag agttgattta gcaaataata cttctggtgc acctttgacg
    60721 taaatcaccg ctcctttttc ctccgcatgc atcgttgcca tatatttgta ttcagaactg
    60781 aacggaatct ttgcttctat ttttttcaaa ggtagattgg ctgtttctgt gtgtcgaatc
    60841 agcgcaactt ctgtcggatt gccacttaaa ttttctgttt tctgttcttc ttttgttttt
    60901 aattcctgac agtttttcat aatggacaac acgcaggctt cttcttttgc agctacatcc
    60961 acaactgtca tttcattttt tgtcaatgtt cctgtcttat ctgaacagat cactgtcata
    61021 gaacctaatg tttctaccga aggaagtcct ttgatgatcg ccttttcttt tgccatttca
    61081 tgtactccaa cggatagaat catggtcaaa acagctggca atccttcggg taccattgac
    61141 accaccaaag caatcgtagc cgaaagaagg atatgccaat ccataccata tcgaaatgtc
    61201 gtaaaaataa ctaagaatag gctcaaacaa agaatgcctt gaaaaatttg tttgtttaat
    61261 cggttgattt ttttaatcag aggcgtcgtt tgttgttcga ccgattgcaa tgcttgattg
    61321 attttaccga tttctgtcgt gtttcctgtt tctacaacga caccgactgc agaaccagat
    61381 tgtacaagcg ttcctgaaaa agccatattt ttctggtcgc cgatcgattc ttcttctgtc
    61441 aaaacggcat gacttttttc agatgcttct gattctcccg tcaaaatcga ttcttctact
    61501 aataaatcat gtgcttcgat taatcggata tctgctggaa cgacatctcc ggcacgtaag
    61561 acaatgacat ctccttttac cagttctttc gcagggattt tttcttgcat cccgtttgat
    61621 agtactatcg cttcttgtcc catcagctgc ttcaaaccat tcaatgattc ttccgctttt
    61681 ctttcttgcc agtaactaac gagtccattg acgatcacta cgaataaaat aatacttcct
    61741 tcaatatatt cactagaagc aaatttcaaa aaagaggcaa tgatcaacac gatcatcagc
    61801 agatcagtaa aatgctttgc tatttttttc catcgttttg tctgctgcct tgcttttatt
    61861 tcattatatc cttgatcagt caggcgtttc tgtcgttctt cttgcgacaa gccttctgtt
    61921 gatgtttgat agtttttcaa tacatcttct accgtcaatc tgtaccagtt catcctatcc
    61981 cctcctgttt aattgatact gttattatac gcaggaaagt tttttcaggc gtatcgatta
    62041 tttcaggggg gtctgaaata attcgttttg caaaatgtat ctgggcctaa ctgatatttc
    62101 tattgcttat tcttttatat acaaaattta agaatacaaa ccagataacc aataaaaaag
    62161 gaataaagcg tgtcgtgctt tcgaataaca agctgaaatc aattgaagta aaaaacacca
    62221 acaaagcaag atctttatat ctttgtgtag tgttccgttt cttttcttta ataaattgaa
    62281 catgtgcaca caagatcgaa gaccacacaa atagaaaaca actcgttgtc atgcctgcca
    62341 aaagctgaaa aaacaatcga ctatcagcta caaaaaaatg tagacaaaga ccggcaaaaa
    62401 ataacattcc tgtaattaaa ataccgtgta aaggtacgcc tttcttagaa agcgcacaaa
    62461 accaatgggg caaacttttt tcttttgctg tttgatataa aatcctgcta gtactgtaga
    62521 ctgcactatt tgctgaggac aaagaagcaa ccaaaatcat tagattgaca attgctgctc
    62581 cgccttggca atggatcact tgcagcatag tcactaaagg actttctttt aaatctaaac
    62641 aatgccatgg aacaacaaat aaaatcgaca aaatcgttcc tatatagaaa aagagaatac
    62701 gccaaggcag acagttgatt gcttttttca aattcttttg cggatcactt gtctcgccaa
    62761 cggttaagcc gatcatttca attccagcaa aactgaataa aaccatagga aatgagacta
    62821 ccattccagc aaagccaaac ggtagtaatt catcgatctg tccaaaattt tctttgattc
    62881 cgatagactg ggtatgaaaa tctgagacac tcaatcctat acccaataag ataaaaagga
    62941 aaatccccaa gatcttgatg attgagaaaa aagattccaa ttcaccaaac cacttgactg
    63001 caacaaggtt gactgccatt aagatcatta gtgcgctacc tccgcttatc aaagctggta
    63061 catgtacaaa ccagaaatgg atatacaatc ctattgctgt taattccgca agtccagctc
    63121 caagccagca aagccaataa gtccaattga caaaaaatgc ccactttttg cctaaataat
    63181 agtcaacgaa atcactgata gagttaaatg tactatttgc taaaaataaa tcacccatgg
    63241 cccgcatcaa accataaata gccaaaccgc atatcaaata aacgagaata acagctggcc
    63301 ctgctgatgc aattgccttt gctgaaccta aaaataatcc tgttccaatt gcgccaccta
    63361 atgcgattaa ctggatattt cgattagtta gagtccgttt cattgccggt ttttccatca
    63421 agttcacctt tttctttttc aagcagcatg gcgacttcct gttcccaaga aggtttcttt
    63481 attttttcaa tacagagtgg tactacaaac aaaatgacac tgatggctgt gactgcaatt
    63541 agattgatcc atgttccagc tttcatcgtc agtccgacag aaaatccgat tgccgcaaat
    63601 aaaacaataa cgacagtcca aactccaaga ttgctctttt tctctttttt tccaattcta
    63661 aatggtcgat tcaactttgc atgtgtataa cgcaatctca aaactgcgat ccccatgatg
    63721 acatagacga tacagtagat aacagtagtt gaatttacga gcatcaaaaa tgcttgattg
    63781 acgccaggaa tcagcaaata aaaaagtgca aagattgaga taatgacaga ctgagtcaaa
    63841 ataacggatt tggatacgtc aaattgattc gtcttccaaa aattgaattt tggtggatac
    63901 gcaccttttc tagcactttc tgtcactgtc tttgctggtc ctgaggccca agcagataat
    63961 tggactgcta caccaatgaa gacaagtaaa ctaaacagat tcacaataag atgtggcaaa
    64021 ccaagaattt ggcaataaat cgaaatagat tgcgcgatat tattcaattc catttgtcct
    64081 tttggtacga catttgctac aactaaagca ttcaaaatat tgacgaaaaa tataaaaatt
    64141 aacgcggcaa atactccgcg aatatacgtt ttaactggat tttccaatcg agtgatatac
    64201 acagaggaca tttcgatacc agtaaaaata aacatgatcg gcgcaaaata aacaaatgag
    64261 gatgcagtcg tagtatctgg tattaatttg tcccatgaaa aactgcctaa tgttccggtt
    64321 ggtacgatcc caactttgat ccacgcagca aaccctaata agagcatcat cgtcaaaggc
    64381 acgtacaatc ctaaccaaac accgactttt ccgccgattt ttgccatatc aaatttcaaa
    64441 ttcaaaaaag tgatgatcca ataaacgact aaaatgacaa ctaatgtaaa tttgctgttc
    64501 aaaccaagcg gcacattatc gataatatta ccaaacaacg gagcgagtgc tgaagcgacc
    64561 atcaccatcc caggaaacat ttggacccat aacagccatg agacaacgaa cccccatttt
    64621 tttcctagag cattcgatac ccaaagttct ggaccacctt tttggggaaa cattccagca
    64681 aattcaccag agatcaatga gattggaaaa gcgtacaata atactgcaac cagcatgtaa
    64741 aaaatcatcg tccagctcgt tgctgctaca tctggaatat ttcgtacact tgctacaagg
    64801 gcgcaggtca ttccgataaa ggtcatggtc gtgatttgct ttgatgaacc agtatctttc
    64861 atctttattc cttctttcaa tagcgacata tgtttttgtt ttcttttatt tttgttcaag
    64921 aacagaaatg acaaggtttc atcgattcag tacaaaaccc agtatcaccg aaacatccat
    64981 tgaacgatct tagtgaactg ggtttgatac atgaaacgaa cgatccaact tatagaacgt
    65041 tttataaaaa agaatatctt ggcgaggaat atatgaaaaa cagggggaaa tacaattgtt
    65101 ttgatgaagc aaataattgt ctgttctcca tatgtctctt gttattcttt cacacaaaaa
    65161 tcagttctat tggatgattt cgtttagttt cttttccatt gctttggcga ttttttttgt
    65221 ataaaaattg aaaattcgat catcattcaa ataaggagtc atgatagccg cacgtagcag
    65281 agtgattttc tgttcttttt tccattcttc tttcgttaga cccattcttt caacaaaacg
    65341 aatcggagaa tcaccgtaat cttcttgtgt aaatgtagta tgagaagtaa taaaccgttc
    65401 accataaaca tcgccatcca tataagaaga ccgatcgaac attttttcat ttaattcatt
    65461 gatagcgttc aaatctgtac agccttgttc tttgaatacc caattgacca tattaaaatc
    65521 aggatgattc aatggataga cttcgattgt ttttccttta acagtaaaag tcagatgatc
    65581 gagaaattct ctgaatcttt gagctgcctc gattgaagcc ccaatcaatt gaccatatcc
    65641 ggtcacattc aatggcaata cacgatgtgc tgtccagact gcggcggcgg ttgctccagc
    65701 ttttgatcct tccaaaatat aagctcctaa catatctgga gcctttactg atttttcaaa
    65761 cacatatggc gcgaaataag aaatgatatt tctcatattt ttatgtttga ttacgattcc
    65821 tccagcagca taagggacat agcccatctt atgaggatct atcgtgacag aatccgcttc
    65881 tgaaatagct ctgaaccctt catatacctc tttgtcaatc gttacgcagt ggtggaaaac
    65941 atgatgttct tcaaagaatt cagctaagga agcatacggc acaaattctc ctgcttcgtt
    66001 tagaaacagt gaacgcgcat agccgccata tgcggcatca acatgtaaat agaaatagat
    66061 cccttcatct tttaaccttt ctcttaattg gacgatttta tctacactgt ctacttgacc
    66121 ttcttctgtc gttcctacga cagcaaccac acctagaatt gggatttttt gtccagctaa
    66181 ttcacgaatt gttttttcca aagcattaat atccattcga taatcctctt gaactgggat
    66241 agcaaccatc tgatctaagc cgactccgca aatatctaac gccttcatcc aagaatagtg
    66301 ttttgtttga gggaccagcc atttccctaa tctttggata ttttttccgc tacgggaaga
    66361 agctgctttg acctcatcca tttcttcgtc cgtaaagctt tcggtcattt ccaaaatttc
    66421 ttcaacagat aaattcaaaa gttcccactc agacattttt ttcacttttt caggcagcac
    66481 ttccttgaca gctaaaggaa tcgatttgat gcaacgggcg taccataatc cttcaagatt
    66541 agcaatagaa ccatctgctg caatgtgtcc ccatccgtct gtcatattga ataatgaagc
    66601 aaaatcctgt cctacttctg cttccatttg cgaagttgcc atagaagatt ccaatgcaac
    66661 attatttggg ttccacagca tcgcatagtt gtaagctaat aaagcaggca tcaatgtttc
    66721 agcattcatt tgtccccagt aacggcctgc tgaatgccat gggatcgatc ctgcgcgtaa
    66781 tcgctgactg acttcatcca agacctctat catatggtcg cgggtggcta gataatctgg
    66841 agtgagctta tctccttcgc taattgcagg catatctgaa ggaatataat tttctctcca
    66901 tccaagatgt tcatctacca tcttgttcaa caacatttta taaactggtc cattttcagc
    66961 tttgtcgcct ataaagacgg ccttgatatc catatctttc atttttcttc cctccatatt
    67021 ttgttggtca atgtcttgca gatacatgtg tttatctgta acacctcttc actatagcgc
    67081 caaaaatgag aaattttttt ttataacatc aaatccacgt agatttttac aaacaaaaat
    67141 aaataaagtc ggatttttct gataattcaa cgcaaaaaag cgaacaattt ttcaataaat
    67201 cagtcttcta aaatagataa cctaaaaaaa gcgaacaaac aaataaaatg actgtccttc
    67261 attcactaaa catattttta tttttttat
//
LOCUS       DAFFSV010000002        64618 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFFSV010000002 DAFFSV010000000
VERSION     DAFFSV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.002"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="LR110_000060"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="LR110_000060"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBL4821067.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="LR110_000061"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="LR110_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBL4821068.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="LR110_000062"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="LR110_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBL4821069.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="LR110_000063"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="LR110_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBL4821070.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="LR110_000064"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="LR110_000064"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBL4821071.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="LR110_000065"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="LR110_000065"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBL4821072.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="LR110_000066"
     CDS             complement(8557..9027)
                     /locus_tag="LR110_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBL4821073.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="LR110_000067"
     CDS             complement(9073..9498)
                     /locus_tag="LR110_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBL4821074.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="LR110_000068"
     CDS             complement(9565..9834)
                     /locus_tag="LR110_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBL4821075.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="LR110_000069"
     CDS             complement(9845..11440)
                     /locus_tag="LR110_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL4821076.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="LR110_000070"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="LR110_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBL4821077.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="LR110_000071"
     CDS             complement(14993..15553)
                     /locus_tag="LR110_000071"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBL4821078.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="LR110_000072"
     CDS             15906..16880
                     /locus_tag="LR110_000072"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL4821079.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="LR110_000073"
     CDS             17050..18450
                     /locus_tag="LR110_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBL4821080.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="LR110_000074"
     CDS             complement(18528..19250)
                     /locus_tag="LR110_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4821081.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="LR110_000075"
     CDS             complement(19329..19835)
                     /locus_tag="LR110_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL4821082.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="LR110_000076"
     CDS             19958..21757
                     /locus_tag="LR110_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBL4821083.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21825..23399)
                     /locus_tag="LR110_000077"
     CDS             complement(21825..23399)
                     /locus_tag="LR110_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL4821084.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23654..24163)
                     /gene="mreD"
                     /locus_tag="LR110_000078"
     CDS             complement(23654..24163)
                     /gene="mreD"
                     /locus_tag="LR110_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBL4821085.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24167..25021)
                     /gene="mreC"
                     /locus_tag="LR110_000079"
     CDS             complement(24167..25021)
                     /gene="mreC"
                     /locus_tag="LR110_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBL4821086.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25176..25814)
                     /locus_tag="LR110_000080"
     CDS             complement(25176..25814)
                     /locus_tag="LR110_000080"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBL4821087.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25815..26465)
                     /locus_tag="LR110_000081"
     CDS             complement(25815..26465)
                     /locus_tag="LR110_000081"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL4821088.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26478..27377)
                     /gene="rsgA"
                     /locus_tag="LR110_000082"
     CDS             complement(26478..27377)
                     /gene="rsgA"
                     /locus_tag="LR110_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBL4821089.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27542..29611)
                     /gene="pknB"
                     /locus_tag="LR110_000083"
     CDS             complement(27542..29611)
                     /gene="pknB"
                     /locus_tag="LR110_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBL4821090.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29608..30348)
                     /locus_tag="LR110_000084"
     CDS             complement(29608..30348)
                     /locus_tag="LR110_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBL4821091.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30361..31719)
                     /gene="rsmB"
                     /locus_tag="LR110_000085"
     CDS             complement(30361..31719)
                     /gene="rsmB"
                     /locus_tag="LR110_000085"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBL4821092.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31719..32657)
                     /locus_tag="LR110_000086"
     CDS             complement(31719..32657)
                     /locus_tag="LR110_000086"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBL4821093.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32650..33141)
                     /gene="def"
                     /locus_tag="LR110_000087"
     CDS             complement(32650..33141)
                     /gene="def"
                     /locus_tag="LR110_000087"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL4821094.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(33156..35564)
                     /gene="priA"
                     /locus_tag="LR110_000088"
     CDS             complement(33156..35564)
                     /gene="priA"
                     /locus_tag="LR110_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBL4821095.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35817..36122)
                     /locus_tag="LR110_000089"
     CDS             complement(35817..36122)
                     /locus_tag="LR110_000089"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBL4821096.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(36127..36741)
                     /gene="gmk"
                     /locus_tag="LR110_000090"
     CDS             complement(36127..36741)
                     /gene="gmk"
                     /locus_tag="LR110_000090"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBL4821097.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(37228..37512)
                     /locus_tag="LR110_000091"
     CDS             complement(37228..37512)
                     /locus_tag="LR110_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821098.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37530..38420)
                     /locus_tag="LR110_000092"
     CDS             complement(37530..38420)
                     /locus_tag="LR110_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBL4821099.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38440..38658)
                     /locus_tag="LR110_000093"
     CDS             complement(38440..38658)
                     /locus_tag="LR110_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBL4821100.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38628..40328)
                     /locus_tag="LR110_000094"
     CDS             complement(38628..40328)
                     /locus_tag="LR110_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBL4821101.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40595..41368
                     /locus_tag="LR110_000095"
     CDS             40595..41368
                     /locus_tag="LR110_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821102.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(41449..42018)
                     /gene="thiT"
                     /locus_tag="LR110_000096"
     CDS             complement(41449..42018)
                     /gene="thiT"
                     /locus_tag="LR110_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBL4821103.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(42084..42186)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(42192..43163)
                     /locus_tag="LR110_000097"
     CDS             complement(42192..43163)
                     /locus_tag="LR110_000097"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL4821104.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(43257..44630)
                     /gene="glmU"
                     /locus_tag="LR110_000098"
     CDS             complement(43257..44630)
                     /gene="glmU"
                     /locus_tag="LR110_000098"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBL4821105.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(45133..45951)
                     /gene="purR"
                     /locus_tag="LR110_000099"
     CDS             complement(45133..45951)
                     /gene="purR"
                     /locus_tag="LR110_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBL4821106.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(46118..46942)
                     /locus_tag="LR110_000100"
     CDS             complement(46118..46942)
                     /locus_tag="LR110_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL4821107.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46915..47595)
                     /locus_tag="LR110_000101"
     CDS             complement(46915..47595)
                     /locus_tag="LR110_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL4821108.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47618..48556)
                     /locus_tag="LR110_000102"
     CDS             complement(47618..48556)
                     /locus_tag="LR110_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBL4821109.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48801..49664)
                     /locus_tag="LR110_000103"
     CDS             complement(48801..49664)
                     /locus_tag="LR110_000103"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBL4821110.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49806..52379)
                     /gene="mprF"
                     /locus_tag="LR110_000104"
     CDS             complement(49806..52379)
                     /gene="mprF"
                     /locus_tag="LR110_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL4821111.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52520..53326
                     /gene="proC"
                     /locus_tag="LR110_000105"
     CDS             52520..53326
                     /gene="proC"
                     /locus_tag="LR110_000105"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBL4821112.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(53405..54961)
                     /gene="rny"
                     /locus_tag="LR110_000106"
     CDS             complement(53405..54961)
                     /gene="rny"
                     /locus_tag="LR110_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBL4821113.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(55372..56421)
                     /gene="recA"
                     /locus_tag="LR110_000107"
     CDS             complement(55372..56421)
                     /gene="recA"
                     /locus_tag="LR110_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBL4821114.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56530..57771)
                     /locus_tag="LR110_000108"
     CDS             complement(56530..57771)
                     /locus_tag="LR110_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBL4821115.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(58011..58589)
                     /gene="pgsA"
                     /locus_tag="LR110_000109"
     CDS             complement(58011..58589)
                     /gene="pgsA"
                     /locus_tag="LR110_000109"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBL4821116.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58798..59721)
                     /locus_tag="LR110_000110"
     CDS             complement(58798..59721)
                     /locus_tag="LR110_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4821117.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(60460..61746)
                     /locus_tag="LR110_000111"
     CDS             complement(60460..61746)
                     /locus_tag="LR110_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL4821118.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61739..63007)
                     /locus_tag="LR110_000112"
     CDS             complement(61739..63007)
                     /locus_tag="LR110_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL4821119.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            63259..63720
                     /gene="mscL"
                     /locus_tag="LR110_000113"
     CDS             63259..63720
                     /gene="mscL"
                     /locus_tag="LR110_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBL4821120.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63779..64225)
                     /locus_tag="LR110_000114"
     CDS             complement(63779..64225)
                     /locus_tag="LR110_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4821121.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(64318..64563)
                     /locus_tag="LR110_000115"
     CDS             complement(64318..64563)
                     /locus_tag="LR110_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBL4821122.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT        17846 a        14011 c        11547 g        21214 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgattaatc attcttacat gctgacagca
    21841 aagtcagggg caaaccattg aactgaacca acttttacgc tttcgcctgg ttgaggagca
    21901 tggatatatt gtcctccgcc taaagcaatc gctacgtggt aagttccgcc ttgtgacccc
    21961 cagaataaca agtctccagc ttttgcttga gaaactgaaa tttttgttcc agctgattct
    22021 tgaggaactg tccaaccacc gatgtcacga ccagttactt gcatgtaaac atagcgtgtg
    22081 aaccctgagc agtcaaagcc acttggatct ttaccgcccc aaacatatgg agtaccaata
    22141 tatttgtaag cttctgctac gattgctgca ccattcacac ttccagaagg agctggagca
    22201 ggtgttacag gttttggttg ttcaggtgtt ggtgttggcg ttggagccgg agtactactt
    22261 cctgtgccgt ttcctgtatc aacactttga tctgttgatg gtgttgtagg cgcaggtgtt
    22321 gttgattctt ctgttgttga agattcaggc actgttgttg attcttctgt tgctgaagat
    22381 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcgctgttgt tgattcttct
    22441 gttgctgaag attcaggcgc tgttgattct tctgttgaag attcaggtgt tgttgattct
    22501 tctgttgctg aagattgtgt agctgatgat gattcttcag tcattgaaga ttgtgtagct
    22561 gatgattctt ctgttgctga gctttgtgca gtagatgaag cttgagtagc ttgtgcttca
    22621 gcctctgctt gttcgcgtgc ttctttttca gctttttctt gtgcagcttg ttgacgagct
    22681 tgttctgcta gacgttgttg ttcacgaata cgagcttgct ctgcttcagc ttctgctttt
    22741 tgacggttca agtcagcttt cttgtcttca gcagttgctt gttcagcagc taatgaagtt
    22801 ttcagtacgt tcaaatcagc ttgtttagat aaaagatcgc ctttttgaga ttccaaagca
    22861 gcttggtttt cagctaattc ttttagttta gcatcgtttt cagctttttt gtcttctaca
    22921 gcttttttat cttgtttttg ttgttccatc aagtcgttgt ttgcttttac catcgttgtc
    22981 attgcttgaa cgcgtccaat agcatctgct aatgaatcag cattcaatac agcatcgatg
    23041 tagtttgagc ttgtgttgct tacttgtgct tcgcgtgctt gtttttggat tgtatcttca
    23101 cgtttttcaa tacgttcttg taaatctgca atgtctttta ctaattgtgc agattcttga
    23161 cgcaatgtat cttgttttgc taataggtct tgtgcttgtg tgttgatttc agaaacttgg
    23221 ctttctaagg catcgatttg tgattgtgca tctgcttgtt gatttttcaa gtctgcaatc
    23281 ttttgatctt gctgttgtat ctgagaatca aaatcgtccg ccgcggcagc gatcggtgat
    23341 gctacggcag tcaaggtcat tgaacatacc attactgctg atattaaact ctttttcact
    23401 cttcattcct ccgactggct taatttaata aatatctaaa tatatttaag tctttcttaa
    23461 cgatataaaa attgtaccat agcagtgtgt caacgatata atagttatgt tacaaaaatg
    23521 tttcaaaaca acttttaaaa atgacaaaac tgttcgtttc agaaaatgat catgaaaaag
    23581 ccgcgttaag tctagcattt cttaagataa caacgattct ttttcgcttt cgacctaaaa
    23641 ataaaaaaag aaattattca tcactgaata atttcttaaa gggaaaaatg aataatacaa
    23701 atataatcat gttcaacagc agtgtaggtc ctaaaaacct tgtaataaaa tacgtgttat
    23761 ttactactgc taatttaaaa atcaactgga ccaccatcgt aaacaattca taacccgtga
    23821 caaagatgat catactgaaa aattcagtaa agatgttgac atggataacg tctttcatta
    23881 catacatcaa ccatacaatt aaagggagag ctactgcata gataccgata acgccaatat
    23941 agtaagcgtc atagatagcc ccgagaacaa tcgtggtaat cagtaaataa cgtttttcaa
    24001 acgccatact gcaacataat aatgccaata tcagaaagtg ggcatttgac atataggtgc
    24061 ctttcgacca ctcccctagc attcttgtta aatgaccatc tatcaacatc aaaaagaaca
    24121 aaacgattgg cagatagtac ttcattgttt ctttcttcaa catggactaa tcctccgcta
    24181 gccgttggac aatcgtcaca actgacacgt catacatttc tgcataaggt ttcacataaa
    24241 cttcccgatc cagcccataa ctatctggtt tcgttttaat aaccgtaccg atcggcaagt
    24301 tagctggaga attccctcca agaccggatg tctggacaac atccccttct ttgatatccg
    24361 tatcaccagt taattgggtc accactaagg catggagctt ttcatcatag tttttaagca
    24421 aaccaaacgc ttcgccatta gccgaagata cccgtactgg aaaatgattg gagctttcat
    24481 tagaggatga cagtaattcg attttagacg aagccgtatt gacctcgatc actcggccaa
    24541 tcaatccttt ttgcgacatg actgccatat ttgcttcgat accgtcttta gaacccttgt
    24601 ctacgatcag catgtcctgc caactgtcag gcgaacgagt aatgacattt gccgttactt
    24661 tttcgtaact agtcagcgtc tgattcaact caagttcttt tttcaacgct tcgatttctc
    24721 tctcgtaatt cttgttttgc tgtgaaacgt catcatagct atcgattttt tcttttagcc
    24781 gctcattttc ggcataagtt gaaaaaagat tatggacaga atctactcca tttcgtaccc
    24841 accgtactgg ggcagagatt gttttatcaa ccattgcaac actgtcatta acgatggatt
    24901 ggaagaaatt gggctttcct tcattcgctc gctgcgcagc ggtcacactt agtatcgtca
    24961 ccacgatgat aacaataacc aaggtaataa taatattttt attaggattg aacttcttca
    25021 caaagacacc tcgaattaat tagactcttg tgttcaattt tatcactaaa tgccgtgaaa
    25081 caaaaggtaa gttgctcctc ttaagctttt ttttagcaaa taatagattc tgtcattttt
    25141 agcctgaaag acaaattttt ttatttgttt atggatcaat ctttgctttg aataactgca
    25201 atcataccat ctgaaaaaga aaattctgcg gtatctgaaa gaaattcgtt acttgcataa
    25261 gatgtcggat attctacttc tttattttcc aatacatact tgctgccttt taatgtaaga
    25321 ttcgagacgg gagtcaagca gcaataagct aaatacttca tatctggtat ctgtcttact
    25381 atatgctctc caggcaaata ataagacaaa taattttgac aatctctgat aataagctga
    25441 tgggcaaagg gtttgaagcg aggttccata cctaaccaga gattggctaa aagatgatcc
    25501 aatcttccgc ccgtagctcc aatcaaagtg acttccgctt gcgggtaacg ttcaaatgtt
    25561 tttaagatac ctaactgtgt atctgtatcg tctttttctg caggggaacg ggtatactct
    25621 ttggcttttg caaatacttc cttctgctct tcgttcgaca atgaatcaaa atcacccact
    25681 gccagatcta ctgggtagcc tgcttgaagc aggtacagcg caccacggtc tatcccgaca
    25741 taggcatcgt atttggacag cagagactgc ggccagtttt gcggattacc tccagcagct
    25801 aataaaattt tcatttagtc taatgcctct tttaatcgtt cgatttgtcc gactggattt
    25861 tcggctccat agatatatga tcctgcaacg aagacatccg cccctgcatc tttacagatt
    25921 tttgctgttt ctggaacgat tccgccatct acttcgattg cataagtcca attgttctct
    25981 ctacgtagat cagcgagttc cgtaattttt tctactgttt cttcaataaa gctttgtccg
    26041 ccgaacccag gattgacagt catgactagt acttgatcag ccaaaggcaa aacatgtttg
    26101 atcatggaga ctggtgttcc tggattgata actactccag ctttaactcc taagcttttt
    26161 gtcatttgaa gtgcccgatg aatatgagga gttgcttcga cgtggacagt aatgatatct
    26221 gctccagctt ttgcaaatgc tggaatgtag ttttctggat caacgatcat cagatggacg
    26281 tctaacggta atttagtaac aggacggatt gcctgaacaa tatttggccc taatgtgata
    26341 tttgggacaa atcggccatc cattacatct acgtggatgt aatctgcgcc tcctttttcc
    26401 actaactcaa tgtctctttg taaattggca aaatctgcac tcaaaatgga tggtgctatc
    26461 ttcattcttg tttcctccta ttttcgttta ccttcttttt ttgcatacat tggttttctt
    26521 ttttcgattt cttgtaagaa ttgcaaataa ttgtcgtaac gtgtctgtgc gataccacct
    26581 gtttctactc tatgttttac ttcgcagcca ggttctttag catgcatgca ctctctaaat
    26641 ttgcaatgga cagaagcttc tacaaattca ggaaattgtc tcggcagttc tcctgcttca
    26701 attgtcaaaa agtcaatcga gctaaatcct ggtgtatctg ctactaaccc gccatagata
    26761 ggaatcagtt ccacatgacg tgttgtatgc cgtcctcttc caagtgcatc agaaatctct
    26821 cctgttgcta aattcagttc cggtgatatt tgattcagta gcgtcgattt tcctgctcca
    26881 gactgaccca taaagaccga tagccgttct gggaagtaac ggatcagttc tgtcgtatca
    26941 cccgtatgat tgggtacaat caccggatag cctattttag tataggtttg cttgatctct
    27001 tcgatttttt ctgggtcgtc tgacaagtcg gtctttgtca aaaaaataat cggttcgatc
    27061 gactcgtatt ccaaggtaac tagaaagcgg tcaagtagat tataagaaaa tgcaggctct
    27121 acgatacttg tgacaatgat tccaagatca acatttgtga ctggtgggcg aactaattga
    27181 ttttttctcg gcaagatttc cagaagatag ccgtctgttt gattttcact ctcaaagatc
    27241 acctgatctc cgacaagcgg cgtgattttc cgattccgga aatttcctct tcctcttgtt
    27301 tgataggtct ctccgtttgc gtatacatag taaaaaccgc tgattgcttt tctgatttgt
    27361 cctttgataa gtgccacctc cgtttttgtt tattttacca tacgaagagg aaagtaggtg
    27421 ttattccatg caaacttctc aaagtatatg gcttgaaaat tgatcaaaag agatagaaac
    27481 acaaaaaaga gaatgcaaca aatcaatgtc gcattccctt ctgatatatt ttattcagca
    27541 cttattttga actttctgag gtgctagctg atgaacttgt ggagctggaa gattcttctt
    27601 ttgaagagtt gcttgtgcta ctggagctac ttgagctgct agaactgtct gaagagtctt
    27661 tttcagaact agaagacgct ggatctggtc ctttagagta aattacgttc acaaccattc
    27721 ctttggtaat tgtcgttcct gcccctggta ctgtacgaat cactttatct ttatccaccg
    27781 tgtttgaata ttcggaagtc tcatgcccga tatattctgc accgacacta gctaaataac
    27841 tttgtgcttc gctttttgta tagccggaga tatcattcaa tttaggctct gttccttggc
    27901 ttacgacaaa attgatgtaa gtttcttctg ctacaacttc acccgaggct ggatcctgac
    27961 tgatgatcga acctgatggt acagtgtcgc tatagtcata agtagcacta atattattga
    28021 tattgaatcc ttgtgtaaca agccaattac gaacagtttc gtagttttct cctacatgac
    28081 tactcaaagt cactgtttct ggtcctttgc tgactgtgaa agtaatcgtg tctgttttag
    28141 gatcgacttc tgtatcttct gatggagatt gcgaaatgat cattccgctt tctacctctg
    28201 aactgaattc ctcttctttt tgaatcaagt cagcagaaaa ccctaattct ttcaaacttg
    28261 cttcggcatc ttcataactt ttaccactgt aatcatctaa tttgattttt ttcgtgccgc
    28321 tactaatata cagagtgact tcgcgttttt gcttgactgt cgttcccgct gccgggtctg
    28381 tcttcactac attaccttct tcgattttgt catctgcaat ttcttctgtt tttgttgcta
    28441 cttctaattt cgcttttgcg agtgtttctc tagcagaagc ttcagaaaga tcagaaacat
    28501 caggaacaac aacttctcct cgcccgccag aagcaaagaa tacagctgtt ccgattccta
    28561 aaacagcaag aatagctaat aggatcagcc accatttttt ctgtcctttc tttttaggat
    28621 caggcagaac ttcttcctcc tctggtaagg tttcgttttc tggcttgtct tttggcagag
    28681 gcatagattt gaatgaatca ggcattggtg catcttccgt aaggggtgtg ataacttttg
    28741 tttcattatc catcacatga ggctgccatt tttcttcgtt tgcacgagct ggtgataata
    28801 ctgtagaaag atcatcagac atttcctcgg ctgacttata acggtctgca ggttctttag
    28861 ccgttgcccg taaaatcacg ttttccagtg cttgtggcac atttggatca agtgctttta
    28921 tagatggaag atcatcttga aaatgtttta aagcaattgt tacagcagat tcaccatcaa
    28981 acggtacgct acccgtcaac atctcataaa ggatgatccc taaagcataa atatctgact
    29041 gcttcgttgc catacttcct ctggcctgct caggggacag ataatgaaca gatcctagca
    29101 tcgtattcgt ctgcgtgatc gatgtttcag ataaagcgat agcaatcccg aaatccgtga
    29161 tttttacaac accttcatta tcgatcagta cgttttgggg tttcaaatct cggtggatga
    29221 tttgatggtt atgagctaaa gaaatagcag aaaggatctg ctgcataata tttactgctg
    29281 tttcataagg aacaggataa tgtgtttgga tatatcgttt caagtccatt cctttgacgt
    29341 attccatcac cagatactgc atgttgtctt cttcaccaac atcgtaaaca ctaacaatgt
    29401 ttggatgaac gagttctgtt gctgctaaag cttctctctg aaatcgtcga atcgcagtct
    29461 ggtcattttg aaagtcaaat cgtaagactt taactgctac atcacgatcc aagattaaat
    29521 cgtgcgccaa aaatacattt gccataccac cgctaccgat atttccagtg atttgataac
    29581 ggccatttaa ttttttgccc aactcgatca tacactgtcc tccttatatt cgataagaag
    29641 aacggtgata ttatccgctc cgcctgcttc attggcacga tcaatcagct cagttacttt
    29701 atgagacaat gggccttctt gattgatgat cgttcctatt tcttcttcag acagcatgtt
    29761 cgtcaagcca tctgaacata acagcagtcg atcttttagt tgccaaagat gagtagaaac
    29821 atctacttcg actgttccag gcatgccgac agaacgtgtt aaaatatttt tccttggatg
    29881 gttcactgcc atttcttcgc tgatttctcc tgatttgact agttcattga ctaaagaatg
    29941 atcctccgtc aattgcatga ttttatcttc acgaatcaaa taagctctgc tatccccaac
    30001 attggcaatc gtgaaccagt catctgacag agcggccgct acgattgttg ttcccatacc
    30061 gttatattcc ggctgttctt gtccacgctg ataaatcagc atattttctt cttgaatcga
    30121 ttgaatgaac cattgggcga ttttttcatc gtcaacagct tcttgttttt cccaagcttc
    30181 gcctagattc gttaccgcca tctgactggc aatgtctccg gcacgatgac cacccatccc
    30241 gtctgctaat acagcaagct tgagacctga acggctggta aatacactgg catagtcttg
    30301 atttgtgtta cgtcgtcttc ctacatctga ttgatattca atctgcattt tttcacctca
    30361 ttactttttg cgcaaacaac aaatgaaaaa tccatctgtg taaaactgat gcggatacaa
    30421 agtcaacatt ttctcttcga ttgatgattg tagcatagga ttggtcaaaa catcaatcct
    30481 ctcaaaatca gggtgtcttt ccaagaaagc agaaacaacg tcttggtttt cttcttttaa
    30541 gatcgtacaa gtgctataag tcagaatacc ggaagacttt aaagttggtg cgcagctttc
    30601 taaaatttcc aattggattt ttggtaaatt cgctaattca tccggctgtt ttttataacg
    30661 aatatctggt tttcgccgaa gcagcccaag acctgaacaa ggcgcgtcta ctaaaatccg
    30721 atcaaactgc tctgcttcga actgtttact gacttctcta gcatccatct tttgagcgta
    30781 gacagcagaa gagacaccta gacgtgctgc attatcttca atgagtttga ctttgtgttc
    30841 gtgtacatct aaagcgacca cttctccacc gcatgacgga tctaaaaaag aagcaatatg
    30901 cgtcgtctta cctccgggtg ctgcacaggc atctaagaca tgatgttcag gctcgatttg
    30961 catagacggc gctacaagca tcgaactttc atcttggatc gtcatgacgc catcgtggaa
    31021 caatgagcta gaagctaaat gccctttttt cgctactata ccgtatggac tgacttggct
    31081 tttttcagct gtgatctctt cttcttgcaa aacagtgatg gcctgatctc tggaaagtcg
    31141 gcgtgtatct actcgggcac tagcatggct aggctgatat agcgataaac cgagcttttc
    31201 agtttcttcg tatccgattt gatccaccag tcgtttcgtc atccatagag gcaggctgat
    31261 tttgacagaa agtctttcga taggatcagc aatgccttgc agatctggtg ctccttgacg
    31321 ctgataattc ctcaagacac catttacaaa tttccctgtc ccaggatttc cttttacttt
    31381 tgcgatttcg accgcctcgt ttaaaatagc atgtgcaggg actttatcta aaaattctaa
    31441 ttggtataca gaaagataaa gaagcatctt gacccagtca tccacttttt ttgctttagc
    31501 gatcaatggt gcaacgtaaa attccaatag tcgctgatgg ctaatcgtac catagatcaa
    31561 ttctgtaaat aaacccacat ctttttgatt tagctcgctt tgtttgatca tctcattcaa
    31621 caaaagattc gagtaggcgc cacccttgtt cactcgctct aatgtcagta atgccatata
    31681 tcgtactgat tttttcattt ttttagggat ttttttcatt atttcacctg ctgaccaatc
    31741 tgaacttgct gaccgcttcc gttaaggaaa tcatgtacgg cctgttttcc tttaccagct
    31801 ggctgtaatt cattgatggc taagacggtc tgtttaccgc aagcaatcca tagatttttt
    31861 ttgtctttcc ggatgatcgt accaggtgct ttgtctgtag tttctgccaa tacttcaacc
    31921 gataataatt tccagcgtat gccctcatag gtcgtgaatg caattggcca aggacgcatt
    31981 ccgcgtactt ggttgtcaat ttcttctgca gttttatgcc aatcgattgc ttcttgttcc
    32041 cgtgtgatgt ttggcgaaaa agttgcttct gactcgtctt gaggtactgg tttttgtcca
    32101 tccaaaatat tgggcaatgt ttctagcaat agctttcttc ctaaaaggct aagcttttca
    32161 aacatcgtcc ctacatcatc ttgtttagtg attggtatgc tttcttgcgc atagattccg
    32221 cctgcatcca tttttttgat catttccatg atcgttacac cagtttcttt ctccccattc
    32281 atgatggcat aatgaacagg cgcacctccc cgatatttag gaagtaaaga agcatggaca
    32341 ttgattgccc ctaattttgg tacttgcaat aacttttcag gcaagaattg gccaaatgcc
    32401 gcagtgacga tcacatcagg caccaattca atgatttctt ccatttctgg cgaaccggag
    32461 attttctctg gctgcagcac acggataccg tgcttcagag ccgctttttt taccggtgtc
    32521 ggcgtaatta ctcgtttccg cccaactgga cggtcgggct gtgttaccac tgcctggatc
    32581 tcgtaacctg attctatcag cccttcaaga atcggcacag aaaaagcagg tgtgcccata
    32641 aatactagct tagtcatcaa gatgttcctc catatattct tcaagttctt cttccgggat
    32701 ctgctgtatc attttctcaa tgaacaagat accgtctaag tgatcgattt catgctggaa
    32761 ggcacgggct aaatagccaa aagccgtcac ttctatctct tctccgtctc gatcataata
    32821 acgcacggtc acttcatctg ctcgcttgac cgttccatag acatgcggaa tactgagaca
    32881 accttctacg tcaaggcttt caccttttgc ttcgatgatt tctgggttaa tcagttcaaa
    32941 tttctcgcct tcatctactt cgatcacagc aattctcttg ttttgtccta cttgaggagc
    33001 agcgatccct attccgtcat tcgcaatcat tgtctcatat aaattgtcta ataaagcgat
    33061 tgtctcgtcc gtgattacat cgattggctg agcagttctt tttaatttat cattagggtg
    33121 gatcaatata ggatagcgca taatttggcc ctttcttaaa taaagtttaa tggttcgttg
    33181 tcaattgcca caaaaagacc ttgtcgctgt tctttctggc ttccattcaa aatttcatcc
    33241 aaaacatgag gaagattcgg ttcatgttta tatttgatga ttagttgata ataatatcgg
    33301 tttttcactc tcaaaatcat gctgggcgtt ggccctaata gcaagctttg agggcttaac
    33361 acttctttta gctgattggc aatctgaaag attttctttg ccgctacttg ttcttcagga
    33421 tggctggcgg ttattttcac agtaaagtaa taaggaggat agcctccacg atgccgtaca
    33481 tgcatttcct gctgataaaa ctgctcataa ttttgttttt tagccaattc tatggcgtaa
    33541 tgttgaggat tgaatgtttg gatcacgact tcgcctgctt tttccgctcg ccctgctcgt
    33601 cctgaaactt gggtaagcaa ttggaatgta tgctcactag aacgaaaatc cggtaaattc
    33661 agggaggtat cagcattcaa caccccaact aatgtcactt ctggaaagtc caaaccttta
    33721 gcaatcattt gtgtccctaa taatatatct gcttcttttg caccgaattt ttgtaaaatc
    33781 tgttcgtgtg ccccttttct tcgagtggta tctacatcca tacgcaaaat cctagcttgc
    33841 ggataaagtt ctcggagttc ctcttctact ttttgagtac ctgttccata gtagcggatt
    33901 ttattgccgc cacaattagg acagcgattg ggaataggtt cttcatgtcc gcaataatgg
    33961 cagcgcattg aacgtgtatc catatgcagt gtaagagaga tatcgcaatt aggacatgga
    34021 agaacaaatc cacagtccct acacatcaca aaagacgaat atcctcgacg attgagcagt
    34081 aggacagttt gttctttttt gtctaatcga ttttgtattt tttcctgcaa attagcagaa
    34141 aaagtagagg tccgatgatt ctgaaactct tcacgcatat ctacgatctc aatagctgga
    34201 agctgtgcag ctgctttggc tcggtggttt aactgcaata actgataaac gcctttttga
    34261 gctcgtgctc ttgattctaa agagggcgtc gcgcttccta gaactattgg gcaatgatga
    34321 tatttactgc gccaaattgc tagatctctc gcatgataac gcggtgtctc atcttgtttg
    34381 taacttgatt catgttcttc atcgataatg atcagcccga tatttttcag cggggcaaaa
    34441 atagcagatc gtgcgcctac tactacttgc gcttcttccc gctcaatctt gcgccattca
    34501 tcatattttt caccttggga aagtccgcta tgtaagaccg caactgattc tccgaagcgt
    34561 cctttgaatc gctcgaccat ttgcggagtc aatgcgattt caggcactaa cataatagcc
    34621 gttttatttt ctgacaaaac atcagcgatt gcctgaagat atacttctgt tttcccgctg
    34681 ccagtgattc cctccagcaa aaagacctga tctttttgct gctgacctgc tgtgatgatt
    34741 tgttcgacag catttttttg ttctgcattc agttccaatg ccgtcgtttg gtcaaatact
    34801 cggtctttat aagggtcgcg ataacgttct gattctataa actccagcca gcccttttct
    34861 tttccttgat tcaagacagc agtactgatt cctttatttt tgaaataagc aattggttct
    34921 tcttggctca gttcagataa acacaaaaga agttcttttt gtttcttggc attttgccgt
    34981 aattttgccc attcttcttc gatttgcttt tcagttaaca aagaacggac caaacgaatc
    35041 gtttttactt tatttcgtgt atggacttca tatttgatat ctactaattg ctcttggcgc
    35101 cagcgaatca attgagaaag gctgccttct tcttctgctt ccttccagga aatcacttct
    35161 cgctgatcaa acaatacctg aacttctggt gtgtctgcca aaggatagat caatttatca
    35221 tattctgctt tcatcacact cggaagcatc gtttgtagac aagtgatttt gaatgcgtaa
    35281 gtaatttttt tcatataatc agctaatgaa agtaattctt cattgaccac cggagcaagg
    35341 tctagcaaac gaataactgc cttcaaagat ggattagctg attcttctga tttttggata
    35401 gccataacaa atccttggac atgacgattg ccattaccaa aagggacttc tactcgcatc
    35461 ccgacttgca aagctgtttc catttcagag ggaaccaaat aagtgaatgg ctgatcggtc
    35521 tgcatagtag gtacgtccac aatgacttca gctgttaatg tcatggttcg tttcctcctt
    35581 tttttgtctt ttcttatcaa gcgacaaaaa gttccttgcc tattttacta gagatcgatg
    35641 gatttgtctt atcttctgcc aaatcctaac aaaaaattaa cagcagataa gaaaaaaaag
    35701 aactgtgtca aaaagttcag acacagcctt ttctaccaga caataagtgg cgaatgtttg
    35761 aaaaggaaac agctgggttt gcttttcttt ttagatgttc gctctatttt tctttattac
    35821 attttttgct cttcacggat acgtgattcc aattcgtgtt gttcacgctc tttttgtgct
    35881 tgacgttcct cttcttccat cttcaaacgt tcacgtttta attcaggatg tggatcattg
    35941 actacatttc ctgcatcgat ttcttctaaa gcttgaccga cgctttttac tgaatcaaaa
    36001 gaatctagag taggctgtgc acttgcatcc aattcatgcg cgcgtttact tgctaaaatc
    36061 actagagaat attttgaatt tacacgatct aatagtgagt caattgaagg ttttaacatc
    36121 ataagactac atctccttta acattttaat atatttaccg attacgcgat ctactcgaaa
    36181 atgttcactg gcaataatgt ttttgattcg ttcaactgct agcggcactt catcatttac
    36241 aacagcatag tcgtaaagag ccatcatttc gatttcttct tttgcaactt tcattcgttt
    36301 ttcgattact tcatctgcat ctgttcctcg gcctacgatc cgtgatttta attccgctaa
    36361 atctggcgga gtcaaaaaga tgaatacacc atcgggcact ttttctttga cttgttttgc
    36421 tccttggact tcaatctcca agaaaacatc ttttccttca tccaatgtct tgttgacata
    36481 tgacaaagga gttccataat aattcccgac atattctgcg tattccagca tctctccagc
    36541 ttcgatcatt gattcaaact cttctttgct tcggaagaaa taatcaacgc cttcgacctc
    36601 cccttcacgc attttacggg tagtcattga gatcgaatac tgaaaatcat tatcctcact
    36661 ctcaaaaatt gcttttcgta ctgtgccttt tcctacaccg gatggaccag acaacacgat
    36721 taataatcca cgctctgaca tgacgacgtc ctttcaacta gtctttttgt acttatcgtt
    36781 tattttgcac tttttttctg caactttcaa ggttatcttt aattatagct agaaattggt
    36841 aaaaaagcta ccctgtacca agaggtcgta aaaaagctgt cctgcatcaa gtaataagaa
    36901 cagccaaaca taaacagctt ctcatgtttt tcgtttggtt ggggttatta tcgcagatgc
    36961 aagcttttga acgccgtgta taagaggtcg tgaagaagcc gattagtttc gagtatgaag
    37021 aaaaattttg tgaaaatagt ttctcacatt tttgcataaa tttggcttaa taccgagaaa
    37081 atggtttttg aacatcactt attcaaaata aaaaaagatt gtgacacgaa ttttgtcaca
    37141 acctttaaat gtattcctag agcaaagaag ctaagcgaag aaatccaatt ctgtttattt
    37201 attgtatttc tttgttcagc atccttgcta aaagcccata ttggacaaag tattcactac
    37261 acccatgatc tcatcttttc gagaacgtgc tttttctgtc ggatcttcta cctcttctgt
    37321 gtcaattcga acaataaatt tccgattcat cgtcacgtaa gcaccgtcgt tgatcaaaat
    37381 aaacagttcg ttctcattat agtctgcaca ggcatctttc catacgtcaa agccgttttt
    37441 ttcagaaggg atatcccgtt taaccacagt ttttccactt aattcgtgga acgttacgtc
    37501 ataatatttc attatctcta cctctttcgt cattcaatat tttgtacttg ttctctgatt
    37561 ttttctaaga tcgtcttaga ctggacgact tgatttttaa tttcgattgc atttgatttg
    37621 gaaccgatcg tgttgatttc ccgattcatt tcttggatca ggaaatctaa ttcccgacct
    37681 acaggacatt gtgcttcgat aagttcatct agtttttgta catggatcgc cagacgatcc
    37741 agttcctcgt gaatatctcc gcgttccaac aagatggcta gctccgtcaa caaccgttgc
    37801 tcatcgactt tcgctccaag aaattcctcc agcttttttt gataccttat ttgaaattcc
    37861 ttttcataga tttctacaaa actatcgagt ttttttagta cttctttgaa ttcttcagta
    37921 ttctttttca acactaaagc taaagcttga ccttcttttt ctcgactcgc ctcaatagaa
    37981 tcaagtgcgc ttttcaatgt atctagagct aactgtgcca attcttccat gctctcttcc
    38041 ggtttttctt ctaccgttac gaagttttcg tccagcacga agcgttccgc catccgacta
    38101 attgacactt gattttgact accgtatcgt tgttgaattc cgtctgtcaa ttgagtgata
    38161 agtgaatcaa tcaattccca gttgatgacg atctgttttt cgtttttgcc aatataagct
    38221 agattcacaa atacttcgat tcgaccgcgg cttaactgct gtttaataag ctgacgaaaa
    38281 tcgttttcaa caaagttcag gattttagga cttcgtattt gaacatctag aaaacgctgg
    38341 ttgacacttt ttatttcaat atctaactga tacgcttttg tttcgcggat ggctttgcca
    38401 aaaccagtca tacttttcat tcgacagggt ccttcctcat tacatatttt ctattgcttt
    38461 ttgcagttgt tcaaactctt cgttcgtcaa tgtcaggcct ttccccattt tttcatggtc
    38521 tggcgcccaa tctcgaagat cgaattttgg cgggcgacca ttccagctga ctaggttcag
    38581 ttctttacgc caccccttgt tgttttctga caatatagcg atttcttcta cgatttcata
    38641 tgaaaattct tgtgccatgg ttgttcctcc ttgagataat ataaatatag gatcagtaat
    38701 gtgtcaactg gttgcttaag caattcatgc tgcaaacgat ccttgcgttt tccttgtagc
    38761 agataatcaa aataaacagt tttcgtgcgc tcattttgta ttttttgctg gtattttcga
    38821 taccttcgcc attcttcagt acttaattga ccaatttgtg ccaataaatc atccggcata
    38881 ctttttatat cttgttggca gatcatttga atcatcatgt ctacttgata gatttcattg
    38941 ggcagtaaaa aatgtttttt tgctaaaagt tctagcatcg ccaatccgca tgtctctggt
    39001 acgaccctgt tcttatataa attacgcagt ttttgccaaa attttatgct tttgaaaaaa
    39061 gataattcta actgaatttc ttttgttaag taagacaaaa atcctcgcca gtatgttctt
    39121 gcaggtttaa cagaatgaaa tgtataccag ttcccatagt aatagcctaa tcgttttttc
    39181 atttccagat agcctaattg taaatttaat tggttccttt ggctatcaaa aagcagaaag
    39241 ctgcctagtg tccacaatgt ttgacatttc caatggataa aaatgtctgg catacgaatt
    39301 tttttcgctg ggcctggccc ttgcacatct acgacaaatg cctctgttgc tcctttgtta
    39361 atcgcaatat caataggtat tttgttgcga tacccaccgt ctgcatattt attttttccg
    39421 atctttcgat aagccatagc gggataaaat gaagcagagg cgagtatcca atctggtatt
    39481 tcttctgttt ttgtctgttg gatatcagtc actacttccg ctagttctgg cagtttagta
    39541 gacaccgtat ataaacgtat tttgggatta tgtttgagta aggacaaatc caaattgtct
    39601 ttgaccatct tttccaatgg agcagaagaa accccgcctt caattagtgc tgttttcgtc
    39661 atttggcgtc tttctcgata gaagcgttca cgaaggtctt caactgcagc catttctggt
    39721 aacgcaagca cttgtcttgt cgacagcttt ttccataaat tcacagcttt tttcatatct
    39781 ccttgtaaaa tcaatgcgcc gttcaacgca cctaccgagg tacctgtgat aatctcaaag
    39841 ttgatatcat gttccttcag cgcctgccac acaccaatct gataagcacc gtgcgctcct
    39901 cctccaccta aaacaagtgc tgtatgatag accagctttt tatttaattg tgtggttcct
    39961 tgatagccgt ttttatgaaa aatacgcaac cattcttctg atagaggaga ttgaaataaa
    40021 aaacaacagt tttctttaat aaaaaaacga gcgcaagctt ccatataccg aaaaaatttt
    40081 ttccattcaa aagtttcacc ttcaatcaac agattattaa aaataacatc tcctctactt
    40141 ttagcaatag taaaatagac ggtatatttt ttcgatactg ctttaaaagc acattgcttt
    40201 tttgcagaac gccaaaaagc gtctctactt tcttgatgac tttgaaaaaa tggaaggggt
    40261 tgttttgctt gctggatagg cgaatacagc gacattttct gtgaaaagta aacttttttt
    40321 atctccatcg cgctctcctt tttcttcaag tataccagta cgtcataaaa aggtcatgag
    40381 tctcgtattc gactttgtag attccataag aaaacaattc ataaaacctc ctttaaataa
    40441 aaaaatacaa aaatcacaaa aattaattga aaatgagagt gttttcaatg aaaacgtgat
    40501 tttgtaatgg aattttaaaa taaaagaaac aagctttttt aatgcaaatg ttgtaaaata
    40561 aaagagatta agaggaaata agaggaggaa catgatgact gagtcagttt atgtacatat
    40621 tgataccaca agtaatgctg ttttaacaaa agggctgact gctactgact ttgcaaacag
    40681 tatcgtgcat tttcctcaaa acttgctctt gcttgatcca tctgcttctg ctggagaata
    40741 tgaatcccat acagggctga aagtaatccg tggtacagaa aatattcagc gtttcttttc
    40801 ctcttctcgt aacaggcgtg gattcgatga attgaagtgg atcgacttta ctgatttaac
    40861 gatgctgaaa gaattgacac ctttagaaat ttcagaatta ttatattttg gtcatatgaa
    40921 aacacatctt cattcgccgt ttttttataa actacagaat aattttgtat acttcgatct
    40981 taatgatcag ctgaatcgga tttattatcg ctatttagat gaattttata ggatttttgc
    41041 tagtaaatta agttcgatct tgtcagataa gatcaatgta aaaaaatctt ttttcaaaaa
    41101 agaaacacct gttgaaaaaa tcagtgtgga attattgaaa gaaatgcgta atttgatgca
    41161 ggaagggatc attttttctt ttgatcaagt tggtttgatc gatggtaaat atcgtatccc
    41221 aatctatgtg attgaagatt atatttggaa attaaaaggc acccggtatc gagaagagga
    41281 aatcatcgct actttaatct actctgtcac cgatcgacac tggcaattgg tagaagatgc
    41341 cgatctttct tatctgccat tgaagtagat ctatttctta tcaaaaaata aaaaggaaac
    41401 aagaaactag gacatgattc tgtctcaagt ttcttgcttc ttttttattt acagctcctg
    41461 tcgtttatct ggcacaaaaa tcgaaggagt cgtttgttgg atccctaata aaacgatgat
    41521 cgttacaata gctgtactca acccgctggc accattcatt acgaaagaaa agagccaagg
    41581 attcatcccc catagtgcat acgctcccca gaaaaccact cctgcaataa aatgccaaaa
    41641 atatctggaa agtgcgccaa tcaatgcacc aaacacgatg ctttttctaa tttgtttcgt
    41701 attatcgtct acatagcctt tttggacatt tgaagccatg agccctgctg cgcctgcaaa
    41761 agtataggca atcgggtact caatcaacac ttgaacgaca cttaaataat aaacttgtcc
    41821 caacggaaaa tgtaggatgc cccaaatgaa acctgccagc atccctgcct ttgtccctct
    41881 tcttaacgca aaaagagtca atggaatctg ccctaatgaa atagaaaaac tgctgccgat
    41941 ctggatcgga atcaacgaca acaccatcgc taacgctgca acaatcgttc cttctaccca
    42001 aactctattt tttcccataa aaaaacttcc tcctttgaat aaaaaagtca caaaggagga
    42061 agcagctttc gaaaatagtc aaacagctgt ttattcacaa ttcctacgct cgcataaccg
    42121 atacaggtac gaagggttta gaaatagatt caatctcagc ccaattggac tcccctttgt
    42181 gagtttattt attatttatt ttttgtttca aacaatggac taactggttt gttttcatgg
    42241 atacgtttga ttgcatctcc cattagtccg ccaacactta cttcatcgat tttgtcgatt
    42301 ttacgatctt ctggaagata gatcgagtct gtaacaacta gacgctcgat agctgaatct
    42361 tctattcttt gtaaagcagg gccggataat actggatgtg tacatgaagc atacacacta
    42421 gttgcaccag cttctttcaa agcattggca gccaatgtga tcgtacctgc tgtatcgatc
    42481 atatcatcga tcagtacaca agttttgcct ttgacttgtc caatgatatt cattacttct
    42541 gcaacatttg cttttggacg gcgtttgtct atgatcgcaa tcggagattt caaaaattcc
    42601 gctaatttac gtgcccgtgt gacaccacca tggtctggtg aaacaacgac aacatcatcg
    42661 cctttgattc ctttttctaa gaaataattg gcaataagtg gcgcacccat caaatgatcg
    42721 actggaatat caaagaaacc ttggatttgg actgcatgaa ggtccaaagt cagcattctt
    42781 gttgcaccag ctttttgaat catgtttgct actaatttcg ctgtgattgg ttcccgtgca
    42841 cgtgctttac ggtcttgacg cgcatagcca taataaggca tgactacgtt gattgttttt
    42901 gcacttgctc gtttcagtgc gtcaatcatg atcagcaact ccatcaagtt atcattgact
    42961 gggctgctcg ttgattgaat aacatagaca tgcgatccac gaatactttc ttcaatgttt
    43021 acttgaattt ctccatcact aaattgagta acagatgatt ttcctaactc gacgccgact
    43081 gctgctgcaa ttttctctgc taatgggcga ttggaattta acgcaaaaat ttttaatctt
    43141 gggtcaaagt aatgttttga catggggacc tccactttct ttttcacaac tctagaattc
    43201 tacctaataa atagtagtca tattttttaa ataaatcaag tgattttgca aaagtcttag
    43261 tttagataag ggagtttttt tgcatatcct tctttattga cttgtcttgc tctcgcaatt
    43321 gccagcgcat attctggaat attgtctgta attgttgacc cggcagcaat cgaactgttt
    43381 ttagctacct cgacaggtcc gatgatattc gtgctagaac caataaagct gtggtcgccg
    43441 acagtcgtat gatgtttgtt ctttccatca tagttgacga agacaacacc acatccgaca
    43501 ttgatttctt ctcccagcgt agcatcacca acataagtca agtgaccgac tttagttcct
    43561 ttgccgattt gagcattttt cacttccaca aagtttccga tatggacgtt ttcgccaatt
    43621 tcagcttttg gtcgtaaatg tgcataaggt cccacatctg catgtttctt gacatggctg
    43681 ctttcaatga ctgagttttc aatgacgacg tgatcctcaa tcctactgtc tacgattttt
    43741 gaatgtgacc cgatgacaca atcagaacca atgactgtat ttccttggat ttgtacacct
    43801 gcttcgatca atgtgtctgg accgatttct acgcctgcat cgatgtatgt cgttgctgaa
    43861 tcgacaaagc ttacgccatt gaccatatgc atctggttaa ttcgctttct catgatctca
    43921 tttgctttag ctaacgcgat ccgatcattg actcccattg attcttcaaa atcatccgtt
    43981 tgataagcag caactgtatg gccttcttct tttaagattt cgatgatatc tgttagatag
    44041 tattcacctt gagcattgtt tgttccaact ttgcttaacg catcgaacaa agcttcgttg
    44101 tcaaaacagt aagttcctgt attgatttct tgtacaagag cttcttctgg tgttgcatct
    44161 ttttgttcca cgattttttc tacgatccca atatggtcac ggatgattcg tccatatcct
    44221 gttgggtttt ctgcttgtgc cgtcaagatc gttgcacttg cattttttcc ttggtgatat
    44281 tcaaaaagat tgttcagcgt ttcagtagtc aataatggtg tatcgccact gataactaac
    44341 gttgttcctt tttttccttc caagaaagaa gcagcttgca aaacagcatg tcctgttccc
    44401 aattgttctg cttgtaacgc atatttactg cgttctccta gttgtgcttt aacttgttct
    44461 gctccgtgtc caacgatcgt aatcacttca tctggtttcg tttcactgac ccgattaatg
    44521 atatgttcta ccataggttt accagaaacg ggatgtaaaa ctttatataa ttttgacttc
    44581 atgcgggtac ctttccctgc cgccaaaata attgcataac gtgcgtccaa ctgcgtcact
    44641 cctaaatata gtttgctgat ttcagtttat cccagtatac ccattttttc aacaaaacaa
    44701 gtgattttct tttatcccag cgttttctgg ataaaaaaat agatagacca taacgggcct
    44761 atctatttgg taaccggtgc tctcctgcca actcctcggt cacaagtcac actactcata
    44821 aaacatgaaa agctgttttt tgaaatcgtt tcttgtgact tagagttaaa cggtaggctt
    44881 acaacctctt cattaaacgt ttttcaatca gctgaccttc ggtattagct caataaacgg
    44941 caaaatatga ggagcaattt ttccgtttat tgagctaata ctcaggacag gacgctgatt
    45001 tcacaacctc tagataaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat
    45061 gaaaaaatgt ggaaagccat tttcacaaaa tttttcttac tactcgaaac taagcggctt
    45121 tttcacaacc tcttattctg aaaaataatt tcctggcaca accgtaatat ttttcgtttg
    45181 agtatctaca tctttgacat aaagcagtga cgtataatca tcgatggcgc gatgaccgtt
    45241 gaattttgat tcggcaaata cagtgatgcc aacaagttct gattcgaatt cttcaatcaa
    45301 gctcttcatt ccgttgactg ttccgccgcc tttcatgaaa tcatccacta caaggacacg
    45361 tgaaccacgt ttcaaacttc tcttagacag ttccatcttt tcaattcttt cagaagatcc
    45421 agacacatag ttgacactta ctgttgatcc ttctgttatt tttgagtccc ggcgtacaat
    45481 aacgaatggc gcattcagat aataggacac agcttgggca ataggtacgc cttttgtggc
    45541 tacagtcatt acagcatcga ttttttcacc aaggtattta gaagcaatga ttcttccaac
    45601 ttgacgcagc agttcaggtt ctcctaacaa atcagagaga taaacatatc ctcctggaag
    45661 cagacggtct tgttctgata gacgttctgc tagagattca atatattttt tcgcctcttc
    45721 atatgaaatt tcagggatga acaatacacc acctgcagca cccgggattg tttccaaggt
    45781 tccataccct cgttctttaa atgttttctt gatgattccc aaatcttcac tgatagaaga
    45841 tttagcagat tcataacgtt cggcaaagct tgttaaggaa atcaattcat gcgggtgatt
    45901 caataaatac tgtgtcatgt caactagacg ttcactgcga cgtactttca cctattccaa
    45961 ctccttttaa gaaattttat cgttttacat tatataagtt tttcttcaaa aattcgagca
    46021 ttttatgaaa aaataagaaa atagttcggt atttattaaa ataaaaagaa gcctataaaa
    46081 aatttttcgt ttttataagg cttcttttct ttataattta gttatttttt cttctgaagg
    46141 caaccaatat tcgtttgatg atatttacaa tcaggaacaa agcaataaag actaatgtga
    46201 ttgttgctcc tggaggtgta tctagataaa acgagctagt tagtcctgac agcatcccta
    46261 gaaatcctac acaaacactg atagcaatca cgacattgaa gcttttgcct aatcgcatac
    46321 cgattgctgc gggaagaacc atgattgcgg atatcaaaag tgcaccggca atcgggatca
    46381 tcacagcaat agccacacca gtaatcacat tgaacatcat cgacatcaaa tgaacgggta
    46441 gcccatccac gtgggcggta tcttcatcaa aagtcagaac atacatcggc cgtttgaata
    46501 aaaagaacaa tataccgatg gcaacgaata agattcctaa aaagatgact tgctcccatg
    46561 taatggtcac gatcgaacca aacagatagg attggatact tgttgctgaa ttccctccgc
    46621 tcaaattcat caatactaac gctagcgcca aaccaccagc cattaaaatg gcaatggaga
    46681 tttctgaata ggttcggtag atcgttcgca gatactccaa tatcacagct gctaatacaa
    46741 cgactagcat ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac gctacccctg
    46801 ctaaagagac atgagaaagc gtatcagcca tcagcgattg tcttctgatc actaaaaaca
    46861 cacctagcat cggggcgatt ccggcaatga agatagcggc aagaaacgct cttctcatga
    46921 attcatatga aagcaacgcc attgagaatc ctcctttctc accagtcgga tctgtctgtc
    46981 cgcatactgt ttgatatctt catgatcatg ggtgatcatt agaactgatt tcccatggac
    47041 atgcgcacta tgtcggagaa gacgataaaa ttcatttctg gattcttcat ccatacctgt
    47101 tgttggttca tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg ctaaactgat
    47161 tcgctgcttt tgtccgccag acaattcacc tattcgccga tttcgcatat cccacatccc
    47221 tacagcttct aaagcacgct gtacatgttg atgatctttt tcagagagac gcttgaacca
    47281 gcggcctctt ggatatcttc ctgaacggac taattcgatc accgtactag gaaaacctgc
    47341 attgaaagat gcaacttgtt gaggaatata accgatactt aatttttttc cttcttgatt
    47401 tttctcagca atctttatca ttccttttga aggtttcagt aatcctaacg atgctttcac
    47461 caaggttgac ttggctgctc cattttctcc agttaaaatg acaaattcac ccgcatctac
    47521 atgataagag acatcttcta gtacaggttc atcatcatag taaaaggtca aatcagatat
    47581 ttcgatataa cgcaccttac aacctccatt ctaaaactta attgatactt tcttttaaag
    47641 ctttcagatt tttttccatg acagaaatat aattttcgcc tgctttgatt tgttcattag
    47701 tcaagctttc taaaggattc aacacttcta gcttcactcc tgtttcattc gacaatgtct
    47761 cagctacctt cgaagaggca ttttcttcaa aataaatcac tttgatgttg tttttcttca
    47821 caaattcttt tagctcagct aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg
    47881 aaatcgctac ttgttttaag tcgtattgat tcgctaaata agcaaaagca gcatgctgag
    47941 tgacaaatgt gcggtttgtt gcatctttta gctcatcgga gtatttttga tccagtgctt
    48001 caagcttttt gatataagca tcggcattct tcgtaaaagt ttccttttta tctggatatt
    48061 tttcgcctaa ttcttcagca atcgttctga cttcttttat agctaatacc ggatcgagcc
    48121 aaacatgtgg atcgtattca tgtgaatgac catgcccttc ttctgattct tcatgttcgt
    48181 ctgcttcttg cgattctaaa cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt
    48241 tgctatccac tgaatccttt aacgaatcca caaaaaattc catgtctgaa ctattataca
    48301 cgaaaacatc tgcatcggaa atttctgctc tttcttttgc acttggttcg aaatcatgcg
    48361 gctccgttcc agcagggatc aaaagtttca cgtttccttc atcccctacg atctcttttg
    48421 taaaatcata catcggataa aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt
    48481 ccttgttcgt atttccacat gctgcaaaca gtaaagcacc tatcattacc gtagctgtca
    48541 aaagatattt tttcatttat atgctccttt caatgaacaa atcggaattt tacgatttat
    48601 taacgtgaac taatgtatca aaataaaaaa acacagtcaa tagaaatcca tcaatttctg
    48661 atttaagcat gtatattctt gattaaatcg taaaatggat gagttgctgc tcatatctag
    48721 ccatttcact acaataaaaa actgaagaat accattttag gattcctcag tttttcatca
    48781 cctattttac ttcttatatt ttattgcagg gttcgtacta aataaacttc ctcacaaaaa
    48841 ccttttaacc cattatatac tctctgcgct cttgaataat gttgacacaa ggcaaaaacg
    48901 gttggtccgc ttccactcat caatgcagca tctgctccgt acttgatcat acgctctttg
    48961 atttgatgga tgactggatg ccgctttccc gttatatctt ctaaactgtt tcccattttt
    49021 ttgatcatct gctgataatc ttgctctcgg atggcttcag caagtccgtg gatgtcttga
    49081 tgttggatgg cttgcgtatc tacttctcta aacactgtac gtgtagagac actcattctt
    49141 ggtttgacca acacaaccca gcactgtggc atagaaggta atacttggat ttcttcccct
    49201 ttgccaccga caaatgccgt acttccgtag acacagtaag gaacatctgt accgacttcc
    49261 aaaccgattt ctgccagttc ttctaacgaa cagcccagat tccataattg gttgatccct
    49321 cttagagcag ctgcacagtc tgtacttcct ccgcctaatc cagctgctac tggaatattc
    49381 ttcttaacaa agatcttgac accggttttg atattgtatt tattttttag caataaagca
    49441 gcttgataaa cattgttccg ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg
    49501 attttgtctt ctgttatttc ttcaaaaaat agatgatctg ccaagtctac gctcgacatc
    49561 accatttcca actcatgata cccatcttgc cgtctatata atacatccag tcctaaattt
    49621 atcttggctg gcgctttttc aatcaattcc atcctctcac ccattcgaaa agtctaagtc
    49681 gcaagtattc tatcataggg aaaaataaaa gtatagctta tgtctgccta gtggttgatt
    49741 ttgtcatcgt ttcaacagct ttcacagaat tttctcttca taatccaaac aagttctagt
    49801 gattcttata attctgtgag cacttcttct gccaaactcg gaacatttcc tttttctttt
    49861 cctatcagac tgagtagctg catggtagca aatccgaaat tacttccttt cctataagca
    49921 aaataacgtc cttcccaatg atcgacatat tttaatttct tttcataagc agactggaag
    49981 gaatagaaaa aattaccata attatatacg atatgcatga ctttttcttg aaagaaagaa
    50041 aaatctgttt ctccaacatt agctaatgga gctaagccta gattggcttc cgagtagccc
    50101 attttctcgt atacaataaa cagattggct aacagatagt cagctaattc ctcagattct
    50161 ttaggtaaga cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg
    50221 atgaagccaa cgactttttg atctttacgg acaataccga caccgctaga taatacatat
    50281 gatttatcaa atcgtcccac agagaagtat ttttctcgtg caccttgaag ccatttttgt
    50341 gaaacttcat ttacagcttg atgtaattct tccggcagtt gatcgtacca agtaaaatgg
    50401 tatccttctt tcatcaaacg ttgatattca agttgctgac tcagccgaga caagtcttct
    50461 gttgctagat caacaatccc tgcttcacct actttcgtga agtgaaaacc caattcatga
    50521 agcatcacta cgtagctttc tgatatctta taaaacgcaa gctgatagcc taaacaatct
    50581 gcttgtttca taaactgatt cgttgctgcc cgcatcattt taggattgcc aactggatct
    50641 cctaaaacaa agcatttgtt tcctttgatc tgataaccaa aaacgacttg atcttggtcg
    50701 tcttcttggt aataaaaata actgtaacct gacaagcgaa gctgatggct tgtagctgtg
    50761 cctccgtatg tttccaataa acggtcaaaa cggtcttttt cgtactcact gccaagcttc
    50821 gtagtatctt cagctaaata ttgataaagc gccactaaag ctagcaatga gatgcccaaa
    50881 ccaataagcc ctgagaacca cacatcatct gaaggaaaca aaataaaatg atttcctgtt
    50941 attcgaccga ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa
    51001 aagccgaaca agctagcatc aaagattata gctccccaag aataaacgaa ttttgttcga
    51061 taaaattctt ttctggataa ccacacagct aacagaatca agacatatac caatatcaac
    51121 tgccaagata acgtacgtgt gattgtattt aaaataccaa aagatagtaa agcaatcgta
    51181 ggcagaaatg cctttttgac ttttactgaa agtgctctag caaggccgag taataaaaag
    51241 ccaaccaata gattcactgt ctgatctaaa aaattgaatg agaacggcaa taatacttga
    51301 aatagccgac tgattgcaga cagattagtg attgttgata gaagtaccat catgatcccc
    51361 gcaaaataga cagcacttac gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga
    51421 atgttatcta aaaatcgatt gattttgact cctgtatgtg tgatgaacaa tagaagtccc
    51481 gtcataaaag gtagaacata ataaaacaac cgataatata atagccaaac tactgctaca
    51541 tcttgccgaa cagacaattg actcattcct aagatcatca aaacgtcaaa agtccccatt
    51601 ccacctggga ccatcgtcag cattcctatc aatgtagcaa tcatgaacat aggatagatc
    51661 gaaagcatcg aaatatctat tctcatcaac ttgccgatga tcaaaaatac cgcaagggct
    51721 cccgtccatt gtccaaagga tgccagtagc agaccaagca agccttttgg ataaaactcg
    51781 aagaacaatg tctttctctt tagataagca aaaaagaaaa tgactggtgc taaaaaacta
    51841 ccgccgaaaa gccagatcca atagcttcga aaaatacttt ctgactgaat aaaaaagaca
    51901 tcaataaatg taagaaacga ccatacagac aaaccagtca gcataaatag tgcgactttt
    51961 gatacagtgg ctaaaaccat ttttctattt gtctgctttc cataaaaact agcccgcaga
    52021 cttgctccta ccacaccacc aaaaccggcg agattgttga tcgtgtttgt tacccacgca
    52081 gcaagaagaa actcttttct ttccattttt ggttttcctt gtttttctaa gactctgatc
    52141 gtcacccaat catacaacaa catcggagtg acacccagca gtccagccag aatcatgagc
    52201 aggatcgtca tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca actcatgcca
    52261 ttagcaatat ttgcgacttg atttgccaca aagactaaca cggagccgaa aaaaatcagt
    52321 tttaaaatca gactatgttc ctttatccat tgcagcatca agtatcgttt atttttcaca
    52381 ttttcccatt cctaacttgt atacttttgt gtcatacccc aatgttatct tatttatttt
    52441 aatttttcca ctttagacct gttctttgct tgcgatgata gaaaaagaaa gatatcctaa
    52501 tgctaaagga gaggataaca tgaaagtagg atttatcggc acagggaata tggcacaagc
    52561 tattattgat ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag
    52621 tggacatttt gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag
    52681 taatcaggaa gttgcatcac aatcagatat cgttctttta gccgtgaaac caaaaatcat
    52741 tgaaaaagta ttggaagaac taagagaata tagccagaaa gtcttattca tttctattgc
    52801 ttcaggaaaa tcattaatag atttagaaac agcgcttgaa gatcctcagg cctccatcgt
    52861 acgtgtgatg ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa
    52921 tgaagctgcc acggaggaac agcttcagca agctacgatg atttttgaag caatcggcag
    52981 cgtctatcct ttagcagaat cagatttcag tacattcatt ggccttgctg gcagctcacc
    53041 cgcattcgtt tacatgctga tcgatgcgat gggacggact ggtgtactga atggtttgtc
    53101 caaacagaca gccactgaga ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct
    53161 ttcaagtgat aaaaatccat gggaacttgt agaccaagtt tcttctcctg gcggtacaac
    53221 tgttgcaggt gtagttgcct tagaagaagc aggattcatc ccagcaatca tcaaaggaat
    53281 caatacgacg atcgagaaag atcttgagat gttgaataaa aaataatgat agcaatgttt
    53341 tttcgtgcct tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc ttttgtattt
    53401 tttattattt agcataatca gttgcacgag tttcccgaat aacgattact ttgatgtgtc
    53461 caggataatc cagttcttct tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt
    53521 ctaaatccga gatttcttct ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg
    53581 cgtaacttga ttctacaccg gcgaagctgt tcgaaatatt ttccagactt cttaaacgac
    53641 ggatgtagtt ttctaatgat tcacttcgcg caccaggtct tgctgctgag agcgcatctg
    53701 ctgcagctac aaggacagag ataacagaag tagcttccac atctccatga tgagaagcga
    53761 tcgcgttgat tactactggg ttttctttgt acttggcagc taattccgca ccgatctcaa
    53821 cgtgagatcc ttcaatctca tggtcaagtg ctttcccaat atcatggagc aacccggcac
    53881 gttttgctaa ttggatatct tcgccaagct ctgctgctaa gataccagct aattttgcaa
    53941 cctcgaccga atgcttcaag acgttttgtc cataactagt tctgaatcgc agacgtccga
    54001 tgatcttgat tagatctgga tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc
    54061 cgtattcgcg aatacgttca tccatttctt tacgagattt ctcgaccatt tcttcgatac
    54121 gcgctggatg gattcgtcca tcttgtatca gtttttccaa ggtcatccgt gcgatttctc
    54181 ttcggattgg atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat
    54241 cgataccagt cagcgtctca agcgttcgaa tattacgacc ttctcgtcca atgatacgac
    54301 ctttcatttc atcattcgga agggtaacga ctgaaacagt cgtttctgaa acttgatctg
    54361 ctgcgcagcg ttgaatcgct aatgataata agtttttcgc tttgcgatct gcttcttctt
    54421 tggcacgctg ttcagattct tttaccatta gagttaactc atgattcagc tcttcttcag
    54481 tcgatttcat gatgacatct cgtgcgtcct cttttgataa accagcaatt ttttctagtt
    54541 ctttttgttg atcttcaatc attttttcaa cgtctttttc tcgctcttca attaattgct
    54601 gttttgaact aagtgcttct tctttacttt caagtgagtt ctcgcgtttt tctaacgagt
    54661 catctttacg attcagcgtt tgttcgcgtt gaatcaaacg attctcttga gattttaatt
    54721 ctaacttact ttctctcagc tcactttcaa cttcagaacg gtatttctga ttttcttcct
    54781 tagcttctaa caatgcttct ttttttagag tttctgcttc tttttttgca ctatcgatga
    54841 ttccagcagc tgatgattgt gcaccagcta tttctttctc gtgacgtgac tttgcgacca
    54901 taaaccctaa accaagaccg acaattaaac cgatgatagc gaggagaatg ttgaatacca
    54961 taaaatccac ctccatacta tctttttcat ttttatcatc tatacagctt ctatgataaa
    55021 ttattctaaa ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac atgcacaact
    55081 ttattctaat gtttgtaccc cgtagtgtca actttaaata aacaaagaaa cggctttcta
    55141 cttattcatt aaacgatggt ttttaaaata aatgacacta gtttcatata ggtaaacctt
    55201 taaattgaga aacttttcta ttaaattatt ctttgtagat gaacagatat ttttatttta
    55261 tgtacccact cgtataatta aataactcac gatttcttta ttcaataaaa aaacggcagg
    55321 tcaaggagag tacctactct tcagaccagc cgtttttgtt tatcataatt tttattcttc
    55381 tactaaaggc aattcttctt gtcctttttc atcttctggg acagcgactt cttcaccaat
    55441 accataagct gctctaacta gagcagaaac ttctgccatc atttcaggat gattagccat
    55501 atagatttta gcgttttccc gtccttgacc aatccgatct tctttataag aataccaagc
    55561 accgctttta tcaacaatgt ctttttctac tgccatgtct agaagttctc cttcttgtga
    55621 gatccctaaa ccatacatga catctacttc agcgattttg aaaggaggtg ctactttgtt
    55681 ttttacgact ttgatctttg tacggttacc aacaatatct gtaccttgtt tcaattgttc
    55741 tgcacgacgt acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt
    55801 gatttctgga ttcccaaaca tcacgcctac tttttcacgg atttggttga tgaaaatagc
    55861 gattgttttt gttttgttga tcgaaccaga gagtttacgc aatgcttgag acatcaaacg
    55921 tgcttgtaac ccgacatgtg agtcacccat ttcgccgtcg atttctgctc gtggaactaa
    55981 agcagcaact gaatcgacta ctacgatatc tacagcccca cttgatacta aagcatcagc
    56041 gatctctaga ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac
    56101 acctaatttt tgtgcatatt gcggatctaa cgcatgctca gcatcaatga aagcggccgt
    56161 tccgccattt ttttgtactt ctgcaatagc gtgtagtgca actgttgttt tacctgaact
    56221 ttcaggtcca tatacttcga tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac
    56281 atctaacgca agagagccac ttggaattgt agagatttgt tggtcgattt tttcgcccaa
    56341 tttcataata gagcctttac catagctctt ttcaattttt ttcagtgcag catctaaggc
    56401 tgctttacga tcatctgcca atacgataat cctccttata tttacaaatc agagcaacat
    56461 ttcattatcc ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat
    56521 tcgcatttat cattttctta aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc
    56581 attatatctg atagcagcac gatctctggc aaagtgatat ttttccgaaa ccacgccgtc
    56641 tttagatgaa atagcgatcc atactgttcc tgcagattga ccttctaacg tgtccggtcc
    56701 agccactccg gtgaaagcta cactgtaatc tgttcttgct atctcacgtg cttgaatggc
    56761 catctgctcc acacattctt tgctgactgt tccatattct gctaatagct ccggatcaat
    56821 ctccagaaaa ccagcttttg tttggagcga gtaggtaacg aatccgcctg gaaatacttc
    56881 agatacacca gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca aactttccgc
    56941 tgctgttaca gtctgcttgt tttctttcag tagttccact acgacttttg ctagcgaatt
    57001 atcatcgccg tatccataaa aatattcccc tactcgttct tggatttttt cttctaatgc
    57061 taaaagagct tgattccctg cctgaacatc atttgttttc actgtcagtc gtaatgtcac
    57121 ttcatttggt ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt
    57181 tagctcagtg accagtcgcg attcaccaat cccataaaag cgcaaaactt tagaaattag
    57241 tttttcttca gaaggaaaac gttcctctag taaaggacgt acttgttcca caaacattgg
    57301 tttcaactca ttaggcggac cgggaagaag gatatacgca tgtgtatctg tttgataaaa
    57361 agtccctaaa gcaagccctg tacgatttgg tagaggaacc ccgccttcga taatctggga
    57421 ctgttgcaga ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg ccaataattt
    57481 tttgtatcct tccgtatttt gtatgagaga cttaccgagg tgagccgcag ttgcttcttt
    57541 tgtcaggtca tcttctgtcg gtcctaatcc gccacataag agtatgagtt cacttcttgt
    57601 ttcagctaga gcaatcaact cttctaaacg ctgctgatta tctcctacaa ctgtttgata
    57661 ataaacttcg atacctagat cagctagctg ttctgacaag aaagtggcat tcgtattcac
    57721 gacttgtcct agtaaaagtt ctgttccgac agctattatt tctgctttca ttacttcccg
    57781 ctcccttata tagtatacgc tgtattttta tattttttta tttaaaaaga gggcaaggag
    57841 ctgtgacaaa aatcttgtca cagctccttg ttccaaataa acagtgttct cctgccaaaa
    57901 cctcggtcat aagtcacaat atacataaaa catgaaaagc tgttttttga aattatccct
    57961 tgtgactcag agttagacgg caggctcaca acctctccac taaacgatac ttacattgat
    58021 cctttgaaca caccgctgtt tttgatgaag taatccacac ctgaatagat agtaaagatc
    58081 agacaagcat aaagcatgat ttgatctact ggaataccga tcaggctgaa tgggatatta
    58141 ttgataaaca gcaagataat agcaaccatt tgtgtagccg tcttgacttt tcccggccaa
    58201 gctgcagcca tgacttctcc atgttcgaca agtagtaaac gcaagcctgt gacggctaat
    58261 tcacgacaga caatgatcgc tactacccaa ccaggggcct ttccttggtc aaccaacatg
    58321 ataaaagcag tcataactag cattttatct gccaacgggt ccgcaaactt gccaaaatta
    58381 gtgacaaggc ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat actagctaaa
    58441 gcaaaaataa ctgcacctac tagctgtgtg acagccagtg tggtgtctcc tacgttaaat
    58501 tgtccccaat ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat acaaattcta
    58561 agtacagtta atttatttgg taaattcaca ctatcgactc ctttcttccc tattctacgt
    58621 gactatcagc gaagtttcca ccgatattat ttacttttcc aaatgaattt attcaaagga
    58681 gtatttttat tgaaattgat ctttttgaat taacgaagaa ggaccgcgac acagatttgt
    58741 cacgatcctt cttttttata cctacaagta aacagatgcg taaactaatt tatacgatca
    58801 ttctgcgtat tgaagagtaa ggttcacatt ttttggactc tgattgtttt cccctggatt
    58861 aactggtacg gattctccat tcgctttgat cgttgcattg cttgctgcac caatcgtgat
    58921 ggtcgcattt gttgctgttt ccggtaacac ggtactctgt gtttcatttg ctgccagtgt
    58981 cccttgataa acataggcat tgtctactag cacacctaac catacacgat tgacagcagt
    59041 aaattcaagg gttacaggct tggtagcgtc tgtgatattt actgcgatgg cactgccaga
    59101 atcttctcct gtagctattg ccattttctt ttcttcactt gattttgttg ttgattctac
    59161 tgtagaagac tctttggtct gcgtgctttg ctctgaggaa gaggactcct cgactgatcc
    59221 ttctacttta agcgaagaag ctgtgtctcc gattattgga tcagcttgac gatcttgcca
    59281 agtcatatag cccacgacaa cgacaatcgc caaagcaacc aatcctaata aaatgacagg
    59341 aagacttgtc caaaagcgac ttggatgcga ttcttcctga tgcattgctt ttctagaagt
    59401 acttactgtt tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc ttttcccatc
    59461 aaaaacatcg actaaatggt ctccatcttc tcctacagct tcagcgtatt gccgaataaa
    59521 tgcccgcacg taaaatttac caggcaattg gtcaaaatca tcattgtcga ttgcttccaa
    59581 ataacgtttt tgaatttttg taatctgctg taattcatct aaagatattt ttttattcag
    59641 gcgtgcttgc cgtaattttt ctccgatggt ttcaaaagcc actcgttcat cttctccagt
    59701 ctgtttgatt ctccgaagca tctgctgttg caacgaaaga cgttatgttt gggtgctgtg
    59761 acaaaagtca tgtcacagct ccttttttcc gaataaacat tgttctcctg ccaactcctc
    59821 ggtcacaagt cacaatatcc aataaatata agaagctgtt ttttgaactt gttccttgtg
    59881 actcggagtt aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc
    59941 ctcggtatta gctcaaaaac cggcaaaata taaggagata tttttccgta tgtttctcta
    60001 atactcaggt caaaacgctg atttcacaac ctcttcctta aacggtgttc aaaagcttgc
    60061 atctgcggta ataagtccaa ccaagttaaa aacattaaga agctgttttt acttggctgt
    60121 tcttattact tgatgcaggg cagctttttc acaacctctt ggtacacggt gttccagaag
    60181 tagcttcttc ggcaataagc cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt
    60241 ccttcttatt tcttgaagct gaccacttct gtcacaacct ctttgattac ttcgtttgat
    60301 tttattttaa tataagttga ttaaaagaga gcatgatttg ttttattttc tcactaattt
    60361 ttccaacaca actaagaata ccataaaagc tgattttgga ggaggttcct actacaagtc
    60421 taaaacgaaa tttaagactt ttcctcataa aatactggtt tattttggtc tcatgtaaaa
    60481 ctcgctgaag gcctcttcac ttataaatgc ttccccagct gccagtacgt ctttcatttg
    60541 gatcgaatcg atgatttcag gaagatcaaa taaagttcga tcgccaaata aactttgcgt
    60601 aaattgattg gcaatatatt cgatcgaatt taatgattgg aaatattgtc caagcatttt
    60661 cttcttcagc aaatcgagat ttgtttctga cacttccgga tcatcagcga atccaagaat
    60721 gatttctttg actttttctg ctgctttctc tggttcgtcc gtatctccgc tgaagtccgc
    60781 aaagtgaaat tcacgatcca agctgaattc aaaaccaaag ctgtcatcga taattccttg
    60841 attgtacatc gctagatagt ttcgagaagt attgcctaaa atcatttgga acaacagatt
    60901 gattgctgtt ttatagcgta acagttctgt tccttcttgc ggcaatgtgt ctaatccttt
    60961 aataccaagt acaaatttat ctcgtgtaat ggctgcttcc aatgcacttt gtttgatgat
    61021 ttcttttgtg ttttcaggga aataacgtac aatttcttgc tttggaggga aattttttgc
    61081 ttcttggttt tcccgaataa gtttcatcaa tttctcaggc tccatcttac caactacaaa
    61141 taaaaccata ttgcttggct gataaaacgt ccgatagcac gtatacaagt cttgagctgt
    61201 gattttgtcg atactttcaa ccgtgcccgc aatatcgata tgcaatgggt gtgtaggata
    61261 caagttattt agtatgccaa aaaacatccg ccagttaggg tcgtcttcat acatttggat
    61321 ttcctgaccg atgattcctt tttccttgtt tacagtttct tctgtgaagt aaggtgcttg
    61381 tacaaaatcg atcagagtcg tcagattctt ttctacctga tccgtcgtag aaaacagata
    61441 gctggttttc gtaaaacttg tgaaagcatt ggcggaagct ccttgtttcc caaataattg
    61501 aaagacatca ccatcttctt tttcaaacaa cttatgttct aaaaaatggg caattccatc
    61561 tggaactttc accttttctt tttcaccata gggaataaat tcgttatcga tcgaaccata
    61621 attcgtactg aacaaaccat atgtcttgtg atagtcattt ttaggcaaca aatacacggt
    61681 caaaccgttt ggcagtactt catgataaag tgtttcattg atttgctcat attctgtttt
    61741 attcatttgc atttcctcct gctaaaaaga agattgcctg caattcgatt tgctcagcta
    61801 cttgttgtat ctctttgatc gttacgctct ccatacgttt caaccattct tcatcagata
    61861 attttgtttc cggcagccag ctgtccaaaa aagaagcttc gattgccgct tgcggagagt
    61921 ctagagaaag cagatattgg tttctcagca ttgcttttgt ctgagacagt tccaactctg
    61981 ttatttcacc atttcgcaaa ctttctaatt gctcgtgaat cagatgaagt acttgatttc
    62041 ggttcttttc atcgataccg gtttggacac tcatgaatcc tcggaacgta tccacgctac
    62101 tagaggcata ataagctaga ctttctttct ctctgacatt catgaaaagt ttagagtgag
    62161 gaaaaccgcc aaataaaccg ttaaagacca ttagcgcaaa acgttctggt tcatcgtaat
    62221 acacatttgt ctgataagct aggttcagct ttgcttgaac gattggctca cgtacttgtc
    62281 gttccttcac gatattcact tgaggttggg tataaaacag atctggataa actgtttccc
    62341 gcgactcaaa tggtaattgc ccaactgctt cagctgcttt gtccggatca acatcgccga
    62401 caacaaaaat gtcgatttga tcatttgcca tcatttcttg atagtaggac gctagcgaac
    62461 tagcagtgat cttgtccaaa tcttcgacta cgcccaaccc tgggatcttt tgttccgggc
    62521 tgttttcaaa ataaagttct tgcaaggcat aggaagcaaa agtttgttta tcttccttca
    62581 aactttccaa atatagacgc agattgttct tttccaagtc aaacgtcaac tgatcaaact
    62641 gatgattttt aatatttgga taaaataaaa cctcttttaa aaaatctacc gcttgagaaa
    62701 aaaggtttgg atcattgaca tattcaccat tcacaaaact gatccctgcg tttagccaat
    62761 gtaaatttcc tttttttcta actgataatc caaagcttgc gccataaagc tctgcaagtt
    62821 gcgcacttaa ctttgtctga tccgaataat tcaaactatt tgtttccaac atgctgctga
    62881 gaagtgaacg tttcgtgatg acttcttgtt gcagacgcgc agtaaaacgg ataaaaatcc
    62941 ggatcgtttt gtattgtttt gttggtaaga catgaagatt cacgccttct gccaattgat
    63001 acaccatatt tatatgctcc ttttcacagt tgattgcccg cttactcttt taatgggtca
    63061 acctgccttt tttacttatt cctctgcttg aaatttataa gttcaggcaa ttcgccgttt
    63121 tttctaaatg atgttacact ttatagtata gcatagatgt tttttatcaa aagaaacaga
    63181 cggctaaaag cgtatattgt ttttttattt tttatttttc gatatactaa agacaaataa
    63241 aagaagggag ggtcattcat gatcaaagaa ttcaaagagt tcatcatgcg tggagatgtc
    63301 ttggatcttg ctgtcggcgt tgttattggt agtgcgttta caagcatcgt aaaccaagtg
    63361 gtgcaaggct tgatcacacc actaataggt tggtttgtcg caatgatcac aggtacttct
    63421 gatttagaag gagcactttc aattcttgac tggtcgccga caaaaggcgt cacatttgcg
    63481 tttggtaacg ttattagtgc tatcattaca tttctgatca ctggttttgt cttattctta
    63541 gttgtcaaag cggcaaaccg tgcgaagaat ttacgctcca aagaagagca agtggaagtg
    63601 gaagaaactc ctactgcaga agattatctt agtgacatac gcgatcttct tgccaaacaa
    63661 gtggcagaag acaatacaca tccagtagag caaacaaatc tttccgatac ttctaaataa
    63721 taaagaatct aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt
    63781 aacttgctac ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat
    63841 ctgagattgc cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga
    63901 tcacgattgg tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct
    63961 ttcttcctaa accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac
    64021 tctccgcttc ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa
    64081 acgtatgcca agcttctcca tcaatgtcat caacttcttg ataaggacac tgttgttcca
    64141 caacaaaaac tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt
    64201 caaatttctt tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat
    64261 accgattcgc acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta
    64321 aactgcttca gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta
    64381 agatactcgt tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata
    64441 ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa
    64501 catgattggg ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg
    64561 catacagttc acctctacta tcaactatac cacatatttt taaaattttc aagtttac
//
LOCUS       DAFFSV010000003        64012 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFFSV010000003 DAFFSV010000000
VERSION     DAFFSV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.003"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(254..757)
                     /locus_tag="LR110_000116"
     CDS             complement(254..757)
                     /locus_tag="LR110_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL4821123.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1116..2141
                     /locus_tag="LR110_000117"
     CDS             1116..2141
                     /locus_tag="LR110_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL4821124.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2464..3342
                     /locus_tag="LR110_000118"
     CDS             2464..3342
                     /locus_tag="LR110_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBL4821125.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3818..3934
                     /locus_tag="LR110_000119"
     CDS             3818..3934
                     /locus_tag="LR110_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL4821126.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4862..5200
                     /locus_tag="LR110_000120"
     CDS             4862..5200
                     /locus_tag="LR110_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821127.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5294..5524
                     /locus_tag="LR110_000121"
     CDS             5294..5524
                     /locus_tag="LR110_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821128.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            5924..6295
                     /locus_tag="LR110_000122"
     CDS             5924..6295
                     /locus_tag="LR110_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBL4821129.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6569..7261)
                     /locus_tag="LR110_000123"
     CDS             complement(6569..7261)
                     /locus_tag="LR110_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL4821130.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7264..8049)
                     /locus_tag="LR110_000124"
     CDS             complement(7264..8049)
                     /locus_tag="LR110_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL4821131.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8286..9686
                     /gene="lacG"
                     /locus_tag="LR110_000125"
     CDS             8286..9686
                     /gene="lacG"
                     /locus_tag="LR110_000125"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL4821132.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9711..10028
                     /locus_tag="LR110_000126"
     CDS             9711..10028
                     /locus_tag="LR110_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL4821133.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10041..11714
                     /locus_tag="LR110_000127"
     CDS             10041..11714
                     /locus_tag="LR110_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBL4821134.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11728..13359
                     /locus_tag="LR110_000128"
     CDS             11728..13359
                     /locus_tag="LR110_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBL4821135.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(13850..15415)
                     /gene="guaA"
                     /locus_tag="LR110_000129"
     CDS             complement(13850..15415)
                     /gene="guaA"
                     /locus_tag="LR110_000129"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBL4821136.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15474..16397)
                     /locus_tag="LR110_000130"
     CDS             complement(15474..16397)
                     /locus_tag="LR110_000130"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBL4821137.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16593..17198
                     /locus_tag="LR110_000131"
     CDS             16593..17198
                     /locus_tag="LR110_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL4821138.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17506..18522
                     /locus_tag="LR110_000132"
     CDS             17506..18522
                     /locus_tag="LR110_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL4821139.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18663..19964
                     /locus_tag="LR110_000133"
     CDS             18663..19964
                     /locus_tag="LR110_000133"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBL4821140.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20010..20672
                     /gene="deoC"
                     /locus_tag="LR110_000134"
     CDS             20010..20672
                     /gene="deoC"
                     /locus_tag="LR110_000134"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBL4821141.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20693..21091
                     /locus_tag="LR110_000135"
     CDS             20693..21091
                     /locus_tag="LR110_000135"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBL4821142.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21157..22239
                     /locus_tag="LR110_000136"
     CDS             21157..22239
                     /locus_tag="LR110_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4821143.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22451..24013
                     /locus_tag="LR110_000137"
     CDS             22451..24013
                     /locus_tag="LR110_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4821144.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24006..25139
                     /locus_tag="LR110_000138"
     CDS             24006..25139
                     /locus_tag="LR110_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4821145.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25139..26095
                     /locus_tag="LR110_000139"
     CDS             25139..26095
                     /locus_tag="LR110_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4821146.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26194..27360
                     /gene="deoB"
                     /locus_tag="LR110_000140"
     CDS             26194..27360
                     /gene="deoB"
                     /locus_tag="LR110_000140"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBL4821147.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27384..28202
                     /locus_tag="LR110_000141"
     CDS             27384..28202
                     /locus_tag="LR110_000141"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL4821148.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28219..28926
                     /gene="deoD"
                     /locus_tag="LR110_000142"
     CDS             28219..28926
                     /gene="deoD"
                     /locus_tag="LR110_000142"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL4821149.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29224..29910)
                     /gene="gpmA"
                     /locus_tag="LR110_000143"
     CDS             complement(29224..29910)
                     /gene="gpmA"
                     /locus_tag="LR110_000143"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL4821150.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30082..30663)
                     /locus_tag="LR110_000144"
     CDS             complement(30082..30663)
                     /locus_tag="LR110_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4821151.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(30796..31479)
                     /gene="rpiA"
                     /locus_tag="LR110_000145"
     CDS             complement(30796..31479)
                     /gene="rpiA"
                     /locus_tag="LR110_000145"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBL4821152.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            31798..32211
                     /gene="rpsL"
                     /locus_tag="LR110_000146"
     CDS             31798..32211
                     /gene="rpsL"
                     /locus_tag="LR110_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBL4821153.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32295..32765
                     /gene="rpsG"
                     /locus_tag="LR110_000147"
     CDS             32295..32765
                     /gene="rpsG"
                     /locus_tag="LR110_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBL4821154.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            32859..34946
                     /gene="fusA"
                     /locus_tag="LR110_000148"
     CDS             32859..34946
                     /gene="fusA"
                     /locus_tag="LR110_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL4821155.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35118..36305
                     /gene="tuf"
                     /locus_tag="LR110_000149"
     CDS             35118..36305
                     /gene="tuf"
                     /locus_tag="LR110_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL4821156.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36712..37020
                     /gene="rpsJ"
                     /locus_tag="LR110_000150"
     CDS             36712..37020
                     /gene="rpsJ"
                     /locus_tag="LR110_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBL4821157.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37051..37680
                     /gene="rplC"
                     /locus_tag="LR110_000151"
     CDS             37051..37680
                     /gene="rplC"
                     /locus_tag="LR110_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBL4821158.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37708..38331
                     /gene="rplD"
                     /locus_tag="LR110_000152"
     CDS             37708..38331
                     /gene="rplD"
                     /locus_tag="LR110_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBL4821159.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38331..38621
                     /gene="rplW"
                     /locus_tag="LR110_000153"
     CDS             38331..38621
                     /gene="rplW"
                     /locus_tag="LR110_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBL4821160.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38660..39493
                     /gene="rplB"
                     /locus_tag="LR110_000154"
     CDS             38660..39493
                     /gene="rplB"
                     /locus_tag="LR110_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBL4821161.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39536..39814
                     /gene="rpsS"
                     /locus_tag="LR110_000155"
     CDS             39536..39814
                     /gene="rpsS"
                     /locus_tag="LR110_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBL4821162.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            39837..40184
                     /gene="rplV"
                     /locus_tag="LR110_000156"
     CDS             39837..40184
                     /gene="rplV"
                     /locus_tag="LR110_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBL4821163.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40198..40854
                     /gene="rpsC"
                     /locus_tag="LR110_000157"
     CDS             40198..40854
                     /gene="rpsC"
                     /locus_tag="LR110_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBL4821164.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            40857..41291
                     /gene="rplP"
                     /locus_tag="LR110_000158"
     CDS             40857..41291
                     /gene="rplP"
                     /locus_tag="LR110_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBL4821165.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41281..41469
                     /gene="rpmC"
                     /locus_tag="LR110_000159"
     CDS             41281..41469
                     /gene="rpmC"
                     /locus_tag="LR110_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBL4821166.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41494..41760
                     /gene="rpsQ"
                     /locus_tag="LR110_000160"
     CDS             41494..41760
                     /gene="rpsQ"
                     /locus_tag="LR110_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBL4821167.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            41818..42186
                     /gene="rplN"
                     /locus_tag="LR110_000161"
     CDS             41818..42186
                     /gene="rplN"
                     /locus_tag="LR110_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBL4821168.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42223..42531
                     /gene="rplX"
                     /locus_tag="LR110_000162"
     CDS             42223..42531
                     /gene="rplX"
                     /locus_tag="LR110_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBL4821169.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42559..43098
                     /gene="rplE"
                     /locus_tag="LR110_000163"
     CDS             42559..43098
                     /gene="rplE"
                     /locus_tag="LR110_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBL4821170.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43117..43302
                     /locus_tag="LR110_000164"
     CDS             43117..43302
                     /locus_tag="LR110_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBL4821171.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43338..43736
                     /gene="rpsH"
                     /locus_tag="LR110_000165"
     CDS             43338..43736
                     /gene="rpsH"
                     /locus_tag="LR110_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBL4821172.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            43768..44304
                     /gene="rplF"
                     /locus_tag="LR110_000166"
     CDS             43768..44304
                     /gene="rplF"
                     /locus_tag="LR110_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBL4821173.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44465..44821
                     /gene="rplR"
                     /locus_tag="LR110_000167"
     CDS             44465..44821
                     /gene="rplR"
                     /locus_tag="LR110_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBL4821174.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            44842..45342
                     /gene="rpsE"
                     /locus_tag="LR110_000168"
     CDS             44842..45342
                     /gene="rpsE"
                     /locus_tag="LR110_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBL4821175.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45357..45536
                     /gene="rpmD"
                     /locus_tag="LR110_000169"
     CDS             45357..45536
                     /gene="rpmD"
                     /locus_tag="LR110_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBL4821176.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45580..46020
                     /gene="rplO"
                     /locus_tag="LR110_000170"
     CDS             45580..46020
                     /gene="rplO"
                     /locus_tag="LR110_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBL4821177.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46020..47315
                     /gene="secY"
                     /locus_tag="LR110_000171"
     CDS             46020..47315
                     /gene="secY"
                     /locus_tag="LR110_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBL4821178.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47377..48024
                     /locus_tag="LR110_000172"
     CDS             47377..48024
                     /locus_tag="LR110_000172"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBL4821179.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48215..48433
                     /gene="infA"
                     /locus_tag="LR110_000173"
     CDS             48215..48433
                     /gene="infA"
                     /locus_tag="LR110_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBL4821180.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48466..48582
                     /gene="rpmJ"
                     /locus_tag="LR110_000174"
     CDS             48466..48582
                     /gene="rpmJ"
                     /locus_tag="LR110_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBL4821181.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48600..48965
                     /gene="rpsM"
                     /locus_tag="LR110_000175"
     CDS             48600..48965
                     /gene="rpsM"
                     /locus_tag="LR110_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBL4821182.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            48993..49382
                     /gene="rpsK"
                     /locus_tag="LR110_000176"
     CDS             48993..49382
                     /gene="rpsK"
                     /locus_tag="LR110_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBL4821183.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49462..50400
                     /locus_tag="LR110_000177"
     CDS             49462..50400
                     /locus_tag="LR110_000177"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBL4821184.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50428..50808
                     /gene="rplQ"
                     /locus_tag="LR110_000178"
     CDS             50428..50808
                     /gene="rplQ"
                     /locus_tag="LR110_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBL4821185.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51080..51919
                     /locus_tag="LR110_000179"
     CDS             51080..51919
                     /locus_tag="LR110_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL4821186.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            51895..52764
                     /locus_tag="LR110_000180"
     CDS             51895..52764
                     /locus_tag="LR110_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL4821187.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            52754..53551
                     /locus_tag="LR110_000181"
     CDS             52754..53551
                     /locus_tag="LR110_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL4821188.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            53754..54500
                     /gene="truA"
                     /locus_tag="LR110_000182"
     CDS             53754..54500
                     /gene="truA"
                     /locus_tag="LR110_000182"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL4821189.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(54955..57624)
                     /locus_tag="LR110_000183"
     CDS             complement(54955..57624)
                     /locus_tag="LR110_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBL4821190.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(57862..58050)
                     /locus_tag="LR110_000184"
     CDS             complement(57862..58050)
                     /locus_tag="LR110_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBL4821191.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            58320..58682
                     /locus_tag="LR110_000185"
     CDS             58320..58682
                     /locus_tag="LR110_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL4821192.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            58734..60416
                     /locus_tag="LR110_000186"
     CDS             58734..60416
                     /locus_tag="LR110_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBL4821193.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            60533..62569
                     /gene="recG"
                     /locus_tag="LR110_000187"
     CDS             60533..62569
                     /gene="recG"
                     /locus_tag="LR110_000187"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBL4821194.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            62618..63619
                     /gene="plsX"
                     /locus_tag="LR110_000188"
     CDS             62618..63619
                     /gene="plsX"
                     /locus_tag="LR110_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBL4821195.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            63741..63983
                     /gene="acpP"
                     /locus_tag="LR110_000189"
     CDS             63741..63983
                     /gene="acpP"
                     /locus_tag="LR110_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL4821196.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        20526 a        11484 c        13795 g        18207 t
ORIGIN      
        1 gacggaattc ctttctagat tgagatattg actaggaaga atgttaagat tttaagcctt
       61 taagctaaaa aaaaaccctt tttgttaaat aaggggaata tagcctgtca gtttcaaact
      121 gcaagtcgtc ctgctgcaat aatatagtag attagagatg aaaatagatt tttttaaacc
      181 aatgactgaa taaaaagacc gtcatagata cacattggaa tgtgtctatt acggtctttt
      241 tttacgaacg attttattga agacggacag ttcgggtcaa aaataaaaaa gctacgatca
      301 gaaataagaa taaaatgtat cccaaaataa tggctagacg gtctaactta tttgtcgata
      361 gatgtttgtt tagaagttga taggcccata aacctatcag tcctcctgtt gtattagtta
      421 tcaaatcagt gatgtccgct acgccaatac cgaataggaa ttgaatcaat tctgccaaga
      481 agcttacaag gaaaataatc cctaattttt tagaaaaaga aagtctcttg aaattgacat
      541 tcaaaagcac cccaaaagga aaaaagacaa tcaagttata agtggtctca cgtaataccg
      601 tcgtattttc aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg taagaataag
      661 tgagaaattg tggaagatta aatgatagct tgaataaaat caaccataat agaaagaata
      721 aataaatccc taataaaaat tttgatacat accgcataga catctcctcc atttaaaaag
      781 tataccatcg aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg accaacgtag
      841 ttatcgtacg aaaattagaa gctttttttt gttgggaatc cggtttcttt aacaaagcta
      901 agatagcgag ttgcttggaa aagttgctat cttttttctg tcaaaacgac tcggaatgtt
      961 cgttttttgt tctaatttct ccaagttgtt tactttttga tactaagttt atgaaaaatc
     1021 aagaaatagt tgttatacta ggtaatgctg acaaacggga ttggcatttc atcttggttc
     1081 agcttttttt ccaattacta ggaaggaggg atagattgtt caagacttat tttcgtttca
     1141 ataaaataca agataatcct tttcgtatca tgaatctgat gctacttatc tttattgtat
     1201 tatggttagg ttacataaat catgattttt tgatttcaag tttttcatca ttagggatct
     1261 ttactttttt ctattatcaa aacataccga tgaaatattt gctgaaacgt atgtttctga
     1321 tcggttgcgg actgttggtt gctttcgttt tggggatctt atcgacttat gttttatggc
     1381 ttgaaccatt tgccgtagct ttggttgctt tttccagcag attcgtgttg cgattattcc
     1441 atatttctaa accaggtggc ttgttttttg cgatgctttc tgccatggga acgagcatgc
     1501 agctgccaat cgctcaatta ccgatcgtct cactttattt ttttatgggt gtcgtttttg
     1561 ccctgattgc ggcagtgatt acaaaattac tggattcacg gcctgagcag acaattgaaa
     1621 aagcaacttt aaaagaaaga tttcacgaag agcctttagt gatcattgat tctgttttct
     1681 acagcgctgc cttatttctt tccgtttatg tcagtcacgg cttgaatctg cataatcctt
     1741 attggttgac cttgtcatgt gcatcgattt tgctagcaga aaacttggat gcgatgaagc
     1801 atcgacaagt ccaatatctg attggatcga tgggtgggtt atgtgtgtcg gcattcttgt
     1861 catttgtgcc gtttacgcag ttgcagacca tttttctgat cacttttcta tatggaatcg
     1921 cacaatttct agttgctaga aattatgcag ttgctaatat ttttcttaat ccgatggcgt
     1981 tgatgctatc tacacttatt cgtggagcat acttgatttc tttgatcgaa tatcgttttt
     2041 tagggatcgt cataggaagt ttcatcggtt taggtgttgc gtgggtaatg acggttggtt
     2101 tgcaacatta tttaacggtt gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc
     2161 aaaagaggtt atgacaagat ttttgtcaca acctctaatt caaggtgttc aaaagccaga
     2221 ttctcagcat taagtcaaat tcacacaaaa atgtggaaag gaactttcaa aaaaatttta
     2281 tatttattct atcaaaaaat tttgtttatg atggtaactg aataaacttt cctgagatgg
     2341 tcaatgcttt ccttgaaagt cttacagata ttctttcttc atagaaagtt taagttttat
     2401 agttagtgta caataaaagt atgtttatac catatggtgt ccggataatc agtcatttaa
     2461 acaatgaggt tcaaagatat gaaaagggga aatttgatga agaaaataag caatgaaatc
     2521 caaattctgg cagaaacttt aactcttccc aaagtcgtta tagagcaaat gattgtacaa
     2581 gcaaaaaaca tccttgatga ggtgtttgag caatatagtc atgaactaat ggatcctaat
     2641 aaagcaaagg cgactgcaaa gaaaatagac aaagtctatg aagaggaacc ttttgcagca
     2701 ttagctattt atctatatgc agcttctcaa agttggaaaa aaatttatca accattgaag
     2761 attccaagaa ctatctattt ggcaacaatg aatgctttta ctagatttat tcatgaacat
     2821 ttccaagtaa caggaagcta tcgctttgac cgtggttttt ggatttggca ttacactagt
     2881 ggcttgatct ttcggattgg tgaattggag tacgaaatga catattttcc gaaagggtac
     2941 acggatctaa gattagaagg aaagcagtgc ctttctcttc atattccttc cgatgcagat
     3001 ttatctcaga ataagataag agatagttac gaaagtgcca ttcgtttttt tgaactttat
     3061 tttcctgatt accattacca agtgatgtac acagatactt ggttactatc acccaatctt
     3121 actaaatggt tgaaaaaaga gtcaaaaatc tgtctttttg cagccgatta tcgattatta
     3181 tcagttgatg aacaggacga tagtggagtc ccttggatct ttggacgagt agatgcccaa
     3241 atacatgatt atccagaaag tacgagttta cagcgacaag caaaagagca attgcttgca
     3301 ggcgagcaca ttggctctgg cttaggtatt cttacttcat aagtttagta cttcgaaata
     3361 gtttaaaaga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
     3421 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa
     3481 agcaattttc gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
     3541 ttttagtgtt ctatcgaaca cacagtacgt tcaataggta aactagctat aacaatagat
     3601 attaaagtta acggacaaga gggccttttt taggtagcta aaacgtaata atgaaaattt
     3661 atttccagag ataggagtca atcagctagt ctgatcgact gttttaacaa gttgactttc
     3721 attttaactt ttgataccat ttaaaacaca aatcgtaatt gtttcgattt gtgtttttta
     3781 gcatgactac aatcaaagaa gaaagtgggt cgtccatatg gaaaaaacag atatctcaag
     3841 tgcataccgc cgcttgaaaa gcccaaatat caaaacacga aaacgtgcgc tgaagatcat
     3901 caaagatgtg aaacggaata gtgggaaaag gtaagaagaa ggaaacttaa ttaaaaaaat
     3961 agccgaaaaa cgtttttcgg ctattttcaa taatcggttt ttgtgtaagg tttccgaaag
     4021 aactgctgtt gattttccat ttttaacata tgataaattg gtagaatctt tcttttcaaa
     4081 ttgataccat tccctaattc cagttatata gttaactaca gaaccacaaa gcacgtacgt
     4141 ttataaaagt attatctgta gttatttttc aattcccttt caacctctta ttttgaccag
     4201 tagtattgat cactttacct tttaacacct ttgttcgttt ttgaaacatt tttagtcaag
     4261 aatggaaacg taactacata atcaaaggtt ttgcgaaata tttcatgaaa tatttcatgt
     4321 agaagcgagc aatacggttg caaataacca ttatcgttta ataagcagtt acgaaagctc
     4381 ccaatatctc taacgttcag ctcactccat ttcttttctt caaaaaaatc agtgattcgt
     4441 atctctttct atttgtcgaa aaagttaact ttaaaaagta acgccgaaaa aatatgcaaa
     4501 aaaataaaaa agcagttact attgctagaa taacatcaag gaataataag ttttggtttt
     4561 cagataatag tcgataagtt gtgtattgga attttagata cagtaataaa gtatttttca
     4621 tgaaattttg tgagaatcac tattacaagt atctttaata aaattaatgg ttaaggagga
     4681 acttaaagta gaatgtaaat gaatagttgt agtcagtcga ttgtgatact agactcaaag
     4741 ttgcaggaat aagtttttca atttgtgatt ggaagataaa aaatgaaaaa gaagggcttt
     4801 tttttatact ttgttgattg tagtgtttat tttccctctg tttagccaag taacattttc
     4861 tatgactcaa ccagtttcaa gtgaggaaca tgataacctt tttcgtgcta cagaagagtt
     4921 taagaaatat caggaaaata ttgttccgga gactccatta gctagtgagc caattactga
     4981 tattgatgga aaccaaatag gatggtatat acagtacgaa gttatggtgg taaataatga
     5041 ctcaatagta aaaaatacag taggcttaga gctaacaccg attgaaaagg tgagttcatt
     5101 gttaacgtat atttatactg atgaaagtga cgagactgag atcattttaa cggattactc
     5161 aaaaattgct actgataaaa agtatatatt gtggatgtag acactagtga aatactttat
     5221 gatatagaaa ttgatgaaaa tttactagat gtagcacaag aaatcgatca tcaaaagcag
     5281 gaagctattc gatatgctgg tagaaaaggg aaaagaaaat atttattacg tgaatgtagc
     5341 aaaagttaga gaagatgaaa atgagtggaa agaattcaag agtcgctact caattaactc
     5401 gactccgaca tttacagttt acagagaagg atcgatagaa aaaactgttt tttggactaa
     5461 agaaagtgga atgtctttag ctgaagtaga agagtttttg gattatgtta gtatgcaaca
     5521 gtaagggagt tcttattatg aaaaattttg acacatatta tgttattgtt ttttcgatat
     5581 ctttgctgtt aatattacta actttaatgc agtataaaga gtatccagag tattttattt
     5641 taacttttgt atatttagta agtagctaca cacttctata taaattcaga agttcttcta
     5701 gagtttctaa agtttgtttc ttaattagct tattgattct ataatatttt tgatttatat
     5761 aggaaattaa aaatattata gaatcaacac tcagagaacg ggttggtttt taatagattt
     5821 aataagtact gataatgaaa tttctcctgg ttgggaaaac gaagttggtt accctgtaga
     5881 agaatttttt caagattact ttgcggagta tgccgatagg ataatgagag attacgtaac
     5941 agataaaact caagaagtca tcaatcaatc ccagtcatta aatgaattat ctgataaggc
     6001 gaaagaagaa gcagttgcca ttatttcttc agggattagt gactatattg atatgagtgg
     6061 tgaaatcaat tatggcgata aagacaaaat tgtacgcatg ataaaagctt ttatcaaaac
     6121 ttttgatgtg aaggcagatc ttaagtttaa tgatacttac ctagtaggaa aattaattaa
     6181 tatccttggg acagagaaag atacggaaac gtttatttcc tctttgtcta tgttgtttac
     6241 agataagcat ttttcaactc agcttggtgg agaggaacta gtggaaatct tttaaccatt
     6301 acgcccagca ttaataaaag tatatcaaaa aagtactcct gatgattttt aagattggtt
     6361 tgagaaataa tgtgaaaaca ctatttttaa tgttaattat agatagagat gaacgcgtca
     6421 tatggttaaa agaatgaaaa agtcaaagat acactgctat atttcactaa ggatatagca
     6481 gtgtatcttt catgtcgttt cactggcagt tttttataaa aggaggtgtg catttcagtt
     6541 gcttttcttt ggtagttagt tgaatcgttt aagaattaac ggatgtggca gacaggtgct
     6601 ctgtccaaaa accagtaaaa tctttgatgg ttccatcttt atttagctgt gcctgttcaa
     6661 atactgtttt gtttttaccg aatggatcaa ttgcaataat tgttccgatc cccgcacgtt
     6721 tggcagcaag taatcctgag taagcatctt cgataacaag acaatcagcc ggagctaagc
     6781 tcagtttgtc tgccgcttta agaaaaatat ccggttgcgg tttcccaggg aaagagccgt
     6841 cgtcatagac aatcttgtcc cagtcaaacc agcgggctaa atcgaaaata tcaaaataaa
     6901 aagcaacatt ctctttgaca gtagccgtgg caatcgttaa tggaatctgt tggtcagtca
     6961 gctggtcaag agtcgcttca agcccttttg tcaactgaag ctcgttttca ttttgcaagc
     7021 aaagcatacg ataatagctt tctttttctt ctgacaatac ggcgatctca gaggcggtca
     7081 aatgctctga aatgaaatgt ttcaaaatct ccgtattcgt ccgaccatga atatttttta
     7141 aaatatcctc gtctgataaa ccatcttttg tatgatcatg tatcatatgc agccaagcag
     7201 cttcatgcag atgagagtcc aaaaacattg ttccgttgaa atcaaatata atccctttca
     7261 tagttacgct cctatagtgg tattttcggt tgaatcagca atttgcagat cgacatattg
     7321 tgcatagtgc tcttgaacct cccgcgagat ctggcgatcc gtaatgagca gatcgaaatc
     7381 atgcaaatta taaaacacat aaaaatcatc tcgattgaat tttttattat caacgagtag
     7441 atatttctta cgggaattat ttagagcgat ctgctgtgct tctccttctt cggtactgta
     7501 agtagagatc gcttcgttgt gaacgccatt tgaactgata aatgcttttg taaactttag
     7561 tcttcttaaa ttgtcgttgg tgatcggtcc gacaaatgcg ccggtatttt tccggaaatc
     7621 accgccaatc agtagaattt ctggtccatc tgcttgagca agaatatcaa aaactggata
     7681 attatttgta atgatccgaa ttttcttgtt cagtaaatgc gtcgccaata gttcgattgt
     7741 tgtacctggt cctaagaaaa tcgtttctcc ctcgtgaatc aaggaggctg ctgtggcagc
     7801 aatttctttt ttttcttgaa tctgcagagt ggctttttca gtgtgagaaa gttcttgatc
     7861 taaagtgtag gaaagacttt gagcccctcc atggacacgc agcagcttgc ccgatttttc
     7921 taattcatct aaatctctgc gaacggtcat atcagaaaca tctaattgat tgatgatttc
     7981 attaacggta aggattcctt ttttattgac catttctacg atagtaagca atctttcttt
     8041 tttcaacatt gtcttcacct ctaatttaag gataatctat catataaacg gacaacagtc
     8101 aatctaattc gaacaaaagt aaacatttag ggtggttttt atgtttgtta caatagattt
     8161 tattgctgtt ttctggtttt tatgattatt tgtgtttgtt tttgttgaca aatgttttgg
     8221 atgcgtttac aatgcaatca gttagaaaga gcgaaaactg attatagatt tgaggagaga
     8281 tgtacatgct taaattacca gaagatttta tttttggcgg agccacagca gcctatcagg
     8341 ttgaaggagc cactaaggaa ggtggcaaag gcgctgttgc ttgggatgac tttttagagg
     8401 aacaagggcg attcagtccc gatccggcaa gtgattttta tcatcaatat gcaaaggaca
     8461 ttgaattgtg tgaacgtttt ggtgtaaatg gtctaagatt atccattgct tggagccgca
     8521 tttttcctga tggagctggg aaaccaaatc cagaaggaat cgctttttat catcgtgtgt
     8581 tcgaagaatg taagaaaaga aacgtaacgc cgtttgtgac gttgcatcat tttgataccc
     8641 ctaaacgttt gtttgaccat ggagattttt tgaatcgaga aacaatagaa gcatttgttt
     8701 cctatgccat tttttgtttt catgaattca aagaagtcaa ggtgtggagt acctttaatg
     8761 aaatctatcc agttgcaacc aatcaatatt tgttaggtgt tttcccacca ggaatcaaat
     8821 atgattttac aaagatcgtc gcctgtcttc ataatatgat ggttgctcat gcccgtgtag
     8881 tgaactattt caaagaaaac gaacttccgg gtgaaattgg tgtagttcac tcattagaga
     8941 cgaaatatgc ggcaacagac gcgccggaag ataagcatgc tgccttttta gacgatgcat
     9001 tgtcgatccg tttcttatta gatgctacct atttaggcta ttattcaact gaaaccttga
     9061 ctgctcttga tgagatatgt gaagcaaatc aggccagcta tcattttcct gaagaagatt
     9121 tcgttgaatt gaaaaaagca tccacgcgta atgactactt agggatcaat cactaccaat
     9181 gccattttgt caaagcttac gatggagaaa atgcaatcca tcataacggt acgggtgaaa
     9241 aagggacctc tgtttataaa gtgaagggaa tcggagaacg tatttataaa gaaggaattc
     9301 ctcgaactga ttgggactgg ctgatttatc cagaaggtct atatgacttg ttgctgcgaa
     9361 ttaaaagcga ttacccacat tacaacaaaa tctatatcac tgagaacggg atggggtata
     9421 aagatcagtt tgaagatggc attattatgg atcagccgag aatcgactat ttgagagtct
     9481 atttggaatc cttaagcaag gcgataacag ccggggtaaa tgtgaagggg tattttttgt
     9541 ggtcgcttat ggatcttttc tcatggacaa atgggtataa caaaaggtat ggcttgtttt
     9601 atgttgattt tgaaacccag aagcgttacc caaaagaatc agcttactgg tacaagttgg
     9661 tcagtgaaac caaaacgatt atttaataag tgataatgga ggaagaaaag atgaataaag
     9721 aggaattaca aatgcttggt tttgagatcg ttgcatattc aggcgacgct cgcagtacat
     9781 tattgaaatt attgaaagaa gtaagacaag gaaactttga acatgttgat tcggcgttga
     9841 aagaagccga cgaaaattta acccttgcac ataattcgca aacaaaaatt ttagcggaag
     9901 aagcatctgg gaaagagatg gagatgggat tcatttttat ccatgggcag gatcatttaa
     9961 tgaccacttt attgctgaga gatctgattc aagactttat tgtactgtac agacagaatc
    10021 aaggatagga gaagaacgct atggatggaa tcattaaaca aatcgaaaaa gggaaaccat
    10081 tttttgaaaa aatctcacgt aatagttatt taggagccgt tcgtgacgga tttttaacag
    10141 cgatgccagc tattttattt tcaagcattt tcattttgat tgctgcggtt cctgagattt
    10201 ttggttacca atggcctgaa tctgtctcca catggctatg gaaagtctat aattattcga
    10261 tgggtgttgt agggttactc gtttcagcaa ccactgctcg ctgcttggct gattcgatga
    10321 atcgtcgctt accgaaaaac aaagtaatca acaccacctc tgtgatgctg gcctctgtgg
    10381 taagtttcct gttgttaagc gtctctccga ttgaaggtgg attttcaacg gaatatatgg
    10441 ggacaaaggg actactggct tcctttgtcg ctgcattttt aacagttaat ttatataagt
    10501 tttgtgtgct gaaagatatt accattaaaa tgcctaaaga agttcctgga acgatttcgc
    10561 aaaccttccg agatattttt cctttctctt tttcagtatt tgcggccgtt ttaattgatt
    10621 taggcattcg acaaatcttc caaatctcat ttgcagaagc aatcattacg gtgctgcagc
    10681 cgctatttac agcagctgac gggtacttag gaatcgctat tatctgggga gcgatggcgc
    10741 tgttttggtt tgtcggtgtt catggaccgt cgatcgtgga gccggcaatt gcggcaatta
    10801 tctatgccaa tgtggaaacc aatctgcaat tgttcaattc cggtgaacaa gcttctaaag
    10861 tactgacggt gggtttaggg aattttgtag gaactatggg aggaactggg gcaacattgg
    10921 ttgttccttt tatcttccta ctctttgcta agtctaagca gttgaaggca gttgggaaag
    10981 cttcctttat tccagtaagt ttcgctgtca acgaaccatt gctatttgcg gcacccatga
    11041 ttttgaaccc ttattttttt attccatttt tgttaacacc tgttgtaaac gtttgcctgt
    11101 tcaagttttt tgtcgatgta ttgggcatga acagctttat gtacgttttg ccttgggcaa
    11161 ctccagcacc gatcggtttg gttctaggaa cgggcatggg tatcttagct tttgttctag
    11221 cagcggttct aatcgtcgtt gacttcctga tctatctacc attttgcaaa gcatacgatg
    11281 ctgtcttagt agagaaagaa gcacaaaaag cagaggtgat cgcaaccgag gaaggtgctt
    11341 ctgccgatgt acgtgaggaa gtacctgtgg cagcgagtca agttatcagc gatgcaaata
    11401 aagaaatcaa tgtgttggta ctttgtgctg gcgcagggac aagtgcgatg ttagctaatg
    11461 ctttaaaaaa aggtgcatct gaatcgaaag caccgatcag tgcaatggcg ggtgcttatg
    11521 gttctcatta cgaaatgctg caggattttg atatggtggt cttagcaccg caagtcaatt
    11581 cttactatga tgatatcaaa gaagacaccg atcgcttagg aatcaagctg gttgcgacaa
    11641 aaggagcaga atatatcgga ttaacgagag atcctgaaaa agctgttcag tttattctga
    11701 accaatttca ataaaggagg agccataatg aaaatgggaa tttggttgcg taaagctgtt
    11761 ttatttgcag ctgtgttagg ggctttgggg gctgtttcaa aaactgttga agcatctgcc
    11821 agcgaattta ttcgcggagc agatatttcg atcctgtcag atatggaaaa atctggtgca
    11881 aaatattatg aagacgatat ttcaaaagat gccttagaga ttttaaaaaa taatggtgtc
    11941 aactatgttc gactgcgttt gtggcaggat ccttataata gtaatggaga aagttatgga
    12001 gcagggacaa atgatctgga aacgacgatt gaccttgcaa aacgagcaaa aaatctggga
    12061 cttaaggtgc tgctggattt tcactacagt gatttttggg ttgatcctgg gaaacagaat
    12121 cttcctaaag catggcaagg tctgaccttt gaagaaatga atacggcatt gtacgactat
    12181 acaaagaacg ttctgtcgga aatgaagcag ctggacgttt atcctgatat ggttcagatc
    12241 ggcaatgaac tgaatagcgg aatgctctgg ccttatggca agtcttgggg agaaggcggg
    12301 ggagaatttg atcgattggc tgcttttttg aagtctggta tccaagctgt tcgagacact
    12361 caacctaaaa caactccagt aatgctccac ttagctgatg gtggcgatac aggagcattt
    12421 acttggtggt ttgatgaaat cacgagtcga ggtgtgtcat ttgatttaat tggagtgtcg
    12481 tattatccgt attggcacgg ttcaatgaat gaacttcaaa ccaatatgaa tgcgatcagc
    12541 caacggtatg gtaaagaagt gatcgtagtt gaaacagctt atgggcatac aacagcgaat
    12601 gccgatacta tgccaaatgc ttttggtgaa gccgaagcag ctgcgggggg atatgaacca
    12661 acaccgacag gacaagcaga gtatttgctg gatcttgccg atcggattca ggcagtaccg
    12721 aataatcgcg gggcgggctt cttctattgg gaacctcttt ggtacaacgg aaatgtgagt
    12781 tgggcaacac aagcaggtat gaattatctc agtgttcaat ccactatggg aaacgaatgg
    12841 gacaaccaag cgatgtttga ttttgctgga aacgccttgc caagcttgcg tgcgtttaaa
    12901 caagcagggg cgcaaataaa tctagttaaa aatgcaagtt tcgaaagcga tggaccaacg
    12961 accagtcctt ccaattggca aacctggttt caacaaggaa ccgctaaggg tacgatcaaa
    13021 acggaagcgg gcactttttt tggggactac aaactaactt tttggcagga tcaagcatac
    13081 gaggcatcag cttatcaaaa tgttagtggc ataagcaaag gaacttacac attgagtgcc
    13141 tatgtaatgt ctgggggaga gtttgatact aatcagttat atatcaaaaa ttatggtgga
    13201 gcggaactaa atactgcggt tgttaaaaca agtgaatgga caaaaattac aatcaaagac
    13261 attccaatta caacaggtgt ttgtgaaatt ggggtttata gcaaggcttc aggaaatgct
    13321 tggacgaacg ttgattttgt ttcattggtg aaacaataga ctgcggatcg tttcgattca
    13381 agatcggagg ttcccttcag ccacatcgtt agcgtagaag gcaggaatcg aacctgcacc
    13441 tcgcctttgc caaaaataac cagaaacgat ttccagtcat ttttaccaaa acgagtaaag
    13501 caccagactt catctacact ttgtattatg ctaaagagag ttcaacgatt tcgtaagaag
    13561 caatgaaaaa attgcaaaaa gttgtgcaga taacataaag tttgtttaag aaagggtaac
    13621 cttagaattc cgtcgtgaat atgatttgag cacttagatg gatagaaaaa agttctcgga
    13681 aaacagttcg tttttcgaga acttcttttt atgataagaa tgaaacaaat taacttatat
    13741 tatagtctct tttggtaggg aaccatctta tttttaatga aaatcaagca ttttagctat
    13801 tgaagaatgt tgatttatag ggattccccc tccatattat gttggctctt tattcccact
    13861 ccactgttgc tggtggttta gaagtgatgt catacacgat acggttgacg tgggctactt
    13921 cgtttacgat acgtacagag attttttgca agacttccca tgggatacgg gcaaagtcag
    13981 cggtcattcc gtcgattgaa gttacagcac ggataccgac tgtgtagtcg taagtacgtc
    14041 cgtcacccat aacaccgaca gagcggatcc ctggaagaac agtgaaatat tgccagatat
    14101 cacggtcaag tccagcagct gcgatttctt cacgcaagat agcatcagat tcgcgtacga
    14161 tttctagctt gtcttcagtg atttcgccaa gaacacgaat accaagacca ggaccaggga
    14221 atggctgacg ccaaacgatt gaatcaggca tgcctaactg agtccctaat tcacggactt
    14281 cgtctttgaa taatgtgttt aatggttcga tcaattcaaa ttgcatatct tctggcagtc
    14341 cacctacatt atggtgagat ttgatcgtct gtgctgtttc tgtaccagat tcgataacgt
    14401 cagtatacaa cgttccttgt gctaggaaag agacgccttc ttcaccagca agttttgttg
    14461 cttcgtcatc aaaaacatat acgaattcat tcccaatgat tttacgtttt tgttctgggt
    14521 cagatacacc tttcaacttg tctaagaagc gatctcttgc attgactttg atgatgttca
    14581 agccgaattt tccgcctaga ctttccatta cttgttccgc ttcgcctttg cgtagcagac
    14641 cgtggtcaac aaagatagat gtcaattggt caccgatcgc tttttgtaaa agaaccccaa
    14701 cgacactaga atcaacgcca cctgaaagac caagcaagac ttttttgtca ccaacttgtt
    14761 cacggatttt ttctacttgc atatcgataa agttatccat tgtccagtta ccttcacagt
    14821 gacaaacatc aaaggcaaag tggcgcagca attcgattcc gtattcggaa tgacgtactt
    14881 ctgcatggaa ttggatacca taaaaattac gtgttgtgtc ttggatagaa gcaatcggac
    14941 aatctttgct tgttccgact gtttcaaagc cttcaggaac tttagttacc aagtcaccat
    15001 ggctcatcca tactgtttgt tgttttggtg tttctttaaa caagacagct tgatcgcttg
    15061 ttacttctaa caaggctttc ccatattcgc ggtttttagc aggctctacg gaaccgccaa
    15121 gtttgtacgt gatcaattgc ataccatagc aaatccctaa tacaggaata cctaaattga
    15181 agatctcagg atcgatccca aatgcattct catcatatac actgtttggt ccgccagaaa
    15241 gaatgattcc ctttggattc atcgctttta cttcttctgc agtaatgcgg tggctcaata
    15301 attcggaaaa gactccaaga tcacgaatac gtcttgtgat caattggtta tactgactac
    15361 cgtagtctaa tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt gtcaaattta
    15421 ttacccctat ccctttaaat tttatctcaa aatagtcaaa ctattttaaa atgctaatat
    15481 tttcgcatga aaatcgaatc gatgatatga ttttcagttt tatggagaat tatatcggct
    15541 cttcctcgtg taggcaagat atattcctgt aaatttttca aattcacatt tttccataca
    15601 tcttttgcca ttttaaaggc ttcttcacga tctccgatag caaaacgata ataataattc
    15661 tctggatttt ggaaagccgt atccagtaac gcaccaaaac gatccaaata ccatttttcg
    15721 atcagcacgg gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct gacgtagatt
    15781 tgctgattcg caggtaattg aagcgtattg atcccctcaa cgatcaagat atcaggctgc
    15841 tggatcaatt cgtacttgtc tggaatgata tcatacacat catgtgagta aacaggtact
    15901 tttatctctt cctgaccaga ttttacacgg ttcaagaaat cgatcagcat ctccatatca
    15961 taactttcag gaaatccttt acggtccatg attccacgct cttttaatat acgatttgga
    16021 taaagaaatc cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt acgagacaaa
    16081 atcgtctgca gcagtctggc tgttgtactt tttccaacgg ctacacttcc ggcgatcccg
    16141 ataataaaag gcggtacttc tacgtattta tgaagaaata aacctttact caagaacaaa
    16201 gattcaaatt ctttcatata aagataaatc agatgagcaa gaggtacata gatatctcga
    16261 acatcctgca tggaaatgcg gtcattcaaa cttttgatat tatctagttc atcttgtgtc
    16321 aaaggtgctt gcccattacg ataaaattct cgccattctt ctcttgccac acggtagtag
    16381 ttcattggat cattcatatt agcctcctgc gttctcccat caatggaact attttaacac
    16441 aaaataaacg caaaccccac cacttaagag agattgccga tctttttgta taatctttat
    16501 aatagaaaga aacggagtct tattcttgac ttgcgcacgg atacataaaa ggatagactg
    16561 ttgtcatgaa agaaatgaga aggatggaag acatgacaaa tcattactat tctgaacgcc
    16621 cagatacggc gcacgatttt gaacaatggg cttttgagtt aaaaggcaga acctttcaat
    16681 ttgtaacaga ttctggtgta ttttcacgtg aaacagtgga ttatggttca cgtgtcctga
    16741 ttgatgcttt cgattgggaa gagttacctg atgaaggaaa gatcttagat gtgggatgcg
    16801 ggtatggacc gattgggtta gctttggcat ttgcgacaca gcggtttgtt gaaatggtcg
    16861 acattaatga acgagcggta agtctagcgc aaggaaacgc aaagcgtaac caaatcgaac
    16921 atgttgatat ccatcaatcg aatatctatg aagcagttca tgaagaaacc tatgcagcta
    16981 tcgtaagcaa tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt ttgacaggcg
    17041 catatcctcg gttgaagaag ggcggtacac tgacaattgt gatccagaag aaacaaggag
    17101 cgccgagtgc gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc gtcacaaaag
    17161 ataaaggata ttatatcatc aaaagtgtga aagaataact agctgaaaaa tagtggagcg
    17221 acgaaagagt ggaactgggc gtgcatgtac gggtacgtat cagttctttt tttgttgaga
    17281 agtttttaaa aacaaagaac tattttttct tcaaaaagaa atagtaggaa cagagtcgtt
    17341 gttttttacg ataaaataaa taagatacag aacttgtata gaaggctgcc aagtcggtag
    17401 ataaaggagg tttttatcaa aaaacatact ttcctttttt ctctattctt ttcttggggg
    17461 ttttggtaca ctgataggga atttagatag aagggagcca ataggatgaa gctgaccatc
    17521 agagatatcg cagaaatggc aggcgtatca gtaacaactg tctcacagat tttgaataac
    17581 aaaggaagca gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat
    17641 cattataagc ccgattattt cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga
    17701 atgatcgtcc ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg tgtagagagc
    17761 tatttgaatc ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat
    17821 gagatcaagt atttacaaga gctttcccac cgctcagtgg acggaatctt gctggccaca
    17881 ccaaatattt tacctgagaa atatgcatta gacagcggtt tttatcaaaa aatgccaatc
    17941 attttaatcg atcgcggatt gaatcgtcgg gataacggac ggcttatcgt aaaggaatat
    18001 gagggcgcct atcaagctgt ttcgctgttt attgaaaatg gccatacgaa gatcggcatg
    18061 ctgaaggaaa ccactggata ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg
    18121 ctgaaagatc atggcctgcc ttttaatggg aaatttgttg aacatggaga gttgactgtc
    18181 caaggtggat atactgcatc aaaaaaatta ctcaaacata aagatatcac ggctattttt
    18241 tgcgggaatg atgccatggc gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa
    18301 atcccagaag atatctcagt cataggattc gatggattaa agctgtcaga atacatgatt
    18361 ccgcaattga cgacagtgca gcagccaagt tttgatattg gattttatgc tgcacgtttc
    18421 ctgatcgata cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa
    18481 ttaattattc gtgagagcgt aaaagtattg acaaagggct aactcggcgg tatattgctt
    18541 atgtaagcat ttacaaaggc tagttagtgc tttttttatt tttagtaaac cagtttacta
    18601 aagtggttta cctttcctaa aaataatgta taataccgaa tgaagacaag gaggatcagg
    18661 caatgcgaat ggtggatctg attgaaaaaa agagggatgg aaacgagctg tcgaaagagg
    18721 aaatcgagta catcgtcacg aactatacga atggaaagat tccagattat caagtcagtg
    18781 cattattgat ggcaattttt tatcaagaca tgacaaatga agaaatcacg aacttaacat
    18841 tggcgattgc taattctggt gatgtcatcg atttgagttc tcttgaagga ataaaggtag
    18901 ataagcattc gacaggtggc gttggtgaca cgaccacatt gattttagcc cccctagtag
    18961 ctagtgtcgg agtgacagtg gctaaaatgt ctggacgcgg tctaggttac acaggtggta
    19021 cactagacaa gctagaagca attcccggat ttcagatcga gctttcggat gaagaatttg
    19081 tacgcattgt taatgaaagt aaagtagcgg taattggtca atcaggaaac ttggcaccgg
    19141 cggacaaaaa attgtatgcc ttgcgtgacg tcacagcgac cgttgattct cttccgctga
    19201 ttgccagttc gattatgagt aaaaaaatcg cggcaggagc agatgcaatc gttttggatg
    19261 tgaccactgg agacggtgct ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga
    19321 ccatgacgag cattgggaaa ctggctaacc gagaaacagt ggcggttatc tcagatatgt
    19381 cagaacctct tggcgaagct atcggaaata gtctagaagt tgtcgaggca atcgaaacat
    19441 tacaaggaaa tggtccagaa gatctagtag aaatgtgcta cgccttagga agccaaatgg
    19501 ttgttcttgc tggcaaagct aagacaaccg atgaagcaag aacgttgctt caagaagctc
    19561 tggaatcagg taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa gggggagacc
    19621 cagcgatcgt ggaacatcca gaacgaattt tgactgcacg ctacacaatg gagcttcctg
    19681 ctaaacaaag cggagttgtg tcaaaaatcg tggcaaatga attaggaatt gctgcaatga
    19741 tgctaggtgc tgggcgtaaa accaaagaag aagatattga tcacgctgtt ggattgaaac
    19801 tgcataaaaa aattggtgac actgtgacaa aaggtgagtc actattgacg atctatagta
    19861 acgacgaaga aatcacttct gtcattgaat tattatataa aaatattgaa attggtgaat
    19921 cggcaatgaa gccaacgttg attcatgaca tcatcacaga ataaaggaaa aaagcggact
    19981 ttaccgtttt aaagaaaagg ggaaaagaaa tggaactaaa ccgaatgatc gaccatacta
    20041 ttttgaaagc agacgcatct aaagaagctg tgatgcaaat tatcgaagaa gcaaaaaaat
    20101 accattttta ttctgtctgc atcaatccag catgggttgc gctagcgaaa gaacaattac
    20161 aaggtgagcc agtagccgta tgtacagtaa tcgggtttcc tctaggagca aacacacccg
    20221 agacaaaagc atttgaaaca acagatgcca tcaataacgg tgcagatgaa gtcgatatgg
    20281 tcatcaatat cggtgctttg aaatcaaagg atgatcaaca agtccaaaaa gatatcgaag
    20341 cagttgttga agcagcaaaa gatcgtgcgc ttgtcaaagt tattattgaa acatcgttat
    20401 tagatagaga agaaatcatc cgagcttgcg aaatagcaaa agctgcaggc gctgattttg
    20461 tcaaaacatc aactggtttc tcaacaggcg gagcaaaggt agaggatgtc cgtttgatgc
    20521 gtgaaactgt aggaccagaa atgggtgtca aagcttccgg cggtatccat aacgaagaag
    20581 aagcaatggc aatgatcgaa gctggtgcta caagaatcgg aacaagcgca ggagtagcaa
    20641 tcgtatctgg ttcaactggt gaaggctatt aattttttca aaggagaagc tcatggcaga
    20701 actcaaacaa gaatggatca acacagctat tgaagcgtta gataaagcct atgttcctta
    20761 ttcccatttt ccggtaggcg cttgtctcgt aacggaatca ggcaagattt atcaaggcat
    20821 caacattgag aatgcatcat ttggattaac gaattgtgcc gagcgcacag cttttttcaa
    20881 agcagtatct gaaggagaaa aaacatttac gcatctagtc gtagcggggc acacccccga
    20941 tcctatctct ccttgtggtg catgtcgaca agtaatggca gaattctgtt cccctgatat
    21001 gccggttacg ctagtaggag acaatggtgt gacgaaggca acgaccgttc gtgcactgct
    21061 gccttatgca tttactgaaa aagatctata actcatgttc attcagtcag ctgactgttg
    21121 aaaaataaaa caattatttt aggaggcttt acagaaatga aaaaagcaaa attatttggt
    21181 ttaggtgctg tcgcactagc agcagggtta tttttaggtg catgcggcaa caacggctct
    21241 actgattcat ccggtggcaa aagctcatca gacacaacaa ctgcagcatt gatcacagac
    21301 acaggtggtg tagacgaccg ttcattcaac cagtcagctt gggaaggact agaaaaatgg
    21361 gggaaagatc aaggactttc acgaggaaat gatggcttcc aatatttcca atcttcaaat
    21421 gaatcagatt acatcccaaa tattgaccaa gcattgaatg ctggattcaa aacgattttt
    21481 ggtattggtt ataaactaaa accagcgatc caagaacaag caacaaacaa tacaggtacg
    21541 aactttgtta ttatcgatga tgtcatcgat ggattagata acgttgtatc tgcaactttc
    21601 aaagacaacg aagcttctta tcttgcaggt gttgcagcag cttatacgac tgaaacaaac
    21661 gtagtcggct ttatcggcgg tgtaaaaggt gaagtaatcg atcgttttga tgctggattc
    21721 aaagccggtg ttgatgcagg tgcgaaagaa ttaggaaaag aaatcaaagt attgaatcaa
    21781 tatgctggtg acttctctgc tccagataaa ggacgttcaa ttgctcaagg tatgtatgca
    21841 caaaacgcag atatcatctt ccatgcttca ggcggtacag gtaacggtgt attccaagaa
    21901 gcaaaatcat tgaacgaatc aggcgacaaa aaagtttggg ttatcggtgt tgaccgcgac
    21961 caatcagacg aaggcgaata cacattaaac ggcgaaaaga aaaacttcac attgacatct
    22021 acactgaaag cagttggaac agtagtagaa gatttggcac aaaaatcagc ggacggtaaa
    22081 ttccctggcg gcgaacacac tgtttatgga ttgaaagaag acggtgttgg tctaacagaa
    22141 ggacaacttt ctgacgaagc gaaaaaagca gttgatgaag caaaagaaaa aatcatttca
    22201 ggtgacgtaa aagttccaga aactcctgaa gaaaactaaa accatttcta tagccttgtt
    22261 agacacatgt ttgacaaggc tatggtttga ttagaacaag aattgttcat ttatccaagc
    22321 tgccttgtga cagtttggat aagggaacaa ctccctgaag taatgtttgt ttcaaaagaa
    22381 gaacgctcac taagcgtgaa tcttagtcaa tcgttgccct agatagaaat cgttaggatg
    22441 tgaaaaaaag gtggttcaag aaaatattgt catagaaatg cagaatatta ccaaagaatt
    22501 tggaacattc aaggcaaatg acaacatcaa tttacaagta aaggcaggag aaattcatgc
    22561 gttgcttgga gaaaacggtg ctggcaaatc tacattgatg aacgtgcttt ccggattatt
    22621 agagccgaca tcagggaaaa ttttgatgcg tgggaaagaa gtaaagatca caagtccgac
    22681 caaagccaac caattaggga ttgggatggt ccatcagcac tttatgcttg ttgatgcctt
    22741 tactgtaaca gaaaacatcg tgttgggaag cgagcctagt cgcgcaggga tgcttgacca
    22801 taaaaaagca cgaaaagaga tccaaaaagt ttctgaacag tatggattgt cagtcaaccc
    22861 agatgcttac gttcgtgata tttcagttgg aatggaacaa cgggtagaaa ttttaaaaac
    22921 actttaccga ggagcagatg tactgatttt tgatgagccg acagctgtat tgacccctca
    22981 agaaattgat gaattaatcg tgatcatgaa ggaattagtc aaagaaggca agtcgatcat
    23041 tttgattacg cataagttag atgaaatcaa agcagtagct gaccgctgta cagttatccg
    23101 ccgtggaaaa ggaatcggta cagtcaacgt taaagacgta acctcacaac aattagccga
    23161 tatgatggtt ggaagaacgg tttcattcaa aacgatgaaa aaagaagcga agcctcaaga
    23221 agtcgttttg tctattgaaa atctagtggt gaaagaaaat cgtggattag aagccgtgaa
    23281 aaacttgaac ttagaggttc gtgctggcga agtacttggt atcgctggaa tcgatggaaa
    23341 cgggcagtcg gagttgatcc aagctttgac tggtttgcga aaggcagaaa gcggacatat
    23401 caagctaaaa ggggaagaca tcactaataa aaaaccccga aaaatcactg aacatggtgt
    23461 aggacacgtg ccagaagacc gtcataaata cgggttagtc ctagatatga cattatctga
    23521 aaatatcgca ctacaaacct atcatcaaaa accttacagt aaaaacggca tgctgaatta
    23581 ttcagtaatg aatgaacatg ccagagaatt gatcgaagaa tatgatgttc gaacaacgaa
    23641 tgaacttgtt cccgcaaaag ctttatcagg cggaaatcag caaaaagcaa ttatcgctcg
    23701 tgaagtcgac cgagatcctg atctgttgat tgttgcaaat ccaactcgtg gactggatgt
    23761 aggtgcgatc gaatttattc ataaacgtct gatcgaacaa agggacaaat acaaagcagt
    23821 gttactgatc agtttcgaat tagaagaaat tttaaatgtt tcggatcgta ttgctgttat
    23881 ccatgaagga gaaattgtcg gtatcgttga tccgaaagaa acatctgaaa atgaattagg
    23941 tctattgatg gcgggctata ccttagaaga agcacgaaaa gagttagaaa agggggctgg
    24001 cgcacatgaa tgatcgcagc gaaaaattcc gtaatttatt agtgcctgta ctttcagttg
    24061 ttttaggatt gatcttaggc gcgatattga tggctgcttt tggttttcag cccatccaag
    24121 gttattcttc tatgctagat gcagcccttg gttcgcaaag agggataggt gaaacgttaa
    24181 ggcaagcaac gccgttgatt tttacagcat taggtttttc tgtagctaac tcagctgggt
    24241 ttttcaatat tgggctttct ggacaagcac tttgcgggtg gatcgtcagt gtctgggtgg
    24301 cacttgcgtt tccagatctg ccaaaagttg ttttgcttcc gttatgcgtg atcttaggtg
    24361 ccttagctgg tgcgatggca gcggctgttc caggtttact tcgagcattt tttggaacaa
    24421 gtgaagtgat cgtaacaatc atgatgaact atatcttatt atacgtaagc acatttgctt
    24481 tacatgaagt gatgcctgaa agctatcgct caagtttgga ctcttccaat atgatcagtc
    24541 aaaatgcctc actgcggtta ccatggttaa cacaagcttt tggcggctct cgcgtaaact
    24601 caggattatt tttagcaatc atcgcgctta ttttcgtttg gctgctgatg aagaaaacga
    24661 ctttaggatt tgaaatccgt tctgttggat tgaatccttt tgcttctgag tatgcaggaa
    24721 tgagtagtaa acgtacgatc gttttgtcga tggtcatttc tggcgctttg gcagggcttg
    24781 gcggtgtggt tgaaggatta ggaacgtatc aaaacttctt tgttcaaacg acttcgctat
    24841 ctattggatt tgacggaatg gctgtttcct tgcttggttc tggatcagca atcggtattc
    24901 ttttatcagc gatcttgttc agtattttga agatcggtgg acttggcatg cagacaggag
    24961 caggtgttcc gtttgagatc gtcaatgtat cgattgcttt gattatcttc tttgtcggga
    25021 tcaacttcct gattcgtttc gtcatggcta aagtcttcca agggaaaaaa caagaagaga
    25081 tcgttgcgac aatagaatcg aaaccaacga atcaaaacca tgaagggggc gaactataat
    25141 gtcgactatc gaattaattg ccttgatcct tacttctaca ctggtttatt caacaccgct
    25201 aattctaacg tcactaggcg gaactttttc tgaacgaagc gggatcgtaa acgtagggct
    25261 agagggaatc atggtcatgg gtgcttttag ttccatcgtt ttcaacttga cgatgagtcc
    25321 atcacttggt tctatgacac catggatcgc tattttagtg ggtggcgtcg taggtatcct
    25381 attttctctg attcacgcag tagcaacagt cagtttgcgt gctgaccata ttatcagcgg
    25441 gacagtaatt aatctgatgg cacctgcttt aggtgttttc ttgatcaaag catggtatgg
    25501 taaaggtcaa actgataaca tcacgcagaa tctggggtat ttttctttcc caggattagc
    25561 agatattccg gtcattggac caattttctt taaaaataca ttcttacctg cttattgtgc
    25621 tattttgatt gctgtcctag cttgggtgat catcttcaag acgaaattcg gcttgcgcct
    25681 ccgttcagtt ggtgaaaatc ctcaagctgc cgacacatta ggtatcaatg tatatgctat
    25741 gcgttattca ggcgtactga tttctggctt gttaggcggt atgggcggtg ctgtttttgc
    25801 tcagtcgatt tccggtaact tttcagctgg tacgatcgtt ggacaagggt tcatctccat
    25861 ggctgctatg atctttggta agtggaatcc tttaggggca atgggtgcct cattgttctt
    25921 tggttttgct caaagcttga gtatcattgg cgcacaatta ccagttattt cttcgattcc
    25981 gccagtattg ctgcaaattg ctccatattt gctgacaatc attgtgctgg ttgttttctt
    26041 agggaaagct tctggtccaa aagcaaacgg gaagaactat atcaaatcaa aataatcttt
    26101 tagcgaataa aaagggtggg ttacgggaag tgtttttcgt ctcgcccttt ttgttttaaa
    26161 ggcaataaaa ataaaaagaa agaaggaata atcatgttta aacgcgtaca tctgatcgtc
    26221 atggattcag taggaattgg agaagcacca gatgccgata agttcggtga tgttggttct
    26281 gatacattag gacacattgc gaaagaagca ggattgaacg ttccacattt agagcagctt
    26341 ggtttaggaa cgatccgtcc tcttgaaggt gtggaatcag tgaaagatca tcaaggctat
    26401 gcgacgaaat tagaagaagt ttctgtaggg aaagatacga tgacaggcca ctgggaaatc
    26461 atgggattga atatcaaaac tccatttcgt gtgtttccta atggattccc tgatgatctg
    26521 atcaaacaaa tcgaagaatt ttctggccgt aaagtcgtat gtaacaaacc ttatagcgga
    26581 acagctgtta ttgacgatta cggtgaacac caaatgaaaa caggcgattt aattgtatat
    26641 acttctgccg atcctgtcct tcagatcgcg gcacatgaag aaatcattcc tttagaagaa
    26701 ttatatcgga tttgccaatt cgttcgtgat ataacaaaag atgatccgta tatgattggc
    26761 cgaatcattg cacgtccgta cgtgggtgaa ccaggagact tcacacgaac aagcaatcgc
    26821 catgattatg cgttgaatcc attcggacac acagtccttg attctttgaa agaagcagga
    26881 aaggacgtga ttgctgttgg aaagatcaac gatattttca acggacaagg aatcactgaa
    26941 tctgtccgca cgaaaagcaa tatggatggc gttgaccagc tgctgaaagt tatgaaaaac
    27001 gatttttcag gaatcagctt taccaattta gtagattttg atgcattata cggtcatcgt
    27061 cgggatgtag tcggctatgc gcatgcgatt gaagaatttg acttacgaat tccagaattg
    27121 cttgaagcga tggaagaaga cgacttgcta atgattacag ccgatcacgg aaacgatccg
    27181 acgtttactg gtaccgatca tacgcgagaa tatgtaccgt tattggttta cagtaaaaaa
    27241 atgaaagaac aaggggcatt gcctcaagga tattattcag atatttcagc aacgattgct
    27301 gagaacttta gtgtttcttc gactgaaaat ggcgaaagct tcttgaaatt attaaaataa
    27361 tctttttaaa taggagaaag caaatgacca agttaagcga gatgttacaa gaaacgaccc
    27421 agtttatcct tgataaagga gtaaaagaaa ttgattttgg tttgattcta ggttcaggat
    27481 taggtgaatt agcagatgaa atcgaagatg ctgtaatgat ttcttatgat gagatcccgt
    27541 tctttcctgt ttcgacagta gttgggcatg caggaaagct agtttatggg tctctggcag
    27601 gcaagaaagt gttagccatg caagggagat tccactacta tgaaggtcat tccatgcaaa
    27661 cagtgaccta tccagtacgt gtgatggccg gactaggggc ccattcagtc attgtgacta
    27721 atgcatgtgg tggtgtcaac gaatcatttg taccaggaga tttgatgctg attaccgatc
    27781 atatcaattt tactggtgaa aatcctttga tcggtccgaa tgaagatgca atgggcccta
    27841 gatttccaga tatgagtcaa gcttatacgc ctgactatca agcaacagca aaagcagcag
    27901 cagaaaaact aggtttggat cttaaagaag gcgtatatat gggctattct ggaccaactt
    27961 atgaaacgcc tgcggagatc cgaatgtctc ggacaatagg agccgatgca gtagggatgt
    28021 cgactgttcc agaagtcatc gtagcagctc acagcggatt aaaagtgtta ggaatctctt
    28081 gtattaccaa cttagcagca ggtatgcaag cgaatctgag ccatgaagaa gttgtagaaa
    28141 caactcagag agtcaaacaa tcttttaaag cattggttaa agaaacattg acattattgt
    28201 aataggagga acaataaaat gagtgtgcat attgaagcga aagaaggcga gatcgccgat
    28261 aagattttac ttccgggaga tcctttgcga gctaaatata ttgcggagac ttttttggaa
    28321 gatccagttt gttacaatca ggtacgcggt atgttaggct ttaccggcaa atataaagga
    28381 gaacgtattt ctgttcaagg aacaggaatg ggaatgccat cagccaccat ctatgcccat
    28441 gaattgatcc agtcgtatgg tgtaaagaaa ttgattcgag taggtacatg cggtgcattg
    28501 tcaaaagacg tccatgttcg tgatttagta ctagcacaag gtgcagcgac cagttcttcg
    28561 atgatcgaaa aaaatttcca agcctttcat tttcctccaa tcagtgattt caatctatta
    28621 ctgaaagcat atgagatagc taaagaaaag ggctatacgg ttcatgtagg aaatgttctt
    28681 tcggaagatt ctttctacaa agatgatttg acagagacgt tccaattagc tgaacttggc
    28741 gttttaggag tggaaatgga agcagcggct ctttattact taggcgcgaa ataccatgtg
    28801 cagacattaa gtttgatgac cgttagcgac catttgatca ccggtgaaga aacaacagct
    28861 gcagaacgtc aatctacgtt caatgaaatg atcgaagtag gattggaaac agcgatagca
    28921 gaataagaag gaaatgaaaa agctgccctg cattaagtaa taagaacatc caagcaaaaa
    28981 tagctcctca tgtttttgac ttggctggac ttattattgc aaatgtaagc ttttgaacac
    29041 cgtttaccaa gaggttgtga atctgccgtt taactctgag tcacaaggaa caatttcaaa
    29101 aaacagcttt tcatgtttga tgaatattgt gacttgtgac cgagaagttg gcaggagaaa
    29161 accgtttata cgaaaaaagg gactgtgaca aatcgtgtca cagtcccttt tttatagtgt
    29221 tttttatagg tagtattttt tagcaactgt taagtcatca tttaattcat aaacaagtgg
    29281 ttgacctgtt ggaatttcaa gatccatgat gtcttcgtca gagatacctt cgatatgttt
    29341 agctagggcg cgtaatgagt taccatgtgc agctactaaa acagttttgt tgtctagtaa
    29401 tgctggagca atttcatctt gccagaatgg caaagcgcgt tctaatgtca ctttcaagtt
    29461 ttctccacca ggaatatcgc gtttgtctaa cattgcgtaa cgacggtcat ttgctgcaga
    29521 accttcgtca gtttcttcca ttaatggagg aagagtatcg taagaacgac gccagatgtg
    29581 cacttgttcg tcgccgtatt tttcagctgt ttcttttttg ttcaaacctt gtaatttacc
    29641 atagtgacgt tcgtttaagc gccatgattt gatttgtggt acccaaagtt gatctgaata
    29701 ttctaagatc aagttacatg ttttgattgc acgagttaaa acagaagtgt aagcaacatc
    29761 aaattcgatt ccagcttctt tgattttgcg tccgccttct tttgcttctt cgattccttc
    29821 tggtgctaga tttacatcag cccaaccagt aaattggttc aatgcattcc attcactaag
    29881 accgtgacga gaaaaaacta attttggcat gtttagctct ccttttaaga taggtttata
    29941 tttctgaaat tttcagttct gttttattgt acaatttttt ggaagagatt tccatcgaaa
    30001 aattagataa gattctatga gaaaacaagt agagaatata aaaaaacgaa cagtgcccac
    30061 gcaactgttc gtttttaggt attagatgca taattgctcg atttcttttt caggtaaatt
    30121 gatatattta taacatgttc caactgaaac accgcatttt gaagaaataa attggatcgt
    30181 ttcttttttt tcataataga gacgtcggat ttttttgatc gtttttgcat cgatttttgg
    30241 tcttccgccg atttttcctt ttttacgagc ttcatctaat ccaactaatg tacgttcttt
    30301 gatcaatgcg cactccatgt ttgctaggtt ttccattaat tggaaataga tttttcccat
    30361 ttcgcgcgta tcgattcctt catcaagact aatcaagtgc agcccgcgat ctttgaataa
    30421 ttgcgtgagt tctgttaatt gccgcgtaga tttgcctaat cgatgaagtt ggcaaatgac
    30481 tagtgcatcg ccttgttgca tgtcagcgat cactgactcc aaatgaacag cagtagaatc
    30541 attttgttcc acttcatatg tctcatggaa aatttcttga caaccaaagt cagataattg
    30601 atcaacctga gttgttaaat caacgtcatt tatggttgtg cgtgcataac caataatttt
    30661 catggtgatt cctttctagc agtgattttc taactggata taacagtaaa aatgtagcat
    30721 aaagtacgtc aaaaagatag ttttttcgca aattttccgc tgtctttttt tattggatga
    30781 tcaagcagtt tttctttatt tacgacggat tgcgtcaaga atctcaacgc ctttttctga
    30841 gccgacaact actttagaaa ccatgtttag gaaaagccca tgttctacta ccccaacaag
    30901 ttcatctaaa taggtagcta agctttctgg ctgttcgatg acttctagat gcagatcaat
    30961 gatgtaatga ccaccatctg tggttaagac ttctccgttt tcgtctgtac gcaagacggg
    31021 ttggaatctt ttttcttcaa aaatatgaag tagctgttgg gagccataag ggatcacttc
    31081 aactgggagt gggaacttcc caagtttatc gactaattta gaagaatcaa cgatccaaat
    31141 cgtttctttt gagtacgtag cgactatttt ctcgaaaagc aaagcagcgc cgccgccttt
    31201 gatcccttga aaatcagcac tgatttcatc agcaccgtct attgtcaaat cgactacggg
    31261 gacttcatca atactcttta acggtattcc taatttttcc gcttgttctt ttgtcgcatt
    31321 tgaagtagta actcctgtga tacgtaaacc ttcttcacgc atacggcgac caatctcttc
    31381 taccatataa taggcggttg aaccggtccc gagacctacg atcatcccgt cctttacgaa
    31441 tttcgcggct tcaatgccag ctaattgttt taaattcatc tttgcgtcca ccttttttta
    31501 gtttgagcat cgtgctttcc tcactatttt agcgtgattt acggtaaaaa gataccattc
    31561 ttttttattc caataaagct ttttcaaaaa aaattgcatc tcttgttgac atgttttttc
    31621 attcgtgata ttctaacaaa ggtgctttat ctacaggttt ctgtgaagaa acgtgctgac
    31681 tgtttatcaa agcatatttg ataaggatgc agacattgca tttatttttt atcacaaaaa
    31741 aagaaccacc tggatgtgtg gaactagacg caaatcgtgg gaaggaggaa acaaggaatg
    31801 cctacaatta accaattagt acgtaaacct cgtaaatcaa aggtggaaaa atctaattca
    31861 cctgctttga acaaaggata taacagcttt aagaaaactc aaactaacgt aaactcacct
    31921 caaaaacgtg gggtagcaac tcgtgtgggt acaatgacac ctaaaaaacc gaactcagct
    31981 ttacgtaaat atgcacgtgt tcgtttgtca aacttaattg aagttactgc ttatatccca
    32041 gggattggtc ataacttaca agaacacagt gttgtattat tacgcggtgg tcgtgtaaaa
    32101 gacttaccag gggtacgtta ccatatcgtt cgcggtgctt tagatactgc tggtgttaac
    32161 gaccgtaaac aaagccgttc taaatacgga actaaaaaac ctaaagctta attttaatta
    32221 ttaaacaaat gaaacttgct tctactatat agaagtaatt actatgaaca atatttcgga
    32281 aggaggagtt acggatgcca cgtaaaggtc ctgttgcaaa acgtgatgtt ttaccagatc
    32341 ctatttataa ctcaaaatta gtaactcgct tgatcaaccg tgtaatggtt gacggaaaac
    32401 gcgggattgc agctaacatt atctataatg cctttgactt gatcaaagaa tctacaggta
    32461 acgatccatt agaagtgttt gaacaagcaa tgaaaaacgt tcaacctgtt cttgaagtta
    32521 aagcacgtcg tgttgggggt tcaaactacc aagtaccagt tgaagttcgc cctgaacgcc
    32581 gcgttactct aggtcttcgt tgggtagtaa actacgctcg cttgcgcggt gaacacacaa
    32641 tggaagaacg cttagcaaaa gaaatcatgg atgctgcaaa caacacaggt gcttctgtta
    32701 aaaaacgtga agacactcat aaaatggctg atgccaaccg tgcgtttgct cactatcgct
    32761 ggtaagattc tctctgtttg gtcggctttt ataagtcgac ctcagatagt gtccttattt
    32821 ttagcaacta gatagtaaag agaggagaaa caaggagaat gaccagagaa ttttctttac
    32881 aaaacactcg taatatcggt atcatggccc acatcgatgc tggtaaaacg actgctactg
    32941 aacgtatctt gtactatact ggtaaaatcc ataaaattgg tgaaactcat gaaggagctt
    33001 cacaaatgga ctggatggaa caagaacaag aacgtggtat tacgatcact tctgctgcta
    33061 ctacagcaca atggaagggt caccgaatca acatcatcga cacaccagga cacgtagact
    33121 tcacagttga agttgaacgt tcattgcgtg tacttgatgg tgctgtaact gttcttgatg
    33181 cacaatcagg tgttgaacct caaactgaaa cagtttggcg tcaagcaaca acttatggtg
    33241 tacctcgtat cgtatttgca aacaaaatgg acaagatcgg tgctgacttc ttatattcag
    33301 tatcaacttt acatgaccgt ttgcaagcaa acgctcaccc aatccaatta ccaattggtg
    33361 cggaagacga cttcacagga attatcgacc ttgtaacaat gaaggctgaa atgtatacaa
    33421 acgatcttgg aacagagatc gaagaaacag aaattcctga agaataccgc gaattagctg
    33481 aagaatggcg cgaaaaattg gttgaagctg tagctgaaac tgatgaagaa cttactttga
    33541 aatacttaga aggtgaagaa atcactgaag ctgagttaaa agaaggtatc cgccgcgcaa
    33601 cagttaacgt agaattctac ccagtactat gtggttcagc atttaagaac aaaggggttc
    33661 aattactatt ggacgctgtt cttgattact tgccatcacc gttagatatc ccagctatca
    33721 aagggattga ccctaaaact gatgaagaag tagaacgtcc tgcagacgac agcgctcctt
    33781 tctcagcttt agcatttaaa gttatgactg acccattcgt aggtcgttta actttcttcc
    33841 gtgtgtactc tggtgtttta caatcaggtt catacgtaca aaacgcaaca aaaggcaaac
    33901 gtgaacgtgt tggtcgtatc ctacagatgc atgccaactc tcgttcagaa atctctgaag
    33961 tttacgctgg agatatcgct gctgctgttg gtttgaaaga cacaactact ggggacacac
    34021 tatgtgatga aaagagtcta gttattcttg aatccatgga attcccagaa ccagttatcc
    34081 aagtcgctat tgaacctaaa tcaaaagctg accaagataa aatgggtgtg gctttacaaa
    34141 aactatctga agaagatcct actttccgtg ctgaaacaaa cgtggaaaca ggcgaaacaa
    34201 tcatcgctgg tatgggtgaa cttcacttag atatcatcgt tgaccgtatg cgtcgtgaat
    34261 tcaaagttga agctaacgta ggtgcgcctc aagtatctta ccgtgaaact ttccgtgctg
    34321 gcactcaagc cgaaggtaag tttgtacgtc agtctggtgg taaaggtcaa tacggtcacg
    34381 tatggattga atttacacca aatgaagaag gtgctggttt cgaatttgaa aatgcgatcg
    34441 tcggtggggt tgttcctcgt gaatacattc cagctgttga aactggtttg aaagatgcta
    34501 tggaaaatgg tgtcttagct ggctatccat tagtagatat caaagctaaa ctttacgatg
    34561 gttcatacca tgatgtcgat tcaaatgaaa ctgccttccg tgttgctgct tctatggcat
    34621 tacgtgcagc agctaagaaa gctaatccag taatccttga accaatcatg gctgtagaag
    34681 ttgttatccc tgaagattat ttaggtgatg ttatgggtca cgtaactgct cgtcgtggac
    34741 gtgttgaagg tatggaagct cgcgctggcg gccaacaagt tgttcgtgcg atggttccac
    34801 ttgctgaaat gtttggttat gctacaactc ttcgttctgc tactcaaggt cgtggtacat
    34861 ttacaatgac atttgaccac tacgaagatg taccaaaatc agtacaagaa gaaatcatta
    34921 agaaaaacgg cggaaaagct gaataatttc ttgaaaccgg atagtcctgc aaagagatta
    34981 tccaaagaga ctatattttt aatatagttt cgtgtaaaat aattatcgat gatatgggca
    35041 tgtggtgtac cttgtgtaca tcatatacct atcagacaaa taaaaaatat attgtatttt
    35101 taggaggaca ttttaaaatg gcaaaagaaa aatttgaccg ttctaaacca cacgttaaca
    35161 ttggtactat cggacacgtt gaccatggta aaactacatt aacagctgca atcacaactg
    35221 tactatctaa gaaaaacggc ggccaagcta tggcttacga tcaaatcgat ggtgctccag
    35281 aagaacgcga acgcggaatc acaatctcaa cagctcacgt tgagtatgaa acagatacac
    35341 gccactacgc tcacgttgac tgcccaggac acgcggatta cgttaaaaac atgatcactg
    35401 gtgctgctca aatggacgga gctatcttgg tagtttctgc tgctgacggc ccaatgcctc
    35461 aaactcgtga acacatccta ttgtctcgtc aagttggtgt tccttacatc gttgtattct
    35521 tgaacaaagt agacatggtt gatgacgaag aattactaga attagttgaa atggaagttc
    35581 gtgacctatt aacagaatac gaattccctg gtgacgatgt tcctgtagtt gctggatcag
    35641 ctttgaaagc tctagaaggc gacgcttcat acgaagaaaa aattcttgaa ttaatggctg
    35701 cagttgacga atacatccca actccagaac gtgacaacga caaaccattc atgatgccag
    35761 ttgaagacgt gttctcaatt actggacgtg gtactgttgc tacaggtcgt gttgaacgtg
    35821 gacaagttcg cgttggtgac gaagttgaag ttgttggtat tgctgaagaa acttcaaaaa
    35881 caacagttac tggtgttgaa atgttccgta aattgttaga ctacgctgaa gctggagaca
    35941 acattggtgc tttactacgt ggtgttgcac gtgaagacat ccaacgtgga caagttttag
    36001 ctaaaccagg tacaatcaca cctcatacaa aattctctgc agaagtatac gtgttgacaa
    36061 aagaagaagg tggacgtcat actccattct tcactaacta ccgtccacaa ttctacttcc
    36121 gtacaactga cgtaacaggt gttgttgaat taccagaagg aactgaaatg gttatgcctg
    36181 gcgacaacgt aacaatggaa gttgaattaa tccacccaat cgctatcgaa aacggtacta
    36241 aattctcaat ccgtgaaggc ggacgtacag ttggtgccgg tgtcgttaca gaaatcaaag
    36301 cttaattcac ttaagtttta aagtaaagca acatttattc ggacgtaatc gctcggaacg
    36361 aaggtcttag gatctttgtt ccgagctttt ttatttattg caggatcagg aaaaaagaca
    36421 ggagtattat aaatagaaga aacacgaaat aaaagagtca gtattttcta aatgaaactt
    36481 tatgaaaaca aaatgttttt tacaagaaaa agccttgctt tgtgagttta tatatattat
    36541 aatgacaaag gtgcgtttac tttagtgaat tgcacagacg taatcagaat gatttcgtcg
    36601 gctaagaaac gagaggttgc gacacgcccg gacgctttgc cacgaacgag cgtgacggaa
    36661 attttcgtgg agctatgtct acttttcaaa ataggcgaag gagggaacaa gatggcaaaa
    36721 caaaaaattc gtatccgttt aaaagcgtat gaacaccgta ttttagatca atcagcggat
    36781 aaaatcgtgg aaactgcaaa aagaactgga gctagcgtgt ctggtccaat tccattacca
    36841 actgaacgca gtctttacac aatcatccgt gcgactcata aatacaaaga ttcacgcgaa
    36901 caattcgaaa tgcgtacaca caaacgtctt atcgacattg tgaacccaac accaaaaact
    36961 gttgatgctt tgatgaagct agacttacca tctggtgtaa acatcgaaat caaattataa
    37021 aactaaaaca aaaataatgg aggtgtactc atgaccaaag gaatcttagg gaaaaaagtg
    37081 ggaatgacac aaatcttcac tgaatctggt gaattaattc cagtaactgt tgtagaagct
    37141 actccaaacg tagtactaca agtgaaaaca atggaaacgg atggctacga agctattcaa
    37201 gttggttacc aagatatgcg tgaagtttta tcaaacaaac ctgcgaaagg tcatgttgca
    37261 aaagcaaaca cggctcctaa gcgcttcatt cgtgaattca aaaatgttga gctaggagaa
    37321 tatgaagtag gaaaagaaat tacagtagat gttttccaag caggagacat tattgatgta
    37381 acaggtacta cgaaaggtaa aggattccaa ggggttatca aacgccacgg acaaagccgc
    37441 ggaccaatgg ctcacggttc tcgttaccat cgtcgtcctg ggtcaatggg tccagttgca
    37501 cctaaccgtg tatttaaaaa caaacgtctt gctggccgta tgggtggcaa ccgcgtaact
    37561 atccaaaact tggaagttgt acgtgtcgat gcagaaagaa atgttatctt gatcaaaggt
    37621 aatgttcctg gagctaaaaa atctttaatt actatcaaat cagctgtgaa agctaaataa
    37681 ttgtaggaag aaaggaggaa ctaaggaatg ccgaatgtag cattattcaa acaagatgga
    37741 agccaaaacg gtgaaatcac acttaacgaa gaaatcttcg gaattgaacc taatgaaagt
    37801 gttgtttacg atgcaatcgt aatgcaacgt gcgtcattaa gacaaggaac acacgcggtt
    37861 aaaaaccgta gcgctgttcg cggcggcgga cgtaaaccat ggcgtcaaaa aggaacaggt
    37921 cgtgcacgtc aaggatctat ccgctcacca caatggcgtg gaggtggcgt agtctttgga
    37981 ccaacaccac gttcttacag ctacaaactt cctaaaaaag ttcgtcgttt agcaatgaaa
    38041 tctgtattgt cagaaaaagt tgctgaaaac aacttggtag caatcgaagg cttgaacttc
    38101 gacgcaccaa aaacaaaaga attcaaacaa gttcttgcta acttatctat cgactctaaa
    38161 gtattggttg ttctagaaaa aggtaacgac tttgctgctt tatcagcacg taacttacca
    38221 aacgtttctg tagtaacttc tgataatgtg agtgtgctag acgttgtatc aaatacaaaa
    38281 gtgttagcaa cacaaactgc tcttactcaa attgaggagg tgcttgcata atgaacttac
    38341 tagacgtaat caaacgccca gtgatcactg aaaaatcaat gcttgccatg gatgacaaga
    38401 aatacacttt tgaagtggac actcgcgcaa acaaaacttt agtaaaacaa gcggtagaag
    38461 ctgcttttga cgttaaagtc aaaaacgtga acatcgtgaa cgtacgtgcg aaattcaaac
    38521 gtatgggcaa atatgcagga tatacaaaaa aacgtcgcaa agcgatcgtt acattgactg
    38581 aagattcaaa agaaatccaa ttattcgaag ctgctgaata agcactaata gataactcat
    38641 taaattagga gggaaaaacg tggcgattaa aaagtacaaa cctacctcaa atggtcgtcg
    38701 taacatgaca ggttctgatt ttgctgaaat cacaacatca acacctgaaa aatcattgtt
    38761 acaaccattg aaaaaccatg ctggacgtaa caacaacggc cgcatcactg tacgtcatca
    38821 aggtggcgga cacaaacgcc aatatcgcgt gatcgacttc aaacgtaaca aagataacgt
    38881 tgttgcaact gttcaaacga tcgaatatga tccaaaccgt tcagctaaca tcgcgttagt
    38941 acactatgaa gatggagtga aagcttacat cttagcacct aaaggactag aagtgggtat
    39001 gaaacttgtt tcaggtccag aagcagatat caaagtcgga aatgctcttc cattagaaaa
    39061 cattccagtt ggtactgtta tccacaacat tgaaatgaaa cctggtaaag gtggacaatt
    39121 gatccgttct gctggtacaa gtgctcaagt acttggtaaa gaaggcaaat acgtattgat
    39181 ccgcttgaac tctggtgaag ttcgtatgat cttagctgca tgccgtgcta caatcggttc
    39241 tgtaggtaac gaacaacacg aattaatcaa catcggtaaa gctggacgct ctcgttggat
    39301 gcgtaaacgc ccaactgtac gtggtagcgt aatgaaccct aatgatcacc cacacggtgg
    39361 tggtgaaggt aaagctccta tcggacgtaa agcaccagtt tctccttggg gtcaaccagc
    39421 tcttggttac aaaactcgta acaaaaaagc taaatcagac aaacttattg ttcgtcgtcg
    39481 caaaaataaa taattacttt gaccatgtgt cgcacatttt gagaggaggt tcaccatggg
    39541 tcgtagttta aagaaaggac cttttgtcga tgagcattta atgaaaaaag ttgaagctca
    39601 agcaggggtt gaaaagaaaa aagttatcaa aacttggtca cgccgttcaa cagttttccc
    39661 aagttttgtt ggatatacca tcgcagttta tgatggacgt aaacatgtac cagtttacat
    39721 ccaagaagac atggtaggac acaaattagg tgaatttgca cctacaagaa cttatcgtgg
    39781 acatgctgct gacgataaga aaacgaaacg ctaatttgag gggaggataa atccaaatgg
    39841 cagaacaaat cacatcagct aaagcaactg caaaaacagt tcgcacttca cctcgaaaag
    39901 cccgtttagt aattgacctt atcagaggta aaagtgtcgc agatgcaatt tcaatcttga
    39961 aattcacacc aaacaaatct gctggaatca ttgaaaaagt attgatgtca gcagttgcga
    40021 atgcagaaaa taactttgac ttagatgttg aaaacttggt agtaagcgaa gcttttgtta
    40081 acgaaggacc tacaatgaaa cgtttccgtc ctcgtgcaaa aggatcagct tctccaatca
    40141 acaaacgtac aagtcatatc acggtagtcg tatcagaaaa ataaggaggg ataatcagtg
    40201 ggtcaaaaag tacatccaat tggaatgcgt gtaggcatca tccgcgactg ggatgctaaa
    40261 tggtatgctg aaaaagagta tgctgagttc ttacacgaag atttaagaat ccgtaaattt
    40321 atttcaacta aacttgctga tgctgctgta tcaacagttg aaatcgaacg cgcaacaggt
    40381 cgcgtaaaca tttcaattca cacagctaaa ccaggtatgg ttattggtaa aggcggttct
    40441 gaagtcgaaa gcctaagaaa agaattaaac aaattaactg gtaaaagaat ccatatcaat
    40501 atcgtggaaa tcaaaaaacc agatctagat gcaaaattag taggtgaagg aatcgcacgt
    40561 caattagaaa accgtgttgc tttccgtcgc gctcaaaaac aagccatcca acgctcaatg
    40621 cgtgctggcg ctcaagggat caaaactcaa gtatctggtc gtctaaacgg agcagatatc
    40681 gctcgttcag aaggatactc agaaggaact gttcctcttc atactttacg tgcggacatt
    40741 gattacgcat gggaagaagc agatacaaca tacggaaaac taggagttaa agtgtggatc
    40801 tatcgtggag aaattcttcc aactaaaaaa aacactgaga aaggagggaa ataatcatgt
    40861 tagtacctaa acgtgtaaaa caccgtcgtg aattccgcgg aaaaatgcgc ggggaagcta
    40921 aaggcggaaa agaagtagca ttcggtgaat acggtttgca agctgttgat tcacattgga
    40981 tcacaaaccg ccaaatcgaa gctgctcgta tcgcaatgac tcgttacatg aaacgtggtg
    41041 ggaaagtatg gattaaaatt ttccctcaca aatcttatac tgccaaagca attggggtac
    41101 gtatgggttc tggtaaaggg gcacctgaag gatgggttgc accagtaaaa cgtggtaaaa
    41161 tcatgtttga aatcgcaggc gttcctgaag aagtagctcg tgaagcgtta cgtctagctt
    41221 ctcacaaatt accaatgaaa actaagatcg taaaacgtga ggaaatgggt ggtgaatcga
    41281 atgaaggtta aagaaatcag agaattaacc actgccgaaa tgcttgatca agaaaaacaa
    41341 ttaaaagaag aattgtttaa tcttagattc caattagcaa caggtcaatt agaaaacact
    41401 gcacgtatta aagaagtacg taaatcgatt gcacgcatca aaactgtgtt gcgtgaacaa
    41461 gctaagtaat tatggaagga ggccattaag cgtatgactg aaggaagaaa tcaacgcaaa
    41521 gtttatcaag gtcgcgtggt ttcagacaaa atggataaaa caattaccgt tgctgtcgaa
    41581 acaaagaaaa accaccctat ctatggcaaa cgcatgaact attctaagaa atacaaagtg
    41641 catgatgaaa acaacacagc aaaagttggc gacatcgtga gaattatgga aactcgtcca
    41701 ttatcagcta caaaacgttt ccgtttgttg gaagtagtcg aagaagcagt tattatctaa
    41761 taacacgaga ttttcctata tagattgaat gcaaaatctg aaaggaggat acacaacgtg
    41821 atccaagcag aaagtcgttt aaaagtcgct gataactcag gcgcacgtga aattttgacg
    41881 atcaaagtcc ttggtggatc tggtcgtaaa actgctaaca tcggtgatgt gatcgttgct
    41941 acggttaaac aagcaacgcc aggtggggtt gtcaaaaaag gtgacgtcgt taaagccgtt
    42001 atcgttcgaa caaaatcagg agctcgtcgt acagacggtt catatattaa atttgatgaa
    42061 aatgctgcag tgattattcg tgatgataaa agccctcgtg gaacacgtat ctttgggcca
    42121 gttgcacgtg aactacgtga aaacaacttc atgaagatcg tttcactagc accagaagtt
    42181 ttataatctt gagacactta tcaaaggagg tgcgaaacag ttatgtttgt taaaaaaggc
    42241 gataaagtta aagttatcac tggaaaagac aaaaacaaag aaggcgttgt actagcagcg
    42301 ttcccgaaaa aagataaagt tattgtcgaa ggtgtaaatg tcgttaaaaa acaccaaaaa
    42361 ccttctcaag ctgctcctca aggtggaatt gttgagatgg aagcgccaat tcatgtttct
    42421 aacgtgatgg tggttgactc tactggtgta gctggccgag ttggctacaa agaagtagac
    42481 ggcaaaaaag tccgtgtttc taaaaaaacc ggtgaagttt tagataaata aaagtttaag
    42541 gaaggagggt cttactgaat gaaccgccta aaagaaaaat atcttaaaga agtaactcca
    42601 tcattgatgg aaaaatttaa ctatagctct gttatgcaaa cacctaaagt tgaaaaaatc
    42661 gtcatcaaca tgggtgttgg tgatgcggta tcaaacgcta aaaacttaga taaagcagtt
    42721 gaagaattaa ctttgatcac tggtcaaaaa ccaatgatta caaaagctaa aaaatcaatc
    42781 gctggattcc gtttacgtga aggaatgcca attggtgcaa aagttacatt gcgcggagaa
    42841 agaatgtacg aattcttaga caaattggtt tctgtttcac taccacgtgt acgtgacttc
    42901 catggtgtaa gtaaaaaagc ctttgacggt cgtggaaact atactttagg tattaaagaa
    42961 caattgatct tcccagaagt agattatgat ttagtagata aagtacgtgg tatggacatc
    43021 gttattgtaa caacagcaaa ctctgatgaa gaatctcgtg agttgttgac acaattaggc
    43081 atgccattcc aaaaataatt aaaggaggcg aactacgtgg ctaaaaaatc aatgattgct
    43141 aaaaacaaac gtcctgcaaa attttcaaca caagcttata ctcgttgcga acgttgcgga
    43201 cgtccacatt cagtttatcg taagttccat ctttgccgta tttgcttccg cgaacttgcc
    43261 tataaaggtc aaattcccgg cgtgaagaaa gctagctggt aaaaagtaaa ttcgcataag
    43321 aaggaggtaa aagatcaatg gtcatgacag atccaattgc agattttcta actcgtatcc
    43381 gtaacgcaaa catggttaaa catgagtctt tagaagtacc tgcatcaaaa attaaacgtg
    43441 acatcgctga aatcttgaaa cgtgaaggtt tcgttcgtga tgttgaatac atcgaagatg
    43501 acaaacaagg cgtgatccgt gttttcctaa aatttggtaa aaacggcgaa cgtgttatca
    43561 caaacttaaa acgtatctct aaaccaggtt tgcgtgctta tgtcaaagct gacgaagtac
    43621 caaaagtatt gaacggttta ggtatcgcaa tcatttcgac ttctgaaggt gttgtcactg
    43681 ataaagaagc tcgtgaaaaa aatatcggcg gcgaagttat cgcttacgta tggtaatcaa
    43741 gtagaatcta caaggaggtg tctttaagtg agccgtatcg gaaataaagt cgttgttatc
    43801 cctgaaggag taacagttac tcaagacgga aacaacgtta cagttaaagg accaaaaggt
    43861 gaattgactc gtactttttc agcagatatc aaaatgaata tcgaaggaaa cgaagtaact
    43921 ttcactcgtc caaacgacag taaagaaatg aaaacaattc atggaacaac tcgtgcgaac
    43981 ttcaacaaca tggttgtagg tgtcagcgaa ggtttccaaa aagcgttaga actaatcggg
    44041 gttgggtacc gtgcacaatt acaaggatca aaacttgtat tgaacgttgg ttactcacat
    44101 ccagtagaaa tcacaccacc agctggagta actgttgaag taccagctaa cacacaagta
    44161 attgttaaag gtgcgaacaa agaagaagtt ggcgaattag ctgcaaacat tcgtggaact
    44221 cgtcctccag aaccttataa aggtaaaggt attcgttatg ttggcgaata tgtacgccgt
    44281 aaagaaggta aaactggtaa ataatcgttt agaaaaattg catagaagcc agcagcagga
    44341 aaaaggcatt tggctgaagt ttaccttcaa aaaaatacat ttttacttgt gccgcagctt
    44401 ctatttttcg aaacgtattt actgcttgaa atagtagtac aataaaaata aagaggtgac
    44461 aattgtgatt acaaaaccag ataagaacaa aacacgtcaa aagagacatc gtcgtgtacg
    44521 taacaatatc tctggtactg ctgagcgccc acgcttgaac gtttttcgtt ctaacaaaaa
    44581 catctacgct caaattattg atgacgtagc gggtgtaacg ctagcaagtg cctctacctt
    44641 ggataaagaa atttcaggtg gaacaaaaac agaagcagca caagctgtcg gtaaactagt
    44701 tgctgaacgt gcagctgaaa aaggcattaa agtagtagtc tttgaccgtg gtggatacct
    44761 ttaccatggc cgtgtgcaag ctttagctga agctgctcgc gaaaatggac tagaatttta
    44821 gaagaaggag gaataccatt catggtttat atcgatccaa aacatttgga attagaagac
    44881 cgcgttgttg caattaaccg tgtaacaaaa gttgttaaag gtggacgtcg tctacgtttt
    44941 gctgctttag ttgttgtcgg tgacaaaaac ggacacgtag gatttggtac aggtaaagct
    45001 caagaagttc ctgaagcaat ccgtaaagca attgaagatg cgaagaaaaa cttagttgaa
    45061 gtacctatgg ttggttcaac aatcccacac gaagtaatcg gtgcttttag tggtggtaga
    45121 atcctaatga aacctgctgt agaaggttct ggggttgctg ctggtggacc agttcgtgcc
    45181 gtattagaat tagctggggt agcagatatc acttctaaat ctttaggctc aaatacacca
    45241 atcaacgttg ttcgcgcaac agttgaaggt ttgaaacaat tgaaacgtgc agaagaagtt
    45301 gcagaacttc gcggcaaatc agttgaagaa ttaatcggat aaggaggaca acaataatgg
    45361 ctgaattaaa agttacttta aaacgcagta ttatcggacg tcctcaaaac caacgcgata
    45421 cagttaaagc gttaggtctt acaaagatta acagttcagt ggttaagcct tctaatgaag
    45481 caattaaagg catgatcaat actgtttctc acctagtgga cgttgaagaa atttaattta
    45541 gaaaaatcat aactagaaca ttaaggaggt gccaaaccta tgaaacttca tgaattaaaa
    45601 cctgctgaag gttcacgcca agtacgtaac cgtgttggtc gtggtacttc atctggtaac
    45661 ggaaaaactg ccggacgcgg acaaaaagga caaaaagctc gttcaggcgg cggtgtgcgt
    45721 ttaggatttg aagggggaca aacaccattg ttccgtcgtt taccaaaacg aggattcaca
    45781 aacgtgaacc gtaaagacta tgcagtcatt aatcttgatg tattgaaccg ctttgaagac
    45841 ggagctgaag taactcctgt tgctttagtt gaagctggaa tcgtgaaaaa cgaaaaagct
    45901 ggaatcaaag tattagctaa tggggaacta aacaagaaat taactgtgaa agcagcaaaa
    45961 ttctctaaag cagctcaaga agcaattgaa gcggctggtg gatcaatcga ggtgatctaa
    46021 tgtttagact attaaaagat gcttttaaag taaaagacat tagatcgaaa atcctattta
    46081 ctgtatttgt gttgtttgta tttcgcctag gtgcacatat tactgtacca ggcgtaaatg
    46141 ctgcttcatt gcaaaacctt tcttctttgc catttttcaa catgttgaac ttggtaagtg
    46201 gtagtgcgat gcaaaacttt tccattttct caatgggggt ttcaccatac attactgctt
    46261 ctatcgtcat tcagcttctt caaatggaca tcgtgcctcg atttgttgaa tggtcgaaac
    46321 aaggggaagt tggtcgtaaa aaactgaatc aagcgactcg atatctgaca ttagtattgg
    46381 catttatcca atctgtcggt atcactgctg gattcaatca atacgctcaa ttaggtttcg
    46441 tgaataatcc taacgtagca acttacgtaa cgatcggttt gattctaaca gccggtacga
    46501 tgtttgttac ttggctagga gaacagatca cagaaaaagg tatcggaaat ggcgtatcaa
    46561 tgatcatttt tgcaggtatc atttctagat tgcctgaaag tatcagagaa atttatgaag
    46621 attactttat aaatattgat ccttcagaca tatggaaatc tgctattttt gtggccattt
    46681 tactagtagc ggtcttgttg atcgtgacat tcgtaacatt tttccaacaa gctgaacgca
    46741 agatcccgat ccaatatacg aaacgcgtag ctggcgcgcc tacaagcagc tatctgccgt
    46801 tgaaagtaaa tgcagctgga gttattccag ttatctttgc tagttcgttt attgctacgc
    46861 caaatgctgt tttacaagca ttgagcggta gattcggttc tgaaaactgg tacaaagtga
    46921 tgatggagat ctttaactat aatacagcac ctggtgctac gatttatacg attctgatcg
    46981 ttgccttcac tttcttctac gcctttgttc aagtaaaccc tgagaaatta gcggaaaact
    47041 tgcaaaagca aggaagttat ataccaagcg tacgccctgg taaggggact gaagagtatg
    47101 tctcaagatt actgatgcgt ttaagtacag ttggtgcagt cttccttggt ttagtggcat
    47161 tgcttccaat cattgctcaa atggtttggg atcttccaca atcaatcgga ttaggcggta
    47221 caagcttact gatcgttatc ggtgtagctt tagaaactgc gaaacagtta gaaggcttaa
    47281 tgctgaaacg taaatatgtt ggattcattg agtaagaaag cgtgttgagg tttcccctca
    47341 acacacgttc tcacacattt attaggaggg aaaacaatga acctcatttt aatggggctg
    47401 ccaggcgcag gtaaaggaac acaagctgaa aaaatcattg atgcttatgg tattccgcac
    47461 atctcaacag gagatatgtt ccgcgcagcg atgcaaaacg aaacagctct tggcctggaa
    47521 gcaaagtctt atatggataa aggtgcatta gttcctgatg aagtaacaaa tggaatcgtg
    47581 aaagagcgac tagctgaacc agatacagag aaaggcttct tattagatgg ctttccacgt
    47641 acgctagatc aagcaaaagc tctggacgca atgctaaaag atttgaataa aaaaattgat
    47701 gctgtcatcg atatccatgt tggtgaagaa atcttggtcg aacgtctagc cggccgattt
    47761 atctgcagca attgtggagc aacttaccat aaaattttca accctacaaa agttgaagac
    47821 acatgtgatc gttgcggtgg gcatgaattc tatcaaagag aagatgataa acctgagacg
    47881 gttaaaaatc gtctggctat caacatcaaa aatagtgaac caatcttggc ttattataaa
    47941 gaacaaggtt tgctaaacac tattgatggt gctcgtgaaa tcgatgctgt atttgcagat
    48001 gtacaaaaaa tcattgaaaa atagcacgat tgcccgtagc cgacttgcat tatgcagtta
    48061 ctcttgtcaa gttcaataaa ttatgataga atatgtcagt ccgacttcta gagaatcctc
    48121 tagatgatcc attttgagaa ttttgaggtg ggaactgcag tttccgccat tttatcgtgt
    48181 gaaaatgcga aacaagtaca aggaggtact gtgcgtggca aaagaagata tgattgaagt
    48241 cgaaggtaca gtcgtcgaaa ctttgccgaa tgcaatgttt aaagttgaac tagaaaacgg
    48301 acaccaagtt cttgctactg tttcaggtaa aattcgtatg cattacatcc gtatcttacc
    48361 tggtgacaaa gtaacggttg aattgtctcc atacgatctt aaccgtggcc gtattactta
    48421 tcgctttaaa taattgtact ccgtaaaact acaggaggta ttatcatgaa agtaagacca
    48481 tcagtaaaac caatgtgcga acattgtaaa gtaattcgtc gtaaaggacg cgttatggtg
    48541 atttgcccag caaatccaaa acataaacaa cgtcaaggat aattggaggt gctataaata
    48601 tggctcgtat tgcaggagta gatattcctc gtgataaacg tgtagtagtt tcacttactt
    48661 atatctatgg tattggtaac actactgcta aaaagatctt agctgatgct ggtgtatctg
    48721 aagatgttcg cgttcgtgaa ttaacgaatg aacaaacaga tgctatccgt gcagaagtag
    48781 ataaattaaa agttgaaggg gatcttcgtc gtgaagtgaa cttgaacatc aaacgtttga
    48841 tggaaatcgg ttcttaccgt ggaatccgtc atcgccgtgg attgcctgtt cgtggacaaa
    48901 acacgaaaaa caatgcacgt actcgtaaag gcccagctcg cgctatcgct ggcaagaaaa
    48961 aataattctt aagtgaagga ggttaaaact tcatggcagc aaaaaaagtg aatcgtaaac
    49021 gccgtgtgaa gaagaatata gaagcaggta ttgcacatat ccattctaca ttcaataaca
    49081 ctatcatcat gatcactgat actcacggaa atgcattagc gtggtcatca gctggttcat
    49141 taggtttcaa aggaagcaaa aaatcaactc cttttgccgc tcaaatggca gcagaaactg
    49201 ctacaaaagc agcaatggaa cacggattaa agactgttga agtaacagtt aaaggacctg
    49261 gttctggacg tgaagcagca attcgttcat tacaagctac aggtttagaa gtgactgcaa
    49321 ttcgtgacgt gactccagtt cctcataatg gtgcccgccc tccaaaacgc cgtcgtgttt
    49381 aatgagtgcc gctcattttg agttttatca gtaacgtcat tgaacaagac acttttcgtt
    49441 ttgaaagggg taaagataag aatgattgag ttcgaaaaac caagaatcgc aaaaattgat
    49501 gaagaaaaag attatggcaa gttcatcgtt gaacctcttg aaagagggta tgggactact
    49561 ttagggaatt ccctacgtcg tattttatta tcttctttgc caggtgctgc catcactaat
    49621 attcaaatcg atggtgtact gcatgaattc tctaccatca aaggtgttag agaagacgtc
    49681 actcaaatca tcttgaacat taaaggctta gcgctgaaaa tgtatggcca agaagaaaaa
    49741 acccttgaaa tcgatattac cggacctgct acagtaactg ctggcgatat tatcgttgat
    49801 agtgatgtag aaattcttaa caaagatatg tacatctgta gtgtctctga aggagctacg
    49861 ttccatgctc gcttaacagt gaaacctgga cgtggttatg ttcaagcaga tgaaaataaa
    49921 aaagaagata tgccaattgg cgttcttcca gtcgattcaa tctacacacc tgttcgtcgt
    49981 gtgaactatc aagttgagaa cacacgtgtt ggacaccgtg aagattttga taaattgacg
    50041 atggagattt ggacagatgg ttcaattgaa ccgttggaag caatgagttt gtctgcgaaa
    50101 atcatgactg aacatttgga tatttttgtg aacttaactg atgaagcaaa aaatgctgaa
    50161 atcatggtcg aaaaagaaga aacacaaaaa gaaaaaatgt tggaaatgac gatcgaagag
    50221 ttagacttgt ctgttcgctc ttacaattgt ttgaaacgtg caggaatcaa tactgtacaa
    50281 gaacttacaa ataaatctga gcctgaaatg atcaaagtcc gtaacttagg acgtaaatca
    50341 ttagaagaag ttaaagcaaa attgcacgac ttaggtttag gtttacgtaa agaagattaa
    50401 aaccttttac gaaggaggga aaccaacgtg agttatcgta aactaggacg cacatctagc
    50461 caacgtaaag cgatgttgcg tgatttaaca actgatttat taatcaacga acgtattgtg
    50521 actactgaag ctcgtgcgaa agaagttcgc tcaactgcag aaaaaatgat cactttaggt
    50581 aaacgcggag atttgcatgc acgtcgtcaa gctgcggctt tcgtacgtaa tgaagttgcc
    50641 agcgtgcgtg aagaaaatga ggacatcgtc attgaatcag ctttacaaaa actgttcaac
    50701 gatattgcac ctcgttacgc agaacgtcaa ggcggataca cacgcatttt gaagacagaa
    50761 ccaagacgcg gtgatggtgc accaatggtt gtcttagaac ttgtctaaaa tcacactaat
    50821 aacatcactt tatagatgat tcttttagag tgttatgatg ttggaagata tttttccgag
    50881 tctagctcag cgctgctcct caagcgaata tcgcttgggg agtaagcact catcataaag
    50941 tgttttttta taccttcaat tacctgtcat gacagatatg tttgaaggca taaaaaaaca
    51001 ttttaagcga gaaataattg aaaattttgt ctttttttga tagtgttagt aggaacttta
    51061 ggatgaagaa ggagtaatca tggagccaat tattgaatta gaaaagataa attataaata
    51121 ccagccagat gatcttcgcc ccgcattgaa agatgtctcc tttacaattg ataaaggtga
    51181 gtggatcgct attattggac acaatggttc aggaaaatcg acacttgcca aaacgatcaa
    51241 tggattgctt ctgccagaat cagggatcgt gaaagtggga aatcaaatat tggatgaaga
    51301 aaatatttgg acgatccgac agatggttgg aatggttttt caaaatccag acaatcagtt
    51361 tgttggttcg acagtagaag atgacgttgc ttttggtctt gaaaatcaag ggattcccag
    51421 agaagaaatg ctagtgcgtg tgaaagatgc acttgagaaa gttagaatgg ctgagtttgc
    51481 ttcacgcgag cctgctcgtt tatcaggagg gcaaaaacaa cgtgttgcca ttgccggcgt
    51541 ggttgcctta cgacctgata tcattatttt agatgaagca accagtatgc tagatccaga
    51601 aggtcgtgaa gaagtgatct ctacgatcaa gaagatcaaa gaagaaagtc aattgaccgt
    51661 tatatctatc actcatgata ttgatgaagc agctaatgcc aatcggatac tagtgatgag
    51721 acaaggcgaa cttgtccgtg aaggtacacc aaaagaaatt ttttctgctg gtcctgaact
    51781 gatcgactta ggtctagatc taccatttcc tgaaaaactg aagagcgcac tgaaagaacg
    51841 tggggttgat gtgcccagtg aatatatgac agaagaaagg atggtggatt ggttatggac
    51901 atccgttttg aacaagtaga cttcacttat cagccgaata caccatttga acaaagagcc
    51961 ttatttgaca tcaatatgac gatcaaagaa aacagttata ctgcattagt agggcatacc
    52021 ggaagcggga aatcaacctt acttcagcat ttgaatgcgc ttgttaagcc aacaagcggt
    52081 acagtccata ttggagaacg agatattcag ccggatacgg ataataagaa tttaaagccc
    52141 atccgaaaaa aagttggcat cgtctttcag tttccagaag cacagctatt cgaagaaacg
    52201 gtagcaaaag acattgcttt cggtcctaaa aactttggtg tcagcgaaga agaagcacta
    52261 gtcctagcaa aagaaacatt agaacaagtt gggctggatg aaagctattt ggaacgttcg
    52321 ccatttgaac tttcaggagg gcaaatgcgt agagtagcaa tcgctggtgt gcttgccatg
    52381 aggccggaag tacttgtatt ggatgaacca acggcaggac ttgatcccca gggcagaaaa
    52441 gaaatgatgg agatgttttg gcgtcttcat aaggaacagc agatcactat tgtattagtt
    52501 acgcatttaa tggatgatgt agccaatttt gcagattatg tctatgtgtt ggaaaaggga
    52561 cgaatcgtta atagcgggga gccgcaagag gtctttcaaa acattgaatg gctaaaagaa
    52621 aaacaactag gcgtacctac tgccaccgag tttgcagaag aattaatggc taaagggatg
    52681 aactttgcta ccttgccttt gacagctgaa gaattagcag acgctatcgt ccattatgct
    52741 ggaggaaatg cccatgatga ataaattgat atttggtcgt tatatgcctg gagattcctt
    52801 gatccatcga ttggatcccc gagctaaatt attagccagt ttttatttta tcggaatcat
    52861 ttttttagca aataattggg aaacgtacct tttgttggca tttttcacat tactttctgt
    52921 cttcttgtca aaagtagatt tgaaattttt tatccgaggc attcgcccat tgatctggct
    52981 aatcctcttc actgtagctt tgcaggtctt gtttacaagt ggcggagaaa cttattggag
    53041 ttggggtgtt ttccatatca ctgaatttgg tgtgatgaac ggcctcttta ttttctgtcg
    53101 attcgtgttg atcattttta tgtcgactct actcacgctt actacaccgc ctttggaatt
    53161 atcggatgcg attgagtact tgcttcgtcc attgaaagcc gtacggttcc cagtccacga
    53221 aatttcattg atgctgtcga ttgctcttcg ttttgtgcct acattgatgg acgaaacaga
    53281 aaagatcatg aacgctcagc gcgctcgcgg tgttgatttt ggtgaaggaa acctagttca
    53341 gaaaatgaaa gcagtcattc ctttgctgat tcctttattt gtcagcagct tcaaccgtgc
    53401 ggaagatctt gcgactgcta tggaagcaag aggctacaaa ggtggggaag gaagaacgaa
    53461 atatcgtgta ttgcattggc ataatagaga cacgatggtt atcattgctt ttgtattatt
    53521 aactgtgcta ctgatctttt taagaggata aacgttcagt gggccgctat ctattctttt
    53581 cttgctgatc tgtttgcaag aaaggatgca agcagtgtat gacaagaaat attagggtct
    53641 gtggcagact aaggtcacag gcctctttct tttatctgaa aagaaagaac gaaaaaattt
    53701 tatagaaaaa tcattattcc agataacctt ttaggaacaa aggagagaaa aacatggtac
    53761 gttacaaagc aatcattgcg tacgacggaa caaattttaa tggattccaa aaacagccaa
    53821 atgggcgtac tgttcaagaa gaagtagaaa aaacattaca aaaaatggca aatggaaaag
    53881 aaatcacggt ctttggttcg ggaaggacag atgccggagt acatgcgatc gggcaagtaa
    53941 tccattttga ttatccagag gagcgtccgt tagaacgaat gcgtttcgcg ctagataccc
    54001 agtcaccaga ggatattgct gtaagacaag tagaaattgt ttcagaagac ttccacgcaa
    54061 gatatttggt caaagaaaag acgtatcaat ttcgagtgga tatagggaaa ccacgcagtc
    54121 cttttcgacg ccattatgca agttattttc cttacccact tgatttggaa aaaattcagc
    54181 gggcgcttcc tgatttatta gggactcacg atttcacctc tttttgtgca tcaggaagct
    54241 cgatagagga taaaatacgt acgatctacg aggctaagat ggaggtaaat gaaacaaaag
    54301 acgaattgtt gtttacattt aggggaaatg gcttcttgta taagatgata cggatcttag
    54361 taggaacgct tttgaaaatt ggcaatggcc gattgcctga agatagtatc ccagatatta
    54421 ttgcgaaaaa agatcgtaat gcagcaggac ctacagcaca ccctgaagga ctttatttgt
    54481 atgaagtgaa atatgaataa gaggttatga tagaagtggt cagcttcaag aaataagaag
    54541 gtatttacga aaattacttc tcaaaatttt gcaaaatttc ggcttatttt cgaagaagct
    54601 acttctggaa caccgtttaa ggaagaggtc gtgaaatcag cgttttgacc tgagtattag
    54661 agaaacaaac gggaaaatag ctcctcatat tttgccgttt gttgagctaa taccgaaggt
    54721 cagctgattg aacaccgttt aagaaagagg tcgtgagcct gtcatttaac tctgagtcac
    54781 aaagagcaat ttcaaaaaac agcttctcat ctttgatgaa tatcgtgact tgtggacgag
    54841 gcgttgataa gtgaatcatc tttttgttac ctattttttc tgtaaccaaa aaacgatgga
    54901 acgttgtttt gattgccctt ggtgcggaca gtctgaacag gttccatcgt ttctttattt
    54961 tctacgtctt cttctgtgtg tgtttcgtgg ttgttcttct attgctggat caatcgtgcc
    55021 ataaaagggg cgattgcggt caaggtacca gacataaaga ataaacaatg caatacatag
    55081 gacaaaaagg attgcaccta tcgtaaatcc ttcaggcaag taactgaaag tgacagtatg
    55141 tttacctgct ggaactttta tcatcaagaa tgcgtctttg aaattgctga ccttgactgg
    55201 tttcccatca attttagctt tccatccttt gtcataagga atagttgtca ccaacatgga
    55261 atcttcagcg gtatttacag tcccagaagc ttttctgcca gtggcttcca tttcgacacc
    55321 tttttctttg atctgattca ttgcggcttg ataggctaca acatctagac caacgacttt
    55381 aggctccatg aaacttattt ctttcgtccc ataaaaactt gcagtaaatg tcactgttgt
    55441 ctcttgagga taatagccaa gatcatagta ttgaccagaa atgttgattt gcgtttttcg
    55501 cgatgtacca ttgacagtga tcgtcgcact agaactttct aattgcgcaa aattggttgg
    55561 gaacaaactt aagtaagctt gtgtatttgc aggcacatgg acttcccaag tgagctcttt
    55621 tggctggtta tctgctgttt ctgagtaagt gacgccagtc gttgtctttg tgatcgtcgt
    55681 attcaactga ttcttcaaag taggtgtata gaatgtaaaa tactgttcat ttagaccaga
    55741 caatgtattg atcaagtttg tctgactggt caaattatct gtcactggct gctcaacatc
    55801 attaacggac aacggtgcag taaatgctag tggcaatgca ttgacattct gatacagtgt
    55861 atagtcgccg ctttgaccag cttggcggaa gccatattta ctgaacgtgc tttcgttttt
    55921 agaaatgtta tatttgattc ctgtaaaccc atccatcagc aaagtattat tgttataacg
    55981 gatattcaaa tttgtccctc ttgaacggaa acctaattta tccaggtaag aggaagaatt
    56041 acgatttctt atagaagaaa acaaactgat accactgtaa ccatagttga tcgaatcgtt
    56101 tgcagatact ggatccagat tttccaagcg gtaaaacgag tcggaatctt tttttgtttc
    56161 atttacaaga gctttcaagt caggataagg atcagtatat aaactgcggg aagcataatt
    56221 ccaatcatca agaatgccat ggatcatact gttcgtattg accagtgctt cagcagacat
    56281 gatcaataat aacaatatac tcagatgatt gaatgaaagt tttttatgct gataaaatcc
    56341 cacagcaaaa gcataaatcc ctaagaaaac cactgtcaat acaaaggaag tcatcgtcac
    56401 ataggtgtag cttccttcag gtttgatgcc ccatgccacc gcaaagagag cagctacgac
    56461 caaaatgatt cctaacaaca gcccacgatc gtcttgttct atcttttccc atccttttcc
    56521 tgcaagcagg agcactaaga aagagaacag ataggcataa cggaataaaa acatattcgg
    56581 tgcatgcata ccatgccaga acaaattcaa tggaacaaga taaaaacttg cgataagtat
    56641 ggcgaaaaga ctgccgtaca aaaatttatt tttcaacggt actttccgac tggcaaaata
    56701 aaacagacag aggactaatg gtagcagacc tgcgtaaacg aacggaatcg atccgtattt
    56761 tgtcgtgtca taaacaccaa tcatattttt catgattaga tccagataag aagtcgcttc
    56821 tgttttcaac gtagtgatct gactcagtgc ctcgccattt gaccgaagat ccagcactgc
    56881 tggcaataca atgatcattg aagcgccgcc tgccagtaaa gaagtgatac cataaggaac
    56941 aatcgttttt ttatattctg accagtttgt caatagacgg gcgataaaat ataagaaaga
    57001 aaatacgcca atcataaaac ccatataaaa actggataaa aacaagagta aataactgat
    57061 aaataaaagt ttcggccgtt taagatccat aacacgatga atccccaaaa tcactaatgg
    57121 aagataaaaa aatgcatcca gccacatgat gatctccgaa tgtgcagtga taaatgagat
    57181 caatgcgtaa ctgacgctaa gtgcaacatg cccccagcga ggaatcttga acgtacgtgc
    57241 aagaaaccaa aaagccaatc cggcacagcc gaccttaagc aaagtcagaa catacagtgc
    57301 atctggcatc atctggtttg gaaaaaagaa caccagtggc gtgaagatcc cgcctagata
    57361 gtaagaaatt aaagataaat aattcaatcc tagagaagca ttccatgtat aaaaaatact
    57421 ttgctttcca agaagaacat ttcggaagct ggcatgaaaa ttagaaaact gtgaaaaggc
    57481 atcacttgcc aaaatacttc tgctgcttcc aggatagatt ccgatactta gataaacaag
    57541 tgctaaaatc aaaaacggaa tcaggaaact agctgctgcg tagcgtccgt tttctctaaa
    57601 gaatgacttt aaattttttt tcatatttat tttccaccaa atttctatga agttgtattc
    57661 agtttatcat atccttgttt ttctctgctt aaaaagagtc tagttttcac aaaaaaataa
    57721 aatttagaat aaataacttc ataatttgtt cgtataacaa aaataaacgc aaaaaaaggg
    57781 taggaatcaa aagtcccacc cttaaaaatt tttattaaat aaaaaaccgt tcactatctg
    57841 aacggtccaa gtatcattca attaaacgcg ttcaactttt cctgatttca aagcacgagt
    57901 tgacacccaa acttttttag gttttccgtc gaccataacg cgaacttttt gtaagttagg
    57961 tttaacagta cgttttgttg agttcatagc gtgtgaacgg ttgtttccac tttttgtctt
    58021 gcgacctgta aagtaacaaa cttttgccat ggtaatttcc tcctttgcct tgatttggag
    58081 caatagaata attcagctca taccagacta acatatcata tagaatggga atttgcaagt
    58141 acattttcat agatacaatt gacaattgag aagaataatt gtaaattaaa caaggttata
    58201 cctgaaattt tgcaaggttt ttgagtactt tgggtaactg ccttttattt caagttgcgc
    58261 tatgataaaa tatgatagaa gaaaaatggg agttttttac ccgttaagga ggctcattca
    58321 tggctgtaaa aattcaaacg tcagctggaa ccattgaaat taccaatgat gtaattgcaa
    58381 cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt aggaatggcc agcaaaaatc
    58441 aaattaaaga taatattact gagattttgc gtaaagaaaa ttactcaaga ggagtagttg
    58501 tccgccaaga agataatggt attgctgtag atgtttatac gattatcagc tatggtacga
    58561 aaatctctga agtttcccgc aacgtccaag aaaaagtgaa atacaatcta gaggcattat
    58621 taggcgtaac tgccaattca gttaatgtct ttgtacaagg tgttcgtgta ctgccagact
    58681 aggcaggaga ctggctggta actcagcagt cgatctgaag gaggattatc taggtgagtg
    58741 taacagaaat aagtgcaagc cagttccagg aaatggtcca ggcaggtgcg aatcgtctgc
    58801 aagtaaatgc agaatacgtc aattcattaa atgtattccc tgtgccagat ggcgatacgg
    58861 gaacaaatat gaatctatct atgactagtg gagctaaggc agtagtagat tcaacctcag
    58921 aaaaagtagg agaattagca gcaatgcttt caaaaggatt gctgatgggt gcgcgcggaa
    58981 attccggcgt tattttgtct caattgtttc gtggattttc taagcaaatt ccagatgttg
    59041 tctcattaaa tgcttctgat cttgctgcag ccttcactca tggagttgaa actgcgtata
    59101 aagcagtaat gaaaccagta gaaggaacga ttttgacagt agctagagaa gcagcgaaag
    59161 ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga agtaatgaca gctgttgtaa
    59221 aaggagcaaa gcgtgcttta gctaaaactc cagacttgct tccagtccta aaagaagtcg
    59281 gtgtcgtaga cagtggaggg caaggactgc tgtttgttta tgagggcttt ttaagtgcat
    59341 tgaatggaga attccaagcg gatgaaacat atgaaccttc tccagcagaa atggatgata
    59401 tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat ggcgacagaa gatatccatt
    59461 ttggttactg tacagaaatc atggtaaaaa tcggagaagg acctacagtc gatagtacat
    59521 tcgattacga agaattccgt aactatttag atggtatagg agattcttta cttgtggtca
    59581 atgatgacga gatcatcaaa gtccacgtgc atacagaaaa cccaggagaa gtcttgaatt
    59641 atggacaaaa attcggttca ttagtcaaaa tcaaagtcga caacatgcgc ttgcagcatg
    59701 agacgatttt ggaacacgat gaagaagtga acgagtttgc gaaaaaagaa ataccacgta
    59761 tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt ccaagagctg tttgaaagtt
    59821 taggcgctaa ttatgttatc agtggtggac aaacaatgaa tccaagtacg gaagacattg
    59881 ttaaagcgat tcaagaagta aacgcagata aagtgattgt cttgccaaac aataagaaca
    59941 tcttcatggc agctgatcaa gcagcagaag tagcagatat cccagtagca gttgttcctt
    60001 caagaacgat ttcccaagga ttgacagcta tgctttcatt caatgatcaa gcttctttag
    60061 aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt tgtgagcggt caagtcactc
    60121 acgcgatcag agatacctca attgacggtg tagagatcaa agaaggagac tttttgggca
    60181 tgatcgatgg aaaaatcctg atctctaacc cagatatcac agcaacagcg ttaagtacac
    60241 tagaacaaat gatcaatgag gacacagaga tcgtaacgat cttggcaggt gatgaaggaa
    60301 cagttgaaca agcaaatgaa tttgctgaga aactaacaga aaaacatcct gatttggaaa
    60361 tcgagatcca ccaaggagat cagcctgtct atccttatct attttctgct gaataatttc
    60421 tgtgtcagaa tatctttcta acacagtatt cattcataaa aggccttcgg ttagtgatca
    60481 ggtatgatcg ctgaccaagt cctttttttg ttaaaggagg gaggaaaaat ttatgcgttc
    60541 tttagatgat ccaatcacta tgctggcagg agtcggagaa aaaagagcag ctagtcttgc
    60601 cagcttgggc atccaaacga ttgaagactt actgacttat tatccttttc gctacgaaga
    60661 tattcaggaa agaaatctaa acgagatcca ggatcaagaa aaggttactt tgaaaggaat
    60721 cgtagtctcg cctcctgtga tgaatcgatt tggttataaa aaaagccgat tgcaatttcg
    60781 tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt aatcaacctt atctaaaaga
    60841 aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa tgggatgcaa agcgtaaagc
    60901 cttgaacggg atgaagattt taggttctca aacaatggat gacttttcgc cgatatatca
    60961 cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta atcaaaaaag ggtttcaaga
    61021 atacggcaca ttgattgaag aaaatctgcc aaacaacctt gtggagaaat atcgactgct
    61081 tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa gaccacgaag aaaaccatca
    61141 agccaaacga cgtgtcgttt ttgaagagtt ctttttattt caaatgcgtc ttcaaggatt
    61201 gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc ctttatgatg tccaaaaatt
    61261 gaaacaattt actcaaaagc taccttttga actgacaaat gcacaaaaac gtgtaacgaa
    61321 tgaaatctgt cgggatcttc gcagtccgca gcatatgcag cgacttttgc agggagatgt
    61381 aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac gctaccgtta cagcaggttt
    61441 ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa caacatatgg aaagcttgca
    61501 acagctgttt gatcccaatg aagtaaccat cgcattgatt acggggtcaa cgaaagcaaa
    61561 agaacgacgt gagctccttg aaaaactaga gcaaggggaa attaacgtcg tgatcggcac
    61621 acatgcattg atccaagatg gagtcgaatt tcagaattta ggtctagtta ttacggatga
    61681 gcagcaccgt tttggcgtga accagcggaa agtacttcgt gaaaaaggct ggcgaccaga
    61741 tgtcttattt atgacagcga ctccgattcc tagaacatta gctattacag catatggtga
    61801 gatggatgta tctatcattg atgaacttcc agctggacgt atcccgatcg aaacacgctg
    61861 ggtaagaaca cctcaattgg acagtgtact ggattggacg aaaaaagaac tggcaagagg
    61921 acatcaaatg tatgtcatct gtccgctgat tgaagaatca gaagcattag atgtaaaaaa
    61981 tgcagtggaa atctatgaaa aacttcgtga actatttgca ccaaaatatg aagttggctt
    62041 gttacatggt aaaatgaaga atcaggaaaa agaagtaatc atggaagcct tcaaagacaa
    62101 ccagatgcag atattagttt cgactactgt aattgaagta ggggtaaacg tgccgaacgc
    62161 gaccgtcatg ctgatcatgg atgcggatcg atttggattg gcacagctgc accaacttcg
    62221 tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc ttagttgcta atcctaaaaa
    62281 tgaactaggt gtggaacgga tgaaaatcat gacagagacc accaatggtt ttgtactaag
    62341 cgaaaaagat ctggaattac gtggtcctgg tgaagtattt ggcttccgtc aatctggtct
    62401 gcctcaattt gcgattgctg acttggtgac ggatggaaat gttttggagg tagccagaga
    62461 agaggctgca gccctttggc aaataaagga ttggcagatg cttcctgagt atcagccttt
    62521 agcgcaaagc ctccaaatag aaaatcagga taatcgttat ttcgattagc agactgtgct
    62581 atactttata ctgatgacgg aaaaaggagt ggaaacaatg aaaattgccg tagatgcaat
    62641 gggcggagat cacgcacctc aagcaattgt agaaggcgtg gctttagcgc agcaagattt
    62701 tccagaagta gaattcttac tttacggaaa agaagcagaa atcaagaaat acttaacaaa
    62761 tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc aatagtgatg atgagccagt
    62821 aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt gctgcacaag cagtgaaaaa
    62881 cggtgaagca gatgctgtat tttcagcagg gaatacaggc gcgctactag cagcagggtt
    62941 gttcatcgta ggacgtatca aaaatatcga acgccctggc ttgatgtcta ccttgccagt
    63001 agtagggaaa gaaggtgccg gtttcgatat gctggatctt ggcgctaacg cagaaaacaa
    63061 accagaacat ttgttgcaat acggtatttt aggttctttt tatgcacgta aagtccgagg
    63121 tatcggacgt cctcgtgtcg cactcttaaa caacggaaca gaagcaacaa aaggtagtga
    63181 agtgacgaaa aaagcctatg aactccttca gaatgaaaca agcttgaatt ttattggaaa
    63241 cgtagaagct cgtgagctac tcaatggtgt ggctgatgta gtggtcacag atggatttac
    63301 aggcaacgct gttttgaaat ctattgaagg aacagcaatg aatatgatga atctattaaa
    63361 atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc gctatgcttt taaaagacgg
    63421 cttacgttct ttgaaagcag aaatggatta ttccaaacat ggcggtgccg ttttgtttgg
    63481 cttgaaagca ccagtcatta aaactcatgg agcaacaggc cccgatgctg ttcgctatac
    63541 gatccgccaa atccacacaa tgcttgagac agatgtagtt ggacaattag tcgaacaatt
    63601 tgaaaaaagc gaagaatagt atttggatat ctgaagcgat ccgttccatt gaaaaaaagg
    63661 tggacattcc tgaaaaatgc cagtaaaatt agccatggtg taggtaattg acaagtgtat
    63721 gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt taacaaagta gcgaaaatta
    63781 tttcaaatca ttttgagata gataccgata aagtgacgga tgaattgaac atcaaagatg
    63841 atttaaaagc tgattcaatt agtattatgg aattcgtttt agagcttgaa gatgaattcg
    63901 gtacggaaat ttcagatgaa gacgctgaac aaattgaaac agtcggtggc gcagtagatt
    63961 atatcagcag ccatcttaaa taatttcaaa taaaagcaaa gaaggataaa aa
//
LOCUS       DAFFSV010000004        55395 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFFSV010000004 DAFFSV010000000
VERSION     DAFFSV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.004"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            39..1181
                     /locus_tag="LR110_000190"
     CDS             39..1181
                     /locus_tag="LR110_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL4821197.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(1279..2670)
                     /gene="sufB"
                     /locus_tag="LR110_000191"
     CDS             complement(1279..2670)
                     /gene="sufB"
                     /locus_tag="LR110_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBL4821198.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(2675..3145)
                     /locus_tag="LR110_000192"
     CDS             complement(2675..3145)
                     /locus_tag="LR110_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBL4821199.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(3132..4367)
                     /locus_tag="LR110_000193"
     CDS             complement(3132..4367)
                     /locus_tag="LR110_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL4821200.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(4364..5650)
                     /gene="sufD"
                     /locus_tag="LR110_000194"
     CDS             complement(4364..5650)
                     /gene="sufD"
                     /locus_tag="LR110_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBL4821201.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(5663..6436)
                     /gene="sufC"
                     /locus_tag="LR110_000195"
     CDS             complement(5663..6436)
                     /gene="sufC"
                     /locus_tag="LR110_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBL4821202.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(6633..7481)
                     /locus_tag="LR110_000196"
     CDS             complement(6633..7481)
                     /locus_tag="LR110_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL4821203.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(7505..8191)
                     /locus_tag="LR110_000197"
     CDS             complement(7505..8191)
                     /locus_tag="LR110_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4821204.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(8184..9218)
                     /locus_tag="LR110_000198"
     CDS             complement(8184..9218)
                     /locus_tag="LR110_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4821205.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(9281..9537)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9613..9951)
                     /locus_tag="LR110_000199"
     CDS             complement(9613..9951)
                     /locus_tag="LR110_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBL4821206.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(9953..10309)
                     /locus_tag="LR110_000200"
     CDS             complement(9953..10309)
                     /locus_tag="LR110_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBL4821207.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(10315..11502)
                     /locus_tag="LR110_000201"
     CDS             complement(10315..11502)
                     /locus_tag="LR110_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL4821208.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(11693..12538)
                     /locus_tag="LR110_000202"
     CDS             complement(11693..12538)
                     /locus_tag="LR110_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL4821209.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            12772..13737
                     /locus_tag="LR110_000203"
     CDS             12772..13737
                     /locus_tag="LR110_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBL4821210.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            14114..15157
                     /locus_tag="LR110_000204"
     CDS             14114..15157
                     /locus_tag="LR110_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBL4821211.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(15219..15683)
                     /locus_tag="LR110_000205"
     CDS             complement(15219..15683)
                     /locus_tag="LR110_000205"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBL4821212.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            15929..16876
                     /locus_tag="LR110_000206"
     CDS             15929..16876
                     /locus_tag="LR110_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL4821213.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(17087..19159)
                     /locus_tag="LR110_000207"
     CDS             complement(17087..19159)
                     /locus_tag="LR110_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL4821214.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(19092..19469)
                     /locus_tag="LR110_000208"
     CDS             complement(19092..19469)
                     /locus_tag="LR110_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBL4821215.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(19598..20176)
                     /locus_tag="LR110_000209"
     CDS             complement(19598..20176)
                     /locus_tag="LR110_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4821216.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            20308..21600
                     /locus_tag="LR110_000210"
     CDS             20308..21600
                     /locus_tag="LR110_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL4821217.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(21660..22778)
                     /locus_tag="LR110_000211"
     CDS             complement(21660..22778)
                     /locus_tag="LR110_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBL4821218.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(22792..24213)
                     /gene="arcD"
                     /locus_tag="LR110_000212"
     CDS             complement(22792..24213)
                     /gene="arcD"
                     /locus_tag="LR110_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBL4821219.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(24459..25580)
                     /gene="mnmA"
                     /locus_tag="LR110_000213"
     CDS             complement(24459..25580)
                     /gene="mnmA"
                     /locus_tag="LR110_000213"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBL4821220.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            25984..26583
                     /locus_tag="LR110_000214"
     CDS             25984..26583
                     /locus_tag="LR110_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBL4821221.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(26721..28007)
                     /gene="efmA"
                     /locus_tag="LR110_000215"
     CDS             complement(26721..28007)
                     /gene="efmA"
                     /locus_tag="LR110_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBL4821222.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(28278..28622)
                     /locus_tag="LR110_000216"
     CDS             complement(28278..28622)
                     /locus_tag="LR110_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBL4821223.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(28759..29904)
                     /locus_tag="LR110_000217"
     CDS             complement(28759..29904)
                     /locus_tag="LR110_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL4821224.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(30084..31058)
                     /locus_tag="LR110_000218"
     CDS             complement(30084..31058)
                     /locus_tag="LR110_000218"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL4821225.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(31138..32013)
                     /locus_tag="LR110_000219"
     CDS             complement(31138..32013)
                     /locus_tag="LR110_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBL4821226.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            32143..34050
                     /locus_tag="LR110_000220"
     CDS             32143..34050
                     /locus_tag="LR110_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBL4821227.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(34591..37869)
                     /locus_tag="LR110_000221"
     CDS             complement(34591..37869)
                     /locus_tag="LR110_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBL4821228.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(38140..38685)
                     /locus_tag="LR110_000222"
     CDS             complement(38140..38685)
                     /locus_tag="LR110_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL4821229.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(38697..39389)
                     /locus_tag="LR110_000223"
     CDS             complement(38697..39389)
                     /locus_tag="LR110_000223"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBL4821230.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(39408..39692)
                     /locus_tag="LR110_000224"
     CDS             complement(39408..39692)
                     /locus_tag="LR110_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821231.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(39705..40268)
                     /locus_tag="LR110_000225"
     CDS             complement(39705..40268)
                     /locus_tag="LR110_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL4821232.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            40496..41158
                     /locus_tag="LR110_000226"
     CDS             40496..41158
                     /locus_tag="LR110_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBL4821233.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            41282..42478
                     /locus_tag="LR110_000227"
     CDS             41282..42478
                     /locus_tag="LR110_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBL4821234.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(42579..43955)
                     /locus_tag="LR110_000228"
     CDS             complement(42579..43955)
                     /locus_tag="LR110_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4821235.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(43992..44858)
                     /locus_tag="LR110_000229"
     CDS             complement(43992..44858)
                     /locus_tag="LR110_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBL4821236.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(44947..45972)
                     /locus_tag="LR110_000230"
     CDS             complement(44947..45972)
                     /locus_tag="LR110_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL4821237.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(45990..46664)
                     /locus_tag="LR110_000231"
     CDS             complement(45990..46664)
                     /locus_tag="LR110_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4821238.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(46848..47741)
                     /locus_tag="LR110_000232"
     CDS             complement(46848..47741)
                     /locus_tag="LR110_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL4821239.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(47815..49512)
                     /locus_tag="LR110_000233"
     CDS             complement(47815..49512)
                     /locus_tag="LR110_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL4821240.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(49505..50818)
                     /locus_tag="LR110_000234"
     CDS             complement(49505..50818)
                     /locus_tag="LR110_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL4821241.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(50818..54855)
                     /locus_tag="LR110_000235"
     CDS             complement(50818..54855)
                     /locus_tag="LR110_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL4821242.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(54868..55326)
                     /locus_tag="LR110_000236"
     CDS             complement(54868..55326)
                     /locus_tag="LR110_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL4821243.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT        15465 a        11974 c        10020 g        17936 t
ORIGIN      
        1 cttctctacc tgcaataatg aagtaaggag agaatcaaat ggcaacattt tttagttata
       61 aattacaaaa tggcgaaaaa tattggggat ttgaaactta tttagggaaa gatcccaaga
      121 ctggaaaaga tatacgtaca aagcgtagaa gattcaaatc aaaagctgag gcgcaaacag
      181 cacttaatag actacttgta gactttaaag aaaaacaaga gtttaaaatc aatgttacaa
      241 cttttcaaga gttgtatgaa ttatggattg ataactacag aacaagagta aaaccgtctt
      301 ctgttgccgt tgctcaacgt ttttgcaaaa accatatatt acctgctttt ggggaattac
      361 gattggataa aataagtgtt agctattgcc aaaaacaggt gaatgaatgg cacaaaacat
      421 taaaacaata tggtttcttg agaaaaacaa ctgctcaaat atttaaattt ggagtagcaa
      481 tggaagtctg taaggataat cctatgtcta aaacattgct tccaagaaaa attgaagaag
      541 aacaaccctt gaagttttac acgaaagatc aattacaact gtttttacag aatactaaag
      601 aaaataattc agttaaactg tacacttttt tccgtttatt agcttataca ggaatgcgta
      661 aaagcgaagc attggcgctt caatgggaag atatagacta tttcaataaa acaataacaa
      721 ttggtaaaac tatcgctcaa gacgagttta atcaagtagt tctacaagta cctaaaacta
      781 aaaattcatc aagaacgatt cagcttgatg attttacact taaacaactg cgtatttggc
      841 aacaagaaca aatgaaaata atgatcttat atggctataa cacgaattca ccaaaacagt
      901 ttcttttcac tacaaacaca aacaagttgt actaccccca agtagtaaac gattggttag
      961 actggattta caaaaagaca ccgatggagc ctcaaattac tccacatgga tttaggcata
     1021 cccactgtag cttattgttt gaaagtggtg cgtcgataaa agaagtacag gaacgattag
     1081 gtcataaaga tattaaaact accatgaata tctatgctca tgtcacacca caatctgtta
     1141 agaaaactgg tgaccgattt gcaaaattta tgggcgaata attccaaacg tagccaaaaa
     1201 cgtagccaac cattaaaata caaataaaaa aacgctcgaa acgctttaat atcaacgttt
     1261 cgagcaatga aatagttatt aacctacaga cccttccatc tcataactga tcaatcgatt
     1321 gagttcgact gcatattcca ttggaagttc tttggtgaat ggttcaacaa agcccatgac
     1381 gatcatttca gttgcttcag attcggtcag gcctcggctc atcagatagt agagttgttc
     1441 ttctgagatt tttgagactt tggcttcgtg ttctaaagag acttggctgt tgtggatctc
     1501 attgaatggg atcgtatcgg atttcgacca ttcgtccatg atgatcgtgt cacattcgat
     1561 atgagagatc gaacccgcac tgtttttggc aaaggttact tgtcctcgat agtttacttc
     1621 tccgccgtct tttgaaattg atttagagac gatagagcta gatgtatttg gtgcattgtg
     1681 gatcattttt gcacccgtat cttggatttg gtttgctcct gcaaaggcga tggagagcat
     1741 ggtgcctcgt gcaccttttc catctaagta aacacttggg tatttcatcg ttgtttttgc
     1801 acctaggttt ccgtcgatcc attcaacggt tgcgccttcg tatgcttttg ctcgtttggt
     1861 tactaagttg tacacattgt ctgaccagtt ttggatcgtt gtgtaacggc aatacgcatc
     1921 ttttcttgta aagatttcta cgatggcggc atgcaggctg ttacttgaat aagtaggtgc
     1981 cgtacaacct tcgacatagt ggatgcttgc tccttcgtca acaatgatca gcgtacgttc
     2041 gaattgcccc gtgttttccg cattgatccg gaagtacgtt tgtaatggaa catcgacttt
     2101 gacgcctttt ggcacgtaaa taaaggtccc acctgaccat acggctgaat tcagcgctgc
     2161 taatttgttg tcggttggcg gtacaagttt tgaaaagtac tctttgaata attcagggta
     2221 ttctttcaaa gcagagtcag tgtcagtaaa gacgattcct aacttttcga attcatcttt
     2281 catattatgg tacacgactt ctgattcata ttgtgcggaa gcaccggcta gataagctcg
     2341 ttcagcttct ggtataccaa tcttttcaaa tgtatctttg attttttctg gtacgtcttc
     2401 ccagtcacga gcgggtttgt cacttggttt ttggtaatat ttgatggcat cgaaatcgat
     2461 gtctgaaagg tcggggcccc atgtttgcat tggcattttg ttaaatgttt ctagagattt
     2521 cagacggaat tcaagcatcc attctggttc gcctttgatt cttgacatct cacggatcac
     2581 ttcttctgtc aagccttctc ctgtactgaa aaccggttcg acatcatcgt gaaacccgaa
     2641 tttatattct tctaattctg gtacgcccat gttctcactc ctcttccttg tggtaatgat
     2701 ggaaagctgc ggtaccatcg ttttccattg cttttcctag tgctttccag gcaagtgtcg
     2761 cacatttgat ccgagcggga aatttagcta caccaccaag catagcagca tcgcctaatt
     2821 ggtcgggatc tttgacttct tttccttgga caagctgtag aaagtctaca gttaaatgtt
     2881 ctgcttcttc tattgatttt ccgatgacgg cgtctgtcat catcgaggca cttgccgtac
     2941 tgatggagca gccactgccg tcaaaggcga tatttttgat gattccgtct tttacatcga
     3001 cttgcaattc gatcacatct ccacatgtgg ggttgttcaa ttcgatcttg cttgtcgagt
     3061 cagtcaagct tccacgatga tgcggatggg aagaatgatc caagatcact tgacggtata
     3121 aattgtctaa tttagatagt cccatgttgg aaaaactcct ttgttgccat gattgcttca
     3181 actaaacggt cggcgtcttg cgttgtgttg tagaaataaa agctcgcgcg agcagtcgct
     3241 gatacttcaa ggtacttcaa tagcggttgc gcacaatgat gacctgctct gacggctacc
     3301 ccttccatgt ctaaagctgt cgcgacatca tggggatgta acccatccaa attgaatgca
     3361 acgactcctg tccgaaaatt cgggtcttgt ggaccataaa cagtcaatcc atcaattgct
     3421 tgtaattttg gcaacacata agcggctagt tcttcttcat atcgatggat attgtccata
     3481 ccgagttttt gcaaataatc gattgcatgg cctaatgcga tagcgccggc gatatttggt
     3541 gtccctgctt cgaatttcca aggtagttct ttccatgtac tgtcgtataa ttcgacaaaa
     3601 tcgatcattt cgccaccaaa ttcaacaggt tccatttctt ctaaccattt tcgtttacca
     3661 tataaaacac caatacctgt tggtccgcac attttgtgtc cgctgaaagc aaagaaatca
     3721 cagtctagtg cttggacatc aatggccatg tgcggggcag attgagcacc atctactacg
     3781 atgaccccgc cgttttcatg gacaagttca gctaattctt gaacaggatt gaagacacct
     3841 aagacattgg atacatgagc aatggagacg attttggttt tgtccgtaat ctttttcgct
     3901 gcatcctcca tatccaggaa gccttcttct gttagttcga tatatttcaa gacagcacct
     3961 gttcgttttg ccaactgctg ccacgggata acattagaat gatgttccat gtaagagagc
     4021 actatctcgt ctcctgcttt gatatagcgg tcgccgaagg ttctggctac ccaattcaag
     4081 cttgtcgtcg taccacgagt aaacagcact tctgctgttt cttttgcatg gatgaacgcc
     4141 cgtacttttt ctcttgcttg ttcataatca tgggtcgcgc gttctgctaa cgtatggaca
     4201 ccgcgatgaa cattggcgtt atcgtgttcg taatagtacg ctaatgcctg gagtacttgt
     4261 tttggctttt gggtcgtcgc tgcattatcc agataaacta aaggttcatc attgacgtct
     4321 tgaaataaga taggaaaatc attgcgtagc tttttagcat cgatcatcca ttcaacttcc
     4381 cttcgattac ttcgactaat tctttccgaa cttgttctac agggattgct gttaagacag
     4441 aaccgaggaa tcctcgaatc actaagcgct ctgcttcttc tttatgcaat cctctactca
     4501 tcagatagta catttcttct ggatcaactc gtcctacact ggcagcatgt ccagcagtta
     4561 cttcgttttc atcgatcaaa agaataggat tcgcatcacc acgtgcttta tcagaaagca
     4621 tcaagacacg actttcttgt tgggcatctg caccttttgc ccctttcaag atgtggccga
     4681 taccattaaa ggtcaatgtt cctttttctc ggatcactcc gtgttgcaaa atatgcccga
     4741 tcgtatgagg ggctttattg gttactcgtg tatcgattcc ttggatttgt ctgcccgagc
     4801 tgattgccac gattttgact tctgcgtgtg caccttctcc tgctaaatct gagtcaaagt
     4861 cagcgatcac atgaccgtca ttcatgacac caattgccca gtcgacagaa gcatctcgta
     4921 aaatgtgccc acggcgattc atataagaag taatattttc tcctaattga tctacggctg
     4981 aatatttgat ttttgaacca gctttagcga tgacttccac gatgatatta cctgatgatt
     5041 ttgctacttg ttctttggtt gtctggaaac gttccagata agaaaattca ctatgttcat
     5101 ctgccacgat caacacatgc ttgaagaagt gctcatcgga tgcgccatct tgaatgaata
     5161 aggactcgag tggttcatcg atcacgacat ttttaggcac atacaggaaa ataccgctgt
     5221 tcataaaagc tacatggagt gcagtcaatt ggtcttcgtt catgtttact gcttttttca
     5281 tataatattc ttggactagt tctggatatt cgatcattgc agtgaacaag tcggtaaaaa
     5341 tgactccttt ttctgctaat tccacaggca gttgttcaaa aatcgtccat gaaccttgtt
     5401 ggacaagcac aggattgtct ttcattgcat caaatgccgg aatcgtgcct gtttcactga
     5461 tggtttgtgt ttctttcaca tcaaataacg accagcgatg gaatttcacc cgatcgatat
     5521 gcggcaatgg cagttcatct gctttcgcta gtgcgtcttg acggaaggtt ctcatccaat
     5581 caggttcgcc tttacttaaa gaaaaatcat ttaccgcgtc aagataatcg agccagtttt
     5641 tttctttcat tcttgctccc ccttacgctt cttcttcttt atagtcgatt cctaattctt
     5701 tactgatacc agcgtagcct tctgcttcca gacgtttagc taggtcagca ttccctgtca
     5761 tgactactcg gccttccatc atgatgtgaa cgacatcagg tgtgatatag ttcaataatc
     5821 gttgatagtg ggtgatgatc aatgcaccga aattgtctcc gcgcatttca tttactcctt
     5881 tagcaacgac tttcaaagca tcgatatcca gacctgaatc gatttcgtct aaaattgcaa
     5941 atgtaggttc taacatcaac aattgcagga tctcattccg tttcttttct ccgccagaaa
     6001 aaccttcgtt cagataacgt tcagccattt cttctggcat atttagtaaa gccatttttt
     6061 catctaactt tttcaaaaat gccataacag aaattttgtc gttttcatca cgtttcgcat
     6121 tgatcgctgc acgcatgaat tccgcattcg tgatacctgg tatctcactt ggatattgca
     6181 tagctaaaaa taagccaagt cgcgcacgtt catctacttc caagtctaaa acatttttcc
     6241 cgtcaaacaa aatttcgcct tctgttactt catagtttgg attgcccatg atagcggcag
     6301 ataaggtaga ttttcctgtg ccgttaggtc ccatgatggc atggatttct ccggttttca
     6361 ttgttaggtt tacgccttta aggatttctt tttcctcgat cgacacatgt agatttttta
     6421 tttctaagac agacatgcat tataccccca aattctcttt cgttctcact cattttagac
     6481 taattgagaa tgaagcgcaa tgaccttcct tactaaaatc aaatatcttt tatttattat
     6541 taaacaattt tgatttctaa attagaatag gtataaaaaa gatgagaaat aagccattac
     6601 agacttattt ctcatctttt ttgttccata ccttattttg catcagcctt tactggttta
     6661 acagagccgc cccatttgtc tgtgatccat tcttggactt ctttgcggtg aagcacttca
     6721 atcagttttt tgattttgtc actgtcttca tcacctttac gagtcgcaat aatattcacg
     6781 taaggagagt tgtccttttc aagcaatacg gcatcttcta atggattcaa ccctgcgccg
     6841 taagcgaagt ttgcgttgat tgcaactagg tcaccttcat cattttggta cgtagaagct
     6901 aaaagtgatg ggtcgatcgt atggttgaac ttcaagttct tagggttttc ttcaatatca
     6961 tcgaatgttg ctgtttccag atctactcca tctttgactt tgactaagcc agcatcttgt
     7021 agaattgtga tgatgcgtcc ccagtctgat tcagaatttg aagtaatgat tgttgcacca
     7081 tcttttaatt cggaaacatc tttgactttt ttagagtaca agcccattgg ttcgatgtgg
     7141 acagcaccgg cattgacaaa gtcatatcct ttttctttga tttctttgtt caaataagga
     7201 atatgctgga agtaattggc atcaatgtcg ccttcttcca aagattggtt cggtaataca
     7261 tagtcatcga atttgacgat ttcaagatcg attccttcat ctttcaataa tggtttcaca
     7321 tgttccaaaa tctctgcgtg cggtgtcggt gaagcgccga ctttcaatgt cgtgtcgtct
     7381 gctttatttg ctgaatcttt gctgtcgctg ttgttaccgc atgctgctaa tcctacagtc
     7441 agcaaaagcg ctgctgcaaa tccaaaaatt ttctttttca tatcctacat ccccctatat
     7501 cctattatct tttgtctgtt ttcttagtta agaaatcacc gattccttga acgataaata
     7561 cgattagtaa aataattaaa gtcgcaacga atgtcactgt tagattacca cgctggtagc
     7621 cttcctgcca agcaagacct ccaagtccac ctgcgccgat cgcgccggcc atcgcagtaa
     7681 atccgatcat cgagatcgtt gtaactgtta atccagaaat caatgcggga atactttcag
     7741 gtatcaagac cttccagatg atttcccagt aacttgctcc cattgcatct gctgcttcca
     7801 gtacaccagg gctgacttca cgaaaagcaa tctctactag acgggcataa aatggtgctg
     7861 cggataaaac aagagctgga atggctgctt gcgcacctag catagtgcct acgattgctt
     7921 tagtaaatgg aatgatcaaa accatcagga tcataaatgg cgttgaccgg aaaatattac
     7981 tgatgatcga tacgattcca taaatgattc gagcatacgg tttgtttttg cgtccaatag
     8041 agtaaaggat caatcccaaa actaatccga tgatcgtcac taagaccatt gagattaatg
     8101 tcatatatag tgtatcaagg atagcctgat tcatcgtttc cgggttgatt tgtttaaaat
     8161 ctagatatgt ttctgaaaag cttttatcca cgatggatca cctcaattcc tactccttgt
     8221 tctttgatga acacttctgc ttgatctaat tctttggttt caccagtcat caagacagtc
     8281 aatgaaccaa aggaaccttc tttcacttgt ttgatctttc catacacaac gttgacatcg
     8341 atgttatagc gtcgaatcgc ttgggaaatg actggctcgt tggcatttct ttcgctaaag
     8401 gtcaaagttg ccaaacggcc gcttggattt tctttgatga gttcagctaa tagttctgtc
     8461 gaatcagctt ctggttcgat gtcttgttgt acaaagcgtt tggttacttc ttttttcggt
     8521 tgtctgaaga cagtcaatac gtctccttct tctacgactt tccccaactc catcacagcg
     8581 actttgttgc agattttgcg gatcacattc atttcatgtg tgatcaagac gatggtcaaa
     8641 ttcaatttct tattgatcgc taacaataag tctaatactt cttctgttgt ttgcgggtcc
     8701 aatgcgctag tagcctcatc acaaagcagg atctcaggat catttgccaa tgctcgtgcg
     8761 ataccgacgc gttgtttctg tccgccggaa agttgtgaag gatatgcttg tccgcgtcct
     8821 tctaacccga ctaaacgcaa cagttcttct gcccgttctt tacgtttgct ctttggtacg
     8881 ccagctaatt ccagcggcag ctggatattt tcaagtacag ttcttgacca taacaggttg
     8941 aaatgctgga agatcatacc gattttcttt cggaatgtac gtaattcttt gttttttaat
     9001 tttgtaatat cttgatcgtt tacgatcact tcgccgctcg tgggaagctc aagtccattc
     9061 aggagacgga ctaatgtact ttttcctgcg ccagaatagc cgacgattcc ataaatatcc
     9121 cctttttcaa cgtttagtga aacgtcattg acggcttgga tgtttccgtt tttcccattg
     9181 aatgtctttt tgacattcgt tagttggatt aatgacatgc cattcacatc tttctcttat
     9241 aatttttcaa acaaaaaagc cttcgccctc ttgccttaag caaaaggacg aaagcttgaa
     9301 cttccgtgtt accaccttga tttaccgtta cttcgcaata acagcctctt ccagtacttg
     9361 gacttagctt ctttccgtat actgtcgcgc tgtaacgggc actcccgtag taagtttgct
     9421 tacgctcgct tactctgctc taagaccatc ttccatctca ttttcgttac ccattttcag
     9481 caaacagggc tctcttcaaa cgtccgtgac atgtactctt cttttcaaag ctttctcgtt
     9541 tcatatagga ttgcttatga ttatagcaac tggaagaatc agtgtcaact tttttcaagt
     9601 gattttctga ttttataatt cgtcaaattc ttttggatca acatctttga aacttaagat
     9661 ccacgcattc atttcatctg aatcattcaa gatattgata tcttcaccga ttttatcatt
     9721 aacagaagaa actgtacccg ttagcgggct ggcaaattca ttgaccgctt tttccgcttc
     9781 aacttcgatc agaggttcat ctttttttag cgattgtcct gcttttggca gattggcaaa
     9841 cgtgatgtct ccaagttctt cttgcgcttc atttgtcaaa ccaacacagt attcttttcc
     9901 attaaataag atccaaaggt tgtccttttt cttcaaatgc ttgcctgcca ttttatatca
     9961 gttctccttc ataaattttt tcattgaatc caattgcaac aagctggtcc tctcgtaaga
    10021 tcagtggtcg cttgatcagc ataccatctg tcgataagac ttggctcgct tttgaagccg
    10081 taaactcgcc tacttgatct ttcagtccaa gttcccgata tttcatcccg cttgtgttga
    10141 acaggcgacg gattggtact tgactcgttt ctagccaagt ctcaatcgtt gaggccttcg
    10201 gtacttcttt gatcatatca acggtttgat aactaacgcc atgctggtct aaccacgctt
    10261 tggctttttt gcatgttgaa catttcgggt accagtaaaa ggtgtacaat tttcttacct
    10321 ccttgaagat cttgcaggtg cttcattttg gtatctcatc gataatatca acccgatacc
    10381 gatcatgttc cctaaaatcg aggatcctcc ctgactaatg aaaggcaatg ggatccctgt
    10441 taaaggaagt aatccgatat ttgctccaat attctcaaag acatggaaca gcagcatcat
    10501 gatcaaaccg cttgcgatat aggcgtaaaa ttcattattc gtatcgaaac acacccgaat
    10561 catacgatag attaaaataa aatacaagaa aatgacgaat gcaccaccga taaagccgaa
    10621 gttttcaccg ataacggtaa aaatcatatc ggactcacga acaggcacat agatattgct
    10681 tacgttgaat cctttaccaa acaagccacc ggtaccaatt gccaaaagtc cttgggccgg
    10741 ctgataactc attcctgacg tatcatggaa agggtccagc catgaatcaa tacgtgcaaa
    10801 ctgataagat ttgaatccaa ctttataaag caagtctctt ccgccttcag tcgtaaccaa
    10861 gaagatcgtt cctgctccaa cgactactgc caagattact acaggtgcga tgatgcgcca
    10921 agagattcca gacatcagga aaatcccgcc aaagatcgca agaaagacaa gcatcgttcc
    10981 aaagtcatct tgggccatga tcaatgctaa aaccggtatt gtcacgatca agagcttccc
    11041 aatcagccaa aagtccgatt tcatcgtccg ttcttttact tgcgtattat gtttagttac
    11101 gatcagtgcc atcatcaaga tgtacgcaat cttcatcaac tctgctggct ggaaagtaaa
    11161 cgcaccgaat cgaaaccagt ttttagaccc tgttgaagca gctaaattct gatcatagaa
    11221 tttgagcaag agtcccatta ccactaaccc caaaccataa aggtaaggag tcagtttcca
    11281 gatccatttg gaattcaaat gcatgatgac aacgattgct gccgctccaa ctaaatacca
    11341 gacaacttgc atccctacgc ctttcaagac tgctggattg ttcggatcat ggctaagtgc
    11401 tacatataat gagagcagac caatcaaaca taacagaaag actggtaaaa tcacgccata
    11461 atcaatacga ttatcgttcg aagcttttaa ttttttttcc atgtttcatc ccttttctta
    11521 aaaaccatac tgctgattat tatagcggtc cagttagata atggaaagtc tttccataga
    11581 catttgggaa aaattaactt tccgtttgtg cagttgcttg agcaaatgca gtacaaaagc
    11641 ctactgctta tctttagtgt atcatagaac tttttttgat acatgcaatt atctaccagc
    11701 tgagattctc ctcgctgtga gaagtttctg tgctttcatt gtatcgaagg atctcttcgg
    11761 caatctctag cagctgatca gaaaccagat tggttcgttg gatgaaataa aaatatcgat
    11821 ggaaattcaa cggagtcatt ggcacatttt ctggaacaga gcgcttagac aaaatcgagc
    11881 gaccgattcc ttccttcagc aacgcaacaa ttacatcgtt acttttcacg atcatcttct
    11941 tatcttggat attcatctct tcaaaataac gctgcgtgta atgaaaaaca ccagagtttg
    12001 cttctcttac aagccacaat ttgctttttg ggtccccagc gatgactagc tggtcttcca
    12061 tcaaagagta tctttgtacg ccggaagtct ctaatggctt ttcgataaaa ccaaaatcgg
    12121 attcatgttt cgctacttgt tctaatgatt catgagaatt cgtgatttcg atctcaaaat
    12181 gcactttagg aaaccttttg atcagatgtt tcatcaactg aggcaaaaga tagacagcaa
    12241 aggtgtggga agcaacgatc cgacatactt cttcttcatt ttgttcattg agcaattttt
    12301 gctgagtttc ttcccattct tctaacaaaa ccagtgtccg ttcatataat aagtcggctt
    12361 gcttagttgg aagcatctct tttcttccgt tacgttgaaa aagatcgata ttcaattccc
    12421 gctccaactg tttgatttga ttcgagactg ctggttgaga taaaaacagt acttctgctg
    12481 ctctggaaaa atttcttgtt tcatatactg ctcgaaaagt cgttaataac ttaaacatgg
    12541 caccacttcc ataacttttt atgataatta ctataaaaac tatttgattt aaatatacaa
    12601 aaaaccatct ataatagaaa tgtacaacaa aacagttgct gatccaacta gtaacttaga
    12661 tgaagacaat cagctgtctg cttgctcgta atcatacatt cctattcatt ttacaatgta
    12721 ttcgttcatc ttggtactaa aatctattaa ataaaaagga gaatattata tatgaagaac
    12781 tttttgaaaa ttattccgat cccgatctgc gggttgatcc ttgggttagc ttcccttgga
    12841 aatctgttta aagactatca ccatgttgct ttaggcaatc taactggcgg tatctctatg
    12901 attcttatga tattcatttt tgttaaattg tttatcttat tcgaacacac gaaacaaacg
    12961 ttgcatgatc caatcatcgc ctccgtttca ccaactttta cgatgtcact catggttatt
    13021 tgtacttatt ttgtttcata tgaaagtatt gcaccaattg tcaaattcat ttggctgtgc
    13081 gctgttatct tccaagtgat ccttgtcttt tatttcaatt atcaccatgt tatcaaagct
    13141 gatctttcaa ttgaagcaat ttatccaagc tggtttatca tttacgttgg atttggggtc
    13201 atcactgtaa ctgccgggaa cttcttccca cttattggaa aaattttctt ttggatttca
    13261 ttgatctgct atgcgttctt attgccgatc atcatcaaac gtgtcttttt cgtaagaaat
    13321 atggctcatc cgacacgtcc gctgattacg atcgttgcag cacctggttc tctttgccta
    13381 acaggttatc ttaaaaactt tgctgaacct aatttttatc ttgtcgtagc attgttcgct
    13441 ttatctcaag tattgtattt cgttgtgctg tctatgcttc cacgtttgtt caagcttcgt
    13501 ttttatccaa gctatgcagc ctttacattc ccgcttgtta tctctgcaac agctatattt
    13561 actactgttc attacttcaa cagtttaggt atctatagta gcgtattgga tttcttaaaa
    13621 gttgcggaat gtctgtttgc aagtgtagtt gtcttctacg ttcttgctca ttacgtacgt
    13681 ttcttaatta aagaacatag caaacatgga aaatttgttt ctaaaaaaat cgcttaataa
    13741 tcgattcaat aggttgtgaa aaagctgttc tgcatcaagt aataagaacg gccaaacaaa
    13801 aacagctgtt catgtttttg tactggttgg gcttattacc gcagatgcaa gcttttgaac
    13861 accgtttacc aagaggtcgt aaaagaagtg gtcagcttca agaaataaga aggaatttac
    13921 gaaaattgct tctcaaattt ttgtgaaatt tcggcttatt tccgtagaag ctacttctgg
    13981 aacaccgttt attcgaaaca agaggtcgta caagactttt gtcacgacct cttgttcttt
    14041 tgtcaaaata ctggtttagt gctactatgt tcgagtatgc ttttagtatt tattgacgaa
    14101 aggaaccgat caaatgaaat cattgtcaca tccaataaaa tctgtaaagg attcagcggt
    14161 ccgcgaaatc ttacccggtt tactgctttc ttttgccatc gctgccttca gcaggcttct
    14221 tgctatttat ctgccacagt taggtggagc gactattgct attttattgg gtattttatt
    14281 aggaaatact ttttttaaac agcctgttct agcaatcggc acaaaatttg ctgaaagcaa
    14341 actattagaa ttctctgttt ttttactagg tgcaactgtc acagctcaaa cgattgctca
    14401 aattggtgta aaaggaattg tttttgtgtt actgcaaatg tcagtcacta tcatcggtgc
    14461 ttacttgatt ggtaaaaaac tgcaattcag cgatacttct tctttgatga tggctggagg
    14521 aaacgctgtt tgtggttctt ctgctattgc ttcaatcgcc ccttccatcc atgcgaaaga
    14581 ggaagaaaaa ggacagatca tcacactagt caatctactg ggtactgtga tgatgttgac
    14641 tctccctttt cttggattac agttgtttgg tggaaatctc attgaaaaaa gtgcactttt
    14701 gggtggtaca ctccaatctg ttggtcaagt agtcgctggg gcaagcttac ttgacaattc
    14761 tgttgtcgaa ttcgccatgc tatttaagat tctacgtatt atgatgctcg ttgttgttgt
    14821 ctatctgttt gaaaaattca tgcacaagaa acaaccagct ctgtttgaaa cgccaactga
    14881 tacgcagaaa aaacgaaaat taccgcttcc ttggtatgta ttagggtttg tgatcttttg
    14941 tttggtcaac agcttgtttt ctttacctgt agttattgac caatcggcac attttatcag
    15001 cagctggttt gagatcactg ctttagcggc aattggtctg cgtttagact ttgcaaaatt
    15061 catgaaagaa ggtacacgct tcttatctta tggattagct gtcgggcttc tccaaacagt
    15121 gatggcttta ctatttattt ggctgatcaa agtttagaat gaacatgaaa aaacagttgt
    15181 gattctcgca tggaacaatg tccgaatcac aactgttttt aattttgcag atatccttct
    15241 aacaacaaca attggcgttt gatttcaacc ccatctattg cgtttcccat atatcctgtc
    15301 agttttccat tttgcccgat cactcgatga caaggaataa cgattggcaa tggattggtt
    15361 cgatttgcct gcccaatcgc tcgcacagct ttcggactat caacagcaac ggctacttct
    15421 ttatacgttc ggatttctcc ataaggaatg gtcgacaaag ctttccagac attttgctga
    15481 aaaggcgtac ctctctttat agataaagga acatcaaatt cttttctttt tcccgcaaaa
    15541 tattcttcaa actgttcttg tgtgcgcgtt aaaattcgct gattcgctga ttcgctttct
    15601 gaaattggct gaaaagatac agcagtcaat ccggattcat ctgcatctaa ccataaaaag
    15661 ccgataggtg tgtgtatttt cattttgttt gctcctagtc aatagactgg aacttcctcc
    15721 tttttctcta tccaaaacta cttgccaact aataaaaatt gttcttaaaa gtttgataaa
    15781 ccgaggaaac atggtaaact aaatacgtta gtttacaaaa agaatacctg ttcttttacg
    15841 taaaatcaaa ttttaaaggt gttatttaag gtttattaaa ttttgaatgt aaaccatata
    15901 taaacatacg attgaaagga tagaaacgat gaaagtatca attgttgtcc catgttttaa
    15961 tgaagaaaaa actgttcctc ttttttttga tgaggttgaa aaattcaaag gtgcccatga
    16021 gttcgaatat ttgtttattg acgacggatc aaaagacggt acgttagcag caatcaagaa
    16081 gctagcctca gcttatcctt atgtccgctt tgtctcattt tccagaaatt ttgggaaaga
    16141 agcggctctg tacgctggat tgcaagcttc tacaggagat ttagtgaccg tgatggatgt
    16201 tgatctgcaa gatccgcccg aactgcttcc tgagatgatc aaccggatca ctacttctga
    16261 tatcgattgt gtcggcaccc ggcgttcgac tcgtgaaggc gagcctgcga tacgcagttt
    16321 ctttgcgaaa aaattctacc agttgatcaa taaaatcagc gatacagaaa tggtcgatgg
    16381 cgctcgtgat ttccgcttga tgactcgtca aatggtggat gccgtcctag aattaacaga
    16441 atacaaccga ttttcaaaag gattatttag ctgggtcgga tttaaaacag aatatatttc
    16501 ttatgaaaac cgcgaacgtg tggctggcga aacatcttgg tccttctgga aattattcaa
    16561 ctactcgatt gatggtatcg tgaacttctc ggatgcaccg ttaacgattg cttcatttat
    16621 cggcgctttg tcttgtgtcg gttctggtat tgcaattttg ttcattattt tgcgggcatt
    16681 gttgtttggt gatcctacat caggatggcc ttccatggtc tcgatcttcc tgttcattgg
    16741 tggaatccaa ttgttatgtc ttggaatcat cgggaaatat attgggaaaa tcttcttaga
    16801 aacgaaacag cgtcctgttt atatcataag agaaacagaa aaagtgaagc caaatgaatt
    16861 gaagcctcac caataatata cgtcaaatga agtaaacatt tttttaaatg aaaaaggact
    16921 gtgacaaaag tcttgtcaca gtcctttttt ccgaataaat ggtgttccag aagtagcttc
    16981 ttcggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
    17041 atttcttgaa gctgaccact tctatcacaa cctctttcat atcttattac ttcttcattg
    17101 ctgcttttag tgctgctgct aatgggcttt cttctggttc ttctgcttgt gcatattttt
    17161 tcagcaagcg tcgttcttca tgtttggtca ttttcttctt ctgctctttt ttatcaagca
    17221 atttttctgt cgttccatcg tatttacaac ggaaataagc accgttttta ccttcgatga
    17281 tttccatttt ccggtgacat tgcgggcatc gatgattaga gacttttgga tcttttcttc
    17341 tgcggtagtt gcaatctgaa ttggtgcaga catagatttt gccgtcacgt gtgtttttct
    17401 cgcgcagatt ggccccacat tctgggcatt tcttttgggt aatcgagaaa tcttgatatt
    17461 cttgtttgct tgatttgatc tcattcacca agcgttttgt ttctttttcg attccattca
    17521 agaaggttcc tgcttgtttc tttcctgcag caatagcttc aaggtctttt tcccagcttt
    17581 ctgtcaaatc tggtgttact aaagagggat ttaccaatac taataattgc ttacctttag
    17641 gagatacttg cagtccttga ggggtacgtt ccatcagttc agatttgatt agtttctcga
    17701 taatctctgc acgcgtagct ggggttccca gactgtattt ttccatttga ccaagcaagc
    17761 ttgcttctga caatggttta ggcggtgcag atagttcttt tttgatggcg aaatcgggat
    17821 tgattctcat tcctttttcc catttgactg tttccgtctc agtttcagtt tcgcctttcc
    17881 atcctgcttg gacgacacga ttttgccgaa aaatgaatcg ttctttacca aattcaaccg
    17941 taacttttgt ttggctgact ttatggggtt gagcaaacag tcctaaaaaa cgagagacaa
    18001 tcatttgata gatttttcgc tcttcattac ttaatttttc ataacgtgct ctgttttcag
    18061 ttggcaaaag tgcatggtga tcggtcactt tactgtcttg gaatactttt gtctgttgga
    18121 cgatggctcc gtttttcaaa tactgcttga cctcaggagc aaagtcgctg actgcttgca
    18181 atcgttcttt catcgtactt ttcatgtcat ttgtcagata tttgctgtct gtacgcggat
    18241 aagtaacgat cttatgggtc tcatacaaac tttgtacaag acttaatgtt ttctttgcag
    18301 aatagccata gcgttgattg gcttctcttt ggatctctgt taaatcataa ggcaatggcg
    18361 cagattccgt ttttaatttt tcttggactt ctgtgacagt tcctttgctt gtactgatca
    18421 attgaacaag tttttgtgct tcttcttgtg tttttaaagc aaattgattt ttttgaagca
    18481 ttttggcttg ttcttttcca gcttgcaacg tgatcgtgaa atacgtttgt ggacggaact
    18541 gctcgatttt cttttcttgg tctctcacta atgctagtgt cggtgtctgc acacggcctg
    18601 ctgataaatt atccgcgtac ttcacagtta aggctctggt cacattcaat cctaccagcc
    18661 agtccgcttt tgcccgggca agagcagatt catacaatgc atcatattct ttcgcaggtc
    18721 ttagttgctt gaatccagtt ttgattgctt tatctgtttg agaagagatc cataaccgct
    18781 tcactggttt attgaaatgc acatactcta agatccagcg agcaacaagt tccccttctc
    18841 ttccggcatc tgtcgcaatg actgcttctg agacatcttt tcgctgagcc agctgcttga
    18901 ttgcttttaa ttgatgtcct gttttaggaa gtggtttgat tcctagtttt ttagggatca
    18961 ttggcaacgt gtccatttgc cagctctgcc attccttatt cagatcttcc ggcattttca
    19021 atcctaataa atgaccaagg gcccaagtaa cgatcacctt gggtccttca taataatttt
    19081 tgtgcttttg attagcacca agcaccttgc ttaggtcttt cgcaacgctt ggtttttccg
    19141 ctagaattag ttgtttcata aaattccttt ctgctttcta ctgtgatggg ctgatgattt
    19201 ttcaacaacg caagtccctt gtattgctgt aaatcatcgt cacactcttc acaccagcgc
    19261 tcatctccgt cattccaaaa ggcgctgaga aagttacttt ttgcattgat atattcataa
    19321 ttttgatgga tttcgttcca ttgtttttcg tacgcttgtc gtgcttcttc gaaagactcg
    19381 aatgtttctt cttcttcaat atcttcttcc cagtcttcaa aaaaccacca aggctcattg
    19441 tccccgaaca tggtaattac ttgatacata ctcgtgctcc ttctttcagc ttttctagac
    19501 tcatcttagt cacttttcaa gaaagttgca agaattttct cttataaaag ggtcatttaa
    19561 tgaacatctt aaaattgtta caaatgaatc tgattgttta aggcaaagta gcgataaagt
    19621 tattgatcat ccaaaaatag ctgtcttctg gatcgattgt tggttctttt gtaaagtatc
    19681 ctaaaaaacg taaggaaaga taaccgtgga tcatactacg aagttccctg cttcgatgaa
    19741 ctttttcttc tttcgttaat tgaaaagcct caaatatctg caaaatgatc tgattcgttt
    19801 catggattcc atctaataaa acagcttcat tggtatgcgg tacactgatc agtaactcat
    19861 acactgcttg atttgaaaaa gcaaaggatc gataagtttc tgcatagatc tgcagtgctt
    19921 cctgaccact ttttcccaca agagcccgtc gcagctggtc attaagttcc tgcagaagtt
    19981 tcgctgttag cgctgttttg acatcacgaa tatttttgaa atggttatac agcgagggag
    20041 ccttgatatc caagcgtttg gctaaatgct gaaaggttat ttgctgaaca tccatctctt
    20101 ctgctagttc tcgaaagcag tcgatgattt tctcttgaga cagatttctt tttttcattt
    20161 ccatgtttcc tctcataaaa taaatccttc actctttcag tttaccacga aaaagaaaga
    20221 gaaaaaagag actttcattg ataaactaca tattgtagtt tataataaag agctgtagat
    20281 gaaaattttc agaaagaagg agtctaattg gctgcaagaa taaaggaaaa attatttagg
    20341 aaaagagaaa cttgggaaca aaacttgttt gtcttatggt ttggaacatt tatggctggt
    20401 atcggattta gtttagtcat gccttttatg tcactttaca tagatacgtt aggtaattat
    20461 agtacgtcac aattgaactt ttggagcgga ttgacttttt catctacttt cttggtgact
    20521 acgcttatct ctccttggtg gggaaaatta gccgaccaaa aaggaagaaa attgatgctg
    20581 ttgcgtgctt cattaggaat ggcaatcgtc atttctttga tgggttgcgt gactagtgtg
    20641 tatcaattag tcgcattgcg tctgcttcaa gggatttttt ctggttatat cagtaatgcc
    20701 actgcccttg tcgcaactgg gacgccaaaa gaaaaaagtg gtcaagttct tggaacctta
    20761 gcaactggtt ctgttacagg aacattactt ggaccgcttc ttggcgggat ctctgcttca
    20821 gcgtttggct atcgccctac ctttttcatt acagggatca tcttattttt cgtcttcttg
    20881 ttgagtctgt ttttcgtcca tgaaaaattt gtcccagtga aaaaagaaaa catggtttct
    20941 gccaaacaga ttttcaaaga cttgaaatat ccacacgtcg tcatcggtat gttcgtcaca
    21001 acgatgatca tccaagcatc caacaattcc attagtccaa tcatcagtct gtacattcgc
    21061 caattgctgc atggacatgg gaatgttacg ttgatcagcg gaatcattgc ttcgatacct
    21121 gggattgcca ctttgatagc cgctccaaga tttggtcgct taggtgacaa gattgggagt
    21181 gaaagaatac ttgcgattgg tttgggtttt gcgattctgg tttatatccc aatggcattc
    21241 gtcacgaatg tttggcaatt agctgcctta cgttttttga ttggtatctc tgatgcttgt
    21301 ctgctccctg ctgtccaagc attgatcaca aaatattcgc ctcatcaagc tgctggacgg
    21361 atcttcagtt ataaccaatc tttccaagca actggtaacg tagtagggcc tatgatcggc
    21421 tccagtgttt ccagtgcatt tggttatcgc ggcgtctttc tttcgacttc tcttcttgtt
    21481 ttggtcaact tcttgcttgt acgacacaat acacaagaga tccatcagca agaaactgca
    21541 gaagaaaaag cttcaggagt ctccatagct tcagggcact ctccttctca tagacaataa
    21601 gcgtaaatga aaaacactgc ttgaacagtt gtcacagact gtccttgcag tgtttttgtt
    21661 taggcctgtt ttttcaagaa ctcagataag aggaataatc ccttttccaa ggtttctttt
    21721 ttacaacagt aacctagtcg ggcatgcttc ggtaaatcaa aggcacttcc tggtactaat
    21781 agtactcctg tttcttctaa taaattgata caaaatgtct ggtcatcttc ttcgatggct
    21841 aatttaatga aagaagtaga gacgtaattg ggagcgacaa gttcaacttt cggttcattg
    21901 gctacccatt gttttaaaat cacaaggttc tctgtaatga tctttttgtt tcgcgccaat
    21961 attttttctt tattcttcaa tgcatgtaca gcaagatcat ctgacaggac tccgccgcaa
    22021 atcatcgtgt agtctcgata ctttcgaaaa acttctgcca actcaggtcc agtagctgtc
    22081 cagccaatac gaatacctgg aatggagtag gttttggata gggaattggt tgctatacct
    22141 ttttcataga catctacgat cgacaagaat tcacctttat ccgttaaagg cgcatatact
    22201 tcatctatca atacatatgc atctactgta cgagcaattt ctgcgatttt ttctaaagtt
    22261 ttccgatcta gcaatgtgcc tgtaggattg tttgcactat tcaaacagat gatttttgtt
    22321 tcaggtttga ctaaggcttc tagctgttct aaatcaaact gccaatcttc ttcttccttc
    22381 aaatgaacaa agtcaacagc ggcacctaaa gatttgggga tgtcatataa ttgctgataa
    22441 gtaggaagca tcgaaacaac atggtctcct gcttccacta acgcgtatag agctaaaaga
    22501 ttagcaccag tcgctccatt cgtttgtaag atgttttccg gatcgacggt ttgataaaga
    22561 gaagcgactt cttgtttgaa aacatctgat ccttcgatcc agccatagtc caatggttcg
    22621 tttttgcgtt tttcgaaaaa tttacttacg ctagtcccat ctaagccaac gagttcgtct
    22681 aatgtcaatg cttcgatcga gctttgtgca atatcatata ccgctttctt ttcccattta
    22741 tttagccatt cttctacacc gaaatttgcg attttcatgt ttctttcccc cttatatctt
    22801 gatccagcct acagcaagca acccgattga aagcagtgcg gccacacaaa tcagcccaat
    22861 cattactttt tcttttcccg taatttgatg tttggtttca cgacaagcga tccagtagaa
    22921 gcaaaaacca ggaagatagg caatactaca taaaaggaca taagaaaatc ctgctaaaat
    22981 gatgcccatc aattcaaaaa ctacagcaat cccaccgatc actacttgtt ttttgattcc
    23041 ttcttttaaa gaaaactgga cttgatagat cccgacaagt agataacaaa tcaaaattgc
    23101 cgctgtacat aaagaatagg caaattgata ggcataatca gtaactaaga aagtcaataa
    23161 gaatagatta gtcaagattg cagtaacaac taacgaataa gttggggcat tttttttatt
    23221 cgtttttccc caacctttcg gcaacagacc gtctcgagcc atcaacatcg ttgtttctgc
    23281 tgggagcatc gtccaagaca accaagaacc aatgatcgaa ataatcaagc cgatattgat
    23341 cagcgctgct ccccaatttc ccacgattcc ttttaaaata tttgccatag caggttgtga
    23401 aatagttgct aactcttctt gagataagac gccgtatggc aagatcgaag caaggatata
    23461 gataaacaat aaacaaagca gaccaatgat cgatgcttgc tgggcttctg aacgtttctt
    23521 cgcgcggttg gctaaaacac ttgcgccttc gatccctaca aagacccaca tcataaccat
    23581 caaacaacct ttaatctgca accagatcgt tccttgctgc cctgttccat tcactagatt
    23641 atcagatacg ttaccccaaa aatcagcagt gaatatattc actttaaatg cgataaatac
    23701 aaatattaaa aataggaata atggcactaa tttacagatc gtcacgattg tattgatgac
    23761 gctcgctcct tcaactccat tgtttactaa taaagtcaga ccccaaataa aaatacttgc
    23821 aaataaaata gaaggaacat tttgtccacc tttgaatatc gggaaaaatt ctcccatggc
    23881 gctcatcagc atcgttgcaa aagcaacatt tcccagccac gcagacaacc agtaacccca
    23941 accactgatg aagccaccta atttaccaaa tgaagcttcc gcgtaaccga aaataccacc
    24001 atctagatct ggacgctttt ctcctaaatg attcagcgat aataccaaag ctaaaatacc
    24061 aatcccaacg attatccatg aaaggatcgc tggccctgga gctgcagaac tagccagatc
    24121 acttgtgatc ccaaacacac cgcttccgat tgacgaactg acaaccagag caaccagtcc
    24181 ccatttgcta atacccgtcg atttcttttc catttccatc accctcactt ataaaattga
    24241 acaaatcact tgcaaaagat tcccttctat tttgcaactg ctcttgctta acaaaaagca
    24301 actttattca tattattgga tattatgaac tttttacaca agtatttcaa gtataaaatg
    24361 aataaaaatt aattttattg caaaaaaaca gctatgctat ccaaaagaaa aggtgtttct
    24421 tttgttagcc atagctgttt tgattcattt aatatcgctt aaacgtattg taaaactttt
    24481 gtttcttgat atgcgtggtc gatcaaacca ccacccagac attccattcc atcataaaat
    24541 acaactgctt gtccaggagt gattgcacgt acaggttcgt cgaatacgac ttctgcacga
    24601 ttatcatcca ataagcgaac tgtcacagga acgtcttgct gacgataacg gaatttcgct
    24661 gtacacttga attcttttgg cattggttca ttcgtcgtaa agtggatttc acttgcatcc
    24721 aagcttgtgg catacaatgc aggatgatgg aacccttgcc ccacatataa tgtgtttgtc
    24781 gctagatctt ttcctacaac aaaccagggt tcttgcgaat ctcctccgcc accgatccct
    24841 agtccttgac gttgaccaat cgtataatac atcaagccag catgttgtcc tttgacttca
    24901 ccatcaagag tgaccatatt tccttttttt gctggcagat agttgctcaa aaattgtttg
    24961 aagttctttt caccaataaa gcagattccc gttgaatctt ttttcttagc agtcgcaagg
    25021 cctgcgcgtt cagcaattgc ccgtacttct gatttttcca tgccgcctag agggaacatc
    25081 gtttttgaaa gctgttcttg tgataattgg ctaaggaagt aagtttgatc tttattattg
    25141 tctactccac gaagcatgtg gaccacgccg ttttcatcgc gagtgacttg cgcataatgt
    25201 ccagtagcta cataatcggc gccaagatcc attgcataat ccaagaatgc tttgaatttg
    25261 atttctttgt tgcacatcac gtctgggtta ggtgtacgtc ctgcgcggta ttcggctagg
    25321 aaatattcaa atacacgatc ccaatattct ttttcaaaat tcacagagta ataaggaatc
    25381 ccgatttgag cagctacttt cgctacatcc ttataatctt ccgtggccgt gcatacaccg
    25441 ttttcgtccg tgtcatccca atttttcatg aatattccga tcacgtcgta tccttgttcc
    25501 tttaacaaaa gtgctgttac agatgagtcg acaccgccgc tcatccctac gacaacgcgt
    25561 gttttgctgt tgtctgtcat gatatcacca tcatttcaat tagattctga aacatctacg
    25621 agttgaaggt cgcaactttt cagtatttta cattatacgt catatttctg aaaatttcat
    25681 cccttaatta tcaagtcgta aaaatactgt tctactggac ctattcagta cacaaacatg
    25741 atagtcaacc aaaaaatcac tcatcttttg agcattcgat ataaaaaatt ggcatactca
    25801 taaaaagggc cagcctttcg gcaataagga ataattttat aaattgtgaa aaactatttt
    25861 tcaaaattgc ttcttatcgc ttaaagctgc acgtctcatt tacaaccttt tctttttaga
    25921 tgttatgagt tgctcagata agtgattttg tttactctaa gaaaaaaggg aggcaaaaga
    25981 tacatgaatt actttatcag tgatatgcac tttttccatg aacgcttatt aggaaaaaac
    26041 gactttgctc cgcggctctt tgcaaatgtg gatgaaatga accaacagtt gatcactagc
    26101 tggaatgcag taataaaaga aaccgaccat gtttatcatc taggcgacct tgctatgcat
    26161 ccgcaatatg aaaaagcaaa tgcagaaatt ttggaactgg tcactcaact gcaaggaaca
    26221 atccacttga tcaaagggaa ccatgacagt cgcgcttttt tccgttattt agaaaaaaat
    26281 gatcctaggc ttactgacgg aagtcccaaa ttttattttt atgatgtagg tgccatcgtt
    26341 aaattcgacc atcaacaata ttacttgact cattatccga tgttgctcgg gcctaacgac
    26401 actatccgca atcttcatgg acatatccat cattacagcg tacctatcga gaacgatgtc
    26461 aatgtaggcg tagacgcacc ggaaagagaa ctcttaaagg aaaaactacc atttggcact
    26521 ccgttatctg agcaacaaat caaagaaatc tatgaagcaa agaggaagta cgataggtcg
    26581 tgaaaaaact gccctatatc aagtattaaa caaataaaga taattactca tatttttcat
    26641 ttgagtaggc ttattgccac aggaacatta ggatcagaaa tggagttgta acaagacttc
    26701 tgtcacaact ccattccata ctattcgtct ttttgctcat ggtgttcttt taccagtttt
    26761 ttctgtaatt ctttatcata atttcgggca gctgacgtta aaaataaaac gattccgcat
    26821 aataaaatcc cgattccgga aaacaagaac attttttcta cccctaaacg atcagctaat
    26881 ggtcctgcaa ataataatcc aattggacct gccaggttca tcaaagagtt gaagttccct
    26941 aagacacgac ctaaaacatt cgattcataa ctctgctgga tcattgccat cagcagtgta
    27001 ttgaaatatg gcgttgcaca acctgctcct gcattcaaga taagaaagta caagaaccct
    27061 tgactatcgt ttggaaccaa tccgcttgca ccgatcgtca ggcccataat gatgtaggct
    27121 ataataatag gtttcattcg gtctttccat gttcctataa aactgatcag tgcaccgcct
    27181 aagagcattc cgactgcgta gactacttcc actgctcccg cctgtccaac ggaaccacca
    27241 aagtaatcta gcgtcatcaa tggatacaga ctgattgctg gcatgaacaa cagcatcaca
    27301 aatgcaccat tgatcgttat gtaccataac cctttattat ccatcaattg ctgcagccca
    27361 aattttgcat ctttaaaaat agtaagcgga acttccaata tttcaggaga aacttttggg
    27421 atcttcacaa aaatcaataa gccgacacca aaaactgccc ccaatacatc taataaaata
    27481 aggtaattga caggaacgac tgaaaacaaa gccgcaccta gagctggcgc aatgatgaaa
    27541 ttcgctgatt ggaccattcc taactgtcca ttcgtccttg ttatgtgaga agatggaacg
    27601 attgtcggca taatcgactg gatcgttggc atttggaatg tttgtgcgac cgaacgcata
    27661 aacaaagaca caaaaactag ccaaatcggg aaggattctg aaatcgttcc gactactgct
    27721 aaaataagcg cgaaaatcgc tacaataata tccgtgacaa tcaaaagagc ttttttatcc
    27781 cagcgatcca ctagcggtcc tacaaatggg cttagtaaaa ccatgggaat cattcctagt
    27841 aaagtagcaa aactcaaaat tgtcgccgaa ccggtttctc ttgtcaaata ccagatgatc
    27901 gcatattgga cgaccatact agtgattcct gacaaaaact gtcccgataa aaagaacaaa
    27961 taatttctct tccaattcgt taaaacgacc gactgttcat tttccataaa catccctctt
    28021 ttctttttca ttttgtatag cgtttgcaca acaatcatta tacgggattc ttttttcaaa
    28081 aaataccttt ttccctaaat aacaatcgtt tcaatttaaa tttctttact tagaaacaat
    28141 aaatcagcaa aatattaaaa ataacaacat gtgttaaatt taaaaataaa aaagacaact
    28201 aatttgcgtc gtccttacaa agttagtcat ctaacaatag gccgtttcaa aactagttgt
    28261 ctttttatat ccacagatca ttcgactttt tcgaataacc cacggctaat catcccatcc
    28321 atcagaaaat aaaatttatc ttggctcatt ttctgttttg gatctttgaa aatattgact
    28381 gctttcaatc catttgctgt agtgatcgac attttcaatg tttgaatatc ttttgataca
    28441 gtgattttta atttgaatcc ttctttgctt tgcggcgtgg catctaatgg tcggatcaat
    28501 tgataattgc ctccgtttgt ttctgtaaat ccattatcgc gcaatgtata tctttttgca
    28561 tcaggatgca aacgatagct aattggtgcg cccagtaccg ttgctgttgt ttcaaatgcc
    28621 atgattttca cctcgataaa attagtttct ctttattata aaagtttatc ctgattttta
    28681 ctaggttttt cagttaagtt ccctaaaaaa tgaaggcgtt ctctcagaca tctgttcata
    28741 taagaatgac ttcactagtt attttttgac agcacgaact aattgatcaa ggaaatactg
    28801 gatgtcgtct tcggtatttc ccaaaccaaa gctgatacgg atggattctt ttagtatcgg
    28861 tgtatcttct ccatacattg cttctaatac gtgtgaaggc tcgatatttc ctgctgtaca
    28921 agctgaacca gtagaaatag cgaccccatt aagatcaagc ttcatcagta gcaaatcatt
    28981 tgcttcgcca attaagcgaa gattcaacac atgagggagt ttattcgtta aatcaccatt
    29041 tacttgataa gggatttcgt ttgcttctaa tgtatcaaga atcaattgct ggaatccttt
    29101 atatttgttg ttacgtgctt cacgttcttc aggtgacagc agttcaactg cttttgccat
    29161 accccagatc cctgctaaat tttctgtacc tgcacgccgc ttttcttcct gctccccgcc
    29221 taaaagcaat gcagggatat ttactccgtc tcgtttatat aagaaaccga cgccttttgg
    29281 cccattgatt ttatgagcag agatacttaa taaatcgatt cctaattctt ctggatggat
    29341 cgtttggttt ccgtacgctt gtaccgcatc tgtatggaaa taagctggat gatccgctaa
    29401 aagttcaccg atttctttga ttggcatcaa atttccgatt tcattgttcc cgtacatcac
    29461 agagaccaag atcgtatctt ctcgtaacgc ttttttgacc tcttcgatcg acagattccc
    29521 tttttcatct acaggcagat atgtaacgtc aaaccccttt gtctctaaat atttcatggt
    29581 attaagaaca gcaggatgtt cgatagctgt agtgattaga tgtctgcctt ctttttgacg
    29641 cgagaacgct gtttctaaga tagctgtatt atccccttct gttcccccac tgttaaagat
    29701 gatctcgtga ggttttgctt gcagactgtc tgcaatgatt tgtcttgctg tttccaattt
    29761 ttcatgcgcg tggcgaccaa atccatgtat gcttgaagga tttccaaacg tgtcttgcat
    29821 gattgccgtc atcgcttcaa ttacttgtgg atgcatgggg gtagttgccg catgatccag
    29881 ataaatagtt ttcacttcat tcatcccttc caaattattg ctaggcacat tctagcataa
    29941 aatagcgaac aaaccaaaga agagctcatt tttgtctaga ggacgtttca tgggcataga
    30001 aaaaaggagc tgtgacaaaa gtcttgtcgc agctccttga agctgctcac ttctgtcaca
    30061 acttctttcc ctcttttttt tgtttattcg aattcttttt cttccagacg gaataacgga
    30121 ctcatcggtg tattttcatg gatgcgtttg accgcttcac ccattaattc tgaacatgta
    30181 acgattgtta agttttctgg atgacgattt tcttctgtga aaacagaatc agtgatacaa
    30241 atatctttga ttggtgcatt gtctaaaata cttttcgcat gatcggacaa taatccatgt
    30301 gacgcacagg catatacttc ttttgcgccg ttttctttta atacatttgc tgctcgtgca
    30361 agcgttgctc ctgtgttcaa gatatcatct accatgatac atgtctttcc ttgaacatca
    30421 ccgatcacat aaccaccctc agttgaagca tcatctgcat gatctacgat ggctaatgtg
    30481 ctgttcaaat attctgaaag actacgtgca cgctgaacac cgctgttttt tggcgaaaca
    30541 actacgatgt catctcccgt cagacctaat tgacggtaat aatgagcaaa caacggcatc
    30601 gtaaataaat tatctacagg aatatcaaag aatccttgta cttgaaccgt gtgcagatct
    30661 aaagtcagta ctcgagttgc ccctgcttct tcgatcaagt tagcgatcaa tttcgcagtg
    30721 atcggttcat gaggtttcgc cgtgcggtct tgcctagcat acccatagta aggcaatacg
    30781 acattgatcg ttttcgcact tgctcgcttc attgcatcga tcatgatcag tagttccata
    30841 tagtaatcat tgacaggtgc attcgttgat tgaacgatat acacatgatc ccctcgaata
    30901 ctttcttcga tattgatgga aatctcgcca tcactaaatt gcttcacgac gcttttacct
    30961 aattctgttc cgaacacttt cgcgattttt tcagctaatg gacggttgcc attcaaacta
    31021 aaaatcttca acgtgtcatc ctgatactta ttactcatgt agtcctcccc aaaacttctc
    31081 tattcttcga tagtttaaca tatttttcca agattttctg ttaatttcag caaattctta
    31141 aggaagtgaa actgttccgt tactccaact ggcaggaatg actttatgat aatcgcccca
    31201 gttgtcgtgt actttgtaat aacgttctcc tgtttcagtt tcaaaataag cgtacgctgt
    31261 cacccaatga ttgccatatg tactgccaag tactttcaaa ataccaatca tcacaggttt
    31321 gccttcacgt atacgttttg tagctcgctg ccatgaacct agaagagttg ctcgtgcacg
    31381 ctcatgattg ccattcttct ttaaaaaact agaaatccct gtactaacct gaaaaggaat
    31441 cgtaggaagt cctagtggtt ggatctggct acgcaatgcc ttcgtcaaaa ccaacgaatc
    31501 agccgtattt ttcttgcgaa tctcatttgg aagcatccac cccttacgat agtcttgata
    31561 ataggcaagg agaactgctg ccgcgtaagt accacaaaga tagcctgagg gggtagccca
    31621 tttcttatac ctcaaaaaac gttcttctgg caatcccacc cagcaatttg cgagttcctt
    31681 tcctgacctt ttttcttcga tcaaaacggg aatatcttct tttctgttga tccatctaga
    31741 taactcgatt ggctcttcat tgatcagctt ccatttttta caagaaagac cctcttgata
    31801 agtatatttt gctggagaat attctaccgg atgtcctgat tgatcgataa tcacataact
    31861 ttgatcttca aaaaccagag ctaaaaaaag caatttactt tttcttgact gcagctgctg
    31921 agtgctggct aattttttaa tttttttatc tactttgaaa tataaaggtt tatccactac
    31981 ttgattcagt tgggatcgcc tccttttttt cattataagt gaaggattga aaaaaacaaa
    32041 tcagtttgat tgttcgaatt ttcaattttt tatgttctat ttgttaattc cttcattttg
    32101 taagataatg ggcacacgaa acaaaaaagg agcgatcgat gaatgaacaa agaacgattg
    32161 aaacaagatt tctatgaagc agtcaatgaa gaatggcaaa aaacagcaaa aatccctgca
    32221 gataaaccag ctacaggcgg tttccaagat ttagttgacg ggatcgacca acttttgatg
    32281 ggagaaacag acgatctgct tgcccatcca gaaaaagtgt cttcagaaaa aatgaaacat
    32341 ttccttgcat attatcaact ggcaaacgat tatgaccgaa gaaatgagtt gcatgctcaa
    32401 ccgcttcaac ctataatcga aaaagttgaa tcattgcagt ctttcgacga attgaataag
    32461 caatttccag aatggctgtt ggatacatta ccattgccat ttaatctgga tgtagatgca
    32521 gatatgaaga atgcgcaaac gaacgccttc tttgcttatc cgccttcttt attccttcca
    32581 gataagacgt attatgcatc agaccatccc aacggtgcac aactaatagg cgttttcttt
    32641 gatatggctg ttcagttatt agagcttgcc ggcaaagaaa aaagtcaggc agaagcaatc
    32701 acggaacaag cagtgcaatt tgataaattg attgctcctc atgtaaaaag tgcagaagaa
    32761 aacgcagatt acagcaaaat gtataatccg caatcttttg atacatttat taaatacaca
    32821 gataaactgg acttgaaaaa acttgtcacg ggtttgattg ggacaactcc agataaagtg
    32881 atcgtaactg atccagtgta cttcgatcat ttgaatgaca tcctgacaga agctcatttt
    32941 ccgttattca aaagctggat gatcattaaa acagtacgtt ctcttagtgg ttatctatca
    33001 gaagaattcc gccaagtcag cggaatctat tctcgcgtat tgtctggaac agacgaagca
    33061 atgcctcaga aaaaagcagc gtattacctt gcctctggac aattcgatca agtagttggc
    33121 gattactacg gaaaaaaata ttttggcgaa gctgctaaaa aagacgtaga acaaatggtg
    33181 aaaaaaatga tcgccgttta taaaaaacgt ctagaagaaa atgactggct tggggatgca
    33241 acaaaagaaa aagccgtcat aaaattaaat aacttaggta tccaagtagg ctaccccgat
    33301 aagatcccag cattattcga ccagttcaaa acagtacctg cagatcaggg tggtactttg
    33361 ctttcaaata ctcttgtttt cagcaaactc acattaaaag atcgtttcag caaatggaac
    33421 aaaccagtgg atcgttcaga atgggaaatg agtgctgaca cggtcaatgc ttattatcat
    33481 ccatttcgga atgtgatcgt ctttccggca gcgatccttc aagcaccatt ttacagtcta
    33541 gaacaatcaa gctctgctaa ttatggtgga atcggagctg ttatcgctca tgagatttcc
    33601 catgcatttg ataataacgg cgctttattt gacgaatatg gcaacttgaa caactggtgg
    33661 acagaagaag atcttacaca atttcaaaac aaagcacaag caatgatcga agaattcgat
    33721 ggtctgcctt ttgctgacgg aaaagtaaac ggaaaattaa ccgtttcgga aaatattgct
    33781 gatgcaggcg gtctaagctg tgcattggaa gcagcaaaaa cggaatcaga tctttctatc
    33841 gaggaattct ttgtcaattg ggcaacgatt tggcgtacaa aagcaaaaaa agaataccaa
    33901 caacttttac tgcaaatcga tgtccatgcc cctgctaaac tccgagcaaa cgtgcaacca
    33961 aaaaaccttg acgaattcta tgaaacattt ggtatcacgg aacaagacgc tatgtatcta
    34021 gcgccagaaa aaagagtgcg aatctggtaa gaggttgtgc cagaagtggt cagcttcaag
    34081 aaataagaag gaatttggga aaatcgcttc tcaaattttt gtgacatttc ggcttatttt
    34141 tcgaagaagt tacttctgga ataccatgta ttagaggttg tgaaaaagct gcttagtttc
    34201 aagtagtaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttgacttac
    34261 taccgagaaa ctggcttttg aacaccgttt atcgagaggt tgtgaaatca gcgtcctgac
    34321 ctgagtatta gagaaaaaaa cggaaaaata gttcctcata ttttgccgtt tgttgagcta
    34381 ataccgaggg tcagctgatt gaacaccgtt tagtggagag attgtgaaaa agctgtcctg
    34441 catcaagtaa taagaacagc caatcaaaag cagcaaggag aacctcatct ataaaaagat
    34501 agaaggaagt gagacatcta ttggtctcat ttccttctat ttttactttc ctcagtcaat
    34561 tgttttacat cataaaagga gaaattccac ttattttttt cgctgtttca tgacataaac
    34621 aactacaccg attaacaaaa ttcctccagc taatgctagc aacgggttat taaattctcc
    34681 cgtttttggg agtccttgag tgccattttt ttcactttct tttgctgtcc tatccggatg
    34741 agaaggtttt ccatttggtt ttccatttgg tgattcattt cctggatttt ctctttctgg
    34801 tttgtcattt cccgggggca catcggcagt ttctcgcaca gtgatcgctt tttgctgggt
    34861 aacagattct gtatgagcag accatttttc cccatcaaat atttccgaac gataagtgac
    34921 ttctagcgta tattctcctt ttgaaggtgc tgaaaaaata gcggtatgat tagaaaattg
    34981 agcacttgac tgtgtttcaa cgatttggat cttttctggg aaataacgca tatcttcaaa
    35041 gacaggcgtt ccttgatttg gtgtgaaagt tgacacaaaa actgtaaaag gcttcagttc
    35101 aatgatattt ccttctgcat tctccgccac accatttgct tcttgtataa taatcggatt
    35161 attttgttca tttggttttg tctgatttgg ttcttgtacg attgcatact ctggatattc
    35221 cgaagacggt gaggtttctc cgtcaaaaat agcacctaca gataatggaa gaccatccac
    35281 tctttccttt tctgtttgac gattccccgg tttaatctgc atcagtccat gaacatggat
    35341 acttccattt tcatagcgcg taacaggctg ctgaccttct ttcgaacatg tatttagaga
    35401 ctcgatccaa tcttttgata tggtatgtcc aagagtattt tctgattgtt tctctcgttc
    35461 attccaatag tagttcaacg agtggtagat ttgatcttcg ttttgattca atgggcggat
    35521 ctgctctctc atctcaagat tctctgttcg gaaagataag ataccagaag catggtagtc
    35581 tgtgtttcct gctgaagagg tataaagagc cacattatat gaaccgttat taaaacttgt
    35641 actgctattg acgataatat ctgttccgct attggaatcg attcctttcg caagattttc
    35701 gaaagacaaa ctattgatca attcatgagg gcccgttaaa gaagaaccgc ccattttgaa
    35761 tccattaccg ttaccttctc cctcaattcc tggtacccag ccgtttcgat aagcaactga
    35821 gtttttgatg attacttttc cgatcgaacc tgtttcattt ttagcaaaaa ggtcccaacc
    35881 gtcatctgca ttatgatacg cgatacaacc gtcaaataca tttccttctc ctattgtcag
    35941 tttggccgca aatccatcgg catcttcaaa tcctggatcg gcattttcaa aagatgtaca
    36001 gttcttgact aagttatgcg cgggccatgc ttcgaaagga tcatttgagc taccgctgat
    36061 ttggattcct gtatttccat tccgataagc atgaatctct tcgatcaaat tatgatttcc
    36121 agaaatctgt aatccttttt gcatagctgc actatttgta atgtcaaacc ctcgtaaacc
    36181 ccagtagtga ctgaacagtg tcatcccatt tccagttcct ttaaaatcaa gtacaggacg
    36241 cttgttttct ggatttttct ctgcaatcaa ctgaatcggg ttttctcttg tcccgttaac
    36301 gccttttgga atcgtcaatc ctctttcgaa ggaataagtt ccaggcgcca tgacgattgt
    36361 ctgcccagca tatgcgtatc tcaatgcttg tgtcagagat aatggatttt gtctgcttcc
    36421 gtttcctgca tctgaccctt ctggtgttac ataaactgtc gaatatttag gtttacggta
    36481 gtccactgta tgaagaatct cttttgtttc ataactagtc atctctacgt attcttctgg
    36541 cggatagttt ttatctggcg taaaactgaa agtaaattcg ttcgttcctt ccatcagttt
    36601 tgtctgataa tcaaattccc gattcgcagt gatagccaag ttttttgcgt tcttaagcac
    36661 ctctcctgtt gctttatttt taattgtcaa taatccgtca gcatttgctc taaaggacat
    36721 agtatgatct tggtttcctg aatgattact agaggtcaca gtgtaaatcg gatcaatcaa
    36781 gcgagtaggt ctttcttgcc caggaggatc tgtcgcagga tctgagtaag caacttcgat
    36841 tccttctacc ttgatcgtca tattccttgc tgcagcaaat cctgcataaa taaccgattc
    36901 atctgaagcg tataattttt cccagtcata catgatgtca gaagccattt cttctcctgt
    36961 ttcaggatcg taataatatg cctctaaacc agtattcgtt tttttcatct ctacgtccag
    37021 tttagtaatc gagttcgtgg ccggaaacag attcccatga ccgtttccga atatattata
    37081 agaacctgtt tctttttcca agaaccctgc tgaagtctct aaaggtgttg tttccactga
    37141 gacactccca ggagctggcg cagcttgcgg atcttcagat gatattcctg tgattgcccg
    37201 tgtccccaat ccaagtcgca tcgtatattt gctgccgctc gtgttgacca cttcttgtct
    37261 atccgagtcc cagacatatt cgatccgact gcctagtatg gcaaatgaat ttgaataaaa
    37321 tgcctgccca aacaaatgat tttttggtac tgcgtctctt accattaaag caaatccttc
    37381 ttgggcattc gtataggtcc aagattctat ttcgatcgtc gcccgcatcc taaagttgat
    37441 gtctttgggg attgcttgat aataataggt cattccatct gatccgcttg actggaattt
    37501 tccgccgtta ttcgtggaag agagccgcac gccgtcttct aatggttcaa tggcatacct
    37561 actagcactt gtgctttctc cgaaaacaac ccctttccac tcttcatttg cttcttcaga
    37621 ttgctttttt gtcttttcat tttctgattc tttcatcata ggatcatttc cttcttccgt
    37681 atccatatct tcactagttc ttggtatttc ctccattgtt gacatccctt caatgttcac
    37741 gtgtgcattt tcttcgttca cagactgttg ttcgttagca gcaacttcaa cgacagaaat
    37801 taaaaccggg gtactgagca taaggagatt catcattagc gcactgtatt ttatacgttt
    37861 ttttaacaag tgtattcctc ccgtaacaaa ttagaaagcg tttacttaaa gtattaccta
    37921 atttattttt actgtcaata aaaaacaaaa ttataataag tgtattttat aaaaagaaaa
    37981 aatataactt aagttgttga taataaagag atttattaac ggaaaaagaa taagtttttt
    38041 taaaataacg tataaaaaag agcataaaac aaagtcgttt gttttatgct cttcttttat
    38101 tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt ctgcgcctag
    38161 tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacgga
    38221 atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac
    38281 acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg
    38341 gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt
    38401 ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc
    38461 cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg
    38521 atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg
    38581 agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc
    38641 tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat
    38701 acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata
    38761 aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc
    38821 aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa
    38881 gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat
    38941 gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta
    39001 accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt
    39061 cctgggttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata
    39121 acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc
    39181 atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct
    39241 ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg
    39301 cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct
    39361 tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg
    39421 attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa
    39481 gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc
    39541 tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt
    39601 tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt
    39661 tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc
    39721 aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc
    39781 atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct
    39841 tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca
    39901 tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag
    39961 ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga
    40021 tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg
    40081 acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc aggatgaaat
    40141 accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca
    40201 ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg
    40261 ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat
    40321 ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc tctaaatata
    40381 gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa taagtatgta
    40441 atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat
    40501 aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa
    40561 tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact
    40621 ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact
    40681 tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac
    40741 aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa
    40801 acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga
    40861 attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc
    40921 gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa
    40981 acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat
    41041 cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc
    41101 tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact
    41161 cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt
    41221 tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt
    41281 tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga
    41341 tttattgaac aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga
    41401 gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga
    41461 acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt
    41521 catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa
    41581 tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg
    41641 tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt
    41701 cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc
    41761 acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa
    41821 tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta
    41881 tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat
    41941 gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca
    42001 attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat
    42061 ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat
    42121 ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac
    42181 cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga
    42241 cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa
    42301 tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac
    42361 aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt
    42421 agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat
    42481 cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg
    42541 cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt catcttgctg
    42601 tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg
    42661 atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat ggagacgatt
    42721 ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt
    42781 ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt
    42841 agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg
    42901 gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga
    42961 agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc
    43021 gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt
    43081 cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg
    43141 attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca
    43201 atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg
    43261 gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa
    43321 ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata
    43381 aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc
    43441 ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca
    43501 atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct
    43561 aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct
    43621 agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca
    43681 acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa
    43741 cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga
    43801 ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca
    43861 ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga
    43921 ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg
    43981 gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa aatgactaaa
    44041 aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa
    44101 atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa
    44161 ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa taatccacag
    44221 atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg
    44281 cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag
    44341 ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc
    44401 ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca
    44461 gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc
    44521 gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa
    44581 ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca
    44641 ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg
    44701 attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt
    44761 cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc
    44821 catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac
    44881 atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac
    44941 gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt
    45001 cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt
    45061 tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt
    45121 aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt
    45181 aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc
    45241 cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc
    45301 atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa
    45361 ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat
    45421 gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc
    45481 atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc
    45541 gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga
    45601 gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg
    45661 ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc
    45721 actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct
    45781 tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc
    45841 taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat
    45901 cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt
    45961 aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt
    46021 gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg
    46081 actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt
    46141 tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt
    46201 ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta
    46261 atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt
    46321 aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct
    46381 gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg
    46441 atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga
    46501 agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga
    46561 ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat
    46621 tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc
    46681 tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa
    46741 aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct
    46801 cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt
    46861 ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc
    46921 actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt
    46981 tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg
    47041 tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta
    47101 attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg
    47161 agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc
    47221 gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct
    47281 cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca
    47341 ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc
    47401 atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa
    47461 ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc
    47521 ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt
    47581 tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga
    47641 tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga
    47701 ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt
    47761 aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac
    47821 ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc
    47881 gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat
    47941 attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata
    48001 gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc
    48061 ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa
    48121 gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct
    48181 atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc
    48241 agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc
    48301 atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc
    48361 ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc
    48421 atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg
    48481 tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat
    48541 cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat
    48601 cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt
    48661 atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa
    48721 ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata
    48781 agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc
    48841 ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc
    48901 tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat
    48961 catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata
    49021 agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac
    49081 ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg
    49141 atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga
    49201 attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac
    49261 ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt
    49321 agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa
    49381 tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttgacta gttttccgtt
    49441 cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat
    49501 ttttttattc atttttttct ccccgttctt tttttattaa taaaacagca cctaacaata
    49561 aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg aatacggacc
    49621 attttcctga ttgacctgga ttgcttggac cactcggctc tttcggtttg tccgttgttt
    49681 cttttacggt ctcgttataa acacgtggtg ttgcggattc ataggcagag actggaactt
    49741 tcccggattc taattcagcc atctcctgtc cgttaactac tacatattgt ggttggcctt
    49801 ttgcatccac ccaaaattca gggatgatga cctcgattct cttacttgct tcatccatct
    49861 cgtaaccttc cacacctttt aattcttcaa aataataggt gccagaaggc aagaaacgtt
    49921 gtcctgtcgt aacaaggccc tctttatccg aaatgaattt gcttacattt ttgtcattca
    49981 aaggatcatt gtctgctggt ttgatccatt gatttttcaa gtcattagca ggagccatat
    50041 ctaaataata tttcccccct tctatcatct gatatagagc aaaaacagca ccttctaatg
    50101 gttttccttt ttcagttgtt cctctttgac gtccatactt gaagaagtat ggatctctta
    50161 gataaccgac atttttagga tagatatgaa tctcttgcag ttcctcggtt gattctgtcg
    50221 ggtgataaat tggcaaaata gcagcaattg ggatagctgt ctgatcgata tcaacatcca
    50281 atccaacttc tggatcaatc gattcttcga aaatcaaata aactgcatcc cgatcttggt
    50341 ttttcttagg tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc
    50401 cagttgttac cgaagtccct atttggacaa gcctttcttc tgtgattagc tgacggatat
    50461 tgtttctgtt cgtttcagcg atttgctgga cgaattcttt aggagtcatg ttttcagcag
    50521 cttttttttc atgataataa tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg
    50581 gcaccgtctg tgaaatgata tctgctgttt cccctgcttc ctcagagata gccaagccat
    50641 cattttcatg gtattcaaat tgatcgttat aatcgatatc tcgaatcatc cgtttatgca
    50701 acacgaattc cacgacatct tcccctgatt cttctgccga catcgcatgt ggaagaagga
    50761 ctgctgcaag cggtagtaaa acaagtaatt gaagcatcaa acacaacagc tttttcatta
    50821 ccttgctccc tcctttttag attgtctgac aaacaaacca taggctgctc ctgcaagcgc
    50881 catgaagaat gcagcaagca ggatatagaa taatcgtccg gatcctcctg tagcaggcaa
    50941 cggattttca ggatcgttcc cgacactaag ctgtaatctg attcgatttg tctccaaaag
    51001 ttcatattct aactcttgct gtagatcagg cgtctctgct tcttcgtttg gataatacga
    51061 aatctcaact tttagtgtac ccgcttcttt tcctggaact acccgatcat tttctatgtc
    51121 aggttcttct cgataactaa tttctaaaat aaagtaacct ggaagttttc tatagctgtc
    51181 aggtgacttc acttccttga tccgatagac cccttccaac aagtctgaag cattgaatag
    51241 ggaagaatcg tttggatctg gtgccaagcc tccctcgatg atttcatact ctcctgcact
    51301 attttgtttc tcaattgaaa attctgcgcc ttcaagacgt cggctattca tactgtcaat
    51361 tttcaaaagt tccaaatcga ttggcgctag ttcatcttct aaggttactt gtagtacacc
    51421 atcgatgatt tcatagcctt ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc
    51481 aatgataaac ttaccttctt cattcagtgc aaactggaac acttgatcag atagccgata
    51541 cccatttggt gcgacagatt ccgagacctc atattcacca atagacagat gcagaggttg
    51601 gtcgtaattt aaatcgacat acttttctac tcctacggca cgagaataga agtatccgat
    51661 cccaggcaaa cgtttcgatt cttctggttc taacggatta ccagtgtatg ctcgatacag
    51721 cgggaaggaa gaatccccca ctttcctgat atcgaaacct gcaccatcta atgtatggcc
    51781 ttcatggtct aattttctaa ctcggatctc ccgataatca aacgtattgt aagcagtgaa
    51841 tgctacccat aaacgatcct cttcatctgt tgtatacgtg agatattgcc aattttcttc
    51901 acctgtttgc ttgagttgga tagtccctat agcattatcg taaagtcgca agatccatgt
    51961 gtcattttca tcaggttctt tgaaaccttc tgggttctgg ctctcaatga ttttgtattc
    52021 tgcatagttg gtagtttctt cactatcctt ataaacaaat gaatctggct ctccgattgg
    52081 atgaataact ttctcaccat ccacgattac atatttcccg tcttcatcta ctgaatagaa
    52141 cgtgagtctc cctttctctt cctctacttc gttcacacca gaaagccctt tcgctactgg
    52201 atatccgatt tccagtccgt taccatctgt ttcataaagt tcaaactctg cgccattgat
    52261 tggcatatta ctgccttcaa ttagtttatt gatattcatg tggattggct tgaattcatt
    52321 tcgcatttcc agtttgacat acaactcatc atactctgtg ccttctatgt taggctcttg
    52381 tacaacagaa agcttgtgat attctccttc ataataggaa ttcggatctt taccttccgg
    52441 tccgccgact ctcgaaccag atgtatattc atctacaagc tcgtagtgac ctaacatctc
    52501 atcttcgttg gctgcatatt ttttgatttg gaattgatac gtctcatcta ctaactgata
    52561 acgtggtggt ccagcgatct ctctgatcca aaatatgtcg ctgactgctc cagtttcatc
    52621 tcctaaaatc ggcatcttcg tttcaaagat cgctattccg tccgtggtag ttttacttgt
    52681 ttcgattggt tcggcttccc cactctctgg attttggtca tataatgcaa attcgactcc
    52741 agccaacggc tcgccatttt gacccttctt gctgacttgg aaattacggc ctttatgttc
    52801 gtaacgaatg gaagaagatg ggaaatacat ccgtccagta gtgatatcat tctcataatc
    52861 ctccaggttc tgaatatagg ccatattgtt cactgtgcaa aaatttccgt cttgagctcc
    52921 cgcatcaagt tttacttggt acttgatcct gaccatttcc ccttcaccta aagaaatcgg
    52981 agaaaattta taagcttggt tttcccgtcc gacatacgtt gtcggagtcg ccactacttc
    53041 tggtggggct tgtccatgac gttcttcatc ccatatttct ggctcttggt acttcatgcc
    53101 gttttctcct ggatctagat ggtaaaactg aatatctgcc gatctgccag ctccaaatgg
    53161 tgagacatta aaaccagttt catcataaag tgtggcttgc tggatcgttc tcttgaaggt
    53221 agaagagata tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg
    53281 cggtgttacg ccatcttctt ctaaatcagc aatatttttc aatacatttc gtccaatagc
    53341 atctagtcgt tgtctcccgc taacgtttcc atcgattcct aaaccaatgg agaagatact
    53401 tggcgggttc tcttgatact tgtttttcag ccaatgatga taagcaactg tgttgatcgc
    53461 agatgacttt tcttgcgaag taggactgcc attcgtattg ttattagaag aaccatcacc
    53521 aacccagtga gtcgctttat tatctccttc ttttacctct ccataacgat agtgtacatt
    53581 tacttcatct tctggacggt taaagtcaga tgcataggta actggatgag taaaccctgc
    53641 attagaagta ttcccagaaa actcctcaaa atttctgaac aaaacgtcat tatcttcagc
    53701 aatcttatct tccatcacag tccagttgct ccaagctcca ttagtgctcg tacgataact
    53761 tgactgaatc ggtgcataac tgaaggtcgg tgtcccatct cccacaacga ttaggatttt
    53821 ttctggattt ctattttctc cgccattgtc tttatacaat gtttcatagc catttttaag
    53881 ccctagtgta agcggagttc ctccgattgg tgtacgattt aatgctgtat tctcacttcc
    53941 cgtcaaacga ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt
    54001 attgatagct gtactattat ggtaaaaatt caccataccg attcgaatat ccagactcgg
    54061 gtcagagagc agatctttac tcatttcgtt tactgcctca atcaacgcag catttttagt
    54121 ttgactattg gtcccagcgg ttaattcact catactcgct gacttatcta gaacaaaaac
    54181 gatatcgatt ggactgattt cctttttctc gcccccgatg acatctagat agacattgta
    54241 aagggttgtt gcatcttcag gatgtagatc agatgctgtg ctgcttggca gaacaggttc
    54301 tacccatttt ttgatgtacg ccccgttata atccaagtaa ctgttttcaa aattccgttc
    54361 atttccccaa agctgaacgg tttgagctgg aacgtttcta tcttcttcgc ctgcagttga
    54421 tccattatta gtagcatagt cgtaatttcg ccagttcgct cccccgtttg tataatcact
    54481 atcatcgatc aagttctctc cgtcatatgg cgagcctttg tctccttgat agcgttcatt
    54541 gctttgtgtc caatttccta acacttttgt atagtcattc gttgtaaact tcgggtagcg
    54601 atgctgttcg ttttcaactt ctccgctagg atagatatat tcataggggt cttgcgtagc
    54661 tgttggccca agacgctcca aaataccgtt gaataactgg cgttgaatgc gctggagtgc
    54721 ttgaccgctg ggtacgattg gttcgtccag cttttccatc tcattttctt cgctacgttg
    54781 aatttcagcg gaatcttctg tatcagtttc attttcttgt aattcttggt tatctattct
    54841 ttcatcaacc accacttgaa gaggtagtta cccttcgttc acatcgggga tcttttctgt
    54901 ttcaaaaatc aatgtgccgg tttctgcttc ttttgaataa acttttccga cataccattc
    54961 ttgaccatcg atcgtctcga cttttaggtc ttctccatct tcatcatagg aggatagacc
    55021 tgttccacga acgtttttta cggtaccaat cccggcggct gccgcatcga ttgctaaacg
    55081 tattctacct tcattggcac tttccttttt ttcaaaagcc agatgccatt caagggtttc
    55141 ttctttttca atatacttcc cttcaatttt tgcatagtcc tcatctaaga atacctcatc
    55201 ctcctttgtt tctgttgact cagcagctac atccagggtc gtcatatgat agactgactg
    55261 gaaatatggt ccaaatagca aactgacagt tactaactgc aaaagcttca attttaaccc
    55321 tttcacatcc ttatactcct ttcaaaaata tttgtcttct ttcacaacaa tcaacaaaac
    55381 aatgcccatc taatt
//
LOCUS       DAFFSV010000005        50917 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFFSV010000005 DAFFSV010000000
VERSION     DAFFSV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.005"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            38..883
                     /locus_tag="LR110_000237"
     CDS             38..883
                     /locus_tag="LR110_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4821244.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1164..2384
                     /locus_tag="LR110_000238"
     CDS             1164..2384
                     /locus_tag="LR110_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBL4821245.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2381..5101
                     /locus_tag="LR110_000239"
     CDS             2381..5101
                     /locus_tag="LR110_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4821246.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5085..6029
                     /locus_tag="LR110_000240"
     CDS             5085..6029
                     /locus_tag="LR110_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL4821247.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6098..7108)
                     /locus_tag="LR110_000241"
     CDS             complement(6098..7108)
                     /locus_tag="LR110_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL4821248.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7288..7632)
                     /locus_tag="LR110_000242"
     CDS             complement(7288..7632)
                     /locus_tag="LR110_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL4821249.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7625..8059)
                     /locus_tag="LR110_000243"
     CDS             complement(7625..8059)
                     /locus_tag="LR110_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBL4821250.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8197..8934
                     /locus_tag="LR110_000244"
     CDS             8197..8934
                     /locus_tag="LR110_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4821251.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8927..10138
                     /locus_tag="LR110_000245"
     CDS             8927..10138
                     /locus_tag="LR110_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4821252.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10342..11124
                     /locus_tag="LR110_000246"
     CDS             10342..11124
                     /locus_tag="LR110_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBL4821253.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11194..11856
                     /gene="trmB"
                     /locus_tag="LR110_000247"
     CDS             11194..11856
                     /gene="trmB"
                     /locus_tag="LR110_000247"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBL4821254.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11954..13708)
                     /gene="ade"
                     /locus_tag="LR110_000248"
     CDS             complement(11954..13708)
                     /gene="ade"
                     /locus_tag="LR110_000248"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBL4821255.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13840..14769
                     /gene="tadA"
                     /locus_tag="LR110_000249"
     CDS             13840..14769
                     /gene="tadA"
                     /locus_tag="LR110_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBL4821256.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14726..15775
                     /locus_tag="LR110_000250"
     CDS             14726..15775
                     /locus_tag="LR110_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBL4821257.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15772..16050
                     /locus_tag="LR110_000251"
     CDS             15772..16050
                     /locus_tag="LR110_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBL4821258.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16025..16495
                     /locus_tag="LR110_000252"
     CDS             16025..16495
                     /locus_tag="LR110_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBL4821259.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16512..16814
                     /locus_tag="LR110_000253"
     CDS             16512..16814
                     /locus_tag="LR110_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821260.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16831..17202
                     /locus_tag="LR110_000254"
     CDS             16831..17202
                     /locus_tag="LR110_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBL4821261.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17174..17533
                     /locus_tag="LR110_000255"
     CDS             17174..17533
                     /locus_tag="LR110_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821262.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17705..18712
                     /locus_tag="LR110_000256"
     CDS             17705..18712
                     /locus_tag="LR110_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL4821263.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18737..19921
                     /locus_tag="LR110_000257"
     CDS             18737..19921
                     /locus_tag="LR110_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBL4821264.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20358..21941)
                     /locus_tag="LR110_000258"
     CDS             complement(20358..21941)
                     /locus_tag="LR110_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL4821265.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22519..22662)
                     /locus_tag="LR110_000259"
     CDS             complement(22519..22662)
                     /locus_tag="LR110_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBL4821266.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22884..23363)
                     /locus_tag="LR110_000260"
     CDS             complement(22884..23363)
                     /locus_tag="LR110_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL4821267.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23436..23720)
                     /locus_tag="LR110_000261"
     CDS             complement(23436..23720)
                     /locus_tag="LR110_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821268.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23798..23992)
                     /gene="ssrS"
                     /locus_tag="LR110_000262"
     ncRNA           complement(23798..23992)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LR110_000262"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24064..25350)
                     /locus_tag="LR110_000263"
     CDS             complement(24064..25350)
                     /locus_tag="LR110_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBL4821269.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25488..26159
                     /locus_tag="LR110_000264"
     CDS             25488..26159
                     /locus_tag="LR110_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBL4821270.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26125..26613
                     /locus_tag="LR110_000265"
     CDS             26125..26613
                     /locus_tag="LR110_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBL4821271.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26626..27573
                     /gene="prmA"
                     /locus_tag="LR110_000266"
     CDS             26626..27573
                     /gene="prmA"
                     /locus_tag="LR110_000266"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBL4821272.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27574..28338
                     /locus_tag="LR110_000267"
     CDS             27574..28338
                     /locus_tag="LR110_000267"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBL4821273.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28571..30784
                     /locus_tag="LR110_000268"
     CDS             28571..30784
                     /locus_tag="LR110_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBL4821274.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30795..31241
                     /locus_tag="LR110_000269"
     CDS             30795..31241
                     /locus_tag="LR110_000269"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBL4821275.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31412..32671
                     /locus_tag="LR110_000270"
     CDS             31412..32671
                     /locus_tag="LR110_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBL4821276.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32760..33707)
                     /locus_tag="LR110_000271"
     CDS             complement(32760..33707)
                     /locus_tag="LR110_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL4821277.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34101..35393
                     /locus_tag="LR110_000272"
     CDS             34101..35393
                     /locus_tag="LR110_000272"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBL4821278.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35408..37177
                     /gene="aspS"
                     /locus_tag="LR110_000273"
     CDS             35408..37177
                     /gene="aspS"
                     /locus_tag="LR110_000273"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBL4821279.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37286..38161
                     /locus_tag="LR110_000274"
     CDS             37286..38161
                     /locus_tag="LR110_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL4821280.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38427..40310
                     /locus_tag="LR110_000275"
     CDS             38427..40310
                     /locus_tag="LR110_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL4821281.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40385..40669)
                     /locus_tag="LR110_000276"
     CDS             complement(40385..40669)
                     /locus_tag="LR110_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL4821282.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40659..41393)
                     /locus_tag="LR110_000277"
     CDS             complement(40659..41393)
                     /locus_tag="LR110_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBL4821283.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41522..42145
                     /locus_tag="LR110_000278"
     CDS             41522..42145
                     /locus_tag="LR110_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBL4821284.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42337..43458
                     /locus_tag="LR110_000279"
     CDS             42337..43458
                     /locus_tag="LR110_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBL4821285.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43465..46593
                     /locus_tag="LR110_000280"
     CDS             43465..46593
                     /locus_tag="LR110_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBL4821286.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46612..49806
                     /locus_tag="LR110_000281"
     CDS             46612..49806
                     /locus_tag="LR110_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBL4821287.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49952..50659)
                     /locus_tag="LR110_000282"
     CDS             complement(49952..50659)
                     /locus_tag="LR110_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL4821288.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16657 a         8919 c        10597 g        14744 t
ORIGIN      
        1 caataagcag tgaatttata atcaacgtat aggagacatg gatgaaatga aactaattgg
       61 tatagattta gacggtactc ttttgaattc tgaccagagc attagtcaaa aaaatgcaag
      121 aacaatgaaa catttaccgg aagactgttt tccattcatt tgttctggaa gagaagtaga
      181 agacattaat aatattctca aaaaatctgg tttaaggata ccagcagtag ggttaaatgg
      241 ttctgttgga tttgatggag aaaaaaagat atttgagtct tccttcgata aaaatgatgt
      301 aaaaaaaatt gattctgtcc tatcgaaatt tccaaccaaa atatatacaa atctcggaag
      361 ttatgaatct cagaattata aaaataaaat gaaaaaaatt tttcacgagg tagggagtga
      421 attttctatc aaagagctaa actatgagct tgagtatgaa aaatctattt gttcaatccc
      481 ttataataat attgaggaaa ttatagaaaa ggatgctatc agcatttaca aattttttat
      541 ttttattcct aatcgtcaat tgaaagctaa tatatttaac catttgaaaa agatgctaaa
      601 tgtgtctgtt actgaatcgg cacctgtaaa tattgaaatc gtacctcaaa atgtatctaa
      661 aggtgtggtg tttgatcgtt tggaatcaat ctacaattta aagaaacctt tgagaatcgc
      721 gatcggagat agtttaaatg acttatctat gtttgaaagt gcagatatta gttttgcaat
      781 gggaaatagt catcaagaaa tcatgaaaaa agctgattat gtcactgttt caaatgataa
      841 agacggagta tctcaagcat ttgaaagaat tcttaccata taaactaatt aaggtagtat
      901 agaacataga attctaaata cggaaaaatg gagcataaat tccagtacaa atagtacttc
      961 ttcagtgtca aacgaagcga atataacata gatatttagg attgtgacaa aaatcatgtc
     1021 acaatctttt tttcgaataa acttattttt tgaagtcggc catttatcgt aatttctttg
     1081 cataatacga acaaattttc ggttattgat gaaaaaaaac attgaaaatg gtatgataaa
     1141 agaaaaaaag gaggaaattt ttaatggtga aatttatcca tgcggcagat ttacatatgg
     1201 atcggtcatt tgaaggactg acaactcttg ataaaactgt acaggaaaaa ctattgaaga
     1261 cgaatctgac cgttttatca aatatcattg aacaggcaat aataaataac gttgattttg
     1321 ttttacttgc aggagataat tttcatcaaa atcgtccttc attaaaaata cagaaacatt
     1381 tttcagaaca gatgaaacgg ttagaaaaga accatatacc tgtatttata atattcggaa
     1441 atcatgattt ctatcaaaaa gagcggtatt ggttctcctt tcctaaaaat gtccatttat
     1501 ttacatccga aacggtagag acaaaaaaaa taacgataaa aagtggagaa actgtctctc
     1561 tcagtggatt tagttatcgt caaccgtgga ttcaaaaaga caaagtgatg gaatttcctt
     1621 ctagagaact cacagattac catattgggt tgtaccacgg ggaacctggc gtctctcaaa
     1681 aaggaaacta tgctcctttc caaccctcaa agatgcaaga aaagggatac gattattggg
     1741 ctttagggca tatccatgtg ccaacggtct taaatgaaaa gcaaacaatt gtttatccag
     1801 gtgcgcctca aggtcatact aaaaaggaac aagcttctac atctattcta ctagttgaat
     1861 taagtaaagg aagttgccaa atacatccga ttaaagtagc agaggtctat tggaagacaa
     1921 aggaaatatc attgaggaaa gcaagaacga caaaagaggt cattgttcac attcgtcagc
     1981 agctttcaaa agtagaggat ggatttagtt taattgagat aaaattaaaa gattatgacc
     2041 atctcaatac agatgttttg gaacggatac aatcaggaga gctattagac tatttgttag
     2101 aggaattctc agataggata aatgatttct ttatttggcg tatttctctc atagagaata
     2161 ctttttatac gaagacccca cttgctgctt ctgctaaatt gatggaacag ttatttcaat
     2221 actatcagac acctcaagat ttccaacaaa ttttaaatga agtatatagt catcaagaag
     2281 cggctagaat attgagcgaa ctgccggaat atcaagaaga aacacttgaa aaagccaagc
     2341 agctgcttaa tcaagatttt ttatttgagg aggatcagga atgaagattt taaggatcga
     2401 gattgctgct tttggaaaat ggcgtcaaaa aagttttgac ttttattcag gcaatcagct
     2461 gatctatggg ggaaacgaag caggaaaatc aacgatttat caatttattc aagcaatatt
     2521 atttggtttc ccttcaaaag gccgtaaaaa aaaggattat acaccaaaag acggctcagc
     2581 ctatggcggc aaaatatggc ttaaacatcc tgtatatggt gaatttgctg tcgagcgcta
     2641 caagcagcaa aatcgcggaa aatcgaaggt gtggctgggg gatcaagtgg gaagtgatga
     2701 gttactagaa aaactgatcg cacctttgac gaaagagtta ttccagcagg tatttacttt
     2761 ccagcaagag cagttgatgg aactagatca tttgagagaa aaagagctac atgatgcgct
     2821 gatttcttta ggcatcacgg gcagttctca agtattccaa aagcggcaag aatattatca
     2881 gaaagcacaa caattattca agaaaaaagg ccaaaaatta ctaatcaatc aaaaattaaa
     2941 tgaatggaaa gagctgcaag aaaaaattca tcaaaaacaa gaacaggaag cttattttgg
     3001 caagttgatc caacaagaac aggaatacga gtcgcaacag cagcagttat tggcacaatt
     3061 aaaagaggcg aacaaacaat tttcattagc cagacagcaa gaacttaatt tttctttata
     3121 tgaagaatgg caatctctca aaaaaaacag cactacaggt cttcctgatg agcaacttct
     3181 tttagatctg gagtctttct ccaaacggta tcagcaaaca aatgagcgtt tggaagaatt
     3241 agaagcaaca ctggaaaagc atagcgggat ggatcagcag tcagccagat attattttta
     3301 tttagagcag gagcaggaac taaaagatct ccagcaactt caatttacag cggaacaatt
     3361 aaacgaagaa tggcaaagat tacaaattga gactagagaa acaaattcag aattgaagat
     3421 tttggaacaa aggtggcatt ggcagaaaaa tccgttaccg gaagaatttt tatatgaaga
     3481 agaatggaag catcttctgg ctgaagaaag aaggattgct gatctagaaa gccagatttc
     3541 tgtgcgtctc caaatagcta aagaacagca agagatgatt gaagcagaag tgaatgactt
     3601 ggaagagacc tatccagcat tattacaaaa tcagtcagag atagcaccag tctcttcatc
     3661 taaaccgtat atgctgatgg ctggcggagc aataatcatt gcaggtatat ttgtaccaat
     3721 gcccttgaaa gtgatccttt tactaattgg tgcaggcatg attggctatt tcttctacaa
     3781 aaggcaacca aaaattaaaa ctaaaaatga tttctctgag ataaaaggaa tgtggcaaga
     3841 aaaactagga caacttgatc tagccttagc tcaattagct gaagcagaag aaaaacaaaa
     3901 gagaactgga gaagaaaaac agattcttca gcaagaagta tatcgttttg cggatcatcg
     3961 gcacttagga aagatggata cgctcaaact catgaaagaa tatggacagg aagtcataga
     4021 ttatcagaaa ttatatggcg tatttgagcg gaaacataaa agagaacagg aaatacaaga
     4081 aaaactacga caaatcgacc aaaaatttga gtttgttaaa gaatggttac cgatacaaga
     4141 taagacaatt tcagaaaaaa tggtattgct ttcccgtttt atacaagaga tggaacagat
     4201 gaaatttgcc agaagctatc agcaaaatac tttattgaag caagaaatca atcaattgaa
     4261 aaagaaacaa aaagtatgga tacaagaata cagcgaacaa ttgactcaag caggcttatc
     4321 ttatccttcg gaagtacctg tctttttaca aaaagccaaa caagccgaac agcagatgaa
     4381 aagatacaat gaattatcgg aaatgcttac tccactattt cctgaagagc tgacaaaaga
     4441 gaaattatct gtaagactgc gccaactgaa agaaaagcaa tcccaactcc agcaagaaga
     4501 aaaagaagca agtgaaaatc ggcaacgact ccaacttcaa gtagaagagt tgcaaagaga
     4561 cggcacattg gacgaacttt atcaacaagc tagccggcaa aaagcaaaat tacaggaatt
     4621 actggaggaa tggggagcac ttgaaatcgc cgggacattc ttaggtgatc tttcaacaga
     4681 gatgagtgaa agacaattac cccagttgtt aagagcagca ggtgattatt taggtatttt
     4741 aacaaaagga aattatcaga agatattact gaaaaatgat acactgactt taacagacgg
     4801 atatacagaa tttcccatct atgagttatc tacaggtacg aaagatcagc tgattatggc
     4861 gatgagattt gcttacctct cattagaagc taaccgttcg atatgcccga tcatcattga
     4921 tgatggctgg cttcattatg atcatcagcg aaagtatcaa ttagctgaac ttttagctaa
     4981 atttggagaa aagtatcaaa taatttgttt ttcttctgat aaagaaatgg taagctatta
     5041 ccatgagatg aaccaaatcg taaaacaatt agaaggagca aataatgaaa aaaattcgtg
     5101 atttagtaat cgatgagcag tttgaacaat ttgtcctgat caaaagtgca gatgtaagaa
     5161 tcgctaaaaa cggaaaaaaa tttattgctt ttactttcca agatacttca ggtactatcg
     5221 atggtaaata ctgggatgct tcggaggagg aaatcaataa atttacagcc ggcacagtcg
     5281 tttttctaaa cggtaaaaga gaggtttatc aaggaaaccc tcaagtaaag attcttcata
     5341 tgcgcttaac aagggtagat gagccaggag agcctagttt atacatggaa agagcccctt
     5401 tgaagaaaga agaaatggaa gaagaaatca atcaattgtt gtttgagatc accaatgcgc
     5461 attggaatcg tattgttcgc tatctactta ataaatatca aaaacaattt tttgagtttc
     5521 cggcagcaaa aagaaaccat catgcatttg ccagtggatt agcttatcat actttgacaa
     5581 tgcttcactt agggaaatca atcgccgatg aatatcctga gctgaatcga gcattgttgt
     5641 atgctggtat cattttgcat gatttaggaa aggtgattga actttccggt cctatgtcga
     5701 cagaatatac gcttgcagga aatttgatcg gtcatctggt attgattgac gaagaaataa
     5761 ctaaagcctg tcttgcgttg aaaatcagcg aagctgacga agatgttctt gtattacgtc
     5821 atatggtttt agcacaccat ggcttattag aatatggctc acctgtacgt ccaagaataa
     5881 tggaagcaga aatattacat caaatcgaca atatcgatgc ttcgatgcag atgatgttga
     5941 cttcgattcg tcaaacagaa ccaggaaaat acaccgatcg gattttcggt atggacaacc
     6001 gaagtttcta tgtaccaaaa gatttatagc aaaaatttat ttttttcatt caaaacaaaa
     6061 aaagcgagac ataagtcttc gctttttttg ttttctttta ttttgatgaa tcagttgttt
     6121 cttcagttga agattctttt gttgaatcag ttgattttgt gtcagaactc tttgttgaag
     6181 ctgtagtttc acttgagtct ttcgtacttg atgaagttgt tgtgaatgca ctcaacacat
     6241 tttcaaaggc atcatctttg atttttacat ttgcatcttt taattcttca ccgattactt
     6301 tagttgtgaa tgcattgtca cttagtttag tttcagtagc gatatctttt agttgatctt
     6361 tgtatttatc catgtcattg cctttatttt ggtttttcac cattttgaca acgtagtatt
     6421 ctgtcgcata agaagtagga tttgttgtag taataacatc ggagatctca ccgtctttca
     6481 atttgaaagc tgcttctttt acttctgcag gaatagttgt tgtcgttgag tcgaatttaa
     6541 ctttaccgcc atcttctttt gtctctgtat cagttgattt gtctttggct aatttagaga
     6601 aatcatctcc atcatccgct gattttttca catcttttgc ttcatcttca cttgataatt
     6661 tgatgatttg tgcttctact tctggatgga aagatttcca agcagttttc aaatcgtcat
     6721 ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc taaattgtta cgaatatatt
     6781 ctttataagt atctttcgta tatccagcag cttctaattg tgattcaaaa gtatcaccta
     6841 atgatttcgc ttgtttgtcg tattctgcat ccacttgctt atcggttact ttatcgccat
     6901 atttgttatt gaatacttta taaatgatca tccgttgtac taaggattgg ttcgatgatt
     6961 caagttttgc ttcatcatag aaatcagata cagtgatcgt tccgcctttc atcgttgcga
     7021 tatctttatt agtatcgcct gaacaagctg ctagagttaa aacagccaac gcactagtag
     7081 ctgccaaaat aattgatttt tttttcatta aaatttgcac tcctgtaatt agttattatt
     7141 tttatatatt aactatacca tatcccattt cctttaaaaa cgttgtcgtg gtagtttcaa
     7201 acaaaattca aaatttcatc acattcctag gaaatacgca aacttttctc acttatatag
     7261 ttaaatctgt tgagaaaata atcattatta tatttgttca ggatactctg gcagatcagc
     7321 ttttagtttt tctacttgtt cattgatttg tgtgatacgt ggttcggctt ggaatttaaa
     7381 atcttcgata tcttttttga ttgcttcttg gaaagcagga atcacagatt cagctgtttc
     7441 ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg ttatttaaat catttgtcag
     7501 agttgttgct tcttccaatt cttcgatcag tttctttctt gtttcattcc cgctcctggg
     7561 cgccaataac agacctccga cagtgccagc agcagctcca aataacagtc ctttgaaaaa
     7621 gtttttaatc atcgcaggac acctgcttta cgattgtttc ttgaatagct tttagttctt
     7681 ccgatccata actatcttga tgattgacaa agtgaataga aaaatcatcc tctttgctgt
     7741 agcgagggac taaatggata tgtgaatgaa aaacagattg ataggctagt tcacggttgt
     7801 tattcaagat attaagacct ttcatttctg gaaaagcttt ttccaatgct tgagcaatct
     7861 taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc atattcaaaa atatctgcta
     7921 catgacgttt aggcacaact aatgtgtgtc ctttcgttgc ttgagagatg tctaaaaaag
     7981 catagacctt gtcgtcttca tatattttat agcttgggat ttcttgattg atgattttgc
     8041 aaaaaataca gttttccata taaaacatcc tttctataaa gaataaatac ttcagcttaa
     8101 ctttaccata agttaaagct attgtaagga aaaaaacaaa ataccgtcat tttcttcgtg
     8161 gtataataaa taaaaataaa tggcggagga aaaagaatga gtttaattat cgatcatgtc
     8221 acaggggggt acggacatct ccctgtgtta aaagatatca gtttcgatgt acagcctgga
     8281 gagatagtag ggttgattgg actgaacgga gcagggaaaa gtacgaccat caagcacatt
     8341 atcggtctgc tggaagcttc aaaaggaacg atagaaatca atcatctatc cttaaagaaa
     8401 aatacggaag aatatcgcaa acaaattggg tatattccag agactccttc tttatatgaa
     8461 gaattgacat tgagagaaca tatcgaagtc actgcgatgg cctacgatat acctatggat
     8521 atagcaatga aaagagcaga aaagttgttg gagacttttc ggttatctaa tcgtcttgac
     8581 tggttcccag ctaatttttc aaagggaatg aaacagaaag tgatgatctt atgcgccttt
     8641 ttgatcgagc cttctttata tatcatcgac gaaccatttt taggcttgga tccactagct
     8701 atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag aaggagccgg tattttgatg
     8761 tctacgcata ttttagctac tgcagaaaaa tattgtgatc gttttgtggt cttacataac
     8821 ggagagatac gagcagacgg ttctcttgag atgttgagaa aagaatttca gctgccggat
     8881 tcttctttgg acgaaatcta tattgcttta acaaaggagc aggcacatga gtagtttttt
     8941 tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa tatatgcgtt atgtattgaa
     9001 cgatcatttt gttttagttt gtcttttctt gattggcggt gtgggattat attattctaa
     9061 ttggttgaaa accctgcagc caccgtttgc aattggaggg ctcataatct ctgtattttg
     9121 gatgctctgt ttattcgtgg gtaagatggc tacttttgca gaggcagcag atattgtttt
     9181 tttacttcct aaagaaaaag aaatgcaaac ctatttggaa cgagcttttc gctattcttg
     9241 catttttccg ttgatctttt tgatattggc atgcggattg gccatgccat tagtggtggt
     9301 tagtacggag caatcctttt cgatgttttt cgtctatctg atcatattat ggagtttgaa
     9361 attcagtcat ctgcaaatca agagagcaga attgtttcga ctagatcaac aagttattcg
     9421 ttattggaaa ttaggctggt ttttttcttc tctgatcttt ttgtttctat cattttattg
     9481 gctaatatgg gcaggaatag caggtgcatt agtacaggca agtctatatt actggttttt
     9541 gtggaagcaa caagaaaaag gattggattg ggagaaaatg gtgcatgtag aagaacatag
     9601 gttgcatcag atctatcaat ttatcaattt attcacagat gttcctgaaa ttacggcaaa
     9661 gatcaaaaga agacgctatt tggatcctat attgactcat attaaaagag aatcaaaaaa
     9721 cacttatctt tacttgtttg caagaagatt ggcaagaggt tctgatttta gtggtcttta
     9781 tttccgatta gtaattatag gtgggttatt gattgccgga gtagtcgact tttattttag
     9841 tctgattatc ggtttcttat ttttatattt gatcggtttc caactgttac ctttagcgaa
     9901 ccagttccgc tatatggttc tcgttcagct ttatccaatc cataagaatc agaagaagca
     9961 ggcgattcag tatctactca catgggtatt aattatcgcg tcggttattt ttggtttgat
    10021 tgctgcagtc gtattagatg ggcagcagcg atgggttccg ctagcggctt atttgattgt
    10081 tactatgctc tttgttaaac tatatgtacc aactcgtttg aagaagatgt cagactgaaa
    10141 acaaagctgt gtagggtaaa aatgagaatg aaaaatagag cgttgacagt aagaatgctg
    10201 ttcgttctat tttttttgag aaaagctggt tttttgaatg gcaattaaat gaaaaagaac
    10261 gtatatgtgc ttgacgtgta gtgttcaggc gactagaata ataagtactg caaaacaaag
    10321 agtaagagga cgggagaatg catgatggag tttcagttag accaagagtg gcggttacgt
    10381 cccatcaaag gcgacacagg aaaaacatac ataggcatgc gtgatgagga caaagtcttt
    10441 attaaacgaa acacgactcc gatgttggct gctctttcaa gggaaggaat tacaccaaaa
    10501 cttgtttgga caaaacgaac ggggaatggc gatactttga cagcacaaga gtggctggaa
    10561 ggtcgcttgc tgactcctaa tgagattggt caaagaaacg acgtgattga tgttttgtac
    10621 catttacatc attcaaattc tcttaaaacc atgctgaccc gtatcggtgg tcgtgtcatg
    10681 acgccggaga agatgctgca aaaatatgaa aaagaattac cggaagaact ccagaaaaat
    10741 caatttctgc aaattgtcca tcgttttctt cgtgagcaga tcccgtcaat agataaggaa
    10801 aacttatcag tagtacacgg agatgtaaac catcgtaatt ggttagtatg taaaaattat
    10861 ttatatctag tcgattggga ttcaatcatg tttgcagacc ctgcggtgga tattgcaacg
    10921 attttaggaa attatgtgcc actgtctagc tggaatcagt ggctgatcag ttatggcata
    10981 cgcccgactg acgaaatgct tgaaaaattt cattggtatg cggccatgaa tttgctccaa
    11041 gaaatcacca gatattgcaa acgtgaagat catcgacgga tgaacgaaga aattttacaa
    11101 ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc tgcggagatt tggccagacc
    11161 tttttaatta gaggaaagga atggagataa ataatgcgtg tccgaaatcg tccgggagca
    11221 gcggaaatgt tagcggctca cccgaatttt gtaattagtg atccaacatt gtggaaggga
    11281 aaatggaatg aattgtttga aaacgatcat ccaatccaca ttgagattgg tatggggaaa
    11341 ggccagttca tcactggaat ggcaaaagcc catcctgaaa tcaattatat tggagtagaa
    11401 atgcaggtca gtgttgtttc gattgcacta gacaagttga tcgaacaacc gcttcctaac
    11461 ctgaaacttc tacatgtgga tggatcagcg ttgacagagt atttcgctga cagcgaagta
    11521 gatcaaatct atctgaattt ttccgatcca tggccaaaaa aacgtcatga aaaacggcga
    11581 ttgacatata agacttttct tgctgtagat gaacagattc ttcgtccaaa tggagaaatc
    11641 catttcaaaa cagataatca aggattgttt gagtattctc tggcaagttt ttctcaatat
    11701 ggcatgatcc tcaaacaggt atggttagat ttacatcaaa gtcaatttga aggcaatatt
    11761 atgacggagt atgaagaaaa attttcttca aaaggtcaac gtatttatcg agtagaagct
    11821 caatttcaag ataagaccaa aaagaaaagt ctgtgaaact aactttctgc tttcataaaa
    11881 acattaatat ccataaataa atctcagaaa atgaacatta cagatgtaat gtattttctg
    11941 ggatttattt gatttaaaat gtatttattt catcggtttt tccacaaggt ggaatcaatt
    12001 ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt caattcgggt atgacagata
    12061 atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt agctgctcct agttgatgtg
    12121 ctttagttat taattcattc aactgttcat agacgatcga aaaaggttcc tgtgtcatga
    12181 ttccgcctat aggaagagaa acacaagcta tttcctgctg tttgttgaca acggtcatcc
    12241 cgccaccttt ttgaatcaaa agattagcag catacagcat atcctcgtcg tttgtcccaa
    12301 cgacaactag attatgcgag tcatgggcaa tcgttgtcgc tatcgctcct tgtttcagct
    12361 gaaatccttt cactatgcct aatccaatgt ttcccgtttg atggtgacgc tcgatcaccg
    12421 caatctttaa ttggtctttt tcgattgaag gaatgaatcg ttcgccttgt cggtccactt
    12481 tttctaccaa atctttagtc actagactat tcgggataat ttcgatcaca tgggcttgtg
    12541 aagaagaaag aagcagctct aacgaatgtg gatttaatgg ttgagcgttt agttttggca
    12601 gacaacacgt atatgaattt gaatgatcag caaataaatg gtcttggagt tgcccagatt
    12661 gaacaactaa tgttccctta tagaaaactt catgaattgg cagcttggta agatcgtctg
    12721 ttaagaagaa atcagcttga tacccagggg caatcgcacc tagtgttttc aaatcaaaac
    12781 attctgctgc attcaatgtt gccatctgaa tggcttgtaa tggatcgatt ccaagtgcaa
    12841 tagcttgacg gacgatatga tcgattgatc cttcttttac taaatcattg atcagtttat
    12901 catcggtgac aaagaagcat cttcttgaat tttcaggtgt gatcgccggt agtagcgatg
    12961 ccagatcttt tgcaacggtc ccttctcgaa ccattaaata cataccaaga tccaaacgtt
    13021 ctttagcttc ttttgcagtt gtagcttcat gatccgtccg aatacctgct gcaggataaa
    13081 catttaactc ttccatgccg ataccagctg catgcccgtc aatttttttc ttatgctggt
    13141 gcatcaattg gatcttttct aggatgtctg tttcattatt tgctactgct tgatagttca
    13201 tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa aggggctaat ttttttgcat
    13261 ttaaatttgc accattggtc tcaaaagggg ttacaggtac acaagaaggt agcataaaaa
    13321 agatattcat gagtgtttgt cttgcatctt ctatcataaa ctcaagacct gtactcccag
    13381 caatgtttcc tatttcatgg ggatctgtca ctacagtagt aactccatga agcaaggata
    13441 cttttgaaaa ttcgcttggt gataaaagtg agctttcgat atgcacatgg ccatcgatga
    13501 atcctggcac tagatattgc ccattagcat tatgtacgct ttttccttcg tattcaccga
    13561 ttcctacgat cattccttcg cagattgcta catctttttc catgatttct ttcgtaaaga
    13621 cgttgacgat cttagcatgc ttgatcacta ggtcagcagg ctttttttta cttgctactt
    13681 cgatatattt ccttaatgtt gtttccaatg gagtgcctcc caagataaat ttttataagt
    13741 ataacataaa ttcggctttt gccaatattt ttttgtaaat tcattttatt tgaaacaaaa
    13801 agatcaaaaa gacgtatgtt ttttgtggag gggagaataa tggaaataaa acgattatcg
    13861 gatcaaatga taagtgaaag ttttgccaaa catgcccaag acgtatatat attgccagct
    13921 tcagaagtat tttacatcta ttttcgctat ggaaaaaaac gaacattctg gaagaaagtc
    13981 tcttcggcag atggacagca actgatctca agatttaagt atttgggaca gatggatatc
    14041 ggagaaaaaa gaaaagctca gttaggtgca tttacttatt ctttagaaaa acaggaagtt
    14101 cgtctgcggt tgtcttctgt aggagattac cttcaacgag agagtttagt gattcggcta
    14161 ttatacggta cttttgaaac accccatcgt tgctttagtt ctcgagattt tgcaactatt
    14221 ttacagaaaa ccgtcagacg tggattatac cttttcagcg gaccagtcgg atcaggcaag
    14281 acttctctaa tgtatcggtt ggctttggaa gaaaaattgc aagtgattac tatcgaagat
    14341 cctgtagaga tagaagaacc gttttttctg cagctacaga tcaatccgaa aatagcacag
    14401 acttatgata cacttttgaa actcgcatta cggcatcgtc cagacttgct aatcattggc
    14461 gagataagag atgaatcaac tgcacatgct gcggtaagag cagcactaac agggcaccgg
    14521 gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat tggtcagaat ccatgaactg
    14581 acaaagaaaa aagaagagct atctgaatgt ttagctggtg tcatttatca agaacttttg
    14641 gaagatgcca atcaccatcc ttctgtttta tggagttatc aatttatgga ggaagaatca
    14701 gtaaaaaaat catgggagga aagttatgaa gaagctcagg aaaatatttg gcagtcagaa
    14761 ctattctaaa ggattaacaa gaagccagca aaaccagttt gcacaaacca tgggagaact
    14821 gcttttatct ggttttagtc tgcaagaatc cctttattta ttactcacga tccgttcgtt
    14881 tccacctgtt attttggcaa atatccaaca tcagctaaag gaagggcaag cgtttagtga
    14941 ggtactttcg caaataggat atggagcaga gcaactggta cagatcgagt tagctgaacg
    15001 tcatgggaat ctgccgacaa ctttgttgga aattgccaaa cagtttcggc ttgttgaagg
    15061 atttcgcaat gatttgaaaa agctgcttgc atatccgatg ctattgctca cctttttgct
    15121 aggtatcttg tttagtatgc ggttgttcgt tttgcctcag cttattggtt caggaatggt
    15181 catgcaagag cattggggta ttcagctgat acaagtcttt cattggtatg ttctactatt
    15241 catcctagcg gttactctat tggctttact agtaaggtat cgtctgatga agttgtctcc
    15301 tgttgatcag agtgaatgga taagcagaca attattgatt ggaccgatat attccttgta
    15361 tcaatcggct tatttgtcac tagagattgg aaagcttttt catgaaggtt tcgaattaag
    15421 gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt ctgattcagc atcttgcctc
    15481 taagttgatt caaggattgg aggcaggtat gtcattggct gagcagttcc agaattatcg
    15541 ttttctatca gaagaattca gtaaaattgt tttacaggga gaggcaaagg gaagtttagg
    15601 taaagaactg attttttaca gcgaatggac aagaaaacag cttttcaaac gtcttcatca
    15661 ctggatgcat tttattcagc caatcatttt tttgggcgta gcggctttga ttttattagt
    15721 ttatgccgct gttttattac ctgtttacgg aagcatagag gaggttttgc catgaaaaag
    15781 agaaaaatat cttattatag cggttttaca ttgatcgaaa tgctaatcgt cttactgatc
    15841 atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc gttataggaa tcatgtagac
    15901 caagaaagtc gtgaagcgat catccagttg gtagacacac aaaaagagct ctatgcgctt
    15961 caaaataatg gaagagttcc tactgtggag gaattgttga atgaaggcta tatcaaacga
    16021 gaacatgcag aaatctatca gcggccttga aggtaaggtt ttagcatcag gatatacgtt
    16081 gtttgaatca ctcttactga ttttattact tacaacgatc ctttcttttc cagttctcgc
    16141 attttctgca tggaagaatg aattggcagt ctatcaattt ttcggccaat ttgaaaaacg
    16201 aatctattct acgcaaaaaa tcgctattgt gaatcagatc caaacaggct tttattggaa
    16261 tagtgaagag aatcaaatca tttttcatgt tccgaatcca gataaaataa attgggaagt
    16321 attagatatt cctgatgaga ttaccttgaa acgccatgct tctattactt ttgctgctag
    16381 aaccgggaat gaaagttcac taaaagctta tcaattttat tgggaagaaa aagagcggac
    16441 gattacgtat caattccaga tagggagtgg tcgctataca aaaagaattg aataaaacag
    16501 gatttgtttt gttggagtcc cttacagcgc ttatgatcgc ttctgtctgt gtctacttag
    16561 tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa aagtggggag gaactgctat
    16621 ttattcgaat gatttatgaa gatatcaaga cgcagcggat gtatcacaaa agaccagagc
    16681 gaagggaaga aatgatccca tacgatatcc aaattgtcga taataatgga gtaatcgaaa
    16741 aagtgtttgt caatatggag aaagaagcga tcagtattga aaaaacaagt aattccagca
    16801 tttacgctga ttgaatgtct aattggactg atgatattga gcagtctatt gctcacattc
    16861 aatcttttga ttcagcaaac catccaagta agtgaatttt tgcagcgtaa agatcaaaaa
    16921 gagtggctaa tatttttagc acaattagaa gaagaactca agagcagtca caatgtctct
    16981 gtgaaaaatc agcaactgca ctacacgatc gaggataaac agtatatcat cgaaagatat
    17041 caggcaatga tacgtaaaag aagaacttcc ggcggacatc aacctatgtt aacaagtatc
    17101 actgagcttc aattattgga aaaagaacac acaatttctt tttatgtaca ctttgaaaat
    17161 ggagaagatg gatatgccat atggacaaaa aacgatcagt agaaaatgtt ataagggctc
    17221 tattttattt tctgtcttgc ttttatttct tttatgtacg tttttattga tggcagcatt
    17281 agaaagctac cgtttgtcaa tggatcttaa tatacgaatc aaaaattatt atatagcaaa
    17341 aataatgaag cagatggctt tatcggaaca gtctacgctt gccgaaagtt cggaaggagt
    17401 tttttactac acaactggat ctgtaacata tcagtgggta caacagtccc tattcttaga
    17461 ggtggaagtc tcaccgttta tctttcggtt cacagaagaa gtaaaaaatg atacagatac
    17521 tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt tctgttataa cactgttttt
    17581 aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg aaaacgattg aaattttgtc
    17641 aattgttcga tagaattaaa caggtgaaaa aattgttata atatatctag aaatgaggtg
    17701 caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa aacctggaag ctattcagct
    17761 tttacagaat gcattgggaa cttccttttt agaagcctat gtcgaaaatg cagaaaattt
    17821 aatagatgat tatcaagtac gtgtggtgga tggagtgccg acaaaagaga caacgcaacg
    17881 gatcacagct ctttatgaag agttgaagaa actttctttc gagccagaag aatggcgcag
    17941 actgtctcaa ttacttttat tgaagggcag ccagacagaa catttgcagg cgaaccatca
    18001 actcactcct gatagtatcg gttttttatt tgtttttctt attgaacaac tatacacaaa
    18061 taaaaaagca ccggttaaga ttctggatat tgcggcagga atgggcaact tgttattaac
    18121 agttctttta aacctatcga atgcggggta ccaaacagaa ggaattggtg tggatattga
    18181 tgatacattg ttggctgtcg cagcttctac aagtgatttg acacaagcaa atgttcaata
    18241 ctttcatcaa gacgggttgc aggaattatt aatagatcct gtcgattttg caatcagcga
    18301 tttgccaatc ggttattatc cgaatgacga aaaagcaaaa gagtttttaa caagtaccga
    18361 agaaggccat agttatgcac atcatttatt gctagagcaa tcaatgaaat atgtgaagcc
    18421 ggatggtttt ggtttgtttt tgatgccgag tggattttta gaaacggatc aaagtgaaga
    18481 aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga atgattcaat tgccagatga
    18541 attgttccgt aataaacaat cgcaaaaaag tattttgatc ttacagaaaa aaggaccaca
    18601 agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct tcattaaaag aaccagagaa
    18661 ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca tctgctctgt aatcattcaa
    18721 cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat gctgggagtt caagtttgaa
    18781 atggcaatta tatcaaatgc cagaagaaac cgtagttgcg aaagggatcg tcgaacgtat
    18841 cggtttacaa gattctattt ttaccatcaa gtacggcgat aatcaaaaat tcgaacaaat
    18901 catggatatc gaaaaccacg aagtagcagt aaaaatgctg ttagatcagt tgattgaact
    18961 gcaaatttta agctcttatg atgaaatcac cggagtaggg caccgtgttg ttcacggagg
    19021 agaaacttat ggggattcag tagtgatcga tgagaaagta atggaccgca ttgctgaatt
    19081 agcagaattt gctcctttgc ataatcctgc aaacttaatg gggatcaaag cgtttaaaaa
    19141 gattttacct gacattttga gtgtggctgt attcgataca tctttccact caacaatgcc
    19201 aaagcaaaat tatttatata gcttaccggt gtcttattat gaagatttca aagtaagaaa
    19261 atatggcttc catggcacaa gtcatcgtta tgtgtcggaa cgtgcggcaa aattattgaa
    19321 caaaccgatc gaagaactaa aaatcatcac ttgccactta ggaaatggtg tatcgatcac
    19381 agcagtcgat ggaggtaaat ccatggatac ttctatggga ttcacaccat tagctggggt
    19441 gacaatgggt acacgttcag gtgatatcga tccagctgtt ctcccatatt taatggaaaa
    19501 attagagatt gatattgatg aaatgatcaa tgttttgaac aagaaatctg gtctgctagg
    19561 cctaacagga ttgtctagtg atatgcgtga tcttgaaaag aactatgaaa aagaacatat
    19621 tcgtttagct tatgacattt tcgtggaccg catccgaaaa tacatcggcg gttatgtgac
    19681 aacgatgaat ggtgttgatg caattgtctt tactgctggt atcggcgaga atgacggaca
    19741 tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt tgtgaaatcg atccagcgtt
    19801 gaatgaattg cgtggtgaag aagtagatat ctctacaaaa gaatcaaaag taagagtatt
    19861 agtgatccca acagatgagg aactaatgat tgcccgtgat gtggagcgtc tgcgcggcta
    19921 agccaaagca ttcgttagga taaaagatga aacgcatgca agagggaatt cttgcatgtg
    19981 ttttttttgc ggagaagacc gcaaaatggt aatttaactt caatttccct tcttttttac
    20041 aggatcttct tagggaattt tgcttggaaa agtggtcaaa agggaaaaga ctggcatttt
    20101 atttattgat tttcaggaaa atcccaatat tttattttca ttcatcttta taagggacat
    20161 tacatatgta aaaagaaaaa acatttttag atcagcctcg tgggctgtcg actttattct
    20221 tcgctttatg tttagctaga aaaccgatcc gaaacattca ttgatcgaat aataaaaact
    20281 tgattttcca cctgtacttt gcctgaatcg tgaaagcaca ggtatgaaaa tcaagttttt
    20341 tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg gctggcggct gatctgctcc
    20401 tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa agtagcggtc atggctgact
    20461 gctaggagcg ttccttgata gtcttgcagc atttcttcaa tttcttctcg agcatagctg
    20521 tcgaggtgat tggttggttc atccaaaatc aggacgttga ttttttctaa aaagagtttc
    20581 agcaacgcca aacggatttt ttcaccaccg gataaatctt tgatccgttt acccacatcg
    20641 ctttgataaa agccactttt ggctaattgc cgtcgagcga cttcttcgtt tccagtcaat
    20701 gtgatggcgt aagccagcaa acggctatcg ggatcggcaa actgcagctg ctgcggcaaa
    20761 taaccgattt gcaagctact acctaatttg atggtgccac cgtaaggctt ttcctcaccg
    20821 agtaataatt tgagtaaggt tgattttcct gcaccattgt cgccgatgat ggctaaatga
    20881 tctccacgat agactgcaaa actgctgtct ttgaatagta cttgatcagc gtactgctgg
    20941 tgcaagtttt gaatgatgaa aacttctttg ccagattttc caccattaga caagctttgc
    21001 agccgatttt tcgggggagg tggcggtttg acgagtgtca gtttcgccag gcggcgttcc
    21061 agttctttgg cttttttgaa gaaggactca ttgtctcctt catgtgccca ctgacgaaaa
    21121 cgacgaatca tgactttcaa ccgctggact tctttttgct gcagttcgta attttttgtc
    21181 agctcagcca agcgagcttc tttcaagact cgaaaacggc tgtagtttcc gggatactca
    21241 atcaatgcac cttcttccag ttcccatgtt tttgtggtga cttgatcgag aaattgccga
    21301 tcatgagaaa cgaccagaac cgattttttt gtattctgta aatagttttc caaccagcga
    21361 atactgttaa gatctaaatg attggtcggt tcatcgagta aaagtaaatc ggcatcggct
    21421 gctaaaattt tggctaaagc gactttaact cgttcgcctc cgctaagttc tgttaaccgt
    21481 gcttcctgac ggtgtggaat ccctaagcct tgcaagatcg ctgcaatccg gtcttctaat
    21541 acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac cgtaacgggc cagacacttc
    21601 tctaagtttg tatctggttc tgtcatcaat gcttcgtagc gactcatttg ctgtttgagg
    21661 tcctgtatct cagcggcaga atggagcaag tagtggttga cggtcgcttc ggaaactgta
    21721 agttcttgtt cgaccgtccc gatctttaag ccttttttgc gactgataac accgctgtcg
    21781 acaccttctc tgccggtcaa gatttgtaaa aaggttgatt tgccagttcc gttgataccg
    21841 actaaggcaa ttttttcacc agcttcaatt tgacaggata aatcctcaaa taatggattc
    21901 ccgccgattg tttttttaat agaaattgct tgtatgatca tgatctctct ccttgagacc
    21961 actcaaaaag gccatggtta tccctggcct gtgttattta aactcagatt atcatgagaa
    22021 aaataccttg agatggccat tgattttacg tgaattcaac tgttttggac aaacttgtcc
    22081 cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc gatatggtat cgttggtaat
    22141 tttcattact tgttatttta gtcatgcgca tcatagtatt ttccctcctt cgtgtagttg
    22201 tcttttccag tgtacctgaa agtgtcagaa agttcaacta gcgctgacat ttacttaata
    22261 tttatcgatg tgtaattctg tcagagagct ttttttatct gactgagggt aatgtctcgt
    22321 ggaccacatc attgtattct gagctaaacc agcgttcagg cgaaatcata tggtgtttga
    22381 taaaaaactt tgtctacatg acttcctttt gccatttgct taataccgtt gcattaaaca
    22441 gctcttctct ttggaaatct caccaacatc gcgttcagga gtatcgttgt ggttgcaatg
    22501 gcaagcggta gcctttcatc aattcttgaa tgatttaccg aatgtagcac ttgccttctc
    22561 gcggtacccg aacccgaaat aaaattttag tttggcgata aaatcggtat ccgccatcga
    22621 tacttcatat tttttcgtat cagattcatc ggaaacttcc acattgacga accaataagc
    22681 tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac taatcgccct ggtgttcact
    22741 ttttttattt tacttgaact cattgactgt tttcccttta ttttaaacat tataaagacc
    22801 gatgagtgct tgccgcgtct tttgttttta tcacggtttt ttgtgcaaaa aaacctgccg
    22861 atagttggca ggtcgtctac gtgttaagct ttggtgtggt ctttagctgg caattggttt
    22921 tctaaatcag ttcggacaac aagcacatcg caagctgcgt ttctaataac atattcagaa
    22981 acagaaccaa taaataaacg ttctacagca ttcaatcctg tggctcctaa cataatcaaa
    23041 tctacttgat tatcttctgg gatttctctg gcaataattt gttttggtga gccatattct
    23101 acgacagagg ttacattatt caagccagct ttcttcgcat tgctttcata atctgccaat
    23161 gtttgctttg ccatttcagt agcttgttct gcaagcattc catcaaagga tgaaacggtt
    23221 tggaaagaac gtgtgtcaat gacatgtgct aatagaagtt ctccgttatt tcggatagct
    23281 acattgaccg cctttttaaa agctaattca gcttcatcag aaccatctac ggcaaccatg
    23341 atttttttgt attgttgtaa cataagcatt cactcctcaa gtcaatcgta ttgtactctt
    23401 ttattgtaga acactttagg gaaaaagaga attatttact gctttggttc agcaattttt
    23461 tttgcaaatc ggatactgaa ggtggcagaa acaataatta cgatcagtaa aaagaccaag
    23521 gcaataaagc gttgattgaa aaaagctgtt aaaataccca ttacgcctaa aacagcatat
    23581 gcagcaccgt aaaattgtag gaatccttga tttttatcat ttttgttgat caatacaaaa
    23641 aagatatcct gtttattaaa taaataatag ctaacgaata ataatactaa agtggcgata
    23701 atcattaaaa tcctaatcat gaacaaatcc tccaaaataa gcaacaaata gaaaaagcaa
    23761 tttgccaaga gcaaattgct ttattcttta aaagatggta atctccgtta atgccgttgt
    23821 ttgtcctgta gtattgatcc cgcgaattca agcaggtggg tcccatggtc gtagcttcga
    23881 actaccaaat gataaggcat gttaccagca gtgacacaag acgggtccca agatatcaat
    23941 aaaaatgttc ggtcaacaca caaagaggac aattcgaaca tcacagattt cccacctcta
    24001 tagtagcata tgaatttaaa aagagcaatg agagagctcg cagaaacaga accgttttct
    24061 tgtctagttt tttggaggat gctttttcca ctcttttata cggtaatact gttgacccag
    24121 agcctgttca tatttccctg tatcttttgg ctgataatac tgagcattct tcagtttatc
    24181 tggtaaatat tgctgattga cccaagcctg atcaaaatga tgaggatact gataacccac
    24241 gccacggttg agtgatttcg cacctttgta atggctatct ctcaaatgat caggaacatc
    24301 tccagctttg ccctctcgaa tatcagcaag tgctgcatct aatgccatgt aggcagaatt
    24361 ggattttggc gacaagcaaa gatcgacgac gacgtctgct agtgggatac gtgcctcagg
    24421 caatcccaat ttttcagcag ctaatacagc gttcactgta cgagccgcag cagcaggatt
    24481 acctaaacct atatcttcat aaccgataac catcagacgc cggcaaatac ttgccagatc
    24541 acctgcctca actaaacggg cgagataatg aagtgcagca tccacatcac ttccacgaat
    24601 cgatttttga aaagccgaaa taacgtcata atgagcatct ccatttttgt catgggtaag
    24661 agcttttctt tggacacatt cttcaatgat cgacagtgta agatgaatcc ttccttcttt
    24721 atctgaagga gtcgatagtg tagccagttc cagcccattc aatgcactcc gcaaatcgcc
    24781 gttcgttgca cgcgaaaggt gaagaagagc atcttcgtct aactggattg cttgttgtcc
    24841 aaggccgcgt tctttatccc ttaaagcatg ttcgactgcc aactggatgt cttgttctgt
    24901 taatggtttg acttcaaaga tctgtgtacg gctgcggatc gctggattga tggtaatata
    24961 aggattctca gtagtcgctc cgatcaagat gattcggcca ctttctaaat gaggaagcag
    25021 aaaatcctgt tttgttttat ctaatcggtg tacttcatcc aataagagga tcactgtacc
    25081 actcattttt gcttcttccg ctacaacttg tagatccttt tttgaatctg tagcggcgtt
    25141 tagcatgcga aatgcataat tagtagagcc ggcgatcgca cttgcgatac tcgtttttcc
    25201 agtaccagga ggtccatata gaatcatgga agagagcatg cgagcatcga ccatgcggcg
    25261 tatgattttt ccttccccga ctagatgctg ctgaccaacg acttcatcga tcgttcgagg
    25321 tctcatgcga taagccaatg gctgttgcat aggattttcc tccttcttgt ttttacgtcc
    25381 cttaattata tcatatttag caagatgcct agaaagaaca gccttctgtt gttttgtttt
    25441 catagcttat ccaactaata ttgtgtatga taaagtaggt gagaaaaatg acggataaac
    25501 aaaagataga tgaatttttt tggaataaaa cgacgccaga agtggcacgg gatttattgg
    25561 ggatgtattt ggagcatgac acaccagaag gaagattagc tggatatatt gtagacgcgg
    25621 aagcatacct aggtccagaa gacgaggccg cccatagcta tggtttaaga agaacaccgc
    25681 gtgtgcgggc aatgtatgaa aaaccaggta cgatctacct ttacacgatg catactcatc
    25741 ggattttgaa catcattact cagccagaag ggattcctca aggagtaatg atacgtgcaa
    25801 tcgaacccgc cgcaatgatt gatcaaatga gtaaaaatcg tggcggaaaa actggtccag
    25861 atatcagcaa cggtccggga aaattagtgg aagcattagc aattcctcaa gaactttatg
    25921 gtcagtctat agcagatagt tcgttacgct tagtttttga gaaaaaaaag acaccaaaaa
    25981 agatcaatgc tttgcctaga atcggtatcc caaacaaagg tgtatggaca gaaaagccgt
    26041 tgcgttttgt tgtgtcggga aatccttata tctctttaca aagaaaaaac caaatcgaga
    26101 aagattgggg ttggaggaaa gaaaatgaaa aagaaggatc tattaacata tttagatgaa
    26161 ataattgaaa aacaagtgac agactatgat gtagctttag attgggatac aaaaaatcat
    26221 acgatcgaga tcgttgttcg cttgtttgca gaaaataaag cacaatttga gattgatgat
    26281 gcagaaggga tcgtttcgca agaaccaatt atcgaatttg aagacggcgt acttcttttt
    26341 aatcctcaga aatctgtttt tgatgaacgc aattatctag cagtaatccc ttatgaagga
    26401 aaaaaagggt tggcaaaatc agtagctgat tctttagtgg aatatctaaa tgaagtatta
    26461 gctcaagggc aaagcgattt gcttgatttt ttggatgaag acgatgacga agctgtattc
    26521 gagcttcact gggataatca acaactagca gaattagtag aagtaaaaca gcaaaatgaa
    26581 gcagatactt atttaccgta cccaagttat taaggagtga cagatatgaa atggaatgaa
    26641 gtaaaagtag aaactgaaag tgaagcagtc gaagcagttg ctaatatttt gatggaggca
    26701 ggggcgagtg gtgtcgcgat tgaagattct ttagatgttg aacattttca gtcggatcaa
    26761 tatggtgaga ttttagataa aaatgatttt acatcgctga aagaaggcgc actagtcatg
    26821 gcttattttc cggaaacgac tttcttaccg gaaatcttgc cttttatcaa agatcgtatc
    26881 acacaattac ctgagtttgg tctagctatt ggaaaaaata aagtaacagt tagtgaagta
    26941 gaggaaagca actgggcaac agcctggaag aaatactatc atccggtgcg tattacgcga
    27001 atgctgacgg ttgcgccaag ctgggaaaac taccagacag aagatcctct agaaaaaatt
    27061 atcacactag atcctggtat ggcatttggc acaggcactc atcccacgac ttccttgaca
    27121 ttgacagcac tagaaacagt cttgcgtggt ggtgaaacat tgctagatgt gggaactggg
    27181 tcgggcgtgc taagtattgc agcaaaatat cttggtgcaa aagatgtcta tgcctttgat
    27241 ttagacgaag tagccgttcg ttcggcgaaa gaaaatatgg atatgaatga aatagccaaa
    27301 gatgtccatg tatcagctaa cgatcttttg acaggaatcg agattgaaag tgatgtaatc
    27361 gttgcgaaca ttttagcaga tatcatctta ttgatgatcc cagatgcttg gcgtttactg
    27421 aagcaaacag gcacgctgat cgtttcagga atcattgaag agaagaagca aatggttctg
    27481 gatgccatgt tagaacaagg gtttatcgtg gatcaaattt tccagcaaaa agactggtat
    27541 gcaattattt tgaaaaaacc agaggtggaa tagatgcagc ggtatttttt aaaagaatca
    27601 tatgaatcaa aagactattt tgttttagaa ggggaaaact atcaccacat tgtacgggtt
    27661 atgaggatgg aacccgagga tgaagttttt ttggcattca atgatcaaat agccattgtt
    27721 gcagaaatca cggagattac tgaagaggct gtttatctga aagaaattaa aaaagaagaa
    27781 aatcaaaagg aactgccaag ttcgattacc atagcgagtg gctatcctaa aggggaaaag
    27841 ctggattgga tcgttcagaa atccactgaa ttaggagcgc aagcttttct tggtttcccg
    27901 gcatcagcct ctgtcgttaa atgggatgca aaaaaacgta tgaaacgaca acaacggctt
    27961 gaaaagattg ctcaagaggc agcggaacaa tcccatcggc agatcgtgcc atcagtagag
    28021 ttattggaaa cgaaacaaga tttatttgag cggctagatg attatgacgt ggtcttggtt
    28081 gcttatgagg aatcagcaaa gcaaggcgaa aaatcaaatg cagccaaaat tcttagtgaa
    28141 acaaaaccag atagtcgaat cttagctgtc tttggaccag aaggtggatt atcccgcgat
    28201 gaagtcgatt attttgtaga acacaaaggg atactttgtg gattaggacc aagaatcctt
    28261 cgtacagaaa cagcacctct ttatttatta agtgctgcta gctatcaatg ggaattaatg
    28321 aatccttctt tcagctaacg ttcttcatta ataaaagagt atcgacagaa agaattgaaa
    28381 aaatagtcaa caatttgtat aatagaaaca attatttata caatggatac gaaaaatcgt
    28441 ttgaaaaagc ttttaaaaag cgaacatttc cgtaaaaaaa cgtataactg cacggatttt
    28501 gggacaatta tcaaacagat atttgtcccg attcccgttt taccgaaaaa agaaaggggg
    28561 atcgattcct gtgacaaaag aagaaatttt aacgggaccc ggtgtcataa agctcgttag
    28621 tcaatacatg ggtcccgaac atgtagcatt tgttgagaaa gcatgcgaat acgcgactgc
    28681 tgcacatgac ggacagttta gaaaatcagg cgaaccctac attattcatc ctatccaagt
    28741 cgcaggtata ttagcagatt taaaaatgga tccccataca gtggctacag gcttcttaca
    28801 tgatgttgtt gaagatacag aaatcacttt agaagatctg agagaagaat ttggcgatga
    28861 cgttgctatg ttagtggacg gcgtaaccaa attagggaaa ataaaatata aatcccacga
    28921 agagcagcta gcagaaaacc accgaaagat gctgctagca atggctcaag atttacgagt
    28981 tatcatggtc aaattagctg atagactaca taacatgcgt acgttgaagc acctgcgaga
    29041 agataagcaa agaaggatcg ctcaggaaac tttagaaatc tatgcgcctc ttgcacatcg
    29101 tctagggatc agccggatca aatgggaatt agaagataca gcacttcgtt atctaaatcc
    29161 aaaacagtat taccgtatcg tccacttgat gcagacgaag agagaagaac gtgaaaaata
    29221 cgtaagtggt actgttgaag atattcgaat agcgacagaa gagctgggga tttttgcaga
    29281 aatctatgga cggccaaaac acatttattc aatctatcgt aaaatgaaag atcagaaaaa
    29341 gcagttcaac gaaatttatg acttgctagc tattcgagtg atcgtagatt caatcaagga
    29401 ttgttatgct gtattaggag caatccacac aaaatggaaa ccaatgcccg gcagatttaa
    29461 ggattatatt gccatgccaa aagctaatat gtaccaatct ttgcatacta ccgtgatcgg
    29521 tccggcagga aatccggtag aaattcaaat ccgaacacag gaaatgcatg aaatcgctga
    29581 attcggggtt gctgcacact gggcctataa agaaggaaaa aatgaaaaag tagaaccaga
    29641 tggtatgacg aaacaattaa gctggttcca tgagatactc gaacttcaag acgaaagcta
    29701 tgatgcttct gaatttatgg aaggcgtaaa aggagatatc tttagtgata aagtctacgt
    29761 cttcacacca aaaggagacg ttactgagtt accaaaagga tccggaccat tggactttgc
    29821 atacagtatc catacagata tcggtaacaa aaccactggt gcaaaagtaa atggcaaaat
    29881 ggtgcagctt gattacaaat tgaaaaacgg agatatcatt gagatcatga cttctccaaa
    29941 ttcatttggc ccaagtcgcg actggttgaa attagttgct actagcaaag caagaaataa
    30001 gatcaaacgt ttcttcaaag cccaagatcg agaagaaaat gtgatcaaag gccacgaatc
    30061 cgtggtcaaa tgtattacag atctaggatt tacgcctaaa gatattttga cgaagaacaa
    30121 actgcaagaa gcactcgatc gttttaatta tcaaacagaa gatgatctct atgcagctgt
    30181 agggtatgga gaagttagcc ccttgacgat ggccaatcgt ctgactgaaa aagaacgtaa
    30241 agaacaaaaa atcgagcagc aaaagcaaga agcagaagaa atcatgaatc agccgaaaaa
    30301 agaacctgac aaaatgaaag tacgtcatga aggtggcgtt gtcattcaag gggtagaaaa
    30361 cttactaatt cgtatcagtc gctgctgtaa tccgattcct ggtgatgata tcgttggtta
    30421 tatcactaaa ggcagaggga tatccattca tcgtcgagat tgtccgaatg ttcagcctga
    30481 caaaccaaat gtagcagaac gtttgattga agtcgaatgg gaagatacat cgaatacacg
    30541 aaaagagtat gatgcagatt tggaaattta cggctataat cgttcaggct tattgaatga
    30601 tgtacttcaa acagtcaatg cgctaacgaa aaatctcaac agcgttgaag cacggacgaa
    30661 taaagataaa atggcgacga tccatttgac ggttggtatc cagaatttat cccacctaaa
    30721 gagtatcgtg gataaaatca aagcagtacc tgatgtctac agtgtacgcc ggacgaatgg
    30781 ataggaggac agatatgaga gcagttgttc aacgggtaag taaagctgct gtgtcagtcg
    30841 atcagcaaga aattggaaaa attggctacg gcctacttgt tttattaggc attcatgaaa
    30901 ccgatacgca aaaagatgtg gactatctgg taaaaaaaat tgcacaaatg cgtatttttg
    30961 aagatgaaca aggaaaaatg aatattagtg tagaagatgt gggaggacaa attttaagta
    31021 tctctcaatt cacactgttt gcggatacga aaaaaggaaa tcgaccaagc tttgtggaag
    31081 ccgcacgacc agaaacagct attcctttat acgaatcatt caatgaagga atcagaagtc
    31141 gcggcatcac agtcgctacc ggacaattcg gagcagatat gtcggtttct ctcgtcaatg
    31201 atggacctgt aacgatcatt attgattcta aaaacaaata aagtaatctg gttttcacta
    31261 gtaaaaacta tgaaacacgt cgcttaagtt agactgataa actctaactt aagtggcttt
    31321 tttgtctgca aaaagcgctt cttttgtcag cagtcggaaa aaaagttaag cttaaattat
    31381 acaagttatt ttttatagga ggttgctgaa gatgaaagaa gtaccagtag tagatatcaa
    31441 aatcacagat ttaaaaaaat tgtatcaaaa aggatacaac atgttggagg agttacgcca
    31501 tgaagcggac gctcccgtcg tcaaagcaaa gattttcaat aaagaagcta tcacgatcta
    31561 cggatcatca gcagcaaaag tattttatga tccaaggaat ttcaaacgaa aaggtgctat
    31621 gccaaaatta gtattgaaga cactttttgg tcaaggtgga gtacagactt tggatggagc
    31681 tgctcatcac catcggaaaa atatttttat ggatctaatg acaccggaaa gaatggaaga
    31741 ttatcatcgt atcttagata agaatctgac acaagcactt gaagcacagc atggtcaatt
    31801 tgaactattt gatttaagta agatggtttt ctttacatct atttgcgagt gggcaggtat
    31861 caatttatca gcgatttcta aagatgaggt cgaaaaatta gcagaatatc aaatctctat
    31921 gatcagcgga acgtttactt ctccgattga tcacataaaa ggtgtcgaga atcgcaaaaa
    31981 atcagaaaaa tgggcgcaag gcttgattga agaagctcga caaaatcctg tggctggaaa
    32041 agaaaatgtg gcattatatg cttttgccaa tgccacagat ttagatggtc agcttttacc
    32101 tctggaagtg gcagctgttg aattattgaa catcatccgt ccaacggttg ctctaactgt
    32161 gtgggcggca ttgatgggac atgcactttt cagtagacca gatttgtatc agcaattaaa
    32221 aaatgacttt tcgacgttac aagatccgtt tattcaagaa atgagacgct attatccttt
    32281 cttcccaatg ctacctgcga tttcgctgaa agaagtagaa gtagatggtt atcgtatacc
    32341 agaaggcagt tgggttattt tagatttata tggaacagac catgatgaac gaacagttga
    32401 agcgccagat tcctttatga tcaaacggta tgtagggaaa gcaaaagata tttcctataa
    32461 agaagaatat gagatgattg cacaaggcgg gggaaatttt cgacaaatgc atcgctgtgc
    32521 aggagaatgg atcactcttc atagtctacg tgtgttttcc gatcagctag taaataagtt
    32581 tgagttctct gtacctgaac aagattggac gattccattc aatcagtttc cgacttatcc
    32641 gaatagccga gcgctacttt acaagaatta gattagcata aacttatcga aaaaaaggaa
    32701 gatcaaacag tgctgatctt ccttttttca tattactata aattgataag gtcaattgat
    32761 caaaatccac gaaatgcttc atcaatggtt tgataatctt cattggataa atgcaaatct
    32821 aaagcttgga cattgtcaga tacttgctct ggacgcttag ctccaggtat aactacagac
    32881 actcgtggat tcttcatgta ccaagctaaa acaagttgcg caggagtggc ttggtatctt
    32941 ttagcaatgg ggcgaagtac atcgacagcg gttaaaattt ctctgaaacg ttctccttgg
    33001 aaatctggat ttctttttcg aggatctcct tcaccaaatt gtttttcctc accaagttca
    33061 tattttcctg tcagcaaacc ggaagcaaga ggaaaatagg gaacaaaact gattttgttt
    33121 ttttcaagat aagggaataa ttctttttca gcctgccgat ggatcaaact gtatttatct
    33181 tctactacat cgacatatcc atctgcattc gcttctttca attgttctaa cgtgaagttt
    33241 gacacaccga ctgctcggat ttttcctgct tcttttaatt cgtgcaatgt agcaactgat
    33301 tcattttttg gtgtgctttc atccggaaag tggatataaa aaatgtcgat gtaatcagtt
    33361 tgcagacgtt ttagtgcatc ttccaccgat tgtttgagaa actcaggaga attatcaaat
    33421 gttcgccctt tatttggtac atgagcagct tttgtagcga tgacgacacg tgagcgatca
    33481 tattcttttt ccttcagtac ctcgccaatc agttcttcgg aacggccgtt tccgtaagca
    33541 taagcggtat cgatcaaatt gatcccgcta ttcaaagctg tacgaactac ttgttttcct
    33601 gtctcatctt ccaaaccgtc aaaaagatta tgtcctccaa cggcatttgc tcctaatcca
    33661 agctgttctg cgtagacttg tgaatgtccg attcttattt ctgccatagc tgattcctcc
    33721 tttttgtcta gtctaaaaca aaataccaaa aagctgaact aaaacgtttt tgtgagggga
    33781 aatagtcaga tatctcttga ctttgagact ggtttttagt aggttataag taatatcgaa
    33841 tgtcaatgaa agagcgagta agtttttgaa actttacaag agaggatcat cgaggctgaa
    33901 aatgatctaa agtttcatta gcctaaagac actctggaga tggacgagtg aaaagccgtc
    33961 ccgcgtacga gcgttattcg taagaggaaa gagaaatctt tcaaaattag gtggtaccgt
    34021 gtatgaattt cattcaccct tgttcatgat gaacaagggt gattttttta tttacatagc
    34081 taagaaaggg agagaaaact atgagtttcc aaagaccaaa aggaacaaat gatattttgc
    34141 caggcgaatc agaaaaatgg cagtttgtcg aagaaacagc acgtttatta ttcagagatt
    34201 atcagtataa tgaaatgcgt acaccgattt ttgaacacta tgaagtgata tcccgaagtg
    34261 taggggatac aacggatatc gtttcaaaag agatgtatga tttttatgac aaaggggaac
    34321 gccatgtgac gcttcgacca gaaggtacag caccaatcgt tcgttctttt gttgagcata
    34381 aattgttcgg cccagagtat gcgaaaccgt tcaaaacttt ttatatggga ccaatgtttc
    34441 gctacgaacg ccctcaaaaa ggacggttac gtcaattcca tcaaatcgga gtagaagcat
    34501 ttggatcaga aaaccctgca actgatgtgg agagcatggt gatggcactt gatttcttca
    34561 aacagttggg gatcacgcaa atccggctag tcatcaattc attaggtgat aaagaaacgc
    34621 gtcaagccta tcgtcaagca ttgatcgact atcttttacc ttttgaatca gagctgagtg
    34681 aagactctaa acgccgcctt catgaaaatc cattacgcgt attggacagt aaagacaaac
    34741 gagatcaaaa atttgtggca gaagctccat ctatcttaga ttatctaagc gagcctgctc
    34801 aaaaacattt tgataccgtt gtttccatgc tggatgcatt ggctgttcct tatgaaatcg
    34861 acagtaatat ggttcgcgga ctagattatt acacacatac gatttttgaa atcatgagtg
    34921 acgcaccaaa aatgggggca caagcaacga tttgcgcagg cggacgttat gacaatctag
    34981 tggaagaact aggtggtcct tcaacacctg gcttcggatt tgcaatgggt atcgagcgat
    35041 tactcttgac tatggaggca gaggaagtag ttatccctgc atttaatgag ttggatgctt
    35101 acgtggtcgc actcggagac gaaacaaata tcgaagcatt gaaagtggtc caagcgattc
    35161 gaaactttgg tttttcagct gaccgcgact tcatgaaccg taaagcaaaa gcgcaattta
    35221 agacagcaga taaagcgaat gccaaattag tattggtgat cggtgaagat gagcttgcta
    35281 gtcaaacgat taatgtcaaa gcaatggcaa gccgaaaaga aaaaagtttt ccattagaag
    35341 agatatatga acattttgat gaagtctatg atgaaatgac tttattcgag taggaggaaa
    35401 aaaataaatg gcacaaagaa cgatctattg cggattagtt tccgaacaac aattagaaca
    35461 aacagttaca ttaaaaggat gggtacaaaa aagacgagac cttgggggag taatctttat
    35521 cgacctacgt gatcgtgaag gaatcgttca aatcgtcttt aatcctgaaa tcaacaaaga
    35581 agcatgggaa atcgcggata aatgtcgcag tgaatacgtg atcgaagtga ctggggaagt
    35641 tagaagacgc ggagaaaatg cagtaaatcc taaaatggcc actggtgaat tcgaagtgat
    35701 tgcaagtgaa atcgtgattt tgaatcaagc aaaaaccccg ccatttcaaa ttgaagatga
    35761 tcaagcaatc agtgacgaaa tacgtttgaa atatcgttat ttggatctgc gtcgtccaaa
    35821 aatgactaaa aacatcaaat taagaaatgc gacaacaaaa gcgattcgtc attatttaga
    35881 tgataatgac tttctcgata tcgaaacacc ttacctaggg aaatctacac cagaaggcgc
    35941 aagagactac ctagttcctt cacgtgtcca tgctggacat ttctatgcgt tgccgcaatc
    36001 tcctcaattg tttaaacaat tgctgatgaa tgctggattc gaccgttatt atcaaatcgt
    36061 tcgttgtttc cgcgatgaag atttacgtgg agaccgtcag cctgaattta cgcaagtaga
    36121 tattgaaaca acgttcttaa cagcagaaga aatccaaact tacacagagg gattgattgc
    36181 gaaggtaatg aaagaagttc gcggtatcga agtcacattg ccatttcctc gtatgaccta
    36241 tgatgaagca atggctcgct acggcagcga caaaccggat acacgttttg atatggaatt
    36301 gatcgatcta agcgatacag tcaaagaagt agaattcaaa gtcttccaaa tggctcttga
    36361 aaacggcggt gtcgtgaaag cattgaatgc taaaggcgct gcagatcgct actcacgaaa
    36421 agacatggat cagttaggtc aatatgtagg acaattcgga gctaaggggc ttgcatggct
    36481 gaaagtagaa gaagacggat tgaaaggtcc aattgctaaa ttcatgggag aagcaacaga
    36541 agcaatcatt aaagcaacag atgcaaaacc aggtgacttg ctgatgtttg gcgcagataa
    36601 aagtgaaatc gtcgctgctg cattaggtgc tatccggaca cgtttaggaa aagaattagg
    36661 actaattgat gaatcgaaat tcaatttctt atgggtaact gattggccac aatttgaatt
    36721 ttcagaagaa gaaggtcgat atgtctctgc tcaccatcca ttcacgatgc caaaagaaga
    36781 agatatcccg ttattggctg aagaccctgc aaaagtctat gcagaagctt atgatatcgt
    36841 cttgaacggt tatgaattag gcggaggttc tatccgtatt catactcgtg agcttcaaga
    36901 aaaagtacta gaaactctag gctttacaaa agaacaaatg gaagaacaat ttggtttctt
    36961 gttaacagct ttagattacg gattccctcc acatggcggt attgcattag gattggaccg
    37021 ttttgcaatg ttattagccg gagaagataa tattcgtgaa gtcattgcct ttcctaaaaa
    37081 cggaaaagca gcagatgtga tgagtgatgc accaagtatg gtttcgcctt tacaattgtt
    37141 cgaattgaat attgatgtaa cgaatataga agaatagccg ataaaaaggt aaaaaggatt
    37201 tgtcgaatac gcttcttttg acaaccttga tttaattgaa aaacacttta taatgaagcc
    37261 ataacatata aagcggggct tttccatgaa aaaaacattc gaaaattggg cttatcatga
    37321 ttacacgacc aagctttcgg tatctatcgt ttatgcgttg atcgcatcag tcgcattgaa
    37381 tttcttttat cagccaggac atatttattc aagtggtatt actggtctag ctcagatttt
    37441 gacgactttg tcaaaatcac tgataggatt cgaagtaccg gtatcaacaa ctttgtattt
    37501 actgaatata cctttgtttg tattggcgtg gttaaaaatc gggaagaagt ttacaatttt
    37561 cacttttatc acagtcactt tgacctccct ttgtatccat attttaccgg aaacagtcct
    37621 atctaccgat ccaatcatct gtgcgttgtt tgggggggct gtgatgggac taggtattgg
    37681 gctagcgttg agaaacggcc tatcatcggg cggtctagat atcgtgagta tcacgattcg
    37741 taaaaaaaca ggaaagtcga ttggttctat ttcgatctac ttcaatgcat tgatcgtttt
    37801 tgttgccgga attctatttg gctggcaata tatgttttac agtacgttgt ctattttcgt
    37861 cagtggaaaa gtaactgatg tcgtttatac taaacaaaag aagatgcagg taatgatcgt
    37921 aacgaaacat ccagaaaaag tagtaaatga aatccaaaac tgtatgcgta gagggatcac
    37981 gatcatcaat gaagcagaag gtgcttataa gcatgataag cagacagtat tgatgacgat
    38041 cgttacacga tacgaactgc ctctacttag agaagcaatg aaagcttctg atccacaagc
    38101 ttttgtaagt attgcagaaa acgtccagat acttggtcgt ttctatgagg atggcattta
    38161 gatcgagttt gacaaaaatt catgaaatac tgctgtagac agacgttcgt ttgtctgcag
    38221 tttttttcta ccatagtaga atcagcaaag aaaaactgct tttttatttg aaattttctt
    38281 aaagttgaaa aaaagcatgt ttattttttt aaatttatgg gatgatagaa aaaaatagta
    38341 gagaaaggac acgattctac ttgctatttt cacttaatac ccgtatatta cgaatgaaaa
    38401 aagtttatga aaagttggaa tattgaatgg aaaaacaaaa aaggttaaaa aacagccgct
    38461 acatcactgg gtttgatggg ctccgttcat tagctgtggt gggtgttatc ttctatcacc
    38521 ttctgccaac tagtatgaaa ggcggctatt tgggcgttcc gatttttttc gtcgtatctg
    38581 gttatttgat cacggatctg ctgcggcagg aatgggaaca aaatggaaaa atcaatattt
    38641 ggcaatttta tgtcagaaga atgaagagac tctatccggc acttgttttt atgcttgtga
    38701 cagcttcggc ttatatcact ttgtttcagc gagggctgtt aaacaatctt cgaggagttg
    38761 tggtcagcag cttactgtat gtcaacaact ggtggcaaat caataacggt ttatcttatt
    38821 tcgatcgatt tgctaatgag tcaccgttta cgcatatctg gtctttagca gtagaaggtc
    38881 aaaactattt ggtttggccg atcttgtttg tattgttgat ggtatttgtt cgtaaaagaa
    38941 aatggatctt ttatactgtg ttaggtacat ccttcctctc cgccatcttg atgatgatct
    39001 tgtatacacc aggaggcgat ccaacccgtg tttattacgg taccgacact cgtctgtttt
    39061 ctatatggtt gggaagtgcg cttgcgtttg tttggccaag tacgcgttta aagaaaaaca
    39121 tcccgaaaca agcgaaaaat attctgaata tcgctggagg aatctcgctt ttggcactga
    39181 ttctttgctt cttcttttta gatgatcatt acaatttcat ctattacgga ggaatgctcc
    39241 tagtgagtct gttatgcgta gtgctagttg cagtgacagc acaccccgga gcaagtttga
    39301 atcgatggct gacaaatcca gtatttactt ggattggcaa acgaagctat ggcatttatc
    39361 tgtatcagtt tccagttatg attttttatg aagcaaaggt gactgatatt gccgatcatg
    39421 ttttgttgca tactttagtg gaaatcacat tgattctaat catcagtgaa ctttcttatc
    39481 gatatgtgga gcgtccgcta gcacgttttg attatggtcg tacattccaa gtgatgaaag
    39541 gatggttcag caaaccgatc atcagtaaga gcaaaccatg ggttgttcca ggcacattac
    39601 ttgtagcaat tgcactcgtt ggtttcgtta cggcaccgaa aaacgctgta acagccgatc
    39661 aaaaacaact gcaggcaaaa atcgctgaaa gtaaaaaatt agcagaagaa accagaaaca
    39721 attcagaaaa caccactagt tcggttgacc aagcgatcct tgataagtac gagttgtcca
    39781 aggcacaagg gaataaggca caaaaattag aactaacggc atttggtgat tcagttatgt
    39841 tggatgctgc aacggatcta caagaacttt atccaaaagt agtggtagat ggagatgtcg
    39901 gacgacaatt atatgccagt gtaccgtaca ttgaaaaatt aaaggaacaa gatttgttga
    39961 aagacactgt tctgattgga ctaggaacga atggctcatt ctcggaagct cagtttgata
    40021 gtgtgatgga tgcaatcggt gatcgaaaag tatactgggt caatgtccga gtaccgacga
    40081 agcgctggca aaatgacgtg aatgcgatgc ttgaacagat gacgaaaaaa tacgacaata
    40141 tgacgctgat tgattggtac agctacagta atgaccatag tgactggttt tatgatgatc
    40201 aagtccatcc aaatcctgaa gggatgacac attacattca cttagtcagc gaagccttgc
    40261 taggaggaag ttcaagtacg aacagcagtg agacaaacgt gacagaatga tagctacaaa
    40321 aaacttcagg caaaaacatt tgtttttgcc tgaagttttt ttatatttac cgtgttttta
    40381 acattcatat agattcagga agcaaaacgg ttgagtgtgt ctgtaaataa cgttcttttt
    40441 gttttcgggt gagttttttg aatgcagctt ctgcttttgt tgcttcgctt cttgaagaaa
    40501 atacttctgc atggatcatc ttaacaggtc ttcttttttg aagacgagta tatttagcac
    40561 cagtaccatt gttatgttcc tctaatcgtc tagaaagatc ggttgtatat ccaccataaa
    40621 atgtctgatc tttacagaat aatacgtaaa aataatgttc atttgccata gagcatctca
    40681 cgaatctctg cagtgtattc tccatccata ttgtaagtga tcaatggtgg agcgactttg
    40741 aatccgtcta atttgccttc tttgattcct tcaactaaca aaatatttgc ctcttttcca
    40801 ggttttgggt aaacaaattg gatcctttta ggtgcaattc gatagtcttc catgacctga
    40861 atgatttcta agaaccgatc aggacgatgg accatagtaa agcgtccatt tgtttttagt
    40921 agcttacttg aaatatcgat cacttcttct aacgtcgtat gaatctcatg acgggcgatt
    40981 gccaggtgtt tattagggtt cgttttattt gttggcagcc ctttgaaata aggtggatta
    41041 cagacgacta agtcacaaga attatgtcga acgacagata agctgtcttt cagatcgatg
    41101 gtgtggacag tcatttgttc atttaaatgg ttaagcgcga tactacgtct tgccatgtct
    41161 gctagacgag gttgaagttc gatagcgtcg atagccgcct gggttcgttt acttaaaaaa
    41221 agtccaactg cgccatttcc tgcacaaagg tcaacaatct tccctttttt aggtacacgg
    41281 gaaaagtgag caagcagcac agcatctaaa gaaaaagaaa acactttgga actttggata
    41341 atcttgatat cttctgcgta taattggtcg attcgttcgt cttcgtgaag catggattcc
    41401 ctccaaaatg aaaaaaatag tcgtctgtat tatactattt ttattaaaaa gaaccaaggt
    41461 ggcttgtcaa acctctattt tttatgcata ctatgaccat aagaagggaa aggaagagac
    41521 tatgttttat cgatttatgc gcggggtagt tcgagtacta ttagccttag ttaatggaaa
    41581 tgcccgctat gagaataaag aagttttacc gcaagatgag aattacatac tagttgctcc
    41641 ccatcgtact tggtgggacc cattatatct ggcggtagct gcagcaccca aggaattcag
    41701 ctttatggca aaagaagaac tgttcaaaaa tccagttctg cgatttatat taactcgatc
    41761 caatgctttt ccagttaaaa gagaaaatcc aggaccaagc gtcattaaaa cacctgtaaa
    41821 gattttgaaa tcgacaaatt tgagtttgat catgtttccc agtgggacaa gacatgctac
    41881 agaacttaaa ggcggaatgg cattgatttc cagaatgtct aaagcgaaaa tcgtgccagc
    41941 tgtatatcaa ggtccgttga cacttgggga tctgttcaag cgtcagagag tgacagtgcg
    42001 tttcggcgaa ccaatcgact tatcagatat caagaaaacg gataaagaag gtatggcgga
    42061 gatcgaacga cgaacacaag cagcttttga caaattggac aaagaagtga accctgattt
    42121 caaatatgaa gtcaaaaaaa aataaaatga taagtttatg cgtgggcggt cttttgaatc
    42181 tactgtcctg ttcagttcag actttttcaa tgaaaacgag ggagagacgt caagtctgcc
    42241 ctctttttgc gttccaatat gtaaaataga agacaggtgt catgtaatgg atcaaagtgt
    42301 tataataatt agaaggacat tttttgaggt gattgaatgc gttttttaca tacagccgat
    42361 tggcatatcg gaaaaaaact acaaggttat gacttgattg aagaccagcg tcatgtacta
    42421 tcttctatac tagatatcgc gaaagaagaa aaagtagagg caatcgtgat tgccggagac
    42481 ttatatgata ggagtgtccc agctgttgat gccgtagaat tatacaatga attgatggtc
    42541 gattggaatt taaaggaaaa gatcccgatt tttgctatat caggaaatca cgatagcagt
    42601 acgagacttt cagcgggtac gccatggttc agttattcga actatttttt gcgaacacag
    42661 ctagcagaag catttgaacc agtcgagttt aaaaacgtac aatttttctt tcttccttac
    42721 tttgaaccaa ttgcagcgag attatatttt gaagatgaca atatccgaac gatcaagcaa
    42781 gcggtagcca aggtgataga taaaatgcag gagctatttg attcagaaaa aaaacaagta
    42841 ttgattagcc atttttttgt aatgggaagt gtaaaatctg attcggaaac gaaaatcgaa
    42901 gtaggcgggt tggattcgat ccctggtgaa ctacttacac cgtttgatta tgtagcacta
    42961 ggacatttgc ataatcaagc tgctttgaga caggataatg ctcgctatag cggttcccca
    43021 ttgaaattct ctttatctga gatcaaccaa aaaaaaggcg tatggatcgt tgacctgaca
    43081 acaggcctgg agttgaattt tagggagatc gaacccctgc atgatattga agaaataaca
    43141 gcgagtttca aagaattgct ggatcctgcc ttttatcagt ccgtcaatcg ggagaattac
    43201 atctatatct atctcactga tagaacgatc atcccgaata tgatgaacca gcttcggcaa
    43261 atatatcctc gtattttggg gattgaacgg gcaaacggtc gagcagaaca aacaaagcgc
    43321 aaacgaaaag tactagaaat gaaagatcct caaaaattag cagaagcatt ttttagtgaa
    43381 atgacggaag agcagctaac tgcaaaccaa gaaaaatggc tcggagaaac gttgcaagaa
    43441 atcatggaaa tggaatgatc aagaatgaaa ccgaaaaaat tacaacttaa aaattttggt
    43501 ccttttatca atgagaccgt agatttttca cgtttgacag aagcaccgct ttttttgatc
    43561 agcgggaaga caggagctgg caaaacgacg atttttgatg ggatcacctt cgcacttttt
    43621 ggtgaaactt ccggaagact tcgctctgga aaagagatgc gttctttgtt tgctacacca
    43681 gacgaagaaa caagcgtgac tttttctttt gaacatcagc agatgagcta tgaaatcgaa
    43741 cgaaaaccag aacaagtttt agcaaaacga aaaggaaacg gaacaaaaaa acaggcagcc
    43801 aaagtaacgt tgaccatttt tgatgggaaa ggaaaagaaa taaaacaaat cacaaaacgt
    43861 acagaagcag atcagttgat cagagaactg atgaatctcg atgcgaaaca gttttctcaa
    43921 atcgttttac taccccaagg agaattccgt aattttttgg tttcatcaag cagtgataaa
    43981 gagactgttt tacgtcattt attcggtact caatttttcc aacagttcaa tgagcgatta
    44041 aaagaaaaag caaaacaaca gcaacaaaaa ttggatcatt tagagcaaga gctgcatctt
    44101 ctgcaaaaaa ggtttgtttt agtcaaagaa gaggaaccag cagcagggtt tgaagccgtt
    44161 ttagcccaat ggacaaacca tcaggcaata ctcgaaaaac aaatcacgga agaaaaagaa
    44221 gctctaacgg aacttcagga aaaacaaaag caactagaaa attcctacta tcgttttgaa
    44281 agcttgaaaa aaagttatga agaaaaaacg atattaataa acaagcaaaa ggaattagct
    44341 gctcaaaaag acgaaataga ggaaaagaaa cgatggattg ggtattatga atttacgcaa
    44401 cgattagtcg aaccaatcca gcgcctacaa gaaataaaag ttgaaaaaat agaactgtca
    44461 caaaaaagta gtgagcaaac aaagaaacta gcgatgacta cgcagaatta tgaagagtgg
    44521 caaaaaaatg aagcaaaccg cgtacgccga caaacaaaaa tcaaagaagc tgctcaaaaa
    44581 ctgcaaaaca aacagatgat ggtgccaatt gtcgaatcct tacgtagtaa aaaacaagac
    44641 gcagaacaat taacaatttc attggataaa gaagaattga atgccgagaa gctgtatcaa
    44701 aatctcgcta tctatcagga aagaactcga attcttcaag aaaatttact ggatcaggaa
    44761 aaactgcaag aagatcgtct tgcttttgga cagttaaaat atctagatga aaagcagcaa
    44821 gaaatagaaa aacaaaaagc aaaagacact gggtcgctcg cacagataag agaagaactc
    44881 aaaaaagcaa ctgaagaaat aacaaaaaat agtcagctat tgaaagaaca gacagaacaa
    44941 gcagaacagc tgaaaagcca atgggcaaag cagcaaattg ctcgtcttca gctttttttg
    45001 cgtccagggg agccatgtct tgtttgtgga tcaacggaac accccataca acaattaaaa
    45061 cacgaacagc caaataagga agatatctta aaagtagaac aacaactggc agaagctgag
    45121 tctgctgtag aaagaactca gcaaatcatt gccaaaagtg aagcacatga aaataatctt
    45181 caacaacaat tatcagattt agaacaaaaa atcatggcaa cgaatataca attggctgaa
    45241 caagaagaac acttgcaaca ggctttgaaa aaggcatttc ctgagataac agaacaaaaa
    45301 acaaaaacaa ctcttgtagt aatcgaacaa tctttagata gacgaaaaag caagatggcc
    45361 aatgcaagaa tagagctgga agaaataaga caacaagcag aaaagctcga acaacaatta
    45421 gctgtctaca aacagcagca gacagaaaaa aataatcagc tccagcaact gcaaggagaa
    45481 atcaaaagtt tagaacaaca attgcaagat acggatagta actgtttaga agaggagata
    45541 cgacgacttg aaaaacagtt agaagaagac tcgaatcaac tagctgttga gcagaaagca
    45601 ggagtgcaat tgttgcaaga tcttcatacg cttcggacac agcttgatct tcaacatgaa
    45661 caaatagaaa aaatagatga aaaaatagaa aaggaacaag aaagaatagc taaaaagtta
    45721 gaaagccagt cgtatttttc aacaattgaa gaaatctgtg catttgcaca acagcaagca
    45781 caatataaag aaaaccaacg tacagttact tcttatcatg aagagcaatt agtgacagct
    45841 gaccggctgc gtcaattaga aaatatccag ctaactgaga cattcccaga tacagaagag
    45901 atcagccaac agttagaaga aataagacgt atgatcgaaa taaagcaaga aaagatatac
    45961 ggtttacaag aacaaaaaat tagtaatcaa aaaatctacg atgaatgtct ttcgatttat
    46021 cacacaagcc agacgaaatt agatgagctt acgcaattac agcagctttc gcaaacattc
    46081 aacggagaaa atcctaagaa gactagtttg gaacggtatg tgcttcaggt ttatcttcaa
    46141 gaagtgttac aagtagcaaa tgatcatctc caacgtttaa cgaaaaatcg ttatcaattt
    46201 gagctagcgg ataccattgg cagttatcga gggaaaaccg gattagaaat caatatctat
    46261 gatgatcagg caggtatgtc tcgaagtgcg cataccctgt ctggcggtga gagttttatt
    46321 gcagcacttt ctttagcatt agctcttgcc gatgtgatcc agacacaagc tggtggtgta
    46381 gcaatcgatg cattgtttat tgatgaagga tttgggtcat tagatgaaga agctttggaa
    46441 atggcaatgg aagctttaga aacgatcgaa agtgaaggaa gaatgatcgg catcattagt
    46501 catgtaagag aattgaaaga acgagtatta caacaaataa gaattgatac agaaggtagc
    46561 ggacaaagca aaatacgcta tcaacttgga taaaaaggga gattggtaac tatgaaatgg
    46621 agtattccag aaaaagtcat tgcacgtgga cgtcaatact tgcaagaagg ccgagtgtta
    46681 tcggttgtcc ctgaccaaga agaaaacgtg tggcatgcag aagtcttggg cagtgaattg
    46741 tatatggttg atttggatgg tacagcaaaa gagatagatt actgccaatg tccctattgg
    46801 gaggaacata aatattgtaa gcatacagtt gctgtggaac tttatttacg gtcgaaaaat
    46861 ctatctcgta tcatgaaaaa agaccagcca atgctagaac cggtaaaagc aacaagtgag
    46921 ggagaaatca ttgcaaaagg tttttctcgt ctaaaaacac aaaacaatcc tcaagcggtg
    46981 caacctttga ttattgaata ccaagtagaa acgattgaga ctaatcagta tcacccagag
    47041 ctttctattt tggcacttta cttgcgtatc ggttcattgg catcaccgaa aaaaacatat
    47101 atcgtaaaaa acatctatga ttttttacaa gcttacacag aaaaagaaac ttacactgtt
    47161 aataaacagt accagtttcg tctagataaa cgggcgtttc aaaaagaaga tcaaaaaata
    47221 ctcacgcatc ttgcaggaag tgctcagacg catcaattat taggtacgaa cggcttacaa
    47281 gtcaaaggaa aactagataa aagatatctg cttctcccag tagaacaagc ccgcttcctg
    47341 ttagagcaga tgaatcagac tacacgtttg catctgcaga taaatgaaca aaacgatcaa
    47401 ggaatacaat tttccgatca aaacaaacct ttatcattca aagtggaaaa atttggagaa
    47461 gattatttgc tgcaagcatt gaatgatttt gattttttct ttatgcatta tcagtgggga
    47521 gttatccaag ggacatttta ttctctgacg aaatatcaac aaacggttta tcaaacttgg
    47581 aaacaattga ttcgccgatt ggagaaacca gaagtagtgt tcaagaaaaa agaattaccg
    47641 tctttgttta aagagattat ccctctgctg tcagagatcg gcgacgtagc tattgcccct
    47701 gcagtagaag aggaagtagc agatatcccc gtcgaattta ttttcttttt ccgaaaagca
    47761 aaaggaaaaa tccaagctcg agtcgatttc gtctatggtg aagtcatcta ttcgactgac
    47821 ccgaagcatg aggtctcgac agaacaggct tttcaggtgt tacgtgacac agtagaagag
    47881 aaacgagtac ttgatctatt cagaatgtat cgctaccaga aaaatgaaac tggttatgaa
    47941 cgtccattgc caagcgggga agaactatat gcctttttcc gcacagaact atctgtcttc
    48001 cgtcagtttg gtgaagtcag acttggaaga aaattgcgag agttgtattt agatgcgcat
    48061 aaacatcaac cgaaaatcga agtaaccgag accggttcat ggctcgatat tcgattcgat
    48121 gtgacaggga tagaggagca agaaatcgat catgtcctgc aaagcttgct gagaaatgat
    48181 gctttttata cattagaaaa tggtcaggtt ctttcttttg attcagaaga atttcaacaa
    48241 acaagccaag tgttgcaaca attacgggaa tcgatacgta cagaagaagg aacgatccat
    48301 gtacctaaaa atcaaggatt gatcattcaa aatcaattgg aaaaaagcaa tgctactttt
    48361 agtgaatcgt tccaaacgat ggtacaagat ctcattcatc cagaaagata ccaggcacag
    48421 cttcccaaag gattgaatgc aacgatgaga gattatcaaa agcaaggttt tcgatggttg
    48481 aagatgttag gtcattatca gtttggcggt attttagccg atgaaatggg acttgggaaa
    48541 acactacaga caattgcctt tttactttcg gaaaaagaag aacggaaaag cttctctgct
    48601 ttgatcgtag cgcctgcgag cttaatttac aactggcagg cagaggtgag aaaattcgcc
    48661 ccttctttaa gtatccaagt aatcaatggg aataaaaaag aacgtgaaga attactggct
    48721 aaagacacag atatccgagt gacttcttat gccagcttaa gacaggatct agctaattat
    48781 cagtcgcaaa aaattgatta tttgattctt gatgaggcac aaatggtcaa aaatagcagt
    48841 acgaaaactg cacaagcatt gagagaatta gctgtacctc agcggtttgc actgagtggg
    48901 acaccaatcg agaataattt agaagaattg tggtcattgt ttgcaacgat tatgccagga
    48961 tttttcccga ctaaaacaaa attcagggaa cttgccgcag aagaaattgc gcaaatgatt
    49021 cgaccgttta ttttacggcg agataaacaa actgtgttga aagacttgcc ggaaaaaaca
    49081 gagatgaatc tatattctgc acttacagaa gaacaaaaaa ctgtttattt agcttacttg
    49141 cgtcaaatgc gtgaagaaat cacatcaatg gattctgaag cattcaagaa aaaccggatc
    49201 gggatacttg ctggtctgac aagactgcga caaatttgct gcgatcctcg tctgtttatt
    49261 gaagattacc aaggcggttc cggcaagctc gaacaagtaa aagatctact ggtagctgcc
    49321 aaagagaaca aacgaagagt tttgctgttt tctcaattta cagggatgct gactatcttg
    49381 caagaagagt tagccgagct agggatctcg acattttatt taagaggcag tacgaaacca
    49441 caagatcggt taagtatggt agacgcattt aatgctggcg aaaaagatgt ttttctgatt
    49501 tccttaaagg ctgggggaac tggattgaat ctcacgggag cagatacggt catcttgtat
    49561 gacctttggt ggaatcctgc cattgaagaa caagctgctg gacgtgcaca tcggatcgga
    49621 caaaaaaatg tggtagaagt gtggcgtatg atctctgaag gaacgattga ggaacgcatg
    49681 gattcactcc agcaagaaaa acgcgaactg ttccaaaaag tgatccaagg taatgaagaa
    49741 cagttgacga aactgactga agaagacatc cgcatgattt taagtgtagg agaaatcgag
    49801 gagtaagaga ctcgaaaaag cgttcagtaa tgggtaagaa agcctttcta aagaagcgat
    49861 tgtttatttg atttaaaaaa tagcaaggag gctgggattc cattttatcc cagcttcctt
    49921 gctatcattt ttttatttct atttttaaag gttatacaaa gattgtcttc attgtctcta
    49981 acagctgatc aaaagaagaa atctcgatat cgcaaattgg agtctgttca attttgcgtt
    50041 ctcgatggtt gaaccaaagc gcttgccagt tagcactttt tgcacctaga acatcattgt
    50101 cgtaattatc gccaacgtat aaggtctctt ctggaagcat atgaaattct ttctcagcca
    50161 gctgaaaaat ctctttttct ggtttttgga aacctgttgc ttgagaaatg agcatattgc
    50221 gggaaggaat ccaattgttc aattgaagtt gcttcagttt ttttgtctga tggtcagtcg
    50281 gcccatttgt gataattcct aatgggacat tcttttcctt caagaaatcg agggtttttt
    50341 tgacctcctc atgcaagcag atattatcaa gttcttcctc gtaaattttt tgaaataaga
    50401 ggccatcttc ttccgtaata tgagggtagt ctaaatcttt tagtgattgg ctgatccgat
    50461 gtgctcgcat atattctagt gtccaatctc ctgccattac ttttgggaag ttttcatcac
    50521 tatgatggcg gaaccggata tatagtggat gcatatctgc atctgaaaca agcggaacga
    50581 ctcttttgac cgcgtttcga aatggctgct gctgatcata catcgtatca tcaacatcaa
    50641 acacaactga tttaatcaaa attttacacg cctttcggtt ttcattgatt ttttcacaaa
    50701 tccttgacta ttgtaacaga aaaaattgtc taaaaaagct gataaataaa ggtttttctt
    50761 actatatttc tatttccttg acagaataag gtttttatat ttcagttttt ttcactaaaa
    50821 atcgtgaaac acgtcaataa gtttgatgaa caaattaatc attatggtag aagtcgattg
    50881 atttatttta atgcaatctc tgtggaattt tcgaaag
//
LOCUS       DAFFSV010000006        50538 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFFSV010000006 DAFFSV010000000
VERSION     DAFFSV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.006"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            83..2680
                     /gene="adhE"
                     /locus_tag="LR110_000283"
                     /gene_synonym="adhC"
     CDS             83..2680
                     /gene="adhE"
                     /locus_tag="LR110_000283"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBL4821289.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2909..4015
                     /locus_tag="LR110_000284"
     CDS             2909..4015
                     /locus_tag="LR110_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL4821290.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4032..5060
                     /locus_tag="LR110_000285"
     CDS             4032..5060
                     /locus_tag="LR110_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBL4821291.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFQEAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5066..5545
                     /locus_tag="LR110_000286"
     CDS             5066..5545
                     /locus_tag="LR110_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBL4821292.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5561..6856
                     /locus_tag="LR110_000287"
     CDS             5561..6856
                     /locus_tag="LR110_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBL4821293.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6867..7691
                     /locus_tag="LR110_000288"
     CDS             6867..7691
                     /locus_tag="LR110_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL4821294.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIREELLTKFEQYLRYASS
                     FGASMVPSETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7856..8755
                     /locus_tag="LR110_000289"
     CDS             7856..8755
                     /locus_tag="LR110_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4821295.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8881..9750)
                     /locus_tag="LR110_000290"
     CDS             complement(8881..9750)
                     /locus_tag="LR110_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBL4821296.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9903..11363
                     /gene="rhaB"
                     /locus_tag="LR110_000291"
     CDS             9903..11363
                     /gene="rhaB"
                     /locus_tag="LR110_000291"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBL4821297.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11360..12616
                     /gene="rhaA"
                     /locus_tag="LR110_000292"
     CDS             11360..12616
                     /gene="rhaA"
                     /locus_tag="LR110_000292"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBL4821298.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12630..13475
                     /gene="rhaD"
                     /locus_tag="LR110_000293"
     CDS             12630..13475
                     /gene="rhaD"
                     /locus_tag="LR110_000293"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBL4821299.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13477..13788
                     /gene="rhaM"
                     /locus_tag="LR110_000294"
     CDS             13477..13788
                     /gene="rhaM"
                     /locus_tag="LR110_000294"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBL4821300.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13870..15021
                     /gene="fucO"
                     /locus_tag="LR110_000295"
     CDS             13870..15021
                     /gene="fucO"
                     /locus_tag="LR110_000295"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBL4821301.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15148..15906
                     /locus_tag="LR110_000296"
     CDS             15148..15906
                     /locus_tag="LR110_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBL4821302.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            16006..16650
                     /locus_tag="LR110_000297"
     CDS             16006..16650
                     /locus_tag="LR110_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBL4821303.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16657..17664
                     /locus_tag="LR110_000298"
     CDS             16657..17664
                     /locus_tag="LR110_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBL4821304.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVMEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17680..18522
                     /gene="kduI"
                     /locus_tag="LR110_000299"
     CDS             17680..18522
                     /gene="kduI"
                     /locus_tag="LR110_000299"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBL4821305.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18542..19345
                     /locus_tag="LR110_000300"
     CDS             18542..19345
                     /locus_tag="LR110_000300"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBL4821306.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19594..20658)
                     /locus_tag="LR110_000301"
     CDS             complement(19594..20658)
                     /locus_tag="LR110_000301"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBL4821307.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20655..21740)
                     /locus_tag="LR110_000302"
     CDS             complement(20655..21740)
                     /locus_tag="LR110_000302"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBL4821308.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21753..22730)
                     /gene="mvaD"
                     /locus_tag="LR110_000303"
     CDS             complement(21753..22730)
                     /gene="mvaD"
                     /locus_tag="LR110_000303"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBL4821309.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22723..23667)
                     /gene="mvk"
                     /locus_tag="LR110_000304"
     CDS             complement(22723..23667)
                     /gene="mvk"
                     /locus_tag="LR110_000304"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBL4821310.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23983..24642
                     /locus_tag="LR110_000305"
     CDS             23983..24642
                     /locus_tag="LR110_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL4821311.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24727..25632
                     /locus_tag="LR110_000306"
     CDS             24727..25632
                     /locus_tag="LR110_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL4821312.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25634..26266)
                     /locus_tag="LR110_000307"
     CDS             complement(25634..26266)
                     /locus_tag="LR110_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL4821313.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26417..26542
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26586..27110
                     /gene="infC"
                     /locus_tag="LR110_000308"
     CDS             26586..27110
                     /gene="infC"
                     /locus_tag="LR110_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBL4821314.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27182..27382
                     /gene="rpmI"
                     /locus_tag="LR110_000309"
     CDS             27182..27382
                     /gene="rpmI"
                     /locus_tag="LR110_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBL4821315.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27435..27794
                     /gene="rplT"
                     /locus_tag="LR110_000310"
     CDS             27435..27794
                     /gene="rplT"
                     /locus_tag="LR110_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBL4821316.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28046..29383
                     /locus_tag="LR110_000311"
     CDS             28046..29383
                     /locus_tag="LR110_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBL4821317.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPAALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29431..31560
                     /locus_tag="LR110_000312"
     CDS             29431..31560
                     /locus_tag="LR110_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBL4821318.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31535..32224
                     /locus_tag="LR110_000313"
     CDS             31535..32224
                     /locus_tag="LR110_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821319.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32632..33318)
                     /locus_tag="LR110_000314"
     CDS             complement(32632..33318)
                     /locus_tag="LR110_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4821320.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAMGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(33454..33648)
                     /locus_tag="LR110_000315"
     CDS             complement(33454..33648)
                     /locus_tag="LR110_000315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821321.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33698..34138)
                     /locus_tag="LR110_000316"
     CDS             complement(33698..34138)
                     /locus_tag="LR110_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBL4821322.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34142..34987)
                     /locus_tag="LR110_000317"
     CDS             complement(34142..34987)
                     /locus_tag="LR110_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBL4821323.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35136..36554
                     /gene="pepV"
                     /locus_tag="LR110_000318"
     CDS             35136..36554
                     /gene="pepV"
                     /locus_tag="LR110_000318"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBL4821324.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36611..37558)
                     /locus_tag="LR110_000319"
     CDS             complement(36611..37558)
                     /locus_tag="LR110_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL4821325.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37781..38131)
                     /locus_tag="LR110_000320"
     CDS             complement(37781..38131)
                     /locus_tag="LR110_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL4821326.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38331..39410
                     /gene="pepA"
                     /locus_tag="LR110_000321"
     CDS             38331..39410
                     /gene="pepA"
                     /locus_tag="LR110_000321"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBL4821327.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39554..39874
                     /locus_tag="LR110_000322"
     CDS             39554..39874
                     /locus_tag="LR110_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL4821328.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39896..40360
                     /locus_tag="LR110_000323"
     CDS             39896..40360
                     /locus_tag="LR110_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL4821329.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40565..41170
                     /locus_tag="LR110_000324"
     CDS             40565..41170
                     /locus_tag="LR110_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBL4821330.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEEFPK"
     gene            complement(41209..42498)
                     /locus_tag="LR110_000325"
     CDS             complement(41209..42498)
                     /locus_tag="LR110_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBL4821331.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42926..43405
                     /gene="rlmH"
                     /locus_tag="LR110_000326"
     CDS             42926..43405
                     /gene="rlmH"
                     /locus_tag="LR110_000326"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBL4821332.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            43490..43999
                     /locus_tag="LR110_000327"
     CDS             43490..43999
                     /locus_tag="LR110_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBL4821333.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            44099..44749
                     /locus_tag="LR110_000328"
     CDS             44099..44749
                     /locus_tag="LR110_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821334.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVAILNYYSHRQ"
     gene            45200..46138
                     /locus_tag="LR110_000329"
     CDS             45200..46138
                     /locus_tag="LR110_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL4821335.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            46151..47203
                     /locus_tag="LR110_000330"
     CDS             46151..47203
                     /locus_tag="LR110_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4821336.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            47469..48104
                     /locus_tag="LR110_000331"
     CDS             47469..48104
                     /locus_tag="LR110_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBL4821337.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            48337..48741
                     /locus_tag="LR110_000332"
     CDS             48337..48741
                     /locus_tag="LR110_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL4821338.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            48874..49917
                     /locus_tag="LR110_000333"
     CDS             48874..49917
                     /locus_tag="LR110_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821339.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMRTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            49932..50495
                     /locus_tag="LR110_000334"
     CDS             49932..50495
                     /locus_tag="LR110_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL4821340.1"
                     /translation="MITVVHKERQEKSSSDVQLWVYLGSISTKIQYGYTDSAKKKGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
BASE COUNT        16029 a         9031 c        10817 g        14661 t
ORIGIN      
        1 gtgtttacat agaaaaataa atattgtatg atatacatgt gaattattta acaaataacc
       61 atgacccatt aggaggataa tgatggctaa agtaatagaa aaagaaaaaa cgaagacaat
      121 tgacgtacaa gcaatgattg atgatttggc agaaaaagca aatgcagcat tgaaagaaat
      181 ggaaagcttt gatcaagaaa aagtagacca tatcgtgcat gaaatggcaa tggcagcttt
      241 ggatcaacat atgccattag cgaagatggc tgtagaggaa acaggccgcg gtgtttacga
      301 agataaagca atcaaaaata tgtatgcgtc cgaatatatt tggcataata tcaaacatga
      361 taaaacagtt ggtgtgatca atgaagatgt ccaaaaaggg ttgattgaaa ttgctgaacc
      421 agtcggtgtg gtttgcggag taactccaac aacgaatccg acttccacaa cgattttcaa
      481 atcaatgatc gcgttgaaaa caagaaatcc aatcgttttt gctttccatc caagtgccca
      541 aaaatcttct gcagaagcag cacgagtggt acgtgatgct gctatcgctg ccggagctcc
      601 tgaaaattgt atccaatgga tcgaacatcc ttcgatcgaa gcgacttcta tgctaatgaa
      661 ccatccaggt atcgcgatcg tcttagcgac aggtggagct ggaatggtca agtctgctta
      721 ctctacagga aaaccagctt taggtgtagg tccaggtaac gtaccagctt atattgaaaa
      781 aacagcaaaa attaaacgtg cagtgaacga tttgatcgtt tcgaaaacat ttgataatgg
      841 aatgatttgt gcttctgaac aagctgtgat cgtggataaa gaaatctatg cagcagtaaa
      901 agcagaattc caagcacacc aagtttatat cgtaaaacca gatgaattgc aaaaattaga
      961 agatgcagtg atgaacgaag gaaaatatgc ggtcaatcca tcaatcgttg gacattctgc
     1021 gatggaaatc gcaaaattgg caggaatcag tgtaccaaaa ggaacgaaaa tgctgattgc
     1081 tgaattggaa ggcgtaggcc cagattatcc gctatcaaga gaaaaacttt ctccagttct
     1141 tgccatgatc aaagctaaca acacggacca tgcgtttgat ttgtgtgaag gtatgttgaa
     1201 tcttggagga ttaggacata cagcagttat ccattcagaa aacgaagagt tgcatgtcaa
     1261 attcggtctt cgaatgaaag cttgtcgtat tttagtgaat acaccatctg cagaaggcgg
     1321 catcggtgat atctataacg aaatgattcc gtcattgact ttgggatgcg gttcttatgg
     1381 taagaactct gtttctcgta acgtctctgc tgtaaactta atcaatgtca aaactgtagc
     1441 gaaacggaga aataacatgc aatggttcaa attaccgcca aaaatcttct ttgaaaagaa
     1501 ctctctattg tacttagaga aaatggaaaa cgttgaacgt gtcatgatcg tttgtgaccc
     1561 tggtatggtc caattcggtt actgcgatac agttcgtgaa gtattggcac gacgtaaaaa
     1621 tgatgtgaag atcgaagtct tctcagatgt tgaaccgaac ccatcgacaa ataccgtata
     1681 cgcaggaacg aaactgatgg cagacttcaa gccagataca gtaatcgctc ttggaggcgg
     1741 ttctgcaatg gacgcagcta agggaatgtg gatgttctat gaacacccag acacatcttt
     1801 ctttggtgca aaacaaaaat tcttggatat ccgtaaacgg acatataaga tcgacaaacc
     1861 agaaaaaaca caattcgtat gtatcccgac aacatcaggg actggttctg aagtaacgcc
     1921 atttgcggtc atcactgaca gtgaaacaca tgtgaaatat ccgttagcag attatgcatt
     1981 gacgccagat gtcgcaatcg tcgatcctca attcgtcatg tcagttccag catctgtcac
     2041 agcagatacc gggatggatg tgttaacaca tgcaatcgaa tcctatgttt cagtcatggc
     2101 ttcagattat acacgtggat tgagcctaca agcaatcaag ttagtctttg actatttaga
     2161 aaaatcagta aaaacacctg atatggaatc acgtgaaaaa atgcacaacg cttctacaat
     2221 ggctggtatg gcgtttgcta acgcattcct aggtatttgt cactcagtcg cacataaaat
     2281 cggcggggaa tacgggatcc ctcatggacg gacaaatgcg attcttttac cgcatattat
     2341 ccgctataat gcaaaagatc cacaaaaaca tgcgatgttc cctaaatatg actatttccg
     2401 tgcagataca gattacgcag atatcgcaaa attcttaggt ctgaaaggcg aaacaacaga
     2461 ggaattagtc gaagctttag caacggctgt ttatgaatta ggaaaatctg tcggcatcaa
     2521 catgagtcta aaagctcaag gagtcacaca agaaacacta gatacaaccg tagaccgtat
     2581 ggctgaatta gcttacgaag accaatgtac gacagctaat ccgaaagaac cattgatctc
     2641 tgaattgaaa cagatcatca ttgatgctta caacggataa gcaaaagaaa gcgtagaaaa
     2701 aagataagaa aatagatgaa aaagaacttg gaaaacgttg tttctccaag ttcttttttt
     2761 aatttccaag taactgaaag ttacttttgt tatttaacat ttaggtcaat tttagacagt
     2821 ttttgttcaa aaagcgatat ttatagtgga cggtattact gctatattaa ggatataaaa
     2881 tgaaagcgtt taaaaaggtg gcgtaaagat gattattaaa gaagagattt ttgtacctaa
     2941 agaattagtg aaagaaaaaa ttgacctgct cgttgaaaac ctgacaacga tcaaagacga
     3001 taatggtgaa tttttgttgg actttgatgg actgaaagta gatgacaaaa gctggacagt
     3061 gtggaattgg ccacaaggtg tcggtctata tggtatttat aaaaattacc agatgacaaa
     3121 aaatcctcgc gcttatcaag tggtcaatga atggtttgag gatcgtatgg aagaaggcgc
     3181 tccgcccaaa aacgtcaata caatggcacc gctattgact atggcttact tgtatgaaga
     3241 cacgaaagat tcacgctatt tgccttattt agaacaatgg gcagaatggg tcatgaatga
     3301 catgcctcgg acaaatgaag acgggttaca gcatgccaca tatggaccgg aaaataagaa
     3361 tcagttgtgg gacgatacat tgatgatgac agtgcttcct ttggcaaaaa tcgggaaact
     3421 gttgaatcgc ccagaatatt tagaagaagc tcgttaccag tttatgatcc atatcaaata
     3481 cttgatggat aaacgaacag gattgtggta tcacggctgg acgtttgaag gaaatcataa
     3541 ctatgccgaa gcattttggg ctcgtggaaa ctgctggttg acgattgcta ttccagagat
     3601 cattgaaatc ttagaattat cgaaagaaga tgcattgcgc aaattattga tcgaaacact
     3661 tgaagcacaa gtgcgtgcat taaaaactta tcagtctgaa agcggtttat ggcatacgtt
     3721 gttggatgat ccttcttctt acgtagaatc ttctgcaact gcaggatttg cttacggtat
     3781 tttgaaagcg gtccataaac gttatttacc acaagaatat aaagaagtcg cttatcgtgc
     3841 tatccaagga ttgcttgaac aaatcgatga aaaaggagaa gttcaaaacg tttcaatcgg
     3901 gacaggaatg ggggacagtt tagattttta tcggaatatc ggaatcacag caatgcctta
     3961 tggacagtcg ttaacggtgc tttgtttgac agaattgctc gtctcttatt gttaaaagaa
     4021 aggaaaccca tatgaaaaaa acatcactgc tgtttgcagc aagttcgctt agcctaggtc
     4081 tgctaggtct aagcggctgc ggaaataatt ctagtgtggc aactgtcgca gatgcagacg
     4141 taaatgagat agttaccttg cgctttgctt atgcaagcaa cagccagcca gtaattgatg
     4201 caatgaatga atttgggcgt ttagtaaaag aaaaaacaga tggttccgta cagatccagt
     4261 atttcccaga tggacaactg ggtggagaac gtgaactgat cgaattgaca cagtcaggag
     4321 cagtagattt cacaaaagta agtgcctcag cgttggaaag tttctctaaa gattattcga
     4381 ttttttcagt tccttatcta ttcaatgatg aagaacattt ctttaaggtg atggataatg
     4441 aagaaataat ggagccaatc taccaatcga ccaaaaattt aggatttacc ggattgacct
     4501 attatgattc tggtcagcgc agcttttata tggtagacgg tccgatccat acgccggaag
     4561 atttgaaggg aaagaagatc cgtgtgatgc aaagcgaaac cgctatcaaa atggtagagc
     4621 ttttaggcgg ttcgcccgta cctatgggaa gtgatgaagt gtacacgtct cttcaatcca
     4681 atttgatcaa cggatcagaa aacaatgaat ttgtcttgta tacagctgga catggtggtg
     4741 tggctaaata ctattcctat gatgagcata cacgtgtgcc agatatcatc atcatgaatg
     4801 atgccatcaa agaacagcta actgatgagc aggaaaaagc cattgaagaa gcagcgaaag
     4861 aatcaacggt ttttgaaatt gaagcttttg caaaagcaat tgaagaagaa aaagctttag
     4921 cacaagaaga acatggagtc caattcaatg atgtagataa tacaccgttt caagaggcag
     4981 ttgccccact tcatgatata ttcaaaaacg atcctaccta caaagacctt tatcaaaaga
     5041 tccaagagca aggagcgtag ctgtgatgaa aatgatttta aacaaggtat tagagatcct
     5101 gggagctgtt ttgcttgttg ggatggtcgc tgttgtttta tggcaagtat tttccagaac
     5161 ggttttagga aatccaaata cggtcacgga agagcttgtt cgattcggtc ttgtctggtt
     5221 tgccatgttg tcttccgcgt atgttgtggg acaaaaagca catttagccg taacggtttt
     5281 aagtgaaaaa ttaactggca aaaaaagtaa aattttagaa gttgttgttc aaagtttatt
     5341 tctggtattt gctgcgatga tcatggtgta tggcggatgg aatgctgtca cattgacaat
     5401 ggggcagatc tcgccttctt taagtattcc tatgggatat gtttatctgt cagtacctgt
     5461 atcgggggta ctcatcatta tctatagcct gatcaatcta atcgatacat taagcaacaa
     5521 gccagcaatt caagaaaaaa tataaaaatg gaggaataaa atggcaatag aagccggatt
     5581 agtgttattt tttgtatttg ttgtgttgct tgtattaggg atgccgattg ctataagtat
     5641 tgcattctca tcgatgatga ctttattgtt agttataccg tttgatgttt ctgcttttag
     5701 ttcagctcaa aaaatggtgg gaagtatcaa tagtttttcg ctgatcgcta ttccgttttt
     5761 cgttctctcg ggcattatca tgaataatgg agggatagct aagaaactag tggattttgc
     5821 gatgttgttc gtcgggcgcg tgccgggggc tttggctcat acgaatgtgt tagggaatgc
     5881 tttatttggt tccatctcta gctcggccat tgccgcttct accgcgattg gcggagtgtt
     5941 gatccctcag caagtggaag aaggatatga ccggaagttt gcgacagcgg tcaatatcgc
     6001 gtcagctccg acaggtatgg tcattcctcc aagtactgct tttatcatgt attcacttgt
     6061 tgctggtggt gcttcgatct ctgctttgtt tatgggaggt tatctcgtag gcgctttgtg
     6121 ggcgctagga atcatgctag tagcattcat cgttgctaaa aagaataaat accctgttgt
     6181 cgaaaaagga cagatggctc atgctggaaa aataataaaa gaggcgattc ctagccttct
     6241 attgattgtc atcattatcg gtggtatctt gacggggata tttacagcag tagaagcttc
     6301 agcgatagct gtcatttact ccctgttgat tgctatgttt tactataaaa cggtaaaact
     6361 aaaagatata cctaatatgt tgtttcaatc agtagcaatg tcagggacga tcatgttctt
     6421 attggctact tcttccatga tgtctttcgc catggcgttt accggtattc ctcaggcaat
     6481 tagttcgttg attatgggcg taacggataa taaatttatt attctgttat tagtcaatct
     6541 tgtgttatta ctggtgggta tgtttatgga tgtggcgcct gctatcttga tttttacacc
     6601 aatattttta ccaatcgtca caagtgtagg ggttgatcct gtacattacg ggttgttttc
     6661 aatcatgaat ctgtgtgtag gctccatcac gccgccagta ggtacaggac tatatgtcgg
     6721 tgcaagtgta ggaggcgtaa aagcagaggc aatgctgaaa ccgcttcttc ctttttatgg
     6781 ggttatctta gtcgtcttgt ttttgattac ttacttcccg caaatcgtca tgtggctacc
     6841 caatatgctg agttaggaga atagttatgt tgaacattac aattagagga catgatcttt
     6901 ccgaagtcca aacgatagaa gatcttgcag aaaaaacaaa agaacaaggg attcatacac
     6961 tgcagctcgc cttaggtctt tcgtttcctg aaataccttc tggcgcaaat gagatcaatt
     7021 ctgggatggg aaattatgtc aaacgagttt tagaaaagca agaagtgacc gtaggaattt
     7081 taagttgcta tatcaatatg attcatccag atttaacgat acgagaagag ttgctgacaa
     7141 agttcgagca gtatcttcgc tatgcttctt cctttggtgc gtcgatggtt ccttcggaaa
     7201 ctggatgtgt tttgccggaa atccaatata cggaagaaaa tttcacagat gaagcatttg
     7261 ctgaagctgt gtccgttatc cgtcgcttgg taaaagctgg agaaaaatac caaatgatgg
     7321 tggggataga accaggtttg aatcatccgg tgtattcttt agcaagggta gagcagctga
     7381 tccaagcagt agattcggac tatctaggaa tcattttaga tccgaccaat ttgatcacaa
     7441 gtagtgatta tcaagaacaa gtccaattgg tagaagaagc ttttgaacgt tttggtgaaa
     7501 agatatgtgc ggttcatttg aaagattttc gtgtcgaaca ggaaaaaatc gtgccggtaa
     7561 atttgggaga cggtgtgatc gagtacacaa aaatcaaaga aatcatcatg aagaatcgtc
     7621 cgtacctgta tgtggttctt gaagaaacaa aagatgatgg gattcgttat ggaagaagtc
     7681 ttcttgaata atgtaaagaa gacgttggtt aactaatatt tagaatggag ggggcggggc
     7741 tagaagatgc cacgtctctt cttttttata agagtatata aaagcagctc tttttgatta
     7801 caaaaaacgt ggtataataa aattgtgcat aagtgaacaa aaggaggtgc ggcacatgct
     7861 ccaaaagaaa atcagcggtg aacaagtcaa tacgatggtt tctgatcagt atgaaatcta
     7921 tcatgtcaaa gatgtcgtca gccaaaataa aacgatctat catcatcatg atttctatga
     7981 gatccatgct acattaaagg gagaagcgac tttttatttg aacggacgac aatttacgat
     8041 caaagcagga agtgtgctgc tcatccattc caatgatttg catcgtatcg tccgccaatc
     8101 ttcagatgta ttcgagcgtg tctatatctt tgtcacccct tcttttctag aaagtcgttc
     8161 cacgccatgg tctaatctat cggcttgttt tcaaccaatg ggagagaaga ggagtcgtgt
     8221 attgcaaata gaacaggaag tattgaagca aaaattatca tttatcgacc atcctcctga
     8281 taaaaaaatt tacggtgaag acataaaata tgaacaagct ctggtagaat acttgatttt
     8341 tctcaatcaa gttgtacaaa aagaagagag tgcgcaagaa cagaatgctg tagttcctaa
     8401 tgagcgtgtg gaacaaatgt tacaatatat ctcaaaaaat ttatctcatc cgctttcttt
     8461 agaggaaatg gaaaagcagt tttttgtcag caaataccat atcacacgag aatttaaaaa
     8521 gtacactgga tttacgtttc atcagtatgt aatgaagaaa aagctgatct atgccaagca
     8581 cttgttgcga gaataccgca gctctagtgc tgtatatagt aaaagtggtt tttcttctta
     8641 tccgcatttt ctgaaagcat tcaagagaga gttcggcata acgcctaaag aatttttgaa
     8701 acgtgataca cagaaacagt tggttcatta tgaacatttt gaagatgaac aatgagaatg
     8761 atcactaaaa aataaccgca gtagctgctg ttgtttacat agaagcagaa actgcggtta
     8821 ttttattttt atctatatat ttcaaaatat tttgaaaaaa tgacgtacat attatatgaa
     8881 tcagctgaat ttacgaaagt ctctcggcga tagtcctgtt tttgctttga acaggcggct
     8941 aaagtaagca aaattctcaa tccctatttc ttcggaaatc attcgaaccg taaattctgt
     9001 ataccgaaga aggtctttag cgtaagaaag ttttttgctg atcaaataat cgattggtgg
     9061 agaaccgata aatttggaaa aatctttgct cagctgatat ttattcaata agaattgttg
     9121 ttctaattga tcaagtgtaa tcgtttcttt gaaatgttcg tctaagtatt gcttcagttt
     9181 gtaaacgtag gcgggaatat cctcttcttc gaaatctagc tgatactttt gcaaaatcag
     9241 ttcattcagc aactcgtgga tagctacaga ggactggaaa tcggtacggg cattcgtgcc
     9301 ttgttgcatg tggatcaatt ctgtgatcaa atggtggatc ttgttttctg atggatcccc
     9361 tgttgaatgg aaggtgttat ggccgtcttt tgtgaactcc tgataaaatg attggacgtt
     9421 ctgaccattc aaatggatcc aatccatttc ccatggttct tcgctgatcg tttgataacg
     9481 ctgatattct tggcagtcaa taaaaaataa gtctccagct tttagctgtt gctgattatc
     9541 attatatgta agcgaacctt tgccagacaa agtaaacttt atcaagtaag aaggtaaatg
     9601 tgctcgttca gtatagtaag ggattggtgc tttgaagtaa ccgatttctt ggatataaaa
     9661 aaataaattc ctgcttcttt gtgtaggcgt caagatcact ctttttgagt cttccgacca
     9721 tgagtggttg gctttttccc agtaatccat gtaaacactc ccaacttgtc aagataatgc
     9781 aattatatac caaaatagtg tgaagcaaaa tataagattc tgtattatga tagcattgtt
     9841 aataaatgat aacgctttat taataaagtg ctaatcaaca ataaagtaaa agaggtggag
     9901 aaatgaatcc aacatttgtt gccatcgata tcggtgcatc aagcggacga ctaatgaaaa
     9961 gtacgaggga tgcaaaaggt tctttgatct tggaagaagt acaccggttc aaaaacggct
    10021 ttcggaacaa agacggtcac gatcgctgga agatcgatta tcttatcaaa gagattttga
    10081 ttggatttga aaaattaaag cagacaggaa ttgatcgcct ttttgcgggg atcgatacat
    10141 gggcagtcga ttactgtctg attgatgaac aaggggatcg gataaatgat ccgattgctt
    10201 atcgggacaa gcgtaccaat ggagcagtag aacagttttc tgaaatctat tctctagata
    10261 agttgtatga gcagacaggg attcagatcc agcctttcaa taccttgttc caattgtttg
    10321 ctgaagaaaa agaaaatttg gcaaaagcac ataaattgtt actgatacct gattatttag
    10381 ggtatgtctt tactggtaac atggtgacgg agaaaactaa tgcatctact atgcagctgc
    10441 tgaatgcagg gacaaaagaa tgggaaatag aacttcttga aaaaatcggt gtctcgaagg
    10501 aattatttcc accattgaca gagccgggaa cgatactagg agatctgcaa aaagaacaat
    10561 tttcaaaata tgatttgcca gatgtgacct ttatcacagt agcaagtcat gatacggcgt
    10621 cagctgtatt aggaacgcca ggtcaaggag aacgttgggg ttatatcagc agtgggacat
    10681 ggtcgctttt agggattgaa acaaagatcg ccaatgtttc tcgcaatgct ttttctgaaa
    10741 attatacgaa tgaatggggt gcccataaca cggtgcgttt tttgaaaaac atcatgggga
    10801 tgtggttgat ccaggaagtt gcccgaatgc aagagtacga gtacagttat gcggaattgg
    10861 cagagcttgc tgcaaaagaa aaaccgctgc agcaagtgat ggaaatcaat gaccctcgat
    10921 ttttgaatcc cccaaatatg atagaagaga ttcagcaata ctgttttgaa acagagcagc
    10981 cagtccctgt ctcatcggga gctatcgctc gttgtgtgta tgataatctt gctctttgtt
    11041 acgcacaaga gctgatcaaa gtagaacact taactggaac gaaattggaa aagctgcaca
    11101 tcgtaggggg cggatcgaat aatcgtctac tgaatcaatt gacagcagat tatgccggaa
    11161 tcattgtaga agcaggacct agtgaagcca cagcaatcgg gaatgttgcc atgcaaatgc
    11221 tcagtacagg tcagttttcg aatatagaac aagcaagaaa tgtaatcaaa cagtcgtttc
    11281 catgtgaaac tttttaccca gatgaaaata agcaaaacac gatggaatgg tacgtgaata
    11341 aaatcaaagg agtaatcgta tgaacacgat tgaaagaaat tacgaacaag caaaagaaaa
    11401 atatgcaact atcggtgtag atacagatgc tgtactagaa aagatacagg acatcaaaat
    11461 ttctatgcat tgctggcaag gagatgatgt caaaggtttc ttgacaccgg atggcgaatt
    11521 gaccggtgga atcatggcga cagggaattt tccaggagcc gcacgaacac ctgaagagct
    11581 tagacaagat ctggagaagg cttattcgtt gataccgggc aaacacaaat tgaaccttca
    11641 tgctatctat ttggatacag aagaatctgt cgatctaaat gaaattgagc cgaaacattt
    11701 tgaaaaatgg gtagagtggg caaaaaaaga agaaatcggt cttgacttca atccgacttt
    11761 cttttcgcat ccaatgatga aagacggctt tacattggct catccagata aagaagttcg
    11821 tgaatactgg atcgaacacg ggaaaagaag ccgcaaaatc gcggagtact ttgggaaaga
    11881 aactgggcaa acaagtatca ataacttttg ggtacctgat gggatgaaag ataatccgat
    11941 tgatcgtttt accccacggg aacgtttaat ggaatcactc gatgaaattt ttacagaaga
    12001 gttagatgaa cggtatgtac aagatgcagt agaaagcaaa ctatttggtt taggcgctga
    12061 agcttataca gtcggttccc atgaatttta tatgggttac gggttgactc gtgacaaact
    12121 tgtttgtcta gatgctggac attttcatcc aacagaagtg atttccaata aattatcttc
    12181 attagcttta ttcagcaaag gaatcatgtt gcatgtttcc cgtccagtgc gttgggattc
    12241 agaccatgtg gttttgatgg atgatgaatt gcaggaaatc gctaaagaat tagtccgtaa
    12301 cgaattgttg gaaaaaacaa atatcggtct ggatttcttt gatgcaacga tcaaccgtat
    12361 cgctgcttgg gtcatcggta cgagaaatac acaaaaagca ctgctgaaag ctatgctgga
    12421 accagttgag cgattgaaag aaatggagct ggcttttgac tttacttcaa gaatggctta
    12481 tacggaagaa ttgaaagatt tcccgtatgc cgatgtatgg aattatttct gttacaaaaa
    12541 tcaagttcca gttggtttgg attggctgga agaagtccag gaatatcaaa aagacgtgtt
    12601 ggaattaaga aaataggagt gtggctatat tgaaaaaaat agatatttta caagcgcctt
    12661 atgtgaaaga aatgatcgaa acgacgtcga atctttatcg cttgggatgg gatgaaagaa
    12721 atggcgggaa tatttcttat ttactaaaag aagaagagat cacaccgttc ttggatcctg
    12781 aaaaagtgat ccgccgcatc tcgatgattt ttgatgccag ccgtttagca ggatgctatt
    12841 ttattgtaac tggttcagga agatatttta aaaatgtagc gagcgaacct gccgagaatc
    12901 ttggaatcgt tcgagttgct gaagatggca aaacactgga acttttgtgg gggctggaaa
    12961 acgaatgcct tcctacaagc gagttgccaa gtcatttcat gagtcatatc gcccgtttag
    13021 aagtagaccc agataatcgg atcgttatgc attgccatgc tagtcatcta ttagcgatga
    13081 gttttacgca tgaattagac gaacgttcct ttagtcgtac gctatggcaa atgtgtacgg
    13141 aatgcttagt tgtttttcca gaaggaattt ccatcattcc gtggatggtt ccgggaacaa
    13201 atgagattgg ggaagcaaca gctgaaaaaa tgaaagaaac tcgattggtt ttatggccac
    13261 aacatggaat ctacgggtca ggaaaagaca tggatgaagt ctttggtttg atcgagacag
    13321 cagaaaaggc agcagaagtc tatacgtacg tcaaagcgca aggaccaatc ttgcaaacaa
    13381 tcacggacga aaacctgtgg catttagcag atgcgtttgg tgtcactcca aaagcaggtt
    13441 atttggagga agttcatacg aaagcaggtg tctgacatgg tgaaaaaagc tttttgcatg
    13501 aaagtttacc cagaaaaaca agaagaatac aaaaaaagac atgatgaact ttggccagaa
    13561 atgcgtacag cacttcgtga acacggggtc atcgactatt cgatctatct tgatccgagt
    13621 acaagtttgt tatttggtta cttagtgatc gaagatgaaa agttatggga aaaaatggca
    13681 aatacatcta tcaatcaaaa atggtggaag tacatggaag atatcatgga gacaaatcca
    13741 gatgcctctc cagtcagttg ggaattgatg ccagtctttg aactgtagca acaagcaact
    13801 gggcaaccgg ttgcttgggg ttctttcgtt tttattgtga aaaataatac aaattaggag
    13861 gaatagaaaa tgagtaatcg aatgatttta aatgaaacat cgtattttgg aaaaggagcg
    13921 attgagtcga tcgtccctga atttcagcag cgaggattcg gcaaagcagc tgtcattact
    13981 gacaaaggat tgatcgagca cggtatagca acaaaagtaa cagaacttct agataaagca
    14041 gcaatcgctt atgctttata cgacgggatc gtacctaatc caacgatcca aaatgtaaaa
    14101 gatggagtgg cttttgttaa agaggcagag gcagactgtt tgatcgcaat cggtggaggt
    14161 tcgccaatcg atacggcaaa agccattggg attattttga cgaatccaga gttttcagac
    14221 gtcattagtt tagaaggtgt tgctgatacg aaaaatccat gtttacctat attagctata
    14281 ccaacgactt ctggaacagc agcagaagta acgatcaact acgtcatcac agatgaagta
    14341 aatcaccgaa aatttgtttg tgttgatcct catgatatcc caatcgtcgc gttcatcgac
    14401 agcgatatga tgatgggaat gccgaaaaaa ttggctgcat caacaggtat ggatgctatg
    14461 acgcatgcta ttgaagggtt tatcacaaaa ggcgcttggg aaatgactga tatgctgcat
    14521 ttaaaagcaa tcgaaatcat cggccattct ttggaagctt ctgtagatgg cgatcaaaat
    14581 gggcgagaaa aaatggcatt aggacagtat attgcgggaa tgggcttctc aaatgtcggt
    14641 ttaggactgg tacacgggat ggctcacccg ttaagtgcat ggtacaacat tcctcatggc
    14701 gtagcgtgtg cagcactttt gccaactgtc atgaaatata acaaagaata tactggagaa
    14761 aagtaccgtg agatcgctct tgttctaggg attaaaggag cagctgaaat gtctttagaa
    14821 gatgtaagag aagcggcttg tggtgagatc gatcggttga gcaaagcggt tggaatccca
    14881 gaaaccattt ctgaattagg tgtaaaagaa gcagatattc cagcaattgc agaagatgct
    14941 ttgcgagatg tatgcacacc aggaaatcca agagaaacga cagtagaaga aatcattgct
    15001 ttatatcaaa gtttgatgta aaaacgaagc ggatcttcaa cattaaattg cctgtaaaga
    15061 gaaatggtag tttatatctg gatatctctt ttaaattgat ggtatttccg ctataatttt
    15121 tcttataaag aaataggaga atattatatg gaaaataaaa agccttatgg aactgtattg
    15181 atcaaagcct ctaaaatact agactattta gcggaaaacc aagatgtctc tttgcagacg
    15241 atcgcaaagg gtgtgggaat gacctcttct acagtcttga aaatactaga tactttgttg
    15301 atgatcggct acgtaaataa aaattcagag aaaaactatc gtttaggtgc aaagcttatt
    15361 cgctacgcga ataaaaacat tgaacaaatc gatttggtag agttgacttt accgttttta
    15421 gaacagctgc agcaaaagat cgatgagact atccacttag gggtattgga caacaatgag
    15481 atcttgtacg tgaacaaact agatccaaaa catcaaacga tccgcatgtc ttcaaaaatc
    15541 ggaatcaccc gaccattata caattctgcg atgggcaaag ccgtattagc agaattcagt
    15601 gaagagcagt acactgatta tcttgaaaag catccgttga tcccttacac agaatatacg
    15661 atcaccaatg cattacggtt gaaaaaggaa atccaaacgg taaaagaaac ccacatcgca
    15721 tttgacgatg aagaaatcga acgtgatatt ttttgtatag gagcctcaat cgtgaaaaac
    15781 gaacaaatca ttggtgcatt tagtgtaagt atgccgaaat atcgtttaac agatgagaca
    15841 aaagaacaaa taatcagtgc cctcaagcaa acaaaaagcg aaatcgagca aagaattcaa
    15901 aaataatgaa aaaagcgttt gcaatttttc ttttctgccg tataatatgg gtatagattt
    15961 ccaagttata aaataaaatt tctaaatagg aaataggtgg agaagatgaa aaaaattgat
    16021 attttatctc gattgaaaaa cgccggagtg attgcggtgg tacgcggaaa aagcaaagaa
    16081 gaagctttga atgcttgcca cgcaatcatc aaaggtggtc taactggtat tgagttgact
    16141 tttacagtcc ctcaagcaga tcaagtaatc aaagaacttt tgtcattcta caaagaccaa
    16201 ccagaaatag tgattggcgc aggcacagta ttagacgctg tcacagctcg cttggctatc
    16261 ttagctggcg cagagtatat tgttagccct tcctttgatc aagaaacggc agagatgtgc
    16321 aatctgtatc aaattcctta cttaccagga tgtatgacta ttaccgagat caaaacagcg
    16381 ctgaaaagcg gggtagatat cgtcaaacta tttcctggaa gcgcttatgg accaagtatc
    16441 atttctgcct tcaaggcgcc gatgcctcaa gtcaatatca tgccaactgg cggtgtgagt
    16501 ctggacaata tgaaagagtg gttcgatgct ggtgtcgtaa cagtcggagt aggtgggaac
    16561 cttttagcac ccgcagctac aggagatttt gacaaagtaa cggaagtcgc gcaacagtat
    16621 gcagcaaaaa tgaaagaaat caagaggtag ctgcttatgg gaaaagtagt gacactcgga
    16681 gaaatcatgt tgcgattgtc cacagagcag ggaacccgta tcgattcaag tgaacgattc
    16741 gctgcccatt atggcggagg ggaagcaaac gtagcaattt ctttagctaa ttacggacat
    16801 gaagcgattt ttgctacaaa agtacctgat aacgcattgg gaaaagcagt caaaaagcac
    16861 ttgcaatgtt atggagtaga tacgaaacag gtattatctg gtggatcaag actaggtact
    16921 tattatatgg aaactggtag tggcgaacgc gccgcctcag tgatctatga tcgtgccgga
    16981 tcaagttttg ctgaaatgga tcatttggaa tggtcgctag aggagttatt ccaaggaata
    17041 gatatatttc atgtttcagg aatcacccca gctttgtcta aaaaatggca aaaaatgacg
    17101 aaacaggtga tggaagctgc aaaaaaagct ggctgcctga tcagcttcga tatcaactat
    17161 cgtggaaaat tatggacaca gcaagaagcc agcgaagtaa tccatcagct tttaccttta
    17221 gtagatatct gctcagctgg aaaaatggat gctctttatt tgttaggtat cgagaaagcg
    17281 ccagaagcag aagaacaacc tctaatttat tattatcaaa aaatgcagga aaagttcccg
    17341 aatattcagg tgttttattc gaccaagcgt accgttcatt cagcaagtga aaatgaattg
    17401 aaggggacat tgtggatgaa ccatgaatat gtggaatccc aagtccatag aatcacccca
    17461 atcgtggatc gagtaggtgg cggagatgct tttgcaggtg gcgtgctcca tgggatttta
    17521 tccaatatgt ctccgcaaga aatcattgat tttgctacgg cagcttctgc cttgaagcat
    17581 acgatccatg gggactgcaa ccaatttagt caagaagaag tcgcacaatt tttagcatgc
    17641 ggctctggaa aaatcattcg atagaaggag tagaagaaaa tgcaagaaat ggaaacacgt
    17701 tatacacaca gtccagaaga tatccgtcat tattcaacgg aacaattgag aaaagaattt
    17761 ttagtagaaa aggtatttga accagggaaa atcagtttga cttatacgca caacgatcga
    17821 atgatctttg gtggagtgac acctactgaa aaagaattag aaatcacatt agacaaagaa
    17881 ctaggtgttg attacttctt agagcgtcgt gaactaggtg tgatcaatat cggtggacca
    17941 ggattcatcg agatcgatgg gcaaaaagaa gaaatgaaaa aacaagacgg atactatatc
    18001 ggaaaagaaa cacgacatgt tgtattttct tctcaagatg ctgaaaaccc agctaaattc
    18061 tatattagtt ctgttccagc tcatcataaa tatccaaatg taaaaatcag tatcgaccaa
    18121 gtaaaaccaa tggagactgg tgaaggcctg acactgaatg aacgtaaaat ctaccaatac
    18181 atccatccaa atgtatgcga aagctgtcaa ttacaaatgg gttatacgat tttagaacca
    18241 ggaagcgctt ggaatacgat gccttgtcat acacatgaac gtcgtatgga agcttatgtc
    18301 tactttgatt atgccaatga agatacacgt gtgttccaca tgatgggcaa accagatgaa
    18361 acaaaacatt tagttgtaga caatgaacaa gccattattt cgccaagctg gtcgatccat
    18421 tcaggtgtag gtacaagcaa ttactcattc atctgggcaa tgtgcggaga aaatatcacc
    18481 tatacagaca tggatatggt gcaaatggat caattaaaat aagcataagg gagagaaaat
    18541 gatggaattt agtatggata tgtttcgttt agatggaaaa gtagcattag tcaccggtgc
    18601 tgtctatggt attggatttg agattgcacg ctctttagct acagcaggag caaaaatcgt
    18661 atttaataac ttgaatcaag aatcagttga tgaaggtata gcgcattata aagaagcagg
    18721 catcgatgca aaaggttatg tctgcgatgt gaccgatgaa gaagcggttc aagcgatggt
    18781 tcaacagatc aaagaagatg taggttcagt tgatattcta gtcaacaatg cagggatcat
    18841 caaacggaca ccgatgatcg aaatggatgc ggcagacttc cgccaagtaa tcgacgtgga
    18901 tttaaatgcc ccattcatcg tttcaaaagc agttattccg gatatgatcg aaaaaggcgg
    18961 cggtaaaatc atcaatatct gctccatgat gagtgaatta ggacgcgaaa cggtcagtgc
    19021 ttatgctgcg gcaaaaggcg gattaaaaat gctgacgaaa aacatcgctt ctgaatacgg
    19081 tcaatacaat atccaatgta atggaattgg accaggttat attgctacac cgcaaactgc
    19141 tccattacgc gaaacacaag aaaacggtga acgtcatcca ttcgatcaat ttatcgttgg
    19201 acgtacgcca gcagcacgct ggggcgagcc aatcgatttg gctggaccat ctgtcttctt
    19261 ggcttctagc gcatctgatt tcgttaacgg ccatgtcttg tatgtggacg gaggcatact
    19321 tgcctatatc ggaaaacaac cttaaaagag ctttagtaca ctgtttagtg gagaggttgt
    19381 gaaatcagcg gattgaacga ataccgaagg acagctgatt aaacatcatt tattcgaaaa
    19441 aagaggctgg gacagaagca tttaactcca agaaataaga aggaattcac gaaaattgct
    19501 tttcaaattt ttgtgaattt cagcttattt ccgaaggagt tgcttttact tccgccgttt
    19561 gtacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tcttttctgt
    19621 gcgtcttcca tacttcacta agtcgatccc tcttgcttcg caccaatcct tcactggtcc
    19681 ggaaaagatc agagattgtt ggtgtagggc cgcggtattc gttgcgccaa ccatagtata
    19741 aagcagacga agctcttctt gccattgctt catcagtata atagtttctt ctacgccgtg
    19801 attcatcaga tgcgtcagta cggttcctga agtacccacg gcatttgcgc cgagacaaag
    19861 ggctttaaat atgtcatagg cattgcggat acctccggag gctaggattt ccatagaagt
    19921 gtctgcttca tttgcttcaa gcaatgaaga gactgtcgat tgcccccagt cagctaaata
    19981 actcagttcc cgcttggagc gacgggcgtt ttcgatctgg gtgaagcttg ttccgcttcg
    20041 accgctgata tcaactgtat gaactcctaa agcagctaga tcattgagcg tttctcttgt
    20101 catgccaaaa ccgacttctt ttacgatgag ggggacgtca atggctgttt gagtttcttg
    20161 aataagtact ttccaattgg taaaatcacg gtctccctca ggcataacca actcttgagg
    20221 tgcattcaaa tgaatctgta aggcatctgc gtgaaacaat cggatagctt ctttggctct
    20281 ttcgacagaa gtccctgcac cgatgttggc aatgattttc ccgtgaggat attcttgtcg
    20341 cataatcgta taagtatcgg ctaaagaagg gactttcaag gcagcgctga ctgaaccagt
    20401 ggcaatcatc aaatctgctt ctcttgcgac gattgcaaga tcttggttga ttttttttgt
    20461 tttttcgctt ccgccagtca tcgcattgat ataaaatggg ctgcttagtg taaacccaac
    20521 tgcctgtgta cttagatcga catcagaaac tgctatttgc ggcaaagggt tatggatgat
    20581 ccgtacgaag tcaaattcgt ttttttgttt atcatgaaag gctttagcta gtgaaacatg
    20641 ttcgtctttt cgattcatcg gttttccttt ctttgatcgc tatagacatg taacggcagt
    20701 ggagtgattt ctgctttttc ccatgcactc attaaaggaa gaatgccaga tttctggtca
    20761 acaattacga ttccgcaatc gcccccacct gcaccagaag attttgcggc tccttcatac
    20821 tgttcagcta aattacacaa tttgttcaaa gcaggcgttt cgatgacgac cccagtgatt
    20881 gcagataaat catgcagtaa ttgtcggttt tttcgaatca tctgttggat caacgttaca
    20941 ttattttctt taaacccttt gatcatttcg ttgacacatt ctgtactatt ttttaagaaa
    21001 agctgataag ccaccatttt atcttctctc gaacggtgaa cttgatcgac caaatcagaa
    21061 gtagaggcag ggctacccgt ccaaccaatc aataaacgta aatcttcagg agcaatcaat
    21121 ggctcaatgg atagacctgg ccaatccagg gcaagtaact cactgataga atgctgagtt
    21181 tcttgttctt ggagccaagg atggtcgaag gttgagaaag cgatccagcc accataacag
    21241 ctagcagcga tgtcgccgca ggaaccatta tcttgaactg ctaaattggc tagtgctgca
    21301 atcttgaaaa tctccaactg agataaattc aacgcgtaaa aaacattcaa ggctttgacc
    21361 gttgcaacgg tcacggcacc gctagaaccc aatccatatt tccggccatt tgaactgtct
    21421 aactcacttg ttactttcag atcataaaag gataaaagaa tgtttttttc ttgtgcatac
    21481 ttttcagtca agcgaatagc agcaaggata taatgaaaag gattttcccg aatatctaaa
    21541 actaattctc cgttgcgtct tgtccaacgt acaggcatcc cactatattg tgcagattgg
    21601 atacttccga cttttcgtgc agattctaca gtgactgtca cgaattgatc gactgcagcg
    21661 ataactgctg gatggcctgt ttcaacaact gcatattctc cggcaatata gagtttgcct
    21721 ggtgcagata cttcaatcat aaaaatctcc ctttattcaa taatcgcaat tcctggaccg
    21781 gcatgggcag taattacttg ttctttgcta aataatttgg taaatgtttc ttgtacctca
    21841 gataaatggc tattttccac taaaactttg acattagggc cagcatccat cgtaaagtag
    21901 catggaatgc cttgctttct caattgtcgc accgcatcca tcgcttttaa gctgtcagga
    21961 gaccagtaag tgaatggagg ctgggcagct aaagtcgttc catgcatttt tagtccgttt
    22021 ctttccatcg tttctccgag aagttggaaa tcttttgttt tgattgcttg tttcaattga
    22081 tatagatccc cttcaacgga atctaaccag ccttgataaa aactggatgt ttcgactgtc
    22141 cgacgcatcc catttctgct ggacacttct tttttctggt cgttgatcaa aacgaaaacc
    22201 attgctaaat cgtcttcgaa agaatcggaa gggactggct tagcgtaaga acttaagtcg
    22261 tcatggcctt tttcccattc gacgaaacca ccgaaaatac ttcggcaagc tgaaccagac
    22321 ccgcgtcgag caaatcttga aagagagaga tcatctaatc caagcttaag agcagtattg
    22381 caagcgcctg ctaaagcagc tagcccgctg gcagaggagg ctaatccagc tgcggtagga
    22441 acgaaattct gactgattac tgaagctttt agtgagaggc cggcttcttt acgaaaaagg
    22501 tcaagaaact gactgacttt ttttgttgct ttttcgtctt gcaaggtgcc atctagataa
    22561 aattcatcta ccatatagct atcagaaaag atgacttctg tctctgtata aaatgcatcc
    22621 aatgttaacg aaaggctgtt gttcattgga aggatgagtt cttcattttt cttaccccaa
    22681 tatttgatta gagcaatgtt ggtatatgcg cgtgctttgc ctttaaacat aggtatgtac
    22741 tcctaatcct tgtatccaag tttcagcagc acccgcatct tcaagtgctt gactgatttc
    22801 ttgggctttt gtttttgttt gtgctaatgc aatcatacag ccaccgcgtc caccgccagt
    22861 taatttggca cccaatgccc cggtatcttt tgctgtttgg atcaatagat taagaaaatc
    22921 attgctgatt gtcagctttt ccagaagtga ttgtgattca tccattgtct gtgccaacgt
    22981 ttctggtgaa ttttcaatga ttgcttgttt tgcttgcttc gtcaagtatc ctaatttttg
    23041 gatcatttgt ccagtttcat gaggctgggt ttcaaagagg tgagcgacat ctttgacggc
    23101 ttctcttgtt tgtcctttga ttcctgtatc agcgacaatc aaaaaagcat cgatgttcaa
    23161 agaaaagtag tcgaaaggat gcccttttgt aaaataaatc ggctgcaagc tgctagtagc
    23221 ggctgcatcg attccactag gattaccgtg ggcgattttt tccgaaagct ggacattttc
    23281 taatagtatt tcacgagaca aaggaaatgc taagtaatca taaaaagcac gagtgactgc
    23341 tgtggcgact gcagcgcttg atcccattcc acgttcagca ggaatcgtgc tttcaatggt
    23401 cagttgcaag tttttttgga tgtcatgctg tttttttaag tgaatgaata attttttgat
    23461 attttttaat gcatgaggag cttcggctaa atttcctgaa tagtaggaag aaaccagttg
    23521 atcgtccatt gcatccagct cttcaaggaa tgcggtgact tttgttgcat agaaaggaaa
    23581 ggcaatcgct ggttctccat aaacaaccgc gtgctcgccc atcaatatga tctttccgct
    23641 tgattctcct tggccatagt ttgccataat ctaactcctt ctcacgtaaa agtgattttt
    23701 aagcaaaaaa taaagcgccc aaaacgagtt gcgctgcttc acgaattttt ttgtccacta
    23761 agggtctcaa aaaacattcc tcctttattt tactaaaacc atctcagaag taaaactaag
    23821 aatacgtttt ctaaagaaaa aacacttttt tcttaagaat tttttgactt tttttcttct
    23881 ttgactagaa aaaaatacaa ttgacactac tagatattgg gggtatattt aaaaaggttg
    23941 gagttaaaca caatatttag tgatgaagtg agctggaggt aaatgagcgt gatcgtttta
    24001 gcaggtacaa ttggtgccgg aaaatcaagt ttaacagaaa tgatggctga acactttgat
    24061 agtcaggcat tttatgaatc gattgatgat aatgaagtct tgcccttatt ttatgcaaac
    24121 cctgaacagt atgctttttt gctgcaaatt tattttttga ataaacgttt tgcaagcatc
    24181 aaacaagcaa tgaaagatga caataatgta ttggaccgtt cgatttatga agattcactg
    24241 cttttccatc taaatgcaga tctaggaaga gcaacagaaa cagaagttcg tgtgtatgat
    24301 gaactgctgg aaaacatgat ggaagaactt ccctatgctg cccataaaaa acatccggat
    24361 ttattagttc atattcgtgt atcttttgat acgatgttgg agcgtatcga aaaacgtggt
    24421 cgttcgtatg aacaattgtc ctttgatcca agtctatacg attattacaa agaattgaac
    24481 agacgttacg atcaatggta tgaagaatac aaagaaagtc caaaaatcca aatcgatgga
    24541 gaccgctata attttgtgga agatcctcaa gcaaaagaag aagtcttgaa aatgatcgaa
    24601 gaaaaattag cagagattcg ccaaaccaaa gtagccattt gatttacagt tgctcaaata
    24661 aagggtaaac ctttatttga gcgacttttt tctttatact ggaaacaaat gtcaggagga
    24721 tagagtatgc gggaaaaaca ctattttaat caagaccgta atttttggat caatgtacag
    24781 caaaacgatg tgctagctgt taaaaactta aaagaagaat atggcattag tgacgagatg
    24841 ctgacttatt ctctagataa aaatgaacgg gcgcgcgtag agtacgattc ttttgatgaa
    24901 gcgttactgc ttgtatcaaa tgttccgcac cagcaaaaag tcgataatca ttatgaaact
    24961 agtcctatcg cttttatttt aaaagaagac ggtttgttta catttaccac gccgaatacc
    25021 gagtacgtga ttcgactaat tcgatcgctt ttggaacgaa tgccagacat gagtgtatac
    25081 agtttattgt ttcgtacatt gtttttgatt tctgattcct tttttccttt aatcgaggaa
    25141 gtgaatagtg aacgtcagcg gcttaatctg aaattaagag aaaaaacaac gaacaagaat
    25201 ctgctgcaac tttcagattt agagatcggt ttggtttact tagttacagg aacaaaacaa
    25261 aatgtagtac ttttagaaca aatcaaggca ttggctattt atcgcaagtt gtcagaaaag
    25321 gaaaaagaag agttggacga tgcgctgatc gaagcacgac aagcagttga aatgacgaac
    25381 ttagcttcgc agattttaga ccagctctct ggcacttata ataatttgct gaataacaac
    25441 ttgaatgaca cgatgaaatt tttgacggtc tggtcgctga tcttgaccgt tccaactatt
    25501 gtgacaggat tcttcggaat gaatctccag ttacccttta cccattccgt tttcggatgg
    25561 ggaatcgcct tgatcatcag tttggtctta tctatttgga tgcttattgc actgtggcgg
    25621 cggattcgtt gatctagtct ttgatctgca aaccaagggc ttgggcgatc acaagtgctt
    25681 gctcttggtt gacgattagt cccgccaaag aatcttgaga taaagcgatt gctgtgaaga
    25741 aattagtcgt aaagtctaat cccttcagag aagttcgaaa ccagttcgaa ccggtcaact
    25801 gattgtcaga caaaaaaagg tttttccaag tgacctcata aaattcgctg tcttctaatt
    25861 gacaatgatc gaaggagaca gctttcaagt ttgtactact aaaggaagcc atcgaagcaa
    25921 tacaatcagt gaaagtacag tctcgaagat aactttcggc aaaattcgta cctgttaatt
    25981 tacattgatg aaacacgact tggtggaaac tgccaccaat ccactccaga ttggaaaaat
    26041 cacatttttc aaaaatgaca ttactgcatt caaaatgacg taaaactgtt ttttgcatcg
    26101 taagtttttg gatatgactc tctcttaata ccatattttt ggcatcgaga tagctttggt
    26161 cacccccgtt gatcagaaga ccagagaaga tctcctcatc ttctaaataa aactctccct
    26221 ttgtaagaag aggaagagaa ggtggaatag gttcgatttt tttcatgtgg gttcacctcg
    26281 tttttcttta gtatagcaaa gaagcgggaa tcactgctag aaagtctgct aatctaatca
    26341 ttttcagaaa aggaagacaa actgtatgaa aaacattgac aaaaagatta gtttttgcta
    26401 tactatgatg gttgagaaat aaaagcagaa gcacccgctt ctcgccttag ttgataatgt
    26461 cactgggcta gatagttttc actggttcta ttaggtgaag tacgcgcggg ttctgtaaga
    26521 gaactcgttt ttttattgcc gtttattcgg taagcttttc tttctctcat aatatttgga
    26581 ggtgaatgac catagcaaag gatatgatgg ttaacgacgg cattcgtgca cgagagttac
    26641 gattgatcgg ttcagatggt gagcaattag gagttaagac aaaagcagaa gcattgaata
    26701 ttgcagaaca agcaaacttg gatcttgtgt tagttgcccc tggtgcgaaa ccaccagttg
    26761 cgcgaatcat ggactacggt aaataccgtt tcgaacaaca aaagaaagac cgcgaagcgc
    26821 gtaaaaaaca aaaagtgatc aatgtaaaag aagttcgctt aagtcctacg atcgacttga
    26881 atgacttcaa tacaaaactt cgtaatgcac gtaagttctt ggaaaagggc gataaagtga
    26941 aagcttctat ccgtttcaaa ggccgtgcca ttacccataa agaaattggt caaaaagtct
    27001 tgaatcgctt agctgaagaa actgcggata ttgcaacagt ggaacaaaaa gcgaaaatgg
    27061 acggacgcag catgttcttg acgctggcac cgaaaaacga cagtaagtaa tttgaatgac
    27121 taactggctg ttggacagcc gatagtcgga taacgaaaga ttttgaggag gaaatatagt
    27181 catgccaaaa caaaaaacac accgcggatc agcaaaacgt ttcaaacgta ctggtaaagg
    27241 cggattgaaa cgcttccgcg cgtttacaag tcaccgtttc cacggcaaaa caaaaaaaca
    27301 acgccgtcaa ttgcgtaaag caagcatggt ttctagtggc gacttcaaac gtattcgcca
    27361 acaactagca aaaatgaaat aagaagtaca aataacagaa tctgaagatt tatttttatt
    27421 aggaggaatt atacatggca cgtgttaaag gtggaacagt aactcgtaaa cgtcgtaaaa
    27481 gagtgctaaa attagcaaaa ggttactacg gttcaaaaca cactttattt aaaacagcaa
    27541 aagaacaagt aatgaattca tacaactacg catatcgcga tcgtcgtcaa aagaaacgcg
    27601 acttccgtaa attgtggatt gctcgtatca acgcagcagc tcgtatgaat ggcttgagct
    27661 actcaaaatt aatgcacggt ttgaaagttg ctgaaatcga catcaaccgt aaaatgttag
    27721 ctgacttggc tgttaacgat gcagcagcat ttactgcatt ggctgaccaa gcaaaagaag
    27781 ctttggctaa ataagctaaa caattataaa gaggaaagaa gataacgaaa agttatgttc
    27841 tttcctcttt ttttatttac aatcctccat caaatgaaac aggacaataa cgtataagtc
    27901 taaaatagca taattaaggt tttttaattg aaaactgaac atttatggtc atcctttatt
    27961 gaaagcgcta actacataag ttagaatgag tttgactgat atattagttt gcttatcaga
    28021 aaaacaaaat ggacggagga ataacatgag tgatattatt attggtattt tgttagttat
    28081 ctcattcttt ttcatggtct ggtactgtgt aaaaggctac aacttgatgg tcggctttgc
    28141 catcatggct actgtatgga tgggattagc gctagtcgga aacacgtttt caccaaaccc
    28201 ggcgatggaa gggcaaggag taattgatgt gttgacgcat atctatacga cggggccagc
    28261 tgagtacgcc aaatcgatct tggtaaatgt cttctttggt gcctttttcg ggcgtgttct
    28321 agtagatagc ggaatcgcag ctaccctgat tcgaaaggta gttgaacttg gaggggacaa
    28381 gccaagaatc acgatgagct tactatgtat tgtaaccgct gttatattca tgtcgatgac
    28441 aggaatcgga ccagtgattt ctattgcagt tattgtgttg ccaatcttga tgtcattagg
    28501 tatttcagta ccggctgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
    28561 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
    28621 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
    28681 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
    28741 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
    28801 agttttaggg gtcgttcttt tagatcttcc aatcatctta ggttttattc tagcaggtat
    28861 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
    28921 tgcgaaatta tttacagatg gcgcagtaga tgttgcacca atggttggtt tcttgatgac
    28981 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
    29041 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
    29101 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
    29161 attagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caatcacaac
    29221 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
    29281 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggagc
    29341 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
    29401 aaaacgatta gatgaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
    29461 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
    29521 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagccgcagg tgtcgttcag
    29581 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
    29641 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
    29701 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
    29761 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
    29821 cacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
    29881 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
    29941 gaagcagcat cggtaaaatc gatccctaca acgatggctt ctgacatcac aaaattatat
    30001 ggattgacaa gaagaactcg gacagcagca atcaatgcat ctattttacc aaaaatgcta
    30061 gatacggcaa cctctacaga agattccgta cgagaagcag gggtcaatgt ttctttgatg
    30121 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
    30181 accatgcttt ccggtccagc agcatcagtc atgggctctt tgatgtactt gagagcgtct
    30241 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
    30301 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
    30361 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
    30421 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
    30481 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
    30541 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
    30601 aatgtccttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
    30661 aaagccatcc aagcattagc tgattattgt catacgacgg ttgaagacat cgcagaacag
    30721 atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat
    30781 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
    30841 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
    30901 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
    30961 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
    31021 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacagac ttcaaaagta
    31081 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
    31141 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
    31201 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
    31261 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
    31321 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
    31381 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
    31441 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
    31501 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
    31561 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
    31621 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
    31681 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
    31741 cgatgtagga gaaatttgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
    31801 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggattc gattgatgac
    31861 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
    31921 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
    31981 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
    32041 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
    32101 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
    32161 gatcttagaa gaaataaaaa aaatagaaaa aagatgtacg gagtatgatt tagattacaa
    32221 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
    32281 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
    32341 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaacggtgtt
    32401 caaaagcttg catctgcggt aataagtcca gccaagttaa aaacataaga agctattttt
    32461 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
    32521 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
    32581 ttgccgtttc tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
    32641 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
    32701 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
    32761 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
    32821 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
    32881 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
    32941 aaaaaaagct tttttatttt gagcgattac tgcctgatcg gtttgttcta gtattttttc
    33001 tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga tcggtttttc
    33061 caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata
    33121 agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc
    33181 tcgaatcggt gtacgcccca tagctagttg ttcttctaac actttatcgg agattttact
    33241 tccaggtgct aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg
    33301 caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa
    33361 atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat
    33421 gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg
    33481 tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat
    33541 gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg
    33601 cattacttta acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac
    33661 tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca tcatccaaag
    33721 aagcgtacgc ttggaataac tgatctaact ccggattgac tttgctcagc ttcactaatc
    33781 gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt
    33841 cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt ccccgtatac ttttggatag
    33901 ttggatggat gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt
    33961 ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta
    34021 ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc
    34081 ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga
    34141 ctcatgcctc gttttccatt gattttttac aaaaataatt catccatttc ggaagtgctt
    34201 cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac
    34261 tatgaagata aatagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc
    34321 ctgtgatcat cccagctaaa gtgtcaccca tgccgcccgt tgccatagca gcagaaccta
    34381 aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga
    34441 gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg
    34501 gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat
    34561 agggtagcaa aagatgatta gcagcaagta aacttaaagc agagccatca atcaccagcc
    34621 attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag
    34681 tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt
    34741 cagtatccgt ccaaactaca atcattgctt ctggcagatg actgtgaaga gcactccgat
    34801 ttttttcatc agttactaca gtcgtgagac ccgctccgct gtgtacggca gctaaagcac
    34861 tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg
    34921 ttcctttatg tgaaattttt ggacgaggtg tgatgactct tttcactagt tgttcgttta
    34981 tcagcatcta aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag
    35041 ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg
    35101 aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag
    35161 tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg
    35221 agcgcgatga ctcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat
    35281 tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact
    35341 atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg
    35401 atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg
    35461 gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg
    35521 cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg
    35581 gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac
    35641 cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg
    35701 tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg
    35761 tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa
    35821 gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag
    35881 taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg
    35941 cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag
    36001 ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc
    36061 atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat
    36121 tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca
    36181 tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg
    36241 aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac
    36301 attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca
    36361 ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac
    36421 gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg
    36481 aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc
    36541 gtttggcgaa ataattaatt aaaaagagga tcaagaattg caaattattt ttgcagtctt
    36601 gatccttttt ttactttcta ctgtggtatt catgataaac atgatataga ttcaattcat
    36661 attctttctt agcgttcgtg acgactgtat ccaaaatcaa ccctgttact aatgaaagaa
    36721 gtgctaaagt catcaatcca gtacttaaaa tcgcagaagg gatgcgtgta ataaagcttg
    36781 tcagcatata ttcgcgaatc acaggaatcc ccgtgaccaa tccaaataaa aagaagaaga
    36841 tggtccaaat cccaaagaaa agcaaaggct tataatcttt aaacatttta ataatcatca
    36901 tgatcacttt gaatccgtca gagaatgtat tcaattttga ttcgctgcct tcaggacgat
    36961 cacgataatc gattgggatt tcaacgaact tgaatttttt atctaaagca tggatcgtca
    37021 attccgtttc gatttgaaaa ccagaactca taataggaaa acttttgaca aagatacgat
    37081 tgaatcctcg atagccggtc atgacatcaa gaatttttgt tttatacatt ctagtaattg
    37141 tatttctaac aaggttattt ccaaaatcgt ggaaaggtct cttattctca tcaaagtagg
    37201 ttccatttga taggcgatca ccaatgacca tatctgcttc accagaacgt aatttctcga
    37261 gcaaaccatg tacagcttct gcaggataag tatcatcgcc atctaccatt aaataatagt
    37321 cggcatcgat atcaaagaac atttggcgga tcacatttcc ttttccttga cgaggttctt
    37381 tttttacgat tgctccacct tgaacggcca attcgtatgt tcgatcggtc gaattattat
    37441 catagacata aatatctgct tctggtaatt ctcgtttaaa gtctgcaatg actgttgaaa
    37501 ttgttgcttc ctcattgtaa caaggaatca gaacagcaat tttttcttgt tgttccattt
    37561 ttcactctcc ttaactatag aaaacaaaag ctaaattaaa aatacctcat aatcaaagga
    37621 aatgctagtt tttcccgcta aatatcattt atttaaaaag aaaatactgg aatatatgtt
    37681 tggaaagctc tatctaaaaa accacccact acaaaagaag gacatgatac ctctcttttg
    37741 aatagtgggt gcgtattcgt tctctcactt ttttgacgta ttaatctgtt gcaccttttt
    37801 ttcttgaaat ttctaatacg gttcctgtat gagcatctgc taaaaattca taataaacag
    37861 tttcgccatc ttctattcga gaaagacctc cccgataagc actcgaccga acggcgaatt
    37921 tcctaatagg ttccttttca aaagagatcc aagaaccttc gattgcacct tcttttagaa
    37981 aagatgactt gatattttca agtacttggt ctgctgacaa tacttttttg cgatgatacc
    38041 aaatcgttga agctaccccg ctcaataatc ctagaccgat tccaactgct aacccgcctt
    38101 taaaatagct tagttcgctt tcttcataca tgtggacata cctccttctt ctcacttgtt
    38161 actccttgtg cttattttac cacactctca tattttcttt gaaataaata accgctacca
    38221 gaaaaaataa gctataataa atacgatcgg tctgcagaaa aaatcatatt ttatccgcag
    38281 atcaatataa gtctgagagg agtttagtct gaaatttaga cgaatagcat atggaagaaa
    38341 aaacatttca acgaatcaaa gagttaacag aattacaagg aacaagcggt tttgaacaag
    38401 atgttcgcgc ttatatggaa agtcatatgg aaccgctggt agatgagtta cagttagatg
    38461 gtttgggcgg catttttggt ctgcgccatc atgaagaagc ggatgcaccc cgtgtaatgg
    38521 tcgcagcaca tatggatgaa gtcggattca tgctgacaca aatccaagaa aacgggcttt
    38581 ttaaagtcgt acctctaggc ggctggaatc cttacgttgt ttctgctcag cgttttacac
    38641 ttaaaacctc tactggtaag aattatccat gtatttcttc ttccgttccc ccacatttat
    38701 tgcgaggaac aagcggacaa aaatcagtag aagtaaccga tattttgttc gatgctgggt
    38761 ttgaatcaag agaagaggca atgagttacg gtgtccttcc aggagacaca atcgttcctt
    38821 acgctgaaac gatcaaaaca gccaacggaa agaatattat cagtaaatca tgggacaacc
    38881 gctatggctg tacaatggta ttagaagctt tagaagcgtt gcaaaatgaa acattaggtc
    38941 atacattgat tgcaggcgcg aatgtccagg aagaagtagg cttgcgtggt tctaaagctt
    39001 ctgtcaataa attcaagcca gatctgttct ttgcagtaga ctgctctgca gcagatgata
    39061 cagtgacgaa aaatggcaca ttcggacatt taggagaagg tacattgatg cgtatccaag
    39121 atccaggttt gatcatgctt cctcgtttgc gcgagtactt gctggatatc gctgaaacaa
    39181 acaatatccc ttatcaatac tttgtttcaa aaggcggtac agatgcaggt gctgctcata
    39241 cacaaaacga aggaatccca agtacagtga ttggcgtagt gggacgttat atccatacgc
    39301 atcaaacgat gttcagtatt cgcgattttg aagctgcacg agaaatgttg attcaaacac
    39361 tgaaaggttt ggataaatca acagtgaata cgatcgtata cggaaaataa tgcgatagtg
    39421 aaactataaa atctggcaaa agattgcttt ttgccagatt ttttttataa aaagaggatc
    39481 aatgtttgtt agttcgaaag aaaggtgtat aatttaattg aaaaatacat aaacagaaag
    39541 tagaggacaa agaatgatta tcccaaatag tttagaagaa ttagcaggct acgtagaaac
    39601 tggaaagagt gtatttttct ttactgccgg ctggtgcgga gactgccgtt ttatcaaacc
    39661 gcaaatgcct gaaattgaaa atgacttttc tgattggcaa tttattgaag tagaccgtga
    39721 caagtacata gatgtagccg ctgaatggaa catttttggt atcccaagtt ttgtcgtgat
    39781 ccaagacgga aaagaattag gtcgtctagt gaataaagat cgtaaaacaa aacaggaaat
    39841 cgaatcgttc ctagaaagtg taacaaaagg ctgacatctg ttaggagggg tcaagatgaa
    39901 tcaagaatat aagaatattt tagtaggtat cgacggtagt gaacaagcac ttgctgcttt
    39961 caaaaaagcg gtagaagtgg ctcgccgtaa taatgggaaa gtatatgtag caaacgtaat
    40021 cgatcagcaa ttttataact ttatgggcta tgcacctatt gatcaaaatc tagtcgatca
    40081 gcaaactgaa acagccaagg aaatgatcga agaatgtaaa gaatatggga aatctgttga
    40141 ttacacaaat atagaaggaa ttattgcata tggttctgtt cgtgaatcca tggcagtcaa
    40201 attgccaaaa aaatatgaga tcgatctaat catggttggg cagtcaggtc taaatgcagt
    40261 cgaacgcttt atgacaggaa gtgtagccag ctacgtgata cgtcattcac cttgtgatgt
    40321 cttgatcgtt tctgacgaag aagaacagcc aagtaattaa gcaagtggaa cttgtacgac
    40381 ctttttaatc aattaactaa aacagtaaag ggactgtgac aaaagtcttg tcacagtccc
    40441 ttgttacgaa taaatggtgt tccagaagag gcttcttcgg aaataaagca tgaaaaaaat
    40501 ttcatgcagt gaatgcaatt ttttgttaag ataaatctca gcaaaggata atggaggaaa
    40561 gaacatgatt tttgcttata acaaagcaca cgtcggagac accttgatgg ttattatagc
    40621 cgacgataaa ggaacagaaa acactgttga acgcaaatgg aatgttgctc ggatcaaaga
    40681 tgaacaaggt caagttgttg cttggaactt tttccatatc tccgatcatc taacaatcga
    40741 agggaacggc caagtaagct taacagatga ggaattagcg aaattgaatc gattgatcaa
    40801 agaagcagga ttcgaagaac agcttgaagc tgatcatgaa ccgaagatcg ttgtcgggta
    40861 cgtaaaaaca tgcgtcccac atcctgattc tgaccattta tcgattactg agacagaagt
    40921 agacaatggt acagtattgc aaatcgtttg cggtgcaccc aatatcgaag ctggacaaaa
    40981 agttgtcgtt gcaaaaccag gagctatgat gccagatgga atgatgatct ggccaggaac
    41041 tttgcgaggg gtagatagct atggaatgat ctgttccgct cgtgaattgc atttgccaaa
    41101 tgctccagca aaaaaaggaa tcttagtatt gcctgaggat gccgtagtcg gagaagaatt
    41161 tccaaaataa agaggttgtg acaagtcttt gtcacaacct cttttttatt aattttcact
    41221 attttgattt tgttttaatg ccgattgatc gatcgtcaat tcaacagtag ttgttttttc
    41281 tttcgagcct tcatagtaag tgattttgac agaatctccc acttttttct tatacaaagc
    41341 agattgaagt tctacaccag aagaaacttc tttgtcatcg atcttagtaa tgacgtcgta
    41401 ttgtttcaat ccagcttttt cagccggtgt agctgtttgg acatttgtca cgatgacacc
    41461 gttagttaca gatgaaggaa tcttcaagat ttgttcttgt tgctgagtag aaacggcaga
    41521 taagtcgacc attgtgatac ctagagctgg acgtgtcact tttccgtctt tttccagttg
    41581 attgatgata ttcactacat cgttacttgg aatagcaaat cccattcctt ctacgcttac
    41641 attagaagta gattcagaag tacttgcgat cttacttgag ttgataccga taacttgtcc
    41701 ttcgatatta actaaaggac caccagaatt tccagggttg attgctgcat ctgtttggat
    41761 cgcattgata ttgactgttt cgttcgattc attcgtactt gtcacttgtc ggttcaagga
    41821 agaaatgatt cctgaagtga cagaatttgc atattcagat cctagtgggg aaccaatcgc
    41881 aatcgcaggc tcaccaacct tcagagcact tgagtcacca aaagaggcaa ctgtttcgac
    41941 tttgtctgaa ttgatcttca acaccgctaa atctgaataa gcatctgttc caactaattc
    42001 agcttttacc ttcgtaccgt ctttcatcaa gacttcaaga ccttgctggc catctactac
    42061 gtgattattc gtgactacat aagcagtatt cccgtctttt ttgtagatca cgccgcttcc
    42121 ttcactataa gcttccaaat tactatcatc tgagctgcta ctttcttctt gctgcccgaa
    42181 tagtccgcca aaaccgcttg attggttttg actttgtaag ttgataacag aaacgacagc
    42241 gtcttgaact ttatctaccg ctttggtgat atcagaatct acattgactt tgacattttc
    42301 cacaactgtt tcaccggcag aattctgatt gccgcttgta gcagtagaac tattgttgcc
    42361 agtgcccatc gctgcataaa aaatgccagc tgtcagtagt cctccaacga tccctccaac
    42421 aagccccaga cctaacttac gccagaggct attattttta ctttttttca tctttggtgt
    42481 cacatttttt ctatccatgt gttcctcctc caattacatt catttatcta ctgttagtat
    42541 agtcttattt gttgacttta gtctactttg agactgtgaa ctttttatga aaaactttag
    42601 gaaaaatcat tgttttcaga tgaaaatatt tgattttctt tttccacaga tactgtgtaa
    42661 aactacgcgg ataggattgg aaaaataagg gataaaacat atccacagcc tgtgtataaa
    42721 gtggataaat tcttataaaa cgtatgtttg cctaatgaat gtgatgaaat aaggtaaaat
    42781 aacactgttt tctgtggata aacctgtgga tgttgtgcat aactgttggg gaaatctttt
    42841 taacaaaaat agccaaaata aacatataga cgaaaaaatt gattataata aacaaagtga
    42901 gcatgaagac caaaggagag aaaacatgaa tatcaaaatt atctcagtag gaaaattaaa
    42961 agaaaaatac ctgatccaag gtatcaatga gtatgtcaaa cggctgaatg cctatgcaaa
    43021 aatcgaattg atcgaagttc ctgacgaaaa agccccggag aatttaagcg aagcgcaaat
    43081 gagacaagta aaagaaaaag aaggggaacg gatcttagcg aaaatcaaag aacaagaata
    43141 cgtctatgcc ttagcaattg aagggaaaaa tcctacaagt gaatcttttg ctaaacaaat
    43201 cgaccaatta ggtatccaag gaaaaagcca ccttgttttt gttatcggcg gctctttagg
    43261 actcagcgaa gccgtcatga aaagaagcaa tgctcagatc tcttttggaa aaatgacgta
    43321 tccacatcaa ttgatgcgtc tgatccttgt cgagcaaatc taccgtgcct tccggatcaa
    43381 tgcgggggcg ccttatcata agtgatgcgg tttttatcat tgatcctaaa aatcttagca
    43441 agacaaaaag tacgtaagaa agtaaagact aaatacttcg gaggaacaaa tgaataacaa
    43501 tgataggtta ttgcgattgc gttatgcgat tgatttgaaa gatacggatt tgattaaagc
    43561 ttttgagcta ggcggtgtac cactgactaa agaagaagct caggcggtac tgaccaaagt
    43621 acaggataag aacaaagata atgcagaaaa taatgtgtat gaaaaaacca tcaacaacca
    43681 agtttttgac gcattcctaa atggcctgat tctcttggcg cgtggtccgc aaaaagataa
    43741 gccgatagta gacacgccgc aaaaaagcaa ggaaatcaaa tatatcaaca atgtgttgct
    43801 aaaaaaatta aaaatcgcct tgtctatgac cactgatgat attttagacg tttttgctga
    43861 ggcagaaatc tatccatcaa aaggcgaaat cggggcattt ttgagaaaag aaggccaacg
    43921 aaattttaag ccttgcggag ataaatatat gcgtaatttt ctcaaggggt taggaattta
    43981 taatcggaga aaagtataaa gaaatttcat gagaaatcaa aacaatggtt gttttgatga
    44041 gagctagttt ttgtatgaga ttaaaatatt atcttaatcg aggagctgga ttggttttat
    44101 gaatgatgaa tataagaatg atgaagataa gatgcttttt gaagagatag agaatcgatg
    44161 taggctcaat tttgagcttc ggggaaagat gtctttaata caacaaaaaa agtatttagc
    44221 gaataaatca gagtttactc tgggacatgt tgaaaaatta atctcggatt ggattagtag
    44281 tagaagcgaa tttacaaaaa taaaacagcc gattaaattt gacatgaaaa aacttttgtt
    44341 aaataagagt gagataggta atagagatca atatattaga gctaaagggc aagaaataat
    44401 tgattcatta ggtgaaatga ggtcatacaa ttatttgtat gtaacacata gagctgatgg
    44461 aatggtcatt actgtgggga aatcttcttc taatgatatt tttttagatg gagatctttt
    44521 ttatcaactc aatataaatc acttgtctgg cactgaaaat ataattttaa ggacggaata
    44581 tggcaatgaa atatttgcaa aatatgatga gattttaaaa aattatttag actgggcttg
    44641 gataattccg gttgagtcag gagatgctaa gaaactggag agattattgg gcgatgaact
    44701 aattaataaa aaagtagcaa ttttaaatta ttattcccat agacaataaa aggctatttt
    44761 tcatgataat actagttata actgtaaaaa tggaagaaaa tctgggaaag aagtttttaa
    44821 ataagtgtat tgttcgattt taataaataa taagcaatac catccgtctg tgttgcgcaa
    44881 catcgtacta caatgaatat tttttcacaa aaagaaaatc atccatcgaa tgaatggaag
    44941 gttttagtat acacgaggtt aatgccccct cttttataat cagaggagtg agattttttc
    45001 tatgaatgtt tctattttct tattgctagt ctagtttctc ttacgatgaa attgggattc
    45061 tattccagtt tttattgatt tgctgataaa ataacttgaa tggtggatat aaacatgata
    45121 aataagagaa gaataacaaa acgaggttgt gaaaagattc tcaaatgagt ataatagaaa
    45181 gaaaaaggag gaagaatcaa tgaaaatagc aattgcaggc gctggcgcaa tgggaagtcg
    45241 ctttgggttg atgcttcacc aaagtgggaa cgaagtatta ttaatcgatg gctgggcaga
    45301 acatgtccaa caaatcaaag aacatggatt acaagcaaat tttaatggta aagaggtaga
    45361 agcaaaacta ccaatcgttc ttcaatccga agtagaaaaa gaagatcaag ttgatctgat
    45421 tattctattt accaaagcga tgcagctgga aaaaatgctg caggatatcc aatcattaat
    45481 caaaaaagat acagaggtct tatgtctatt aaatggtatc gggcatgaag atattattga
    45541 gaaatttgta ccaatggaaa atatctatat tggaaatacc atgtggacgg caggtcttga
    45601 aggtcctggt caggtcaaat tatttggaag tggttcagta gaattacaaa atttaggtga
    45661 tggaaaagaa gcagctgcaa aaaaattagc agacaaattg tctgaatcag gattaaatgc
    45721 tcatttttct gacaatattc actattctat ttatcgcaaa gcatgtgtga acggaacaat
    45781 gaacggacta tgcacaatct tagacgttaa tatggctgag ctaggaaaaa catcgactgc
    45841 tcataaaatg gtggcgacga ttgtcaatga gtttgccaaa gtagcagcag tagagaagat
    45901 tgaactagat gtcccagaag tcattgcaca ttgtgaatct tgttttgacc cggaaacaat
    45961 tgggttacat tatccttcaa tgtatcaaga cttgattaag aaccatcgat taacagaaat
    46021 cgattatatc aatggcgcaa tttctagaaa agggaagaaa tatggtgttg cgacgcctta
    46081 ttgtgatttc ttgactgagc ttgtccatgc aaaagaagat agtttgaacg taaaataaag
    46141 gagagattga atggaaactt cacaaaaaat atcaccgaaa atttttctga ataaagtcct
    46201 cgctggtaca gcaacaggaa tcattattgg attgattcct aatgcagtat taggtgccat
    46261 tttaaaatat tttagtgcgt atccctttgc ggtaacgatc gcacagttag cagttatttt
    46321 tcaactagca acacctttga tcattggtgg tttgattgcc ttacaatttg gatttgatcc
    46381 gatgagaatg atggttgtag ccggagcttc ttttgttggt tctggtgtag tcaaattcac
    46441 acctgatttg ggtgaaacgg gtgtttacgt tggtgctggt acaggcgata ttattaatac
    46501 gatgctgaca gcatcgattg ctgtcgggct gattttactg attggaaata gttttgggtc
    46561 agtgtcgatt gtgttaacac cgattgttgt aggtgtaggt gcaggtttac ttggttttta
    46621 tttattccct tttgtaacag caattacttc tgcaattgga tcattgatta ataacttcac
    46681 taccttacaa ccgctgttaa tgtctatctt gattggttgt tcctttgcct ttttgattat
    46741 ctctcctatt tcaacagtag caatcggaat ggcaatccaa ctagatggtg tttctgctgg
    46801 tgcggctgcg atgggcgtag ctgcaaccac ttttgtttta gtagtgaact cttggaaggt
    46861 taataagtca ggtgtgacaa ttgcgattgc tttaggcgct atgaagatga tgatgcctaa
    46921 tttatttaga aaacctgtga ttcttattcc ttgtttagtt agtgcagtga ttacagctat
    46981 tcctgtcgct ctattttcta tttcaggtac gcctgcttct gctggtttcg gcgtggtcgg
    47041 acttgttggt ccgttggctt ctttagatgc agggctaaat ggctttcttg ttgttctttg
    47101 ttggttagtc atccctgttt ttgcggcact aattactcaa tttttatgcg agaaagtatt
    47161 taaactgtat gatcgagaag aagtcttcaa atttttaggc tagtagattt tactaagaaa
    47221 ttttcaatca atggatgcgt aagtgagatg tcaacaacat ctgacttacg tttttttgcc
    47281 tttttgagta ggagattgat gctgacaact gaactcttca gtgccattcg attttttaaa
    47341 atgtagaaga aactcacctt tatgttattg attgttcaaa aggcaactag aataaaaata
    47401 tgtgttaaaa tgagaaaaaa tttgattgtt ggatgtatcg aataatcact aaaaagtagg
    47461 ggaagaaaat gatttttttg caaaaatatg gtttttattt tttattatta ggtgtgatta
    47521 gtgatttatc gacaccgtat atccttggcc ttttttatcc caaactgaat caaatgacta
    47581 ccgtaattag tgtgtttggt gacgtagata gtccagtcag acgtgctttc ttggtctggt
    47641 cagttgtatc tggacttttt tttgttcttt cgttgcctgc gttgtatcac ctatttgttg
    47701 gaacatcaaa aacacttgca atacttgttg ttgcaacagt tggtttatat gggataggtg
    47761 attgtatttt tactggctta ttcagtatca atacgaatga aagctcttgg aatctatcga
    47821 cttggatcca taatataggt tctgggctag gctatgcagg ctttctactt ttcccattgt
    47881 tacttgttct gctttatcgt caaagtggtc aaggtacgct tagccatttt tatcttgttt
    47941 taacagttat tagtttacta attgcaggtc tttacggatt agcacggatt ccgtcgatta
    48001 gtcagtttgc cttctttaag caattgggct tctttcaacg attaagtttt ttctttaact
    48061 atttgccgat gatttgttta agtttttttc aactacgaaa gtagaaaaag cagtgtgaag
    48121 ctgattaagt ggttctatga tatttggtgt tgttactcaa ggcgagtaag gacaccaaat
    48181 atcatagaac ccttcttttt ttgtatagat aaactgcttc tatcactgat catcgatgtt
    48241 gtctaactga tgtcgccgaa tgcaagcatt acctttgtca gattaaaaaa tactcgttac
    48301 acttaatata gaacgacgaa agaaaggatg atctcaatga aaaaaatgta cgaaacagca
    48361 atgatcaatc gtggaggccg tgatggcgaa gttgaggcac caaacggaag tatgcacatg
    48421 aagatcgacc gcccaggaat ccatagtgaa gggacaaatc cggaacaact ttttgcggct
    48481 ggatacgcat cttgcttcaa cggcgcggtt caacacatgc tggaagaaaa taatcttgag
    48541 tctgattctg aagtcaaagc acgggtatct ttatttcaat tagaagatgg gggctaccaa
    48601 atcggggtcg ttttagaagt ttctttgcca ggacttgaga aggaccaagc agagaaaatc
    48661 gcagaagaag cccacgcatt ttgcccatac tctaaagcaa caagaggcaa tattgatgtg
    48721 gaagtgacta tagtagaata agggattctt aatgttacta taaatgagat tatgaaagat
    48781 cctataaaga aacgctagta aaagaaacat gtagttacgg taattaatag cggtatatgg
    48841 acgattgatc gaatagaaca aggaggcaaa aaaatgacac agcgggtagc aataatgatt
    48901 ttagtgttgc tggtcattgg cctccttgtt tactatgtcc taaagtttaa gcattggaag
    48961 cagcaacgga tacatcagga tattgaaaaa aagttaaaac ggtatcctat tgttcaagca
    49021 gcttgggaaa aggctgaagc aaaacaatat aacattcctg gattaacaga aacaagaatg
    49081 gtggtgccgg aaaccggcga aaatgaggtt tgtcaatgga tgacgccgca agggttggct
    49141 ttttctcaag attttgtgtt tatttctgcc tactgctatg accatcagca tcattctatt
    49201 attcatgtac tggatagaga aacaggacag ccgatcaaac tgctgatttt acctaaacgg
    49261 ccacatgtag ggggcttagt atatgataca aaacgggaac ttttatggtt gacaatcaca
    49321 ggatctgcta ctggacgagt agccgcttta cgtttgatcg atatcttagc agatacatct
    49381 gaagagacgg gacagccaat cgcttattgg ctgacaactg atctctcaga gattccacaa
    49441 gcatcgtatt tgactcaaaa caatgatcaa ctggtatctg gcaacttcac actaaaaggt
    49501 gaaggacagt tgactttcta tctattgccg acgatagctg aaatgagaac agcaattcgc
    49561 cgcaaagaca aaatccaacc aaccgtgaca atgagcagaa aaacatctga taagattcaa
    49621 ggtgtggctt tttatggcca ttatctctta gccagtcgtt cttatggtcc ttatacctcg
    49681 gaactcttag tgtccgagcg ggactctcct agccggatcc taaaacgaat caaatttcca
    49741 ccgtatttgg aacaaatcgt tgttgtagaa gatcgtctag cagtattgtt tgaaagtggc
    49801 gccgcagcgt atcgcgagaa agccaatcct gtgttagcca atgttttatt actagactta
    49861 gcaacgttgc ttcatgcaaa taagcgtccc aaaaagattg ctgcaactaa aaaatagtcg
    49921 gacaactcaa gatgatcact gttgttcata aagaacgaca agaaaaatca tctagcgatg
    49981 ttcaactatg ggtttattta ggaagtattt ctactaagat tcaatatggc tatacagatt
    50041 cggcgaaaaa aaaaggcaat gttaagtttt taagaatcac agatatacaa gagggacgtg
    50101 taaattggtc cagtgtgcct tactgtgata ttagtaattc taaactagtt gatttaagat
    50161 tggaagaaaa tgacattttg atagcaagga caggaggaac tatgggaaaa agtttcttgg
    50221 ttaaggaaat aagtgaggaa tctgtttttg cttcttactt gattcgaatt aggttagttg
    50281 agaaattatt atcagaatac gtagactgct tcttagactc gccattatat tggaagttat
    50341 tagaaaaaat ttcttacgga actgggcaac caaatgtaaa tggtacaaat ttatctaaac
    50401 tacttatacc attaccacct ttagaagaac aacaacgcat gactactaaa atagaaatga
    50461 taagaagatc tattcgtaga atcaattttg agtaaatata acggacctag catccatttt
    50521 ataccagatg atgctagg
//
LOCUS       DAFFSV010000007        47238 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFFSV010000007 DAFFSV010000000
VERSION     DAFFSV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.007"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(32..2107)
                     /locus_tag="LR110_000335"
     CDS             complement(32..2107)
                     /locus_tag="LR110_000335"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBL4821341.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDYEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTYHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDADRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2109..3026)
                     /gene="glyQ"
                     /locus_tag="LR110_000336"
     CDS             complement(2109..3026)
                     /gene="glyQ"
                     /locus_tag="LR110_000336"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBL4821342.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3412..4224)
                     /gene="recO"
                     /locus_tag="LR110_000337"
     CDS             complement(3412..4224)
                     /gene="recO"
                     /locus_tag="LR110_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBL4821343.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            complement(4367..5266)
                     /gene="era"
                     /locus_tag="LR110_000338"
     CDS             complement(4367..5266)
                     /gene="era"
                     /locus_tag="LR110_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBL4821344.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5281..5676)
                     /locus_tag="LR110_000339"
     CDS             complement(5281..5676)
                     /locus_tag="LR110_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBL4821345.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            complement(5660..6136)
                     /gene="ybeY"
                     /locus_tag="LR110_000340"
     CDS             complement(5660..6136)
                     /gene="ybeY"
                     /locus_tag="LR110_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBL4821346.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6165..8357)
                     /locus_tag="LR110_000341"
     CDS             complement(6165..8357)
                     /locus_tag="LR110_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBL4821347.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8381..9352)
                     /locus_tag="LR110_000342"
     CDS             complement(8381..9352)
                     /locus_tag="LR110_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBL4821348.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10058..11437
                     /gene="brnQ"
                     /locus_tag="LR110_000343"
     CDS             10058..11437
                     /gene="brnQ"
                     /locus_tag="LR110_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL4821349.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11593..12039)
                     /locus_tag="LR110_000344"
     CDS             complement(11593..12039)
                     /locus_tag="LR110_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBL4821350.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12066..12242)
                     /locus_tag="LR110_000345"
     CDS             complement(12066..12242)
                     /locus_tag="LR110_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBL4821351.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12404..12832)
                     /locus_tag="LR110_000346"
     CDS             complement(12404..12832)
                     /locus_tag="LR110_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL4821352.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12924..13784
                     /locus_tag="LR110_000347"
     CDS             12924..13784
                     /locus_tag="LR110_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBL4821353.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13879..14229)
                     /locus_tag="LR110_000348"
     CDS             complement(13879..14229)
                     /locus_tag="LR110_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL4821354.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14245..15972)
                     /locus_tag="LR110_000349"
     CDS             complement(14245..15972)
                     /locus_tag="LR110_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBL4821355.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            complement(16186..17112)
                     /gene="trxB"
                     /locus_tag="LR110_000350"
     CDS             complement(16186..17112)
                     /gene="trxB"
                     /locus_tag="LR110_000350"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBL4821356.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17257..18693)
                     /locus_tag="LR110_000351"
     CDS             complement(17257..18693)
                     /locus_tag="LR110_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL4821357.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18690..20093)
                     /locus_tag="LR110_000352"
     CDS             complement(18690..20093)
                     /locus_tag="LR110_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL4821358.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20323..21225
                     /locus_tag="LR110_000353"
     CDS             20323..21225
                     /locus_tag="LR110_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL4821359.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21266..23152)
                     /locus_tag="LR110_000354"
     CDS             complement(21266..23152)
                     /locus_tag="LR110_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL4821360.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23248..24099)
                     /locus_tag="LR110_000355"
     CDS             complement(23248..24099)
                     /locus_tag="LR110_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL4821361.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(24556..26340)
                     /locus_tag="LR110_000356"
     CDS             complement(24556..26340)
                     /locus_tag="LR110_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4821362.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26340..28091)
                     /locus_tag="LR110_000357"
     CDS             complement(26340..28091)
                     /locus_tag="LR110_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4821363.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(28233..28457)
                     /locus_tag="LR110_000358"
     CDS             complement(28233..28457)
                     /locus_tag="LR110_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBL4821364.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28708..30294
                     /locus_tag="LR110_000359"
     CDS             28708..30294
                     /locus_tag="LR110_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBL4821365.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            complement(30604..32379)
                     /locus_tag="LR110_000360"
     CDS             complement(30604..32379)
                     /locus_tag="LR110_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBL4821366.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(32590..33492)
                     /locus_tag="LR110_000361"
     CDS             complement(32590..33492)
                     /locus_tag="LR110_000361"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBL4821367.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            33771..36116
                     /locus_tag="LR110_000362"
     CDS             33771..36116
                     /locus_tag="LR110_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL4821368.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            complement(36279..36545)
                     /locus_tag="LR110_000363"
     CDS             complement(36279..36545)
                     /locus_tag="LR110_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821369.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(36538..36861)
                     /locus_tag="LR110_000364"
     CDS             complement(36538..36861)
                     /locus_tag="LR110_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821370.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            complement(36873..37079)
                     /locus_tag="LR110_000365"
     CDS             complement(36873..37079)
                     /locus_tag="LR110_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821371.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            complement(37076..38131)
                     /locus_tag="LR110_000366"
     CDS             complement(37076..38131)
                     /locus_tag="LR110_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HBL4821372.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQEL
                     NNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELEK
                     KWHGKKACGKHVLSPLR"
     gene            complement(38131..38526)
                     /locus_tag="LR110_000367"
     CDS             complement(38131..38526)
                     /locus_tag="LR110_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821373.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            complement(38569..38823)
                     /locus_tag="LR110_000368"
     CDS             complement(38569..38823)
                     /locus_tag="LR110_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821374.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            complement(38820..39215)
                     /locus_tag="LR110_000369"
     CDS             complement(38820..39215)
                     /locus_tag="LR110_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821375.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            complement(39561..40562)
                     /gene="ccpA"
                     /locus_tag="LR110_000370"
     CDS             complement(39561..40562)
                     /gene="ccpA"
                     /locus_tag="LR110_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBL4821376.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            40772..41875
                     /locus_tag="LR110_000371"
     CDS             40772..41875
                     /locus_tag="LR110_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL4821377.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            complement(41937..42539)
                     /locus_tag="LR110_000372"
     CDS             complement(41937..42539)
                     /locus_tag="LR110_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL4821378.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(42542..42982)
                     /locus_tag="LR110_000373"
     CDS             complement(42542..42982)
                     /locus_tag="LR110_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBL4821379.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(43108..44046)
                     /gene="galU"
                     /locus_tag="LR110_000374"
     CDS             complement(43108..44046)
                     /gene="galU"
                     /locus_tag="LR110_000374"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL4821380.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(44061..45086)
                     /locus_tag="LR110_000375"
     CDS             complement(44061..45086)
                     /locus_tag="LR110_000375"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL4821381.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(45130..45960)
                     /locus_tag="LR110_000376"
     CDS             complement(45130..45960)
                     /locus_tag="LR110_000376"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL4821382.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(45975..46913)
                     /locus_tag="LR110_000377"
     CDS             complement(45975..46913)
                     /locus_tag="LR110_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBL4821383.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            complement(47050..>47238)
                     /locus_tag="LR110_000378"
     CDS             complement(47050..>47238)
                     /locus_tag="LR110_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL4821384.1"
                     /translation="NSNGILRRNGLPKSMDFREVNQTFISSVSNQRNYIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT        13174 a        10076 c         8458 g        15530 t
ORIGIN      
        1 cctgttctct ggtttattta catattagaa attattttgt gatgatttga tctaaacttg
       61 ctagtgacaa agccatggca gcaatttgac ttaattgcgt taatcggttc ttacgtactg
      121 ctggttcttc tgccatgacc atcgtctgat caaaataatt ttcgatcagt gggcgaaggc
      181 ttacaagctg atcataattt tcgttgatcg tttggtcagc aaagcttgtt ttcattgcct
      241 ctactgcttt gtgcagttct ttctccgctt cgttttcgaa cagttcaggg tctacttctg
      301 attgtttttc aagctccgct tttttcgcta agttcacgac acgtgtcaaa gcttccatcg
      361 atggtttgaa gtcttcatct gcaagatgct gcttgaacaa ttgtgctgct gcaaatactt
      421 ttgtcagatc tttttgttct gcattcaaaa cagcttcgat gacatcatga cgaatattct
      481 tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacctgt tgttgtcccg
      541 ctgacaagcc aatgccaaaa cgatccgcat ctttactgat aacttcacta atcgtttctt
      601 gcagaacaga taaaggaaag gcccattttt tcgattcgat gattcgaacg acaccatatg
      661 cttgacggcg caaagcatat ggatcgtttg agccacttgg gatcatccca actgcaaaga
      721 aagaaaagat actatctaat ttatcagcaa tcgctaaaac agaacctact gtagtctcag
      781 gtagatctcc ttcactggat ataggcatgt aatgttcacg gattgctgtt gctacggctg
      841 gtttctcgcc tttaagcaga gcatattttt ctcccatgat cccttgtagt tcagggaatt
      901 ctcctaccat gttggttact agatcaaatt tatagatttc agcagcgcgt tgcagatcca
      961 tcaattcttc ctcagatagt cctacttgct cgcctatgat gcgggcgatg acttgtaccc
     1021 gttgcatttt ctcgtagatg ctaccgattt tttcatggta agtaacagtt tttaattttt
     1081 caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc gcatcttcta
     1141 aacgagcagt caatactttt tgattgcctc gaaccacatt ttccaatttt acccgatcac
     1201 cattacgagc agaaataaaa tgaggaagca gcataccttg atcattccgt acctcgaaat
     1261 aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagaa agatattttt
     1321 cttcgtagtc gccagaaaag acagttggat attcgactaa attgttgact tcttctagta
     1381 aatcttcgtc tagatcaata acccagtgat ttttttctgc taattcgttt gcttgtgtaa
     1441 cgatcatttc ttgtcttttt tgcggagacg caatgacgaa ttgttcattc aatttttctt
     1501 catattcttt tgcatgcgca aatgtcacat cttcgcctaa gaaacgatga cctcgagagg
     1561 ctctgcctgt tttgacatcc aagatttcga atgggattac ttcatcatct aacaatgcca
     1621 cgatccagtg aatagggcgg atatattcaa aatcgtattc tgcccaatgc atcgttacag
     1681 ggaaagtcag gcttttaatg acatctgcta aacctgtcaa tacttctttg gattccttac
     1741 catgagtgta tttcgttaca tagacatatt ccacaccttt taattctcta aaagtgatag
     1801 cgtctgtcgt cagaccttgt ccgcggacaa atccttccgc tgctttcgtc cagtttccat
     1861 ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcactg tccgcttgtc
     1921 gttctggaat atttgttact ttgattgcta aacggcgcgg tgtggaaaat gtttcgatcg
     1981 tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct aattgtttca
     2041 tgcttggtgt gacaacatgg gcaggcattt cttccagccc gatttcaagt aataagtctt
     2101 ttgccatctt attcagcctc cttcacggtt tctggatttg ctgtgttttt attcaacaaa
     2161 gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg agccatgttg
     2221 cggatacggg aaagataacc ggcacgctct gttacggata ctgcaccgcg agcatctaat
     2281 aaattgaatg tatggctgca tttcaaaata taatcatacg ctggatgaac aagattttca
     2341 tcgatacagc gtttagcttc tttttcaaaa cgatcaaagt tgcttagtag caagtcttga
     2401 tcgcttactt caaaagaata tttggaatgt tcatattctg gttgtttgaa gatctcgcca
     2461 tatttcacgc catcagccca ttctaaatca tagacacttt caacttcttg gatataagaa
     2521 gcaagacgtt ctagtccata agtgatctca gaagtgactg gtttacaagg cagtccacca
     2581 acttgctgga agtacgtgaa ttgtgtgatt tccattccgt caagccatac ttcccaaccc
     2641 acaccggcac agcccatgga tgggttctcc cagttgtctt caacaaaacg gatgtcgtgt
     2701 tctaatggat cgattcctaa aaggcgcaag ctttctaaat aaagctcttg gatattctca
     2761 ggtgaaggtt tcatcacgac ttggaattga tgatgctgat acaaacggtt tgggttttca
     2821 ccataacgtc catcagctgg tcggcgagat ggttctacgt aagcagcatt ccatggttct
     2881 ggtccgattg cccgtaaaaa ggtatatggg ctcatcgtac ctgctccttt ttctgtgtca
     2941 tacgcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt taaaatcata
     3001 tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttaa agcgttgctg
     3061 tcaaataaga atacaggaaa gaaaacgaca aaaaatccct atgtctgatt acagacatag
     3121 ggacgaataa ttcgcggttc caccctactt ccgaataaag attcggcagc ttcatttatc
     3181 gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc tcccactctc ccgaactcgc
     3241 taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc agttttcttg
     3301 tttctataat agatcctctc actgaacgat ctatttgatc tacggttaat aatagacgga
     3361 actccctgtg tttgtcaaat ggttttgatt ttttgttgta tgtttcattt actattcttc
     3421 ttttttaggt agatcttttt cagacatgtc tttctctggc gtctttaata cgtgtgccca
     3481 agacttcatt tgatcgatga actttttgct tttcaaatga ataccgacat attcatcata
     3541 cagctggtcg atgagttcac ggattgcttg tttggtttct tctttcagat ggattccact
     3601 gattttatca taagaaatag ctgaaaacat ccgaacgaaa taaacagctc gaggatctgc
     3661 atgataacgg tgatcgtcca tcgaccaatg tctttcgcat atcacaccac catatttgga
     3721 agaatagtca aattttcctt ttgtttcccc acatacgacg cagtgtgtcc agattggtgc
     3781 aattccgaag cgttgcagta tttgaacttc aaagatattc gtgatgattt ctgcatcatc
     3841 tccttgatcc atccgctgca gagcttgttg cgtaaattgg aataaatgcg ggtcatattg
     3901 atgatcttcg attgctgcat ctactagatt tagcaaatag gtaccataac cgctcaaaaa
     3961 gatatcttct tggatgttcc gaaacgcatg gacgtctttg acactgctta aaaacgacaa
     4021 accttcactt cgaaaattac cgatatagac tgcttcggta aaggggagca aagccggagc
     4081 taatggctga ttttgccgat ggattccttt gatataaaac atcattttgc cagaagattc
     4141 cgtaaagatt ttgactaatt tatctttttc tttgtaatta cggctagata aaatcaagcc
     4201 tttcgattct gcaagctggc tcatattctc cctcctaaaa aataaatctg gaaaagcaga
     4261 ttctgccgat ccagattatt tatgtttcga tcttccgccg tttttctcaa cgtgcggatt
     4321 tttatccagt ttatttctat acttaaccta taagaagttc agaatatcag tattcatctt
     4381 ttcggtagcc gaagtcttgc agatagactt ttttgtctcg ccagtctttt tggactttga
     4441 cccaaagttc tagaaatact ttgtctccta aaagattttc gatatccttt cttgctttcg
     4501 ttcctacttg cttcagcatt tttccgcctt tgccgataat gattcctttt tggctatctc
     4561 tttcgacgat gatcgtggcc tggatatgga ttttatcatt ctcgtttcgc ttcatcgaat
     4621 caacaacgac tgcgacagaa tgaggcactt cgtctcgtgt taaaaacaag actttctcac
     4681 ggatcaattc agaaacgatg aagtattcag gatgatcagt cacttgatca tctggaaaat
     4741 attgtgggcc ctctggcatt tcactgacta atacatccat caaacgttcg acgttgtttc
     4801 cttctgtagc agaaattgga atcacttgtg caaagggcat ctgcgttgaa taatcttcga
     4861 tgattcccaa caactcgtct ggatggattt tatcgatttt attgataatc agataaacag
     4921 gagaatgcac gttttttaga cgttcaataa taaaatcgtc tccccggccg cgtttttgat
     4981 cagcgctgat cataaataag accgcgtcca cttctttcaa cgcactgtaa gctgcttcta
     5041 ccataaaatc tcctaaacga tgctttggtt tatggattcc tggtgtgtca ataaaaatca
     5101 actgagcttc tggtgtagta tatactcctt ggattttatt tctagttgtc tgcgccttat
     5161 cactcatgat ggcaattttt tgacccacga ttcgattcaa taatgtggat tttcctacat
     5221 tcgggcggcc gacgattgcg acaaaccctg atttatgttc tggcatatac gttctccttt
     5281 ttaattaaac aacaaatgat atattttagg gacaaataaa atcaacccaa cgatgacggc
     5341 aaaacaagcg gtcactaaaa ctgctccagc tgccatatct ttgatttttt tcccaatcgg
     5401 atgaaaatga aaatccgtaa acatgtctac gacattttca aaaacagtat tgatgatctc
     5461 tactatccat acgagaaaaa cagctaaaag cagccataac cattctatcc gatcaagttg
     5521 gaaaatcacg cccataaaca aagcgaggat tccgaacgca acatgtttcc tcatgttgcg
     5581 ttcttcctca aaaacagttt tgactccttg gacggcaaat tccacggaca tcaggaaatg
     5641 tttgtttttc tctacgtttt tatcttttaa gtccataggc atctaagatc tctttctgta
     5701 aaccaaacat ttctttctca tcctctggtg tcatatggtc atacccgttg atatgcaaga
     5761 atccatgaac cgctaaaaag ccaagttccc gatcatacga atggccgtaa gcttctgctt
     5821 gctctgcagc acgttcagta gagatcatga tgtctcccag tacacgaggc aatggattct
     5881 ctccatcttc aaaaatcact gggatttcgt cttcaccctc ttcttccaag gcaaaactga
     5941 tgacgtctgt cggtgcatct tttccacggt aatcacgatt gattacttgg atcgctgcgt
     6001 tgtccatgaa agtaacagac atttctgttt cttctggcaa ctctaaatat gatgctgcaa
     6061 attgcaacaa gtcgtcgatt tctttcattt tctcttctga gacttggttt gtttcatcca
     6121 tgaaggtaat atccattata catcgcttct ttctattctt cttgctattc ttcttgttct
     6181 tcttttatct tctcaacttc cgatttcatt ttaggatatt tgatccgaga atggaaggta
     6241 ctgcttaaac cgctgatcaa tgatttttct actttacgga tttcttttaa cgtcaatcca
     6301 ctgtcatcca attgtccatc ggaaatacgc tcttctataa gattatggac aaaactttcg
     6361 attttttcgc ttgttggatg gtccattgcc cgtactgccg cttcacaact gtcagctata
     6421 ttgacgattc ctgcttcacg gctttgaggt tttggtccag gataacggaa ttgctcttcc
     6481 gtgacttccg ggttgcgttc ctttgcctta acgtagaaat aacgcatcag cgtggttcca
     6541 tgatgctgtc tgcaaatatc gatcaccatt tgcggcatat tataatcttc caatattttg
     6601 gcaccatctg tcacatgtcc aaagataata tgtttactgt cttctggcaa taagaaattg
     6661 tgcggatttt ccgctcctga aggcaagttc tcaacgaaaa agctggcgtg tttgatcttg
     6721 ccgatatcat gataatagca agctactcga gttagtaaag aacgtccacc gatttctgct
     6781 acagcattag cacttagatt ggctaccatc atgctatgat gatacgtacc tggtgcttct
     6841 tctaacaact gtttcaataa agggtgatta ggattgctga gttcatttaa tacgatcaca
     6901 ctatcatcat tgaccatcaa ttcaatatag gggtgcagac ctatcgtcag caagaatgag
     6961 aacaatgcac ctgtgaatcc gcagactagc gttaaccaag tcgttgcatc cccaaagctc
     7021 attccttggt agataatcaa aatgacatcc atcatcacag ggaaaataat gacccacata
     7081 gcagcgaata acccttgttc tgagatccgt ttgcgtctga cgatcgttgc catgagacca
     7141 gaaaatacat aggacatcaa aataactgtc agaaagttcg tgccaatcga atcataaaaa
     7201 atgaaaagtg cggatactgc ttggaaaata ccagccatga tccctgctct tcgattcaag
     7261 aaaaagctta aaacaagtgg agcaaaagcc gcggggtaaa acagcggaat ataagcagct
     7321 tgttctgttt ggaagagttg gaaaaatttc attagtacta cgctgactga cattgctgtc
     7381 acgtagaaaa gtacgtattt tgttctttct cctgcatttt gaaattgaag tgaattataa
     7441 atcagtacac cgatttgaag caaaacagct aaaaccatgg caatgagtgg gaaaatcgaa
     7501 gtcgtttgat tcgtcaatcc tagtaattcc aattttttca tcgctgaagc atcaatttga
     7561 tttccttcgc gaacaatgat ttctccttgg aagatcatca ctggttgtac agcttctcgt
     7621 gcagattgtt ttaattcttc tgtctttttc tcattcaata catcattgac tacaatgcct
     7681 tgatccacta agtagcggat catttgttgc tgctctgttg tgacattcaa atactgaatc
     7741 tgatcttctg cttcttgttt aaaagcttcc aattcgcttt cacgaacttg tttactcatc
     7801 tgttcgtcaa tgagcttcaa actttcatcc ctaaccgtgt gaatttccgt ttcagtcatt
     7861 tcaaatatcg tcgaataaaa attgttcggc agtttttggt aaaacgcaac gttttctgca
     7921 ttcacgtttt caaaattttt cttcagtgta gcaatccgct cttctgtagt aggtttcggg
     7981 atattttcat cttccttcgc cttatctacc ttgtcattat aagatttgtt gatggtatca
     8041 ttcgtcttat cgatcaattc gaataattgc ttgatccgat tgttctggtc atctgccaga
     8101 tctttttgat aagtatattc tggagtcact gcttctgctg ctagttttct tctttgttcg
     8161 gtttcttccg tgttctcgat cgttttattc gcacggatac tctcttctgc tacttggcct
     8221 tctctaaaat cattggcctt ttgtttgaca ctactaaatg taacgccgca aacaatgata
     8281 aaaaacaaga acagaaggaa aggcaatgct gcctttccga gtttttcttg tagtttctgc
     8341 agtgttttat tcaacatgtg cttatcctcc taagcaaagc ttattcttga tgaagatccg
     8401 ccttttcata agctttgatg atttcggcaa ctacagggtg ccgaacaacg tcacttgctt
     8461 caaaattaac aaaatcgatt tttttgattt gctttagtgt tcgttctgca tgaacaagac
     8521 cacttgtcgt ccctcgaggc aagtctatct gacttgtatc cccattgaca atcatttttg
     8581 attgataacc gagacgggtc aagaacattt tcatctgagc gactgtcgtg ttttgtgctt
     8641 cgtccaagat aacgaatgca tcatccaatg tccgcccccg catgtaagct aatggtgcga
     8701 tttcgattac gcctcgttcc atcaatcggt tcgtatgatc caatccaaaa atttggtata
     8761 aagcatcata aacaggccgc agataaggat ccactttttc tttcagatcc cctggtagaa
     8821 agcctaggtt ttcccctgcc tctacagcgg gccgcgtcaa aatgattttt tgcacttcgc
     8881 ctttttttaa ggcagcaatt gctaatacta cggcaagaaa cgttttccct gttccggctg
     8941 gccctacacc aaatacgatg tcatgtttgg ctaccgattc aacatatttc ttttgaccgc
     9001 tgttttttac ccgtataggt tttccattac ggtctttgac gatctcttcc tcatacatct
     9061 caacaaaata atctaatgta cctttttgcc ccattttcaa tgccgtcaca acatcgggcg
     9121 tacttacgtg gattcctcgt ttgatcaatt cctgtaatgt ccgaatgact gaggcagcca
     9181 aggaaatctg ggattgttca ccaatgatct gaatgatttc tccacgtgta tggatggttg
     9241 tttccgttgt ttcctcgatc agttttatat gcttgtcgtg tgcacctaaa agcatgctga
     9301 catcatcagc agctgtcaat ctaatctcta aagaactaga ctcttccgtc aaaaatagac
     9361 atcccctttt tttcgattca ttctttccta ttttaccata ctcagaagaa actatatagc
     9421 aaataagaaa aggttgtaaa agagtcgttt aaccatacgt accaagaaac gttaaaaaat
     9481 agccgtttcc attcgctaaa ggtctcgttt tgataaaaag aatctgagaa tgtaagttat
     9541 atgaaagtgg agaacaaaat attccgtttc attcagcagg tattctctag gtatttttct
     9601 tgtttcaaaa aaagaaaaca aaatagttaa aatattcttg catttcaaaa aaaacaggtg
     9661 tattattacg tcaacaaata aacatacagc aaagcgtcga ggaaaacagt aggctattca
     9721 acgatttatt cccagtgagt catgtaagtg agaaatgaca ataaatcgaa tagacgaaca
     9781 tcatttccga gtcaatgtcg aaaacgtaga tcattgtggc tgcctccatt atcaggctag
     9841 cggataactt tatcaaaaag cgtccgtgaa tgaagaagct tttttaagcg aaaaaaggtg
     9901 gtaccgttct agaatctaga gcccttttcc caagtggaaa agggcttttt ttatatttta
     9961 aggtggtggt tttcatggaa tctgatatta atggcattac taattgctgc aacgtttata
    10021 tcgtcaatca aaatgataat tattggagga aaaatttatg gaagaaacaa aaacttattc
    10081 atggaaacaa ctattgatgg tcagttcact tatcttcggg atgttcttcg ggtcaggtaa
    10141 tctaatcttt cctgtccact taggtcaaat ggctggcaca aactgggtca gtgctggcgt
    10201 aggatttgcc atctcaggcg ctcttttccc attactagct atcttagctg tagtagtgac
    10261 aaaaagtgat ggtctgtatg acttagctaa acctgtcgga aaatggtatg ctgccttgtt
    10321 cttaatcctt gttcatttga cgatcggacc atttttcggt acaccacgga cagctgctac
    10381 tgcttttgaa atggctgcta aaccattttt gcctgaaaaa tataattcat taggtatgtt
    10441 tttattctct gctcttttct ttttacttgc ttatttcctt actgtccatc ccacacggtt
    10501 ggtcaaatac gtcggaaagt atctaaactc cttgtttctt gcattattag caatcatttt
    10561 tgtcgtttct ttattccacc caatgggaaa tctaaatcaa tctgctgcag atacttacca
    10621 aacaaacgct ttaacaaatg gtgtcctaga aggttataac acggttgatg cagtagcttt
    10681 gcttgcctta agtgtcactt ttgtccatgc agtccgtgac cttggtttca aagataaaaa
    10741 agtcacagaa ttaacagcaa aagctggtag cctgagcgtt gcgctagaag tcatgatcta
    10801 ttttggactt gttcttctcg gtgcgatgag tttgaataag atgtctttat cagaaaatgg
    10861 cggaactgca ctttctcaga ttactaattt ttatttaggt aacttcggcg ctagcttctt
    10921 aggagtgatg gtcacattag gtgtcttcac taccgctatg ggactcgttg tttcttttgc
    10981 acaagatttc cataaactgt tccctaaagt cagttatatg acatggctgc ggttaacgac
    11041 tttcgtctct tttgtcgttg caaatgctgg tttggacaat atcatccaat ggtctttacc
    11101 agtattgatg cttttgtacc cattgtcgct tgcgttgatc ttgctttcct taacagcaaa
    11161 atttttccaa aagacaccat ttgtctatca agtaacgatg ttgtttgctg ctgttcccgc
    11221 cgtattggat atgctggcaa attcgcctgc tttagtgagt caacaaagag ttgtagcaag
    11281 catgctcgaa ttttatcatc atcacgttcc ttttgctgca ttaggacttg gctggatggt
    11341 cccaacgtta cttggttacg ctggcagctt actcttctat tacgcctatc gcctatcagg
    11401 gtacaaacaa gaagcaaatg aactaccaga agaataacca gatcaaacac aatttaacat
    11461 aagaaatatt aaacaggaaa aaaccagcta gatgaaagaa tcctattctt tcgtctagct
    11521 attttttaga tttctttttt acgagcacaa aaaaactgcg gacaatgatt gtccgcagtt
    11581 taaatgataa aattaagatt gtaatagttc tttcacaatg gcatttactt ggtttccatc
    11641 tgcttttcct ttgactttag gcatgatcgc acccatgact ttgccaaatt ctttcattga
    11701 tgaagcacca gtttgtgtga ttgcttcttg aacaagttgt cgaatttctt cgtcggaaag
    11761 ttgttccggc atgtattttt cgactatcac aatttcactt tttacttttt cagctaaatc
    11821 gtcacgtcct gcttcttcaa actcatgcaa agaatcacgt cgctgtttca tttcgcgaga
    11881 caacaccgtt aattcttctt cttctgtcaa gtcgtgacct tctttaatct gctcgttttg
    11941 tatagatgac ttgatcattc ggatgacttg taaagattcc ttatctttcg ctttcatcgc
    12001 tgttttcatg tcatcgttta atctacttag tagtgacaca ctaccaactc caattcatca
    12061 cagaactaga attttttgcg ttttctagct gcttcagatt ttttcttgcg tcttacactt
    12121 ggtttttcgt agaattcacg tttacgagat tcttgtaaag tacccgcttt tgacacggaa
    12181 cgtttgaagc gacgaagagc atcgtcaagt gattcgtttt tgcgaacgac tgtttttgac
    12241 atgttaattc cctccctccg agcttaaaaa gtaaccgtaa ttgttattga aatatatctc
    12301 aaaaaacaag actgtcctgt aaacattata tctaatgctg ttttgaccgt caaggaaatc
    12361 ttgctttttt ttaaaattat caaatgttat tttatttaaa tccttattga caattttcac
    12421 atcttccgaa aatctcgaat ctatgccctt cgatcgtaca tccaccgagc tgttcttcga
    12481 aataattcat tggacacata tggatttctt tcgttgttcc gcacaccgta cagataaagt
    12541 gatggtgatg gccgatttcc ccttgacagc aatgaaaacg aaacttcatc tctccattta
    12601 aatcggtttc ttccagtaaa tcgatttctg caaaatcatg gagatttcga taaatcgtat
    12661 cgtaacttac tcctttgaat tcctgattca tatagtcaaa aacttcttta gcggatacgt
    12721 agcgattgcg cttcactaaa taagttaaca atgcttctct ttttttagta tatttaaaac
    12781 cgttattttt caatgtttct agtgattgtt cgatcaatgt ctgttttttc atgacttatt
    12841 cctccaaatc aaaatgatta cgaagtacac attctagagt atggtataat aaacataaaa
    12901 aaaatgcaag tgaggtttta tccatgtctt ccgattctac agaaatcgtt actttttttg
    12961 tcatctccga ctctgctgga gaaactgcga caaaattagc gcaagccact atggcccaat
    13021 atcctagcgt tgagttcaat ttattccgca gaacatttgt gacagatgag aaaacattga
    13081 tgcaggcact gcaagatgca ctagaagaaa aagctcttgt cttacacaca ttgatcaatc
    13141 aagaattgat cgagatgacg cgttcttttt gtgaagaaca tcaattattt agttttgatg
    13201 tgatgactcc gccgatatca gaaatcgagc gcttaactgg tatcaagccc attcggcaac
    13261 ctggcgccct tcatttattg aatgagaact atttcaaacg gattaaagca atggaatttg
    13321 ctgtaaaata cgatgacggg aaagatcctc gtgggtttct agaagcagat gtcgttttgc
    13381 taggcgtatc ccgaacttcc aaaacgcctt tgagcttgtt tttagctaac aaaaatttaa
    13441 aagttgccaa tcttcctttg atcccacaag cacatattcc caaacagctt tgggagattg
    13501 atcctaaaaa aatcgtggga ttgacgaata acccagatat tttaaacaat atccgtaaag
    13561 aacggatgcg ctcttacgga ttaaatcctg atactgctta ttctgatatc gaaaaaatac
    13621 gagctgaact cgattttgca aatgatcttt atgaaaaact gggctgcgta gtaatcaatg
    13681 tcgcttcttt atctattgaa gaaacagctt cgcttatttt gaatgcttta gacctagaag
    13741 atcatagtta ttatggaact gaaaccaccg aagaaacaga ctaacagttt ttcttgtctt
    13801 tttcttaaaa aaaactggac tgtgtcagcg aagaagattc gttgacacag tccagtttgt
    13861 ctctatttgc acctatcgtt attgatgttc cacatgtttg agtgtctggt tcaaaatatc
    13921 cagtacgtga gaatcatcaa gactatacaa aatcgctttt ccctcacgat gggacttgac
    13981 gacatggttt tctcttaata aacgcagttg atgcgataca gcagattgtt ccatttccac
    14041 tgcttgggtg attgctgtca cattttgttc cccgtctttc agatacagca gaattttgag
    14101 acgagtgggg tcactcaaca ttttgaatag ttggctgact ttctctactt ttgttttttg
    14161 cgtgtctggc aatccattca cctccaactt gaaaaaagac tgacttctaa agtttaccac
    14221 tttttccatc agtctcaagt ttttttattc ttctgtcatc ttttggataa tcgcttcata
    14281 agcggctact ttttcagctg cgtcttctga agagtttcct tttacaccga tgtaaaattt
    14341 gattttcggc tcagttcctg aaggacggat agcgatccag ccattttctt ccagcgtata
    14401 cttcaataca tcagatggcg gtgtcgttag tttttctacg tttccttcac ggtctgttct
    14461 cgtcaactct ttgaagtctt ctgtttggat gacttttgct ccggcaaatt ctgttggtgc
    14521 ttcttcacgg aatttttcca tcaaagcaga gattttcgca gaaccttctt gtccgctcat
    14581 tgtcacagaa atcgtttttt ctgcgtagta gccataggtt tcaaaaatcg tttgcaagcc
    14641 gtcatacaac gttttgcctt gttttttata gtaagctgct acttctgcta ataagagcaa
    14701 tgcttggatc gcgtctttgt cgcgaacgaa aggtttaact aaataaccgt aactttcttc
    14761 gaatccgaac ataaaagtat gggaatgatc ttcttcaaat tgttgaattt tttcagcaat
    14821 gaatttgaat cctgtcaata cattgaacat ggtagcccca tagtgttccg caattgctgt
    14881 aggtaattcg ctagaaacga tcgatttcaa taccgctgcg ttttcaggta aagtgcctcg
    14941 cgattggtgt gcttccaaaa tatattgagc aaacagtgct ccgatttggt ttccggtcaa
    15001 tacttcatag gagccatttg gcaaacgaac ggctgcacct aaacggtctg catcaggatc
    15061 tgttgcaatc agcaaatcag cgccttcttt ctcgccaagt cgtatcgcat actcaaaagc
    15121 agagtgttct tctggatttg gtgatttgac tgtagtgaaa tctgggtctg cgacagcttg
    15181 ttctggaact agtacgaatt gttcgaatcc agcttgtttc aacgcacgtt cgcccaacat
    15241 ttttcctgtt ccgtgaagtg gtgtgtacac aagtttcaaa tctttcccca ttgtttccac
    15301 caattcatga tcaatcgtta ctgctttgat ttcttctaga taagcggcat ccacttcttc
    15361 tccgatgatc gtgatcaatc cgctatgttc agcttcttcg tcagataata cttcaacttt
    15421 taacggattg ctgacttcac ggacatattt cgtcaatgca tctgcgtctg caggaggcat
    15481 ttgtccccca tcttcgccgt agactttata tccgttgtaa gctgctggat tgtgggatgc
    15541 agttaccatg attcccgcaa acgtcttcaa atgacggact gcaaaagaaa gctctggtgt
    15601 tggacgtaga ctttcgaata cataagatgg gatattgtga tgtgctaatg ttttggctgc
    15661 ttccatggca aattctggtg attgatggcg tgagtcataa gcgatagcga cacctcggcg
    15721 ttttgtttct tcgccttgtg tatccatgaa acgtgcaagt ccttctgttg cttgtcttac
    15781 cgtatagatg ttcattcgat tgatcccagg ccctaagatt ccccgcatgc ctgcagtacc
    15841 gaattccaac ggtgcataaa aagcatcttc aagtttttca ggatcttcac ccagatcatt
    15901 taattgtttt tttaagtttt cttctaaatt ttcttcatgt acccattgtt catacacttg
    15961 ttcccaagac atgaaaataa cacctctttc tgaaatatgg ttcttaataa gtatagcatt
    16021 cacttattag atataaaagt atgaggacca aaaaacgctt aaatcaagaa aggattcttt
    16081 tatcaagcac ggttgcaagt attcgcataa atcggattca gtagatttca cgcgaaaaaa
    16141 aagagtaaaa gaacaattga aaccgttctt ccactctttt taaacttatg cagttgtttc
    16201 ttctaaagat tccaagtatt tatacacttg ttgtccagcg atcccaccat caccaacagc
    16261 agtagtgatt tgtcgcaaat ctttttcacg aacatctccg atggcaaaaa cgcctggtat
    16321 attcgtcttc atttcgttgt ctgttggaat ccagccagct tcgttcgtca accctgcttc
    16381 tctaaatggt gcagttaatg gatccagtcc aacatagatg aatactccgt ctgctgccaa
    16441 atcaaactca ttgccggttt tgacattacg tgctttcaca cctgtcacta ccatttcatt
    16501 cccaacgatt tcgtcaacga ctgtatccca cataaaggaa attttttcat tcgcaaatgc
    16561 acggtcttgg atgatctttt gtgcacgtaa ttcatctcta cggtggacga tgactacttc
    16621 agaagcaaat tgtgtcaaat agatggcttc ttctactgct gagtctcctc ctccgatcac
    16681 tactagacgt ttgttacgga aaaacgcacc atcacaaact gcacagtaag aaacgccacg
    16741 tccagcaaat tcttcctcgc caggtacgcc taatttacgg tggatacagc ctgttgcgat
    16801 aatgactgtt tttccttcgt aagttttatc ttcacaaata acttctttgt ggctgccgtg
    16861 atccttgata tcttgcacga ttccataagc attttctgtc ccaaatcttg aaacaccttc
    16921 atacatctta taagctagat caggacccat gattgaatcg aaaccaggat agttttcgat
    16981 ttctgccgta ttgttcattt ggccgccagg ggcgccttgt tcgatcatca atacggataa
    17041 gtttgaacgt gaagcatata aagcagcagt catcccagca ggacctgcgc caataacgat
    17101 tacatcatac atatgtagat tctcctttac ttaatcaagt aagcatactt tacagccaat
    17161 gcgaaaaaaa gtctattgtt ctgcttgcgt ttctcgaata gctttattat actcgaaaaa
    17221 tccgctgaaa aacaatagac ttttataaat cattgatcaa tcattcgaaa gattttctcc
    17281 atttgaatca attacttttt tataccagtt gaatgatttc ttcttcgaac gggctaatgt
    17341 gccatttccc tcgttgtcac gatcgacata aatgaatccg taccttttct tcatctcacc
    17401 tgtaccagct gatactaaat caatacatcc ccaagtcgta tagcctaata gatcgactcc
    17461 atctaattct actgcatctt tcattgcttg gatatgtgct gccagatagt cgattcgata
    17521 atcatcttcc acataaccat tctcatcggg cgtatcgact gcacctaagc cgttttctac
    17581 aataaatagt ggtttttgat aacgatcata aatatcattc atcgttattc tcaagcctaa
    17641 tggatcaatt tgccaacccc attcacttgc ttccagatat ggatttttga cagaagcaaa
    17701 gatatttcct gcagtctgtt ccaagagttc gttgtcagta gtcgaaactc gagaagaata
    17761 ataagaaaag gagataaaat caacagtatg ttctttcagc aatgcggcat ccccaggttc
    17821 catttcgatc tgaatgccat ttcgttccat ttctttcaat gcataagcgg gatattctcc
    17881 tcgtgactgg acatcaatga agaaataatt ttctcgatct gattttctag aagcccaaac
    17941 atcttcagga ttgcaagtgt atggataata tgctcctgct gccagcatac agcctacttt
    18001 gttctccgga tctacttcat gagcaatctt cgttgcgatg gcacttgcaa ccaactcatg
    18061 atgtgccgct tgatatttga tttgttcttc attttctcct tcttcaaaat acagccctgc
    18121 gcccatgaat ggtgcatgaa ggatcatatt gatctcattg aatgtcaacc aatatttgac
    18181 aagtccttta tagcgattga agatgactcg gcacagattt tcataaaaac caacgagttc
    18241 tctggaacgc cacgcaccat attctttgat caaatgcatc ggacaatcaa aatgtgtaat
    18301 cgtcacaagc ggctcgatcc cgtacttttg acattcttta aagacatcct cataaaattt
    18361 caacccagat tcatttggct ctttttcatc gcccattgga aaaatacgac tccaagcaat
    18421 ggacatccga taagtcttga accccatttc tgcaaataaa gcaatatctt ccttataatg
    18481 atgatacata tcgatcgcaa tttttgctgg ataaaaatga ttttcatcaa attcaaacat
    18541 tttctttttt ccagtaatga caggaaaacg gtcttctccg attggcacaa cgtctacgtt
    18601 cgctaaccca cgtccgcctt catcataacc gccctcgcat tgattggctg ctgtagctcc
    18661 gccccataaa aaatcttttc tgaatcccat catacttcct ccattacatg tgtattgctt
    18721 gataattctc ttgccagttc atcttggact tcgattcctt ctgcttttaa ccggctgatt
    18781 gtttccttga attgcggtaa atactgctga tgtgcaataa acaactcatc cattatccgt
    18841 tttgctgtct ttcctgatcg tataagcgga ttgatcgtaa acgcttgcaa agctgttcca
    18901 taatcaccag tcacggctgc ttcgatcgtc agcaattcca tattcttcat tacttgcaac
    18961 cagcctcttt cagcagcagg aagggaacca aacgctacat ttcgtgcgcc ttgtgcgcca
    19021 acataggcag tgacttctac tacacaatct gccggcagat caggaacagc tccatcattc
    19081 tttgtagata ccacgatctg tgtttcttta tttccataga tcgaagcaat cgtttcacag
    19141 gcagcatctg aataataagc accacctcgt ttttgcaatt gttcaggttt atgatccaat
    19201 tctggatctt tgtataattc aaacagctct gcttctgttt ctttcacttg ctgcgctctt
    19261 gtaccaatcg tttgatattc ttctaaagca tgttccagca tttcttcttc acggtagtaa
    19321 tagcggtggt agccacaagg aatcatgttc atttgtttta attgttcttt gaaaaacggg
    19381 atatcaaaga tatttttcgg caatccattg tcttcatcgt ataaacgatc aatcaattct
    19441 gccgtgcggt catttccttt attatctgct actttgtgcc aatggaagtg atttagaccg
    19501 gcaaacttgt agatcagctc ttcttttttc ttgccaagca ttgttggttc tgtcatcatc
    19561 gccattactg gcacattgca taatccgatc actttatccc atttgccata tctgataact
    19621 gcttctgtca ccatcccgct tgggttcgcg aaattgatca gccaagcatt tggacataaa
    19681 cgcttcatgt cttcaacgat tgccaaaacc actggaacgg tacgaaaagc tttgaatatc
    19741 ccgccagcac catttgtttc ttgcccaatc atcccataat aactagggat ccgctcatct
    19801 ttgattcgtg catctaattg accgacacga aactgagtcg taacaaaatc cgcatctttc
    19861 aatgcttctt cccgatccag agttgcatag attttgactt catacggaga agcatcccac
    19921 atacgctgag acatctgact gacgatctca agcttttctt ttccttcttc tacatctacg
    19981 agccagattt ctcgtatcgg taattcctca tatcttttga taaatccttc catcaactcg
    20041 ggggtgtaac tgctgcctcc accaatcgta gcgattttga ttccttttgc catgttctca
    20101 ctcctttact ttttacactt ttattatctt ttttgtaaac aaaacaaaca atagaaattc
    20161 gtttctttcc agtaacattt acaaaagtta gtgttttctt ctggattttt gtaaatatct
    20221 tgtcaaaaaa tgtctttatt aagctatata acataaatcc gtttacaaat cattttcaaa
    20281 aaagttataa tttacgtaaa aaaacaagaa gaaggtatct acatgttatt acgtgaaaaa
    20341 atcaaagaaa tctccttttc tccttcagaa aatgaagtga ttcagtttct cttaagcgat
    20401 tctccagcac ttggcgaaat gaccatccaa gagattgctg agcgatgtta tgtccacccg
    20461 tccacactca tccgtgtagc aaaaaaaatg ggttttgatg gctgggtcag ttttcgtaat
    20521 gcttttttag ctgaaacagc ctatcttgaa gggaattttc aagacgtaga tgcgaatctt
    20581 cctttttcca ccacagacgg tattatgacg atcgcaaata aaatggcttc tttagaacag
    20641 atgacgattc aagatacact ttctctcatt catcatgacc aactgcaaaa agccaaacaa
    20701 ctaatgcttc aagcaaaaaa aatcgtgatt ttcgccagta actcgaatac tttgatttct
    20761 caagatttca tgctgaaaat gaagcgcatc aaaatggatg tcacgattgt caccactttt
    20821 ggtgaatcgt tctatgaagc gtacaattgc acgagtgata tgtgcgggat tctgatttct
    20881 tatacgggtg aaaacaatgt gatccttcgt accgcagaaa tattgaaaca aaatcatgtg
    20941 ccaattttag ctttgaccag tatcggtgat tcaacgctat cttcaatgag tacagccgct
    21001 ttgcgactta cgacacgcga acgactgtat tcgaaaattg gtaattttac gatcaataca
    21061 tcgatttgtt atcttctaga cgtgttatat agttgtgttt ttgcagaaaa ttatcaacag
    21121 aatttagtcc atctaatttc tgtaggacaa aaatttgata aacgccctat cagctcagcc
    21181 atcatgaaag agcctttcaa ttttgaaaat aaagaagtgg actaaaaaga ataattaagc
    21241 ctagtccatt ctttcaccta accgttcagg tcagtgcggt gataagtgca tcgtctggcc
    21301 cgaccgtctc cccttgattt tccaaaatat ccaaataatc cgtgtaattg gtgacgatca
    21361 caggtgtttc taaactgtat ccttcctctt cgattttctt cttgtcgaag cggagcaaca
    21421 gatcgccttg tttcactctt tctccttgct gcaccaagct tttaaaatat tttccttcta
    21481 actgaacagt atccaatccg acatgaatca gcaattccag acctgcttca gaaaccattc
    21541 cgattgcatg tttcgtcggg aacagggtca tgactgttcc gtcaaaaggg gcacgtactt
    21601 ctcctttttc agggtagatc aagattcctc gacctaatgt tccttgtgca aaagctgcat
    21661 cctttgctgc attcagtgga gcgatttgtc cttgtatcgg tgtttttaca atttctttac
    21721 ctgatgtttt ctgtctatca tctgtttcat ctgcttcctc cttccaaaag aaaaaagtca
    21781 gcgcaaagga taccaccatc gcaatggcga tcccaatcaa tacattaatc acatctgtta
    21841 aattaccatc tggtctaata tagttgggaa ttgtaaagat gcctagtcca ccttgattcc
    21901 aagaagttaa attcattaac ccactatacg cgccccctaa cgcaccaccg acacaggtaa
    21961 aaataaatat ttttttacgt ggcaatgtaa acccataaat agctggctct gtaatcccca
    22021 caataccaga aacaatggct ggtattgcaa ttgctttgtc ttttggattt ctaagcttaa
    22081 agtagagtgc agtaagtgct gcagtttgtc caaaagacac tactgcaaca ggtgtcatga
    22141 tattttgcca gccattcaca gaaaattgca tcaatcccat tggtacgatt gcccaatgga
    22201 gaccaaacat cattagaatt tgccagaaga agcctacgat tgctccataa ataactggcg
    22261 aaagtgctaa aagtgtttca aatccagcca ttaaaatatc agttgcgaat gtgaatgctg
    22321 gtccaacgag taaaaaacct aagggcattg taataagaac ggtgaagaat ggaacgaaga
    22381 aattcgcgat catctcaggt actaactttt taattggttt ctgaatttgg ctagctaaca
    22441 acacaataaa gataatggga atgacagaag aagcgtagtt acgcgcaatc cagggaatgc
    22501 cgaatactgt ttgataaata ggtgcttcaa aaatagtgcc agaaaatatg gtcgtcagtg
    22561 gtgcttctgc tgcaccactc aaagcttcca gcgacaagct aggattcatt aatgctgccc
    22621 caagcaacat ccctacgaat tcatttactt taaatttacg ggcagctgtt aaaccgataa
    22681 taacaggcat aaattggaat acagaatcac cgatattatc aaacataaca aatgtgccag
    22741 aaccgcggtc aaatgcgcct aagaagacaa gtaatgctgt cagtccacga atcatcccac
    22801 cagcagctag aatagcaagg aatggctgga aacagcccga tattccatca ataagcatat
    22861 caaacggtct cattttttct gttgaaacag cctcatcaaa agtaaggttt cctgctctta
    22921 taacctcttc aaacacgact ggcacatgat tcccgatgac aacttggtac tgtcctcccg
    22981 ctcgcattac cgtcacaaca ccgtccatat ttttcaagat ctcatcattt gctttttctt
    23041 cgttttttaa tttgaaacga agccttgtga tacaatgtgt caaaccattg atattttcct
    23101 tcccgccgac attttctaca attttgctcg ctaattcttg atattttccc atttctattc
    23161 cctccaattt attagtggaa ggtttagcca actgaatgtc actaccctta tttcatgcgt
    23221 tcttgttaat ttatcctcct tttttattta tttacaacga ttctcatgca ctaaacgcgc
    23281 agtatggatc gttaaataca ttttttcgtc cgtggacatc tggtagttga atatctcggc
    23341 taaaaagtct gctattttct caacgcaacc aaacgtatcc ttatattttt tttgaataac
    23401 ttggtacaat tcctcagaaa cttctcccac ttctgatttt tctccatcta ggacgcgtgt
    23461 cagaaaaaaa cgtaggtgtg tgacgaatcg aaaaaaatgg actgaatctt catcaaatgt
    23521 aatcttatag taatagcgga cgatatttag aatatcctgt attgttgtag taaacaggta
    23581 ggtattgtct tgttggcttt gaatgccttg tgcattgact aaatgcaatg cgataaatcc
    23641 ggcttcatct tctgataact tgatgtggta tttatccgag attttcttca ctgttttttc
    23701 cccgatggca tactctttag gaaatagttg tttgatatca tacagcaata catttggtac
    23761 aaatattccc attttgtgac gttcgatagc cgtatacaaa tgatccgtca atgcaatata
    23821 cactgaatca ttcaactgga cttgtagagc ctcttttgta tattcaatga tttcacaaga
    23881 aatttctaag tactcaagag gaatcgcttg tgccaattct tggaactgtt ctgaaagtcc
    23941 gtcatttgtc aactgaaatt ttttgtcgat gcatgagtca aaaatcgggt cacccacttt
    24001 ctttttgaat gccaaacctt ttcccatcac gatcatttcc tgatttcttt catctctggt
    24061 tattacgacg ttgttattca gtactttctc aatcagcatg ttttactcct ttccatgata
    24121 tatagggctg tatccaaaac aaaaagaaaa acccaaaaag ccgtccatgg aaccatggga
    24181 gactcatcag gtttagcttg catcaggcaa tcactatcca agttgtatcg ttttctttat
    24241 attatcgaaa gcaagcgctt ttgtcaacgc tttcttagtg gctattaggc acttctgact
    24301 agctgtcgaa tcttttttaa atctggtgac tacgacacta ccgctcacag atgaagaatc
    24361 tgggaagctc tgttttattt tcactctagc gcttaggtca aaatgctact atgctactgt
    24421 cccaacttct aatatacggt gttccagtat cagcttatcg gtgataagcc aaatttatgc
    24481 aaaaatctgg aaagcctttt ttacaatttt tttcttatca cctaaagctg aacggagccc
    24541 tgtcacaacc actttttaca caccactgct ttgcagtttg tacatatctg catacaaacc
    24601 attttgcgct agaagtgttt catgattccc gcgttctact atatgacctt tgtctaaaac
    24661 taaaatcaat tctgcatccc gaatcgtgga cagccgatgg gcaatcgcaa tcgttgtccg
    24721 tccttgtcgc atttttgtca atccttcttg gatcaatcct tctgtttcag tatcgatatt
    24781 tgctgtcgct tcatccaata ctagtatttt aggatctgtc acgattgttc gcgcaaaagt
    24841 gatcagctgt cgttgtccgc tagaatagct ggcaccgtgt tcgatcactt ttgagtgata
    24901 tccttttggt aattcatcga tgaacttgtt tgcttgaaca aactcagctg catggcgaat
    24961 ctgttcgtct gtgatagttg gattcatcat ccggatattt cctgcgatat ctccataaaa
    25021 caagaaggca tcttgcaaga ccaatcccat tttcttacgt aactcttcca ttggatattc
    25081 tcgaatgtca tgatcatcaa tcaagatctg gccttcataa aattcgtaaa accgcatcaa
    25141 tacgttgatg atcgagcttt ttccgcttcc ggtgtgcccc actaatgcaa cggtctctcc
    25201 cggattagcg acaaaagaga tattgttcaa tacattgtgc tctccatcat aagaaaagct
    25261 tacattgcga aactcgatct tacctgaagt aatcgtttga tctgctccag gattttgttg
    25321 cggtgcatat tcttctgtat ccatgatttt caaaatacga ctaccggcaa ccattccatc
    25381 tgtaaaggtg ctcaaaaagt ccatcatttg agacattgga ttaaaaaatc cctggacata
    25441 ggtcgtaaat gcatagatca gtccaacttc tacgggagaa tgcagcgcat cgatcccaaa
    25501 caaagtcaac gccaacgcaa tcgccaatgc atatagaaaa ttgatgatcg gacttaacag
    25561 aatcgaattc gttttgatca tcgcatagcg cgtgtctaaa tactcgttgt tcacttgttc
    25621 aaattcttta gctaaacgag cttcttggcg aaactgctgg ataatacgca tgccggaaat
    25681 cgattcattc aatttcgtgt tcagctgact taatttttcc cgcatattcc ggtaaatacg
    25741 agaactgaat ttctggtaat accagatgac gattcctaaa atcggcaaga acaccaaagt
    25801 ccacaaggca atttctccat tgatttgaaa catggcaatg aaggaagaaa cgactgcaaa
    25861 gatcccagtc aataccatca aaaagacata ccaaaattca aatagcgttt ctgtgtcatt
    25921 tgtcactcga gaaacggtcg atcctgccgg tgtctgatca aaataacgca tacctagtgt
    25981 atgcagcttt tcaaataatc ttacccgaac atactgatag gtcttcaatg aagccatgga
    26041 atacaaatac cattggaaaa accaaatgat acttttgact atgactccaa acaagtacat
    26101 accagcaaaa aacaaaataa cttgtgtcgt agccgactgt ttagccagat gatcatccat
    26161 aaaagtttgg atgatccttg gcaataggac attgatcaca gacagagcaa acgcaaataa
    26221 gatagcaacg atgaacgttc ttctaaaagg tttggcgaac ttcatcagtc gtttgatgat
    26281 cttcttctgt tctttaagtg gaatcgattt tgtccattcc gattgataat tttcatccat
    26341 caggcctcgc ctccttctat cttcgcttct aattgctgtt tctcccacat tcgttcatac
    26401 cagccgccta atgcaagcag ctcatgatgg gttcctcgtt caatgatttt cccttgatcg
    26461 acaacaatga tttcttttgc atgcatcacg ctgcttaaac ggtgagctgc aatgatggtt
    26521 gttttctctt ttcttatttc ttttaaatta tgcaagatag cttcttctgt tttggcatct
    26581 actgcagaaa gtgcatcatc taagatcagc aattcaggat ttttgatcaa tgctcgagca
    26641 atcgaaatcc tttgtttctg tccgccagat aaagagactc cacgttctcc aactaaggta
    26701 tcataacctt ttggcaattc cttgatttca ttgttgataa atgctaaaga agccgcatgc
    26761 tctacttctt cttctgaaag ttttggatta gaaaaacgga tattatctcg aatggtcatc
    26821 gaaaacaaga agtgatcttg aggcacatag ccgattgatc ctaataaagc attcaatgaa
    26881 tattctttga tcgaacgtcc gccaaattgg atttttcctt gataatcatc gtattcacgc
    26941 atcagtaatt tgatgatcgt cgtttttccc gcaccagttt tcccaacaat acctaatgtt
    27001 tctccttctt ggatcacaaa tttgatttgt tctaatgctg gtgattcttc tttcggatag
    27061 ttaaaagagg aaatagctac attcagctcg ccttttgccg gggtctgcac tgcacccagt
    27121 tgttccacga tatgcgtctt ttcatgcaaa agttcattga cgcgatcata agaagcattt
    27181 cctctttcca atacgttgaa caatcgacca atcgcaaaca tcggccaaac aagcataccg
    27241 atatagctaa taaatgagac catctgcccg atcgagatcg tgccttccaa gacatacttt
    27301 ccacctaaaa taattgttgc aacataagac agaccgataa tcaaggtaat gaatggatca
    27361 aataaggagt ctaaaaaatt gactcgtttg tttttaataa ttgcatcttc aatcttttct
    27421 tcaaaatccg cgagatcttc tttttcttgt ccaaatgttt taatgacttt gattccagta
    27481 atactttctt gtgtcttatc attgatacta gaaaaagctt cttgcgaatc acgaaaagca
    27541 tcgtggagtt ttgaccccag cactcgtgaa gtaacggcta gtaaaggcag aggaatcagc
    27601 gcaatcagtg ttaagcgcca atcaataaac aaaatcatcg caatgatcgt tgttccgccc
    27661 gtgatcactg aatctgcaaa ggtcaagata ccagctcctg ctacattttg gattgcattc
    27721 aaatcattcg ttgcatgagc catcaaatct ccagtccgat atttttggta aaagagattg
    27781 tccatttctg taaaatgttt gaatagctgc tgccgaagtt ctttctccaa ccgagctgca
    27841 cttccccaaa tattcgtccg ccagatataa cgaaaaagat attgtaagag agctgcaagg
    27901 atcaataata agatccacca ggaaatgatc tttattgata aatttttatc ggcaatctca
    27961 tcaatgatga tcccaatcac ctttggagga actaactgta ccaaggccac tgcgaatagg
    28021 gagaaaatcc caattaaata atgtttcttt tcctgtcgaa aaaaccaacc taattttcta
    28081 aaaatcgaca ttttactcct cctttgtctt tatcaagagc ataggattgt ctgtgcttgt
    28141 cttttttgct gaatagtctg aatagtctaa caaattacga aataaaaaac ggactgaaaa
    28201 aatcagcccg caaaaagtct ttgggtcaga acctattttt tcccttggtt cttcatttgc
    28261 tgcatcatct ggcgaatttt cttctcagat ggtttttgcc ccattgacat catcattgat
    28321 cgcaacatgt cctcattaac gggaggattt ttttctaaat agtctttcat atatttacgc
    28381 gctaggaaaa agccacctac tgcgccgatt agcaaagcaa taatagcaat aagtacgaca
    28441 atacctgttg ataccatata aatctctcct ttctatttcc gaattgctct caatgattta
    28501 ttttactaaa gtttattgaa aaaagaaagg cgtattcttt agtttcatgc aaaaaatagt
    28561 ggttttttta gagaagaaca aaatttcttt cattaaccag caagacacgc cacgaaaaaa
    28621 aattaggcat accgaatatt tttctttaat tttttctttc tgaggtgtag aatgtaatcg
    28681 tagataaata ggaaaggtgg ttttttcttg aataatcaat ccaacaaaca atttcataag
    28741 catcgactta ttgaacatac aaatggttta tctttagctg aagtaaatgg aacagtggat
    28801 gtacccaagg gaaaaagttt ttttaaaaca cttcttgctt actctggacc aggtgcatta
    28861 gtagcagtgg gttatatgga tccaggtaac tggtccactt cgatcactgg tggacaaaat
    28921 ttccaatatt tattgatgtc agttattttg atgtctagtc tgattgctat gcttttacaa
    28981 tatatggcag caaaattagg aatcgtgacc caaatggatt tggcccaagc aatccgagca
    29041 cggacaagta agacattggg tgtcatttta tggatcttga ctgaattagc cattatggca
    29101 acagacatcg cagaagtcat tggtgcagca atcgccctct acctgctttt tgatattcca
    29161 ttagtgattg ccgtatttat cactgtgtta gatgttttcg tcctcttatt gctgacaaaa
    29221 gtcgggtttc gtaaaatcga agctttggtt gtctgcttga ttttcgtcat tttattcgtt
    29281 ttcgtctatc aagttggctt gtctaaccct gattggcaca gtttgtttgc cggctttatc
    29341 cctaacagtc atacattctc agactcaccg gtcatcggcg gtgaaactcc gctgactgga
    29401 gctttaggga ttattggtgc aacagtcatg ccgcataact tatatcttca ttcagcagtt
    29461 tctcaaactc ggaagatcga tcaccaagat gaagaagatg tagcacaagc ggtacgcttt
    29521 tctacttggg attctaatat ccagttgtca atggcttttc tagtcaatgc actgctattg
    29581 atcatgggtg tcgctgtatt caaaaccggt tctgtccaag atccttcctt ttttggtctg
    29641 tatgaagctt tatctgatcc atcagctatg agcaatggta tcttgattgc tgtcgcaaaa
    29701 tctggtatct tgtccacttt atttgctgta gcacttcttg cttctggcca gaactctact
    29761 attactggta cgttgaccgg tcaagtcatc atggaaggct tcatccatat gcgtatcccg
    29821 atttggctaa gaagactagt cacacgattg ctttctgtga ttcctgtatt gatttgtgtt
    29881 ttgtttacaa gtacaaaagg aacgatcgaa gaacataccg ctttgaataa cttgatgaat
    29941 gaatcccaag tttttctggc ttttgcattg ccattctcga tgattccttt actgatgatg
    30001 accaatagcc aagcggagat ggggagccga ttcaaaaatt catttgttgt aaaactactt
    30061 ggctggttct ctgtgattag tctgacttac ttgaacttac gaggattacc aggtcaaatc
    30121 gaaagtttct tcggcgagca agccacctca gctcaactag cattggctga tcaaatcgct
    30181 tatgtaatca ttgttcttgt ccttctcctg ttggcatgga cgattggtga tttatataaa
    30241 ggaaacaaaa aatatgaaca gcgtttgcaa gaaacgatag aagaaatcga atagaaactg
    30301 attcttttac acaaaggagg ttgtgacaga agtgggcagc ttcaagaaat aagaaggaat
    30361 ttaagaaaat tgcttttcaa atttttgtga aatttcggct tatttttcga agaagttact
    30421 tctggaacac cgtgtatcag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    30481 gccaaacaaa aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa
    30541 gcttttgaac accgtttatt cggaaaaagg aactgcgaca agacttttgt cacagttccc
    30601 tttttattta gaagataaag cttttttcag attcaattta ctgaaaacta gcatgcttgt
    30661 tactacgatg aattgtgtta atggcacgcc tgcgccagtg tagacactta aaaaataatc
    30721 ctttttttct ggcgcataag aacaaaacca cgagacaccg aaaagaaaaa ccaattgtgt
    30781 cagtattaat ccggtgacta caatcaaaga ggaactataa taggcacgat cagcttttgc
    30841 aaatacggtt cctagtatca taagtaatat ccctgctatt gaagcaatca gcacgggaat
    30901 ggccagaact tggtaaattt ttatcatgac attcgcgact tttgctgttt ttgtcaactg
    30961 gaaatcaggt gttctcggat cactgctatc gatccaatta tccattagtg gaagattcaa
    31021 taattcttgg gcctgttcga cttgttcttg cgtcccgaaa ctataattcc ctccttgatg
    31081 gaaatctttg tatcgccatg cataggcata tcctgatttc agaaaagcaa atacgattgg
    31141 cagatcattc atcgtctttc catcccctga tcccatcaga tagaattctt ttttcttttt
    31201 gatagttccc ttttgatagg cttcttctaa ttcttgattg acttttttcc aaaaatcatt
    31261 ggtttctttt gcattgtttt ggtaataacc agattcgctt accacatctc gcaatgccca
    31321 aaaaatgata tctcctggta attcttttac ttttccgcct tgggtccacg catgagtcgt
    31381 atacaattcg tcaatacgag tagaaacagt atgaaatgta ggagatgcgt ctaatgcttt
    31441 atttaatgca gcatgcgaga cccatatctc actgttttct ttatttaagt tcgtttcgtc
    31501 atctattcgg atcagctgtt tcatcaaacg tccaaattca ccactcgaac ggtcattgat
    31561 ggtggcaata ccatatgttg tctcgttttt atattcgatc cactgataga cgatggagaa
    31621 accgactaga gggagtaaaa acaatgaaag tttcagccat tcttgtttcg aaaggagaaa
    31681 tggtcgttct tttaacttct ttgcttcttt gaaagaaaag tcttgtccta gcaagatctg
    31741 aacgaaactg atgcctaacg cgactacagt aaaaggaaga agccagatgg aatcttcgcg
    31801 aatgaaccaa aagaaaggaa aagaaacagc taaccctaaa aaccacggca gcaattttct
    31861 gcgggaacta tatttacggt aataaatagc tagtaaacaa gcgataatga tctcaacagc
    31921 cgggatcacg agagcattac gatacgttcg agtggaatac tcatgtgtaa aactaatcgg
    31981 agaaaacaaa aaataaaaat agatacttaa aagaagatac ttgttcttca cgatcggttt
    32041 aaacgctagt gcaaccaata tgctggcaag aatactgaac atgccgaaca aaactgcata
    32101 agaaagatgc agtttatgtg caagtactaa aaacaagctg taggagatcc cttttgaaag
    32161 tgtcgtagtc gtgtactccc ctagccattt accatccaaa atattttgag catagtgaat
    32221 cagcagctga tcatcatagg ttgccgacat atctgttcca tagattgctt tactatataa
    32281 atacaaccga ataattgtaa ataatacggc tatgccaaga ataaagtatg tatgtttctt
    32341 ttttagttgg ttgatcaagg gttttaaagt gttcagcatc ttttcgtcct ttcaatcacg
    32401 ttttgctact tgcttgtgaa tcttttcgtt ttttactttt ctatcagtat accctgtttg
    32461 tctgaattta aaaagagggg gcgaaaggag catggcgaaa acgactgcaa aaagcactcc
    32521 tttgtttctt aaacaaaaaa ggacaaggca gactgcccaa tccttttttg ttgctgtcag
    32581 atagaaaact taaagtgtga cttcttctgc taataactca ggaaacactt cgggatcgaa
    32641 tgcttgcgct ttgaacatgg caatttcttt tccatatggt gcgtaagctt tctttttatc
    32701 tttaccgaca tatggagttt ctaagatttt cgaaatgttg tcaaacattg gatcatggac
    32761 gattttgttc aatgtgtcaa atccaattgt tccaaaaccg atattggcat gccgatcttt
    32821 atgagaacct tgtggatttt tagaatcgtt gatatggagt actttaagcc gatctaatcc
    32881 gatgacctta tcaaactctt ccatcacacc tgagaaatct tcttttatat tatatccggc
    32941 atcattcgta tgacatgtat caaaagtaac ggataatttt tcattcaatt ttactccatc
    33001 gatgatttgc gctagttcct caaatgttcg accgatttcc gtacctttcc ctgccatcgt
    33061 ctccaaagcg atctgtgccg tctgttcttt tgtcaaaact tcattcaaac cctcaataat
    33121 tctagcaatc ccaccttctg cccctgctcc tacatgagcg cctgggtgaa gggtgatttg
    33181 agtagcacct aaagcttccg cacgaacaat ctcttctctc agaaattgta cagcaaatgg
    33241 aaacatttct ggtttgatcg tatttcccaa attaatgata tagggcgcat gaacgacgat
    33301 atttgttagt ccgttttctg ccataaatgc ttttccggca tcaatattca tctcttcgat
    33361 agcttttctt ctagtatttt gaggagcgcc tgtatagatc ataaaggtcg aagatccgta
    33421 actggctgct tcttctgccg cgcctaacag cattttcttt ccgctcatgc tgacgtgtga
    33481 gcctaataac atggatggat tcctccttga aaggcaattg tttcgaacaa ttcctatata
    33541 ttggtaagat gatgtacaaa gaaaatggtt tgtttttctt tgccctgttc tccatagtaa
    33601 ctgaaacttt ctctaaagaa aagcttaaga actctttcat aaaaaagatc atttcctcac
    33661 gttttatctc attcgaaaaa gaaattcctg tcaaaaacag ccttgtctgt ttttttgtct
    33721 tattatttgc tataattagc gaaataaaac cattagttga ggtgaccctt ttgccacgca
    33781 aaaatactcg aaaaaaacag aatagaagaa aaaaagaaaa atggtttgtt cctaaggtta
    33841 tttttagagt gtttcaatca ttgactgttt ttattactgt tcttcttttg ttgttcgcag
    33901 ctctaggtat cggaataggt gctggctact ttgcctatct tgtcgaagac acacaattgc
    33961 cgactaaaaa agaactgcag acagaactag gaaacattac ggaaacgtca aagattgttt
    34021 atgcagataa tacagagata tcaaaaatcc agacagatct gatgcggacg actatttcat
    34081 ctgataagat ttcgccttta ttgaaaaccg cgattatcag tacagaagat gaatacttcg
    34141 ataaacacca aggttatgtc cctaaagctg ttttacgagc attagtttca gaagctactg
    34201 gcatcggctc gtctggagga tccactttga cacagcaatt agtcaaacag cagatcctaa
    34261 ccgatgaaac aacattcaaa cgtaaagcaa acgagatcgt tctttctgca caagtagaaa
    34321 aatacttttc aaaagatgag atcattgcta cgtatttgaa tgtctcgcca tttggtcgga
    34381 ataataaggg gcaaaatatt gctggagtcc aggaagctgc aagaggaatc tttggtgtag
    34441 atgcaaaaga tgtgactctg ccacaagctg cttatatcgc tggtttgcct caaagcccta
    34501 tcacgtatag tccctacacg aatacaggcg cgctaaaaga agatctttct gccggtcttg
    34561 ccagaaaaga ttttgtttta ttcagtatgt accgtgaagg acaaattacg aaagaacaat
    34621 acgaagaagc gaaagcctat gatcttacaa aagactttct cccacaacaa atcgcggaac
    34681 agactgacag agaattcttg tattataccg tgatgaacga agcaacaagg attattgcac
    34741 aacaattggc agaaaaagat aatgctgata tgtcagattc atcggtcagt gaagcttatt
    34801 atcaaaaagc acagcaaacg atccaaaaca aaggatatac gatccattcg acgatcgata
    34861 aagatatcta tgctgcattg caaaccggcg tagaaaatta cggctatatg ttagatgatg
    34921 gtgctggtag ccaagtggag actggaaatg tattgatgga caataggact ggacgtatct
    34981 atggattcgt gggtggccgg aattacagcc agaatcaaaa taaccacgcc tttgatacag
    35041 aacgtcaagc tggttcatcc atcaaaccgg ttcttgttta cggtcctgca atcgatatgg
    35101 gacttgtcgg atcagaatcc cgtgtatcag attatgcaac gacttggcaa gaaggagaaa
    35161 atgctggcga gaaaatcgtc aatgcgacca atgaagggtc gaacaccttc caaaccgtcc
    35221 gtgagtcact ggaatggtcc aacaatatcc cagcttatca cttataccaa gacgtattga
    35281 attcgggtgg atcgaaacaa tatgcttacg agcactactt ggcgaagatg aactacccag
    35341 caaatgataa ttggggcgtt gaatctgctc ctttagggac agtagatgta acgacgcttc
    35401 aacagacaaa tggattccag gccttagcta atggtggcgt ctacgaagaa ggctatatca
    35461 ttgattccat cacagataat gcaggtaatg tgatttataa acatgaaagt aattctgtcc
    35521 gtatttattc cgaagcaacg gcctcgatca tgaatgacat gatgcgcagt gtgatcaatg
    35581 aaaaaatcac tacaccattc aaagatgtca tttcaagctt aaacggaaat ctaggaaaag
    35641 cagattgggt cggaaaaact ggttctacca acgaatacag agattcatgg ctgatcgtct
    35701 ctactccgtc tatcactatc agtagctggg caggacacga tgacaatact ggaatggaca
    35761 gccaagcaag gatacgttcg gctaattatc ttgccaatct aatcaatcaa gcttaccaag
    35821 caaaccctga gattttcggc actgatgaga aatttgactt atcatctgat gtgatgaaga
    35881 aaaaagtatc tgggttcaca ggacaaacag ctggaaaagt tactgtcaat aaaaaatcta
    35941 tccaaacgcc aggaaaaaca gtggaatcac tttgggcaaa aaatggtccg aagaaaagcg
    36001 cctttaaatt cggcgttggc ggtactgatg aaaactatac agattactgg aacaccgctt
    36061 cagcttatgc tagagcgaat ccagagacca aagaatcaga cgataaagat gattgatcta
    36121 atagattaat cagaattaaa gggagctgcg acaaaaatct tgtcacagct ccctttttcc
    36181 gaataaatgg tgttcaaaag cttgcatcta cgatattaag acaaatttat gtaaaaatgt
    36241 gaaaagccat tttcacaaaa tttttctata taactttact aacgaaactg ttttaatttc
    36301 ttttcttgtc tttggaataa ttcggttagt ttttctgcaa attgcccttt tactgattct
    36361 ttcaagtgta catgtcggtt agaaaattct tgagcagctt cttctgcagt catttggtgg
    36421 ttttgatctg tcaatcgttt ttctgcccaa atttgctgag atatccccat accaactgaa
    36481 cgtacatttg atcgatgtag atttttctct aattctttta gtaattgcat taaaaattca
    36541 ttcataagat gatttctcca tagttatttg tctgattttt tgacgatagg aacgttctag
    36601 ttcgtcttgc tgctgataga gttttcgtcg ttctacttgt agggcttgtt gataatgctc
    36661 ctgataattc gaataacttc gtcttgcttg aaacagaggc tcattatcta aagctaagtt
    36721 ctttaataca tgatgcagtc gctgctcctc ttgctccatc aatcgtaatc cttgattctt
    36781 aaagtggatc aattcatctt cttgagcttc taattgtctt cgttcacttc gataatctct
    36841 ttccaattct tctagtttca tctacttctc ctttaattga attgctgtgc taattctcga
    36901 tctcgtgtca cgatttcatt gattttgtct tggatctcct tcaccaatgt atcaaaggcc
    36961 tcccccatac gtcttgcctt attgattcgc agcagataga cttctttcgc agaatcgact
    37021 aagttctctt ttgtggcgac aacactagct aatgcttcta tgatttcctg ttcagtcaac
    37081 gaaggggcga caacacgtgc ttgccgcaag ccttcttgcc atgccatttt ttctctagct
    37141 caaaaattcc tctttgattg atgcggataa cttcttccat agcgcttttc atttttgctg
    37201 ataacttttc cactactaac ctagcttgtg tgtcatctaa gtaaatttgt tcattaactg
    37261 tcagccggcc gccacttaat cgtagttttt tacgtatgtt gttcagttct tgcaactgat
    37321 aggaaaacaa ctgggtcgct tggttttctt tctgttgttt tcgagcttta acattatttt
    37381 cattttcagg aataatcagc tttccatttt catcaaactt ccaagaggtt aaactgtggg
    37441 aagcaagcag attcaagaat gctgtttcca tactgatccg aacttcagca cctgtgccat
    37501 taccaccaaa attaccgata aaatcggcgt gatttcggta attggtgagc ataccgggat
    37561 tttcttccac ccattttttt gcttctggtg ataagagatc atacgggtcc ggcccgttta
    37621 agccgacatt tttccattgt ttctctgctg ctacatataa tgctaagaat ccacctaatg
    37681 aatggccggt agtagaaata gcagcttctc catattcctt ttttacttca ttagcaaatg
    37741 caatcgctga attgatttgc cccgctgttt tcaatccact aacggataaa atcttgtttg
    37801 ttgttgcttt ttcagaaaaa cttgctcctg gtctattccc atagagattc atgatactcc
    37861 ttgcatccgt ttccaaatct ttccaatcac ttaagttcgt tcctgcaaaa gcaatcacta
    37921 cttgagaaag atccacttta ccgttcttga ttggcgctac acccatcgcc tgcatgccgt
    37981 ccaatgagtc tttgattttt aggattttaa aattataact atcaattttt ttaatagaat
    38041 tttctttata agtatgatca tagtctctgt gtttaggatc tagccagtaa acagcctcac
    38101 taataacact gtattgtttg tctgccatca ttaaatatat tcctccttat tattagaaaa
    38161 aattactctg attttattta ctgtaatccc ctctttttgt atacttttat tttctacgcc
    38221 ataatctcct aattcatttc gttctttcca aaatccaaac gtaaaataaa cagactcatc
    38281 gtgattattt atcattgtca caaacatacg atatgctccc gtcatttcat ttcgagcaga
    38341 tttttcaatc ttcacttcct tgatatccgc aaaagtattt ttcaaaactt tgactgactg
    38401 tttttctact gcgagtaaat ctttctgtct ttgtgcttct cttttgtcca tatagacttt
    38461 ccctccgatt cctaatatga cgattaataa aatggctaag ctgagttttc ttattttccg
    38521 agacaagaac gtgcctcctt tctttttatg taagtattga taccttcatt atttaagtac
    38581 gcgaaagttt gtaggaattt ttttattttt gaaatttttc tccaatttgc tatggacgat
    38641 tggcgctgca gccatcgcct gcatgccatc caatgagtct ttgattttta ggatttggaa
    38701 ttttgtttct ccaaagtttt taatggcgcc ttctttgtaa cttttactat attgaggatg
    38761 ttttggatct agccaataaa catgttcact tatatatcta tactcttcgt cttttactgt
    38821 cacacttcct ccgcctctcc attagaataa ataactctta ttttaggagt agtaacccct
    38881 tcgttttgta tttcttcatt ttctacacca taactgctaa tttcattcat ttgttttgaa
    38941 aaaaaataat caaaataaac agatttccct tcattatttg tcattgtcac aagtgcacca
    39001 taacatccag taacagggtt ttttttaaat tcttctacct tcacttccct gatatctgca
    39061 aaggtatttt tcagaacttt gacggattgt ttttctactg cgagtaaatc tttctgtctt
    39121 tgtgcttctc ttttgtccat gtaaaatttt cctccgattc ctatagcaag aatggtcaaa
    39181 atgatgacta tggtctttcg tcttttctta ggcaaataga agtctccttt cgggttatgt
    39241 atgatggatt gtttctcctt taagataagt acgcaatcga gcctggattt ttttggattt
    39301 tttagagtta cttttctact ttctttttta tcacgttttc attttaaagt aattttgata
    39361 gtagtgaaca aaatgtagca tctacgcaca gattgtgtcg tatacgatgg acgtaaggtt
    39421 tgtttccgcc attttctctt tttttaaagt cacaaaaaaa aggggttatg acaaaatttt
    39481 gtcataaccc cttttctcga ataaacgttg ttccagaagt tggccacttt tggaacaacc
    39541 tctaaatgat tgatctttta ttatttagtt gatccttttt cttcgattcc ataaggcaag
    39601 ataatcgttt tttcttcgac ttcttcttta ttcatcattt tcgtcaatag gcgcatggat
    39661 accgctccta aatcgtataa tggttgtgtg atacttgtca aacgaggacg agctacttct
    39721 gtcagtaaag aattgttgct tgtgatgatt tcgaattctt ctggtacttt tacgccacgg
    39781 tctaataaac catccaagat accaatggct aattcatcgt ctgaaacata gacagctgtt
    39841 gcaccgctat tatggatccg gtcaactaga gacagaccat ctttaaagtt gtattgtgcc
    39901 tcaaagatta atccttcgct atatggtaga ttgttttcgc ttaatgcttc tttatagcct
    39961 tttagacggt tttgaccatt gataggatca atcagcgcac cgctgacaaa agcaattttt
    40021 ttattgccgc tttttgctaa tttatttact gcatcttttg ttgctgatac ataatcaatg
    40081 tttacgctac ctacttgttc atctggatca atggaaccag ctagtacaac aggtgttttt
    40141 gaacgtgaaa attctccacg gatttcgtct gtgatatggt gacccataaa gatcacgccg
    40201 tccacttgct tagccaataa attattcaat acattgactt ctttttgatc gttgccatct
    40261 gagtttgcta agatgatatt gtatttgtac attgttgcta catcgtcgat accacgtgct
    40321 aaagaggcaa agaacatatt gctgacatca ggaatgatta ccccaacagt tgttgttttc
    40381 ttacttgcca agccacgtgc gactgcattt ggacgataat ccaagcgatc gataacttcc
    40441 aagacttttt ttcttgttgc tggtttgacg tttggattgc catttacgac acgtgaaaca
    40501 gtagccatcg aaacatttgc ctctctcgct acatcataga tagtgattgt ttgcttttcc
    40561 atttgtgttc tcctattcat attttatatg ttttctgaaa atacgtctta catttctaaa
    40621 ataggatatc aaataatctc aacaattgca accgttttat ctctgttttc atgaaaattt
    40681 ttactttttt cagttttaag aacgttggtc ttttaatcaa tcagtggtac aattattgaa
    40741 tacaaaatta atttagaaag taggttgcca aatgaataac gtaaaaatca atgaactacg
    40801 tcaatggatg gcacaacacc aagtagaatt ggcttatatc agcgaccctg gccacatcgc
    40861 ttacttttcc ggatatgaaa gtgatcctca cgagcgtgta ctagctcttt ttatccctct
    40921 tgaacaagat cctttccttt tcactcctgc tctggaagtt gaagatgctc aaaacagcag
    40981 ctgggcgtac gatgtacgtg gctacttaga cagcgaaaac ccttgggaga tcatcggaaa
    41041 agagcttcgc gcacgctatg ggcaacctgg taatgtaggg attgaaaaga atgctctgac
    41101 tgtcgatcgc ttcgatgcta tctctgatat cttgacaggt gcagattctt acactgatct
    41161 aacttctatt atccagcgtc tgcaactgat caagacggta gaagaaaaag acaaactgat
    41221 cgaagctggt aaatgggccg atgttgcctt tgaaattggc ttcaaaacag taaaagaagg
    41281 cgtgactgaa caagcaatta tcgcagaaat cgaataccaa ttaaaaaaac aaggtgtttc
    41341 acaaatgtct ttcgacacct tagtccttgc aggagcaaat gctgcaagcc ctcatggtac
    41401 accaggaagc acgaaggttt caccaaatga actagtgtta ttcgatcttg gcgttgtttg
    41461 gaatggctat tgcagtgatg ctactcgtac agttgcttat caaaaaccaa cagaattcca
    41521 agaaaaaatc tacaacatca ctcttgaagc acagcttgcc gcacaagagg cagttcgccc
    41581 aggtgtgaca gcaggtgaac ttgatcaaat cgcacgaaac gtaatcaaca gctatggcta
    41641 cggcgaatat ttcaatcacc gtttaggtca cggaattggg acaaccgtcc acgaattccc
    41701 ttctcttgta gaaggaaacg acttagtgat cgaagaaggg atgtgcttct ctcttgaacc
    41761 aggaatctat attcctgaaa aagtaggggt acgtatcgaa gattgtgtgt atgtgacaag
    41821 tgatggttgt gtgccattca cgactacacc aaaagaatta cttgttttag agtaatgaaa
    41881 ataaaaaagg tgggagctgg tccgctctca cctttttatt ctagaaatga gtattcttag
    41941 tgattggtag tcgaatctgt cattgtatct ttcatttctt ttttagcttc ttttgcatct
    42001 tcttttgcca ttttcaacat ttcttttgtg tcatctgcaa ctggttttgc ttctgacatt
    42061 ccttcattga ccgtctctcc tgctttatct gcagcatctt ttacatcgat catgatatct
    42121 tctgaagtat ctttagcaat actacctaat tcgtctgctg gttttttcag atcttctgct
    42181 gtttttttga aacgattaga taaatcagct gattgttctt tgaggtttaa caagatatca
    42241 tctgattcac ttcctgcttc ctctgctgat tcacttacct tttgcttaac attacctgct
    42301 agatcccctg cttgttgaga aagatcttgc gctttttctt ttgcaatgcc tgttaactca
    42361 gctgattttt cttttgccat atctgtgtaa tctgaagcca tgtccatcaa atcatctgct
    42421 tgagcagcca attcatcccg aagttcttta ccagatttgg gtgctaacaa taatgcggcg
    42481 attgctgcgg ctgtacctcc aactactgca cctaataaaa atccaccttt tttagccatt
    42541 cttattttcc tcctgtcgtt tcagtatgtt ttttgttttt aaaaaatttc atcgctgttc
    42601 caccgacttt tccggcaaca gtcgctttag ctgtcgtttt tccgattccg ccgactttcg
    42661 tcacgatgtt tttactagaa tgattaagtt ccgatacact ttcacttaaa tcagcaactg
    42721 ctgtgaacaa tggatcgatc gttgcgactt ttccattgat atccaagagc agctcattac
    42781 ttttgaccag caagccttct acttgtctag ataaaagatc tacgtctttt gtgacgatct
    42841 caattgtcgt atttgcttct gcgacagtct ggtctacttt ttctaaagtt ttggaaactt
    42901 gcaacaatac gcgaatgatg aaaacgacaa gtatcgcaaa ggcgattgct gcgatcaatc
    42961 ctgcaatttc tcctcctgac atgatcaaac tcccttcttg tctctctttt ctataaggat
    43021 agcattttcg tgttttttaa acaaataaaa acgagcttca actacttttt attcaattag
    43081 cagctgaagc tcgttagaaa aaaatgatta ttttttcttt tcttctttga tttctgttat
    43141 tccttttgga ttatgatcga tcttgcttcc ttctgcttct aatgttttac ctaaagacat
    43201 gatgtattca cgcaaaggtg cccctacttc tggatgtgtc agaccatatt caatacttgt
    43261 tttcataaaa ccaaatttgt caccaacgtc ataacgttta cctgtgaatt tacgggcaaa
    43321 tacgcgttgt gttttattca acgtatcgat tgcgtcagtt agttggatct catttccagc
    43381 acctggttgt tgttctgata atacatcaaa gatttctgga gtcaataagt aacgcccaat
    43441 aatagctaaa tcacttggtg ctttgcttgg atctggtttt tcaacaaagt ttttgacatt
    43501 gtataaccca tcttccagta cttcccctgg gttgataatt ccgtattttg aagtttcatc
    43561 gtgaggaact tgcataacgg caatcgtaga agcgtgtgta cgttcatagt catcgatcaa
    43621 ttgctgtgtc aatggtactt cgtcttccat gatatcatcg cctagcataa cgacaaacgg
    43681 ttcatttcca ataaaggctc ttgcttgaag aacggcgtgt cctaacccct ttggatgaga
    43741 ttgacggatg aaatgcaagt tgacatctgt cgtttcttct actaatttca ataattcgat
    43801 ttttcctttt tcacttaggt ttgtttctaa ttcgatattt gaatcaaagt gatcttcgat
    43861 tggtcgttta gcttttcctg taacgatcaa gatgtcttcg attcctgatt tcaacgcttc
    43921 ttcaacgatg aattggatcg ttggtttgtc cacgattggc aacatttcct ttgccatcgc
    43981 ctttgttgct ggtaaaaatc ttgttcctaa tcctgctgct ggaataactg ctttttttac
    44041 tttcatatac attcgctcct tcaaaaaatt gttaatgaga attcattttc ttttttccca
    44101 tcacgtaaca tgatttcttt tgcggctgtt ttgatttctt tttgttcata cagcacttca
    44161 tagattgttt gagtgatggg catttcgatt tccatcattt gggctagttc catcgctgct
    44221 tgagtagtat tcacaccttc aacgaccatt cccatattgg ctagcacctc ttctagaggc
    44281 tgtccttgtc ctaacaagtt gcctgcgcgc cagtttctgg aatgaacact tgtgcaagta
    44341 acgactagat cgcctactcc gcttaagcca ataaaggtca gcgggttggc acccattgct
    44401 acacctaaac ggctgatctc agctaatccg cgcgtcatga ttgccgcttt tgcattatcg
    44461 ccaaacccta agccgtgaat agcaccagct ccaatggcaa taatattttt caaagctgcc
    44521 cccatctcta caccaaccac atcagggtta gtatagattc gaaaataatg gttcatgaac
    44581 agctcttgta catattttgc tgcttttgca tctgtgcttg ccgctgtgat cgtcgtaatg
    44641 tcatgctttg ctacttcttc cgcatggctt ggcccagaaa gaacagcaac agcttttctt
    44701 ttttctgtcg ggatttcctc taacaagatt tcagagattc ttttatgcgt tccctgttct
    44761 aatcccttac ttgcgtggat gatcactggc ttcgtattca atttggcaac tagttctttt
    44821 gcaacagaac gtatcgcttt cgtcgggata acgaatagta ccgcatctgc atcttttact
    44881 gcatcttcca tatcggtgaa tgctcggatg gaatcgggta gatgatgatc tggtaaataa
    44941 cgttgattgg tgtgttgggt gttgatttct tctacttgtg aagcacgatg tccccaaatc
    45001 cgaacgtcat gtccgttttc agcgaggact tgtgctaaag cagtccccca agagcctggt
    45061 cctaaaaccg ctactctata attcatttta ccactcctga aatttttttc atttttaacg
    45121 ctctaaaaat caaataattt gttgattttt tcctaatgag cggtcataat ccggtaaatt
    45181 tccttttttt ctacgccaaa taaacagaat caatgtgccg acaagtaaaa cagcagacaa
    45241 taattgagaa atgcggatca cacctaaaaa gtaaaggctg tctgtgcgta atccttcgat
    45301 aaagaaacgt ccgaaactat accacattag ataacataaa aggacttctc cttttttcaa
    45361 aagatgcggt ttcttccgca aaaccaaaag cagaataaat ccaatcaaac tccaaagtga
    45421 ttcataaaga aaggttggtt gtcgatagat cccttcgata tacatgttat caatgatgaa
    45481 ttgcggtaaa tgcagacttt ctaagaaact tcgtgtcgtg tctggaccat acgcttcgtg
    45541 gttcatgaaa tttccccatc gtccgattgc ctgcgccagt aatacacttg gtgcagcgat
    45601 atctaaaaag gtccacaaag aaataaaatg atatttggta aaaaagtaca gtgtgattgc
    45661 acctgcaatc aatcccccgt aaatcgctaa tcctcctgaa cggatattga atatttggat
    45721 tggatcagct atatattggg gaagatcgaa taaaacataa tacaaacggg caccaataat
    45781 ggaaatcggc aatccccaca acataaaatc tgtcacgtca tctgctttta atcctacccg
    45841 aacagcttct ctgctgctga gccacatggc aataatgatt cctgatacga taatcaatgc
    45901 ataccagtag ataggaattc ctacaagctc aaaagcaact cgattgattt gtgctaacat
    45961 attgtcactc cttgctagtc attgcctgag ttttcttcaa tcaagcgact taatctttct
    46021 tcaaatgttt tcgttgcgtc aaagcccatt gtacgcgcgc ggaaattcat cgcggctact
    46081 tcaatgatga ttgcaacgtt tcgtcctgtt ttgacaggga tttttatttg tggaatgccc
    46141 acttccgcga tttcaacggt cgtgtcttca gaacccaaac ggtcgtattt cttatctttc
    46201 gcccatgctt ctagataaac agctaactgg acttgcatag agcctcgtac cgcactcgca
    46261 ccgaaaagat tcattacatc tatgatcccg atccctcgaa tctcaatcag atgttccagg
    46321 attttaggag gttctccgat taccgtcagt tcatcttgct gatagacatc tactcgatcg
    46381 tctgcgatca atcgatgtcc gcgcttaatc agttccaacg ccgtttcact tttccctatc
    46441 ccactgtccc cttgaattag tacacctaat ccgtagacat cgactaacac accgtggaca
    46501 cttgttcgag gggctaagcg cccatctaaa taactagata attcacctag cagccgagac
    46561 gttgagatag gcgaacgcag cacagctatc ccattttcat tggctgcttg gatcatttct
    46621 tccggagccg gcaatcctct tgaaataata aatgcgggca tatcttcact acataatctt
    46681 ctcatgatga tcaaacgctc ttctgggatc atccgttccg caaaactgat ttctttactg
    46741 ccaaatagct gtaaacgatt atgagaataa taattaaaat aaccggtcaa ctctagtcct
    46801 gggcgtgaga tgtccccagt cacgatgacg cggtctaaac ttttctcgtc gcctgtgacg
    46861 atttccaaag ataatttttt tactaattgg ctaactttta caacttctga cattcatgct
    46921 acctcatttc tattagagtg ttctctacca ttttatcatt gtctagccaa aagtactacc
    46981 aattaaaaaa aacgttttta tttaattacc aatgtaatag aaaacgcatt cttaattata
    47041 tatgatttgt caaattaagc tagaataaaa tgcttcttgt acatagctca aaaatatttc
    47101 gattggtgtt ctgtaattca atgattttct tggaatataa ttacgttgat tgctgacact
    47161 ggaaataaat gtctgattca cttctctaaa atccattgat ttcggcagtc cattacgacg
    47221 cagaatcccg ttagaatt
//
LOCUS       DAFFSV010000008        45726 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFFSV010000008 DAFFSV010000000
VERSION     DAFFSV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.008"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..161)
                     /locus_tag="LR110_000379"
     CDS             complement(<1..161)
                     /locus_tag="LR110_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL4821385.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINR"
     gene            complement(163..360)
                     /locus_tag="LR110_000380"
     CDS             complement(163..360)
                     /locus_tag="LR110_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBL4821386.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(754..2481)
                     /locus_tag="LR110_000381"
     CDS             complement(754..2481)
                     /locus_tag="LR110_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBL4821387.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2474..3667)
                     /locus_tag="LR110_000382"
     CDS             complement(2474..3667)
                     /locus_tag="LR110_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL4821388.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3854..4498)
                     /locus_tag="LR110_000383"
     CDS             complement(3854..4498)
                     /locus_tag="LR110_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4821389.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4592..4726)
                     /locus_tag="LR110_000384"
     CDS             complement(4592..4726)
                     /locus_tag="LR110_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBL4821390.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4728..6860)
                     /gene="feoB"
                     /locus_tag="LR110_000385"
     CDS             complement(4728..6860)
                     /gene="feoB"
                     /locus_tag="LR110_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBL4821391.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6857..7330)
                     /locus_tag="LR110_000386"
     CDS             complement(6857..7330)
                     /locus_tag="LR110_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBL4821392.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7731..7955
                     /gene="nrdH"
                     /locus_tag="LR110_000387"
     CDS             7731..7955
                     /gene="nrdH"
                     /locus_tag="LR110_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBL4821393.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8114..10273
                     /gene="nrdE"
                     /locus_tag="LR110_000388"
     CDS             8114..10273
                     /gene="nrdE"
                     /locus_tag="LR110_000388"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBL4821394.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10323..11288
                     /gene="nrdF"
                     /locus_tag="LR110_000389"
     CDS             10323..11288
                     /gene="nrdF"
                     /locus_tag="LR110_000389"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBL4821395.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11377..12516)
                     /locus_tag="LR110_000390"
     CDS             complement(11377..12516)
                     /locus_tag="LR110_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL4821396.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12825..13538)
                     /locus_tag="LR110_000391"
     CDS             complement(12825..13538)
                     /locus_tag="LR110_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBL4821397.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13792..14493)
                     /gene="nagB"
                     /locus_tag="LR110_000392"
     CDS             complement(13792..14493)
                     /gene="nagB"
                     /locus_tag="LR110_000392"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBL4821398.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14727..16259
                     /locus_tag="LR110_000393"
     CDS             14727..16259
                     /locus_tag="LR110_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL4821399.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16450..17652)
                     /locus_tag="LR110_000394"
     CDS             complement(16450..17652)
                     /locus_tag="LR110_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4821400.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17734..18423)
                     /locus_tag="LR110_000395"
     CDS             complement(17734..18423)
                     /locus_tag="LR110_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL4821401.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18529..18897)
                     /gene="rplL"
                     /locus_tag="LR110_000396"
     CDS             complement(18529..18897)
                     /gene="rplL"
                     /locus_tag="LR110_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBL4821402.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18950..19453)
                     /locus_tag="LR110_000397"
     CDS             complement(18950..19453)
                     /locus_tag="LR110_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBL4821403.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19657..19784)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19863..20552)
                     /gene="rplA"
                     /locus_tag="LR110_000398"
     CDS             complement(19863..20552)
                     /gene="rplA"
                     /locus_tag="LR110_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBL4821404.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20656..21078)
                     /gene="rplK"
                     /locus_tag="LR110_000399"
     CDS             complement(20656..21078)
                     /gene="rplK"
                     /locus_tag="LR110_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBL4821405.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21421..22089
                     /gene="sdaAB"
                     /locus_tag="LR110_000400"
     CDS             21421..22089
                     /gene="sdaAB"
                     /locus_tag="LR110_000400"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBL4821406.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22102..22974
                     /gene="sdaAA"
                     /locus_tag="LR110_000401"
     CDS             22102..22974
                     /gene="sdaAA"
                     /locus_tag="LR110_000401"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBL4821407.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23065..24084)
                     /locus_tag="LR110_000402"
     CDS             complement(23065..24084)
                     /locus_tag="LR110_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL4821408.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24257..25162)
                     /locus_tag="LR110_000403"
     CDS             complement(24257..25162)
                     /locus_tag="LR110_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL4821409.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25221..25766)
                     /gene="nusG"
                     /locus_tag="LR110_000404"
     CDS             complement(25221..25766)
                     /gene="nusG"
                     /locus_tag="LR110_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBL4821410.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25867..26037)
                     /gene="secE"
                     /locus_tag="LR110_000405"
     CDS             complement(25867..26037)
                     /gene="secE"
                     /locus_tag="LR110_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBL4821411.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26061..26213)
                     /gene="rpmG"
                     /locus_tag="LR110_000406"
     CDS             complement(26061..26213)
                     /gene="rpmG"
                     /locus_tag="LR110_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL4821412.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26375..27019
                     /locus_tag="LR110_000407"
     CDS             26375..27019
                     /locus_tag="LR110_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL4821413.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27075..27578)
                     /locus_tag="LR110_000408"
     CDS             complement(27075..27578)
                     /locus_tag="LR110_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL4821414.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27591..27637)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28175..29140
                     /locus_tag="LR110_000409"
     CDS             28175..29140
                     /locus_tag="LR110_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL4821415.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29239..30411)
                     /locus_tag="LR110_000410"
     CDS             complement(29239..30411)
                     /locus_tag="LR110_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4821416.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30398..31543)
                     /locus_tag="LR110_000411"
     CDS             complement(30398..31543)
                     /locus_tag="LR110_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4821417.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31557..32481)
                     /locus_tag="LR110_000412"
                     /pseudo
     CDS             complement(31557..32481)
                     /locus_tag="LR110_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32598..33677
                     /locus_tag="LR110_000413"
     CDS             32598..33677
                     /locus_tag="LR110_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL4821418.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33709..34302
                     /locus_tag="LR110_000414"
     CDS             33709..34302
                     /locus_tag="LR110_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4821419.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34412..34639)
                     /locus_tag="LR110_000415"
     CDS             complement(34412..34639)
                     /locus_tag="LR110_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL4821420.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34742..37330)
                     /locus_tag="LR110_000416"
     CDS             complement(34742..37330)
                     /locus_tag="LR110_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBL4821421.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37350..38009)
                     /locus_tag="LR110_000417"
     CDS             complement(37350..38009)
                     /locus_tag="LR110_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL4821422.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38058..38561)
                     /gene="trmL"
                     /locus_tag="LR110_000418"
     CDS             complement(38058..38561)
                     /gene="trmL"
                     /locus_tag="LR110_000418"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBL4821423.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38782..39648)
                     /locus_tag="LR110_000419"
     CDS             complement(38782..39648)
                     /locus_tag="LR110_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL4821424.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39757..40398
                     /locus_tag="LR110_000420"
     CDS             39757..40398
                     /locus_tag="LR110_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBL4821425.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40460..41821)
                     /gene="mgtE"
                     /locus_tag="LR110_000421"
     CDS             complement(40460..41821)
                     /gene="mgtE"
                     /locus_tag="LR110_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBL4821426.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41839..42738)
                     /locus_tag="LR110_000422"
     CDS             complement(41839..42738)
                     /locus_tag="LR110_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL4821427.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42740..43537)
                     /locus_tag="LR110_000423"
     CDS             complement(42740..43537)
                     /locus_tag="LR110_000423"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBL4821428.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43515..44219)
                     /locus_tag="LR110_000424"
     CDS             complement(43515..44219)
                     /locus_tag="LR110_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBL4821429.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44350..44925
                     /locus_tag="LR110_000425"
     CDS             44350..44925
                     /locus_tag="LR110_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBL4821430.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            45043..45720
                     /locus_tag="LR110_000426"
     CDS             45043..45720
                     /locus_tag="LR110_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL4821431.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT        13715 a         9587 c         8112 g        14312 t
ORIGIN      
        1 aatctattta ttaaaggacc gggatctaat tcatattttt gtttttctaa ttttaaatat
       61 accttttgta aaacctcaag aatatcagaa tatttaggat ctttacttat agatatatca
      121 ttatataatt catgaacaat ttgcttagca ttttttttca ttttagcact tccctggaat
      181 tgctccacct aaaaaaccac ctatagacat tcctgcaata caagtagttg ccttggccca
      241 atcgaccgta catttatttt tagtgcaata cactccattt ccataatatt ttccactatg
      301 agtggtaccc ccatagataa gttgtgtctc tttaatagac aaaattttta aatgtttcat
      361 aatctaatca cccctaaatt aatgttttta gactattatt ctaacattca ttaaatttca
      421 aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg aataagaatt taatcacttt
      481 taagtatata aactatgtat attctttaat attttttgag aatgcacttc gtacataata
      541 aactttaatc acattagtct ttcataccta cgatcgatct ctttgactcg cacccataaa
      601 ttcataatag tttgtcggcg tctttggacg tttcgaagat gagctacgca ttctcttttt
      661 ttatagacaa aaaagataga gacgaacatg gagcttccaa atttggatat ttgatccaat
      721 tttggaagct ccatttattt cgtgtctgtc tttttatttt ttccagcttt cgactaattc
      781 ttggttctct ttgatccagt cttttgctgc ttgctgtgga tcttttccat tgttgatttc
      841 caacataact ttttccatat ctttttctga ccaatggaaa ttgtctaata ctttgtaagc
      901 ttctggctgg tcttctttca atccttttcg aaccatcgta tggatctgct cttcgcttcc
      961 cattccgttt tcaggatctt ctaagtattt cagatcatat ttggcaaaca tccaatgagg
     1021 tgtccatcct gtaactacaa ttggttcgtg ttttttgatc gcttgaccta gtgcaacggt
     1081 cattgctcct gaagaagatg tctctacttt ccagtctttc aagttgtcat atttttggat
     1141 cgtgttttca gcagccgtaa ctacaccagc tcctggctcg attcctatga ttttcttacc
     1201 tgcctgatcg gttaactcat cgatcgaatt tacatccatg taggctggca ctgctaaacc
     1261 aacttttgct cctgtcaaat tggcacctag gtcttcgact tgatctttat attgtgcata
     1321 ttgtgaacca tgagttttag gtagccaagc tgaaaccatg gcatctgact cgccatttga
     1381 aacagatttc cacatgattg aattatctaa tggcgtcatc gttacatcat agcccatttg
     1441 tttcaatact tcaccgacta catttgttga agcaacttct gtatcccatt caacataaga
     1501 taagttgata cgttttgttt ctttggcaga tgaaaaactc cctataccaa tcgcacctaa
     1561 gatcactact actacagcac caatgatcag tccttgacgt tttttacttt tctgtgttgt
     1621 tgctcctgca gcttttttat tgtttggctg gttcaaatgc tgtgtaaaac ggtcaatgat
     1681 gattgctaag ataactaatg caaccccgtt gacaaatcca ttcccgactt gtgctctttg
     1741 caatgctgat aatacgccgc gtcctaagcc gggtgcaccg atcatagagg caataacgac
     1801 cattgaaagt gacagcattg ttgtttgatt gatccctgcc atgatcgtac ttttagctaa
     1861 aggcaattct agtttgaata gtttttgacg acttgtgctc ccgaatgaat cagaagcttc
     1921 tactaattct ttaggaactt gtcgaatccc taaattcgta aaacgaacgg ttggtggcag
     1981 agcaaagatg actgaggcaa atacccctgg aaccatcccg ataccgaaaa atgcaactgc
     2041 tggaatcaaa taaacgaatc ctggcattgt ctgcataaag tcaaggatcg gtttgataat
     2101 actttgggct ttactgcttt ttgccatcaa gatccctaat ggtaccccaa tgatgatcga
     2161 gatcacacta gataaaagaa caagtgtgac agtattcatc aaatcattcc atagaccttg
     2221 gttataaata aaccatagac cgattaatgt gaacaacgta agtcctggac gtttcttaga
     2281 aataaagaaa gctgctaccg tcaataacaa gataaacaaa ggagctggaa tcactaacag
     2341 taaatttgta atccccgaca tcactgcctg tccaatcgtt tgtaaaaatg aaaataggcc
     2401 agaaaaagtt gtcgtaaacc attctgtgat attttctacc cagtttgcca ctggtaattg
     2461 atagttcaat agattattca tgttcactca cctcgcttgt ttctgctaat gcttcaatca
     2521 cactacctcg gatgacgaca ccttttaatt ttccatcctc tacaactgca agtggagtcg
     2581 gtgagtcata aataatattg aaaatatcac ttaccaccat gtctttccca atcaccgtca
     2641 cattttcatc taggaagtcg atcaatggac gtttttgctt cctagcttct aacgcttgat
     2701 ctgcagtgat gatccctttc aggtgtctct ttctgtcaac agccatcagc atgctgactt
     2761 cttcattacg catccttgtc aatgcaacgt ttggtccatc agattcgata ttcgtcgtca
     2821 atgcaggtac catgatgttt tgagccgtaa gcactttgga acgatcgact tcttccacaa
     2881 attcacgtac ataatcattt gctggattgg tcaatatttc ttcacctgtt ccgatctgca
     2941 tgatttcgcc gtctttcatc aaggcgattc tgtccccaat ccgtaaagct tcattcaaat
     3001 catgggtaat gaaaataatc gttttttgaa cattggcttg caggtccagt aattcgtctt
     3061 gcatttctcg ccggatcagc ggatcaagag ccgagaacgc ttcgtccatc agcaagatct
     3121 caggatcatt tgctaaagct ctggctaggc caacccgttg ttgcatccca ccagataact
     3181 gactaggata ttggtcttta aatgaaagca aactagaatt ttctaatgct ttttctgctt
     3241 tttcttgtcg ttcttcttta ggtacaccac ggacttctaa cccgtattct gtattttcca
     3301 agatcgttcg atgtggaaac agaccgaagt tttggaaaac catattgatt ttatgacgac
     3361 ggacttctcg caattcttct ttgcttaact tcgatacatc ttctccatca atatagatac
     3421 tgcccgaagt tggctcaatc agacgattca atagacggat caatgtggat ttcccactcc
     3481 cggaaagccc catgatcacg aagatttctc cttcttttac gtcaaaattc acgtcataca
     3541 caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt cggctggtga tcattcatca
     3601 tatctaaagc ggcttgtgtc tttttaccaa atatctttgt taaatgtttt acttctactt
     3661 ttaccaaaca aattctcctt ttttatgtaa attcattttt ctttctgaaa aaagtgacca
     3721 cactatgtta acatcatgta gtcactttgt caaaaatgaa agaaatttgc ttgtccaaac
     3781 tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta gccaagttaa tattttttta
     3841 atatttcaca caatcattct gcaagctgat gctccgggta aaaaaagttt gtcatacgct
     3901 gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc tgccgtaaat ttagcatagg
     3961 ggccaggtga aacaagcagt tcttcattat gagaaattgc gataactgtg gcgccagtcg
     4021 cctgccagat attcaactcg ctaattgaac ggtccagatg cattgctttt tctgataaag
     4081 ataattcaaa cggaagtaaa ggattcatct tatgtattcg cttcgtttga ttgaccagct
     4141 gatccaataa ttgagaaaaa tgattcagct catcttgttg cttctccaca cttgccatga
     4201 tttctgcttt tgtatcttgg atggattgta catcttgata ttgcaatagg aaatcttttg
     4261 ctttttcctt cgaagcaaca aatgccccac ttccatgccg tacttccatg attccaagat
     4321 ctaccagcac attgatagcc ttgcgggtag tctcaggaga agcattgtac gtactggcaa
     4381 gtgttgagcg ggcatggatc ttctcaccca ctttatatcg gttttcaacg atacgttcgg
     4441 ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat ttttttcttt ccagacattt
     4501 tctttcctct ttcttttttt actttccctc atcttactat gctctagcaa aataagcaaa
     4561 aaaggggaga atagcctctt gcaacgactg atcatcgctt gatctgttcc ttttttactg
     4621 ggcaagctgt ttgacagtca tcacaattct taccggattt taaacgagta tagacaattg
     4681 cgcctgcact tccaaagatt aacacactta ataagaatgt cgccatccta ttcctctcct
     4741 tctaatgaca atatagtata aaactggttc ttggattttg gctgacggaa caaaacataa
     4801 acgagtccta tcatcagtaa aatagcaaga tacgtcccta acgctacctg tccaccctcc
     4861 aataagtgtc ccaactgata aatcacaaaa ctaactaaat aagctaaacc gcattgatag
     4921 cctatggcga tccaagtcca tttcttggca ttcatttccc gatggatagc cccaattgcc
     4981 gcaaaacaag gcgcacaaag taaattgaag gctaacaatg aataagctgc tgcgggagta
     5041 tatgtgtgtt gtagcacttg ccagatttct ctgccatttt cggatacttc cgcatgacca
     5101 aacaaaatac caaatgtgcc aataatattt tctttagcga ccaatcctgt aatcgctgcg
     5161 actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa caactgccag gattcgcccg
     5221 aaaaaggcta agatactttg gtcttcagaa acaggttgag cataaaaatt ataagaagag
     5281 ctgaaccaga tcacgatact agaaacaaag ataatcgttc ctgctttttt aacaaaagac
     5341 ttgctgcggt cataggtttg tcttaggacc gctgaccatt gaggaaaatg atatgcgggc
     5401 aattccatga taaaaggagc tgtatctcca gcaaacaaac gtgttttttt caagctgatt
     5461 ccagagaaaa taattgcagt cataccaaga aaataggcag aaggagctac ccaagattga
     5521 tgaggaaaaa aggcccctgc tactaaggca ataatcggca actttgcaga acaaggcatg
     5581 aaggttgtga ccatgatcgt caaacgacga tcttgctcgt tttcaatcgt gcgactggcc
     5641 atgacgcctg gcactccaca tcctgtagca atcagcatag gaatgaagga ttttcctgaa
     5701 agaccgaatt tcctgaaaag tcgatccatg acaaaagcga tcctcgccat atatccgcag
     5761 tcttccagaa agctcaagca taaaaacaat ataatcaatt gcggtagaaa tcctaacact
     5821 gctcccacgc ctgcgatgat gccgttcaag atcagctcct gcatccatcc cgctacttgc
     5881 cattcagcta acaatctgcc aacatgttct ggtaaccatg taccaaacaa tgtgtcattg
     5941 atccaatctg tccccattgt tcctactgtt tgaatagcca gataatagat cagccacatc
     6001 acaaatgcga agatcggcaa ggctaaccaa cgattcgttg caatctgatc gattttgtca
     6061 ctcatcgata attggaaaga atcatttgac acaacacaga gtgtacatag ttgggtaata
     6121 aattcataac gttcattgac cagaatcgtc tcactatcgt cgtccaatag tttctctgtt
     6181 aactggatca gttgctcgat ctctttttgc tgagaaactg ctatctcgta atcctctctt
     6241 accttctgat ctcgttcaaa atatttcatt gcataccagc gtttcttgcc gctttgttct
     6301 cctagtacat cttcaatttc atgtaaagct gcttctaaac gtggatcata tgtacaggga
     6361 acaggaatag aaggccgctt ttttgcagac tcctgaatct tttcgattag tttttcgatt
     6421 ccttgctttt tcaatacgct gattggtaaa actggtacac caagaccata ggaaagcttt
     6481 tctagattga ttttttttcc ttcttttttg atgacatcca tcatattcag accaatgatc
     6541 ataggaatgt ctaaatccaa taactgcgta gttaaataaa gatttcgttc taaattggta
     6601 gcatcaataa tgttgatgat cagttcaggc tgttcctgaa ccaaataatc tcttgcaatc
     6661 acttcttctg gtgtataagg agacaaagaa tagatgccgg gtaggtcctg aatatgaatg
     6721 ttcggatttt ttttcaacga tccttctttt ttttctactg ttacgcccgg ccaattccct
     6781 acatattgat tcgaaccagt caagagattg aacaagctag ttttcccgct gtttggattt
     6841 ccgactaacg cgatattcat tctttttcct ccctttgtat caagattagt tcggcctctg
     6901 actttcgtaa cgtcagttca taccctcgca ctgtcagttc gattggatct cccagagggg
     6961 ctagttttcg gataactatt tttacatttc gcgtcaatcc catatccatt aaccgtcgct
     7021 tcactgcgcc ttgtccgtgg attgctacga cgatcccagt ctctcctgct tttaattgat
     7081 caagagaagt ccagctttca gaggaagcag tctttttatt gacaaagatt tgacctagta
     7141 tttcccgact tactgccact cgatgacttt ttaccagtaa aatggcattg ttgcctgata
     7201 aattgacgac gacgatttta cttccggcaa cgatcccgat attttgcaaa tggcgttttg
     7261 ttttttcttc tgtttcgatt cgttcgactt gataaacgaa attctgcttt gcctgcgcta
     7321 aggtctgcat attgattcct ctcttgtgaa ataaatcatg tattaaaatc attcattata
     7381 tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat cattctcaca tagaatactc
     7441 tcggtaattg agaatgtcaa ttatacttgt gatattatga gcgaatcttt agtattgata
     7501 gataaaaatg agaaaaaaat atttcttcat agttttccac aattttttct ctattaaatt
     7561 tgctattttc ccacacgata cttgttttca gctctttttt caaaaacaca aaacttagta
     7621 ttccacagtt tccacaacac aatatgtagt gtttgttgtt tgactttttt actaagagtt
     7681 ttatactatc tattatagat aataagaatg tgttagagga ggcactcacc atgaacgtaa
     7741 aaatcttttc taaaaataat tgtatgcaat gtaagatggt caaacggttt ttaacagaaa
     7801 atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc tgaagcagta gactggttga
     7861 aagcacaagg attccaaagt gttcctgtca ttacttctga tgcagaaaca gttatcggat
     7921 tccgtcctga tcagttgcgt aaacttgcca gctaacatga ctaaaagaat caaaacgtga
     7981 tacatggatt tgaagggctt gagacagcac cagtttttgt cgcaaacttc caaatccttt
     8041 tttaaagctt aaaaacaatg caatgatttg caatcaagca tcatcaaata cgaaatgaag
     8101 gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc agttatttca agctgaataa
     8161 tgaaatcaat cggccagtca atggtcaaat ccctttgaat aaggataaag aagctttagt
     8221 tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact tttctatcta ttatggaaaa
     8281 aatcaattac ttgatcgatc aagaatatat agaagcagaa ttcattcaat tgtattcacc
     8341 atcatttatt gaaaagctat atcaattttt ggacgagcaa catttttctt tcaaatcttt
     8401 tatggctgct tacaaatttt attctcaata tgcgttgaaa accaacgacg gttccgctta
     8461 tctggaaact tatgaagatc gagtagcctt caatgcgctt tactttgcga acggggatga
     8521 agaattagca cttgctcttg cagacgaaat gatccatcaa cgatatcagc cagctacacc
     8581 ttctttcttg aatgctggac gtaaacgtcg gggcgaattg gtgtcttgtt tcttgactca
     8641 agtcactgac gatatgaact ctatcgggcg ctcaatcaac tctgctttgc aattgtcccg
     8701 tattggtggc ggcgtaggga ttactttatc aaatcttcgt gaagccggcg ctcctatcaa
     8761 aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg aaattattcg aagatagttt
     8821 cagctattct aatcaattag gtcagcgtca aggagctggc gtggtttatt tgaacgtttt
     8881 ccatccagac attattgctt tcctttcagc aaaaaaagaa aatgcagatg aaaagattcg
     8941 cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa ttttatgaat tgacacgtaa
     9001 aaacgaagat atgtatttat ttagtccata tagtgtcgaa aaagaatatg gtgtcccatt
     9061 ctcttatgta aatattacag ctgagtatga tcatctagtt gcaaatccaa acattcgcaa
     9121 acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca aaattacaac aagaatctgg
     9181 ctatccttac gtaatcaata ttgatacagc gaaccgcgag aatccaatcg atggcaagat
     9241 catcatgagt aatttatgtt cagagatttt acaagttcaa acgccttctg tgattaacgg
     9301 caaacaggaa tatgaagtat taggaacgga tatctcatgt aacttaggct cgacaaatat
     9361 cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg cgtacgatga ctcgtgcatt
     9421 gacatttatt acagatgcat ctgatatcga tgtcgtacca ccgatccaaa acggaaataa
     9481 gctgaatcat acgattggat taggtgccat gggcttacac acttatcttg caaaagaaca
     9541 aatggaatat ggctcagagg attcattaga ctttacaaat atttacttca tgctgttgaa
     9601 ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa agaaaacaag ttttccataa
     9661 ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa aaatacgtaa ctggagaatt
     9721 ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc atttttatcc caactgctga
     9781 agattggaat gctttgaaac aagcagtaat gaaagatgga ctgtatcatc aaaatcgctt
     9841 ggcagtagca ccaaatggct caatttcata tatcaacgat acgagtgcaa gtattcatcc
     9901 tatcactcgc atgatcgaag aacgccaaga gaagaaaatc ggtaaaattt attacccagc
     9961 gccttatcta tccaatgaga cgattccgta ttacacttct gcttatgata tggacatgcg
    10021 taaagtaatc gatgtttatg caatggcaca acaacacgtc gatcaaggca tgagtttgac
    10081 tttattcatg cgttctgaaa tcccagaagg catttatgaa tggaagacaa caacgaagca
    10141 aacgacacgt gatttgaata ttttaagaca ttacgctttc cacaaaggaa tcaaatccat
    10201 ttattacgta cgtaccttta ctgatgatgc agaagaaatc ggaagcaatc aatgtgaaag
    10261 ctgcgtgatc taatatcata gatagattcg cttattcata ttttaattaa aggagcattc
    10321 tcatgtcaga aacatattat gaagcaataa actggaatga aattgaagat atcattgata
    10381 aatccacttg ggagaagctg acagagcaat tttggctaga tacacgtatc ccactttcaa
    10441 atgacctaga tgattggcgt acattgtctc aattggaaaa agatacagta ggacatgtat
    10501 ttggcggttt gacactgcta gatactgtcc aatctgaaag tggcatggat cagttgcgta
    10561 aagatgtccg cactccccat gaagaagccg ttttgaacaa tattcaattc atggaatccg
    10621 ttcatgccaa aagttattct tctattttta gtacactgaa tacgaaaaaa gaaatcgaag
    10681 aaatttttga ctggacaaat acgaatcctt atctgcaaaa aaaagctgag cgtatcaatg
    10741 aaatctacaa acacggtaca ccgctagaaa agaaaattgc cagcgtcttt ttagaaacat
    10801 tcttgttcta ttcaggtttt tatactcctc tttattattt aggaaacaac aaattggcta
    10861 acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc tgtacatgga acctacatcg
    10921 gttacaaatt ccaattagga ttcaacgagt tacctgaagc agaacaagaa acgttaaaag
    10981 actggatgta caatcttctt tatgagcttt acgaaaacga agaacgttat acagaagaat
    11041 tatacgatcc aatcggctgg acagaagaag tcaagacatt cctacgctat aatgccaaca
    11101 aagcattgat gaacctaggg atggatccac tctttccaga tactgccaat gatgtcaacc
    11161 caatcgtaat gaacgggatt tcaacaggca caagcaacca tgacttcttc tcacaagtcg
    11221 gaaacggcta tttgttaggt catgtagaag cgatgaaaga cgacgattat ttgattggat
    11281 tggaataagg atttatttta attcaaaaat ttttcgaaaa ataaaaagag tttagcgtac
    11341 agatatttta aatgtacact gaactctttt ttatgcttat tcacttgttt cttccagcac
    11401 ttttggactg tctaactttt ccgaaacatg ttccattctt ctcaataaat agattttccg
    11461 gatatttaat attatcgtct taatcactgc atagacaggt atgcaaatca gcatgcctaa
    11521 tattcctgcg acacttcctg ctccaatcaa taccaagatg atcgtcaaag gatggatatt
    11581 cattgatttc ccaaataaca acggcttgat caaatttcca tctaattgtt ggatcactaa
    11641 aatcgacaac gccatataca aagcttgaaa tggagagata aacaaaccga caatgattgc
    11701 tggtaccgct ccaataaaag ggccgacata cgggataata ttcgtgattc cgcaaatgac
    11761 cgcaagcaac aagctataag gctgatcgaa tattctcatt gccaaaaagc tcattacacc
    11821 aataatcaaa gcatctaaga tcgtactgct gatataggca gataatgttt cgtttaattc
    11881 ttttaccgtt tggcgtaact ctttacgaat cgctttcgga aagaagtgag acaacgcatc
    11941 aataaatttg tgcccatcct tgaacataaa tagcaaaata aatggaacgg taaataacaa
    12001 aataaaaaat tgcatcataa atgaaatgat ttttgagaca cttgcagata aactgactaa
    12061 tatgatattg aacaaattac taagagataa atttgctgat gctaattgtt cttccaaatt
    12121 gaaattttta aactcttccc gctgcgccaa ttcatttaac catttgcttg tttcatctgc
    12181 atacattggc aagctctggg tcaattgaat tgtctgttcc attaactgag gcactacatt
    12241 catgacagtt aaaacaacta aaatgattac gaccaaaaac gtcaataaaa aaccgaacac
    12301 tctcgggatc cgatgctttt cgagaaaaac aacgattgga tcgaacatat aatagataaa
    12361 accagcaaca agcaatggta aaaaaactgc cgaaagcatt ttcaatattg gactaaaaat
    12421 attcggcatt tgtaaaataa agtacacgcc gatgatcgtg agtactagct ccactgtcca
    12481 gaaaaataat tttgattgtt taaaacgtga ttgcatacat ctcctccaag gtcctttgat
    12541 acttctattc tatcatttaa caaagagtta cgcgctgaga ttttcatttg cttaaaaaaa
    12601 acaaaaaaaa taaacatatt ttaatgagta gacgagtact taaacaaaaa cacggtttgg
    12661 agtagtgaca aaaattatac tgcagtccct ttttccaaat aaacggtgtt caaaagcttg
    12721 catcttcgta ataagcccag ccaaacgaaa aatataagga actattttta tttggctggg
    12781 cttattactt gatgtaggac atctatttta caacctcaga tcatttattg cgcaatactg
    12841 actaaatgca actttgtgat atttttgtgt acggacaact cctcgatcac atcggcataa
    12901 gtgagccctt ttggaagatc aatcgtatag atatttttgt acactcggta gtcatcaacg
    12961 aattttacgt caaaattcac atcttcgatt acgatatttt tctcttcaaa ataatcggta
    13021 agaaaatctt tggtttcttt tctatggata aattggattt ctaacttttt agtcgtaggt
    13081 acatggatca cgcgtttcac tactgtgagt gcaatagcaa tcccgataaa actgatgatc
    13141 gcaatggaat agtaacccat gccgatggcg atccctaatc cagcaattgc ccataaagaa
    13201 gcggcagtcg taagtcccgt caccgattgt ttcgtgacga tgatcgttcc cgcgcctaag
    13261 aaaccgacac cactcaccac ttgagcaatc agccttgctt catctgaacg aatcgttcca
    13321 gcaagctgtg gatactctaa agcatctctt aatgcaccac tagcgatctc aacttggatc
    13381 aatgcaatga tacatgctcc catacaaact aaaatatgcg tacgcatacc tgctggacga
    13441 tttttatatt gtcgttcaaa accgattgct ccgccaaata gcatagcgat ccctaaacgt
    13501 aaaataatct ctggaattgt caataccata tcattcatct aaacaccctc cagtccaata
    13561 tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt tacctcttac gaaaaaagga
    13621 ctgtgacaaa atttttgtca tagtcctttt tatgaataaa cggtgttcaa tcagctgacc
    13681 ctcggtatta ctcaacaaac ggcaaaatat ggggagctat ttttcctttg tcttctctaa
    13741 tactcaggtc aaaacgctga tttcacaacc tctttagcct actattgaaa attacaactt
    13801 acttgctgct tcttcatcga tgatcacaac aacattgtcg tgattttgca atacacttgc
    13861 tggcaaatga ttcgttacag gtccttcaat catccctttg attgcatcag ctttgctttc
    13921 gccaaatgcc atcaagacga tttctttact tttcataatt gaaccgattc ccattgaaac
    13981 agcttttgtt ggtacatctt ctgctttatc gaagaatcgt ttgtttgcat tgattgttga
    14041 ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa gcaccaggtt cattgaaacc
    14101 gatatgtcca ttttgcccga ttcctaagat ttggatatcg attggatgtg catcgatgat
    14161 ttgatcatac tttttgcatg ctgcttccag atcttctgct tttccatctg gtacaaacgt
    14221 ttctttgaat ggttttttat caaataattg gacatccatg aaatgacggt agctttgttc
    14281 atctgaaccg cctagtccaa cgtactcatc taagtttaca gaagtcattt cactgaaatc
    14341 caaatcgctt gaagtcattt ctttatataa agtaactggt gtgcttcctg ttgccaatcc
    14401 taatactttt gcacctttgt tcataccttc tttgatgatt tcaaatgctt tttttccgcc
    14461 ttcttcagcg ttttttacac gaatgatttc catagtgatt gctcctttat attttggtat
    14521 agcccaatta tagcattctt tttttctttt tacaataatt agtatagacc aaattgcatt
    14581 tttttcattt tcttgacaat aaataggaat ccgtgttagg tttcataaga tggttcaatc
    14641 aaacaatcca tgttgcatga aaaccaagca agtgattcac tgtggacgct ttatgtctgc
    14701 agttttttta tgaaaaagga gttattatga gtatactgac attaaacaac attacccagc
    14761 aatttggcga caaaatcctc tatgaaggtg tcgatctgca attaaatgct ggcgaacatc
    14821 ttgggctgat tggacaaaac ggagccggta aaagtacatt gattaaaatc atcactggtg
    14881 agatcttacc agatgacgga cagatccatt ggcaaaaaaa tattcacaaa ggttacctcg
    14941 atcaatatgt ggaagtcgat gaaacattaa caatcgaaga atttctaaaa actgcgtacg
    15001 caaaagaata cgaaaaggaa gaaaaaattg cttttcttta ccaaacatat agcgagacct
    15061 tcgatgattc cttgctggaa aaagctggga aactccaaac ggaattagac caaggagtgt
    15121 tttatcaaat cgacacctta gttgctgaga tgagcagcgg gttggggatc gatgtcttag
    15181 gtttagattc actgttgaaa aatctaagtg gtggacaacg ttcaaaggtg attttagcaa
    15241 aactactttt agaaaaccca gatgttctga tcttagatga accaaccaat cacttagatg
    15301 atcagcatat ccaatggctg acgcagtttc ttcaggattt tgacggtgcg tatatcgtga
    15361 tttcccatga ccaagaattt cttgatcaga tcaccaccca tatcgcagac atcgagtttg
    15421 gaaagattac taagtatact ggccatctaa aacaagcatt gaaacaaaag gaacaaaatc
    15481 gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca aatcgaaaag accgaagctt
    15541 atattcgaaa atacaaagcc ggttcccgat caacgatggc aaaaagccga caaaaacaat
    15601 tagataaaat cgaacggctg acgcctccag cttcagcaag taaagctgtc tttgattttc
    15661 cattctcgcc tattgtcacg acattagcag tggaaacaac tgatttagtc atcggatatg
    15721 atcgaccgct acttgcaccc atcaatttga ccatccgctt tggtgaaaat attgccattc
    15781 gaggcttcaa cgggattgga aaatctactt tattaaaaac actgatcggt gagattgaaa
    15841 aaataagtgg cgatttccac ttcccagata acacaaaaat caattacttt tcacaagatc
    15901 tgtactggga aaatccatta gaaacaccac tacaatattt aagcagtcaa tttccaaaag
    15961 cgactatcaa agaattacgt cgccaactag ctaaagcagg gttagttaat caattagcaa
    16021 gtgaacctct cactacatta agtggtgggg aacaaactaa ggtgaaactt gcacagttga
    16081 caatgaacca aggtaattta ctgattctcg atgaaccgac gaaccatatc gatcaagaaa
    16141 cgaaagaaag cttacaagac gggatacaga aatttccagg aacagtaatc atcgtctctc
    16201 atgaacaaga attttatcaa gatcttgtgc aacgagtcat tgagattgaa ccaaaataaa
    16261 cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa gttttgtcac aactccttct
    16321 tctgaataaa tggtggccaa ttagctgacc ttcggtatta gctcaaaaaa cggcaaaata
    16381 tgaggagcta tttttatttg gctattctta ttacttgatg cagaacagct tttcacaacc
    16441 tcttttcgtc tattttttat tttcgagatt tctctttcct ttaaaaacaa ctgtttgaaa
    16501 gaagaacgca catccttgaa aaacaagccc gctaaccgtt acacctaacc agccatactg
    16561 ctgccacatt gtcgtcccta gcaatgaacc agccgcacct ccaataaagt acataaacat
    16621 aaatacagta ttatttcggt tgcttgctgc ttcacctagc ccttgtactc gtgtttgatt
    16681 ggcgacttga ccaaattgat tcccgacgtc taataaaata atagctaaaa gcaaaatggc
    16741 caaatggctt ccgccaacca ataaagcaat aaagcttatt gtttgcataa caagtcccat
    16801 atacacgatt tttctttcag aataagaatc tgccagtcgt cccacgatag gtgcagcaac
    16861 tgctccagac aaaccaaaaa tcgctaatat ccctgcttcg aatgttcccc aattgtaggc
    16921 gggactgctg atataaaaaa tcaacgtaga ccaaaagatc gaaaatgttc caaacatgaa
    16981 aaatccactg actgccgctt ctcttaatag acgttgagaa gcaactaaat gcggcaaact
    17041 tttaagtgaa tcaaggtatg aaacagctgt actactttgc tggacagaac gcggcatttt
    17101 ccagtaaagc agaagtgtaa gaattccgac tgcacataaa gcaaataaat agatcatccg
    17161 ccaagaaaaa agactggcaa caataccaga taccgtccga gaaagcaaaa tccccacgag
    17221 taaaccgcta agcaatgtac ccatgacctt ccctcttgct tttggtcccg ctagtaccgc
    17281 accgtaagga atgatgattt gcggcacgat cgaaagtaat cctattaaaa aagaagcgca
    17341 tgcaaataac gtaaacgaag gcgcaaaaaa agcggccaac agagagacgg cagataacgc
    17401 tgccattcga atgatcaatt ttggacgatc caccacgtcc cctaaaggaa ctaaaaacag
    17461 taaccctaac gcatagccta attgggtcaa catcgtcaat atcccgactg cacttgtgct
    17521 aacagataaa ctagctgcta cttttgttcc aatcggctgg atgtaataca tattggcaac
    17581 aacaacgcca caagtgattg ataatagtaa aataagcgaa gttgttaaag gttttttttc
    17641 attactcttc attgatttcc cccatttcct atcttagttt acaacttatg gaaaagattg
    17701 gaaatagaaa aatacaacta ttcgccacac ttattatttg tgcttttgtt ttccttgttt
    17761 tttcttgcga tctttataaa tgaagacaac taaccctact agcacagtaa cacttgcaac
    17821 caacgtgact gcagttttgt tatatccgtg aaacagagct tctccaccac acgcatacaa
    17881 cacagacgaa gggatgactc caatcacgat cgacaatatg atttgctgta gaggcaattt
    17941 catcgcatct gcagcaaaat tgaccataga agatggaata accggtatca tatagcctaa
    18001 cattacacct atttttggat gtttcatttg tcggattgct tgcacccaat gattcgtttc
    18061 ggttttctta tctaaaaatt ttaagcgatg cataaggaaa attgcagtga tgttaccaag
    18121 agcattaccc aaaatgttga tgatcgcacc tgtcagtggt ccaaagctca aaccagcaat
    18181 gactccaact actgaagtag gaatacctgg aacagcacac atcaccgctg tcaatacgat
    18241 caagagtatc gatgcaaaag tcccatgcga acgaatatgt gcgagtagct ctgccttact
    18301 cgctctcgga tttaaaaata attggatgtc tgtctgatat tctaaataca accgatagcc
    18361 cactaccagt atcagaatga ttcccaacga gatcaatatc aatcggatta ttttcttttt
    18421 catatttcct cctgaaaaaa atctatattc attaaataat agtttaacat aaaaacaaaa
    18481 aagcaccgaa atccaacata ggatctcagt gcttttaaat agtaaaaatt atttaacagt
    18541 tacagatgcg ccaacttctt caagtttagc tttcaattct tcagcttctt ctttagatac
    18601 gccttcttta actggtgcag gagcgccatc aactacagct ttagcttctt tcaagcctaa
    18661 accagttgct tcacgaactg ctttgataac tttaactttt tggtctccag ctgcagttaa
    18721 ttctacagtg aattctgttt gttcttcagc agcagcagcg ccaccagcag cagctacagc
    18781 tacaggagca gcagcagata cgccaaattc ttcttcaata gctttaacta ggtcgttaag
    18841 ttctaggata gttgcttctt ttaactcagc aacgatgttt tcaatgttca atgccattat
    18901 aatttcctcc gtttaattgt tcttttttat ttaatgcttt atgcggcaat taagctgcat
    18961 ctccatcttc tttgtctgcc actgctttga cagcgtaagc cacgttgcgg actggcgctt
    19021 gtagtacaga tagcaacata gatagaagtc cttcgcgatt tggcaatttt gccaatgctg
    19081 tgatttcttc caatgaagag actttacctt cgatgatacc gcctttgatt tctaatgctt
    19141 ttgcttcttt agcaaattcg tccatgattt tcgctggtgc gactacatct tcgttactga
    19201 atgcaacagc tgtaggtcca gtgaatactt cgtccatgcc ttcaaggcca gcttttttag
    19261 ctgcacgtga aagaatagaa tttttgataa ctttcatttc tacgttagca tcacgaagtt
    19321 gtttacgtaa acgtgttact tcatcaactg ttaatccgcg gtagtcaacg actactactg
    19381 atgcagctgc tgtaaatttt tcgcttactt catcaacgat tgctgctttt tttgcgataa
    19441 ttgcttcact catttttgag tttcacctcc tgaattttga tggaagagtt ttttgcactg
    19501 aaaaggcaag tcacttttat agttactttg ctcaaccagt attattcatc acctattcac
    19561 ttcgttcata gtgattctac tcgctttgct cgaaaggaaa ctcctttcgc tttgctcaac
    19621 cagtattgtt catcatctct tcacttcgtt catagtgatt cacaataaaa aactctatgc
    19681 caccgcagac atagagggac tattgaatat attcaataaa cgtcctcggt aggaaattaa
    19741 ggcttgatgc cacctactgt cttcggtaca agttaattat taacctcaac tagcttagca
    19801 tgctaaacta gttgagtcaa gaattatttt tcagttggat caatcaacat tctaatattt
    19861 aattagaaag aagcttggtc aacatggata ccaggtccaa atgttgttgt gatcgttaca
    19921 tttttcatgt attgaccttt agctgctgat ggtttagctt ttagcaatac atcatggatt
    19981 gctgcaaagt tttcaactaa tttttcatcg tcaaatgaaa cttttccgat tggtacatgg
    20041 atatttccag ctttgtccac acggtaagtt actttaccag ctttcacttc gtttactgct
    20101 tttgttacgt ccattgtaac tgtaccagtt ttagggtttg gcattaagcc ttttggtcct
    20161 aatactcgtc ctagacgacc tacagtagcc atcatatcag gtgttgcaac aactacatca
    20221 aagtcaaacc atccgttttg gattttttga accatgtcgt cgtcacctac gaaatctgca
    20281 ccagctgctt cagcttcttt tgctttgtct cctttagcaa aaactaaaac agtttgtgtt
    20341 ttaccagttc cgtttggtaa tacaacagca ccacggattt gttggtctgc ttttttaggg
    20401 tctacgttca aacggtatgc aacttcaact gttgcatcaa attttgcaat gtttgtttct
    20461 tttgctaaag ctactgcttc ggcaacagga taaactttac ttgaatcaac tttttttaat
    20521 gcatcttgca tttttttgct ctttttagcc attttggttc ctccttgatt gtggttataa
    20581 cggttgtacc tcccacgtgt tctatatctt tcaatataga gccaactgag aagctgctgt
    20641 cttagggtgt atagattatt ctacagtgat ccccatgctt cgtgcagttc cttcaaccat
    20701 gcgcattgct gcttcaacgt tagctgcgtt taggtcttcc atttttaatt cagcgatttc
    20761 tcttacttga tcgctagaaa ctttcgcaac tttatttttg tttggttcgc ctgaaccttt
    20821 ttcgatttta gctgcttttt tcaatagaac agcagctggc ggtgtttttg taacgaatgt
    20881 gaatgaacgg tcttcgtata cagaaataac aaccggaatg atcaaacctg cttgatcagc
    20941 tgtacgagcg ttgaattctt ttgtgaatcc catgatattg atacccgctt gacctagtgc
    21001 aggacctact ggtggagctg gagttgcttt acctgcagga atttgcaatt taacgatttt
    21061 ttctactttc tttgccacga gacatacctc cttaagtccg tgatgtggtt aattggagat
    21121 gcatatttct cctcccacgt tttatctgat ctgaaaaaat ccagacaaat aatgtgcaaa
    21181 acaatgcaca ctggtacagt atagcaaatt tatttgtttt ttcaagattt ttttctgcta
    21241 tttttacatt ttgcttgaat aaacgctaaa tttattctaa aaaaacaaca atttttcgtt
    21301 ctcatttctt cttttatgtt acacttgcgc tagcatggtt caacagatga aagcaaacaa
    21361 taacacggtt ttttagaccc ttgtacaaag attcctacat aacaaaatga ggtggagaaa
    21421 atgaaagatt taaggtatag aagtgtcttt gatattattg gacctgtgat gattggtcca
    21481 agtagctctc acactgccgg agctgctaga atcgggaaaa tcatacgtag tatatttggc
    21541 gaacagcctg attctgtaga tatttatcta tatgaatcat ttgcaaaaac ctatcggggg
    21601 cacggaacag atattgcatt agttggcggc ctgttaggga tggaacccga tgatgaacgt
    21661 ttagctgatt ctttgaaatt ggcatatgaa gccggtatgg aagtcttgtt tgttcctaag
    21721 agtgaaaaag ccgaacatcc aaactctgta aagatgcttc taaaaaaagg aacacgaaag
    21781 ctttcagtca ctggcatttc gatcggcggt ggcaatatcc agatttctga attgaatgga
    21841 ttcaaaattt ctttaagcat gggtactcct accttgatcg ttgtccacca agatgttcca
    21901 ggtatgattg ctaaagtgac tagtatactt tctgagacca atacgaatat cggcactatg
    21961 acagtgacac gggaatcaaa aggtgaaaat gccattatga taattgaagt agatgatcct
    22021 caagtagaag aaacggtaaa aagactcaaa cagctgccaa atatcgacag cgtaaattat
    22081 ttcgattaag gagctaaaaa aatgttttta tcgattgaag aattagtaga tcaagcaaaa
    22141 gcttatccaa gtgtagcaga attgatgatt gcagttgaaa tggaaatgag tggccgttcc
    22201 cgtagtcaca tcatcgagat catggaaaaa aacctctcag taatggaaca atcaatcaac
    22261 gagggaaaag aaggtgttac ctccgtcaca ggaattaccg gtggtgatgc aaaaaaatta
    22321 gatgattatt taaatgaggc agacttttta agtggagaaa caatcttaaa agctgtacgc
    22381 aatgctgtag ctgttaatga agtcaatgca aaaatggggt tgatttgtgc tacaccgact
    22441 gccggaagcg cgggtgttgt tcctggtgtg ctgatggctg tccgagatcg gcttgatttg
    22501 acacatgaac agcaattgga attcttattt accgctggcg ctttcgggtt agtcatcgca
    22561 aacaacgctt ctatcagtgg tgcagaaggg ggatgtcaag cagaagttgg ttctgcaagt
    22621 gcaatggcag cagcagcttt ggtttgtgca aaaaatggga ctcctcatca agcagcacaa
    22681 gcagtagcca ttacattgaa aaatatgatg ggactgattt gcgatcccgt tgcaggctta
    22741 gttgaagtcc cgtgtgtcaa acgtaatgct ttaggctctt ctcaagcttt tatctctgct
    22801 gatatggcat tagcgggtat cgaaagtgtc attccgccgg atgaagtcgt tgctgcaatg
    22861 taccaagtcg gacgtcaaat gccgtcgatt ttcaaagaaa cagcagaagg tggcttagct
    22921 gttacgccaa ctgctaaacg acttgcggaa gagattttaa ataagagggc gtgataaagc
    22981 tccttaattt cgagtattaa gagattagtt atcttcataa caaaaaacga agagaggact
    23041 aattgccctt ctcttcgttt tttattaatc catcaatttc gtatttttta cttcatcgat
    23101 cgtttttgtt cctgcaagtt gcatcgtgat cgacaattct ttgttcaggt gttcaaaaac
    23161 agatgttact ccttctgccc cacctagatt caacccataa atcactggtc ggccaatcgc
    23221 aactaagtca gcaccacttg ccagagcttt gaagacatgc tctcctcgac ggacaccaga
    23281 gtcgaaaatc actggtacac gtttgttgac tacttcggca atttttggca atacttcaaa
    23341 ggacgcagga cctccatcta gttgacgtcc accatgattg gatacccaga taccatctgc
    23401 ccctgcagag atagcgatta ctgcatcttc tggcgattgt attcctttta cgataactgg
    23461 aagatgggtg atttctttga ttgttttgat gtcatctggt gtcaagcctt gttttgcagc
    23521 tgcataaatt tctgcgattc cttttccttc gccattacca cttgcacttt gttcgctata
    23581 cgcagctaag ttaggcattg gtaatggaaa ttggaattga ttgatgacgt cttcttcacg
    23641 gtaaccaccg agcgttgaat ctgccgttaa aatgatagct ttagcccctg cttctactgc
    23701 tttgttcaaa atgaactcat tgaatctgtc gtctttactc atatataatt ggaaaaactg
    23761 cggtgcgcct ggcgctgcat cggctgcgtc tttgattgtc gtatttgcat aagtgctgat
    23821 ggaaaagatt gatccagcag cagctactcc tttagctgta tcagcttccc ctttttcatg
    23881 tgccaatcct tgggccgctg atggcgcttg gataatcggt gtatctaatt caattccgaa
    23941 aacagatgta tgcaaatcgg ctgagtcgat tccgcgtaat atccgtggca tgattttctt
    24001 tgtattaaaa gaagtcgtat tttctttcat cgtccactca tcttcagaac caccgcgaat
    24061 atagccaaaa gctcctgctt ccatgcgctc ttttacacga ccttctagtt aggcaatgtt
    24121 tacaatatcg attggatgtt cttcattact tgtatgataa gtcatagtag atccttcttt
    24181 catcttcttg attacatata aatcatacta aacttacaaa aagtaagcaa acatttactc
    24241 tcagatactt ttatcgctat tctttccaaa taaatcgact tacaagctga tctacttctt
    24301 ggttttcatg aagcatacta tgagaggcat tttcacctgt cactactttt tcctctattt
    24361 catttccgcg ttgcttcaac aacgcataga ccgctaatgc actgtttaac ggtactgttc
    24421 catcactcaa gtctgtttca tctagttgac cagcaatcaa gaaaaatcga gtggttatag
    24481 gaatattacc gatcatttgc tggtaatctt gatagcggtt agaaacgaca gcaggcccct
    24541 ttgctaactc atctgtcaga gactgcgcac tatcatcgac aaaatcatta aaaggcgcgc
    24601 caattgctgt taatgtctga acttgtggca atgacgtatc ctgtccatac gttcctaaat
    24661 agcgtagtgc actaactcct cccatggaat gccccactat gttgacttta tctatgccgt
    24721 agttttcttt cagatacaaa agacaagttt tgatccattc tgcttgattc cactcgttat
    24781 ttttattatc ttcaaacaat acttggacca tcggattgtc tgcttttcca gacaaatctc
    24841 ctgttgcttg tatttgtcca tctgttccta ctgttaaaac caattctttt actgcttgct
    24901 gatttttttc caaacgccgc aacatccctt taaaggaatt ttctgtcccg ctgtagccgt
    24961 ggaaaaaaag tgttggaata gtggtttttt ctatcacttt actggattct gccgtttttt
    25021 ctttatcaga aacttcagta gtatttatct tcgtcgtctg gcaaccactt aacgcaaaca
    25081 aactgataag gactaaagct gctgcagttg cacgtttgca taaatttttg attggttctg
    25141 tcatacacct cttcctttcc atatagaaaa aagaaagact tgaaaggcca atcatatgat
    25201 cgacatttca agtctcttta ttaatcgatt ttatctactt gatcgaaatc aagttctgta
    25261 ctggtttcac gtccaaacat atcgatgttg actttcaatt tttctttttc tccgtcaact
    25321 tctgttactt gtccttctaa tccagcaaat gcgccttcga tgattttcac gacttcgcca
    25381 acttctactt ctagatcatt ttgtcgcagg ctcataccga tagaacgcaa gatatgattg
    25441 acttcttctg gaagtaatgg tgctggtttg cttccggctc cgtgtgaacc aacgaatcct
    25501 gtcacaccag gtgtgttgcg gacaacatac catgaagcgt ctgtcatgat catttcaaca
    25561 agtacgtatc ctgggaatgt tttatgaacg atttcttttt ctttcccatt tttgacttca
    25621 gtttcttttt cttcaggaac aacgacacgg aaaataaagt cggccatttt catactttgc
    25681 gcacgagatt cgatatttgc ttttactttg ttttcatatc ctgaatatgt atgcaagaca
    25741 taccagtttt tttcaaaaga ttccattgaa ggctcctcat ttcattgtaa aataaaaaaa
    25801 cctcagtaca cccgaagttt ttctttcgct tcattatagc actaaaagta cgttgtggct
    25861 agcgagttat tttaaaatcc agccaaataa gctttggatg gctgtatcca ttacgtaaaa
    25921 catcaatccg aaaataatag aagtttcgat gactactaac gtatctttac ggagttgttt
    25981 tctgcttggc caagttactt gtttgatttc atcttttacg ctttttagaa atttcatgag
    26041 acaacctccc ttgctactgt ttatttcgtt tctttatgta atgtatattg attacaatat
    26101 ttacaaaact tattgatttc caaacgttcg ccgctttttc cttcactgac tgattttgtg
    26161 tagttgcgag acccacaaac ggaacaagcg agtgctgctt ttttctttgc catttacttc
    26221 tgcccccttg ctgagaatca gacttatact tcataacagt atcacgtaac tttttacttg
    26281 tcaatttgtg aaataaacta tctattgttt tggataaaat tatgatatga ttaatttgaa
    26341 attaagctaa aatgagacaa ttggaggtat agcgatgcta ttaaaaacac ttgtatataa
    26401 gaaaaaagat ctgacaaccg ttacagagac tagtacatta gaagaagcat taaaaatttt
    26461 agaaacttcc ggataccgtt gtgtgccgat cttagattct tcaggtaata ttttccgtgg
    26521 aaacatttac aaaatgcata tctatcgtca taaagcaaat ggcggtgata tgacgcttcc
    26581 tgtcactcat cttttgaaaa acgctacaaa gttcatttac ctggatactt ctttcttcaa
    26641 agtattcttt acgatcaaag agcttcctta tattgctgtc cttgacagtg aaaatcattt
    26701 ttatggtatt ttgacacata gtacgttgct taatatgctc tctcaatctt ggagcgtaga
    26761 acaaggaagc tatgtactga caattgcttc cacaggaaag caaggagatt tggctgctat
    26821 tgcaaaaatc atttcaaaac acagcagcat tgccagctgt attactcttg atatcgaaaa
    26881 agacgaatac attcgacgta cattgatcac gctaccggct aacactgaag cgaaagtatg
    26941 tgaaacgatc gtcaaccact tagagaaaaa gaattataaa gtggtagaaa ttgaaaattt
    27001 gcaaaaccaa gaagcataat caaaaagcga aaaaagaaaa cagctaatct gctttctttt
    27061 ttcgcttttt ataattagac acgcttttca aaatcaagcg cgcgatctaa ataatacatc
    27121 aatggtgctg aaacagccat gacgataaac tcacttaatg caagcgtgcc ataggttgtc
    27181 caaaatgctt ggccacctgt tggaactaac atataagcaa tcagtcccat gctgatggta
    27241 aagaagatcg tgttcaatac taaacgcatc tttacatttt tcactttgtt ttgcaataaa
    27301 gctgtcaggc ctaaagaaag caatgtttgg ccgccaccaa aaaccacatc catgatacca
    27361 aaaccaaaga aggcattata gacgatgacc ccgcctaaaa ctccccaaag caatttacgg
    27421 ttaaagacaa ctaaatgatt caaactttct gaaatacgaa actggatcgg ccctgaggat
    27481 acaggcgcaa ctaaaatagt taacgctaga taaagagcca tgatgatgcc atttacaacg
    27541 attacggata accgttgtct gcttttcgtt ttttgcattt tctctcctct tttctcctag
    27601 tttttttaac gcgggatggt tgatgaaccg cggttatgtg aaacaaacaa taattataac
    27661 agacttttca tttatttcaa tccgtcttat ctgcttttaa aaaagcaaaa gcttaaacaa
    27721 tgtaaacgtt tgctaaaaat cgtgggatat ggtataaagt aagtgtaagg ggtcataaga
    27781 attcccagta aagcagacga agtaggatct gcaagaacag aattgttgtg aaacttaaat
    27841 aaataaaaaa ggatgtggga atttgagcaa cctcttacta gagaacggtc tagcaagcag
    27901 ctgaagatct aagtggacat gccgaacctt ggaacttctc cttacgtaag taatggatca
    27961 tgcgcttgct aggctttttt gtgtttttcg gtcttcggac ctttttcatt ttcctagact
    28021 tctgctataa taaccaagaa ttaattttgt aacagatttt tttgaaaaaa caagtttctt
    28081 tcattcaaaa aatagaaaaa gcttgctaat tattggttaa ccagcaaaca ggagtattaa
    28141 aatatcttgt tttattgaat agcaggagga aatcatgaaa ttccattatc atatattgat
    28201 caatccatcg gcaggcagcg gaaatggcta taaagtagca gaaagaattt taccggtatt
    28261 aaaaaataag catattgact atacacttca ttatagtgaa tacaaaggac acgatgctga
    28321 gattgctgaa actttggcaa aagaaacatt gattccttgg atagaagaag aacagaaaac
    28381 aatcgatact ttcccccttt tgatcatcgt aggaggtgac ggcaccttac atcaggtact
    28441 tgatactttc tatcagatgg aagtagaatt cccagttgct tatattccag caggttctgg
    28501 agatgatttt gctcgagggg caggccttcc aaaaaatccc aaaaaaggac ttcagctgat
    28561 tttagcagct caatcaccag aaaaagtcca cataatggct tatgaagaaa aaataagtga
    28621 aaaaaaagga atagctgtta ataatttcgg tataggatta gatgcagcta tcgtccatgc
    28681 gacaaatcat tcttccacaa aaaagcgatt gaacaaatac aacttaggat cattttcgta
    28741 tttattctct attctccgtg ctttattcac tcaaaaagga tttccaattc tggtagatgc
    28801 aggaggaaaa caattaagtt tttcaaacgc atttttatgt acagcaacga agcatccttt
    28861 ttttggcgga ggaatcgcga ttgttccaac tgctgatgcg tctaaaccag tcattgattt
    28921 tgtcgtggtc gaacgaatca atttctttaa aatcttgtgg ctcattcttt tacttataca
    28981 gaaaaaacaa ttgaaatcga aatatttcca tcactacaca acaaacagat tacgtatcgt
    29041 ttcaacaaca ccgcaacatg gtcaagaaga tggagaagaa atggaaaaac gaccttttga
    29101 tatcacaata accacaacta aacagttgtt ttggtgttaa taaaagaggt tttgtcagaa
    29161 gctacatctg gaacaccgtt tattcggaaa aaaggactgt gacacgactt ttgtcacagt
    29221 ccttttttta ctatgcagtt aattctttgc ccaattgggt tgccatcgat agtttctttc
    29281 tttacccaaa ataaattgga tcattgaaaa caccgcagca aaagcaacta agacacaacc
    29341 attgaaccaa aaagattctg caaatgtttg attgtaactg agtgtttcac tgatcaagtt
    29401 attattttga acaaaccaat atgttggtgt gaatgccgct attttgctaa ccatttctgg
    29461 caagtattct gctggtacaa acaccccacc gataaagcaa ctgcctgtga taaaaatatc
    29521 tttgatccca ccgctggttt ctgagttttt taccaatgaa gtgatacaag ccgaaaagct
    29581 aatgatcggc aataaaaaca agaaagtatt taaaatatga taccataccg ttatatccca
    29641 cgaggtgtgg atcactaaca gaaccattag aagtaagaag ccactgaaca acagcagcga
    29701 gtaactaatc aaactaaagg ttactttgcg attcaagttt cgtttggtaa tgggcgcact
    29761 ctggtttcgt ttactgatct ctttgcgatt aaatgctaga ttgatgacgc tgaaaccgct
    29821 aaaaatggat agaaacaacc cataagacaa gaaattaaag gttgtgcccg tcaacagctt
    29881 tttcatccgc tgactatatc ccgcacttag ctcaacttct ccagtaaccg agagtgccat
    29941 ttgtgtctgt tccatagctg catcgattgt tttatcttct tcttgaaaca gaatcagtgt
    30001 ttgcaaaaag gtgttcactt gctgtgtaac aactgtttta gaaaaggcat ctgggcgact
    30061 ctgtatcttg atcgtcggca tctttcctac ttcgacttgt tcggtaaagt ctgcaggcag
    30121 atgtaagata atgtttacac gattaaaata aagagcatca tcgatttctt tttgtgaggt
    30181 tccatccaat gtaacgactg tttccttgtc attcaaatat ttacgaaggc catcgactac
    30241 aggcgactct tcttcactaa tcaaggcgat tttcgcatct tgcagagagg tattttcaac
    30301 atcaatattt tgcgcaaaaa agaatgtggc aacaacagtg ataattacac ctatcagtaa
    30361 attcgattta tttgctttaa gcaatgtcca aatcgtttta taaagcgtca taacgcaccc
    30421 ctctttccat ccaagcattt cctaacgcaa aaaggaaaat aaatccgatc aaccatagaa
    30481 gattggtata aaaactagcc aaatcagtgt agaaataaag tttatacaaa ctttcgctga
    30541 tcaagttgac gagatttaat tgaccaacaa tcggaatatg aagatcaatc caatacttca
    30601 cttgttccga ccccatcata ccagcaaaaa agctcatcgc aatcgtcact gaaatcaaga
    30661 tgctatcctt ttgcaccaga ttcatttttg gtatcaaatt accaaccaat gtacctaaca
    30721 aaatcgcaca caacgacccc agagcggtaa caatcatcaa ccattgccaa cgttcaccaa
    30781 agtctacttg atagataaaa cgaaagacag ctaacataat ataaatctct gtcaaaacaa
    30841 tcgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
    30901 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
    30961 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
    31021 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
    31081 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
    31141 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
    31201 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
    31261 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
    31321 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
    31381 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
    31441 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
    31501 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
    31561 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
    31621 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
    31681 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
    31741 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
    31801 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
    31861 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
    31921 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
    31981 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
    32041 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
    32101 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
    32161 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
    32221 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
    32281 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
    32341 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
    32401 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
    32461 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
    32521 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
    32581 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
    32641 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
    32701 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
    32761 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
    32821 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
    32881 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
    32941 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
    33001 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
    33061 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
    33121 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
    33181 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
    33241 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
    33301 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
    33361 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
    33421 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
    33481 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
    33541 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
    33601 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
    33661 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
    33721 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
    33781 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
    33841 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
    33901 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
    33961 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
    34021 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
    34081 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
    34141 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
    34201 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
    34261 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
    34321 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
    34381 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
    34441 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
    34501 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
    34561 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
    34621 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
    34681 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
    34741 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
    34801 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
    34861 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
    34921 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
    34981 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
    35041 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
    35101 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
    35161 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
    35221 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
    35281 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
    35341 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
    35401 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
    35461 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
    35521 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
    35581 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
    35641 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
    35701 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
    35761 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
    35821 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
    35881 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
    35941 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
    36001 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
    36061 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
    36121 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
    36181 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
    36241 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
    36301 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
    36361 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
    36421 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
    36481 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
    36541 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
    36601 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
    36661 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
    36721 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
    36781 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
    36841 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
    36901 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
    36961 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
    37021 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
    37081 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
    37141 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
    37201 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
    37261 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
    37321 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
    37381 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
    37441 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
    37501 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
    37561 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
    37621 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
    37681 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
    37741 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
    37801 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
    37861 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
    37921 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
    37981 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
    38041 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
    38101 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
    38161 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
    38221 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
    38281 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
    38341 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
    38401 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
    38461 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
    38521 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
    38581 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
    38641 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
    38701 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
    38761 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
    38821 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
    38881 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
    38941 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
    39001 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
    39061 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
    39121 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
    39181 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
    39241 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
    39301 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
    39361 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
    39421 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
    39481 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
    39541 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
    39601 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
    39661 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
    39721 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
    39781 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
    39841 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
    39901 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
    39961 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
    40021 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
    40081 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
    40141 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
    40201 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
    40261 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
    40321 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
    40381 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
    40441 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
    40501 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
    40561 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
    40621 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
    40681 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
    40741 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
    40801 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    40861 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    40921 cggtcgacat acctaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    40981 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    41041 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    41101 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    41161 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    41221 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    41281 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    41341 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    41401 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    41461 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    41521 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    41581 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    41641 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    41701 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    41761 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    41821 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    41881 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    41941 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    42001 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    42061 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    42121 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    42181 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    42241 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    42301 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    42361 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    42421 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    42481 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    42541 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    42601 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    42661 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    42721 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    42781 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    42841 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    42901 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    42961 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    43021 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    43081 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    43141 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    43201 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    43261 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    43321 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    43381 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    43441 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    43501 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    43561 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    43621 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    43681 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    43741 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    43801 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    43861 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    43921 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    43981 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    44041 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    44101 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    44161 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    44221 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    44281 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    44341 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    44401 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    44461 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    44521 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    44581 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    44641 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    44701 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    44761 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    44821 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    44881 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    44941 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    45001 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    45061 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    45121 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    45181 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    45241 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    45301 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    45361 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    45421 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    45481 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    45541 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    45601 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    45661 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    45721 aaaaat
//
LOCUS       DAFFSV010000009        44724 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFFSV010000009 DAFFSV010000000
VERSION     DAFFSV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.009"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            105..497
                     /locus_tag="LR110_000427"
     CDS             105..497
                     /locus_tag="LR110_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL4821432.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            525..1865
                     /gene="glnA"
                     /locus_tag="LR110_000428"
     CDS             525..1865
                     /gene="glnA"
                     /locus_tag="LR110_000428"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBL4821433.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3058..4668
                     /locus_tag="LR110_000429"
     CDS             3058..4668
                     /locus_tag="LR110_000429"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBL4821434.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            4960..5829
                     /locus_tag="LR110_000430"
     CDS             4960..5829
                     /locus_tag="LR110_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBL4821435.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            5994..7265
                     /locus_tag="LR110_000431"
     CDS             5994..7265
                     /locus_tag="LR110_000431"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL4821436.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7262..8557
                     /gene="rho"
                     /locus_tag="LR110_000432"
     CDS             7262..8557
                     /gene="rho"
                     /locus_tag="LR110_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBL4821437.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8656..8919
                     /locus_tag="LR110_000433"
     CDS             8656..8919
                     /locus_tag="LR110_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBL4821438.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9364..9813
                     /locus_tag="LR110_000434"
     CDS             9364..9813
                     /locus_tag="LR110_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4821439.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9870..11288
                     /locus_tag="LR110_000435"
     CDS             9870..11288
                     /locus_tag="LR110_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4821440.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11288..12148
                     /locus_tag="LR110_000436"
     CDS             11288..12148
                     /locus_tag="LR110_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBL4821441.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12322..13095
                     /locus_tag="LR110_000437"
     CDS             12322..13095
                     /locus_tag="LR110_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL4821442.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13242..13607
                     /locus_tag="LR110_000438"
     CDS             13242..13607
                     /locus_tag="LR110_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBL4821443.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13755..14963)
                     /locus_tag="LR110_000439"
     CDS             complement(13755..14963)
                     /locus_tag="LR110_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4821444.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(14982..15260)
                     /locus_tag="LR110_000440"
     CDS             complement(14982..15260)
                     /locus_tag="LR110_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821445.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15414..16109
                     /locus_tag="LR110_000441"
     CDS             15414..16109
                     /locus_tag="LR110_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL4821446.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16551..17348)
                     /locus_tag="LR110_000442"
     CDS             complement(16551..17348)
                     /locus_tag="LR110_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBL4821447.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17345..18529)
                     /locus_tag="LR110_000443"
     CDS             complement(17345..18529)
                     /locus_tag="LR110_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL4821448.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18554..19801)
                     /locus_tag="LR110_000444"
     CDS             complement(18554..19801)
                     /locus_tag="LR110_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL4821449.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20016..21761
                     /gene="dnaX"
                     /locus_tag="LR110_000445"
     CDS             20016..21761
                     /gene="dnaX"
                     /locus_tag="LR110_000445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBL4821450.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21781..22095
                     /locus_tag="LR110_000446"
     CDS             21781..22095
                     /locus_tag="LR110_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBL4821451.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22120..22716
                     /gene="recR"
                     /locus_tag="LR110_000447"
     CDS             22120..22716
                     /gene="recR"
                     /locus_tag="LR110_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBL4821452.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22780..23424
                     /locus_tag="LR110_000448"
     CDS             22780..23424
                     /locus_tag="LR110_000448"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBL4821453.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23439..23768
                     /locus_tag="LR110_000449"
     CDS             23439..23768
                     /locus_tag="LR110_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBL4821454.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23782..24720
                     /gene="holB"
                     /locus_tag="LR110_000450"
     CDS             23782..24720
                     /gene="holB"
                     /locus_tag="LR110_000450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBL4821455.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24756..25580
                     /locus_tag="LR110_000451"
     CDS             24756..25580
                     /locus_tag="LR110_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBL4821456.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25573..25920
                     /locus_tag="LR110_000452"
     CDS             25573..25920
                     /locus_tag="LR110_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBL4821457.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            25989..26861
                     /gene="rsmI"
                     /locus_tag="LR110_000453"
     CDS             25989..26861
                     /gene="rsmI"
                     /locus_tag="LR110_000453"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBL4821458.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            26970..28091
                     /gene="dinB"
                     /locus_tag="LR110_000454"
     CDS             26970..28091
                     /gene="dinB"
                     /locus_tag="LR110_000454"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBL4821459.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28145..28747)
                     /gene="nrdG"
                     /locus_tag="LR110_000455"
     CDS             complement(28145..28747)
                     /gene="nrdG"
                     /locus_tag="LR110_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBL4821460.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29062..31251)
                     /gene="nrdD"
                     /locus_tag="LR110_000456"
     CDS             complement(29062..31251)
                     /gene="nrdD"
                     /locus_tag="LR110_000456"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBL4821461.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31550..32344
                     /locus_tag="LR110_000457"
     CDS             31550..32344
                     /locus_tag="LR110_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4821462.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32334..34376
                     /locus_tag="LR110_000458"
     CDS             32334..34376
                     /locus_tag="LR110_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4821463.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34657..34806
                     /locus_tag="LR110_000459"
     CDS             34657..34806
                     /locus_tag="LR110_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBL4821464.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34817..36331
                     /gene="dltA"
                     /locus_tag="LR110_000460"
     CDS             34817..36331
                     /gene="dltA"
                     /locus_tag="LR110_000460"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBL4821465.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36328..37533
                     /gene="dltB"
                     /locus_tag="LR110_000461"
     CDS             36328..37533
                     /gene="dltB"
                     /locus_tag="LR110_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBL4821466.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37578..37811
                     /gene="dltC"
                     /locus_tag="LR110_000462"
     CDS             37578..37811
                     /gene="dltC"
                     /locus_tag="LR110_000462"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBL4821467.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37814..39082
                     /gene="dltD"
                     /locus_tag="LR110_000463"
     CDS             37814..39082
                     /gene="dltD"
                     /locus_tag="LR110_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBL4821468.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39223..40305
                     /locus_tag="LR110_000464"
     CDS             39223..40305
                     /locus_tag="LR110_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBL4821469.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40305..41108
                     /locus_tag="LR110_000465"
     CDS             40305..41108
                     /locus_tag="LR110_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL4821470.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41178..41918
                     /locus_tag="LR110_000466"
     CDS             41178..41918
                     /locus_tag="LR110_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL4821471.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            41929..42933
                     /locus_tag="LR110_000467"
     CDS             41929..42933
                     /locus_tag="LR110_000467"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBL4821472.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(42978..44675)
                     /locus_tag="LR110_000468"
     CDS             complement(42978..44675)
                     /locus_tag="LR110_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821473.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14362 a         7814 c         9326 g        13222 t
ORIGIN      
        1 taaaaaatag agatctcatg ttagaaaacc taacatatgc agttgacaat atttttttta
       61 attggtacac tttagccaac ttataaaacg aaaggggacg tctgatggga gaaaaggaat
      121 tgcgccgatc tatgtccgta tttccaattg gtacagttat gaagctgact gatttgtctg
      181 ctcggcaaat tcgctattat gaagaacaag atttagttca tccggaacga agcgaaggaa
      241 accgacgtat gtattcgctg aatgatattg atatgttgct tgagattaaa gactatcttt
      301 cagagggatt gaatatggct gggatcaaac gtgtatacga aatggcacag gcgaaaaaac
      361 aagaagcaaa aaacaagaag ccgttgacag atcatgacgt tcgacaaatt tttcgtgatg
      421 agattttggc gcaaggtggg ttaagcaaaa caggtcatta tcagtcgcaa ggattgcaaa
      481 gacaattctt tgactgaata aattaaaaaa ggaagaggga taccatggta aagaaacaga
      541 taacaggtga agaaatcaaa cgaatcattg aggaagaaaa cgtacgattt ttacgattga
      601 tgttcacaga catcttaggt acgatcaaaa atgtagaagt tccagtgagc cagatcgaca
      661 aagtattaga aaataaaatg atgtttgacg gttcttcgat tgaaggattt gttcgaattg
      721 aagaaagcga tatgtatttg tatccagatc tctccacttg gatgatcttc ccgtgggaaa
      781 gtgctcatgg aaaagttgct cgtttaattt gtgatatcta taatccggat gggacaccat
      841 ttgccggcga tcctcgcggg aatttgaaac gagcattgaa agatatgcgc gaccttggtt
      901 ttacctcttt caatttagga cctgaaccag aattcttctt atttaaatta gacgaagacg
      961 gtgggatcac aacgaccttg aatgataaag gtggttattt tgattttgca ccaacagatt
     1021 taggtgaaaa ttgccgccgt gacatcgtgt tggaattaga aagtctaggc tttgaagtgg
     1081 aagcttcaca tcatgaagta gcgccaggac agcatgaaat tgactttaaa tatgcagatg
     1141 tggtagatgc ttgtgataat atccaaacct tcaagctcgt tgtcaaaacg attgcacgca
     1201 agcatggact tcacgcgact ttcatgccga aaccgttatt tgggatcaat ggttctggta
     1261 tgcattgcaa tatgtctttg ttcaatgatg aaggaaatgt tttctatgat aaagacggtg
     1321 aattggaact aagtgaaaca gcttatcatt tcttaggtgg tttgctgaaa catgctcgtg
     1381 cctacacagc cgtttgcaat ccaacagtca attcttataa acgtctcgtt ccaggttatg
     1441 aagcgcctgt ttatgtcgca tggagtggcc gtaatcgttc gccattagta cgtgtaccag
     1501 aatctcgcgg tttatccaca cgtttggaat tgcggtcagt tgatccatct gcaaacccat
     1561 acttggcaat ggcagtattg ttgcaagcag gactagacgg tatccgtaat cagatcacac
     1621 cgccagcagc tgttgaccgt aacatttatg taatggatga ggaagaaaga gaagctgcac
     1681 atatccagga cttaccttca actatccata atgctatcaa agaattgcgt aaggataatg
     1741 taatgattga cgcattagga caacatatct tctcaaattt tgttgaagca aaacgtctcg
     1801 aatgggcggc attcagacaa actgtttccg agtgggaaag agaacaatat ctagaacttt
     1861 attaaaacag gaataccgtg aattagaggc tgtgaaatca gcgttttgac ctgagtatta
     1921 gagaaactaa cggaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg
     1981 tcagctgatt gaacaccgtt tagtggagag gttgtgaaaa agctgccctg caacaagtaa
     2041 taagaatagc caagcaaaaa cagcttttct tgtttttgaa ttggctgaac ttattaccgc
     2101 agatgcaagc ttttgaacac cgtttaccaa gaggttttga aaaagacgtt tagtttcgag
     2161 tattaagaaa aattttgtga aaatggcttt ctacattttt gcataaattt ggcttaatac
     2221 cgagaaactg gcttttgaat accgtttatc cagaggttgt gagcctacca tttaactccg
     2281 aatcacaaga aataatttca aaaacagctt ctcatgtttg atggatattg tgacttgtga
     2341 ccgaggagtt ggcaggagaa caccgtttat ttagaaataa ggaggcacga cttttgtcgc
     2401 atctccttat tttttactta tgtgattcgt taccttcaat gaatccctac aaggcacttt
     2461 tgttgaaaaa aaggtcatct ttgaaaaaat tagtggaatt gttttgtttt tgatattatt
     2521 taatagaaaa gatatgccaa aaatccgaga actgtggact acgtttttta cgtaaaggag
     2581 gtaaataagg gtgaaagaaa aagattgttt ctacgataag ctatataaac tgttgctgca
     2641 agtaggaaaa agtagttctt tgttaggtgg gatattatct atcataatgt ttgttttatt
     2701 tttttatcta ttatttcact taaaattttt cagtaacgat tttctgattt gcttaggttt
     2761 aacgttagct atgtttatat caagcttatt atgtgggtta aatttttttt gcactcataa
     2821 aggacttaag aaataatttc tcaaaggata tcgaaaaaga aaaaattgta agcggatagt
     2881 attcatttga gtacttgact ttcccttagt cagcttgtat tatacacctt gggcaccctt
     2941 ttagagggtc agaagtttac agaaatttcg gctccctatt cttattgaag aatagggagc
     3001 tttgtttatt gtctgagact tttcctgcac ataaattaaa aaggagtttt tgaacttatg
     3061 acaaaatata tttttgtgac cggcggtgtc gtttcatcaa tcggaaaagg gattgttgca
     3121 gcttctttag gacgcttatt gaaaaaccgc ggcttaaaag taactatcca aaaatttgat
     3181 ccatacatca acgtggatcc ggggacgatg agcccatatc agcacggaga agtattcgta
     3241 acagatgatg gtgctgaaac agatttggat ctgggccact acgaacgttt tattgacatc
     3301 aatttgaaca aatactccaa cgtgacgaca ggaaaaatct attctgaagt tttgcgtaaa
     3361 gaacgtaagg gcgaatactt gggagcaact gttcaagtta ttcctcatat tacaaatgag
     3421 atcaaagaaa aaatcatgcg agcagctaaa atgactgatt cagatgtgat cattacagaa
     3481 gtagggggaa cagtagggga tatagaatct ttgccattcc ttgaagcact tcgtcaaatg
     3541 aaagcagatg tcggtacaga taatgttatg tatatccata caacattgat tccttacttg
     3601 aaagctgctg gagaaatgaa aactaaacca acgcagcaca gtgtaaaaga gctacgtggt
     3661 ttaggtatcc aaccgaatat tttggtggta cgtacagagc aacctgtttc tcaaagcgtg
     3721 aaaaacaaat tggcacaatt ttgtgatgtc gaaccagaag cagttatcga gtcgccagat
     3781 gtagatacac tttactccat cccattagtt ttacaagcac aaggaatgga tcaaattgtt
     3841 tgcgaccatt taaaactaga tacaccagaa gcagacatga cagaatggaa agaattagaa
     3901 gaacgtgtct tgaacttgaa gaaaaaagta cggattgcgc ttgtcggtaa atatgtcgag
     3961 ttaccagatg catatatctc agtagttgaa gcattgaaac attctggttt cgcctataat
     4021 tcagacatcg agatcgattg gatcaaagca caagaattaa cgagagaaaa tgtagaagaa
     4081 cgtttgaaag acgcagatgg aatcttggtt cctggtggat ttggtgatcg cggagtagaa
     4141 gggaaaatcg aagcgattcg ttatgctcgc gaaaatgacg ttccgttttt aggtatttgt
     4201 cttggtatgc agatggcttg tgtcgaattt gcacgtaacg tcgttggatt agaagatgcg
     4261 tcatcagctg aaacgaatcc agatacagca aacaatatta ttgatttaat ggctgatcag
     4321 gaaaacatcg agaatttggg tggaacttta cgccttggat tatacccatg taaaatcaaa
     4381 aaaggaacga agacggctga agcatatgat ggagccgatg ttgtgcaaga acgtcaccgt
     4441 caccgctatg agttcaataa caaatatcgt caattgtttg aagaacaagg gctagtcttt
     4501 tcaggtgtct caccagataa ccgtttagta gaaatcgtag agctgccaga gaaaaaattc
     4561 tttgtgggat gccaattcca cccagaattg atttctcgtc caaatcgccc gcaaaaatta
     4621 atcaaagcat ttgtcggagc atcattagca gatcacgaaa caaaataatt tttgaacttg
     4681 atagaaagag aaaagtagca agagaagggg ataagcaatt ttcttgtcac ttttctcttt
     4741 ttctgatttg ttattatttg taggaaaaaa gatagaaagc aaaagacttt tttcgattaa
     4801 agcgctattg gatattttat tttttgcaaa aaacttcttg aaaattttca ttttcattga
     4861 ttttagtaga aaactaccga aatgtttggt aaaatagggt cgttggttaa gaattgtctt
     4921 aacaaattta gttttacaaa acttaggagg aattttatta tgccagtagt atcaggagct
     4981 gaatttttaa aagctgctcg taaaggcggt tacgctgtcg gcggattcaa cacaaacaac
     5041 ttggaatgga ctcaagcaat ccttgaagcg gctgaagcaa aacaagcacc agtacttatc
     5101 caaacatcaa tgggtgcagc taaatacatg ggtggttaca aaatctgtaa agatttaatc
     5161 actgatttag ttgaagcaat gaacatcact gttccagttg ctatccactt agaccatggt
     5221 gattacgaag ctgctttgga atgtatcgaa ttaggctata cttcagtaat gttcgatggt
     5281 tcacatctac catttgaaga aaacttgaaa ttagctaaag acgttgttga aaaagcacat
     5341 gcaaaaggta tctcagttga atgtgaagtt ggttcaatcg gcggagaaga agacggaatc
     5401 atcggtaacg gcgaattagc tgatattgaa gaatgcaaac aaatggttgc tacaggtatc
     5461 gactacttag cttgcggtat cggtaacatc catggtcaat atccagaaaa ctggaaagga
     5521 ttagcttttg accacttaca agcaattgct gatgctgtag gtccagacat gcctttagta
     5581 ttacatggcg gatcaggtat tcctcaagat caaatcgaaa aagctatctc aatgggtatc
     5641 tcaaaagtaa atgttaacac tgaattccaa ttatcatttg ctgcagctac tcgtaaatat
     5701 atcgaagctg gtaaagataa agaaggtaaa ggattcgacc ctcgtaaatt attagctcca
     5761 ggtaaagcag caatcgttga agatgctaaa tctcatatcg actggttcgg ttcagctaac
     5821 aaagcgtaag catccacata acagatacga tatataggaa tggagaacga cttttgaagt
     5881 cgctctccat tcttttttac ttaaaacggc agaccatgct cgtttctttt ttttatctat
     5941 gataaaatac aagcatatcc atgaaaaaag attaataaac aaggtggaaa cagatgaaaa
     6001 aaatcgtaat caaaggaaac cgacctttag ctggtgaagt aacgatcagc ggtgcaaaaa
     6061 atagtgcagt cgcattgatt ccagctgcaa tcttagctga ttccccagtg atcttggaag
     6121 gtgtgccgga catacaagat gttcattcat taattgaaat tttagagatc atgggcgcaa
     6181 ctgtccattt ttccaacaac atactagaaa tcgatcctcg agaagtcgta tcgattccga
     6241 tgccgcacgg aaaaatcaat agtttgcgtg cttcttatta ttttatggga acactgcttg
     6301 gtaaatttgg cgaagcggta gtaggcctgc caggtggctg ttatttaggc cctagaccga
     6361 ttgacctaca cgtcaaagga tttgaagctc taggtgctga agtgacaaat gaacatggtg
     6421 ccatgtattt acggacagaa aataaaggat tgcgtggcaa tcggattttc atggacgtgg
     6481 tttcagtcgg agcaacgatc aatgtgatgt tagctgctgt aaaagcaaaa ggacagacag
     6541 taattgaaaa cgctgcacga gaaccggaaa tcatagatgt ggcaacttta ctgaataata
     6601 tgggggcaaa agttcgagga gcgggtacag atgtgatccg tatcgaagga gtcgaaacat
     6661 tacatggctg tcggcatttc atgattcctg accgcatcga agcaggaacc tatctagcat
     6721 tagcagctgc agtaggaaat ggtatcaaag tgaaaaatgt gatctttgaa catttggaaa
     6781 gctttatagc aaaattgcag gaaattggtg tccatatgaa aatcagcgaa gatgaaatcg
     6841 aagtttaccc ctcgaaagaa ttaaaacccg caaacatcat gacctatcct tatccaggat
     6901 ttgccacaga tctgcagcag ccacttactg cgttactatt gatgactaca ggcacttcag
     6961 aaatcatcga tacgatctat gcgaaacgtg tgaaccatgt gccggaacta gcacgaatgg
     7021 gcgctgatat taccgtggaa ggaaatatga tcatcgtgaa tggtccaaat aaacttcacg
     7081 gcacagaagt cgtggcttct gatcttcggg caggtgcttg tttagtgatt gctggactat
     7141 tggcagaagg gacaacgacg atttacaatg tagattatat cttacgcggt tatgatcata
     7201 ttatcgagaa actaacagcc ttaggggcat caattgaaat gatagaggaa gtgggagctg
     7261 aatgagcgat tatttgacaa tggcagaact agagaacaaa acgttaaaag aagtttatag
     7321 ttatgccaaa gaatttaaaa ttccttatta cagtaagatg aataaaaagg aattatctct
     7381 agctgtgatt cgtgcacaag cagagaaaca aggattttat tatatggaag gaatattgga
     7441 tatcgttggt caagatggtt acggtttttt acggccaatc aattatggac caagtgcaga
     7501 agatatctat atttctgcct cacaaatcag acgattcagt ttgagaaatg gggacaaagt
     7561 ggcaggaaaa gcccgtccac ctaaagaatc ggaacgttat tatggtttga tgcacgtgga
     7621 aagcgttaat ggaaaagatc cggaagaagc aaaagaacgt ccgcattttc cagccttaac
     7681 acctctttat ccgcaaagac agcttcaact ggaaacaaca ccgcaaagac tatctacccg
     7741 aatgatcgat atcttttctc caatcggttt tggtcagcgt ggattgatcg tcgcaccgcc
     7801 aaaagcaggg aaaacttctg tattaaaaga aatcgcaaat ggtatcaccg aaaactatcc
     7861 ggaagtagaa ttaatcgttc tactgattga tgaacgaccg gaagaagtaa cggatctaga
     7921 acgaagcgtg aaaggcgacg tggtatcttc tacatttgat ctgcagccgc aaaaccatac
     7981 tcgagtttct gaattagtat tggaacgcgc aatgcgttta gtcgaagata aacgagatgt
     8041 cgttatcttg atggatagta ttactagact tgctcgggcc tataacttag tcgttccacc
     8101 aagcggacgt acattgagtg gcgggattga cccagccgca ttgtataaac ctaagaaatt
     8161 tttcggggca gcaagaaaca tcgaagaagg tggcagtttg acgattttag cgaccgcttt
     8221 agtcgataca ggaagccgta tggatgacgt gatctatgaa gaattcaaag gaactggaaa
     8281 tctagaatta caactttcac gtgaattatc tgaacgtcgg atcttcccag ccatcgatat
     8341 caaaaaatca ggtacccgaa aagaagaatt attgatgcca agtgaagaat tagaagaaat
     8401 ttggaaactt cgaaaaaata tgactggaga ttcattagaa tatacggaac aactgatccg
     8461 ctttttgcgt aaaacaaaaa acaatcagca atttttcaaa gaatttcaag atgtctcctt
     8521 tggtaaaaaa aatccgacca gaaatctgaa aagataattg caaccttgac tgaaacatgt
     8581 taagatgtta atgttgtaaa tcgacaaatc aactctggtt cagcaaatgg agcagggcaa
     8641 aggagcgaaa aaatcatgaa agaaaacatc catccagatt accacccagt agtattcatg
     8701 gattcaacaa ctggtttcaa attcttgtca ggttcaacaa aaacttcaca agaaacagtt
     8761 gaatgggaag acggtaatac atacccagtc atccgtgtcg aagtaacttc tgactcacat
     8821 ccattctaca ctggacgtca aaaattcaca caagcagacg gacgtgtgga ccgtttcaac
     8881 aaaaaatacg gtctcaaaga cgcaaacgct gcggaataat aaaaacgctg ataactcaac
     8941 gtttagcaga ctatctgata gggatagtct gtttttttat gttttttgcc aatttaaaac
     9001 ttcgtttgat gctgaaaaag tcgtaggatc ataatgcaca aaacaatgaa aaactagtat
     9061 atagttttaa ttatccactt gataaaaaca ccttatatta acgaaatgaa gaggggttat
     9121 ttcgaagtaa aaaaccattt acctaaaatt agaagctaag ctttcctttc aaggaaggct
     9181 ttttgttttg ttatagactt gcgattgttt gtgagctaga tcgttcattt taatgactta
     9241 tacacgcgga tgatgggtct ttttcctctg ttcattcata atattgaacg gtacagctag
     9301 cgatgctata ctgacctcag ttccaagaaa acgttaacat tttttgtggg ggagaggttt
     9361 tttatgaggt ttgatcaatt gatcgcagaa gaactgattg ttttcgatcc atcgatagat
     9421 tcaaaaggat cattatttga gcgaataggg gatgtgttga aaaaaacaaa taaggtaaga
     9481 aatgttcaaa aagtcgttaa ggcactattc aaacgagagg aggagatctc cacaggaata
     9541 gaagatggat ttgggattcc ccatgcaaaa agcaaatatg tgacaaaacc agcactgtgt
     9601 ttttttcata cagccaaatt aacggactat agcgggatag atgaaatgcc aattgaatat
     9661 atttttgcca tcgtagttcc tgaaaaatca gcagatatgc atctagagat tctcagcaca
     9721 ctctccagac gattgatgaa tgcagatttt agaaagggaa taaaagaagc cactacgcca
     9781 gcagatatca tcagacagat tagtcaagaa tgaaaatagt agagtctttg tccgagtatc
     9841 gatagtgaag aaaaaggagg aaaataatca tgaaaatcgt tggtgtaact gcttgcccga
     9901 ctggaattgc tcatacttat atgtcggcag aaaaattaga agtaacagca agaaatctaa
     9961 accatcaagc aaaattcgaa acacaaggtg taaaaacaga aaatcagtta tctgaacaag
    10021 aaatcaaaga ggcagatgca atcattcttg cagtagataa agaaatcgaa ttggaccgat
    10081 ttgccgggaa aaaagtaaag cgtgtctcta caagccgagc aatcaaagaa ccgcaagtag
    10141 taatagacga agcattgaga gatattggcg tattcgttgt gtctaatgaa aaagaaccag
    10201 ctgcaaatga gaaaaagccg acaatctata atcatttcat gaatggtgtg aattacatgc
    10261 ttccatttgt gatcgcaggc ggaatcatca tcgcactgag ttttgccttt gggattacag
    10321 cggctgatcc caactcagca gactataatg tgttagcagc tgcattttca aggatcggag
    10381 gagatacagc gtttgccatg atggttcctg cgttaggcgc ggggattgct acatcgattg
    10441 ctggaaaagc aggttttgcc ccaggaattg ttgccggatt actggcctct acaggtggtt
    10501 caggttttct tggcggaatg attggtggcg tgctagcagg gtatatctgt gattttctgg
    10561 caaataaaat tagcgtaaaa aaagaatttt ctgctattta tcagctgatt gtcgtcccct
    10621 ttttatcgat cttaattatt gggcttttga tggtatttgt gattgaacaa ccgatcacat
    10681 gggtgttgga tgctttgaca aattggctga atagcttggg aaatacatcc gggctattgt
    10741 tcggattact tatcgggatc atgatggcag ctgatatggg tgggccgatc aataaatcga
    10801 cttcgacttt ctcgatcggt ttgatgtcag ctggagtaaa tgcaccgatt gctgcttgta
    10861 tggctgcagg aatgacacct cccctaggta ttgctttagc cactttatta ttcaaacata
    10921 aatttaccaa agaagaacgg acttcaggtc aatcatgctg gattttaggc gcttcgtata
    10981 ttacagaagg tgctataccg tttgcggtat ctgacccgat ccgcgtgata cctagtttga
    11041 tgacaggttc agcagtcgct gcagggattt ccatggcagt aggagttact tcaatggcac
    11101 ctcatggagg gatttgggtg atgctaattc caaatgttat gaacaattta cctatgtatg
    11161 tcttggcaat tgcatcaggt acagtcacta cagcctttat gcttggacta ttgaaaaaaa
    11221 cagtgaaaga gaaaaaacaa gacaagtgga cgaaaaatga aaatctaaca aatgaggtgg
    11281 aagcgtaatg ttatatacaa tgaaagagtt attgaaagtg gctaaagaga atcaatttgc
    11341 agtaccagcc ttcaatattt gcagctatga tatgatgaaa gcaatcatcg aagaagcaga
    11401 gagattagaa gcaccagtaa tcttagaaat tcatcctgat gaaatcgcct atttaagtga
    11461 tgagtttgtt gcggcagtaa gagcctatgc ttatacaagt aaactgccga tcgttatcca
    11521 cttggatcat ggcggtacga tagaagatgt cttacgggcg attcgaaacg gctattcttc
    11581 cgtgatgatc gatgcgtctt tgaaatcatt cgacgacaat gtacagatta cgaaaaaagt
    11641 ggttgcgtta gctcatgcgg taaatgtctc agtggaggct gagcttggaa cgattgggaa
    11701 taatggttct gctgaaggag gtgctgaaac aatcatttat acagatcctg atcaagcgaa
    11761 gatttttgtg aaagaaacag gaatcgatac acttgctgta gcaattggaa cagcgcatgg
    11821 cttgtatccg aaaaacagaa ctccaaaatt aaatatcgac ttactgaaag aattgaacca
    11881 acaaatcgat cttccatttg ttttacatgg cggttcaggt aatccggacg cagaagtcag
    11941 cgaagcagta aaatacggag ttgccaaagt gaatttgagc tcagatctta aaagcgtctt
    12001 tttcaatgct ttgagagaaa tattgagtga aaatccatcg atgtacgagc cgaatgcaat
    12061 ttatccagca gctaataaaa aggtacaaga agtggtcaaa cataaaatgg aaattttgaa
    12121 cacagtaggc aaggctagtt tatactaaaa ataaaaatgt gtggtcaaga gggtatgaca
    12181 atagtcttgt cataccctct taaccacaca cgatgttcag aagtagtttt ttcgaaaaat
    12241 ttcttcttat ttctcgaagc caatcacttc tgtcacaacc tcttttttcc tgttactata
    12301 taaagtaaat ggggtgattt tatgttgaat gaagtggaga atagacagca ggagattgtg
    12361 aattatctaa aaaacaacca atttgcaaga gtcaccgatt tgatcgagtt agtaaattat
    12421 agcgaagcca cgattaaacg tgaccttgtt tttctggaaa aaagtggatt gatccgtaga
    12481 gttcgcggtg gcgccatgct tattgacaat cgaaaaattg acgtgcctta cctgatgaag
    12541 atcaccaaat tagaagaaga aacagaaaaa cgtaagattg cagatacagc ttcttctttg
    12601 attcgagatg atatggtctt attcttggat tccagtacaa cctctttaca cttagtcaga
    12661 aacctagcaa gatttgatgg tttgcagatc attacgaatg gcatcgtcac agctgctatg
    12721 ttgagtgaat atacaacggc aaaagtatct gtattgggag gggcaattgt tccgaaacga
    12781 gcaacaatca acggttcaaa agcctataac gatgcattgt catattatgc ggatttagcg
    12841 ataatgagtt gtcgcgggct agatttcagc caaggagtaa cagaaactca tgaaggagag
    12901 gcaatggtca aaaaagcttt tagaaaacaa tcagaccggc ttgccgtttt agttacacag
    12961 caaaaaattg cacataaatt catgcatcaa gccattgctt ttcatgaaat cgattatctg
    13021 gtgacaagta aagaactgac aaaagaacag tatgcgaaag taaaggaaca ccatatcgaa
    13081 tgtctgtacg gataaattat tacgtgaatg ttctaagtgg cttcctcatt tttagatagg
    13141 accttatctt tgctcgcttc tctcagccga tggtataata atgaaaaggt tatcttgtta
    13201 gataatctta cttttttatc caggagaaag gagtgcagaa aatgaaacat gtgaaggcgt
    13261 tggtagtgaa ggcaatcatg atctgggcga ttctgtgggt cgtgctgaca ggtttatacg
    13321 gtgttagctt tatggattca acgattgttg gggtaattat tgtagtcatg atctatgtgc
    13381 taggggatct gttgatctta cgtaaagtag ggaacatcgc agcgacgatc gcagatgctg
    13441 gttcagctgc agttatcttg tggctctatc tatattttat gaatgataca gtcgatattt
    13501 ggatgaaagt attgattcct gctctattga tcggtgtggc agaatggttc ttccacaagt
    13561 ggttgctaag tcaaggcatc gtaccagatg aacggaaaat ggaataaatc aacagttaaa
    13621 ctattcttat cgataaatga aagctttaaa aataaataga tgtttaatta agaataatca
    13681 taaactattt ttactcaaaa aaccgctcag attattttca gatctgaacg gtttttttta
    13741 tctattcatc aagatcaaat ttcatttttc aatgattcaa tcaattcgcc ctctgcttca
    13801 tgactatgtt ttcggccatt aacaagatag agtgctgtac atatcaatgg aatgactccg
    13861 gcaatcacat acaatcctct aaatgaagta agagatttta gcatccccat caaataagga
    13921 ccaacgccta agcctaggtc taaaccaata aagtaggtag ataaagcaat actgattcga
    13981 tgttcgcgga cgatttttaa acaaacagcc tgtccatttg acataaacgt accataacct
    14041 aggccgacta atgctccaga gataagcaat gtcacactat tcgtcgttac actcagtaaa
    14101 aataatcctg ctgttaagaa taaatagcta ggatacatca cgtattgttc tcctttgaca
    14161 tcgaaaatcc ttccagtcaa tggacgtgta aatgtgatga ccaaagcata tacgacgaag
    14221 aaaaatgacc cagcagcaac taagtggatt acttttgtat aagaagataa aaatgataaa
    14281 acacttgaat aagaaagacc catcaaaaag gcaattcctg taatgaaaag tgctttcttt
    14341 tcaataaaac tattgatatt ccatgagcga agagatattt tttgttcttc agataatgga
    14401 atatttttta caggaaagac aaggcaggca atcgtaacaa agaagaccag tacaattgaa
    14461 aaccagataa tgaaacgaaa atcagtcata ttcagcaaaa tcattcctaa aaatggccca
    14521 acagcggcag ctaaactagt acttaatcca taataattga tgccttcccc ttttttagat
    14581 gcagggatat atgcagtaac gatagcattt gtagctgtag aaactgtacc gtagccgaat
    14641 ccattcaaga atcgaactaa atacaatgcc ccgatagttg gcgtataaag atatgctaat
    14701 gttgaaagga gatagaagat cgcgccgcct cttaaggtaa acttccgacc aaatagttct
    14761 aactgtttcc ccatgaataa gcgtgccagc aatgttccga tgatatagat cccagaagca
    14821 aaacctgctt ggctcaagct ggcgtgtaag gtgtcttgcg caatgactgc catgatgacc
    14881 atcaacagat aataaatcag gtagacaata aaattgatca atgtgattcc aataaaatat
    14941 ttattaaata atttcttttc catgtatgtt atacattccc cttaagctga atatgctttt
    15001 aatttttgta tttgaacttc tacatcgtga taattggatg gatagcgatc aacaagttgg
    15061 cgaataaata aatctcttgt agacataaac agatttaaaa attcttcgca ttcgtttgtt
    15121 ggattctcat ccactgcttt gatcaattcg atcgagtttt gatacgcatc atttaaaaag
    15181 actaaagaag ctttcatatc aaatcttgtt tgatatgctg tttctaatcg attaatgatt
    15241 gtgttcatcc agtaattcat gttttttccc tctattctta aaaaatttta cttctgcagt
    15301 ataacgaata aaaaaaagga aaaaaatcgc ataagtgatg gaaaaaggta cttaacaaat
    15361 gttaatatca ttagtaaaat acctttggac tttaaaaagt tttaaagtac tgtatgatct
    15421 ttttagatga aagtttggat gggggagcat cattggatag tagagtctta aaggaatatt
    15481 tggataaaaa tgattttccg attatacgca aaggatatca taaatatttg acattcgaag
    15541 ggatggaaga ttcccatacg tatatcttga aagagggaac ggtaaaagca agtgttattt
    15601 caaatgatgg gagagaattt aaccttcgct atatcaaaga tcttgaaatc gtctctcttt
    15661 taaaagacga atattcacaa ttcatcgatt cgccgtataa tattcgagtt gaatcagaat
    15721 atgcagagtt ttatcgaatc aatcgtgtgc gcttttggca aaatatcaac caagacccaa
    15781 agctacaaca ctatgtaaaa gagtattacc gctatcgtct gctttattcg atgaaaaaaa
    15841 tgcagcaaat gctattaaac ggaaagctgg gagctgtttg tacgcaactt tacgaattat
    15901 atgatttatt cggtgtgcgg acagaagaag ggatgctgat cgatttttcg atcacgaatg
    15961 aagaaatcgc caaattttgt ggcatctcaa ctgctagcag tgtaaacaga attttaaaac
    16021 aactgaaaaa agatggtatt atcaccgtta aagaccatca tatcgttatt ttgaatatgg
    16081 acttgttaga agacaatatt atcttttgat gaacaaagag gttgtgaaat cagcgttttg
    16141 acctgagtat tagagaaaca aacggaaaaa tagttcctca tattttgccg ttttttgagc
    16201 taataccgag ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctgccgttta
    16261 actctgagtc acaaggaaca atttcaaaaa acagcttttc atgtttgatg aatattgtga
    16321 cttgtgaccg aggagttggt aggagaacac cgtttaccaa gaggttgtga aaaagctgtt
    16381 ctgcatcaag taataagaag agccaagtaa aaacagtctc ttatgttttt aacttggctg
    16441 gacttatcac cgtagatgta agcttttgaa cgccgtttat ttgcaacaaa aggggctgtg
    16501 acacgatttt tgtcacagcc ctttttttgc gaacattcat ttagcaattt ttagaaactt
    16561 ctgccgccac cgccaaaagt cccgccaccg ctagaatggg tagtacttcc gccaccgccg
    16621 cttccaggag gtgggctttt ggggattctt cttgtcgtga caaaagaatt tgtcagacga
    16681 tcggtttttt cagttaattt gatactggat ttttctcgga atggatattt gtagatgcct
    16741 gatttcaatt gataacgcga aacagtcaca aaataaaaga caagactaag gataagagcc
    16801 aaaacaacag caatgatgat ttctactgtc gtcaaaacct tatagcgtgt aattttgcca
    16861 gtttcttcgt ctacacgata atgtcctcct ggaactccgt cttccacata tgttgctgat
    16921 ttagacaggt aagcttttgc agcattgtaa taggcagcat ttgtcatctg atcataaaca
    16981 tcatctaaga tcgaatcgat tctgctatca gtcaaataat cgatcatatt accagaagtc
    17041 gaaatgtaga tttcacgctg tcccatatct aatagcaata ctgagccatt ttgatcattt
    17101 ccgactgcgt ctcttaagta attatcagca aagtctcttg gttcttctga attagatgta
    17161 gtcgttacga tcaagactcg tcctttgatt ttttcattca gcttttctgc ctgttgttcc
    17221 aaagattgga tttgatctgt tgtgaataac ccagcctcat catttaccgt agctgtatct
    17281 gcatgagtag ccaccggcca gccaacactt actagcaaaa gaaagaataa aaaaaatttt
    17341 ttcatcatat caagtaacct ccgatcagaa agaggatcgc tagcccagcg aaaatcccaa
    17401 gagcggtcag tcccaatttt ttctggctga tcggcaggac accgctgacc tttcctgttt
    17461 gtccattcat tgcgtagtag tagacttttt tacttgagtc attgcttcga taagtaacaa
    17521 gccataccgg cagtaatacg taattattta cttcgtttgt gattgaagca gaagttcgca
    17581 caccggtcaa tgttgtatat ccatttgcgg tatcttttaa cagcttctct gaatagtctt
    17641 ttaattcaga ttgaatctgt gatttgattg cctcatattc gatatctctt ttttctgctt
    17701 ggaacccggc taaatactgg cttttaaaat caattgcttt atctaacggg aaaggttgca
    17761 cgccttcgac catcttttgt tgcgtatttt ttgataaggc attttttact agttctttaa
    17821 aagaaagctt gcctgctctt gaaacagcaa attgcttcgt ttctgtgtat tcgatgtcac
    17881 ctatccgcca tatgcggata actgtaccat tcgcctgcat ggcaccatct acttcagcat
    17941 ctgtagccca ataagggaaa tacacacctg tcaacttatc gatttgatcc ttattgaaaa
    18001 aagctttggg aacaaaccat tttttcttgg tccaagcgag aaatctgttg attgcttctt
    18061 ctttctcgac agcaaaagga agtactttat ttggcaagaa ttctccgctt aaacgtcccg
    18121 atagtacgac gggattatgg cagtagtaac aataagtcgc agcagttgtc gcatcagtca
    18181 cgatctctgc accacagttg ggacaaagaa aaagatctat cgctgcggta ttatctgcag
    18241 attcgtccgc tgtatctgaa aaaccgcctg cctcgtcaac cgctttgcgc tcggcttcat
    18301 tcatatcggc aagctgttct tgggctgtaa aagtaaaatc ttcggtaggt gaaggttctt
    18361 ctttgctttc ttcgccagcc atacgcgctt ctttttgctt ttgctcatat tggctgactt
    18421 cttcttctgt atagatattc aggcagtatt cgcaatgaaa tttttggtct ttgggatcaa
    18481 aagtcaacgg accgccgcaa ttaggacatt tatgtgtaag aacatccatc agctgcacac
    18541 ctttctaaat aaattaatta acggggattc ctttaaacgg tttaccacag tttggacaga
    18601 atttagggat gccgttggaa agatccacga tctcattgca ttcactgcat cgcatttgta
    18661 gcttaggctg aacgttttct acaggtttgg cagctccgca gttggaacag aattttccgg
    18721 aattttctgt cccgcaaact ggacatgtcc aagtatccgc atttccttgt gcagtttgtt
    18781 gattttgttt ttctgcttgt tgtttgattt gttcttggtt attttgagct gcttgagaaa
    18841 gataagaacc atttgcattc attcccatac ctacacccat aaagccggtc attgctcctg
    18901 cttcattttt tccggctgct tccataccac gtgcaatgga accttgcacg tatccttcac
    18961 gtacgctagg atcacctagc atcgcccctt cgttacgcat attgatcagt ttcacagaat
    19021 catcggtgta agaaatgctg gctacagcca cagaaacaat ctccatccca cgtaattcac
    19081 gccaagaatc atccaatgca gtatccatgt atttgcttaa ttccaaactc ttagatggta
    19141 cataagaaat ccgttgacca tctgcagaca tctgattgat tgctgattgt agcgctgtta
    19201 ggaattcagc taaatactgc tcattgatat cattgatttc cacttgtgtt ttgtttttag
    19261 ggattgcgtt tgtataaaat aaaatcgggt caacgacatg aatagaataa gtgccatgtg
    19321 cccgtaagaa taattctgcg ttatagaaat tgtcaaagta gttcaacggc gcgcttgtac
    19381 cgaatttgat tccctttatt tcttggagat tgatataaaa aacttgttgt ttctgcggtg
    19441 tgacgccgcc aaatttgaat cggtcaaaag tttcagcaat tgcttctttc agtgttccat
    19501 tgaacatgga aggagctgcg tcatttttaa tggtgtaata accttcctca gcggtataat
    19561 cgatgatttt tccaccatcg accaaaagca tcatcatgtt tggatagaca tggacgactg
    19621 ttccgtctgt taaaacatct tctgttcctt tacgattgga gccacgtttg tcatctttgc
    19681 ggactttgac accttttgtc atgaccgttg tatcactcat attgtctggt tcgatgactt
    19741 ctagccattg atctgccaga ccgccgccaa ccatacttgt tgctgctttg ataagaccca
    19801 taaaaaattc ctcctctgat tcagctagtg ccaaacaatt attgtttatt tattatacca
    19861 taggaagatg tgaaacttct cggactagag acggaattga aatggaaaat tgcgtattgt
    19921 ttaaatacct gaaaaagccg agaaggatag tagttttttt acctaatatc acgtataata
    19981 agagatagtg aattttttaa aaaaggggga tccttatggc atatcaagca ctttatcgag
    20041 tctggcgttc gcagcgtttc gaagacatcg ttggacaaaa agctgtgaca cagacactaa
    20101 aaaatgctat tgtttcccac aaaacctcac atgcctactt gtttacagga cccagaggaa
    20161 ccggaaagac gagtgcagca aagattttcg caaaagcaat caactgtccg aacagtaaag
    20221 acggtgagcc atgcaacgaa tgcgaaatgt gtcgatcaat tactgctggt acgcaagaag
    20281 atgtgatcga aatcgatgct gccagcaata acggagtcga agaaatccga tttatccgtg
    20341 atcgggcaaa ttatgcgcct acaaaggcag cgtacaaagt gtacattatt gatgaggtac
    20401 atatgctttc aacaggagct ttcaatgcct tgttgaaaac attggaagag ccaaaagaaa
    20461 gtgtcatatt catcttagca acgaccgaac ctcacaaaat accagctacg atcatttcac
    20521 ggactcagcg atttgatttt aaacggatca atacggcaga tattgtcgaa catcttgaat
    20581 ttatcttgaa agaatcgact atcagctatg aaccagctgc attgaatgtg attgcccgtt
    20641 ctgcagaagg cgggatgcgg gatgcattga gtattcttga tcaggcaatc tcgtttagtg
    20701 aaggaacaat tactttagat gatgccatgc aagtgacagg tagcttaact gacgaaatga
    20761 tggacagtta cttgagtgct tgcgtgaaaa aagaagtacc agaagcattg gaaacattga
    20821 accaaattct cgcagccggc aaagaatcac gtcgctttct agaggatatg ttacaatact
    20881 gccgtgactt gctgatgtat cagcaagcac ctaacctagt tgaggaacaa tcgagtacga
    20941 tcagaagtac atttacagaa ttggcacaaa ctgtttcagc ggatcagctg ttttcaatga
    21001 taaagatttt gagcgatact caaaatgaaa ttcgttttac caatagtgcc gctatttatt
    21061 tggaagtcgc tacagtcaaa cttgcacagc taccgtctgc ttctgttcct tcagcagatg
    21121 tagcatttgc agatgatcag atagtaaaac agctacaaca agagattaag gaactaagaa
    21181 acgaaatcaa ggaactacgc agtaatacag gaattgagaa tacaaaagta cagccggtac
    21241 agcaaacaaa tcataaaaaa cagacaagta cctatcgtgt accgacggaa cgagtatata
    21301 aagttctgcg ggaagcgaca agacagcatc tgaaccaagt aaaagaagta tgggaagact
    21361 tgctgatgag tctatctgtc actcagcgag caatgcttaa agcaagcgaa ccagttgcag
    21421 ctggacctac aggactagtg attgcgtttg attatgagat tgtttgtcaa agagcagcaa
    21481 atgacgaaga attgcagctt tcggtacaca acaacttaag ccgaatgatc aaagattatg
    21541 cgcctgatcc tgtttatatt acaagagaaa gctggccaga acttcgaaga aattatttga
    21601 cgcaggcaca agaagaggga acagaaaact cttttgcttc tgaagactca gaaggagaaa
    21661 tcgagttgct tcctcaagaa caagaagtac cagaagaaac acttgtagta aaagcagagg
    21721 aactgtttgg ctccgaagct gttacagtgg tagatgatta ataaaaagga gtagataatt
    21781 atgatgcgtg gaatgggcaa tatgcaaggc atgatgaaac aagtacaaaa aatgcaaaaa
    21841 gaaatggcag aagcacaaga gctgctaaat aaaacagagt ttacaggtaa agccaccaat
    21901 gaattggtca aagcggtttt tacaggcgat cgtcgcatga aagatatcca aatcaatgaa
    21961 gaagtcgttg atcctgatga tatcgagatg cttcaggatc ttgtcgtaat ggcagtcaat
    22021 gatgcgatcg cacaaattga tgtggaaact gagaaaaaat tagggaaata cgcaaaaggt
    22081 ttgccaggat tttagtttta aactgaagga gtaatgaata tgcaatatcc agaaccaatt
    22141 gcgaaactga tcgacagcta catgaaactt ccaggaatcg gtcagaaaac agcgacaaga
    22201 ctcgcctttt atacgatcga tatgaaagat gaagtagtaa atgaatttgc taaatctttg
    22261 ttaagtgtaa aacgcgattt gcatttttgt agtatctgcg gaaacatcac agaagaagat
    22321 ccctgcgaaa tatgtagaaa tccttcacgt gatcgaagcg ttatcctagt ggtcgaagag
    22381 ccgaaagatg taatggcaat ggaaaaaatg cgagaatatc acggacttta tcatgttctc
    22441 catggtgtgt tatcaccgat ggaagggaca ggtccagaag acatcaatat ttcttctctg
    22501 ttgcagcggc tgcatgatgc agatgtaaaa gaagtgatta tcgcaacaaa tgcaacaaca
    22561 gagggagaag caacagctat gtatctctca cgtctgatca aacccgcagg tatcaaagtg
    22621 actcgtctag cacatggtct gtctgtaggc tctgatatcg agtatgcaga tgaagttact
    22681 ttattgaaag ctgtcgaagg aagacgtgag ttataaattt ctaattaagt gttttttcag
    22741 atacaatata aaagcagcac aagagatggg ggcaacaatg tgaacggact atttattaca
    22801 atcgaggggc ctgatggtgc aggaaaaacc agcatcttga atgaacttta ccctcttttg
    22861 aaagaagtag caaaaacaga aatcattcaa acaagagaac caggaggcat tccgattgct
    22921 gaagagatcc gagcagtgat ccttgatcca aaaaatgatc ggatggatga acgtacggaa
    22981 gcactgcttt atgcagcagc aagaagacaa catctagtcg aaaaggttct gcctgctctg
    23041 gcaaaaggga aaatcgtttt atgcgataga tttgtcgaca gttctcttgc ttatcaaggt
    23101 gcaggccgac gcatcggcgt taccgagatt gctcggttga atgagtttgc tacagaaggt
    23161 actacgccgg attttaccct ttatcttgac gtagattcag acaccggatt aagaagaatc
    23221 aagaaaaaca gacaaaatca aatcgaccgt ttagattcgg aaggtttgga attccaccag
    23281 cgtgtacgtc atgcttactt aaagctggca gaagagaatc cggaaagaat ccacaaagtt
    23341 gatgcaagaa aaagctttga agaagtgctg caaacaagtt atcatacaat tattgaacag
    23401 tatccgcaat tttttgaaaa ctaggaaggt gaacgtttat gaaaattatt ttagcgatta
    23461 tccaagacaa agacagcaac cgtttatcaa atgaattgat tgatgcaaat atccgtgcca
    23521 caaaactatc atcgacaggc ggcttcctta aggctggaaa cagtacgttt atcgtgggta
    23581 ttgaagatga ccgtgtagaa gaagcattgg aaatcatcaa aaaaacgtgt gaatcaagaa
    23641 agcaatttgt atctacacct gttacactgg atatttcgat ggatgggggc gtcccttatc
    23701 cagtagaagt agaagtaggc ggtgccacag tcttcgtctt acctgttgaa ggattccatc
    23761 aattttagga ggagctgagc tatgaatcaa gaaataaact tggctcaaat gcaaccgatc
    23821 gtgtatcagc agcttcaacg gagttttgag catgggcgta tcgctcatgc ttatcttttt
    23881 gaaggggaga aaggaacagg aaaacatgaa gtaggtatat ggcttgcaca gcacctgttc
    23941 tgtacccaaa tgaaagacaa tctgccttgt ggaaaatgca acaattgtca gcggatcgaa
    24001 aagaaagaac atccagatgt tttggttatt gaaccggaag gacaaaccat caaagtcgat
    24061 cagatcagac gtttgcagac ggaattcagt cgcagcggtt acgaatcaag aaaaaaagtt
    24121 tttttgatca aagaagctga aaaaatgaat agcagtgcgg caaacagttt gttgaaattc
    24181 ttggaagagc cccctggaga ttttttagcg gtgttagaaa cagatgcagt aggaagaatt
    24241 ctccctacta ttcaatctcg ttgtcaagtt ctgcattttc aagaactttc gaaagaagca
    24301 ctgatccatc gattgcaaga agagcagatt ccattggaaa aagcaaaact tttggctttc
    24361 ttaacaaaca gtttaggaaa agcagttgaa atctctcaag atgaatggtt taatgatgct
    24421 aaggacttga tttatcaatg gttcgtttac ttgcaaaagc aagatactca agcctttatc
    24481 tatgtccaaa aaaaattggt aaaatcattt aaagaaaaaa cacaacagtt tacaggatta
    24541 tctgtcttga tgttttatta tcaggaagca cagcgtcaag cagtagcaaa cggcgaatat
    24601 ggcaaagtga aacagataaa ccagataatt gaacggatat tgatcgcaga gcagaaacta
    24661 cgcagcaatg tcagttttca ggctgttgcg gaacagttcg ttttgcatac gattttttag
    24721 cttgccgcaa aggatgatga tcaaggagga tggaaatggt agaagtagta ggtgtccgat
    24781 atagagatgc aggtcatatc tattattttg caccaggaga ctctgaatat gtttatagtg
    24841 acaaggtatt agtcgaatct cagcaatcct tacatattgc tactgttgtc cttcctaaaa
    24901 aagagattga tcccaatgac cttcctgaag ctatccgtcc gatcttgcat aaagcgtcac
    24961 aagctgaatt agaaaaagac aaaaaaaaca aaaaagatgc ttatgatgca ttgaaaatcg
    25021 caaaagaaaa agtgcagttg cgtgagttgg aaatgaaaat ggtcaaagcc gaatacacat
    25081 ttgatcgaaa taaattgatt ttttctttca cagcggacgg gcgaattgac tttagagaat
    25141 tggtaaaaga tctggccgct gtatttcgta ctcgtattga gcttcgacaa attggtgtcc
    25201 gcgatgaagc aaagataata ggcggaattg gtccatgtgg gcgtccatta tgctgttctt
    25261 cttttttagg cgatttcgtg cctgtatcaa tcaaaatggc aaaagatcaa agtttatctt
    25321 tgaatccaac aaaaatttcc ggtttatgcg gacgcttgat gtgctgtctg caatatgaga
    25381 gcgaagaata cgagacagcg aaaaaagaac tccctgactt tggaaaagta attgctacac
    25441 ctgatggtaa agggaaagtc gtagggttga atctgcttag ccgtatcgtt agtattcggc
    25501 tgactggacg ggaaaatcct gtcgaatatg aatgggatga attgaaagaa tttatccaag
    25561 taggtgagat gaatggataa gagatcatta tacgacggat tgaatcaatt agaatcagag
    25621 ttgagaaata cattaacaga acttgttgaa atgaagcatg ctttgcatga gatcgtagaa
    25681 aaaaatacaa ctttggagat ggaaaatcaa cgtctgcggg aacacctgcg ggaattgaac
    25741 caagttgcac aagctccagc tgaaaatgtg aagcaggaac tttctaagtc tcgtatgaat
    25801 cttgaaaaaa tttatgaaga aggattccat gtctgctacg atttatatgg atcaagaagg
    25861 gacaatgacg agccttgtgc gttttgtcta gaagtgatct atcgcgaaag cggtcgatga
    25921 ccaataacaa gaggagactg gagcggagaa atactgctcc agtcttttta ttaaggagat
    25981 tgtgacagat gtataatcaa aaaagtttca agggaaatcc ggtatatggg gatttatatc
    26041 ttgtaccgac gccaattgga aatttagaag atatgagcta cagaagtgtt cgtatgctgc
    26101 aagaatccga tcttatcgca agtgaggata cgcgtaatac acaaaagtta ttgaatcatt
    26161 ttgaaatcca tacaccccaa aaaagtttac atgaacataa ttataaagaa cgtgtccctc
    26221 aattgattgc tttgttaaaa gaaggaaaaa cgattgctca agtcagtgac gcaggaatgc
    26281 cttccatcag cgatcctgga catgagctag ttttagcctg cataaaagaa gggatttctg
    26341 tgatctctat cccaggacct acagctggga tgacggcttt gattgcttca gggctagtgc
    26401 ctcaaccatt tttattttat ggcttccttc cacgtaagaa aaaagaacag aaagaaacat
    26461 tgaaaaagct aaaagaacag acagttacgc tgatttttta tgagtcacct tatcggatca
    26521 ctgctacatt gacgaatttc ttagaaattt ttggaaatcg ccaagtcgtt ttgtgtcggg
    26581 aattgacaaa ggtccatgaa gaatacctta gaggaagtac ggaagagcta ctcgcttata
    26641 tcgaagaaca tccagtaaag ggtgaatgtt gtctgcttgt agaagggaat ttgttcgaaa
    26701 tgccagtgac ggaagttgat gaagagttgg gaacgttaaa ggaacaagta gaagcaaaga
    26761 tcgcttcagg cgaaaaacca aatgctgcta tcaaagcagt agcgctgaaa aatgggctga
    26821 aaaaacaaga agtctaccgt cagtaccatg aattagactg agatagtata agttgtctat
    26881 aatgaaagac taatgaggaa atcagctact aacttttcaa atctcttgaa cgcgaacgaa
    26941 tgttcttgta taatgaataa gaggtgagga tgatggagct gcaatttcct ttacataatg
    27001 atacatcaag aaaaatcatt catattgata tggatgcttt tttcgcttcg gttgaagaac
    27061 gagatcatcc tgagtttcgc ggacatccat tagtaattgc cagacacccc agtgacactg
    27121 gtgggcgtgg agtagtgact acagcaaatt atgaagcaag aaaattcgga attcattcag
    27181 caatgagtgc acaaaaagcg tatgagcttt gtccgcaagc catctttaag cctggcgacc
    27241 atcataaata cagcgatatt tccaaacaag tacgggaagt attctatcgt tatacagata
    27301 ttgtggaacc agttagtatc gatgaagcgt atcttgatgt gaccgaaaat aaaatcaatt
    27361 gcaagtcagc catcaaaatc gcccgaatga tccaagcaga tatttgggag agtgtccagc
    27421 taacatgttc tgcgggagta agttacaata agtttttggc caaattagca tcagactatc
    27481 aaaaacctag aggaatcacg gtagttgccc ctcaagacgc tgtgccattt ttaaaagctt
    27541 tgccaattga aaaatttcat ggggtaggga agaaaactgt acctagaatg catgagctag
    27601 ggatttatac aggaaaagac ctatatgaat gcacagaaat gatgttgatt cgaaattttg
    27661 gaaaaatggg ctattctctt tatcgtaaag tcagaggaat ccatgattct ccagtaaacg
    27721 ttacaagaga gcgaaaatct gtgggaaaag agcatactta tgggcagccg ttgcaaacgg
    27781 aagaagcagt gctgactcag ttgcgccaac tagctgaaaa agtagaagaa gcactgagaa
    27841 gagtccaaaa acatgggaaa acagtagttt tgaaggtcag gtacacggac tattctactg
    27901 tgaccaaaag agtgacattg ccagaatata tctataaaaa agaagcactc ttttatcaag
    27961 caagccttat atgggaggaa atcttgggag tagaaaaagg aatccgattg ttagggatta
    28021 ctttgacgaa tctggacccg atgacttatg aaaacatcgt ccttccatta tgggaaaatc
    28081 aagaaatata aaaacagctt tgagagttga aaccggaaat ccgttttctc tcccaaagct
    28141 gtttttatat caagtcttgc tttttgattt gttcataggt ttctgttgca tccgtacatt
    28201 tttcccaaat cacaacttga ttggattcta aagatttttg gacgtcaatg atccgctggt
    28261 tgctacttcc gcgaaattgt agttttaggt cacgtttgct taattcaaag cgtccgtcaa
    28321 caaggatatc gatttgcgaa aggagttcta atttatcttc tgaatcttgt agcaattcat
    28381 caaaggtata gcctgtccat gaccagatat cttttgtcgt accgaactct tttcggatac
    28441 gtcgtacgac ttgcagacaa acatctgtat tcaaaaaagg ttcgccacca agtaaagtta
    28501 atccttgcac gtaatcgttg cgcaagtcag cgatgatttg ttcttctaat tcaggtgtga
    28561 atggtttgcc atatcggaaa ttttgtgcag ctttgttgaa gcagccttcg caggcaaata
    28621 agcagccact aacgtaaaga ctgttgcgga caccttcacc atcgacaaaa ttaaacggtt
    28681 tgtagtcagc aatatagttt tggctgtagt cttttgctaa ccattctttt ggttttggat
    28741 ttctcataaa aaaacttctt tcatttaagg ttcaaagata tagctattga agataaaatc
    28801 tactcgacca cataaataag aacaaactga cctaaatcca ttgttcaaga aagcttgttc
    28861 ttattttgat agagggcgtt caaagataca aagccatcga aagagggtgg tgaaaaaaga
    28921 cttgtcatag cctcttattc tgaataaacg gtgttcaaaa gcttgcatct gcagtaataa
    28981 gcccaactaa acgaaaaata tgaggaacta tttttgttta gctattctta ttacttgatg
    29041 caggacagct ttttcacaac ctcatttcat gtgtttcaca cgtgaggaaa tttctttatg
    29101 gcggccatgt accatcggac gagcttgcgg atttcctaaa tagccacaag tacgtttgac
    29161 tacatcacat gtttttggat catgatttcc acattctgga cattcgaacc ctcgttctgt
    29221 cggtttgaaa tcaccttcga aaccacattg atagcaatga tcgattggcg tattcgtacc
    29281 aagatacccg attttgtcat atgcaaaatc ccatacagct tctaaagctt tcggattttg
    29341 ttggagcata gggtattcac agtagtggat gaatccacct gaacaatatt ttggataatc
    29401 cttctcaaat tccaattttt caaatggcgt tgggttttta cgcacatcat agtggaaact
    29461 gtttgtataa tattctttat ctgtgatatt ttcgatgatg ccgaattttt ctgtatccaa
    29521 acggcagaaa cggtcagtta gactttcact tggagttgaa tagacactga aatgataacc
    29581 atattcatcc ccccatgcat ctgcattagc ttttaattct ttgacgatat caagtgtgaa
    29641 agcttttgct tctgggtttg tttcccattc gccaccgaag aaagctgaag caacttcata
    29701 caatccaatg tagcctaacg atacagtagc tcgacggttt ttgaataatt cattgacaga
    29761 atcggtacgt gaaagacgtt tcccgaaagc accatacata tataaaattg gcgcatttgc
    29821 tggaatggct tctttacaac gttctacacg atagagaagc gcatctttca tgatttctaa
    29881 gcggtcagac agtaactgcc agaatttatt ctgatctcct tttgattcga tcgcgattct
    29941 tggcaaattc aatgtaacaa cacctaaatt cattcgtccg acatttactt cttgaccatt
    30001 ttcgtctttc cacccttgca agaatgaacg acagcccata ggtactttga aacttccagt
    30061 taattcaacg attttatcgt aattcagaat atcaggatac attcttttcg ttgcacattc
    30121 caatgctagt tgtttgatgt cgtaattagg atcttcaggg tttaagttca cacctttttt
    30181 caaagtgaag atcagtttag ggaagatagc tgtccgcttt tcactcccta atccattgat
    30241 tcgaatctgt aaaattgctt tttgaatttc tctttcaaac cagttttctc ctagtccaaa
    30301 tcctaatgaa gtgaaaggag tttgtccatt tgaagtaaat aaggtattga tttcatattc
    30361 caaactttgc atagcatcga agatatcttt tttggttttt gcttttgcat attctttttg
    30421 gcgttctggc gaatcgatcc attcttccgc gtcttttaga tgttttttat agttcaattc
    30481 tgcaaaagga gcaagcaatt catctgttcg gtcggcagaa cagccaccat attgacttga
    30541 cgctacattg gcaataatct gtgagatttg tgctgtggcg gtctggatag attttggcga
    30601 ttcgacttcg gcattgccga ttttgaaccc attgttcagc atgcctttga aatcaatcaa
    30661 gcagcaattc gtcatcggtg tataaggatg ataatccaaa tcatggtaat ggatatcgcc
    30721 tttttgatgc gcatttgcta tatgtggtgg caacattttc agtccgattg attttccaac
    30781 gatcccggct gtcagatctc gctgtgtatt gaaaacatta ctgtctttat ttgcgttttc
    30841 gttcacgaca gtttggtcct tgttgattag cttttcaatc gaaaagttga tatctgttgc
    30901 tttactgcga gcaaaatctc gtttggtacg ataattgatg tactcttctg ctaattcata
    30961 ttcgtttttt tctagtaata tatgttccac gatgttttga atctcataga ttttgacatt
    31021 ttcagcaaac cggcttttga tttcttggac aaccaagtct gcaatcattt gaattttttc
    31081 ataagtaagt ggtgataatg agccgtgaat tttcttttct gctttgatca gtgcttcatt
    31141 gatcttttgt tcatcgaaat caacatggcg gccatcgcgt ttgatgactt tcatctggga
    31201 tgtctctggc tgctgagtaa cgacagtttt ttcttcataa tttactttca ttatcttcaa
    31261 ctcctgctaa attgttcttg tctatcataa aacaaaatcg gggaaggagc aactaaatat
    31321 cggctatata tagtattcaa tgtgctgtat aattggattt ctatactata tgtagtgtat
    31381 attttattgt gaaaaaaggt gaaaacttgt agctggtgga tttgtttttt gcatatttaa
    31441 ttttgctgaa taaaataatt gaataaaatt ttttattcag tccaagtaaa agtgacaaaa
    31501 acataagagc aaattattcg tagatatgct actatgcaaa taaatagaaa tgaaaaatga
    31561 aagaggagga gaagaattgg aaaagatgat cgaagtgaaa catctaagta aaagctacgg
    31621 cagcaaactt tctcccgtca aagccttaga taatatctca ttcacagtag aagaaggcga
    31681 gtttgttggg attatgggcc caagtggtgc aggaaagaca acgctgatga atatcttgtc
    31741 cacgatcaat ttgcctacga tgggcagtgt ctcgatccaa ggaaaagaaa tcacaaaaat
    31801 gaaaaacagt gagctcagtg actttcgtcg aaaaaaacta ggattcattt ttcaagagtt
    31861 caatttgatc gataccctaa atgcaaaaga caatatccta ttgcccctag cagttgaacg
    31921 gatgactaaa gaggaaatgg aaaagagagt aagacatgta gctcaattgt tgaatataga
    31981 agaactgctc aaacggtatc ccgatgaact ttctgtcgga cagagacaac gaattgcagc
    32041 tgctagagca cttgttgttc agccacaagt gatttttgca gatgaaccaa ctggctcttt
    32101 ggactcgaag tcggctacag aattgttgaa ctacttgaag accatgaatc aaaaagaaaa
    32161 agcaacgata ctgttagtca cccatgatcc atatacagca agctactgcg atagaatttt
    32221 atttattaaa gatggtgtga ttttttctga agttgtcaga agagggacaa gaagagagtt
    32281 ctttgagaaa gtaatagaca tgcaggcgac gattggcgga ggaggaaaaa tgaatgctgt
    32341 ataaactagc cgttaaaagt ttttttcggc agtcacgagg ctatcttgtt tattttttca
    32401 gtttgacttt atccgtaatg atctattatt ccttcagcgc aatgacctac gatcaatctt
    32461 tagtcagaag agcaagtcaa gatacttcga tagatggtgg attgagtttt ggtggattta
    32521 tcattactgt tgttttgctt tttttcgtat tcagtgccaa ccgatttttc ttgaatagaa
    32581 ggcaaaagga aataggcatc tatcagcttt ttggtatgaa taaactgcag atatcaggta
    32641 tatatgtgtt agaaatcatg ataattggat tgttcgcctg tatttctggg atattactcg
    32701 gtattatttt ttccaaactc ttttcaatga ttttggttcg aatgatggat atggatctta
    32761 ctagtccatt ttttatctcc attccatcag tagtagatac tttatttgct tttttcatca
    32821 tccttttagc agtatctgta tattcaatat ggaaaatatg gcgctatcca atgattcgaa
    32881 gttttggaga aaaagaacaa gcagatagtt caacaatgcg cttcaggaca aggcaccgat
    32941 tactcgcagt catcgggtta ctacttttaa cgagcggata ttttggagct agtcatttta
    33001 gggaaatcac ttcatggatg atcagttatc attatcttga tacattagtt ttcttttttc
    33061 cttttttaat ttttttcgtt tgtgtggtcg gtacttattt gtttttctgt tatacatttc
    33121 ggttattttt gatcatattg agtaaatgga agatttatta ctatggtatc aattttttga
    33181 taatgggtaa cacgcaagtt catttgctca aaagttggcg tacaaattct ctgattacag
    33241 tcattctcgg aatttcttta atgacaattg gcggcacggc tagtatctct actattttat
    33301 cctaccagga gaaagtttcc tccccgatgg actatcagtt tgatgttgag acagagaaaa
    33361 aaatcaagcc gattctagca gaggaagggc agaagataac taaagaaatc accttgcatt
    33421 ataaagtcat tgggagtatc tatagtcaaa atctcgaaga acctatgttt caaactgcta
    33481 atctgatcac ggaaacacaa tatcaagcct tcagaaaagt taatccagat ttgccgaaca
    33541 tttctttgaa atcacctggt catactgttt tgcttgatca aatgcaaact ctttttcgga
    33601 atatttcatt atatggatct agtattggac tgccagataa tcaagaattg aagattcaac
    33661 aaatgcttcc aagtgtatta ggtgacgaaa caatggtata tctaagtcct gtactgattg
    33721 tagatgatac cgtttttgaa aaagcaaaag gtgtggatta tcaagtagtc aatgtagatg
    33781 tatcaggtgg aaataatgaa aaaatagatg aacgactgag caatgacctg tcgataaagt
    33841 ggctcaatgc tgtttattat tcttatgata tcgtaaatgg aagtctacaa ggagaaatta
    33901 gcacaaagaa acttccggat gaccaaatga aggaagcaca atttagccaa gaaagctctc
    33961 gtttgaattt ctcttccagc tattcgaaat tgcgtgcagt taatcggcga atcggtatca
    34021 atatttttgt cacactattt gtcgggatga tcgtgattat tgctactggt agtattttga
    34081 cagtccggca attagcagaa gcagaggaag aaaaagaaaa ttatcaccta ttgactaagc
    34141 tggggattcc acaaagaagg atcagacgga tgatcttcga gcaaaacgca cttaccttct
    34201 ttccacccat gatattaggg atcactcacg cagtatttgc aatcaatgtc ttcactcagt
    34261 atataaaaac tgccgattac tggttggctt atcttgtgag tggcttattg atccttatct
    34321 atcttttgct ttatgtcatt acgagtcagt tatattgtcg gattatcgag gaatgatata
    34381 cggaataaga caagataata aatagaacta aattttgaat accatttatt tggaataaga
    34441 ggttgtgaca cgatttttgt cacaacctct ttatttttta tattttttaa aagtacgaga
    34501 aattataaag aaaaaagcag tgatttgatg tgctattttt ataaaagcta tgtgacaaaa
    34561 ctgttagtaa aaaaacggtt tctgtaagct agacacatgt ttctttttgt caaaattagt
    34621 tacactttgt gtgaggttat atgaggaggg aatccaatga acaaatggcg ttctaatcca
    34681 aaattgaatt attggggaaa attcatcatt cgaacaattt tttatagtgc agtactgctg
    34741 ttcctgattt atctgtatca ctataaaaat attcaaggag gaacttttat ctataacgaa
    34801 ttttaggagg aagagtatgg aaatcaaaac gattattgaa gcaatcgatg attggggaat
    34861 aaatgaacct gatcgagttg cttatcaggc ggatgagacg catacttttg gtgaattgaa
    34921 acaagcatca gacgcacttg cttattattt agaaaaaaag gtagaaggcg atggaccaat
    34981 tgtcgttttt ggcaatttgg agtttgaaat gattgtctca tttctagggg ttgtcaaatc
    35041 tggccatgcc tatttgccga ttgaagagca tacacccaag gagcgtatcc tatcgatctt
    35101 tcgggtggct aaaccatcta tggtcatcag tatcggggac tggatagaag atattccttc
    35161 ggttcctgtc ataacaaaaa aagaatttca agaaatcaag aagataccag taggatttta
    35221 tcccagaaat agcgtaaaag gtacagaaaa tttttatatt atttttactt ctggtactac
    35281 tggagaacca aaaggcgtac aaatcagtca tgataatcta gtcagctttg ttcaatggac
    35341 gatggaagac tttgggatca agcctggtat gcatttctta gcccaagcgc cattttcttt
    35401 tgatttatcg gtattttcta tttatcctgc attagtttct ggcggaatgc tgaagccgtt
    35461 aaataaagca gttgttcaag actttcgtca attattcgcg acattgccag ccttgaagtt
    35521 gaatgtgtgg gtttctactc catcatttat ggatatttgc ttgatggaac ctacattcaa
    35581 tggagaaaat gtgccggaat tggacatgtt tttattttgc ggagaagaat tgacgaaaaa
    35641 aactgcagaa agtctacttg atcgattccc aaatgcacgg atatataata cttacggtcc
    35701 aacagaagca actgtagcta tttcaagtat tcagattgac cagcatgtac tagatagtta
    35761 tgaccgacta ccgattggct acgtaaaaaa agacacgaaa gtttctattg tccaagaagg
    35821 taaacctgtt tcaaaaggag aaacgggtga aatcattatt gcaggaccaa gcgtatcaaa
    35881 aggttacctg aacaatcccg aaaagacagc tgctgcgttc ttttgtcaaa atgacttagc
    35941 aagttaccat acaggggatg caggtagact agatgaatat gggcttttat tttatgaagg
    36001 acgtatggat cagcaggtca aattacatgg ataccggatt gaactcgggg atatcgaaca
    36061 ttatctgctc caagacaacc gaatcaaaca agctgtcgtc gtaccaaaat accaaggaac
    36121 aaaagtacag cagctggttg cttttgtcgt attagaaaac aaaacgaaac agccggattt
    36181 tcagttgact aaatctatca aagaacaatt actagaagtt gtaatggatt atatgattcc
    36241 tcagaagttc cattatgtcg atctgctgcc tcagacagtt aatggaaaaa tcgatcgaaa
    36301 aaaattaaca gctgaggtga atcctgaatg atgttacctc atatgatccc ttatgcagag
    36361 ccgttttatt tttttctttt agccattgcg cttgtaccga tcgttctatc tttattgata
    36421 tgggaaaaaa gattaccagt ttatcaatca gcagtgacac ttttcttttt atttgtcagc
    36481 tttggaggta attattggca acagggagcg gcgctgattg ggtatgtttt gtggcagacg
    36541 atacttgtat ggggctattt tgtttatcgg caaaggaaaa atcagacagg atggtttttt
    36601 gccgccgtta ttttagcgat aattccactg tttttaacga aagtgactcc tttttttgaa
    36661 catggaaaag catcgttact tggtttttta ggaatctctt atttaacctt taaatctgta
    36721 caaatgatca tggaaataag agatggcatg atcaaaacat ataagccatt tcgttatatc
    36781 cagtttctgc tgttctttcc aacaatctct tctggaccaa tcgatcgcta taggagattt
    36841 gaaaaagatc agctccatcc gcctgttcgt gataaatatg ttgatctttt gggaaaaggc
    36901 gtccaaaata tttttgtcgg ttttttatat aaattcatta tcggttatta ttttggtcaa
    36961 gtactgcttc ctgtagtggg acgcattgcc ttacagcatg gtggtatttc atgggcatta
    37021 gtaggttata tgtacgtgta cagtatgtat ttgttttttg attttgctgg ctatagctta
    37081 tttgcagtcg gtacaagcta catgatggga tatcagacac caatcaattt caataaacct
    37141 tttttatcat ggaatatcaa agaattctgg aatcgttggc atatgacgct gtccttttgg
    37201 ttccgtgatt atgtatatat gcgattgatg ttcttcatga tgaagaaaaa attttttaaa
    37261 agcaagatcg taatgtcaaa tatcggttat tttgctttgt ttcttctaat gggtatatgg
    37321 catggattga cgtggtacta catcgtatat ggactgtatc atgcttgtct gatctgtctg
    37381 acagatgcat ggatccgatt taagaagaag cataaagagc agttgccttc taacaaattt
    37441 acgcatggct tagccgtatt tttgacattt caagcagtat gttttagctt cctgattttt
    37501 tcaggatttt tagataaact tttttttagc taacgaatag tgacatatta atttatataa
    37561 caaaggagaa aaggaaaatg gaagaacaaa ttttatcaat cttagaagaa ataacaggaa
    37621 cagatgaagt aagaaaaaat cgtgatattg atctatttga tgaagggttg cttgattctt
    37681 tagcatctgt tcaattatta gtcgagctag acggagagct ggatatccaa gtccctgtat
    37741 ctgaattcga gcgagaggat tggagcacac cgaataaaat cattcaacaa gcaactgcat
    37801 tgaagggttg agtatgaaaa agaaactttt tgctattttc ggcccggttg ttttagctgt
    37861 tggcttactg tttgtgtttt ttacctcctc ctatcaagta gatatctcta atccagaaac
    37921 gattagacgt gcctcttctt ccatgtcagt gaacgtgttg aaaggagatg cgataaaaaa
    37981 ttatgcatta tctgaaaaac aatatattcc tttttttggc tcaagtgaat taagcagaat
    38041 cagtccattt catccgtctg ttctggcaga aaaataccag cgaaactatc gtccattttt
    38101 gctgggagca cccgggacac aatcgttaag ccaatatatg atgatgcgtt ctgcaggaga
    38161 tgctatgaaa aacaagaaag tcgtctttat tatttctcct cagtggttcg ttaaaaatgg
    38221 tgtaaaaact gattacttta atacgtatta ttctgaactt caaacatacg actggttgtt
    38281 ttcaatgaag aaagtaacgc ctgctgatcg ctatttagct agaagattat taactttctc
    38341 aaaagtaaaa gagaatgata cgttgacggc gattctgcaa acgatcaaaa aagggaagct
    38401 acctttacct gaatctttga atcagctgcg cagccaatgg aacatgctga agagagagga
    38461 tgaagtattc agcaatattg ggttgaaaga tcgacagcaa aaaatcgatc atgagtcaaa
    38521 acgattacca aaacaatacc aagaaacaga attatccatt ttagcaaatc aaattggaga
    38581 acgagaaacg acgaataatc cattcgggtt gaaaaacgat ttttatacgc atcgtatccg
    38641 agcccacgag ccagagctca aacagtcaca aaaaaattgg gattatcgtt tttcgccaga
    38701 attttctgat tttcagctgg tcttggatca attagcaaaa aaccataacg aagtattgtt
    38761 catcatccca ccggttaacg agaaatggag cgattatacc ggactatctc aagaaatgtt
    38821 acaaggattt gccaaaaaga tcaaatttca attaaatagc caaggattca atcgaatagc
    38881 tgattttgtc aatcaagcgg gtaccaacta ttttatggaa gacacgatcc atctaggctg
    38941 gaaaggctgg ttggctgcag atcaacagat cagaccgttt ttagaagaaa atcatattac
    39001 tgctagtaag tatcatcttg acgatgcttt ttttagcaaa tcttggcaac atcaaatacc
    39061 agataaacta caattaaaat aaagaaaaca ggcgagtgtt gctcgcttgt tttctttatt
    39121 tttgaatatt ctgaataaaa gataaaaata ccaagtttgc ctgaacaaaa atcataagca
    39181 atggtataat cacaatatga atatagaaat gggggaaaac atttggatac aaaagaagaa
    39241 cagctactta ttgatttaac ttctgcaaaa ggagtacctg gaaatgaaga agaggtaaga
    39301 gaagtattca gagaatatgc caaaccattc gctgatgata ttttttatga cggtttaggc
    39361 agtgtgattg ccaaacatgg tgctggcgga ggccccaagg tctttatttc tggacatatg
    39421 gacgaagttg gttttatggt aacaaaaata accgaaaaag gattcttgga attccaaact
    39481 cttggaggtt ggtgggggca agtcatgctt gctcagcaag ttgaaatcaa aacacgtgaa
    39541 gggaaaatcg tccacggtgt gattggttcc aaaccaccac atgttttaac acctcaagta
    39601 agaaataaac cttatgaaat aaaggatatg ttcattgata ttggcgcatc aagccaagaa
    39661 gaagccaaag agtggggcat acgtcctgga gatatggtca ctccatatat tgaatacaaa
    39721 cgaatgaatg gcagtaaata tttactggca aaagcatggg ataatcgtat cgggacagct
    39781 gtttctctgc gagtattaga gaatctatct aaagaagcac acccaaatgt tttattcgca
    39841 ggaagcgatg tgcaagaaga agtaggcttg cgaggtgctc gaacaagtac acaccttgta
    39901 aatcccgata ttgcttttgc tttagatact ggtacagcag gagacactcc aggaatgaca
    39961 ccaaaagaag cagattcgat tttaggaaaa ggcccacaga ttttgatttt tgatgcatct
    40021 atgatcccgc ataaaaaatt attgaatttt gttattgatg tagcagagga aatggatatt
    40081 ccattccaat atacagtgat ttctggcgga ggaactgatg caggacaaat gcatttgaca
    40141 agagatggtg ttccgtctgt agccattacc gttcctgttc gttatctgca ttcccataca
    40201 tcagtgattc atgaagatga ttatttgaat acagtgaaac tagtcacaga attagtgaaa
    40261 cggcttgata gtgaaacagt tgaaaaaatt cggagttatg aataatgaat aatagaatac
    40321 ctatttctga atatcggaaa aaagcacgag tttctttgga aaatcaatgg ggaatcaata
    40381 cttggcttgt tttcttatct ataatattta cgagcgtcat ccagaacttt tttgacggtt
    40441 tcagtccttt tccggaagaa agcatgcagc agcacttatt gagttttcta gtcgataatt
    40501 tacttttatt cgcattcaac tatgcattgt tttacattgc gctagtagtt gtacggggtg
    40561 gcagaccaaa aagtaacctt ttattttcag ttttccaaag aaagtatttt ggttcgatac
    40621 ttggcatcaa tttgatcaat tcagtcatca actggctact caacagttta atctttctgc
    40681 ccatgttttt aatcagcgga gtgagtactt atatgcagct gcttttcagt tcaaatgcgg
    40741 tacctacggg tgtcatgcca gaagattttc ttgatatcag ctttgtttta tcgatgatag
    40801 ggatgatttt tgtttcagca ctcatcatga gtattgttgg cggtttattc caatttgctg
    40861 catggacaaa gttcgactat cctgatttgt cggttattca atgtttgaaa tatggctggt
    40921 ttttattgaa agatagaatt ggtacgtata ttttgttgca gctgtctttt atcggctggt
    40981 atttgctagg agcaatcgct ttgttcatcg gtcttttgtg ggtcattgtt tatgtgaatg
    41041 tgtcagttgc agaattttat gaacaagcac ggattgaaaa aggaggtcca gacgcctatt
    41101 ttacttagca tgtaagcagt ttattcatca aaaaaataac ttttgtggta ggataaatct
    41161 ttgaggggga atgtacgatg aaattaactg ttttaggatg cttaggagcg tatccttata
    41221 aaaatcaagg gacaacaggt tatttgcttc aatctgacgg gttcaatttg ttgatcgatg
    41281 caggtagtgc tacgctgatc aaattacaag aacaccttga tccacttgat ctagatgctg
    41341 tgatccttag ccattatcat catgatcata ttgctgacct tggtgtatta cagtattact
    41401 ggcaacttca tcctaagaga aatccggaga cattgctttc aatttatgga cacacaaaag
    41461 attccattca ctttgaagaa ttgacgttgg aaaatgtctc aaaaggacat ccttattttg
    41521 aagctgaaga attaaaaatt ggtccttttc tgattacttt tatggaaaca atccatccag
    41581 tagtatgtta cgccatgcgt atcgtagaag aaacaacggg gaaagtattc gtatttacag
    41641 gtgattcagg ttatttggaa agtttcgctg attttgcgaa agatgctgat ttgtttctag
    41701 cagataccta tctttttgaa ggaaatgaac atcatcatgc ccatttcact tcaaaagaag
    41761 ctggagaact tgcgaaatca gctaacgtaa agaaacttgt acttacacac ctacctcagt
    41821 ttggtgattt agaacaattg aaaaatgaag cgcaaaaggc agcaggcaaa gggattgata
    41881 tagaacttgc tgcagttgac aaagtgttta acatttagga ggaagaacat gatttttgta
    41941 ccaaatgaaa accacgaccc aagaatcaat ttagcaattg aacaatattt gctgcaagaa
    42001 atgcctttag atgaacctat tttgctcttt tatatcaatg aaccttcaat catcatcggc
    42061 agaaaccaaa atacgcttga agaaatcaat cgagattatg tggaagaaaa tggcattcat
    42121 gttgtccgtc gcttaagtgg aggcggagct gtctaccatg attttggaaa cctgaacttt
    42181 agttttatca tgcctgatga tggggattca ttccgtgact ttgcgaaagt aacacagcca
    42241 atcatccaag cacttcatga attgggtgtt gctggggcgg aattgaaagg ccgaaatgat
    42301 ctagtcattg atggcatgaa attttctggc aacgcgatgt atgctaccaa cggtcggatg
    42361 tttgctcatg gaacaattat gttcgactct gatatcaatg aagtagtcaa tgcattaaaa
    42421 gtaaaaaaag acaaaatcga atcaaaagga atcaaatcga tccgttctcg tgtaacgaat
    42481 atcaaaccat ttcttccaga agaaaagcaa aaaatgacaa cagaagaatt tcgtcaagaa
    42541 atcttgttga aaattttcgg cgtagagtca gtagaagaag taaaaacgta cgaattgact
    42601 gatgaagatt ggaaaaaaat caatcgaatc tctgaggaat attatcgaaa ttgggattgg
    42661 aattacggga agtctccaga tttcaatttc tctcgccaaa aacgtttttc tatcggttcg
    42721 attgaagtcc atttgaatgt ctcagaagga atgatcaaag aagcaaaaat ctttggcgat
    42781 ttcttcggac taggagatat ctctgacgtg gaaaaaaaat tggttggaca gaaatatgat
    42841 aaagcatctc tatcaaaaat agtcagccaa attgatgtga agaaatattt tggtgcgata
    42901 gaaccagaag aattgcttac cttactttat tgaaaataaa aaatagcgga atccacatat
    42961 atggattccg ctatttttta tacaggattc gggtaatttc gagagagttg aacagtgatc
    43021 cgatcaatga aagtatcttt ttcctgcgtt agtgttcctg tgtaacgatc cgctacgtct
    43081 ttcatcatca ttaagtcaaa acgttcctga tcatctaatt cttctgtgat gcctcgacta
    43141 aataggtttg tatcgtaatg tgcactatgt tcgcaacaaa tgacgaaatg ttcctcttgt
    43201 tcgtgtatcg acaaataaat ttttcgatac agtggatttt cgatggcatg agtttctcta
    43261 aaggaatgct ctaaagcgtg gatcaaaagt tgagccagat cgtcatcgtt gataggaagt
    43321 tcttcgggaa gatctgcttg gaaggttatc tgaatattcc gtttggcagc taacttctgg
    43381 taacgcgcta agataagatt tgtcacctgg tggttgcaaa aagaacacag catttcgaaa
    43441 ttttttgctt cttctaatat cttttccaca tagtttttag cttttgtttg gtccgtatgc
    43501 attaaatctt gcagattctc catctcttgg acaaattcca ggcgaagtgt atttacttct
    43561 tggatatgct gtcgaagctg ctcataatgt tgctccaaca aatcattttt gatttttaag
    43621 aaatctactt gtctccgatc tttgtcggta tttaagatgt gatcgattag attgtagctg
    43681 ataataacga ttagaatgat aatatataaa atcgtagacc aattgatcgt tgtttgatag
    43741 atacctaatg ccgtttggat gtaaatcaag caatggataa tggctaccaa gacgatccaa
    43801 ggtgtacaag ctacataaaa acggttattc ttatatgctt cgccaatgca tgcaagtgaa
    43861 gtgactaaag tgcttataac actagcaata ttagcaatcg gcattaattc aggaagttct
    43921 gcaggagtaa aagttgcata aagcagaacg ccgataaaaa tcaaaagttg gaacgtacaa
    43981 aaaattccgt accatttttt atagtaaaac attcggctcc aatagtagca gatgatgaaa
    44041 gctgacgtta agataataaa taaatgagat aaaaaagaat gaacgaatgg ttcaaaaaga
    44101 ataccactta aaatatcttc tgatacagct tcaaatccta atgccaaagc aaagcaactt
    44161 aagagaataa gtgagtattc aagacgtttt tgtttataca tcaagtagat cgtatagaaa
    44221 aaggtcccta tagcgataaa aacagcgatt atcatcgtca atacttgcgg cactgattgt
    44281 gaataaataa aactaatcaa tggtccagtt gttcctagat aaactttcgg tggcagtcca
    44341 gcatagttct gataaggaga tgagactgta agggtcaatt tttttccgat ataatttgct
    44401 ggtaaatcta tcacagccaa tgagagtccg ggattgcttt cttcctgata atctttccgt
    44461 tggtataaaa tcttttcgcc tagatataca gtaatccatt gatggtttcc tttgattagt
    44521 aaatttgctt cgtccaacgg tttttcaagt gtcgtttcca taatgaatgt ttcgtcttta
    44581 tccacggtag gaatcgtacg agtgtacttg ctgtcaaact taaaatcagg atcatctttt
    44641 gtatagaaaa cccacgaatc ttttatttct ttcatttgat tgctgtccgc aggagaaaaa
    44701 ataaaataaa agaaataagc aatt
//
LOCUS       DAFFSV010000010        41499 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFFSV010000010 DAFFSV010000000
VERSION     DAFFSV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.010"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(289..2475)
                     /locus_tag="LR110_000469"
     CDS             complement(289..2475)
                     /locus_tag="LR110_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL4821474.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSSVKKGK"
     gene            2693..3376
                     /locus_tag="LR110_000470"
     CDS             2693..3376
                     /locus_tag="LR110_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBL4821475.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3457..4176)
                     /locus_tag="LR110_000471"
     CDS             complement(3457..4176)
                     /locus_tag="LR110_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL4821476.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4488..4694)
                     /locus_tag="LR110_000472"
     CDS             complement(4488..4694)
                     /locus_tag="LR110_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821477.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4939..5541
                     /locus_tag="LR110_000473"
     CDS             4939..5541
                     /locus_tag="LR110_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL4821478.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5682..6962
                     /locus_tag="LR110_000474"
     CDS             5682..6962
                     /locus_tag="LR110_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL4821479.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6975..7704
                     /locus_tag="LR110_000475"
                     /pseudo
     CDS             6975..7704
                     /locus_tag="LR110_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(7773..9296)
                     /locus_tag="LR110_000476"
     CDS             complement(7773..9296)
                     /locus_tag="LR110_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL4821480.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9548..10729
                     /locus_tag="LR110_000477"
     CDS             9548..10729
                     /locus_tag="LR110_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL4821481.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10789..12417)
                     /gene="treC"
                     /locus_tag="LR110_000478"
     CDS             complement(10789..12417)
                     /gene="treC"
                     /locus_tag="LR110_000478"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBL4821482.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12814..15525)
                     /locus_tag="LR110_000479"
     CDS             complement(12814..15525)
                     /locus_tag="LR110_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4821483.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15535..16236)
                     /locus_tag="LR110_000480"
     CDS             complement(15535..16236)
                     /locus_tag="LR110_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4821484.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16422..17021
                     /locus_tag="LR110_000481"
     CDS             16422..17021
                     /locus_tag="LR110_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL4821485.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17102..18115
                     /locus_tag="LR110_000482"
     CDS             17102..18115
                     /locus_tag="LR110_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL4821486.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18223..19140
                     /gene="rbsK"
                     /locus_tag="LR110_000483"
     CDS             18223..19140
                     /gene="rbsK"
                     /locus_tag="LR110_000483"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBL4821487.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19168..19563
                     /gene="rbsD"
                     /locus_tag="LR110_000484"
     CDS             19168..19563
                     /gene="rbsD"
                     /locus_tag="LR110_000484"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBL4821488.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19563..20936
                     /locus_tag="LR110_000485"
     CDS             19563..20936
                     /locus_tag="LR110_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4821489.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21293..23437
                     /locus_tag="LR110_000486"
     CDS             21293..23437
                     /locus_tag="LR110_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL4821490.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23489..24529)
                     /locus_tag="LR110_000487"
     CDS             complement(23489..24529)
                     /locus_tag="LR110_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL4821491.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVRV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24806..26608)
                     /locus_tag="LR110_000488"
     CDS             complement(24806..26608)
                     /locus_tag="LR110_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4821492.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSVRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26622..27374)
                     /locus_tag="LR110_000489"
     CDS             complement(26622..27374)
                     /locus_tag="LR110_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4821493.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27383..27745)
                     /locus_tag="LR110_000490"
     CDS             complement(27383..27745)
                     /locus_tag="LR110_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL4821494.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27921..28793)
                     /locus_tag="LR110_000491"
     CDS             complement(27921..28793)
                     /locus_tag="LR110_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL4821495.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28844..29332)
                     /locus_tag="LR110_000492"
     CDS             complement(28844..29332)
                     /locus_tag="LR110_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBL4821496.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29470..30261)
                     /locus_tag="LR110_000493"
     CDS             complement(29470..30261)
                     /locus_tag="LR110_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBL4821497.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30316..30924)
                     /locus_tag="LR110_000494"
     CDS             complement(30316..30924)
                     /locus_tag="LR110_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBL4821498.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFVLKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30917..31234)
                     /locus_tag="LR110_000495"
     CDS             complement(30917..31234)
                     /locus_tag="LR110_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL4821499.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31390..32118)
                     /locus_tag="LR110_000496"
     CDS             complement(31390..32118)
                     /locus_tag="LR110_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBL4821500.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32277..33158
                     /locus_tag="LR110_000497"
     CDS             32277..33158
                     /locus_tag="LR110_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL4821501.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33395..34438)
                     /locus_tag="LR110_000498"
     CDS             complement(33395..34438)
                     /locus_tag="LR110_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL4821502.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34441..35109)
                     /locus_tag="LR110_000499"
     CDS             complement(34441..35109)
                     /locus_tag="LR110_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4821503.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35251..37587
                     /locus_tag="LR110_000500"
     CDS             35251..37587
                     /locus_tag="LR110_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4821504.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37702..38013)
                     /locus_tag="LR110_000501"
     CDS             complement(37702..38013)
                     /locus_tag="LR110_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821505.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNILALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38194..39669
                     /locus_tag="LR110_000502"
     CDS             38194..39669
                     /locus_tag="LR110_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBL4821506.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHYDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39716..40300
                     /locus_tag="LR110_000503"
     CDS             39716..40300
                     /locus_tag="LR110_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBL4821507.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQPKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            40302..40721
                     /locus_tag="LR110_000504"
     CDS             40302..40721
                     /locus_tag="LR110_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBL4821508.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(41002..41286)
                     /locus_tag="LR110_000505"
     CDS             complement(41002..41286)
                     /locus_tag="LR110_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBL4821509.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
BASE COUNT        12360 a         8316 c         7346 g        13477 t
ORIGIN      
        1 ttattagcta ataaaatata aaaaaaaatc aaaataactg attatttacg tttgaataat
       61 gagaattttt ctatatattc tctattttga taatagtatt ctcattttca aaataaataa
      121 gggaggtgtg acaaaattct tgtcacacct cccttttccg aataaactgt gttccagaaa
      181 tagcttcttc gaaaataagc tgaaatttca caaaaatttg agaagcaatt ttcgtacatt
      241 ccttcttctt tcttgaaact gaccacttct gtcacaccct ctcttctatc actttccttt
      301 tttcacacta ctcccggttt ctttgataaa tctcgatact ttcaattttc ttgaattttt
      361 catttgaggt acacatagat acagacgatt tcttgctcta gtcatcgcaa cataaaatag
      421 tcttcgttct tcttctatcg tcgtcactgg ttctgctttt atttcattaa agtcgattac
      481 tttcttttct gtcgctccgt tataaggcaa acttccttcg ttacaaccaa tcaaaaagac
      541 atttcggtaa ctaagacctt tacttttgtg aatgctctgg atacatagtg catcaagttt
      601 tccttgtctg taagcaatca agcgggtttt taattcttta gctgtcgctt gggtttcttg
      661 cgataaggct tggagtcttc tgaaagtagc atgctgcttt acttcttctc gtatccattc
      721 ttgatcactc tggtcgattt ccttagctat acgtggcaga tgaagatagc tttgaacaag
      781 cgaatagatt tcttgatgct ggtctgtttt acgcaacgct tcctcaaact ctcggatttc
      841 tgcagatttc gattttaaat tggggcttat cgacaaaata taatctgcaa cggctatatt
      901 ttgatattta ggagaaaagt attgatcgca ttcacttttt ttcaggtagc agtctgccat
      961 cagctgccgg agcaattgct ggtaggcctg atttttcttt tgcagattca gtgctgtttg
     1021 ccaatgaaca attttttgat agaaatgttg gaaagacttg ataccaaaga taccttcata
     1081 tggactatct gcttccagta caaaaggaat attcgttttc agcagagatt ggacaagctg
     1141 ctgcgtttgt gaagctgatc ggtataatat gattgattcc ttatatttcc catttttttg
     1201 aataaatgtc tgaatttgtt tgattagcca ctgactttct gctttagtat cagaaaacaa
     1261 ataggcttcc ataacacctg cttctttttg tgctggaatc atcgcctttg gtatccgatc
     1321 atttttatta tagctgatca atctatttgc tccttggaca atagctgccc gagaacgata
     1381 attagttaat aagaaaagag ttttggtagg atattctttt tcaaagttct tgatgtatgc
     1441 tggctctgat cctctgaatc catagatact ctgatcatca tctccaacaa caatcaactt
     1501 gatcctgttt tcctttgtca tccgtttcac gatttcccat tgaagtggat tagtatcttg
     1561 aaattcatca atgaatagat aatcatattt ttggccgata taatgctgga tctcctcatt
     1621 ctccaaagcg cttttcagat tttggagtac atcattgaac tctagcaatt gctggcttct
     1681 tttttgttct tggagttccg tgagcaatcc atacagtcgt tctgatatcg gctgtcgtgc
     1741 attcttatca atggaatcat atttttcttt aactaactga accatttctt ctactgaaag
     1801 gttcatattc tgccaatagt ctaaccgaga agcaatcgtt ttttctccat attgatcaaa
     1861 tatataaaga ttatatttac gtatcagctg ttgcaaaaca gcattcactt tttgtgcagt
     1921 atcctttgaa aagtctcctt gtattccttg cattgctact tcaggaaatt ttcgacaaat
     1981 ttcattgtat atcctaaaaa agatactatg gaatgttccg atatggacag cggtttttct
     2041 ctggctgaaa ttatctactc gtttttttag ttcatttgca gcgttccttg taaaagtgat
     2101 cattagtatg cgttcttcgt ctacttgttt ttgcgtcatc agataaacag cttttgcaca
     2161 gattgtttgc gttttgccgc tcccagctcc tgctatgatt cgaagatgtt tcttcatatc
     2221 atatgtgata gctgcttttt gttcttgatt gaagcgatta taggaacgtt tctccatcat
     2281 tttaaagaaa gagctgtttc cttcactttc ttttacttgt acttcttcct caataagtcc
     2341 gggtgcttcg ccataagccg cttcataggt agcaatgatt tcttcttgta gccgaaagac
     2401 ttgttgaagc tttttatact tgttctccca gcgtttcaat tctttttgta atcggagaat
     2461 ttctttttca ttcaactgat tgatttcctt cccatctttc ttctttgaat ttgtttattt
     2521 caagttggtt atttaaagtt tcgttgtcta ttttttcttt atactaacag acctttctaa
     2581 aaatgaaaat gggaagcgat atgattttag ttttctgaaa attttccctt tttttcaatc
     2641 gtttatgcta agataaggaa gacaaaaata ttcataaaag gtgatgaaaa aaatgcagac
     2701 ttcctttcac gaggggcgta caataggact tttgctgaca tttatcggag gagccatgga
     2761 cagttatact tatatccaat atggtgcttt tgcctctgct caaactggca atatcattct
     2821 atcgatcatc caagcatttg acggagaatg gctaagtgtt ggtaaaaaag tattgtctac
     2881 ccttttcttt ttcttaggga ttcttctaac aaagtatcta attgattatt ttagaaaaaa
     2941 agaaaaacat ttctggcgct tgtttgttct atattatgaa gcagtgattt ttttctttgt
     3001 tagtttgaca cccgtccatg agcatcccgc attagtaacg attttgattg cttttactgc
     3061 atcgatccag tgggtagctt ttgataaaat caacgggcgt gcctacacta atttatttac
     3121 aactggaaat ttaaaagggg tagcaacgaa cttatatgat tattgtattt ctaaagaaaa
     3181 agcagatttt gatcgatttt tccactatct aagtgtcgtt cttgccttta tcgcaggagc
     3241 tattgtcctt gttttctgtc atcatctgtt tggcgcccat tcgattttga tcgtttccat
     3301 tttatttttg atcatggccc tcactcagac atttcattta tggcaatttt atcatacgaa
     3361 tcggttcact atttgatctc aatgaatcaa aaaaactaag caagatgctg ctcacagaga
     3421 aatatctgcg agtgagcaat ttgcttagtt tttatattat ttagttgtaa agattccctt
     3481 tgctttcggg ttatttgtag tgtagcggat cgactgggca tctgcctctt ctgtcatttt
     3541 ctttgcccgc atcttgattt cttgtacgat ttgttctcca catgccggat cggctacgaa
     3601 atcatatttg tccccatcga tcactatttt gggacatata tcaaactgtt cataccactc
     3661 ttcgtatcgt cgattgagtt ctttatagta ggaatagagt tcaggatctt gttctagctg
     3721 ttcatattcc cggcctcttt ttttgattct cgctaacata gtttcaaagg atacacgaat
     3781 ataaaccagt agatctggtt tcttttgtgg cgaaatttct tctaactcct tcagcatcgt
     3841 ttctaaaaga ccttcatact gctttacttc tacttcagaa acacgcccta aatctgcatt
     3901 caaatgaaac agcatactat cttcgtagat ggagcggtct agtacgttgt catcatggct
     3961 gaaagcatct ttgattgcca aaaagcgttt gttcaaaaag aaaatctgta ataaaaaagt
     4021 atacttttct ggattagaat aaaatagcgg caatacttca ttatcttcca cgttctcata
     4081 aaaaggtttt gtttctaaat cttgggcaag catttcagtt agtgtgcttt tccctgcacc
     4141 gatcgttcct gcaacaatga tcacaattat cggcatcctt cctgtaataa aatctgttct
     4201 tcattttact acttcattta tagaaataaa agccttgact ttatctgctc cttaaaagct
     4261 acttctctaa aagtagccaa aagtaactcc tattcatgaa aataaaggct gtgacaaaag
     4321 tcttgtcgca gccctttttt caaataaaca gtattctcct gccaacacct cggtcacaag
     4381 gcaatattca taaaacatga aaagctgttt tttaacatta ttccttgtaa ctcagagcta
     4441 aacgggagac tcacaacctc tttatgctct caacatttta tctgttttta ttcttctatt
     4501 gtacttgatt tgcgtacaac taactgccag tgcagattat gagcggtatc attgtcttct
     4561 actgcgcatt tcatgacacg tatcatcaca ctattttcat agattttttc tatttttcct
     4621 tttattggtg attcaaataa atcagattta aatgcatatg tttcgccgac ttcaaacaat
     4681 tgttcatttt ccataagaat ccctccatca ccttttataa ggtcagtaat tttttaacat
     4741 aaatcactcg aaaaagcaca aacttgtcat aaaaagtact tttcgtatca aaactgtcac
     4801 tttttttggt aaggttcttg gaaaacgaag atttttttag gagatcatga cggttttgat
     4861 gtcaaaacca ctttttatga aacaaaagac gttttttatt ttttttagca taaaatttgt
     4921 ggtggaggta cgtatgagat gaaagataaa gttgtgaaca aattattaga agccttattc
     4981 agcggagaca aatcggaaga agatatggtg tatgtccaag taaaatttgc cttagaagtt
     5041 ttattgaata atcttggaaa actagcggtc gttctagtct tttctctggt gacaggctca
     5101 tgggcggaaa caggaatcac ctttctttct tatatctgca tccgcagata tgcttatgga
     5161 ttgcattccg actctgaatt cgtttgtctg ctgtggacac tactttattt atggggcgtt
     5221 cccttggtga tgaaacatct gcaattgact atctccttcc caatgatggt ctttttgtta
     5281 atcagctgct ttttgttact gcttcgctat ggatcaagag gtacagcaat caatcccatt
     5341 gaacccgaga aacgtccccc attattaaag aaggcgatca gcatgtttct aattttttct
     5401 ctcattactt tatttttctc agcttcttac ttttctacct atttgctact tgggattgtt
     5461 ttagaaatag ctacgttgct tcctattaca aactacttga tgaattttgg aggaatcttt
     5521 catgaaaaaa acaatcatta aacaattaac taacttatct gacttttttg tatctggctg
     5581 ctgctttggt atgagttctg aattcaaaat ccctgaaaaa ttaaaaaaat aatacgctaa
     5641 ttgtgctata atttggtaaa aatcaatggg agctgtactt tatgttttta tggtccatag
     5701 cgttgcaata tttgatttac acaatttgta tcttattgat tgaccatcac atccgataca
     5761 aatatctgtt tgcgggagtc attctgattt cttgtatttt gattggatgg gtctattcga
     5821 ttttagggat ttttactgct ctaattgcta taggaatgct gatgcttact tttttcttct
     5881 tttcgacttc gcgtcaacaa ttggtttacg cgcttccttt tgggttattg acgtcgcttt
     5941 taggagatca cttaacaagc atcgacgatt tttttgtttt gaaatcttcg gttattgaac
     6001 caggagattc attgcagtat tttcacattg tcttatccgc ttttttatct gctttattag
     6061 ctttttgttt aaaaaaatcg ttagagactt ggtttcacac tgtggaccgc cgtatgatcg
     6121 gtacgatcgg gacattgata ttatttacat actatatcat tattttttat acccgttttt
     6181 ctggtgaaac acctgatgtc ttagctttga atacgagttt tttcattcta tatctgtgtg
     6241 ttggcctgat catcttggtt tattattgga aaatctctaa taagaaatta gctgatcagt
     6301 tattagagca acaagtcagg atgcaacagg attatatcaa agatctagaa aaaaactatc
     6361 aagacttgag agaattcaaa catgattacc aaaatctgct tttttctctt cacagctata
     6421 tttcagaagg cgacttagaa ggattacggt cttattatga tcaaacgata ttacctacac
     6481 gccagatgat gaaccttttt ccggccaatc taagtatgtt ggataatatg aaagttcctg
     6541 agatccgtag tttactgtca ttaaaactca tgatggcaca ggaaaaaggg ttgatcgtcc
     6601 agcttcattt tcctgagcca atcgaactgg atacgaaaca tacggtaaac cttgtacgta
     6661 tgcttggtat tatcttggac aatgcgattg aaggagctag tgctgcaaag aagaaaaaaa
     6721 tcgaattgac catactcaag aagaatcatt ccgtgatgat tcgtgtgatc aatacaacaa
     6781 caaatacatt accgttaaac cagttgaaac aaaaagggtt ctcaacaaaa acaaatccaa
     6841 aaaacgaagg ccttggcttg tttatcttgg atgaattgac aaagaccaat ccttatcttt
     6901 tccttgaaac atcaattgaa aaccagcgtt tttctcaaac gatttatata caaataaaat
     6961 aaaggagtaa tgatatgatt cccatttata tttgtgaaga cgatccaaaa caattagaag
     7021 ttttatcaac ggtgatcaaa aatagaatca tgattgaaaa cttagacagt tatgtccata
     7081 tttctacctc tgatccggaa gaacttctca atgcagccaa gatcagaact tcttcttttg
     7141 gtatttactt tttagatatc gaactagaag acagtgaaat ggatggtgta gcgatcggta
     7201 aattgatccg tgaattagat cctttaggaa aaataatcta tgtgacttcc catacggaaa
     7261 tgtcgatgaa aatcttaaaa agcaacatcg aaccgactga ttatatcgtc aaagaagatc
     7321 tttatgattt aaaagaaaat gtcgagcata tcctgagcaa aattttcgaa gattttcaaa
     7381 tgtcccaaat ccaaaaagat atcttcaaaa tagagtttaa tgatgagatc aaatttttac
     7441 cgatcaaaga catccaatat tttttctact tcgctaggag cgccacataa actggaagtc
     7501 catttgactc atgcccaatt acaattctat gagaagatca aagaaatcga gaaaatgcat
     7561 cggaatttta tccgttgtca taaatcctat gttatcaata cacaaaatgt gcggtcgatt
     7621 aataaaaaaa ccagagaagt cacgttggca aacggcgaga ccatcccagc ctctatccgt
     7681 ggattaaaga aattgatttc ataaaaagaa ccgaatagtt tggaccgact aaatgaccag
     7741 ccgatccaaa cccttcggtt cttttttgcc tatcagtgta aaagcatttc ctgtgcaatt
     7801 tcacgtccat ccattgtatc tggataaaat gttggccaat tctccatttc tttcagcaat
     7861 acttcttgac tttcatttcc tgcatataaa tgaaaatgtt cggagtcagc cggtgcaatc
     7921 gtgtgatcac tgaattgaat atatctaaaa gcgccacttt ccttatcttc tgcttcgaat
     7981 agatacctta cgccacggtt tcctgcctcg taggtcaaga tttctttccc tacataacga
     8041 taatttgaac gtttcgttgt tccatcctta aacgtaaatg ccatctcttt ctcactgatc
     8101 ttgattgaag caatatctgt ttgatagccc ttcgtataat attctttata ttcttgtgct
     8161 gtcttatcct gattcaattt ttctttatat tcaaaaacag gatcaaaagt cccatcttct
     8221 acatacgtat ctactgtttg ccaagttcct gcccaatctg aaagagagcg atcttgtaca
     8281 tcctcgtctt caaaataacc gttgtagacg ctcttttctt ttttgggggt caaagtatct
     8341 tctagcgggt tttctgtctc tgtcgttttt ttcaacgctt cgaggtttgc ttccatgata
     8401 gaaagatagt tttctccatt ttctatttgt tcattcgtca atccttccaa aggattcaat
     8461 acttctaaag atacacctgc ttcgtttgct aacgttctgg caatactatc tttggcgttt
     8521 tcttcaaaat aaatatagtt gattccatgt tctttaacaa agtgtttcag ttcagcaatt
     8581 ctcgccgcag acggttcttc actagaagaa agaccagcaa tcgatacttg attcaatcca
     8641 tattctaacg ctagatagtt gaatgcagca tgttgcgtga caaaagatct ttgttttgct
     8701 tgcttaaagg tttcttgata aagctgatgg agtgcttgaa gtttctcggt ataggctgct
     8761 gcattttttg tccatacttc ctgtttttct gggtaagctt caatcagctg atcacgtatg
     8821 tttgctacct gcttgattgc taaacttgga gctagccata catgtgggtc tagctcatgt
     8881 gtatggcctt cttccccgtg ttcatggtca tgatcatgtc cttcttcact tcccggaagt
     8941 aaaagcatac cttctgtcgc ttttatcgta tggacattgc cttcttgtaa tgttttttgg
     9001 attgcgggga cccatgtttc catgttttca tcattataaa caaagatatc tgtatcttgg
     9061 atctttgcca tgtctttagc agaaggttca taatcgtggg aatcagtccc tgctggaatc
     9121 aataactcta cttctccttc atccccgacg attgcttttg taaaatcata catgggataa
     9181 aaggtcgtca tcacgctcaa tttttcttta ctgtcctcgg ctttcccact tgccccgcaa
     9241 gcagttaaca tacctgctaa aaccagcatg gccaatatac ctatcaatcg tttcatttat
     9301 gacactcctc ttctcatctt ctttcatttg aatgttccga ttcgcttttc gcaaaccgta
     9361 atagttacgt tttagcaaca ggatttaatt tatcatatgg ttaaaagaac tgtcaagcgt
     9421 tttcttattt taattgatga gaaagtttat atgaatgatt gacgatattt ttgcaatcaa
     9481 cctctatcat tttcctttta tacccgcttt tgtctataat gaagttatag ataaaggagg
     9541 aaaaagagtg aaaaaagaaa cagatacaac gaatcgtttg atccgctttt taggaggaaa
     9601 aacctcttac tatgttttag gaatagttat cttacttgcg ctgatcattt attttttcca
     9661 tcagatctcg tttatattca agcctgtcgg aatcattatc gctactatct tgcccccatt
     9721 attgtttgca ctgatcttgt attatctttt ggaaccagtc atcaaacgtt tgagcaagca
     9781 cctttcgagg aacttgtcag tcattatcgt ttatgtgatt attttatttt tattggggct
     9841 tggaagcata tggcttattc cttttcttga agaacaggca aagacattga tcagtagttt
     9901 gccagatttg tttcaagatt ttcaagagag tctccgacaa tttttggaga aaacaccttt
     9961 tgccgattca tttaatcaat tttttgcttc tattgacgac gtgacgtctg atatagctgg
    10021 atttattgga aattattggg aaagtggcgc acaacggatc agcaacattt tttcaactgt
    10081 tacagctatt tttattactt tatttactgg tccaattgtc gcttttttcc ttttgagaga
    10141 tcctcaaaaa ttttatcagt cagttttagc gattgtccca ccagcatttc gtacagattt
    10201 taaaaatttg acaaagattg ctaatcaaca attgggttca tttctaaagg ggcaaatcat
    10261 tgcttcattt atccttggag cagtctactg ggtttgtttc ttactgatcg gattagaatt
    10321 tgctagtgtt ttagctattt ctgctgggtt gttatgtatc attccatata ttgggccgtt
    10381 tatcgccttc tttccaggtt tgatcattgc attccaagac tctactttta tggctgtcaa
    10441 atttgtcatt gtctggtttg ccgtacaact tctccacggt gacttggtga ttcctcgagt
    10501 aatgggtgat cgtttacaga tccatccgat tacgatctta atcgttttac ttgtcatggg
    10561 tgatctaatg ggaattgtgg gcgttatttt tggtattcct atctatacgt tagtcaaatt
    10621 actcgttact tttattttcc gtaaattcaa acaacgctac aataaatttt atggcgataa
    10681 aggagagtat gaaaactctt cattttctga agaagattat tttaaataaa acacaaaacg
    10741 tcgatcttac gagaatagct cgtaggaacg acgttttgtg tttttgtttt aatttttcag
    10801 caaagcaatt gcctggtatg gttttagctc cagactttga tgaagaacag tatcggaata
    10861 attatcgata agaatattgg cttctgtgaa tccttctggg atggcgatcc ttgtatcttt
    10921 atcatagaaa tttgttaata caagcagctg ctgtccttca aattggcgaa gaaagccata
    10981 aacttccgga tgatttgctt cataagcatg ataatctcct tgtgcaacga ttggatatgt
    11041 ttttctcagc cgaatcaatt tcttgtaata ggacaaaata gaatcttcag aagatagttc
    11101 tttttctaca ttaatctctt ctagcttgcc tccaactttt aaccaaggtt tccctgtagt
    11161 aaatcctgct tgttctgtgt ctgaccattg catgggtgtt cgagagttat cgcgagattt
    11221 tgctttgatc cgacgaaacg cttctttttc tgtgaatcct tgttcaagga gtaacttata
    11281 agcattcaaa ctttctacgt caatatagtc ggaaatcgat gaatagtctg ggtcgatcat
    11341 gccgatttct tctcccatat aaatataggg cgtcccacgg ttcaaatgga tcatggttgc
    11401 tagcatctct gctccttgtt ttcgatactg atcgatagct acaaatcgat tgagcgcacg
    11461 tggctgatca tgattattaa aaaatagagc attccagccg ttaccctcac tcatcttttc
    11521 tccccatgta tgaaatagtc gctttaattc tgcaaaatca aatggaggag tagaccattt
    11581 attcccattt tcataatcga ctttcaaatg atggaaatta aagaccattg ataattcttc
    11641 tctactaggt tctgtataca agatacaatt ttcgatcgtc gtagagctca tttcaccgac
    11701 tgtgatgata gtggaatccg tcccaaacgt tgcttggttc aattctttta gatacgtatg
    11761 ggtgattggt ttgtccgtat attcaggttt gccatcattt tctttattat ttttcaattg
    11821 ttcgtcttta ccgataacat tgataacatc gaaacggaat ccttttaccc ctttttccat
    11881 ccaaaaacga acaactttga atagttcctc tcttactgct tggttccgcc agttcaagtc
    11941 agcttgtgtc cgatcatata agtgcaaata atatttcccc gtatctccaa atggcgacca
    12001 agcacttcca ccaaattttg atacccaatc cgttggctct tctcttaaaa tgaagaagtc
    12061 ctgataatgc tggtcccctg ctagtgcttt tttgaaccat tcatgttctg tcgaaacgtg
    12121 attcagcacc atatcgagca tgatttctat ccggtatttc tttgcttcgg ctattagctg
    12181 ttcgaattcc tccatcgtac caaagatagg atcgatggct gtgtaatcag agatatcata
    12241 accgttgtct ttttgagggc ttggataaat agggtttagc caaatcatgt cgatgcctag
    12301 atcattcaga tacgggagtt tttctttgat tccttttaaa tctccaattc catcaccatt
    12361 actgtctttg taagatttgg gatagatttg gtaaatgact ttgtctttaa atgtcatgga
    12421 tatttacctc cattcaactg atttattacc taccacaact tatatataca agttatcatt
    12481 tattattata actgaaccga aagcgtttgt aaaatttatc tttatacatt ctaagaggtc
    12541 tgttgcccaa taaaaaacaa gagatcagac atgtatttgt ctatcctctt gtttaaaata
    12601 gcaaaatatt cttgtttctt ctataaatgg tgttcaaaag cttttatcta cggcaatcag
    12661 tcttctcaaa tgtaaaacat gaggagctaa ttttatttga gtattctttt tgctcgatgc
    12721 aggacggctt ttttacaacc tctgattcat ggtgttcaat tagcagctga ccctcgttat
    12781 tagctcaaaa cgctgatttc acaacctctg tttttattca ttcgatttca acgcatcaat
    12841 catatcgata tgctttaatt tgaaatgggt aacaatcatc acaataaccg taaatacgat
    12901 agttaatcct gctgataaga cataagccca ccatgtgatc actaatggaa agatgacatt
    12961 ctccatagac gcttgttgca agatgaagtc agtcaatata tatcctacac caaatcctcc
    13021 gatgattcct aaaagagtaa agatgatatt ttctcgaaca atgtacatcg tgacttcttt
    13081 atcaaaaaat ccaaggactt ttattgtaga taattcgcgt acacgctcgg aaatattgat
    13141 attagttaga ttatacaaga cgataaaagc cagcagtcct gataatacga caaagattaa
    13201 gacaaccgag tctaagttcg ccattgattc ttcttgcgtt tcgatttgtt tggacatgaa
    13261 agacgtattt gtcacagcgt ctgtggcaag cagtttctcg gaaagcattt cttcttcttt
    13321 tgttgtcatc ttatctgtac gcagcaaaaa tgcattatcc atcatcgatt gatcagtaac
    13381 tgaagcataa tattcagggg acatataaat aaagctacct agatagtttt tcacgatttc
    13441 ttttacttcg actttccatt ctttttgatc ttcatcataa agcgaaatat gatctcctgt
    13501 agataagtca aatagttctg ctactttttg cgtaatagcg attcctgttt taggcaagga
    13561 gcgtgggttc tcttcaagat cagttaaatg gataaaacga tccaaatcag ctgttttact
    13621 tggtacgatc actgtcagac tttgcgaagc atgtcctttt tttctcaatt ctacggtttg
    13681 agtatgaata gctaggatct catttacttt ttcttgcttc tgcagcatat ccgctgcttt
    13741 ttgacgacta tcttccgtgg atgaatccaa tgtaacgatt gcttgatagt caatgatcgg
    13801 tccaaattgt ttggctgaaa cagaacttag agaatctttc aatcccacgc ctgcaaccat
    13861 cagcccggca catccagcaa ttccgataat cgccataagc attcttgact tatatcggaa
    13921 gagatttcgg taactgattt tttgattaaa gctaaggcgt gaccacagcg gagtgatgta
    13981 ttcaagaaaa atgcgtttcc ctggcttcgg tgctttcggt tgaagcaatg cagcaggccg
    14041 ttctcttaaa tctttgaata aaacgatcat tgcagcgcca aatgcggcaa tgaagaatgc
    14101 ggcagctgct tgtaaaatcg gcggccagtc ataaaaaata agcgcactcc caatatcata
    14161 ccgctcgtta gacagctcaa aaatgatcct tggcaaaaga ttcgttccta atatcgttcc
    14221 taaaacaata cctatccctg aggctagact tgcatagatt gcatattttt ttgcgatttc
    14281 aactttcgta taacctaacg cctttaatgt cccgatttcc ctgcggtttt cttctaccat
    14341 acgtgtcatt gtcgtaaagg tgatcaatgc ggcaataaag aaaaagaaaa caggaaaaac
    14401 attggcaatc gcagcgatac gatcagacag atcaccaaat tcttgaaaac cgacattttc
    14461 tgaacgttca ttgtataggt atgatggttt agttaaattt tcaatttttt ctgcatttcg
    14521 attcaattct tcctctgcag aggccagttc acgttctttc tcaactaatt gcctttcttg
    14581 ttcagcaagt tgagcttctt gttgagataa tgtttgaaca gcgattgttc gttgcgcttc
    14641 aggaagctgc tggatagttg ctgactgttg cgctaactgt tttttagcat cttctaaccg
    14701 tgttcttgct tgcgttgttt gtgctttgcc atccgctatt ttttgctgtg ccggttctaa
    14761 ttcttcgttg gccgattttt tcagctcttc taagcgttct tcgggtcgat cttgtaaata
    14821 cttttctgcc ttttcttgat tttgctccat tttatctttg taagctttgc tgtacgtatt
    14881 caaattcttt acgtttgtaa aatcaagata taagacagaa taaatatctg ttttaaaaac
    14941 ggattcaggt aaatagacaa aataatcgac gctaccattt ccgacgttcg cgtatcctct
    15001 ctccaaattg ttgatgaaca atggagaacg gacaaagccc gtgatggtat actctttttc
    15061 tttcaattca tctgaatcaa tctgataaat atcaccgatt ttataccctt catcttccgc
    15121 tttttcatcc aatacaagct ggtttggttt ttccgggagt tttccatcag ttaattgtag
    15181 cttgttctgc ttgttatttt tgttataaga aaatatttga atcacttcgt ttttgccagt
    15241 attggaataa tagatctgtt ttcctgtttc tacttttgca tctgggatct tttctgcttg
    15301 agcaatatct ttttgtgtca agccaccagt agaaacgatt tgcaaatcag aaagtcctgc
    15361 tgtaccaaca tagtgatcca tcgttttttg catgacaggt ccagcagttt tgacacctac
    15421 aaataaaagt gttcctagca tgacaataag gatgattgca atgaatcgtc ctttagattg
    15481 ccgaatttcg cgaaaacctg ctttaagata tgttcttgtt ttcataaaat ccccctacca
    15541 tacaatctca tcaatggatg aaggatgatc atttaattca acagacctta ctttagcatc
    15601 attaatgtga atgactctat cagcgatggg cgctaaagca gaattatgtg taatgatcaa
    15661 aacggtattg ccgtgttgtc tgctagcgtt ttgcaataat ttcaatactt gcttgcccgt
    15721 ttcaaaatcc agcgcgcctg tcggttcatc acataaaaga agtttgggat ttttagctag
    15781 tgcacgagca attgaaaccc tctgctgttc gcctcctgat aattgggacg gaaagttatt
    15841 caatcgatga gctaggccca cttgcctcaa tacctctaca ggatcaagcg catcaggaga
    15901 aacttccgtt gccaattcca cattttcttt tgctgttaga ttaggaacga gattgtagaa
    15961 ttggaaaacg aaccctacat ctgttcgtcg gtaagcagta agctgtttat ctgaaaattg
    16021 cgcaatatct gtgtcatcaa taatgatctg accttcatca ggtgtatcca tcccacctaa
    16081 aatattaaga atcgttgact ttcctgctcc acttggacca agtataatca ctaattcgcc
    16141 tttttcaatt gaaaaagaaa tatcgtcatt tgctgtaatg gtcgtttctc ccattttgta
    16201 tcgtttgtat tcatttttta cttcgacata cttcactctg attcctccat tttcacgata
    16261 tttctcgcta tatctatgca tcgaatgcgt tttctcacac ttatttattt taccactgaa
    16321 tttctctaga ctaaagaaag gaatccttta atttagttga caaataaacg atttatgatt
    16381 tacttacata aaaaacattg ttatttgaaa gaaggaacat catgaatgat ttaaaggaaa
    16441 atatgatttt tgatttatca aaaactgctt ttgtcgcgat cgacatgcaa aaagagatcg
    16501 taaataacgg tacactctct ccacacactg cagcatctat tctgacaaac aatgatttgt
    16561 tagtaagaac attgaaacgt accgctgcct tgaaagtttt agtgaacgta gacatctcca
    16621 ctttccctta tttgagtcaa caaactgata tgggtggaat gactgcttct gtacctccag
    16681 aattcactga tttacttttg aaggattctt tgaaagatac agataatatg ttgactatca
    16741 caaaatacaa tccaagtgct ttttttggta cttctcttga tcttcaactt cgccggcgtg
    16801 gaatcgaaac tatcatttta agcggtgtag cgacaacaaa cggtgtatat gctactgcat
    16861 tagatgcctt tcaacacggg taccatatcg tgcttgctga agatgcttgc agtgatcgtg
    16921 acaaagaatc ccatcaattg ttcataaaaa agatatttcc gaaaactgca cgagtacgct
    16981 caacgaaaca aataatcgaa gctatccaac aaagcaaata agtactggca cacgtgattt
    17041 tcatttactt ctttttgatt tcatgggaaa ataaccacag gatttagata ggagtatgaa
    17101 catggtgaaa aaaaagatca caatcaaaga agtcgctaaa ctttccggcg tttcaatcac
    17161 aactgtctcc ttgatattaa atggaaatac ggagaaattc agtccgaaaa ctgttcagaa
    17221 agttttagct gccaaagaag aattaggcta tcagcctgac tattttgcta gacaaatgat
    17281 taccaaggaa acgaaaacaa ttggtgtact cgttcctgat atcacgaatc cattcttcag
    17341 tacactgatg cgaggaattg aggatatctt gtataagcag aatttcgtca cgatattatg
    17401 taatgctgat tctgatcatc aaaaagaaat cgaatatcta gccgagctca cacgtcgagg
    17461 tgtggatgga ttcattatcg cgacttctgc tgtctctaca gatgcaatca acgaaaattt
    17521 gaaaaaacaa ggccggccct tcattgtttt ggatcagaaa aaatctgaag gatttagtga
    17581 tgccgtacga acagacgatt ttcgcggtgg ttacctagcg ggcatgcact tgcttagtct
    17641 aggacatcaa acgatcgccc ttgtctatcc tgaaaatcca ccggaaaatg tccacgctcg
    17701 gattgagggg tttaaatctg ctcttgatgt ttaccaaatc cctcacgacc aactgatcct
    17761 cttacctaca cagttctcaa aacaaggtgg ctaccagatc acagcagaac ttcttgattc
    17821 tgctgcaact ggtgtgtttg ctttaaatga tgaattagct tttggattat atcgcggatt
    17881 agaggaagct ggaaaaagca tccctgaaga ctacagcatt attggctatg ataatataga
    17941 tatgtgcgag tatatcaagc cgaaactgac aacgatcgca caaccaatct tcgaattggg
    18001 acaaacatct gccaagttat tgttagatcg gatccaattc ccagaaaaag aatgggaaga
    18061 aaaaagatta cctgtacgat tagaaaaaag attttcaact gcacccttga aatagctttc
    18121 aaggtgtgct atatttttat cgaactatta aaacgtttta ctaaaacgtt ttaaaaaata
    18181 attttcccat caaaataaat gagatagttg gaggtttgta aaatgaacac ggttacagta
    18241 attggaagta ttaatatgga cactaccctt cgtttaacca acatgcccaa gccaggcgaa
    18301 acgatgcatg cgcatgagat ttttcacgct ggcggaggta aaggtgccaa ccaagcagtg
    18361 gcagccaaac gttcaggcgc acggacatct tttattggag gcgttggggc tgacagcgaa
    18421 ggacaacagt tattagatct actgacaaaa gaaaacatcg atacatctgg gatagctgaa
    18481 atccaaggcg ccacaaccgg acaagcaatg attatggtag acgcagcagg agaaaatagt
    18541 atcttgatcc atgcaggggc aaataatgct ttccatgaac aagaagtcct aaaaaacaag
    18601 caactgatta caaacagtga ctttattatt gctcagtttg aaagtagctt agatgcgacg
    18661 atcctagctt tttctattgc aaaagacgca ggaaaaacaa cgatcctgaa tcctgcacca
    18721 gctcgtgaaa cgattcctac tgaactactt gaaaagacag atatcattat accaaatgaa
    18781 acagaaacag aaatcattac aggaatcaga gtgacagatc ataacagttt ggtcgcagca
    18841 gccgaaaaac tccatgaatt aggtattgga acagtaatca ttacactagg cagtgccggc
    18901 gctttctatc atacagaaaa agaacacggt atcgtccctg cttttaaagt ggatgccgtt
    18961 gacacaacag ccgcaggtga tacatttatt ggcgcattaa gcagcacact tcagccagac
    19021 cttagtaact tgaaagaagc gatcctttat ggaaatctag cctcttctgt tgctgtgcag
    19081 tcttatggtg cgcaaccttc tatcccttac cgcgaagctt tagaacaatc agcagtctaa
    19141 aattaacaat gagaggaaga aacaaacatg aaaaaaggaa aagtaatcaa ttcagatatt
    19201 tcacgagtaa tcgcacaaat ggggcacttt gataaattaa gtatcgggga tgcaggcatg
    19261 cctgtcccct ttggtactga aaaaatagat ttggctgtct caaacggtct tccaagtttt
    19321 ttatctgttt tagaaaacgt tttagaagaa cttgaggtac aatcgattga attggcggaa
    19381 gaaataaaga caatgaaccc ggaaatattg gaaaaaatca aattattgtt accagataca
    19441 ccgattagct ttatcccaca tgaagaaatg aaaaaagagt tgaataactg tcatgcattt
    19501 attcgtacag gcgaaatgac accttacgcc aatattatat tagtgagtgg agtggtcttc
    19561 taatggaaac gacaatgaat cctgaacaga tggtactgtc cgagcagatt catatgctga
    19621 ttgaacagtt gggaggaaaa gacaatattg attctgtcct tccatgtgcg actcggcttc
    19681 gtatcaaatt gaaaaaacag ccagtagcca atgcagaaaa attaaaacag caaatccctt
    19741 ccattaagta tttcatttta ctcgatagat atgcagaaat aatcatggat cagggattgg
    19801 ctcctgctta caaggaattg acgcgtcaaa tcgatcattc agctccgaaa gaaaagctaa
    19861 atccgatcga aaaactattt ggtttgatat cggaaatctt tacgccgatc attccgctgt
    19921 ttgccggttc tggtatatta aaaggtctgg ttgttttagc gactacgatt ggctggctta
    19981 gtgaaacaag cggaacttat catattttgt ctgctgcgtc gaatgccgtc ttttattttc
    20041 tgccaatctt tcttgctttt tctgctgcca aaacgtttaa agtgaatgga tatattgcgg
    20101 ctgtcattgc tgcagcactg attgaaccga atctgacctc tttactttca gacagtggca
    20161 aagcagctac tgattttctg gggattcctg tagtactgat gcagtatacg tctactgtca
    20221 tgccggcgat tttaggtatt ttcttttatt cttttgttga aaaattcctc aaaaaatata
    20281 ttcgcgaaaa tatgcaatta gtatttgtac ctttactttc attagttatc acggttcctt
    20341 tgacgttaat ggtattcggg cctgcgggtg tttaccttgg cgaaggttta gctgccgcaa
    20401 ttacttggat gatggatatt aacggaccga tcagtggcgc aatcatcgct ggtggttgga
    20461 atattttggt aatatttggt atccaatggg ctgtaaaccc tgtcatgatc agtaatatct
    20521 ctgcttatgg gttcgaccga atcgttccgt tgacaggagc agccaatttt ggaatggctg
    20581 gtgcagcttt aggtgtattt ctgcgaagta aacgttcaaa aacccgttcg atctccggtt
    20641 ctgcttttgc ttcgatttta cttgcgggag tgaccgaacc aactgtttac ggaattgcga
    20701 tcccattgaa aaaaccattt gttgctgctt gcattggtgc agctgctggt ggagctgtaa
    20761 tgggatttgc ccaagtgaaa gcgatcgctt ttgtatttgg tagtctgacc acattgcctg
    20821 cattcatttc cggtactttt ttctggtact tagcaggact ggctgttagt ctagtcgtag
    20881 caatgatcac tactctcgtt agcgggtttg atgaagacct aatgtcttat gaatagttaa
    20941 tgaaaaagac tgaagtatga gatgtttatc acatatttca gtctttttct ttttaggatc
    21001 attattttta taaatttttg tgatactttc caaactttgg tataatttac atatgcataa
    21061 ccatttttat ccccacttgt ttatccccaa acaaaggtgg tctatgcaat aaaatgaacg
    21121 tcaaagaaaa acgaatgaat gctcgttgct tatttacaaa ttgttttatt tagaatacac
    21181 agacagattt atgaaaagaa aaagcttttc gacacgatta tctgttccaa aaaatgtcta
    21241 atgaatgctg aataagacag aaaatcaatg aaaggaaaag gactagttta ttatgaaaaa
    21301 taaaaaaacg atacgtttgc tagctgctac ttggctgatt gctccgtctg ttttgtcgac
    21361 caataccgta tttgccgtag ataatcaaat agctgatcaa acagtcacaa cagatacgac
    21421 tacatccagc tcagctacta gtgaaacaac ggattctttt acagaagacc caactggaac
    21481 aactaactca acaacaggat cgaccgagcc aactgaaatt gatccggctg atccatcaga
    21541 aaatgaaaag ccagccgaga catattccct tacaattgct ccttctttga ttggtaagat
    21601 ccaactgact gcttcgacag cggattcccc tgaacagcaa cctttggata ttacggaaag
    21661 tggaacggta gaaggtttat tagcggaaac gaaggtaact tacactatta cgccagctga
    21721 aggtgaacaa attgcttcag tcgctgttaa tggtgccaaa gaagtaaatt atgatgcaaa
    21781 gacctttaca gtaggtacga acaatttatc tattactgca gaatttaaag ctggaccgga
    21841 tacttcaaca gagaaaccaa ctgatcctag tgaagaagtc cctagtacac cagatccgga
    21901 aacgccaaac aaaccagatg tgggtaataa caacaataat aatggttcaa ataattcaaa
    21961 caataataac aataattcga acagcgggaa ttcttcgaat caaacaaacc catctggatc
    22021 gaataacaat ggaaatactc agagagaacc aaaccatcca aacaatggtt catcgaatgg
    22081 cggaacgaac aatgcttcat ctcgtccaaa tacagcaaat caaagcggta tagagaatcc
    22141 atcttctgct tcttctgatt ttgttgtaaa atcaccaatt gatgctgtat tgcctacaaa
    22201 tacaacgaat gtccaacaag caatcgttcg agaagcattc aagcatcttg gaaaaccata
    22261 tgtatgggga gcaaaaggtc caaatacttt tgactgttct ggtttgactt attacgtata
    22321 tatgaaagca accggtcatt atatcggtgg ctggacaggc gaacagcaat atgcaggtac
    22381 gcagattcca gtaagtcagg cccaacctgg cgatctcgtc ttctggggac cttcttccgg
    22441 tgttactcac catgtaggta tctatattgg gaacggacaa tttatccatg cgcctcaacc
    22501 aggagataaa gttcgagtaa cttctatttc agatttcacg cctgattttg ctgtccgagt
    22561 aaacttagca ggtttacctg ctgcttcagg ttcgctgaca ggcggctcat ctattttaga
    22621 tggattagat ggctcgttcc atttcagtca aaaccaaaca actgatcagt tcttgaaaaa
    22681 gattgcagat gatgcacagg aaatcgggca aaaagaagga atctacgcat cagtcatgat
    22741 ggctcaagct atccttgaaa gtggatctgg aaacagctta ttatcaagcg aaccaaatca
    22801 taaccttttt ggtatcaaag gatcatataa aggaagcagc gtgaccttca atacacttga
    22861 acaagatagt tccggacaaa gctaccaaat ccgcgcacaa ttccgtaaat atccttctta
    22921 caaggaaagt ttggaagact atgccgactt gatcaaaaat ggactgactg gcaatcctga
    22981 tttctacaaa ccaacttgga agtcggaaac aaaagactac aaagaagcaa ccaaatattt
    23041 ggaaggacgt tatgctacag atcgccaata ttcacaaaaa ttgaacgcga tcatcgaagc
    23101 gtacgacctg acaaaatatg atgaaccgaa aaaagaagca gcaacaactg aagaagcaaa
    23161 cgctgaaatc ctcaatatgt ctaagcgatt cgatgtgcca atcaaatgga caacagatgg
    23221 cgtgccaatc acaggattag aattttcaac gataaaacgc gtcaacaacg cttcttccat
    23281 cttccgtgtc gcttcaatct gggatttgtg gaacaatttt acggcaagac agataccaga
    23341 aacaactact cgtacagtta aagctgcaac tgttcctgta ctaaccttgt taagtttaga
    23401 caaatcattc tggcttgatc tgcctttaaa gaaatagaat aaattaaaaa ggggatgtga
    23461 cactgtcaca ctcccttttt aatttattct atttctttat atttattttg tattccgtta
    23521 caaactcgcg tacttggtta ggttccagag tgacatctcc aaaactttca agtcgctccg
    23581 ctcctggcag ttcttgagtc tcaaatgtga taccgccatg atttactaat ttattcccgc
    23641 gcatttccgg tccatcttct ccaaaatttg ctgtaaaaat cacgattcct gaagcatctg
    23701 tttttacttg gatcgatact ttttcatctg ggcttgtcaa ttccgcagca tttagtaaac
    23761 cagattgttt tttcaacaaa aatggatgat cgatgccgcc cactatcatt gattggcgaa
    23821 aatcactggc aaagacctca cctaattttt taggacgttg gaagtcaaat gctgtgccac
    23881 ttactgaaac tacttctcct gtaggcgttg tatctttatt gagcgtagca aataatcggc
    23941 tattcaacca aagtatatgt tcgtcgattg actgtgctgg atcaccggtt aaattgaaat
    24001 agacatgatt tgtaggattg aatactgttt tgcggtcact gatcccttgg accctcactc
    24061 gccatgtatc atcgttcgtc agcgtatacc gtacttctac atctaaagtt ccaggaaatc
    24121 cgttttccat atctgggcta gtctgataga aaatgaccga tgcttcgttt tcaccattga
    24181 tgactgtata atgccactgc ttggcttcaa agctaggtgc accaccatgt aaacaatgac
    24241 cagtgtccgg gtctgttttt acttcgaacg tttcatcatc taatttgaat gtgccattcg
    24301 taatccgtcc agccatacgt ccgattgaag caccgatata agggtctttt tctagatatt
    24361 cttttgcttc atcaaaacct aagatcaact ctcgtttttc attatcgatt attgtttgaa
    24421 aatgaacaac ccgtgcccca agatcactga cagacaaaga tgtcccattt tgattcgtga
    24481 tcgtaatcaa gtgatagttt tcgccaaaat tttgttcatt gatttccatg ttccttgcac
    24541 cctcttttct ttcatttgca ctgattccag tatacatgga aaccgttttc ttaacagctt
    24601 attccgtgag taacgagaaa aaatttacta tattctaata aaagaccgtg aaaaaaaggt
    24661 tgtgacaaaa atattgtcac aacctctttt tccgaacaaa cggtgttcaa aagcttgcat
    24721 ctgcggtaat aagcccaacc aaacgaaaaa tatgaggagc tatttttgct tggctgttct
    24781 tattacttga tgcaggacag ctttttcaca atctcttttt caatacgatt ccagtatata
    24841 accatgtcgt cagtacatag tagatgaaat acaatacgaa gaagatcgta aacggaaccc
    24901 aaatatcttt gtatggattt tgcatgaata gaccaaacat ttgtaatccg aataagacat
    24961 ggactacacc taagattcct ggtgctaaga ataacacgcc tacttctcta cggaccgatt
    25021 gtttaagcaa cccgcgtcgt gttcctatct tttcaagcat ttcataacga gcaatgtcac
    25081 tcttgcttcc tgacaagatc ttgaacatca aacagctagc aagcatggtc aagaaagcta
    25141 aacctaggaa gaatcccata aattcaaaac cagagaatag accattatac acttcataca
    25201 tttcatattt ctggttgctg tactctactt tttgcaaaga tggattgttc attttatttt
    25261 gagcaacaag tgtttcgatt ggtttcaggt ttgcttggaa gtcttttacc tgaactagct
    25321 gtaaagtgga ttgtggcaag ttcagttgat caaattctga ttgactgact accttgattt
    25381 ctttttcttg ctgttccggc aattcatagc ttcgaagttc ctcttgtaca gtcagttcat
    25441 tcattttttt agcaggaact ttttctttcg tgatcttcgg cggtgttcca tcttcatgtt
    25501 ttatcatgat caatggttga gccgcaaatt cacttgcatt atagtaaatc gttcccgcat
    25561 cttctttttg agtataagaa gcagaaagtg ttggtgaaag ttctttgatt ttttgctgat
    25621 cttctgcccg aggattattc atcactagat catatccaga attgttttct gtcaagtctg
    25681 caatttcatt tttaaaacct aatcctaccg tcaaagcgcc cagtgccaag gcaaacagca
    25741 tagcgaccat tgagagcatt tgtgtatatt cacgaatacg aaagtttaac tgtgacaatg
    25801 tgaaattgtt caattttttc aatgaaatct gttctgattt ctttagtaat gataaaaaga
    25861 aaatgatcac agaatggaaa atgaaataag ttcctaatac gatcgtaact aaggctaccc
    25921 ctataccaaa gacttgatag gtccccacgt gtttcatcat atagtaaccg cagcctagaa
    25981 aagcaattcc cagtactact tcaaggaaaa acaagaattt atgctgcttg attcttgttg
    26041 gtgtctgatc cgcacgaatc aatgctaaaa tcggtttttt gacaatcgaa aaagcattga
    26101 caacagcagc taataaaaac aaaacagcaa aaaacaaaac agtgatcaac aacccgtgaa
    26161 tactaaaagg tgtaaaatgc gaaatattga gatctaactg atccactaat aatcggtgga
    26221 cgatacttgt caatcctaca ccgacagctg aacccactaa agtagctgcg atccctacta
    26281 agaatgtttc agaaaaaatc atctgtgcga tttttctgcc ttttgctccc agcatcataa
    26341 acattgcata gtctttttgc cgcatcgtca tcaaaaagga atttgcatat aaaatataaa
    26401 cgaatgtgat gatccctagt aaaacagaac cgaatcggaa aatgataact gtagccgcaa
    26461 cgatcgtatt acttttcaaa aaagcttcgt tgcttgccat tgattcaaac atgtaaaaaa
    26521 tagcagatgc cataactaat cctgagaata acaccaagta gtctcttaaa cggctcttga
    26581 tcccagtaag tgataatttc caaagcatag tcaatctccc cctactgttc caagtttcca
    26641 agaatagata gtatttctcg atagaaggaa tcacggcttt gatctccacg tttcacttct
    26701 tgatggatac cgccatcctt gatgaataaa atacgttggc aataacttgc agaaaacgga
    26761 tcatgcgtca caagaagaat cgatacatga tcttttgtat tcaattcgtc catcgtatct
    26821 aaaagatccc gggcactttt ggaatccagc gcacctgtag gctcatcccc taatagaatc
    26881 gttggttgtg tgatcaatgc acgtgcggcg gcgacccgtt gtttttgtcc accggaaatc
    26941 tcagaaggat agctttccaa aatatgagag atggataaac gttctgcaat tttttggaca
    27001 cgttgcttga tttcttttgg tctgacgcct tgtaaagaaa gaggaacagc gatattttct
    27061 tgtgctgtca agctttctaa taaattgaaa tcttggaaaa taaagccaat ctcattcgca
    27121 cgaaaatctg ctagttgatt tccttttaat gtggtcacat ctttttgatt gattcggata
    27181 tgcccatctg ttggtttatc taatgtggat aaaatattca aaagtgtcga cttaccagaa
    27241 ccagaagcgc ccatgatccc aataaattcg cctttttcta catcgaagct gatgccgctt
    27301 aatgcttgcg tctttttctc attgtttttt ccgtatactt ttgttacatt ctgtacttcg
    27361 actattttgt tcatttcttt ccctaccctt taattagttt ttccagtgct gttttcggca
    27421 cgtctttttt ttcgacttct tcatacgaag tgacaccttt atttttgttc caggtgattt
    27481 ttaaatatgc tcctttacga agcggtcgag tcaattgtcc cttgaagtcc agttcttttt
    27541 ccttaccctc tttcgtatag ccaggcagga tatatctata gttgactgtt tcttgtccag
    27601 agtctgattt tgaaactgtt tttgtaccat ctgtcgtaat ttgtacatag tattcttctc
    27661 cacccataac tacgtaatct gcgattttca cccctgcaag taccacaacg atgaaaacaa
    27721 gtagaccgat cagaagtttt ttcacttttt ccccttctct cttactttat atactgatta
    27781 tagaaaaaag cgaacaattc tgccttcgaa tcaactatca ttcttctttc aattttgtca
    27841 ttttctttaa aacaactaaa aaaagagttc aagacaataa tccgtcctaa actccttctt
    27901 cctatcttac aaaaagtatg tcacttacgg tctttcatgt cgcctgtatt gataacaggt
    27961 tgcgttcctt tgtctgtaat gattgagaca agtaaattat tgatcaattg gactttccga
    28021 tcatcggtga aattgatttc ttgtccatct tcgatttgct ctaaggccat ttgagtgatt
    28081 gtgacagctc cttcaacaat ggtttgtcga gcagacaaaa tggcttttgc ttgttgacgt
    28141 tggagcatgg cactagcgat ttctgttgca taggctaaat ggttcaagcg tgtttcgatt
    28201 acttcgacac ctgctactgc taaacgctct tgcagctctt tttgcaactc gtcagacact
    28261 gccgtcgtgt ttcctcgaag cgtcaaatca ttctcattga atgtatcata cgggtattgg
    28321 ctagcgatat gccgaatggc tgtctcactt tggatttcta caaaatcttg ataatacgct
    28381 acatcgaata atgcctttgc tgtatcaacg actttaaaga ctactactgc tgagatttcg
    28441 atggggtttc catccaaatc attgactttt aaaacagaac tattgaaatt ccgaactttc
    28501 aacgaaacag tcattttttg tgccaaaggt atcgtaagga aaaagccatt ttctcgaatc
    28561 gtacctaaat attgtccaaa aaacaaaatt acttttgctt gattaggact gactacagtt
    28621 gccgaactta acaacaataa cgtgatcaac caaagaaaaa tacttaaaaa aagtgcccaa
    28681 actttcgctt gccacatacc taaatagaag aaaaacaatc caactaaagc taatacgacc
    28741 aatccgacaa tccctgcata accattgata taaaatgctc ttttttctct catgctactt
    28801 cctgcgctcc tttatccttc aataaatatc taatgttact tttttaattt ttttttcgaa
    28861 tacgcgccat tacaccttct agttctaact ctttgtcttc agtgatgtac caaccgcttg
    28921 cctgtgtccc tccggctgcc tgcggtaaat aatccaattt tgttcgaacc cagtgctttt
    28981 cttctgtaaa atagtcttct accaagacct ctagatccat ccctgctgaa agaggcaaat
    29041 cttcccgaac agatttaaag ataaatcgtt ccttcttccc atcatacttc aatattcctg
    29101 tatcgataat cggtaaagaa taataatcca gctctttctt caccgtcaat aaatgtttga
    29161 aaacttcatg gtacacttgc tctaattggc gaccttctga attgttcggg taattaacca
    29221 tcactttttc tttgtctgga tcatagaagg tttggcttag ttgagtgata taagtatgta
    29281 atctttccat ttctgttttt aacttttctc tttgaccttc ttctaccttc atatactctt
    29341 tcaccctgtt ttctttttag atattttctt tcttatttta ctatagttat gtgcgttaag
    29401 cgatgaaaaa cagcaaagat tggaaggatc tttgctgttt tttcttaaaa tatccagttg
    29461 agtacaggat caatctgttt cattgtcctc ttgatccgtc gtaatattga tcgatgtgtc
    29521 tttagcagtg atctttagct ttttctgtcc ttgttctaac aaagtatttg ttattggctg
    29581 gtagtctttt tcaatataag attcaccaga cgtttggata gtatagccac tgttttccat
    29641 ctcatgcaaa gataaattgc caccgtttag gatgactttt ccttctttgc tgatcaaact
    29701 gtcttctaga tggaagttgc tgttttcacc tgtgacgatc aagttatcca cattggcctc
    29761 atctaatcga agtgagccta attcatcttt cgcagttatt gttcctcctg taaattcatt
    29821 gaacgatacc cagccatcat gtgtcttagt attcactttt ttgattgata cttcgttgat
    29881 atcaattgca ctattattcc cttcgattgt caattcagat aattcatgat ctgcagggac
    29941 aacaatagtg atttgtgcac ttgtgtcttc taaactaatg attgcacctg ttgatttttt
    30001 gactgcattc acagtcaaca cgccattttt ctcctttatt tctggctttt ctgtcttatc
    30061 ataagtcgtt tttatctcaa aatccaatcc gcgtatgacc gaaactgttt gattttctgc
    30121 atggatgacg attttgtttg catccttgat tttttttgtc tcagtaacca tatcgatcaa
    30181 ccgaggacca ttatcccaag tcagactctt ttgagcgcct aatgtataag ttgcacctgc
    30241 taggaggcca ccagtaagca tcagaccaat accggtaatc aaaaaatgtt tcaatctcaa
    30301 tttatgtgtc ctccttcaaa cgcccgctga atgcagatct ctttttgtaa taaaacgtcg
    30361 tcccatccat ttaacaaagt tcatcatgac ataaaaaagc ccttttgttc ctttcagcaa
    30421 cagtggcgaa ataaatactg cgcttcctgt ggcgaacaga ccgacaccga taaagaataa
    30481 tccactcaga atcgattgtc caaacacact gattcctgag atgatcatga ataggccaac
    30541 acctaagata ctgatacata aaacatagat actaaaaatg agcgctagta aagcaaccaa
    30601 tagtccccca ataaccaata taaaccccag tgctagtgga atgaggacag gagaagcaaa
    30661 taaaccgata atgattaacc aaatcaggcg aatttgtctt tttggatttt tcactaactc
    30721 gttttctgct tgttcttctt ctgaaaagta actgattttt aattttaaga caaactgctt
    30781 tggtgtacca tattcatcaa tgatctgatc taatgttttt ccagaatcta aaaaatattc
    30841 ctcataatac tggatcaaat caaaacgatc gtcttcagga atttcttgaa gatgatccac
    30901 aacttcattc aaatattcat tcatttgtgc tctcctccaa taaccggtct aaagaatctc
    30961 gaaaaacaat ccattcatca ataatttctt ctaaatgacg acttcccatt ggtgtcaacg
    31021 agtaatatcg acggtttctg ccttcaaaag gttcatcata ggtctcaaga tatccttctt
    31081 ttttcaatct tctcaaaacc ggatatagtg tagattcact gacagagaga ctttcttgta
    31141 cgatttttgt taaggcatac ccatacatat cttccttgct taatgctgct agcacagtac
    31201 catcaagaag aacagttgga atctggatgt gcattacgtc accttctatt ctcaagtgta
    31261 atattttatt tcaatctata ttatacaaca tataatatag tttcaaaatc agtttgtcaa
    31321 atacaaaaaa actgactaaa agatttgatc ctttttagtc agcaaaaaat ggcttctctt
    31381 tgatttttat tataacagtg gcataatcca atttgtccac atccagctca gtggcatgat
    31441 aacgatcatt gcaggtatca tgtcagctac tgggaacatt ttcacgttga tcattctaaa
    31501 accggtagcg accatcaaga atccgccgca agctttaaaa tctaagatca tgtctggtgt
    31561 agtcaatgga taaataaatt ttgccaataa gaacaaaata taaaaaataa tgaattgggg
    31621 aattgctatc acagaaacga cataccctaa attacacgca aaaatagctg ctgtgaaaaa
    31681 atcaaggaca gatttagaaa tcaaaatcgt actgtctcct gtcatcccag aatctaaact
    31741 tccatagatc cccgtaccac ttgcacaaaa tagaacaatg accgtcacta atgtgctgat
    31801 aaattcatcg tgggtcatac caagcttttc actagggaag attttagaaa taggtacttg
    31861 catcagcgca gcccccttat ttatcccatt acctaaatga acaatcagac caatcgctgt
    31921 tcctatcact aaagcaaaaa taacagccgg catatttttc atcggcgcaa tggaatagat
    31981 tcccatcccc atcgaacaca caccaaaaac catcgtcagc tgtgctttaa aatgttcact
    32041 taatttattt cctactaatc cgccaattag tccgccaagc aaaacggaac ctacattgat
    32101 caaaactcca gttggcatca aaaaactcct ctctttttct acgaagcgaa aaaaatcaga
    32161 aaaatttctg taattattgc tttcgctgca agaaagattg taacagacag agaagattac
    32221 accaattcat tttttgtgtt aaagtattct ttattggaat aagtaggagg aatatgatgg
    32281 atatccataa attcgaagtt tttcttgatt tagccgaaac aatgaatttc accaaaactg
    32341 ctgatcgaca attcaccacc caaggaaata tttctaaaca gattctttcg ttagaaacag
    32401 aattagacgt caaattattc gaacgcgccc atagaaaaat cgaattgacc gaagctggaa
    32461 gcttattgct cccatatgta aaaaatgtcg tagaacaata ccatgctatg caagaagtgc
    32521 ttgaaaccta tacgaaagac aacaatcttt cattaaatat cttaacgata ccgacaatga
    32581 tcaattataa aggtttttcc aaaatcaccg agtttttgaa acgccatcct gaatttacag
    32641 tccagttgaa agaagtggaa agtgtcgaat taacttacaa tgacgccaat gaacatagtg
    32701 acacgatcca ctttgctaga agtttccagg ccccaccaag ttcgattgag tggttgccta
    32761 ctgaaacaga cgattttgta gcagttctac ctaaaaatca tcctttagct agtaaaaaga
    32821 aattggatct aagcgaactg aaaaaggaga actttttgct ccttggcccc actacgaact
    32881 tgtatcaacc tgtactagat ctgtgtcgcg aagctggatt tgaaccaaaa atctcttata
    32941 aaggagcaag aatcgacttg atcatcaata tgattgcaaa tgacatgggg attgctattg
    33001 ttatggaaaa aacagtcaag aatctattaa aagaaaatac tgtgattctc cctatttctc
    33061 caacaacaga gagttatctt gcatttactc gtaaagtcgg tgagcattct ttggcttcag
    33121 atacattttg ggagtattta aatcaagaag aaaactaagc cctacatttt ctgtccatct
    33181 atccacccaa aaaaagagag cgacgcatga ctttcgctct ctttcttgca tcatgtcctt
    33241 gtttcaatga cacctctaac atttctcgta gttcaataag aaagcagcga tttttatgcc
    33301 aaaagaaccg taagaacatt tccctaatca caaaaaaacg actaaaaacc cctaaaaacc
    33361 ttcaatagaa tttttagggg ttttattctt tttcctattg tttacttaac cgtatcattg
    33421 ctgtcgttcc tactccttcg atactgctat aatcaatacg tccatcatgc agttctagaa
    33481 tttttttaac cagtgaaagt cctagcccat tcccagaaat ttgacgactt gtatctcctt
    33541 gataaaattt ttcaaacatt cgatctctgg tctcttcatt cattcctaca cctgaatcgg
    33601 tcacttccag tacaatctct gtggctgtct caaatagttt gatatgaatc tgtccattga
    33661 tttgattgta ctttatggcg ttatccatga tattcagcca cacttggtac aaaaactctt
    33721 cattgcctgt gtaatttact gcagcaagtt cgatatccaa ttctagctgt tctttctccc
    33781 atttaggctg caaaaaaacg attacttctc tgatttgttc atctaatcga tactctttct
    33841 tttccagttg gatacgctgg ttttccagtt tgttcaattt taagatgttc tctgttaatt
    33901 gtgaaagctg cgtgatactt tcgataatcc gatataaaaa tatttggcgt tcttcgtcag
    33961 agagatttgg ggcttgtaac aattgaacat atccttggat tgttgctagt ggtgttttga
    34021 attcatggga tacactggac acaaagtcat tcctcagtgt ctcgatacta ttgagttctt
    34081 ggaccataac gttgaaatct ttatacagtt gctgtacttc tgctactttc tgctgttcat
    34141 ccattcgaat cgaaaaatct cccgttgcta ccagactcat atttgtccct agcgcactga
    34201 ttggatgcag gatacgccgc ccaacaaaaa tcgatattcc tgtacccacg accatactga
    34261 accccgcaaa gacgaataac gggtataacg ttggttttcc agccgattca gcaggcgaga
    34321 tagaatgtcg agacaataag aaaataaaac cgagaaaaca cagcagtgtc actaagatcg
    34381 aaatgaagac aatactagca aaatagatcc ataattgtga aaatagcttt tttttcatct
    34441 ttaaataacc gccttatagc ctagtccccg tactgtgatg atttgaaaat cttccgtctt
    34501 ttcaaaacgg gtccttaaac gtttgatatg tacatcgatc gttcgttctt ctgtattcgt
    34561 atctaatccc caaatatcat ccatcaactg ttggcgagtg aagatcttgt tgggataaga
    34621 cagtaattta tacagaagca gaaattcttt ttgaggcaga atcaacgaat gctgatcttg
    34681 taacactgta taagcatcta gctctaattt tgtttgtccg attatcaagc tactgctgta
    34741 attgatcttt gaacgtcgaa gcaatgcttc cacacgtagg atcatctcat tgacatcgat
    34801 gggttttgtc atataatcat ctgctcctaa tttgaacccg cgtttcttat cctcaaaatc
    34861 ggcctttgcc gtaatcatta gaacaggtgt gtcaaatctg gtgctacgta atgagtctag
    34921 caagtcatag ccatccattt tcggcatcat gatatctgta atgattaatt ctatacgtgt
    34981 cgcttctagt tttttcagcg catcctctcc attcaatgcg gtgatgactg aaaagcccgc
    35041 tcgttccaac acagaacggt aaagcaactg taattgcgga tcatcttcta ctactaaaat
    35101 acgattcatt gatctccctc tgttttttga aaaatttcta gattttttct tgtagttcat
    35161 cttcagttaa catttatttc gtatactacg atcattgttc tgatatactt attagtatac
    35221 cgaatatttc aatacgggag aagggtgatt atgcttcaat taaaagatat aaaaaaatat
    35281 tataaagtcg gcgaaaccac caccaaagca ttggatggtg tttctgtcgc tttcagacaa
    35341 aaagaattcg tagcgatttt aggtcctagt ggttctggta aaacgactat gctgaatgtg
    35401 attggtggac tagataatta tgattccggt gatatggtaa tcaacggaaa gtctactaaa
    35461 gactttaagg acagtgactg ggatgcctac cgaaacaact ctatcggttt catttttcaa
    35521 agttacaact tgattggtca tctaggcatt attgaaaacg ttgaacttgg gatgacatta
    35581 agtggtgttt ctaaagacga aaaacgaaaa cgggcagaag atgcattgca ccgagtcggc
    35641 ttgacagatc atatgcataa aaagccaaat cagttatctg gtggacaaat gcagcgagtg
    35701 gccatcgctc gagcactagc caatgaccca gatatccttt tgtgcgatga accaaccgga
    35761 gcgttagata cagaaacaag tatccagatc atggaattga tccaagagtt atcgaaagaa
    35821 aaattagtga tcatggtcac acataatcct gaactggccc atcaatacgc agatcggatc
    35881 attgaatttt ctgatggtaa gatcttaaca gattcccatc ctcacatcga acgaccaaaa
    35941 gatgatcaat ttaacttacg cagaacaaag atgagttttt ggacagcgtt aaagctttcc
    36001 ttcaacaata tccgaacaaa aaaaggacgt actttcttga cctcctttgc ttccagtatt
    36061 gggatcatcg gaatcgcgat cgttctgtcc ctttcttctg gattccaaaa acaaatcgat
    36121 aacacgcaag ctgaaacgat ggcgaaattt ccgattacta tttctaaggt aacgactaac
    36181 cagactcgtg acgatgccgg attgggtgca agcaaagcgg attatccaga cagcaaaacg
    36241 attactgcaa aagtcagcga agaagataaa gcacagcaca cgaataagat tgatcaaaca
    36301 tacgtcgatt acgtaacaga catcgatcct aacttaagta ataatatcgg atttacccgt
    36361 actacaggaa tcaatctttt acgtgatgta aatggcaaag tacagcctgt cagcttctcg
    36421 aatcaaaatc ctgatacgga atctctttct ttatctagta cgatgtcagc aatgacaggt
    36481 gtgggagtgt caagtttccc tacacagctt gacactagca aagaaaactt tttaaaagac
    36541 aattattctt tacttgccgg aagttatcca gcctcagcaa cagatgtcgt gctgatcgta
    36601 gacggcaata ataatactaa tatcaacgca ttgaagaatc ttggatttga tgtgaaagaa
    36661 gacgaaaaac ttgattttga tgatatcgtt ggtacaacat tcaaattagt aaacaacaat
    36721 acttactata caaaacttcc aactgggaat tttatcccta atacagatta tgatgcgatg
    36781 tatcagaatg cttctgatga attgaagatc tcggggatcc tacgcgtgaa atcttcttct
    36841 acaatgaatc tcttatctcc aggaatcgct tatagcgatc aattaacgac tcaaatcgtc
    36901 aacgaaaaca aagaatcaga aatcgtcaaa gctcaaagag acagtggtgt aaatgtctta
    36961 actactgaaa aagttgacga aaatgccaaa caaacattgc ttagttattt aggtggagac
    37021 agcttgcctt caagtatcat gatttatccg aacaattttg aagacaaaga aaagatcttg
    37081 gattatctag atgattacaa taaaggcaaa tcagatgaag ataaaatcat ttatactgat
    37141 ttagctggaa cgatgactga actgactggc ggattgatgg atgctatcac ttatgtcctt
    37201 attgcttttg cagggatctc tttagtaaca agtatgatca tgatcagtat cattacttat
    37261 acatctgtga ttgaacggac aaaagaaatc ggtgtactaa aagctttagg tgcacgtaaa
    37321 aaagacatca ctcgcgtatt tgacgcagaa acgtgtattt taggtatttc ttctgggata
    37381 ttaggtgtgt ttattgcatg gctagctacg ttccctatca atagcatctt atacaacatg
    37441 acggatctaa aaaatgttgc tcaactaaat cctgtccatg cgattattct agtgatcgtc
    37501 tctactatat tgacgatgct tggcggtcat cttcctgcac gaatggcagc gaaaaaagac
    37561 gccgctatcg ctttgcgtgc agaataaggt tgtgacaacc acatttagat ttgagtcata
    37621 agagcgctga aatagtagaa aacagcaaga aacgaggaag taatatgttc cttgtttcgc
    37681 gctgtttttt ttaattattt tttataattc tggtatactt ttctttcctt tgtaccacaa
    37741 ctcttctggc gtatacctgt cttttccatt tatgaatact gttccatctg cttcaatcgt
    37801 tacttcagtc ccaatccaca tattacgaga aagtgcgaga atattttttc tttttcctct
    37861 tccttttttt ccaaattgca aggcatcggt tatctcttct gtccaatgag atttcacgta
    37921 ttttacttct ccgttttcaa atgtaacttt caaaatattc gctatatttt ccacttttac
    37981 tgctttagga aatgactcct tcattttttt cactttcctt cctccaacac taagtatgcg
    38041 ctttctttta gtgataagta tatcatagtt tattctatta tcaaaaaaac gcatccattc
    38101 cattgtagaa tactttaccg aacaagttga attgttttcc tctttttcac cttttactat
    38161 agataggtaa agacaactaa ggaggaatct ctcatgacag atcaaccgta tgatttaaga
    38221 aaagtcatcg aagaactaaa aacacttcca ggtcaatatc acgaaacaga tattgaaatc
    38281 gatcctgaag cagaattatc tggtgtttac cgttatatcg gtgctggcgg tacagtaaaa
    38341 cgtccgactc aagaaggacc agctatgact ttcaacaata tcaaaggttt ccccggcaca
    38401 cgtgtgaaca tcggaacaat ggctagtcgt aaacgtgtag gtgaaatttt gcactatgat
    38461 tatcgaacac ttggtcgttt cttgaaagac gctgtagaaa atccaatcaa acctgtgaaa
    38521 gtagacaaag cagacgctcc agcacaagaa gtcgttcatc tagctaccga tgcagacttc
    38581 gatattcgca acattttgcc tgcacctaca aatactcctg aagatgctgg tccatacatc
    38641 acaatgggtg tagtcttagg atcagatcca gaaaaaacaa tgagcgatgt aacgatccat
    38701 cgtatggttc tagaagataa agatacgatc ggtatgtaca tcatgcctgg cggacgccac
    38761 attggtcact tccaaaaaca attcgaagcc ttgaataaac caatgcctat cactatcaat
    38821 attggtttag atccagcaat tgccattggc acgactttcg aaccacctac cactccactt
    38881 ggctacaatg aactatggtg tgcaggagct ttgcgtaacg aacctattca aatcgttgat
    38941 ggtatagcag tcgatgaagt gggtattgct cgtgctgaat tcatcattga agcagaaatc
    39001 atgccaaatg aaacaatgca agaagatatc aacacgaact caggaaaagc aatgccagaa
    39061 tttcctggct atactgggga tgctaatcca gctgtacaag tagtgaaagt caaagcagtt
    39121 actcaccgaa aagacaatcc aatcatgcaa acaacgattg gtcccagtga agaacacgta
    39181 tctatggccg gtattcctac agaagcaagt attcttgcct tggtcgataa agctattcca
    39241 ggaaaagtag taaatgtcta caatcctcct gcaggtggtg gtaaattgat gaccatcatg
    39301 caaatccgta aagaaaacga agcggatgaa ggtattcagc gtcaagcagc tattcttgct
    39361 ttatcagcct tcaaagaatt gaagacagtc attttagtcg atgaagatgt tgatattttc
    39421 gacatgaacg atgtagtttg gactttcaac actcgtttcc aaggagataa agatatcatc
    39481 gtgatcccag gaatgaggaa ccatccattg gacccatcag aattaccaga atataatcct
    39541 ggctctattc gtttccgtgg gatgagtgca aaaactatct ttgacggtac agttcctttc
    39601 gatatgaaag aacattttgt acgtgcaaaa ttccaagaag tagaagattg ggaaaaatat
    39661 ttgaaataat gaatgatctg caactgtttt gacatcataa aaaggagtgc ggaaaatgga
    39721 acaattggca caacggaaga aaaaaatcgt gattggtgta agtggcgctt caggcaccat
    39781 ttatgccatc aacctagtga agaaactaaa agaatatccg atgatagaaa cacacggaat
    39841 catcagtcca tgggcaaaac agaacttaaa gctggaatcc gacttatcac taactgaatt
    39901 cgaaagcctg tttgattacc attattcaaa caaagattta ggtgctgctg tcgccagcgg
    39961 atcatttttg accgatggaa tgatcatcgt accggcaagt atgaaaacag tcgcaggtat
    40021 ctctgttggt ttaggcgaca acctgattgc acgtgctgct gatgtaacaa tgaaggagca
    40081 accaaagtta gtcattgttc ctcgtgaaac gccattaaat gccattcact tggaaaactt
    40141 gactaaatta gcacgactag gtgtgcaaat cataccacca attcctgctt tttataatca
    40201 gcctcaaacg atccaagatc ttgttaatca ccatacaatg aaacttttgg atcaatttgg
    40261 aattgtaaat gagcattcaa aacgttggca gggtgattaa tatggaaaaa gaaacgatag
    40321 ttgaagtaac caaactaggt ttcacgatcc aagctgctgt taaagtaatc ggtaaagact
    40381 tattgatcac tttaacaggc ggagatacac cacatatcgg aacagtcact acattttctc
    40441 gtgatacaga gatgcaaact gtacgtttcc ctagccacga tggtcggttc cataaagacg
    40501 atgtcttagc cgaaaaaata gctgtgatca tcaaaactgc cgttccggga agttgtacga
    40561 tcacttccgg tatccatgtt gaccatatct ccaatgcaca aatcaacgcc tcttttccga
    40621 tgtcagaaga gttaggttac aagattttag attggttgga aaaaatagat ttccatatca
    40681 aagaaccagt ttattataaa gatggtgaaa aaccattatg aaagaagagg ttgtgaaatc
    40741 agcgttatga cctgagtatg agagaaaaaa atggaaaaat agcttctcat attttgccgt
    40801 ttttgagcta ataccgaggg acagctgatt gaacgccgtt tatcaagagg ttgtgataaa
    40861 gccgcttcgt ttcgagtagt aagaaaaatt ttgtaaacat gggtttccac atttttgcat
    40921 aaatttgact tactaccgag aaactggctt ttgaacaccg tttatcaaga ggttgtgaca
    40981 cgtcttttgt cacaacctct tttatttttg cttcactgta actgctacaa tatcaccgat
    41041 ttcttttccg atgtttttac gaatatcttt tcgtagtcca aggatgtagc agactgaacc
    41101 atctggattt ttcacgccca tattcacgat actgccttca tacggcacat tgtcaaaagt
    41161 tgcttggact ttaactcttc cagcaccgaa ttcttttctt atatcatacg gaaaaataat
    41221 atatgccccg cccttgtcga cttcggaagc atagattttt gattcaaaag aataaatttc
    41281 cctcatttta tttctcctat tttttacatc tagtatagca tagcaaaaaa aagaaggaac
    41341 tgtgacaaaa gtcttgtcgc agttcctttt ttcgaataaa cggtgttctc ctgccaactc
    41401 ctcggtcaca agtcacaata gtcaacaaac atgagaagct gttttttgaa attgctcctt
    41461 gtgactcaga gttaaacggc aggctcacaa cctcttcct
//
LOCUS       DAFFSV010000011        40192 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFFSV010000011 DAFFSV010000000
VERSION     DAFFSV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.011"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            252..743
                     /gene="nrdR"
                     /locus_tag="LR110_000506"
     CDS             252..743
                     /gene="nrdR"
                     /locus_tag="LR110_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBL4821510.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            766..2139
                     /locus_tag="LR110_000507"
     CDS             766..2139
                     /locus_tag="LR110_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL4821511.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            2139..3065
                     /gene="dnaI"
                     /locus_tag="LR110_000508"
     CDS             2139..3065
                     /gene="dnaI"
                     /locus_tag="LR110_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBL4821512.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            3194..3634
                     /locus_tag="LR110_000509"
     CDS             3194..3634
                     /locus_tag="LR110_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL4821513.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            3778..4479
                     /locus_tag="LR110_000510"
     CDS             3778..4479
                     /locus_tag="LR110_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBL4821514.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            4476..5597
                     /locus_tag="LR110_000511"
     CDS             4476..5597
                     /locus_tag="LR110_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBL4821515.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            5612..6844
                     /gene="pepT"
                     /locus_tag="LR110_000512"
     CDS             5612..6844
                     /gene="pepT"
                     /locus_tag="LR110_000512"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBL4821516.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            6976..7884
                     /locus_tag="LR110_000513"
     CDS             6976..7884
                     /locus_tag="LR110_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBL4821517.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            7919..8191
                     /locus_tag="LR110_000514"
     CDS             7919..8191
                     /locus_tag="LR110_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBL4821518.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVNLILIYFFH"
     gene            8202..9248
                     /locus_tag="LR110_000515"
     CDS             8202..9248
                     /locus_tag="LR110_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBL4821519.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            9499..12108
                     /gene="clpB"
                     /locus_tag="LR110_000516"
     CDS             9499..12108
                     /gene="clpB"
                     /locus_tag="LR110_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBL4821520.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(12259..12945)
                     /locus_tag="LR110_000517"
     CDS             complement(12259..12945)
                     /locus_tag="LR110_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821521.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(12923..13417)
                     /locus_tag="LR110_000518"
     CDS             complement(12923..13417)
                     /locus_tag="LR110_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL4821522.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(13437..14657)
                     /locus_tag="LR110_000519"
     CDS             complement(13437..14657)
                     /locus_tag="LR110_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4821523.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            14804..16639
                     /gene="lepA"
                     /locus_tag="LR110_000520"
     CDS             14804..16639
                     /gene="lepA"
                     /locus_tag="LR110_000520"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBL4821524.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            16835..17746
                     /locus_tag="LR110_000521"
     CDS             16835..17746
                     /locus_tag="LR110_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4821525.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            18742..18993
                     /locus_tag="LR110_000522"
     CDS             18742..18993
                     /locus_tag="LR110_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821526.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            19076..19348
                     /locus_tag="LR110_000523"
     CDS             19076..19348
                     /locus_tag="LR110_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821527.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDRYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            19438..19677
                     /locus_tag="LR110_000524"
     CDS             19438..19677
                     /locus_tag="LR110_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821528.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            19824..24107
                     /locus_tag="LR110_000525"
     CDS             19824..24107
                     /locus_tag="LR110_000525"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBL4821529.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            24206..24571
                     /locus_tag="LR110_000526"
     CDS             24206..24571
                     /locus_tag="LR110_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821530.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            24573..25025
                     /locus_tag="LR110_000527"
     CDS             24573..25025
                     /locus_tag="LR110_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821531.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            25639..26949
                     /locus_tag="LR110_000528"
     CDS             25639..26949
                     /locus_tag="LR110_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBL4821532.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            26972..27283
                     /locus_tag="LR110_000529"
     CDS             26972..27283
                     /locus_tag="LR110_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821533.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            27644..28900
                     /locus_tag="LR110_000530"
     CDS             27644..28900
                     /locus_tag="LR110_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL4821534.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            28897..29442
                     /locus_tag="LR110_000531"
     CDS             28897..29442
                     /locus_tag="LR110_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821535.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            29443..29943
                     /locus_tag="LR110_000532"
     CDS             29443..29943
                     /locus_tag="LR110_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821536.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            30024..30580
                     /locus_tag="LR110_000533"
                     /pseudo
     CDS             30024..30580
                     /locus_tag="LR110_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30600..30944
                     /locus_tag="LR110_000534"
     CDS             30600..30944
                     /locus_tag="LR110_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821537.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            30963..31874
                     /locus_tag="LR110_000535"
     CDS             30963..31874
                     /locus_tag="LR110_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL4821538.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            31953..32945
                     /locus_tag="LR110_000536"
     CDS             31953..32945
                     /locus_tag="LR110_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL4821539.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            32942..33160
                     /locus_tag="LR110_000537"
     CDS             32942..33160
                     /locus_tag="LR110_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821540.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            33173..33583
                     /locus_tag="LR110_000538"
     CDS             33173..33583
                     /locus_tag="LR110_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821541.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            33635..36163
                     /locus_tag="LR110_000539"
     CDS             33635..36163
                     /locus_tag="LR110_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL4821542.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTVLEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            36179..38389
                     /locus_tag="LR110_000540"
     CDS             36179..38389
                     /locus_tag="LR110_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821543.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            38397..39488
                     /locus_tag="LR110_000541"
     CDS             38397..39488
                     /locus_tag="LR110_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL4821544.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            39509..40051
                     /locus_tag="LR110_000542"
     CDS             39509..40051
                     /locus_tag="LR110_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821545.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
BASE COUNT        13680 a         6445 c         8583 g        11484 t
ORIGIN      
        1 tttttgtgct agatgactaa atcagctttt gtgtaagtta tttctcagta atcaaggtga
       61 tcagtattgg aaaattgaaa tttagtaagg acaggggaca caattacgca tgaaagtaga
      121 aaaaattgtt cttatatccg tgttttttgg gtaggacagt cgcgtttgtg atttgtttcg
      181 tgttataata caactagttg taaagaaaga cacatccttt atataaaatg cgatgcaaga
      241 ggtgagacaa aatgagatgt cccagatgtc aacataataa ttcacgagta atcgacagcc
      301 gtcaagcaga tgatggacgt gcgatccgtc gccgtcgcga atgtgagaat tgttcgttcc
      361 gattcactac atttgaacga attgaagctg ctccgttatt agtaatcaag aaaaacgggg
      421 aacgcgaaga gttcaatcgc gataaaattt taagaggact catccgttca gcagaaaaaa
      481 gacctgtagc tatggaacaa atggaacaaa tcgtcaacca tgtcgaaaac cgcgtacgca
      541 gtctcggaga aaacgaagta tcgacgaatc taattggtga atatgtgatg gaagatttag
      601 ttgatttaga cgaaatcgct tatattcgtt ttgcaagtgt ttatcgccag ttcaaagata
      661 tgtctgtttt cttaaaagag ttgcaagata tcgtggataa agcaaaagaa aataataaag
      721 agaataataa agaaaacaat taagaaacaa aaaggaggca tttccttgga aaatgcttgg
      781 aaagagcttc aaccaaaaaa tatttaccaa ggaagaaaag cggctccgct gagagaagac
      841 agcaagaacg ccttgctgta tctttatcag ccactaatcg gcggagaagc cttgagtatc
      901 tatttcaccc tgttatcaga aatctctcaa gaatctggca aaggtcctga agggatgcat
      961 gcagatctgc ttagtagtct aggctgcggt atcccgcaat tttatgatgc aagaaaaaag
     1021 ctagaaggaa tcggcttact ggatgtttat tttaaagaag atagtgatct gggacctcgt
     1081 tttatttatg agctgttaga accactcatt cctaaagcat ttttcaaaga ctctgttctg
     1141 gcttttcttc ttctggaaaa agttggtgaa cgacgattcc agaagcttac ccaattcttt
     1201 gaacctaaaa ggcatacagc agagggatat caaaaaatca caaaaaaatt tttagatgtt
     1261 tatcgcttca atgaagcagt gtacgctgct aatcaagaaa acatcgaatc gctgaataaa
     1321 tcttttgaag aatctgaatc agtagcatta cttgaagcag attcgacatt cgattggcag
     1381 tttttgaatg attggctggc aaaaaaacat attgcaagtc ttgatgatcc aatcatcaaa
     1441 caagtcgaca tgtatcatca gctttacggt ttcgatgaat tgacacttgg cgaactgatc
     1501 agtcaatctt atgactttag tgaacaaaaa gtatctgaaa aagaactgca aaaagccatt
     1561 ttggcaatgc agagacgtcc ggtgaagcaa acaaaagaaa cagttgcggt ttctgaaaac
     1621 agtaatgaac cctcgtcaaa cgaacatttg acaaacgcag cgcttcagct gataaaagaa
     1681 gcacagagca ttccaccgat gaaatatctg gaagcaatca agtcagaaaa aggcggatat
     1741 gtttctaaac aagaaaactg gttactgcaa gatcttgttt ctcaatcagg tctttccagt
     1801 agcgtgatca atgttttgct gaattatgta ctagtgatca agaataatgc tagcttaaat
     1861 gccagttatg taaatacagt ggcaaatgaa tgggcacaaa agaaaatcgc cacagcagaa
     1921 gaagcgatcc agcacattcg gcaaataagt aatcaagcaa aacaatcaaa acaaaagaag
     1981 caaacaaatt atcaaactgg caagcgaaat gttcgacggg aaaagcttcc tgattgggtc
     2041 aaccagccaa aagatgaaaa acagatcagc agcgagaaaa aagcagagat tgaccgccgt
     2101 tttaaagaat acctggcaaa aaaggagggt gatatctagt ggaagacgta ggcaaagaat
     2161 tgaagaggat catcagcaaa cgcaattatc aagaaaagta tgctgaaatg atcgctgaag
     2221 tcttaaatga tgcggatgtt caagcttttc taagcgcaca ccaagaagaa ttgacacaag
     2281 cggatattga aaaaagttac gctaaattgt atgaattcgt gcaagaaaag cgtaaatatc
     2341 aaaaaaacga tccaacgatg atcgctcccg gctatgagcc gaagctgacg ttgaattttc
     2401 attatgtgga tgtcacttat gtaccaacgg aagcattgtt agcacggcaa agacaagaag
     2461 aaatcagaag ccgagtaaaa gcaatggata tgccaaaaga tattcaatcg gcttcttttg
     2521 aaaactttga acgtacagat ggacgaggat atgcgagctt agaagcccta gattttgtag
     2581 agaaatatac tgctgatcca aaagggtatc ataaaggact gtatctagta ggaagttttg
     2641 gtattggaaa aacatattta ttaggagcaa cagcgagaga attagcgatt gctggattca
     2701 ccacgacgct agtccatttt ccgacatttg ccgttgagat gaaacaggca atcggaaaag
     2761 atcaagtggc agaaaagtta gatgcagtga aacgttcacc tattttgatg atcgatgaca
     2821 ttggtgcaga tgcgatgagt agttggatac gagacgaagt attcggtgtt atccttcaat
     2881 atcggatgca ggaacagtta ccaaccttct tctcatcgaa ttttacaatg gatgaattgg
     2941 aacagcattt gtccgtcact caacgtggag atgaagagcc gctaaaagcg aaacggataa
     3001 tggaacgcat acgttatttg acaaaagaaa tcgaaatgac tggtcgcaac cgacgcaatg
     3061 gttgaacagt tgacaagtcc aactgacatt tgacctagta gtaaaagcag gtcagcgaat
     3121 cattatatgg acagaaatga ccggagacaa tcaccgcaac aagtaatgca gaaacttaaa
     3181 cggaggattt tttatgaggg ctattttttc tagtatatct gtagttggtc tattttttct
     3241 tttagcaggc tgcggtagcg cagatagcaa cacaggttct gttgattcga ttgatgcggg
     3301 tcaaacagta aaaacaagca gtacgaaaac ggagagacaa agcatatttg tcggaacgat
     3361 tgaaagtatt actcaagtag aagcagaaga agaaacgctt cagttgaaac tcgtcgatgt
     3421 catggccaaa gaagatccag cgaatatggg aacgtcattc agcaatgacg gagtgatctt
     3481 aaatgctact gctgaaaaaa taagcgtgga cataaatgaa ttgaaaatcg gtgacaaagt
     3541 ccaatttaca ttagttgaga tgccgatcat gacgatgtct atcccaccgc aagttcctgg
     3601 agattcgatt attgatgttt cattgaacaa ataaccaata acattttttc aaaaataaga
     3661 aaatgttgtt ttatagaacg aaaaggtata ttttttaagg agatatggga atttttttat
     3721 tcctagtctc cttattttat ttggaaaatg ctataattat ctagaaggag tgaagctatg
     3781 aaccatttag atttatctca gcgcttagcc gcagtgggtg actttgtccc tgaatccgct
     3841 cgtttagcag acattggatc agatcacgct tacttacccg ttgcattgat attaagagga
     3901 aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc agcacgtaag
     3961 caagtcaaaa aagatggatt agaagaccgt atcaccgtac gccttgcaaa tggtctagat
     4021 gctatccagc cacaagatgg tattacagct gtcacgattg cgggtatggg aggttcactg
     4081 atccgagata tcctagaagc tggcaagttg aatcaacgct taagcggaca agaacgtttg
     4141 atccttcagc caaacatcgg agagcgtaca ttacgatctt ggctgcaaga aaatcagtat
     4201 aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat cattgtagcc
     4261 gaaaaagaac aaaaaccagt tacttacaat gaaatggagc tgatgttcgg gccatttctg
     4321 cttgaagaga aaagtgagat cttcaaatca aaatggacaa gagagcttca acaaagaaaa
     4381 gctatccgtg aacagctcaa acaagcagct aatcaagagg cacgtcaaaa acaaatcgag
     4441 caggaaatcc aaatgattga ggaggtttta aagccatgac aatcagcgga cgcactttca
     4501 tccaaaagtt tgaaagctat tgtcctcagt ggctagctga agaaggagac ccagtcggac
     4561 tgcatatcgg aacattggat aagccaatcc aacgagtaat gatgacatta gacgttcgtc
     4621 cagaagtggt cgaagaagca atcaagaaaa aaatcgattt gctgattgct aaacatcctc
     4681 caatctttcg tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa atgtatgcag
     4741 atttgttgaa acatgatatt gccgtctatg cagcacacac gaacatggat attatttggg
     4801 atggactgaa tgactggttt tgtgaacttc tcggtattga agtggagagt tatttagtaa
     4861 agactcatga gattcattat aaaaaactgg ccgtttatgt acctgtggat catgcacaga
     4921 aaatgcgtga agtattagct gcagctggtg ctggtacaca aggtgattat accggtacaa
     4981 gttttacctc gattggacac ggtcgattta ctccggaagc tggtgcacaa ccagcgattg
     5041 ggaaagtagg gaagacagaa caagttcaag aggcaaaagt tgaggttatt ttgccggaaa
     5101 ctatcgaaaa acaagtgatt caagccatgc ggtcagctca tccttatgag gaacctgctt
     5161 atgatctttt cgctatagat gaaccagtcg aaatgtttgg gttaggacga gtaggtgaat
     5221 tgccgcaaga aatttctata gaggcatttg tagaacaagt aaaagaagcg ttccaactag
     5281 atggactgag aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc attgcgattt
     5341 gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca gatgtctata
     5401 tcacgggaga tatctattat catacggctc atgatatgca aagcgctggg ttgattgcaa
     5461 ttgatcccgg tcattatatt gaatcgttat gcaagcaacg atttatcgag aaatttgaat
     5521 cttggaaaca agaagaaaat tgggatatta actttttcgt ttctgaaaca aatacaaacc
     5581 catttcaatt taattaaaag gagagaatac tatgtacgaa aatcttttgc cacgtttttt
     5641 acgatatgtc aaaacagaaa cacgctccga tgcaacaagc aaaacgactc catcgactca
     5701 gacacaagtt gcttttgcgc aagaattgaa aaaagaatta gaagagttag gcttagctga
     5761 tgttttctat aatgaagata acggattcgt tatgggaaca ttaccaagca atgttgatca
     5821 tgaagtgcgt tccattggat ttatttccca catggataca gcagatttta atgcagtagg
     5881 agtcaatcca caaatcattg aaaactatga tggcgagtct acgatcgtac tagatgcgga
     5941 agaacgctat acattgaata caaaagattt tccaaatttg aaaaattatg aaggccacac
     6001 attgatcaca acagatggca gtacattact gggagcagat gacaaatcag ggattgcgga
     6061 aatcatgact gcaatggaga tcttgatcaa taatccttct atccctcacg gtaccatcaa
     6121 agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg atgttgagca
     6181 attcgatgta gattttgctt atacagtcga tggtggtcct ttaggtgagt tgcagtatga
     6241 aacgtttagt gctgctcaag caaatattac gatccaaggg aaaaatgtac atccagggac
     6301 ggcaaaagat acaatgatca atgctctaca gttagcaatc gattttcata atcagcttcc
     6361 agccgatgaa gttccagaaa aaacggaagg atacgaaggt ttcttccatt tgatggcatt
     6421 ggatgggaat cctgaagaag cttctatgag ttatatcatt cgcgatcatg atcgtgaaaa
     6481 attcgaagca cgcaaagcac aaatcacaca tatccaagaa gagttgaatc agcgatttga
     6541 tcaagaacga atcaaagtag atatgtatga tcaatattac aacatgaaag aaatcattga
     6601 aaaagatatg agcatcgtgg aattagcgaa acaagcaatg atcgagcttg gtatctctcc
     6661 agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa atctcttaca tgggtattcc
     6721 gacgccaaat ctatttgccg gaggagaaaa tatgcacgga cgattcgaat ttgtttcttt
     6781 acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga acacaaagag
     6841 ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg aatgtcatct
     6901 gtctaaggct aaagggcagg acaatggaat tggttgtccc gccctctttt ttgaagagta
     6961 aataggaggt aaagaatgga aaagctgcta gttttttata acaaaagttc agggaaagat
     7021 gagggtgaga aattagcacg ctggtttcaa gattatgtaa aagaaaagca gccagaatta
     7081 tatgtagaac tagctgaaac tggcccatcg attgatgatg cggaattagt caagaaagca
     7141 gaagccatag aagctgatac tttagtaatt ataggtggag acggcacgat tcatcatatc
     7201 gtccaagcgt tccaaaaaac attatctcaa taccatgtcg gattacttcc aggtggaact
     7261 gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc tgctgacgtg
     7321 atcctggaag gcaatgtacg gaaaatagac tatggtcaag tcaatgatga agtcattatc
     7381 tctacactga caatcggaat cttagcagat acagcagttt ggatcagtca agaagaaaaa
     7441 cagcgttacg gctcgtggat ctttatcaag aagtttttta agttattgct gaagaagaaa
     7501 aaatatcatt tggatttaga aacagacgag gagcattgga aaggaaaatc ccaactgttg
     7561 acagtcacga tgtctaactc cgtgggcggt tttacaaatt ttgacgactc agcgacccta
     7621 gatgatggaa aatttcatgt aacgatcgta ccttccttag atattttccg atttattttt
     7681 tatttgccta gaatcatgag aggcaaattc tatacgatcc ccggcattac ttatttcaca
     7741 gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac ggatggagac
     7801 acgacagatg atcttccagt tgaattatct gttgtatcaa aaggattaac agtttattca
     7861 ccaaagaaag aagaaaaagc ttagaaatag tagttaacag aaagttggaa gaagagtaat
     7921 ggatgtattg ttacacaatc ttttgtggtt ttaccttttc attgtgaatt tttacttatt
     7981 ttgtctgatg gggtatgata aacatcaggc tagaactagc ggctggcgtg ttcctgagaa
     8041 taatttgata tttgtcagcc tgttaggcgg tggtttggga ggattgatct ctcgaaaagt
     8101 tttccatcat aaaactcgga aaaagaagtt tactatagga ttcgctgcag gtgtacttgt
     8161 taatttgatt ctcatttatt ttttccatta aggagcagga gatgtcgtct aaaggaacga
     8221 aaactaaaat cattctgaat ctgcttttgc tgattgtgat ttttgccatt atttattatt
     8281 taatcaatca atcatttgca gatatctttg ccgaattgct gtctaccagt gttgttgttt
     8341 tggcagcggc tgttatatta gggacagttt accaaatcgc tgaagggcgc tcaatcaaag
     8401 aaattgctga acctttcgcg ccgaatttta caacgaaaga tgggttcttg acctctagct
     8461 atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg gagatttatt
     8521 tttataacaa aaaaggaatg aagctatccc agggagcagg ggtgacagca ctccatatga
     8581 ttatgtacaa actggcagtg atcacttatg cattagctgg cttggtcatc caattctctc
     8641 ttttttattc taatggtcca tcaatgatct ggtttatcat cgctggtatc cttttgacga
     8701 tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa atattttttg
     8761 tcagcttatc gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg gatacatgca
     8821 atcagcagat ctactcttta agggaaacgg tccaaaccat tatccaagac aggactgcgc
     8881 tattgcggat ttatctatgg aatatgttaa aacttgtatt ttggtatgta cttccttatt
     8941 tgattctgat tgaaaaccat cctaaaatag attttctact gaccatttcg tttatcagct
     9001 ttgcagtagt gctttcaggg gttattccta cgcctgctgg tattggttcc tttgaatttg
     9061 tctatttgtt attattcaga ccagttgttg gaacagttga cgcagtttct tcgcttttac
     9121 tgtatcgttt cagttccttt atcttgccat ttctctatgg atttgtgtat gtgattgctg
     9181 aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat aaagaaaatc
     9241 aagggtaggt cttttataag atctaccctt tttatcaaat caaagaaaaa cgttatggat
     9301 aagataattt tacgtttgtt tggctctttt tttatataca tacaatcaat tttgtttttt
     9361 taagatttat ataagtcgag aaaaaaagtc aaaaaaccat gtgtatattt tgaccaaaat
     9421 tgaccaaagc gatataatag tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa
     9481 ttaggagggt atacctgtat gaatattgag aaaatgacta cgacgcttca agaagcgatt
     9541 gctgaagctc aacagattgc ggtcactcga aaacatcaag atatcgacat tgcccatgta
     9601 tggaaaatct ttttacaacc taatcatttt ggtcgaaatt tctatacgga tgccggattg
     9661 gatgtcgaat cttttgaaca tgaaattgat cgtctactgg atgagtatcc agttgtttct
     9721 ggtggaaacg tccaatatgg acaaaacctc agccagaatt tctttcgatt attgaatgaa
     9781 gcagaccaaa tacgtgaaag cttcggcgac gagtttcttt caacggaagt cgttatttta
     9841 gcgttgatga aattaaaaaa ttatccgtta actgtttatt tgaataaaaa cggattatct
     9901 gaaaaagaat tgcgtaaaaa catcgaagag atgagaggag gagaaagagt gacctcacaa
     9961 aaccaagaag aacaatacaa agcattagaa aaatacggcg ttgatttagt acagcaagtc
    10021 aaaagcggaa aaatggatcc aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt
    10081 attctttcac gaaaaacaaa aaataatcct gttctgatag gagaacccgg tgtagggaaa
    10141 acggcaatcg ttgaagggct tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta
    10201 aaagataaaa cgattttttc attagatatg ggtgcattga tcgctggagc aaaattccgt
    10261 ggtgaatttg aagaaagact aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt
    10321 atcctcttgt tcatcgatga aatccacaat atcgtagggg caggaaaaac agaaggaagt
    10381 atggatgccg gaaatctgtt gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt
    10441 gccacaactt tagatgagta ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc
    10501 tttcaaaagg tattagtcaa agaaccaaca gtggaagata cgatcagtat tctgcgtgga
    10561 ttaaaagaac gttttgagat ccaccatggt gttaacatcc atgacaacgc attagtagca
    10621 gcagctactt tatctgaccg ttatattact gatcggttct taccggacaa agcaatcgat
    10681 ttgatcgatg aagccagtgc aacgattcgg gtagaaatga actctatgcc aacggaatta
    10741 gatcaagtta ctagacgttt gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa
    10801 tcagatgatg caagtaaaaa acgcttgaaa aatttgcaag aagaattagc tgaacttcgt
    10861 gaagaagcaa atgcgatgaa gatgcagtgg gaaacagaaa aagaagaagt caattctgtt
    10921 tcagcaaaac gtgctgaaat cgataaagca aaacacgaat tagaagatgc agaaaataac
    10981 tatgatttag aacgcgctgc tgtattgcgt catggtacga tacctcaatt agaaaaagaa
    11041 ttgaaggaat tagaagctaa agcaaaagac agcgagatta agatggttca agagtccgtt
    11101 accgaaaatg agattgctca agtcgttggc agattaacgg gtatccccgt aacgaaactt
    11161 gtagaaggtg aacgagaaaa actaattaaa ttgaatgaaa cacttcataa acgagtgatt
    11221 ggtcaagatg aagcagttga tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg
    11281 caagacccaa atcgtcctct aggttctttc cttttcctag gaccaactgg tgtcggaaaa
    11341 accgaattag cgaaagcgtt agctgagaac ttgtttgact cagaagatca tatggtacgg
    11401 atcgatatga gtgaatatat ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca
    11461 ggctatgtag gttacgaaga aggtggtcaa ttaacggaag ctgtcagacg aaatccttat
    11521 acaatcgtct tgttggatga aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta
    11581 caagtcctag atgacggacg cttgactgat tcaaaagggc gagtagtaga tttcaaaaat
    11641 acggttctga tcatgaccag caatatcggt tcacagttac tgctggaagg cgtgactgct
    11701 gatggaacga ttccagaagc agtggctgaa caagtcaaca cgctattacg tggaaacttc
    11761 aaaccagaat tcttgaaccg aatcgacgac acgattttat ttacaccatt gagcctagac
    11821 aacgtgaaag gaatcgttga caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa
    11881 gaaatcctgc tgacaatcag tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa
    11941 ccagcatatg gtgcaagacc attgaaacga tttatcacta aggaagtaga aacaccacta
    12001 gctaaagaaa tcgttgcagg acatgttatg cctaaatcaa aagtaacgat tactttactt
    12061 gatggacaac ttcatttcaa aacagaagag ctagaagaaa tcgtctaatc ttccattgag
    12121 actataaaag aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa
    12181 cagtgttcca gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt
    12241 ctgtcacaac ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta
    12301 acgctcatct tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt
    12361 atttgtcagc aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc
    12421 atcagaagca gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca
    12481 gaactcccaa aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca
    12541 aggactggtt tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag
    12601 agctgtcagg accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact
    12661 gatcaaatgc atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa
    12721 aacgattgct gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat
    12781 caatgcaatc cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt
    12841 ttgtgtgata aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc
    12901 tatttgcacc tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta
    12961 cttctgaaag caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta
    13021 ttgtatataa gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt
    13081 ttttcgggtg ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc
    13141 ctttcgcata agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta
    13201 gtaaagcaat cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc
    13261 gactgtctgc ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg
    13321 aaagctcaga agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac
    13381 ttttagcaaa cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa
    13441 aaataatcgc taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt
    13501 tcttcatctg tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc
    13561 atcaagcaag tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag
    13621 acacggactt gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg
    13681 acaggatctt caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga
    13741 aggaattctt ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc
    13801 tgactatctt caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag
    13861 tagatcatgg aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca
    13921 agatagaaca tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct
    13981 cttggttcat ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa
    14041 cgccaatact cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca
    14101 aatttgtcat aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca
    14161 cttactggga tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac
    14221 atgatttccc atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc
    14281 cattgcttat tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca
    14341 tggttagcat attctggata agttccaact ccagtcacac cacccatttt ataacgtgca
    14401 ccgtgaatat tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg
    14461 aaccatccga acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt
    14521 ttattctcaa acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc
    14581 aattcattga attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta
    14641 aaatcttttt gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat
    14701 tctacaaaaa aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt
    14761 tggtataatg gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat
    14821 gaaagaacgc caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa
    14881 atctacattg gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca
    14941 agatcagctg ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa
    15001 tgctgtagaa ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga
    15061 caccccaggg cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg
    15121 tgccgtttta gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta
    15181 tttagcgtta gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc
    15241 agcagatccc gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga
    15301 agctgtatta gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt
    15361 ggaatatgtt cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga
    15421 ctctatttat gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt
    15481 caaacccgga gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt
    15541 cggggtcttc tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta
    15601 tatcacagca agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc
    15661 agacaatcct gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg
    15721 tggtctttat ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact
    15781 gcaattgaat gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg
    15841 gttccgctgt ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg
    15901 ggaattcaat ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac
    15961 ggatggtacg actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca
    16021 ggatgtagaa gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc
    16081 agtaatggaa ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga
    16141 ttaccgagta aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga
    16201 caaattgaaa tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca
    16261 aaaaagcaag ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag
    16321 ctttatcgtt caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa
    16381 aaaactaatc ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat
    16441 cgttgcccgt tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg
    16501 agatgtttct cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa
    16561 acaaatcggc tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggatga
    16621 gcaagatagt gtgaaataac ttataatcaa tcgaaaaacc tattaaataa acctttttga
    16681 gtagatgcaa cactgaggat tatctgtata aaagatttta aactgtcatt tggttatcac
    16741 ggcgatacta aaattaacaa aattggaata tttttcttga agatttttag ctgtcagtat
    16801 atgatgaaag catctcatag gaggtgcttt tttcatggtt gataattcaa gtggaagggt
    16861 tcttaaatct ctaagaaaag agaaaaaact atctcaaaaa aagttagctg atttagcagg
    16921 tatttcacaa agtactttag ttaaatatga aaaagggtca agaaaaatcc ctaaagacgt
    16981 agataatact ctaagcaaga ttctgaatat agaaactcta ataaaagatg aggaagataa
    17041 gattgagatt ctcatcggta aactaattgc atacagagat atgaataagt tacttaacaa
    17101 agagttagct gataatatag gaattagtga agtattactc agttatgtgt taaatagaaa
    17161 gagaaatccg tcaaaagaaa tgcaaaaaaa aatcgatatt tttctattaa gtaatgaaaa
    17221 agaaattttg aaggagatta atcgtgatag tgagatattt agtttatcaa aagacgataa
    17281 aattgttatg ggaaaaagaa tacgagaagt ccgaaagaac cgggaagaga cgcttgaaaa
    17341 atttggtaaa aactttacca tctatacagg caaaaatgtt ataagtagat gggaaaaagg
    17401 tattaatatt ccagatattg aaaaactaat gaatattgcc tatcttggta aggtcactgt
    17461 tccttacctt atgtacggag aagattataa gaatatactt ccaaaagatg aaagagttag
    17521 cgattttaaa aagataaatt cttttagtat gggattaagg atgaggaaaa ttaggaaaga
    17581 ttattatcta gaaagggagg agtttggaaa gcttttttct ccttcaattt caaaatggtc
    17641 catagatagg tatgagaatg gtagagatat acccaatact aatcgaatta tccaatatgc
    17701 ttatatagga aatctttctt tagaattttt gatttacggg atataaaaat agtttacgtc
    17761 gatacttatt aagttatcga cgttttttat tatagaaaaa atttataaaa ggaggtttct
    17821 tgatatgata agaaacaaaa agggtttgtt gaaagttgct ttactggcag cttttttcta
    17881 agtaactttt tgacaagctc aatggcaaca caagcggaag aagtaataca agaactaact
    17941 gttatgaata acagctagat aaaatgggct atgtatgtta tccaacaatt tagtaatcac
    18001 tagaagccta gcgaaagttc gcttgataag ttatagatga gtattgattt gtagtgtttc
    18061 caaatcaaaa gggtacagag ccacatgaaa tatgttgtcc tctgcagcgt caggatggga
    18121 atccatacca tggagattcg gagatctgtg agggaatact tacgtggtta atatgtcagt
    18181 tgtgttggac gtagtaatgg tcatagtggg tagattgact gaattcttat atgagaaaat
    18241 gaaaccaaca agaagatagg gttatttata cagaattggg gatcttttct ggaaaagtaa
    18301 tcggataatg cgagattcgt acggtctttt ttaaatttct ctaaaaaaaa cggcatcgaa
    18361 tgggttgggt gattgataag caatgaatca tagactgata tagaataaaa aatgattcta
    18421 tttatctttt tacatctatc gttttttcaa gtagccgcaa attatatcag cattcaacaa
    18481 tttttgtata caagttctag taaaaggatc tgaagaaaaa gttggttgtt gtgaagataa
    18541 aaaaagaatc aggttttact gagtttttct tcaaaatttg tgttattagg ttaggacaaa
    18601 gataagaaga agtatgaaga tttaaaaaag gaatgaggaa aagatagtaa tttccgatta
    18661 ttggagtttt tttatttata aagaagaagc gaataagttg tgattcctag atttcgcgcg
    18721 tgattaatta atgagcaaaa aatgcggaaa gttttagtgt ttaatacacg aggacaaagt
    18781 ttcttaggta ttgaaggcac acgtgtaacg gttgatgggt ggtgtgattg tttcgaggtt
    18841 ggaaaacatg ttgaacttat ggcatttact gagctaaaag ataagaacaa cgtggaattt
    18901 ttcgagagtg atattggttg ggacgatcat gaagaagttt atggaaaatt tattttgcgg
    18961 aagggaaatt cttatacgaa tggggaagca taagtagcga tttgttagag atcattgata
    19021 ggattgaaat tgtcgggaat atttatgaga atctagagtt atttgaggtg atagaatgag
    19081 tgatggtttt gaacaagtat caataattaa aaatcaagtt cgggagattc ttagaaaaaa
    19141 aggctatttg gtagataggt attttgaagg agattacgaa acatgggtag gtgtctatgc
    19201 tcgtccagag aataaaccta cctatttaga tcctacgacg tcagaagatg cctatttaca
    19261 aaataggtat cgagtagatg gttttaagca agactttgcg gagtggtttg aatgggaaat
    19321 tgaaaacgga gaagtaaaag aagaataaga gtacttgttg tatcaaaata agccagtaga
    19381 acaaccaaac cagtagctag taatgacaat aatactgagg aatcggatca ttatggtatg
    19441 attgatattt ctcttccaga agatcttaag ccagatcctg taacaattgg caatagtgtc
    19501 atgatttttg attcattaga tgaagctatg tcatatggtt ggaaagaaat taaggatgaa
    19561 ggtagtaatt ggtatggcat gagatttggt gatggtgaag ttgccaaaaa agctggtatt
    19621 ccaattggga aatggacagt tgatttccac gaagcaggtt ctttttttgt tctgtaatta
    19681 tagtgggagg gtaaaatcat tgatgaaatg tgttcagata agagcgccag ataaaaatag
    19741 aagagacagt tcacgtcgtt atcacttttg gtaacggcgt tttgttttat tatataaaat
    19801 tctcaggaaa aggaggaatt cttgtgtatg caaaaacgaa aatgaattgg ttgaaagcac
    19861 tcgcattggt ggctttttct caattctgtt tcttggtggg aactgctcaa gcacatgcag
    19921 aagaaacgac ggctatacaa cctacgatgg aagccattgc ccaagtcgct tctgaagtga
    19981 cagaagtcca accagcagaa gccggggtcc cagctggtga agccactgtt gaacaggccc
    20041 ctgtgacaga agcgccaacg gtcaattcca aaacgactct cgatcagacg attggggaaa
    20101 cacaacaccc agggaatagt cctggtactt cacaaccagt tgaagaaact gcctcaatta
    20161 caacccctgc ttcagaaacg tccactctag aaggagaaaa agctgccgaa gcaacgcctt
    20221 ctgtgcagcc agagtcttct gagaaacaag cggaagaaaa gaaacgaata gaagaaacag
    20281 tggaacaacc agcggctact gctgacaaag aaggaaaaaa tgaggaaaac gttgaaaaaa
    20341 caatctcttc aaccgcagct agtcaaaatc aaccaacgaa accaaacaaa gaatctacgg
    20401 ctagtattga aaatcaaaag aaaaaagagc agaacattcc aaaaacaggt acacctacac
    20461 tgttagtaac agtaccaacc aatcgttcgg ttcgtgttcg ccgtgcaacc aatgcagctg
    20521 ttttaggtgg tcggaaaatt gattggggcg aaaatattgg tgttacttct agtccgaaca
    20581 atctaaactt cactttaaat tatatggttc gttttgagat taatggacaa gtggcgtttt
    20641 gtattgagcc tggtgtctca gccgcagaag gtcaagttta tacagctact gctttgaaaa
    20701 actatttgaa aaattcaaat gtacggaaaa aaacaagctt gatctcttat ttaggatatg
    20761 tcacaaataa agacaaatcc gatgaacaat acattgcggc acaatttatg atttgggaag
    20821 ctttgggcac aaaaatccca atcaaaaata tccgtgttga ttatgatcgt cgaaaagcag
    20881 aaattcaacg tttgattaat aactttggtg aaaaagcaac ttttaataat caaacacata
    20941 cggtaaaaat tggcgaaacg ttgaaagtaa agaataaaaa tgattttttg aaatatgtga
    21001 aagacattca aaaaccaaaa ggtgtcaatg tatctattca cggcaatgaa gtatggatta
    21061 cagtcacaaa agaggcaccg gataaagcaa aaattcaatt gaataaaatt aaagttcaag
    21121 gtgcgccatt agcctatcgt cgtccaggag ctcaaacaat tggtgtgtta catccaacag
    21181 aacctaatgc gtctgttatt aatttacaaa ttctaaaaca aggaaacatt cgtgtgttta
    21241 aagaagatgc ggaaactggt gtgaaagcac aaggtgcagc ttctttagaa aatgcggtct
    21301 atggtttgta tcaagcagat gggaaaaaga ttaaagaaat taccttgaaa aaaattaacg
    21361 gcaaagtaca ggcagaaatt aaagatcttg atcctggcaa atatgtttta aaagaaatca
    21421 aagcgccaaa aggttatgtg ctttctgatg aagcaattga aattgttgtt gaacctggaa
    21481 aaactgtcaa tgcgaccgca aaagatcaag tgatcaaagg aaccattgat ttagtgaaag
    21541 ttgctaataa agatttagtc gattcaacga atccagataa caaaccaaaa ttagctggca
    21601 ttgaaatcac tttgacgtca aaaacaactg gcaaagtggt gaaaaccgtt gtgacagaca
    21661 atgatggtta tgctactttt ggtaaaaata ccgttgtctt tgatacgtac attctttctg
    21721 aaaccaaagg aaaagaaggg tataaactat ttgaaccact tgaagtcaca atttctgaaa
    21781 atgggcaaac tttccattat gttttagaag ataaagtaat tgaacaacgt cttaaagtgg
    21841 tgaaagtgga tcaagaaaca ggaaaagtga ttccacttgc taatacagaa ttcaagattt
    21901 ttgatacttt agccaatcgt tatgtcacaa tggagattcc aaatgataca gaaacaacgg
    21961 atatctttaa aaccaatgaa aaaggctttt tcctaactaa cggtacactt acttatggaa
    22021 aagatcgcta tcgtttggaa gaaatcaaag cccccgtaaa ttacgttttg aatcaaaacc
    22081 cattagtgtt ctcgattacc aatgatacag aaccgatcaa agtgatccat tttgcgaacc
    22141 gtcaagcacg gaaaagtgta aaattattca agtatgaaga atggaacaag aagaaaacgc
    22201 cgctcgcagg tgtgaacttt accttgtata acggtaaaaa tcaagcaatt ggtgaatata
    22261 ccactgatca aaacggggaa attttagtga aaggtctagt tgccgatgac tattacttct
    22321 tggaaaatgc cccattagaa agtttccaac caaatacaga taaacaagca ttcacggttg
    22381 cattcaaaca aaatgcgcca gatgatgggg aaacgttagt ggtttctgta cgtaattact
    22441 tgattccacc aaaactagaa acacaagcgt caaataaagc cgatggtgaa aaagtggttg
    22501 atccattaga aacgatcgtc attaaagaca gtgtttctta taccaacttg tttgtcggaa
    22561 aagaatacac catttcagga gtattgatga acaaggcaac gaatgcgcca attttacaag
    22621 atggcaaaga aatcacaagt accttggcct ttattgcaga caaagtcaat ggcacaaaag
    22681 accttgaatt tgtggttaat gctagtgttc tgaaaggtca aacgatcgtt gtttttgaaa
    22741 aacttttccg tgatggcatt gaagttgcag cacatgcgga tatcaacgat gaagcccaaa
    22801 gtgtgaaagt gaataatcca accgttcata ccacggctgt ctttgaagac ggattgacag
    22861 tcgcagaccc aacaggattg tttaccttaa acgataccgt tgaaattacc gagattcttc
    22921 caggaaaagc atacttggtc aaagggatct taatggataa agcaaccggt gaacccttgc
    22981 ttgtgcatgg aaatacggta gaaacgagtg ttggctttat tgccaaagca gaaaatgaaa
    23041 cggtgatcgt accatttacc tttgatttga caggtataaa tggtcgtgaa atcgtcgtgt
    23101 ttgaaagtct ttttagaaat gatgaagaac tagcaaacca taaagacatc aacgacaaag
    23161 ggcaaacggt acgagtaact aacccaagtg tacgtacaac ggctacacat aaacccactg
    23221 gcttgaaaga ggttaatcca ttaacgaaag tcaccattac agacgctgtg aaataccacg
    23281 atttggtcgt tggaaaaaca tacacagtca acggtgttct aatggacaaa gtaacggaaa
    23341 aaccagtgct agtagatgga aaagaagtca caagtacctt aacgtttgtg gcagaaacaa
    23401 cggatggtac agtcaatcta gactttattc tagacgcacg tgcgttgcga ggcaaagaag
    23461 tcgtggtctt tgaagatctg tttagagaag gcgtattatt ggcttctcac gcagatatta
    23521 ccgacaatga ccaaaccatc aaaatcagta atccaaccat tacgacgaaa gcgacgaata
    23581 aaaatggcgg aaaagagatc ttggccttgc caaatcaaac tgtgcttgaa tgggtcaaag
    23641 tagacggtct tgcgattggt caaatctatc gcttaattgt tcaaggctac ttaacaccag
    23701 acggcacacc atttgaaggc actcatgcgg aaaaagtctt tgcggccgat aaagaaatga
    23761 tggaattctt atttgaattc ttagtggacg gtcgcaatct tgcaggaaaa ggcttgagtt
    23821 ttgcggaatg gctccaagcg aaaacggaag atgagaaaga aacgtttgaa gaagtggcga
    23881 aacacaatgt agatctaaca aacaaagatc aaacggttca aattgtggaa gcgccaaaac
    23941 caccgcaacc agtgaaacca gaacaacggg ttaaaccagc tactgttgta ccaatgttgc
    24001 caaaaacagg tgaacaaaca agtagtatac ttatggttct tggattagta gtattagtca
    24061 ttgctttagg tataacactt tataaagtac gtaaagccaa agattagtca tgaggaacaa
    24121 gcggttagga tttttaaccg cttgtttgat tgtaatgtag aaatatttag aatttatttt
    24181 ggtttttaag aggaggaaaa actttatggc agaaaaagca ttgcgtatga agaaagagtt
    24241 tttaggtcaa gtatttttat tgggttcacg ggaacctaat ccaccaaagg aaattaatgg
    24301 tgtgtctggg cgatatgttc aattacaagc agagtgttcg cgagaagaac agcctgcttt
    24361 ttttgtttat ctcacagctg aagaggataa aagattacga ttaccagaaa aagaggtgcg
    24421 tatttcattt gaagaagctt attttgtttt tcgtaaagaa cgagtgaata gtcgtgaaac
    24481 aaatgatatg ggacaaacag agcgtcgaac aactggctat caagatgttg gtgtcttttt
    24541 aactggtgta aaaattgggg aggttttgta agatgggatt aaaatttcca aataattatg
    24601 acattacaga agcattagat gaaacttgtt taggagccgt acgctttggc gcattagaag
    24661 agtctgatcg tgtatttgat aatgaaacag gcgattatac gggagaaatt cgatcacagc
    24721 gagcacaatt agtttttgga aatccagatg aaccaaagat tgcacgacga gttaaatttc
    24781 cagcagatgt agatttgact ggttttaaac gacgtgatcc tgtgaaatta attgatccag
    24841 aatatcatgt ggtatatatt ccagcacaag atggcaatcg tgaatattca gatttagtga
    24901 tttatgcgaa aggattagag aaagtagaag ctccaacaaa taatcctaat atgaaacaat
    24961 cacaagaaaa aggtttagat caaaaacaac caagtgttga ttctaaggaa aaagagaaaa
    25021 aataaggttt ttttgaaaga ataggaagga gtcttcctat tctttttatc taagatagaa
    25081 ggaaggggag attttgaatt cagataaaac caatcaaata ctttgagaga ttgtgcttgt
    25141 tatcaaaata tccaacaatt gattgagtat tttggagaca atccaacgat ttttgtacaa
    25201 acaaatgcat atatttgtgc ggttattggg cagtgtatgt ttttacgtga tcttgttcgt
    25261 ttttcattac cgtaccagtg gaaacaagat catcaggaag tgatttggat tcgtatagta
    25321 cgaatatgta atatgtgtgc acacccttat aaaaatcgat taaatctttt tcgattatgt
    25381 ttttttacta aatgtgatat tcctgtatta gttgcttttt gtcaaaagtt tttagcacag
    25441 gaaaagtaaa aacagaaagg aggaatgtga caattgaaag ctcatttttt caagtatcgt
    25501 ggtcggcgaa ttcgctattt caatcgttat ttatggtttt gctattatgg tttctttttg
    25561 tttccatttt tgattctgtt aggttggttt agttggaaag tattttatcc acgtttaagt
    25621 acgttttcga attggcaaat gtatcttatt cctggaataa gtattgtgtt gtatttgttg
    25681 ctggttagtg ggttaatttt agggttgatg cattggtcgc gactaaaaga aggatatttt
    25741 gcctctgttt attggcgtca gttacttgtt cgaatgttaa ttgacaatcg gtttgtttat
    25801 acaaagaaac aagttagtga gaaacaaaca agggaaaaaa tgagattacc aaaagtttat
    25861 ttttatcaaa agaaacaaga attagtggtt tcttttcctt tagatggtgg gcgttttcac
    25921 gaacgatttt taaaaatggg tcatttgtta tctgaagtat ttttgcgtga tttaattcgt
    25981 gaagaacgag aaaaagcccg aatcaattat gtattccttt cagattctgg tcggattcct
    26041 attgatcaat gtatagcaga aaatggacgt attcatttaa tgaaaacttt ggattgggta
    26101 tatgatcaat caccaaatat gttgattgct ggtgcaatcg gtggtgggaa aacctattta
    26161 ttgtattctt taattcaagc ttgtttaagt gttgggacag tagatatttg cgatggaaaa
    26221 gcggcagatt tagcagcatt aggtgacgtc gatgtattca aaggacatgt gttttacaaa
    26281 ataaatgaag aaatgattat ttgtttacga aatgcattaa aagaaatgaa tcaacggtat
    26341 gcctatatga aaaccatgaa agatcctaaa tatgaaccag ggaaaaatta tgcttattat
    26401 gggttaccac cgcattttat cttttttgat gaatgggcag ctttttatgg ttcgttggat
    26461 atggaagcga gaaatagagt cgataaattg attcagccat tagtattaaa gggtcgtcaa
    26521 gcagggatgt acattatttt agcaatgcaa cgaccagatg cagaatattt tccaagtggt
    26581 gttcgggata atttaacatt ccgtatttct gttggtcgat tgtctcccat tggttattta
    26641 atgacttttg gagaagattt taaaaataag ccattttaca acaaagatca acctggtcgt
    26701 ggatatgctt ttccaggatt tgatgtacct gtagagtttt ttgcgccatt tgtttacaaa
    26761 gattatcact ttatcaaaga atttgagaaa tttactgaaa tgattgtcta tgattttaca
    26821 gtaaaagagg atagtcaaga tcaaacgatg ggagatgata atcagttgtt acaagaacaa
    26881 ctagagcgcc aaatgtattt agcaaaagat ccaaaaggaa caagtttttc tgatgcgcat
    26941 attttgtaat caactaagga ggtgaagtta aatggcagag agttgttatt atagagtagc
    27001 tacagcaata cgaatgatta atccttctct ttcttccaga acgttttatg attggttgaa
    27061 tcgtattgaa caagtaactg attaccgctt tttgcgcaaa gaacgtgttt ttacaggaaa
    27121 agttattaat caagtattgc taacgaaaaa agatatagaa cgattgactc gattgtatca
    27181 ttatcgagta gatttagaag aggatttaac tttgtcaatt tatcgcgttt tttctcctga
    27241 aaaatatagt gaaataacaa aattagatca tttaattttg tagttattat ctatcgggag
    27301 ggctctagac gtagtctggt agccgcactc tgcctggcgg tgcgcattaa ggctgcgaag
    27361 catgggccta taatgcgcac tcgctagcag cgtgtgcagc tcacaaagag gtcgtgcgct
    27421 tgcgcacggc ctctttgtgt aaagcccccc gatatataat atggggggca gaaataacat
    27481 aacacataga aaaaagtttt ataaaccctt ttgtatcaag tgattatcta aggtcagcag
    27541 ttgtactact tttcccaaaa aacgaggggg actagttgat atatcggtgt ttctaaaggg
    27601 ttaaaaaaaa tcgattttgt tattttataa aaggagcgag aaaatgacac cgattgagct
    27661 aaaaagctac agaaagaagt taagtgcaat tcataatata agtggagata aaaagccgct
    27721 atcacaagca agatttgcgc aattgatggg ctgctcaaaa gattacatta gaaagatgga
    27781 acaagggaaa agaaaaatcc cagaggattt tgaacaacga atcaaggaac gctttcgatt
    27841 gtatatgaaa agcggaatag atcttcgagc aatgattgat tatttgcgtc tttccttttt
    27901 tgggacagac tacaaaacag tgattcaacg tgtattacga atggatctga cagaatttga
    27961 aacacgtcca acgggcttgt atctttttga ctgtctttct gttcgtggga atatctgggt
    28021 gttctggcat tcaaaacgaa ctgtaacaga aaatgtactt gtgcaatttt caggacaagg
    28081 ttgccgggaa tatgaggaag tcatgcgtga acaacaaaca gattggcgaa catggctata
    28141 ttccatttgg aaaaatcaag gaaagttgga agctggttat acacgtgtgc aatgtagccg
    28201 attcgatatt gcgttagatg aactttgggg attggaaacg gaagaacatt tcgatctctt
    28261 tgaattacta gaaaaacaaa aagcaggttt attagaaatg gactttaaga gattccagaa
    28321 tattggcggt tgttttttag atgatggcta ttttcgaagt gaaggattat ctttgtattt
    28381 tggatcgcga aagagtcctc tcttttttaa tttttatgag aaacgttttg aaattgcgaa
    28441 ccgagaaaaa atttcagaat tagaagcatt aacgaaatat ggtatttata atcgatatga
    28501 gttacggtta gccaatgaga aagcaacaca agcagtagaa gcatttattt taggtgattc
    28561 accgaaacgt ttaggagaga ttggcgttgg tttaattaat gcaaaactta ttgtttggga
    28621 tagactggaa agtgatgcac ggattgttga taaacgatgg gcaaaattat ttcgttcgat
    28681 ggaagaattg gattttacag taaagccaga aaaattctca ttagatcgtt cagaacgctg
    28741 gtttttgaaa caagtagctc cgacattaaa gttgttacaa gaagcagatc gtattttagg
    28801 aagagacttt gtacaagaag ggattgataa tgccgaacta aatgacgagc aacaaaacta
    28861 tctagaatgg atgggaacgt caggagggaa aattgcatga atgcatatac gacaacagaa
    28921 tggctgcgcc tgttggattt aaaggctgca atcgaagcat taaatgaaaa gatggttgat
    28981 ttgtcttatt ttcgttttcg agttccttat attgaacaag cggtaaaagc tggacgttat
    29041 caggaaaaag agaactggca agaaattgca cgcttattag aagttcgtaa gggatacgaa
    29101 caagaactcg aagagttgga atttagccga aggaaaggaa ggctagaatt cattcgcttt
    29161 tatcgatttt cattgccaat acctgcgatt ttagcggtta aaaaaggctg cgataaaatg
    29221 aagatatatg aaaactgtgt ggcagcactt agcagcgaaa aacctcttat ggaagaaatt
    29281 tcattggtga ctgtcacttg ggaaatgagt cgccagccaa cagaagaaca gacatatctt
    29341 tctttagaag agattgagat agaattagag gaaattggtc gctatgccac ttgttctacc
    29401 tattgtggat cggtcattag tattgctggt gtgattgtct aaatgaaaaa aattatgatt
    29461 tatgttggtg cgtattggtc acgtgatcca acagtattag aaaatccaga ggcggtacac
    29521 tattgcttgc gccaattatt ctatctgtat aaagaacgat tagaatcgtt gattcgtcag
    29581 ttaccatata ctgacagacg tttagatgaa cttcttctaa ggtatccagc aatgtataaa
    29641 cgacgaaaaa atagattgtt accagaagag tatcccattg aaaaacatga acttgaaggg
    29701 cgatttgtgg cttatttcta tgatgatgtt cgtatgcgct tagtagaaca acgaatggaa
    29761 atagaaaatg atcgctacta ttttatcaat tattgtaaga agcgtaagta tcaagtaact
    29821 gatgattttt atgattgtat tttacaagat ggggaagtga ttctatccaa aattgctcca
    29881 agttttcgtg aacttgtgcc gattgatttt ttgaaaaaat gtcatttgcg gattcttccg
    29941 taaaaaagag ctataaaagg aaagaaaagt agaagccaaa tcggtttctg cttttttatt
    30001 ttttttagga aggagaagat ggtatgtcac tggaattttt aggagagcca attgaaaagg
    30061 aagagttcgt ttcgcattat atggatttgt ttattcaggt gataaaaaac tttgtgcgat
    30121 tgatgaattt tatccacgcg aacaagacta tctagccgct gaacgaataa aaatcagttt
    30181 attatgggaa aaattcacag atgaaaataa ccgaccgcca gattatcgct atttatcaga
    30241 ttgtttaacg aataatgtgc tttctaaaga atacctattt cacaaacgtg agaaaaatca
    30301 aatgtcggtt gaacattttt gtgattgtta tattgcatta ttgcgtagtc aacagttatt
    30361 aacagtagaa cgatatggtg cagagtttgg ttatgtcatg caagctgagc gtcatagagc
    30421 tacaagatgc tatgaaaaag aagatcaagt gattgatggc tatgaagaat tatgtattcg
    30481 aaacaatcgc tatttgcaac aacgtttata tgaacaattg atgatggatt tttcaaatct
    30541 ctatagaggg tatgaatgtt cgatgattca agaaaggtag aagaaaaggg ggaattttta
    30601 tgtggcgatt attatttttg gtggtatgtg gtatggcatt gttctatgtg tatcgtgtct
    30661 atctctatcc acgaatggaa cgatggacaa gttggtatag agatccttta aaacaacgaa
    30721 aaggattgaa gcggtatcgt tatgaattac ttgttgaatt ttcggatggg cgacaagcac
    30781 agtattattt ttttgcaaac tatccaaaat acaacaccag tgaactattg attcaattat
    30841 tcaaaaataa tccatatgtt gggttagaag aaaacggacg ggagttcttt tatcgttcag
    30901 ataatattag tgcggcgtct atgaacaaaa cacgaattgc ataaaaaatt ggaggaataa
    30961 tcatgaaaaa aacaacatta gcagtcgtaa caacactagg aatttcatta ttttcgtttc
    31021 aattagggag ttcattggtt tatgcagatg aaactgtaga aacacctaag tcagaaattg
    31081 tagaaacatt gccaggggaa ggattaccag atgttgagac agagctaaat ggaggaacaa
    31141 cggaaaaacc agcagaacct gaacaattgc agtcagagaa acctgaaaaa gagccagtga
    31201 caccagaacc agagtttcct gaatcaactt taccgactga ttcgatcgaa gaagaaggtg
    31261 tgcatagttc aacaacaacg gattcaagta tagtcacaac tccggaacaa tcaggaacaa
    31321 ccagttcttc tgaaaagaat actgattcgt caagtacgga acagccaacg atgtcgtcta
    31381 ccagtgaaga acaaaaagaa gaaccgaaac aacctgttga aaaatcagaa gcaattccaa
    31441 aagaaaataa acctattgtt ccagcaaaag aagtcacagt atcggtaacc ccaagcggtg
    31501 aaattacgac gaatacttct caaggaatga gtgtaccgat tgtcactagt aatgtagagg
    31561 aattgaccca tattccaaca ccaaccaccc cattaaaagt agcaggtgat caaacaattg
    31621 ttggtgtaaa agatggtatt ccattaatcc aagatagtga agggaatttg gtaaaggatt
    31681 tgtcgattcc tgtaaagaaa cttccaagtg gcaatattga aatcaaaaca gcagatggaa
    31741 aaacaaaagt cttaccaaag actggtgaag aaatacatgt tgcactttct gttctaggag
    31801 gagtgttgac aagtattgct ggatttgttg gatataaaag aaagagaaaa ggaatagaaa
    31861 atgagggtaa ttgaatggac tatttttcag aaaaacaagc ttatcgttgt caatcgtacg
    31921 tactagtaaa aacatactag aaggagtagc ttatgaaaaa acgacaacta cggcggaaag
    31981 aaaaagttta tacagagaaa gtaccgaagc aaaagaaaat ccattcaaaa aaagcagtga
    32041 aatggatttg gtgtttggtt ttatttcttt gtttttcagg gggcttggca ttttttcaat
    32101 ccatgaaaac gaaggttcaa gtgtcgtttt tgaaagatga aatccaacaa ttacaaaaga
    32161 aagaagtaca aagtcggtca gaattaacgt acacacctga aattgaatcc tttatgaatc
    32221 gttttgttgc tttgtatatg aatacattcg ataatcatga acaagaacaa caacgtcaag
    32281 aaacattaaa aaaagagttt tatgcacaaa actttattca agaaccagga agtgtttcag
    32341 gtgttcgaaa attaactgct gcttcgttta ttggattaaa agaaattgaa accattccaa
    32401 ctgctagtta taaagtaact tatgatattc gctcgatgaa tccagagagt aagaaagaaa
    32461 caagtgtgac aatcacacaa gtactcaatc ttcccttttc tatttcaaac aagggctgtc
    32521 gtattattgc ttatccttat tttacggcac taccaaagaa tacagaaaaa caacgaatga
    32581 ttacttatga tgacacatta tacgaaacgg ttgatgcgtc ggaagcgaaa gatgttgaag
    32641 gatttgtgga agatttctta gataagtatg cgaaaaacaa tgcaaaagat atgtcataca
    32701 tgatgaaaga acctgaagga ctcaatcaaa cagctaaagt tgatcaagta gagatgaaag
    32761 tattgcgtta taaagaaacg aaaaaacctt ttattgtaaa agcaacggtc gtctttcgag
    32821 atggtgatac gtcactttta caaaaagaac aaatgacgat tgtcattacg aaaaaaacaa
    32881 atcagtatta tgttgaaaaa ttaacacata attggttgga taggaaagga gaagaacgga
    32941 aatgacactg aagggaattg tagattttat attaacgcaa ggtggctatt ttttaataat
    33001 ggtggttgtt tatctgggat tgcgaaattt tatgaaatca aaagtaggaa atgcattaat
    33061 atctatcgca gcaggtgccg cagtttattt cttcttaaat gatccaacac gtgtatttga
    33121 tgcgtttggt aaaatcattg aaaaattatt tatggattag gaggatagtg aagtggattg
    33181 ggaaccttat gattattcga ttgtttatgc ggaaccttat cgcgtacaac agattacaag
    33241 caattggcgt cttccatttc cagttgcatt agtcaatgca gcaacttttt tgttggtcgt
    33301 aatctttgaa tatttatttt tacgttcatt tattggttgg gtcatttctt tttttccacc
    33361 agcaggagtt gttttttata taggactccc ctatttatgt gtgttagcac tggcaaaact
    33421 aaaacctgat ggaaaaaaag tgcattttta tttatgggat tggttttgtt atatgattcg
    33481 tatgatgaat ggtgattgtg tgtattataa ctgggacaag gtatcaaaca atgcattgga
    33541 agaattactt tttgtgaata agtatgttta tttagaggat tagaaaaagg gacaatcaac
    33601 tggttgttcc ttttttattg ggaggtgaaa ttcattggtg cttgtaaaag aaccagtaaa
    33661 agcattcaag aaaaattgtt tgttaaatgg agaggattgt atctgggcct actatcatgt
    33721 ttttccacga acaatttcaa aaaacacacc aaaaaagaca caggaaataa aagaaatgat
    33781 ggctcgttta ctgttgcttt tagcagatta tgaagatgtg gagttggtga tgtatccacg
    33841 aacaatgaat ttacggacga agtttgacac attaggaaag ggattttctc ccgatagtca
    33901 agcaattggt gcgtattatg caaaagaaac agtgacattg ttggaacgag aaggtcaagt
    33961 ctatcgtcct tcatttattt taggaatcaa agtcaaagaa aaagcaacgg tggagagttt
    34021 tcatgatgta ataaatctaa tctggttaga tttagcagga agtttagcac gcttatttgg
    34081 attagaagta gatgatttgg aaactttgtt ttctgaaagt gatccttatg taaaagaagt
    34141 gacacaatta ctttatagct tttctattcg tcccttagta aaaaaagagc tgttgtatct
    34201 caatcgtctg caatatattc gaggaatggc tcacaaagta gaagaacaaa ataattggga
    34261 agtactgaaa gacaatcaag gaacgtatgt acgtataccg aaggaacata aaggccttct
    34321 tcaattagaa gatgagttag ggagtagtta tttagcattg ttgcctattg gggaaacacc
    34381 tgaaaatatg agaaattgtg atctatttca atttgctcag gatctttctt tcccagcaga
    34441 attgcgaatg aaaattcact atccacgctt gaaaggacag ggaggtattg gtttaaaact
    34501 gaattggcta tcggaacggt tcaaaacaga gcataaggaa ttggttcaaa atgatgaaga
    34561 acctagtgaa cgcttattaa ctgttcgagg attagtaaaa catttaaagc aacgagtaga
    34621 taatgggcaa cctgtcgtcg attggatcgc atgttttatt gtatacggtc aaacggttga
    34681 agaagttcgt caaaatagtg caacggttat tcaacaactt catacgatta tccatatcca
    34741 ccgtgcaaaa aatgatcaac gagaattgtt ctataaatgt ttattagggc aaccattggg
    34801 acggcagtta cattggaagc accgaacaac tgtagaagca gttggcgaat ggttgttttc
    34861 agcagtatca gaattaggta tggaatccgg ctggtatatt ggtcggcaag atacattagg
    34921 ggatggtggt tctcgaggag aaaaaacacc attagaagaa ttaatttatg cgtcaaatcg
    34981 ttttgtcttt ttgaatcttc tagccattgc ggaaggaatc aaaggtgcgt tgtttgatgc
    35041 gcctcatgta gcagtaactg gaaaaaccgg taaagggaag acctttttag ttggcttgct
    35101 ctttttgtat agttcgttta tgaatgtaca aagtttattt gtggatccca aaggagaaaa
    35161 gcgcaaatgg tttacgaaac tcattcaaga cccgtattat cagaaacatt accctttgtt
    35221 tgtagaacat ttgcaaaaaa tgagttatgt cacgttagat gctacaaatc cccgtaatta
    35281 tggtgtatta gatccaattg tttgtttatc ccgagtagat gcgaaacaag tagcccagga
    35341 tatgattgat gagttaagtc cgttaggtac caatcacctt ttaaaagggg cggtattaaa
    35401 gggaatccaa gaagttttag ttcaaaaaga agccggacaa caagttggct tgatgcatgt
    35461 ggtcgactat ttagaacaaa tggaacaaaa agaggttcga gaatatggtg agtttttacg
    35521 cttgacggta gaagattcga ttttacgttt aggattttct tatggagaaa acccgggatt
    35581 agactttaat gccaaaacaa cggttttaga gatccaagat ttgaaattac caaatgacaa
    35641 tgtccgacca gaactatata cagatgcgga tcgaaagtct ttgtgtttga tgattagttt
    35701 aggtcgtttt tgtgaaatgt ttggaaaacg agatagtagt aagaaaacag cgatttattt
    35761 tacagaagca tgggtattta ataattcgaa cgcaggacgt agtattattg cagcaatggc
    35821 acgtgtgggg cgttcccaga tgaatcaatt agtgttagat acacaattta ttggtgactt
    35881 aggttcagat aaagaaaaag ggaattttgg tgtcgtcttt gcttttgatg aagacagtga
    35941 gcgagaaaag attttgaatc atttacgttt ggaagtaaat gaagcgaact tagctgaaat
    36001 gagccacatg attaaaggac agtgttggat gttagatccc tatggacgtg taggaaaact
    36061 caatgtacac agtttgtttg aagaaatgac cgctgcatta caaacaacgg aaaaaacaag
    36121 tcactctcaa gtagaagaac aggatacgtt aatcatgcaa tagaaatgag gtgaaacaat
    36181 ggcttatata aagcataagg aaaagaaatg gtgcgttttg ttttttttaa cgattggatt
    36241 cggactgctt tttgttggtg gattggtgta tgcggcagaa aagcaggtgg atcaatcgat
    36301 ctatgagatt gggaaatttc atattgatac cttttcttct tatttatctg atagtgaatt
    36361 ttcattaggc aaaatgttat ttggggcaaa tggcacagat tttctaaaaa tgatgacgaa
    36421 tatgttcttt agtttaagta aattgatttg gcaaggcctt gattttgtca ttgagaagct
    36481 ctatgaagga gctgcaatga acgagttaat tcaccagttt ttcacttttt cggatactat
    36541 ttattcaaaa atatacgatc ctattggtgc ggggattgtc gtcttgtatg tgttatatct
    36601 atttactctt cgtatattta gagggaaaca ttacgcaaaa atggtgttta ttcgatttct
    36661 cttcgttgtc attttttcaa tggtttggtt tggctatggt agtaacgtac ctagtaaagg
    36721 agaagctttt accaaagggt taaatgattt gtccgtggag gtagaaggct ttattttttc
    36781 tgctacaaat agtctagatg aattggcgac aaaagatgtc aatggtgttt tagtgccaac
    36841 gacacaaaag gaagccattc aaaaagtgcg ccaactttat tataaagcgg ctgttgttga
    36901 tccgtatata ttgttaaatt atgggacaac aaatttagaa gaattgtatc aagcagagat
    36961 tgaaccacaa gaatttctag ccaaggaagc gtcaagtgaa gaattatctg atattgagac
    37021 aaaagtgacc actgctgcag gagaagatag agggaaaaag aaggcttatc gtgtctatat
    37081 tcaaccaact aaagctgttt ataaagcagt cgttggttct atggtgccat tgcttaatct
    37141 aagtattgga ttaccacttg tagtgattgg catgattcgt tttatctttc aaatgttggt
    37201 gttgtgttta ttacttgtga ttcctttttc attactcgct tcttttgttc cagtatttga
    37261 ttatatgctc atgaatacct taaagtcctt tttgggcttg atgtttcaaa aatcttttta
    37321 cagtgtgatt attttggtgg catttttgat ctataacata gtagacgatt taattccaat
    37381 gaatactgtt tttggttttt taggaaatat gtttgtcaaa gcactcctat caattttact
    37441 cattgtaaaa cggaaagatt tattaaagaa actagggtta ggagctgcag atcaaactat
    37501 aaacaacacg aaaaacatag caagtcattc tttgtctcaa atgaaacaac tgcctggtca
    37561 actatcaact gctggacaaa atttggcaat caaaggtgca ggattagcag ggaatgtgta
    37621 tcgagcaacg aatccactcg cttctgcagc gatacatgga tttcaaaagt ttcaacaaaa
    37681 acgtacacca caagaatcgg taaaagggag gcaagtggtt aatccctttg aactaggtgg
    37741 gcgttcaaat caagccttgc cttatagtgg tcgtcatgtt cgttatcatg gggtagagcc
    37801 tgaaatgtct tctaccaaat cgaaaacaga gtggaattct ggtctttcaa atggtttgat
    37861 tccaaattcg tccatgaatt cagtaagacc aaaacaacag gtacaatcat tggcacaatt
    37921 gagtaagcaa caagggaata atcaaagaaa accattgtta aataaaggta tcgcttttgt
    37981 tggtcaatcg attccgttgc cttctggaaa agtacccgtg ccacaattaa cagctgctgg
    38041 ttctctttca caacagattc gccaagtgaa aaatcaggcg aaaaatggga gttatccagc
    38101 aatgcagcag ttattgaacc aatactctgt tcctaattcg tcggtagcca ctctatcagg
    38161 aaatcgttct gtgcaacaac cggggcaacg ttcgtctgaa agtaaaccgt cgatttccgc
    38221 accaaaatcg ttacaacctg cattttcttc tttagataag ccaacagagc aatcggaaaa
    38281 aatagaaaag gatcctaaaa cactgccgat tttggacgca aaaccaggaa ctgattttac
    38341 tttaccaaaa ggacgaacag tccaaaaacc attatcgaag aaaggatgag gaagaaatga
    38401 agaaagggtg tctaggttgt cttcttttct ttattcttcc cttttttcta ttgccattct
    38461 ttttttcagg agaactttct gagcatggag gagaagctac aggtctagct gtcgctttta
    38521 atggggaata taccgaagga ttaccgattt tttcagagat taaaggacgt ggtccaatca
    38581 cagatgaaca tgcgcaagca gcagttggtg cagctgtaaa gtatcagtta ttaccgagtg
    38641 tgattctttc tcaattaggc tatgaatcag cttatggaca gtcacaatct ggaagaaacg
    38701 atacgaactt ttttgggatt acctggtttc ctggttgtcc ctacccacaa ggttctgcac
    38761 gtggaattgg tggttctgaa ggtggtttct atatgaagtt tccgaactta aaagcttgtt
    38821 atgactatta tggctttatg gtggcgagcc aaagcaactt taatcaatgt gtgggcaata
    38881 aagatcctgg aagttgttta ttgattctag gtcgtggagg ctatgcggca gcaggtatta
    38941 ccgaagggag tccgtattat cgaggagcaa tgagtatcat aacgtcctat aacttaacgg
    39001 aatatgatac ttttgcgatc aagcattgga atagcctacc aacaaaaaaa acttcgctta
    39061 ttgcaggaaa aagtaaaggc aatgcgaagg ttttggatcg gatcattgga cgatccgtaa
    39121 ataatgggga atgttatggt ggtacggcat ggtatgtatc tcaattaggt ggcccacagt
    39181 taatgggttc tggacacatg tttgcgatgt taatagggga agattataac tggacaacct
    39241 atggttggca agtaatcatg aacccaaaac caagtgattt acaagcagga gatgtggtga
    39301 attggcaagc aggtggtgca ttgtctcctg gaatctatgg tcacaccgga attataacag
    39361 atgtttcata tggcggacaa catttctcta gtgtggaaca gaatgccgag caaggacgga
    39421 ttatggctcg gtatcaacgc acgtacaatc aaacaaaaat tcgtagtctg gttcgtaagg
    39481 tgaaataaga aaggcagtga ctagaaacgt gaaaaaaggg ataattttag tatttagttg
    39541 gttatgtttg ataggtctta ctggttgtca ttggttttct tctgaaaaac aaaaaacaca
    39601 attgagaacg acagaagaaa caatcgaagc agtaaaagaa acaagtggaa ctcttcaaag
    39661 tactgaaagt aacagtcaaa ttggttcatt agaagacgag acagcgcaac aaatgcaaat
    39721 agatgaaatg acaaaaaatt tcttaaaaga ttttgctaga aaatggagta atttcacaga
    39781 tgtttataaa cgcaatcaag ctgtgaggga ctatatgaca gatcgttgta ttaaagacaa
    39841 tagtattgat gtagacccgc atgtacaaat gaaaacaact gggaaacttt accaggtgac
    39901 acaagatata gagaatccgg atcaatattt gttatttgga gaagaaacaa tgaataaggt
    39961 tactcaattt gtattacttc aattgaaagt agatcaagaa gctcaaaaga ttgatgcgat
    40021 caagatttat tatgtacgga gtgcgtatta aaataaaaaa tgaacactca tatttattat
    40081 acaccgaaga aatagaaaaa aacagactgt ttttttaaga aaaactatgt cattctttac
    40141 ttgaaaaaag ttgttatagg acagaaagga taaaacaaaa acggaaaaaa ta
//
LOCUS       DAFFSV010000012        36930 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFFSV010000012 DAFFSV010000000
VERSION     DAFFSV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.012"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..259)
                     /locus_tag="LR110_000543"
     CDS             complement(<1..259)
                     /locus_tag="LR110_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL4821546.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQV"
     gene            complement(375..941)
                     /locus_tag="LR110_000544"
     CDS             complement(375..941)
                     /locus_tag="LR110_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HBL4821547.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(1077..1730)
                     /locus_tag="LR110_000545"
     CDS             complement(1077..1730)
                     /locus_tag="LR110_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821548.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(1780..2208)
                     /locus_tag="LR110_000546"
     CDS             complement(1780..2208)
                     /locus_tag="LR110_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL4821549.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(2213..2587)
                     /locus_tag="LR110_000547"
     CDS             complement(2213..2587)
                     /locus_tag="LR110_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4821550.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            2891..3076
                     /locus_tag="LR110_000548"
     CDS             2891..3076
                     /locus_tag="LR110_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4821551.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            3089..3337
                     /locus_tag="LR110_000549"
     CDS             3089..3337
                     /locus_tag="LR110_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821552.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            3368..3538
                     /locus_tag="LR110_000550"
     CDS             3368..3538
                     /locus_tag="LR110_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821553.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(3640..4098)
                     /locus_tag="LR110_000551"
     CDS             complement(3640..4098)
                     /locus_tag="LR110_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821554.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQSISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            4266..4583
                     /locus_tag="LR110_000552"
     CDS             4266..4583
                     /locus_tag="LR110_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821555.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            4516..4995
                     /locus_tag="LR110_000553"
     CDS             4516..4995
                     /locus_tag="LR110_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBL4821556.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            5012..5188
                     /locus_tag="LR110_000554"
     CDS             5012..5188
                     /locus_tag="LR110_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821557.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            5444..5875
                     /locus_tag="LR110_000555"
     CDS             5444..5875
                     /locus_tag="LR110_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821558.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            5881..6198
                     /locus_tag="LR110_000556"
     CDS             5881..6198
                     /locus_tag="LR110_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HBL4821559.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            6317..7003
                     /locus_tag="LR110_000557"
     CDS             6317..7003
                     /locus_tag="LR110_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4821560.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            6978..8327
                     /locus_tag="LR110_000558"
     CDS             6978..8327
                     /locus_tag="LR110_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL4821561.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            8318..8791
                     /locus_tag="LR110_000559"
     CDS             8318..8791
                     /locus_tag="LR110_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL4821562.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            8766..9293
                     /locus_tag="LR110_000560"
     CDS             8766..9293
                     /locus_tag="LR110_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HBL4821563.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            9308..11620
                     /locus_tag="LR110_000561"
     CDS             9308..11620
                     /locus_tag="LR110_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBL4821564.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            11867..12184
                     /locus_tag="LR110_000562"
     CDS             11867..12184
                     /locus_tag="LR110_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBL4821565.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            12181..12342
                     /locus_tag="LR110_000563"
     CDS             12181..12342
                     /locus_tag="LR110_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL4821566.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            12345..12644
                     /locus_tag="LR110_000564"
     CDS             12345..12644
                     /locus_tag="LR110_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821567.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            12644..12958
                     /locus_tag="LR110_000565"
     CDS             12644..12958
                     /locus_tag="LR110_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL4821568.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            12951..13481
                     /locus_tag="LR110_000566"
     CDS             12951..13481
                     /locus_tag="LR110_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBL4821569.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            13478..13747
                     /locus_tag="LR110_000567"
     CDS             13478..13747
                     /locus_tag="LR110_000567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821570.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            13677..13880
                     /locus_tag="LR110_000568"
     CDS             13677..13880
                     /locus_tag="LR110_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBL4821571.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            13877..14164
                     /locus_tag="LR110_000569"
     CDS             13877..14164
                     /locus_tag="LR110_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821572.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            14651..15064
                     /locus_tag="LR110_000570"
     CDS             14651..15064
                     /locus_tag="LR110_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL4821573.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15684..15848
                     /locus_tag="LR110_000571"
     CDS             15684..15848
                     /locus_tag="LR110_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821574.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            16260..16805
                     /locus_tag="LR110_000572"
     CDS             16260..16805
                     /locus_tag="LR110_000572"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4821575.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            16806..17123
                     /locus_tag="LR110_000573"
     CDS             16806..17123
                     /locus_tag="LR110_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL4821576.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(17280..17444)
                     /locus_tag="LR110_000574"
     CDS             complement(17280..17444)
                     /locus_tag="LR110_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HBL4821577.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            17546..18067
                     /locus_tag="LR110_000575"
     CDS             17546..18067
                     /locus_tag="LR110_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBL4821578.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            18054..18527
                     /locus_tag="LR110_000576"
     CDS             18054..18527
                     /locus_tag="LR110_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBL4821579.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(18534..18692)
                     /locus_tag="LR110_000577"
     CDS             complement(18534..18692)
                     /locus_tag="LR110_000577"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HBL4821580.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            18785..19546
                     /locus_tag="LR110_000578"
     CDS             18785..19546
                     /locus_tag="LR110_000578"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL4821581.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            19766..21085
                     /locus_tag="LR110_000579"
     CDS             19766..21085
                     /locus_tag="LR110_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL4821582.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            21101..22321
                     /locus_tag="LR110_000580"
     CDS             21101..22321
                     /locus_tag="LR110_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL4821583.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            22356..23033
                     /locus_tag="LR110_000581"
     CDS             22356..23033
                     /locus_tag="LR110_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBL4821584.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            23030..24223
                     /locus_tag="LR110_000582"
     CDS             23030..24223
                     /locus_tag="LR110_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL4821585.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            24238..24564
                     /locus_tag="LR110_000583"
     CDS             24238..24564
                     /locus_tag="LR110_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBL4821586.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            24568..24903
                     /locus_tag="LR110_000584"
     CDS             24568..24903
                     /locus_tag="LR110_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL4821587.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            24900..25295
                     /locus_tag="LR110_000585"
     CDS             24900..25295
                     /locus_tag="LR110_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821588.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            25292..25663
                     /locus_tag="LR110_000586"
     CDS             25292..25663
                     /locus_tag="LR110_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821589.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            25679..26239
                     /locus_tag="LR110_000587"
     CDS             25679..26239
                     /locus_tag="LR110_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4821590.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            26364..26675
                     /locus_tag="LR110_000588"
     CDS             26364..26675
                     /locus_tag="LR110_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821591.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            26699..26890
                     /locus_tag="LR110_000589"
     CDS             26699..26890
                     /locus_tag="LR110_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821592.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            26890..30738
                     /locus_tag="LR110_000590"
     CDS             26890..30738
                     /locus_tag="LR110_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBL4821593.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            30739..31611
                     /locus_tag="LR110_000591"
     CDS             30739..31611
                     /locus_tag="LR110_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HBL4821594.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            31608..32657
                     /locus_tag="LR110_000592"
     CDS             31608..32657
                     /locus_tag="LR110_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821595.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            32661..33485
                     /locus_tag="LR110_000593"
     CDS             32661..33485
                     /locus_tag="LR110_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821596.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            33503..35563
                     /locus_tag="LR110_000594"
     CDS             33503..35563
                     /locus_tag="LR110_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBL4821597.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            35580..35729
                     /locus_tag="LR110_000595"
     CDS             35580..35729
                     /locus_tag="LR110_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821598.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            35726..36172
                     /locus_tag="LR110_000596"
     CDS             35726..36172
                     /locus_tag="LR110_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821599.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            36174..36311
                     /locus_tag="LR110_000597"
     CDS             36174..36311
                     /locus_tag="LR110_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL4821600.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            36346..36588
                     /locus_tag="LR110_000598"
     CDS             36346..36588
                     /locus_tag="LR110_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBL4821601.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            36604..36801
                     /locus_tag="LR110_000599"
     CDS             36604..36801
                     /locus_tag="LR110_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL4821602.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            36812..>36930
                     /locus_tag="LR110_000600"
     CDS             36812..>36930
                     /locus_tag="LR110_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL4821603.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSS"
BASE COUNT        12739 a         6212 c         8034 g         9945 t
ORIGIN      
        1 taacttgtct tttgtatata actaaccatt cttcaacaag ctcatgaaat gttagatcag
       61 gcttatgaag tgttttttcg tttaatttta tatcaatttt tttatttaac tctagcaatg
      121 ccactttctg agtttctcga gatttatttt tataagtaat gctcactttt tttctttttc
      181 tagtttttgg atcaatatat cgctcattat atttatatac tttttttccg tttttatcag
      241 ttttagtttc gatccacatt gttttcatct cctatttgtt gctataatag gaatagataa
      301 gtaagccgat tatagcaggt ttatttttca tcacgtccac aaactttggc gagggagggg
      361 cgtgtttttg ttagttattg tggtaggtta tctaaagcgt attgtgcttg ctcatttgta
      421 aaaccttcaa atataagttg atcgtacaat tgcgaatctg acattgatgc ccaatcatcg
      481 taactttccg ctttagccaa agcttgttcg ttgtaatcga catcaacatt agccagcgca
      541 taatcgattg catcacttgg atattcttca aactctagtt gttcacgcaa accttgttca
      601 gacataccag cccaacctag atagctttca gctttgctca atgcatttct gtattcacga
      661 ggaacacttt cttcttgagt aacttcttct atagtagttt cagtagtttc agtttcttct
      721 tcaacgactg tagattctgc taccgattct gcagaagaca cttctgtcac gctttcttta
      781 gttgtttcag cgacggtatt tttagcatca tcactgcctc cttgcgaacc tatagcaata
      841 aacaccacaa caaccgctaa caaccaaaac catacccgtt tataaaacgg tttcttaact
      901 ttatacattt tcccatcttg acccataact ttttttgcca tttaaatatc cctcgcttct
      961 tgttataata tatttgtgat ctcagaaatg aggtatgagt ccgtgttgca gcacgggctt
     1021 ttttctttat aactttttta gagattatag gcaaaatagt agggcataaa aatgtattat
     1081 tgaattccgt atttagaaaa tcctaattga acttcaccgg aagtcttttg ctgtgttgta
     1141 cgcaatgctt cttcttcaga cattccattc tgtactttcc atgcaacagg cgacatcccg
     1201 tatttgttaa caaaatcagt aagtgataaa gtgtcagcgt cttgctgagc gcttgtttgt
     1261 tggtcttctg gattctgttg agatgctgct tgttgttctt tctgatcttg actgataata
     1321 ttgccagcat catctgtagt caatccattt tcataaaggg ccacgccgaa agcttcccac
     1381 tctttgttgg accaatttgc acgatcagct ggagttgact gtaaagtgcg ttgtttcatc
     1441 tgttcatatg tttcttcttg aggtgcggtt tggattgtac tctgactgga gcttataact
     1501 gttgggctag gttccgccgt agcttggttg gagcttgtaa ctgttgaact agaatctgtt
     1561 ttagatgtag atttgctagt agatgaactg gtttcagttg tttcttttgt tttacttatt
     1621 tttgtttctt ggttagaagt ggtatctgtt gattcagctt ttttattatt tgaacaagct
     1681 gaaagtagca gagcagtact taacaacaac ataacgctaa cttttttcat tttataattc
     1741 ctctttctcg ttgtaatatg tgtgctaaca cgggcttttt tatataagaa aacgataagc
     1801 gctttctgga agcccgtaaa gattctttaa ttcctcgatt tttttaggat attgatcatt
     1861 atcttcttta taaagagaaa caatgagatt agcagcaaag caattagctt cgctttcaga
     1921 tttgcttcta gatgttcttg ttgatacata atagctggat aagccacgat gaaaaatagc
     1981 gtgacctaat tcgtgagcgc aaatgtagaa tctttcctca gagtctcgca gttcatcatt
     2041 taaaaagatt atcgcacgat ctctaatttc ttgaaactgc cccttgggat tctcgataaa
     2101 aggaacgtat tgaattttaa tgcccatctt ttcacaaata taaaaaggat tagcggactg
     2161 gtatttccgc ttcaactcct cgactaaatt aatcgtatcc atctccataa gctcacatct
     2221 ttttgccttt ttctttgtct tctttcacaa tatcccagaa agtcgctatc aggatatctt
     2281 ttacgcgctg tatttgttcg ggtgtcaatg tttccccacc ataagacata ttaacatttg
     2341 agtctagtag tttatcaagt tcaaccactt cctcttttgt agcccacttg ggaacattat
     2401 tatttcccaa taaataatca gttgtgacac caaaataatc agcaactttc tttaagttct
     2461 cagattttgg cgaggcttta tcccatcttc ttatttgtcc attagagatg cccacctgtc
     2521 tttctacttc tgctatagtc acatgctttt cgtctgctaa ttctttaatc ttagtaacta
     2581 aactcattat tatcaacctt tcaaagctga aaagaaaata aatagcttaa aagttatatt
     2641 tttggttgac aattagcttt taagctagta tatttaattc gtaagctaaa ttgttagcta
     2701 aataagagca acaaaaaact ctactaattt aaaacattct ctcggtcgcc aaacttagaa
     2761 atgttatttt agaggctttt tataagtctt atttaactat gtattcataa tagcttaaaa
     2821 gctaataggt gtcaacgatt tagctaattt tttagcttac aaattatttg tttagaaagg
     2881 agctattttt atgtctgaga atttagactt aaaaattcga gcggagatga gaaaaagaag
     2941 aatgactttc aaagaactag ctgcgcttat cggtatttca ggagcttatt tatcagatat
     3001 tctaaacggc aatcgtgatg gaaagaaagc acaacagcat atcgaaacag tgaaaaaaat
     3061 attggacatc cgataggggg ggtaggcgat gacaaaacta aaaaaacaag attttgtaaa
     3121 aaaatataat tattctccat ctacttatca acgtcgaatg tcggaactaa aaaatacagc
     3181 aattttctca gcggcgtatg aacgggtcac aggacaagaa gtttggatca atacagaatt
     3241 atacgataaa tttttgtctt tcaaatccta taacaggtta cgcacaagaa aggtaacacc
     3301 taaagaattt atcgagaagc atttagttga tttataaaaa acagaacatt ttgagaggtg
     3361 aaggttaatg ggtaaattca acagagcatt agtattcagc gcaccgctaa tcgtctatgc
     3421 tttagggctt tggggaagta gacaagcgtt gataggaacg attgtgtaca tggtttggat
     3481 ttttataggg ctggatgaag ctgagtacaa aacaaaaaag ccagtcggga gggactgact
     3541 aatgagtaag gacgatattg tatttctgtt aattatgttt tgtgggattg gatcaataat
     3601 aattaatttg tatttgatta ttaaagaaaa atttttcaat taaaatgagt cttttagctt
     3661 taacttaaat tttttttgat aattagctgt tcttatgacc attacaagtt tttcaccagg
     3721 taatatatct ttcatatcta aaacttctga aaaagcgact gagtagccag gagagattga
     3781 ttgtgccaac ggtataggag ataaaaggct acttttatga atgcttccca tatcaaaacc
     3841 attggcgtga taatgtgaac aattaatttt cttaccgttg taaagatacg tacattcgat
     3901 aattgagttt ggcatttttg atgggtttgc aattacgcca gtaagaatca ttcgattact
     3961 tctatcatgc ttaactaaaa aataggagag cgtaattttt cttttatctt taaataagtt
     4021 atagagggat aaaaaaaatc ctgaaataga aatgattaaa gtaatagcga cttggtaatc
     4081 gctcaaaatt tttatcaagt aattcaccac ctttttactc aaattatatc aaaaaggaga
     4141 gaagaaataa tgcaagaatt agtaattttg aaaaataaaa aaagacacga acgccggcaa
     4201 gcatgatcgt gtcagggaaa taaactatct ttctatattt taccacagaa aggaatgaac
     4261 cgtaaatgct aattgcaacg gatacactag accgcatctt tttaaaagac gaatacaaac
     4321 tgcgcaatat agatgcgtca ggaattttag tatttgatct ttatgacaat ggaaaaattg
     4381 gtatctatca agcaagtgat attgaagaaa caaaccttgc tttcgaacaa attgatgatt
     4441 ctgtggaatt ggatttagat gaggcaatcc tagcttttga acaaattgca aaattattaa
     4501 aggaggcaca aaagaatggc aactctttac caactcagcg agtcatatat caaagtcctg
     4561 gaactggcag aacaattgga tgaagaaatt cttcgcgata ctctcgattc gattaatgaa
     4621 gcgatcgagt ataaggcaga aaacttagca aaaatagtta aagaagtaga agggaaagct
     4681 gagttaatcg atagtgaaat taaacgtctg caggaacgta agacatcgct tttgaacaat
     4741 gctaagagta tcaagcacta tttacaagag gaaatggaaa agactggtaa aacgaagatt
     4801 aaaggtgaat tattcaacat tggaattcaa aataatccag tatcggtcaa tgtaatcaat
     4861 gagaacttaa ttccaaaagg attctttacc cctgtgcctc ccaaattgga taaaaagcaa
     4921 ttgaaggagg aactgaagca cggagatatt cctggtgctg aactcgtcca aacaaaaggt
     4981 ttgaggataa gatgatgaag gaggagaaat aatggaacac gaaaacgtaa aagacgcttt
     5041 gaaagctgct atcgaaatcg cggaagctaa aggaataaag gttgacggca aaccagcaac
     5101 tgtccatgac attcaaaatt tgacgaaaga acatctttat tttatcgccg acttgcttgg
     5161 tttgtcggaa ttgtatttag acaaataacc ccacacggaa ggagtggcta gggtggttgt
     5221 attacaagta atagtctgcg taacattcat ttggtcgtgc ataccggttt gtatatatat
     5281 catgaagaag cttcgccctg agtcttacaa attatacgca atttacagca taacagtagc
     5341 tcttgcattt acattgtttg caatttctag gcttccgttt taatcgtggg aatgaatttt
     5401 taatgaacta aattatctat aaagcaaaaa aggagaaaca acattgaaaa acaaactagc
     5461 aaaaacaaca gcaatcatcg gactagcact aggtagcggc gttatcggct acgcagcaag
     5521 caatgcgttt caagatttgg acacgatcaa agcaaatttc aacacagtcc tacaatacgg
     5581 tcaaacgaaa tcgcaacgtg tgtcagaact tgaatcacag ctatccaaca acactcgcac
     5641 acaggagcag ctgaaagccg aaattgagca aatcaaatcg gacaagcaga aggaaattga
     5701 agccaagcaa cgagaagtcg aacaaaagca acaggagatc gctacaaagc aacaggaagc
     5761 cgatagcttg cgccaacaac tatccacagt gcaaaacgac aaggagcagt tagaacagcg
     5821 cgtgagcgag ttacggcagt atacggatca aaaggtaggg gagttgggga aatgaaagag
     5881 ttgactattt atgacccaaa tatttcatgg gctaaacaca caattaaagt ttcttttatg
     5941 atttggggtt acaaaggata tgttacttac aaagtgggcg gtaacactaa gggtttatca
     6001 ttaatagcta ttgattcaga tgatctatat gatgctaatt tcgaggataa cccagttaat
     6061 ttccgtgacc ttgatgaaga ttggttctct atggaactta ctaacgataa aggcgacagt
     6121 acacttgtag aagatgaatt cgacagacta ggtgattata ttgtcggtgt tgaaattatt
     6181 gcgcacgaac cagagtagga gagtgaatac aaaatgatta aaagaatcac tatatcagtt
     6241 gaagaagata gtggatcaac aatgcagcat accgacaatc gaaaaacttt tttaaccaat
     6301 agaaaggaga attttgatgc agattaagaa ggcgacggat atcgaacaaa ccaagggaac
     6361 atatttgatc tacggtgctc caggaaaagg taaaacttca acggctaaat attttccagg
     6421 gaaaacactg atccttgata ttgatcggac ttcaaaagtc cttagaggtg agaaaaatgt
     6481 cgatatcgta tatatcgaca acgaagatac ctggaacgac tggggcaaca cgttggctga
     6541 tttaactacc aattatgtag gtgtttatga caacgtggtt gtcgataatg tcagcgaatt
     6601 ggaacggtgt attctgtcca gtttaggcgc agaaggaaaa aacaacggcg ttccttcgca
     6661 aggcgattat caatatatgc aatttagaat ggtgaacagc ctacgctata tgaaaaattt
     6721 agacagcaac cttatctgga ccgcatggga agaaatcgat ttgtggacgg attcggatgg
     6781 atcttcttac aacatcgctt tgccgcagat caatcgaaag attcgaaaca atattttggg
     6841 attatgtgac gttgttggtc gattgatggt aaaggaagac gatgaacgcg gatttatttt
     6901 acaagccaca gattcaacct atgccaaaaa tcaattggat aatagatctg gctgtaagca
     6961 aagtgagctg attttagatg gaattgtacg actaccagaa tgatttagtc gatcgtgcaa
     7021 gacaagccta tgttgatggt tacaaggcac catgtatcgt atcaccgtgc ggttctggaa
     7081 aatcagtcat ggttgcagag atcgctaaac gaaccacacg aaaaggcaac agagtgttgt
     7141 tcttggtcca tcgtcgtgaa ttgattgatc agatcaaaga tacgtttcaa aagatgggcg
     7201 tagataatca gcttgtaaat ttcggcatgg ttcaaacagt cgtacgacat cttaaaacca
     7261 tcaagaaacc acaactgatc atcactgacg aaaaccatca cgggttagca gcgtcctaca
     7321 gaaaaatcta tgagtattat tcggacgttc cgcgattagg ttttaccgcg acacccatcc
     7381 ggttaaacgg tagtgggttg ggggatgtga acgacttgct aattgaagga gtatcggcta
     7441 aatggttgat tgagaatcat agattagctc cttatgaata ttatgcacct aaattgatcg
     7501 atacagccga attaaagaaa gcttcgactg gagattttac aaagaaatca atggataagg
     7561 cagtgaagaa tactatttat ggcgacgtgt tgaaacatta taaaacgtta gcagaaggtg
     7621 aacaggcaat agcatactgt cacagtatcg atgcaagtaa acacactgct gaaatattca
     7681 acgatgctgg ttataaagca acacatatcg atgcaaaaac tccaaaagat gaacgtgcag
     7741 atattatcga agcgttcaga aatcatgaaa ttaaaatcct atgcaatgtt gatttgatcg
     7801 gagaagggtt tgatgttcct gattgttcga ctgtaattat gttgagacct actcaatcgt
     7861 tatcattgta cattcagcaa tcgatgaggg ggatgcgtta ccgaccagat aaaacgtcaa
     7921 tcatcattga ccatgtgggt aatgtgaacc gttttggatt gcctgatatg gatcgtgaat
     7981 ggagtttaga tacaaaaaag aaaacaaaaa ctgatagtga tttatctatc gttcaatgcg
     8041 cgttttgttt tggtgcttac gaacggccaa aaggcgataa tatttgtccg tattgcggag
     8101 agttgcagcc gattgaagaa cgaaagtcag agttagaaat cgatgaatcg gctgaattaa
     8161 tgaaagtcgg agaaacgaaa attactttga attttgagaa caataaatat tacaacatga
     8221 cggaagatga agcagaaagt atagaggact tatatgccat agcaagagct aaaggattta
     8281 aacctggttg ggcttatatg gctgctaaga ggaaggggtg gttgtaggaa tgggcggtat
     8341 ttttggtaaa aaagcagaag atgttcaagg ttcaataata aatggcgtaa caataatagg
     8401 tgatactgga aaaagagata gttcaggcaa tcaaattgta atcgctagaa ataaaaataa
     8461 cgaagttttt gaatcttctc tatctaatat aaaaagaggt gttgttaacg ggacaggcga
     8521 agagagtggc tacgttaatt taaaaaaagc taatgaaaaa taccatgttg aaggaactta
     8581 cgttaaggct ttgtctgtcg gtggaacaaa aggcgttaat tgggataaac gaagaaataa
     8641 atggaaagca cgattatatt tcaaaggaga atatgtctta caaaaggatt tcaacaccaa
     8701 agaagaagca atccaagcac gacttgcagc agaagaaaaa tatttcaaac ctatcttaga
     8761 aaaatatgaa aataaaaagg agaatgatta acatgacagg atttaactta gacttttcaa
     8821 aagcgcaaca agggaacgaa atcaaagacg gtacgtacga agtggtcgta aacaaagctg
     8881 tagaaaatgc aactaaatca ggcgcagaat ttatcgatat cgatttgatc gttcgtaacg
     8941 atgtggatca accgttccaa aataaacaca ttttcgcaaa aatctggaaa gccaaagcta
     9001 ccggcaaata taacgaaggt atgatcatgg caattgcaca agcgctacag ttggaagacg
     9061 gaaaatcata taacgggttt gatgaattgc tggcggactt tgttttgaaa accgcctcag
     9121 tacgcgtgaa aactgaagag tcaaatggat ataaaaacgt caatgtgaag tcttgggata
     9181 aaacgaacac acggggcgta atgaatcacc aattcaaaaa tggtgatgaa ccatccttcg
     9241 gaccggaacg ttcaagagcg acaacggtta gaaatgacaa tcttccattt taattgaggt
     9301 gtaatcaatg tatgaaaaaa ttccaaaaga gcttaaaaat ctcaaacaat ggtgtgttta
     9361 taagctagtt tgggatgaaa agcggcagaa gcacacgaag atcccttata acgccaacaa
     9421 cgggcataaa gccaaatcca atgacgagag tacctggtct gattttcaga ccgctctcgc
     9481 tgctattgat cactacggaa tgagtggact ggggttcttt ttcaaaccac cttatttcgg
     9541 cattgatatc gataatgctg aaggtgaagt tgagcgattt aaatcaggtg atatagaaga
     9601 aaatattatc tatgaattta tcgaaagcat gagatcctat gctgagtatt ctcaatcagg
     9661 tactggcatc cacattattg ctcgcggaga attgcccggc ggaagacgca ggaaaggtga
     9721 cgtagaaatg taccagaacg gccgattctt cgttatgact ggaaatgccg catcaaaata
     9781 tttagaaata actgagccga atccaaagga tatcaaacgc ctatatgatc gctacgttgg
     9841 agatgaaaaa atcatccaat tcaaagaaga aaatccgctg atgaataccg ttgatttacc
     9901 ggttgaagaa attatcaaac gtgctgaatc gtcttcccaa ggggcaagat tcaaagtctt
     9961 tatgaacggc ggttgggaag ctttttattc ctcgcaatcc gaagcggata tggcgttcgc
    10021 caacgacttg gctttttgga ccggtagaga ctttgaaaaa atggatgaga ttttcagagc
    10081 gtctgcaatg atccgtagca aatatgacca aaaaagggga tcggcaactt acggagaatc
    10141 tctactaaat aaggcaattg ctgatacaaa ttccgtctac aaccccaaac ggaaatctga
    10201 ttttaagatt tttatcaaag accaggaaca gccaaaagag gaaaaatact attcgtacga
    10261 cgacaccggc aacgcggatc ggtttactga tatctacgga acgttagtca aatattcgta
    10321 cattgataaa tcttggtatt actacgatgg gaaagtttgg ctacaggaca ataccggcga
    10381 agttcggaaa atgatcgata cgacagtaga catcatggga aaagaaccgt taacgatccc
    10441 tgaaggcgca gacgatgaaa ccaaagaagc tttgatgaaa gcgaaagaaa aacacgtcaa
    10501 gcgttctcgc agcaatgccg gaaaaaacgc tatgatggac gaattgaaac atcgactatc
    10561 cgtattgccg gaagagttcg acaaagataa aacgctgttc aacacacaaa gcggctatct
    10621 gtctttgcat gacggtcttc tacacgagca tgagatagac aaaatgttta cacgtgtttc
    10681 aaatgtcgag tacacagaat cggtcgattg tcctatgtgg gaagaattta ttaatcagat
    10741 ttttgacaat gatcaagaat taatcagata tatccagaaa tgtgtcggat attccctgac
    10801 cggatctacc agagagcaat gtatgttcat cctctacggt catgggtcaa atggaaagtc
    10861 ggtatttttg gaaattattt ctgaattgat gggaaactat gcgatgacta tgcaggcaca
    10921 aacgatcatg gtcaagcaaa gccaatcatc agcaaacagt gatattgcac gattgaaagg
    10981 tgcgcggttg gtcacttcgt cagaaccgaa tgaaggcgtc cgactggacg aaggtctggt
    11041 caaacaattg actggtggtg ataaagtcac ggctcggcac ctttatggaa aagaattcga
    11101 gttcgaacct gaattcaagt tgtggctggc aacgaaccac aagcccatca tccgtgggac
    11161 cgatgacggc atctggcgac ggttaaattt aattccattc actgtccaaa tcccggatca
    11221 caaaaaggat aaaaatctga aatttaaatt gcagacggag ttgcaaggga tccttaaatg
    11281 ggcaatcgat ggttgcctgt tgtggcagag agagggcttg gagaaaccaa agtcggttgt
    11341 tgcagcgagt caggattatc gaaacgaaat ggatcaaata ggtacattta tcgaaacgtg
    11401 ttgcgaaaca ggtccaggac taaaaatatc aggcggagaa ttatacaaag tttatcgtga
    11461 atgggcatca gataatggcg agcatacttt cactaacacg aagtttggca gagagatttc
    11521 aaagaaatat agcaaagaaa aagctggtgg atttatggtt tataaaggaa tcacattaaa
    11581 accaagaaaa tacgataacg ttagagaact ttttaaataa atctggaggg ttctggaggg
    11641 ttgagaacga aaccctccaa cccttagaaa cgttgatata tcagtattta tattatattt
    11701 ttctttcttt ctggagggtt ttaaaaaaat aataaagtat taaagtaaaa aataaaaagt
    11761 aaattatata aaaaagaata ggtttttggg tctgaccctc caaactctcc accaaaatct
    11821 tcctaaatgc tttgatacca acgattaaac agtctggagg gttacattga aatcagaaca
    11881 tcaaatacaa gacgagatca gaattgcctt atcaaaacat ggttgcacga tttttcgatc
    11941 caatgcggga caagtgacaa cgatcgaagg gcggcaattt tacgggatgc caaaagggtt
    12001 cccagatcta tgtggacatc gtaattctga tgggaaagca atttatatag aagtaaaaaa
    12061 tgaacgcggt aagttgcgtg aagatcaaaa aaggtttgct gaatttttaa acggtcaacc
    12121 tgtactatac ggtgttgcac gatcagtgga agaagcattg aaaattgtgg aggaatcatc
    12181 atgacaacag aagaagtgat tcaaatgcgt attcgaagcc ttcagcgtga gattgacgat
    12241 ctggaacgga caaaggcagt gatggtcaat gaaacggcga ggaaggcaat cgatttgcac
    12301 atagagaatt taagaaggga aatccatcga ttggaggaat gagcgtggat aagaaagcag
    12361 caatgaaacg aatcatcgaa ctgacacatt ctgagaattg gcaagaagac aaagaaatag
    12421 ttgcagaagt ccaaaagctc ggtaaatcaa tgtggagtga aaaaaccaaa cggagaacac
    12481 cgagaaaaat tgcaatctgg catggtgacc gaattctagt aacaggtact gctgaacagt
    12541 tatctgaaat tactggatta agcaaaaaca ttatctggga tagagctaaa cgcgagaacg
    12601 ttgattcgaa aggtcgtcaa tttaaacatt gggagaagaa ataatggagg aactaatcac
    12661 aaaagtagag cagtgggcaa aagataaggg attggatcaa gctgattcca gcaagcaaat
    12721 gttaaaaacg atcgaagaga ttggggaagt tgccgcttct ctagctagaa aagatgaata
    12781 tggtttaaga gacggaattg gagacgtagt agtaaccttg attattttag ctatgcaaaa
    12841 tgatatggat ttgtacgagt gtctgaacca agcatacaac gaaatcaaag gacgcacagg
    12901 aaaaatggta gatggtgtat tcgtgaagtc gagtgatttg gaggacagcg atgaataaaa
    12961 aagtattaat tgataaacaa gcattgattg atgaattaat gaaaatacct ggtgtgggat
    13021 ctaatagtga cgctttagaa acgattaaac gtttcccatc gtatgaaccg cagaagccag
    13081 ttgtgcctaa gtttgtggcg gaactactcg actattatcg tcagtcaaca gatgttgatt
    13141 tattagcttt attgataact ttccatgatt ggtattatcg caaaactaaa gacggtgagc
    13201 atgaagaagc aattgattgg ctagttgatc atcctgaaat ttatatgcgt gcatggctgt
    13261 ttggctacga gctcgagaaa gagccgttgt attgcgtaat aatcaatggt agatatcttg
    13321 tcaaagtatt cagcaataca aacgttgtca tattagttcc agcagatgaa ttttcacagt
    13381 acgttactca aaaatttcaa ttaactgaaa aacaaatcaa agcaattgat gaaagatact
    13441 ggccgtttgc tgtgccagtg gatgaggtgg tagaaggatg agcgacccat acattactaa
    13501 attagaaaag ttgtccgaag attttgagaa gaataaaaat aaactaacta aagataaact
    13561 tatagaactg gttaggttgg ttatcagagg agctattaca agagaaaagg cgcagcaaaa
    13621 catagaaatt tttgctcgta gtgtccgaga tggattagag aagaaagtta aacaaaatga
    13681 acaacagaca ccgcagaata acaaaactaa gaaaacggga actgaatgta ctaaagacaa
    13741 agtttgaaaa agaatatgga atttcagcag aagaaacata taaagtggca agtcagtgtg
    13801 ttgctgatgc gagcggcgct attcgtaagt ttggaatttc gatattaagt gatgatcgta
    13861 aatgggagga aaaagaatga aactaaaaga cggattttac tccagcagtc atggtatcgg
    13921 cggtttaatg ctagatatgc cgacaaagaa ccctaaaaca cgtgagaaac caaaattcaa
    13981 agtcggtgac atggttcgct gtgaagcaga aggattcatc tatccatttt gtggatatgt
    14041 agaacatctc tataatcact cagcaatcat tcgtattgaa aacacgatgg aatgtgacaa
    14101 gtggttagcg aaaagcaaag agaatttagc agtagcgaga ttggtggata tggaggttat
    14161 ataaacagcg aaaaaatgta cttctaagag aaaaatttat tttttataaa aataggaaag
    14221 tatcattatg aaaaagacca cagagataaa taaacctacc acataaaaaa tgaatatttc
    14281 ttgcgattca atataggtat atttaacaat ttgtacaata aaaaatacta taggtaaaaa
    14341 agataacatg aggagcttat ttgttctaga gtgtttgaca aagtaacaaa atgcgacgat
    14401 accaatacaa acgggaatcc aaaataacag catgctccac atagtcactc aaccatctcc
    14461 tatacgcttt attatcaata gataatatca tttttcgtaa taaagataaa aaaataagta
    14521 aggcttctca ttaaaatata aaaaagacag ccgaccactg actgcctata taagagtatt
    14581 gaaataaaaa gctgctgata taataaattc cacaagttta ttatatcaca taaaggagcg
    14641 gtttgacttg atgcaattgt tacgagaggt agatttcaaa cagacaagat gtaatgcgag
    14701 agatgtgctg aagaactttc ggcgtttgga gcggatggca ggtcgctctt tgatagatat
    14761 taagtcgccg attattacgg atatgccgaa ggcgccgaag cacggcaata aggcagaaga
    14821 cgcaatcatt cagatgatgg atatagaagc ggagagagac gcgattctag cagccttgat
    14881 ggctcttagt ctgattagtc gtcagatact ctactatagc ttttgtgtgc cagatagctt
    14941 ctcaaactac agaattagcc gtgaagtggg ttattcagaa agaagtatac aacggatgaa
    15001 gtcggaagct ctaatagagt ttgcagaagc atataaacac ggaagaataa ttgcttataa
    15061 ataatttggc ggctttttgg cggaatgatg gcggttttta gctatttacc agtgatatta
    15121 tggtagtgtc gaaagattag tgataggtct aagacaaaat aataataaaa ggaacatcgt
    15181 tttattattg tttcacaatt aagcttcgat agacagcaac ggaaatatta agaataagga
    15241 tgtgaatttt aactccttct aaattgttct tattatctat catccgttgc tgtctattgt
    15301 ttttttaatt attcacacga taactaaagg tgagcgaaga gaaatgattc cattaataat
    15361 ttcaattttt gcgctttgtc ttaatgtcta tatgattgga tttaaaaatg ggcaaaataa
    15421 aagatagtag taactaagaa agaaattttt atattgtcac tgtggcggaa agggtagacg
    15481 ctaagcatgt gtgctaggtc aatgcttcgg caactatgca atgttcgatt cattgccagt
    15541 gactttggca accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg
    15601 ttttgtgcat cgtgtaggtt gctattacat attagatcac tcgttgagtg gtctttttat
    15661 tttacataaa ggaggtaaca acgatgtata gaccacaata cttagaacag aagtatgaag
    15721 taatcactgt gcaaaacggt aacggtgaga tagtacgaaa gtatagaaga ccaataaaga
    15781 gcgatacata taaacgaaag gaaaacaatg aagttatttc ttttcgcaga aggagaaaag
    15841 ccaaatgaga aactactggt atgtatcgct aactaatgaa tatcctcgaa ccattgatga
    15901 ttgttcagtg cgtgttgtgc gttctgtaca aatcaaaggg aagtactcta ttgtcgaaat
    15961 gctaagagaa gctacaccaa acgaagtgga taaatgcaag ctgatatatt gcggtcatgg
    16021 ctattggaaa gacgagtata tccaacaaaa cattgagagg tggatagata gatgagttac
    16081 ctcgaacatt tgaaacgttg ctacatgcat tctaagaata aacttcctga cagctacact
    16141 gtagatgatg ttgctataca tgtattgaag actgagagcc atagcagtcc tgatggtagc
    16201 agtaaggagc agacgctcgc gtggttcaag ttctttaagt ggattaagga ggaagagtaa
    16261 tgcacatgca cataggagaa aagatatata aattgagacg gcttaataac gaaacaatga
    16321 acgacttagt taaacaaact ggagtgtcaa aatcatggtt atctgatatt gagtctggaa
    16381 aaaagcaacg cgttgatttt aataagatat gtgcaatagc caaacattat aaagttagct
    16441 tagaatactt tcgggatgat tatttggata gtgatgaaca cgaggatatt attcctttga
    16501 caggcgaaac aagagttaaa tcagtgaaat cgcttgaaca aagcctaaag tatctgaata
    16561 acaaacaaga acctaacctt ctagagatca aactaaaaga tactgactca gtaccagagg
    16621 tttggtacaa gggtgagagg ttggatgaat tgcctaaagg attagtagat gtctcgtatc
    16681 attggaagac tgatgatttt actaatgatg atagaggagc gaacgacatc acgattcaat
    16741 acttttctag ctttaatgat aagtatccag ataagaaaac aatcggacac aagagagata
    16801 tgtaaatgaa agaagttaga cctagagacg aaatagacaa actatacaag accaaacgat
    16861 ggcgagacct aaggcaagta gtaatagcta gggacttcgg catgtgccaa gagtgcaagc
    16921 gtcgagggcg gaacacaagg ggcacgatca tccatcacat agtcgaggcg agggaagacc
    16981 tgtcactgtt ctggtccgta gataaccttg aatgtatctg tgtagcttgt cacaacagag
    17041 agcatccaga gagatcaggc gggaagaaga aaccaaaacc taaatcacat atcgttaaaa
    17101 tgtattcaac tcctgaaaga taagtttgca gcgaaatgaa ggtagccccc ctactctaaa
    17161 agattaaaga gtaaggcttg agaagaacgg tgctgtcctt ccttcgtaaa aagaccgctt
    17221 ttcaagtttt ttggagaaaa aggaaaaagc cgaccaattt aagccggctt tggacgaagc
    17281 tatttcttag tccatttgtt tcctttttga gaagtagggg gtaatcggtc gcctggatca
    17341 atttttactt cttgtccgcc ttggacattt ccaccacgag gacccacttc tttgtaggtt
    17401 ccttttggtt tgttgtcttc gccgggttta tagagttctc ccataggaat ccctccttaa
    17461 caaatttcgg cacagcactg ccgataacct aattataagg attgtgataa cgatttcaat
    17521 ctatcttttg aaagaaggtg atattatgcc gcaaccagcg aagagtgcaa aattacaatt
    17581 attaaacgga aacccaaata agaagaatac cgaagaactc cgcaagcgag cggccgcaga
    17641 agacaaatta aaaatggcta ctgacaaaat caaaccgccg ttatggctag attcgctagg
    17701 aaaggatacc tttgagttta tcgccgatga attgctgtct gtggatttaa tcagtaatcc
    17761 ggacgtccat acaatggctc tatactccaa ttggtattcg caatacgttt ctttagaaaa
    17821 acagcttcga aaactacaac gagagtacaa gttgaactat gcgcttgcga aaaaggaggc
    17881 agaggcgaga ggtgagccgt ttaatgaacc taatgaatta attggtaatc cgctctctcg
    17941 gcagatggat acagcgtctc ggaatctccg ttcttttggc gctgatttag gactatcacc
    18001 agcagccaga gctaagttag ctattaagat ggctgatgat ggtggtgatg acgatgacga
    18061 cttctaatat tttggatatg tcttacacag aacgtgtgga ctattggcaa agctatcttg
    18121 aggagcaagc ttcttggggt ggctttttaa aatgtccata tccggaattg ttaactactt
    18181 ggtatgcgga acgattaatc gatggaagca taccagccag caaagaaaat attcaagctg
    18241 ctaaacggca tatgcgtgat ttgcagcgcc aaggaacaga tgattttcct tggatctttg
    18301 acgaagaaaa aggtcaccgg cctattagat atatcgaaaa aaaatgtaaa ccaactgaag
    18361 gcgactttgg ttcgtttgtt ttgcaacctt ggcagcattt cataattgga tccatgtacg
    18421 gatgggtaca tcgtgataca ggagagcgtc gcttccgcga ggctcttatt tttgttggac
    18481 gtaaaaacgg taagttttgc cgtttaaaaa tcgggcaaaa tcggtgaaaa cctttatttt
    18541 tggctttctt ttagtaataa ttcgatagct ttatcaagca atttagacat aggaaccata
    18601 gtttcttccg acattttttt taatccttcg tataaatctt tatcaattgc gttagaaatt
    18661 ctaattctgt tttttagtcc gtatttgtcc atgtgtgaca ctccttttta ttttactata
    18721 acatccgatt ccaccgcttg caactaccat caattgatgg tagtatataa aagaggtgag
    18781 tagattgaaa aaatacatta tttacaaaca cttaaataaa ataaatggca aaatttatat
    18841 cggtgtcaca aatgatattg gcagaaggtg gaggagcggt gggattgaat ataaaccacc
    18901 aaagaacgaa acccaacacc acagaagttt ttggaatgca atacaaaaat acgggtggga
    18961 aaattttgat catttgatta ttgaagaaga cttaacgatg aaagaagctt ttgagaaaga
    19021 aaagttttat attgaacttt atgactcaac taataagaaa aaaggttata acatcgcaaa
    19081 aggcggaaat ggcggaataa tatataaagt tcaccccaaa ggaatgctag ggaaaaaaca
    19141 atctaaagaa ttttcttcaa atcacagtaa atgggcaaag aaccataaga ataattgtat
    19201 gacaaacggt gatgttgtat ggggtgttac tcacgaacac ccaaaaggca tgttgggaaa
    19261 gcaccacagc aaagaatcaa ttatgaaaaa gaaagcatat agtggtgaaa atgctgtaac
    19321 ctcaaaacca attgtcgcaa ttgaacggga tggaactaaa agagaatttc acagcgctaa
    19381 gctttgcatg gcttactatt cgattagtac atgtgttttt taccgacttt taaaagatgg
    19441 cgccccttat gtgataaatc ctaaagcgaa ctacagaaat aaagaaaaaa tattagctat
    19501 cgaaggaatt atgttcaaac ataaagaaga taccgaggta agttagtaga ttgcgaaagg
    19561 ttactaacta ccgtaacgcg taggagttga ataaatataa tgctcccaag agtgtccgac
    19621 agcagataat ttgctgaaaa tgtacgctaa actgggccag aactgactga ccgatgaaaa
    19681 tgagggtgac ctccagagtg cgagataaaa agctcgcaga taataacaat cgaaaacatc
    19741 actaatttca ggcctttcca catacatggt cgcttatgat gatgaacaag gcgccaacgt
    19801 ttacgtattg gcaaatgctc gtgatcaagc aagcttgttg tttgataagg ccgcagaaat
    19861 ggtcaaacaa tcgccggcgc tctttaagaa atttggtaag cctaaacgat caagtattaa
    19921 ttatgctccc gccttttcta aaatggaacc acgcgcctca gatagccgga aattggatgg
    19981 gctaaacact cactttggta tttttgacga gatccacgag tttacgaatt acaatctgat
    20041 caacgttatc aagaaatcaa gaggaaccag aaaacagcct ctgatagttt atatcacaac
    20101 tgctggatat gtattagatg gtccgttgat gtcttatttt gagcaaggtg tggactgttt
    20161 ggaacatttg gaagatgaca tcgatgaacg gactttctat tatctggcaa aacttgacag
    20221 tgcggaagag gctgatgacc caagattatg gatcaaagcc aatccgaata tttgtctaat
    20281 gaattttgtt ggcatgctag atgactatgt taaggataaa aaagatccaa aagaatatgc
    20341 tgactggatt accaagcaat ttaacttgtt ttccgatatc gatgagctgt catttgtcga
    20401 tatgcctacc attaaacgaa acaataaaac catcgatatt gaaacgctca aaggtaagaa
    20461 gtgtgtcggt ggttttgact tgtccgaaac ggaagacttt accgcagccg ttttagaatt
    20521 tccgcttgaa acaggcgagg tattcatttt gcaacacaca tggatcccac aagctagatt
    20581 tgatcgagat aacaatcaag agcgtatcaa agcgtgggag aaggtgggag atctaacgat
    20641 tattcctggt gattacgtca attatgaata cgtcttaaat tggtttgtag aaaattcgaa
    20701 aatctatgac attgtaaaaa tcaattatga caaggccaag gcgctacgat taaataaaga
    20761 actagaaaat gcaggatttg aaaccaacga gattcggcaa gggtttctat cattaggtgg
    20821 gccaatgcaa aacttcaagg aaatgctatt ggacggtaag gtgattttca acaattccaa
    20881 gctttaccga tggtatctat ccaacgtcaa gctggtgatg gatcgcaact caaactggat
    20941 gccgtctaag cagtccaaga gtagaaaaat agatggtttt gcagcaagtt tgaacagcca
    21001 cgccgaagtg ttgaatatgt tggttaatcc tgtcggaacc gggaaagtaa cctattactc
    21061 gatttccgat ttaatgaata tgtaagaaag gtgtggagga atgagtattt tagatcgttt
    21121 gcgttctttt ggccgagcga agccgaaagc gagcaaacaa gagtattttt tgaatgaccc
    21181 gggattgata ccgtatttag tcggaaaaga tgaaatatca gaagggattt tttccgtaat
    21241 tagccgtgta tcgaacgttt ttgcgtctct ccctctcaaa atgatagatg tggagtttgg
    21301 ccaaccggac gactgtcctg catacaactt gttgagcgaa ggccctcgat attttacaaa
    21361 gtttgatttt ttccgggacg tggaagtttt gagaaactac caagggaatg cgtatgtgca
    21421 gattttccga aatatcaatg gagaagtagc agatatggcg ttagtaaaac ctggtgcttg
    21481 ccatccagtg attgatatgg atagcgggga gctttactac caagtaactg cgactgacaa
    21541 aggcagttac aagcaagtta tctatgtaca ttacatggaa atgcttcact ttaaacaacc
    21601 gaggtttggc ggcttggaag gtacagaccc cacaaaagta ttaacgaata ccctcggata
    21661 tgatcgagaa gtccgaaaaa tctctttaag tcagcttaaa ggaagtaatg aagggctaaa
    21721 agttaagttt gctagcaata tggatgaaga agctaaaaaa gctacagtta aaaacattgc
    21781 tgatttttat cgacaaaacg gtggactact tgtggaagaa aacggtgtag aaatcgaacg
    21841 tttacaacga gagctggtag acagcaagct tttagatact gataaagtat ctcgctccag
    21901 aatcgctatg gtctacaatg tgccggagca tttcatcggg aataaccagt cgagttactc
    21961 ctctcaggaa cagctcaata tggaattttt gacgtacaat ctagtaccga ccgttaatca
    22021 atatgaagcg gaactaaata agaaaacact atcgagaacc gaaaaagcaa aaggttatcg
    22081 atataagttt aatgtcgcaa gtttgctaag agctgacact caggcaaggg gacaattcta
    22141 tcaaatcatg cgacgaggcg gagcatactc agcgaatgat gtccgaagat ttgaggactt
    22201 gcaaccgata aataaaaccg gtatggatga ttaccatatt tccggagacc tatatccaat
    22261 cgatatggat ccaacattaa gaaaaacaac ctcgtctaaa agcgtagccg aaaacggtta
    22321 ggttttttta gtttgcaccg aagggaggtg gaaaaatgaa aaaagtgacg ttaagcggcg
    22381 atgtcgtgga taacgatacc gcgtggcttt atgactggtt tgggatcaat tgtatctcac
    22441 cagggaaaat ttctgccgct cttacagaag cagcggggga tgaagtagaa cttgatatct
    22501 catcgaacgg tggggatgtc ctagcggcaa gcgaaatata taccgctatc cgtgcctatc
    22561 cgggaaaggt atccggaaat gttgtgagta ttgcagcaag cgctgcaagt gtaatcgctt
    22621 gcgcttgcga accgcttaga atctcaccta cggcacacat catgattcat aacgcatggg
    22681 tgaccactag tggcaacgct gaggaattaa aagccaatgc agaaatgtta agcagtgtgg
    22741 atgagtctat tgttaatgct tacgagatca aaacaggact cgatcggaaa aaacttgctg
    22801 atttaatggc gaaagatact tggttaaatg ctcaaacagc agtagcagaa gggtttgcag
    22861 atgaaattat gtttgcagaa gcaccagtaa cggtactcaa tgcctctcaa ccggttattc
    22921 caaaaaacgc agtaactaag ttgaaaaatt taatactcaa agcggaagca ccgcaagaag
    22981 aaacactctt acagaaaaaa ctaaaagcct taaatggagg gaaaaacgaa tgaatttaga
    23041 acaattaaaa aatgcgtggg tcgaggcggg aagtaaagtc tctgacttaa atgcacaact
    23101 caatgcagca ttggttgacg atgaaaaaac agaagaagat gtagtaagtt tgcaagcaca
    23161 agtaaaagca gcacgggcta aacgggacgg attgaaagag caagtggcaa atatggaagc
    23221 cgaacaagtc ttaaacgtca aaaaagaacc attagataaa aaagatgaaa acttgaaaaa
    23281 caagtttatc aaagacttta aagcgatggt caatggtgat cctgctatta tggctacttt
    23341 gacatctaat acggatgaat ctggtaatgc tatcggattg actattcctg tagatgtgca
    23401 aacgactatt catactttgg ttcgtcggtt tgactctttg caagaatacg taaacgttga
    23461 aaaagtgacc actaccagcg gttctcgcgt ttatgaaaaa tggtctgata ttacaccgtt
    23521 gactgctttg gatactgaag acggtgaaat cccagcaaat gatgatcctg cactttactt
    23581 gatcaaatac ttgatcaaac gctatgcagg tatttccaca gtaactaaca gcttgctaaa
    23641 agatactgcc gaaaacattt tggcatggtt gtctaaatgg atcgcgaaaa aagtagttgt
    23701 tactcgcaat acaaaaatct tggcagctat tgatggaatc aaagcggcac aaaagaaaga
    23761 tgttacagat gttgatggaa ttaaagatat cgtaaacgtc caacttgatc cagctatcga
    23821 agctacatct atgtttatta caaaccaaga tggcttcaat gttttagata aagtgaaacg
    23881 tgctgatgga tcttacttgt tacaaaaaga cgtagcttct gcaactggat ataccttctt
    23941 aggtaaacca atcaagaaaa ttgcttctcg tttcttgcca aacaaaggta cgcaagctac
    24001 tcctaaatat ccactgtaca ttggtgatct gaaagaagcc gttacattgt atgatcgcga
    24061 aaacatgagc ttgctgacaa cgaatattgg tggtggagct tttgaaacag acaccactaa
    24121 agtacgcgtc atcgatcgct tcgatgtgca actagttgat gatgaagcgg ttgttttggc
    24181 tacttttaca actattgcga acgagacacc ggcgaaagtt taaggagctg attccttatg
    24241 attcttgatc ctaaaatgga tttaggcgaa atcaaaaacg cactaaagat tgataccgat
    24301 gatgacgatg tggaagtaag ccgtgcggca caagctgcaa ttgcatacat tagaggggct
    24361 atcgggaatg ataagccctc tttttataag caagaaaatg aaacggttga tctgattaat
    24421 ttagctatcc tgcaattagc agatcattat tacaacgctc gatcagcaac cgtgagtggg
    24481 aacttgcgag agtacgattt aggttttaca agcctaatct tgcaactcaa agcaagttat
    24541 ttgctttttg tggaggagga gtagcgtatg ccccttatcc aaacaggaaa tttaaatcaa
    24601 cgcatcaagt ttgtccgaga tacgacggtt aaggatgagg acgggcaagt tgtcccgact
    24661 tctacaacca ttcttacttg ctgggcaagt gtgcagacac aacgcctgaa cgatattaag
    24721 acgtcgattg gtacggcttt ggaaggaaca ctgacgttca ttatccgcta ccaacaaaaa
    24781 tcagagctga ccaatgatat gaaagtgcgt tggaatggaa aaacgtttga aatcattacg
    24841 attacgaaag gcgagtttgc gaaggacttc acgacaatca ttgcaaaaga ggtttcaaaa
    24901 tgagtgtaga agtcgatgca accgaagtgt acaaagcgct tagggaagta aaagcaaacg
    24961 ttcaacgagt ggaaagccca gcacttagaa aagctgggga gtacgctcaa gaagagttac
    25021 gacaaaacac accttactgg gatggaacga agtcaaacgg taaacgtggt tcgtatatgc
    25081 aagaacatgc taagaaccat gtggttacaa gctcgataaa aaacggattg gtagaagtcg
    25141 gctatgacaa agatgtttct tggcggatgc actttatcga gttcggaaca atcaaacaac
    25201 gtccaaaagg tttcgtacaa aaaacacaaa agcaaatcga aaaacaagta acacaaatca
    25261 ttgctgacga agtaaaaagg aggctaggac tttgaaaacg gcagtatcac aagtctattc
    25321 aattctgaat agcaatgaaa aaacaaagaa cattgatttt tacaccaata gtgttccgga
    25381 atcagctcaa acagtaccta gccttccagt tggcagaatt acagagatat ccggcaacta
    25441 tgaagatttt gcaagcaaca atcctttgac cattcaattt aacgtacagg tagatgtatg
    25501 ggtgtcaacc atgaaagagg ttgatgcctt ttattttgcc cttgatgagg ttatgagggg
    25561 gaatggttgg caatgcgcat acacggaaca aacagatgac gaggacttgg aaggtgcaaa
    25621 gcggattatc aaacgatatg tagcaaatat ttcactaaat taaaaggaga gaaaatagat
    25681 ggcaacagta ggattcgaga gcgtcatttt tggcgtaaaa acaggtgcag gcggcactct
    25741 aaaagaatta gtagcagata agtcgaaagg cggagcgatc gaagctaaaa ttactggatt
    25801 aggcgcaact tctaacacaa catacgcttc aaacgtaccg ttcttcattg caagtaaagg
    25861 ggtttcgtcg ccaaaagtta cgcttgacgt ggcagactta atggataacg gcatttacag
    25921 cgaaatcatt ggtgctaaaa ccgtggatgg tgcaaatgta attggttcag aaactgaagc
    25981 gccttacgtg tcggtagtca tggttacagc gaacaaagaa ggaaaacgct tgttcatggg
    26041 attgacaaaa ggaaaattca gtcatccaga tatcgacatg aaaacagctg aagacaaagg
    26101 ggtagaattg caaaccgatt ccatcgaagg ggaattcatt tctgatgaac gtggctatgt
    26161 atacttaaca gccgtagaat cagaaggaat gaccttacaa aaattcaagg acttggtaaa
    26221 taacaaagag ggggagtagt taaccctgca tctacaccaa cgacagatac agggacacca
    26281 aaagaaccag aaccaaaaat tgatacacaa ggttagccat ttttggctag ccttattttt
    26341 tgtaaaaaca aggaggaaaa caaatgattg aattgcaatt gaaacttgac ggaaagaaaa
    26401 aaacattcaa acaacaagat atttccgcac gtgcaatgcg tgagtgtatc aaattttacg
    26461 agaaagcgga aaaagcagac ctaactgatt tagaagcgat tgattcaatg attgcaatta
    26521 cagcagatat tttccaagat ccagcagtta catttgatgc tattttagac ggtttgactg
    26581 cgagcgagtt agtaccggca ttagaaagtg tttttgaaca aataaatgaa ctgggaaaca
    26641 atgaaaaaaa gcagacggcg agcaaaaaga aataagtttt tctgaagcta ggaaagcaat
    26701 ggatcaaatc tacaaagatt taatcgaagc aggttggacg atgagagatg tggacgaagc
    26761 cgactatcat tatttgttac acctttttgg agaagtggag agtggcgaag aatatgtaga
    26821 tggtgctgat ttcatcaaac aatttttatc ggctgaagac ttagtaaaac ttgaggaagg
    26881 aggtaaataa tggcaggaaa aggacaaccg gcaggaaata tcaagctagg gattagttta
    26941 gatagcacta gttttggtaa cacgctggac gaaatcaatg cgaaagtcaa acaagctgag
    27001 tcgaatatgc gtgccaatct aaaggcttat gattcagcag gacgttcata cgaagcactt
    27061 agtcaaaaga cgaaagactt gtctacggtt atggaagggc aaaacgccaa agtaagagaa
    27121 ttaacaaagc gccgtgatga agcgattagc aagtatggcg aggaatcgaa acaagttgct
    27181 aaccttaaca cacagataaa caatgctacc gcaaaatata atgcttacag tcgccagttg
    27241 aacgacacaa aaaaagaatt ggtgtattcc aaaacagccg tcaatgattt atctaatgaa
    27301 atcaaagaaa atgaacgaca aatgaacgcc gaagtaaaag cgttgaaagc cgctggtgat
    27361 gaatctggtg cttttgaagc aaaacaaaaa gggctagcca aacaaacgga attatccgag
    27421 aaagctatcg aagaacagcg caaagttgtg aaactgatgg ctgatgagtt tggcgattca
    27481 gcaaatgaaa ccgaagatgc aaaaagggca ttagaaaagt tagaacgaca aagccaaata
    27541 tctagcaggc aattagaagc actcaaaagc tccagcgatc aatcaggaaa agaaatagaa
    27601 gattttggcg acaagtccac aaggtcagct aggaaactgg acggactaaa agacaaatta
    27661 agctcgctaa aaagcgcatt ttcgtttggt gcagttgctg gattagcgca taacgctatt
    27721 agcagtgtag taagtggcgt gcaaggtttg gttggcgaag cagtaaacgc atcggattca
    27781 ttgatgaagt tttccaaaac catggagttt gctaactttg ggaagtcaca gatagaaagc
    27841 tcgaaaaaag aaatgaaaga ctacgccgat aagacggttt atggtttaga agaaattctg
    27901 aacacaaccg cacaattggc atctaatggg attcctaact atacagaact aaccaaggcg
    27961 gcaggtaact tgaatgccgt tgcaggcggt tctagtgata cattcaaatc cgttgccatg
    28021 atgctaacgc agacggcagg agctgggaaa ctaacaactg aaaactggaa tcaattagca
    28081 gatgcgatac cgggtgcttc aggattgtta caagacgcta tgttgaaaaa cggagcttat
    28141 acaggaaact tccgtgatgc aatggcgcaa ggtcaaatca cttccgacga gttcaaccaa
    28201 gcaattgtac agttaggtat gaatgacgga gcagttaagg cagccacttc tacagacaca
    28261 ttgagcggtt cttgggagca gatgaaatcc actgtaataa atgggctaca aagtattata
    28321 gaaaaaatag gcgttgaaaa tatcactggt ttcatcaaca gagtaacaaa agggattgaa
    28381 aattctattc ctaaaattac tcaatttata ggttggttga gagatattgg aacgtggatc
    28441 gttgaaaata gagagccact aacatggatt gtcggaatca taggcggaat tacattagca
    28501 gtaaaagcat tgaacgtagc aagtatgttg ctggcaatta ctggcggaac attggcagcc
    28561 ccttttgtgg cgattggtgt agcattaggc gcactagcag gtgctttggt gatagcttat
    28621 acaaaatctg aaacatttag aaatatagtc aatgcggctt ttacagctgt gaaaaacgta
    28681 gttatgagcg ttatcaataa tttggtggaa tactacaaaa tgttgtggag cgtgttgcag
    28741 tggctttggg aaaaaataaa agaatgggct tcatggattg gcaataaatt cattgaaatg
    28801 aagaacagcg ttgtgaatac ggttcagaac ctatggaatg gcgtgaaaaa cttcttcagc
    28861 aatggcgttg gagatacgtg gaataaggta gtcggttggg taaaaaacat tttcaacaaa
    28921 gcaactgaat tgaagaacaa cgtttctgat gtaatcggta acctgtggaa tggtatcaaa
    28981 gacacattcc gtagaggtat cgatacggta ttcaattggt tttcagaact accaacgaag
    29041 atgaagaatg ccattattgg cggtaaaaac gccattgttg atgcgttcaa aagtattttc
    29101 aacgcagcac ttaaagcgat aggtaaacca gttaacgcaa tcatccatgg agcttcatgg
    29161 gtactagaaa aattgggcgc agaacctcta caagaatggg atgtaccaca atacgctaca
    29221 ggtacaccag caggtgggca cccaatcaat ggtccaatga tggttaatga tggacgtgga
    29281 gcagaaacag ttatcacacc agatggtaga gccttcattc ctaaaggacg caatgttgtt
    29341 ctaaacgcac caaaaggaac gcatgtcttg acagcagaag agaccgcgca acttcaaggt
    29401 tctaaagctc ctaagtatcg ttacaaaaaa ggtactaact tctttggtaa catgtgggat
    29461 agtgtgaaaa aggttgctgg taatgtaggt cacacaatta aaaacgtagt aggtgacgtg
    29521 tgggacttta tttcagaccc gggagcgtta gctagaaaag tacttggggg gttagatgta
    29581 ttaggtggat tgacaaagta tccattagaa gtaggtaaag gcatcctatc taaagcaaca
    29641 agtgcactga ctgaaaaaat cactgggttg ttctcatctg gtaacttaga tacctccgta
    29701 ggaacaaatg gcgtctataa atatttggct gatgttgcta agtctgtgat gaagaaattc
    29761 ccaggatttg tggcaactag tgggtataga ccaggtgacc cttattcaca tggtaaacgt
    29821 aatgccattg atattgcatt accaggcgtc acaggaggct cacctagata cacagaagca
    29881 gcaaactacg cttttgacaa attcgcttcc aaaattggtt acgtaatcac taatgggaaa
    29941 gttcgtgacc gttcaggaca atcaggtcaa ccagcaactg gtgcatggga gccatggccc
    30001 gatggagatc actatgatca tgtgcattta aacggtgtga aagacccaca aaacactcaa
    30061 atttcaggag atagcgtggg aggcagtggg gtagaaagat ggcgcaatgt agcaattaga
    30121 gcgttgaaaa tgaccggtca atacagtact gcaaacttaa atgcattact aaatcaaatg
    30181 cgtacagagt caaatggtaa tcctaatgca gttaacaatt gggatattaa cgccaaaaat
    30241 ggaacaccat caaaagggtt gctccaagtg attgacccaa cattcagaca gtatgcaatg
    30301 ccaggattca acagcaatat ttttgaccca ctatctaaca tcttagcttc aatcagatac
    30361 gcactatcaa gatatggctc actaacaaat gcctatcgtg gagttggtta cgcaaacggt
    30421 ggaattgtaa accaacatca aattgcggaa atcgcagaag gaaacaagcc agaaattatt
    30481 attccgttag ataaggctaa acgatcaaga gcgatgcaat tattgtctat cgcaatggac
    30541 aaactaggag tttctcctaa aacttatgga aatgcgacaa ctgtttccag tgattacgaa
    30601 atcctagaag ccattgaaaa acaggcggca acaacgaatc agctgttatc gttattgctg
    30661 gcatttttca aaggaaatag ccgaactgat agagatttag ctttagatat tcagaagatc
    30721 ttggttagga ggatgtagat gcgaactgta ttactgagaa atagaataaa cgaagaaatt
    30781 gatttgtcca cagaagacta ttttgctact gggttgagta atatggggtt tgaagtaaaa
    30841 aaagaacatg tgggacagtg ggggaatttc agagaaagca gtgagagcgt tgaaatatct
    30901 gaatttcagt catctgtaat catttctgtg catgggtttc gagaaaaaga actgtacaat
    30961 tcacttgtgc agttcctatc ggaaggtccg tttgaactgg aatttgcttt tgacggcgaa
    31021 acaatggtaa gaagatgcag tctaaaatct ttaagtaaga ctgaaatcga tccgaaaaca
    31081 tcgttactaa cagacacttt agaactatat ttcacatcaa actggtattc cgtaaaacgc
    31141 gaaaaattaa ttcagcgacc gaatgtagtg aaaacacgtg gtaaggtttt tccgtataaa
    31201 agatcttata tctacactca aaacttgtgg gagaaaaaag gtgtattcaa attcaataat
    31261 aattctgtat acctaacgaa tagcaaggaa cggatgtccc cgctaaaaat tcgtgtgatt
    31321 gggaagtgtt caaatccgta ttgggaagta atccaaaact cacaaatcat cgcaacggat
    31381 ggatacttca tagatatgac tgaaacacag actctagaag tatcaagcct ttttgaagat
    31441 acaacggcga ttttaaaaga tattgcaggg gtagaatctt ctgtctacca acaacaggat
    31501 tatacaaaaa ctaattttgt tcaggcccca actggagaat tcagcattgt gtttcatgtt
    31561 gggggggcag acgtagagat tgagttatac gaggagcgtg atctgttttg attttagcag
    31621 tcacgctttt tcatcgtgat ttaatgcttt ataacgaaca tttattctct aacagttttg
    31681 aatttggtgt ggacgagatc aacgaagagg ctagtagctt tacgatggat aagtacgttc
    31741 ctgtaaaaac aggcgatttt ctactagcaa aatatattcc tagtggaaaa tttgcatatt
    31801 ttggtgtaat tacgtcgcag gaagacgaaa aaatcagttg taaaagctta cttagtttag
    31861 ctgatagtga gataccgact gcacgtgtgt caggagataa ctacgaagag catatccggc
    31921 ggttgattga atactatttg ctgaatgatc caacgaaaca actaaaagat attttagacg
    31981 tcaaagcaga aagtgcgacc tctcattcgt atcaagctac cgatacgaat aaacacaagt
    32041 taagtgcgta tattctcaat ggtttcaaaa aatacaacgt aaaatggtat ttcaagggaa
    32101 ttcaaaacag aaaaatttat acaggcatac gtgctgtaaa tgaatcaatt tacattaaag
    32161 acaattcttc tgaatttagc gattgggatg tgtttgttca ggcgccgggc gctggaaacg
    32221 agaacaagct attaatcgtt gataaagcaa tgaaagatat agagaaaccg ataatactgt
    32281 caacatggta tttggacgaa gaaaacaatt tgacacaaga tggatcgaaa gaaaatatca
    32341 cgaaaccaac tgtcaatttg gttaatatct acgatcaaac cgcagaagat aaagcatcct
    32401 acgaagatgt ggcaaaatca gagttgaaag gcaatacgta ttcgcatgaa atcaaagtga
    32461 acgttgtaag aaatgcaaaa aatttgaatg tcgaaacgat tgaaacaggg atgtttgcca
    32521 cgatttctta taaaggaaag atatacaagt cggttttgac agcttggcga atatcaagtg
    32581 ataaggagtt tgtggaattg actttcggaa acataagaag tcgttttatg gattattttg
    32641 aagataatgg gggataaaaa atggttagca atgtggatgg atatcaattt gaaaacgtga
    32701 aagtaagcgc agaaaatgat gctagacttt atcacgtttt atataatcgg aaaaatcagg
    32761 ttattgatgg ttacgatcag tctatgaatt tatcttcaag cggattaaca gtaaaagttg
    32821 ctgcaggagc agcgattatt caaggtcgta tggtcgttgt tcgacaagaa gaaagtataa
    32881 cagttccagc aaactcaagt ggttatatag cattaacagt ggatttgaca caagaagtta
    32941 tacctggatc tattcttcca gaatcggaag aatatgaatg gactaataat caagtcaagc
    33001 tagaatttat aacaaaagtt ataaaaggta atttaaacaa tggtgataag gtctataatt
    33061 taccactatg ctcagttaat tctactggat caactgtttc aatctcaaag atatcggata
    33121 gttacgagct gactctctct aaaggagaaa ttttgtggag ggggactgcg ttaatgcatg
    33181 atactcaaac tgtccaacct tcaaaaaaaa tttggcagac agtttcaggt tttttgttag
    33241 tatggctccc atacgaaaac gggcaagcaa ttgaggatag atatgtaact acgccttttt
    33301 ataaggagcg tgtaactttt actaatgttt taggagaaat tgtttcagga tttgatgact
    33361 atcacaaaaa gtggtttagt aaacgaataa actataattc gaatactaat atatttacag
    33421 gtgcagcaag caatgcaagt ggagataacg caaatatggt gcttaggtat atagtttctt
    33481 tttagttagg aggtatggaa gagtggcaaa tttagaaatt aaattatctg caaataagag
    33541 acagccttat ctacgtcacc gtgttgttgg tagagttggt gatggggggc tcacaacaat
    33601 caatgtacaa cttcttgagg aagatgaaat tacacctttt gtaattaatc taaacggtac
    33661 tttgaaattt gtgggcgaag tttcaaacgg taactatacc gagggagaac cagaaataat
    33721 cgattcgact aatgggttaa ttagttacac gttcactaag tcaaatttca gcacgagtca
    33781 tcaattcaaa caagcatatt ttgaatatgt agatcctaac ggcaaaaaag taacttttca
    33841 gaacttcatc atagacgtgt tagaacgagt ggatattaat tcggagcaag cgaaatacta
    33901 tatttcttca ttggaaaaat tacagagtga aatgcaaacc actttcaatc agttcattag
    33961 tgataaacag gtccaatacg atcaaatcta ctcgaaatat aacgaattag taagattgat
    34021 aaatgaatca gataagcaag taaatgatcg tattgatcaa accaatcagc aaatcggcga
    34081 tctcggcaag ctgaaaaaga tgtacagtaa cagcatcgac tttgggggct atgattattc
    34141 ggggaatccg aatttgttaa gtaagctatc atacgactta attgaaaatc aaaatacttc
    34201 agctggaaca ctttctaagg gtgaaaactc gtttaaatat aataagatat cagctgaagt
    34261 ggaaggtgga gtagagttat attataaacg aagaggtata gctaactggt taccctctaa
    34321 taaaacgctt gtaatgactg ttaagcttag agctggagcg gactatagtc cagttgacgg
    34381 aaaacttata ctgattagat ataggtatgt tgacagtgga actggcaaga ttgttttaga
    34441 cttacctatt aacagtaatt cgataactca ggaatggaaa gagtttagta ttactggaac
    34501 tactccaaca tttagcccac aagcatacca tccttggata caatttaggg ctcaagatgg
    34561 ggtacttggg gaaatagaaa tgagctatga catcaaaatc gaagaaggct ctacagctac
    34621 accatatcag cctaacttac ttgatgactc ttactggcta ggtaaaacac cgttgggtga
    34681 gaatttgata acgaatgcca agttcccaat tataacacga agcaatccta tttctggttt
    34741 tgatatttca gaagaattaa taataggtga aacttatacg gtatctttaa aagggactaa
    34801 gccagctact aaagaattcc acttatacta cggagaagca aattaccagc agttgcagta
    34861 tcaggcaact ttacttcctg tggaaggatt aacagatgtg tggagtgcca cttttacagc
    34921 cagaaatact agtgaaacga caaatttact tagagtagcg ttatggcaaa aacctaattc
    34981 tgcaacatat gatactgttc aaattgactg gctcaagatt gaaaaaggcg acacacgcac
    35041 accaaatatc agtcagttta aatactttgg tgaaggcttg aaagacagta acaatccgaa
    35101 cgactacagc tgggatgtca caccagagta tactgaaaaa agcttgaata atacggttag
    35161 tctgactgaa ccgcaatcca tcgaaggatt gaaaaatttc gaagatggta ttcaatcaaa
    35221 aggaaaatct gtattgacat cagacgacaa caaatatgaa gtcgtaacct taacagttac
    35281 aaacgggaat accggatcag caaagcttta tcgtgaagga aaaaccgtca ctatttattt
    35341 ttttgcgttg aatgggaaaa gcagtggtgg aaatgattca acgatactaa caattccaga
    35401 aggctatcgg ccaccaatta gttttgagca actggttggc tcgatagacc gttctacttt
    35461 gaacagtgct cagttatcta ttggtgcaga tggagccatt aaatggcgaa gaaactcaag
    35521 ttatggatcg gattatacct ttgcaattac ttacacgatt tagaaaggcg tgaatcgata
    35581 tgaaggcagc atatagacca attgaacctt acggattcga gcaaatcatt gtgaatgatg
    35641 aagaacattt accggaagaa tgcacagaag tcgaaccacc gattccaaat tggaaaccga
    35701 aattcaatta ctgggaggga aataaatgaa aaacatttgg aaatatggcc gtactggcgg
    35761 agagtatgca ggaaaagtat tggacgacat gcttgtatcc gttccttaca cagatcagcc
    35821 accgctcgaa ggggttcgtt cagatggcga accgctaacg attgctgatc agatgtttga
    35881 tcctaaattg aaccaatgga ttattttagc gaacgcgtta gatcacaacg atttaaacaa
    35941 tctcaaagcg atgtacgagg ctcttgaaca tgaaaacggc aacctaaaac agctaaatgc
    36001 caaactcatg ctaagcgatg tagcgattaa acaggaaaat actgcattga aagaaaaagc
    36061 tgacagttta gcacaaatca attcaaaaat gatgcttgct tcgttacaaa atagcaaaga
    36121 catttcagaa attaaagagc aactaaatcc agcttcaaag ggaggtgagt agtatgttta
    36181 gttttagcga tgtgaaaatg atgtatgatt ggggctgttt tactgacgat caagttcgtc
    36241 tattcgttcc actatgtatc acagatgaag aagcagatag aattattagc aaagaagaga
    36301 gcgcatctta agtgatgcgt ttttttgttg gaaagttggt ggaacatgaa agaagaagcg
    36361 ctccaagacg ttgtggagag attagtaaga attgaaacaa aattagacaa ctacgaatca
    36421 cttagagaaa aagctgatag tgcaaaagat ttggcagaca aagcctactc agtagcgcta
    36481 aacaacgcag aagatatcaa agaaatgaaa gccaataata aatggtcgtg gggttacatg
    36541 atcggtttag gcattacgat cattggctat ttcttgacta aattgtaaag gagttaagaa
    36601 gaaatgattt tacccgataa gtattatcaa gtcattaaat ggacggtttt aacagtatta
    36661 ccagctgcat ctgttttagt agccacgtta ggaaaagcgt atggatggaa tggaacagat
    36721 atgacagtac tcactatcaa tgcagtagca acatttttag gcgttatcac tggtgtgtct
    36781 gcatataact taaaaaaata ggaggaaaaa aatgaagaag aaaatcattt tatcattgag
    36841 cctaataatg gctctttttt tattgccaat taacgggttc gcctatacga tcaacaatga
    36901 atttaattta ggtgtaaatg aaggtagctc
//
LOCUS       DAFFSV010000013        36706 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFFSV010000013 DAFFSV010000000
VERSION     DAFFSV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.013"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..300
                     /locus_tag="LR110_000601"
     CDS             <1..300
                     /locus_tag="LR110_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL4821604.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(724..894)
                     /locus_tag="LR110_000602"
     CDS             complement(724..894)
                     /locus_tag="LR110_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4821605.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1088..1267)
                     /locus_tag="LR110_000603"
     CDS             complement(1088..1267)
                     /locus_tag="LR110_000603"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821606.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1277..1501)
                     /locus_tag="LR110_000604"
     CDS             complement(1277..1501)
                     /locus_tag="LR110_000604"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821607.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            1654..2766
                     /locus_tag="LR110_000605"
     CDS             1654..2766
                     /locus_tag="LR110_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL4821608.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2844..3137)
                     /locus_tag="LR110_000606"
     CDS             complement(2844..3137)
                     /locus_tag="LR110_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBL4821609.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3305..3619
                     /locus_tag="LR110_000607"
     CDS             3305..3619
                     /locus_tag="LR110_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBL4821610.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3640..5619
                     /locus_tag="LR110_000608"
     CDS             3640..5619
                     /locus_tag="LR110_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL4821611.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5632..5958
                     /locus_tag="LR110_000609"
     CDS             5632..5958
                     /locus_tag="LR110_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBL4821612.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5974..6492
                     /locus_tag="LR110_000610"
     CDS             5974..6492
                     /locus_tag="LR110_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821613.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6492..7844
                     /gene="celB"
                     /locus_tag="LR110_000611"
     CDS             6492..7844
                     /gene="celB"
                     /locus_tag="LR110_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL4821614.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7928..9433)
                     /locus_tag="LR110_000612"
     CDS             complement(7928..9433)
                     /locus_tag="LR110_000612"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBL4821615.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9525..10115)
                     /locus_tag="LR110_000613"
     CDS             complement(9525..10115)
                     /locus_tag="LR110_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL4821616.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10294..11940
                     /locus_tag="LR110_000614"
     CDS             10294..11940
                     /locus_tag="LR110_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL4821617.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11989..12708
                     /locus_tag="LR110_000615"
     CDS             11989..12708
                     /locus_tag="LR110_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL4821618.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13187..14134
                     /locus_tag="LR110_000616"
     CDS             13187..14134
                     /locus_tag="LR110_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4821619.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14146..15066
                     /locus_tag="LR110_000617"
     CDS             14146..15066
                     /locus_tag="LR110_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4821620.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15107..16564
                     /locus_tag="LR110_000618"
     CDS             15107..16564
                     /locus_tag="LR110_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4821621.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16909..17523
                     /locus_tag="LR110_000619"
     CDS             16909..17523
                     /locus_tag="LR110_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL4821622.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17516..19240
                     /locus_tag="LR110_000620"
     CDS             17516..19240
                     /locus_tag="LR110_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL4821623.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19237..20688
                     /locus_tag="LR110_000621"
     CDS             19237..20688
                     /locus_tag="LR110_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4821624.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21161..23314)
                     /locus_tag="LR110_000622"
     CDS             complement(21161..23314)
                     /locus_tag="LR110_000622"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBL4821625.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23316..24362)
                     /locus_tag="LR110_000623"
     CDS             complement(23316..24362)
                     /locus_tag="LR110_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4821626.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24512..25801
                     /locus_tag="LR110_000624"
     CDS             24512..25801
                     /locus_tag="LR110_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBL4821627.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            25814..28516
                     /locus_tag="LR110_000625"
     CDS             25814..28516
                     /locus_tag="LR110_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBL4821628.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            28501..29358
                     /locus_tag="LR110_000626"
     CDS             28501..29358
                     /locus_tag="LR110_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL4821629.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29429..30328)
                     /locus_tag="LR110_000627"
     CDS             complement(29429..30328)
                     /locus_tag="LR110_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL4821630.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            30497..32668
                     /locus_tag="LR110_000628"
     CDS             30497..32668
                     /locus_tag="LR110_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL4821631.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            32736..33077
                     /locus_tag="LR110_000629"
     CDS             32736..33077
                     /locus_tag="LR110_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL4821632.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33353..35107)
                     /locus_tag="LR110_000630"
     CDS             complement(33353..35107)
                     /locus_tag="LR110_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4821633.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35118..35954)
                     /locus_tag="LR110_000631"
     CDS             complement(35118..35954)
                     /locus_tag="LR110_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL4821634.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(35956..36639)
                     /locus_tag="LR110_000632"
     CDS             complement(35956..36639)
                     /locus_tag="LR110_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBL4821635.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        11844 a         6445 c         7481 g        10936 t
ORIGIN      
        1 gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa
       61 ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta
      121 acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc
      181 agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca
      241 atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga
      301 gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa
      361 tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg
      421 ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata
      481 aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt
      541 gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct
      601 ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca
      661 ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa
      721 aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc
      781 atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa
      841 ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg
      901 tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt
      961 ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc
     1021 ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca
     1081 aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag
     1141 tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag
     1201 cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca
     1261 ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca
     1321 ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag acacatggag
     1381 cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga
     1441 cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca
     1501 taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga
     1561 tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat
     1621 atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag
     1681 aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa
     1741 caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt
     1801 ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta
     1861 tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt
     1921 tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc
     1981 tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt
     2041 cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat
     2101 gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg
     2161 aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca
     2221 gttcttcttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt
     2281 tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt
     2341 agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt
     2401 gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca
     2461 gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt
     2521 attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat
     2581 agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg
     2641 gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc
     2701 atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat
     2761 cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt
     2821 gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt
     2881 aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt
     2941 tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt
     3001 ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt
     3061 ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata
     3121 ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt
     3181 gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat
     3241 attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg
     3301 atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc
     3361 aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc
     3421 tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca
     3481 aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt
     3541 tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt
     3601 gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg
     3661 ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct
     3721 gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta
     3781 gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta
     3841 caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga
     3901 gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt
     3961 ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc
     4021 agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc
     4081 gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt
     4141 atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa
     4201 agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca
     4261 agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct
     4321 ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa
     4381 gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc
     4441 gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag
     4501 tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg
     4561 aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga
     4621 ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac
     4681 ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat
     4741 ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg
     4801 gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg
     4861 attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa
     4921 ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact
     4981 ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt
     5041 ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta
     5101 gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag
     5161 caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac
     5221 gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa
     5281 gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc
     5341 atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag
     5401 gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt
     5461 gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca
     5521 atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt
     5581 gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa
     5641 aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat
     5701 gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta
     5761 gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa
     5821 cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca
     5881 caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg
     5941 tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc
     6001 gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta
     6061 cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca
     6121 gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta
     6181 ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat
     6241 tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt
     6301 acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt
     6361 gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc
     6421 aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag
     6481 gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg
     6541 cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga
     6601 ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag
     6661 gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga
     6721 cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg
     6781 aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg
     6841 cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt
     6901 ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt
     6961 taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt
     7021 gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt
     7081 ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca
     7141 atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt
     7201 ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg
     7261 cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg
     7321 tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca
     7381 acaattctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg
     7441 cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat
     7501 tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag
     7561 cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc
     7621 tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag
     7681 catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca
     7741 tcgcattcat tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag
     7801 aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg
     7861 aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata
     7921 aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat
     7981 ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt
     8041 gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa
     8101 cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt
     8161 caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc
     8221 caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac
     8281 ggccatctgg atgttcattt ttggcaaatt ccagcaatgt tttcaagctt agataattat
     8341 gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg
     8401 gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg
     8461 cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat
     8521 aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac
     8581 ctttatctga acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg
     8641 gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta
     8701 aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg
     8761 tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta
     8821 aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat
     8881 gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg
     8941 ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca
     9001 atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg
     9061 ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa
     9121 aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg
     9181 aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca
     9241 aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc
     9301 ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc
     9361 ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta
     9421 aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac
     9481 tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt
     9541 gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg
     9601 agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta
     9661 ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc
     9721 ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg
     9781 aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga
     9841 acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat
     9901 tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact
     9961 gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa
    10021 gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa
    10081 aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca
    10141 taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat
    10201 tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat
    10261 agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta
    10321 gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat
    10381 atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa
    10441 aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta
    10501 ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca
    10561 ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg
    10621 ctaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct
    10681 ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt
    10741 ccttgtatga gtgtgatacg agggtatttt caaggcaatc aagaaatgat gccttatgcg
    10801 ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc
    10861 atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca
    10921 tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg
    10981 tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg
    11041 ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc
    11101 ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc
    11161 aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg
    11221 gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct
    11281 gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt
    11341 tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca
    11401 ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga
    11461 ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag
    11521 gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc
    11581 attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct
    11641 tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg
    11701 cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca
    11761 aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt
    11821 cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg
    11881 gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa
    11941 gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat
    12001 aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt gatcaaaaaa
    12061 ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc
    12121 gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt
    12181 ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc
    12241 gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa
    12301 gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca
    12361 cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa
    12421 gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca
    12481 aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg
    12541 catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta
    12601 tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa
    12661 tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag
    12721 ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact
    12781 ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc
    12841 gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg
    12901 actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc
    12961 acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc
    13081 acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt
    13141 gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa
    13201 ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt
    13261 accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc
    13321 attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg
    13381 ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa
    13441 aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt
    13501 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
    13561 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
    13621 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
    13681 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
    13741 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
    13801 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
    13861 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
    13921 ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
    13981 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
    14041 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
    14101 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
    14161 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
    14221 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
    14281 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
    14341 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
    14401 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
    14461 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
    14521 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
    14581 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
    14641 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
    14701 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
    14761 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
    14821 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
    14881 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
    14941 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
    15001 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
    15061 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
    15121 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
    15181 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
    15241 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
    15301 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
    15361 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
    15421 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
    15481 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
    15541 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
    15601 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
    15661 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
    15721 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
    15781 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
    15841 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
    15901 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
    15961 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
    16021 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
    16081 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
    16141 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
    16201 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
    16261 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
    16321 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
    16381 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
    16441 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
    16501 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
    16561 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
    16621 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
    16681 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
    16741 ttggtaaacg gtgttcaatc agctgaccct cggtattcgc tcacaaaacg acaaaatatg
    16801 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
    16861 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
    16921 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
    16981 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
    17041 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
    17101 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
    17161 gctttattga gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct
    17221 attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg
    17281 attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg
    17341 gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc
    17401 gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg
    17461 aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa
    17521 taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc
    17581 ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga
    17641 acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat
    17701 gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga
    17761 aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt
    17821 atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct
    17881 ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta
    17941 ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact
    18001 agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt
    18061 tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg
    18121 actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa
    18181 aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa
    18241 ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc
    18301 tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa
    18361 aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct
    18421 ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca
    18481 aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa
    18541 ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat
    18601 ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa
    18661 tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag
    18721 tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca
    18781 agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta
    18841 tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa
    18901 gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat
    18961 tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt
    19021 aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga
    19081 agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa
    19141 tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac
    19201 aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg
    19261 gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta
    19321 ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag
    19381 cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt
    19441 attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa
    19501 gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc
    19561 gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa
    19621 aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat
    19681 gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat
    19741 aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa
    19801 gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg
    19861 ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat
    19921 caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca
    19981 ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat
    20041 gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga
    20101 cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa
    20161 cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt
    20221 atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa
    20281 aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa
    20341 aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac
    20401 cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc
    20461 atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac
    20521 catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag
    20581 atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc
    20641 gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa aaaaaagcca
    20701 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
    20761 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg gagttcaaaa gccagtttct
    20821 cggtattaag ccaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttaa
    20881 tactcgaaac taaacagctt tttcacgacc tctccactaa atggtgttca atcagctgac
    20941 cctcggtatt agctcaaaaa atggcaaaat atgaggaact attttgccat ttgtctctct
    21001 aatactcagg tcagaacgct gatttcacaa cctctcaata aacggtgttc aaaagcttgc
    21061 atctgcggta ataagaccaa ccaaacgaaa aatatgagga tctatttttg cttggctgtt
    21121 cttattactt gatgcaggac agctttttca caacctcttg ttaattatag tttgataagg
    21181 aaaaaggcaa atcttttgct tgatatttct tagggtgagg gatgatccct aattggtaag
    21241 agatcgtgcc gcctttcagc aaatcttgat gggtaaaata taatttcgta tattctctgt
    21301 ccatccgttt gattttatgg ataaattgtt gctgcggccg attaggcgtt gcttcaatcg
    21361 tcaattcctt tccatttgac aaaagcaaaa cagccttgtc aattaatggc ataccgataa
    21421 catattcacc ggttccaggc gtgacaggat aaaatcccag actattgaag atataccaag
    21481 cggccatact tccattgtcc tcatctccag ggtaacccat tggagaatcg tcgaaggtct
    21541 gagtcatcaa ctgtttaagc agtggctgtg ccatttcagg ttgtcctata taactgaaca
    21601 agtaaggata gtgaaaactt ggctggtttg aaatagctaa ttgtccaaat tctatcgcgg
    21661 ccatctcact catttcgtgg atctcaaaac catatcctcc tgggtcaaac gttgggggct
    21721 gattgcacag ctcaatgagt ttttctttat atctctcact gccaccatat tttccgatca
    21781 gtcctgaaaa atcgtgataa acagcaaagc tgctttgcca agcacttcct tctgcataat
    21841 cttgtcccca acgattagct gaaaatggtt ctctgaactt gccatcttta tcttttgaac
    21901 gcataaatcc tgactcatag tcaaagatat tttgatagtt caatgattgc tgctgatatt
    21961 tcttcgtcat ttcctcttct tctagtacct tggcaacttg gctaatacaa aaatcgctat
    22021 aagcatagtc taatgtgtga ttgactgatt cgtggtagtg attaggaaca tagccatatg
    22081 tcaagtaatc ttctgttcct tggcgtccat aatttggatg tttgctttga accgtagcac
    22141 ctttttccat tgccgttaga aattcaggca tcaagtctcg tcggatatct tttacagcag
    22201 catctgcaat gaccgcatcg atcaatgtcc caggcataag tccacgttca tcaggagaca
    22261 gccattttgg caaatagcca ctttctctat agctgttcaa aaaaccttcc agcatttctt
    22321 cgtatttttc tacagcaatc aaagaataaa gaggataaac cgttttatac gtatcccaaa
    22381 acccattatt tgtatagaga acaccaggtt ttatagatcg actagtcgta tcatagtgga
    22441 ttttttgccc actaggatca atttcataaa atgtttgtgg aaataaaaac acccgatata
    22501 aattatgata aaacgtccgc aattgttgtt gatcatggtg tgtgagctgt atacgatcaa
    22561 aataggactg ccattgttct tgactgtgag caaggtaagt ctctttgctt tggttttgtt
    22621 ctagttccag gttataagct gcttgttccg agctgataaa agatgtccca aatcggatag
    22681 tttgtttttt tgtctgttca aaatacaacg ggatcgagtc gctatttttg ttggaaacag
    22741 ctaactcatc aattgttaaa ggatattcaa atctcaatat aaaataaaaa gaaaaatcag
    22801 gatcttctga accagcaaaa tgtgtgattt ttccttccac tgtgtaagga tctataacaa
    22861 agagctgatg gcgtcccgga aaggataaaa gtaaaccgct gtcatttttg ctgtaatcaa
    22921 tagataagat tccgccatac atgctaggta tcaatgtaga acagatgcca taacgtaatt
    22981 gacagatcga cagatgagtc gggcaaaata tactttcttc tgggcggtaa gaactttgag
    23041 catggaaaag ggtgttctcc tgtagttttc cattgatcgg cgtaaacagc atatgactga
    23101 aatcacccat ccaaggactt ggttgatggg taagacggta gccttgaaaa acgcggtcat
    23161 caggatgaaa ccaccagctg ccgttttgat ccgtagtttg cggtgcaaaa taattcatcc
    23221 caaaaggaac gccagtataa ggctggcagt tcccattaga gaaatttggc tgattcgctg
    23281 ttgcatgtct agtatctatt tgaccgatat tcattttatt tctcctttgt tgattgtctt
    23341 ttgatgaatg aaactggaaa tttactatcg gatggtcgat cttttgtctc taaccaagtg
    23401 accaaagcat ctcccgcaag cttacctaac tgttcaaaat cttgtgcgat ggttgtcagt
    23461 ggcgggtcaa caagagctgc tgcttggata tcatcaaatc cgataattga aaaatcttca
    23521 gggatatgat aaccatgctg tctcaagaga ttcatggtct ggatagcgac aacatcgttt
    23581 tcacaaacaa aagcagttac ttcatactta gtgatataag aaagcaattc atctaatgtc
    23641 gcgcttttgt catccagtga agtgtggaaa gtcaaaccag ctttgtccaa tgcttcaagg
    23701 taaccaaggt aacgctgtcg aacggattgt ggatgagtct gcgtaccaaa caaataagcg
    23761 atttttgaat gtccctgttc gatcaaataa gaggcagcaa gcgcaccgcc ttggaaatta
    23821 tcggaaagaa cggtaggaaa aggtaactca tagatttttt tatctaatat gacgacaggg
    23881 aattctttca atgaaagctc aaaaagtaag tcaagatgtt gttctgtatc aagagcataa
    23941 taaatcaatc cgtcaaattc attgattagc tcttttgctg ttttattttg tagaaagtca
    24001 agcgtcgtca tcatgacatc aatctgttta tcttgcatca cagggttcaa tcccttgcta
    24061 aaatcaccaa cagacaaatc agcgacatat ggaagaagaa acagaatccg tcgtgttttt
    24121 gtaggagaag caagaatctg ttccggagaa gcggatttaa cgaaactgcc tttcccgcga
    24181 acgcgataaa taagtcctaa ttgctctaat tcagtcaatg cacgtttgga agtgattctg
    24241 cttactttat aggtacgtga aagttctttt tcagttggta gttggctgtc tacaggtaat
    24301 tcaccagtta aaatagccgt ttgcaaatca ttaaatattg tctggtacaa tggttgagtc
    24361 atataaacac ctcttaatga tatatcatat tcaaataata ccataatgga tgaaaaagta
    24421 aataaaaaga tattttttga aagcgtagac ataattggct gtatgaagta taatctttaa
    24481 agatatatca aatagaaaga aggttttatt agtggtttat aaagatatac caaaatcggt
    24541 gcagaaattt atggaagata tcacacaaaa atgtggggat atccatgcgg actgggcaaa
    24601 gaattttaca gcagcatttg ccaatactct gttgacaaca gtcaaaaagc aaaatgacgg
    24661 aaccaccttt ttattgacgg gagacattcc agcgatgtgg ttaagagact ctactgcaca
    24721 agttcgtcct tatttagtga ttgcaaaaga agatgaggaa ttagctgaca tgatcagcgg
    24781 gttagtcaaa aagcaatttt tttatatcaa tatcgatcca tatgcgaatg ccttcaacga
    24841 agaagctaac ggtgctggcc accaaacaga tcatacaaaa atgaacgatt ggatctggga
    24901 aagaaaatat gaaattgatt ctttatgcta cccaatacag ttggcctatc ttctttacaa
    24961 aaatacagga aaaactgatc aattcaatca atcttttatt gaaggtatca aaaaaatctt
    25021 ggacgtgttc aaaacagagc aagatcatga acactctcct tatacatttg tacgagacac
    25081 gacaagaaaa gaagatacgc tgatcaatga cgggcgaggt gcaaaaacag cttttacagg
    25141 aatgacatgg tctggtttcc gtcctagcga tgatgcttgc cgttatggat atctggtacc
    25201 atccaatatg tttgcggtgg tcgtgttagg ttatgtggaa gaaatttttc aaacgatact
    25261 cgatgataaa gaaattgtac agcaaacaca gaaattgaag aaagagatcc aagaggggat
    25321 tcagaatcac ggacgaacgg ttaatcaaaa agggcaaacg atttatgcat atgaagtaga
    25381 tggaatggga aacgccacag tcatggatga cagtaatgtg cccaatctga tcgccgcacc
    25441 ttacttggga tattgttctg aacaagagga agattatctg caaacaagaa gaacattgtt
    25501 gagtaaagaa aatccatatt tctatgaagg aaagtacgca aaaggaattg gatcctccca
    25561 tacgccagaa aaatacgttt ggccgattgc attagctatg gaaggaatga caacgtcaga
    25621 caaatctgaa aaagagcgga tattgaattt acttgtagca actgacgcag gaacacacct
    25681 tatgcacgaa ggattcgatg tgaacaatcc tgaaaattat acgagagaat ggttttcttg
    25741 ggcaaatatg atgttttgcg aattagtcat ggattacttt gatattagag tagaaaagta
    25801 ggaggtatcg taaatgaaaa agaaagtata tatcatttct catagccact gggatcgtga
    25861 atggtacatg gcttacgaac agcatcacat gcgactgatt gaattgatag acgatctact
    25921 agagctattt gaagttgatc cgtccttcaa cagttttcat ttggatgggc aaacgattat
    25981 tttagatgac tatcttcaag taaggccaga aaaaagacag gcaatccaac aagcaatcaa
    26041 tgaaggaaaa ttgagaattg gaccatttta tatcttgcaa gatgattttc ttatcagctc
    26101 ggaatcgaac gtgagaaata tgctgattgg catggaagaa agccgtaaat ggggaacccc
    26161 tgtcatgtta ggctattttc ccgatacatt tggaaatatg ggacagacgc ctcaattaat
    26221 gaaacaagct ggtatttcag cagcagcgtt tggccgtggt gtaaaaccaa ttggctttga
    26281 taatcaagtt ttggaagcag aaaactattc ttcccaatac tcagagatgt ggtggaaagg
    26341 ccctgatcaa acagcaatct ttggtctgct atttgccaat tggtatagca acgggaatga
    26401 gattccagta gaaaaagaag cagctcttgc cttttggaag caaaaattag cggatgcaga
    26461 acaatatgcc tctacgaacc acttattgat gatgaatggt gttgatcatc aaccggtaca
    26521 aaaagatata tcaaaagcaa tccatctcgc aaatgaactg tttccagatt acgagtttat
    26581 tcattctaat tttactgact atctggaagc agttcaaaaa gatgtgccag aagacttagg
    26641 ctcagtagaa ggagaattga caagtcaaga aacggatggc tggtatactt tagccaatac
    26701 agcatctgct cgggtttact taaaacaatg gaataccaag gtccagcgac agctagaaaa
    26761 tattacagaa ccacttgcga cgatggctta tgaagttagt ggaaactatc cccatgatca
    26821 attagattat gcttggaaga cattaatgca aaatcatcct cacgacagta tttgcggttg
    26881 ttcagtcgat tctgtccatc gggaaatgat acctcgtttt gaaaaagcag atgaagttgg
    26941 aaagtatctt gcacaagatt ccttagaaca gctgaccgca gcaatcgata cgaccggatt
    27001 cccaaaagac agctttccat ttgtcattgt aaatactgca ggaatggata aaactggtga
    27061 agcagaaata accatcgaat tagaaagaaa aagattcgct gaaggaatac ctgaacaact
    27121 atatcaagag ttagaaaatt tgcctaaacg taagtatcat gtagaaacaa aaagtggggc
    27181 cactatacca gctattttat ctgaagaaac tgtccaattt ggttatgatt tgccgaaaga
    27241 ccgtttccgt gtgccatata tggcaagaat gatcaaagtg actttaccat tagaaaacat
    27301 gccagcattt tcttgggaaa cattcgcgct tgtagaaggg gaagcgaaag cagaagaaaa
    27361 ggaaacaatg attcatcaga gtggacgaat catcgaaaac ggccctctac atttgacgat
    27421 tgaaaaaaat ggtaccatta caatggaaga tcgcaaaaac aaacgaaagt tgaataatct
    27481 gcatattttt gaagatatcg gcgatatagg aaatgagtat atctttaaac aacctttctg
    27541 tgatcagcca atcctatcca gtaacaaaga aaactctgaa gtaaaagtat tagtagatac
    27601 accggaaata gcaaaaataa gtattctaca ggaaatggaa atccctgttt ctgcagacga
    27661 gcgtttagaa aaagaacagc agatggttgt ggaatttcgt tatcgaaaag cagaacgatc
    27721 caaagaaaaa cgaatccttc aaataaaaac aatcatgaca atcagaaaag atagtaaaaa
    27781 aatcgacttt gaaacgactc tcgacaatca gatgaaagat catcgcttac gtgtcttgtt
    27841 tcctacaaag ttacatgtgg aacatcacga agcagatagt atttttgaag tggtgaagcg
    27901 cccaaaccat gtgtctaaaa gttgggaaaa ccccacgaat ccgcaacatc agcaagcatt
    27961 tgtcaatatt catgatgaag agtacggtgt aacagtcgga aactttggtt tgaatgaata
    28021 tgaagtaaca gaagacggac aaatcgcagt gacgctactt cgcagtgtgg gagaactagg
    28081 cgattgggga tattttccaa caccagaagc acagtgtcta ggtgagcatc ggttcaatta
    28141 cagcattgaa ctacatggac cagaagaaaa attctctact tatttgcatg catacgcagc
    28201 acagattcct ttttccactc aacaaatcaa acatcatgaa ggaacattga tttcaaaaca
    28261 acaatattta acgataaaaa gtgaaacgtt tgcaattaca gctttgaaac gcagcaagtt
    28321 tagtgataaa gtagtagtaa gaggtttcaa tatgtctagt catttagaaa aattggaaat
    28381 aactaaagac aatggaaaaa ctgtcatatt gaatctgctt gaagaaccaa cgaagcaagc
    28441 ggttgtccca ataatccaac cttatgagat tcggacaatc ggattcgagg aggaaaacga
    28501 atgtatggcg gaatagaagc aggcggaaca aaatttgtgt gtgcggtttc agatggtgga
    28561 gaaattttag aaaaattaag tatccctacc acaacaccag aagaaacatt ggatcaggta
    28621 tttgaatttt ttgatcgtta tgaattgaag ggaatcggga ttggctcatt cggcccgatt
    28681 ggtatcgaca aaacaaaaga taattacgga tacgttttgg caactcccaa aaaagggtgg
    28741 gcacaattcg attttcttgg cacaatcaag gaacgctaca atattcctgc tgcttggaca
    28801 acggatgtca atgctgctgc atatggggaa ttgaagcaag gagcagcaca aggaaaaaat
    28861 agttgtattt accttaccgt gggaacagga atcggtgcag gtgtcgtgat caatgaagaa
    28921 attttttcag ggatcgcaca tcctgaaatg ggacatattt gggtgaaacg ccatccagat
    28981 gaccattatg aaggtacttg tccttatcat aaagattgtt tagaaggatt agcagcagga
    29041 ccgtctattg aggcacgcac agggatcaaa gggcaagatc tgccgcaaga tcatcctgtt
    29101 tgggctattc aggcttatta tattgctcaa gcactgatca actatacgtt gacgttagca
    29161 cctgaaaaga ttattttagg cggtggggta atgaatcagg atcatctcct tcaaaaaatc
    29221 cgtcagcaat ttgttgaatt gatgggtggt tatatggaaa cacctcaagt aagtgaatat
    29281 atcgttcgtt ggggactgcc gaatgaaagt gggattatcg gtagtttgct gttagcagaa
    29341 cacgaacatc aaaagtaagc agacaaatta cacaaaaaga acagagtctt atcattatct
    29401 gctgataaga ctctgttctt ttttatttct attcccataa tccaggtaaa gctttaccga
    29461 ctattttttc ttgagtaaat ggatggatca tgtgcagttc taatgcatgg agcatcagtc
    29521 gttcagctgt tctattttgg tagagagggt ctccaacgat cggatgtccc ttatgagcta
    29581 aatgaactct gatctggtgt gtacgtccgg tttccaaaac acaatagact tgtgtttgtt
    29641 cagaaagtac tttgttgaca gtgacatgtg tcaaggcgct ctgaccattt ttatcatcta
    29701 cgaccctttt tctccgatca tggcgatctc gtccaatttt attggtgatg gtcatatctt
    29761 tggcaatttc tccccatacg atggcttgat atctccgata gatcattttt tgttccaaca
    29821 ttcttccaag tatcggcaag acaaatggat tttttgcaaa taaaacggca ccgcttgttt
    29881 ctttgtccaa gcggtggaca acgtaaggtc gttgctgttt agtgtcgaga taagcagcta
    29941 aatgattgag taaggtactg ttttctgtcg gttcatttgg atgagtcttc attcctgcag
    30001 gcttatccac tataatcaaa tgctcatctt catataaaac acggatgtgc ttcgcatctc
    30061 ctaattgaac ttcttgataa ttataatctg tttcttcgaa taacaatgtg atctggtcat
    30121 tgccctttac ttcatgatgg aacaaagcag gttgttgatt gatccaaacg tttttacgta
    30181 tacgcaaaaa gtgtcgaacc tttctaggaa ctaaccattc cttttcaagc aagtcgcgaa
    30241 ttgttgtttc aggatggttc ataggtaaag tgatcgtgat tttcacaagt gtactccttt
    30301 cagtgcaatt gcagactaat tgtaccattt ttaaaacata tttaagaaaa aacttacatt
    30361 tttctgttca ttcttatgtt actctacttg cactaacgta aaaaaacata ttgaactaca
    30421 acttaagcag gaagaaaaca ctctttcact atggtaaaat aagacgattg aatcaaatag
    30481 acaggagaag aagcaaatgg attttcaaag cattttagaa aagataaaag ctgctttgat
    30541 cagtttttgg cgttggatca gaccatatct cggacaattc catcgatggc gaaagcgcat
    30601 ctggaaaaaa tatcatgtga acaaattgat tttgctgatt ggtttgatct gtgtcttagt
    30661 gacaagtatc tacctcttta ttttagcaaa acaagcaaat gtcgaaacgt taaaatcagg
    30721 attaagccaa tcgacagtca tttatgataa gaaaaacgaa gaagctggca cactgtacgc
    30781 ccaaaaagga acatatgtag aattaaacgc gatttctccc tatatccaag atgcagtaat
    30841 ctcgacagaa gataggaatt tttacgagca tcacgggttt gatatcaaag ggattgcccg
    30901 tgcagcagtc cgaatggtct tgtctggcgg aacatcaggg ggcggaggaa gtacgatcac
    30961 tcagcagctt gccaaaaatg cgtatctgac attagaccaa acgtttgatc gaaaagcgaa
    31021 ggaattattt ttggcaatcg agatcgaaaa gaagtacagc aaagaagaaa tcttgaccat
    31081 gtatcttaac aatgcctatt ttggcaacgg ggtatggggt gtccaagacg cttccagaaa
    31141 gtattttgga gtagatgcaa cgaatgttac gctgtctgag gctgctactt tggcaggaat
    31201 gctgaaaggt cctggtattt ataatccgat cgatcaccca aaaaatgcga atgatcggaa
    31261 aaacacagta ctaagtgtaa tggaagaaaa cggaaaaatc acaaaagaac aagaacaagc
    31321 agaaagccag acagatatca gcgcgtatct taatgacaca tacgataatt cagattctgg
    31381 ttatcgttat ccttattatt ttgatgcagt gatcgatgaa gcagttaacg agtatggtct
    31441 tgatgaagaa gatgtcatga ataaaggcta taaaatctat acagctttag atcaggatta
    31501 tcaaaagcaa ttagaagcta cttatcaaaa cagtgcatta ttcccagcaa atgcttcaga
    31561 tggtgccatg gtccaatctg cttcagtagc tttagatccg aatacaggtg gtgtacaagc
    31621 actagtagga cgaagaggag atcatgtctt tagaggattc agctttgcta cacagatgcg
    31681 tcgttcacca ggatcaacga tcaaaccatt aagcgtatat acaccagcac tggaagctgg
    31741 atacaagcct tccagtatct tgaaagacga accgcaaagt tattacgatg cgcataattt
    31801 tgacgggaca taccaaggag aagtaccgat gtatcaagct gttgcacaaa gtttgaattt
    31861 gcctgctgta tggcttttgc atgagatcgg tttacaaaaa ggatatgata aagcagaaga
    31921 attcggcctg cctttagcaa aatctgataa gtattatggc ttagcccttg gtggactgga
    31981 aaaaggagta tctccattga tcatggcggg tgcttacagt gcatttgcca atgatggtca
    32041 gatgtatacg ccgcatctta tcacgaaaat cgttgattcg actggagcgg tgatcgtcga
    32101 taacacaaac aaaaaaccaa aacaggtcat atcaaaggaa acagccgatg aaatgaccag
    32161 catgcttctt ggtacatttt caaatggaac agcggctgct gcgaatcctg caggttatac
    32221 gatagcaggg aaaacaggaa ccacagaaac taatttcgat gccacaaaag caaatgacca
    32281 atggatgatc ggttacacgc cagacgtagt gatttcgaca tggatgggtt acgaagaatc
    32341 cagcaagctg cactatttgg aagggacaag tggaaatgtt gtcgggaaag tcttcaaatc
    32401 agcggcagaa ggcatccttc cgtatacttc aaaaacagga tttagtgtag cagatgccta
    32461 tgccacagga ggacaagtgg tagctgcaga tcaagttgga aacacaggaa attctaatgg
    32521 agagtctgga aattggcaag ataacttgaa tcaatatggt gaacaagcaa aagaagggct
    32581 gaaaaacttc ggcgatatgg tgaaagaagg cgttcaggga attggtgaag cggctaaaga
    32641 cctctggcgg aaatatcaag gagagtagcc cctttgaaaa cgaataaccc catgttacaa
    32701 tagagagtaa ctactaaaag aaaagagggc ataacatgtc aaacatctac gatagcgcga
    32761 atcaaatcga acgtgagatc cgtgaattac cagaattcaa agcgttgaaa gaagcgtacg
    32821 agaaagtaca agcaaacgaa gaagcacaca aactgttcaa agacttccaa gcgatgcaaa
    32881 tcgaattaca agaaaaacaa atgagtggac aagagttcag cgacgaagat gcaatgaaag
    32941 cacaagaaat ggctgtgaaa atccaatcag aagaagtaat caatgacttg atgcaaaaag
    33001 agcaaggctt cagtacaatc atcaatgact tgaaccgtat catcatgaca ccagttcgcg
    33061 acctttacag cgaataaaac tattttatga aacaacagag ggtgtgaaag agacgttcgg
    33121 ccttgagcga tgacaaaatt gcttcttatt gccgataggc tgtctcttga acaccattta
    33181 ccaagaggtc gtgagcctac cgtttaactc tgagttacaa ggaacaattt caaaaaacag
    33241 cttctcatgt ttgatgacta ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
    33301 tattcagaaa taggagctac gacaaaagtc ttgtcatagc tcctattttt tgtcattcat
    33361 ttttcaagcg gtagaaatag cctttttgag ctaacagttt ttcgtaagta cctatttcaa
    33421 cgatttttcc ttcatcgatc acaacgattt tatctgcctc tttgatcgtt gaaagccgat
    33481 gagccacaat aaaagtagtg ggtgtttctg tcaggtaatt cagggcttgc tggatttttt
    33541 tctctgactg gttatctaaa gctgaggttg cttcatctag caaaatgatt tttggctgac
    33601 gtatcaacgc tcgtgcaatg gagatacatt gcttttggcc accagacagc ttgttgccat
    33661 gttcgcctac taaggtatcc agaccatcag gaagctggtc aatcagtgaa gaaagttgcg
    33721 cagcttcgat gacttcatct aaacgttctt ttgatacgtt tttcaagcca taagtgatgt
    33781 tctctttgat cgaagcggag aacaacaaag tggtttgagg aacaacggct aaataatttc
    33841 gcacacttcg catatccagt tcatccaaag gctgatcatc tagaaaaatt tttcctgaag
    33901 tcggctggat aaatccaatc aataagttaa ggagtgtaga ttttccagag ccagatggcc
    33961 caacaaaagc aattgtttct ccagctttca cctctagaga aaaatgatcg ataatcggtt
    34021 tgagtgagtc cggataaaga aaagaaacat cctcaaaacg aaaatttcca tccaaatgtt
    34081 ggattttttt cttaccgtga taagtttcta catcgccagc aatcaacagt tcgccaatag
    34141 aagtgataga ttccatgcct tttgaaatcg taggaatcag cgtgatcatt ccagaaacgg
    34201 aattgacgat cgtcgtgaaa tacgtctgat acatcacgac atcgccgggt tggatgcgcc
    34261 cttgtaaagc taatatccca gtgaaaatca aacaaatcag ctgaaatatc tgaaaactag
    34321 cccagctgac tgatccgaaa acagattgca ggatatctaa gtggaatcct ttttcataga
    34381 tttgagtaaa atgcttattt aagcgagttg tttcgtgttt ttctaaccca tgagctcgag
    34441 caatcggtgc taattcgatc acttcgatca cttttgcaga agtctcttcc atttcttgcc
    34501 tgaagtctga gttggactgt ctgatccttt tacggaaaaa gagagtaacg aatgcggcga
    34561 gaggaacagt caataagaaa aacatgaaaa caacaggact tttataaagc gtgataccta
    34621 aagctacgaa taaattgaca gcgatattta atccgctgac aaatacttga gaggacaacg
    34681 tttggatagc ttctacatca cggatgatct ttgactggat tcgtccagat tgcaattctt
    34741 tttgataagg aatagatagt tcttggattt tcttgataag ggtcgctctt agtccagctt
    34801 ctacttgacg gatcgaagtg ctatggtatt ttacatgaaa ataattcgtc aatatatttt
    34861 gaccaatcag acaagccatc aaaaaagcgt tgaaataaat ggcctgcaac tgagaagaat
    34921 ctttcgatga agctgcattg attacattgg caatagcgat cggcattaac caagatggaa
    34981 gatgtttgat aataaaaaag agtcctgaca tggcaaaacg ccaataattt ccctgataaa
    35041 gatgcagcaa tgtctgaaaa gcatttgcat gatatttttg ataacttttc tgtattcgtt
    35101 cctccataat cttctcctta acaatcttgg taaataaact gcaagtgttc tattgcttca
    35161 ggaacataaa tctcaggagt agcttcaaga gtggcgtaca ttagaggacg ctcatatttg
    35221 atataatgca gtaccgggcg gaaatctaaa cagcccttgc caacaggtac catgcggatc
    35281 tttccatttt ccatgatata gtctttgaga tgaagcgctg taatataacc atgtaaattg
    35341 tccaaggcac ctcggatcac tttttcctga tcagcatgat tttctttatg catcaaattc
    35401 gcacaatcta aaatgatttt taaatttggt gaagctactt catcgattaa acgtttagct
    35461 aactgatagt tatagattgg atgattgatc cctgcttcaa cagcaacagt tacgccaaaa
    35521 ttttctgcat gagcgactaa ttcaatgata gaatttctgg cgatctgata agcttcttca
    35581 gtaaaatttt cttttgtgta accgacagag acactaccgg tttccgtagc aatcatcttg
    35641 gcttgatagg cagagcacag agcaatttgc tgtttgaaag ttgccaatgc ttgtgcacga
    35701 acttcttgat ttttgctgga taggttgata tagcatccta aaatggaaag ttcgatattg
    35761 tttttttgga agtattcacc aaaataggca gcactcccaa gtgtcatgtc agttaattta
    35821 agttgttcag gaaatgcttt ttgtggtgca aattggatat gggacaagtg aagctgtgat
    35881 actttttgaa cgatttcttc acgatttcga acatctaaat cgtgggcacg gatgcctaaa
    35941 tttaatggaa acatctcaaa ctcctttttg taaataatta gcaagaggca gacgggattc
    36001 ttttatcaat tcaacaatta ttcgggcaac tttatcagcg ccttctggac taaagtgggt
    36061 attgtctttt agtccttctg ggtaattttt acaagtatca ggcataagat gcaggtagaa
    36121 tttttttgtc tcttcttttg agaaggtctg gaacaattct tgtgatcttg agaacacatc
    36181 taaaagagga atatggtgtt tctcagcaaa tgcacgcatc gcttttggat agtctccaag
    36241 tatatctgga ttcaatgttc cgttttctaa gtaatctcgt cgcgtcacag atgtcagtaa
    36301 gaccatttgg acgttcgctt tttttgcgca ttcaaaaaat atctgcagat tttttaaata
    36361 gctaccgaag ggaagcgtac ctctttcttc ttggattttc tggtcgttat gtccaaattg
    36421 tacaaataag aaatcattcg gctgaaactg tgaagctaga cgggctaacc ttccttcctg
    36481 gataaatgat ttggtgctgc gtccattctt tgcttggtta ttgatctgga tctcgggtga
    36541 aaaataagca gagatttttt ctccccaacc tgtctcggga cgcttgtttt catctttttc
    36601 tgccgcagta gaatcaccgg caatatggat agtaggcatg ttatctcttc tttcataaag
    36661 gtttataggc aatgaagcag aacagcaaga gaagaagacg gagcaa
//
LOCUS       DAFFSV010000014        35613 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFFSV010000014 DAFFSV010000000
VERSION     DAFFSV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.014"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            265..2031
                     /locus_tag="LR110_000633"
     CDS             265..2031
                     /locus_tag="LR110_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBL4821636.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2133..3392
                     /locus_tag="LR110_000634"
     CDS             2133..3392
                     /locus_tag="LR110_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL4821637.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3539..4840
                     /locus_tag="LR110_000635"
     CDS             3539..4840
                     /locus_tag="LR110_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4821638.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4843..5694
                     /locus_tag="LR110_000636"
     CDS             4843..5694
                     /locus_tag="LR110_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4821639.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5707..6522
                     /locus_tag="LR110_000637"
     CDS             5707..6522
                     /locus_tag="LR110_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBL4821640.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6673..7839
                     /locus_tag="LR110_000638"
     CDS             6673..7839
                     /locus_tag="LR110_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL4821641.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8069..8716
                     /locus_tag="LR110_000639"
     CDS             8069..8716
                     /locus_tag="LR110_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL4821642.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8800..9744
                     /locus_tag="LR110_000640"
     CDS             8800..9744
                     /locus_tag="LR110_000640"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBL4821643.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9879..11330
                     /locus_tag="LR110_000641"
     CDS             9879..11330
                     /locus_tag="LR110_000641"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBL4821644.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11351..12187
                     /locus_tag="LR110_000642"
     CDS             11351..12187
                     /locus_tag="LR110_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL4821645.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12187..12846
                     /locus_tag="LR110_000643"
     CDS             12187..12846
                     /locus_tag="LR110_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL4821646.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12972..13661
                     /locus_tag="LR110_000644"
     CDS             12972..13661
                     /locus_tag="LR110_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821647.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13667..14623
                     /locus_tag="LR110_000645"
     CDS             13667..14623
                     /locus_tag="LR110_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBL4821648.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14667..15179
                     /locus_tag="LR110_000646"
     CDS             14667..15179
                     /locus_tag="LR110_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4821649.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15435..15983)
                     /locus_tag="LR110_000647"
     CDS             complement(15435..15983)
                     /locus_tag="LR110_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4821650.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16072..17253)
                     /locus_tag="LR110_000648"
     CDS             complement(16072..17253)
                     /locus_tag="LR110_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBL4821651.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17489..18997)
                     /locus_tag="LR110_000649"
     CDS             complement(17489..18997)
                     /locus_tag="LR110_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBL4821652.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19156..19347
                     /locus_tag="LR110_000650"
     CDS             19156..19347
                     /locus_tag="LR110_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821653.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19550..20467
                     /locus_tag="LR110_000651"
     CDS             19550..20467
                     /locus_tag="LR110_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBL4821654.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20460..21533
                     /locus_tag="LR110_000652"
     CDS             20460..21533
                     /locus_tag="LR110_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821655.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21681..22199
                     /locus_tag="LR110_000653"
     CDS             21681..22199
                     /locus_tag="LR110_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821656.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22180..22572
                     /locus_tag="LR110_000654"
     CDS             22180..22572
                     /locus_tag="LR110_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBL4821657.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22648..23583
                     /locus_tag="LR110_000655"
     CDS             22648..23583
                     /locus_tag="LR110_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBL4821658.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23716..24495
                     /locus_tag="LR110_000656"
     CDS             23716..24495
                     /locus_tag="LR110_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL4821659.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24488..24805
                     /locus_tag="LR110_000657"
     CDS             24488..24805
                     /locus_tag="LR110_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL4821660.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25049..26659
                     /locus_tag="LR110_000658"
     CDS             25049..26659
                     /locus_tag="LR110_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL4821661.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26816..27664
                     /locus_tag="LR110_000659"
     CDS             26816..27664
                     /locus_tag="LR110_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL4821662.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27895..28374
                     /locus_tag="LR110_000660"
     CDS             27895..28374
                     /locus_tag="LR110_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821663.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28407..30635
                     /locus_tag="LR110_000661"
     CDS             28407..30635
                     /locus_tag="LR110_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4821664.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30764..32407
                     /locus_tag="LR110_000662"
     CDS             30764..32407
                     /locus_tag="LR110_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL4821665.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32480..33025)
                     /locus_tag="LR110_000663"
     CDS             complement(32480..33025)
                     /locus_tag="LR110_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBL4821666.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33048..33533)
                     /locus_tag="LR110_000664"
     CDS             complement(33048..33533)
                     /locus_tag="LR110_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL4821667.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33523..34335)
                     /locus_tag="LR110_000665"
     CDS             complement(33523..34335)
                     /locus_tag="LR110_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBL4821668.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34474..>35613)
                     /locus_tag="LR110_000666"
     CDS             complement(34474..>35613)
                     /locus_tag="LR110_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL4821669.1"
                     /translation="ILKLTSTTGIPILYLMEQPALSVSQSFLDKNEISYMTHTSGTTG
                     IPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMM
                     PLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINN
                     QTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSAR
                     ITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHL
                     FLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWN
                     RWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        11654 a         6406 c         7118 g        10435 t
ORIGIN      
        1 atatacttct acgccaaaaa ctgttaaaaa acttttactt tagaaaattg gagaaattga
       61 tcgcttaaga taaattcagg acactaaata gtacaggaag tagactaaaa cttttaaaag
      121 ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa aaaactgtta
      181 ccgataacat ttttgaaagc gttgtctttt gcttctctat cacttatagt gatgacgtca
      241 tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg accggaaagt
      301 gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac taggaaaaat
      361 gatatcaaaa aagtatatgt gctatgtgga gatccatata ccataacaac tgaaaaatgg
      421 tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga ttattgggaa
      481 attgaagtga cgagcgaaac aagtcgctta cagtatgcgt tccatgttgt tggggaagat
      541 aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa tgtgattact
      601 gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt cactattcca
      661 gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc aaacggggac
      721 aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga ccgagaagac
      781 tttttcgggg gagatttgca aggtgtgatg gatcatttgg attacttgac ggaccttggc
      841 atcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa atacgatacg
      901 attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa aaacttgatt
      961 gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa tcatatgggt
     1021 gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat ctataaagac
     1081 tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt accagaaact
     1141 gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa actaaataca
     1201 gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat aaaagaattc
     1261 gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa
     1321 ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga gatttggcat
     1381 tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta tgcatttaca
     1441 gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga
     1501 atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt caacttatta
     1561 gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga attgatgaag
     1621 tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta cgggacagaa
     1681 atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg ggagaaggag
     1741 gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag gaaacagttg
     1801 tctaagatta tttctgaagg ttctactcag tggctaatag taaatgatcg tgaagataag
     1861 ctctatttca cacggaaact agagggacag atcatctatg tttactttaa tcagtcaaaa
     1921 gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg tcagttgtta
     1981 gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg aaaactgtta
     2041 ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa taagggtgta
     2101 acagcacgtt gaatacaaag gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg
     2161 gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc aatggtaact
     2221 cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt gattctggat
     2281 ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca
     2341 agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat gggcctgctg
     2401 gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta ggacagcaag
     2461 gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca gacaaagccc
     2521 aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc cttgttttgt
     2581 actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg gagaatctat
     2641 ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga tttttagcta
     2701 aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc tatgtatttg
     2761 gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa
     2821 tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa
     2881 gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca atcattgacg
     2941 gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta gccaaaattc
     3001 ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga tgggtagtca
     3061 gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta acgaatcagg
     3121 aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg
     3181 aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa tatgaaacag
     3241 ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga
     3301 tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa
     3361 caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc atttttataa
     3421 aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat tatcttgctc
     3481 ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg gcattcagat
     3541 gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt cacttattcc
     3601 agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt tcgccatatt
     3661 cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag gattggttac
     3721 tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag gcactttgca
     3781 attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag acgcttatcg
     3841 agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg aagtcataca
     3901 taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc ttctcatgat
     3961 ttttacgatc attttcccag tcctagttac attgttcact gcatttacta attatgactt
     4021 caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact tttttagtat
     4081 cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg
     4141 gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca
     4201 ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac catgggcggt
     4261 cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg taggtgccat
     4321 caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca gtgcaatctc
     4381 ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg gctggttagg
     4441 atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag aagacttata
     4501 tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc
     4561 tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga atttcaataa
     4621 tttctccatg atctatctgt ttaacaatgg cggtcccgga agcgtaggcg gaggagctgg
     4681 ttctactgat attcttattt cctggatcta taaacttacg actggaactt ctccgcagta
     4741 ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg tgtcattgct
     4801 ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa atttcattcg
     4861 agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct tttaatactg
     4921 tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa gtcaggaaat
     4981 attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt tactcggcta
     5041 ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc tgtcctaaca
     5101 atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg ctatcgtttt
     5161 atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc gactatggca
     5221 gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa gtactggttc
     5281 ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat gaaaggatac
     5341 tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc agggcatttc
     5401 aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt tcaagcatta
     5461 tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct tcgaacacct
     5521 gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac aaaccaaaga
     5581 gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct attattcttc
     5641 tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg ataggagcag
     5701 agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc aactcaagtg
     5761 tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt atttttagtt
     5821 tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc gattccatta
     5881 gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa attttctgaa
     5941 cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt gacccctgaa
     6001 attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac ttttattgat
     6061 tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg ccgctatacg
     6121 atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga tttccaacga
     6181 tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc ttatagttta
     6241 agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc ttcctttttc
     6301 ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga gagtgcaaat
     6361 ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg gtttatccac
     6421 tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct gcttttatgg
     6481 gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta agcgatttat
     6541 gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt tctgaaaaaa
     6601 ttaaacttct tagttgattc ttttcttttt tagtattaac gtaatagggt agttttttta
     6661 aggaggaaaa tcatgttaga gaaaacttta tacaatcaat tattttcacg ttctttctcg
     6721 ctccctgtcg aagtaaatta ttgggacggt acaacgaaac aatacggaga tacggacact
     6781 cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat tacaagtaac
     6841 gcatctttgg cactaggaga agcctatatg gatcatcgaa tcgagatcga gggaagtatc
     6901 caagcattga tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg aaaagattat
     6961 ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga agatatccat
     7021 gcgcattatg acttaggtaa cgacttttat aaactttggc tagatccaac tttgacttat
     7081 tcttgtgctt atttcaatac acctgaagat acgttggaac aagcacaaat caataaagtc
     7141 catcatatct tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga tatcggttgt
     7201 ggctggggaa cattgatgtt cactgctacc aaagagtata atgtcaaggc aactgggatc
     7261 acactaagtg aagaacaata cgatttgatt accaaaagaa taaaagaaga acatttagaa
     7321 gataaatgcc gtgtattatt aatggattac cgcgaactaa aaggcgaaac gtttgatcat
     7381 atcacaagtg taggtatgtt cgaacatgta ggttctgaaa atcttgaagg ttatttcaaa
     7441 gtggtaaaag atttattgaa accaaaagga acagctttga tccatggaat cagccgacaa
     7501 caaggcggtg cgacaaatgc ttggatcaat aaatatattt tccctggcgg ttatgtccct
     7561 ggtgttactg aattagttga taatattaca aaaaacgatt tgcaactgat tgatttggaa
     7621 agcttacgtc gtgactacca attgacatta gaacattgga caaagaattt tcatcaagtc
     7681 caagatcaag tcacgaaaga aatgggtgat tccttctatc gaatgtggga tctatattta
     7741 caagcttgtg cagcttcatt tgaatcaagt aatatcgatg tgattcaata tttgcttgtt
     7801 caacctggaa acaatgacat cccaatgcat cgtgcataat tgaggcaaaa gtactgttta
     7861 aagactattt aaaaagaagc taagttttat gatgctccag aagtgaactt ttttgtctaa
     7921 cttctaggat tgtctctaaa gcttagtttc tttttcttta tattttcata ataaaagttt
     7981 ggttctttaa tttttaaaca tgacagaaaa ttttttcttc gttatacttg taatagaata
     8041 ctattttata acaaagggga tcgaatcaat gaaaaaaact gttatttata ctagtttatt
     8101 tgcagctttg tttttagctg cttgtcaatc aaataccaat caaggcacat ctgggaatac
     8161 gtcttccagt agcgaacagc gctcttcaca ggtatcggac tcctcaacaa aagcaagcac
     8221 atcttcgtct agccagacaa aaagttctag tgaagcaagt actcagtcag atatcaaagt
     8281 gtctgcagct cgtgcgatcg aattgttcca agataaatac ccagatactg caattactag
     8341 tttggaatta gatagtgatt ggggatctta cttctacaag atagaaggtg tcgatgatca
     8401 aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa atggagacag agaatccaga
     8461 aactctagat cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc tggatgttga
     8521 taaattgatc acgattgagg aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac
     8581 ggcaacagat tgggatttgg ataaagattt aggtactact tattgggaag tcaaagtaaa
     8641 agacggcaat caaagtacat cagtcaagat caatagccaa acaggcgaag tactgtcaac
     8701 tgaacaagat gattaagaat ttcttcggaa aacaagagtt gatttttctt ttagtcatga
     8761 tacactagac aaaataactt gtttgaagga gcgatttact tgagtttaga aacgaccttt
     8821 gccaatcata tctttgccaa tcctttgatg aatgcatctg gtgtccactg catgactacc
     8881 caggaattag atgaactggc tcattcagaa gcaggagcat ttatcactaa gagctgtacg
     8941 atcaatgaac gaaaaggaaa tcccgaacca cgctattttg atgttccttt agggagtatc
     9001 aattctatgg gacttccaaa cttaggtttt tcttattatc tagagtatgc tcttgcgtat
     9061 gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt ccattgccgg gatgagtgtc
     9121 caggaaaact tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa
     9181 ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac tagcttacga ttttgaggcc
     9241 acttatgaaa cactgaaaga agtattcagc atcttttcta agccgctagg aatcaaattg
     9301 cctccttatt ttgattttgc tcattttgat cagatggctg atatcttgaa tcagtttcca
     9361 ttgacctatg tcaatgctat caacagtgtg ggcaatggtt tgtatatcga tacagaacaa
     9421 gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca ttggcggaga gtatatcaag
     9481 ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc
     9541 attggtacag gtgggattcg gacaggacaa gatgcatttg aacatctgct atgtggcgca
     9601 agcatgttac agattggtac cgagcttcat aaagaagggc cggaaatttt ttctagaatc
     9661 ataaaagaat taacgcagat tatgtcagaa aaaggatata cgtcaattga cgaatttaaa
     9721 ggcaaattac gcaccatttc atagcaaaga gacgaaggtc atgattgatc ttcgtctttt
     9781 tttataaaaa acactcgtct gttcattcct gtgcaagcgt actcattgta tgttagacga
     9841 acttgtggga aaataacaga ggcaaaaagg aggataatat gaatgatgca acagaattag
     9901 caaaagagct taaagaaaaa gaatttagtt ttcaagaact agttacggat atacagaaaa
     9961 aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta
    10021 attcccacga gctattgatt ccccagtcag aaaaagggcc attggctggt atcccatttc
    10081 ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc tacttcagga tctcgtttat
    10141 ttgaaacaca ccgagcttct catacatcta attatgtgcg gcaagcagaa gcgataggtc
    10201 tagttccttt tggacaaacc aatgcgccag agtttggttt caaaaatatt acagacccag
    10261 tcatctatgg cccagcaaga aacccttgga accttgaatg tacaccgggc ggatcaagtg
    10321 gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt agctggtgct agtgatggcg
    10381 gtggttctat tagaataccc gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag
    10441 ggacgatgcc agttggtcca tatgcttgga gaggatggca aggagctgct attgatttcg
    10501 gcttgacagt ttctatgaga gacacggagg ctttatttta tgggatgaga agtatccatt
    10561 ccggtgcgcc atatcaagta tcgccagttg agtggcaaac acatccaaga aaaaaacggt
    10621 tgaagatagc tttttgtgtt gattcaccga tccactcaca gattagtgaa gaggcagtac
    10681 ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg gcatgatttg acagagattc
    10741 cttacccact agatggtcgt aaactgatca cgtcttatta tcagatgaat ggcgcagaaa
    10801 cagcagcaat gatccgctcg attgaaacag gcatccagcg agctgtcaga aaagaagaac
    10861 ttgaacccat ttcttggact ttgttccaat atggtcagaa gctctctgcc gctacttaca
    10921 tcgattctct gcatgtatgg gatgaagcag caattgtcat ggaaaaccta catcaagaat
    10981 acgatctatt cctttcacca acgactgcat ttaccgcccc taaaatcagc gaagatttgc
    11041 aaagtgatgc gataagagca cagatggaac acatcagtga ttgttctgaa gtagaaggtt
    11101 tagacgttgt ttcttccatg tttgaggaga gcttgaagat cacaccttat acacagttgg
    11161 ctaatctgac tggacaacca gcaatcagct tgcctactca tttgacaaaa gaaaacatgc
    11221 ctctaggaat ccaatttatg gctgccagag gaagagaaga tttgctattt caaatcggct
    11281 atctatttga agcaaatcat cagttccatt taccaaatcc atggaaatag cagaaacaag
    11341 gagaagaaga atgaagaaaa aaagattgtt tgctctaata actagtgcct tttttatttt
    11401 aagcgcttgc ggtacagctg ccgctaatca ggaagataac agctggagta atgtagaaaa
    11461 aaagaaagaa ttagtagtag cgacttctgg cacactgttt cccgcatctt attataatga
    11521 tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa gaggtggcta aacggttaaa
    11581 tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa atcacttctg tttcaaaaag
    11641 agaatcagat tttgctgcga acgattttgg tatcacaaaa gaaagagaaa aaaaattctc
    11701 cctttcagag actttcaaat attcatttga cagtatgatc gtacgaaaaa aagatgattc
    11761 aggaatccac tcacttgaag atcttaaagg gaaaaaagca gctggcgaac ccaatactag
    11821 ctatatgcgt ttagcggaaa aacacggagc taaattagtc acatatgaca atgccacgaa
    11881 tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat gttattctaa atgattatta
    11941 tctgcagaaa atggccgtat ctgctttacc agatatccct gtaaaaatac tggagaacgt
    12001 ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa
    12061 gaaaatcgat ggcgtattaa aagagatgaa agaagatggg acgacaaaga aaatatctga
    12121 aaaatatttt caaactgatg tttcagtcaa accgaaagaa acgatggctg agtcggtgag
    12181 tgagtgatgt actttcctct aattgatttt tccctcatgt ggacttcact tcctttcgta
    12241 ctgcaaggct tggtatacac attagggatt ggcttcgtgt cgtttctttt aggaaatctg
    12301 gtagggttgc ttttgacagt tttaggtctt ttggattggc tgcctctgaa tgtatttatt
    12361 cgtttttatt tatcgttctt tagaggcatt cccgcactag tcctgctgtt tctgttatat
    12421 tttgggttac cttatcaatt aagtgcctta acggcttcag tcatctgctt tacgattacc
    12481 agcagtgcat ttattggtga aatctatcga ggatcactag caggcgtttc ttcaggtcag
    12541 tgggacgctg gtttagcttc tggtttttct ttctttgggg tattgaaata tatcgttttg
    12601 ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg tagctatgga tctagtgaaa
    12661 ggtacttcgt tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc aaagatcata
    12721 ggcggtcgga cattcgatta tttttctctc tatattttag ttgcactgat ttattgggtc
    12781 atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa aacgttctat gcatcagtca
    12841 atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat ttctattttt tgtttcttgt
    12901 atattctgcc tagtatttta aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg
    12961 aggtagaaaa catgaagacc ccgctaacca tcgaaataga atcctcgtta taccaatatt
    13021 gcatcgagca aggcggcttg gttgtagaag aagtcacgat gcctgatgac caaggaattg
    13081 tagatacgct tagctgcttg acaaaatttg atggaagccg agaatggcga tgctatgagc
    13141 tgaaagtgac aaaagcagat tttcgctcta cagccaaact gtcatttgtt ggacattata
    13201 attattttgt tttaccaagt aaactatatg aagaagtaaa aactgaaatc cctacaggaa
    13261 taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc tctggtacat
    13321 ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt accggaagaa gctttgacaa
    13381 accgcttcat ggcttcttta ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg
    13441 taaaatattt tccaacagat caattgtaca aagaattgaa aagacgttca gaaaatcaag
    13501 atccattttc taacgaacga tattatgaac ggtttttaga ggaaatagct agtcaggcaa
    13561 tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac
    13621 aacagcagat ccgtcggttg cctacagagc cattggagtg aaaaatatgt actatcctta
    13681 tttaagagga aaacaatttg acctattagc attaaaagaa gcactgtcgc gaggattgct
    13741 ctccaataaa atccaaccag tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt
    13801 gatcgaatta tttcaaaaaa agcaccatcg aatcgctgtg atccaaaatc cccaagtagg
    13861 acaattcaaa ttattcgatc agcatgtgca cagttggaca gtcaaagaaa atagctctgt
    13921 cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa gtactggatt cacctccagc
    13981 gtttcttgtt ttcgatggac agcactaccc aaaagataca gaggtgtgga aacagttggc
    14041 aggagttgat tcgaaatttt tgataccaga tactagtcgc tttcgcatct ggctgcccga
    14101 aaacaaaatt gtgatccgtg actcttttca aacgagaaag catgttgaaa gttatgcaga
    14161 taaaacagat gactttttta gtgatgacta tctttttttc cacgcagacg gatatattgg
    14221 attttctgat ttcacgatcg aaggtagccg ttatttcgat aaagggttcc ctagccgagc
    14281 gcttgctatc catctaacgt atattgatgc ttacggcaat atacgcgtga aacattttgt
    14341 ctctgacagt aacgactctg ctaaagacca agcattgaag tttcttgaag caggagataa
    14401 aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg attacttcag gaatgattga
    14461 attattggct ctttaccagc aacaaaaatt ccctggactg ggagtgttga aaaagtggtc
    14521 acttatgcat catttggaac ttattagtca cctgctagat catccaaaag attggcttaa
    14581 atattattca aaagttcagg aactatatga agtgatccaa tgattagata atgtaaatga
    14641 aaaaggaata gaaaagagga aactggatgt atattcggca aacaaggaaa gaagaagtag
    14701 aagaagtggt agaaatcatt aatgccgcag cggatttcat tgcttcgcaa ggctctcctc
    14761 aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag
    14821 aaagctatgt attaatagtg gaagatcaaa ttgctggaac agctgcatta gtctcaagcg
    14881 tggatcccgt ttacacaaaa atagatggta gttggaaaga atcaactggt ccttatttat
    14941 cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca aaaccgttct
    15001 tagaacattt gttgacgatt gcagccagca aaaatatcca agatatccgt atcgatactt
    15061 atccagctaa tgaaccgatg gaaaagacca tctattctgc tggattcact tatcaaggga
    15121 tgattgaatt tccttttgaa cacggcgaaa gaaaagcata tcaaataatc atcaattgac
    15181 tataaaatca tctgagcaaa gataaagaca ttaacactac tgaaaaaaac aatcatatga
    15241 tagccaagag gttgtaacag aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa
    15301 tgcttctcaa atttttgtgg aattttagcg tattttacga aatagctgct ttctgaaact
    15361 cccttcatcg agaggtcgtg acatagcttt tgtcacgacc tctcggctta tcctacgagt
    15421 attattcgat acatttatat caataatcct ttttttattt tatctgcagc ttcttggaac
    15481 gctccatcgt ttgacacgac taaaagtgag gggatagctt tcaaacgttt caactgtgct
    15541 aaatcttcta cataaaccgc cagtctggct gaaatctgtt cagatgaatc tccacgtttt
    15601 ttcaggcgcg attgcaatgt ttctttagag atcgtaataa agataggacg gactaccgtc
    15661 ccgaaatact catataaatg ccaaaaacca tttgctgtca aaacggcgta acaatttcct
    15721 actgtcagtt tctttttaat ttctgcttta ctggaaccat atttatgttg agcatattgg
    15781 tcccactctg caaactcatt tcccttgatc atctgattga atgtattgtc agaaacaaaa
    15841 taataatcct cttgatcttt ttccccaggt cttggtgatc gattcgtgta agtaatgatc
    15901 ttttgttccg gctgaaaaat agcttctgcg agtgaagttt ttccagaacc agaaggaccg
    15961 ataaatacat agcaataatt cattgtcatt tctcctttga tgattgaaca agtgaaaaag
    16021 ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc ttttttgtat attataccag
    16081 caagccagtc acaatagcac ttaaaaagct gactaaagtc gctgttagca aaattttcaa
    16141 tccgtgtttt gcgacaagat ttcctttttc ttcatttaat cccttcatcg caccagaaat
    16201 gatcccgata gaagagaagt tggcaaatga aacgaggaag acagatataa ttgctgttgt
    16261 acgctcagta aaatggaaat taccatttgc taattctgtc atagcaacaa attcatttgt
    16321 aaccagttta gtcgccatga tactaccggc atccactgct tctttccatg ggattccgct
    16381 gatgaaggca agaggggcaa agatgtaacc taggatctct tggaaagtga tgccgaatat
    16441 cccgtggaag atagcattga tcatggcaat caaagcaaca aaaccgataa gcatagcagc
    16501 aaccgtgatc gccacatgga aaccatcaag aatgtattct cccaacattt ggaaaaatgt
    16561 ctgttctttg ttttcttcaa tgatcagctc atcttctttt tcttctaata catagggatt
    16621 tacaattgaa gccagaatat aaccgccaaa taagttcaaa acaagggcag tgatcacata
    16681 tttagaatcg atcagtgcca tataagatcc gacgatcgac atagaaactg tagacatagc
    16741 cgatacactc atggtgaaca aacgtttctc agataggaag ggtaattcct tttttatgga
    16801 aataaaaact tccgattgtc ctaaaatagc agaagctact ccattataag attccaattt
    16861 gcccatcccg ttgattttac ttaatcctag accaacagct ttcataaata atggaagaat
    16921 cttgatatag cgtagaatac cgataatagc agaaataaat acaattggca tcaatacgga
    16981 aagaaagaaa ggtgcacttc ctttatttgc tacaccggca aaaacaaagt caacaccttg
    17041 cccagcaaaa gcaagaagat gatcaaacat ttttgccaat acagaaacaa ccgctgtacc
    17101 aaaagttgta cgtaataata cgaaaccaag aacaagctgt aaaacgaaca tcacaattag
    17161 tggtttcaat ttgattgctt ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac
    17221 aaataaacca ataataccta ataaataact caaataatca ctcctattcc aaattccgtt
    17281 ttattttacc tgttttgcaa ataaaatact aatctttagt gaaattttca ttactttttt
    17341 agagtgaaac gcattcattt tccattagaa aataaagcga acaaaataaa aggtttaaaa
    17401 agtgtaacgt cttagtaaag tctataaaaa aacgaaacta tcgattaagc taatagtttc
    17461 gttttcaaca tttataagta aactttttct attggtaata tagattttcg gaagggtaga
    17521 caggtgcgca ggtaatatcg attcctagtt ttttcaatgt ttgttcatca tttttattga
    17581 ccatcacagt agagtgggct tgtgctccct caagttcagc tagtttatca taagcaactt
    17641 gagcgattgg attcgttact gcactgattg ataaagcgat caagacttca ttgatgctta
    17701 gtgcatccaa agaggaatga agtgtctttg atttcaatcc ttgtattgtt tcaagaacta
    17761 atggtgacaa taaaggaatg tcatctgcga tatgggctaa atgttttact gcatttaaga
    17821 taactgccgc tgacgcatcc atcaatgaac ttgtacgccc ggtaatgatg acttcatctg
    17881 gtaattcgat tgcaatcaca gcagaaggat cttttgtagg actttgcgct tgaatcttat
    17941 ctgcatagtc atgggcaggg actaccacgt ttcggtcttc aggacgtaaa ccgacttctt
    18001 ccataatcag tttgattcga ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc
    18061 attctgtttg gaaataacga cggatgattt cttgtttaga ggcttcttgg acaacggtat
    18121 catccacgat cccaaatcct acacgattca cacccatgtc agtaggagat tgataaacag
    18181 attctttacc agtaattttt tcaataatac gtttgattac aggaaatgtc tcaacatccc
    18241 gattatagtt cacagctact ttattgtagg catcaaagtg gaaggaatcg atcatattga
    18301 catcttttag atcgactgtg gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa
    18361 cattccatac tgggaaagtt tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt
    18421 catgatacag ttggttcaag gaagtagcga gctttccact tcctgggcca ggagctgtca
    18481 ctacaacgat tggtttagtt gtttcaatat actcattttt tccaaaacca tcttctgaga
    18541 cgattttttc tacgttgact ggatagtctt cgatttctga gtgtttatag accttgatgt
    18601 ttcgtctttc tagtttattg ataaagactt tcgtggcagg ctgtccacta tagcgagtga
    18661 taacgacact gttgattaat aagccataac cacgtaattc atcgattaac cgcaagatat
    18721 ccatatcata agtaatccca taatcaccac ggatcttatt acgttcaata tctccagcat
    18781 agacgcaaat cagtatttca gcttgatctt tcaatttttg cagcaatttg attttcgcat
    18841 cttcttcaaa tcctggaagt acacgtttcg catgcttgtc gtcaactagc ttcccaccaa
    18901 attctaaata taatttgtca tagtggttca ctcgttccaa aatatacgct gactgctctt
    18961 caatatattt ttctgaatca aaaccaattt ttttcactag gttccctcct taagtactaa
    19021 agcatttttt catttttttg aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac
    19081 aaagaaaatg gtttaaacca acttgtctag atagttattc attggtatga tagtttttga
    19141 taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa acgcatataa acactatatc
    19201 cgttcgattt ttaccggtta tgaaagaatg cttgaagatc ccgaattgga acaattgacg
    19261 tttacttatc taaacgaaga aacgcagcta actcgtgatg agcatcagcg cattcatttc
    19321 acaacaagag atctgcctag taaataaaaa ataagataaa ggtcatcgga ttcgattctg
    19381 tcctatcaga tggaaagact agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc
    19441 ctgttttatg gagcattgcc cgctcttttt tataacaatt ttttacagaa aatggtacta
    19501 ttgaagtatt aataagtgaa aaacagatga gtaagaggag gcaagtagta tgaaagagcc
    19561 agatacaaat gcaaattatg agccagacga actagaggag acgaatgcgg atcaggatac
    19621 aggacaagaa tcgtcttctt tttacgatat caaagaaaaa gcaagcatga atctttccca
    19681 attattagaa gacttacagg cttttgaaaa agctgtccgc gaagaagata tccctgagat
    19741 ctaccgttta tacaatggcc gattgaatga agaattaaaa agaagctcaa atgaaaatca
    19801 cgagatcgat caattgctga tgcgtaagat ccatgatcgt tttttacagg aatttccttt
    19861 tatggaacaa gtagaaaaca tctcgccaac gatgagctat tacaaaatcg gcacttatta
    19921 ccatgatcgg cccacggtcg gtattgatgc aagtctgcca gaaatttttg ttttgcctaa
    19981 gatcgatgaa gagtgggaga aatattccca accggagtca acggactatg acaaaaaaat
    20041 caatgagcta gacgcaaagg tgatcactgc tcagacagaa attgaacgtt tagatgaaca
    20101 aataaaagaa atcaatcgac aaatgactga tttgaacgac aataaaggat tcttgaatcg
    20161 taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag aagcaagtac tatctaatga
    20221 aaaattaggc tggcttcctt atattgagac gcctgaaaca atccagcagc aaaaagaagc
    20281 attgaagcaa gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag
    20341 gcaaatcaga cgctatttcg gtagcaaaga gggattcggt caagcaatcc atgaattctt
    20401 gatgaattat cttggcaatg agaatcctaa taatcaatct aatgaaggag gttccgagta
    20461 tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga acaaacagaa gatcatgaag
    20521 aaacgaacac ttcattggaa gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc
    20581 ctatgctaga ggaaacaaaa atacacgaaa aagaaaaagt aactccgacg aatacgaata
    20641 ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag
    20701 agcaagatca tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt
    20761 tacgtatgag aaaagatttg atcgaatcag attttccaca attattagaa tcatatgatc
    20821 gttttatatt gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg atcgaatact
    20881 tcacgtatga attactgaaa ccgttgaatg atattggtct ggaacggtca aattcagctt
    20941 atgacacatg gaaaacaaga catttttcga tcaattatac gttgaatgga caaaacgaat
    21001 tagtcttttc ctttgtatta ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc
    21061 tcctaaacgt atcaccagag acaatggaaa tcaatgtcca agatgaccgg gtgctttctt
    21121 tgatccgaca ctggtcagtg gatcgagtat tctctgcagg gcagatcacg atattcaatc
    21181 acaaattgaa tcaattatta gcccatgcaa gaacacttgg ttttaccgtt aatcagacat
    21241 tactagacaa tacgaagcca cttcgtttga atcttcagag cgaatttgaa ttgacgaatg
    21301 aagtattgga tgatattttt atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga
    21361 tcaacgaaac tacttatcaa gtacgattag ataaaggaca gtctttaacg ataacaacaa
    21421 acgaaaaaga tcaaacagtc ttgtcgatct catctggaga ctacccacga tctgtcattg
    21481 atttctttat caattacgaa ttcttggtcc cattaatggt gagaaagtcc tgattggata
    21541 agttggatta ccagatgtca ttcagataat tctgttccaa aggacttgcg gcagttatcc
    21601 agaataattg tcacaagtct tttctgtatg tactaaatgg acgtgaataa cttgctgatg
    21661 aaggagggat ttcattagga atgtccacaa ataaaatagc acagcttagc ttattgtctg
    21721 ctgcttgtgt agcaggtagg atcgcttttc aatttattcc gaatttccag ccaatgactg
    21781 ctattttttt atttatagta ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc
    21841 tttctattgc gatcagcagt ttttattttg gtgttggacc atgggtcatc ggccaatgga
    21901 tcagctatac tgttgttctg agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa
    21961 ggaatctatg gttaaaggga gtctgttttt ttctggcggg catggtttac ggaataggta
    22021 tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc ttggatctat taccttcaag
    22081 gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt gttctttctt gtttttctgc
    22141 cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt
    22201 ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt gcagtacaac ctatacacaa
    22261 tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa
    22321 gaaaatggtc aaaacttttc ggaaaaagaa gtgttcttca aaaaaggcga tgatttactg
    22381 agtattttga aacgaaattt tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa
    22441 gggcactcac aggatgagaa aaatcaactc tattggatgt ttactgtgga cgggaaaagt
    22501 gctactactg gtgccaaaga gatcaaattg aatgatggac aatcggtcat tttcaatttg
    22561 tctaaactat aagtttcata aaaaactgtt aaagggcgaa tattgccata attcagtgag
    22621 ttcagttgat gaaagaagga aaataacatg gaaaatttta aaaaaagcgg aatgttttcc
    22681 gtaattgctg ctctaggggc taatattcta gttgctatca gcaaatttgt cggctatgca
    22741 atcagtggca gtgccgccat gctaaacgaa agcatccaca gtatcgttga ttgcagcaat
    22801 cagattttgc tgttattcgg tgataaacaa gccaataaag gacagagcga gttgcatcaa
    22861 tttggtgaag gacgagcaaa atactttttc agtacaatcg tcgcaatgat gcttttcttc
    22921 ggcggtgggg cacttggggt gatggaagct tttgaaaagt tgacgcatcc atcccatgaa
    22981 gtgggtaatc cgatcattgt tatagcaata ctgttatttg gtttggtaat cgaaggaagt
    23041 tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata ctgaaaaact ttccacgatg
    23101 cgctttttgc gcgaaagtcg acacagtgaa atactgatta tttttacgga agacttgtgt
    23161 gctgtaatcg gtttggtctt ggctttagca ggaacaatat tgacttatgc aaccgggatt
    23221 gctgcatttg atgcgatcag cggattgctg atcggtttct tgctgatggg agccgccatt
    23281 ttcctagcaa aagaatttta tagtctgatc atcggtgaaa gcgtgacagc aagcgattta
    23341 tcaaaaatca agttagcttt tgaacgtcct gagatcagtc ggctgatcga tataaaaaca
    23401 gttcatttaa gtccaacgga gattttagtt gcagcaaaag cagatatcgt tggtaacaaa
    23461 gagaatgaag cttactcggt catcaatgat attgaaaaaa atatgcgtca gtctcttcct
    23521 gacaagaaga tgtatatata cattgaaaca gataaatatg acccaaatta ttctcgtaaa
    23581 taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa tataatcttg tttttaaata
    23641 aaacggctac aatttagcga atttatgtta caatgagtcc gctgtttatt tgttgtaatt
    23701 aaaggagata ttttaatgaa caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca
    23761 gtgatgccac tttgtataag ctatatacca gtaggattag cttgcggagt attgttgcaa
    23821 aaagttggat ttgatcctct cttatcagga ctgttatcga ttctggtctt ttccggtggc
    23881 gcgcagtttt tagttgcatc tatgcttacg acgcaagcat catttgcaac gacattattg
    23941 atggttttct ttttagaatt acgctataca ttattaggag caagtcttgc cggttttatg
    24001 aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat cactgaatga tgaaaactat
    24061 gcggtgaact acttaaagtt ttcaactgac ccttcctgga ataaaagaaa ggctttatat
    24121 gtcaattggt tctcaatgac tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc
    24181 tcggtgattc gagtagatgc tgaccttgtc cattttgctt tgacggccat gttcattttc
    24241 atgtttatca tgcagatgaa aagcgcacta ctgatcttta caggtttatt ctcaggcgta
    24301 ttaggggtcg tattcatggt attattccaa aatactttcg gattgattgc agcaacagtc
    24361 attgcttcat ttgccggatt tatgctggag aaggcattca aaaaagaaaa agctaagaaa
    24421 ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca acttcccgga acaggaggcc
    24481 aatcaccatg actaaactat atttgctact tttagttgct tgtctgttca tcgtggcata
    24541 tatcccgcgc ttgttcccga tgctttattt tactcatcga aaagtgccat cctggttcag
    24601 tgactggatg aaatatgtac cggtggcatt atttgcggcg ttagctttta aagatgtttt
    24661 cattactcat gaacatctgg atattgcttg gaatatcaaa atagctgcaa tgattctagt
    24721 agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca gttttgacag gacttgcgtc
    24781 tgtctttttg ctgtctatgc tttaaactgt tttattttat taaaaacaga gattgcgaca
    24841 taagttgttc aaactgatct atccgaactg catttgcaac agtcaattct ggatgcaagt
    24901 ttattcgtag ggataaaaaa gattagtgtt ggcttatgat gcaagctctt ttttatttta
    24961 ggaattagag agggatttgt ccttctatga ttttttgcta taatagggaa gatgtttatt
    25021 ttatgaaaga atagttgagg gaatttaaat gaataaaaaa ttgatgcaag gaacattttg
    25081 gctaaccttt gctaatcttc tatgtaaagt tttaggggta gtctatttga ttccctggct
    25141 aagtatgatg gggaacaatc aagatggtat gcttgctacg acattgtata atgttggcta
    25201 tcttccttat ggattgttcc tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa
    25261 aaaagtagct gtagccacaa aagaaggaga taacgctgct tgtcggctga tttttagaag
    25321 tacgatcaat atcatgtttg tcatcggtat cgcctctgct gcactgatgt atttatttgc
    25381 tcctttacta gccggaatca gtccgattgc taacgttaat aatgggatac tagctatcag
    25441 aagtttatgc ccatctttga ttgctatccc aattcttagt gcaatgagag gatatttcca
    25501 aggaaaaaat gatctgcggc cttatggaac ttctttgatt atcgaacagg ctgtgcgagt
    25561 gatcgttatc ttggctggta cttattactt gcgcgtattg actgacggaa ccatattaga
    25621 agctgtattg atcagtacag tcgcttcttt cttcggtggg ttagctgcga ttatccatat
    25681 gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa gattttttga tttccagccg
    25741 ttatttcgaa agagaaaacc gatctgcctc tgtctctatc attcgggaaa cactaccgtt
    25801 tatttttgtc ggctcagtca ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc
    25861 actgcttcac tttttccgtc cagagatcgc agatcagcaa ctggaattct tattcagccg
    25921 tgcttctgtc aatccaaata aattgacatt gatcctgatc tccatggtag gaacagtagc
    25981 tataagcagc ttgccaatat taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa
    26041 aacagtagga gatagttttt cgattgcgtt attgattctt cttccttcat tgactggaat
    26101 gtctttatta gctggaccac tatacacatt gttttttggc tatgatcctg aaagtgtcgg
    26161 atatttccaa atggctttgc tagcttcttt gtttttctct ttattcacga tcctatctac
    26221 gatgcttcaa tctttgaatc accatcgttt tgcgatccga ttaactgtag aagcaattat
    26281 cctgaaagtc atcttccaag taatcggatt aggattagtt ggcggatacg ggatgagctt
    26341 atcaaatggc ttagcattcg gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga
    26401 ataccgaata gcacctttag caaagatcag caattttttc ttaaaaactt tccgctccac
    26461 cctgattatg cttgcagtat gttttgccgt tttctttttg ctgaatcagc gattgtcaat
    26521 ggaatcaaaa agctatgcaa tgatttattg tgtggctgta ggaagtattg gcggcttagt
    26581 atttttattt tctcaattcg gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca
    26641 tagcagaagc aaagaatagg ttaactaatc gttttttgct gtcacttgaa aaacggcaac
    26701 tcgtatgatt acgcttgtcg tttttctatt ttccaatttt agtttcatat ctaatcttac
    26761 ttatgatata gttataaggt ctggaaaaat tctttgaaac gaggaaatta aacacatgaa
    26821 acttgcagta gttacagata gttcggcata tctgccggaa cgtatcaaga accatcccga
    26881 cttattcgtt attccaatcc ctttgattat ggatgggaaa atttataacg aaggaatcga
    26941 tatcgaagcg gatgaatatt atggactttt aaaaaacagt aaagattttc ctactacttc
    27001 acaacctgtc gtaggggaag ttttgggttt atatgaggaa ttgaaagaga aagggtatga
    27061 cacgatttta agtatccatc tttcttcagg gatttctggc tttgtcaaca cattatttgc
    27121 gatgaaagat gacatcagcg gagtaacggt cattccttat gactctaaga tcactagtat
    27181 gccgatggga cacatggtag aagctgcctt agacttaaat gatgaaggtc agtcccttga
    27241 tgaaatactt gcacatatcg atcgaatccg agataatacc tacgcttatc tgattgtaga
    27301 tgatttgaac aatttagtac gaggaggacg tttgacaaat ggtgcagcgc tgattggcag
    27361 cttattaaaa atcaaaccga tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa
    27421 aatccgttca agtaaaaaag catttgctcg agctgaagaa attattggta gaagaaataa
    27481 cgaaatacaa ccgccagtca aactttacgt gatacatgca aataatttag aagtagcgat
    27541 tgcggagaaa gagaaattgc agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt
    27601 cggaccagtc attggtacac atttaggtga aaaagccatc ggcattgcca tttcagcgca
    27661 ataattcctt ttattcaaaa aacataccaa aaatattcca acatcatctt ggactttcta
    27721 catgttacta atgagcgtgt aaaagaacga gatggtgttt ttatttctat caatgaagct
    27781 ctttttcttt gcaatcctca cttcaatcct ctataatgtc gcatatagtt tagtaaatga
    27841 gtaaactact aatttactta tttactaaaa ataaaaggat gaggagttaa aataatggaa
    27901 ataccaacaa atttaaaaca taaaccagta atcgttgtag aagactatga taaagttgat
    27961 ggaagaaatg cattaaatac ggatgcaaaa gggctttcac tgggccttgc tcagtggaat
    28021 gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac atactggaga aaaatggtca
    28081 cgacagtcgg aagaattacc aatcacacgt attttagatt tagcgatctt agctgcacaa
    28141 ggaagtttat acttccaaga tgcttatcgt tatgaaaaat tttatgatcc agaaaatcca
    28201 gtcgtagaca tcattggatt acaagggaac cgaatgacgg tagaagtaga taccaaaaat
    28261 ccaaagatcg atgaagacat cattttgtat tacgatacac tacaaaaaga cggagaactg
    28321 attggggaac gattccgtat tttaaaacgt cttttagatg agctaggata ttaatagaca
    28381 gagatgatca aaaggggaag agaagcatgg agaatttctc actaacagaa aaccgaacct
    28441 tttcacggct agaaaagcaa atgatctgga agaaaccgaa aagccatcgg acaagtgcag
    28501 aagaatggcg gatcgcctcc gaaatcaaag ggaattggca agatcctgaa atgaaaatca
    28561 gtaacgtact tttggaagga gatgccgggt cggggaagac acaattagcc aaagcactat
    28621 ctgctgatct gcaacttccc tatacaaaag tgacctgttt tgcagatatg gacaaatcag
    28681 atgttttcgg agccttatta cctgtagtag attctaataa ggaacaagac caagaattac
    28741 tagaagctat ttaccaaaca gatacacttg aagctgtact gacactgatc cagaaccatt
    28801 acggcattga tcctttttct gctaaagaaa aattagcaga gctagttcaa aggatcgaag
    28861 ctgattatcc agatgcaatc cattacagct attacccttc tgaaatcgtt cgagcgattg
    28921 aaaaaggata tttattagag attcaagagc cgactgtcat ccgagatgcc tctgttcttg
    28981 tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa tttaccgaca ggaatcattc
    29041 gtcgccatcc agattgcgtc attgtgatca ctacaaatcg aaattaccaa ggaaatcgcc
    29101 cattaaatga atctttaaga gatcggatgc aacatgcgga aaaaatggat cttcctgcct
    29161 tagaagtcat ggcagaacgt gcgatggcga aaactatgat ccaaaaacaa gaacttctct
    29221 taaagatggc agagattatc cgtttgttgg atgaaactgc aaaagctaac gccatcaaag
    29281 gtgtagcagg aatgcgttct tatttttatt gggcaaatac ctataagcaa ggacaagatt
    29341 tactccagtc gatttatcca aaagttttgt ataaattatc cactgattca gatgaattgg
    29401 caattcttga acaagctttg tctgacagtg gattgctact ggaacttcag gacatattga
    29461 gaaaagaaaa atggggtcat cctcaatctg agcatacaaa aggaagaacc attagcgccg
    29521 aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga aattctgcgc ttagatgaaa
    29581 agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga gaaacaagaa gaaacaatag
    29641 aaaaaaacga gattcctcct caaatgcaaa aagaacgcag tagcgatatc gaaggaaaaa
    29701 atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat gtctgcttct gacatggaag
    29761 cacatgataa ggaaatcaag aagcaactga ataaagaagc gcgacaaatc atgaaggaaa
    29821 cgattcatga aaaagaaggt ctcatcgttc accgaccagc tttccaagga aaaacgccgg
    29881 aagcacgtga gctacacatg caagtaatgc caatagtaga atcacttagc cggcaaattt
    29941 tagagttact ggataatgaa caaacagaga cgtaccaaaa aggaaaatac gaaggtcagc
    30001 ggttcaatgc aagcaaagta gcatatggtg atttgagaaa ttttgacaaa aagaacccgc
    30061 cacatgagca accttcttta gcggtagctg tacggatcga tgaatctggg tcaatgatac
    30121 gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc cattgccgaa ttttctaaga
    30181 aagtcgatat cccattgttg atttacggag atacagcaga ccgttcgtca agagaaaaga
    30241 cttctttgtt ttcttataaa gaatttgaag acggatttca atggttaaat gaaaaattag
    30301 tcactatgaa accaaggcaa aacaatcgtg atggtgctgc acttcgttta gcagcagaaa
    30361 aattaaacag acaatccgca acaaccaaac tgttgcttaa tattagtgac ggtcagccaa
    30421 aagcgttgcc ggattataca ggcaaaaaag caaaagaaga tatccagtct gttttgaaag
    30481 aatatgaacg tcaaggaatc ctatttgtct ccgcagctat tggccaagac aaagaagaaa
    30541 tcaagtcgat ctacggtgaa agtcgttttg tagatattac tgacttgaat gaatttccca
    30601 agcaaatgat tcaactgatt gcccgctatc tatgataaaa taaagctcaa cgaacgcact
    30661 tagcagattc gttgagcttt tcgtttctag ttaccggtaa caaggatact tctaaaatag
    30721 aatcggttat aatcaagagg gaccgttaaa ggagtgaaac gatatgatac aagaacaaaa
    30781 atggtggaaa gaagcagtag gctatcaaat ctatcctgct agtttcaaag atagcaatgg
    30841 cgatggtcaa ggagacatca atgggatccg tgaaaaactt aattatttga aagatcttgg
    30901 tatcggcttt atctggatca atccgatcta tcaatctcca tttgtcgata atggttatga
    30961 cgtaagtgac tatcaagata tagaagagag attcggaacg atggaagatt ttgatttatt
    31021 gctaaaagaa gcacatgaat tagggatcaa agtgattatg gatcttgtca tcaaccatag
    31081 ttcggatcag catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc cgtatcgcga
    31141 ttattacatt tgggtcgacg gggtagatgg caaagaaccg aataattgga catcgatttt
    31201 cggcggttct gcttgggagt attctcatga aactggacag tattatctgc atgtttttgc
    31261 aaaagaacaa ccagacttaa attgggaaag cgagaaattg aaagaagaac tattcaatat
    31321 gatccgatgg tggttggata aagggataga tggatttcgt ttagatgcga tttctcatgt
    31381 aaaaaaagat gaatattcag taaaagccac agagaaccct ttctcaccct ttcaaaatgt
    31441 ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt tttgaagagt acgatatcat
    31501 gactgtcgga gaagcaagtg gtgtcactgc agaagaagga ccacaatggg tagggaaaga
    31561 cggttatttc gatatgatat tcgaatttga tcatatccat atctggcagc aagaaaaaga
    31621 agggcaatta gatgtcctta aattgaaact tgcattaagc gcttggcaaa cttcattgga
    31681 tggtatcgga tggaacgctt tatatatgga aaaccatgat gttcctcgtg ccgtttcagt
    31741 gtttggagat actcgtcccg atttttgggc tatgtctgca aaagcaatcg ctatgatgta
    31801 tttctttttg caaggtacgc catttattta tcaaggacag gaaatcggaa tgacgaatat
    31861 gccctttgaa tcgatcgatc aagttgatgc agtagattcg aaaagacttt ataaaaggct
    31921 gcttgcggaa ggtaagacaa gagaagaagc attggatatc attcgtgaaa caacgagaga
    31981 caacagcagg acacccatgc aatggacttc ggaacaatat gctggttttt ctacacatga
    32041 accttggctg atcactaatc caaataccaa aacgatcaat gtagaacaac aagaatatga
    32101 accagagtcg gtcctgcaat tctataagaa catgatccgc attagacaaa ccaataaagg
    32161 tctgatatac ggttcttata aagaatatct tcatgaacat ccacaacttt atgtctatga
    32221 aagatattta gaagatgaac aatatcttat tatggtaaac ttaacagaat cacttgctga
    32281 ctatgaactt ccaaaagaag cagatcagtc ctggacatta ctcttgtcaa attcctctag
    32341 cggagaattt gaagctaaag gcatactagc accttatgaa gctcggttat ataaaacaaa
    32401 caagtaaaac ttcctcgtta aagttcatta gttggacttt gcgaggaagt ttttcagatt
    32461 gctctgtcaa ttactcattt caataattcg ctctaggacc accgatatat ttaggacgag
    32521 agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa acgaggtctg acaggtactt
    32581 ggacatgttt gatatgcata ccgattgatg tgtcaccgat atcgataccg gcttgtccaa
    32641 ctacgtgttc cacttcgaca ggatcattga actggtcaaa agcagcaaca gaacaagcac
    32701 ctccagcatg tagtgcagga acaacagaga caatttcgaa attttttgct tctgccactg
    32761 ttcgttctac tactaatgct cggttcaaat gttcacaacc ttggacagct agcgcaattt
    32821 ctttaggatc caacaactct tttaacgtac ggatgatcca ttgaccaact tccgcactgg
    32881 aattttttcc aatatgtcct ccaacgactt cgcttgtgct gcatcctaaa acaaaaagat
    32941 cacctttttt caagtgtgcc tcagccaata tgtcattaac tatttcagtc aattggtctt
    33001 ttaattcttt ttctgacaac ttcattttct aaaaccttct ttttcaatta atattgcttc
    33061 cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg caagtcccat tccaatcaga
    33121 ttttggaaga tgtttcctgg tatagaagcc actccagccg gccaatcata taaaatccaa
    33181 ccggcgatac catagccgat gatcataact aagcttccta ataaaagccc taatccccac
    33241 atacgagtag aagatttgct gaaagaacca gcaacatatc cttgtaaccc gtggatcact
    33301 aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat cgatcaatag accactggaa
    33361 gcaccaacaa ctaatccacc cgttggacca taaagaaaag cagcggtata gatcccggcc
    33421 tcgcataatg tcacaaatcc attcgtagcc ggtacaggga tcaaaattgt taatgaaagg
    33481 gcaactgtca acgcaatcag catagcatag atcgttaatt gcttagtttt catttctcat
    33541 tcctcctttt tgaataccgc cagaccagac acttccggta tgatcgttca tcagcacgcc
    33601 attagaaata caatgggcaa caaatttttt actttctgct actgctttgg taaaatctgc
    33661 ccctaacaca agattcgctg ttattgcaga agaaaaggca caaccggccc cgtttactgt
    33721 tgtagtgtcg atttttttat gagaaaatac ctcataatga tcaattgaat agagaagatc
    33781 cacagcttca tctccagcca tccttgtgcc gcctttgatc acaatgtttt ttgttcctga
    33841 agcagtgagt tctttagcga gatggatcat ttctgttaac gaagagggaa gagcttgacc
    33901 agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc gtaacaagtg gaaaaagttc
    33961 ttcgatgagc tgcgaaatat acaattgatt gatcacagct tcagtttctt tgaatgccaa
    34021 aacagggtct aaaatgattg ggatattttg ttttgttttt atgaagtctc gaacaaccat
    34081 gattgcttca cttgaattca ataacccaat tttgatacca tctaatgaag tcattttatc
    34141 aatcgaaact aactgtcgtt taagcaattc caaagatata gattccagca taaatcgctc
    34201 attaatatct agtgccccta tagaagtcaa ggcgcttagt ccgaatagac caaaattctc
    34261 aaaagttttt aaatctgttt ggattcctcc accagcaaat ggatcagaac cgccaattgt
    34321 caagattctt ttcataacat tttcctccat gaataagagt ataacgaaat tttcaaaaaa
    34381 gaaaaacagc caattggcat atttttatct catccaattt aaaaaaacaa aaagaaaaga
    34441 aagaccaaaa tggatctttc tttccttaaa tagttaggac ttttgtgatt ttaattcttt
    34501 ctcgatttga agccgttgca ctttcattgt agctgttcgc gggattgcat caaaatcacg
    34561 gatgataggg acgttcaagc gaggaagttc atggacttgc tcccaccatc gattccaatc
    34621 catttcttta tcaggagcta aagcaataat cggttgtgga gaatgatttt tatcccttac
    34681 aatcactact tctgccaaaa aatctaacga atctaataaa aagtcttcaa tagctaatgt
    34741 actattgatc gtttcgatca aatccacttg ccgatctttc aaaaataggt ggccatgctt
    34801 atccataaaa ccgtaatctc cgctatccca ccaatcgcca taaacatttt cctggaaccg
    34861 tgcatcttcc ttgtaataag tcaaagcccg tcctttagac agtaattgta tatgaccatc
    34921 tgtgttttca ggtaggacat tcccaactga atctgtaatc cgagcgcttg tcatgtcttc
    34981 cagtccaacg cccatatctc ttgcatcact tgttttcaaa ctctctagtg tatgagcctt
    35041 aagaatcatt ggtccgcatt cactttggcc atatacttga agaaataccg catcctgtgc
    35101 catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt atagcatcaa aagtggagtg
    35161 atagtatcgg atacccgaaa aagcttctgg atgctcctta gcagtaaaac gccactgaac
    35221 aaagttattc ggatgggtct ctagtgcaat tggccggtga gcttccaaca tatgaacaac
    35281 ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat cccatggaca tcaaagaaga
    35341 gataccaata ttgaatctgg aatgcactgg agagatgtga aaagcgatta atttcttttc
    35401 cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc catcccatag aatgagcaga
    35461 atgacaaata agtttaggga taccagttgt accagaagta tgggtcatat aagaaatttc
    35521 atttttgtct aaaaatgatt gagaaacaga caaagcaggt tgttccatta aataaagaat
    35581 aggaataccc gtggtgctag ttaattttaa tat
//
LOCUS       DAFFSV010000015        35437 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFFSV010000015 DAFFSV010000000
VERSION     DAFFSV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.015"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1152
                     /locus_tag="LR110_000667"
     CDS             <1..1152
                     /locus_tag="LR110_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBL4821670.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="LR110_000668"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="LR110_000668"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBL4821671.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="LR110_000669"
     CDS             2728..3003
                     /locus_tag="LR110_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBL4821672.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="LR110_000670"
     CDS             3009..3776
                     /locus_tag="LR110_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBL4821673.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="LR110_000671"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="LR110_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBL4821674.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="LR110_000672"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="LR110_000672"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBL4821675.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7919..8221
                     /locus_tag="LR110_000673"
     CDS             7919..8221
                     /locus_tag="LR110_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBL4821676.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8224..9387
                     /locus_tag="LR110_000674"
     CDS             8224..9387
                     /locus_tag="LR110_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL4821677.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9413..12841
                     /locus_tag="LR110_000675"
     CDS             9413..12841
                     /locus_tag="LR110_000675"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBL4821678.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12862..13986
                     /locus_tag="LR110_000676"
     CDS             12862..13986
                     /locus_tag="LR110_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821679.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13976..14413
                     /locus_tag="LR110_000677"
     CDS             13976..14413
                     /locus_tag="LR110_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL4821680.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14397..14735
                     /locus_tag="LR110_000678"
     CDS             14397..14735
                     /locus_tag="LR110_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBL4821681.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14854..15414
                     /gene="rsmD"
                     /locus_tag="LR110_000679"
     CDS             14854..15414
                     /gene="rsmD"
                     /locus_tag="LR110_000679"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBL4821682.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15411..15902
                     /gene="coaD"
                     /locus_tag="LR110_000680"
     CDS             15411..15902
                     /gene="coaD"
                     /locus_tag="LR110_000680"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBL4821683.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15892..16935
                     /locus_tag="LR110_000681"
     CDS             15892..16935
                     /locus_tag="LR110_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBL4821684.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17094..17822
                     /locus_tag="LR110_000682"
     CDS             17094..17822
                     /locus_tag="LR110_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBL4821685.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17938..18183
                     /locus_tag="LR110_000683"
     CDS             17938..18183
                     /locus_tag="LR110_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL4821686.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18180..18398
                     /locus_tag="LR110_000684"
     CDS             18180..18398
                     /locus_tag="LR110_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBL4821687.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18534..19073
                     /locus_tag="LR110_000685"
     CDS             18534..19073
                     /locus_tag="LR110_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBL4821688.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19066..21339
                     /locus_tag="LR110_000686"
     CDS             19066..21339
                     /locus_tag="LR110_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBL4821689.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21377..22423
                     /gene="holA"
                     /locus_tag="LR110_000687"
     CDS             21377..22423
                     /gene="holA"
                     /locus_tag="LR110_000687"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBL4821690.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22512..22766)
                     /locus_tag="LR110_000688"
     CDS             complement(22512..22766)
                     /locus_tag="LR110_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBL4821691.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23166..23435)
                     /locus_tag="LR110_000689"
     CDS             complement(23166..23435)
                     /locus_tag="LR110_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821692.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23905..24360)
                     /locus_tag="LR110_000690"
     CDS             complement(23905..24360)
                     /locus_tag="LR110_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBL4821693.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24498..26426)
                     /locus_tag="LR110_000691"
     CDS             complement(24498..26426)
                     /locus_tag="LR110_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL4821694.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26623..27456)
                     /locus_tag="LR110_000692"
     CDS             complement(26623..27456)
                     /locus_tag="LR110_000692"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBL4821695.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27648..28352)
                     /locus_tag="LR110_000693"
     CDS             complement(27648..28352)
                     /locus_tag="LR110_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL4821696.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28375..29001)
                     /locus_tag="LR110_000694"
     CDS             complement(28375..29001)
                     /locus_tag="LR110_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL4821697.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29327..31702
                     /locus_tag="LR110_000695"
     CDS             29327..31702
                     /locus_tag="LR110_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBL4821698.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31726..32217)
                     /locus_tag="LR110_000696"
     CDS             complement(31726..32217)
                     /locus_tag="LR110_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBL4821699.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32404..33324
                     /locus_tag="LR110_000697"
     CDS             32404..33324
                     /locus_tag="LR110_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL4821700.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33478..33822
                     /locus_tag="LR110_000698"
     CDS             33478..33822
                     /locus_tag="LR110_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBL4821701.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34012..34512)
                     /locus_tag="LR110_000699"
     CDS             complement(34012..34512)
                     /locus_tag="LR110_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4821702.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35040..35123
                     /locus_tag="LR110_000700"
     tRNA            35040..35123
                     /locus_tag="LR110_000700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35074..35076,aa:Leu,seq:cag)
BASE COUNT        11776 a         6169 c         7295 g        10197 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttg
     7861 ctccttatga aaatcaaata tgttataatt aaggaattag aatgggaggt aagcacagat
     7921 ggattctttg tcgaaaaaac ttgcgatcca agtattaaat gatgaagcag aacggatcaa
     7981 acgattaatc cgaaatcaaa acaacagtct ctgtatctca caatgcaaag ccttcgaaga
     8041 agtggtagat acacagatgt acggcttttc ccggcaagtc ggttttgctg tccgtatagg
     8101 agtaatcgga aagaacgagg gccagctgat gttaagtgag ctagaacgag aattgaatca
     8161 gttatatagt gaggtttacc aagaaattta tgataaaaaa gaagttggca aggaggaata
     8221 gacttgccta agaaagtgaa aaaaaggcat ctattagact atagcatctt gattccctat
     8281 ttaattttat gtgtattggg attgatcatg gtatatagtt ctacgtcgta tctattatta
     8341 gaaaacggac aaaatccgtc cgcatcagtc attaaccaga gtattttttg ggtgcttagc
     8401 ttgattgcta tcgcactact ttataaaatg aaaacagatg ttttgaaaaa ccagcgtttg
     8461 atcatggcag ccatcgcagt tttgacgatt ttgctgctga ttgttgtgtt ttttggtaag
     8521 gagatcaatg gggcgaaagg ttggctgcaa attgcaggat tctcgatcca acctgccgaa
     8581 tacctaaaaa tcatttctat ctggtacctg tctctgactt tatctaaaag acaaaacagt
     8641 gtacaaaaag attttcttgg gactgtgaaa cgtccacttg caatggttat cggacttact
     8701 gcattagtcg ctattcttcc cgattttggt aatgcggcag ttatcttttt gattattttg
     8761 gttctgcttt tagccagcgg agtcaattat gtgtatactt tgattgtagg agtaggaggc
     8821 ttttgtctaa gtacttttac gatctggctg atcaatatca caaacggaaa gatcttaccc
     8881 ggacgtttac agtatatcta taatcgattt gctatttacc aaaatccttt ctcagacgaa
     8941 ttaaataaag ggcatcaact agtcaacggc tactatgcca tgttcaacgg aggactattt
     9001 ggcaggggat taggtaacag tatccagaaa aaaggatttt tacaagaagc acaaaccgat
     9061 tttatttatg caatcgtagt ggaagaactg ggagtgatta tggggatttt gatattagcg
     9121 ttgctattct ttatgattgt gcgcattgtc cttgtaggta tccgttcaaa agatccgttc
     9181 aattcgcttt tgtgcattgg gatcggcgca atgtttttga tccaagtatt tgtcaatctt
     9241 ggaggaatta ctggcgttat tcctttgaca gggatcacgt tcccattttt aagtcaagga
     9301 ggctccagtt tgctgatgct ttcgatttgt gtcggctttg tcctcaacat cagtgcggat
     9361 gaaaaacgca agagcttagg tttataataa aaaaagatgt aggagtggtt cgatgaaaaa
     9421 agttttagtt gctaacaggg gtgaaatcgc tgtacgtgta tttcgtgctt gtacagaatt
     9481 aggaattaaa actgttggaa tctatgcaga agaagatgaa tattccgtac accgtttcaa
     9541 agcggatgaa gcatatttag taggacaagg aaaaaaaccg attgatgcgt atctagatat
     9601 tgaagggatc atttctatcg cgaaagaatg cggagctgac gctattcatc cgggttacgg
     9661 tcttttgtcg gaaaacttga attttgcgca acggtgcgaa gaagaaggca tcatttttgt
     9721 tggacccaaa ctgcatcatt tagatatttt tggagataaa atcaaagcaa aagctgcagc
     9781 tatcgaagcg gggattgctt cgattccagg cacagatgga ccaatcgctt ctatagacga
     9841 tgccttggag tttgcgaaac aatacggtta tccgattatg atcaaggcag ctctaggcgg
     9901 cggcggacgc ggcatgcgtg tagctcacga tgaaaagagt gcaagagagg gctacgaacg
     9961 tgcgaaaagc gaagcaaaag cagcctttgg tagtgatgaa gtgtatgttg aaaaatacat
    10021 cgctaatcct aagcatatcg aagtacagat tctcggggat acgcatggaa acgtcatcca
    10081 tctttttgaa agagattgtt ctgttcagcg ccgtcaccaa aaagtagtag aagtagcgcc
    10141 ttgtgtctct atgaatgaac agcaacgtca aaaaatctgc caggcagctg tccaattgat
    10201 gaaatatgtc gggtatgtca acgcaggtac agtagaattt cttgttgaag gagatgattt
    10261 ttactttatc gaagtcaacc ctcgcgtcca agtggaacat acgatcacag aaatgattac
    10321 agatattgat atcgtaacaa cgcaactgtt gattgctcaa ggtttggatc tgcataaaga
    10381 aatcggattg cctcagcaag aaggaattaa attaaatggt tcagccattc aatgccgaat
    10441 cactacagaa gacccgttga ataacttttt accagacaca gggaaaatcg atacgtatcg
    10501 ttcacctggt ggttttggcg tacgtctgga tgtcggaaat gcttacgcgg gctatgtagt
    10561 gacaccgtat tttgattcat tgctagtaaa agtatgtacg catggtgcta cctttgaaac
    10621 agccattcag aaaatggaac gttgcttgaa agaatttaga atccgtggag tgaaaacaaa
    10681 cattccattt atgctcaatg tcatcacaca tcctgaattc caatcaggaa atgcaaaaac
    10741 aaccttcatt gatagtacag caacattatt tgaatttcct cgactacgag accgcggaaa
    10801 caaaacgatg aaatatatcg gtgaaattac tgtgaatggt tttccaggta tcgaaagtgg
    10861 cgaaaaacca ttttatgaag aaccacgtat gcctaaagat ctaatcacaa gaactgatta
    10921 cgtaacggct aagaatgtat tagatgcgaa gggtgccgat gctttggtcg aatggatcaa
    10981 aggacaggaa aatttgttgt tgacggatac gacatttaga gacgcccatc aaagtctgct
    11041 agcaactcgt gttcggacaa aggatttcaa gcagattgca cgtttgactg gagaaggtct
    11101 tcctgaactc ttttcaagtg agatgtgggg gggagcaaca tttgatgtgg cttaccgctt
    11161 tttgaatgaa gatccttggc aacggttgag aaaaattcgt tctttaatgc ctaatacgtt
    11221 gctgcaaatg cttttcagag gttcaaatgc cgttgggtat tctaattatc ctgacaatgt
    11281 attggtggaa tttgtcaaag aagcagcagc gcaaggaatc gatgtatttc gaatctttga
    11341 tagtttgaac tggactccac aaatggaaaa aagtatccaa gctgtacggg atactggtaa
    11401 aatcgcagag gcagctattt gctatactgg cgatatcaac gatccaagtc gggcaaaata
    11461 caatgttcag tattataaag acatggcaaa ggaactagaa caactgggtg cgcatatcat
    11521 tgcaatcaaa gatatggctg ggctgcttaa accgcaggca gcttatcgtt tgattagtga
    11581 attgaaagaa acgacagatc tgccaatcca tctgcatact catgatacca gcgggaatgg
    11641 aatcatcact tattctgctg cttcaaaagc aggagtagac atcgttgacg tggcaatgag
    11701 tgcaatgagt gggaatacta gtcagcctag catgagcagc ttgtattatg cactggtcaa
    11761 tggtccacgg ctgccagaaa tcacgattga aaatgcacag aaattaaacc attattggga
    11821 agatgtgcgc atgtattaca aaccgtttga aaacggcttg aatgctcctg aaacagaagt
    11881 atatatgcat gaaatgccag gaggacagta ttcgaatcta cagcaacagg caaaagctgt
    11941 cggattaggt catcgctggg atgaaatcaa acaaatgtat catactgtca acttgatgtt
    12001 tggcgatatc gttaaagtaa ctccatcatc taaagtagtt ggagatatgg cactatttat
    12061 ggtccaaaat gatcttacag aagaagatat ttatgaaaaa ggcgaaacac taagtttccc
    12121 tgaatctgtt gtgactttct tccaaggaga actagggcaa cccgttggcg gatttcctaa
    12181 aaaattgcaa aagattattc ttaaaggcag acctgcattg aatgaacgtc caggtctttt
    12241 ggctgaatca gtcgacttca acgaagtaaa aaaagaattg gcagaaaaaa ttggctatga
    12301 accaaaacaa gacgaagtat taagctattt gatgtatccg caagtctttt tggactatca
    12361 aaaagcatat aatcaatttg gcgatgttac attactggac acaccaacat ttttccaagg
    12421 tatacgtttg ggtgaaacaa tcaatgtaca aatcgaacgc ggaaaaacgc ttatcattcg
    12481 tttagatgaa atcggagaac ccgatatcga agggaatcgc gtactgttct tcaatttgaa
    12541 tggacaaaga cgtgaaatca ctatcaaaga taattcgatc atcagtacgg tccaaacccg
    12601 attgaaagct gaaccgacaa accgagaaca aattggagca acgatgtctg gttctgtttt
    12661 ggatgtatta gtgaaaaaag gcgataacgt taaaaaaggt gatacgttga tgattactga
    12721 agcgatgaaa atggaaacgg ccatcgaagc acgttttgac ggagaagtcg cacatgtata
    12781 tgtgtcttca ggagacacta tttcgtctgg tgaccttttg attgaagtga ctgagaaata
    12841 ataagcgaaa ggtggggaaa catgaaaaga ctgggtttgt tttggctgat tctgctatta
    12901 gttattgtgg cattctacat ggaaccagtc ttgtttccgc caaaacttga agtgaaggaa
    12961 aactctgatc aacaaatatc aaaccaaatg acaacgtgga aataccagga gcttaaagca
    13021 actggttttt ctacttatat cggaaaaccg atcgatcagc tggaaaagga ttttggccaa
    13081 gcctatgatc gattaactag cggatttggt tttgaaacaa gatattatga aaataaagct
    13141 agtcatctca catttgaagc gaacatccaa gacagcagag tcacaactgt taaagtactg
    13201 caaacagggc ctgctgatat cgcgccgttt tatttaggga tgacgatgca agatcttacg
    13261 aaaatcacta ctatctatac taatttcact tttgagtacc atgatacaga agttggaatc
    13321 gaactaatgg aagaggatat gaattatcgt ccgctaattg cttttgataa cgacactttt
    13381 gctattttat tttttgatca aacaacaaac ggcctatttt cagttgtgta tttagataaa
    13441 gatagtcttt tgaaactatt gccatatcaa gtgtttggtg aaggccttcc acattatcaa
    13501 gcagataaat cggctgattg ggaatcaatc aatcgcgtta aagaacgaaa aagtacgact
    13561 ttattaaata tgcttagaag agaagacgaa cttcccatat acaatcaaac acttgccttt
    13621 caaaaccaca gtcagctgct actacatgat tttttgacaa ggcctgagga aatattgtca
    13681 gaggaacgta tggctgattg gcaaaaatcg ctaggcagtg ctcaaacgac agtgaaattc
    13741 tcactgacga atgatgaatt ggagaagtta atcagtaaac aaaaattaga aaatactagt
    13801 gggctattta ttcatccggc gattgacgct actttttcgt ttttatattg gtacagcgat
    13861 ccttacaacc atgaccgttt catggacccg ggaacggatt ctgtaggcat tgctttttct
    13921 aaagaaaata tgttagtctt attgcaagaa gaaaacggag aaagtagtga tagcagtgat
    13981 cgttaacgaa gagctgtttc aactggagga tcaatgcctc caagtcgctg aaatgataaa
    14041 aaagagtaaa acgatggctt cttatcttga acataaacag tcaatggaag aatctgcaaa
    14101 agtttgccag ttgaaagaag cgttcttgtc taagaaagaa gcatttgaaa atgtagcggc
    14161 atatggtgag tatgcacctg attatcgtga aaaaaaacga gctgttcgaa aagcaaaaag
    14221 acagcttgat atggatgagc atgttgcagc tttccgatat agtgaaacac agctgcaaag
    14281 tctgttggat caaattgtcc aagtacttgc cgaagaagtt tctaatgaaa tcaaagtaga
    14341 tgcaggtagc ccgttttttg aaacgaaaaa acactttgga tgcggaggta attgtcatgc
    14401 aattagacaa tagtgaattt gaaataacaa aacgacgcgg gctgatcgta tgggtctaca
    14461 gcttaaaaca attaaagaat ctgaaaaagt tcggcttgtt gcattatgtc tcaagaagaa
    14521 tgaaatatgt cgtgctttac ttaaatgaag agacattgga agaaacagaa gaacgaatcc
    14581 gtaaattaca ttttgttcga aacgtagaac gttcttaccg accggatatc gaaatgaatt
    14641 ttgccgaaaa aataggaaac aaaacagtat taaaagacga aggtttcgaa attgaagagc
    14701 taaatacaga aatccgatta gctgaacatc tttaaaaatc tcttatgagc agtttttaaa
    14761 taaaaaataa cttggataac gatcgatttt tgatcattgt ccaagtattt ttttggtata
    14821 ctagtaccag taccatttct aaggagtgag tagatgcgga taatatcagg aaattatggc
    14881 gggcgccgtt taaaaagttt agcaggggcg aacaccagac caactacaga taaagtaaaa
    14941 gaatccatat tcaatatgat tggaccatat tttgatggag agacagtatt ggatctgttt
    15001 gcaggaagcg gtggtttagc tatcgaagca gtatctagag gctgcagtca tgcggtatgc
    15061 gtagacaaaa attaccaagc gctaaaaatc atcaaagaaa acatcgaaat tacgaaagaa
    15121 ccagagaaat ttaccacaat aaaacttgat gcggacaaag caatcgaacg gctagcagcg
    15181 gagaaacaac aattcgacta cttgtttctt gatccgccat atgcaaaaca aaaaataatc
    15241 gatcaaatcc ataaaatgga agaactggga cttttttcta aaaccgcagt tatcgtatgc
    15301 gaaacggata aaacagtcga attgccagaa actgtggctg gattccatca aataagaaaa
    15361 cagatttatg gcataacggc cgtaactatc tatagaaaag agggaaatca atgaaacgtg
    15421 cattattccc aggaagtttt gatcctttta caaaaggtca cttagatacg gtggaacgtg
    15481 cggctaaatt attcgatgag gtagtaattg gtgtttttat caatacttca aagaaaagtt
    15541 tgtttccccc agaagaaaga atgacattga tcacaaaagc tgtttctcat cttcctaatg
    15601 taaaagtcat gcatcaagaa aatcaattga cggtagaaac agcaaaagag attggtgcag
    15661 atgcgttgat cagaggcata cgaagtatca aagactttga atatgaacga gagattgctc
    15721 agatgaatca tcatctatat gatgaattgg aaactgtctt cttattagca aaaccagaat
    15781 atagtcacgt gagttccagt attttaaaag aagtactgca ctttggcgga gatgtgagca
    15841 gctacttacc gccagtgatc aacgaagctt tagctggaaa gcgtgaatca aatgaaagct
    15901 aaacaatggt tgaaaacagt actgattatc atatttggaa tgttgatcat tggatttgta
    15961 gtagttccct tgccgtatta tgtagaatca ccgggagcca cgatcgatct gaaagaacta
    16021 attacggtca atgataagga agataaaggt tctgggtctt tctctcttac ctctgttggt
    16081 atcaggcaag ctacaggttt cttagcttta aaagctaaaa tgaccccttt tgaagaattg
    16141 atcagtgaag aagaattgac tggtggggcg acaaatgaag aatataatca aatgcaacag
    16201 tattatatgg attcttccca aaatgcagca atcgaacaag cgttaaaact tgcagacaag
    16261 ccttataaaa tggaattcaa aggcgtatat gtcatgagtg tagagaaaac atcgaatttc
    16321 tttggcaagc tttcagttgg agatacagta acaaaagtag acgggaaaag ctttcaaagt
    16381 acagaagcat ttatggatta tgtaaaaagc caaaaagttg gacaaacaat cgaaatcagt
    16441 tatttgcata atggtgaaga aaaaacagct tctggtgatc tgatcgagtt accaacagat
    16501 aaaaaggcag gaataggtat cacgcttact gaccataccg agatcgaatc agatacagat
    16561 gttcgtatta attctggctc tatcggtggg ccatcagcgg gattgatgtt cactttagaa
    16621 atctatgaac aagtcactgg taaaaacttg cgacatggta agcaaattgc aggaactgga
    16681 acgatcaaca gtgaaggaga agttggccga atcggaggaa tcgataaaaa agtggcaagt
    16741 gcggataaag ctggcgtaga gattttcttt gcaccagatg atacgatttc agacgatgtt
    16801 aagaaagaat atcctgaaat aaaaaccaac tacgaagaag caaaagctgc cgctaaaaaa
    16861 ttgaattcct caatgaaaat cgtaccagta aaaacagtac aagaagcgct tgactatcta
    16921 gagaaaatga aatgaaacta aataattaaa gaggggttgt gacacgagtt ctgtcacaac
    16981 ccctcttttt cgtcaaaaat cttcacgata aaaataaaaa caaaagaaaa aattgacgta
    17041 attagatgtt gaagggacga ttttgccaga gctcactgtt tcttcaattt taaatgaaag
    17101 gagtaatgaa tgatttttac tggtcattct ttgaaaaaag aacaatgata gaaaaaatca
    17161 gcaagaaaat actagcttca cccaaattga tcatttggat gtctatgata gttcttatct
    17221 tttgtatcgc aagtggtttt ttattatcaa atttgctatt ttatagagaa acagaagaga
    17281 ctgctgcaaa agaaatactt tcttcgttta gttccagtgg agaagatcca tcagctgaag
    17341 gaaacgatgt agaagaggat gctcgtccaa aggtcatgta caccgatatc aaaggaagtg
    17401 tgaaggagcc tggtatctat tccttttctt cagaagaacg ggtctatgat gtactaaagc
    17461 gtgcaggtgg attattagaa gaagcggata gcgatcgaat caacttttct gcgaagatag
    17521 aagatcagca agtcctatat atccctgctg taggtgaaga gctgccagaa catcttgatc
    17581 agttggcatc ctcggaagga aaacagccta cagcagattc cgagcctccc aaaataaata
    17641 tcaatacagc cactccatca gaacttcagc agattcctgg gatcggatca gtcaaagcgc
    17701 aagaaatcat tcgatttaga gaagaaaacg gatccttcca gaaagtggaa gacctgcagg
    17761 agatttctgg tatcggtgaa aagacagttg aaaaactaaa aaacttcgtt acaattaaat
    17821 agaatgattc aaaatcgact ggcacgagca ataaatggac gctaatggat actaagaata
    17881 atccaattct aagagcttgg caggacgagg aggtagtatt tttggatagg aaatagcttg
    17941 attgttcgca aagaaacatt aaaaaaacca atgctgaatg tctatctcca aaataaaata
    18001 tctgggattc atattatgaa tacggctgtc agcggcaata acagtcaagc tctacgagaa
    18061 cgctttgcca aagatgtact tagctataca gcagataaag tatttattct cattgggacg
    18121 aatgatctag cagagcataa acaactttct aaagaaacgt accaaaaaat ctgcagtgga
    18181 tgatagttca gctgaaaaaa gactatagtt ctgatcatgc tttctttatc acgccaccag
    18241 cagtcgatga gacaaaacaa aaatatcgga ataacaagtt gatcaaagaa tatatctctg
    18301 ttctcacaga agtagcaaaa cgccatgatt gtgaagtatc gatttttata cggaatttct
    18361 tgaacaaatg gattcgaaga atttttataa tgggctaatg gatgacggac ttcatttcgg
    18421 cagtgaaggg taggatcttt tgtcagacag gatgattcgt tatatttaaa gaatattttg
    18481 tttaagcaat gagtactttt taatcaactt gaaaaaacgg aggaatataa aacatgttag
    18541 aaaaatcttc aaaagcatgc gatacgaata aagaaaaagt tactgataag tcacataatg
    18601 aacgcattcc ttgggaccag tactttatgg ctcaggcagt actgttatct ttacgtagta
    18661 cgtgtacacg tttagaagta ggagcgacac tcgttcgaga aaaaaggatc attgctggcg
    18721 gctataatgg agcagtcagt ggagatgtcc actgcataga tgaagggtgt tacgtcgtgg
    18781 atggacactg cttgcgaacg atccatgcag agatgaatgc actcctacag tgtgcaaaac
    18841 ttggtatccc gacggaaggt tcagaaatct atgtcacaca ttttccatgt ttggcctgta
    18901 caaaggcttt gttgcaagct ggtgtgaaag ggatcaatta tctccatgac tatcgaaatg
    18961 atccttatgc ccaagcgttg atcgaacaat taggtgtatc tgtacaccaa gttgctttag
    19021 actcacagca tttttcaaaa cttcaaaatg aattaacagg aaaacatgtc tagcgtttta
    19081 gaaaggcatg ttcgaaatcg gttgattttt ccggcaatta cgtcgatgat tatctgtttg
    19141 ttgtattaca ctgacaattg gggttatcgt ttgcttcttg ccggaatttt tttatggttt
    19201 tcgctaaaag aaggacgttt tatcgcgtac ttgtgtattt tgtgtagcat aacggtatcc
    19261 atttcttata ggctacaaga gtattcattt ttagaggaaa aaacaacatc tgcgcaaatt
    19321 actgtattta cagatacgat tcgtgcaaat ggtaattttt tgacaatgga aggaaggtta
    19381 tcagagaata aacaaaagct ccagctttct tatcaagtaa acacagaagc agaattgaaa
    19441 acatggctta acttccgagg aaagggaata gaattgactg ctgaaggcga gttcatctct
    19501 ccagcaaaaa agcggaatct ttataatttt gaccaagaag cttacttcag gaaccataga
    19561 atttctggac ggttccaaat tcaaaagatt gaacagatca ccatgcaaaa tcactggcga
    19621 aactggcttc agagaaaaag aggatctttt atctcctttg tccaatctga atttccgcaa
    19681 aaaacaagtg tatatattaa ttctttatta tggggctata aagatgcagg attcagagaa
    19741 ccagaagaaa tttttaggga gagtggtttg ctgcatctgt tcagtctatc gggtctacac
    19801 atccaatttt atttagggtg gctgtattat ttatttcgac gtatcggatg gccactacac
    19861 gtcagtatta ttccgttgag tctactaact atctgttatg tttctattgc aggaagttct
    19921 atttctgttt tgcgtgctag tttgctgttt ttattgcgat tgctagtcaa attgctgaat
    19981 aagcattatt ccaccatgga cttatttgca gttgtctttt ggcttctttt actatttgat
    20041 cctcgatttc tttttcatgc gggtggacag cttagttttt tcatgagttt tttgtttatc
    20101 ctgatttctt ttgatggaga ttggttgaaa aaaacggcct ctcacgtcct gtttactatg
    20161 atcacgatgc cgttgattgt atggcatttt tatgagtggc ctattttagg tagtcttttt
    20221 acattactga ttgcaccggt attcaagttt ctttttcttc ctggtgtgtt atttctcaca
    20281 ctcttcaatc gctggctgcc ggaaacttta cttttgttga tagaagaggg attaatctta
    20341 tttgagcgaa taatcgattt ttcttcaggg tcgtctctca ttataggacg tttgcccctt
    20401 gtgctatctg ggatgtatat tttcggatta ctgatttgta tggataggtt gaaagaacaa
    20461 cctgtgaaac acttgtttta tgttggtctt ttttctttta tttttttgac tttgccattt
    20521 ttacgctttt cttcaacgat cgcttttatc gatgtcggtc aaggagacag tatctttttg
    20581 caaagtcctt ttcgaaaaga aacgattttg atcgatactg gaggtcagtt acactttgaa
    20641 agagaaaagt gggcgcaagg tatgatacgt cctcctgctg aaagaaatat cgttccatat
    20701 ctaaaaggtc aggggataag tgtaatcgat aaactcatct tgacacacga cgatacggat
    20761 catgtgggag aattacccac gttatctcag cattttacta tcaaaaaaat ttatatcggt
    20821 tggggcgctg gacgaaatga gccattacaa aggcatttat ctgaagctca aaaaaatggt
    20881 actgagatca ttgaaatcaa acatggggac aggatcagcg gttattatga tctttatgta
    20941 ttgactcctt ttgaaaaggg agagggaaga aatgaagatt cgattggtct atggatggaa
    21001 tataacaata ggcgcttttt atttttagga gatttgaatc aagcaatgga aaaacaattg
    21061 cttttgattt atcctaatct gaaagccgat gtagtcaaat taggtcatca cggtagccgt
    21121 acgtcaagta acacagattt tctttcccat attgaagcaa aacatgggat tatatcctgc
    21181 ggcgtaaata atcgttatgg tcacccgcac agcgaagtat tggagacact agaagaaaat
    21241 cagataaaaa catggcgaac ggatcaacaa ggaatgatca cctatcgctg gcatccagtt
    21301 tttcatcctc atgggctagt ggaaacaatg atagactagt aattctcatc tgctcatggt
    21361 aggataattt ttgaaagtgg tgagtaattt gagtatacaa gcagagttac aaaaaatacg
    21421 taaagaaaaa cttgcatctt gttacttgat tttaggaaca gaaaagtttc tccaagatca
    21481 agttcggaca gaaattgtca aaaaaatcca attagagggc gaagatgact taaatttttt
    21541 aagttttgac atggaaaatg caagtttaga tgaggtagta gctgaagcag agacgttgcc
    21601 gtttttcgga gatcaacggt tagtattcgt agagaatcct tattttttaa caggtgaaaa
    21661 agtaaacaat ggaatcgaac aaaatactga tttattgacg gcttatttga aagaaccatt
    21721 agaatcgact gtattagtat tctttgcgcc atatgaaaag ttggatgaac ggaaaaaagt
    21781 aacgaaacaa ttgaaaaaaa cagcaattat catcaatgtc caacagttga atgaaaaaga
    21841 agtacgtcaa tatctgatga ataccttaga gaattcctca atcaaaatgg aacgaagtgc
    21901 aattgattta ttcttgaggt tgaccgactt agatttatcc aaaatgatga gagagttaca
    21961 gaaattggtc ctttatggac aaaatcaaca gcaaatcacg gtaagagaag tagaagaatt
    22021 agttcctaaa acgttagaac ataatatttt tgacatgacc caatatattt taaaagggaa
    22081 gacggaacaa gctttgcgtt tatacgaaga tttagttatg caaggggaag aaacaatcaa
    22141 aattaatgcg attttgcttt ctcaattacg tttattttta cagacaaaat ttcttgtaaa
    22201 gattggttat cagcaggcaa atattgctga aacattgaaa atccatccat atcgtgtaaa
    22261 acttgccatg caggaagtaa aaaaattcga tgaacatttg ctggtacagt tgtttgatca
    22321 attagttgag atggattatc aaatcaaaac gggacaaatc gaaaaagaat taagttttca
    22381 actattcgtt ttgcgcacag ggcagttgtt aagaaatcga taagaagaaa gacgagggca
    22441 aagcatgcgg gaaggtcgtt tgtttttgtc ctttttttga caaataaaaa aatcggccga
    22501 agccgattct tttatttagc gatttttttg cttaaacgag atttgtcgcg gttagctttg
    22561 tttttgtgga ttagaccttt tgattcagcc atatcaatag ctttaacagc ttctttatat
    22621 aacgcatcta cgttgtcagc acctgaagct acagcatctt caaatttttt gattgcagta
    22681 cgcatagcgc ttgtttgaga tgaatttttc acattagcgt tttcgcttgt acgaacacgt
    22741 ttgattgcag attcaatgtt tggcattagt ttcacctccg ataacttaag ttcttacgcg
    22801 tgttaccgca taattcaaca ttcattatta tacctaaagg cagcgtgctt tgcaatatga
    22861 acatgacaag tttttttcct taacagtctt ttattaggat tttttttcag taaaaacgga
    22921 tagtacaagc tgaaaaaaat caaaaagacg ctattgtggg aggcaatagc gtcaaaccaa
    22981 actagatttt tatttatcta tttaaacaaa gtttgaatgg gaatgtttgg tactaagtta
    23041 ttatataaca aagagaaccg tttgcaaagt tttttttgaa aagggatacg attccctgtt
    23101 gttctttgtt tttctattaa gaaactgcag ttattttata accagtatcc tttgatcagt
    23161 aaaggctatt ctggtgtacc gattatttta atgtattcat cgatattttt tatcaacatt
    23221 ttttttatga ccaactggtt ccgacggttc catttgccac ttacttccag cacatattta
    23281 tttaggggca aacgtaagat atattttgcc aaatagcgat ctttaagcag gccattgatc
    23341 acacggtcct caagctgcaa gttgatccca gtcagcatca ttggctgttc ttgtaaaacg
    23401 tacgtatcaa ttaaaagacc ttcaaaagtt tccacaagcg tcttcctttc ttttcaaagt
    23461 aacctgaaaa caggcactaa ttcttccatt ttgatattcg ttgaagtgga tgcgatcatt
    23521 ctttcttttg tccactgtaa tctagtataa caaaaaagag cagaagagcc aaaaagaaaa
    23581 aagagcatcg aattctatag gaagtctcat tggcacggcc taaattatta caaaacattg
    23641 attaaaaaaa gcccctgcat cgcaggagca tcccctatat cgttttaata ggtcaatcaa
    23701 aaagcagtat caaacacatt ttgtatcaaa tcaggaaata atagataagc taagcccaga
    23761 atcaaaagta ccgctagaag aaaccagccg attgttccta tgaaatactt taatttttcg
    23821 aacattgtat cacctcgttg tcagtatatc acggatcagt aaattggggg tgttaagaat
    23881 ctcctaaaat agacaaaatt tgttctattt tcctaaagca aatacttgta agatacagta
    23941 agtgatacca gaagcaatga ccggtccggc ggcgattcct cttaagaaga caacacctaa
    24001 aatcgtgccg aaaacaagtg caacagtgat ttgtggatca gaagcaatca atcctacacc
    24061 cttggaagat aagacagcca ctagcccacc gcaaaaaaca gctacccagc caatagggga
    24121 tttaaaggct tctaatagat cacgtagacc gatttctcct gttgcgatag ggattagaat
    24181 agccgctgtg atgatcgtca ccccaatatt gattcctttg ccttgaagcc aagggaaaaa
    24241 ggaacgggag gctggtatga gcttcataat caaaacaaaa gctgccgcta tcatcagact
    24301 ttgattttta gctaaaattg ctacaagtaa aataattcct aaaaaaagcc aactttccat
    24361 gaaatatatt cctcactttc ttcgaaacaa tcataacatg tttttagaaa gttacaaaag
    24421 taggaattca tccttcaaga cctattcata agctgttttt ttcataaaat aaacaaaatc
    24481 ataaaaaaac actttgctta tttgacagaa gacagccgcg ctttgtatag tggacaagac
    24541 ttacattttt ttgaagaatc agaaagatca ggtaaaggtc ttagtaaacg caagccattc
    24601 agaataacca atattgtgct tccttcgtgt cctacaactc caagaggtag atttaaaaat
    24661 tgcaggaagt tagaagcaat caacaagaga ataacaagaa tcgaaaaaat aatattttgt
    24721 ttgatgatcc gccgcatttt taaagatagc cgatgactgt agacaaattt atttaagtcg
    24781 cttttcatta agacgatgtc tgcgatatcc atagcggcat ctgtcccttg ccccatagcg
    24841 acacctaaag aagcggctgc taaagcaggc gcatcattga ctccatcacc aaccatcgca
    24901 tttacttggt aaagttcatt ctcttttttg actagttgtg ttttttcttc cggagtacag
    24961 cttgcatagt aatgttcaat ccctacttgt tcagcaatgg cctttgctgt gccagaatgg
    25021 tcgccggtta tcatacttgt atggatgttt tgggatttaa agtaggaaat tacttgttgg
    25081 gtatttgctt ttggaatatc tagcagacct aaaacagcaa ttaactgctg atctttagaa
    25141 agataaatca cggttttgcc ttgattttcc aaacgttcga tcatttccgc aatctctttg
    25201 ctgattatca tcgtctcagg atcatatgcg tgcttgccaa ctttccattg cgcacctaaa
    25261 taggtagttt gtaatccaaa accagtaatt tcttctattg gtaagtattg gatttcttca
    25321 ggtattgtgc aatcgaaata ttgcaaaatc gcatgagcaa gtggatgcgt cgttttttgt
    25381 tccatagcaa caagaagttg ttgtgccaaa tccttttcat caaagaaaaa gctgtcagtt
    25441 acaactggtt ttcctttagt gagcgttcct gttttatcaa aggcaatcgc ttttaattca
    25501 gaaagctttt ctaaatggat accgcctttg attaaaatcc cattttttgc agaatttgaa
    25561 agagcagcta gagaagctgg tgtagcagat gcgacaagcg cacaaggaga agccacaact
    25621 aataatacca ttcctcgata aaagctttca tcccaactcc aacctaggaa aaagtggggg
    25681 agaaggatca ttagaggaac agcaagcaaa atcaatttta cataattatt ttcgattcgt
    25741 tcgataaatg aagctgtttg agttggcgta ttctgcgctt cttctactaa ttggataatc
    25801 ttagcaaaga cagtttcgga actcgttttt gttactcgaa tcgtgatggg atttccttga
    25861 ttcagcgttc ctgcatacat ctcatcgcct aaatattttt ctacaggcac agattcaccg
    25921 cttaatgcag actcatcgat cgttgcctgt gtgctttcta aaaagccatc tatcggaaca
    25981 gctgctcctt tagcgacaaa taccaagtta tccaccttta gttgagaaac atctacttct
    26041 tgcatttgtc cattttcttt taataattgg gctttttgtg gttgaagatt catcaaagca
    26101 gtaatttctt tttgactttt attagttgtg tattcctcta atgcaccgct taaacaaaag
    26161 atgaatgtca acattgctcc ttcaaagtaa ttaccgatca aacaggcacc aatagcagct
    26221 aaagccatca ataaatccac attcagtgtc cgatcattcc acaattcttt caagccttcc
    26281 gatgtttgtt tatatccacc gatgataatg gcactgatat aaaaaagtgg ggtgatggta
    26341 agagagaatc ggtcgaagat aaaaccgaag atcataaata aaaggcagcc gattgttgct
    26401 gcaaaagctt ttttctgatt agtcatcgtt attagacctc cttgtcaaaa caaggatagc
    26461 ataataaaaa tgtaattgat aataataaca gctaaatgag aacgataatg gttatcattt
    26521 ggtgattaag cttgatgttg tttttattat ttataatgat tattattaaa gaaaataaaa
    26581 aaccagaaca tttctcaaat aagagaagtg ttctggtttt ttctacattg caataaacca
    26641 ataaccaatc aaaatgatac caaggataat acgataccag ccaaaagcgg taaagtcgtt
    26701 tttcttcaaa tagcttagta agaatttgat agctaagacg gaaaccacaa aagatacgaa
    26761 tgttcctaca agaagaacaa cagtttcttc cattttgaat gaattccctt cagctaaata
    26821 tttgacgatc ttcaagccgc ttgcgccaaa catgacagga atccctaaga aaaatgaaaa
    26881 ctctgtagcg acaaaacgcg aagcaccaat caagatagat cctaggatcg ttgcgccaga
    26941 acgggatgtt ccgggaacaa gtgccaagac ttggaataac ccaacaagaa gagctgcttg
    27001 gtacgtaaag tcattcagag aagtccaacg aggtgttttg tttttgttgc gtttttcgac
    27061 aataataaag gcaataccgt aaacgatcaa aacaatggca actggtaaga acttgtggaa
    27121 atttgcttct aaccaatcat ccaaaggcaa accaatgatg atggctggta tgcaagcaac
    27181 gatcacttta aaccaaagtg tccacgtatc ttttttttct accgttgatt tacttggaga
    27241 aaatgggttt aacttgtgaa aataaagaac gacgacagcc aagatcgccc ctaattggat
    27301 aacgacattg aacatcgaca taaattgtga actggcatcc agtttgatga attcatctgc
    27361 taggatcaaa tgaccagtac tactgatggg cagccattcc gttattcctt caataatacc
    27421 gagaatcact gctttgataa tatttgcaaa aaacataaat tccttccttc ccaattaaga
    27481 tacatcttat agtataccgt tattttttga gaaacccaat ctgtttttta atattaagaa
    27541 aaacttgaga aataaagcag ataagtacac gaagagaaaa aagaagacaa agccctcgtt
    27601 cagactttgc cttttatgat tctctggtaa aaattaaaat atccccatta tagaaacgag
    27661 tcgcgactat gtgttttccc gttatcgata taagacgtat cattgatgcc gtggatagaa
    27721 aatcgaccgt ttgcataagt gacctttgtt acgctgccat ttaaaagccc agcacgaaca
    27781 ggctgtttag gatcgatcag attaagcaaa aaggcgatcg tcaaaccatg agatacgact
    27841 aaaacatttc caccaccatt tttttcgcag tgatgggcga tatcttcaaa tcctgtccat
    27901 acacggtctc gcaatgtttc ataagtttcc gcccacccag cggtatctaa gttgtataca
    27961 gcattagcaa tttgttcaaa agaaacttct gtggtaaaca tttcatcata tgttttaaaa
    28021 ttcaatacac gagggatcac accccacatt tccatgtcat atccaccttc aagagacccg
    28081 aaacaccatt ctctaatcct tttttcaata aaataaggaa tttcttttcc cttagaatgt
    28141 tctcctagaa taatacgggc agtttcgatt gcacgaccac tgtcactaga ataagcaaat
    28201 gaaaagtcga cattttttaa cccgcgacct aaataatgaa tgccttcttg tccttgcttt
    28261 gtgaggggtg tatcacacca gccttgtact cgttcaattg tattgaacat cgtttttccg
    28321 tgtcgtacga tataaagtgt agtttctgtc attttattct cctcctaagt cgttttaacg
    28381 attgaaaaaa ggatttgtcc ttttttcctg ttcaatcgtc gttgcatcgc catgtcctgg
    28441 atagactgca aattcatcag gtaatacaaa cagctgggtc cgaatactat gaagtaattg
    28501 ttctaaattc cctgtataca gatcggttct accgatactt cctctaaaca gggcgtcgcc
    28561 agaaacgaca aaatcatcaa aaataaagct gacgcttcct atagaatgcc ctggggtagg
    28621 gacgacgcga aaagacatgt ttcctaaagt ataagttttc atttcaaatt catattctgc
    28681 aggcgagaca ataacatcag caatatcatc atgtctaccg agtccagaaa gattcaaaat
    28741 cggatcacct agccattttt gctctagtgg gctgacataa actgggattt gataatacgc
    28801 gcgtaaaggt tccactgcac cgatatgatc gtagtgagta tgggtaagca aaatcgctac
    28861 cggctgttgg tttgtttttt tgatttcttg tttgattcgt tccgcatctt ctccagggtc
    28921 aatgatcaaa agtgcttctt cgttgtagac caaataacag ttttcttcaa tcattcctgt
    28981 ttttaatctt tcgatatgca tgtgcattcc tccttgatag ccaaaacttc tttcatccat
    29041 tagtataacg cattcattgc taggaaacaa aaacaggtac ttatttctct ttacttatct
    29101 gaattatcag actatatgaa aagtatttgg atattctaaa gagaaggaaa gtaagttttc
    29161 atagcatttt aatatgtcct tgttacatta gattcatcca aaaatccaaa ttctatatcc
    29221 tatatccctg cctaatcagc agggtgtttt tttatgtttg gaaagagagc tctttttgct
    29281 aaactaggtt ttttcaggta atcttagaga gaaagaggtg gtggaaatga tcaaacagtc
    29341 aaaaatatct gtgcggcact tagtagaatt tattttgcga cgtggaagta tcgacaatcg
    29401 taaaaaaagt aaccatactg cattagaagg agcgagaatc caccgcaagc tccaaaaaga
    29461 tgcgggagaa aactatgaaa aagaagtctt tttaaaaaca acagttcaat tagatgaata
    29521 ttacctgatc gttgaaggcc gtgcagacgg tattttcaaa agagacggta tttattatat
    29581 agatgaaatc aaaacctccg aaccacgatt tgaagattta gaaccagaac aagtggaact
    29641 tttctttcat caagcaaggg tgtatgccta tatctatagt caagaacatg atttacaaga
    29701 aataaatctg caattgactt acttccagac ggcagaagaa gtgatcacga gaaaggttga
    29761 acatcagaca agagagcagt tagaaacttt ttttaaaaag ctgactgaag attataaaga
    29821 atggcttgtt tttcaagaaa actggcgcac tgtacgcaat gcctctttga tggctttgaa
    29881 attcccccat gatgagtata ggaaaggtca gcgtgaacta gcggtagctg cttataaaac
    29941 gatccgtacg aaacagaaat tgtttgtaga agcacctacc ggaacaggaa aaacaatctc
    30001 tacgttattc cccgctttga aagctgtagg ggaagaagag ggagaaaaga tcttttatct
    30061 aacggctaaa acaataacta gacaagtagc tgaagatgct atgacagcat taaaagatac
    30121 aggagctgaa gtaaaaagtg tcactttgac ggcgaaagac aagatctgct ttttagatga
    30181 gacgacctgt aatccagatc agtgcccata tgccaatggt tattacaatc ggattaatga
    30241 aggtttatgg gatcttttga atcatgagaa tcagatcact cgtgaagtca tcgaaactta
    30301 tgcaagaaag catacacttt gtcctttcga actatcatta gatgtcagta tctggtgtga
    30361 cgtaatcatt ggggattaca attatttatt tgatccaact gtttatttaa gacgtttttt
    30421 tgaagatgaa aaaaacgagg actatctgtt tttgattgac gaagcacata atttggtgaa
    30481 tcgttcccga gagatgtatt cggcagaact atcttatgaa aaaacgaaac gaagtaagga
    30541 agctatacca aaggaattca aaaaacttca tcgccgtttc aacaaactat taaaagaatt
    30601 tgattcgatc cgagaaatag caaaagaaga tcattgggat tatcatcatc aaaaagcacc
    30661 ggctgaatct ttagtaaagg caggttatca attaagcgaa aaaattaaag aatggctggc
    30721 agaatttcct gagcacccat cacaagaaca attgcttcct tattattttg atttgcttca
    30781 ttttttgaag gtcagtgaat tttatgatga tcactacgaa acaacagtcg aaaaaacgta
    30841 tcgcgatctg atagtaaaag agttttgtat tgatccaagc ttgtttcttg agcagtcgtt
    30901 ggataaagga aaaagcagta ttcttttttc tgctagtttt tcaccactct cttattatca
    30961 ggaaacactg gggggacaaa aaagtctggc ttacaaactg ccgagtcctt tcccagaagg
    31021 aaaccagcaa gttttcatcg caaattacct agagacgact tatcgaaaaa gggaacacag
    31081 tctggcaaaa ttagtagaga cgattcatgc ttttgctgat ggaaaggtcg ggaattattt
    31141 cgttttcttt ccttcttatc aatatttaga tcttgcggtg gaagcattca gacagcaata
    31201 tcccgacagc aatgtgctaa tccaagaaac ggacatgaat gaggaagaac gcgaacgatt
    31261 tttaacaaaa tttcaaacaa atcccgctga aacattgatt ggattttgcg tacttggcgg
    31321 gattttttca gaaggaatcg atttgagagg ggaccgattg atcggcagta tgatcgtagg
    31381 tgtaggtttg ccgcagatga atcatgagca ggaactgatc aaatcatatt atgatgagaa
    31441 ggaacacctt ggatttgctt acgcttatca gcttcctgga atgaacaaag tcttgcaagc
    31501 tggtggtcga gtgattcgtg gcatggcgga tcaaggaatc gttgtattgg cagatcaacg
    31561 attttctagt ttttcttatc gacgtttatt cccacaacat tggcaaacag caagagaagt
    31621 acgaagtgtt ggggaattgg aagaaggaat acagcgtttt tggttatcaa aagaagcagt
    31681 aaatcaaaaa aacgaggaat gacagacgtc agcctcgttt tttgattaac gatagttagt
    31741 gaattgcaac tcgattggta agtcaagttt acgtaataaa gcaatcacag cttgtagatc
    31801 atcacggttc ttacctgtca ctctaatctg atcttcttgg atttgtgctt ttactttcag
    31861 tttttcattt ttgatagctg tagtgatttt cttcgcgttt tcacgatcga ttccgttgac
    31921 caaatcggca gtttgtcgag ctttgccacc tagagcgtgt tctgattcgg aaaaatggat
    31981 atttttgatt ggcacatttc ttttgatcag cttaccaaat aatacatctt tgatctgttc
    32041 gattttgaaa tcatcatcgc tcaatacaac tagattgctg ttctctaatt tgatttctac
    32101 tgtagaatcc ttgaagtcaa agcgattgga caattctttt gttgtttgct gaatcgcatt
    32161 ttttacttct tctgtattga cttctgatac aatgtcgaag ctagcatctt ttgccatagg
    32221 aaccctcctg atatacatat aaatatttac catacatcta tcttatcata ggattagggc
    32281 agattcttac ctgtttgcta aaattcttct catttgagtt cgaagtattt tattttaata
    32341 gataaactgt tattcttaca tttggaatac caaagtaggg gagaggtgct tgtgagtaaa
    32401 attatgactg cgtatcataa aaatgaaaga ttaaagaaaa tagtggttat tttgataacc
    32461 ggattgaccg ctgcggtcgc tttaaattat tttttgattc cagccaatgt cttttctgca
    32521 gggatgaatg ggatcgctca aatcatttcc tcgcttttat atgaatggtt tcatatcaag
    32581 atagacacag gaacatttat ttttcttttg aatatccctg tgtttgtatt agggtttctc
    32641 aaagtaggaa aacgagcaac gatccttagt tttgtgaatg ttgtatgtgt atcggtgatg
    32701 acgatgctgc ttccagtcgg acagatcact gataatattt tgatgaatgc cctagtaggc
    32761 ggtgttttat taggtcttgg cgtaggatta tctttaaaaa tgggctttac aactggtggg
    32821 atggatatca tctctcttgt actttcacaa acaactggaa aaacagtcgg aaactacatg
    32881 ctgattctaa atggtatcat tgtagtcatc gcaggcttct tctttaactg ggaaagtgcc
    32941 ttgtatacga ttatttccat ttatgcaatg actcaagtaa ttgatgccat ccacacgagt
    33001 catcaaaaag taacagcaat gatcgttacc gtcaagccag aagaggtaac agaagaacta
    33061 gcaaaacgga tggtacgtgg aatgacatta ctgccgtctg tcggtggcta ttcaaaaaaa
    33121 caagggaaca tgatcatgat ggtcatcacc cgctacgaaa tgtatgatct tgatcagatc
    33181 gttcatgaaa tcgacgacga tgcatttatc aatattttac caactcattc agtatttggc
    33241 cggtttgcaa atgaaaacga acaaaaaatg tatcgaacaa ctggcgtatt gccagaaata
    33301 aaaaatcgtt cacaaaaaaa atgaggaatc aggtttattt tagaataaat ttaatctatt
    33361 acatctaata agaacatttt tataaattta aacgagaatc ttacttctaa attctcattt
    33421 acaagcagct aaaaaaagag tatgataaat aaaaatcgtg ttaaggagtg tcgtacgatg
    33481 agcgaagcaa accaagaacg gtctggacaa gaaatagaac taatcaaaga acgtattctt
    33541 gctgccttgg aaatggtgat tgatcctgaa ttaggaatcg acatcgtcaa tttaggcttg
    33601 atttatgata tagaattcaa tcctgaaaat ggagaaacag tcattaagat gactctaaca
    33661 actatgggct gtccattagc agatatttta acagaatcta tccatgacgc attaaaagaa
    33721 gtgccagaag taacgaaagc agaagtcaaa ctagtctggt atccagcatg gacgacagat
    33781 aagatgagcc gttatgcacg gattgcgttg gggatacgat agaaatcttg gctgactagc
    33841 tatgagaagc aaataggaaa aacaaaaaag ccttttatcc tttctagaag aaaagagata
    33901 aaaggtttct ttgtttataa aattttgtct gctatattat aagttaatta aataaatgtg
    33961 ttctattgat aaaggacatt caaatccctt tccttttgga atccttcttt tttacatcag
    34021 tttgattttc gtatagtatt ctttctcatc ttcacgataa atcgcagccc catttttttg
    34081 ttgtgcttta agactgccaa gatttttttt atttacagac aaaagtgcct catcaatgcc
    34141 gatttttttt gcctcttgta gtactaatgc taatccttgt gtggcatatc cttttttacg
    34201 ataattaggg gagatgccgt aaccaatatg gccagcgccg tttcttaatg tttcgttcag
    34261 tttatggcgc aaattaaaaa ttcctacata ctcatgttca tcactttcta aaataaagac
    34321 ggttgcggga acgtaactct caggaagatc gattcctaaa gacgtttttt cgcatttttt
    34381 tacataatct aaaaattcag caaatgtata gccataagca ttgttgataa atccactctc
    34441 atttggtcca aagctagttt gtagctgata agctttcttt aaatctgctt cccatagttt
    34501 gatcagtttc atcgtattca cctctttaag taaccttatt catttgtttt ctactatagc
    34561 actattgttg taaatagcca tgcctatttt tatttattgt gtcatatcgt attaagaatc
    34621 atcgaacaat acaaatatga attacaataa aaaaattatt tgaagttcgt tcattcgtta
    34681 cttatgatct ctagaagaaa gaaaacgtga attacttcag cgtctagaat tccagaagga
    34741 tagtaatcaa cagaagaaat aatcaatatc caaatgaaag aagatagctg aatcgagaaa
    34801 aagaggaaaa aagaaatgaa gaaaaaaata aaatagttac taatcccaga gtcgttcgtt
    34861 taacattgaa atcgatcata tggataaaac agattttttc accaaccctc aatagatgat
    34921 ggagttgctc ggatgaatta tttgtgtaga tggcataagg gagggcgtac agaaatgaaa
    34981 aagaacaaaa atatgttctt gacaaaaaac acatgtaatc ctaaactaaa agatgtgatg
    35041 cggctatggc ggaattggca gacgcgctag cttcaggcgc tagtgagggt gacctcgtgg
    35101 aagttcgagt cttcttagcc gcataatttg tttttttata aagagaaggt acacctggag
    35161 cgatgagaaa gaaaataaca tattatctaa aagaatatag gaaaaatttt aaacaacgga
    35221 accagattca aataaatcaa aaaaaatttg tgtgaaggtg ggacagttta aaacctaata
    35281 gattgattca aatagtagat ttatagattc tgatacaaga aatactggca aacgatagtt
    35341 aaggaataaa gactaaactt aaactcaaaa agaggtcatg cgaaagaata aatcgcatga
    35401 cctcttacta tattttgaat gtgtttaaac gttcaaa
//
LOCUS       DAFFSV010000016        34303 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFFSV010000016 DAFFSV010000000
VERSION     DAFFSV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.016"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            154..1380
                     /locus_tag="LR110_000701"
     CDS             154..1380
                     /locus_tag="LR110_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL4821703.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(1469..1861)
                     /gene="rpsI"
                     /locus_tag="LR110_000702"
     CDS             complement(1469..1861)
                     /gene="rpsI"
                     /locus_tag="LR110_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL4821704.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1877..2320)
                     /gene="rplM"
                     /locus_tag="LR110_000703"
     CDS             complement(1877..2320)
                     /gene="rplM"
                     /locus_tag="LR110_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBL4821705.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2839..3306)
                     /locus_tag="LR110_000704"
     CDS             complement(2839..3306)
                     /locus_tag="LR110_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBL4821706.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3426..4964)
                     /locus_tag="LR110_000705"
     CDS             complement(3426..4964)
                     /locus_tag="LR110_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBL4821707.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5044..5730
                     /locus_tag="LR110_000706"
     CDS             5044..5730
                     /locus_tag="LR110_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBL4821708.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5931..6458)
                     /gene="msrA"
                     /locus_tag="LR110_000707"
     CDS             complement(5931..6458)
                     /gene="msrA"
                     /locus_tag="LR110_000707"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL4821709.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6542..7219)
                     /gene="pgmB"
                     /locus_tag="LR110_000708"
     CDS             complement(6542..7219)
                     /gene="pgmB"
                     /locus_tag="LR110_000708"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL4821710.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7216..8346)
                     /locus_tag="LR110_000709"
     CDS             complement(7216..8346)
                     /locus_tag="LR110_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL4821711.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8346..10529)
                     /locus_tag="LR110_000710"
     CDS             complement(8346..10529)
                     /locus_tag="LR110_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL4821712.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10526..11551)
                     /locus_tag="LR110_000711"
     CDS             complement(10526..11551)
                     /locus_tag="LR110_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL4821713.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11596..12423)
                     /locus_tag="LR110_000712"
     CDS             complement(11596..12423)
                     /locus_tag="LR110_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL4821714.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12420..13487)
                     /locus_tag="LR110_000713"
     CDS             complement(12420..13487)
                     /locus_tag="LR110_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBL4821715.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13513..14349)
                     /locus_tag="LR110_000714"
     CDS             complement(13513..14349)
                     /locus_tag="LR110_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4821716.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14346..15215)
                     /locus_tag="LR110_000715"
     CDS             complement(14346..15215)
                     /locus_tag="LR110_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4821717.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15285..16568)
                     /locus_tag="LR110_000716"
     CDS             complement(15285..16568)
                     /locus_tag="LR110_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL4821718.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16582..18267)
                     /locus_tag="LR110_000717"
     CDS             complement(16582..18267)
                     /locus_tag="LR110_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBL4821719.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18542..19567
                     /locus_tag="LR110_000718"
     CDS             18542..19567
                     /locus_tag="LR110_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4821720.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20162..23836)
                     /gene="rpoC"
                     /locus_tag="LR110_000719"
     CDS             complement(20162..23836)
                     /gene="rpoC"
                     /locus_tag="LR110_000719"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBL4821721.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(23894..27511)
                     /gene="rpoB"
                     /locus_tag="LR110_000720"
     CDS             complement(23894..27511)
                     /gene="rpoB"
                     /locus_tag="LR110_000720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL4821722.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28239..29207
                     /locus_tag="LR110_000721"
     CDS             28239..29207
                     /locus_tag="LR110_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4821723.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29225..30130
                     /locus_tag="LR110_000722"
     CDS             29225..30130
                     /locus_tag="LR110_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4821724.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30127..30933
                     /locus_tag="LR110_000723"
     CDS             30127..30933
                     /locus_tag="LR110_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4821725.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31069..32058
                     /locus_tag="LR110_000724"
     CDS             31069..32058
                     /locus_tag="LR110_000724"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBL4821726.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32375..32899)
                     /locus_tag="LR110_000725"
     CDS             complement(32375..32899)
                     /locus_tag="LR110_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBL4821727.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33186..>34303
                     /gene="scm"
                     /locus_tag="LR110_000726"
     CDS             33186..>34303
                     /gene="scm"
                     /locus_tag="LR110_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL4821728.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDT"
BASE COUNT         9781 a         7305 c         6438 g        10779 t
ORIGIN      
        1 tccagtaata aaaattgatg gcgtagttcg ctttgacgta gaggatatca aagatttcat
       61 gaatacattc aaaaaaaatt gaaattttct tctcgtcaca tatactataa atagcgaaat
      121 gtactgcctt actttttaaa ggagtgagga agtttgccta caaagctttc aaatgggaaa
      181 tataaaacta atctgaggta tccaaaaaaa ttcagagaaa tcactggaat tgcatcagag
      241 aagtatcaaa aagtatttcc aactaggcaa ttagctatta aggccgaaag ggaaatgaaa
      301 aagaagattg aaacagtttt aagagaggag aatgcaaact cactagaatt gaaaggaaaa
      361 ataaaattta aagattttta tgaatcaaaa tggcttccta gatatgaact aggtcaaact
      421 actaggtcaa acagggctcc aagtaatgta acaattagta atactaagga tatattccga
      481 ttgcacatcc ttccaatgtt tggtgaatat gcaatgaatt atctgaattc aaatactgag
      541 ataatatcag atgaattaac aaagaaatcg aaagaatacg caaatataaa aattatcaaa
      601 ggctatgttc gctctatgtt tgatattgcc gaaatactat gttatatcga attcaaccga
      661 actaccaaaa ttattcaatg tattaccgcg cctaagaaaa tggctttaga agaaaaaagg
      721 ataagggaag ggaatcaagc attatcatca gaagagctga ctgattggat agaagcagta
      781 aacaatgact taaataatca cttgcttaca cttcacgatt acacactttt catgcttacc
      841 ttatatctag gtgatagaaa aagtgagact tatgctctgc aatggaaata tattgatttt
      901 aataaaaata cggttcgtct caaatatgct ttagataaat atcaaagaaa gacacataca
      961 aaaggttgga aagacaccgt tattcaggca ccagaagttg tgatgacatt attaagagaa
     1021 tggaaatcag ctcaggctga acaattgttg aaactaaaaa ttatacaaac acctgaccag
     1081 tacctcttta cctattccaa accttctgga gaagtaaatt gtcctgttca cgctgactac
     1141 ttaaactatc gaatcaatgc aataaaaaaa aggcatcctg aattggctca attatctcca
     1201 cacaaacttc gtcataccta tgcaactata gctagggaag gtggagcaaa tatgaatcaa
     1261 atctctaatg ctttaacgca ctcggatatt tctactacaa aaatatacgt gaacacacct
     1321 gacgtagttg ataaatctgt ttttgaagca tttcaaaaag gattgaataa gtgtgattaa
     1381 catgtgttct caaatgtgtt cttattataa aaaaagacac tttccaaata attgaaaagt
     1441 gtcctgaaat attgatataa aaagaatatt aacgttttga gaattgtgaa gctttacgag
     1501 cttttttaag acctggtttt ttacgttcga ccatacgtgc gtcacgagta agtaagccag
     1561 cacgtttaag agctaaacgg aagtcaggat caacttctag caatgcacga gcgataccat
     1621 gacggatagc tcctgattgt ccagcgtatc ctccgccgtt tacgttaaca aatacgtcat
     1681 atgcgccttt tgtttcagta acagcgaaag gttgattgat tacttcacgc aagtcagcat
     1741 gtggaatgta ttcttcaaca tcttttttat ttacagtaat tttgccagtt cctggtacta
     1801 gacgtacgcg agcaactgcg tttttacgac ggcctgtgcc gatatattga acttgtgcca
     1861 atgaaattcc ctcctattag attaagtttg tgatgtctaa aacttctggt tgttgtgctg
     1921 catgtggatg ttctgctcca ccgtatacgt tcaattttgt aaattgtttg cggcctaaag
     1981 tgtttttagg aagcatacct tttacagaag tttcgatcaa acgacgagaa tttttagcac
     2041 gcaattcgcc agcagtgatt gatttcaatc ctcctgggta gttgctgtga cggtaataaa
     2101 ttttgtcagt cgctttttta cctgttaatt tcactttgtc tgcattgatg acgatgacga
     2161 aatcgccagt atccaaatga ggtgtgaaag ttggtttatt tttaccacgt agtacagatg
     2221 caacaactgt tgaaagacgt cccaatggaa catcagttgc gtctactaca taccatttac
     2281 gttctacttc gcctggtttg gccatatatg ttgtacgcac gttattttcc tccaatttaa
     2341 gttctgatgt ttgacataca cttcgagttt ccggggctct ttgtggggca aacaatacca
     2401 tttaacatat tacccgctaa tctagtcaat gtcaacgttt ttctgcttga ttgaaaaatt
     2461 tatatgaaac tttacttctt ttctttcttt tatataaaag ggtcgtttcc tgtcaaaatg
     2521 aaaaacaaaa agaggctgtg acaaaagtct tgtcacaacc ccttattttg aataaacggt
     2581 gttctcctgc caactcctcg gtcacaagtc acaatatcca taaaacataa gaagctgttt
     2641 tttaaaattg tttcttgtga ctcggagctg aacggcaggc ttacaacctc ttggtaaacg
     2701 gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
     2761 ttgacgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct ctttttcggt
     2821 caagttcaac aacagttttt acgcgtcgat ctctggcgct ttgcccaaat gagcttggat
     2881 catccagata cgtttttctg tggctgtttt gaattcgata aagatatctt gtgttggatc
     2941 gtcgccttct tctccagaaa cttcgattcc tttttggtaa agatctgcta gataacgata
     3001 gcctgcaaca agtgtttcca aacgttctgg aatagtacga tcccagcggc caatttcatc
     3061 agggattttt gtgttatctg caaattcacg caatgtagaa taaggtgaac cgtctaatgt
     3121 gatcaagcgt tcggatactt catctaactg agcgttcaaa tcatccatga actcatccat
     3181 cattgggtgc aatgtaagga aacctggtcc tctcatgtac caatgtgttt ggtgaacaac
     3241 aacagacatt tggcttagat ctgcaactaa ttgatttaat acttctttgg tttgttggaa
     3301 tttcatcttt cttgcctcct aacattttct tacacttaaa ataatagtag actttcaatg
     3361 aaaagtacaa caaaatcatt tacctgagaa cgattttcag ttcgcttttt tatccgtaaa
     3421 gccaatcatt tttcagaatc agctaattct tctcgcagtt gagcaaattg ctgataagtc
     3481 tgtgctaaat gcttttgtgt cgaaacaaat aatggataga ttttctgata ttttttatga
     3541 tcagtttctt gtactccaac ttgctctttc aattctatca attcctgtgc ttcactgata
     3601 ctttggatct ttccgatgct tctccagcct aaaatcacag ctcctaatgc ggatgcttct
     3661 gtgacagtag ggaaaacaag cgggtaaccc agtacatcag ccatcatctg acgaaaaatc
     3721 ggagattgag caaacccgcc tgtagcacgg atctctgtga gctgattttc ctggcaaact
     3781 ccttttaaaa tccgataaag gttgagacag atcccttcta gaatcgcctt catcatttct
     3841 ttttctgtat gttgccgctg taaaccgata aaactgccaa acgcatctgc gtcccaaagc
     3901 ggtgctcgct caccaagtaa atagggatga aaaatcagac cattggcacc cgctggcaca
     3961 aaagcgatag ccgaaagcag tgcatcatat ggattttctc ctttttcttt agcagattga
     4021 acgacttcct gtaaaaatgt atgccctgcc caatcaaaga tgccagcacc attgctagtc
     4081 gctcctccca ctacccaatg tttctgatcc agaacataac aaaacgtctc catttctgga
     4141 tggaactgag gctgagtagt aataaaacga agtgctccgc tcgttcctac ggtcaaagcg
     4201 gcgatcgtat caccaaaagc acctaaccct agattagaaa gcggcccgtc tgcccctccc
     4261 aaaacaaatg atatatcatt tggcaaacct agttctgctg ctgtttttga tggtagacct
     4321 gttacttgat aggtggaagg aaagacttgc ggcaattgct ctattcgaat gttcaagtag
     4381 tcaagcactt cttctgccca ttggaattca tggatatcaa aatatccagt tcctgaagct
     4441 gttccgtagt cgctgatcag ttcgccaaac aatctataaa agatatagtc tttgataccg
     4501 acaaaataag cagttttttt gaataattca ggttggctgt tttgcagcca cttgattttg
     4561 ttcaatggtg tcattggatg aatcggcgtc ccagtctgcc gataaaattc tttgccaagc
     4621 acggtatatt tcagtttttc tgcttcgtcc gctgctcggt tatctgccca agtgatcatt
     4681 cgagataacg gtgttttctc ttcatccaac aatatcaggc catgcatcgc agaggagaag
     4741 gagacaaatt gaatgattgt gtctgaaggc aactgtttaa ttacttcttt tataacggtg
     4801 cagacacctt gaaagatcaa ttcctgatct tgttctgcca tcccttgtgt ttcttggatc
     4861 aaagggtagc gaaaataagc ggatgctgct tcttgggctt catctgtgaa agcaactgct
     4921 ttggtttgtg tggttccgat atctaatccg attgaaacgt tcattttttc acctcaaatt
     4981 ttatgtatgt acaaaaattt agtttaagct tgcgtatctt ggttttgaaa agggggagat
     5041 ggcgtgttac tttattttat cattggttgc tgtatcggat catttttatg tttaaccgcc
     5101 cagcggattc cgttaggtca ctctattatt tatcctagat ctcattgtgt caaatgctgt
     5161 cgttcgcttt cttggtatga attgattccg attctctcga tcatcgtcca acgatttcgc
     5221 tgccgctatt gcaggtgtcg gttgcccttc tactatttgt tggcagaagc cttatgtggt
     5281 ggtctttttg cctggttctt tactttttct tccgcaagaa atttttcgac gttcatatgg
     5341 atgctttcgg ctttgctatt ttcgttgatg gatttatttt acttcatggt ccattctcac
     5401 acgctattct gttcttgggc aattttatgg attttttggt tgcaggccgg cgtcttccaa
     5461 tggcaaagtg tgctgttaac gtttatcgta ggcggtgtgt gccttcgcta tggtcaatcc
     5521 tatctcggag ctggcgatac tctgttgatg ctttcttgga gtggtggctt gtctctagaa
     5581 gagttgcttc agatcttgtt tcttgctagt ctgtttggaa tatccgtttt ttctgtttac
     5641 tttatctgtc ataaaaaaaa gttggaaaag ctgcctttca ttcctttttt gagcagtgcg
     5701 ttgctgattg tccttcatct taggtattga aaaaaggttg tgacaaacga cttgtcacaa
     5761 cctctgatat atagtgttct cttaccaaat ccttgtgact tggagctagg cgacaggttc
     5821 acaacctctc cactaaatgg tgttcaatca gctgaccctc agtattagct caaaatgctg
     5881 gttttacaac ctctgatata cggtgttcca taagtagctt tttcggcttc ttatttcttg
     5941 acgccccaat gtcgacgaag gaaacctgca cgtccagaac cttctcgata aagattgtaa
     6001 tggagcttgt ttttcttgta ataatcctga tggtaatctt ctgcaggata aaattcgctt
     6061 cttggttcga tttttgtcac gattggctta tcgaatcgcc cgctgtcttg caatgcttgt
     6121 tttgaacgct ctgcaatatt tcgttcttct tcatcattat agtagatcac tggacgataa
     6181 gaatcaccgc gatcagcgaa ctgtcctaat gcgtcagtcg gatcggtttg ctgccaatag
     6241 atttccacca actgatcata agaaataact tctggatcat attcgatctc tactgcttct
     6301 gtatgccctg ttgtgcctgt tgttacttgt tcataagttg gatttggtac atgcccgcca
     6361 gtataaccgg atgtcacaga aatgatccct ggctgctctt caaatggttt gaccatacac
     6421 caaaagcatc cgcctgcgaa aactgctttt tttgtcatat aaaatccccc ttttttctca
     6481 tcataccttg atttccaaaa ggaggataac gttttgcttg tgacagaaaa aacggaaact
     6541 attatatacg tgatacggct tctgataaat gccaaaacga acgtacttga tcaaaaacgg
     6601 ttaaatcagg aatcacatat tcagcagaag gaagatttat gttttcacct attcccaata
     6661 catgcatgtt accagccaaa gctgcttgac aaccagcttc tgaatcttca atgaccaagc
     6721 atgcatttgg cggtacattc aattgctgtg ctcctttgag aaaaacttct ggatcaggtt
     6781 ttgcttttga tacttgcgtg ccgtcaataa ggacatcaaa ataacttgtc aaattcgttt
     6841 tttctaaaat gaggcgtgca tttttgcttg ctgatcccaa tccgatcttg atgtttgttt
     6901 ttttcaagta ctcaagaaca gctattgcac caggcaaaac ggatgtttca tccatttttt
     6961 gaatcgcttg aagatataga ttgttttttt gctcagcaaa ggctgatttc tctttttctg
     7021 agaatttctg ttccattttt ccaaatgaca gcaatcgctc tagtgactcg gttctggata
     7081 ttcctttcag ttgctcgttt tctttctcag tgaatggaat ggacagctgg ttagctaaaa
     7141 ccagccacgc ttcgtaatga taacgagctg tgtctacaag aacgccatct aaatcgaata
     7201 gtgctgcttt gattttcatc gattacctcc ttgaaaatga acgacttgaa gtaaagatgc
     7261 gatgttttta gctgtgcgat gaagattatc agtggcttgc tgtaaagctt cttgtaaagg
     7321 cacagctgca ggaaggatcg gaaaaacggc cgaaatgcca acatctgctg ctttttcgct
     7381 tcctttacca acacttccgc aaatagctat cacaggaacg ccctctggta ctcttctagc
     7441 aatcccaact ggcgcttttc cttttagact ttgtccatct agacgtcctt cgcctacgat
     7501 tacgaggtct gcttgctgta cccgtttatc gaactccagt aaatccaata cgtagtcgat
     7561 gccagagact attttccctg aagcaaatgc taacagtcct gcacccattc caccaccagc
     7621 actactgccc gatgcctcga ttacttgagg ggcaaatctc atataaaaat catgcatggc
     7681 ttggtcagcc actgctaatt cattttcttt caagcctttt tgtggtccga aaacaaaaga
     7741 agcgccttct tttccgcata aaagattagt aacatcagta atgacagtga ttttggcttt
     7801 aatcagctca ataggcacgt tcgttttatc aactgtagca attttaccta gattttctcc
     7861 cagtgcttct actttttgat tatcttgatc gtaaaaagca taacctagac cttctgccat
     7921 gccgatgccg ccatcgttgg tactacttcc gccaacgccg atgatgatgt gtcgaacacc
     7981 ttgttgcaac aattgtcgta tcagttctcc tactcctcgc gtggataacc taagtggctg
     8041 ttgttggttt agtgggacat ctttcaatcc gacgatttct gccatttcaa aaatcgcagt
     8101 ttgaccgtct gaagcgtact ggatttcttt gtttttgcca aatgcattag taacgactgc
     8161 tgtttgccgc ttcaacctta aactgtctgc taatgatgaa accgttcctt ctcctccatc
     8221 gcctataggt agcagatcat attcagcctg aacaaaaact tcttgaaaac ctgattgaat
     8281 ggcttgtgct gcctctaagg ctgtcagact ttctttgaaa gaatctggcg caatcagaat
     8341 acgcattatt ttcccctccg ttccaagatc agttcttttt ccagctggta ttggtctcca
     8401 ttgacaaaaa taggtaatgt ttctttcgtc tcttttttta ttactatttg tttttttgtc
     8461 aaaacaaata gcaacctgtg atttttccaa ataaaaggaa aagaaatttt ttcccatgct
     8521 gaaggaagtc ttggagaaat agaaagagtt ccattcgtta ctgtaatacc tgaaaaacca
     8581 aatataactg ctagccatag tgctcctaat gaagcagcat gaattccgtc atctgatgaa
     8641 tgcggatctg gtcctaaatc aatcaggcac gcttcttgga ataaatgata cgcccattcc
     8701 atttcttgga tacggcaagc ctctatggcg tgaatcgctt tactcaacga tgagtcgtgg
     8761 attgttttgt tttcatagta atgcaaattc ttagaaacga ttggttgaga aaaatactca
     8821 ggtaataagt ataacagcat gacaagatct gcttgcttgc tgacttggag ttcatttact
     8881 tcgctacggg cataatcaag taaaatagac tggctgccag gacgttcttt gtaagtggtc
     8941 aaatcaataa ctggcttact gagatacgtg tcatcttgcg gaatcagctg ttctttgtta
     9001 ggcactggca aatataactt atctaaaaaa ttttgacatt ctttcaaaaa aagcggatcg
     9061 ccagcttgat atttaacagc taaggccaca ttataatgcg ccagatagtt cgtatatgca
     9121 ttattgtcga tatattctgt gtattcatct ggaccaatga ctctgtgtat ctctaaatga
     9181 tcatcgactt ttacagctct gctcagccaa aaagctgctg tttcatgaat gagcgttttt
     9241 ccctttgttt ctaaaaacga ttgatcctct gttgcttgga catactcttg aacggcataa
     9301 gcaaggtctg cactgatatg atgttctgcg gcagctgaag caacttcttg tcttgttccg
     9361 gttttgatat tcaatgcagc aaatttgggt gtctcttcct gaccagtgtg tgcgctttcc
     9421 catgggaaaa gtgcaccttg atagtgataa tctcttgctt tttgccgtgc gccttctaat
     9481 cgatcataac ggtacagcag caaattcttt gctacttcag gaaactgata taaaaagaaa
     9541 ggcaggataa aaatttccgt atcccagaaa acatgccctt tataaccttc gccagttagt
     9601 cccttcgccc ctacactaac acttggatca ttgcggggag tcataatctg taaatggtaa
     9661 gcagcgaagt ccatcgctaa ctgatcgaaa gaagaatcgc cttggatcgt tatccgatgc
     9721 tgatgccaaa aatctgtcca ataagacatc gactgctcta aaatttctgt ataactaacg
     9781 cttttctggg cttgggcaat acttttctta aacgggtctg tttgctgaag agaggtagag
     9841 agcacgatga tcttctccag cgtaaaggac gtattttttc tgatcttttc tgaaaaggaa
     9901 gcggtgatcc ggcgattttt tgcttggaat acacctgctt tattgaattt agctgcaatc
     9961 ccaatatcaa ttttactttc tgttgtctgg taggatgcac tgatcaaatc gtcaccaaat
    10021 actcgcagtt ctttttcaac caactgctgt gtaccaaaat tggtttgctg tccatcgata
    10081 cccgttttca catctacttg gcagtcttcg gtcaacggct tgatgaccac tttcagggct
    10141 gcctgatgga gattattttg atcagcaaca cgacaagtaa tgacttccac tttttttcct
    10201 tgagcagttt caatgagcgc atagcgttta agctctccca tttctaaatc tagtgcccgc
    10261 tcgtatgcaa gaactttcat cgtctcaaga gaaaaatgtt caccgtcgat ttttatttcc
    10321 catctgatga catctgggag attcactaac tcggctgatt ctgctcctaa aggtcggtta
    10381 tatacaccag ctgcaaacat tccccttact tgattccgat agtcttcctc ctggcttcct
    10441 ctgataccaa aaaatccatt tccaatggtc atctgactcg catacttatt gatatactct
    10501 aaatcaaact gtttttcttc tactttcata aggtaataac ttctcctgtt tctgccgaac
    10561 gatataaatt ttccaccaga ttctggatga catatccttg ccagccatct gcaatcatct
    10621 gagtctggtc accgagcgcc cgattcacaa aatgagccat acttagttca tgtgggtcat
    10681 taactggcgt ggtcttttgt aaggaaacca gctttccatc tacatcttcg aatagttcgc
    10741 ctggatacag attagcacct gcctgatcgc cacaaaaagc cacattcagt ttgtcttctc
    10801 tgatattcaa agcaaacgaa gtattcagtt ggatgatctt gccgttttct aattccagtg
    10861 tgccaaaaat tgcatcttct actgtgaatt tgcttggatc ccatttcccg aatttccctg
    10921 aatttttttt cgtaccaatt ttttggaagc tgtacgcccg aacgcttttt actttaggaa
    10981 atccagtaat atacatcgct gtatcaagca tgtgaatgcc ataatcgatc aaaggcccgc
    11041 ctccttgaag ttctttgtcg ataaacgttc cccagcctgg aacaccagac cgacgaagcg
    11101 cattcgcttc tacgtaataa atatccccga gaacatcaag atgattcttg atcattgttg
    11161 cttcgtcaga aaaacgatgc tggaatccat aagccaaaat ctgcccgttt ttatcggctg
    11221 tctctgccat ctttcttgct tgatcagctg tcatagctgg tggtttctcg cacatcactg
    11281 aagcattagc ttcgagggca gccaatacat gttcgaagtg aaagcgattg ggtgtacaga
    11341 cactcacgat atcgggagaa atctcattca gcatttgtgt aactgatgta tacgcatgag
    11401 aaaatcctgt acgtcggcaa aagtcttctg ctcgctggct gactggatca accacagcgg
    11461 ccaattctaa gacatctgat cttttttgat aataagcagc atgtactttt tctgctactt
    11521 gtccagcgcc tatgattgct actttcttca tttatttctc cctcctttgc ttatttactt
    11581 attgttattc tttctctatt ctttctctaa atcatttaaa tgacgcgctg tttccagaca
    11641 gtttttcaag tattgcaatg attcctgata aattttcact tcattttctc ctcgcaaacg
    11701 acattcatat accatataac cttcatactc attttgaagt agcgtttgaa aatggcgagt
    11761 aaaatcaatt gcgccagatc ctggttggta gcgatgatta tctgctaaat ggatatgacc
    11821 cacatactct ttttgcgtag tcaatgcttt tgaaatatca tcttcttcaa tattcatatg
    11881 ataaaaatca gcgatgatct gcacatgttt caattgatta gagtcaatgt aatgacgcgc
    11941 atccgctaac aaattgatca tatggtcttg gtatcggttc aaaggctcca aataaataac
    12001 cgtacctgtt tcttcggcta ctttatctaa ataacgaagt gattcgctga ctgctttata
    12061 gtctccttct ttgcttcggg gtgaaaccat cggcggtaat cgataagtga acattcccca
    12121 agctgctggg ataacgattc ctttgcctcc aacctcgctt agtgcacgca aaatctcagc
    12181 aatttgacgt acaccattaa gacgccgttc ttcgataaag tcaccgatcc acccatcgta
    12241 gccgttacaa gctgtcacta ctgggatccc tgtttctttg attgcctgtt tgactgtgtc
    12301 gatattttct attaacaact taccgtcgat ttcgtattct tcaaatccac agtctttgat
    12361 aaatctaaat ttttctaaaa tgtctcttgg gaaaaatgct tgatcttgcg taccgaattt
    12421 cattgttctc ctcctcggac ttgaacgccc attttgatac tttcttctgg gtgctggtct
    12481 acataacgca tgtaagcttc tgcgctttct gcaaatggca caactggatc aatgatttct
    12541 tcacaatcca agtaaccgtt catcaataat tcccaacatg tgtcctcgat tcgtttacgg
    12601 ttccatcttg ggtaatctgg gtttggttca ctagatgctc gagcaaagac gatttttgca
    12661 ttattgaaat gtgcttcgcg accaagatta aacccagctg gaaaaggttt agcaaaggca
    12721 acataagaaa tgatcccgcc atagccgatc cctcttaaag aagcttgtaa tgcggcagca
    12781 ctgccactcg tttcaataac tgaatctact ccacgtttat ctgtcaattc ctttagacga
    12841 aaaccgacat ctgctttcat aggatctagc gtgtaatcgg caccgtattt ttctgctaat
    12901 tctctacgct gcgctatggg atcgatccca acgatggtag tagcacctgc ttttttagct
    12961 aattgaatcg caatttgccc gatagcgcct aaaccgaaga ttgctacagc atcacctgct
    13021 cgaacatgtg cttcccggat accacctaaa gcgaattgag caggatcgta acatactgca
    13081 tttttccatt gatccaaccg atcaagttta cgtagtttat aattatcaac accgtttgta
    13141 atcagcgttt cacgaatggg tccataagtg cagactaact ctcctttttg atactctgtc
    13201 accaaactgc ccgtttcttc gatcgtaccg acaatcatat ttcctaattg aaattcgcca
    13261 aataccacac cgcgttggct tccttcttct cttggcaaga ataggcgcca ttcttctgaa
    13321 aattcttcat cgataaatgg acttaatcct ctaaagtcga caatttctgt tcctggtttt
    13381 ggagtagcga aaattactcg gatacgtact tcgttctttt cgatctcacg gtcttcgtaa
    13441 tctttgaatc cggctattct tggttctaat gcaactaatt ttttcatctt ttctttcctc
    13501 tttcttttct ttttatcctt ttacgccgcc ttcgactaaa tcacctttaa taaatcgttc
    13561 agataacgca tacataatga tgactggtaa agcggtcacc aatgaagcgg ccatcattcg
    13621 tccccaaatg tagtctggtg tactgaataa tgtgttcaaa ccgattggta aagtaaattt
    13681 ttcggaggtc gacaggaaaa tcgaagcaaa taaataatca ttccacgaaa ccataaagca
    13741 ataaacgaag acagaaatga ttccagaaat agccaatggt ataatgatcc gaataatgat
    13801 ctggatccgg ctaagaccat cgatcattgc tgcttcttca atatcagtcg gaatcgtgtc
    13861 aaaataactt tttagcatga atatcgcagt aggaagtgtt tgaacgacca tcgtgataat
    13921 cagtgctgta ttggtatcat acaaacctag accggaaatg attttaaata atggaacaac
    13981 gatcaaaata ccggaaaaca tataaacggt gtaaaagcta gcattgattt tcgagcgagc
    14041 tttgaagcgt agttttgaca aagcgtatgc cccgctgatc ccaatcacta ctgcagttac
    14101 ggaggctatc aaggagatca ctaaactatt tttgaaatac atgatgaatg gaaaaatctc
    14161 agcattgaaa atatcctgat aatgttcaaa agtccattct ttcggaaaaa tagtttgtgt
    14221 cgttgcaatt gcttcttgtg aatccttgaa tgatgtcatc agcatgatca aaaatggaaa
    14281 caaattgaca gctaatagca acaaaatcgt cgcgtaaaat aagattttcc tgctcttttt
    14341 atttttcatt tcaagtccac cttctttcgt gagagcaaga cgaccgctgc aataatgatg
    14401 aacaatagaa tcgatatagc tgctgctttt ccaagatcat tgaacgcaaa ggctgtctga
    14461 tacaaataaa cgcctaagat attgacttta ttcgtcagta gatagacatc tgtaaacata
    14521 tagaacatcc agatagatcg taaaacaatc acagttgcta gtactggttt gattgccggc
    14581 agtgtcacga tcttgaactt ctgccaagca tttgcaccgt ccatttcagc tgcttcataa
    14641 agcgttttgt cgactgtttg caaaatagcc aaaaatgaaa taaacgcata cggatagtat
    14701 ctccagatag caaacaaaac gaccataaag aagctagcaa ctggctggtc gaaccacagc
    14761 ggtgcttcgt tgaaaagctg caacctatct actaacagat cattgacgat tccataacta
    14821 ttattgaaca tatatttcca agcaaaaatc aaagaaatag atggcgtaac ataacttaat
    14881 aaaatcagtg aacgcaccgt tttcctaaat ctgaacttgc ggttaagcaa aatagctacg
    14941 gctaatccta aagctgtact gcctgctaca actaagaatg tgtaagcaac tgttaaacct
    15001 aaagaacgat aaaattcact atcacttaaa atttcaagat aattgcttaa tccgataaaa
    15061 acggcgtctg agttaggatt cagcggcata tcgaaaaaac tgatctgaat attggaaagc
    15121 attggaatca gcactaaaac agctaataaa agcatgcttg gtgctaaaag tcccatggct
    15181 aattttaagt cagaatgttt ccttggacga ttcattgaaa cctccccctg ataatctgcc
    15241 aatagctatc tacttttttc gaacaccatt ggcagaactt cttgttattt cgtatttttt
    15301 tgtgcgtcag ctaaagcttg atcgacatct tcgtttccga cagttacttg gttcactgct
    15361 tttccgatca cacctgagct tgtgatatct cccattttcg tgaaattctt ttccccgact
    15421 agtccaaaga cttgtacttc atcaaaagca ctagcaatct cagatggcag ctcgccaaaa
    15481 gacttgatga cttcattttc tttgtaacca tccagttcga ctacttgttt attcactggt
    15541 tgagcaccac caggagacat caagacccac ttttccatgt ttttcggttg agataagaaa
    15601 gctgtgaatg cttcagcagc ctctttttgt ttctcgtcta gaccagaaga aatcgtcaat
    15661 ccagatactg tgccatagac agcttgatct ttttgtgttg ggatagcaaa accgatattt
    15721 tctgagttcc ccgcttcata aacagaaggc aaaatgtaag ttgaatagat tgccattggt
    15781 gctgttccat tcatgaaagc atctttgatt tctgtcacat cattagagcc tggcatcgtg
    15841 tattgggata agttttgata aaacgacaat gcttctctca tcttttccgt atcgatcgtt
    15901 acctcgcctt catcatccaa aacatttgct ccatttgaca aagcaaattg agagaacgct
    15961 tgctcactca ttgttccttc agcagtaggc atagcaattc catattgttt attctcttta
    16021 tctgtgaaat gttgagcaat ttttaaaata tcatcccaat tttctggttc tgaaaaacca
    16081 gcttctgata atttttcttt gtcataccaa ataccttgaa cccagccgct aattggtgca
    16141 gcaatatagc tttttccatc ttctgaacga acaaggtttt tggctccgtc ataataatta
    16201 tcttctccag cttcctcgat aacgttttgg actgccgtct gatcaatcag ttcatcttta
    16261 tccatcactt tagcaaaatc ctgactgact tccattactt ctggtaattt accggaacga
    16321 gctaacgtaa cgatttttgt attgaacgct gattcttcta ccggcacttg cttgattttg
    16381 atgtctggat tttctttttc aaaatcagca accagttcat tgatcacact taatcgttcc
    16441 tgttcgacag aagagtgcat gaattgaatc gttgtctgtt gtgattcgtt agagctttga
    16501 ctttcaccac aagcagtaag aagaaatcca gtagctagca gacaggtagt aacaataatt
    16561 tttttcattt ttttccacct cttattttat tattttcagc cacatatatt catacggctg
    16621 gaatatcaac tttcgttctt gactgcaatg gataatttgt ccagaaaaca ggtctaggta
    16681 atctcctttt ggtaaaagat aacctgctga ttgattggaa aaattatgga taactaagac
    16741 tttctcttcc ttcgcttcgc gaataaaact gataaaagaa tctgctgagt ccagtaagta
    16801 aatcaaacta ttcggatgga aggctttttc tttttttcga atagatagca tctgggtcaa
    16861 ttcaccatat actgctgttc ttaatggatc tgtctgcaac tctttctcaa tatctgctaa
    16921 caaaaatttt tgccgattga tttttcgatt ctctcctgtt ttttctacgc ctttgtaatc
    16981 atttcgactt cccagcacac tttgaatata aattgccggc acgcctggta acgttaccaa
    17041 aaatgaatga gcagctaaaa agcgtttgct tctccattcc gtcgtatctg ttggagaact
    17101 taacgcatct agatacgtag cgtttatctc atatggactg gtcgttccat ctgggttttt
    17161 cttgtagttt actcgggtac cctctttctt cagctgttcc actaattcaa ggatcgtttt
    17221 ttccggcaga atacctcgta ccggattcaa tccgataccg tcatgggagg ctaaaaaatt
    17281 aaaaaaagtt gtttttttcg atgcgaaatc gatggttttt aaccattctt tcaacttact
    17341 tgtattaccc gaacgaatcg cttgaagggt caaaggtggt agcgaaaact gatagaccat
    17401 ctgtgcttca ttctcgccat tcccaaaata actgatatta tctttgtgtg gcacatttgt
    17461 ctcagtgatc aataccgttc ctggagctgc ttcatcagcg attgcacgga ataattggat
    17521 gattttatgc gtttctggca ggtgtataca agaagttcct agttctttcc ataaaaatcc
    17581 cacagcgtcc aagcgaataa atgaagcgcc tttttctaag taaaacaaaa gaatatcgat
    17641 cattttcaat aaaacttctg gatttttaaa attcaagtct acttgatctt cgctaaaagt
    17701 cgtccatacg tgtttttctt tcccgttttt gtcaatgaac gttgtaagta acgggctggt
    17761 ccttggtcta gtcacgctgc ttgtatctgt atccagtggc aaatcaataa aaaaatcttt
    17821 gtacgtatca ttcaagttta aatattcctg aaaccaagta ctttcggcag acatatgatt
    17881 gcagacaaag tcgaacatta accgtgtttc gcttctcatt tcttctatat cttcccatgt
    17941 gcccaaacgg tcgttgattg cctgataatt tacaacggag aaaccatcat cagatgtata
    18001 tgggaaaaat ggcaagaaat gtacaactct aaatgtatct gccaaatact tttgataaaa
    18061 tttcttaaat gtacgcaaag ttggttgctg ctcttcaaaa aattgatccc cataagtaat
    18121 caagaaaata tcttcttcgt cccaatgcag tttgcctgcc tggcctttct tctgctgata
    18181 aacactgact ttttgttcaa tcatagaggc ggttttctca gacaaatctg agtcgcgata
    18241 aatcgcttgt aaaagttttg tatacatcgg attccccttt cttttttggg agcgttccca
    18301 acgatttgat catattatgg aaaacgctta ctgtcaatag cattttttta aataattttg
    18361 atatacacaa aaatacaaat aagaaagctt atgtatcaat aattaatagc ttcttaaaaa
    18421 aatatggtct ctcatgatca tgcaagctat tcctaaattt tcaaaatcat agacaaaaaa
    18481 gaatgtctat tgtactatgg aattattcta aaacgctccc aaaaaggagg tgcatataaa
    18541 attgaaacct actatttatg acattgcgaa aaaagctaat gtctcaaaat caactgtctc
    18601 cagagtatta aataaccagc caaatatctc tgataaagca aaagcaagtg tacttcaagc
    18661 aatcaaagaa ttgaattatg aaccgagcaa aattgcaaga ggactgtcag gaaatggttt
    18721 tgacgcgatt ctagcattat ctcatcgtac ttctcatacg acctatggca atcctttttt
    18781 ttcagatgtt attcaatcca tttctgccgt ttctgaacaa aataacttcg acttgattct
    18841 acaaacggca aaaacaccag aagaagagct agaaaaatgc gcgaaaaaga tccaagaaaa
    18901 aatgatccga ggaattatta ttttaagctc tacgatggat gaaacacttc tttttgaatt
    18961 ggatcaatac aatattccta ttgtggtaat cggaaaagtc ggaaacgact acacgaacat
    19021 ctactcagtg gacacggata atttccagga tagttacgat ttggtggaaa cactgatcaa
    19081 gaatggacac cgtcatatcg gctgtctcca tgctccgaca gaagtttatg tatccaatga
    19141 tcgtctcaat ggttaccgtc aatgcttatt tgatcaccac ctagatatcc gtaatgaatg
    19201 gattatagac ggcggctttc gcttagaaga tagcatggca gcaatgaatg atttaatgaa
    19261 gctacgagaa agaccaactg cgattttttc taccgatgca ttaaaatcat tgagtattta
    19321 caaagtggca gaacaatctg gaaagcagat acctgatgat ctttcgatcg caggattcag
    19381 tgatccctta ttgtcctccc tattttcgcc acagttgact cggatagaca ttccgacaaa
    19441 tcagttagga atcaaagcca cagaactttt attccaacta atccaccacc aataccctga
    19501 agaaaaaaga atcattcttt caaccgagat tactctcaca gattcgatcc aacaacaaga
    19561 aatgtagaac agaattataa aagaacagaa acactgagga taagtcagtt actctgttct
    19621 tttttcaatc actcatccct cgttaccccc tcccggtatt ttagcgaatt tttcttattt
    19681 tccatctgtt ttagatagct gtggataact ataatgtttg tgttaaacta ttttttccac
    19741 agggaagaaa aaactatttt taaccacgtt gtgtataagt ttttatagtt cgttttttta
    19801 aacacattta cccttatttc aatccatgtt atttggggat acttatctct aaaacataaa
    19861 tagaataaca gacatgtcta ctggttttgt tttattcgag gaattattca ataatagcta
    19921 gcaaaatatt aaaaataacc gctctatctt cccatcaatt tattttgcat tcaaaaaatg
    19981 atctatcttt acttttgttg attgacttct cttggaagat gatcgttccg ataccttaat
    20041 tggatacgaa ccgatgttgt taagatcctt tcccactttt cttctagtta gacaaattat
    20101 tttagacaaa ccaaaaaaga ggttgtgaca ataatttgtc acaacctctt ttttaaaata
    20161 tctattttgt ttgattgttc attgcgtctt ctgcagccat ttgcgcttca atatctgaaa
    20221 tactgtagac attttcgctt gctacaccga cttcttttgg ttccatgtca cgataacgtt
    20281 gcataccagt accagctggg atgatcttac ctataataac attttctttc aaaccaagaa
    20341 gaggatcttt cttaccgcgg atcgcagcat ccgtcaagac acgagttgtt tcttggaatg
    20401 atgcagcaga taagaaacta tttgtttcta atgatgcttt tgtgatacct agtaagacag
    20461 gtcgagaagt tgcaggaacg cctcctgaga ctagcgtatt gtagttttga tctttaaagt
    20521 ctgcgatgtc taataatgta cctggtaaga tatctgtatc acctggatcc attacacgaa
    20581 ctttacgcag catttgacga accattactt cgatatgctt gtcgccgatt tctacccctt
    20641 gcatacggta aacacgttgt acttcacgaa gcaagtagtt ttcaacagat aatacatcgc
    20701 gaacttgcaa caattgtttt ggatcgattg atccttctgt caatggtgca ccacggtgga
    20761 tcatatcgcc ttctgctact ttcatacgtg ctgtataagg aacagtatac gtacgtgtat
    20821 ctgtttttcc tttgattgtt acttctttgg tacgtgttgc aggatcttct gagatatcga
    20881 tgacatcccc tgttacttct gtgatcactg cttgcccttt cggattacgt gcttcaaaga
    20941 tttcttggac acgaggaaga ccttgagtga tatcgtctcc ggcaacccca cctgtatgga
    21001 atgtacgcat cgtcaactga gtaccaggtt caccgattga ttgagcggcg atcgtaccaa
    21061 ctgcttctcc gacttcaacg tcagagccag ttgcaaggtt gcgtccgtaa caatgtttgc
    21121 agacaccatg ttttgtatta catgtgaaga cagaacggat tgtcactgtt tcgacacctg
    21181 catcaacgat ttgttttgcg atgtcttcgg aaatcaattg gtttcctgca atgatcaatt
    21241 catttgtttc aggatgacgt acttctttac gggtgtaacg tcccactaaa cgatcttcta
    21301 atgattcgat gatttcattt ccctcgcgga tggcttggat ttcaagacct cggtcagtac
    21361 cgcaatcttc ttcacggatg attacatctt gggccacgtc aaccagacga cgagtcaagt
    21421 aacctgaatc ggcagtcttc aaggctgtat cggtcatccc tttacgagcc ccgtgagttg
    21481 agataaacat ttccaagacg gatagtcctt cacggaagtt cgaaatgatt ggcaattcca
    21541 tgattcgacc attaggagcg gccatcaaac cacgcattcc ggcaagctgt gtaaagttgg
    21601 aaatgttacc acgagctcca gaatcagaca tcatgaagat tggatttttc gcttccaaac
    21661 cttccatcag tttttgttgg atgctgtctt ttgcagcatt ccaaacagca atgacacgtt
    21721 cataacgttc gtcatctgtg atcagaccac gacggaattg ttttgtaata gtttcaactt
    21781 gtttatgggc attttcaatg atttcttgtt tttcattcag tacactgatg tcagcgatcc
    21841 ctactgtgat accggcatgt gtcgaatgtt tgtaccctaa gtctttcata cggtcaagca
    21901 tcttagaagt ttctgttact ttgaatcgtt tgaagacttc tgcgatgatg tttcctaagt
    21961 ttttcttctt gaacggtaat actaattctt gctctttgat atgtgcagga atatcagtac
    22021 cagcttcaac aaagtatttg tccggtgttt ggacggtcaa gttgaagtct gttggttcat
    22081 tcagataagg gaattcagga ggcatgatct cgttgaagat gatcttacca actgttgtga
    22141 tcatcgtacg ttctttttgc cattcagtaa atggtttttc acctaatgag ttagggttca
    22201 caccgatacg tgaatgtaaa tgaacatatc cgttgcgcca tgcgataacg gcttcgtttt
    22261 gatctctgaa gaccattcct tcgccttcgc gtccttcttc ttccattgtc aagtagtagt
    22321 tacctagaac catatcttgg gaaggagtaa caactggttt accatcttta gggttcaaga
    22381 tgttttgagc agctaacatc aacatacgtg cttctgcttg tgcttcttca ttcaatggaa
    22441 cgtggacagc catttggtct ccatcgaaat cggcattata agcttcacat acaagtggat
    22501 gcaaacggat tgcgcgtcct tgtactaaaa ctggttcgaa tgcttggata ccaagtcggt
    22561 gaagcgtagg tgcccggttc agcaatactg ggtgttcctt gattacgtct tctaaaacat
    22621 cccatacttc gtcttcttga cgttcgattt tacgtttcgc atttttgatg tttgaagcta
    22681 gttcgcgttg aacaagctca cgcatcacga atggtttgaa taattcaatg gccatttctt
    22741 ttggaagtcc acattggtac atcttcaaga aaggtccaac aacgataacg gaacgacctg
    22801 agtagtcgac acgtttacct agcaagtttt gacggaaacg accttgtttc cctttcaaca
    22861 tatgagaaag agatttcaat gggcggttac ctggtccagt aactggacgt ccgcgacgac
    22921 cgttatcgat caatgcatct actgcttctt gcagcatacg tttttcattt tgaacgatga
    22981 ttccaggtgc attcaagtct aacaaacgtt tcaaacggtt attacggttg attacacgac
    23041 ggtaaagatc attcaagtca cttgttgcaa aacgtccacc ttctaattga accattgggc
    23101 gtaaatctgg tggaataaca gggataacgt ccataaccat ccagcttggt tgatttcctg
    23161 aagcgcggaa tgcttctaaa atatccaaac ggcggattgc acgtgtacgt ttttgtcctt
    23221 gtgcagattt caattcttct ttcaattcgg ctgcttctgc atctagatca acattatcaa
    23281 gtaattgttt aatcgcttct gcacccatag cagcttggaa ttcttgtcca tattgttcac
    23341 gtttttcgcg atattcgcgt tctgtcaaca attgtttttt ctctaaattc gtatttcctg
    23401 gttcgatgac tacatatgaa gcaaaataaa tgatttcttc caaagcgcgt gggctcatgt
    23461 ctaatacaag acccatacgt gaaggaatcc ctttaaagta ccagatatgt gaaacaggag
    23521 ctgctaattc aatatgtccc atgcgttcac ggcgaacttt cgaacgagta acttccactc
    23581 cgcatcgatc acaaacgatt cctttataac ggatacgttt gtattttccg caggcacatt
    23641 cccagtcttt tgtaggacca aaaatccgct cacagaacaa cccttcgcgt tcaggtttta
    23701 aggtacgata gttaatcgtc tcaggttttt ttacttcacc gtaagaccag cttctgattt
    23761 tttcgggaga agccaaacct atttgcatac tttcgaattt atttacatcg atcaaaaggg
    23821 gttccctcca tttcatttaa tggcaaaccg caagacggct gatcttcgac cgccttgcag
    23881 ctcatagacc ggattagtct aaattatctt ctgcggtttc aaagttttgg atgacatcgt
    23941 ctttttcatc ttccacttgt tcagcagctt tcttttctaa ttctttggca gtttgctgtt
    24001 cggcaaattt tgtcaaagca tcaacagtga tcaaatcgtc gtcttcatcg tccatgtcgc
    24061 gaagttcgat ctctgtttct tcgatatcca agacacgcat gtccagacct agtgattgca
    24121 attctttcac caatacgcgg aatgattccg gtacacctgg ttttggaatt ggttcaccct
    24181 tgacgattgc ttcgtatgtt ttcacacgtc cgactacgtc atctgatttg tatgtcaaga
    24241 tttcttgtaa agtataagcc gcaccgtaag cttccagtgc ccatacttcc atttcaccaa
    24301 aacgttgtcc accaaattga gctttacctc ccaatggttg ttgtgtaacc aatgagtaag
    24361 gtccgattga acgtgcatgc aatttgtcat cgaccatgtg agcaagtttg atcatgtaca
    24421 tcacaccgac agacacacgt ccatcaaatg gttcacctgt acgtccgtcg tacagaacag
    24481 ttttcgcatc gctggccata ccggcttcac gaactgtttc ccatacgtct tcatcacttg
    24541 caccatcaaa tactggcgtt gctacgtgga tacctaactg acgtgcagcc atacccaagt
    24601 gtaattccaa tacttgcccg atgttcatac gtgaaggcac ccctaaagga ttcagcatga
    24661 tatcgattgg tgttccatca ggtaggaatg gcatatcttc ttccggcata atacgggata
    24721 caaccccttt atttccgtga cgaccggcca ttttatcccc ttcatggatt ttacgttttt
    24781 gaacgatata aacacgaact aacatgttta cgcctggtga caattcatct ccagcttcac
    24841 gagtaaagat tttcacatcg tgaacgatac cgccgccgcc atgtggtaca cgtaatgatg
    24901 tatcgcggac ttcacgagct ttttcgccaa agatcgcatg caatagacgt tcttctgcag
    24961 ataattctgt tacccctttt ggcgtgactt tccctacaag caaatcgcca tcttggactt
    25021 ctgcaccaat acggatgata cccatttcgt ctaaatcttt taatgcgtct tccccgacat
    25081 tagggatttc gcgtgtgatt tcttcaggtc ctaattttgt atcgcgggct tctgattcat
    25141 attcttcaat atggatagaa gtgtatacgt catcttttac caaacgacgg ctcatgatga
    25201 tcgcatcttc atagttgtac ccttcccaag tcatgaaacc aactaaaacg ttttggccta
    25261 gagccatttc gccttgttcc atagatggtc cgtctgccag agtatcccct ttttcgactt
    25321 tctcacccaa atgaacgatt ggacgttggt tgtagcttgt tccggagttt gaacgacgga
    25381 attttgttac atcgtattta tctaatgcac cgttgtcgcg gcgtacgcgg atttcagaag
    25441 catcaacgta ttcaacgaca ccgtcatgtt tacataacaa agcagctcct gagtcatgcg
    25501 ctgatttgta ttccatacca gtaccaaccc atggtgcttg aggattgatc aacggcactg
    25561 cttgacgctg catgttggca cccatcaagg cacggttgga gtcatcgttt tccaagaaag
    25621 gaatacatgc tgtcgcaacg gcaactactt gtttaggtga tacgtccatg tagtctactt
    25681 tatcaataga tacttctaag ttttcacttt gagcacgagc cataacaacg tcttgagcaa
    25741 atgttccatc ttcattcaat gggctgtttg cttgggctac gatgtaatgg tcttcgatat
    25801 cagctgtcaa gtagtcgatt tggtcagtaa cacgacctgt ttcacggtct acacgacgat
    25861 aaggtgtttc gataaatccg aatttattta ctttcgcata actggacaaa ctattgatca
    25921 acccgatgtt tgggccttca ggagtttcga tcggacacat acggccatag tgggagtagt
    25981 gaacgtcacg aacttcataa ccggcgcggt cacgagtcaa tccgccaggt cctaaggctg
    26041 aaagacgacg tttgtgtgtc aattcgccaa gagggtttgt ttgatccatg aactgtgaca
    26101 actgagaaga accaaagaat tctttgatac ttgccacaac tggacggata ttgatcaatt
    26161 gttgaggtgt caatgtttca gtatcttgga tcgacattct ttcacgaacc acacgttcca
    26221 tacgtgctag cccgatacgg aattggtttt gtaaaagttc accgactgaa cggatacgac
    26281 gatttcctaa gtggtcgata tcatcgacat tgccgatacc ttccatcaag ttcaagaaat
    26341 agctcattga agcaatgatg tcggctggac gaaccgtttt tacagctgct tctggatagc
    26401 cgtttccgat cacgtttact tcacgttcag ggtctcttgg tgaaaatact ttgatgactt
    26461 ggacagtcat tggatctgtg accacaccgt cttctgatgg atagtaagtc actgaattta
    26521 ggccattatc aatgaaagga gctagtgttt ccatgacttg gtgagtcaga actgttcctt
    26581 tttcaacgat gatctcgcct gtttctggat caactaatgt ttcagctaat gtcagattca
    26641 atagacgtgt tttcaaatct aattttttgt ttactttgta acgaccaacg tttgctaagt
    26701 cgtaacgttt tggatcgaag aaacgagcat tcagcaagtt acgtgagctg tctgctgttt
    26761 ttggttcgcc tggacgcaaa cgttcgtaaa cgtctttcaa accttcttct gttcttgagt
    26821 cgcttgcgtt tttatgcaag tctttttcga ctgtattgcg taatgtttcg ctgtcgccga
    26881 agatttcaaa gatcgtatca tcggaaccaa atcctaacgc acgaactaat acggttaaag
    26941 gaatcttacg tgtacggtcg atacgtacat aagagatgtc ttttgcatca gtttccattt
    27001 ctagccaagc tccacggtta gggataacag tagaaccgaa tccttctttt ccgtttttgt
    27061 cgacttttcc atggaaatag acacctggtg aacggactaa ctgagaaacg attactcgct
    27121 ctgccccgtt gatgatgaat gttccttgtt ctgtcattaa tgggaaatca ccgaagaata
    27181 cttcttgtgc tttgatttcg ccagtctcgc ggtttgtcaa acgcaatgtt acatgtaatg
    27241 gtgcagagta gtttgcatca tgcgcacgag cttctgctac tgtatacttt ggttctttca
    27301 attcataatc tacaaattcc aaagatagat tgccattgaa gtcatcgatt ggcaaaatgt
    27361 cttcaaacat ttctctaagt ccctcatcta agaaccattg gtaagagtct gtttgaattt
    27421 cgatcaaatt tggtaattcc aacacttcac tgattcttgc gaaacttcta cgttcgcgat
    27481 gttttccgta ttttactacg tgtccagcca agctcttcac ccctctaaaa tgtcattcaa
    27541 aaagactacc cgatcaattg ttacaataat atttcaaata ttatttttag gtaacaacag
    27601 acattttttg cggcttttag gcaaaaaaaa accaaaaata gaacattctc ttgaaacctt
    27661 acttcgtgag cttctacttt tgtttcctta tattaaagcc gaaacggaca atatttcgtt
    27721 tctgcctttt cttcatcaga tagtttttgc aacttcatat actacactta ttttctataa
    27781 aagtcaacac ttcctggcgt ttcttctgct ttttcgcaac attttcttaa tgttttcaat
    27841 tttatgtttt caattatctg acaattctta attgacagac ctttttatct cggttatact
    27901 ttagaaaatt cattttgtgg aggaacttct atgcgaacca acatcaacca aatcgcacca
    27961 acacaactga ataactttta ctttagctac tttagatagg tttgtattca tcatgatgga
    28021 tacaaacgtt aaacgacagt ttgtatctat gatggctaaa gaactttgta ttgcagcaaa
    28081 aagccacgta gacaaaatgc taagtggctg agcagtgaaa atacaaagta aaagtatcaa
    28141 gcattctact ttttcaattt attttccgac ttttcaatcc atttggacat tttgtctgaa
    28201 tggatttttt gtttcccaaa attaaggagg aaaaagaaat gaaaaaaaat ataatcatcg
    28261 gattaggcgc tgccctgcta ctagctttag gaggttgcgc acaaaaagac aattccgcag
    28321 aggcatcaga tggacaaaaa acaattggcg tattgcaact tgtacaacat aactcgttag
    28381 atgctgccta taaaggattc aaagaaggat tagcagaaga agggtataaa gaaggcgata
    28441 atctaacaat cgattatcaa aatgcccaaa acaatcaaga caacctgaaa agtatgagtg
    28501 agaaactcgt caaagaagat cctgatttgc tacttggaat cgccactcca gcagcacaat
    28561 ctttattaaa tgaaacaacc actatcccga tgactgtcac agcggtcaca gacttggaac
    28621 aagcccattt agtcacatcc aatgaaaaac ctggcggcaa tgtgactggc acgacagata
    28681 tggtgccaat cgaaaaacaa atcggcttac tgttaaatat cgtgccagac gccaaaacaa
    28741 tcggcatcat gtataacaac ggtgaagcaa actctaagat ccaagctgat ttggcagaaa
    28801 aagaattgaa aaaagcgggc gtcaaagtca aagtactgac agcaaattcc acaaatgatg
    28861 tccaacaagt gacgaaaagt ctagctaaag atgtagatgg aatctatatt cctactgata
    28921 acacgttcgc aagtgcggca agtgttgtcg gtgaagtcgc aaaagaaaca aaaacacctg
    28981 tcgttgctgg cgcagttgat caagtcaaag acggcggtct agcaacagta ggaatcgact
    29041 acgaaagctt gggtaaacaa acagggatca tggcagcaaa aatcttagac ggcaagaatc
    29101 caagtgaact tcctgtcgaa acagcgaatg atttggaact gtatgtcaat aaagacatgg
    29161 ccaaagcatt aggcatcaat ccagacagca tcaaagaagc tgaataaaac acaggaggat
    29221 tctcatgaca aatttgctct ctcttttctt atcatctgca tcgcaagggg tcttatgggc
    29281 cctacttgcg attggtgtat acgtcacttt ccgtatcttg gacattgccg acttgactgc
    29341 tgaaggaagc tttcctttag gtgcagggat cgcggcagta agtattacaa atggctatca
    29401 tccattgaca gcttgtcttc taggcttttt agggggtgcg gcagcagggc ttgtttccgg
    29461 actgcttcac acaaaattga aaattcccgc ccttcttgca gggatcatca ccatgactgg
    29521 tttatattct gtcacatccc gtgtgatggg tgcgccgaac atctctttat tgggtcagcc
    29581 aacgatcttt cattgggtcg agtcattcgg cataacaaaa gacaacgctg tactgatcgt
    29641 cggtctgctt tttgctttga tcgttgtatg tttacttgtt ttgttcttac gtacagaaac
    29701 aggacttgct attcgtgcaa ctggcgataa tctggcaatg agcgaagcaa acgggatcaa
    29761 cacagataat atgaagatca tcggttatat gatttctaat ggatgcattg ctctttctgg
    29821 ctccttgctt gcgcaaaaca acggctttgc tgatttaaat tctggtattg gaacgatcgt
    29881 aattggactt gcttctatta tcatcgcaga agttttgtta cgtaaccagc cactatttct
    29941 ccgtctcttg actgtcgttt taggtgcggt catttatcgt tttattttag cgttagtctt
    30001 cgaactgaat gttgaaccaa gtgattcgaa acttgcttct gctttcgtgc tagtgatctg
    30061 tctttctttc ccgcagatcc agcaaaaatt ccatttacct caaaaaaaga aaggcggtag
    30121 tgtcaaatga atgacgtctt aacattggaa aatctccatc atacctttga aaaaggaacg
    30181 atcaacgaaa atcatgtcct gaaaggcatt aatctttcct tagatcccgg cgacttcgtg
    30241 acgatcatcg gtggaaatgg ggctggtaaa tctactttgc tgaacagcat cgccggtaca
    30301 ttttctgtcg atcagggtca aattttattg aatggaaaaa atatcacgaa aaaatctgtt
    30361 gtcgaacgtt cgaaatccat cagtcgcgta ttccaagatc ctaaactagg gacagccgtt
    30421 cgtttaacgg tagaagaaaa tcttgctctt gctatgaaac gaggaaaaaa acgcggcttc
    30481 ttccgtggtg tcaaaccaca agaccgaaca tttttcaaag accagctggt tcggctaaat
    30541 ttggggttgg aaaatcgttt atcctcagaa atcggtttgc tgtctggagg acaacgtcaa
    30601 gctattacgt tattgatggc cacattacag cgtccagaac tgatcttatt agatgaacac
    30661 actgcagcgc ttgaccctgc tacttccaaa acggtgatgg aattgacaga aaagctgatc
    30721 actgagcaga atctcactgc ttttatggtc actcatgaca tggaagacgc gatccgctat
    30781 gggagtcgac tgatcatgct gcatcaagga aggatcgtcg tagatatcca gcaagaggaa
    30841 aaacagcagc tcacagtgcc ccaattatta gcgttgttca aacaaaacag cggcacggca
    30901 ttaaaagacg atcaagtact tttatcttca taatcagaaa gaggatggga caaaaaatga
    30961 acgcccaccc tcttcttttt tctctttcct tctttcgttt ttgctttatg tatcaaccgt
    31021 tgtttcttcg aaaactattt tgttctctcc ttcttcctga tagaatggat gggaaaggag
    31081 cttgatgcca tgacaacgaa agacaaggta cttcaattat tgaaaaagac accagatttt
    31141 ctttccggag aaaagttggc acagcaactg gaagtttccc ggacaagtat ttggaaagcc
    31201 atcaaagaat tggaaaaggc aggctatcgt tttgagcacg ggcctacagg gtatcgttac
    31261 ctgccctctg acgtactaga tgcaaaagaa atccagcaag agctacggtc gtttattcct
    31321 gatttgaaag tacaaacgat gttgacttct gaatctacga tgaaagatgc caaactggca
    31381 gcagctcaag gagattctgt ttcgaagtta tttgttgctg atacacaaga acaagctcgt
    31441 ggacgttttg gacggccttt tttcgctgaa cctggacgag gaatctacat gagtctcttg
    31501 ctccatccag acaaacggtt tgatgaattg cctcaatata cgatcatcgc agccagtgcc
    31561 tgtgtgaaag ccattgaatc tgtaaccggt cgttctgctt cgatcaaatg ggtcaatgat
    31621 atctacatgg atggaaaaaa agtctgtggg attttatctg aagcagtcag tgatatggaa
    31681 agcggcagga tcagtcatat catcattggg atcgggctta atttttctgt tcctcaaaat
    31741 cattttccag ctgatctcca agaaaaagcc atctctgtct ttcccgacgg acaagctagt
    31801 gtcacaagga atcagctaat cagcaaaatc tggcaagaat tgttcactta tctttctcac
    31861 ccagaaaaag cgttggactt ctataaagaa aaatcattcg tattgggaaa aaccgtgtcg
    31921 ttcaatcaaa aaggaacgac ttatgtcggt cttgcaaaag aaatcggtga tcatggcgaa
    31981 ctgatcgtcc aaacaaatga acagaagatg agtttgtctt ctggcgaaat cagtctcaca
    32041 tctatccgta cgcaataatt atcagatttc acaaaaggac acctgatctc atacagtgtc
    32101 cttttttctg aattctcttc aaaagactct ccttattcta tcagctgaca gtttctctat
    32161 cctctagaaa aaggtattct aaaaatatct ataaagcatg gtacactttc tttaaatgaa
    32221 tcaatgcaaa aaacaagaga gtcatcagtt tacacgatca aaagagatag caggccgctt
    32281 cctttttcaa tcttatagat agaccatggg taacgataaa aacaaaaaag aacttgcgac
    32341 ttattttgcc gtaagttctt ttttagctag tgccttaatc tcttaatccc aacaaacgtc
    32401 tcgcatagat cacctgacca gagtgcatag cagcatcatc aataatagaa acgagacgca
    32461 ttcctcgtgt gactggtggt gtccagttct cgtcaattac ttcatctagc tcggaaggtt
    32521 ccagccttct taagtaagaa atagcaaaat cagcagctcc tcgcaagtat cctagcagat
    32581 cttctatttg attagcttgg atctgctttg cttgttccag actatgcttc caatcttgtt
    32641 cgttatctgc tatcgtcatc ggaaaccggc tttcccagtc ttgactatgc caaattggag
    32701 aagttcctgc taaatcagca atctggaaat ctaattctcg agcggtatgc caagccagcc
    32761 aagtcattga tttgatctgc ggggctctat cttccactgg aaaacgattg gcctcctcaa
    32821 tagagacgcc ttcaaacaaa cgttcaaacc gatcgtatgc ccgatccaca ctctcgcata
    32881 gcatatctaa ataatccatt tattttcctc ctttttattt atcctactta ctttttccta
    32941 attctaggat acctacttat atgttactac acacccttat tttttacact ttaacagtca
    33001 ccatcatcaa tagtttggag attgaaagaa aaagatagtg tcgaactatc aattgaacaa
    33061 gaagcaacaa tatgaacgtt tcattaatat tctttaaata aacaatgatt tttcaaacga
    33121 acagtttaag actaggagaa aaagagtata ataggaaaat gtttgaaagg aaaggaaggg
    33181 gaaaaatgaa aaaaagggca agtttactag tcctagttgc tttgttgttc aatatctttt
    33241 cgtatggtgt ggccgtttca gcggaaacat acattcctaa agaccctggg aatatttata
    33301 atgatctaac tggtaactat ggcttgttag gaattgctag tcaattccac gtatttgcta
    33361 aaaataagac aactgtgaat gcacatacag acggaaatat agcaacacat gaacttgatg
    33421 caaaaaataa ttttggtaca gagcttcatg aaggtttatt gaaccaagaa ataaattata
    33481 tacaaaaaac tgattctttc gctaattctt caggcatacc tacaggtagc acaagaatga
    33541 ataaatttgt cgttggcagt aacgtggatg tagcacttca agatggtcat cctacagtca
    33601 atggatccag gcttgatcac ttaactccag atgattttta taaagatcgt gctgggcatg
    33661 aatatattga tttcgagcag gaatttgcta aattgaatgt agcagcaaat gatcttgcaa
    33721 cgatcactcc agctaaaaca tatactgctg cagattttcc agacatgaac aatcggacaa
    33781 tcgatttaac tgatttatca gataccgggt ttttattggt aaatatcgat gcagaagtgt
    33841 taacgatgaa tacgccactt caaatcatta atccaaatga tcaagtagtt gtatttaatg
    33901 tcatcaacag ttcttctgca ttgaacgtac agtcaccaat caaatacaat gatcgttcaa
    33961 atcatgaaac agaagatttt tcggatgcga atatctcttg gaattttgga aatgagatga
    34021 ctgatttgac aatcagtgct ccttttcaag gaacaatcct tgcgcctaat gctactatcc
    34081 gcattactca aaaccaagac ggaacgatta taggaacaaa tgtgatttta gatgcagcta
    34141 ccaatcgttg ggatcctaac gaaatattta tcagcgacaa tggtacagat acgacagaca
    34201 caagcgacag tacagataca tctaccacaa gtgatagctc agattcatca acttcaagcg
    34261 acagtacaga tactacctct accacaagtg atagcacgga tac
//
LOCUS       DAFFSV010000017        31120 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFFSV010000017 DAFFSV010000000
VERSION     DAFFSV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.017"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..195)
                     /locus_tag="LR110_000727"
     CDS             complement(<1..195)
                     /locus_tag="LR110_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821729.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKK"
     gene            complement(183..1535)
                     /locus_tag="LR110_000728"
                     /pseudo
     CDS             complement(183..1535)
                     /locus_tag="LR110_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(1532..2464)
                     /locus_tag="LR110_000729"
     CDS             complement(1532..2464)
                     /locus_tag="LR110_000729"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBL4821730.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2461..3237)
                     /locus_tag="LR110_000730"
     CDS             complement(2461..3237)
                     /locus_tag="LR110_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBL4821731.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3291..6476)
                     /gene="carB"
                     /locus_tag="LR110_000731"
     CDS             complement(3291..6476)
                     /gene="carB"
                     /locus_tag="LR110_000731"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBL4821732.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6481..7557)
                     /gene="carA"
                     /locus_tag="LR110_000732"
     CDS             complement(6481..7557)
                     /gene="carA"
                     /locus_tag="LR110_000732"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBL4821733.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7562..8842)
                     /locus_tag="LR110_000733"
     CDS             complement(7562..8842)
                     /locus_tag="LR110_000733"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBL4821734.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8861..9787)
                     /locus_tag="LR110_000734"
     CDS             complement(8861..9787)
                     /locus_tag="LR110_000734"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBL4821735.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9800..11083)
                     /locus_tag="LR110_000735"
     CDS             complement(9800..11083)
                     /locus_tag="LR110_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBL4821736.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11097..11636)
                     /gene="pyrR"
                     /locus_tag="LR110_000736"
     CDS             complement(11097..11636)
                     /gene="pyrR"
                     /locus_tag="LR110_000736"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBL4821737.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11871..12782)
                     /locus_tag="LR110_000737"
     CDS             complement(11871..12782)
                     /locus_tag="LR110_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL4821738.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12779..13243)
                     /locus_tag="LR110_000738"
     CDS             complement(12779..13243)
                     /locus_tag="LR110_000738"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBL4821739.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13248..13946)
                     /locus_tag="LR110_000739"
     CDS             complement(13248..13946)
                     /locus_tag="LR110_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL4821740.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13933..14319)
                     /locus_tag="LR110_000740"
     CDS             complement(13933..14319)
                     /locus_tag="LR110_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821741.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14466..16136)
                     /locus_tag="LR110_000741"
     CDS             complement(14466..16136)
                     /locus_tag="LR110_000741"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBL4821742.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16319..16552
                     /locus_tag="LR110_000742"
     CDS             16319..16552
                     /locus_tag="LR110_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBL4821743.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16651..17082)
                     /locus_tag="LR110_000743"
     CDS             complement(16651..17082)
                     /locus_tag="LR110_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBL4821744.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17161..17574
                     /locus_tag="LR110_000744"
     CDS             17161..17574
                     /locus_tag="LR110_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBL4821745.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17612..18760)
                     /locus_tag="LR110_000745"
     CDS             complement(17612..18760)
                     /locus_tag="LR110_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL4821746.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSGTLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18882..19478)
                     /locus_tag="LR110_000746"
     CDS             complement(18882..19478)
                     /locus_tag="LR110_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL4821747.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19678..22320)
                     /gene="alaS"
                     /locus_tag="LR110_000747"
     CDS             complement(19678..22320)
                     /gene="alaS"
                     /locus_tag="LR110_000747"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBL4821748.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22708..23139
                     /locus_tag="LR110_000748"
     CDS             22708..23139
                     /locus_tag="LR110_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4821749.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23184..24533)
                     /locus_tag="LR110_000749"
     CDS             complement(23184..24533)
                     /locus_tag="LR110_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL4821750.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24603..25766)
                     /locus_tag="LR110_000750"
     CDS             complement(24603..25766)
                     /locus_tag="LR110_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL4821751.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25895..26218
                     /locus_tag="LR110_000751"
     CDS             25895..26218
                     /locus_tag="LR110_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821752.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26401..27750)
                     /locus_tag="LR110_000752"
     CDS             complement(26401..27750)
                     /locus_tag="LR110_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4821753.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27819..28772)
                     /locus_tag="LR110_000753"
     CDS             complement(27819..28772)
                     /locus_tag="LR110_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBL4821754.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28874..30064)
                     /locus_tag="LR110_000754"
     CDS             complement(28874..30064)
                     /locus_tag="LR110_000754"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBL4821755.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(30709..>31120)
                     /locus_tag="LR110_000755"
     CDS             complement(30709..>31120)
                     /locus_tag="LR110_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HBL4821756.1"
                     /translation="FSIIENMSFSKNLSEAAELSLEGKIVQDADRIEALGAIGILRTA
                     YFGGGHGHPIFDPELYPQTFKDKENYRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKR
                     RETFMREFLEEFFLEWSIDDENFTPDNWIKNNKK"
BASE COUNT         8749 a         6822 c         5463 g        10086 t
ORIGIN      
        1 cttctttctg ctaaccagtc ttagaaaaat gaattttaaa aaaatcaaaa actgatcacg
       61 atagaagttt cttttcatac ctgatgaaaa gatgcttctc gcaatcagtt tttggatttt
      121 tgctattcca attgatgatt ctggagacac aaatcggatt tctctatctt catcaagcgc
      181 gcttaaatct gccaattttc aggatctttt ttccattctt ttagcagagt taattcttgc
      241 tcatcgatat aacgatcttc taaagctgct tcaatcaata cagaataatt ggtcaacgtc
      301 ataagaggga tttcggcttt ttcgaaattt gcttttcctt ttggcaactc atacgtaaag
      361 atagccgcaa caccaaggat atccgcacct tctctttttg ctgcttcagc tgcttccaag
      421 acacttccgc ctgtagaaat caaatcctcg atcacaacca tcttctgacc ttcaaatata
      481 cgtccttcga tttggttacc tttcccatga tctttcgcct tacttcgaat atagaccatt
      541 ggcaaatcca gtatttccgc tacccatgcg gcatgaggga ttcctgctgt tgcagttcca
      601 gcaatcactt gtacttcagg gtaagcttct ttgattttgc ttgctaatcc ttttgcgatc
      661 tcttttcgga cttttggata gctcatcgtg attcggttat cacagtaaat tgggcttttg
      721 attccacttg cccatgtaaa aggattactt ggatttaaaa atactgcttc aatttctaat
      781 aagtcttttg cgatactttg ttcaacattc attttaggtt agctccattc tgtttgaatc
      841 gtttgatatg ctttataagg attagttgac tgagtgattg gtcgacctat aacgataaaa
      901 gttgagccga tcttttttgc ctgagaaggt gtgactactc ttgtctgatc tcctgcgact
      961 gttccttctg gtcttatacc cggagttaag caaacaaatt cgtctcctgc catttctttg
     1021 atcgcttcta cttcccaagc agaagaaacc acaccatcta atcctgcttt tttggcacag
     1081 ctagcataat ggatcacact tttttctaaa gaaacttcga ttagttgatc ggcatgcatt
     1141 tcttcttcac ttgtagaagt aagctgagtg atagctagaa gtagcggacg ttttccacct
     1201 tctggagtcc cctcttctaa tcctcgcatt gccgcctcca tcatacgaat accgccagcg
     1261 gcatgaacac aggtgagatc aacaccaagc tttgccaatc cccgcattgc tttttcgacc
     1321 gtattcggaa tatcgtgaag tttcagatct aaaaatacat tatgaccagc ctcttttaga
     1381 taacgtacga tttcaggtcc ttcttggtag aacaattcca ttccgatttt cacaaacaat
     1441 ttttcttctt tcgggaaaaa tcgaagaaag tcttctacct cttgcctagt tgaaaaatct
     1501 aatgcaataa ttggtcgttg gtctagttga ttcatcgttc acgttcctct ctcacttctt
     1561 tgattaatct ttccagcgat tcgatgccta gttcgctcat tcgcacaggg agctctttaa
     1621 ttagtttagg acagatatac ggatcggtga aattcgcggt ccctatagct acagcacttg
     1681 ctccagccat aaacatctca agaacatcat ccactgtctg cacgccgccc atcccaatga
     1741 ttggaaggct ggatatactt gctacttggt gaatcaaacg aatagcgaca ggcttgattg
     1801 ctggtccgga gagtccacct gtttgattgg ctaaaatcgg ttttcttgtc ttaaggtcaa
     1861 tccgcatacc aagaagagta ttgatcatcg taaacccatc tgcccctcca gcttcgattg
     1921 cctgagcgat tggaacgatg tctgttacat ttggggacaa tttgacatag acaggaacag
     1981 aagaaacttt ctttactgct tgtgttaatt gaaaagcaac atcagaatct gttccaaaag
     2041 caattccgcc atgtttgaca tttgggcaag aaatgttcag ctcgattgct ttcacatttg
     2101 gtgcttcgcc aattttgcta caaacttcta catagtcttc ttcacaagca ccagcaacat
     2161 ttgcgatgat gggtaactca ttgtactcct ctaattctgg aagaagttta tgcataacaa
     2221 cgtctagacc aggattttgc aaaccaattg cattcaacat gccagatggt gtttccgcca
     2281 ctcttggcgt gggattgcca aagcgggcat ttggagtcgt tgccttgacc atgatcgatc
     2341 caagctgatt caaatcatag tattttccgt attctttgcc aaaaccgaaa cagccgcttg
     2401 ccggcatgat aggatttttt aattctaggc caggaagttt gaccgctaaa ggattctcat
     2461 tcatataatt acctctccta ttttaaaaat cggtccttcg tcacaaactt tcacactttt
     2521 tgttcctgtt tcatcttccg gcgtatgaca aacgcaagca tagcaagccc ctatcccaca
     2581 tgccatgcgg gattctaatg ataactggac attttgatgc gaagaaaaca gctgttccac
     2641 tgtcttcaat aacccgtttg aaccacatgc aaaaacagct gtaggttccg tcttcgttcc
     2701 taacaataaa ttaccgacat ttccgtgcat accgtatgtt ccatcgtcag tagcgattct
     2761 tgtttctcct agacgattaa attctttttc ataataaacg acttcatgtg tggcaaaacc
     2821 taaaaaatgc gttggcttaa ttcctttttc gactaactgt tttgataact catatagagg
     2881 agggacaccg attcctccac cgattataaa tacttcgtct cctgtatgaa gattggacaa
     2941 atcaaaacca ttgcctaaag gacccatgac atctaaatgg tctccggcag aaagagtaga
     3001 gaagactcta gtcccctcac cttccacccg ataaatgata cggcaagtat ttgtttcgtg
     3061 gtcgatatga ttcaagctaa tcggtcggcg taatagcaag tctgcacgcg gcacgagaat
     3121 atgcaagaac tgtcctggtt gctgcatttc ttttactaga tcacctttca atgtcatttc
     3181 aaaaatcttt ggtgccagct gtttttgctg aacgatttcc atcatttctt gtttcatctg
     3241 tcccctcctg caacggtttt tcgtcttttt gtcttaacat gtgctcgccg ttaaataggg
     3301 ttagttgaaa aagcacggga ttccatcact cgaacgatcg catctgctgt gtctaaagaa
     3361 gtaaacaatg gaataccatg ttcaactgct tctcttcgga tgatgaaacc gtctttagaa
     3421 gcatttcccc gatctttatc aatcgtattg acaaccactt gtgttttacc atctcttatt
     3481 aagtcgacta catttttttc tgctgcttcc tcagaaattt tcgctactgt ttcaacgacc
     3541 agaccatttt tttcgaaata agcagctgta tgtttcgttc ctatcagact atagccaatt
     3601 tctgtaaaac gtttagctaa ttctagtgct tcttcttttg tttcgtccgc aatcgtaaat
     3661 aatacggctc catattcagg caagtgtaat ccagaagctt cgaatgcctt gtacaaagct
     3721 ttgtctaatt gtctgtcaga tcccattact tctccagtcg atttcatttc aggccctaag
     3781 taagtatcaa ctttctgcaa tttcgtaaaa gaaaagacag gtgctttaac atgaacttct
     3841 tttgtttctg gataaagtcc gttttcataa ccaagtgtct ttagtttctc tcccaaaata
     3901 gctttcgtcg caatttgtgc cattggaatt cctgtcacct tacttaagaa tggaacagtc
     3961 cgacttgctc gaggattgac ttcgatcaca taaacctcat tttcatggat gacaaactgg
     4021 atattcatca tcccgacaca attcaaacct aatgctaagc ggatcgtgta atcttcgatc
     4081 gttttcttga tcgattcaga taatgtttga ggtggataca cagccattga atcccctgaa
     4141 tggacaccag cacgctcaat atgctccatg atccctggga tgagtactgt ttcaccgtca
     4201 caaatcgcat ccacttcgca ttcaccacca agcaagtaac ggtccactag tactggatgt
     4261 tctggagatg ctttcacagc atgttccatg taatcttcta aatctcgctg gttttcaacg
     4321 atttccatcg cacgtccgcc taatacatag cttggacgaa caagaactgg ataaccaatt
     4381 ctcgaagcga taaccacggc ttcttcttta cttgtagcag tatcccctaa aggttgagga
     4441 atatccaact ctttcagcgc ttgctcgaat aaatcacgat tttccgcacg atccaagtct
     4501 tcgatcgttg tccctaagat ttttacacca gcagcagtca aaggttcggc taagttgatt
     4561 gctgtctgac caccaaattg aacgattact cctatcggct gttctagctc gatcacattc
     4621 atcacatctt cgaaggtcaa tggctcaaaa tataatttat ccgaaataga aaagtcagtc
     4681 gaaactgttt caggattgct gttcatgatg attgcttcgt atcctgcctg ctggattgct
     4741 ttgacagaat gcacagtcgc atagtcaaat tcaacccctt gtccgatacg gataggacca
     4801 gatcccagta ctagaattga gggcttttct gatcgtgaac tttcattttc atattcatag
     4861 gaactataaa aataaggtgt ttttgattcg aattccgctg cacaagtgtc aaccatctta
     4921 tatacaggaa tcaaatcatt ttcttttctc atctggcgaa cggcttcttc gtttatttcc
     4981 cacagctccg ccactttttt gtctgtaaaa ccgttttgtt tcgctgtttt caatatcttg
     5041 tcatccaacg gctgctgttt caactgctgt tcgatttcta cgatatgcaa cagtttatct
     5101 aaaaagaata aatcgatttt tgtcaaatcc gctaattctt ctatggtgta ccctcttcta
     5161 atagcttcag ctacataaaa caggcgatca tcttgtgctc gaaccacttt ttggattagg
     5221 tcatcgtccg tgatgtctgc caactcagga agttcgatat ggtgtgtacc gatttctaaa
     5281 gaacgcacag ctttcaataa agactcttcg atattccgac cgatagccat cacttcacca
     5341 gttgctttca tctgcgtgcc taaaagccgt tctccgtttt caaacttatc aaacggccag
     5401 cgaggaattt tagctacaac ataatctaat gcaggttcaa attctgcaaa agtcgttcct
     5461 gtaacaggat ttttcatttc gtctaatgtt aatccaagcg ctattttagc agctagctta
     5521 gcgatcggat agccagtcgc cttacttgct aacgcagatg aacgagaaac acgcggattg
     5581 acttcaatca cataataatt aaaactatgt ggatcaagag cgagttggac attacagcct
     5641 ccctcgatct taagtgctcg aataatcgaa agagaagcat cacgaagcat ttgatattca
     5701 tggtcagaca atgtttgact aggggcaaat acgatagaat cacctgtatg gatacccacc
     5761 gggtcaaaat tttccatatt gcagacaacg attgcattat cagccgaatc tctcatcact
     5821 tcatattcaa tttctttaaa acctgcaata cttcgttcaa tcaaacattg ggtagcgggg
     5881 gaaagactta atccattttc tgatacgata cgtaattctt cttcattttc acacatacct
     5941 ccaccagttc cgcctaaagt aaaggctgga cgaacgatga caggatagcc gattttattt
     6001 gcaaaatcta cagcttcttc tactgtattg acgatttcag actcggggat tggctgattc
     6061 aattctttca tcaactgttt gaatagatca cgatcttctg cttgatcgat cgcagaaagc
     6121 tttgttccta gaagttctat gttcaattct tctaaaatac ctgatgctgc cagctccatt
     6181 gccatattga ggcctgtctg tccgcctaaa gttggaagga gagcatctgg acgctctttt
     6241 cgaagaatcc ttgatacaaa ttcaagtgtg atcggttcga tatatacttg atctgccact
     6301 tctttatccg tcatgatcgt cgcaggatta gaatttacta aaaccacttc atatccttct
     6361 tcttttaagg caaggcaggc ttgtgttcct gcgtagtcaa attctgcggc ttgaccaatg
     6421 atgatcggac cagatccaat caccataatt ttcttgatat ctgtacgttt cggcatctgt
     6481 ttactgctcc tttcctgcgt ccatcatttc cataaattca tcaaataaat gtagtgcatc
     6541 atgcggacca ggggcagcat ctggatgaaa ctgcacactg aaagcgggat aatctctgtg
     6601 tttgactcct tctactgttc cgtcatttac ttctacatga gttacgatga gtttttctgg
     6661 atcaatcgtg gcttcatcga ctgcatagcc atgattttga gaagtaaaat caattcttcc
     6721 ggtcgctact tctcgtaccg ggtgatttaa tcctcgatgt ccgaatttca ttttataggt
     6781 atctgcaccg ttggctaaag caaataactg gtgaccgaga caaataccaa aaatcggtac
     6841 ttttccttgg atttttttga tcatttcgat tgcttctggc acatcttttg gatcaccagg
     6901 tccattcgtc agcatgactc cgtctggtga tagcgctaaa atcgtctcgg catctgtgtt
     6961 atagggaagt actgtgaggt tacagtttct tttactcaat tctcgtaaaa tactatgttt
     7021 caagccgaag tcaatgacaa tgactttccg acctgttcct ggacttggat aaggtttgtt
     7081 agttgagact tgctgcactt gattggttgc tagaacggtt gctttcagct gatcaaatgc
     7141 atgttcgaag gaatctccag catcaactat actagctttc atcgttccga cttcacggat
     7201 tttgcgagta agggcgcgcg tatcgatacc tgatattcca ggaattcctt tttgtttcaa
     7261 aaatgaatct agcgtcattt ggtttcgcca gtttgaagct acacgtgcat gttcttttac
     7321 tactactcct ttgcatgttg gagaaatcga ttcataatca tctctattta ccccataatt
     7381 acccaccata ggataggtaa atgtaatgat ttgaccgtta aaactttgat cagtgatagt
     7441 ttcttgataa ccagtcatac ttgttgtaaa aacgatttct ccgaagacac tagcatctgc
     7501 accaaaagcc tttccttcga aaactgttcc atcttctaat ataagcaaac gttccatact
     7561 tttattctcc cttctgccac acaagttttc cgttgacaaa agtcatttgt gtttcgcctt
     7621 ttactttcca gccgataaaa ggtgtattct ccccttttga taaaaagtct tctttatcga
     7681 tcgtgcaagt ttgtgtaata tcaaaaactg ctacatcagc gggggcacca accgttagcg
     7741 ttcctgcatt caaaccaaaa atctcagccg gtttaactgc caaccaattg atcacttgtt
     7801 ccaacgtgaa tcgaccagtt tctacaaaat gagtatagat cagttgaaac gctgtttcgc
     7861 ttcctacgat accaaatggt gatttcatga aactttggct tttttcttct aatccatggg
     7921 gggcatggtc tgtagcgatg caatcgatcg taccgtcaag caagccttcg atcaaagctt
     7981 ctctgtcctc ccgtcctctt agcggcgggt tcattttcca aaagccgaaa tcttcgggaa
     8041 tatcttcgtc gatcaaaata agatgatggg gtgaaacttc tgctgttacg tggattcctg
     8101 ctttttttgc gtctctgatt acacggacac tttctttagt agaaacatgg caaacatggt
     8161 aatgaacacc agttgcttca gctagcaaca gatctctagc aatttgagaa gactcggtta
     8221 cgcttaagat cccgggaaga ccaagttctt ctgccttctt gccagcgtgc ataacgccgc
     8281 caaataataa agattcatct tctgtatggg ctactagggc tttattgttt tttgcagctt
     8341 ctttcatagc cagatacatc gtcccagctg tttggacacc tacaccgtca tttgtgaaag
     8401 caaaagcacc ttcttcaatc agtgcttctt gatcaactaa tttctcactt cgcagatttt
     8461 ctgtgattgg cgcatactgc aataccttca caactgcatc ttttctaatg atgtcatata
     8521 cttgtctcag cttttctgcc gtatcaggta caggatttaa attcggcatc gcgcatacag
     8581 tagtgaaacc gccacgagca gctgagcgag tcccagcttc gatcgtctcc ttgtatgtaa
     8641 atcctggctc tctcaaatga acatggacgt ccactaaacc aggtgtgatc aattgtccct
     8701 ttgcatcaaa cacttcatca aattcggctt ctgagaatcc tgtaccgatt gctttgattc
     8761 gcccatcttc tatccaaatt tctgctggtg tcgtcatatt ttttcgtgta ttgatctgac
     8821 cgtttttaat gagtgttttc attttttcat cctcctataa ttacgctttt ccatttagca
     8881 ctgcttctaa gattgccatt ctgacgaata ctccattgct catttgctga acgatccgcg
     8941 actgtaatcc ttccactaat gaatcagcta attctacatc acggttaact ggtgctgggt
     9001 gcataatgat tgcatgtttt tgcaacttct tcgcacgctc aactgttaac ccatgttcct
     9061 gatggtagcc ttcttttgag aaactttctt ttccatcatg gcgttcgtgt tgcacccgaa
     9121 gcatcatcat gacatcaact tgatctagta gttcatccaa tggcatataa tgtccgtaca
     9181 cttcgaattc atcgtcgtac cattctctag gacctgagaa gaatacttgt gcgcctagac
     9241 gttttaacat ctgcatattg gatttagcta cgcgagagtg agtgatatca cctacgatgg
     9301 caactttcag accttcaaaa tgaccaaatt cttcataaat ggtcatcaaa tcaagcaagc
     9361 attgggttgg atgctgtccg cttccgtctc ctccattgat aatcgaacac tggattgttt
     9421 tactttgtat cagttcgtca taatagttct catcaccatg acggataacc gctacgtcta
     9481 cgcctaatgc tgacatcgtc agtactgtat cgtatagtgt ttcccctttt tggacagagc
     9541 ttgtgcttgc ttcaaattca atcacttcta gccctagttt tttctccgct acatcgaagc
     9601 ttttatgtgt gcgagtacta ttttcgaaaa agaggtttgt agcaaagtac tggcgctttt
     9661 gcggagtcca attagcacct tgcttgaatt cctgccctct gcggatcaag cccataactt
     9721 cttgatcagt aagtgcctca acggtcaata aatgctttaa gctgatacgt tctgatttga
     9781 tgatcataat gtttcctcct tagttttctt cgcttcgtga agtttctggt aagataagat
     9841 ttaaaatgat gccaagtaca gttgctaatg ccattgccga caatgtaaat gtgcctactt
     9901 caaagacgag tccgccaatt cctactacta agataactga agcaatcaac agatttttct
     9961 ttttatcaaa atcgattttg ttctcgatta aaatcttcat tccacttgcg gcaattactc
    10021 caaataagat aaaactgatt cctgagatga ctggtccagg gatacttaaa atcaaagcgc
    10081 ttaattttcc tacgaatcct aaaatgactg caaatactgc agctccaccg atcacaaaca
    10141 cgctatggac acgtgtgata gccagaacac caatgttttc accgtaactt gttacaggag
    10201 gacctccgac aaaggctgcc acgatttgtg ctaatccatc acctgtcaac gtcttatgaa
    10261 gacccggttc ttcaaagaaa ttacgtttag ttagtttatt caataccata agatggccga
    10321 tatgctctgt catcgtcaca aaagcgatgg gtgccattgt tgtgattgct ccaagatata
    10381 gctttggttc atattgtaca aagggaatct caaagttagg aacagcaaac caggcagcgt
    10441 ctacgattgg cttagtatcc acgattccaa acagcaaggc tacgatgtat ccgctaataa
    10501 tacctagtaa aatcggaatc aatccgagaa aacctcttag ccacatgttg aagaaaattg
    10561 ttaatcctaa tgtgatcagc gctacagcaa tatacttgaa atcatacgtg ccctgattat
    10621 acatagcatt tgttgctgca tttgccgcaa gtcccaaccc gataaccatg atgactggac
    10681 cgacgacgat tggaggcaag attttatcca gccatgctga accgatcttt ttgatgatca
    10741 gcgcaacaat caaatagact aatccagttg tgactgctcc ttgtgcaatc gctggataac
    10801 catctgtttt catcaaaagc tgcatcgctg caatgaaagc gaagctgctt cctaagtacg
    10861 caggtatctt tcctttagta gtaaataagt acaccaatgt acctagtcct gaactgacta
    10921 gcgcaatacc tggatcgata cctactaaga tggggactaa cacggttgct ccgaacatcg
    10981 tgaacaagtg ttgtaaactt aatcctaccc aatgtcctgg ggttgggcga tcgtggatat
    11041 ctaacaccac gtcatcatta tgaaatcctt ttttctccga catgtttctc tctcctctaa
    11101 tcttcctctt tcaaaatcat gatgcgatct tgtccatcaa gctcttgcat ctctactaaa
    11161 atctcttccg cccgagatgt tggaatgttc ttcccaacat aatctgcacg gattggcaat
    11221 tctcgatgcc cacgatcgac caggactgct aatgaaattt ttcttggtct gccaaaatcc
    11281 attactgcgt ccattgctgc tcgaatcgtt cgtcctgtat acaaaacatc atcaatcaaa
    11341 ataacttctt ttccttctaa ggaaactggg atgtcagaag aatgaagttc cggttcttct
    11401 ggattttctt ttttgtcatc tcggtataaa gtgatatcca gttctcctac aggaatatcg
    11461 atatcttcta gctgcttcag gcgttcggca attcttgatg caatgtagat tcctcgtgtt
    11521 ttgataccta ccaatacgat atcttggata ctatggtttc tctcgataat ctcataagta
    11581 attcgagtca atgctcgctt catggttacc tgatctacta cttcttttgc ttgcattttc
    11641 attcccccta cttttttgca taaaaaaact cctgccctaa gaggacagga ggaatgttta
    11701 gactgagcgt atactgatac actgatagct atactatgtc aatgagcaat ctacttatgg
    11761 cgcaccatac gtatccttgc tcccaccctt cttagcctca cgggactaaa cttaaaggaa
    11821 ttctattaag ttttatgctt tcgttgttaa ttactatacc gttatctaaa ttaaaatgca
    11881 atgttttttc ttaatttttc taaagttttt tcaaaaactt ctggaagagg tgcttcaaag
    11941 atcatctttt cgtttgtagt cgggtgcgta aaacctaaca tttgcgcatg gagaaactga
    12001 ccattgcctt tcaaagtttt cttcggtccg tatactggat ctccagctaa cggataaccg
    12061 atatacttca tatgcacacg gatctggtgt gttcttccgg tttctagttt taattcaacc
    12121 agagtaaatc cttcgaaacg ttccaataca gtaaaatgtg tgacagccga cttcccatct
    12181 tctcttatcg cctgcatttt ccggttttcc ttcgaacggc cgattggcgc attgatggtt
    12241 cctttttcgt gaggaatctc tccatggact aaagcaacat atttgcgcaa agaggtctta
    12301 tcttttaatt gctgagctaa tgattcgtgt gcctggtcat ttttggcaac cattagcagt
    12361 ccggaagtat ctttgtcgat tcggtgaaca atacctggac gaataacgcc gttgatcgta
    12421 gaaagatcct tgatatggta catcagcgca ttgaccattg tgccatgcgt atgacctgct
    12481 gaaggatgaa cgaccattcc ttgaggtttg tttatcaccg ctacatcttc atcttcgtaa
    12541 acgatcgtca acgggatatt ttctgggacc acatctaatg tttctggttc ggggatcaag
    12601 acagtaatct cgtctccttg ttttactttg taatttgctt tgactggctg tccattgaca
    12661 gaaaccgctc catttttcag ccacagctga atttgcgaac gactgtaatc ggtgaataaa
    12721 ttagtcaata ctttatcgat cctaccagat tcatggtgaa tcgtcgtttt aagttctgtc
    12781 atttttttct ttgatccttt tcatctaaaa taatataaat taacatacaa atcactccac
    12841 atacaagtgt cacatctgct acgttaaaaa tcggaaagct cataaaatct gtctggaaca
    12901 tatctactac atagccaaga cgaatacggt cgataaaatt gccaatcgca ccagctagaa
    12961 tcaaactcaa tccaatcatg aaccagcgat tacctttttc acgattttta atcatcaatg
    13021 tgagaatcac aacgactgca atgagtgtca ctatagtaaa gaaccacatc ttcccttcta
    13081 gcatactcca tgcggctcca gtgtttcgaa tatgattcag tgacaaaata cctggtataa
    13141 gatttttcgt ctctccaagc gctagattat cagtgatcca ccatttgacc aactgatcca
    13201 ctcctactag caaagcgctg aatatccaat aaataattaa caaaaaatca atccttttct
    13261 atttgtttat aaatatcacg aggtcgtacg atcccgagca gattaccatc gggattccca
    13321 tctttagtga taagtacggc ttccaaagtc ccttgctcac gaaacatcgc ttcaacctcg
    13381 taaatatgtg ttttagctgg aacaaaatga taatttgtgt actttccgtt ttggatgatc
    13441 aaatccaacg ctcgtttatt tgttaaatca accggacctt tttggctttc ttttgctagc
    13501 caaaacccga tattacgtaa agtaatcaat gctacaaatt ttcctttatt gtataacgga
    13561 aattgtgaat accttttttc tgctactatc tttaacagtt caagtaacgg aagatcttgt
    13621 tcaaaacctg ttacacgttt tgcaaatcga ggcagcacgg tttcaggctg aatcagttct
    13681 tgttcgatca gctggattcg cttgactgtc caatcatttg gctctgcaat gacaaaatcg
    13741 actgcgatcc gatcatggac gatcgcattc cttaactgag caagctgtaa aagatcatct
    13801 tcgtattttc tgatcattaa ttgttttctt tgagctaggc gtctgacaag ttcggtaaat
    13861 cccatatttc gtgcatttcc catctcgtct ctcatccatt tttcgattcg attgaaactg
    13921 attaaaaaaa gatcagagtt tgccataatt ttcctccttg atcaaatatg cccggttttt
    13981 tatatagaaa aagaacgcaa tcaacacaat aactgcaaaa aaactgaaaa ataaattttg
    14041 taggaaaacg gcccccaacc cagttgctag aaataaaaac ggatagattt gaagaatcaa
    14101 gtaattcatt gtgatcacat aagcaggctt tgcttctttc actgtataaa cggggctttt
    14161 tagccaataa aaggtgacct gcatcgtaaa gggcacttcc agatcaagcg ttatcttttg
    14221 ttcatgactg agaacactgc gtttttcatt gttgattctt agctgaatcg agctacccat
    14281 accgtaaaaa ccggtttttc ttataaatgt cgttttcatc ataccctcct attatgcttt
    14341 agtatagctc aaaatacaat aaagaaaaag aatgatccta gtgtaaacaa aattaattcc
    14401 ctatcgtcta aaaaaagaag ctgtagtcaa aatacagctc cttcgatggt agattataat
    14461 caagcttaaa acaatcccaa cacttctccg tcatttgtca cgtccatatt caatgcagca
    14521 ggttttttag gcaaaccagg catcgtcata acatctccag tcaaggctac taaaaatcct
    14581 gcccctaatt ttgggatgat ttcgcggatc gtgatggtga aatcttccgg tgcacctagc
    14641 agtgaaggat catcagaaaa tgaatactgt gtttttgcca tacaaatcgg cagtttgtcc
    14701 catccattct tcgtaaactc ttctatttgt ttttgcgctt ttttgctgaa aacaacacca
    14761 tttcctccgt aaattttctt gacgattgtt tctgtctttt cttgtatcgt ttcttctagg
    14821 cggtataatg gcttgtaatt agcttctttt ctatcaatca atcgtactac agcttttgct
    14881 aaatcaacgc cgccttctgc tccatctgcc catacactcg ctctcttggc aagaatcccc
    14941 tgattttcac acaagtgctc caacagtgtg agttcattgt ctgtatctgt tacgaattca
    15001 ttgattgcga cgatcacagg tagctgatat tgttccatat tacgtatatg gcgctttaaa
    15061 tttgcaaaac ctactttcaa agcatcaaga ttctcatttt taagttcatc ttttttcatt
    15121 ccgccatgca ttttcaatgc acggacagtg gcgacaatca ccacagcatc cggcgctttt
    15181 ttcaagttag gtactttgat atctaaaaat ttctctccgc caagatctgc accaaatcct
    15241 gcctctgtga caacgtaatc tgctaatctc aatgctgtct tggtagctaa aatactattg
    15301 cagccatgag cgatattggc aaacggtccc ccgtggacaa aagctggtgt tccgtaaatt
    15361 gtttgtacaa gattcggctt gattgcatct ttcagcagta atgccagagc gccttctgct
    15421 ttcaagtctc cagcagtcac aggctgacgg tcaaatgtat agccgacaac gatccttgca
    15481 agacgagcct ttaaatcttc aaggtctgcc gcaagacaca agatagccat gatttcgctg
    15541 gcaactgtga tatcaaaccc atcttctctt ggaacacctt ggattggccc acccaagcca
    15601 acaattactt tacgcagttc acgatcattg agatcgacta cccgtttcca aatcactcgg
    15661 cgggcatcga tgttcaattc atttccttga tgaatatgat tatctaaaag agcagataat
    15721 gcattattag ctgttgtgat agcatgcatg tcaccagtaa aatgcaagtt gatatcttcc
    15781 ataggcaata cctgtgcata tcctccgcct gcagcaccac ctttgattcc catcacagga
    15841 ccaagtgagg gttctcgcaa tgcaataaca gattttttat tgattttatt gagcgcgtct
    15901 cctaacccta cagtgatcgt tgactttcct tcgccagctg gtgttggatt gatggaagta
    15961 actaagatca aatgcccctc agcgttatcc tttgatcgat tgattccaga aaaattgatt
    16021 ttcgccttat atttcccata cagttccaaa tcgtcttcct taagatctaa tttactagcc
    16081 acttcaacaa tcggttctag ctctgtttct tgtgaaattt gcagatcatt cttcatttga
    16141 ttaccccttt tcacgaactt tattcaacaa aatatcttct ttgttaagat atgccaatta
    16201 tacctttttt tattcagaaa aaaaacataa aattacaggt ttttaattta tttgacgtac
    16261 ttataaatac tctttataat gaaggctatg atcagacttt agaaaggtcg tgagtgcttt
    16321 gaccacagga attgtaaaat ggtttgacaa caaaaaagga tacggcttta tcagttatga
    16381 tgacacagaa gaaatttttg tacattttac tgcgattgaa gaagagggat tcaaaacttt
    16441 ggaagaaaat caagtggtcg aatttgagat catcgaaggg aatcgaggaa cacaagctgc
    16501 ccatgtcaag aaaggcaacg ttcagactac cgatactagt atgcatgaat agataaacaa
    16561 atagagtgag acgctaaacg ggatgaaccg tacgtctcac tcttttcttt tcctgtaaaa
    16621 attcgttttt ttaggttctg atttttaaaa ttagcttttt ctttttttcg tataaggata
    16681 aggaattgat tctgatttgg gtccgttttt ttcaaaatga tcaaaaagga ctacttgatc
    16741 tttttttcgc atcatgaaaa cagatggggc tttctcatct tgcaacatca tttgcacccc
    16801 agtattagtt ggcgtcaaag atcgaatctg tccgacagat atttctcggt ctttccaaaa
    16861 agcagcataa cgtataatca cagagtttga ttttaaaata aaagaacgcc gcaaacctag
    16921 aaatagaaaa acaagaaaaa tacctaaaac aaaattgctt ttccaataag gacctgtgtt
    16981 ttctaaagaa agaatcagac taaaaaacag aatgatcaag gtcaatgacc aatagatgat
    17041 cgtatgtgcc agttcaggct gccatcgaat attttttttc attctctaac ctctcaccga
    17101 ttcagtaatg atataatctt accataacat tagtttgaac aaaaaggaga atctcttttt
    17161 atgttaaaag tatacattga tgcctctaca aaaggcaacc ctggtcctag tggaggtgga
    17221 atcttgattg tctatcaagg taaacaagaa cagctatcta tcccgctttc tatagggaca
    17281 aaccatcaag cagagttcga agtcttttta aaaactttag aatttctaaa gaaaaacgac
    17341 ttacagaatg aaacgatttt ctgtttttca gacagcaaaa cactagtttc tacggtcgac
    17401 aaaaacgcaa cgaagaacga gctcttcctt ccctaccttc aaagaatcca agaacttcta
    17461 tccgaatttt ctttattgat cctgcaatgg ttgcccgaaa acaaaaataa aggagcagat
    17521 aatcttgcac gtcaggctct ccataaacaa ttaaaagaaa aaaagactct ttaaattaaa
    17581 aactttaagg agtcttttta tagtttctca tttaatataa aagataattg tgatcaaacg
    17641 taatttccaa ttggtaatct ccgatttttt cttctatttg actacgtaag attctttcta
    17701 gttctgtatc aacaaactgg aatttctcag ggaccactac atcaaattgc agcgtttttc
    17761 cttgatgaat tactctaaaa tcatgaatat taagccctgt acctgtttca agaagaattt
    17821 gctttaactg ccgcaagata ttatagtatt tttcgttatc aacaggcatt ggatccaagt
    17881 gacagacaag ttccacatcc aaagactttt taaagtcttt ttctatcaga tcaataatct
    17941 tatgagccac attcaaattc attgattcat ctacttcgat gtggactgat gcaaagcgtt
    18001 tattgggtcc atagttatgc accaataaat catgatagcc aagtatatca tcgcaagcat
    18061 ttaaatgctc ttccatttct tcaatctctg ctggcgttgg gcgacttcct aatagttcgt
    18121 atacaaaatt tcgaagcatc gtcaatccac tataaaggat atataatgct aaagcaaaac
    18181 ctatatatcc atcaatacgc catccagtta cccattcgat tgctgctgaa agcaatacag
    18241 ccagtgtagt aaagacatca ttataactat ctgttgctgt agctctcaat gttccagaat
    18301 caatggattg cgcaataacc tgatacattt tgccttgtgc aaatttcaaa aagatggaaa
    18361 tgattaggac aaaaaagacg atgggtgtta aagagacatt ttctggatgg atgatttttt
    18421 cagcagaaga ctctaaaaat tgaaatccta cataagtaat gatgatcgaa acaaataacc
    18481 cactgatata ttcgaagcgt tcgtgtccat aaggatgttc ctgatctgct ggctttgcag
    18541 caattttaaa tccgatcaaa gtcaatatgg atgaagcagt atctgataga ctgttcactg
    18601 catcagccat aatggagaca cttcctgaaa aaagcccgat cattagctta gagacaaaca
    18661 agatcagatt cgtaaccaat cctaagatac ccgctagtac tccttttttt gtccgctgtt
    18721 gcggtatttg tttctttttt tcttcttgtt tcttagccaa gttttttacc ccttctagtt
    18781 tgcttgttgc tcaggtaaaa agatggagag gacgattgtt ttcctctcct ctcttccttt
    18841 ttctatctat cgtgcgtcct attactatta aaagatggtc attattctgg tttcaacggt
    18901 gttgctgcaa ataacgcttt tgctagtaat ggtgttatga ttcccgcacc tattgcttct
    18961 ggtatcccat tgattgctgc aacccctgct aaggttttaa ataaaagagc agagtctaca
    19021 ccataggcat ctgccgttgc acctgtataa aataatccca tgaatcctaa cactaagact
    19081 gtattgacca atgaacccag tacccctgca aaggctgagc tgatcgttat tgatttattt
    19141 ttttgataaa tccagtggaa aaccaagcct gctactaagc caacaagtac tctgggaact
    19201 accgagataa tcggattcgt gaacactaat gtatcaaatg gcgttgttgg catggcatag
    19261 gcacgaacaa ttgtagcgat tccccaaaac aagccaatca ccatgccatc ttttgggcct
    19321 aatactactg cagcaataat tactgtgata tggataattg ttaaactaat gaatcctaat
    19381 ggaataaacc ccagccaagg aaccatcgct tgtacgatga tgattgctaa caagatcgca
    19441 cgtaagacta gtcgaaaggt cttatttcta ctattcattt tttcacccct ttttatttat
    19501 actaatcgta aaaatttctg tcactttcgt caactaaaga ccatccgaat ccgaccattt
    19561 gattagatgc gccatccaat tgaaaaaagt atgagggcaa gacttgtttg tcttcgcccc
    19621 cataatcatg tttcgttcaa tgtttgaact tttgtttttc tgtcaaacgt aaacgcccta
    19681 ttgttgcgca acccattctt tcgcagcttt aagtgcatct ggaataccag caggattttt
    19741 cccgccagct tgtgccatgt ctggacgtcc accaccgcca ccgcctactt tcggtgcgat
    19801 tgctttaatc aagtcgccag cttttagtcc agcttcattt gattctttcg tcattgccgc
    19861 tagcagactg acttttcctt cttgttcgtt tgctaaaaca agaatatctg aaatattttt
    19921 ttgtttccat tggtcagcta attgacgcag ctgattcata tctttcactt ttacttgtgc
    19981 tgcaatgatt gtttttccat tgatcgtttc tggatctttg aatacatcac cagcttgttg
    20041 attcgctaac ttgctagcta attgttcatt ttccttttgt agctctctta cttgctgttg
    20101 caattgttcc gcttttgaaa caacttcttt caattgaggt gatttaacaa ttgttgctac
    20161 ttgttttagt tgtttttctt cttcttcaag tagttcgtac gcttctttac ttgttacagc
    20221 ctcgattcgt cgcacgcctg caccaattcc tgattcagaa acgattttga agattccgat
    20281 gttttctgta tttgctacat gtgtgcctcc gcacagttcg attgaccagc cgccaatatt
    20341 tactactcga acgtcatgtc cgtatttttc accaaacaaa gccattgcac ccatgttttt
    20401 ggctgtatcg atatctgttt caattgtttc aactgggata gcttcccaga ttttttcgtt
    20461 gacgattctt tccatttcag ctaattcatc tgaagtgatt tgaccaaagt gggtaaagtc
    20521 gaatcgtaaa tgtccaggag cgactaaaga accagcttga tttgcatgct cgccaagtac
    20581 ttctttcaat gccttgtgca gtagatgggt tgctgtatgg tttttgatga tccgattgcg
    20641 catcgattga tcgatcaata gatgataggt ttgtccttca ctcattggtg cttttacttc
    20701 tacggtgtgc ataaattgac cattaggcgc tttttgaaca tctgtaacaa ctgctacgat
    20761 ttcgcctact tcattcatga tccatccttg atccgctact tgcccaccca tttctgcata
    20821 gaaaggtgtt tgatcaaaga tcatttgagc ttcaccttct gcgacttcat tcaaaagaga
    20881 ctcaccttta acgatgacca ataattgact atctgattct tgttctgtgt atccaatgaa
    20941 tttactttct actttgatat ctgtcaataa agcagattgt acccccattg atttttcagt
    21001 actgcgggca gagcgagcac gttcgcgttg tgcatccatt tctttttcaa aaccagcatg
    21061 atcaactttc agtccttcat cttctgccac ctcttccgtt aattctaccg ggaagccata
    21121 tgtgtcgtat agtttgaaga tgtcttttcc tgctagagtc gtcccgtttt cgttttttac
    21181 ttcttggatc acttgattta agatttccaa accttcattg attgtttcat tgaaacgttc
    21241 ttcttctgta cggataactt tttcaataaa ttctttttgt tgaagtactt caggataata
    21301 acttaccatg atttccccaa caacagggac taaacgatat aagaatgctt gttcaatacc
    21361 taattttttc ccatgcatga cagcacgacg aagcaaacgg cgtaagacat accctctccc
    21421 ttcattcgat ggcaatgcac cgtcgccaat agcaaatgaa agggcgcgaa tatgatctgc
    21481 gattactttg taagaaatat ctgttactgg gttttcacca tattttactt gtttgcttag
    21541 cttttctacg gcgtggataa taggcataaa caagtctgtc tcaaagttcg ttgacgcatc
    21601 ttgaacgatt gagaccatgc gttccaatcc catccccgta tcaatgtttt tatgaggaag
    21661 tggttcaaat gtgttatcag gcttatggtt gaattctgaa aatactaagt tccagatttc
    21721 taaataacgt tcattttctc ctcctggata gttttcagga tcgtcttctg ctaaatcgtt
    21781 ataagcttct ccacggtcat agaaaatttc cgtatccgga ccacatgggc ctgcaccgat
    21841 atcccagaag ttgtcttcta catcaataat gtgatctagt ggcaggccaa cttcttcatg
    21901 ccagatgcgt tttgcttctg tatcttttgg ataaactgtt acataaagtt tttctggatc
    21961 gaatgccatc cattttggat cagtcaaaaa ttcccatgcc cagtggatgg cttcattttt
    22021 gaaatagtca ccaatcgaaa aattacccag catctcaaac atcgtatggt gccgtgctgt
    22081 tttaccgaca ttttcgatat catttgttcg aatagatttt tgggcatttg tgattcttgg
    22141 attttctggc acaactgaac catcaaaata ttttttcaat gttgcaacac cagagttgat
    22201 ccacaataag gttggatcgt tcacggggat caaagacgca ctaggttcaa tcgtatgtcc
    22261 tttagattgg aagaaatcaa gaaacatttg tcgaacttgt gcactactta attgtttcat
    22321 gtcattacct gctttctata aattaaatac aaaaaaacac catcacagcc aaggacgaaa
    22381 actcgcggta ccaccttgat tgcaatgatg tgtaatcatt acctcttaaa ctcgataacg
    22441 ctgagctgcg tcggtatttc taccaaaata gaaaaaggga gcaatttgat tagtgactat
    22501 tcttttttca gccaagaaag aattttctgt gagtcttaaa aaacaaatca tatccttttc
    22561 gtacttattt agtatacgca ttctattgat cactgtcaat atctcaattt ttattttcat
    22621 ttccttttgg tcatactcca cgatttttgg tacgataaga ataaaggaat caatacatct
    22681 ctgctattca tgacaaggag gaatatgatg aaaaaaatag ttggagcgga tgaatggata
    22741 cgcgcagcta gctgcttttt gagatatcaa gtttttgtat tagaacaagg gattcctgca
    22801 gaactagaat tcgacggaaa agatatcccg caacaaacct attatctatt tcttgatcaa
    22861 aaaagaccag tagctacttt gcgttatgaa aagaaaacac ctacttgctt caagccggaa
    22921 cgattttgcg tagcaaaaga ataccgagga aaagggttag gcggtcagtt gcttgaagct
    22981 gctgaaaaac gggcggtttc agaaggttgt cagactagtt atgtagtagc tgaaatgacg
    23041 gctgtagatt tttatgaaaa acaagggtat catacttgca cagaaccttt tattgaagat
    23101 gaagtacttt gtatcggaat gaaaaaaagt ctagcttaaa aacaagagat tgtgacacgc
    23161 cttttgtcac aacctcttgt tttttattta tgtgaatttt tcttattttt acgagcagta
    23221 cggctttgct gtctgcgttc gattttgcgt tcttttttat ttttttctgc aattgcccaa
    23281 tcgatttttt tcttataacc aggcttgatc tttttctttt tcttcttcac aagaccaatc
    23341 agcgtgggat ctaatacatc ttttgatttt tctcgtttgg cacgtctatt acgatcgtat
    23401 gttttgacga tttccccgtt tttgatttct tttggttcaa atgagacgcc caatttttct
    23461 atcgcatcaa tggcttgctc atcatttggc gaatataacg taatggccgt tccttttaag
    23521 ccattacgtc cagtacgtcc gacacgatgg ataaagaagt ctagatcttc tggaatttct
    23581 gcattaataa catgagagac accttcgata tcgatccctc tagcagctaa atcagtcgct
    23641 acaacatatt gatactccag attctgtact tgtcgcatca ctcgttttct ctctcttggg
    23701 gtgatgtctc catgaatctt tgctactttc aagccttggt ttttcaagta atcggcaatt
    23761 tcatccacat gctgttttgt attggcaaat acgattgcta aataaggatg accaattgtc
    23821 agcagttgat aaatcatttg attctcatct ttaccttttg ttgaaatcag ccaattttca
    23881 atgtcttcag aaataactgc ttttggtttg atttcttcaa tgattggatt ttccatgtat
    23941 tttcgtaaga atggacgaag tttttctgga atagtggcag aaaaaactaa aaactgcagc
    24001 ttttctggta atcgacttgc aatttggtct acttcattta aaaaacccat atccaatgtc
    24061 atatccgctt catcgacaac aaatgcaaat gcggtgtgaa tcttcagcgc ttgttcattc
    24121 atcatgtcta atattctacc aggcgtgcct actacaacct gtggctgttg gtgatgcaga
    24181 cgatttaatt gacgctgttt gtctgtaccg ccgacaaaat tcgagacacg gatctccggt
    24241 gtagaaaatt tagcgatttg tgtagctgct tcatagatct gtgcagctag ttccctactt
    24301 ggagctgtaa taacaacttg tacttcctct ttttctgcat tgattttttc aaacaaaggc
    24361 aacaaaaacg tatgtgtttt tcctgtacca gtttgagatt gcccaatgat attttttcct
    24421 tttgcaatta aaggaatcaa acgttcctgg acctctgtgg gctgtttgaa gccctttgcg
    24481 gttaacgctt cattgataaa cggtttgaag cggaatatat caaaattatt cattcgtgca
    24541 cctcttctct ttaaattatc acgactttgg cattataaca cagacaaagc gtttttgctc
    24601 agctaatttc gagaatcatc atcctttgtt tggccaatcg tgctaacagt atatgtgtga
    24661 ctagcttttt cataaaacag aatccgtttt tttctcaaaa atgatagcaa gatatttaaa
    24721 ataaaaatac cttgtagtaa caagacaccg agagaaatga atactgcact catctgcaac
    24781 accgtattcg ttgtcgaatt catgatttgc tgaagcaact gccccaacca gcttaaacca
    24841 taaaaaagaa agtacagata cccgtagcgg atcatcaatg cttttaaaga aattcgttcg
    24901 cggtcttctt ccaccaaacg gatacgcaca atttttttgc caagtgtcca tccattggtt
    24961 ataaacggta caatcataaa gtatacccct acttctaata aaagccacca gatatgagaa
    25021 gtaactgccc ctgaatcgaa cgcaatttta aagaaaatcg ccgctaccaa attaataaga
    25081 gataaaaatg tccagtcgat caaataagcg accaagcgtc gagtcaatga gacagtcttt
    25141 cctttttcat aagaaagttc atccattcgt cttcttgtag gaagcataaa tgtaaataac
    25201 ggcgtaacga caaaaccaat gattccaccc aatgtatttg tcactaaatc attcacatca
    25261 aataagcggt atggtcttgg gtagataaaa taaagtccac ttagctgcgt caattcaaaa
    25321 aatagagaaa ggcagaaact agcaatcatc gttttcataa aagaaagccg gaaataatat
    25381 cttaaataaa tgccaaatgg tatgagtaat aaaacattaa aagctggttc caaaaataca
    25441 ctctgcttca ttgccggcag gtaggtacta ggttcagaca agaccaatac cgtgttgtct
    25501 ctgaaatgtt gccatgaagc aaataaatgc agctccatct ttggccctgt catttgggca
    25561 accgtctcct ttgccggaag tggtaagatc accaaaaaat acgcacacaa taaataaaaa
    25621 acgaaagaat acaaaataac tgcccttgtt actagaaatg ttccatactt tcgatattca
    25681 taaataaaaa aaacagatga gatcgctaag gctaaaaatg gaaaaacaat cagcgcaaaa
    25741 cgaatcggtt cgatataact tgccatatga ctcctccttg attctttgta tctctcttat
    25801 cttagcagtc ctttgctgtt tcaagcaagt aacgaggcta ttatttctca tttctttcaa
    25861 agtaaagtaa gataaacaca aagggggtgt cgagatgctg aatatgtact ttgtttttgg
    25921 cgtacctatt tttttactgt tcctttatgc tacgatcgct tatgtaagaa aaagaactac
    25981 aattcattat ttaggattta ttctcctgat tatttcgggt ttcatgctag tcttcaatct
    26041 tcagacttgg cagcaagcac ttcttgaaat ggataaaatg actccacatg ctttgtctaa
    26101 agtgcttggc taccctgtct atctgatttg gcttcccatt ttcatttctg gttgcttggt
    26161 tttattaaat atttatcgcg gcgtacgccg aatagttcag ttacgcaaat caaagtaaaa
    26221 gacagaaatc ggttgagccc gatttctgtc ttttactttg ctatttcatc gtagacaaaa
    26281 cgacatttac gtgatcaaaa ttaacgagta cttttgacat ttcttccacg tactcctcat
    26341 ttaaataaaa tattttgttt ggcagagaag tgcagttcta ataaaattcg aatgttttag
    26401 ctatctacgt tttaaataga gataaatcgg gataccagca agtgtcaata ctatccccat
    26461 cgaagctaaa aaggtctgag tgataagcgt gcttgatacg ataaaagtcc ctccaatgat
    26521 tgctactagt ggaataaacg gatacattgg aactttgtat ggtcgaaaca aatcaggttc
    26581 ttttttacga agaagaatga caccaacaaa gaccatcgta taaaaaatcc aaatgacaaa
    26641 gatcagcata tccgttagtg tatcaaaacc accgatcatc atcatgccaa tagcgatgac
    26701 tagctctaag atacctgcgg ctacaggaac tttagtttta tcatgaagtt tagcaaagag
    26761 cttgctaaaa ggcaggttgt tttcttttgc cataacataa gggagacgca ttccagtaag
    26821 ggtatagcca ttgatggttc cataaacaga aatcaagata ccaactgtga ccagtcttcc
    26881 accgaagccg ccaaaaatca tttttgcaac gtcacttgcc gcattgctgt ttcctgcgac
    26941 tccgtcgata gatgcggttc ttaagaacac cgcatttact aataaataaa cgatcatgat
    27001 tccgatgatt cctaaagaaa ttgcttttgg caaatctttc gccggctttt tcaattctcc
    27061 cgaaatattt cctacgtgta tccaaccgtc gtatgcgaac atcgttgcta gcaagcccgc
    27121 tcccaaggct gagaaaaatg aaagattttc tcctgcttgg atcggaaaca gttggaagtc
    27181 tactccttct tgtcggaaca aaccgaaaat gacgatcaca aacaaaggaa taagcttgca
    27241 gacaagcgta atcgactgaa aagcccctcc agctttagaa cctaaaaaat tgatcaacag
    27301 gatcgataca gcagctgtaa cagctacggg aacaatcatt gactgggaaa gaccaaatag
    27361 attgacgaac tgtgtaccga aaatgatcga caaggctgct acatttgctg gaaaataaat
    27421 cacgacttgc gcccagccca ataaaaaagc tgctgtattt ccgtaaatcc gttcgatata
    27481 tttaatcatc cctcctgttt ctggaatagc agctgccagt tcagctcctg ttagtcctgc
    27541 acagacagaa atcataccac cgagaaacca agaaaacata tgcaaactgg cggatcctgt
    27601 aacttctgct acgcttgcgg ctttaaaaaa tacccctgca ccgatcactg tgcccataac
    27661 agtcgataaa gcagtaaaaa atcccatcgt acgttttaat tcattttcta tttttacttc
    27721 ttttactggc gtggcttgat attcttccat cccttgtccc ctttcatacc atagccaatc
    27781 ccaaaagtat aaaaaacttt ttggatgtgg ttcttgtttt ataccgtctc taccaatttt
    27841 tctttcagtt tttccaacat atcctttgtc atcacatcta agtcgtattt cggcgaaaag
    27901 ccccattcct cttgcgcaca agaaacatct agtgaatttg gccaagattc tgctattgac
    27961 tgacgtacag gatccacatc ataatccatt tcaaaatctg gtatgatttt ttgaatagat
    28021 gctttgatct cttccgggtc aaaagacatc gccgatatat tgaaggcatt tcgatgaacc
    28081 aatcgatcag gatctgcatt catgagtttt ataatggcat cgatcgcatc tggcatatac
    28141 atcatatcca ttgatgtacc ttgatcaatg aaactagtgt actttccttc tttgattgct
    28201 gaataataaa tatcgactgc gtagtctgtc gtaccgcctc ctggcaaggt tgcataagaa
    28261 atcaatccag ggaaacggac accacgagta tctacgccat atttcacata ataataatca
    28321 catagtagtt ctccagctac cttcgttacc ccatacatgg ttgtaggacg ctgaagagta
    28381 tcttgtgggg tatgatcttt tggagtattt ggaccaaacg cgccgattga acttggcgta
    28441 aaaaatttca gctggaattc tctagcgact tccaatgcat tgaccagccc agtcacgttc
    28501 aaattccagg ctaattgtgg ttttgcttct gcaacagctg acaaaagagc agccaaatga
    28561 atcaatgtgt ccacttgata ttgttctgct atgctgcgca ttttttcgta atccattaca
    28621 tctaggatct caaagatatc gtttttctca atgacttcag gcatgcgtat atctgtcgca
    28681 atgacatttt cactgcctag ttcttgacgt agccgtgtga tcaactctga accaatttgt
    28741 cctaaacatc ctgtcaccaa tacttttttc atgatgatcc tccctgatct tttttactac
    28801 tatcttcaga tgattaacaa tatatgcgtg aacatgttct ctaattttaa atagacgaaa
    28861 aaactaaatt tagttaaata atttccattt cttttcctat tttttgataa actgctactg
    28921 cttcatcaag catttccctc gtatgttccg cagtcggcat atttctgatc ctgcctgtcc
    28981 caagaggcac agttggataa acgattggtt tagcataaat cccatgttcg atcagcttct
    29041 tagagaaagt ctgagtcact ttttcgtctc ccaaaatgac tggagtaata ggtgtttcag
    29101 acataccgat gttatagcct acttttttca attcttcttt aaaataatta gcattttccc
    29161 aaactttttc gacaagttcc ggatgttctt gcatcaaagt aatcgaagcc aatgcagctg
    29221 cagctgcacc tggagttagc gaagtagaaa ataggaatgg ccgacttcta gctttcaacc
    29281 agtcgattag tgttttcgat ccagctacat aacccccaac aacacctata gctttcgata
    29341 acgtgcccat ttgcatatcg attttgtcgc ttaatccaaa atgcttgacg gttcctgcac
    29401 cttttccagt tacgcctgag ccgtgcgcat catcgacgta ggtgatcagt ccatattttt
    29461 caacaatcgg aataatttcc ggcaatcgtg caatgtctcc atccatggag aaaacaccat
    29521 ctgtgatata catgattttt ttatatttct tactttctat cgcttccttt gcttttttct
    29581 caaggtcttt catatcttga tgtttaattc ggatgatttt tgctccggat aagcggcaac
    29641 cgtcaataat agatgcatgg tttaattcat ccgataagat agcatcttct tttgtcatta
    29701 ctgcagagat agcgcccata ttgcagttga atccagattg aaaggcgatt gctgcttctg
    29761 tccctttgaa ttctgcgatt ttttcttcta attgctgatg gattttcagc gaaccattga
    29821 tcgttcggac tgctcctgct cctacaccat aagtttcggt tgcttcaata catgcttttt
    29881 tcaattcttc gcgattggca aatccaagat aattgttgga tgctagattg atcatctttt
    29941 ttccgtctac gatgatacaa gcaccatttt cactttctag tatgtcgatc gtattgtata
    30001 accccttttc tttcagttga tccaattctt tctctaaata cattgttagt tctttggaag
    30061 acattgtctc cacctccata ttcatctgtc ttgcagaaca taccaaacat atatcgtgtc
    30121 attcatctac aaagcaagta tgctatattt ttgataacgc ttacactgat tatgagaatt
    30181 atttaaccac attttttatt tacagtatct ttttactttc aaatgactta tatagtatga
    30241 acaattacct tttagataat gatctatttt actctacctg ggcagataaa tacaacacaa
    30301 ccattgatag aagccctttc gttaaaatca attgtattct gaatatttaa tttatttatt
    30361 gcatagacca ataaaaatac ataaaatttc catcttcgtt tatcagcaat ttttaatttt
    30421 actctctgaa tatttcttaa aagttttact tgttgaattc gaaaaaagga ctgtgacaaa
    30481 agtcttgtca cagtcctatt cttccgaata aacggtgttc tcctgccaat tccgaatcac
    30541 aagttacaat attcatcaaa cacgagaagc tattttttga aattgttcct tgtgactcgg
    30601 agctgaacgg caggctcacg acctcttggt aaacagtgtt ccagaagtaa cttttacgga
    30661 aaatgatccg aaatatcttg gagctgacca cttctgtcac aacctccatc attttttgtt
    30721 atttttgatc caattgtctg gagtaaaatt ttcatcgtca atagaccatt ccagaaaaaa
    30781 ttcttctaag aattcacgca tgaaggtttc tcttcgtttg gcttcttcat atccgtaatc
    30841 agtattcatt ttatcagaca gcaagaacag tttttcataa aaatgattga tcactgtcgt
    30901 gccttttcgg taattttctt tgtccttgaa tgtttgcgga tacagttcag gatcaaaaat
    30961 cgggtgccca tgtccaccgc cgaaataagc agtacgcaaa atgccgattg ctcctaatgc
    31021 ttcgatacga tcagcgtctt gaacgatctt accttccaga gatagttcgg ccgcttcaga
    31081 gagattttta gaaaaggaca tattttcgat aatggaaaaa
//
LOCUS       DAFFSV010000018        30879 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFFSV010000018 DAFFSV010000000
VERSION     DAFFSV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.018"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            79..975
                     /locus_tag="LR110_000756"
     CDS             79..975
                     /locus_tag="LR110_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL4821757.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1125..2066)
                     /locus_tag="LR110_000757"
     CDS             complement(1125..2066)
                     /locus_tag="LR110_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL4821758.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2192..3313
                     /locus_tag="LR110_000758"
     CDS             2192..3313
                     /locus_tag="LR110_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL4821759.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3571..5073
                     /gene="eat(A)"
                     /locus_tag="LR110_000759"
     CDS             3571..5073
                     /gene="eat(A)"
                     /locus_tag="LR110_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL4821760.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5101..5667
                     /locus_tag="LR110_000760"
     CDS             5101..5667
                     /locus_tag="LR110_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL4821761.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5878..6939
                     /locus_tag="LR110_000761"
     CDS             5878..6939
                     /locus_tag="LR110_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL4821762.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6942..7838
                     /gene="murQ"
                     /locus_tag="LR110_000762"
     CDS             6942..7838
                     /gene="murQ"
                     /locus_tag="LR110_000762"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL4821763.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7852..9834
                     /locus_tag="LR110_000763"
     CDS             7852..9834
                     /locus_tag="LR110_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL4821764.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9899..10345)
                     /gene="nrdI"
                     /locus_tag="LR110_000764"
     CDS             complement(9899..10345)
                     /gene="nrdI"
                     /locus_tag="LR110_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL4821765.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10556..11980
                     /locus_tag="LR110_000765"
     CDS             10556..11980
                     /locus_tag="LR110_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4821766.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGKMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12161..12934)
                     /locus_tag="LR110_000766"
     CDS             complement(12161..12934)
                     /locus_tag="LR110_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL4821767.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12931..13836)
                     /locus_tag="LR110_000767"
     CDS             complement(12931..13836)
                     /locus_tag="LR110_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4821768.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13990..14310)
                     /locus_tag="LR110_000768"
     CDS             complement(13990..14310)
                     /locus_tag="LR110_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL4821769.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14784..15470
                     /locus_tag="LR110_000769"
     CDS             14784..15470
                     /locus_tag="LR110_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL4821770.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            15472..16416
                     /locus_tag="LR110_000770"
     CDS             15472..16416
                     /locus_tag="LR110_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBL4821771.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            16615..17259
                     /gene="pcp"
                     /locus_tag="LR110_000771"
     CDS             16615..17259
                     /gene="pcp"
                     /locus_tag="LR110_000771"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBL4821772.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            17338..18615
                     /locus_tag="LR110_000772"
     CDS             17338..18615
                     /locus_tag="LR110_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821773.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(18799..19548)
                     /locus_tag="LR110_000773"
     CDS             complement(18799..19548)
                     /locus_tag="LR110_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBL4821774.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            19877..20257
                     /locus_tag="LR110_000774"
     CDS             19877..20257
                     /locus_tag="LR110_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL4821775.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            20353..20862
                     /locus_tag="LR110_000775"
     CDS             20353..20862
                     /locus_tag="LR110_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821776.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            20939..21118
                     /locus_tag="LR110_000776"
     CDS             20939..21118
                     /locus_tag="LR110_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821777.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            21143..21736
                     /locus_tag="LR110_000777"
     CDS             21143..21736
                     /locus_tag="LR110_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4821778.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(21783..22298)
                     /locus_tag="LR110_000778"
     CDS             complement(21783..22298)
                     /locus_tag="LR110_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821779.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(22324..23193)
                     /locus_tag="LR110_000779"
     CDS             complement(22324..23193)
                     /locus_tag="LR110_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4821780.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            23319..23999
                     /locus_tag="LR110_000780"
     CDS             23319..23999
                     /locus_tag="LR110_000780"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL4821781.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            24152..25132
                     /gene="pta"
                     /locus_tag="LR110_000781"
     CDS             24152..25132
                     /gene="pta"
                     /locus_tag="LR110_000781"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBL4821782.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            25255..25728
                     /gene="tsaE"
                     /locus_tag="LR110_000782"
     CDS             25255..25728
                     /gene="tsaE"
                     /locus_tag="LR110_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBL4821783.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            25721..26245
                     /locus_tag="LR110_000783"
     CDS             25721..26245
                     /locus_tag="LR110_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4821784.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(26735..27268)
                     /locus_tag="LR110_000784"
     CDS             complement(26735..27268)
                     /locus_tag="LR110_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBL4821785.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(28009..28761)
                     /gene="xth"
                     /locus_tag="LR110_000785"
     CDS             complement(28009..28761)
                     /gene="xth"
                     /locus_tag="LR110_000785"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBL4821786.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            28874..29794
                     /locus_tag="LR110_000786"
     CDS             28874..29794
                     /locus_tag="LR110_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL4821787.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            29867..30802
                     /gene="murB"
                     /locus_tag="LR110_000787"
     CDS             29867..30802
                     /gene="murB"
                     /locus_tag="LR110_000787"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBL4821788.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
BASE COUNT        10015 a         5468 c         6465 g         8931 t
ORIGIN      
        1 aggaaacgtt ttactggctt tttactattt aaacaggcta tcataggaaa aagtgacaga
       61 gaaagtgagg cggaaaagat gaacaaaaat gacgtactgc tagttgcaaa aaataaatca
      121 ctttattttt tgccttccat ccgctcttta gatgtttggt ccggaaaaga tttactacag
      181 tcggaattca ttaaatcagc tagacaacag ttggcaaatt atcagctgat cgttttttta
      241 gattatggat ttgatatgga gatggctcga cgcgtgcgcc catttaccaa tgcaaaaatt
      301 gtgttatttt tttggaatca cttcaaagca gaacacgagg cgatgctgaa gcaggcacaa
      361 gatgaaccgg caatcgatga gatttatcat tttgattttt tggaagcaaa agaacttggg
      421 ctgaaacata atagttcatt ttattcgaaa catatgaatt tttcttctag cgaaccaaag
      481 acggatctat tttttggtgc aacgaataat gggagaaaag aacgagcaga aagctataag
      541 caggaattta taaaaagaga tgttacgacc aactatttca ttcttccaca tcgagggaat
      601 gaacaacctg gatatcttac ttatgaagag tacctgaaaa aaacagggga atcgcgcggg
      661 attctggaac tgttgcggga gggtcagcaa ggagtgactt tgcggacatt tgaatccatg
      721 tattttcaaa agaaactagt gacggataat caagcgattg ctcattatca tttttatcgt
      781 cctgaaaata tttttttgct tcgagaacgt gatttagatg aattaccgga atttctgcat
      841 acaccattta agccaatcct atcagagcta ttggaatttt atgatgcaga aaattgggca
      901 aaacgattta tcactaacga ttccactatt tatgagcaat acgaatatga cctctcctct
      961 gttgaaaaca aataaaaaac ctacattttg aagagattat gtactgaaac aaacaagcac
     1021 aattcttatg gcatcatgag agttgtgttt tctttttgat cttttattgg gcttaaaaaa
     1081 agagaaccag ctgctgattc agctgattct catcagagag ccttttattc tgcgccaagg
     1141 attccttctt ttgcatggac taattgggtc aaaaaggcac aatatggtgt agcaatatca
     1201 tatttttgtc cttttcgcca aatggcacca ttgatgtagt cgatttcagt caaacgatgg
     1261 tttttgatca ggtcttggta catagatggg taatgcaacc cgatgttgtc aggatcataa
     1321 gtagaagcaa tatgttggta cacttcttct tgatctagta tgattccttc ttttgctgcg
     1381 atagaagcaa attcagatac gattgttcga accatgctct ctgccgcttt ttgcgcgcct
     1441 agttcagcaa tattacaatc caaaatcgtg cataaaccat tcaatgttcc atttacacaa
     1501 gcttttcgcc aaatggaata acgaacgtta tggctataga tcggatttaa atttgcttct
     1561 tgaaagactt ctacaacttt ttttgtaaat gcttcgcctt ctggttccaa gttctctagt
     1621 tcgatttctc catcacctaa caatgtgact tttccgggac cagctagacc agctgtccac
     1681 atcgtgatcc caaataagat atttttcttt ggcacatatt tttccagtac atcttcatga
     1741 cctaatccat ttagtaaaca caaaacataa gtattgtcag taataatgga ttggatcgaa
     1801 caaaacatat catctaattg attggctttt gttaatgcta cgatcaagtc cacgtgttcg
     1861 ttgctttcga tgatttcttc tggtgaatag attggcagtt ttgcgaccac ttctttaccg
     1921 ttaaagtcag caatcaaccc atttgtacga atggcttcaa tgtgagcagg ccaacgatcg
     1981 atcaagagga cctcatttcc gctttgatgt agcatcaatc cgattcggct gcccattgct
     2041 ccggccccag ctattgcgat tttcatgttt tttctctcct ttattcaatg agttcccttt
     2101 tagtatatag aaaaatggaa gaaaatagca ttgttttgtt tgaaaaaaat gaaaaaaagt
     2161 gtcttactta agaagaaagt gagggataca gatggatatt ttggcggcaa ttgattcatt
     2221 taaaggaagt gcttcttcat ttgaactaaa tcaggcagtt ctaaatggct gggaagatcg
     2281 taaaggaaga aaaatcaatg tgccgattgc agatggcgga gaaaatactg cagaagcaat
     2341 ctattacgcg ctcaggggaa catggcgtac cattgatggg atggatctct tattccgtca
     2401 gcgaaaaatc agctatttat tgacaacttg gcaaggaaaa aacaccgcag tcatcgaatc
     2461 tgcacaagca ttggggttag atctacttga taaaccaacg gatcaaacta tccgacttgc
     2521 ttccagttat gccttaggag aacttattaa agatgcactg cttcaagaag tagaacaaat
     2581 tattgtcact ttaggaggaa gcgggtgtag cgatggtggt ctaggattac tccaatcctt
     2641 gggtgcttct ttagaagggt acacagaagg aaatccttta ttatctacta aaaaacttca
     2701 ttttgatcga agaaacgttc ttgtttctaa agtaaaattg attatcgctg cagatgttac
     2761 caatccatat acaggtcaac aaggagctgc ttggcaattt ggaagacaaa aaggaggaat
     2821 agatacgacg ctagctttct tagataaaca agcaggaaag attgctcagc aaataaaaga
     2881 tttctgggag gtggatttag atgatattcc aggaagtgga gcagcaggcg gtattggtgg
     2941 agcccttttt ttgttgggag cgaagatggt ttctggtttc gatttagtca gccaaatcac
     3001 tggattggaa aaaaagatcg aacaagcaga tgtcatcatt acaggagaag ggcggatcga
     3061 tcagcaaacg atccacggaa aagttccata cggtgtagca gtgcttgcaa aaaaatatcg
     3121 aaaaccaata atcgctattt gcggaagtag agaccaagag ctagaaggac tttctgattt
     3181 tctcccaatt gtcttaagta ttcatttggg tccaataggt ttagaagaag caatggaaca
     3241 caggcaaaca ttagaaaaaa caaaaatctt aggacagaca cttagcagat ttttcaccat
     3301 cctcccgaag tagttgacaa aatcattttc tttatgctat cgtaatttca aatataggac
     3361 aaagtgaggg atccgtgcat tgaaaaatta agtcgattga tgaaagctgc attcagactg
     3421 taccttgtgg agacaaggaa gcagtgtgca gattttcggg attggcttgg ttttttcgat
     3481 gtatgggtct ttttgtgtgt atccaaccct tgcatcctct ttagccagtt ctgaaaatag
     3541 gactggtctt tttttacgag gtgatggaaa atgtctaaaa tcgaaataaa aaatctgaca
     3601 ttcggctacg acagccaagg cacattatta tttgaacaag caaatctaaa ttttgacaca
     3661 caatggaaac taggacttat cggacgaaac ggtcgaggaa agacaacttt actgaatatt
     3721 ctacaaaaca aactacctta tcaagggcaa gtaatccatc agcaagaatt tgcctatttc
     3781 ccgcaacaga caaaagataa agaacgttta acctattacg tgttaaatga tattacggat
     3841 tttgagatat gggaaatcga aagagagctc caattgatgc aaacagatcc tgaaatctta
     3901 tggagagaat tcagcacact atcgggggga gagaagacaa aagtcctact ggcactttta
     3961 tttgtggatg acactcattt cccgttaatc gatgaaccaa cgaatcattt ggatatctct
     4021 ggtagaaaac aagtagcggc ttatttgaaa aagaaaaaac aaggcttcat cgtggtcagc
     4081 catgaccggg gatttatcga tgaagtagtg gaccatgttt tagcaatcga aaaaagtcaa
     4141 ctggaacttt atcaagggaa tttctctatc tatgaagaac agaaaaaact tcgtgatgaa
     4201 tttgaaatgg ctcaaaatga aaaattgaaa aaagaagtca gtaggctaaa gaaaacagca
     4261 gctgaaaaag ccgaatggtc tcgttcccga gaaggagata aaacaaagaa acaagtcgga
     4321 ttcatcgata ctgaatctag acgagtgaat aaaggagcag tgggtgctga tgctgcacgg
     4381 acgatgaaac gatccaaagc aatcgtgaat cggatggaga cccagatcag cgagaaagaa
     4441 aaactattaa aagatatcga atatatcgat tctttgacga tgaatagcca agcgtctcac
     4501 cataagcgac ttttaagcgt agaagatctt caattagggt atgaaaatct gttattcgag
     4561 ccaattcatt ttacaatcga gcctcatcag cgggtggcga tttcaggtcc taacggtgca
     4621 ggaaagtcat ccattatcca ttatcttctg ggggcattca acggcaaggt tataggagaa
     4681 aaataccagc caaaacatct gagcattagt tatgcaagcc aaaattatga agacaatcga
     4741 ggaacgttgg cggaatttgc agagaaaaac caagtagact accaagcatt tttgaacaac
     4801 ctccgaaagc ttgggatgga aagagatgtt tttcataaca agatcgagca gatgagtatg
     4861 ggccaacgga aaaaagtgga attggctaaa tctttatcac agccagctga actatataca
     4921 tgggatgaac cattgaatta tttggatgtc ttcaatcaag aacaattaga acaactgatc
     4981 ttgaacgtga aacctgccat gttactagtg gaacatgatc aaaccttcct ggataaagta
     5041 tctactgaga ttatttctct tgagagaatc taacccttgt aatctcaaga acaacaaggt
     5101 atggttaagg ggaactttgc caacgaagga gagaatcaca tgctttatgt aacgagacac
     5161 ggagaaacaa cttggaacgc acaaggatta gtttgcggac gggcagatgt gccgctgact
     5221 gaaaaaggac agatgcaggc acagaaacta gcagaaaagg tagtagattt gcctgttcca
     5281 atcacgaaaa tcatccattc ccctcttcaa cgagcaagag atacagcaca agcagttgct
     5341 gatcgtctca gcttgccgct gaccgttgat gaacgattgg tagaaatgga ttttggggat
     5401 tatgatggac tgccaagcaa agatgagaac tttcaaaaag cacgtttagc gttcgctgta
     5461 cgattcccaa atggggagtc agtcttagat gtgtatgcac ggatcgttcc tttattgaaa
     5521 gaatgcatcg aagacgaaga aaatgtctat ctgttagttt gccataatgc cttgataaga
     5581 gtgatcaatg cttattttca tccgatgcct aatgaggggt tctttacatt catggtagat
     5641 aatacagaat tggtttctta tgagtaatta tgtttttgtg ttatttaaat aaaatatttc
     5701 aaggcactgc ttttgcttag cagtgccttt tttaggttat gacaattctt ttgataatat
     5761 aaactcaacc tttagaaatg aaatttaatt attttaataa aagtattgaa attttgtttc
     5821 tttagatata tactctagtc atagaaatga agacggatac agaaaaagag ggataatatg
     5881 tttggctttt caatatttat gaacgattct ttgtcagaag agaagaaaca gtatatccgc
     5941 caaatggcat ccaatggctt tgtcggtatt ttcacttcca tgcatatacc agaagatgat
     6001 gtttcggcat atcaaaagcg attgatcgaa ctaggaagcg aagcgaaaaa gaataaacta
     6061 gaattgatgg tcgatatttc aggagaggca ttggaacgag cgggtttttc attagaagat
     6121 ctaacgcctt tgaaagcaat tggtgtaaca ggtttaagaa tggattatca tatttccaat
     6181 cagcagattg ccgactggtc acatcaattg aaaatcagtt tgaatgctag cacgatcacg
     6241 cctaaagata tcgatgaatt gaaagaagca gaagctgatt tctcacagat tgaagcttgg
     6301 cataattatt atccacgtcc cgaaacaggg ctggacaaag aatggtatca gaaaaaaaat
     6361 caatggctca aaattcaagg tctccttgtc caaggttttg taccaggaga tacagagttg
     6421 agaggcccac tatatcaagg attgccaact ttggaagaac atcgagggat ccatcctctt
     6481 gcaggcgctc tagatctatt agcaagtaac acggatattg tttacatcgg agatgcaggt
     6541 ctgtcagaaa acgttcagga gcaatttgct tctttccaaa aagaacagac agtcttgctc
     6601 catacggaac ctgtagatga agaattttac gaatatatat tagggaagca tacgaatcgg
     6661 caagacgatg caagagatgt catacgaagc gtagatgcac gttttcgaga gattccgcct
     6721 atcccagcaa gaaatacagc tacgcgtatg aaagggtcta ttactttaga taacgaaaag
     6781 tatttgcgct atatgggtga gatccaatta accaaatatg atttgccagc ggatgaaaaa
     6841 gtgaatgtag tagctaaagt tatcaaagaa gagctaccat tgatcaacca aatcaaagct
     6901 ggtatgaact atcaatttat aagaaaagaa ggaagataaa aatgagcaaa atcaatttag
     6961 agaacctaac aacggaacgc agaaaccaag aaacttttgg attagatgag atgagtattt
     7021 ctcaagccgt tcaactaatg aacaaagaag atcagaaagt ggccgctgcg gttgaaaaag
     7081 aactgacaag tatcgaacca gtgattgcta acacgattga gtcctttaaa aaaggtggac
     7141 gtctgattta tatgggagcc ggaacaagtg gaagactagg tgtgttagat gctgctgaat
     7201 gcgtaccgac attcggtgta gaaccggaaa tggtcatagg tctaattgct ggtggagaag
     7261 aagcaatgac tgtagctgtc gaaggggccg aagattctta tgagctaggg caagaagatc
     7321 taatcaagct gaatttgaca aaaaatgata tagttattgg tatcgcagct agcggtcgta
     7381 caccatatgt gatcggtgga ttagattacg caaaatcgat cggtgctgtc acaggaacga
     7441 tttcttgcaa taaacaagcc gaaatcagta aacacgcgga tttcccaatc gaagtcgatg
     7501 caggtccaga atttttgaca ggctctactc gattgaagtc aggaacagca caaaaactta
     7561 tcttgaatat gatttctact atcagtatga ttggtatagg taaagtattc aataacttga
     7621 tggtcgatgt caaaccaaca aatgaaaaac taatcgaacg ctcaaaacga atcatcatgc
     7681 aagcgacaga agctgattac gaaacagctg aaaagtactt tgaagaagca gatcaaaatg
     7741 ttaagctggc tatcgtcatg attttgacaa atagtgataa agaggaagct caagcaaaat
     7801 tagctgaagc aaacggattt gtaaaaggga ccttatgagg gggaaaagac catggcaaaa
     7861 aacgaactga ctttggaaga actggcaaaa caaatttata aaggggcagg cggcatgggc
     7921 aatgtagaaa gcattgtcca ctgcatgact cgtgtgcgta tgaagatcat caatgatgat
     7981 cttgtaaatg tctctgaatt aaaagcgatt ccaggagtgc ttggtgtagt agaagatgaa
     8041 cagcttcaag tcatcatcgg acctgggaaa gtgaacaaag tagcacagca aatggttgat
     8101 ttggcaggcg tgaaattagg agaaaaactt tcttctgtaa gtacagctac agcaaattca
     8161 tcagcatctg gaaaagaccg tgtaaacgaa cgggcacaag aaatgaaagc ggcacaaaaa
     8221 tcgaaacaaa aaccatctaa aatcaaatcg gtattaaaag acatttctaa tatctttgtg
     8281 ccaatgattc ctgcatttgt cggtactggg atcgtggcag ggattgccgc agttttagca
     8341 aacttagtca cagccggaac acttgatgca gcaacatggc aacaatatat cgatgtgatg
     8401 aacatcttga aaaacgggat gtttagctac ctagttatct atacaggtat caatgctgca
     8461 caagtttttg gtgcaacacc aactcttggt ggagttatcg gggccgttgt gatgctgaca
     8521 gggatgaatc cggaagcacc actgaaaaac ttatttactg gtgaagctct tgcagctgga
     8581 caaggtggga tcctaggtgt catcttcgct gtttggctat tgtcaatcgt tgagaaaaaa
     8641 ctgcataaag tagtacctga tgcggtagat atcatcgtta caccaactct ttcattgatt
     8701 gccatcggtc tgattgaaat tttcttcatc atgcctttag caggtttcat ttctgatggc
     8761 atggtcggtg ggatcaactg ggtacttggc gttggtggag catttgccgg cttcgttcta
     8821 ggcacattat tcttgccgat ggttatgttc ggactgcatc aaattttgac tccaatccat
     8881 gtgcaaatga ttgatgaaac tggtcgtacg ctattgcttc caattcttgc gatggctggt
     8941 gcaggacaag tgggtgcagc attggcatta tggattcgct gcaaaaaaga taaagaatta
     9001 acagaaatga tcaaaggagc tttaccagtc gggatcctag gaatcggcga accattgatt
     9061 tacggagtaa cattacctct aggacgccca tttattactg cttgtatcgg tggaggtatc
     9121 ggcggggcag tgatcggaat gatcggaaat gtcggggcta tcgctattgg accttcagga
     9181 gccgctttga tcccattgat ctctgacggt aaatggttag gctacatcct tggattattg
     9241 gcagcttatg ctggtggctt cgttgctaca ttcttcttcg gtattcctaa agaccgttta
     9301 gaaaaagaag aattagcgac tgaaacagcc acagtaaatg aagtgcctga aaaagtagta
     9361 cctcaaactt cagctgaaag tgtgactatc ctctcatcag ttgctgacgg aacagttgtt
     9421 tctttagata aagcaagtga tccagttttt gcacaaaaga tgatgggtga aggctacttt
     9481 gtagaaccag aaaacggaca tatctattct ccagttactg gggtagtcag ctctgtcttc
     9541 ccaacgaaac acgctattgg gatcacaacg ccaaatggtt tagaaatctt gcttcatatg
     9601 ggaatcaaca cagttgactt aggcggtacg ccatttgatg tgaaagtaac agaagggcag
     9661 caagtgacac ctgatacgct tgtagcagaa gctgacttag aagcgatcaa agcagcaggc
     9721 aaagaaacat ccatgatcgt cttggtaacc aatatggatc gtgtaaaaaa ctttgtcttg
     9781 gaaaaaacag gtaaagcaaa agcaaaagca cctgtgatgg atgtcaaagc ttaatatgat
     9841 agaaacaata gaaacgaaag aagaaactga aatctatttt tcagcttctt cttttttatt
     9901 atttattgtt ttctgctaaa atctgataaa ttcgtgtcac atcagatggt gttccccgta
     9961 attcataatc tgccaagaaa ggaaagccaa atgtttgtgc atattgtttg gctgttagac
    10021 aatactgatt attgaagttt ttatttccgc taccgactac accaagacaa agtttatgat
    10081 tgtcttcatg ttccagatat tcacgcatcg tttcggtcaa gatttctgta tcaccgttat
    10141 ctaaaccatt tccgccgtct aaataagtag gaacgaaagt aaaaaatggt tctgtttctt
    10201 tcgtaaaatc actattttca tggatttctt ttaaagtaac ttcaggattg acaggatctt
    10261 ctgcatgcat tttttcagca tattctgcta gatgtttggc aaaggcacgc gtatttcctg
    10321 aaatcgatat atataaaatg ttcatggatc atcctccaat tctttccttt tagtatagca
    10381 tcttttttaa gttttttggt ggtgtaattt ggaaagggac taggcgcttt gcttgtataa
    10441 tgagaaaaaa gccggtagaa ttatatttct attaaagttt ttattaatta agtaaaaata
    10501 aaaaaggttt gttatacttt ttgataaaag cggttttatt ttggaggatg cttacatgga
    10561 tacagagcaa cagaaaaaag gaattggttt aattccactt gctgccttag taattggttc
    10621 agcgattgga ggtggcgtat ttggtattat gacggacatc tccggatcgg ctggcggccc
    10681 agctattttc agttgggtac tcgtaggaac agcaatgttg atgctctcct tgtcgatcaa
    10741 taatatcaat aaaaaacgtc cagatttaaa aggcgggatc tttagttacg cagaagaagg
    10801 ttttggccgt ttcgctggtt ttatcagcgg ttggggttac tggctcacat gctggttagg
    10861 caacgtagct tttgcaacat tgttgatgag cgcattagga tatttcttcc cagtatttga
    10921 aggtgggcaa aatctggcat cgatcgtgat gagtacagtc tttttatggg gatatgcatg
    10981 gctagtgaat cggggagtcg aaaatgctag tttcgtcaat gctatcgtca cattctgtaa
    11041 attagtgcca ttatttttat ttattgtagt agctatcagc acgttttcga tccaacaatt
    11101 cattgataac ttttctagcg acattatatt gaatgcgaat ggacagccga tcccgttttc
    11161 cagtcaagta ttgagttgca tcatggtgat gctttgggta tttgttggta ttgaaggagc
    11221 ctctgtcgta ggttcacgag caaaaaaaca atcagatgtt gggaaagcaa cgattctagg
    11281 gatattcggc ttgattatta tttacgtctt ggtttccatg cttccatatg ggaaaatgac
    11341 gatgaatcag ctgcaaacga tcaaggcgca gcctgctatg ggctatgtgt ttgaaatgat
    11401 ggttggtaaa tggggagcag tcgtgatcaa cggcggtttg atcatttctt tagttggtgt
    11461 ttggctctca tggacgatct taccgattga aacaatggga aacatggctg aagacggact
    11521 tttgcctaaa gcatggggaa aactcaacaa aaatggggca ccaacttatg ctatcgtatt
    11581 gacgacgtta tgtacgaatg tctttttact ttcattatta ttcactgaca gtgcttataa
    11641 ctttgcttac acacttggta cagcagctat tttcttcacg tggcttttct taggattgta
    11701 ccaaatgaaa ctttcttatc agcggaaaga atgggtccaa tttttagtag ggcttttagc
    11761 aagtgccttt caagtatggg ctatgctagt cgtagccttt aatgaagtaa tgcttgttct
    11821 tgttcttttc ttgccaggca tttattttta cctcaaagca cgtaaggagc aaaacgagcc
    11881 tgtacaacat atccacttag ataagatttt acttataggt gtgacgatgg tcggagtgat
    11941 tgcaatggtt ttatttgcca ctgggatgct tcacgtatag aagtataaaa gaagtataaa
    12001 gagaggttgt gagcctgtcg tttattcaaa aacatactag gaaaggagct gtgacacaaa
    12061 tgttgtcaca gctccttttt cagaataaac ggtgtttaag agacagccaa tcggcaataa
    12121 ggctgaacgt ctctttcaca gcctcttttt tgatgagtca tcatacattt tttcgcttaa
    12181 atacgagata tcctaatgct aagaacaaaa cgatgtaagc taaattaccg aatagcattt
    12241 gattagtaga aagatgagtc aattgcttca tgatctcttc gtttccaatt tggttttgca
    12301 gattcaacat actgatcgga ttccatttga tccactccca tttttggatg agtgcaataa
    12361 ggataccaga tatgattgac gaagcaaaat aaaagacgat tcctgctgag atggatgcgc
    12421 cggaactatt gataaagcaa gcaatcatca acactaaact taaaatcagc cataaaccga
    12481 tataactacc caaagtataa gtgaacaagc tttggatcag tgtttgacca gtagaaacct
    12541 tctccgtgaa ggaaatcgaa gggaaaagaa tcagtttcat caacacagtg acaataatag
    12601 ctaaaaggta taaataaaca gaataaataa ctaatgtgat ccatttgctt actaatattt
    12661 ctcctcgaga atactttcga taaaggagat ttttcaatgt accatattgt gcttccatcg
    12721 aaatgatcgt gcttgcagct gcaatcatga taaagacaat ccaagatgtg gcactaaaaa
    12781 gctgagcagt catttcaact ggatcaatga tcgtcgtgta ttttttggct agaagtgctg
    12841 ttcctatgag caaaacaact aatagaacag ataaaacagc agttgacttt ttcttgatca
    12901 atttaaataa ttcttgtttt acaagcaatg tcatttttct tcttccccct ttactgaatc
    12961 cagaatttcc aatagagaag attccaaatc attcgtcaga tgctggacgt ctgtcacatg
    13021 gatattttgt gcagacaata gctggatcac ggttcctaat aattcttcgt tttcttttaa
    13081 aagcttgatt ttatcaggat cttccaaagt atatccagct tctgtcaaga tcgttcgagc
    13141 agctagatca tttgttgtag agataacgat gaaatcttgc gctgtttggt tcaattcagc
    13201 cattgtacat tgtttgatga tctttccttg attgatcagg atcagctcat cgaccatttt
    13261 ttcaagctcg ctgagtaagt gactggagat caagaaagag gtgccctgag tagccaagtc
    13321 ggtaatgatt tttcgcacat ctcttgttgc ttgtggatcc aaaccgttca tcggctcatc
    13381 taaaataacg atagacggat ggttcagtaa ggcaagagca attcccaatt tttgtttcat
    13441 tcccaacgag taggatttcg cttttttgtg aatgtacttt tccatcttga gctgattcac
    13501 gatagtgtcg atagcagata catcgttggt ttcagagaat aacttcagat gatcatagcc
    13561 tgttaagaat ggatagatac ccgggtattc gatcaatgct cctacttgtt ccaacccttt
    13621 atgactagag ggagaaatct cattttcacc aatacagatt gttccatctg agtaatggat
    13681 cagtcctaaa atagctttca tgatcgttgt ttttccagcg ccattaggac ctactaatcc
    13741 aacgattttg ccaggggata agctaaaact gacatcgctg agggcttgtt tgtgaccaaa
    13801 gttcttggat aattcttgga ccgaaagact tttcaaaaat attcactcct tttctataga
    13861 tagtacaagc atacacctga tgagtggaga agtcgctagg tcattggact tatttcttat
    13921 tttttcataa aaaaattgaa tattaaataa aaaggcttat tttttctgat cagtacttat
    13981 tccaagtgtc tagtggccgc tagtaatctt gatgccaatg atactgatga gtagcatccc
    14041 cacaaagaac caagtggaaa gattcagctt atcatgaaac aagaccacac caattatgac
    14101 cgaaccaact gcgccaatcc ctgtccagat aggataagag agactcatcg gcaaactttt
    14161 gattgccttt gacaaaaagt aaaaactagc aatcatgcca atcactgtgt aaaaagaata
    14221 attgatttta gaaaaaccat cactccattt catcatcgtt gaccagaata cttccaaaat
    14281 tcctgctatg attaattcca tccatgccat acttacattc ctccttaact ataaaaaaat
    14341 acacaaaaag aggaaaatca tcccttcaaa gacctaatgg aatgatcatc ctctttatcc
    14401 tacccggtgg cagattgtat atctttttct gtagtatagc acacctatcc agatatcggc
    14461 aatctcatac tttttagtaa agtgtaccaa gcactgacgg atatatcgtt agcggaaaag
    14521 aagatgaatg ctttgcaatt tcatagaaag aagttgttat ttattaatca tttaaaaaaa
    14581 acagtaacta gaccttattt ctatatatta tcgggcaata aattaaaaaa agctttacct
    14641 tacaataagt aagataattt ttgtgataaa aaaataacga aaaatattta aaaatagtgt
    14701 ctatttcgtg agaaaacaac tatatttttt atgtgttttg ctttataatt aaaaaaatat
    14761 tgtcatttta tggaggaaaa tatatggaaa attatcttag ccttttaggc gtcttgattg
    14821 ttattctcgg gtttgcattt aaattagact cgatattgat cgtaatggtt gctttagttg
    14881 ttacagcttt gactggcggt cttggcattc aaggaatgtt agaactttta ggtacaagtt
    14941 ttgtaaacaa tcgcgggatg gcgattttta tcatcattat gttggcaaca ggaacgcttg
    15001 aaagaaatgg tctgaaagaa tcagcggctt ccttgatcaa gcgattcaag aaagtatctg
    15061 ctggtatgat tgtagatatt tatgggatct ttcgaatgat ctttgcggct tttaacgtga
    15121 gttttggtgg agttgctgga tttgttcgtc caatcatttt accgatggca ttggggacca
    15181 tcgaatcaaa agggttagaa gtccatccta agcatgaaga agaactaaaa ggtatggctt
    15241 cagctatgga aaatgtctgc tggttctttg gccaagtact attcatcgga ggagcagggg
    15301 cgttgctagt ccaatcaaca ctgaaagatc taggatatga cgtaacattg ggacagcttg
    15361 ctttagtgga agttccagtg gctatcacgg ctttagttgt ggcaagtgtc tacttctatc
    15421 ttaaagaaaa gaaattagcg aagaaatatt atccaggaaa ggggcaataa gatggacttt
    15481 tttacaaatc ctgatatttt attaggcgat aaattactgg agatccttta tatcgtgatg
    15541 ggactcgtat tgatttacac tggagtaaga aatttaacag ataaaaccaa tcctcatcgt
    15601 tatgggtcag cctttttctg gacggtctta ggggtagtta ttgcaggagg aagatggctt
    15661 ccggcaatcg tgaacggtgg cttgatcttt gcgatgacca taccagcaat tgctaagaaa
    15721 gtcagcaaag gagaaagccg gcttccatca aaagaatata tggaaaaaat gtcagacaaa
    15781 ctaggaatga aaatttttat tccagctttg agtatcggtg tctttgcgat cttgtttgct
    15841 ctatttacca atttaggtgc tttagtcggc gtgggcgtcg gtgtttttgc agccatgcta
    15901 atcttgatgt tcttttctcg agataatcga ccaagtacat ttttagatga tgcagcagat
    15961 atgctgggca cagtagggcc gttaagcatg ttgccaatgc ttttagcttc tctaggtgcg
    16021 gtatttacaa gcgctggagt tgggacagtg atttccaatg cagtaggtac gatcgttcca
    16081 gaaggaaatg tgaacatcgg catcatcatt tacgctcttg gcatggtgat atttacggta
    16141 atcatgggga atgcatttgc ggccatcacc gtgatgacgg ttgggatcgg tgcaccattc
    16201 gtatttagtc atggagcgaa tccagcgtta ataggaatgg ttgcattgac ttgcggtttt
    16261 tgtgggaccc tgctgacacc gatggctgct aacttcaata tcgtgcctgt agcgatgctg
    16321 gagatgaaag acaaatacgg agtgatcaaa aaccagttgt ttattgcgtt atttatgcta
    16381 gttttccaaa ttgcttatat gattctattt aaataataga agagaggggt tgtgacagaa
    16441 taattgtcgc aacctcttct aacattgtag tggaacacta tttttttata agaatatatt
    16501 aagtttgatc tgttatgtta tttaaactat tgtttccttt atttacgctg ttcaaggaaa
    16561 atggtatgat aaaagataac catatgttaa atcaatcgga cggaggaaca acagatgaaa
    16621 gtactagtaa caggttttga tccatttggt ggggacaaag tgaatccagc ctatgaagca
    16681 gtaaaaaaaa tgccagatga aatcagcggt gctgaaatca tcaaagtgga agtaccgacc
    16741 gtattcgaaa aaagcagtca agtagtcaaa gaagccatcc aacagcacca accagatgtc
    16801 gtgatctgtg tcggacaagc aggtgggcgg tcagcagtct cgttcgaacg tgtcgcaatc
    16861 aatctagcag aagcaagaat ccctgataat gaaggaaatc agccattcga tacggcttta
    16921 gaagaaaacg gtccagccgc ttactttacc tcattgccaa tcaaagcaat gacaaaaaat
    16981 gtacaagatc atggtctacc agcgtacatc tcttatacag caggaacatt tgtctgcaat
    17041 gatatcatgt atcgtttgct tcatttgatc gaaacacaat tcccggcaat tcgaggtggg
    17101 ttcatccacg tgccattctc acccgatcaa gtgatcgatc gtccagtggg tacgccttct
    17161 atgtctttag aagacattgc ttcttctttg acttatgcag ttgaagcagc agtagaaaat
    17221 aaagaagata tttccggaaa tgccggaaca actcactaat ttagcaagaa acactggctg
    17281 gttgctaacg ttatgttagt agccagcttt ttgtaattag aaaggaaaga aacagccgtg
    17341 gaagaatggc aatccgtctt tgaagaatgg tttccaaaag aaatcagcaa aagctatccc
    17401 atcaaaattt ctaaacaata cacaagcagt caacgttggg agatctatgc aaaattaacg
    17461 aaaaaacagc gggaactagt cgataagcac cgccgatatc taatcagctc tcgttttatg
    17521 gaagaacatt atttagctgc aacagattgg gtgttttctg acttcaagat caatccgttt
    17581 tttcgaacaa aacgaagcca gcaaaaattg tattgtgagt gtggtagaga attaaaagtg
    17641 caatacatcg ttaaatcgcc aaaaaccgga aagatcctaa aactaggcat caaccacttt
    17701 gccgatcacc tgcatgtctc tccgacagtg gcagcttcga tccatcaggg aatgacaaaa
    17761 gttgatctgg ccttagacga attattatgg cttaagcaga agaatatcga ttttcctgaa
    17821 ggactatggc aaaaatactg ttttgtcttg taccaaaatc gtcggatgaa acagccatat
    17881 ttaccggata taaaattagc tcagcggctc gcagaatttc gccaagtaga aatgccgatt
    17941 tacatagcgg attatcaagc attggaaaat gaaatcaaga aaataagtga acacatcaat
    18001 gggcagtcaa aaaagcgaca gataaaaaaa gaactatttg acgactttgc agaagaatta
    18061 gtaaaggatg tagaagaatt cttgatcaac tatcgcgcat ttttacgtaa agattggcaa
    18121 tccatcgtat atgaagaagt gccagtacat cctaatgctt attttgaaac atttatttcc
    18181 gtgttgagaa agacaaaacg tcaacgaaca ccagaagtca ctgctcagat ggaatatttt
    18241 gcgaaaaacc aacgattcat ccaaccaaaa atctatctat ttatttggaa gcaatattgt
    18301 cgctatggtt ttacagaagg gttcttcgac agcattcctc ggatagtcag aaatggtttt
    18361 ttaaaagtat tgcgtaaaga acgagaagct atacaatctg ctgataagaa agaccgtaca
    18421 gtctcaaaag aaaaatggca actagtagtg aaagatatcc aatctggaaa tgtgcaagag
    18481 acaatcgaca aatggaaagg aaaacattat cgttttactg aagctcagaa acaagcgttg
    18541 gaatattatc agaagttaga agaatcactg cgtttcaatg atgaggcaag aaagtatctg
    18601 aaggaattac tataaaaaat agaggctggg acagaagcgt ttaactccaa gaaataagaa
    18661 gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt
    18721 gcttctgatt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt
    18781 gtcccacgct cagttttttt atgcgtgttg ttgtgcgttg tagtattctt cttccatttt
    18841 ttgcatacgt tcacgttctt tttggttata ccaaggtgaa acggtaaatg caaggacatc
    18901 attcagcaag taaacagagc tgttcacgaa catcgccaaa cttgcagatc catgcgtaaa
    18961 tgaaatatac caaaggacca tttgagcaag ccctgaagct aaccaccagt aatattggtt
    19021 gttgtaacgc aagaaactga tgataccagc agtcaagcta atagaaaaag caatggcatc
    19081 tacccacggc cgaggatcat ctgtataacg accaatcagg aatcctgaaa caagatagac
    19141 aaggaaagta ctgatgattg cgataaacca gctttttcca gtgaattttc ggatttttga
    19201 agccatatta tgattccatt ctttacttaa caatacgggg atatccaatg tgatcatata
    19261 agcaatctgt tcaccgatac ttaaatagtt ctttgctgaa aacccaacga taataaaaca
    19321 aatcgcagac aaaataccta aaataccgtt gactgatttt gccgcattga tggataaaat
    19381 acacaataca ccaagcgtgg taccgataaa tgtaataacg gacagccaag tgatgggact
    19441 attgacaaga gtcatgacct ggcagccaag actgaaaaag aaaagataat aattttgttg
    19501 cggccagcct tttagctgat gagctaaaaa ctgaaaatat gacttcatgt atgaggactc
    19561 cttaagacac taaatgtagt gtgttctgta tttactaaca caatctattg tatgcttttc
    19621 ttgaaaaaaa actagtcttt caattataaa taatttgtgt tatgataatt ttctgtttgt
    19681 tttcttgaaa atataatttc ttactttcat ttattatatt gggaacagga agagtgtctt
    19741 ggaggaccta ctttctgttt ttttgccttt tttcttataa aaaggaaaaa cagctatttt
    19801 tttcaatctg acacatttac aatggaactg gagattaata tagatccgag cttaaacgaa
    19861 gaataggagt gctcatatga accttacaac gatccatcat atagccatca ttgtttcaga
    19921 ttatcagaaa tcacgtgatt tttatgttaa taaattagga tttgaagtaa tcagagaaaa
    19981 ttaccgcaaa gaacgagatg actataaatt agatctgaaa ctaggaagtg ctgaattgga
    20041 gatttttgga attgctaatg cgccacaacg accaaactat ccagaagctt gtggcttgcg
    20101 tcatcttgct ttcaaagtag acgatattga agaaacaatt gcagaattgc agtccttagg
    20161 gatcgaatca gaaccgatta gagaagatac ctttacagga aaaaaaatga cgtttttctt
    20221 tgatccagac ggattgccat tagaactgca cgaataaaga taacaaaatt gtcacttttt
    20281 tttcgtttca ttcgattttt cattcgttca tttcttgata ttctttaaaa aaaagaaaca
    20341 ggagaatgaa gaatgaaaaa aatgatgttt ggtgttgctg ccctcatcgt aatagggatt
    20401 accggtatga cgggactgct caatgccgct tttcacatgg atgatattag tgaccaggaa
    20461 tatcggatct cattgaaaaa agcagcagca atcgtaaaaa acgaaacaaa agaacataac
    20521 atatctgcta ttcattttgc agttgaaaaa gatttggaga atcctacgaa tgcaacatat
    20581 gaataccagt ttttttgtga aggacaagtc atagcagtca atccatccac tgggaaaaca
    20641 accgtacgaa atgacacgga aaaggccgca gaacatcttt ttgatataga aaaaacggaa
    20701 gagttcaaac aaccgcaagc agcgatgaag gaagcagtca gctactacgg gaaaaaagca
    20761 aaagtgaaag agtggacact tctagtcaaa gatgaacgtc cttattataa agtccgatta
    20821 atagatggag aacaggaccg tcaggtatgg atatctgcat agaagaagga tattgcttct
    20881 cttttttaca atataagagt aagctaagag agtaaagatg aaaggaagtt gagataacat
    20941 ggaaaaaatg aaatgcccta attgcggaaa aaaatttgca tacgaagaag tcaataatgt
    21001 agtcgaacat aatgataaag aaatgccagt cgtctgccct tattgccgaa ctgaagcggc
    21061 aagaatcgtt actcatggtt atttcattac tgaaaagatt gaagattttt taaaataacg
    21121 attttgcgat ttctttttct gcatgttaga atggagaaaa ggagaggaaa ctatgattcg
    21181 ttttgcgaga aaagaagatg ctccagaaat cgccccattg atcttagtga ttctaaaaga
    21241 tatggaactg ccatttttga agcagacagg ggaacaaaag actttagaga ttttagaaga
    21301 agcaatcact gatccaaatt atcgttacag ttattcacga ggtatcgtag aagaaataga
    21361 cggtaaagtt gcaggagttg cttacggtta tacagatgaa gaagaatcaa tcatcgatca
    21421 gccactggta cctatcttag agaaatatca gctagatgcg tctacacgtt tgttcattga
    21481 taaagaaacg tttcccaacg aatggtattt ggactctatc agtgtatcag aagattttag
    21541 aggacaaggc atcggttcgc gattattaga agcattacct aaattggcga agaaagctaa
    21601 tcgctccgtc atcggtctaa gtgtggatga aaaaaatcca aaggctaaaa aattatacga
    21661 acgccatgac ttcaaagttg tcggtcagcg gatgatcagt ggtcatcttt atgatcatat
    21721 gcaaaagaat atctaaaaag atgtacctga aatgcgtatt tccgcatttc aggtacatct
    21781 ttttaactta agttaagtcg gtaagaaaca ggcttatcgc gacttttgtc tttacggtag
    21841 agccagtaat ctaaaaccgc agcaatacaa atacaaactg gagcatcttc atcatttgag
    21901 acatccaata catagtaatc tcctgaaaaa agtgttgctt tatccatttt catgatttgc
    21961 tgattgaaat gatggatttg atattgatga ttatagatat caccttggac tgtccagtga
    22021 agtctacgaa tataataaaa gtcaccgggc caattgaata ttttctgcat ggttcccacc
    22081 ttttcgaact ctttatacag ctcaaaacgc gtaccgaatg tcaggcttat ctgcttgatg
    22141 tgagcaacga gatcgccatt catggcataa agagacaatg catcgccctt tgttccccag
    22201 cgaccaacca tcaaaaacag ggaatgtcct ttttcgtttt tgacaattgt ccgagttgca
    22261 cgtccaagct gcttttcctg aatgaaaaat tctgacatct atatccctct tttctagata
    22321 aaactacaag tcttctgcaa tcgcgcgcaa tatggctttt cctacgccag attcaaggtt
    22381 tgtgtccgtt gtatatttgg cataatcttt tacttcgatt tctgcatttc ccattgcgaa
    22441 actgactcct gctacttgca gcatgctgac atcattgaag ttatcgccaa tcgtcatgac
    22501 ttcttctaaa gaaatcccac gctcgtgagc gacatgggca acagcaattc ctttttgagc
    22561 gtttttatgg ttcacttcga tattgttttg accagaggaa gtaacggcta gatcgcctaa
    22621 aagagaaatc tgctttccaa ctggacctaa tacagctggc ccttctgtat gaaaacaaat
    22681 aatttttaaa acttccagat tcttttcttc caaaatagca cgaatactgt ccacgtatgt
    22741 gatgtgcaat aactccagct tagctgaggc catggcaatc gccattttat acgtaaggtg
    22801 aggtaaatgg gtagccacca tggaagcaaa actttcaatt cgtttcgctt gattttcaga
    22861 ataaagacca tcgttggttg ctacttcata gtaaataccg ttagcatcta gaatatctaa
    22921 aacagagatt gcttgattat ttggaatcga cacggtaaac aatgaatttc cttctttatc
    22981 aaatacctgt gccccgttca aagtgatcat cgcacaatcg attcctacct catctaaagc
    23041 tgcgcgagct tcttgatagt ttcgaccagt agcgaccatg aactcaacac cggcatcatt
    23101 tgcaaaacgt atcgcctcgg cattttcagg agaaacactc atatgggcat ctaataatgt
    23161 gccgtccata tctgatgcaa ttaatttgat cataatgatt atcgttcctt tctgcttctt
    23221 gttcctagtt taccatatta atcaagtgct gagggtgaag attgtccctt gtgattgaag
    23281 aaaatgtgag ttattctttt tattaggagg tggagttttt gaaagaaatc attcataaca
    23341 gttggcaaga agttttgtcc tcagaatttt caaaagatta ttatctgcat ctaagagaat
    23401 ttttaaaaaa agaatatgcg tcccaaaaga tccatccaga tatgtatcat atctatgaag
    23461 cattggaatt gactccttat gaagaagtta aagtggtgat tttaggacaa gatccttatc
    23521 atggagaaaa tcaagctcat ggcttatctt tttctgtgca acctggcgta aagatcccgc
    23581 catcgttaag aaatatctat aaagagcttt atgatgatct tggaatcgca cctgttcaac
    23641 atggaaatct agtgtcatgg gctaaacagg gagtcctttt actcaacacc gttcttaccg
    23701 ttcgggaagg gcaagcgtat tctcatcgcg ggaaaggttg ggaaagatta acagacacga
    23761 tcattgaaaa attaaatgaa cgggaaaaac caatcgtgtt tatcttgtgg ggaaaaccgg
    23821 cacaagaaaa aatcaaaatg atcgataaga gtcgccatat catcataaca tctccacatc
    23881 ccagtccgtt atctgcaagt cgtggctttt ttggttccaa acccttctcg aaaacgaatg
    23941 atgcgctcct tgcgttagga gaagaaccga ttgattggca actgcctgaa acagtgtaat
    24001 aaaacagcgc tttcctgtta tagtgcagtt cgctttgtat gtgaaaaact taatttttcc
    24061 agcatttttg actgatacag ataaaataaa ctgttctttt tttctggaat agtgtatgat
    24121 aatacatata aaaacgtctg gaggtcatct tgtggaattg tttgatagtt taagatttaa
    24181 aattgttcgc cgtgggatca aaatcgtttt cccggaagct actgatgctc gtattttagg
    24241 cgcagctgcc cggttgaaag cagaagaact tgtggaaccc attttagtag ggaacgaaga
    24301 agaaataaga aatgcagcgc atgccagagg aatcaaaaca tctagcttta cgatcatcag
    24361 ccctgataat tacgataagt gggatgaaat ggtggatact tttgttgaac gtagaaatgg
    24421 aaaggcatcc aaagaacaag cagaaaagat tttaaaagat gtgaattact ttggtacgat
    24481 gcttacgtat atggggattg cagacggaat ggtctctgga gcgatccatt cgactggcga
    24541 cactgttcgc ccagcacttc agatcatcaa aacaaagcct ggcatcagtc ggacaagcgg
    24601 agcgatgatc atggtacgtg gaagagatca agaaaaatat gtcttttcgg attgtgcgat
    24661 caacgtgaat ccaactgcgc aagaattggc tgaaatcgcc gtagattctg caaaaacagc
    24721 agaattgttc gatatcgaac caaaagtagc aatgatgagc ttctcgacaa aaggctcagc
    24781 gaaatcgcca gaagtagaca aagtagttga agcaactaaa atcgctaaaa aactagcgcc
    24841 acaatacatg atcgacggcg aattgcaatt tgatgcttct tatgttcctt cagttgcaca
    24901 actgaaagcg ccagattcag aagtagctgg tcaagcaaca gtctttgtat ttccagaatt
    24961 gcagtcagga aatatcggct ataaaattgc tcagcgtttc ggtaactttg aagcaatcgg
    25021 accaattcta caaggcttga acaaaccggt ttctgatttg tctcgtggag caaatgaaga
    25081 agatgtatat aaattatcga ttatcacagc agcacaaaca ttgattgatt aacaaataca
    25141 cgcgaactag ccaaaaacag cagctgctgt tcttgactag ttcatttttt ctttaaaaaa
    25201 acttcattac gcgatatact ggcaaaaaga aagaagagcg ggagaagatg aaaaatgatc
    25261 gaactatcag gattaacaat gacagaacaa ttcgcagagg caattggtca ttccgcaatg
    25321 ccaggtgaca atctgatatt gactggtgat ctaggtgcgg gtaaaacaac gttgacgaaa
    25381 ggaattgccc aaggattagg gataacgcaa atgatcaaaa gtccgacata cacgattatt
    25441 agagaatata gtcaaggtcg gattccgttg tatcatatgg atatttatcg ggtagcagct
    25501 agcggagcag atttagggtt agatgaatat tttgaaggtg atgggctttc cgttgttgaa
    25561 tggggaaatc tattagaaga agcactgccg gaggattatt tggaactgat tttagaaaaa
    25621 agtgatacag atttagaaaa gcggtatgtg aaactaatct catacgggga gcaaagtgaa
    25681 accttcaaac agcgaattct agaaaaatgg gctgaaaatc atgagtgaag aagttgggat
    25741 catcttgcgg gaagcagtgc caggagatgc aaaagacatt cttttgatga tgggacaagt
    25801 taataaggag acagaatttt tagttttgga tgaagcagaa ctcctgttac caccagaaac
    25861 cttagaagaa gagttggatt atatatacga aagcaataat aatttattgc tgcttgcaat
    25921 ctatgaaggg acgattattg gaacagcatc tgtcaaggca gacagtcaat ttcgtttgtc
    25981 tcacgtggga gaagtcggta tcagtattct gcaggaatat tggggaatgg gactaggcac
    26041 tcttatgcta gaagaaatca tcagttgggc gaaggaaatg ggtgtacttt ttcgattgga
    26101 gcttgacgtt caagttcgta atgaaagagc cgtacacctt taccgaaaaa tgggcttcca
    26161 aatcgaagcg gtcatgccta gaggggcaag aacagatcta ggtgaatttt tagatgtata
    26221 taaaatgagt tatttgattg aatagaagaa ataggaaaag agtgagaaac tgtcatcctt
    26281 ttgagaaggc agaaaaacac acgaagggac tgtgacaaaa gtcgtgtcgc agtccatttt
    26341 ttttgaataa acggtgttca atcagctgac cttcggtatt agctcaaaaa acggcaaaat
    26401 atgaggaact attttgccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa
    26461 cctcttggta aacggtgtcc aaaagcttgc atctgcggta ataagtccaa ccaaatgaaa
    26521 aatatgagaa gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
    26581 caacctctcc aataaacggt gttctcctgc cagctcctcg gtcaccagtc acaatattta
    26641 ataaacatga gaagctattt tttgaaattg ctccttgtga ctcggagtga aacggcaggc
    26701 tcacgacctc tttgtgtgtt tcataagtat tcttttactt tatagtcaca agttttttca
    26761 atggatctgt cccgaatttt ttttcttgat aaagcaggat ttccgcacat gcacgactat
    26821 cttccaatgc atcatggtga tgatctaatt ggatgttcaa attttcgcac acagtgttca
    26881 atcgatgatt tggaaattca ggaaacaggc gacggctact tttcactgta cataaagaaa
    26941 gataattagg ctgttcaaga tgataataat ctaagcatcc cgctaagacc tttgtatcaa
    27001 aagcagcatt atgtgcaacg actaaacgat ttttttgaaa atatggttgg atagtttgcc
    27061 agacttctgg gaatttcggc gcattgcgta cgtcttcttg atggattccg tgaatttgga
    27121 catttcgcca gaaaaaagga gtctcaggct gtatcaatgt atagaattca tcaacgatct
    27181 ggctattttt taccatgact agcgcaagag aacaagcact ataaggctga tgattggcag
    27241 tttcaaaatc catggcaaca aaattcattt ctgtaaaacc tccttgggaa attactgtaa
    27301 catacttttg aaaaaagcaa ataaatttaa caaaaaacat agaatttttt atgtaatatt
    27361 tgttgagaaa agctagtttt tgaactcatt ccttgtgatt tggagttaga tggggatcat
    27421 gacttctcgg tattaagtca aatttatgca aagttgtgag gaaccatttt caaaaaaatt
    27481 tcttacgact cgaaagtagg cgactttttc acaacttctc cactaaacgg tgttcaatca
    27541 gctgaccctc ggtattagct caaaaaatag caaaatatga ggaactattt tgccatttgt
    27601 ttctctaata ctcaggtcag gacgctgatt tcacaacttc tcaataaacg gtgttcaaaa
    27661 gcttgcatct gcggtaataa gtccaaccaa acgaaaaata tgaggaacta tttttgtttg
    27721 gctgttctta ttacttgatg cagtgcagct ttttcacaac ctcttaataa acggtgttca
    27781 aaagcttgta tctgcggtaa taagcccaac caaacgaaaa atatgaggaa ctatttttgt
    27841 ttggctgttc ttattacttg atgcaggaca gctttttcac aatctctcga taaacagtgt
    27901 tcaaaagcca gtttctcggt attatgccaa atttatgcaa aatgtggaaa gccattttca
    27961 caaaattttt cttaatactc gaaactaaac gactttttca caacctcttt atttcaaatc
    28021 taattctact ggacaatgat cactgcccat aatgtccgta tggatctttg catcagctag
    28081 tcctggctct aaatctttgc tcgtcaaaaa gtaatcgatc cgccaaccag cattgttttt
    28141 ccttgaattg aatcggtagt tccaccaaga gtagacacct tcctgggttg gataaaaata
    28201 acggaatgta tcgataaacc cgttatccaa cagtcgagaa agagccgctc tttcttctgg
    28261 agtgaatcca gcattttttt gattcgtttt ccagtttttc aaatcaatct tttgatgagc
    28321 gacatttaga tccccacaca cgatgactgg tttttgtttt tttagatttt ccaaataatt
    28381 ataaaaggct tcttcccatt ctaatcggta atctaaacgt tttaattcgc tttgagaatt
    28441 tggcgtgtaa caggttacaa gaaagaaatc ggaatattcc aaagtaatca gacggccttc
    28501 tgtatcatgg atatcgatac ccattccgta agtggcattc aatgctggtt ttttagcaaa
    28561 aatcgctgtg ccggaatagc cttttcgttc cgcgtaattc caatattgat aataacctgg
    28621 aagatctaaa tcaatttgtc ctgcttggag tttcgtttcc tgcagacaga aaaaatcggc
    28681 gtctaactca tgaaaaactt ctaaaaaatt tttatttacg atggcacgaa gcccgtttac
    28741 gttccaagag ataaatttca ataaaagcac actccttcga attaatttcc ttacctttgc
    28801 gatactattg tacctcattt tagtaaaaaa tggtgtaaga tgaaagtaac taaatgatcc
    28861 aggaggaaag tagatgaaga ttggtgtaat cggtgtaggg aatatcgcag aaaaagcata
    28921 tttaccaact tatgcgaaaa aacaaggaac acttgatttt tattttgcaa caagaaataa
    28981 agaaacaaaa gaaagaattc aaaaagagta cgggttccaa catctatatg aaacgatcga
    29041 tgagctttta gaagaaaaaa tcgaagcatg tatgatccac gcagcaacaa aagtacatta
    29101 tgaattagcc aaaaaatgtc tagaaaatgg tatccatgtt tatattgaca aaccactgag
    29161 tgtccatcta aatgaagttc gtgaattgca agaattagct gataagaacc aaataatttt
    29221 gatgatcggt ttcaaccggc gctttgctcc tatggtagaa gaattgaaac gtatcccaga
    29281 taagcggttg atccacctgc aaaaaaatcg aatagcggca aaagaaacga cagagttcgt
    29341 cctttatgat ttgtttttac atcttattga tacagctgtg tatttattag atgaacccat
    29401 tttgagaaca aaataccaga taagagaaac aaaggaattt ttggaatatg ccattttaca
    29461 attagaaaca caaaatcaaa cggctatttt aacgatggat ttagcaagtg gcgctaatat
    29521 agaaaaatat caagtaacaa gtaaaaatgg tacttatgaa gtaaacgatc tgacccagct
    29581 tgtcattcaa gatgctgatg gaaaaaaagt gaaagaattt ggcgactgga caaacacgct
    29641 agtcaaacgc gggtttgagc caatggtaga ggccttcttt tctcagatcc gtttaggtaa
    29701 atcggaaaca gaattgctta aacaaaaaga agttgtaaga agtcatgaat tatgtgagga
    29761 agtgcttaaa gatcactacc gtcatcaact ttgaacaaga ctggcaagat tgtattaagc
    29821 gtgatagaat aatgaggacg tttgagaaaa gaaaggaaaa tcctcagtga ataaagaaga
    29881 aatagttgaa agcttagcca cgattactat attgtttgat gaaccattga tgaactatac
    29941 gtttacgaaa acaggtggac cagctgatgt ccttgcattc ccgaaaaaac aagaagaagt
    30001 aaagcaaatc atcgattatt gccgaatcca tgatatacct tggatggtat taggaaatgc
    30061 aagcaatctg atcgttcaag atggcgggat tcgcggtgta gtcatcatgc tgactgaaat
    30121 gaaacagatt catgtcaaag gaacgatggt cattgcggaa gcaggtgcct cactgattga
    30181 tacgacttat gcagctttgg cagaatcatt gactggattt gaatttgctt gcggcatccc
    30241 gggaagtgtc ggtggagcgg tttatatgaa tgcaggtgcg tatggcggag agatcaaaga
    30301 tgtttttgct gaagtggatc ttttgcttga agatggaaca ttgaaaactt tgacaaaaga
    30361 cgaaatggta ttttcttatc gtcacagtaa agtccaagaa ttacgtgcta tcgtattgga
    30421 agctcgattc agtttgcaaa caggggatta tgaagcaatc aaagcaagaa tggatgagtt
    30481 gaccgaattg cgtcaatcaa aacagccatt agaataccct tcatgcggaa gtgtctttaa
    30541 gcgcccagta ggacactata caggtcagct gatccaacaa gcaggattgc aaggcctgaa
    30601 atggggcggt gcacaagtct ctgaaaaaca tgctggattc atcgtcaata tcgatcatgc
    30661 gacagcgaca gattatattg aattgattgc gcacatccaa gaagtaatca aagaaaaatt
    30721 tgatgtgtct ttagaaacag aagtgcggat cattggagaa aaacaagcaa tatcagaaac
    30781 aagcagaagt ttagccaatt aataagtaat ggaacttaaa ataagtaaaa gagcaggacg
    30841 gtggccgtcc tgctcttttt tgtggttgtt tacgcaaat
//
LOCUS       DAFFSV010000019        30811 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFFSV010000019 DAFFSV010000000
VERSION     DAFFSV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.019"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     regulatory      <1..69
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            185..766
                     /locus_tag="LR110_000788"
     CDS             185..766
                     /locus_tag="LR110_000788"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBL4821789.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            769..2076
                     /locus_tag="LR110_000789"
     CDS             769..2076
                     /locus_tag="LR110_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBL4821790.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2492..2980
                     /gene="purE"
                     /locus_tag="LR110_000790"
     CDS             2492..2980
                     /gene="purE"
                     /locus_tag="LR110_000790"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBL4821791.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2973..4097
                     /gene="purK"
                     /locus_tag="LR110_000791"
     CDS             2973..4097
                     /gene="purK"
                     /locus_tag="LR110_000791"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBL4821792.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4110..5405
                     /gene="purB"
                     /locus_tag="LR110_000792"
     CDS             4110..5405
                     /gene="purB"
                     /locus_tag="LR110_000792"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBL4821793.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5585..5962)
                     /locus_tag="LR110_000793"
     CDS             complement(5585..5962)
                     /locus_tag="LR110_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821794.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            6218..6946
                     /locus_tag="LR110_000794"
     CDS             6218..6946
                     /locus_tag="LR110_000794"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBL4821795.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            6946..7200
                     /gene="purS"
                     /locus_tag="LR110_000795"
     CDS             6946..7200
                     /gene="purS"
                     /locus_tag="LR110_000795"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBL4821796.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            7202..7873
                     /gene="purQ"
                     /locus_tag="LR110_000796"
     CDS             7202..7873
                     /gene="purQ"
                     /locus_tag="LR110_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBL4821797.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            7874..10096
                     /gene="purL"
                     /locus_tag="LR110_000797"
     CDS             7874..10096
                     /gene="purL"
                     /locus_tag="LR110_000797"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBL4821798.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            10081..11520
                     /locus_tag="LR110_000798"
     CDS             10081..11520
                     /locus_tag="LR110_000798"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBL4821799.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            11543..12595
                     /locus_tag="LR110_000799"
     CDS             11543..12595
                     /locus_tag="LR110_000799"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBL4821800.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            12592..13170
                     /gene="purN"
                     /locus_tag="LR110_000800"
     CDS             12592..13170
                     /gene="purN"
                     /locus_tag="LR110_000800"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBL4821801.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            13167..14702
                     /gene="purH"
                     /locus_tag="LR110_000801"
     CDS             13167..14702
                     /gene="purH"
                     /locus_tag="LR110_000801"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBL4821802.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            14718..15992
                     /gene="purD"
                     /locus_tag="LR110_000802"
     CDS             14718..15992
                     /gene="purD"
                     /locus_tag="LR110_000802"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBL4821803.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            16114..17028
                     /locus_tag="LR110_000803"
     CDS             16114..17028
                     /locus_tag="LR110_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL4821804.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(17161..18726)
                     /locus_tag="LR110_000804"
     CDS             complement(17161..18726)
                     /locus_tag="LR110_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL4821805.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(18719..20170)
                     /locus_tag="LR110_000805"
     CDS             complement(18719..20170)
                     /locus_tag="LR110_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL4821806.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(20184..21200)
                     /locus_tag="LR110_000806"
     CDS             complement(20184..21200)
                     /locus_tag="LR110_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4821807.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            21420..22508
                     /locus_tag="LR110_000807"
     CDS             21420..22508
                     /locus_tag="LR110_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBL4821808.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            22562..24088
                     /locus_tag="LR110_000808"
     CDS             22562..24088
                     /locus_tag="LR110_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBL4821809.1"
                     /translation="MADCFLKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            24090..25295
                     /locus_tag="LR110_000809"
     CDS             24090..25295
                     /locus_tag="LR110_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4821810.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            25552..26529
                     /gene="guaC"
                     /locus_tag="LR110_000810"
     CDS             25552..26529
                     /gene="guaC"
                     /locus_tag="LR110_000810"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBL4821811.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            26793..28217
                     /locus_tag="LR110_000811"
     CDS             26793..28217
                     /locus_tag="LR110_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL4821812.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            28678..29787
                     /gene="pdhA"
                     /locus_tag="LR110_000812"
     CDS             28678..29787
                     /gene="pdhA"
                     /locus_tag="LR110_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBL4821813.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            29790..30767
                     /locus_tag="LR110_000813"
     CDS             29790..30767
                     /locus_tag="LR110_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBL4821814.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         9892 a         5249 c         6978 g         8692 t
ORIGIN      
        1 gtcataatat ggatgacagt ttctagccag taccgtaaat gctggactat aagtaaaaga
       61 ttggctattt cacccttctt ttcttatgat aaagtggagg gtgtttttct ttaacagaaa
      121 catagtaggt ttttgcacaa aagcggttat ttcggtatta ataaatgata aaggagcaaa
      181 taaagtgaaa gaattagttg aacgtattca aaaagacgga cgtatattag gcgagggcgt
      241 attaaaagtc gacagcttcg tcactcacca agtagaccct gtattgatgg aacaaatggg
      301 aaaacggttt gcagaagtat ttaaagaaca aggaatcacg aaagttgtga ccattgaagc
      361 ttctgggatc gcaccagctt tatttgctgc aagagaatta gatgttccaa tgatcttcgc
      421 tagaaaagca aaaagtttga cgatggatga agaattattg acagcttctg tttattcttt
      481 tacgaaacaa gtgacaagta cgatttctat ttctcgtaaa tttttatctt cagaagacaa
      541 agtattgatt atcgacgatt tcttggcaaa cggacaagcg gcaaaaggat tgatcgaatt
      601 atgccagcaa gctggagcgc aagtagaagg aatcgggatc gtcatcgaaa agtctttcca
      661 agatggacgt cagttacttg aagaaatggg attgagagtc gtttctcttg cgcgtattgc
      721 ttccttgacg aatggacaag ttgagtttct tgaggaggac gcataatcgt ggaaaaagaa
      781 acacaaaatg gaaaagctgc ggttttaggg ctgcagcatc tgctagcgat gtatgcagga
      841 gcagtagcgg tgcctctttt gatcggtaca ggactaggct ttaatcaaga acagatgact
      901 tatttgattt caattgatat ctttatgtgt ggattagcga cattattgca attgacagtt
      961 aaccgatttt tcggaattgg cttgcctgtc gttttaggat gtgcgatcca agcagtagct
     1021 ccactgatct tgatcggaac caatgaagga gtaggagcta tctacggatc tattatcgct
     1081 tctgggatct ttgttgtgtt agtttctggt gtcttttcta aaattaaaaa actattccct
     1141 cctatagtaa caggaacggt tatcaccgtt attggtttga ctttgattcc tgtagcaatc
     1201 gagaaaatgg gtggaggtaa tgcaacagca gctgattttg gtgacaaaac aaatctgcta
     1261 cttgcatttg tgacgattct cctaattgta ggcgtgcaaa tgctggcaaa agggttcatt
     1321 cgttcaatcg cggtattgat tggtttagtc ggaggaagca tcttagctgc tttcatggga
     1381 atgattgatc tgagcgcgat tggagatgcg ccgttgttcc atgttccaca acccttttat
     1441 tttggtaaac caactttcga cgtatggtcg attttactaa tgatcatcat ttccatggtc
     1501 agcatggtag aatcaacagg tgtatatttt gcacttggtg acattactgg gaaaaatgtt
     1561 ggagaagaag aattgaaaaa gggatatcgt gcagaaggac ttgccgttat cttaggcggt
     1621 attttcaata cattccctta tactggcttt tctcaaaatg tcggattggt tcaactttcg
     1681 ggaatcaaaa caagaaaacc catctatttc tctgcattct tcttgattgt tttaggatta
     1741 ttccctaaaa tcggtgcgtt agcacaaatc atccctgagc cggtacttgg tggaggaatg
     1801 ttagtcatgt tcgggatggt ggcagtccaa ggaatccgta tgctgttgga agttgatttc
     1861 agcaatgata aaaatctatt gattgttgct gtttctgttg gatttgggct gggtttcaat
     1921 gttacgccga cattattcag tcaaatgccg caaacattgc aaatgttcac tggaaatggc
     1981 atcgtaatga gtagcattac ggctatcata ttgaatttga tctttaatgg attgaaaaaa
     2041 gatgaagaaa tattggaaga agccttcaag aagtaataga gcaaaagaaa ggttgtgaaa
     2101 tcagcgtttt gacctgattg ttagagaaac aaacggaaaa atagttcctc gtattttgcc
     2161 gtttgtttga gctaataccg agggtcagct gattgaacac cgttgaagga agaggtcgtg
     2221 agcctgccgt tcatctccga gtcacaagga acaatttcaa aaaaaagctt ctcatgtttg
     2281 ttgaatattg ttccttgtga ccgaggagtt ggcaggagaa caccgtttat ccataaaaag
     2341 gaatgtgacc taacttttgt cacattcctt ttttttatct ttaataaaca cgaacaataa
     2401 aatgtttttc tttgttaaca ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa
     2461 tatgaatgta gaaaaactgg gaggaaaaac aatgacagca aaaatctctg tgattatggg
     2521 gagtacttca gattgggaaa caatgaaaga agcatgtcaa atattggatg aattcggtgt
     2581 gtcttatgaa aaaaaggtcg tttctgctca tcgaacccca gatttgatgt ttgaatatgc
     2641 acaaaacgct cgaaaaaaag gaatcaaaat cattattgca ggagcagggg gagccgcgca
     2701 tctgccgggt atggtcgcat caaaaacaac tttgccggtt atcggtgtac cggtccaatc
     2761 acgaacatta aatggtttgg attcgcttct ttcgattgtc caaatgcctg gaggagtacc
     2821 tgtagctact acagcaatcg gaaaagcggg agcaattaat gctggactac ttgcgataca
     2881 gatgctttcc atgtatgatt tagaattgga ggctaaattg gctgaaagac gttcaatgtt
     2941 ggctaaaact gttatagaaa gtagtgatca acttggttaa acctttgttg cctggttcaa
     3001 cgatcgggat cgtcggtggc ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa
     3061 tgggctttaa agtaggtgtg ctggatcctg tgagcgattg tccagccgca caagttgcag
     3121 attggcacat tgaaagtgtc tatgatgaca cctttgcgct gaaagagctt gccagaagaa
     3181 cagatgtcat tacctatgaa ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac
     3241 ccatgtcatt tattcctcaa gggacagact tgcttgcgat tacgcaagac cgcctgttag
     3301 aaaaatcttt tttggaaacg aataacatcg tgattgctcc ttatgcaacg atcgtcagtc
     3361 cgacggatat ccaagatgcg attgacggga tcggctatcc ttgtgttttg aaaaccactc
     3421 gaggcggcta tgatggcaag gggcagtatg ttttgaaaag tcgggcggat ctagctcctg
     3481 ccatggacct tttaagagaa ggaacctgtg ttttagaagc atggatcccg tttgaaaaag
     3541 agatttcgat catggtggca ggaaatggtc aaggagattt cacgactttt ccggtcgtgg
     3601 aaaacatcca ccacaacaat attcttcatg aaacgattgc tccagccgcg attgatcagg
     3661 atgtgatcga ggaggctgag cgtattgccc gtgtgattgc agaagcagtg agcctttcag
     3721 gtgttttagg ggtagaaatg tttttaacaa aaacaggcgg gctatatgtg aatgaattag
     3781 ctcctcgtcc tcacaattct ggacattatt ctattgaagc ttgttctatg agtcagttcg
     3841 atgcccatat tcgaggtatt tgtggttggc cattaggaga aaaagtacgt ttgataagca
     3901 atgcagtaat ggtcaatgtt ttgggagaac aactgctgga aagctatcgc ttgattgaga
     3961 ataagccaga ctggcaattc catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga
     4021 tgggacatat cactatcttg acagaagaca tccagcaaac gctggaagaa atcaaacaaa
     4081 cgaatatttg ggattagagg agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg
     4141 ggaatatctg gacggaagag aacaaatacc gtgcgtggct cgaagtcgag attttagcag
     4201 atgaagcttg ggcagaactt ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc
     4261 atgcatcatt tgatatcgat cggatcttgg aaatcgaaaa attaacacgc catgatgtcg
     4321 ttgcgtttac acgagcggtt tctgaaacat tgggagaaga aagaaaatgg gtccattatg
     4381 gtctaacaag tacggatgtc gtagatactg cttatggcta tctcatcaag caagcaaatg
     4441 atattttacg ggaagacttg aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac
     4501 acaaatatac agtgatgatg ggtcggacac acggtgtcca tgctgaacca actacttttg
     4561 gattgaaact tgccacatgg tattcagaaa tgaaacggaa catcgaacga tttgaacatg
     4621 cggcaaaagg agtagaagca ggaaaaatct caggagcagt gggaacgttc gcaaatatcc
     4681 cgccatttat tgaagagtat gtatgtaaac aattggggat ccgtccacaa gaaatttcta
     4741 ctcaagttct ccctcgggat ctacatgctg aatatttctc agcgatggcg cttattgcta
     4801 caagtattga acgttttgca acagagatac gcggattgca aaaatccgaa acacgtgaag
     4861 tagaagaatt ttttgcgaaa ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc
     4921 cgatcggttc agaaaatatg gctggtctag ccagagtggt gcgcggacac atggtcactg
     4981 cctatgaaaa tgtcacatta tggcatgaaa gagatatttc tcattcttca gcagaacgaa
     5041 tcatcttgcc tgataccacg attttattga attacatgtt aaatcgtttt ggcaatatcg
     5101 ttaaaaatct aacggtcttt cctgaaaaca tgaagcggaa tatgtctgca acatttggtt
     5161 tgatttatag ccagcgcgtt atgctgaaat tgatcgataa gggaatgaca agagaagaag
     5221 cttatgatct tgttcagcca aaaactgcgt acgcttggga caaccaagtg cagttccgcc
     5281 cgctgttaga agaagatcca accatcacat cggtattatc taaagaagaa atagacgatg
     5341 cctttgatta tcatcatcat ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg
     5401 aataaagaag agtgagttgc aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa
     5461 gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt atcaataata
     5521 ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac aaaaaataga
     5581 ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg agatgttgca
     5641 gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc tggatacgat
     5701 aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt
     5761 ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt gctcggcgga
     5821 tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct ttgatcgaca
     5881 gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa aaaagctgac
     5941 cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc caaattttta
     6001 gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg gtataagatt
     6061 ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt acatattttt
     6121 gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg
     6181 tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag aacttttgta
     6241 tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt ggatagaata
     6301 tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg gaaaaggaga
     6361 attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag gaataaaaaa
     6421 tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac aaattatccc
     6481 tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc taggtgttcc
     6541 cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg accagttgga
     6601 tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc cggaagaaat
     6661 cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat tatttgctaa
     6721 ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg gacaaatttt
     6781 gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta cgaatgaaca
     6841 tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct atcaagaagt
     6901 atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta tttcgtaaaa
     6961 gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca
     7021 gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata ctttgagata
     7081 caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg tgacaagctg
     7141 cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga ggaagtataa
     7201 catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata tgttatgggc
     7261 agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt cctctttaga
     7321 aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt tgagatgcgg
     7381 tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg aagaagggaa
     7441 accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac ttttgccagg
     7501 tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa
     7561 ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc ttccagttgc
     7621 tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa aaaaaaataa
     7681 tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca ttgcagggat
     7741 tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg cagtagaaga
     7801 cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa
     7861 ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca agaaaaaaag
     7921 aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag aagatatctt
     7981 aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga gcgaacattg
     8041 ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac ctcatgtgtt
     8101 gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag cagttgtctt
     8161 caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag ctgcgacagg
     8221 agttggcgga atcatcagag atattttcag catgggggct cgtccaattg cgttgctaga
     8281 ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag aagaagtagt
     8341 tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg gcgaaattgc
     8401 atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag gtttgattga
     8461 acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg
     8521 tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag aatttaatca
     8581 agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg aaaagctgct
     8641 gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag gtatacagga
     8701 tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag ctggctcagg
     8761 acttatattg acattagatg aagtcccaca aagagaaact gggatgacac cttatgaaat
     8821 gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa
     8881 aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta aagtaacaga
     8941 cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc cagtcgatgc
     9001 attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc gaatcgtaga
     9061 atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag tattgcttga
     9121 tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt atgattcaca
     9181 agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga gggttagagg
     9241 gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt atttggatcc
     9301 aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg ctagcggtgg
     9361 taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac cagaagtttt
     9421 ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc
     9481 tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa tctatccaac
     9541 accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta ctcaggaatt
     9601 caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt ttgatggcag
     9661 tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact ttgatctatc
     9721 cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat tgattgaaag
     9781 tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag ctttcaaaca
     9841 tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg cagaaactca
     9901 atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag aaatgatggg
     9961 agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac
    10021 tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg atgcaatcgt
    10081 atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat
    10141 ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg aggacaagaa
    10201 ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga tttggggctg
    10261 ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc
    10321 ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca gccttttcta
    10381 ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat caatgctcgt
    10441 tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc cgatacggag
    10501 atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa attaaaaagt
    10561 gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac ggcaatgatt
    10621 gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca
    10681 tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt tataagagat
    10741 gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga ttgctttacg
    10801 aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag accagattca
    10861 aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa tttagcgaaa
    10921 gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt gtcagccgca
    10981 agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa aaatcaatac
    11041 attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa
    11101 ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga tgattcaatt
    11161 gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg ggcaaaagaa
    11221 gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg gattgatatt
    11281 caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa agaatgtatc
    11341 ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat cggtctggaa
    11401 aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta tccgacgccg
    11461 ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc gttcttttaa
    11521 aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa aagctggcgt
    11581 agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca aaaaaacgga
    11641 acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt tatcggtctt
    11701 tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga aattagcagt
    11761 ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa
    11821 tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag caacggggaa
    11881 aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg
    11941 aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact caggtaatga
    12001 ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat tgacaggaga
    12061 aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc attcaaatgg
    12121 gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat catcaaaact
    12181 gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc
    12241 ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata tcactggtgg
    12301 agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac aaatcaaact
    12361 tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg aaattcctga
    12421 gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag tcgaagaatc
    12481 gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt atgaaatcgg
    12541 gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac gatgagaatc
    12601 gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta tctatcgaaa
    12661 aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc atatgttttg
    12721 aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga atttgattca
    12781 aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat cgatttgatc
    12841 gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta tgataaaaga
    12901 atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg catacgtgat
    12961 gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga ccaaggtgta
    13021 gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga cacgttcgat
    13081 tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt gattagtgaa
    13141 attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta tcagataaaa
    13201 ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc atttcaacag
    13261 gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc gaagaagtga
    13321 cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa atccatggtg
    13381 gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg gaaaatatcc
    13441 aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt atgaaaccag
    13501 atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt atgctccgta
    13561 gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac tatgaagaag
    13621 tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa aaactagcgg
    13681 caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac ttaacggatt
    13741 gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa caaacgcttc
    13801 gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt ctggaaaaat
    13861 attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat aatatccgag
    13921 atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta gtcgccttga
    13981 aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg tgggaagcag
    14041 cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat cgagaagtag
    14101 atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt gccccttctt
    14161 atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta ctgacagtag
    14221 actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta ggtggacttc
    14281 ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac
    14341 gtaagccgac tccagcagaa gcaaaagcat tagtgtttgc ttggagagct gtcaaacatg
    14401 tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc ggagccgggc
    14461 agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag aaaatggaag
    14521 gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa tatgcagcca
    14581 aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa gattctattg
    14641 atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat tttagacatt
    14701 agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa
    14761 taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg ccaggaaatc
    14821 cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat cattcagcgt
    14881 taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct gaagttcctt
    14941 tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc ggtccgacaa
    15001 aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg gatacttatc
    15061 aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca gcgtatatca
    15121 aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg aaaggtgtag
    15181 tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg aaccagcatc
    15241 gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga gaagaattct
    15301 cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg caagatcata
    15361 agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct tattcaccag
    15421 taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta aaaccagcgg
    15481 ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg ggtttgattg
    15541 ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc
    15601 aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat ttattagaga
    15661 atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag
    15721 ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt ctggaagaag
    15781 agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt ggagcaggag
    15841 gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag aaaaaaattt
    15901 acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat attggagaaa
    15961 aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta taaaaaatac
    16021 aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag tgtggtacac
    16081 ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt aggtacttct
    16141 gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca
    16201 gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca tgcagatatt
    16261 tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa
    16321 cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt
    16381 tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa aatggattat
    16441 gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga agtagctgaa
    16501 gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa
    16561 ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc taatcagctg
    16621 caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa tatgacagat
    16681 gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca tgatatgacg
    16741 atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat cgggaacgaa
    16801 aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca aacgacacca
    16861 actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt gatcgccggt
    16921 acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa tatgtctcgg
    16981 gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag attgtcataa
    17041 gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt tttcagcttc
    17101 gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg atttcgttta
    17161 ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag aaatcccttg
    17221 ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt caaaagcaat
    17281 atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga ttctttgttc
    17341 tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga
    17401 gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat cggtaaaatt
    17461 ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc ttgaaggctg
    17521 tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt aaagcgagtc
    17581 gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt cttgcgggat
    17641 tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata aacgatataa
    17701 tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact tagatacttc
    17761 gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag aatcaactgg
    17821 aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa gggaactggc
    17881 ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga ttctttctgc
    17941 gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt catggaccaa
    18001 cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat caactattaa
    18061 aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct ccctaaataa
    18121 aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga cttggtttgc
    18181 tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt cagcaagttt
    18241 caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat gatttttttt
    18301 caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt
    18361 tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa gcagataatc
    18421 atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa
    18481 tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca acccgtccat
    18541 gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa ttgaagacag
    18601 tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag aaccaaggac
    18661 agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga taaaaacttt
    18721 atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt gtgacagttg
    18781 ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg taataaagaa
    18841 ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa tcgatccctt
    18901 tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc ccgttgtcta
    18961 taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata aatcctttgt
    19021 gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc aagatcattt
    19081 ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca agcatatggc
    19141 cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct ttttcaagag
    19201 gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca
    19261 tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca tccaaactgt
    19321 tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt tgtgctaaat
    19381 ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc attttattca
    19441 tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga aagccaaggt
    19501 tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct tggacctttc
    19561 tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga actccaagtt
    19621 ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg cttgctaagt
    19681 cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg acagaatcta
    19741 cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata ttattgatca
    19801 gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct aacgtactaa
    19861 tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta agttcttcta
    19921 ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag tcccacatac
    19981 tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg atatttgcga
    20041 tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt agcaaaggga
    20101 taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt atgcttaaat
    20161 tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc tatattctgc
    20221 tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc tgtttgaata
    20281 tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg tttacactcg
    20341 actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg tccatcaata
    20401 gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc agctaaagca
    20461 ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa atcatttgtt
    20521 tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc gacaattcgg
    20581 tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag ggttggttta
    20641 cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga gttctctgaa
    20701 ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg attgctgaca
    20761 aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt gacgaaaagc
    20821 tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc tgctcgcttt
    20881 gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat gatcatatcc
    20941 actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt catgtagcca
    21001 tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga ttcagtatgc
    21061 tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag tgggtcaggt
    21121 gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac aagggctaaa
    21181 aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta ctatcttatc
    21241 agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa
    21301 aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat aaaaaaatca
    21361 atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa
    21421 tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca agcattctat
    21481 taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta ggaatcgcta
    21541 tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc tcggaattag
    21601 aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa aatcaagtag
    21661 cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct tctatcgatg
    21721 gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag gtgatcgcct
    21781 atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt gataattttg
    21841 gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa gaaggaaaag
    21901 gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca ctaatcaatt
    21961 acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg gtcgtgccat
    22021 ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct acggcgcaag
    22081 caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat gctgttttgt
    22141 cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga
    22201 gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct ggcgttaaat
    22261 cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc ttagcaaaaa
    22321 atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc aatgatactg
    22381 aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca gtatctgtcg
    22441 ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa tccgatctga
    22501 aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga ggataagaat
    22561 tatggcggat tgttttttaa aaatggcaga tatcaccaaa aaattttctg gaatccgagc
    22621 gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt gcggagaaaa
    22681 cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat atggctccta
    22741 tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga aagatagtga
    22801 agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc tttcaataaa
    22861 agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt gggaactgac
    22921 agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc cgaatcttct
    22981 agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag cattatcaaa
    23041 atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag aagaaagcgc
    23101 gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca tcattatttc
    23161 acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac gggatggaca
    23221 gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc gcggaatggt
    23281 gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag aagtttattt
    23341 taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca taaacgacca
    23401 catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga tgggagcggg
    23461 cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga tatccggacg
    23521 tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca ttgcaaacgg
    23581 actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga tggatattcg
    23641 ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat tagataaaga
    23701 aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa caaactccat
    23761 ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg
    23821 gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga tagatgttgg
    23881 ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca aagctgtctg
    23941 tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct atacgatgaa
    24001 cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag tcttgatgaa
    24061 attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt acggataaaa
    24121 aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc tacagcatgg
    24181 tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt attttctggc
    24241 gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta ctagctgcag
    24301 ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt
    24361 ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg tggatgacag
    24421 ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt
    24481 atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt cgtggactga
    24541 cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc caaaaaatgt
    24601 ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg acattgattt
    24661 taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga
    24721 aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg gtatttacag
    24781 cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat ccgattattt
    24841 tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa acagtagcag
    24901 gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa
    24961 caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct ttagctggat
    25021 tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg atggaactgg
    25081 atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt ggaacaatca
    25141 tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg tcgattttag
    25201 gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta gccgttgtgc
    25261 tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa
    25321 gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat tcattgtttc
    25381 gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt ctctttttgc
    25441 tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa gccttgatta
    25501 aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt aatgaaagta
    25561 tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag ccgttctgaa
    25621 tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt gcctgccaac
    25681 atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg ttatttctat
    25741 atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat gaaaaagcgt
    25801 ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt tatagagaag
    25861 ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca tggacatgct
    25921 aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt
    25981 gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca
    26041 acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac aggctttggg
    26101 actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg caagccgatc
    26161 atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg ttttggtgcg
    26221 acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa
    26281 gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt ccaaaaaggc
    26341 gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc tttgaaagat
    26401 acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg aggaagagat
    26461 ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt taatggagac
    26521 acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct gcattaagta
    26581 ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga attattatcg
    26641 cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag atttttgtca
    26701 cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa
    26761 aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag aaaaaacaag
    26821 gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac ggaaggatca
    26881 atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt gtgaatcgtt
    26941 ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg agcaaacgtg
    27001 gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca agaatcgatt
    27061 ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa aaaataagag
    27121 aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag atgccagatg
    27181 agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta gcggaagaaa
    27241 aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg tatttggaaa
    27301 gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc ggtcagcaag
    27361 caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct gtagagtcac
    27421 cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta tttgggatac
    27481 cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat aaaaataaat
    27541 tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa
    27601 aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt attgaagacg
    27661 atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata cgaacgattg
    27721 atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa
    27781 tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg caagaaatga
    27841 tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt aatgaagcaa
    27901 ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg
    27961 cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt tactatgcct
    28021 ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta gtagatatgg
    28081 gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc agaatcaatt
    28141 acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa attctagaaa
    28201 agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca gcttggattt
    28261 aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa actaagaaaa
    28321 aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg caacaaaact
    28381 gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta gaaaatagaa
    28441 aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact aatgctgtta
    28501 atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag cttgtatttc
    28561 tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag ttttattcac
    28621 tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt gtgacaaatg
    28681 gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa tgcagatttc
    28741 ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt agtacctgat
    28801 ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg tgtattagac
    28861 caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc aactgctgga
    28921 caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt tcttcttcca
    28981 ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca agctttttta
    29041 tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc tctaccacca
    29101 caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg tttgaagaaa
    29161 cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc acaaggtgat
    29221 ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt ttatatccaa
    29281 aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca
    29341 caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga tccgcttgca
    29401 gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg accagtccta
    29461 atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga tccaacccgc
    29521 taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat ccgcttccgt
    29581 aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt catcgaagca
    29641 actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa acaaaaggtt
    29701 tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga acaaattgca
    29761 atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc caagcaatca
    29821 cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg
    29881 ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag
    29941 accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt ttcggtcttg
    30001 cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt ttcgaagcaa
    30061 tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc actagaaatc
    30121 tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa ttgcattcgg
    30181 ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt ccttccaacc
    30241 cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg gttgttttcc
    30301 tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa gcttacgaag
    30361 ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt atcacatacg
    30421 gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa aatatatctg
    30481 ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc attcaatccg
    30541 tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg
    30601 cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc ccaatcggac
    30661 gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc tggttgccaa
    30721 atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa aagaagggac
    30781 gaaacaaagt catggcttat caatttaaat t
//
LOCUS       DAFFSV010000020        30153 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFFSV010000020 DAFFSV010000000
VERSION     DAFFSV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.020"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..185)
                     /locus_tag="LR110_000814"
     CDS             complement(<1..185)
                     /locus_tag="LR110_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL4821815.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLY"
     gene            complement(221..565)
                     /locus_tag="LR110_000815"
     CDS             complement(221..565)
                     /locus_tag="LR110_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4821816.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            866..1087
                     /locus_tag="LR110_000816"
     CDS             866..1087
                     /locus_tag="LR110_000816"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821817.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            1214..1990
                     /locus_tag="LR110_000817"
     CDS             1214..1990
                     /locus_tag="LR110_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL4821818.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            1993..2181
                     /locus_tag="LR110_000818"
     CDS             1993..2181
                     /locus_tag="LR110_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4821819.1"
                     /translation="MSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRRKP
                     DKQIKPIEAALAELEKEK"
     gene            2183..2467
                     /locus_tag="LR110_000819"
     CDS             2183..2467
                     /locus_tag="LR110_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821820.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            2488..2676
                     /locus_tag="LR110_000820"
     CDS             2488..2676
                     /locus_tag="LR110_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821821.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2673..2846
                     /locus_tag="LR110_000821"
     CDS             2673..2846
                     /locus_tag="LR110_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821822.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            3002..3241
                     /locus_tag="LR110_000822"
     CDS             3002..3241
                     /locus_tag="LR110_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821823.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            3247..3933
                     /locus_tag="LR110_000823"
     CDS             3247..3933
                     /locus_tag="LR110_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821824.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            3936..4766
                     /locus_tag="LR110_000824"
     CDS             3936..4766
                     /locus_tag="LR110_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBL4821825.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            4783..5634
                     /locus_tag="LR110_000825"
     CDS             4783..5634
                     /locus_tag="LR110_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL4821826.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            5631..5990
                     /locus_tag="LR110_000826"
     CDS             5631..5990
                     /locus_tag="LR110_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBL4821827.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            6005..6166
                     /locus_tag="LR110_000827"
     CDS             6005..6166
                     /locus_tag="LR110_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL4821828.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            6163..6468
                     /locus_tag="LR110_000828"
     CDS             6163..6468
                     /locus_tag="LR110_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821829.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            6468..6824
                     /locus_tag="LR110_000829"
     CDS             6468..6824
                     /locus_tag="LR110_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBL4821830.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            6811..7029
                     /locus_tag="LR110_000830"
     CDS             6811..7029
                     /locus_tag="LR110_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821831.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            7050..7139
                     /locus_tag="LR110_000831"
                     /pseudo
     CDS             7050..7139
                     /locus_tag="LR110_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            7214..7681
                     /locus_tag="LR110_000832"
     CDS             7214..7681
                     /locus_tag="LR110_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBL4821832.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            7956..8192
                     /locus_tag="LR110_000833"
     CDS             7956..8192
                     /locus_tag="LR110_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821833.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            8216..8557
                     /locus_tag="LR110_000834"
     CDS             8216..8557
                     /locus_tag="LR110_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821834.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(8550..8741)
                     /locus_tag="LR110_000835"
     CDS             complement(8550..8741)
                     /locus_tag="LR110_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBL4821835.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            8951..9598
                     /locus_tag="LR110_000836"
     CDS             8951..9598
                     /locus_tag="LR110_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821836.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            9858..10202
                     /locus_tag="LR110_000837"
     CDS             9858..10202
                     /locus_tag="LR110_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL4821837.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            10207..10488
                     /locus_tag="LR110_000838"
     CDS             10207..10488
                     /locus_tag="LR110_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821838.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            10591..10905
                     /locus_tag="LR110_000839"
     CDS             10591..10905
                     /locus_tag="LR110_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBL4821839.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10883..12577
                     /locus_tag="LR110_000840"
     CDS             10883..12577
                     /locus_tag="LR110_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL4821840.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            12597..13775
                     /locus_tag="LR110_000841"
     CDS             12597..13775
                     /locus_tag="LR110_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL4821841.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            13738..14424
                     /locus_tag="LR110_000842"
     CDS             13738..14424
                     /locus_tag="LR110_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBL4821842.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            14424..15584
                     /locus_tag="LR110_000843"
     CDS             14424..15584
                     /locus_tag="LR110_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL4821843.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            15594..16469
                     /locus_tag="LR110_000844"
     CDS             15594..16469
                     /locus_tag="LR110_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821844.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            16466..16777
                     /locus_tag="LR110_000845"
     CDS             16466..16777
                     /locus_tag="LR110_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821845.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            16767..17120
                     /locus_tag="LR110_000846"
     CDS             16767..17120
                     /locus_tag="LR110_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL4821846.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            17110..17511
                     /locus_tag="LR110_000847"
     CDS             17110..17511
                     /locus_tag="LR110_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821847.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            17504..17908
                     /locus_tag="LR110_000848"
     CDS             17504..17908
                     /locus_tag="LR110_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821848.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            17920..18525
                     /locus_tag="LR110_000849"
     CDS             17920..18525
                     /locus_tag="LR110_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4821849.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            18545..18907
                     /locus_tag="LR110_000850"
     CDS             18545..18907
                     /locus_tag="LR110_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821850.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            18910..19092
                     /locus_tag="LR110_000851"
     CDS             18910..19092
                     /locus_tag="LR110_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821851.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            19109..22528
                     /locus_tag="LR110_000852"
     CDS             19109..22528
                     /locus_tag="LR110_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL4821852.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            22579..23316
                     /locus_tag="LR110_000853"
     CDS             22579..23316
                     /locus_tag="LR110_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4821853.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            23326..25617
                     /locus_tag="LR110_000854"
     CDS             23326..25617
                     /locus_tag="LR110_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4821854.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            25641..27731
                     /locus_tag="LR110_000855"
     CDS             25641..27731
                     /locus_tag="LR110_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821855.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            27748..27897
                     /locus_tag="LR110_000856"
     CDS             27748..27897
                     /locus_tag="LR110_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821856.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            27894..28340
                     /locus_tag="LR110_000857"
     CDS             27894..28340
                     /locus_tag="LR110_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821857.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            28342..28479
                     /locus_tag="LR110_000858"
     CDS             28342..28479
                     /locus_tag="LR110_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL4821858.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            28517..28810
                     /locus_tag="LR110_000859"
     CDS             28517..28810
                     /locus_tag="LR110_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821859.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            28807..29031
                     /locus_tag="LR110_000860"
     CDS             28807..29031
                     /locus_tag="LR110_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL4821860.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            29028..30053
                     /locus_tag="LR110_000861"
     CDS             29028..30053
                     /locus_tag="LR110_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL4821861.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT        10885 a         4555 c         6210 g         8503 t
ORIGIN      
        1 ttatataaaa aataattatc atgatgttta gaagcgtgtc ctaattcatg taataaggtt
       61 ctttgtcttc tatacttaga taaattagct tttattacta tggtattcat acaagcaaca
      121 taatggccat cggcatcaag gttttcacgc tcttctacct taacgccgag ttcattaatt
      181 atcatttcaa tttgactgtc caaatgactc acctacttat ttagttatct tttttctcta
      241 aatatgcttc aatcatagct gatagtattt ccctatcatt atcggtgagc ggttttccgt
      301 cgctactcat aacagatgct aaagcttctt ccacggttag ttttctttcc tctggtgcaa
      361 ctcccgcgtt aggatcatct gtgcgtccta gcaaataatc tgttgataca tggaagtagt
      421 cagcaacttt ttgcaaagtt tctattttag gactttgtgt cttccattta taaatagagt
      481 tttttccaaa tcctattcta ttttctaatt cagaaataga aatacctctt tttttacaga
      541 gctctttaac cctttcgaaa agcatgtcaa atcaaccttc ctagagcgta cgataataat
      601 ttatacaaaa agatagaaag gtagttgaca aatctatcca attgtcttat atttaactcg
      661 taagctaatt taataagcta acaagcaaca agaaatacac ctaataaaat aaacgaattc
      721 gaggtcggca aactaagaat gttaattttt agggtttttc taggcttatt taactgtata
      781 taaatataag acagttggat agaaatgtca ataaaaatta gctaatttta ttagcttaca
      841 tatttaaaga aaaggaggaa acatcatgaa acaaaataaa aaaactcctt ccgcaaaagg
      901 agctttaata actttaaatt ttaaagacca aaagtttagt gctgttgttg atgaaattca
      961 tccacaagag catcaaaaaa attcttttgc aactgacgag aaaaagactc agaaacgctc
     1021 tgagaaagtt cttcgaataa ttcaatttct gttggaaaag attcgtcatt ttggaagtct
     1081 attttgaatt ctttattaac agtcttgaat acggtttgtt tgaataaggc gtcgacatcc
     1141 atattaaatt cttccatact attttcacct cgctttctat tcaaaattat atcaaagaaa
     1201 ggaatgagca aaaatgaaca caccgcaaat ttttaacttt gaacaacatg aagtaagaac
     1261 agtaacaata catgatgaac cattttttgt tggaaaagat gtggctaaag ttctagggta
     1321 ccaaaatggt agtagagata ttaacagaca cgtcgatgta gaagatcggc aaaactacca
     1381 aaacggtact tttgaatcgc ctagagggtt aactattatt aacgaatcag gtttgtactc
     1441 cttaatttta ggaagcaaac aaccaaatgc taagaaattt aaacgttggg tgactagcga
     1501 agtgttgcca gcaattagaa aacatggagg ttatctaact ccagaaaaag tagaagaagc
     1561 tttgcttaat ccagatacaa tcatccaatt agcaactaag ttgaaagaag aacgtactgg
     1621 aagactaatc gcagaacaaa aaattgcaga atacgaaccc aaaatttcgt atttggatag
     1681 tatcttatct tctacagatt cagtaactat tagtcaaatc gcggctgatt atgggatgtc
     1741 tccacaacag atgaacaaac tacttcataa gctaggtatt cagaaaaaag taggcaacca
     1801 atggttatta tgcaaaaaac acatgagaca gggttacaca aaatctcata caactgagat
     1861 tccgaaatct gatggcggaa caaaagttgt gatgaataca aaatggactc aaaaagggcg
     1921 tctgttcatt tatgagtcgc tgaaaaaaga aggatatatt ccagaaatcg atttattaga
     1981 ggaggaatga cgatgtcaca agaatttatt ttgaaagtca gaattcaatt agcaaaatac
     2041 ggaaagtctc agaactggtt agctgatact atcggaattt ctagaccata tatgtcggac
     2101 attatgaatg ggcgtagaaa accagataaa caaatcaagc caattgaggc agcactagca
     2161 gagttagaga aggagaaata acatgcaaat aacaattcca gataatttag tagtttccga
     2221 attaactaca cagattacga atgctgttct taattcattg gacgaacgat tacaccttat
     2281 gaacaaatca gtagagcttc ctccatatcc aaacaaatca gaggtaaaaa aagttttagg
     2341 cattggtgat gacaaattaa cacattggat aagcctaggc ttaaaaacac agcagtggag
     2401 caagttagac atcagaattg aacgatcgga actccaaaga tttttgaaag aaaactttga
     2461 gttctaaagg caaaggagaa tgattttatg tcctacacat tgcaacaaga acatcaaatt
     2521 ctaggtctga ttaaacagcg tagaaaacaa ttacaagatg atcgtgcagc gcttagaaaa
     2581 tccgatgagc tatcagatag acaagctgaa ctaattgctt ctgaacttga ggatttgaga
     2641 atgctagaaa taaaaaatag ggagattaga ttatgaagaa gacagacaca ctttttatag
     2701 gattcatttt ggggttatta gtgattgtag cgcaccaaag tattatcgga ggaagcttgt
     2761 tcgcagcatt gatggtttta atcaatctgc ttgactcaaa agaaaggagc aactatggca
     2821 cgagaagaag cgctaaaaat cggtaaagtg attgctgata attggtgggc aaatagccgt
     2881 tctattattt taagcaagca acatatcgaa aagaaaaaag catggcaaca aataaaaaag
     2941 tgactccgcc gaccaaagca atgagtcact caaaaaatca taactaagga gattttaaca
     3001 tatggaaaat gaactttcca ctctagatca atatttggcc gatcctgatt ggggcaaatc
     3061 gaatttacaa ctggttgcta acaagtacga aaaatcaatt gatgaagcaa ttgttgcatt
     3121 gactgatgca gcttccataa ccgctgatga cagtaaatgg accaagagag acttgggcat
     3181 tctaaaacga ggcgttaatt ggcttgaaga tcagtacaga gaagaaacaa aagagaagtg
     3241 atatgagtgt ttaaaccact aatagattca tactcggcgg tactaaagaa atttaaaggt
     3301 aacgacattg gtgcaacaat caacgaagaa gtaaatatcg aacggctgaa aacgatgtat
     3361 gacggatatg atggcgatcg agtcattgaa attcgcttta ttgatccacg acggttcaca
     3421 gtacagcaaa gaaacttcat ctatgcgctc ataggcgata ttttcatcga tacaggcatg
     3481 ccaacggact tctggaagga attcttctac ttccgttttg aaggtgtcac agggcgcgaa
     3541 ataagcctca aagacgaatc gaatacaacc gtgagtgatg ccaatatctt agcgaatatc
     3601 atcctagatt tcatctttga acatcatatt cctttcaaag aaggatatga gattttacca
     3661 gcgaatcaag agtattactt ctacaaatgc attacaaaaa gagtctgctg catctgtggc
     3721 aaaacaggag ctgatatcga tcactttgac aaagcgctgg gaagacgaaa gcgcaaagaa
     3781 gttgatcatt cagagtacac atttgcagca ctctgcagaa tccatcacac agagaaacac
     3841 aaaataggtg tgatcaattt caaaaataaa tatcaaatca aagggatcaa gttaaaccag
     3901 gaaacaatta aaaagttaag gataggagga taaatttggc tgagataagt tggatcaaac
     3961 ttaaaactac tatgtttgac gatgaaaaaa tacgattaat ccaagctgtt cctgagtcgg
     4021 atgccatcat cgttatatgg attcgattac tagttttagc aggaaagact aacgacgatg
     4081 gtctgatata tatccagagg aacatgcctt ataccgaaga aatgcttgct acattgtttg
     4141 gcaaaaacgt aaatacggtt cgcttagcgc taactacatt ggcaaatttc aacatgattg
     4201 atctaagcag tgatggacta attgccatca gtaattggga aaaacatcaa aatatcgagg
     4261 gtatggataa agtaaggcta aaaaatgctg aaagaaaccg taaatacagg gaaagaaaga
     4321 gacaggaacg tctcaaattg gaaaatgacg ttagcgtgac gtcacgtgac ggtacagata
     4381 aagatataga agaagataaa gatatagata aagaagaaaa gaaaggtaag tattctgacg
     4441 aacacttacg ccttgctaaa aagttgcaaa gtaatttaac tgaagatttt ccaaaagaaa
     4501 tgaacaaagt agatatcgaa aaatgggcag acacaatcag gttgatggaa gaaagagata
     4561 aagcatctat agaagcgatt gagtatgtga tcaattggct acctacaaat gaattttggt
     4621 ttggaaatat tagaagtgct aagaaattga gagaaaaatt tgagaagctc aaattcgaaa
     4681 tcaaagcaga caagaagaat cataaaaagc aaagtcaaaa actacagtac agcaatccta
     4741 gtgaatatga cgacttgcca atttaaaaag gagatgcatc acatggaaag cctagcaaat
     4801 gctatggaga aactaataag aagagtatta gtgcaaagcg gaaaatgtcc agaatgtagc
     4861 gaacctttgt atagttggcg agctaaaaat aaggatggtt cagaacgttg taaaccaaca
     4921 tgcatgagtt gtggttataa agcgttacgt gtgaaagagg atatacagac cgaacggata
     4981 tataacgata gcttaaaagc acgagcgttg agtttttttc aaaatggttc ggtattaaca
     5041 gataaaactt tgtttaaatg caaaatggaa aattatcacg tagtggatca agaaacaaaa
     5101 atcgctttag aaaaagcaaa aagctatacg aatgaggttc tgctgaacca tcctgcacat
     5161 ttcattctat cagggaaatc aggaagcgga aaaagccact tatcaatggc cacagcttgg
     5221 gaaatacttg agcgctcaaa ttatgacaag aaaatacttt ttataagcta tcaagaatta
     5281 ttagagcaaa taaagttttc ttataacaat actgaactga gaaaagaaat tgaaggatcg
     5341 cttatagccg atattaaaac aactgatttg gtggtttttg acgatattgg agctgaatta
     5401 ggtagtgggg tatcaaatag taggcagttt acaaacaaca cgttaaacac gctcttagaa
     5461 gccagacaga acaaggcaac gatcatcaca acaaacttat ctggtcctga actaagagaa
     5521 gcctacggtg aaagaattgt ttctaggata tttaagaatt cagaaggtta tgcgctgaaa
     5581 ttccaacaaa cagcagacaa gcgcataaaa ccagtgaaag gtagtatcgc atgaataaat
     5641 accgtaataa aaaaactgtt catcgaggta tcaagtttga ttctatcgca gaagcagagt
     5701 attacgatct agccttgtgg caagctgaag caaatggctg gaaagtaaaa cttcaggaaa
     5761 gatttgagct gatgccgaaa tttgaactag acggaaagaa gtatcgcaag atcgagtata
     5821 ttcccgactt cacattttat aaaaacggca aacttgtcaa agtcgtagat gtcaaaggaa
     5881 tgcagacaaa agactttaag atcaaggcaa agttgttctg tcatcaatat caagcgccgt
     5941 tgatattagc taaaaaatat cggaatacgt tcaaggaaga gcgtttttaa cgaggtggtc
     6001 catcatgaca acagaagaag tgattcaaat gcgtattcga aacattcagc gtgaaattga
     6061 cgatctagaa cgaacaaagg cagtgatggt caatgaaacg gcgagaaagg caatcgattt
     6121 gcacatagag aacttaagaa gggaaattcg tagattggag gaatgagcgt ggataagaaa
     6181 gcgacaatga aacgaattgc tgaattaacc aagtcagaat cttggcagga agacaaagaa
     6241 atagttgcag aagtccaaaa gctcggtaaa tcaatgtgga ctgaaaagcc taaacggaaa
     6301 acgccgagaa aaattgcaat ctggcatggt gatcgaattc tagtaacagg tacagctgaa
     6361 cagttatctg aaattactgg attaagcaaa aacattatct gggatagagc taggagctta
     6421 tggattgatt caaaaggacg acagtttagg tatgtggagg agagataatg gatctcatta
     6481 cacaatacag tgacatcatc ctcaagaaaa tcatgatgaa gattcagaaa gacaaaaaat
     6541 caaaagaacg agctgaatta gttaagttag aaatggctga aacaggagca ggagtgcgaa
     6601 gtagcaggca ttggaaagca gcagcaaaca ttgaatttta ttacaacgaa attcaaaaag
     6661 ggttcgatca gatgcgtgag ctggatcggc aaacaaattg gagcaagaaa cttcatcaag
     6721 atcgtttcaa atttgtagag aagtataaag aaatattaga agagtatttg aggaggacag
     6781 caaatgataa aaaaactcgt tcaattcagc atggatttat atgatatcga atcaggagca
     6841 acactatctg tggaatcgga ccatctaatc ataaattttg gtggaaagcg ccagattatt
     6901 ttgtgggtag ttgatgatgt actgtttcca gaaattgttc atgatttcga agaatcaaaa
     6961 gcggttgagt ttgaaatagt gaaaaaagta atggaattga ttgaaaaata cgaggaggac
     7021 agcgaatgat accgaagttt agagcgtggg atagagaaaa agacagaatg aaggcagtag
     7081 ctttggttga atttgctgag gcgtataaat caggagaact catttcacgt acaaaataaa
     7141 aaaagtcgga ttcctccgac tatgaataat atttccgaca taagtattat atcataattg
     7201 ggggaatcag aggatggtac ttttcgatgt aaagaaatat gagacaccaa gcgcgaaaga
     7261 tgttgatatg gaacgcacaa aacataatgt cgctgtgttt ctttcagcat atctatcagc
     7321 tagatgtaga gtagggcaac ctcgagaacc aaaagtgaca gcatcttatt ccttggttcc
     7381 accttctaca gctgatcata tatttgaagc agaaagaatg ctgatcgata aagaagaagc
     7441 acaggaagaa tttgagtatc tgcataaatt gtttgttcga ggctattcag cgatacagca
     7501 tccgcataag cctgatgtga ctgaaaggcg caagaagata ttctatgatc gttatatcaa
     7561 tggtctgccc atttatgtaa ctgctcaaag gaataatact agcgaagaat cggttaaagt
     7621 agaatcaaac agaattatca tccaatttgc ttcatcgtta gaactggttg ctttcaagta
     7681 gccagttttt acacttttta taccctttta ttaccaattt ggtttccatt ttataccttt
     7741 tttataccaa tcacttacct attcaacgtt gtattatgat agtgtcgaaa gattaggaaa
     7801 caggatcgac aaaataaaat gtaagggagg aaatctccct catcgtttaa ttaagcttcg
     7861 ataggcagca gcggaaatat taagaataag gatgtgaatt tcaactcctt ctaaattgtt
     7921 cttattatct atcatccgtt gctgtctatt aatttatgta ttggagggta tataaaatga
     7981 agatcgcttc cgaaaaaatt gatcaactaa caattttagt aaaccaaaga atcgaagata
     8041 ttatcaaaga attcgaaggt gaaaagatta agaacataaa gcttacttta atggatcata
     8101 agttcgaatt aaattcgaca aaaaggaaga tcgttgagct tatagaagaa gaaatagctc
     8161 tccagatcaa tgagcaacaa ttagctaatt aatttatgta ttggagggga aatgaatgga
     8221 agtcgaacaa ataaagtatc aaggtatgga tcgggaacgg ttgattgata ttattgagca
     8281 acaacgttta gaattgatcc aaaaagaagc tgtatgtaaa gagtacaaaa aacatttgga
     8341 acaagtaatt gagtatcatt cagtagaaaa gtacagatca gtggtgcaaa aaaatagagc
     8401 aacgagtcct gattctcgtc aagatgtatc gattagtatc aagacgccag tatttaattc
     8461 tgttaaatca gaaaagctag aaagtatctc aactgctttg gtagaaaaag cagctgaaat
     8521 gaactcccta gcaaatgaag ttagtcgtct tatttagtca tatcgataac ttgagagtct
     8581 ttggtgattc cgttttttat agaattaatg gtttgttcag cggaatattt agtcaagtat
     8641 gtttcgctag ttgctactac ttcattgtta ttggacttaa taacgaagta atattggcca
     8701 ttagttgctt ctcttataac aaagtacaaa tttttcacca cctataattt atttcagcgg
     8761 accactcgct gataattaaa attataagct atgtataaaa tttcacaata tcaatttgtc
     8821 gctgtggcgg aaagggtaga cgcaataaag gatgaatggg aaaagtcaat gagtaattat
     8881 ctgaaattta caagtcccct gtttagttat ctaagattat ttgataatat tatttctgag
     8941 gtgatagaaa atggtgggcg tagttaaatt tcttgggttt acatttgatg gatatggtac
     9001 gttaggcgaa tgggtaggta ttattttgat agtgattaca attcaacaaa catcaaagta
     9061 ctactggtat tctaatagtt ctaaaattgt atcaagaata actgaaggaa taaccaaaaa
     9121 agatattact gtcgtcggta aatatgggtt tactataact ttattaaatg aagggaaatc
     9181 tgaaaccgca ataaagtttt gcgggattat aggtaaatat aacattttcc aacgttttat
     9241 cagaaagcat tggtacaaat atatttacaa ataccctgta gttaaaaagc ttattgaaaa
     9301 atatgtggac cacgaaatat cttatctcaa tccaacagac cttattaatc aagaattcca
     9361 gaatattgga tacttagaaa aaggtctaaa aataccaatt gtaaatgaaa gtttagttac
     9421 tgcaatggta gctaacataa agaacagcaa gaaattagaa aagagatata aaaaatgtaa
     9481 aaaattgaaa ttttcctttt tattcttaga atataatggc aaagaatatt atcaagatat
     9541 ttatgttttg cgaggctcga acttatataa agacattaaa aataagattt cagtatgaga
     9601 ttttttaaaa ttcgtagatc actctttgag tggtcttttt attttgcgta aaggaggcac
     9661 gacgatgtat agaccacaat acttagaaca gaagcatgaa gtaatcactg tgcaaaacgg
     9721 taacggtgag atagtacgaa agtatagaag accaataaag agcgatacat ataaacggaa
     9781 ggaaagcaat gaagttattc cattgtatgg caaaagaata gctaagtatt aaataagatt
     9841 gcgaaaggag atgggacatg accgaggaat tctatagatg gttattacag ttgataagag
     9901 aagatcgttt ggttaagttc tatcagtctc ctaaatggcg caggcttaga gagaaagcga
     9961 tgaaacgaga tcactatgaa tgccaagagt gtagaagact aggtaagtat catagagtag
    10021 agaacgttca tcatataaag gaagtcaagg atagacctga cttagcttta gatttagata
    10081 atcttatttg tttatgtgtt gaacatcata atgaagttca tggcagatat cttacagcgt
    10141 tagataaaca agagaagaag atagaaagct tcgctaactt cgatgcaagt gaaaggtggt
    10201 aagtgcatga tcatcaatga caatggcaga gagtatgata cagaaaagat tgaagagtat
    10261 tcatcttata ctcagggatt aattaaacgt ttgatatacg ttcgctatgt aggtattagg
    10321 gatctgttat cagataactg ttgtagtaaa tacaaagtga atcaagtaag agaagcgttg
    10381 aataaagata ataacgtcga aagaataaaa aatgtttttg gatatagtat tgaagagatt
    10441 aattattaca ttgacttcgc tgaagctttc attccgatgg tgagataacc cccccttaaa
    10501 ataaatcgca aattttttgg gggtgatgaa acggaggggg ctgtcaggaa aagagatttt
    10561 ttcgaacttt atcatgaaag gagggctaaa atgtttaaaa acgaattgtc tcaaaatcgc
    10621 tacagagaaa aattacgccg ctctttaata agccaattgg aaagtcagaa aacaaatatt
    10681 gagccattct tagataatgt tgatcgttat atcagtttat gggaaacggc gatatcactg
    10741 gaagaagata tatccgagaa cggcattaga ctggagaatg gtaaaaagaa tgaatcagta
    10801 gcgttgcttg tttctgtcaa caaacaaatg ggattgatgt tggataaact tgccattact
    10861 cctgaattgg taggtgaagc aaatgaatca attcctgagt tataagcata ttgaaaattg
    10921 gttcaaagct atagaagaag gcactatcaa ggtatgcaaa gagcaattat tgctaaaaaa
    10981 ttatctagaa gaaagagtct ttactagaga agatatttac ttcgataagc agatggtaga
    11041 ggattcaatc aatataccag cacaatactt tccattcgaa ttaattccgt gggaaaaatt
    11101 tctacaatgt tttatttatg gtgttcgatg gaaaaaagat aaaacactag tgttcaatag
    11161 atatctttca ttaatgggac gtggtaatgg taaaactggt tttgcttctt ggaacaactt
    11221 ctttctacta accgctaaac acggtattaa aaattatgat attgatatct atgccaataa
    11281 tgaaagccaa gcaaagacta gttttgatga tgtatttaaa gtaattaaag atcatcctga
    11341 tttagataaa aaagtattta aagctacgaa ggaagttatt caaaatatcg ctacaaacag
    11401 caaacttcgt tataacacgg caaatgctag aacaaaagat gggaagcgac caggtgcaaa
    11461 ccgctttgat gaaattcacg aaaatgaaga ttattcaatg ataaatgtgg ctacttctgg
    11521 tggtggtaaa attcgagatt atagagaatt ttatgataca actaatggtc atgttcgtgg
    11581 tggtccgctt gatgacatta tagaagaatc aaaaatgatt ctttctggag aacttggaat
    11641 tgacaaggat ggagcagaat tttctagttt gtttccattt atttgtcgct tggataacga
    11701 taatgaagtt gatgatcccg acatgtggga aaaagcttgt ccaactatta attacaatgc
    11761 agatttaaaa cggaaaatgt ttcaagaata ctctcaaatg caacgtaatg ctggtttaag
    11821 acttacgttc atgaccaaac gaatgaacag acctatggaa gatacacgat ttgctgttgc
    11881 ttcatatgat gatgttctgc atacgaaaga aaaagaattt cctgaaaaaa tggatgaagt
    11941 gataggaaca gtcgattttg ctgatagacg agattttgcc agcgttgggt tgctaggaaa
    12001 atacgataaa gatgtttatt ttacacaaca tacttttatc cacgaatcag cccttcgatt
    12061 acaaaacatc aaacgagagg ttatagatat ttctatagat caaggaaaat cacagatcgt
    12121 tcatggaaaa aatatagaag ctgattatat tgtaggttgg tttcttgaaa tgagtaataa
    12181 atattatatt aaaaaaatcg ctatggatat gtaccgtgca aaaatattga agcccgcttt
    12241 agaagaagca ggttttactg tggaaattgt tcgaagcgga tctgttacac atggtatgtt
    12301 aaaagatctg gttgatgacc tttttattaa tcaacgttta ttttttggtg acgatgcgat
    12361 tatgcgttgg tattgcatga atgtatatga agagcatatt tctaatggaa atatacgcta
    12421 tgaaaaaata gaacctgaaa ctagaaaaac ggatggcttt ttttcattcc ttcatggttt
    12481 gaatttttta gatgatattt atgattctgc tcctgtaaca gtcacaaata gctcagtaga
    12541 aaatacagga actggattta ctcctctagt attctaactt gaaaggaggt gagaaagtgg
    12601 ggatttttca aaaggcggta ggatacttca caaaaaaagc aacggttcct ttagaagaat
    12661 acttttgtaa attgcaagtt gattttgtgt atcgaaaatt tgcgattgaa acttgtattg
    12721 atttgattgc aaatgcgatg agtaaagcgg aattcaagtc atatgaagat ggaaaaaata
    12781 aaaagaatga tctttactat aggctgaatg tagctcctaa taagaaaaat aatgcaacag
    12841 aatttagaaa aaaactgatc aggagattaa tattctacaa tgaagtattg atcgtttctc
    12901 cgtctaataa ttctagcgaa atatttattg cggatagttg ggatgtcaca gaatatgcat
    12961 tgaaagatga tgtgttttct caagtgcaaa ttaacaacat agtccttgat agagaatttc
    13021 tagaaagtga tgttatctat ataaaatacg cagatcaaca aattaggcaa ctagtcgatg
    13081 cgtattatca agcgtatggg aaactcattt ctagtgccat gaatgtttac aagcgctcta
    13141 acgctcgtag atacgtactg aaagggaatt tattccgacc gcaagacaat acaacacaag
    13201 atcaaatcaa taaaatgatg acatcacaat ttaaggcttt tatggaagct gataatgcag
    13261 gtgcggtatt tcaattacaa aatgagtaca cattagaaga tttcagcgga aacttccaaa
    13321 gcaattcaag agatataaaa aacttaatag acgacatctt tgagatgaca gcagcagcgt
    13381 ttcacgttcc gaaaaaccta ctaaagggag acatgagtgg gttatcggat caagtggacg
    13441 cttttttaat gttcgaaatc ataccgattg ctgaacttat tcaggatgcg tttaacgcta
    13501 gtctctatga agtagaagaa tacttgtcag ggaattttgt acgtgtggat acaactatga
    13561 tcaagattac tagcttcaaa gatttggttg acgctattga tgtaggcatt agaaatggag
    13621 tatttacaat caacgaagga agagagcgcg ttggaaatga tcgctctgat aaggcgatgg
    13681 cagatgaaat atttataact aaaaacaatc aacaagtatc gaaaggaggt gaggcgaatg
    13741 acgacaatga aaacatttct agcagtaaag aatgaaggcg cagtaccgca aatttttatt
    13801 cagggattta ttggttctag ttggttcttt gaagggaata ctgacaaggg aatcaaaaat
    13861 attttggata gtctaggtga tcaagaagaa attgaagtag taattaattc aaacggtgga
    13921 gacgtatttc aagggattgc tattgggaac ttacttaagt caaataaagc aaaagttaac
    13981 gttgtgatta acggcttagc cgctagtgct gcttcaatta tcgcaatggc tggcgatact
    14041 ataaaaatct acaacaatgc acaattgatg attcaccgcg cttccacata cggagaagga
    14101 aatgtcgatg acttccgtac gattgctgac caactggaat caattgataa atcggtaaag
    14161 gcttcatata aaacacgatt caatggcaca gatgaagcat tgcaagaact tcttgaaaaa
    14221 gaatcgttta tggatgcaga aacagctttg agttatggat tggtcgatga aattatcgat
    14281 gcagaaaata gctcaggtac tgaagctaaa aaagaacaaa gcgttgaaga aattttgaat
    14341 gacgttaaag aaaaaagagc agaaaaaatt gctgcattta cagcagcatt aaataaaaca
    14401 tttggacaag gagatgtaaa ataatgacag ttaaaaattt aaaaggtgta acagctgcaa
    14461 gcgaccaatt gatgaaagct tttaaagatg gtaacgaaga atcttttagc gcagctatgg
    14521 taagcttatc taaggaaatt caggataaaa ttttagaaga agcaacagca aaaaatcaag
    14581 atcaattagt attaatgaac cgtggtcagc gtgtattaac tacacaagaa acaaaattct
    14641 ataacgaagt agtgaaaaac gaaggttttg caggggtcga agaattagtg ccagctactg
    14701 tatttgaacg tgtatttgaa gatttagaac aatctcatcc actattgcaa aaaattactt
    14761 ttgttaacac aactggtgta acagaatgga ttgtgtcacg tggagtcaat ccagcatggt
    14821 ggggtaaact ttgcgaagct gttaaaaaag ttttagataa tggctttgat gtaattaaca
    14881 tgaagcagtt caagctatca ggttatattc ctgtatgtaa ggcaatgctt gatttaggtc
    14941 cagtatggtt agatcgttat gtccgtactg ttttagtaga atcattgaga attgcattag
    15001 aacaagcaat tgttgatggt actggtaaag atatgccagt cggaatgatg cgtgacatga
    15061 gcaaacaaac tagcggagaa tatgctgaaa aaaaagcaga acctattaca gctttagatg
    15121 ctgtaactat gggcggtttg atggcgcgac tatcaaaatt caatatcgaa ggtgtgaatg
    15181 atccgattta tcgtaatgtg aatccttctg atgtggtcct aattgtgaat ccaactgatt
    15241 actggtctaa agtattccca gctaagactg tactaactgc taatggagaa tacgtacaag
    15301 tattgccagt accagtttca gatttgcagt caacggctgt gccagaagga aaagcagtta
    15361 ttggggtagc ctcagattac ttcatgggtg taggatctac actaaaaatt gaagcttcag
    15421 atgaatacca ttttgttgaa gacgaacgca tttatctagc taaacaatat gcaaacggtc
    15481 aacctaaacg taacgatagt ttcattgtat tagatattag cgctttggga actactacta
    15541 caactacaaa accaacaacc acaacaacta caacacaagc gtaggtgatc agaatgaagt
    15601 atattctttg tcagccggca atcaatcggt ttaaatggga gcttgaagtt tgtttaacta
    15661 atctgaagaa actaggaatc aaagatatcg tattgctttt cagcagacac gatgatcaga
    15721 ttcctatttt ttttgagaag gaatatggtg ttgaagttca tgtgtacgat gatctgcggg
    15781 acgacaaaga gtatattcct tcgattaaac catatttatg gtggaaatat ttagaagaag
    15841 atcattcgcg tgaggacgac cgatatttct atatcgattc ggatgtcatt ttcaataaaa
    15901 gaattaattt gcgcaaattg ccttctaaag atgatgtttg gtattgtagc gactgctgta
    15961 gttatctaag tcttgattat attagaagct gtgaaaacgg agaaaatatt ctaaaagata
    16021 tggcaaacat tgtaaatgtt acagtagaat ctttggaaac tataaacact aattcaggag
    16081 gcgcacagtg ggttattaac cgccctaaag cgaattattg gaaaaaggtt tatctggatt
    16141 ctaatcggct atatcgctac cttagagggc aaaaaacaaa tatacaaatc tggacagccg
    16201 agatgtgggc acagctttgg aacatgatgt atttcaatat tggtcctaaa gttcacgagg
    16261 aattagactt ttgttttgct actgatccaa tagaaaaagt taaagaagta aaaatcttac
    16321 acaatgctgg agtaacaaca aacgatgaag atttattttt caaagggaga tacgtgactt
    16381 ctacgccttt tgatgaagat ttatcatttg taaacaagaa aaaatgctct tacgcatatg
    16441 ttaaagcaat taaggcggtg gttagatgac gcctgaacaa gtgactgaag aattgctaat
    16501 agctgtgaag gataatattt acgttacctg gaacgaagaa gatgagtcaa ttaaaaagat
    16561 gatagctaaa aatgctgttt atcttcaaag taaagtgagt acaacacttt ctttttctcc
    16621 tgaaagctta gaatacggat tgctaatcga aagatgtaga tacgactgga atcgtgcttt
    16681 agatgagttt gaacaaaatt tcgctagtga gttattaggt ttcattcaac attatgcgct
    16741 acaagaatat attgcaggtg atgtgaatgg cgaataatcg tagactcgaa gaaacattca
    16801 acgatggttg gttaaagatt ttgacgcaaa ctaccaaaag aaatgaacta ggaaaaaaga
    16861 ttggtgtaga agatacagaa atcacttctt taaaatttag aaatctttcc atgagagata
    16921 gtgatataac agctatggat gcgatgggat cgaaattaac taagaaagta aagactccat
    16981 ttcatccaat cgccaagaaa tttaataaag atcaatattt tatcgtaatc gatagtatgc
    17041 gttacaacgt tatctatgcc gattacgata atttttatat ctatttttat cttgaaagtg
    17101 tgggtgaata tggtgattga taattctaaa gaaaaagaac gtttaaataa gcaaatttct
    17161 gctatcaaaa cttccttaga agagcatttt ggcctcaaac tctttcaaga ctccgtaggc
    17221 gaggatgagc tacctgatga ttttaattac ttcattctcg aaacaggaga aatagaaatg
    17281 atcactgagc caaaatatag cgtgggtcaa aatctatatc taactttcta ttcagaaaat
    17341 agagaagatt taacaggaga ttcactagat attatttcat tgattcaaaa tcgttcgatt
    17401 cgttttcaga gaatggatcc caatcattta aaactagaga accaagatcg ctatatcgat
    17461 caattggtat ttacgtttag acgattattg aagagtgatt gtcatggcta aaaatagttg
    17521 ggagctaaaa ataaatggac atgatgaact tcttgtgcgg atggaacgct attcaagcga
    17581 gagcgaacga ctgattaacg aagcattgaa atcaaaaggt tcggctattg cagtggatag
    17641 gattacagaa aaaattcctg tttctgaagc agatttaaga agagggcacc aacacgcaaa
    17701 aaatagtcgt ccacttaaga ctcaatacat taatttgggt ttcatcatta gacctacaag
    17761 aaaatttgag tatttaaaat atcctgattt ggggataggt acttctaaaa gaaatcagcc
    17821 ggacgaattt atgagaagag gattaggtct tgcacttgat ccaattacag aacttctgat
    17881 tcgtcaattc gataaattaa ataaatagga ggaacaacaa tggctaaaac aacaacagta
    17941 acaacttttg acaatataag tattaaaaga atttctttta attttaagaa tgcaacaaat
    18001 gcaatttcaa ctgattgtaa tggacaatta gatggcgaaa cagaaatgca gagaattgta
    18061 aaaaaatgcg gttcaacaga agtaaaatcg aaatctaaac caatcaatat gacggtaaca
    18121 attactgcac atgtaccgat ggaagtttat cgacgcttca atgggttgaa acaagatgaa
    18181 cgtattaaat cgggaattta ctcttatggt cctgattccg taggggaaga tttctctctt
    18241 gctgcagaga tcgtggatga tttcgaagaa aaaaataagc tgattggtat gttagcatgc
    18301 acttcgaata caggattaac attctctatt gaaaatggtg cggatgaagt agctgcgtta
    18361 gaactagaaa caaaagttat gcaagatgaa tttggtaaat tttatcatga agcaattgtt
    18421 gcagaacttg aagaagactt aacagatcaa tggatgacga atctatctgc tgatgtgatt
    18481 aaaaaaactt cagttgtgac aactacggcc actcaatcac agtaaaaaaa acggaggtag
    18541 cgaaatgaac gaagattact caaaaattga actaaacgat ggaacaattt tgaatttaga
    18601 acctaaactg aatatcaaga aattattgat gatcaataga gattttaaca cagacgagtt
    18661 tgcaaaaatg actgtgggaa aaggatccat ggatatttct gttattcaag gtgcaaaggc
    18721 tgtgtatatt gcttaccgcc aagcgaacat gactgattat atttcattcg atgaatttat
    18781 cgataaatgg gattttgata tggctactgc cagctatatt tatcaattga tgatgttcaa
    18841 acaagcacgc gatgcttatc aaaaagaatt tgaaaaagca aataaggaaa aaaagcttca
    18901 aaagtaaaaa tgccaaagct cttagttgaa acgtgggtcg atgtctattc gatgttgacc
    18961 gacgtttttt ctatgccttc agatttggtt ttaagcgata tctgtttaga tgacattttg
    19021 caaatggctt acaacaagag tgcttatgaa ggatggaaaa actatgcaat aaaccaatcc
    19081 cagaaaaact aaagaaagga ggtaaaaaat ggctaaaaag agaacagaag cagaagtaac
    19141 attcatagct aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg ctgaattaac
    19201 taaaaataga gcagaattaa aactagaaca agctcaatta caacagactg gttctgaatc
    19261 agacaagtta ggaagtaaat tatcttcttt agaaaagcag tatgaattac aaagtcaaaa
    19321 agttgaagta actagccaac gtttagccaa tgccaaaaaa tattatggag aaaattccac
    19381 cgaagttcag aaacttgaga gagaactgat taaccaacaa acagcgcaac aacgtttgtc
    19441 aaacgaaatt gataaaacga gtaatgcact agctcaagca aaaggcgaaa tacagacgta
    19501 cgagtctaca atgcaacagt tggatagtga acaaaaaaat gttcaagcta gtgcttctct
    19561 gattgaatcc gaatacaaaa aatggcaagc aactgctggt caatcagctt ctgaatccga
    19621 gaaattagcg aaagcccaag aatatgtttc tcaacaatct gaaaatgcag agaaaacgat
    19681 agatatcctg agacgacagt tagaagctac acagtctgag tttggcgcta catccacaga
    19741 agcaatgcag atggaggcga agcttaatga tgctgaacgt gaatttgaag agttaggaca
    19801 agctgctaaa aatgtagata caactaactt ggacgatatc ggaagcaaaa tagatatgaa
    19861 taatctaatg gaagcttctg acgttttaag cgacattggc gataagctta cagaattagg
    19921 gaaacaagca gtggactctg ctaatagtgt aggtagttcc cagagtaaaa tacaagctaa
    19981 ttttggtttg actaaacaag aggctgaaga attaacgaat gtagccagag acatttatta
    20041 taaaggtttt ggagaatcgt tagatcagtc cacagatgca ttgattttgg taaagcgtaa
    20101 tttaggcgat ttaaataatc aagatttaca aaatatcacg gaacaagcta tggtcctaga
    20161 aaacaccatg ggcgctgata tggatgaaac gttacgtggt gtaaatggct taatggtcaa
    20221 tttcggcttg agcgctcaag atgcaatgga tttaatggtt tcgggtactc aaaacggttt
    20281 agataaaacg cacgaattag gcgacaatat ggcagaatat agccaattat ggagtcaaat
    20341 gggatattca gctgatgaaa cgttcggaat gcttcaaaat ggtttagatg cgggtgctta
    20401 taaccttgat aaagtcaatg acttagttaa ggaaatggga atatcgttaa cagatggtcg
    20461 atttgagcaa aacatggata tgtttagtga aagtactaga aaagcttttg aagagtggaa
    20521 aaatggcgga ggaacacaaa aagacgttat taattccatg attcaagatt ttagcaatat
    20581 ggatggtcaa tacgaccaat taaataaagc ttctacaatt tggtctgcac ttggcgaaga
    20641 taacgcgatg aaagttgtcc aatctttgac tgatgttaac catacatttg atgatgttag
    20701 tggatctgca caaaaaatga atgaagattc tactactccg ttacaagaat taaacggaaa
    20761 aatagctgaa ttaaaggatt cattagctcc tataggcaac acaatcatag atgcactcga
    20821 accagtaatt gattttctag gaaagatggc tgatgcgttt aataatcttc cacaaccagt
    20881 acaggattat gccgtagcga ttggcggatt gactgctgca tttactttat taatgccaat
    20941 aatagttggc ttcatggctc taggtggtcc tactacatta ataataggag cagttattac
    21001 tgttattgct ggagttatag caattataaa aaactggggc gcaattactg actggtttaa
    21061 gggaatatgg agtaaattca ctgattggtt gggtggtact tgggaaagta taaaagaagg
    21121 tgcctcatca gtttgggatg gagttaaaga aacctggtct ggatttgtag attgggttca
    21181 agatatttgg caaggagttt ctgattggtt tggagagcta tggagcggat tagttgaagg
    21241 agcttccaac atctggcaag gagtccaaga gacttggcaa gcattcgttg attgggtttc
    21301 aaatatttgg aacggagtca aagaagtatg gtcgattatt tgggcagaca ttgtaggaat
    21361 tgttcaaata ccatggacct taataacgtc attgattcaa gccggtatta atattatcgt
    21421 gggtattttt gatgtagctg gacagttatt aggcgcagct tggcaagctg tttggacacc
    21481 tatttctgat ttccttaaaa acacttggga tactatgaca caatgggtaa gcatcgcttg
    21541 gaatggaatt gtaactacat tccatactat atttgatcca gtagtggcat ggtggaatgg
    21601 tatatggaca gctattagta ctacggcttc aaatatttgg aattcaatta gtgcaacagc
    21661 ttctagtatt tggaacagta tcaagaatac aatcactagc ttggtacaag cagctgctac
    21721 agtaattcaa aatatttggt caactgtatc tagttggtta ggtggaattt ggaattcaat
    21781 cagctctaca gcatcaaata tctggaatag cgtgactagt agtataagca atgctataaa
    21841 cgcagctaaa agtgccattc aaagtgtttg gaatagtata tcttcgtgga tcagcggaat
    21901 ttggaacggt atcaaaaaca ctgctttgaa tctttggaat ggaattacaa gcactattag
    21961 ctctaaagta aacgatggaa aaaatgcaat ttcaagcggt tggtccaatc taacaggtat
    22021 tgtttccgac atattcaata atgttaaaag tacaattgct aacatttggg aaggtatcaa
    22081 aaagactgtt agcgctccaa ttgattggat cagagataaa atcagtagta tctttgataa
    22141 tttgaatatt tctataccac atattccgtt accacatttt aaattgagcg gagaattcaa
    22201 tccattgaag gggaaaatcc caacgttggg tgttgattgg tatgcgaaag gtagtgtgtt
    22261 taattctccg aatattatcg gtgtcggcga agcaggacct gaagcagttt tacctttgaa
    22321 aagatctgtg ctgcaagaaa ttggtgatcg tatcttgagt agcacatcag tttcatctag
    22381 ggcacaaacg attcaacctg tgaataacta cgaattcaat ttcacaattg atggtaacgc
    22441 agatgaggtt actatgaagc aaacaactca acaaatcatt gatagcatta caaaagttca
    22501 aaatgataat gcttcggcat ggcgttaaac aggagagtat ttctcctgtt tttttagtat
    22561 taaaaaggat gtgaaaaaat gactgattgt atacattcta taatcgatgg atttcctgat
    22621 tatttgcata aattggcttt agctgaaaga ccaaccatac cttctccaaa aagacagaga
    22681 gttgaaactt ctgttttagg aaggttaggt ggcttagtac aagattactc gtttgaagac
    22741 atgtcgttta cattgcacta taactattta gaggatgtgg aagaccatca agcgttcaag
    22801 caatcgtttt atatcatgcg tcattggtta aattatgcaa agaaattaga attctctgat
    22861 gatcccaacg tctattatgt tatccagact atcgatattg gggatgcaga aaacgatatt
    22921 gttgaatggg gagagttcga tgtaaatatt actgcgaaac cattcgcaag agttcaagaa
    22981 gatgtaccta taaccgtaga taaaccacag tcatttaact tgctgaataa tagtttagaa
    23041 gaaagttttc caaagattat catcactcct tcagctactt catgccagtt catcttaaat
    23101 gattatgtgt ttagttttga aggcttagta gtaggaactg acgtagtcat tgatagtgat
    23161 ttgatgcttt gctacgaaga gcaatcggac ggagatattt tagatcggtc caacaaaatg
    23221 aagaccatgc aatatccgac attgcaagtg gatattaatt attttaattg tactggtttg
    23281 agcaaaatac aaatttatcg taatgggtta aggtaggtga aatagatgat cgataattta
    23341 ataactattt acgataaaaa cgacgcgaat aatttagctg aacatttata tgatacgcaa
    23401 ggtttaggcg ctttgtcaga ctggttaaca gctactgtta gcaataaatt aaacggagcc
    23461 gagatatttc agggtactta tccaataagc ggaactaatg cagatttgat tgtagaagga
    23521 cgtattattc agtgttatgt agatgaaaat cgagcaaaac agcgtctacg gatttattat
    23581 gcaaagactt ctgtaatagg caatacgata gaagtaaaag ctgaacctat tttcaatgat
    23641 ataagaaaat cggtgttgaa taaatatgac agtggaacag aaaagatcac tgctagtcag
    23701 gcatggcaaa acgcaaaaac tttagcgaaa ccagttattc cttcacagtt ttctttctcg
    23761 tcattagtag atacgcttgc taatgtgaag atagaaaagg cgaatttttt agaattcttt
    23821 ggtggaaaag agggatctat tctagatcga tttcatgggg aatttctaaa agataataac
    23881 acattacgtc atgaaaaaag gctaggcacg gatcataaaa tcaaagcgat ttatactaaa
    23941 aacttaactg gtcttgactt agagattgat gctcaaagtg ttttagttgg agtttatcca
    24001 ttcattagca gctcttcaga aggagaagac gagatcactc taccagaaga agttattttc
    24061 acggattacg tggatgatta tcctgctgga tatgtttctt ttgttgattt taaagacaaa
    24121 gcgactgatg tagccacatt aagggaagct gctaaagact ggttgaaaac aaacatagat
    24181 aaacaaaaac cacaagtgag tggttcgatt gaattagtac cattgaggca tcaaagaggc
    24241 tatgaaaaat ttgttgatct agaaaaagtt tcgatgggtg acggagtaga tgtgtatcat
    24301 ccacagttaa aagtgaatat gtcagcgaga attgtggaat atacgtttaa tgttctaacc
    24361 aattcatacg ataaattagt tgtaggaaac gtcaaaacaa acttcttaga aaacacagag
    24421 aataatgtca gcaatttgat taatgatgcc attgatcaat tgaaaaacgg tggcgaaatc
    24481 agtgatttac tcaatgatat tgtagatcat caaactgata tgattactgg tcaaaatggt
    24541 ggttatgttt tattagatcc taaagaagcg cctagtcgta ttttgattat ggacacacca
    24601 gataagaata ccgcaaggaa tgttttacaa atcaacaatg ctggtattgg tttttctaaa
    24661 actggcatta atggaacata tgaaacggca tggacgttag atggcggatt caatgcctcg
    24721 tttattacgg ctggtgaaat agtaggaatt actattagag gtactacatt aattagtgat
    24781 ggtgctgatt atagaacaag tattgctaat ggcaaaatga cttggtactc aaaaaaagtt
    24841 aacaaagata ttatggagct agaagcacgt gattatgtaa gtgctgatgc cggtattgta
    24901 tcatacacca tgaaaactgg tggtggtttc atgattagaa atccacaggg taacttggtt
    24961 tttagtacgt gggataatgg taataacaga ccgtttctat cttttggtgc gcccaatttc
    25021 aggtatagca atgctagtta tgtaacttct ggcgacggta gttctttaag cattaatggt
    25081 agtgcgggta actcatggga atttaaggta gctggtagga ctatgaaatt tactagtgat
    25141 ggtatgctaa cgttaccagg ttgttttttt ggttcatggg aagatgggaa acttgctagg
    25201 tttgaacaat caacggtaca agtatataaa gattttactg ttagaggtac taaaaactca
    25261 actgtaccaa cagaacatta tggacaacga ctattgaacg cttatgaaac tccagaatat
    25321 tatttcgctg attatgggga agccgttaca ggtgacgatg gtaaagttcg tgttgatatt
    25381 gaccccatgt ttgctgaaac agtaaatcta agtcggtata tgacacatgt gacacctaca
    25441 gaactagttt tgtgtgctgt tactcatgaa gatattgacc atttcatcat tgaaactagt
    25501 aagccaaacg tattagttag atggaattta gtggcacacc gtctagggta tgaagatatt
    25561 agattaaaag aggatacagc atatgatagc acagtgcttg accaaaaacg tttttaaaac
    25621 gaagacaagg aggtatataa atggctagca gtttatataa tttggcttta gatttcagca
    25681 aagaattaaa ctacaccaaa gctattatgg ctcgtcaagg tgataaaggg attacggtga
    25741 cggttaaacc gtttctaaat ggcttgcaga tggatacgag tggcggaaca tttactttaa
    25801 aaggaacaac accatctaac cgttacgtag ataatgttgc aactagtgta actagtgaag
    25861 aagtcacgtt ttctcttgat ggcacattta tgagtgaagc aggatattat aaacactgct
    25921 acgtagaata tagaaaagac aatcaaattt taacaacgca agatatcatt tttttctcac
    25981 taggagtgtc tgacatttcg caaggccaag ccgatgaata tgtttcgcaa ttagaagagt
    26041 tgattcgaaa gtacaacgaa acttttgatg cttttatggc tgaaatcaaa ggtagagtgg
    26101 atagcttaaa tcaacagatt actgatttaa ctggtcaagc taaaacgcta caagacaagt
    26161 tagatgctct gaaagaagaa atttctaagt taggtaactt acaagtgatg tacagtaaca
    26221 gcatcgactt cgggggatat gattatagcg gtaagccgaa tttaatgtcc aaactaaaat
    26281 cgagcgattt taacgttggt taccacgggt cactaacttt ggataacgaa aagttacatt
    26341 ttacttctga tggtacagga agcattatta tgtttacgcg tattaataca cctcagctta
    26401 ctagtgggaa aacctatact ctgagtgcga aagttcgatt tgatgaagga actacaggag
    26461 ctattgataa attacgtttg gtgtatcgta catcaccagg agaaaagata ttattggaag
    26521 caaatagtac aaatattaca acagatgatg tagggaaaga aataacaatc aaaggtacag
    26581 ctaacgttaa ttatcaaatc acaaatttag atcgatttta tatgagtatt agctttgttg
    26641 acagggataa aataaatggc ggatttaagt tgtacgacat caaaatcgaa gaaggctcaa
    26701 cagccacacc gtaccagcct aatctacttg atgcaccgta ttatttgagt aaggtggctt
    26761 tgggtgagaa tctaattaaa ccagaatcac aacaaccagt tactaatagt aactatctta
    26821 ttaacaccta taacattaaa ccaatggtaa aaggtaagaa gtataccatc acacttgaag
    26881 gaactaagcc agcaacacag gtttttagac catttttcac acgagcaaca ggagatgcat
    26941 gggaggttgg tgacttacaa ccagtagaag gcttaactaa tgtgtggtct aaaacattta
    27001 cagcagcaga tgactcacac cctactaccc cacaagtgca gatttatcaa gtaccaagca
    27061 caagtgtagg acaatgtaca attaagtggt taaaactaga ggaaggcaat actcgaaccc
    27121 cgaatattag tgagtataaa tatcgtggta ctggtatgcg tgattcaaac aatccaaaag
    27181 attatgtttg ggatctagca ccagaatatg tcgaagataa tttggccaca gatattaaaa
    27241 tttctgaaat tactggtaaa gcaaacaatt ataccgatgg gaaagtatcg gagattaatt
    27301 cgcagttgac tgcttcaatt aatgaagtag acaccacagc taaggatgct caaacaaaag
    27361 cgaatgctaa tgcgactgct atagatgaat tagacaataa gatcgatgaa cgcattaatg
    27421 atacagctac taccacatta acagttacaa acgggaatac cggatcagca aagctttatc
    27481 gtgaaggaaa aacagtttct atatattttg tggctttaaa cggaaaacgc agtggtggaa
    27541 atgattcaac gatactaaca attccagaag gctatcggcc accaattagt tttgagcaac
    27601 tggttggctc gatagaccgt tctactttga acagtgctca gttatctatt ggtgcagatg
    27661 gagccattaa atggcgaaga aactcaagtt atggatcgga ttataccttt gcaattactt
    27721 acacgattta gaaaagcgtg aatcgatatg aaggcagcat atagaccaat tgaaccttac
    27781 ggattcgagc aaatcattgt gaatgatgaa gaacatttac cggaagaatg cacagaagtc
    27841 gaaccaccga ttccaaattg gaaaccgaaa ttcaattact gggagggaaa taaatgaaaa
    27901 acatttggaa atatggacgt actggcggag agtacgcagg aaaagtattg gacgacatgc
    27961 ttgtatccgt tccttacaca gatcagccac cgcttgaagg aattcgtgct gatggcgaac
    28021 cgttaacaat cgctgatcaa atgtttgatc ctaaattgaa tcaatggatt attttagcga
    28081 acgcactaga tcacaacgat ttaaacaatc tcaaagcaat gtatgagtcg ttagaaaatg
    28141 agaacggcga tttaaaacag atcaatgcca aactcatgct aagcgatgta gcaattaaac
    28201 aggaaaatac tgcattgaaa gaaaaagcgg atagtttagc acaaatcaat tcaaaaatga
    28261 tgcttgcttc gttacaaaat agcaaagaca tttcagaaat taaagagcaa ctaaatccag
    28321 cttcaaaggg aggtgagtag tatgtttagt tttagcgatg tgaaaatgat gtatgattgg
    28381 ggctgtttca ctaacgaaca agtaatggtt ttcgttccgt tgtgcattac tgaagaaaaa
    28441 gcagataaaa tcattagcaa agaagagagc gcatcttaat tgatgtgctt tttattttga
    28501 ttcaaggagt tgtcacatga ttaatttagg ggaatgggga gcgatagcag gatcaataac
    28561 cgctatcgtt tctttgattt tattagtaat aaaaccaatt actgcatctt tctcgaagat
    28621 tactgagact ctttcaaaag taagtcacaa tttagatttg ctgactaaag atttagaatc
    28681 gagcaaatca gatcgattga tgattcatga agaactaaag aaacacgatg aaagattaga
    28741 tacacatgca gaaaaattgg tagaacacac gcaacaaatt aaaactttat ttagggagag
    28801 aagaaaatga ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc
    28861 gcactagcta ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc
    28921 tgtgttgtca tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat
    28981 cgaacctaca aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa
    29041 ttactattac tgcgatgagc ctgttaacgg ctcttttttt attacccatt aatacgtttg
    29101 cttatactat taatgacgag tataatttag cgccgaatca aggagactcc agattagcaa
    29161 ttcctaacaa aattattttg cacgaaactg gaatagatgc accagcaaga aacgtagccg
    29221 ccaacatgaa aaataattat aacggaagca attcttatac tacagatgtt attggtgacg
    29281 gtgggattgt ttaccgtgtg ggtgagcaag gatatgtttc gtggggagct ggtaacgcta
    29341 atccttatgc gcctgtacag attgaattac agcgcacata tgataaagca ttgtttgaaa
    29401 aaaactatcg agcttatatt gaatatacaa gagatagtgc aaaaaaatat ggaattccat
    29461 tgactcttga tcaaggaact tctttattta caaaaggaat catttctcat ttgtgggtga
    29521 caaattatgt ttgggggaac cacacagatc catatggtta cttatcgcaa atgggagtta
    29581 gcaaagaaaa gcttgcttat gatttagctc atggatttac cgatgaaaat ccaacaactt
    29641 ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct acgctgacag
    29701 atggaacaaa tcacgcccac attgatcagt ttggggaaat cgaaaacgca aacttgcatg
    29761 tggctggatg gcacattgct aactataaat acgagtatat tttcattatg gactacaata
    29821 ctgggaaaga attagctcga gtaagagctg atggaattta tagaccagat gtaaaccaag
    29881 cttataatac ttctggaaat gttggttatc atgtatcttt caatatgcgt aattttccta
    29941 gtaagaaagt ctatgtaatg atgcgggcaa cgaatgatcc agagggaaac actaaaggcg
    30001 gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga taaaaatagc
    30061 tcctcgttga ggagcagtac ataactatat tgacaactat aaaaattatt cgataaaata
    30121 gtgatgttat cgcatatctt cactatcacc cat
//
LOCUS       DAFFSV010000021        30101 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFFSV010000021 DAFFSV010000000
VERSION     DAFFSV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.021"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(16..594)
                     /locus_tag="LR110_000862"
     CDS             complement(16..594)
                     /locus_tag="LR110_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL4821862.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(671..1201)
                     /locus_tag="LR110_000863"
     CDS             complement(671..1201)
                     /locus_tag="LR110_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBL4821863.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1205..2065)
                     /gene="rlmB"
                     /locus_tag="LR110_000864"
     CDS             complement(1205..2065)
                     /gene="rlmB"
                     /locus_tag="LR110_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBL4821864.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2058..2453)
                     /locus_tag="LR110_000865"
     CDS             complement(2058..2453)
                     /locus_tag="LR110_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBL4821865.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2450..3862)
                     /locus_tag="LR110_000866"
     CDS             complement(2450..3862)
                     /locus_tag="LR110_000866"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBL4821866.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3859..4395)
                     /gene="cysE"
                     /locus_tag="LR110_000867"
     CDS             complement(3859..4395)
                     /gene="cysE"
                     /locus_tag="LR110_000867"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBL4821867.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4481..4720)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4717..6177)
                     /locus_tag="LR110_000868"
     CDS             complement(4717..6177)
                     /locus_tag="LR110_000868"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBL4821868.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6347..7504)
                     /locus_tag="LR110_000869"
     CDS             complement(6347..7504)
                     /locus_tag="LR110_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBL4821869.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7538..8908)
                     /gene="radA"
                     /locus_tag="LR110_000870"
     CDS             complement(7538..8908)
                     /gene="radA"
                     /locus_tag="LR110_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBL4821870.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8971..9498)
                     /locus_tag="LR110_000871"
     CDS             complement(8971..9498)
                     /locus_tag="LR110_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBL4821871.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9728..10534
                     /gene="proB"
                     /locus_tag="LR110_000872"
     CDS             9728..10534
                     /gene="proB"
                     /locus_tag="LR110_000872"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBL4821872.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10531..11778
                     /locus_tag="LR110_000873"
     CDS             10531..11778
                     /locus_tag="LR110_000873"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBL4821873.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11854..12198
                     /locus_tag="LR110_000874"
     CDS             11854..12198
                     /locus_tag="LR110_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821874.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12315..12641)
                     /locus_tag="LR110_000875"
     CDS             complement(12315..12641)
                     /locus_tag="LR110_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821875.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13256..14458)
                     /locus_tag="LR110_000876"
     CDS             complement(13256..14458)
                     /locus_tag="LR110_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4821876.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14575..15090
                     /locus_tag="LR110_000877"
     CDS             14575..15090
                     /locus_tag="LR110_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL4821877.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15289..15735)
                     /locus_tag="LR110_000878"
     CDS             complement(15289..15735)
                     /locus_tag="LR110_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL4821878.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15782..17260)
                     /gene="msr(C)"
                     /locus_tag="LR110_000879"
     CDS             complement(15782..17260)
                     /gene="msr(C)"
                     /locus_tag="LR110_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL4821879.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17614..18243)
                     /locus_tag="LR110_000880"
     CDS             complement(17614..18243)
                     /locus_tag="LR110_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL4821880.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18227..18691)
                     /locus_tag="LR110_000881"
     CDS             complement(18227..18691)
                     /locus_tag="LR110_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4821881.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19178..20446)
                     /locus_tag="LR110_000882"
     CDS             complement(19178..20446)
                     /locus_tag="LR110_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL4821882.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20677..21414)
                     /locus_tag="LR110_000883"
     CDS             complement(20677..21414)
                     /locus_tag="LR110_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL4821883.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21481..22050)
                     /locus_tag="LR110_000884"
     CDS             complement(21481..22050)
                     /locus_tag="LR110_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL4821884.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22178..23050
                     /locus_tag="LR110_000885"
     CDS             22178..23050
                     /locus_tag="LR110_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL4821885.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23392..24264
                     /locus_tag="LR110_000886"
     CDS             23392..24264
                     /locus_tag="LR110_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL4821886.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24261..26219
                     /locus_tag="LR110_000887"
     CDS             24261..26219
                     /locus_tag="LR110_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4821887.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26551..27402)
                     /locus_tag="LR110_000888"
     CDS             complement(26551..27402)
                     /locus_tag="LR110_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL4821888.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27525..27992
                     /locus_tag="LR110_000889"
     CDS             27525..27992
                     /locus_tag="LR110_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBL4821889.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28255..28737)
                     /locus_tag="LR110_000890"
     CDS             complement(28255..28737)
                     /locus_tag="LR110_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL4821890.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28837..29700)
                     /locus_tag="LR110_000891"
     CDS             complement(28837..29700)
                     /locus_tag="LR110_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL4821891.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         8585 a         6371 c         5453 g         9692 t
ORIGIN      
        1 taagattttt ctaaattatt cctcattata aaatttcaag tgttttttta ttttgagttt
       61 tactctcccg taagcgctca ttacttgctt gtgatccatt gctaaacatt cagccatgtc
      121 tacaaaatca acgccttgca tataataatg aaaaatttta gcttctattt ttgaaaactg
      181 gatttcgcaa ttttcgattg cttcttttaa taaaagatgc tgaattgcta aaggttcttg
      241 caatgtgatc tctgctaaaa agtctggtcc ttctgtctct attttttggt cgattgagac
      301 ttcatctttt tgatcacgac gcttcagcgc gttctgcttc ctcaacagag aatagatttg
      361 attgcttata acgcttttat aataacttcc aaagctaaca cctttgcttt tttcgtaagt
      421 tctcaatgcc tgatataaag cgatccttgc ttcttgttcc caatcctcag aaacataatt
      481 tcttaaataa taggtgtttt tcaaacgtag taaaagtgga aaatattttt gatacagggc
      541 agcaaatgct tcttcgtcac cattacgtgc agcgcgcaat aactcctcag acataaaata
      601 attcctccat aaattcgttt atggaggaat cttatcatca atgattcatc gaactgatta
      661 gaacgatatg ttatttattt tttctcatta aatcgtccaa tttttgggac aattttttta
      721 actgctcttc attccatgga gaattacgtc ggaaatcaat aaaatgcata tctttatttt
      781 cttgggctat tgttttttcc gtttttttta tcgtcttata taattcataa gcagaagttc
      841 gtaatgctcc ttgggaaaag atcatccact gttctgctaa atcgcttgtc gccactgtca
      901 cttgtgtcaa acggtcattc agctgaccag cgattctctc gatataactg tcagccgttt
      961 cgtcttcttt cgtaaaaaca acatccaact ggtatttctt ataattttgt tggatgcccg
     1021 gaactagttg ggcatcgaag acaacgatga cttccattcc ttcatatttc gcataatttg
     1081 ataaacgatg taaaagagct tctcttgcat ccgccatttt gtcctgcttt ttaagctgga
     1141 caagttccgg ccacgaacca atcatgttgt agccgtctac aatcaacagc tgttttttca
     1201 tggtctactc cccctgacgt tgggaaaaag cctgatacat aagcaaacca gcagcaacac
     1261 tggcatttaa gctctggacg tgcccggtca tcgggatcgt cagcaattca tccatttctt
     1321 ttttcaatcc tggactcatt cctttgcctt cattaccaat gactaaagca attgatcctt
     1381 tggcattcca tttttggaag tttgtgccgg tcatatctgt gccaaatacc caaaatcctg
     1441 tttctttgag ctgttgcaca gcttgtacca aattcgtcac gcgtgcaaca gggacatgtt
     1501 cgactgcccc agtggaagtc tttgttacga taggtgtgat tccaactgct cggtgttttg
     1561 gtatgatcac accatcgacg ccacttgcgt ctgctgtccg taaaatcgac ccaaaattgt
     1621 gtggatcttc gatgctatcc aaaatcaaaa agaaggggtc ttcttttttc tcttttgttc
     1681 gttcaatcag ttggtcaagg gataaatatt cataaggtgt aatcgcaaga acgactcctt
     1741 gatgcacgcc gtgatcgctc aaagtttcta atttttgttt aggaacccat ttgactggca
     1801 cagcttgttc acgtgcgatt tctttcagtt cttctacttt ttctcctcgt gaatcttctt
     1861 gaagaaagag tttattccct cgcccttgtt gtagcgcttc tactgcggca tggtgcccaa
     1921 agacgaaatt atcttctaat gcttcttcat tttgatcaga aactgactga tttcgtccag
     1981 tttgcttttt atttgaatat ctattttgtt ttttgtcttc aaatccacgt ttcgattgat
     2041 gtttgtttgt tctttcttta cccatttttt tcaccaactt tctgaataca ccagcggatc
     2101 aattcttcca tccgctcggt ttgttttgtc aaatgcagat agcccatgag cgcttcgaac
     2161 cctgttgcga cccgatacgt cgtaacatca gcattttttg cgctcgtatg gcttttagca
     2221 tttcttcctc tgcgataata aagttcttct tcttctgtca aaagatgttc ttccagcatt
     2281 tgctgaatca aaaaggcttg tgcattcgca gaaacataat tcgttgccgc acgatgcagc
     2341 ttattgggtt ttgtttctcc ttgatcaatc aggtggtcac gaacatagat ttcgtaaatc
     2401 gcatctccaa cataagccaa ggctaaaccg ttcaattgtg tgtaatctct catgcttctc
     2461 ttctccatcg ggttccttgc ggtgtatctt ctaataagat ccctttttct ttcaacaaat
     2521 cacgaatttc gtcgcttcgg acaaaatcac gattgcttct tgcttgattt cgttcttcga
     2581 tcaattgttc aatctcttca tctaataact cttctgctgt aaataaaata ccgaagatcg
     2641 aaagccattg cgagaataaa gcttctcctt gtgccaagac agcggcagaa actttcggct
     2701 ggtctagata agtgttcagc cacttagcaa gctcatagac cactgtgatc ccatttgctg
     2761 cattaaaatc atcgtccatt tcttcgatga atcgctgttc taaattttct aacaaagcga
     2821 ttttatccgc atcatctgcc aaattctctg aggcagtttc ttggcggaat ttcaaattgt
     2881 caaacgtatt cttcagtcgt tgtaaattgg tggctgcttc tttcatcgtc gtttcactgt
     2941 agcggatcgg acgacggtat tgtgtggtcg ccatgaagaa acgcaagact tgtggatcaa
     3001 cgtttttgat catttcatgt acagtgatga aattccctag agatttactc attttttcat
     3061 cgtcttcgcc aatcgttaca tagccattgt gcatccagta attagcgaat tttttccctg
     3121 ttttcgcttc actttgtgcg atttcatttt catgatgagg aaattctaaa tcttgcccgc
     3181 caccatggat atcgatcgtt tctcctaaat gttttgttgc cataacggaa cattcaatat
     3241 gccaaccggg acgtcctttg ccccatggtg aatcccaaga aatctcgcca ggttttgctt
     3301 gtttccataa agcgaaatct aatgggtctt ctttttgctg ctgttcttcc cctgtacgct
     3361 gacttgctcc gacttccagt tcatcgatgg attggtcact taatttccca taattttcaa
     3421 atttacgggt acggtaatac acatcacctg aagattcata agcatagcct ttttcaacca
     3481 acacttgaat aaatgctaaa atatctggca tatgatccat gactcttgga tgcagtgtag
     3541 caggttcgac attcaacgct ttcgtatctt cttcaaatgc tcggatgaaa cgatcagcaa
     3601 cttctggtgc tgtaatacct aattcgtttg ctgtacggat gattttatcg tctacatccg
     3661 tgaaattcga tacgaaatcg actttatagc ctcgatattc aaaatagcgt cgaattgtat
     3721 cgaaagcaat ggtgctacga gcatttccga tatggatata attatacaca gtcggtccgc
     3781 agacatacat accgacatgt cccttttgta tagggatgaa ttcttctttt tttctggtca
     3841 atgtattgta gatttttatc attctgatct cctcactttt tctcctttga ttcgaacgac
     3901 ttttgctggt actcctaccg ctgtagcgtc gtctggaaca tctttcaaca caacggcacc
     3961 tgcaccgatt ttggcgtttt ttccgatcgt taccggtcct aatatctgcg cattcgcaga
     4021 gagcatggct ccttgtttaa cagttgggtg tcttttaccc gtttcttttc ctgtgccgcc
     4081 taatgttacg ccatggaata aaatcacatc gtcctcgatt tctgcggttt cgccaatgac
     4141 gattcccatt ccatggtcga tgaacacgcc agttccaatc gttgctcctg gatggatctc
     4201 aacaccagtt aggaaacgcc agaattgagc attgatcttc gccaataaaa agagccggtg
     4261 ctgatataaa aaatgagaga aacgatgcca aaacaaggca tgaaaaccag ggtatgtcaa
     4321 aacgatctca gctgtcgaac gtgcagccgg atcgtttctc tttgctgcat caatcgctcg
     4381 ggtaaaccaa ctcatgactt tccactcctt tcgtaagaat tttatgtagg aaatcatctt
     4441 cttataaaga aaagaagcgc cttttaaata agtttcctta tcaaaagacg ctttccgcgt
     4501 ggttccactt ttcttcataa tcaatgatta tctctgacag ctaacgcctg ttatacgttc
     4561 ttggctactt tgtttcctat aaaacagttt ttcctgtttt ctgtgtttcg ccaagaccac
     4621 tcccagaggc atttcggcaa ccattcattg ataccttgca ccacccggta tctctcttgt
     4681 aatgatcagc tgcttacttt tttctgttca tcgtgtttag ttcagtactt gatttaaatg
     4741 tgctaatgat ttttcacgac caagcaattc gatcgtttca ggcaattctg gaccatgcat
     4801 ttgtcctgat acagctacac ggattggcat gaaaagattt tttcctttta cgccagtttc
     4861 tttttgaaca gctttgatgg cagctttgac agttggagca tctactactt ccatttgttc
     4921 tagctgtgct ttgaatgcgt ggagaacagt aggaactgtt tcaccagcaa gtacttcttt
     4981 tgctgcttca ttcaatacag gatgttcatt gaaaaatagc gtagacagat caacgatttc
     5041 tgcagcatag ctcatttgag gttgatacag gctaacgatc ttcttcaccc attcgattgt
     5101 cttttcatct gggttttctg ctacacgtcc atcagcgatt agataaggca gacacatgtc
     5161 agtaaatgca tctagatcca ttgctttgat gtaatggttg tttacccatt gcagtttttt
     5221 cgcatcgaaa gctgcgggtg atttacttag gcgttttgga tcgaacattt tgatcaattc
     5281 ttggtgttcg aagatttcgt cttcgcccac aggtgaccag cccaataatg caataaagtt
     5341 gaacattgca tctggtagat agcctaattc gcgatattgt tcgataaatt gcaagattga
     5401 ttcgtcacgt ttgcttagtt ttttacctgt ttcactgttg atgatcaatg tcatatgtcc
     5461 gaaggttggt ggtgtccagc caaatgcttc atagatcatc agttgttttg gtgtattggc
     5521 aatatggtca tctccacgta aaacgtgagt gattttcatc aaatgatcat cgaccgcaac
     5581 agcaaaatta taagttggca taccatcgcg tttttggatg acaaagtcgc caccgatatt
     5641 atcggattcg aacgaaatag cgcctttcac catatctgta aatgcgtatt ctgtattgcg
     5701 aggcacacgg aaacgtacaa cagattctag accttgtgct tctttttctg cttgttcttc
     5761 aggtgtcaag ttcgcacatg ttcctgcgta atgaggcatt tctcctcttg cgcgttgtgc
     5821 ttcccgttct gcttctaatt cttcttctgt gcaatagcat ttgtatgctc gattgctagc
     5881 aagcagctga tcaatcagcg gttgatagat ttctttacgt tcagattgac ggtaagggcc
     5941 gtattctcca ggattttcag gagattcatc ccaattcatt cccaaccaag caaggttttc
     6001 caactggctt ttttcgccat cttcgatatt acgtttttgg tcagtatcct caatacgaat
     6061 aataaagtct ccatcattgt ggcgggcaaa caaataattg aataatgcag ttcttgcatt
     6121 tccaatatgc aggtgcccag ttggacttgg tgcatagcgt acgcgtactt tcgtcatttt
     6181 cttcctcttc tttcaaacta gactttgact aacagcaaaa attccgtcag ttacggcttc
     6241 tcaaatatca tttatttaaa gccactcagc acaaatttta ccttttctaa ggtaaatagt
     6301 aaagccaact ggttgaattt ccttaccagt tggctgaatt tcttttttat tttgattgtt
     6361 tagtcttttt ctctgtctct tttttattgt cgattcctcg tcctgcatgt gcaggtttag
     6421 caaagatcat gcgtccagca gctgtttgaa gggcacttgt tacaacgact tcgatacgtt
     6481 cattcatgta atgctgtcca tcttctacga caaccatcgt gccgtcatca aggtaggcta
     6541 ctccttgttg gcgttctgtc ccggctttta ctactagaac attcatcgtt tcaccaggga
     6601 tgacgactgg ttttacggca tttgccaacg cattgatatt aagaacaggt acattttgga
     6661 attcagagac tttattcaag ttatagtcat tggttacaat gacaccatct aaaagcttag
     6721 ccaatttgat caatttgctg tctacttcac tgatatcttc aaagtctcca tcgtacattt
     6781 cgacagatat accctcttct ttttgcaagg catttaggat atctaatccg cgacgaccac
     6841 gcacacgttt caaactatcc cctgagtccg cgatgtattg tagttcgtaa agaacaaaat
     6901 tagggatcat caaggttcct tctaagaatc cagtctttgt aatatcataa atccgaccgt
     6961 caatgatcac gcttgtatcc aagattttat atttatggaa attttctcct gattttcgtt
     7021 ctaacagttg actctcaact tgactagctg tttcttgctc gatttttttg cttcgtgaac
     7081 caaaaacttt cttccattct tcgattcgtg tcgttcccat tctcaatccc agatagccta
     7141 agatgatcat cagtaaaatc ggcaatacac tattgacgaa tggaatttcc atattgtaaa
     7201 gaggggtcga gatcacaaca cctaaagtca atccgatgat cgcaccgatc gcaccgaaga
     7261 gaagataagt caagctgacc tcacttagtt tttgttctac ttgtttgata atagctgaga
     7321 tatgtttggc caacagcaaa gataataaaa agaaaataag tgcgcctaac aggctgttgg
     7381 taaatgtatt attcagccat ccgtttgact gttgaccgat tgcctgccaa gctgagggta
     7441 aaaatgaaat acccaagctt gcgccgacaa tgatcatcag caaagtaatg acacgttttt
     7501 gcatcgtttt tcctccgttt cgacaatcat ttattagtta gcggaacaca cgcttcaacg
     7561 tttcagccaa agttgctact ccaactatct cgattccttt tggcgcttcc cagttaccta
     7621 agttgttttt aggtaggtat acttttgtga agccgagttt ttggacttct ttgactcgtt
     7681 gttcaatatg actgacacgt ctgatttctc cagtcagtcc aatttcaccg atgaaacatt
     7741 ccgttggctg tgttcctttt tctttatagc ttgaagcaat actgacagcg atagctaaat
     7801 caatcgctgg ttcatttaat tttaccccac ctgcagcttt taagtaagca tcttgattct
     7861 gaagaagcaa cccagctctt ttttccagca ctgccatgat cagagatacc cgattgaaat
     7921 caagacctgt tgttgttcgt ttggcatttc cgaacatagt aggtgtgact aatgcttgga
     7981 tctctaccaa aattggacgt gtcccttcca ttgccacaac gatagcagag ccagttgcac
     8041 cgtctaggcg ttcttctaag aaaatctgtg aaggattagc gacttcttcc aatccatgct
     8101 catgcatttc aaaaatccca atctcattcg ttgagccaaa tcggtttttt actgctcgca
     8161 aaatccgaaa actatgatgt tgttcacctt caaaatataa caccgtatcc accatatgtt
     8221 ctaacattct tgggccggcg atcgagcctt ctttggtaac atgaccgacg ataaaaatgg
     8281 ctatcccatt tgttttagcg gttttgagta gttctgcagt ggtttctctt acttggctga
     8341 cacttcctgc cacacttgtg atgtctggct gcgtcatcgt ttggatggaa tcaatgataa
     8401 cataatcagg ggagatatgt tcgatggcac gactgatttc gttcatatct gtttcagcgt
     8461 atagataaaa ttctgtgttg atacttccga ggcgttccgc gcgtaatttt atctgctctg
     8521 cgctttcttc gccggataca tataagactt ttccgcctat ggctgctaat tgctgagaaa
     8581 cttgtaacag caaagtggat ttcccgatac ctggatcgcc accaatcaag accatagacc
     8641 ctggcaccac acctccgcct aacacacggt tcagttcttc taattttgtt ttgatccgag
     8701 gttctttttt agggacgacg tctgcgattt ttgttggttt tgctttttct cctgtcaaac
     8761 ttgtccgagt tctgcggtcc gttgtatctt gttcgatttc ttcgaccatc gtattccatt
     8821 ttccgcaatt tgggcagcgt cccaagaact taggcgacac atacccgcaa ttttggcaaa
     8881 caaattgtac ttttgatttt tttgccattg taccgcctcc ttttatccta atcatgagac
     8941 attaaatcaa gcttaagtat ctttaacgtt ttatttactg gaagaaccaa atccgccttg
     9001 acgttgagca gtcgtttgat cttgatctgc ttttaaaaac ggcaaaaaga tcccttggcc
     9061 gatacgctcg ccttttttga tcactacatc cttgaaacca aagttggtga attggaacat
     9121 gatatggcct tcatttgcct cattattgta ataatcactg tcaatcacac ctactccgtt
     9181 tgtcaacact aaaaaccgct tcaacggatt gcttgaacgg ttagcaagct gcaaaaattc
     9241 atcttctccc atgtaagctt tgattccagt aggcacgagg gttggtttca attgtttttc
     9301 attttctgat acatcttttt tcaatccatc tgcgactgtt tccaacccct ttttccagcc
     9361 tgctttccag atacttggaa tcacgacctc ttctgctgct tcaaaatcat agccagcagc
     9421 atgatgtgta gcgcgctgcg gtagattgat attttgatct ttatatttag aaataacttc
     9481 aaatcctctt tttctcaccg gttttcctcc ctaaataaac tactcctatt ctaacatatc
     9541 cgaggctgta aaagactttc tttacaaatt cttcaagaaa ttctgctatc ttaaagtaaa
     9601 ccattaattc gttctttttc caaacgactg caagttgtac aaaaaaacta ttttttacat
     9661 gactttactc gtcaaaacaa gtagaataga acgaaattaa tgagtggata tttgattgag
     9721 gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt attgtcaaag taggaacaag
     9781 tacattgatt tatcccaatg gaaatatcaa tcttgcggca atcgatcagt tagcttttgt
     9841 cctttctgat cttagaaatc ggggaaagga agtgatctta gtctcatcag gagctgtagg
     9901 cgttggtatg cataaattac atttgtcaga acgtccgact ttgattcctg aacagcaagc
     9961 tgcggcggct gttggtcaag ccgaactgat gaatatctat aatcaaagat tcagtgtata
    10021 taatcaacag acggcacaga tcctcgtgac aagagacgtg gtagattatc ctgaaagtcg
    10081 taaaaatgtg aggaatacat tggaacagct tttgattatg gacacgatcc ctatcgtgaa
    10141 cgaaaacgat actgtagcag tggatgaatt agatcatctg acaaaatttg gcgataatga
    10201 tcagctgtca gccatcatgg caaaacttgt cgcggcagac cttttgatcg tcctttctga
    10261 tatcgacggg ttttattcag ataatccgac tacaaatccc gaggctcaga tgtatcgcta
    10321 tatcgataaa atcgatgaaa agattctggc acaagcaggt ggtgctggta gcacatttgg
    10381 aacaggcggt atgcagagta aattaaaagc cgcttctagg atatttgaag caaacagcgc
    10441 aatgatttta gcaaatggaa aaaatccggc aattattttt gatattcttg caggaaaaga
    10501 agtaggtact ttatttaagg aggacttgaa atgactgact tgatgcaatt aggacaacag
    10561 gcaaaagata gcgcgcaaca actagcactt atgcctacaa accgcaaaaa tgagctactt
    10621 gttcaaatgg cacaaaccat caaaaagaat caacaagcga tcattgaagc aaatcataaa
    10681 gacttagaaa aagcagtcga aaacaatatc agtgaaacga tgcaagaccg cttgcagctg
    10741 acacctgagc gaatcaatgc catggctaca gagatcgaaa aaattgcttc gttagacgat
    10801 cctattggaa aagtagatga gatgtggacg aacaccgacg gattgagaat cggcaaaaag
    10861 cgcgtgccac taggcgtgat cggcatcatt tacgaatctc gtccaaatgt cacgacagat
    10921 gcagctagtc tagcattcaa atcagggaat gccgttatcc ttcgcggtgg caaagaagca
    10981 ttcttctcga atcaattatt agtccaatta ttgcagcaag tactggtcag tgctggcgaa
    11041 tctccttatg ccatccagtt tgtcgatgat acttctcatg aaactgcagg acagttgatg
    11101 cgattgacag aatacttaga tgtgctgatt cctcgaggcg gagcaaaatt aatccaacga
    11161 gtcaaagaac aggcaacggt acctatcatt gaaacaggta caggaaataa tcacgtatat
    11221 atcgataaag aagcacagtt atctatggca gtgaacatca tcgtcaatgc aaaagcttcc
    11281 cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc attctgagat cgcacctttt
    11341 ttcttgccag ctattgaaaa agaactggta gaacacgggg tctctttacg tgcggatgct
    11401 cgtgcgttag aatatctaga gacggctgct ttagcagaag acacagattg ggatactgaa
    11461 tatctagatt atatccttgc tgtcaaagtg gtcgattctt tggatgaagc aattgcacat
    11521 atcaatcggc ataatactaa acattcagaa acgatcgtga cagatagtta tgcggctagt
    11581 caacgtttct taaatgaagt ggacgcggcg gctgtctatg tcaatgcctc cactcgtttt
    11641 acagacggat cggtatttgg ttttggtgca gagattggta tctctactca aaagttgcat
    11701 gcccgtggtc caatggggtt aaatgaatta acttccacaa aatatatcat ttatggcgat
    11761 ggacaaatcc gcgaataatg tttcgtgtga aacattattc ccgccttttg ccttttttct
    11821 gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg aaattttaat gatttttttt
    11881 atttacctta tttctctgaa tatcgctgct tttctgggag tcggtatttt atcccttttc
    11941 tttcaattca aaaaacgaag catagggagt caacgggaaa aatggtcgca gtattttgac
    12001 aaaatcgggc aaaaagggct ggttacacgc ttgcacatta gttacatggt tgcattatgc
    12061 ttattagcga caatcaatta ctattctttt ttcgatcatt caatcgctta tacgattacg
    12121 ctactgattg ctgggatttt tcacttatct tataaatatc agctgaataa aaaccacttg
    12181 aatcggactt tccgctaaaa aatatccgta cgataagtag agtgccttga agatcggcaa
    12241 aaaactgccg gactttttag aggctgtgac aaagattttg tcacagcctc tttttccata
    12301 taaacggcgt tcgcctacca gctgcctggt aaatctgttt cctcttcgtt aagaagcgaa
    12361 aatccgtcaa atgtttcgcc atcataataa cgaagcagct catccatgga caagttcatc
    12421 gttttttctt tttcttcttc cgctgattca gccagtagct gttcccgata cacctgatac
    12481 ataccagcta ttttttgctg catcgctttt ctagtttcac cttccgcatt gattattggc
    12541 aattctttga tatagccacg ataaatcaac cgatcatctg aaaattcaat gggtaattca
    12601 acgagtgtca acccgacttc ctgaaaatat tcttccagca tctattatcc ccccattctt
    12661 tttcttaaat gaattttagc ttacctactg aaaattttcc agtttcatta ttttgagaaa
    12721 aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt ccgaataaat ggtgttcttc
    12781 tgccaactcc tcaatcataa gtcacaatat tcaacaaaca tgagaaactg ttttttgaaa
    12841 ttgttccttg tgattcagag ttaaacgaca gtctcacaac ctcttggtaa acggcgttca
    12901 atcagctgac cctcggtatt agctcaaaaa acggtaaaat atgagaagct atttttccgt
    12961 tttgtttctc taatacttag gataaaacgc tgattttaca acctctgtta aacggtgttc
    13021 aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac tatttttccg
    13081 tttgtttctc taatactcag gacaaaacgc tgattttaca acctcctgat aaacggtgtt
    13141 caaaagcttg catctgcggt aataagccca accaaacgaa aaatatgaga agctattttt
    13201 gtttggctgt tcttatgact tgatgcagga cagctttttc acaacctctg atttcttatt
    13261 cttcttcatt tgtagcaaaa taattacggt gataaatgat acaacataca ctgataagaa
    13321 caatggcacc tgcaaaataa aacgcatagc tttcaccaat atgttcacca atcacacctg
    13381 caataaatgg tgctaaagct ccaccgaaaa agcggacaaa actatagctg gacgaagcaa
    13441 cgtttcgttg cagacctggt atttccatag caatcgtcgt caacagtgta ttgataattc
    13501 cttggaaaaa tcctgctaac acaatgctga tcggtacaag gactggcata gaagttccag
    13561 ctcctaataa cagcaaacaa ataatgaata agacaaatgc tgctaaaatt gtccgataag
    13621 tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga agcaatagcc agaagcacac
    13681 cccagccaaa aaatacaaaa ccgacttcta attcagaata gccaatcaat aaaaatggcg
    13741 catacgcaag taaagtaaag aatccgaaat tatataacaa tgcgattagt ccaactgaac
    13801 gaagtttatg attgcttaac gctttgacac cttcccaaag gcctactttt ctctttggtt
    13861 tctcagtttc aggtaaaagt accgctaggg ctataaaacc gcaaaacatc aaaaatgcta
    13921 ccccaaaaaa tggataacgc catgaaccac tgcccaagac cccaccgact agagggccaa
    13981 cagccattcc gcagccaagt gctgcttcat acatcatgat tgctttttct gtgttaccaa
    14041 ctaaaatact gacgatcgtt gctaaagcag tagagacaaa taaagcattc ccgacacccc
    14101 agccggcacg taacccaatc agcattccga tattcccaga taatccgcca agaccagcga
    14161 atacaacgat gataaacaat cctgtcatca atgttttttt caaaccgatc cgactagata
    14221 agaaacctgt aaatagcatc accacaccag tgactaacat gtaactagta aataacaggg
    14281 tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt ttttaagatt gggtctacta
    14341 gacccactcc cataaaagcg atcatacagg tagctgctac aactagcgct gaacttttcc
    14401 cctttgtttc ttcatttgtt tgttcttgta tatctatttg attctccaat atttccatcc
    14461 ctttctctct attagcatca tacatgattt attttacatg tcaatatgca catgtaaaac
    14521 gtggaaactt tactgtgata taataaagaa aataagacga gaggtaggat ttttatgaat
    14581 actttagcct atatattatt atgcgtgctt ttatccaaac cacgttctgg gtatgaattg
    14641 aagcagctga tcacaatttt ttgggaagcc catcacagtc agatctatac aacgcttgcc
    14701 aagttagacc agcagggtta tgtgaccgtt ttggacaaag acgagcactc tcaaaaaaag
    14761 atctaccact tgactgatga aggacgtgct cttgtagagg aatggatcaa agaggaaact
    14821 cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct atgctttttc gacgctcgat
    14881 aaaacaacag ctactggact gctattcact agagaacaac agctgaacaa aatcatgcaa
    14941 aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga aacaagaatt tggacgttat
    15001 gttgtgatcc agcgcagaat tatgctttgt cagcaggaac tccaatggtg tcagcaagta
    15061 agagaacaaa tgcagcagtt ttttgagtaa gggataaaca gaacctttct agaaatatct
    15121 attgaagagc aaattatctt caataaaaga gcaatacagt tggaaaagaa aaagcgatcc
    15181 gaacgacagg ccaaaaccta tcgttcggat cactattttt ctagaactgc attcaagaga
    15241 ctatgacaaa aagcatgcca aggccttttt tcaaataaca gtgttccatc attcattact
    15301 ttgaattgct tttagcacac ttcttgaaag cgggccaacg atgatcaact gcaacggtaa
    15361 agcggcaatc acattcattt tccaagcatt catataagca gagaataatt gatcaggtat
    15421 tccttgttcc atgatcacac cgaataaaga catacatgtc accattccta aaaccatcaa
    15481 gcatgaaata gttaagatca tttggacctt gctttctgga tttactggta gtttaaaggc
    15541 aatttttttc gccagcacac cgatgatgaa gacatccaaa atgaaagcca ccacgaatcc
    15601 aggcactaga ccaatcaaca aacttgataa atgaagatct tgatgcagcc ataaattata
    15661 cgcgctcatc cccaagacca tcaaaaaaca catcattgtt gtaaaaatta ttccttcttt
    15721 tttatttgta ggcaatctgt accactcctt taaatttgtc acgaaaatca tcgtagcaca
    15781 tttaaaaatt tctcgtaagt acttttttta ttttctgccc ttttatttca taaacttcat
    15841 cagccgtacg ctcgacaaag taagaatcat gagaagtaaa caaaatcgtt cccggatata
    15901 tttgcattag cttctctaaa gcttcgatcg ttgccatatc aataaaatta gtcggttcat
    15961 ctaacagcaa cacattactt ggcttagtaa aaagcaaagc aatcgtcaga cgggtcgctt
    16021 ctcccccact taatgtacat aacggtttcg tcaaagtttc ctgagcaaac cctaagtgat
    16081 tcagcaaact acggacaagc gattctgaaa actctgtttg tctcatgaca aaacgaatga
    16141 tggattcttc agatgtcatt tgataaccct tttgctgata tacttgaaag cttactttcg
    16201 gagagagaag gattccttct ccttgtttgc ggatatgttc taataaggtt gtcttacctg
    16261 aaccattttc accgacaatc gcgatttttt tacctaaaga aaactgaaaa tcaccattta
    16321 ccagtaatgt tctcccactt ctttccaatt ggacattttg tcccatgatg ggatagcggc
    16381 tgtggagttc aagagatttt ggtactggaa aacggatttg tttcatctgc tgcggcttgg
    16441 ttgtttctcc tattttttgg agtcttctct ctaatgtttt cgcattcttt tggatggctt
    16501 tttgtacact gtctttttgt ttagaggaag acaaccgatc cagtctgatt tgttgctttt
    16561 tctttttact ggacactttt tccaactttt ccgcttgttt tttcttttct tggatcgatt
    16621 tttccaatcg ttttttctct ttcatgatcg actctgcttc tcgtagctga gtctttttct
    16681 ccaattcctt ttgagtgaga taggcactat aattccccga aaactctcgg atttctccgt
    16741 cttttacttc ccagattttc gatgctagct catctagaaa atagcgatca tgactaacaa
    16801 aaaccagcgt accataataa taacgaagct cttcaatcag tcgatcaata ctttttctat
    16861 ctagatgggt tgttggctcg tctagaagca tcccttgttc ataagaagaa agaagacgta
    16921 tcaactcgat ctttcgttct tcccctccgc tccatttccg ctctgaagtt tcttccagtc
    16981 ttaactggaa atgactggct aattccaaat ttaggtcctt tgcttcagcg agataggtta
    17041 attgaggcag gtagttgaag gtgatctctg tttggatttt cccatgatcg ggaaaaagtt
    17101 caccggctat tagtttgagc aaagtcgatt taccgacacc attttctcct atgatcccta
    17161 tacgatcttg ctgatagaca cgaagagaat caatagatag ttctagctgg ttgccaaagc
    17221 tgacttgtag atttgttata tttactgcta aattttccat atgttttaca ctcctatttc
    17281 atgtaaaaca acaattatcg atactaaaaa agagcagaaa aatctgtttg caaaaaaact
    17341 gcatcacatc ttttcctact ctgaacgaaa taactatgac taaatcacca aatctgtcat
    17401 agctttcacc tattctactg tcgttcgaaa aaatggacat actccatcca tgaaattgtt
    17461 gtttatacat gtcttttgaa tttatttagt tgttattcaa aagttcgaaa cgtaatttca
    17521 tcgatgcagt catttttccg acctcctttt ttattaggtt accacacacg attcaaaaaa
    17581 agcaatagct actgaatagt aactgaaaac cttttattcg aacatagtga tcttttctgg
    17641 tgctaaacga acagactcgt aaccttcttc taccgctttt cctattgata aaataataac
    17701 tggaacataa cggtctttat ccaaaccaaa tgcctctgcc aactgatctg cttcgaatcc
    17761 accaattgga tttgtttcat agccatgatc acgcgcaact aacatgaatt gcatagccgc
    17821 taagctagaa tcgactttga ctacatcgtt catttcttct cttgaaaaac tcttgtaata
    17881 aggaatgatc gcagctaatt gttgatctct gacttctttt ggcatcttcc ctgattcgta
    17941 agcttggttg tagatttctt cgccatattc gtaacattgc atatcgccaa aaatcatgac
    18001 cattgcagaa gaagtatcat tttgtctcgt attgaaacgg atcaaaggac gcaagatttc
    18061 ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt ttcatattga cagaagaagg
    18121 tgcagtcgtc gcttctttga tcatttccag catttcttcg tgtgggattt tgtatgtttc
    18181 atcatatacg cgaacggatc ttctaccagt cataattgtt gaaaaatcat ttttttgttc
    18241 cattattatt aacctctttc gtttctgttg ttatattgca aagttgggta ttcaatttat
    18301 ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa agccggggat agcacggaac
    18361 ctgtatcttt atggtccatt tcacaatgct tgattttctt ctgggctttt tctgttagcg
    18421 ctacatacac ttcccggttg ttctgaggat ttctctgacg cgaaacatag ccatttgctt
    18481 ccaatacctt cagatgacga gtaactgctc cttgatcaat ctgcaacttt tcttgcaaac
    18541 tgctttgtag caatggattt tcttctttca gtctcatcat gatctcgtat ctagtaagac
    18601 ttataccgat attttcttca aagatctgga cgattttttg atcggctaat ttgatttgat
    18661 ataaaaggtg actaatctct tctaaagaca taatttcctc ctttctaata attgactcgt
    18721 caacgattga cttgtcaata atagaataaa aaaagaaaaa agtcaaagaa tcagctgtgt
    18781 aataaaaaaa acgctctcaa tagaaaagta tagagaaaag aaattttttg tagaatgaac
    18841 ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat tattgattct tatcaaacta
    18901 tagaagatag ctcttctgca gtagataatt ttacaccgcc aaaaatttcc gaaatcgcct
    18961 tttgcaagaa tcccgtcgaa tattagaaat tggatcagta tgcttaaaca gtaataaaaa
    19021 agttatgaca agatgttgtc acaacctttt ttgagtaaac ggtgttcaaa aacttgcatc
    19081 tgcggtaata agttcaacca aacaaaaaac atgaaaatct gtttttgttt ggctgttctt
    19141 actacttgat gcagggcagc tttttcacta cctcttttta ataaccataa aatcctattc
    19201 cttcatatct ccagcccatc ggtgttagcc aatctctttc cttagcactc aaggtatagt
    19261 gatgggtacc agttttttca tttggattaa atagtcgata aattggcttg gaaccaccag
    19321 aataaaaact tagaccttca taacgccaac cgatttttgt taaagagttg atctcagatt
    19381 gtgccattgt gtaatgatgg tctccactgt aaggattgta taaacggtag acaggttgtc
    19441 cgctgtttgg tgcttgccaa gcaatccctt catattccca gcctacagtt ggcagataat
    19501 ttttttctcc ggcattcaat gtatataaat gttctcctgt atgacggtta tacagacgat
    19561 acatcggaga agttgaagtt ggtgctgtat catactgtgt cagattatag gtttcgatta
    19621 cattgttcaa tgtagaacca taattaggcg cagtggcata acgacctgtt agccaagccg
    19681 tagcatcttt gtaagagttc gtattgcttt tccatgcacc ggaatagtag taaacgcctg
    19741 gctgaaaaga agtcgtcttc aataccgtta cgttatcttg caatgattct ttgtacgaag
    19801 gatattttct aaaagcggca ttcaccgtaa tccattgccc attcacatat tccgaagtag
    19861 gcatcgtaac agattggcca ttatagcttc ctttaatccc aaatagattg taattagggg
    19921 cagaagccaa agtactgttc ccccagccac tttcaacgat ggcttgagca atcatgactg
    19981 atgcatataa gtcattggca cttgccaaac tttgtgcagt tgaggcaata ctatcaatga
    20041 acgcttgttg agaattagca gatgagcgag cataaaaagc atagccagta tagttttcga
    20101 attcagacaa atctgcttga tgggtatgtg tcaatgtttg cccatctgtc aattggttcg
    20161 tgatttcttc tgcattctct ggattgttct gagcttctgc tgatgtactg tcttcggttt
    20221 gtgtttgttc tgttgtttca gttgttggtt cttcggttga aattgtattt tctgagacag
    20281 tagctgactg caaagtactt tctgtatcgt tttcaattcc tgtttctgaa gaactcgatg
    20341 tctgagttgc gacagtttct gtcgattctt cagctagcac tgttgtgttg gcaactgcta
    20401 gatttcctaa aagaataacg ctaaataata aattctttgc tttcatcttt tttctccttt
    20461 gaattatgaa acttttccta aataacagtg taactaaaaa agtaaaagca agtcaatgtc
    20521 catatttaaa taacatttgt ttctttttaa taaatttgct ttttctgcat aaaaagacgt
    20581 aaatgaagaa tattcttctc cattcacgcc ttaaattgat ggtcgtttga ataactattt
    20641 aacaagtaat tctctctgga tattatccgt tatggattat acttcctgtg tcgtcggacg
    20701 gataatcatg ttgctgacag acatccggtc tggcgtttct atcgcaaatg ctacagcaga
    20761 agctatatct tcagatgtta atccccattc tttttgcgct tcatgaagtt caagagcagc
    20821 ctttttgtcg ctgatggttt gatataattc agtttgaaca gctcctggag agatgatcgt
    20881 agatttgatg ttattttgcc gttgctcttg acgtaatcct tccatgattg cacggacagc
    20941 aaattttgtt ccgcaataaa cggcggaatc aggataaaca acatgtcccg ccacagaatc
    21001 agttgaaatg atatgcccag atttttgttt ttccatgatt ggaagtacgg cggaaattcc
    21061 atttaacaca cccatgatgt tgatatcaag catattttgc cattcatcac ggtgaccttc
    21121 aactaatgga gcggttggca tgattcctgc attattgtac aatacatcga ttcgaccgaa
    21181 cttttccatc gctaaatcga taactgcttg gacttgtgca aaatctctta catctgctgt
    21241 ttgaatatat agctcagctt ctggtaaaga ttcttttatt gcttttaggc gatcttctcg
    21301 gcgtgcagct ataactagtt tagccccttt ttctgctaat agcttcgttg ttgcttcacc
    21361 gattccgctt gaagcgccca tgatgacaat cactttttct gataaatcat tcattctatt
    21421 ctcctcctgt tttgaaaaat ctcttgctta tactcactat ctcttatttc atcttactgt
    21481 tcaaataatc cgttcgacag gtttgacgat tttggcaggg attcctgcaa cgatggtatt
    21541 gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca atggcattct cgccaatcgt
    21601 tacgcctggc agaatagttg ctcctgctcc gatccaagca tttttcttga tgtggattgg
    21661 ctttactgac aagcctcttc gttcttttgg atcggttaga tggttcaccg tgatgaggtt
    21721 gacacgagga ccaatcaata ctgaatcttc taaacagatg ccacctaagt caacgaacat
    21781 cacttgacga ttgataaaga catttttccc tatactaata tgtttgccga aatctgtata
    21841 aaaaggcagc agaacagtca ccgtatcatc tactttgtca ctaatgatat tttctagccg
    21901 tttgcggata tctgctggtt catggtaccc agtattcagc gctgctaaag gctcttcgtt
    21961 ttccgcttgg atctgatgga tcacttcaaa gagcggatcc gttactcgaa tctctttatt
    22021 gacgatttgt ttttcgatga ggcttgtcat tttgctccct ccttttatcc ttactttata
    22081 acggacggct tatgatgtct aatacttata aaacatactt aactattcct tttaactata
    22141 cttagtggta tgctttttta aaaacaggag ggaaaaaatg gatattcgta tactgacgta
    22201 ttttttagca gtagctagag aaaaaaatat cagccgagca gcaaaatctc tgcatatcac
    22261 gcaacctact ctctcaaaac aactgaaaga tttagaagaa gaacttggtg tgacactttt
    22321 cactcgaggc tcccgagaga tacagttgac ggattctggt cgatatcttt attcaaaagg
    22381 aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat ttaacaaatg acgcaatcat
    22441 ctctggagaa atctacattg gcggaggcga aactaaagca atctctttca tttctgatag
    22501 ccttcattcg ttgatcaagg cgcatcctga tatccagttt catttatata gcgggaatgc
    22561 agatgatatc atagacaaac tggatagcgg attacttgat tttggactcg taatcgaacc
    22621 gacaaacaaa caaaaatacc agtcatttcg actgccagcc caagatcgtt ggggactttt
    22681 gatacatcgg aatcatcctt tagcaaagca aacagtcatc catccgcagg aactgaaaac
    22741 tgttcctttg atcatttccc ggcaaacgtc agtggacagt caactagctg aatggctagg
    22801 tttcagcata gatcagttat cgatcgtcgg aacatataac ttattgtaca atgcttcttt
    22861 gatggtcgat aacgggactt ttggtgcatt agctctagat gggatcatca atacttatgg
    22921 gacggaaaaa gtattcatcc cactcgatcc gccattacaa gcttcactga atctcatttg
    22981 gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt ttagatgtcc taacggagaa
    23041 atacagttaa gtagagtttt tttccttcgc tttcagcttt ttaacaacga ataagttaag
    23101 caaaagatcc aatgattaaa taacataagt ttcttaattt gttccttcct tttatttctg
    23161 ataattccag taaattacta taattataaa aaaagaattg acaaaacata actgtctata
    23221 taaaattgat tacgttaaca ataattcgtt tcatgtgact agatcatact aggcatgaaa
    23281 aatctctgag gaacatggtt tccctaggag gtttttcgtg tctatttttg tttggccgca
    23341 atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa agggtgcgcg catgaaaatc
    23401 aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg gtggacagga aaaagtagct
    23461 attggaaaag ggattggatt tgacagaaaa agaaatgatt tgatttttga tcgtgatgtc
    23521 gaacggatat ttgtcatgga accagaaggt caaatcaagt tgcaaaattt acttaatcag
    23581 attgatgaac gctttttatt tgcagcagaa acgatcattg atcatgcgga aactgttttg
    23641 atggaaaagt tgaatgaaca tgtattgatc gctttgaccg accacattgc tttttctgcg
    23701 gagaacatta ataacggtat cgtcatccgc aacaaacttt taagagaaat cgaagtgctt
    23761 tacagtgatg aattcagtat tgctcaatgg gcagtagagt atctgacaaa agagcttggg
    23821 attccatata cttatgacga agcaggatat atcgcaatcc atattcacag cggacgaagc
    23881 ggtcggaaca ataaccatcg cagtatccgt gaagtgacga ttgtatctga catcatccat
    23941 ttagtggaat cagaattgga aacagatatc cattcagaac cattttcact gaattattca
    24001 agactggtga atcatttacg actgcttcta caacgaaccc atgcgcaaca atatgctgtt
    24061 ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc cagaaagcta taagatttct
    24121 aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg cgatcacgaa agaagaactg
    24181 ggttacttgg ccatccatat cgagcgttta agaagcgcaa tcgtacaaac aaatgttaac
    24241 aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg aatgggacgt tcccttcaac
    24301 ttccagttgc ggtcttacct gctgctgctt tactagtagg gatcggaaac tggtgggcat
    24361 cttatagtaa cgacattgtc gctcattttt tacaagctgg cggtaacgca gttttaggtc
    24421 aattaccttt gctgtttgcc gttgggttag cactagggat gtcagtggac aaatcaggtg
    24481 ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt aaatgtttta aaaactgatt
    24541 cagtagctac tttattgaat atcaaaccag aatcagttga tccatctttt gctcagatca
    24601 gtaacgtatt tattggtatt attgccggat tgattgcggc agctctttat aatcgtttcc
    24661 acgaagcaaa attaccgatg gctttatctt tctttagcgg caagcgtcta gtgccaatct
    24721 tggctgcctt tgtcatgttg ctgatcagtg cagtgctact gctcgtatgg cctccggttt
    24781 actctgcttt agtttctttt gctaaattca ttttgagttt aggctggggt ggtgcaggtc
    24841 tttatggctt cttcaaccgt ttactgatcc ctacaggatt gcatcaagca atgaactccg
    24901 tgttctggtt tgacgtagca ggaatcaatg atattggtaa atttttggct tctgaaggaa
    24961 caaaaggcgt aacaggaatg tatcaagctg gatttttccc aatcatgatg ttcgggcttc
    25021 cagcaggagc atatgctatt tacagaaatg cacgtccaga aaacaaagca aaaacagctt
    25081 ccttgatgtt agcagcagct tttgcttcat tctttactgg tgtaacagaa ccacttgaat
    25141 tctcatttat gttcgttgct tggccattat atgtcttgca tgcaattttc actgggattt
    25201 ctttagcagt gagtgcattc ttccattgga cagcaggatt tgcctttagt gccggatttg
    25261 tcgattactt cctatctttg aaaaatccga ttgcgaatca gccattgatg ttgatcgtcc
    25321 aaggattagt aacagcagtg atttactact ttggctttaa ctttgctatc aaaaaattcc
    25381 atctgatgac tcctggccgc gaggaagcag atctttcaga tgacgatact gcaacgacta
    25441 attcagacaa taaatatgcg gcacaagcaa gcaaaatcta cgctgcatta ggcggagccg
    25501 acaatgtaac ttcgatcgat aactgtacta cacgccttcg tttgcaagtc aaagatacag
    25561 gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc tggtatgaag atcatcgacg
    25621 gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt cgttgcagat gagatggcaa
    25681 aactacatgg tggtgctgca acacgacctg cacaaacaaa caccgtggtt aaaacagaaa
    25741 catttgccgg tggaacgatc gaacaagata tttacgctat cgcaaatggg aaactgattc
    25801 caatcactga agtttcagat gatgtattcg cggaaaaaat gatgggtgac ggctatgctg
    25861 tattaccaga aaatggagaa atcttctctc caatcgctgg tacgatcaca aacattttcc
    25921 caacgaaaca tgctgtcgga atccaaacag atgctggtat cgaagtcttg cttcatatgg
    25981 gcatcaacac tgttgactta aaaggcgaac cattcacttt atatgtaaaa gaaggtcaaa
    26041 aagtcgcaag aggtcaattg attgctttag ttgatcttgc cgctattcaa tctgcaggca
    26101 aaaacacgga tatggtcgtt gtattcacaa atggcgacaa agtccaaagc ttagaaatcg
    26161 aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc tgtttccaac aaagcctgag
    26221 ccatcgtctt ccccagactt ttttatcgat aaacaaacag gagctgagac aaaaatcttg
    26281 tctcagctcc tttttccgaa taaaaacggc gttctcctgc caactccgcg gtaataagtc
    26341 cagccaaacg taacatatga agagctattt tttatttgac tcttcttatt acttgatgca
    26401 gaacagcttt tttacaacct ctgataaacg gtgttcaaaa accagtttat cggtatgaag
    26461 tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttca tactcaaaac
    26521 taatcggctt tttcacaacc tctttataca ttattcctga tccaatattt cttttaatgc
    26581 tgcttcaaga gaagttaatg gttttcccaa tactttttct aaatcatcag aggaaatagc
    26641 taactgatcc tttttaatat catgttggat cgctaaaaac atgtcagctg cttcttgagg
    26701 gaatccggct tcttttagat tttcgacaaa cgctgcatca cttgcttcaa taatttctaa
    26761 ctttttacct gtcgcacgct ctaatgcctt ggctaattct tcatagtcag ttagattgcc
    26821 ggataattct aagatttctg gagagtcact ttctactagt acttttgctg cagcttctgc
    26881 atattcacgt ttcaatgccc aacctgtttt tccatttcct gcagcataaa cgaattgacc
    26941 agtttttaaa gcctgcccaa tgataggtaa ttcattctct acataccaat tattccgtaa
    27001 aaatgtatgg gaaatacctg aatccttaat cattttttca gtgaattggt gatctggcgc
    27061 taatacactg atcgagtgat cggcatctgc aaaacttgta taagcgatat aagatacccc
    27121 agctgcttgt gctgccttca ctacattctt atgttcggct tgacgatttc ctggaacgcc
    27181 agagacaaaa agcaatcgat ctattccttt caacgcttct ttcattgagt ctaaatcatc
    27241 ataatcacca atacgtatat taaatccagc ttctttcagt gcttctcctt tttcttcact
    27301 acgtactagt acatagattt cattttgtgg aaccatcttt tttaagaatt ccagcgcata
    27361 accaccaaaa cctccagttg ctcctgttac taaataatgc atatttgcca ctccctattc
    27421 tttttatttg ttaagattta cagctgtaca tttataaatt acacctttat cataaagagt
    27481 gtggtaagat aagtcaagca aaaaaaatga aaggatgaca actgatgaag tattctctgc
    27541 aatttagtga tgcaatccat gtacttgcct atattgaaat gtttcaagat acccgatcac
    27601 tttctagtga gatgattgcc agtagtatcg aaacaaatgc agcaaatgta cgtaaaatca
    27661 tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac cggaaaaccg cagccttccc
    27721 ttgccaaaag tccagatcag atttctttac tagatgtcta tcgcagcatc gaaggtaata
    27781 cacagcttat ccaagtcgat ccaaaaacaa atccaaattg cgtcgttggt gcgaatatcc
    27841 aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa ggcagaagaa gaaatggcta
    27901 agatcacact tgatagtctg atacataaaa tagccgtttt ggaaaaagaa aaaagaccag
    27961 aaaacacagg cctcgtagag agatttttat aattctctct actgtgcctg catttttctg
    28021 atcggaatcg tctacataaa ttcttttatt ctccggggga caaaagcctt gtcatgcccc
    28081 tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat gacgtcttga taaacggtgt
    28141 tcaagtagct gaccctcggc attagttctg aaaaatgcaa aatatggaaa gctatttccc
    28201 atttttcctc tctaatgctc aggtcaaagc gctactttca cgacctctat attatcatgg
    28261 tttattgact accggtatcc agatttctcc catatatttc ccgttggtaa ctcccatttc
    28321 gactacagca tttggacccc caacataggc gtaatcttct acttctctta aaacatgccc
    28381 aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc gattctgcta aagcgattac
    28441 aacaagatac gttccttttg ggaatgggat gattcgggta gtgtttggaa catcctgact
    28501 cgtttgaaca ccgacatagt acatcatttt cccgttatag ggttcgttga ctgtaaagaa
    28561 ataatgattt tcagcagctt gtttcaatgc atctattgtt ccattaccga taactttttg
    28621 ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc gtattgctac tcttcaattc
    28681 cacaccaata cccgtcaccg taaaatctgg tttttcttcg atcctatact ttgccatttc
    28741 tcccaccctt tctattatat taagttcatc ttacataaaa ttaaagggaa atgacacgat
    28801 tacgctcttt tttctaggga tatttttatt ttttaactat ttttttgctt tcactgcaat
    28861 ccaaatctca ctgtaaacat tactgaaatc ctctccccat tttgtaaaag agatctcggg
    28921 agcttcgact aactcataat ctgaagaagg aagccattct gcatagattt tcccccaagt
    28981 atcttgtaaa gtttggggaa atggcccttt attaggaaaa atggcccaag tgtgttcttc
    29041 tatagcaagc atttctaaat cagcgtacgg attttctttt gtcgtagcaa agccaatcat
    29101 gtgcatcaaa ctgccctttt cttccaagcg ggctccatca aatccataag aaacgttgat
    29161 tacttgattt ggatataaat ctcctaactt atgcatctct tccctttgag tctccgtgat
    29221 cgtctgcgct aacttttgta tttcttgatt gactccttca aatttgacag gaactttttt
    29281 cgccacacct gccacataaa actgttcttt cttttcgata cgaaattcca tcgattttcc
    29341 tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat ttttgtatgc cgtatctgct
    29401 gatttctgaa ggcagatatc ctgaccaatc acgaaaagct cttgaaaaac cttcgactgt
    29461 ttcatatcca tatctaaacg ccgtttctgt cactcttgat ccatctagga gatcgcgatt
    29521 agccaaagcc aatctacggt ttttgatata gtccattagc gacatccctg caataaacgt
    29581 aaagatgcgt tttaattgat attcagagat tcctgtattt tcggctatta ttttatagga
    29641 aagtccttct gtcaaatgtt cttcgatgta atcgatcaat tggttcagat ctttaagcat
    29701 atttatcttc ctccttgctt gcctttttat catattatca aagaaagtca attacttgat
    29761 gaatcaggaa ccaatttagt cagatgtaga aaatgttgta tggatctaaa gaaaattctc
    29821 aagcagcgtt tcgtaacgga taattggtat tatctgccta tctatgtttt acctttagaa
    29881 aaataaattt tggggaagac acattctaag taaacaaagc ggtaaaaata tcttttattc
    29941 ccttagttaa tccgcttact acctactgta caaagcaaat tgttgttcaa actgctgatc
    30001 agaacgctgc ttggattttt ctttgcgata catcatacct cttttatcaa ttggaacaat
    30061 tatttatttc taacaaaatc acttcatttt tgtcagataa a
//
LOCUS       DAFFSV010000022        27699 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFFSV010000022 DAFFSV010000000
VERSION     DAFFSV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.022"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(209..1153)
                     /locus_tag="LR110_000892"
     CDS             complement(209..1153)
                     /locus_tag="LR110_000892"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL4821892.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1449..2393)
                     /locus_tag="LR110_000893"
     CDS             complement(1449..2393)
                     /locus_tag="LR110_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4821893.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2614..3828
                     /locus_tag="LR110_000894"
     CDS             2614..3828
                     /locus_tag="LR110_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4821894.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3850..5865
                     /locus_tag="LR110_000895"
     CDS             3850..5865
                     /locus_tag="LR110_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBL4821895.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5948..7321)
                     /locus_tag="LR110_000896"
     CDS             complement(5948..7321)
                     /locus_tag="LR110_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL4821896.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7347..7631)
                     /locus_tag="LR110_000897"
     CDS             complement(7347..7631)
                     /locus_tag="LR110_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4821897.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7633..9648)
                     /locus_tag="LR110_000898"
     CDS             complement(7633..9648)
                     /locus_tag="LR110_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL4821898.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9827..10132)
                     /locus_tag="LR110_000899"
     CDS             complement(9827..10132)
                     /locus_tag="LR110_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBL4821899.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10221..11252)
                     /locus_tag="LR110_000900"
     CDS             complement(10221..11252)
                     /locus_tag="LR110_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL4821900.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11459..12334
                     /locus_tag="LR110_000901"
     CDS             11459..12334
                     /locus_tag="LR110_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL4821901.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12486..13328)
                     /locus_tag="LR110_000902"
     CDS             complement(12486..13328)
                     /locus_tag="LR110_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL4821902.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13420..15297)
                     /locus_tag="LR110_000903"
     CDS             complement(13420..15297)
                     /locus_tag="LR110_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL4821903.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15294..16715)
                     /locus_tag="LR110_000904"
     CDS             complement(15294..16715)
                     /locus_tag="LR110_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL4821904.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16718..20107)
                     /locus_tag="LR110_000905"
     CDS             complement(16718..20107)
                     /locus_tag="LR110_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL4821905.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20364..21755
                     /locus_tag="LR110_000906"
     CDS             20364..21755
                     /locus_tag="LR110_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4821906.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21828..22475)
                     /locus_tag="LR110_000907"
     CDS             complement(21828..22475)
                     /locus_tag="LR110_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL4821907.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22483..23169)
                     /locus_tag="LR110_000908"
     CDS             complement(22483..23169)
                     /locus_tag="LR110_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4821908.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23175..24248)
                     /locus_tag="LR110_000909"
     CDS             complement(23175..24248)
                     /locus_tag="LR110_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4821909.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24470..25144
                     /locus_tag="LR110_000910"
     CDS             24470..25144
                     /locus_tag="LR110_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4821910.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25149..26489
                     /locus_tag="LR110_000911"
     CDS             25149..26489
                     /locus_tag="LR110_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL4821911.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26638..27624)
                     /locus_tag="LR110_000912"
     CDS             complement(26638..27624)
                     /locus_tag="LR110_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBL4821912.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8070 a         5242 c         5030 g         9357 t
ORIGIN      
        1 ttaaccacag ataaacattg ataacgattc gcttattgta atcaacagat taaatttcat
       61 atgttcatat gtacggatac attttcggac cgattaattt taaccattct gtttatttgc
      121 ttacctatta gatatcaata aaaaactgct taacagctat ttcagctgtt aagcagtttt
      181 aggattttta gttcatattc attgtttctt ataatacagt gttcaatact tctcttaaag
      241 cacttgttga tttgctgaac attgcttttt cttcttcgtt gattgataat tcaacaactt
      301 cacgaacacc attttcgtca acgattgatg gcacacctgt aaagatatct ttttcgtcgt
      361 attcaccagt taaataagca gatacaggca atacagcttg ttcgttattc aagatagctt
      421 ttacgatacg tgcagtactc ataccaatac catagtaagt tgctttttta cgatcaatga
      481 tctcataagc agcattacgg actttatcag caatcacatc taattcatct tgggcaatac
      541 gatggtcttt ttctacaatt tcaaacactg gtttaccacc gacagttgta tgtgaccatg
      601 cagcaacttc tgaatcacca tgttcaccta agatatagcc gtggacgctg cgtgggtcaa
      661 cttttaattt caatgcgatt tctttacgga aacgagttgt atccaatgtt gttccagtac
      721 cgataacacg tgatgttggc agaccagatt cgttccaagc gatatatgtc aaaatatcta
      781 ctgggtttga agcaacaacg ataataccgt cgaatcctga tcccatgatt tcttttacga
      841 tttggcgcat gattgacgca tttgttttaa ctaaatctaa acgagattga ccaggttttt
      901 ggttgatacc agctgtgata acaacgatat cagcatcttt acattcttca tagccacctg
      961 accatacagc cacattttca tcgccccaag ccataccatc taaaaggtcc aatgcttccc
     1021 cttctgcttt ttcttgattc acatcgatca atactaattc attagagatt ccttggttga
     1081 tcattgcata agcgatactt gttccaacaa aacctgtacc gacgattact actttacgac
     1141 ttgttttttt catgaataaa cagctccttt tcataactat ttgttacttt catgaaaagt
     1201 atagtcttgt ttattcattt tatcaacatg ttaattataa tagttgttat tataattttt
     1261 gatggaaacg aattcctgaa tacgcataac agataaatga caaatgttta cacctatatc
     1321 ccctgataat acaaccaaaa gtttagaact gaaaataaat ccaaaaaaat tcgctgcaac
     1381 gatataataa gacagcaatc gcacctaaaa caattttcct tcatatgctt tagtgaaaga
     1441 ttttcttctt aatgtttatt tctccattca ttgggagtac atgttgaata cttcttgaat
     1501 aatttgaata aagaagtcgt ttgtttatat ccgatacgat tagcgatttc ttccaacgaa
     1561 aaactcgttt cttgaatcag ttccaacgca aagtcgaatt ttttctttag aaccagttct
     1621 tgaaaggaag catcaaaata cattttcaat ttattactaa gatagaccgg attgtagcca
     1681 aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat gcttatcgat ataatctaat
     1741 actctaagca tctcattttc tgcgcatcca tctgctgatg aaaagctttg ttgttctaat
     1801 aaatgagtca attcgatcag taatagtgat gtatagagat ttaacgaacg gatcttatga
     1861 ctgtatgtat caaaataaat caacagcata ttttcaatca aatgcgtcaa acgacttgtt
     1921 tttccgccgc gaaaatgaat gaattgtgta tgttcccctt tttcttttga tgcatttatc
     1981 aaaaaagaag aaagtagatt atttgtgctt gccaaatgat tcaatatctc tgtagataag
     2041 gtttcttctt ttaataagat gttgattaaa atatcctctt gccctgtgta ttcgattctt
     2101 tgagtagctt ctttatcaat gatcaccacg tcactttctt ccatctctat cctttctcca
     2161 ttaataattt gtaaacatgt accgctatac atataattga tttcaacaaa atcatgtgta
     2221 tgctcgggaa cttccgcata tcgacggtgt ttactgatat gaatatttcc ctgttgaaat
     2281 agatcccctc ggtggatttt tggcggtgtc tgaagatggt aagctaagtt taaatcattg
     2341 atattcattc cttgttcacg ctgcagagtt tcgattgatt cttcgttacg caaaaaaaat
     2401 gtctaattca gctagttcca attgactccc tcctttttgt aagcggtttc tttcgcttat
     2461 tagttcacgc tgtactgcta ttatatgcaa agctattttt ggtttcataa agactttcta
     2521 tggttgggac atgaacttcc tgtgattccg ctattgagag aataaccact tcatactata
     2581 tttttgataa gcgcttacaa aaatgaaagg aacatgctta tgaataaaaa gatgatgaaa
     2641 ttttctctgc tgtcagtctc tcttattttg acttctgctg gtgcagtcac gtctgcaatt
     2701 cccagtatgt cagctactta tgccacgcgt tctttatctg ctgttgaatt attaacgact
     2761 gctccctctt ttatggtcac tatttttgtt ttgattagta gttttattgc taggaaaatc
     2821 ggacaaaaac aaactgtgat catcggatta gtgattgcat cgatttgtgg tatcctcccc
     2881 ctcttctcac aaagttacag tattgtctta gcttcaagag ctggactagg tattggcttt
     2941 ggattgatca attctttggc agtcagcatg attgctgcct tttatcacgg agatgaacga
     3001 gcctctatga ttggttttca aagtgcattt caaggatttg gtgcagcttt aatgacattt
     3061 gttgcaggac agctagtaca gttcggttgg caatatacct ttttagtcta cgccatcagc
     3121 ttacctattc ttgtcttgtt tgttttgttt gttcctacac ctgaaacaac tgaacttcaa
     3181 tcgcatggca cagccccaac aagacaagta atcaaaacga atcctaaaat gattagttat
     3241 ggtatttttc tcttgctggt catcgtggta tataatgctg tatcgattaa attagccaca
     3301 gtcctgacga cttattctct aggaaacgaa actaatgcag cgaatatctt aacaatcatg
     3361 caattggcaa gtatggctgc cggagtttgt tttggctggc ttcagacaaa agcacatcgc
     3421 tatatgctta cactttcttt actactgatg ggaatcggct ttgtattgat tgccactgtg
     3481 ataaacctgt atgtgattgg gtttggtgcg atcctaacag gaatttcttt ttcgttattc
     3541 attccttact tattcaacca agtaagtatc caagcgccca agtacgcgga gagtttcaat
     3601 acttcaatct tattggttgg cgctaattta ggtactttca ttgcaccata tggtttgctc
     3661 tttctctctt ttgcttctct tgggagtaaa acacttgctg ttttcgtcaa tggcagtatc
     3721 ttgctgttga ttagcgcgct tattagcctt ctagtagtac acaggacgaa tagaaagaca
     3781 gtgataagag tagatgaaga aataaaagaa caattgacaa caaaataaat ttgatggagg
     3841 tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc tttggtatcc tggagacttt
     3901 gagatccgcc atggactgct acaaaatttc caacgggaag aacgaggttt tgactggcca
     3961 gcgtactggt acatggatga ttgccatcgg aacgtcaagt ttaagagata ttactttctt
     4021 gatcaacctt ccatgtttaa agtgacgatc caaggagttg ggtatgtaga aattaatgga
     4081 caaaaacatc catgtggaaa atggctgact tgtccagctg gtaaagcaaa aattcgtatc
     4141 tttgtaggac atacatcagg gctaccagcg atgtttatcg agggagacga agtaaaatca
     4201 gatattggct ggacagcaag taattttatt gaagaatatc cagctggctg gagtccgctt
     4261 tatgtcgata tcgcaaaaga tccaaatcaa atctattatc aaaaagaatg cgtccatcca
     4321 gtcactgaaa cggagattgg cggaggaatc ttattcgatt ttgggcgagc agtgaatggc
     4381 tgtgtagaac taatatctct ggatgaccat atcgaatcta tcactctatg ttatggagag
     4441 tccgatgtag aagctatgga tatcgattat tgctactata agcaaatagg aatgacaaga
     4501 aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta taccagaggt ttctactgga
     4561 atggttcaac ttcaagtttt tcatgaattt ttatctcttt cgaaacaagc tgctttttca
     4621 agcgattctt ctttactgaa tcagatctgg tcagtatcag aagaagcctt caagttatgt
     4681 agtgatttgt tttttatcga cggtatcaaa cgagatcgct ggatttggtc tggagatgcg
     4741 tatcaatcat tcatgatcaa tcaatatctt ttttttgacg aggaaatcaa taaacgaacg
     4801 atattagcat taagaggaca aaatgaaatc aagcagcata tgaatactat cgttgactat
     4861 tcgatcttgt ggttgattgg tatcgaaaac cactatatga tgacgaaaga ctatgaattt
     4921 ttacgcatcg tctaccctaa gatgcaatca ttaatgagat acttgatgga gcaaacgaat
     4981 gaattagggt ttatttatgg gagagcaaaa gattggatat ttattgattg gtctgaaatg
     5041 gataaagaag gaacgcttgc tgcagaacaa gtcttattac tgaaagccta tcattccatg
     5101 atcacttgcg gagatatctt agctgaaaca tatggagaga acgaaaatta tcatactcat
     5161 ctctatcaag aaaaatatca actattgaaa caaaacctat ttgactactt ttgggatgaa
     5221 gaacttggcg cttttatcga ttgctatgaa tctgggagaa gaaatgtgac gcgtcatgcg
     5281 aatattttcg ctgttttatt cgattttgct aacaaagacc aacagcaatc gatcttaaag
     5341 aatgtcttat tgaatgacac tatcacacag attactactc catatttcaa attctatgaa
     5401 caagatgctt tatgcaaact cggacagacg aatatcgtct accaaacgat tcttgattat
     5461 tggggaggga tgttatctca tgatgcggta actttctggg aagaatacga ccctactcaa
     5521 acaggaaatg aaaagtacag tatgtatgga gatccttatg gaaaaagctt atgccatgca
     5581 tggggagcaa gcccaatcta tcttttagga cgctatttta tcggtttgta tccaacaaag
     5641 ccaggttatg aagaatttat gatacaacct aatctagatt catttgaaac actgaattgt
     5701 acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa caaaagataa gatcgtgatt
     5761 acttctgata gaacaggcgg aacagtgaaa atgaatggta aaagttatcc tcttctagca
     5821 aaacagccac ttgtcttaac aagctattca aaaataaatg cataaaacaa aaaactgctt
     5881 gcgacatatc agaagacttt aaatcttctt ctgatattgt ccaagcaatt tttattttga
     5941 agtttgctta attctgttct ttagaaacaa tgtttgttct ttttccggca aaaagaaagc
     6001 tagataatac gacaagtaaa aagaaagcga tgattccaac tgtcaaggct atttgaccgt
     6061 attctcctag tttcccaagt acaagtcctg gtactagata atcagtatcg gcaaatgtag
     6121 aagaagcgat tttaagatct cctaatactg gaaggataaa taatggcagc caactaatca
     6181 ataagctatt gaaagcagaa cctaataccg cccctcttct accgccagtc gagttgccga
     6241 agacaccaga agcagctccg cagaagaaat gtcctactac cccaggaatg ataactgtag
     6301 tttgcaagcc tatcatagca aacatggata gtgttcctac aaagaagcta acaaaaaagc
     6361 caattaatac agcattaggt gcatatggaa aaacgatagg aacatctaat gctggtttag
     6421 aatcgggtac gagtttttta gcaatccctt ggaatgctgg aacgatttct gccaacacca
     6481 ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa tgttccagct tggatcagtg
     6541 caaagattac agcgttgtct ccattgctta gttctctttc aacaaatcca ggaccagcta
     6601 acaaagcaag tgcgacatat acgatcgaca tcaagagcat gatcgataca gtagaatctc
     6661 gcaaaaagct gaaactttta ggaatcttta tgtcctctgt agaacggtct ttgtttccaa
     6721 aaaactttcc gatccatcca ctagaagcat aaccgatatt tccggtatgc cctaacgcaa
     6781 cttgattgtt tccagtgatg ttcttcataa acggttgaca aagagcaggc gtaatagtta
     6841 atagtgttcc ttcgaagacc ccgcctaata agatcatcca caaatttgca gtaaaccccg
     6901 cgcttactaa aatgactgct gttagacaag aaacataaag cattgcttga cctgtaagaa
     6961 aaatgaattt atatcttgtg aatctagcaa gtaaaatatt cacgatcatc cctactagca
     7021 tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat cgccactact gcttcattgc
     7081 taggaactac tccttgagta tgcagagctg tttggaacat cgaagcaaaa ggggttagtg
     7141 aattactgat taaaccagca ccgccgatca gtactaggaa tccgacaaat gttttgatag
     7201 ttccttggat cacttctgaa aatttcttct tctgaagaac gagaccaagc agtgcaatca
     7261 aagaaacgag gatggagggc tgactgacga ttgacacaaa caaattcaac aatttatcca
     7321 ttgatagttc ctcccttgaa tcatttttat tcttttgaca agatcttcgt tactttttct
     7381 tttacttctt cgctgtcaat gatcgattgc aaaggtacga gcttttcatc aggtatagaa
     7441 gttagcgtat ctttcaaaga atgctcaatg aagaaataat cagccatatc gctagataca
     7501 cttccaacat ccgtatgact tacttcaaac tcgtccacat ctgctccaag atctgcaagt
     7561 gcttgtttga tgttcatctc taccataaaa cttgttccta atccactttg acatactgca
     7621 aaaattttca tatcattcgt ctccttttct tatgatcgct aatatttctt cttttgttga
     7681 tgcgtgtaat aaatcttgaa gattttgttt attagaaagt agctttgtta aatttgccaa
     7741 agcccgaaga tgtgcctcat tatcgatagc agctaaacag atgaacaaac taatgggatg
     7801 ggctggatta tcaagcagat ccacaggttg ttgaagtttc agcaaagaca tccctacttc
     7861 gtttacgcca tcttctggac gagcatgagg taatgcgatg tgatcgccaa tatggataaa
     7921 cgcaccatac tgctccacgc gctcgatcat agctttgata taactttctt ctattttatt
     7981 ttctgataat agcggctgtg cggcaaatgt gattgcttct tgccagtttt ttggttgatc
     8041 cgataattgg atcatttctt ctgttaataa ttcagatagc ataggacgtt gatcctcctt
     8101 cttttctaaa agtttgttca ttttacgatt taatacttga tagagctttt cttcgtctac
     8161 gccttctttt aattcgatat aaggcagtag cgccgacaac aattcttggg cagaaggcat
     8221 tgaaaatcct ggtatcagcc aatctctttg tacttgattg atcagctgat ttttttccaa
     8281 gtcagtcatg attggatgca gaacatagag agactttttc ttatcagtaa gtgtgagagg
     8341 tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc tcgtgtaagc ggtcagctga
     8401 attagtctct gtaaaaaaga tcatcgggaa taattgctga agttcacttt ttaagatcaa
     8461 tgaagagcta atgccacttg gacagacgat cacggctctt atttttctat ttctttcttc
     8521 tgcgtccgct tttctgatct ctccgccaaa tagtatagtg aaaaaaccaa tctcttcttc
     8581 agggatgctc ttccctattc gtctttctaa cggaagtaat gcttttcttg tcatttcgta
     8641 gatggaagca tattgatgct taatttgatc gatcatcaca ttaggtaaat aaaaagaata
     8701 ggaaatacga aaataggcag ggaccaagtg ataaaagaca ttcatcaata attcacgaaa
     8761 cgaagcaaat tctacggcca tcaggaattc catgcgatgg atgacttctg aagaacattc
     8821 cagcaaaaaa tcaagtgcag gctcctggac atctccttca acgatcgatg ctaagcaaag
     8881 agagaaaaat ataatctccg aattttgatg ttcggaatag ccaaggccag tcaatacttg
     8941 tcgactaatg tcagtgactg caagagaagc caacagttca gcatctttta ccagttccat
     9001 ggatgttttt ccaattcgaa tggttgtcaa actcaaataa taagctaatt catccattcg
     9061 atttggtgcg aatcgaagat ctgatagtga aatcgtttta tctagcaaat ctctaacttt
     9121 ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg agcccaaatc ttccagcaag
     9181 tgactgcatc aaacgagcag tgcgattttt tgcttctctt cttaatattg cttctgaccc
     9241 tagcagccta aaaccagttt tcctagtata cgacagctga caatcttcca cttctaattc
     9301 atttcttatc tttttgatat ctgacaggac tgtcccttta gaaacctgaa gaaaatcttg
     9361 aaaatgaaaa acagaaacag acgttgcagt cgaataaacc atcaaataaa gaagatctct
     9421 tcgctcttgt tcggtataca cagcttcaaa tcgagtaata tccaaaaatt tttctgccca
     9481 gtcgattgct cggaatttct ttatacggat acgggtatca tccacatatt cggcaaaagg
     9541 agttaattgt ttgatcgcct cgcttgtatt tacttcgatg atctctgctg ctttttctag
     9601 actgatttct cttggtgttt tcaggagata actaattttg tagttcacag cgctgttcac
     9661 ctcctactta tattctagta tggtagcgct tttaaatcga ggggtgtttg gtacaactta
     9721 cagttggatt ttttttacga ctgtaccgaa cgatttctat cgaatggata cagaaaaaaa
     9781 gcaggtgacc aaatgtctcc tgcttttatg aattgatttc gtccgatcaa tgatgatcat
     9841 gtttattttt tgaggtcagt tcttcaaatt tttctttgac atcttcaacc gctttttcga
     9901 tatctttttc aaagtgattg ggctcattag gctttgtttc ctcttctaca gaatttgaac
     9961 cagataaagc tgtttcttta ttttctgttt caccagagtt tgtgccagct aatgattctt
    10021 ctttgtcttc tgtttcacca gaattagttc ctgctaaagc cacttcttct tctgttttag
    10081 gggccattga acctcttaat cgtttgtgtt tttcaccgtc ataatttgtc atgttggttc
    10141 ctcctagtag ttctatactt tttcatcgta caataagaca gtttatgcgt caaaaaacaa
    10201 acccgattct tacaaatatg tcatattttt ccttctgatt cttgaatgct tttgattcct
    10261 tcttccaaaa ttttcatttg taagattgtt tcttcatctt tgatgacttt ttctgcgcca
    10321 ttaactaatg tctcgtaaat cccttcgtag acacggctgt aatcaccaac ctcggaaatt
    10381 actttttctt catgataact cccttgttca tctatatagg tcaatacacc ataatgttcg
    10441 ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa acattttcag atgttcttct
    10501 tgtcgatctt ttgtttgttt cacgaacatc cctttttttc cgtaaacgac gaaacttgga
    10561 cgttctttga tgcggaaata actggatttt atcgaaattt tcatatttcc atagaaaaaa
    10621 tccagatcga agtaatcatt cattcgtcct ttccctaaaa gttgacgtac atcatagctg
    10681 atatcatctg gttcgccgaa ataagaaaga acttgatcta ctgtatgaca agcatgtcca
    10741 tataaatagc tgttcgcagt agaaaattca tgcgtatttt ctggtacttc cggtctgaaa
    10801 taatcataat gcatttctac ttctaaaata tcccctaatt ttccgctttc gattactttt
    10861 tggactgtca agaagtcaga atcgaaccgt ctgttttggt aacattggat aaatagacct
    10921 ttttcttttg ctaatgcaaa taactcttct gcttcctttg acgtttctgc aaaaggtttc
    10981 tctactagta cattcttacc atgttcgagt gccatttttc catattcata atgaaattga
    11041 ctaggtgtag tgatgacaac taattggatc tcaggatcat cccaaagatc agacagctga
    11101 tcagtatatt cgatgtcttc gaatgttttc cacactggtt cagatgggga aaaaattttt
    11161 ttgatattga ttttgttcgt aagttttctt gaaaaaggca aatgatagcg atttgtactc
    11221 tttccattgc caagataagc gattgtaagc atgagtggtg cctcctttta ttgatgtttt
    11281 cagtatatcc tagaaaagcg cagaaagtat ttataatagc tattctggta aaaaagttca
    11341 gaaaaacact tgttatcatt cggtttggtg aaattaatgt tcaaagtaag gataaaaatc
    11401 aaaaagatag tagcggaacc aagtgtttat cagtaagata gaatttggag gtcagatcat
    11461 gcttattgaa gaaaaactaa caaaacaaga gttgtttacc acgacagaga aacggattgt
    11521 cgattacatc cgtaggaata tcgaagctgc agtctacatg acgattgaag aattagcaaa
    11581 agctacgtat acttcccatt cagctatcat tcgtttatgc aaaaaaatgg gattcagcgg
    11641 atttaaagaa ttccgtttcg agctggggcg ggaagttcat caattgattg ctcagttcaa
    11701 tcaaacgatt gatcctaatt ttcctttttc aagtgaagat agcactcagc aaattgcaaa
    11761 gaaaatggcg gatttatcga tccagtcaat aaaaaaagca caattgcaga ttgaacatga
    11821 agatttagat caaatcggta aagtattgaa caaggcacag cgaatctttc tttttgcaaa
    11881 aggtgattct caaatcacgg caaggaaatt ccagaataaa atggtcaagt tgaataagtt
    11941 tttgatcatg gctgaagaat acagtgattc ttcttggaat gcagccaatt tgactagcga
    12001 ggattgcgcc atttttatta gctacagcgg tcgcatccat cattatgaac gaataatgac
    12061 ctatttgaag catgtagagg cccctacctt gctgattacc ggaaatcagc attcagaaat
    12121 ggccaaacag gctgatatgt gtcttgttat atcgcaggag gaatatgatt ttgctaaagt
    12181 agcgactttc tcctctcaaa ttgccttcga ttacgtatta aatacgttat tctcagtgat
    12241 atattctcaa aattttgaag agaatatccg caacttgaaa aacaaacaag aattgatgca
    12301 aaaaggcctt cttaaagaat atcggaaaac ttaagaaaag atcctcattt agtaataaga
    12361 aagatagtgt ttgataggct ctctttgata tcatcataaa aacaggctga agttttcttt
    12421 ttcaaacctc agcctgtttg tttacggaac gattcgaata gatatattat agaaatgagg
    12481 aatagctaac ttctgtttct tattttgaac gattgacgaa aaagatatat aagaaggagg
    12541 aaaatcagta tacatgcagc taacaatcca tacaggaaaa atcgtttgtt ctcggatact
    12601 ttctgtactt ctttttgaac ttcttttgca tgataaggaa cccggtgccc tctgacaagt
    12661 aagcgatgac tgttgaccat ataaggtgta caagttagga gtgtcacgaa atcctgccct
    12721 ttttcgattc tcagatcttt tgtatcagtc ggttctaccg tcttgatctg atcgacttga
    12781 taagcgagtg tccgtccatt gatttcgata tagaaagaat cacctaaagt cagtttcggt
    12841 aagtctgtga acaactttgc ttgaggcagt cctcgatggc tggaaataac ggcatgtgta
    12901 ttttctcccc ccgtaggata agacgttcct tccaataatg aggcaccttt ttctagcagc
    12961 acagtattcg tttcatcaaa aattggcaaa tggacattga tctcagggat cgtcaaaata
    13021 ccgatcgtat gtttctcaaa ataggttcta tcctttttcg caggttcttt tttcttagta
    13081 aagggatctg cacctggatt tccaccttct tttgctaatt gctggttttt ctttgtcatg
    13141 ttttcttgga ttttagccat gacttcttca ttttctttga ccgcttgttg ctgataatga
    13201 gagatgatct gctgatccag ataattattc agcgcgtcgc taacaaatgg ataagctagt
    13261 atccctattc ctgagagaag caagaaaagc atcagcagat ctagaagttt ccttttcttt
    13321 ggtttcattt gtgttctcct tgtgatgact gtttgataat aaaagaaaaa ggagcatttg
    13381 cgtaatccgc tgctcctttt tccaattctt gcaaaaagac taaatctgac tgtgcttgcg
    13441 tctagcaaag tacagtccag caatcagtag aaggactact cctgcaccga tatacacata
    13501 gattccctta ccgcctgttg aaggaagtgt acctttgtgt ttatttggta ttttttcagg
    13561 agaaatcaac tgtcctgctg ttacataaga ttgttcatcg atagtaaatg ctacacggtt
    13621 tgttaatgga acatattttt ctggcgcttt cgtttcttcc agatagtacg tgccatattt
    13681 tagacctgtc acatcgatta aaccgttact tgtggttgta aaaaccgttg ctgattcttt
    13741 cgccgatacc cagctgacgg cttttgtgga tgggtcgatc gataaatatt tcgctgtgtc
    13801 actatcttga tcacgaacga cgaattctgc tccagcaagt gtttggtctg atgtaacgtc
    13861 accatctact ttaacaaatc gtttgccccc agtaacgaca tcgactgacg gtggtgtttg
    13921 atcatttgta tgcccgttat cgacatttgc ttggttagaa aatcctttgg ttggatctgc
    13981 tttttcgttc aaatgcatat agtaaacgaa tttcaatgta ccgccaggag ttaatgaagg
    14041 aatataattc ggatcaactg aaaccgtgaa tccgtctgtt tgctcagtga cagaataatt
    14101 tactgggtcg atttctttat ttccatcata taaggcataa gcatacgttc ctgaagaatc
    14161 attatcaaat gttaaagcag cgtcatgagt atcgataagt ttgaatgttg tgtacttgtt
    14221 ttgcgttcct tctttatcag cgatacctaa tgggatattg acagaaatct catattgaat
    14281 tttttcaccg attgcgacat cttttccatt caattgaggt gttgttttat caactttaga
    14341 cgtatcattt ttgatggtct tttctactgg tgtttggcta tttgccagga tctcaaaagg
    14401 cgtgattgtt tgcttttcaa tcatttccgc attatcagga gcttttactt cttctaaatt
    14461 atattttcca acttctaaat gattaacgat caactggcct ttttcaatag atgcctcggc
    14521 aaagtcattg ttgccgatgt cataagaatg accagtaatg aaatgtttgg ctttggtttg
    14581 atcagttgtc caagtgtaca atccatctgt gacactttgg atgtattttt tgacgcttgg
    14641 tgttccttct tctttagaaa taataaattc tgctccattt agtgcttcgt tttcggcagt
    14701 accgattttt gtaactttca acgttccatc attaccgact gtatttttag ggtagagatg
    14761 gatagtatct agttcttctg tcccgtattt ataagagcca tctgcttgtt tgatcatctc
    14821 atatacaggg aaagctaaaa ccatgtttgc ggcagctgac actccgtctt ttggttcttc
    14881 tttgatcgtg tagactgcat ctttcccatt ttgtttttta ggtaaagata aagtgacatt
    14941 tccatcagca tctgtcgttc ctgaagcaac tggtgtacca ggagtcaaag actggactgc
    15001 ttgttttgct gcatccacgg acgctccttt atctcgttgc gcataaaatt cttgagtgac
    15061 gttataaact gaaaatgaaa tatcggctaa tccttggtat tgatcgaatt cgctcatttc
    15121 tttcccgctg ttttggatta aaggatcggg taaatcagtc attttctttt tatgaaggat
    15181 gacttgagct gctgtctcct ctgcaaacac tttttttgcg ccgaagctgt ttgtgagtaa
    15241 tggtaaaata aggaaaagga ctcctaacat aacgtttatt tttttatgat ttttcatttt
    15301 ttcactcctg ttcttttttt gataaacaac cagattgcaa agcttgcgaa taccatacca
    15361 acaacgccta aaagtgtatt tttcgtttcg tttgtttttg gaagaaatgg acggctattt
    15421 tcttttttat tgactacttt caatatactg tccgcctctt tatcctcaga aagaaaagtt
    15481 gaaatggtaa acggaatttc tgtttcactt aaaatataac cagaaggtgc ttcgatctct
    15541 tttaatcgat aacttcctgt tttcaacccg gaaattgaaa aagcgccatt tttatcggaa
    15601 atcaggtgaa gcgcatctga ttcgttcttt gtccacttgt atccgttggc tgtttgctgg
    15661 aggtaatttc ctgctttgtt tttaataagg aatactgcgc cagctaatcg tttatctggt
    15721 ttatttgtct cgattttgac aaattgctta ctccctgtat atacagagac tttctttgta
    15781 ctgacaccaa aaccaaaatc cagtgttccg ttgttgttga tttccttgtt cgcctgtgct
    15841 gtactgctta gctgcatctg ataagaaatg gtcagttttt ttccagcata tttttccaac
    15901 gaagggatcg aaaataaaac atcaaatccg tgaggtttct tgtgtatgac aaagccttct
    15961 gtcagctttt ctccattcga tgaaatcgtt aaactattag gtaaaaacgt taatgcttca
    16021 tccgcactat ctgacaattc gaacttttga tagtcaagga tatttacagg aattgtcgta
    16081 tgcaaggaat aagtgatttt ttcaccaatc gtaaaatcat ttctaggctc ttctaatacc
    16141 ttctcaaaag gtggatcagg gtagtcgttt tcttcatttt taggatacaa atggatagtt
    16201 gaaagttttt gattgttttg tccatgtaca ggaagaacaa ctaccatatt ctctgccttt
    16261 tctttgacga cttctggtac tttggattca atgaaaagat acgctttatc tcttttttct
    16321 tgatctttag cggccagaga aaaagaagcg ataccatctt cgttgttaag agttgtagtt
    16381 gttgcttctg cggtaaacat accggaagac gcaccgtttt ttgcgatctc tgcttgtgct
    16441 tcttctaccg ttttgccctt ttcccgtaga tggtaaaaag aatctgtgac atcataaact
    16501 tggaatgtga caccatttaa tcctcgatac gtttgtaata aagctttttc ttgtccgtca
    16561 tttggatgat ttttcggcag ttgcccgttt gggaaaagca atttgtgtaa agtaatctga
    16621 accatttctg tttctgtttc tgttgctacc tgagtaaaag ctggtttaaa tagtaacaag
    16681 aagagacaca tactaagcca accaagtttt ttcatattta gctgccccct ctttcttttt
    16741 tcaaaaaaag ataccaaata gaaaacgcac aaccaatcat ccctactaga tagattccta
    16801 aacggccaat tcctcccgtt tcaggtaatg gtacttttgc ctgattcgtg atgtctaaag
    16861 aaatctggtt gtttttggct cctggtgaca gaacagattc atgatcttgt ccatccactt
    16921 gaattgaccc atcttcgtgt ataactatag taactggctc ttttagacct tggtatcctt
    16981 ctggtgtaaa agtttcggtc agcgtataag ttccaggttt taaattttcg aatagaaagg
    17041 tatctgtttc ttgtccatct tttggcgatt ctaagtccat ttctggtccc tgcagtctga
    17101 attttgctcc ttttaatgtc tgtccgttgt cttctttttt gtgtaccgtt agatcaaacg
    17161 cttttagtcg attttgatga gtgagcgtcc atacattatt tgtcatctcg gtattttttc
    17221 ccgtgtaaat gatttttcca tatttatcga tttggaattc ataatttccc ggcgaagtat
    17281 catatccggc aggaccagct gtttcaacga ctcgatattt accaggtgtg agtgaatcaa
    17341 aactggcaag acctttttca ttcgttgttt ggctgtcaat tggctgataa gtaccatttg
    17401 cttctttttt ctgtagggaa aatgctgctc ctttaagatt tatctctttg tccgtccctt
    17461 gcatcgcata cttacgtact gctacaggaa cttcaacaaa aggattttca atagtcaact
    17521 ggatcgtatc atcggaagta gtgactgttt ttccatcaat ggttaccgta ccatcagaag
    17581 cgatcttgat ttcccaagta gtctttttgc ttaatccatg cccttctgga gcttttgttt
    17641 ctgtcagcgt ataggtcatt tctttttgca atttgacatt ttctggaaga gaataggttc
    17701 cgtcgccatg atctgttagt aaagtatcaa tagaatctcc tgttaatttg aaaacagcgc
    17761 caataagatc cttttcacct gtagaggaat ttttcgttat tttcaagttt aacggtgtga
    17821 attgtttagt atttttccat gtcattgcat ctatttgttg agaatcgtat cctggtacag
    17881 gtaattcttt gattgtttgg taactgaatg cttgaccttg attattgtat tgaggtagtg
    17941 ataatatctc ttgataactt tctccgctat ttgcagataa tttgtcaatg tctgcacgtt
    18001 cccacgtatt tgttgtatct ttagctggtt taatgattcg aatatatccg tttttccaag
    18061 ctgcagcatt tgtcgtatgt tcccgttgaa tctcaaaagt aacttgatct ggacgatcag
    18121 ctagattgtt gtcaaattct tcccataact ttttgatgtg aagactgact ccgggagctt
    18181 tagcagatgg tataccgaat tcagctaatt cattggtatc aatgtttggc tggaaagttg
    18241 tcctgccgtt catttgatac cagaaatttg gatggaagtc ctcattttct gtttggattc
    18301 tcacttggta atggatttgg atttcttggt cttttcctaa atagatctga ttgctcttga
    18361 tggtatttcc ttctatggaa atagatggag tgaccgttgt aggacttgtc cccacactct
    18421 tgactgaaag tgtaccaggc tgataaatga atggttctgc gattggatca tttatttgtc
    18481 cattgcttac agttgctgag atctgtacag cttttttggc taggtattca gagatatctg
    18541 ttgcatgatc agctgattca tagtaaagat cgcctttttc atctgatgaa accatttgtc
    18601 gcatacgaga ctctacttct gcttttgaga gaccagctgc cggatcgctt tgaagttgga
    18661 tgccaagacc atgtatttcg attcctcgtt ctttgagtgc catcgcttct ccgatggttg
    18721 cgataaacgt actgtcgatt cttctggata gattgttttg gtcaggtgca tcatagattc
    18781 ttgagattag agaagtattt cccggccgat cttgcgtatt agaaaactga gttccgtaat
    18841 aattgctgct tgattgtgcg tgtacccgct gtactttata ggaaaatgtt ggtacaccat
    18901 ccgtcagcaa aacgatcact tttttatgtc catttggaac ggatagcatg cttcctgcat
    18961 ctcttagtgc tttttgtgta aaagtaccac catttgtccg tgaaggtgta atggatttta
    19021 cttgattttt cactgaatca aatgaaccca tctgtactgc accgttactg tagctatatc
    19081 cttcgcttga gtagccgaca tatcccatat tgattttgtc tgtgataccg ctatctgcta
    19141 aagtatcgac aaaacgatcg acaccaatct ttacttcacc gatccgatta ttgtcgttca
    19201 tacttcctga ccagtctacg accaatacga gatcaagagg cgtgatatct ttttgtacat
    19261 ttccacgagc attcaaataa acatcaaaca atccgggtgt acttgtttcc tttgcatact
    19321 ttcgtatcgc atagtctgct tgattaccgg ttcctccata ttcgatatac gaatgggtcc
    19381 gatcattagg atctccattc caagaattta taccatccca ttgatttgtt ccgtttttat
    19441 tgccttgatg atcaagaaca tttgtatttc ctgtaggttg ccaactggct ttaggataag
    19501 tgccctgatc atctgttgta tattctgtag gaatcgtttg attagatggc acactcacag
    19561 ctggcaagga taatgtaggc aatgtaaatg ggctgacaga attttcgttt tcttttgatg
    19621 attcttcttc tatttcgtct ggctttggta ctgacacatc tgtatctttc ttattttctg
    19681 tctcatcaat aatgtcatat tctttattat cgacgatcag cttgtttgcc tggctgtggt
    19741 aaagtgctgg atcgattgga aaactcagat cgatagtttg tttagtgttt gatgagattt
    19801 ccaacgtaat tttcccgcta tttacctgat aagcatagcc atctccttct tttctttcca
    19861 catcacttaa gtaattttcg actagaccaa tttctttttc tatcttttca gcactggtgt
    19921 tattcgcatt tagcttgatg ttcagtgctt tttttccaga ctgatccgaa atgacttcag
    19981 ctgaaattgt ttctaaaact gttggatcat ctgtagtgtc tgctattgca gataatggca
    20041 caaagtttga taaactcaat attatcagca tgaaaataac tttcagtttc ttccctgtgg
    20101 tcgtcatttt tcccctcctt tttctttttt tctgttttga cgtgaatagt gaaaattatt
    20161 aaaataaaca ggcgttacat aattttattt attttaatat gataattatt atacgaaggg
    20221 cgctaaaggg aatcacacca attttcatac caattggttt catttatgaa gttgattcct
    20281 tcgattaatg agcgtttcat tgaaagaaaa gggttgaggg agaaaaacat atgatcaggt
    20341 tgttggaacg aaactataga aagatggaaa aaatcattcg aattctgtcc atttctgaca
    20401 agtggatgac taaaaaagaa cttgccaaac gaattggtag ttcagaatct acatttgtaa
    20461 ggtatatcga agagatcaaa atccgctggg gaacggatgt aacgattcaa tcttcacaaa
    20521 aattggggta tcgtcttgaa catttgaatg taccagctta tttaaatgtt ctgatggaaa
    20581 tgtcgcaaag ttctacaaac agtcgtttat taaatgagat tattgtcaat ccgggtaaaa
    20641 cgattgagta ttattgtgaa aaaatttcca tcagccggtc ttcttttaca cgtaagttga
    20701 aacaatgcaa tcaaatttta gaaagctatt tattaaaagt gatcgttgac caaggttttc
    20761 agttaactag ccttaaaagt gagctccagt tgcgtatctt tgttaccttt ttctttttaa
    20821 gttactacgg aagacgcacg ttgccatttc caatcgaaaa aaagaagatc aaagaaatta
    20881 tggtcaagaa taattgtcag atgaatttgt ttcaggaaga aagcccatat gagcaatctt
    20941 tctttattat gtattttgtt gtgcatctca tgagagaatc ccaaggaact ccatttgaat
    21001 ggaaagctgg agactcttat gtacagttga taaatatcga taaaaaagac ttcttaatct
    21061 tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat aaaagtagtg aactattttg
    21121 tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa tgaaatcagt gagcagatac
    21181 gaaaaaatat gtcctactgt tcgttttcat ccgctgattg caaagaaaaa aacgaagaat
    21241 atgttgttca attattaacg caaatttatt ttttctcccg tattttccca tacgatacga
    21301 ctcatttgac gatccgtgca acttactttg cacagcagat gcaacaccgc cagatacgtc
    21361 tatatgctgg atttcgtgca gaacttcaat tttattccac tctactgaat cagaatctga
    21421 tttattattt atctagctat atcttttgga tgcttatcgg taatcctgaa attcaccatt
    21481 atacttctga taaaaaaata ctgattatca gcgacttaag tcttcgacat agtcaatata
    21541 ttgaagaata tatcagtgat attcttgctg tgcataaaat tcactctgag acaactgcta
    21601 ttactgaaga tcagctgtcg aaatacaatc ttctcgagta tgatctgatc gttacgaatc
    21661 aacccatttt gaatgcccat gttcctcata tattgattga tgatagtgtg tcattcgcaa
    21721 atgaagaaga actcatccgc ttattcgagt tatgaataat caaaaaacag ctatgtataa
    21781 accaccctga taaaaatttc aggacaaata cacatagctg tttcttttta ttgtattcct
    21841 tcagcaatcc aacgagattt atagcgaaag tcttttaaca actcttcttt tgtccaatta
    21901 tttgcaatga gatccgtcaa agtgttcatt acgagggtca tggaaaagta taggaaacta
    21961 actgcttctt ccgacgattg aatggagaaa tgattgattc ttaataattc cagccatttt
    22021 tcgttcattg gagaatcagc cgatagttct ctacgtttgg ttgtctgctg atcatttcgc
    22081 tgggttaaca agtataacct ttgccaatag atactttcgt gatctaagtc gcagcaccac
    22141 tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat gaattttaag
    22201 atatcttgat ggactatgtg agaactgctt ttgatcgtat acaaataagc atcttccaaa
    22261 tcttgaaaat atttataaaa agcaccgcgc gacatcccca tcttgtcaac gatttctgag
    22321 actttgactt ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc atcgattctc
    22381 tgtttctttg cgttcgataa attgaaaaaa gtttcttttg gcatccacat ctctccttta
    22441 tctctgatta tacgtggata gcccttcatt gtcatgaagg attcattcct tctttttctc
    22501 ttcttctgtc aagtacccat cttccattcg ataaacgcga tcactccatt gaatcatacg
    22561 gacatcatga gtaaccataa tagtcgcttt ttgtttctca tgagcttctt taactagtaa
    22621 tttgacgact tcatatgcat gatctgtatc caaactagct gttggttcgt ctgctaagat
    22681 caagcttggc tcattatata aagcgcgagc aattgccact ctctgtcttt ctccaccaga
    22741 aaggtcttta ggataactat ttttaagtga ctggatatct aaagattgca gcaaggaatc
    22801 gatttttgtt gaatcttctt tttttgaagt aacttggtca actagttcaa attgttcctt
    22861 taccgttaaa aaaggaatca aattagagct ttggagaata aatcctattt ctttaaagcg
    22921 taaatctgct cgtttttttt ctggaagctc agttagtgag tgaccgttga tcactatttc
    22981 tccggaagtc ggtgtttgca atcctcctga aatggtaagg aaagtacttt tacctgaacc
    23041 agaaggacca atgatgctga cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa
    23101 agctgagacc tttgtatgac cagaaccaaa tgttttggta acttttttca tcgttaatat
    23161 ttttttcatt tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt
    23221 atcgaaaaca atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat
    23281 tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca
    23341 aacaaaagag caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat
    23401 ttagcgatga agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc
    23461 tgaagtgtca tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag
    23521 tatatcatcg catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat
    23581 gtcccaatag ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa
    23641 tcctttatta cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca
    23701 tacttcaaag cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa
    23761 gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta
    23821 tctccaattt tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt
    23881 ttaaattcat gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa
    23941 actgtaacat tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct
    24001 ttctctcctc cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact
    24061 tgatttgcat cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta
    24121 aactcttctg aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag
    24181 aacataatac cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct
    24241 agaaacattt gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa
    24301 tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg
    24361 tctaagacat ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat
    24421 ttatgctttc catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact
    24481 attagtagta gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat
    24541 cggcgagata acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt
    24601 ttatgatctt gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc
    24661 aaaaatacgg aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt
    24721 ggaagataag attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta
    24781 tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga
    24841 tgaaaatgtt ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta
    24901 taaagaacaa gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca
    24961 aaataaggga atcattttga caaaagagca gatatttgac cggatctggg gatttgattc
    25021 tgatacgacg attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag
    25081 tactggattg gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt
    25141 gtgatgcaat gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg
    25201 tagcagcatt tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc
    25261 aatctgcata cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag
    25321 tacaattaga gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg
    25381 aacaaaacag aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca
    25441 ctcaagtgat cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc
    25501 gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata
    25561 ttgaattaaa tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag
    25621 cggagacgaa tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca
    25681 agaattcatt agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc
    25741 tctctattgg tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt
    25801 ggagaagaca aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga
    25861 ttattcaaaa tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat
    25921 ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt
    25981 tgctgacaat cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata
    26041 cacaagaata tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa
    26101 aagagtttat tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc
    26161 atcaagtttt ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga
    26221 tcattttgga ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc
    26281 cttctatcag tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat
    26341 ctcgggcgaa agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg
    26401 aacagcatca tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag
    26461 tccaattgcc gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa
    26521 atcttgtcac agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga
    26581 aataagccga aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta
    26641 cgatttttta ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc
    26701 tcggctaaca tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac
    26761 ttcttcacga ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt
    26821 aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt
    26881 gcgttcgatg acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac
    26941 cacatgtttg tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt
    27001 ttcttctttt agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt
    27061 cttgcttgta cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt
    27121 catattcaat ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc
    27181 tgttccagtg ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg
    27241 tgtatccatt gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa
    27301 ttttccatca gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt
    27361 ctcagaatca taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc
    27421 ttcttcacca tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa
    27481 tcctagtcta gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc
    27541 ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat
    27601 gaccgttaaa tttttctctt ccatgtaaat tcctcctagc aaatagattt atgattggta
    27661 tattgttact tataaattta ttctatagga gacgattgt
//
LOCUS       DAFFSV010000023        27666 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFFSV010000023 DAFFSV010000000
VERSION     DAFFSV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.023"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(229..2121)
                     /locus_tag="LR110_000913"
     CDS             complement(229..2121)
                     /locus_tag="LR110_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4821913.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2118..3845)
                     /locus_tag="LR110_000914"
     CDS             complement(2118..3845)
                     /locus_tag="LR110_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4821914.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3861..4511)
                     /locus_tag="LR110_000915"
     CDS             complement(3861..4511)
                     /locus_tag="LR110_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL4821915.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4715..5011
                     /locus_tag="LR110_000916"
     CDS             4715..5011
                     /locus_tag="LR110_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL4821916.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5183..5803
                     /locus_tag="LR110_000917"
     CDS             5183..5803
                     /locus_tag="LR110_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBL4821917.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5895..7061)
                     /locus_tag="LR110_000918"
     CDS             complement(5895..7061)
                     /locus_tag="LR110_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL4821918.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7073..7426)
                     /locus_tag="LR110_000919"
     CDS             complement(7073..7426)
                     /locus_tag="LR110_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL4821919.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7554..8576
                     /locus_tag="LR110_000920"
     CDS             7554..8576
                     /locus_tag="LR110_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBL4821920.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8595..8756
                     /locus_tag="LR110_000921"
     CDS             8595..8756
                     /locus_tag="LR110_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821921.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9015..9449)
                     /locus_tag="LR110_000922"
     CDS             complement(9015..9449)
                     /locus_tag="LR110_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821922.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9559..11418)
                     /locus_tag="LR110_000923"
     CDS             complement(9559..11418)
                     /locus_tag="LR110_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL4821923.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11536..12696)
                     /locus_tag="LR110_000924"
     CDS             complement(11536..12696)
                     /locus_tag="LR110_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBL4821924.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13003..14427)
                     /gene="araA"
                     /locus_tag="LR110_000925"
     CDS             complement(13003..14427)
                     /gene="araA"
                     /locus_tag="LR110_000925"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBL4821925.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14443..15141)
                     /locus_tag="LR110_000926"
     CDS             complement(14443..15141)
                     /locus_tag="LR110_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL4821926.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15098..16759)
                     /locus_tag="LR110_000927"
     CDS             complement(15098..16759)
                     /locus_tag="LR110_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBL4821927.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16977..18377
                     /locus_tag="LR110_000928"
     CDS             16977..18377
                     /locus_tag="LR110_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBL4821928.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18629..19708)
                     /locus_tag="LR110_000929"
     CDS             complement(18629..19708)
                     /locus_tag="LR110_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4821929.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19794..20753)
                     /locus_tag="LR110_000930"
     CDS             complement(19794..20753)
                     /locus_tag="LR110_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL4821930.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20765..22276)
                     /locus_tag="LR110_000931"
     CDS             complement(20765..22276)
                     /locus_tag="LR110_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL4821931.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22298..22861)
                     /locus_tag="LR110_000932"
     CDS             complement(22298..22861)
                     /locus_tag="LR110_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL4821932.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22867..23730)
                     /locus_tag="LR110_000933"
     CDS             complement(22867..23730)
                     /locus_tag="LR110_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4821933.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23744..24640)
                     /locus_tag="LR110_000934"
     CDS             complement(23744..24640)
                     /locus_tag="LR110_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4821934.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24815..26104)
                     /locus_tag="LR110_000935"
     CDS             complement(24815..26104)
                     /locus_tag="LR110_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4821935.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26076..27593)
                     /locus_tag="LR110_000936"
     CDS             complement(26076..27593)
                     /locus_tag="LR110_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL4821936.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8080 a         5840 c         4784 g         8962 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt aaaatcagcg
       61 gtttgacctg agtattagag atacaaaacg gaaaaatagc ttctcatatt ttgccgtttt
      121 ttgagctaat accgaaggac agctgattga acaccgttta ttcggaaaaa gggactgtga
      181 cacgactttt gtcacagccc ctaaaaaaaa taataaattt gtactattct atgaagcttc
      241 ttcttcagaa aactgactgt tataaagatc tgcatagaat ccgtcttttt ccatcaatgt
      301 atcgtggttt cctgtttcta cgatcgaacc atgattcatc acaataatgt tgtctgcatc
      361 gcgtattgta gacaagcggt gagcaacaac gaaacttgtc cggttttcca ataggcggtt
      421 catcgctttt tgaatcagta tttctgttcg tgtatctaca ctagatgttg cttcatcaag
      481 gatcaggaca tctggatttg ctaagaatgc ccgcgcaatt gtgattagct gacgctgccc
      541 ttgcgaaata ttgcttgcat cctcgtttaa tacagtgtta tatccttctg gcaatttacg
      601 aacaaaatcg tctacgtgag cagctttagc agcctcaatg atttgttcgt ctgttgcatc
      661 gtcgttccca tatttgatat tgtcatagat acttcctgta aacagccaag tatcttgcag
      721 taccattgaa aactgtgcgc gcaattcatc acgtgaaaga tcacgggtat ctacaccatc
      781 gtaacgaata ctacctccag aaacatcgta gaaccgttca agaaggttga tcagtgtcgt
      841 cttccctgca ccagtcggac ctacaatagc gaccatctca ccaggtttga catccaagtt
      901 aaaatcagtc attagcaagt tatctggtgt gtagccgaat tgaacatttt caaaacgaac
      961 tttgtatggt gtatcttttt cttctggaag gccagatttt tcattggaca tctcttcttc
     1021 atccaacact tcaaataccc gttcggctga agcaactgtt gattggatag tgttcatcaa
     1081 acttgcaagt tgagtgattg gttgagagaa ctgtgttgta tattgcaaga acgcttgaac
     1141 atcccctaat gtcatgttcc catttgcaac ttttactccg cctagtacag cgacgaacac
     1201 atagcctaga tttttaacga aattcatcaa cggcatgatg attgccgaaa taaattgagc
     1261 tttccaacca gcggcataca gattctgatt ttctttttca aatacttcct ggtctttttc
     1321 agtatgattg aatgttttaa caatggtatg accaccatat gtttcttcta cttggttatt
     1381 caataaacct aaactttttt gctgatctgc gaaaaacact tgtgatttcg gcgctacgat
     1441 catcacgatg atcaagctta aagggatcat caataacgcg actaacgtca attgccagct
     1501 gattgaaagc atcatcacga tgaccccaat caatgtgact gcactagtta caaattgagt
     1561 caaattttgc tgcagcgtgc ttgcgatgtt atccatatca ttgattgctc gagacatgat
     1621 atccccattt gtatgtgtat cataatagga aatcggtaca cgattcattt tggcttccaa
     1681 atcatgacgt aattcataaa ctgttcgttg ggaaacacgt gtcataataa attgctgtaa
     1741 aaagttgaat acagcagaaa tcaaatacat cacgattact gtcgcaatga tctgcgcgat
     1801 tttatcaaag tctatcggaa attttgttac ttgttggcct tgttgcatct gttgagcgcc
     1861 agccattaca cctttgaata tttctgtggt tgctttcccc aagacctttg gtgtttggat
     1921 ttggaagatc acggctgcta ttgctagtac aaatacagcg atgatcgcaa ccatacgttt
     1981 agacatatag ccaaacaatc ggcgtaccgt tccccagaaa tttttaggct tttcaccttt
     2041 tacaccgaga tttctgccag gtccaccatg tcctggaccg cctgctggtc catgagaggg
     2101 acgtgtgttt gttttactca tgctaagtcc tcctctctta gttgtgaatg aacgatttct
     2161 tgataagttt cgtttgttgc taatagttct tcatgtgttc ctttgccaac cagttttcct
     2221 tcatctagca caaggatcat atctgcatcc attactgtac ttactcgttg agcaactaaa
     2281 acggtgatac tttctttcat attcgttttc aatgcttggc gaagattggc atccgttttg
     2341 aaatccaacg ctgagaagga atcatcaaac acatagatat ctgctgtttt gactaatgcc
     2401 cgtgcaattg ccagacgctg tctttgtcca ccagagaagt ttcctcctcc ttgttccaca
     2461 tggctatcta agccatcttc catctctgaa acaaaatctt tagcttgtgc gatttctaat
     2521 gctttccaaa tcatttcatc tgttgcatcc ggcttaccat actgcatatt gtctcggatc
     2581 gttccagaga acagaactgc tttttgagga acaaagccca ttaattctcg caaattatat
     2641 tgagtcatgt cactaatgtc cgttccattt atttggatcg aaccagattc gatgtcatat
     2701 aatcgaggga tcaaattgac taaggtcgtt ttacctgaac cagtcccccc tatgattgcg
     2761 acgatctcgc ccgacttggc ttggaaatca atatcttcca atgcaagttt ttctgctcca
     2821 tgatatcgat aattgacatg attgaaggcc aatgttgcgt tttttccagc aaaagaaact
     2881 gttttaggat tttcagggtc atggataccg attttttcat ccaacacttc attaatacgg
     2941 tcagctgaag cttgtgcacg aggcaccatg ataaaaatag cggaaagcat cataaagctg
     3001 atcaagatct gcatcgcata agtcataaat gcaatcagat taccgacttc caaagtcata
     3061 tctgctatgt agtgtccacc aaaccaagtg attgctacgt ttgtaccgct catgatcaat
     3121 gtcatcattg gcatcatcaa tgcgacgatc gtattaactt tgattgccgt atttgtataa
     3181 tctttattcg ctaaatcaaa acgattttct tcaaagcgtg tcttatcgaa tgctcgaatc
     3241 actctgaccc ctgtcaatcc ttctcgaaat accaggttta aacggtcagt tttcttttgc
     3301 atacttttga ataatggaac agcaaagtac atgattccgc cgatcaaaat aatcattact
     3361 gggatgacaa ctaagaagat ttttgtcaac tgatgatctt tttgataagc aagtatgctc
     3421 gcgccaatca acgtgattgg tgcattgatc atcatacgta aaaacatttg tgtaaccatt
     3481 tggatttgag tcacatcatt ggtcgttctt gtgatcaaag aggccgttcc atatttatca
     3541 aacgcatctt ttgtcagact ctctgatttt ttatagacat ccgtacgcaa ttgcttaccc
     3601 agtttctgtg attcacgagt agcaaaaaat gtatttgcga tagcagctaa gatactgatc
     3661 aaagaaaagc cgatcatcac aaaacctgta tgccaaatat aatcgacatc tccttgcgct
     3721 acccctttat caataatatt ggaggtaagt gtcggcaaat ataaatctgc cagtacttgg
     3781 atcaccatga aaactgcagc tgcaattgct gaagtcaagg agatccgttt tacgattttt
     3841 agcatctagt tcccttcttt ctatttatat actccgtttt tcagccaaga aagtttcaaa
     3901 ttgaattcac tgattgcttg ctctactgag taatcttcag atgttgaaaa taagcgatac
     3961 gaatgcgcaa ttgttgtaaa tacagtattc atcactaatt ggatcagtaa ttccaattca
     4021 tgatcatttt ttactttcag cttcgtccga tcgatcaatt caagcaactc ttttgcatct
     4081 tgtttgtgtt ctttatgtgg atcgtgaggc gttttttggc gactcgtatg aggagcgact
     4141 tttcttgagg cacggtaatc catgttcata aacaagttac gataaaaaga agcattctcg
     4201 ccttccaaaa tctcataaat cattttagaa aaataggatt cgaatccttt gaatagatca
     4261 ccatcggatt ctttcattag ttgtaacata tctttagagc tgtcacgtct cattgtctca
     4321 aagtaataaa aataaagatc ctctttatct tcaaaatact gatagaaact gcctctaggg
     4381 atatccgcta atttgacgat attagcaatc gaagcttctt ttaagggtac tctagaaaac
     4441 tcgattctcg cagcctcgat tagtcttttt tgtttttctt taggcaaatg gaaaaatgtc
     4501 tgtgtcggca tatggatcct cctttctttc aagatgacac attgtcatat gacgctgtgt
     4561 cattataaca cctcctacct gttttgcaag acatttgcat agtttttttc attatcatcc
     4621 gatacattaa aaaagtgata agaggaatcc ttccatttga attaatgaga gttgcccatt
     4681 aaaatagcca catactaaac aaaggatgag ataaatggcc caatcaatta gtgtccaaga
     4741 cttcaataaa ttgcctctcg atgctgacag catcgtttta gatacaagaa ataccttaga
     4801 ttacacaaaa aaccacttaa gccactcgct ttctatcccc gctgctatct tgccaagaca
     4861 attaaaaaat ctagatccaa agaaacttta ttatattcta agctatacag gcaggcgatc
     4921 agaagcatta gccgatcaac ttactgctaa tggttttcgt gctgtttata tcattggtgg
     4981 gatggaagct cttcagtcga gtgttgcttg aatattgctt gttagattca aaagaggctg
     5041 tgacaaaaaa tcgtgtcaca gcctctttta taccctgtct ttttttagtt atttattctt
     5101 caaccttatc aatatgactt gatagccttt tctttttctc atagttataa caatcatagc
     5161 aataaaagga gaaggtaagc atatggcatc tgttttagta gtcaaaggtc atcccctaac
     5221 tgcagaagaa tcgcgcacag ttaaggcatt gacgtctttt ttaacaagtt ataaagaaaa
     5281 tcatccagat gatgaagtga ctgttttgga actttatcgc gatgatattc cggaaatcga
     5341 tgaagaatta ttatcaggtt gggaagcatt acgtgctggt gcagaattca cttccttatc
     5401 agaaagtcaa caacaaaaaa ttgctcgttt caatgaattg actgatcagt tccttgctgc
     5461 tgataaaatc gttatcgcaa acgccttatg gaatctaaat atcccaacaa aattaaaagc
     5521 ttggttcgat accgtcaatg tagctggaaa aacgttccgc tatacagaaa acgggccaga
     5581 aggattagtg acaggtaaaa aagcactgca catccaatct aatggcggtg tatacaacgg
     5641 acaagacttt gcctctcaat atgtaaaagg tattttaaac tttgtaggca tcgatcaagt
     5701 agatcagcta ttcatcgaag gaatcgatta tgaccctgac cgtgcagatg agttgatgca
     5761 aaatgcatta gataaagctg ctgctttagg taaatcattt taaaaattga gataaatgca
     5821 tacaaaagag acagaggaaa aaatcttcaa agcagcattt gtaccttgga tattttccca
     5881 tctgtctctt tttgttattc tttctgcttt ttcaacggat tttcttcata aagagaagag
     5941 attgttgtgt accattcaaa aacatgagta atcactactt tagctgctgc ataaacagga
     6001 atccccaaga tcacaccgat gattccgaac aatttcccag aagtcagcag aactaataaa
     6061 atcgttactg gatggattgc taactgattc cctagtacta atggagagat caagcgtcct
     6121 tctattgtct gctctactgc aaaaacgatc agtactttga tgatcatgaa tggacctgct
     6181 acaatggcta aaaagatagc cggaatcatc gctaaaaatg atcctaagta agggatcagg
     6241 ttcaaaaaac ccgcaataat ccccaacgtg acagcatact ctaaaccaat gaccgcaaaa
     6301 ccaatcataa acatgattgc cacagcaaaa gccacagtta gttgtcctct tatataagaa
     6361 gaaacttgac tattcatttc actcagcaca ttcaacgtcg gtttttgcat gcgtgtcggt
     6421 aaaaatttga caaaataagg tgctaattgt ttaccgtctt tcaatagata aaataagata
     6481 aacggcatcg tcagcagagc cactaaaata gtcgctaccg cgccgacaaa acttccaaga
     6541 ctctgaactg tactcttaga tatatcttgg atcatatcgc ctgccgaatt catgaactta
     6601 tcacctgctg tttccaactg ttcgcgaaat tggttgaaca gcgggtcccg caaaatctcg
     6661 gttaatttat tatcaatcgt atctacatat tgagggaagt tactgatgaa gctcactgtt
     6721 tgttcctgaa tcttagggat gatcactacg ctcccccatg cgattagtgc aacaaccaaa
     6781 acaaacagcc caatgatact gtaaagcctt ggtacttttc gcttttcaaa gtaatcaact
     6841 accggattca tcaagtaata caagattcct gctaaaatga taggcagacc tacgattgct
     6901 aaaaattgcc agattggtgc aaacaaatat gaaacttttg taaataaaaa gacgatcagc
     6961 aatattaaca atacaacaag taaggctgtg actacttgat tatttaaaaa ccagcgccaa
     7021 aaccacgata ccggtctgtt cttcttattt tttccttcca taattcctcc tgttagttat
     7081 aaaaaatttg gcttcccaaa ccaatagcag gcagtctatg cggatgaaga tagattccat
     7141 ttgcaatttg tccttcttct ggacaagcat caaaaatcaa tgatacgtga gctactgtct
     7201 ctaaattccc attggccatc ggaccgacat aatcgatcgt atatacctga tcatcaaaag
     7261 caattgttcc gcctgctttc aaagaaaaag gttcgtcttt tgatacttct tgaatcaccg
     7321 aatattttcg taaggcagct gttgctgttt tatcgaacaa aatcagcatg ggttcttcct
     7381 tgtttattgc ttctgtccca atttctgtaa tctttgcctg aatcattgca ctctctcctt
     7441 taattgcttt caaaaacagt ataacacatt ctcattcttt agccgtgtgg aataattatg
     7501 tgatagaata aaaacgaaaa ctatttcatt acaattagga ggaatcagct catatgatcg
     7561 aaaagataat tttaggaacc tatacgcgtc gcgaaagtaa aggaatctat acgatcgacc
     7621 ttgatacaga aaaagaagaa ttagccaacc tttcattagc tgttgaagaa aatagcccta
     7681 cctatgtagc aaaaagtaaa gctggcaatc tttacacagt tactagtgtg gatggattag
     7741 gtggtgccgg tgcgtacgat aaggattttc attttttgaa tgcggtaaca gagtctggcg
     7801 ctcctttgtg ttatgtagcg gttgatgaag cacgtcaatt actatacggg gcgaactatc
     7861 ataaaggtga agtaaatgtc taccatatat taggagatgg cgaactgaaa gcagcagatt
     7921 caatattcca tcaagaagcg acaggtcctc ataaaaacca agatcatgcg catgtccact
     7981 atacggatct gactcctgac caacgattag tcgtatgtga tcttggtaca gatcgtgtgt
     8041 atacttatga tgtgtccgaa aacggcaaat tgaatcttgt aactaccttc actgcagagc
     8101 caggaacagg ccctcgccat cttgtctttc atccaaatgg acaattcgct tatttatttg
     8161 gtgaattaga cagcacagtc cgcgtgcttt cttataataa agaaacaggt gcttttgaag
     8221 aattacagaa aatatcgacc ttgccagaag aattcgacgg tgaaaatgga ggggcagcta
     8281 tccgcatttc aaatgatggc aagttccttt atgcttctaa ccgaggacat aactcgattg
     8341 ctgtattcgc agtttttgaa gatggttccc acattcaaaa caagcaaatc atctcaacag
     8401 aaggggattt tccccgcgat ttcgcactag atccaagcaa taattttgtc gtgtgtgcca
     8461 atcagaacac cgataatctg acattatatc gccgtcatcc tgaaacagga ttactggaat
     8521 tactgaaaaa agatgtctat gtaccagaat gtgtctgtgt ttattttgat cgttagtaag
     8581 aaaaggagaa tgaaatgaat cagcaagacg tattagacag actacgggaa gaactaaata
     8641 ttcctttctt tgatgggaaa atcgaagata aagagtatag cgaagaggaa tatcagaaac
     8701 tcaaagccga tttacagaac tactttgaac aatatgtaca gaatgtagaa aactaatcag
     8761 ctttccatac tatctgaaaa gaacatcagg acaagacttt tccgtctatc ctgatgttct
     8821 tttctacaaa taaaaaaccg ccgagatggt tagactcgac ggaaaaggag tatttactcg
     8881 attttgagta agtacaaggt tgttgttggt tataggtatg tcattatcat aaaggatttt
     8941 gatcactcta tcataaaggt aatattacat ttatattaga taactctagt ttttattgtt
     9001 atcaaagatt tcttttattt tttcaacaac aaattagtca ttttttggat ggcagctgtc
     9061 aattggtctg tcgcttggtc ttgttttgct tcagatgtat tgaatagatc ttcatctacc
     9121 cctgattcaa gatcgatact ttggatcatt tcttcacagc ctgctacata tgaaagataa
     9181 gctttttcaa aatttttatg aatgcccatt gcttttgctg gtggacgcaa agtactgact
     9241 ttcttaagca ttgcccgata tttttttgtt ccttcttcaa ataacaggat gatctcttca
     9301 agagtcgatt tgtccagttc acttgttttt ccttcatcaa ttgcgtttct gatcacttca
     9361 tatttcggat gcattccttc tccgattgct tctgtttcct tgacgatttg atcgatggac
     9421 tgcacataat agccaatatt cggtaacatg ttcgttttcc tactttctcc attgttgtta
     9481 ttatttattc tacttgtttt tatcctcaaa tgacaagtac ttgaaaaaat ttaatggttt
     9541 ctatacagct cgccattctt attcgtgctc aaagaaacca tctgctaaac tatattgttt
     9601 ccctgcgaca atgtcatatt ggatcaattg atattcgtcg taaatctctt gttgcgagcg
     9661 attgaattgc gcttctttat accactgtcc gttttgatag tacagttctc gttcaaacgc
     9721 cggaagttcc tgttctaatg ataaaagcag ctgataaaag ccatttgttg tctgattcgt
     9781 ctgttccatt agattcgctg caaaataaaa cggactggtg attgcatcgt gagtcgtttg
     9841 cttttctaat tctcctttac tgtcgaacat aagaaattgt gtttcatgca atgtttgttt
     9901 gtcatttttt gcttggatcg tatctgaata gatccctgga agatgatccc cccaaaaaac
     9961 aactaaagtc cttctagaaa gattcttcaa ttcttccgtg aactgcttca gtgccgtgct
    10021 gctataggaa atatcttgca gataattctc taagtcaagt gtcccacttc catctgaagt
    10081 ttttgcagga tagtctagat tatcatactt tccatcgtag ggcatatgtg tctgcatcgt
    10141 gactaaatga atgaactgag gtgttttttc ttccttgaga agtgtcatta cttctttgta
    10201 agctgactca tcagaaatat atggattatt ctctatcgta tccgtatagg tcatcgtacg
    10261 ctcgctgatg aactggtcaa atcctaatgt ctgataaaca tcctctcttt tatacatcga
    10321 agtattgtat ggatggatcg ctgtcgtctg gtatgattgt gcgtttaacg ctgaaacgat
    10381 agaaggaagc tgatccattt ttggtacaag catcgtgtaa ggggtggtga gttgcgcatt
    10441 aaataacgcc atagaaaaac cagtcaatgc ttcaaattcg atatttgctg ttccaccgcc
    10501 atagttttga gaaagcatat tgccactata tgtttgatca gctacttcat aataatctgc
    10561 caatggctca ccagatactt ctacgccgtt taatcgacta ggatctgaga aactttcact
    10621 catcacgaac acgatgttcg gcgactcttc ctctacagtt ttattttttt catcggctag
    10681 cttctggtat ttttcagtga tttctttgat cttttctttc gaatatcctt caggttcatc
    10741 cattggttct accttcagat tgtacaaaaa cccaccgata aaaccagtgt tatagtagtt
    10801 cattttctgg ctgtaaggta tccatagtgc cgttttatta tatgcttttc gcaacaaatt
    10861 atcgggattg ttgaaattac tgatataccc tagcagtcca attgccacaa gcagtgtcgt
    10921 taatcggatc aattggaatc gccttccttt tttcaggctt cgaaacatgt accagcaaaa
    10981 caatcctatc acggttcctg cggcagctaa gatcactgta aacagcatag tgccggtcat
    11041 atctttcagc aatccgattt cagtgatcat cttcaaatcg tctggataaa tcggttcttg
    11101 ccgataactc attttcatat aatttgcaaa acctaataca ccgatggcta cgacatacgt
    11161 aagggaacct aaagggacag atcctgctag tgcaatcaga aaaataagaa taatcagcaa
    11221 gacgagacag gctaagaaga atttttccgt atgccaagaa aaagcaaatt tcaacgcaag
    11281 gtctacggat agatcatttt gacaccactg caaaaataaa ttactaaaaa caacaagaaa
    11341 aaagatactg atgattgctg ctgttccttt taaaatcttt ttctgtttgg tcgaaagtct
    11401 gcttatatat cttttcaaat gcttgctcct tcatttctat caataaaaga atgttgcttc
    11461 tcttatttta gttgtacgaa attcgaaagt aaagaacgga aagaagaatt aatgaaatcc
    11521 aaatattctc tttctttatg gcctttctgg tttctttaat aagcggacct ttcgtactcc
    11581 agaaattttt tctagctgtt gtttcacttg attgagctgt tcaaaatcag tactgtctat
    11641 gtcaatcaat atgtagacat gctcattctt atggttacgc gatatatctg cgatcgctag
    11701 ctcgttctca ttcagcagag aaaatatctc tgctaatata ttggttttct tttgataaaa
    11761 tatagtgaat cgaaacggcg cttggaaaaa cattcgtgcg gatggataat tgaccgcttc
    11821 tctaaccgta ccgaacaaaa gataatcttt taaggcttca actgccaaac gtccgctgtc
    11881 tgccaaagct tcctgagtcg atccccctag atgaggcaac ataagtatac ggtcattttc
    11941 taagaactct tcttctggaa aatcagtcaa gtatttagcc agatgttgtt tttccaatgc
    12001 ttgcataaca gcatatttat caacaatttc acttcttcct acattgataa gtacagcatc
    12061 ttttttcatt ttttctatat tcgcctgatc gatcaatcct tttgtatctt ctgtcaacgg
    12121 cagcatgact acgatgtaat ccgaggtaga aagaactgtt tctaaatctg cttgccgaac
    12181 ataatccaac tgggcatcgc ggatagaata acccaagaca tccatcccta agctatagca
    12241 acttagcgct acttttgtgc ctatcgctcc aagaccaaga agaccgatgg ttttgccttg
    12301 caattctcgt cccacataag cacttctttt gttttctgcc tgttctaaaa tattaggacc
    12361 tgttagtgtt tgtaccatac gacttgcttc gattatcgga cgggaagata agagcaaaca
    12421 gcacaaaaca agctctttta ctgcattagc atttacacct ggtgtattca tgacgatcgt
    12481 cccgttttca gaagcttttt caacgttgat cgtgttcacc ccaacaccag ctcttgaaat
    12541 tgctagcaaa tctggtgtaa gccattcatc tttcactttc gtactacgaa taactattcc
    12601 ttcggctggc aaatcatcta ttattttgaa atattcccct tgtaactttt ctatctcttg
    12661 ttctggaata gcatcaatca aatgaacgtg atacataaaa ccccctcctt actaacaagt
    12721 atctcgaaat ttctgcaatt agacaactgt ttaccatttg acttcctaga agcaaaaaga
    12781 gcagttagag cagagtaggc tgagtgataa aaataaggct atgacaagtt actgccataa
    12841 gccttatttt cgagagcaaa atattgaggg tgagatatct tgataaatgg tgttccgtta
    12901 gctgacattg ataataagac taaatttcaa aaaagttgaa aagcaaattt cgtcaatctc
    12961 ttcttatttc ttaaaactaa gtggcttttt cacaacctct ttttatctaa taaccactgt
    13021 ttccaactca actaattttg cccacgtttc aatttgatct gttgttagat ttagcgagac
    13081 aacggtatga tgaccgccac ctgcctgaat ccatgaatgg acaccttcat ggaaattcgg
    13141 tttagttttc cataacacac gtgcaacagg taaattaggt gcttcagtcg ttggttcaaa
    13201 ggcttctact tcattgatca gcagtttata atgcgtaccg aaatctgcca tcgatacgac
    13261 taccccttct cctgctttcc catcaaatac aagacgcgcc ggatcttctc tatctcccat
    13321 tgataaagga gaaacaacga ttcttggttt cgtcgcagca agcgtcggat ctacttccat
    13381 catatgggat tccaagattg cttcttggcc actagctagc tcgtaagtat aatcttccat
    13441 gaatcctgta tctttccctc gagccatgat tttcagcaac cgatcaatcg ctgcagtttt
    13501 ccagtcgcct tccccagcaa aaccgtatcc ttctgccatc aaacgttgta cagctagtcc
    13561 aggtagttgt ttcattccat acaggtcttc aaaattagtc gtaaaagcgt tatagcctcc
    13621 ttcttctaag aaacggcgaa taccgatttc ctgctgagct tgtactttta catgttcctc
    13681 ccatgtcttt tcttcataat caccatagtc gaaggtatac aattccttgt actcttcaaa
    13741 taattgatcg acttcttcgt ctgatacgcg atcgattact tgtacaagat ctccgatacc
    13801 aaaataatca acagtccaac caaattgaat ctgtgcttcc actttgtctc cttctgtgac
    13861 acccacgttt ctcatgttgt caccaaaacg agccactttt attccaaaac tttcattata
    13921 ggctactgca acatccatcc aatcagcaat ctctttttgg atctcaggtc gtttccagta
    13981 gccaacgacg attttatttt gtttatttaa tcgagcattg atgaacccat attcgcggtc
    14041 accatgagcg gattgattta aattcatgaa atccatatcg atcgttttcc aaggaatgct
    14101 ttcgttatat tgcgtagcta aatgaagcaa tggtttttgt agaagctttg tcccgcggat
    14161 ccacattttt gctggtgaga aagtatgcat ccaagtgatc acacctgcta cttcttctcg
    14221 ataattgacc tctttcatga tttttgtgat cgtatcagca gtaactgcta attcttgaag
    14281 gcggattgga taaggcaatt ggccattttc attcagttca tcgaccattt cttgtgcatg
    14341 tttctttact tctcgcagtg cttcttcccc atataaatgc tgtgatccta caacaaacca
    14401 aaattctttt tctccaatat tcaacataat tagccactcc ttttatttgt tttgtccgta
    14461 atacgcattt tttccgtgtt ttcgtaaata atgtttatct aaaatccgtt gaggcagctc
    14521 ttctgaaaat gaattgagct gtctggtaaa taaattcatc ttgcatactt catctagtac
    14581 gactgcgttc attaccgcac tttgcgcatc tttcccccaa gtaaatggac cgtgtccgtg
    14641 taaaagtacc cctggaattg ctaagggatc aattttgcgt tctttgaatg tttctatgat
    14701 aacttttcct gtttcatatt cgtacccgct gtcgatttcc tgttgtgtca aaaatcgagc
    14761 acaaggtacc gatccataaa aagtatctgc atgcgtcgtc cccatcgcag gaacatccaa
    14821 tcctgcttgt gcccagatcg tcgcccatgt cgagtgagta tggacgattc ctccgatttg
    14881 aggaaatgaa cgatataaaa cagcatgagt aggcgtatcc gaagagggat tgagtttacc
    14941 ttcaatgata tgattgtcca agtccacaac gaccatgtcg tctgctgtca tctcttcata
    15001 actgactccg ctaggtttga taacgaaata tcgtgtttca ggatcaaaag cgctcacatt
    15061 tccccatgta tatttgacga gtccgtgttt aggcaagtca agatttgctt ggtatacttc
    15121 ttctttcagt tgttccagca ttttctatca ctcctttttc ttgcaattga tgaatcgctg
    15181 ttttttcaat gtcgatcccc tcacggtaac gtctcataaa tatctgatag cctgctattt
    15241 cttctgctga aggtgtgatg gtgattcctt ggtcttgtcc aaacacgtct tctgccaaga
    15301 agttttctaa agacttccca tgtcgatcca tcatataggc tgctaataac gcgattcccc
    15361 acgctccacc ttctccggct gtgtccatca ctgtaactgg tgcctccata gcagatgcta
    15421 gaatccgctg tccaacttct ggtgttttaa aaatcccccc atgccccact aaacgatcaa
    15481 tttggacatt ctcagatttc aatatatcca ttccgattct cattgcacca aaagcactgg
    15541 ctaaatggat ccgcatgaaa ttcgctaaat caaaacggct acctggtgta cgtacaaata
    15601 atggccttcc ttcatttact cctgtaatgt tttcaccgga aaaataacca taagaaagta
    15661 atcccccgca atccgtatcg ccttctagcg ctttgttgaa taataccgtg aataatttat
    15721 ctctgcttat ctccataccc aaagaattgg taaattcttc aaaaatactg acccaagcat
    15781 tgatatccga agagcagtta ttcgtatgaa ccatgcctac tagttctcct gaaggtgtag
    15841 tgaccatgtc gatttccgga tggacatttt ctagttcttt ttctaatacg atcatagcga
    15901 aggcggaagt accagccgaa atgttccctg tccgtttggc tacactgttc gtagcgacca
    15961 taccggttcc tgcatcccct tctggcggac aaaccgggat tcctggtctc aaatttcccg
    16021 tagggtcgat cagataagcc cctgattcgc ttaacttgcc agcttcttct cctgctacta
    16081 gaactttagg caaaatgtga tccaagtcaa tcgctacgtg cgctttttcg attaactgac
    16141 caaaaatatt tttcatttct tgatggtagg ttttcgttga aggatcgata ggaaacatgc
    16201 ctgatgcatc tcctacgcct agtactttct caccagtaag ctgccaatgg acataacctg
    16261 caagcgttgt caaaaaatta atttctttca catgttcttc tttattcaaa atagattgat
    16321 acaaatgagc gatactccat ctctgcggaa tattgtattg aaatttttcc gttaatattc
    16381 gttctgcttc ttcagtgatt gtatttcgcc aagtacgaaa tggtactagt aactgttcct
    16441 tgctatcaaa tgctagataa ccgtgcatca ttgcactgaa cccaatcgac ccgattgtcg
    16501 taagattctc gccatagatt tcgaatactt ctgctgccat ttttcggtaa ctgacgcgca
    16561 gtcctttcca aatatcttct aatgaatagg tccagatacc attttctagt ttgttttccc
    16621 actcataact accagcggca ataggagtaa aagatgaatc aatcaataca gctttgatcc
    16681 tagttgatcc aaactcaatc cctaaagatg tacgaccatc tatgacatct aacatacttt
    16741 gcgcatttac ttccttcata atgatttaac tccctccttc tcgtcaaacc tgatcaaacc
    16801 gtctttttag tagcgcttac aatgattatt gtatactttt gtacgtacat gtcaagcggt
    16861 tatttacaag aatacaataa aaaacacttg ttttgtacgt acataagtta taaaatgaaa
    16921 ttgcttacaa aatctgatca accgccttct cgaaattcta gaaggagaga atacttatga
    16981 aaaaagaaaa aaaagtttcc tcatcgttta tttatttttt tggcgcattt ggcggcattc
    17041 tatttggata tgatattggt gttatgaccg gcgccttacc ttttttacag catgattggg
    17101 gtttggcagg taaagcttct ttgataggat ggatcacgtc ttcggtcatg ttaggtgcga
    17161 tccttggcgg ctctttatct ggtcttcttt ctgataagct tggacgacga aaaatgattc
    17221 ttctttctgc acttattttt atggctggtt ctgtcctttc tgcatctgcc cctcacaatg
    17281 gttcttattt tctgattgca gcaaggattc ttctaggttt agctgttggc gctgcatctg
    17341 ccctagttcc tgcttatatg tctgaaatgg ctcctgctag actaagggga agattgtcag
    17401 ggatcaatca agtgatgatt gcttctggca tgctcctttc ctatgttgca gattatttgc
    17461 taaaagtgtt gcctgaaacg atggcttggc gcgtgatgtt agggctcgct gctgtccccg
    17521 cgctgatttt atttttcggt gttttagctc taccagagtc accgcgtttt cttatgcaat
    17581 ctggccgctt ggaagaggca aaaagagtat tgaactatat tcggacacca aaagaagctg
    17641 aacaggaatt cgaacaaatc cagctgaatg tcaaacaaga aaagacaacc ggaacttctt
    17701 ggcatacttt gtttttagaa aaatatcgtt ccttagtcat tgccggtatt ggtgtagcag
    17761 tattccagca atttcaagga gctaatgcta ttttttacta tattccgctg atcgtagaaa
    17821 aagcgactgg gcatgcagcg agcgatgcat tgatgtggcc gatcatccaa ggaatcattt
    17881 tagttgctgg ttctttgctc tttttggtta ttgcagacaa gttcaatcgg cgaactttat
    17941 tgaaaatagg cggtagcgtg atgggacttt cattcatcct accagctgtt ttaggaactg
    18001 tactggatgc tcatacaaat tcgttgttga ttctcttgtt tttatgtatt tatgtcgcat
    18061 tttattcttg cacatgggct ccactgacat gggtaatcat aggcgaaata ttcccactag
    18121 ctgtccgcgg gcgagcatct ggcctagcct cttcttttaa ttggattggt tctttcctag
    18181 ttggcttgct ttttcctgtg atgactgctt ctatgtctca agaaatcgtt ttcggcattt
    18241 ttgggatcat ctgcttttta ggggttttat ttattcaaga gatcgtacca gaaactcgtg
    18301 ggaaatcttt agaggaaatc gaacaaagtg catctaaaaa aacttatcct aaacgtattt
    18361 ctatccatca ttcttaaaga ggttgtgaca gaagtggtca gcttcaagaa ataagaagga
    18421 accgcttagt ttccagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgatgaa
    18481 tattgtgact tgtgaccagt tggtaggaga acaccgttta gtggagaggt ggtgaaagaa
    18541 acgttcagcc ttgagcgatg acaaaagctc tcacaaacat tgcgacgagt aaccgtagga
    18601 gcaataagag gcggtaaaaa cccgtctctt atttttcgtt ttctttcact gtttcccgtt
    18661 caactagtac aggttcatag agaatatttg gctgtgcctg tcctttttca atcgttgtga
    18721 cgatccaccg tgcggcatct tgtcccaact tttcttttgg atgcgtcaat gttgtcaatt
    18781 ttttattccc taaatggctt aaagaagaat cgtctgttcc tacgatcgat aggtcatctg
    18841 gaatatgata gccattttct gtcaacttat ctagcattaa actagctatt tgatcgttat
    18901 agcaaatgat gccatctaaa gcttgttctt ctttcaaccg ttgtactgtt ttttcagcga
    18961 ttgtttctcg tgtttccgtg gtgaaagtaa tgatatcttc cgtcaaaaaa cgcacattat
    19021 actgttcaca cgcgctgata aaacctttca tcctgtattt tccttgaaga tcgtcgattt
    19081 ttgtgatgaa caacagacgc tgatgtcctt tttttatcaa atattctgct gccataaaac
    19141 cgactttcac atcatctaaa caaatataag aggcatttaa ctcttcataa gcagcattga
    19201 tcatcaccat cggaatgcct tgttctctca aagcaacata aagtgctaag tttgggttat
    19261 actgattact tttcgtaggc tcaacgatca aaccatctac cccataagca atcattttct
    19321 tcaaacactc tttctccaat tcgtgatcgt tattcgtact ggctaaaagc aatgaatacc
    19381 cagattctcg taattcattt tcgattcctc ggataatcga cggaaaaata taatcagaga
    19441 gatatgtggt gatgacaccg atcgtttttg ttctgttcaa tggtgttttt tctttcttat
    19501 agctgtcatc tacatatgtg cccgaccctt tttctttacg cagatagccc tcatttacca
    19561 acacagcgat tgcttgccga atcgtatgtc tgctgacgtc atatatttct tgcagcttga
    19621 attctgaggg aatggttgta tttacttcgt attcctcacg aaggatcttt tgttttatct
    19681 cttctgcaat atcttgatat tttgccattt gccttgcccc tttttatttg tacgtacata
    19741 tatcataacg agtctgcaag gtttattcaa gcaaaaatca aggtttcttg tgctcataag
    19801 gcatacggaa caggttcacc aaattctgga aatccttgct cattccacgt aaagcattgc
    19861 gcgttcgcgt gccgattagg attgtctaac ggatcccctt tttggttctt ctctggtcga
    19921 gcatgataga tcaaaatatc aaagcacccg tcttctgaaa ccgtaaacga gttgtgacca
    19981 ggaccaaatt gcttattttt ttcagaagaa acgaatactg gctccgataa tttatgccat
    20041 gattctttta ataaaagatt cttgtgctca tcagcccaca gcaggcccat ggcatagttt
    20101 tcatctgttg cactaccaga atacgtaatg aatattttcc cattgcgagt aaggactgct
    20161 ggaccttcat ttactgaaaa accccgtttt tcccattcat actcaggtat agaaaggagg
    20221 atctgcttcc cctttaacgt ccaaggatta tccatttcgg atagataaag attagaattc
    20281 ccagtaattt caggatcttg ttgggcccac acataatata gctttccttg agaagagaaa
    20341 gttgttccgt ctaaactgaa agtttccctt ttcgttttta tttgtccctt ttctatccaa
    20401 tctgtattca ttggatctat atgctgattt tcaagtacga acatgcggtg atgatgagat
    20461 tcatcacgta tagttttatg attgcttgca gcaaagtaaa tataccattt tccattgata
    20521 taatggactt caggtgccca gatcaattca ctcatcggtc cttcttcatg agcctgccat
    20581 acaatcaggc ttgcagcatg ttctaattca ttcagtgact ttgcccttct taattcaatc
    20641 acttggtagc ctggaaccga tcctgtaaaa taataataac catctgtatg tttatatacc
    20701 caaggatctg cccgttgccg aacgatagga ttattataaa gtgtcttcat cataacaaat
    20761 ttcctcacat tttctttatt ctaattacat tccaagagta tttttctatc cctacattta
    20821 attcgtcatg tttttctatt gaaaaatagt ttatttcagg ttttattttt tctggttctt
    20881 cacgtgtatt tttgtccaac agatcgctac tggttaatgt gtagtgctct ccttgatcta
    20941 gcaaattgaa atcatgttca atcgttatct tttgttgttc atttgtgcga ttgatcaaga
    21001 aaatagtcag ctgattgccg ttatctacga tcacactgtc cacataagga acatttttta
    21061 tcttttcaca atcataaaca gggcaaatga tttgttgttt caatacttga cctttagcat
    21121 atttcgataa gtgttgcatc acataataag ttggattcct ccatgcttgt cctttagcat
    21181 ctgtcagtat taaaggtatt gtgtttacta acaatgattg acaactaatc tttacgcgat
    21241 ctgcgtgtcg taaaatagcc agccctaatg aaccaaataa taatgtatct tccatagtaa
    21301 agtaacacca atcgatggga ctaccgactt caaaatccct atgtcttttt tccggttggg
    21361 catgtacatt ccattcatca aacgctagat acattgtttt ctcacttctc aaatatcctt
    21421 ttacataatc acaagtagca acgatttctt caatttgacg atcgaatcga taagcttttg
    21481 caaggtatgt cgctgtatca tcacgctcat ttttaggagg tttagtcaag tcttcttcat
    21541 catacttatc aatatagtta tgcaaagcta aataatctac attctcataa gcttcttcta
    21601 atgcgatacg atcccattca gggtacgatg gtaaacgtgg cgtagagctt ccaactaaga
    21661 ttgtttcaat tgaatcatcc actaacttca tgacttttga ggcttcattt gctaacctac
    21721 catattcata agcactctta gcccccactt gccatggacc gtctagctca ttccctaaac
    21781 accataattt aacgccaaaa ggcttttttt gcccattttt tattcttaaa tcactccaga
    21841 aggttccttt tggaaaatta caatattcta ctaaattttt tgcttcatca attccttttg
    21901 ttcctaaatt caccgtgaat atcggtttcg cttgtacatg actgatccat ttaaaaaatt
    21961 cgtggagtcc aaaagaattt ggttctatac ctcgccatgc taaatcaatt ttttttggtc
    22021 gttgttcttt cctacctaca gtatccaccc aatcataacc agatgtaaag tttcctccag
    22081 gatagcgaat gacacctaat tctaattcct ttacataaga gaggacatct tgtctaaacc
    22141 cgtcaccatc agctgtttca tggcccggtt cataaatccc tgtatagacg acacttccca
    22201 tatgctcaac aaatgagcca aacagccgag aactgatctc tcctaccgta aaatcctctc
    22261 tgatttttac tttcacttct tcgtccaaca gctttcccta cctttcattt cctatagtca
    22321 agataataga tacaagtttc catacgccac cagcaaagat aaaaataaaa ggagggaaaa
    22381 gaaatatgag cagtacaaat cctaataaca atacgcaaca aagaatattt ctcaaaaaat
    22441 caaaaaatac tttccatgca ctttgttcaa aaatttgtaa aaatgtgata tctttgtttt
    22501 cagaaatcac atatatccag gataaaagaa acatggaata aataagtaaa actacatatc
    22561 cactaattga aagtacgatt gtctgttctt ctaataattg attaacaaga aaataaataa
    22621 tgatcgttaa cagactaagg aacaagttct taccaaaatg ttgctttatt ccttgaaaga
    22681 aaaaataaat gatatttcct gatccctcac ctttccattg cgccattaca taaaaaacag
    22741 cacatgtagc aggcacgata gtaataacag gcaaacacac gattacccag agaataccta
    22801 ataagatgta atcagaaacc acaatcaact gtccataaaa tttagtctca actattttca
    22861 tcttcctcac tctttcattc cagacatagc tgttccttga acaaaatatt tctgtgcaaa
    22921 aatataaacc actagtaatg gcagcattga aataaccgtt ccagccatta caggtccata
    22981 aaaagaaatg ttctgccctg taaataaagc taaccctgct ggtaaagttc tcatttttga
    23041 actacttgtc aatatcaatg gataaagcaa gtcattccaa ctattcatca gcgtgaaaat
    23101 ccctaatgat aaaagagtag gtttgctcaa tggcaacatg attttcaaaa aaatattaaa
    23161 ctcacttaat ccatcaatgc gtgctgcctc ttccaaagaa tctggtaaag taataaaaaa
    23221 ggaacgcatc atgaagatac caaatgcagt tgtcatacga ggaatgatca aacctgcata
    23281 agtatcaagc atgccaagca aattgacttc aataaataga ggaatcatga agacttggaa
    23341 tgggatcatc attgtcagca aaacgaaata gaacaaaact gattttcctt tgaaacgcat
    23401 acgagcaaat gcataccccg caagagaatc aaagaataca gaagtgatca ctgtccctcc
    23461 agcaaaaatc actgtatttt tgatgtaatc taataaaggg atcgttgtcc aaaccttaat
    23521 atagttatct agggtatagc tatctgcaaa tattctaggc ggaaatgaga taatgtcttt
    23581 ttcgtgttta aaagaagaaa taatcagcca caaaaaagga aacgcaataa ccaacaatat
    23641 aactagcata ctaatcgttc gaatgatttt tttaccacta actgcttttc gactattcac
    23701 agtaactgta atcgagcttt ctggtgccat acaggtttcc ctcctatttc atttgttttt
    23761 ctttccgtga catgtacaca ttagtggaga tagctatagt tgcaacaatg ataaacaaaa
    23821 ccgaagacag tgctgatgca tagcccaatt cataaggtgc tgtaaatcct cgactgtaaa
    23881 tatattgaac agctgtttct gtcttaaatt gtgggccacc agcggttgca acatatactt
    23941 ggtcaaacac ttgcatcgaa ccaatcagat tcgttaaaat acaaaaacct aaggagggaa
    24001 cgatctgagg tagcgtaatg ttaaaaaact gctgtcgatt tgttgcacca tccatttgag
    24061 cagattcata caaagaggga gagattccct gaatagcagt caagagaatc gtcatagtaa
    24121 caccaaaatt cttccaaaca gtcatgatgg ctaccgtcgg catagcaagg gttggatcgc
    24181 gaaaaaattg agggatcggc aagccgactt gtgaaaacaa gtaaggaatc gcccctacat
    24241 tcggatcaag caacatagaa aaaataatcg caattgacgt caatgaacaa ataactggaa
    24301 tgtaaaatgt cgaacgacaa aaacgattga atctggtatt tttagataat gctactgctg
    24361 ctaataaacc aacgataatt tgtgttggcg tttcgagtac ggtgaaatat aaagaatgaa
    24421 tcaacgaatt aactgctcgt tcatcactaa atatacgtgt gaaattttca aaacttacaa
    24481 aatttcttga tgtaaagaaa atattcaaat ctgtaaaact aatagcaaat gttcctaaaa
    24541 gcggtatgac agtaaataat aataaaacta aaaaagatgg agttaaaaac aaatacgcag
    24601 cacgatcagg acgattccaa tatttggtga atccgttcat atagtccacc tcatctttca
    24661 ttttgataaa atatacaatc ttggtttaaa cgaatctgaa taagatatcc gaacaatcgc
    24721 tgtgattttt gcaataacac ctatctgcaa atactaggtc gatcattcag atatctgtat
    24781 cttgccaaca gtctatcctg tataaagttc tgtactattt ttgcagtaat tgattgatgg
    24841 catcatctgc atcgttcatg agtgtctcag gatcttctcc tgctaataat ttttcaatca
    24901 gtggattgat gatatcgtta ttgatcgcag agatttgggt aaaacctggt aaatatggct
    24961 ctgcatagtc catttgtttc gataattcag atacaatagg atcatttttt acttcttcat
    25021 cttttgctac tgaatccaag taggcaggaa aaccattttc gatactccat tttttaccaa
    25081 tttcagttgt attccaatat ttcaaaaact catagacagc ttctttttta ttattatctg
    25141 tacttgccgg aatggcatat cctacgccat ctaaaatagc tgtttttttg tctgaatcac
    25201 tcccttgtgg taaggtagtc acattataat caatctcgtt tgctttcaat ccgttattta
    25261 accatggccc attgatttca atggctagtt gaccagcatt cattaaattg tccatttcgg
    25321 cgccagaaat cgattctgga ccagtcttgt ctacatggat cattttttgg atcgctttta
    25381 acgttttaag gttctcagga gacgcaaatt cagacttagt ataatcatcg ttcactaatt
    25441 taccgccatt tgctaacatc cagttatata acggtgcagt tccatcctta ttgaagacaa
    25501 aacctgatac atttttacta ctatcagtta attttggtgc catttctgtc aattcttccc
    25561 aagtagtagg tggttgttca gtatctaatc cagcttgttt aaacaaatca ttattccaat
    25621 acatatacat gccttgtacc tgcatgggga caaaatactg tttaccttct acttcaccta
    25681 attcaatagc cgtttcttta aaatcagatt tatctactcc atcatattcc cagaaatcgt
    25741 ctaatggttg taccgctccg ttttctacat actgtgcaaa atctggataa tttaatgcaa
    25801 taaagtcagg tgctttttta gaagaaatcg ctgtaggaag tttttcattc aaattggccc
    25861 aagtcatgat atccatttca attttgatgt ttttatcatt tgttttatta aattcattaa
    25921 cgatttcttg caatatttca ccatcagatg cagtaaaccc attccaaaaa gtcaaagtca
    25981 cttctttatc agaatcagat tctgctgaac ttccgccacc gcaagctcct aaaatacctg
    26041 ttgcagctac caacaaagag ccaattaacc attttttacc tttcattttt tgttcctcct
    26101 tcatctgata aacgacatct gaccacattc caagatagcg gtttcaataa acaattgatt
    26161 ttcccatctt cagcttttac atttgttgaa tgacttggtt ttacttgttc acttttagga
    26221 agattagttg cttttgtatc aaatccactc atttcagaaa aatcaatcac tccctctaat
    26281 gataattcag acaagtgaac agttagattc atttcttctt ctccacggtt aactgcaaat
    26341 atgacaagtt ctttgtcatt tattacggct atactttcta aatacggcac ttcttcaaaa
    26401 tcttccgtcg catagtttgc tgaacgaatt tgtgggctta acacctctcc ttgtccatac
    26461 acagcagctt gcataaatgg ataatagatc gtctgtctcc aagcttctcc gtttgtttct
    26521 gtcctgattg gtgcaataac gtttaccaat tgagcaagac aagcaatctt tactctatct
    26581 gcatgtttca ataaagtgat taatagacag cctaatagta atgcatcttc aaaattatag
    26641 ttatcttcta gtaaaggagg agcaatctgc caaggcgctg cttgctcatc ttgttccgct
    26701 gaatgatacc aaatattcca ttcatcaaag gacaggttga tctgcttctt actatgtttc
    26761 ttcgccttga ctgtatcgca catcgctgtc acttcagaaa tgaaccgatc catgtctaag
    26821 gaacgtgcaa gataatgaga aagatcattt ttttggttgc cataatattg atgcagagac
    26881 aaataatcta tttgatcgta cgcttgatca agaactgtta actcccaatc accaaatgta
    26941 ggcatttgcc gattcgaact tccgcataaa accacctcaa ttgaatcatc tacgagtttc
    27001 atcgccttag ccgtttcctc tgctaaccgt ccatattctt ccgctgtttt atgtccaatc
    27061 tgccaaggac cgtccatttc attccctaaa caccatgtct taattccaaa agggctctct
    27121 tgaccattcg aacgccttaa atcactccaa taagttcctt taggaaaatt acaatattcg
    27181 actaaattac gagctgcatc tactccacga gtacccagat tgacagccat attgatttcg
    27241 gcccccacat gctgacacca gttataaaat tcatgaatcc caacttcatt cgtttcaatt
    27301 gtccgccaag ctaaatccaa ccttctaggt ctatttgcta tgggcccaat actgtcttcc
    27361 caacgaaatc ctgacacaaa atttccacca ggatatctaa tcatgggaat gttcaattct
    27421 tttaccaagt gaatgacgtc ttggcgaaat ccacttttat tagctgatgg atgatctggc
    27481 tgataaatcc cttcatatac tgctcgtccc atatgttcaa taaatgaact gtacaaccgc
    27541 ggatcaactt tgcctacacg cttgacacta ctaatcaaca ttgtggcttc cataactgac
    27601 ctcctttaat ttatacatga ataatacacc tcgttgaaag cgttgtcaat aatataaatg
    27661 ttaaac
//
LOCUS       DAFFSV010000024        27263 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFFSV010000024 DAFFSV010000000
VERSION     DAFFSV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.024"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..1835)
                     /locus_tag="LR110_000937"
     CDS             complement(<1..1835)
                     /locus_tag="LR110_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4821937.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="LR110_000938"
     CDS             complement(1832..2536)
                     /locus_tag="LR110_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4821938.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="LR110_000939"
     CDS             complement(2548..4857)
                     /locus_tag="LR110_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL4821939.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="LR110_000940"
     CDS             complement(5052..5516)
                     /locus_tag="LR110_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821940.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="LR110_000941"
     CDS             complement(5516..6142)
                     /locus_tag="LR110_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4821941.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="LR110_000942"
     CDS             complement(6149..6514)
                     /locus_tag="LR110_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL4821942.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="LR110_000943"
     CDS             complement(6504..6842)
                     /locus_tag="LR110_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821943.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="LR110_000944"
     CDS             complement(6832..7158)
                     /locus_tag="LR110_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL4821944.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="LR110_000945"
     CDS             complement(7136..7417)
                     /locus_tag="LR110_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBL4821945.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="LR110_000946"
     CDS             complement(7419..8783)
                     /locus_tag="LR110_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL4821946.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="LR110_000947"
     CDS             complement(8796..9365)
                     /locus_tag="LR110_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBL4821947.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="LR110_000948"
     CDS             complement(9337..10524)
                     /locus_tag="LR110_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL4821948.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="LR110_000949"
     CDS             complement(10588..12315)
                     /locus_tag="LR110_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL4821949.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="LR110_000950"
     CDS             complement(12312..12764)
                     /locus_tag="LR110_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBL4821950.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="LR110_000951"
     CDS             complement(12876..13256)
                     /locus_tag="LR110_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL4821951.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="LR110_000952"
     CDS             complement(13253..13639)
                     /locus_tag="LR110_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821952.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="LR110_000953"
     CDS             complement(13641..13919)
                     /locus_tag="LR110_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821953.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="LR110_000954"
     CDS             13967..14695
                     /locus_tag="LR110_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBL4821954.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="LR110_000955"
     CDS             complement(14841..15317)
                     /locus_tag="LR110_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBL4821955.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15624)
                     /locus_tag="LR110_000956"
     CDS             complement(15277..15624)
                     /locus_tag="LR110_000956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821956.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="LR110_000957"
     CDS             complement(15611..15796)
                     /locus_tag="LR110_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821957.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="LR110_000958"
     CDS             complement(15797..16069)
                     /locus_tag="LR110_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBL4821958.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16269)
                     /locus_tag="LR110_000959"
     CDS             complement(16066..16269)
                     /locus_tag="LR110_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821959.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="LR110_000960"
     CDS             16394..16606
                     /locus_tag="LR110_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821960.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="LR110_000961"
     CDS             complement(16639..16833)
                     /locus_tag="LR110_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821961.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16841..17029)
                     /locus_tag="LR110_000962"
     CDS             complement(16841..17029)
                     /locus_tag="LR110_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821962.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            complement(17026..17166)
                     /locus_tag="LR110_000963"
     CDS             complement(17026..17166)
                     /locus_tag="LR110_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821963.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17656..18021)
                     /locus_tag="LR110_000964"
     CDS             complement(17656..18021)
                     /locus_tag="LR110_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821964.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18061..18363)
                     /locus_tag="LR110_000965"
     CDS             complement(18061..18363)
                     /locus_tag="LR110_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL4821965.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18363..18662)
                     /locus_tag="LR110_000966"
     CDS             complement(18363..18662)
                     /locus_tag="LR110_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821966.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18655..18819)
                     /locus_tag="LR110_000967"
     CDS             complement(18655..18819)
                     /locus_tag="LR110_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821967.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18825..19094)
                     /locus_tag="LR110_000968"
     CDS             complement(18825..19094)
                     /locus_tag="LR110_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821968.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19106..19855)
                     /locus_tag="LR110_000969"
     CDS             complement(19106..19855)
                     /locus_tag="LR110_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4821969.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19858..20544)
                     /locus_tag="LR110_000970"
     CDS             complement(19858..20544)
                     /locus_tag="LR110_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821970.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(20550..21221)
                     /locus_tag="LR110_000971"
     CDS             complement(20550..21221)
                     /locus_tag="LR110_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBL4821971.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21214..21555)
                     /locus_tag="LR110_000972"
     CDS             complement(21214..21555)
                     /locus_tag="LR110_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821972.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21733..22431)
                     /locus_tag="LR110_000973"
     CDS             complement(21733..22431)
                     /locus_tag="LR110_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBL4821973.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22518..22988
                     /locus_tag="LR110_000974"
     CDS             22518..22988
                     /locus_tag="LR110_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821974.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23031..23210)
                     /locus_tag="LR110_000975"
     CDS             complement(23031..23210)
                     /locus_tag="LR110_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821975.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23197..23535)
                     /locus_tag="LR110_000976"
     CDS             complement(23197..23535)
                     /locus_tag="LR110_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBL4821976.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(23551..23754)
                     /locus_tag="LR110_000977"
     CDS             complement(23551..23754)
                     /locus_tag="LR110_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4821977.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(23769..24545)
                     /locus_tag="LR110_000978"
     CDS             complement(23769..24545)
                     /locus_tag="LR110_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL4821978.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            24844..25164
                     /locus_tag="LR110_000979"
     CDS             24844..25164
                     /locus_tag="LR110_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4821979.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            25182..25613
                     /locus_tag="LR110_000980"
     CDS             25182..25613
                     /locus_tag="LR110_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL4821980.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            25743..26204
                     /locus_tag="LR110_000981"
     CDS             25743..26204
                     /locus_tag="LR110_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBL4821981.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            26238..26873
                     /locus_tag="LR110_000982"
     CDS             26238..26873
                     /locus_tag="LR110_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBL4821982.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            26986..>27263
                     /locus_tag="LR110_000983"
     CDS             26986..>27263
                     /locus_tag="LR110_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821983.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKS"
BASE COUNT         7854 a         5524 c         4252 g         9633 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattatcttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa tttttttctg
    16861 ctagatggag caaaagcaat ccgacatcaa gaatcttttg acccattatt tcagctactt
    16921 tgctcggctt aactccttga gaaaagagca agcgtgcttg ctctatctct tcgtgagtcc
    16981 aaataaattt ggcttcctca aggataatta ctggatcagt tgctctcatt gttcctctgg
    17041 aaaaatgatg tcattcttat gcttggacca ggtatctgca tatggaacaa agtattgtct
    17101 agctaattcg atttgatcaa ttaaagcagt ttctgataat tcatggtctg cagcaatttc
    17161 attcatacta tcgccttcat ctaaacagat aagaccgttg acgttatcga tttttacatt
    17221 tgtgactgct tgactttctc cacgctcata atattctaca gttgatactg atacatcagc
    17281 ttcggagttt aattgcagaa tctgttactg ccgggataat ttaatattct tttcataatt
    17341 cacctttttt cctactgaca tcgaatattt ttcatgttat gattagccgt gaaaggagag
    17401 ctatattatg tgtttacaca tgatgtcctt agctcattcg gttctttgga agcttttttg
    17461 gaaataactc tcaggttctt gacgcaaatc tcatttattc tttgaaacaa aaaacttcca
    17521 ttttccgaga gctttggatc acttcaaaag agatttgcat ttatatttct gtgaccttga
    17581 gtcgcgtaaa gaaccttaca gcaatggcgg caattccact ttcaggaaac cgcctttttt
    17641 cattgatatt ggtggttagc ggactaccca ttttctttcg gcagtgccaa accacaaatg
    17701 atctgtgttg atgtttaggt agttgtatac cagataagct aaatccaaag tgcctgcatc
    17761 ttcaagacga caatattcca aaaattctcc catagctgga cataaaaatc ctggagctat
    17821 catatcccaa cagttaatag tgcaaagaaa ttcgtttagc ttctgaacgt tttctttggt
    17881 ttcacgaatt acaacatact ccaattcttc tccgctggca gagcattcca aaacttcaaa
    17941 ttgattaaga aatggtagaa catatgcttc aaacatcaac attccatctt tctgtttttg
    18001 cacttgtttt gcatcaatca tgtctatcct cctattcagt tatttcttcc attaatggaa
    18061 ttatttcagg tcactggact taacgaaaac accattgacc atctttccag tccgaccttt
    18121 gatttcgtca taagcaaaat ccaagcactc gtataaatcc atatcatttt gcattgccaa
    18181 gatgatcaag gttactacta catcgccaat tccgtctctt aaaccatgtt catcttttct
    18241 agctagagaa gcggcaactt ccccgatttc ttcgaccgtt tttaacattt gcttgctgaa
    18301 atccgcttga tccaatccct tatcttttgc ccactgctct actttcgcga ttagttcgtc
    18361 cattatttct cctccacata cctaaactgt cttccttttg aatcaatcca taagctccta
    18421 gctctatccc aaataatatt tttgctcaga ccagtaattt cagataactg tttagcggta
    18481 cccgttacta gaattcgatc accatgccag attgcaatct ttctcggcgt tctccgtttg
    18541 ggcttttcag tccacattga tttaccgagc ttttggactt ctgcaactat ttttttatct
    18601 tcctgccaat cttctgaata agtcaattcg ataattcgct gcattgccgc tttcttatcc
    18661 atcccgacat tctcctttca gtaatttgag tacttgatca agtgcgctct cacgtccgcc
    18721 atgaaatgtg ttgagccact tgtcttcgta cgacacactt tgtcttaaag cttcttgatg
    18781 cattaattcg atctgtgctg taaatgtctt taggtccatc ttgtttacac ctgctcaagt
    18841 tcattaagat gtttttgcaa gcctttaaca caatcaatga atagtaattt ggtatatgct
    18901 aaatttctta attgcgttcc atcgatatag agtgcgaaat agtatctgag tttactccaa
    18961 ctcgaacgat cattcttaat tccttcaatt ccagcttctt cgagttgatc atatacgtct
    19021 ctcaggacgt ctaattcctc gcccttttta taattcgcta tttcattaat tagttctaga
    19081 taatcgatct tcatttttcc acctcttaga atggtgcttt tgattgtcta ttagctcgtt
    19141 ctagcgcttt tttcttgaga taggcttctt ggtcgattgc ccattcagga agcttctctc
    19201 gtcttcctgt gcgcttgtat ccactgcttg cgttcttagg ttcacttttt tctttccttg
    19261 cccaacttcg aatagttgcc aaatagtttt tataagtctt accagatgat tcacaatact
    19321 ctgacagtcg ttcgattcgc tcttggtaat cattagggaa ttctgttttg agtttctcca
    19381 tctgctcatc tgacaaaaga acatttttat actctccgta tttatgacgg acgggcttag
    19441 ccttcgattt tttcgaaggc ggtaactctc ttatatattc ttttgtatta ttaaatgtat
    19501 tattaataga tgtattatta tctttgactt tttcgtcaat aggggtattg cgtttttcgt
    19561 caatagggta ttgattaatt cgtaggtacc tattgattat ttgattggta ccctctttgt
    19621 aaatgatttc ccgattcaag tatccaaact taatcaaatc acttacccat cgcgatatgg
    19681 tctctttatt cacaccatat aaatctgcaa agtactcatt gcctgcccaa caaaagcctc
    19741 tttcattaca caaggccgtt atctctccgt ataacaactt agtatttggt ttaagtcttt
    19801 tgtcgtacct tacgttggct ggtataatcg cataataact tcgatgttct gtcattttta
    19861 ccctccaata tttaactttt tgattgtttt ctggtttaat ttgatccctt tgatttgata
    19921 tttatttttg aaattaatca cacctatttt gtgcttctct gtgtgatgga ttctgcagag
    19981 tgctgcaaat gtgtactctg catgatcaac ttctttgcgc tttcgtcttc ctagcgcttt
    20041 gtcaaagtga tcgatgtcag ctcctgtttt gccacagatg cagcagactc tttttgtgat
    20101 gcatttgtag aagtaatatt cttgattcgc aggtaaaatc tcatagcctt ctttgaaagg
    20161 aatatgatgt tcaaagatga aatctaagat gatatttgct aagacattgg catcactcac
    20221 agttgtattc gattcgtctt tcaggcttat tttgcgccct gtgacacctt caaaacgaaa
    20281 gtagaagaat tccttccaga agtccgttgg catgcctgta tcgataaaaa tatcgcctat
    20341 cagcgcatag atgaagtttc gttgctgtac ggtgaaacgt ctaggatcaa taaaacgaat
    20401 ttcaatgact cgatcaccat cgtagccgtc atacatcgtc tttagtcgat caatgttcac
    20461 ttcctcattg atggttgcgc ttatgtcttt tcctttgaac tttttcagaa ccgctgaata
    20521 tgaatcgatt aatggtttaa acactcatat cacttcttat ctaattcttt tctcttagct
    20581 gctattgctc gctccatcaa ggcacattgc tcatagctta actgttcaat agtttcaacg
    20641 ttatcagcta agagccctaa tttatctgtc tgctcattaa catattcgat taaggttttg
    20701 gtcatatctt tacccatctg ctcattgaaa gcttctagaa tcgtctctag catgtttaat
    20761 ttctttgtat cgattctagg tggtgttgga atatcttccc cttgaaatac atataatccc
    20821 agtccgtgta gagccaatgc tttcacaaag catcgcttca atgagttatt gatttgcatt
    20881 gcatttggtt taacaactgg ttggtttcga taatctaaaa caggaaataa ttcggtttcc
    20941 gtgtgtcctt taaccgttac tgagacagat acataagtcc cagtttcatc cataagaaaa
    21001 ggtttatatt cctcaacaag aaagtcttga tgagttccag aaacaaccct gtagtgtttg
    21061 tactcattaa tagttaccgt tgcctgtgga tcattctttt tcataatctc ccacgcgtga
    21121 gcccaagata aataatcaaa atttcctttt ttcttgagaa ttttatttaa cttacgacta
    21181 aaaagttttt caaagttcgt tgtccctttg atttcactca tcaaattctg cctccatttc
    21241 agcaatgtat ttcttacctg gtccgtaata agagatatca atcaagttat ctctgtcgta
    21301 ctcttctagc gcatcaatca agccatcttc gatgacgtaa atatattcag gtttattcga
    21361 atgcttcgat agatggataa gataaacatg atcccaaata gttacatggt tgcctaaatc
    21421 atcttgatcc caagctagtt cttcattcgt caaaagattt cgtctgattt ttcgaccact
    21481 tgtttcctca attttcggct tgccccaatc aggatcaatc aaatattgat ctagagtgga
    21541 aagttctttt tccatatgct aaaatctcct tatgatgtgt tttctttgtg actctttgct
    21601 tgccggcgga gtcacttttt tatttgttgc catgcttttt gcttgtcaat atgttgttgg
    21661 cttaggatga ttggtttata gtatttccac cagcaattag caattgccgt ccctattctt
    21721 agcgcttcag ctctattcat tgtcatcacc aaaaagtctc tgttgtctgt tcagttgatc
    21781 aatttccata cggatcgcag tttctggcaa ccacatttca ataaatgaaa cagcatcatc
    21841 gaatctctta cgaggtaact cgccatatct tgggattgaa aaggtacgtt taaattcaga
    21901 ccaaaatttt gagaatactt ttttgctgat ttcttcataa gctcggcttt cttttccccc
    21961 tagaactccc ataactttca tatttccttt ttgcttaatt tcaaactctt gttgtccgct
    22021 aattcgcata gtatctttaa gcatggaaac atctttttta acatctttca tttcttctaa
    22081 ttgatagatc atcatatctt caattgtttg aggaacagta ttcttgcgaa taacatcttc
    22141 catttcgttg aatgcttcaa tgtatttttg tttgaagtag atagctttct ttcctgtaaa
    22201 ccccatagcc agcaagaaaa atccatctct actaatgaag aaaactcgtc gatttctgcc
    22261 atatgaatct ggttcattac cttctacaaa catctgtcca aaattggaca catctttttt
    22321 tagtgcatca atatctctta aaacatgttg atgttttttc tcgaagcttt ctgccacttg
    22381 taagctcgta gtcacagctt ctttattttt caaaattact aattcttgca ttatttcttc
    22441 tctccttttt ggtataattt agttaaaaac tggtggtgtt tattttgaat ttttttcact
    22501 taacaatttt tcaatggatg tcaataataa gttttatatt gagtacgttt ttaacaggga
    22561 taaaaataaa aggaatgcgc cctcaactag aagttgaact taatgcttct tacttcgctg
    22621 cagatatgat ttatacaaaa gtaatcattt ctaactattc aacagaacca gctatgttag
    22681 ttaatttaga actttcatcc gctaacttaa accgcagatg gtcagctact ccatttaaga
    22741 aattaattgc taaaggagga tcagtcgatg ataatagaat atactctgaa gctgtaccgc
    22801 taaatattcc tcctaaaagt gctatatctt gttaccttgc atttgaagta gggaaaatta
    22861 attttaataa actccttaat caccaaacaa aaatgatatt tactctaaat agaacccaaa
    22921 tccacaaaat tgttgatatc aaaaatacaa acttcccaat agagaaatta gtgaaagaat
    22981 taaactgagt aatagtaata tacattctag tttctctttt ctattcattt ttagtcagcc
    23041 ccctcggttg gctttttcgc tctgtactca gcttcagctt catcaagccc cataaaaatc
    23101 caaaccatgt aaacaatcgt tcctatcaac gcttgcctgc ttccccaaag tcctaaagcg
    23161 tagatgatta gcggtgcgct gaacactagc gctctattaa attttcccat ccgcttgcct
    23221 ccttaagatt tccgaaaaat ttgtttctaa aaattcaagt gttttacttc ttaaaaatag
    23281 atatgtgtct cttccttcaa ctggataata gacaaatcca tttttgtttt tctcgatatc
    23341 gataatattt ctatatcttg ggttctttaa aactctagaa gtaaaccaat catattttct
    23401 gttaatccgt tctagcactt ctggcaacgt catccatcta ccagtatcat cagctttttt
    23461 taactcctca taatccactt gggagataat tacatagcct tctggaattg ggatttttgc
    23521 ttctagatat tgcattagct gttcctccct ttacaattcg tacatagtga taatcgaatc
    23581 tataattctg tttgcttctg cagaagtctt tttaccgttt aaaattaaag ataagtagct
    23641 tttgctaatt ccaaatcttt cagcaagcat ggtgtaagtt aagaactttg aactttcgac
    23701 atattctttg attttttctc tatctcgttg agtgatttct gcaatgtcag tcatactaaa
    23761 actcctttct aaccaatttc ctctaaatcc atttgagggt aatatccttc tttttttagt
    23821 aattcgtaga taaatagacg ccctttctgt gtccatttgg tattcattac aattttagtg
    23881 ccaccatcgg ctttcgggat ctcagttgta tgagattttg tgtatccttg gttcatgtgt
    23941 tttttgcaca ataaccattg gttaccgact tttttctgaa tacctagttt atgaagtaat
    24001 ttattcatct gttgtggaga catcccataa tctgctgcaa tctgactaat tgttactgaa
    24061 tctgtagaag ataatatgct atctaaatag gagattttag gttcgtactc tgcaatcttt
    24121 tgttctgcga ttaatcttcc agttctttct tcttttagtt gagttgctaa ttgaatgatt
    24181 gtatctggat taagcaaagc ttcttctact ttttctggag ttagataacc tccatgtttt
    24241 ctaattgctg gcaacacttc acttgttacc caacgtttaa attttttggc agagggaagt
    24301 tttgatttta agattaaact gtacaaacca gattcgttga tgattgtcat ctctcttgat
    24361 tgacctgagg tcgtgattcg cgaccccatc ttatcttcca aatctacatg ccgcgacaaa
    24421 gcatctttag tgtttgaata acccaaaaca ctcgcaacgt ctttgcctac aaaatatggt
    24481 tcatcattta ctaaaattgt ccgaacttcg ttttgttcga aattaaaaat ttgcggtgtg
    24541 ttcatatttc tcattccttt cttttatatt cgtaaacaaa tttaacaact tttttctaaa
    24601 ttcaattgac agtatctgag taatactcta tactaagagc ataattaaat aagacataaa
    24661 aacattgatt ttaaagcttt cttggcggtc tgcatttaca tatcaatagt gttttttgtt
    24721 gtctttttag ttgttaaatc tgtttacaag atatagtata gctcattact cagaattagt
    24781 caaattattt ctgcgtattt ttctaaactt tttttgtaaa catttagaaa ggttgtttta
    24841 tcgatgaata cctatgaaat aataaaagac ttggcgaaga ggaaaaagat atctatacgg
    24901 cagttagaaa ttaattttgg atattctaac ggttatctag caaagtggaa aaataatact
    24961 cctaattcag atgaattaag tcggattgct gactatttcg atgtctctgt agactacctt
    25021 ctaggtagag aagaaagaga aacccctaaa catgtggatt tatcagaaga cgatactgtt
    25081 ttttcttttg atggaaaaga aatatctaag gagacaatgc gtaaagcgat tgcaattgct
    25141 aaagctttag aggaaaatga atagttggag tgatggtttg tatgtatttg aaattgaaag
    25201 aaatgttgag cgaatacaat ttgaaagtag tttatatgga gatgaaagag cctggtttct
    25261 actatccaaa acccagaatc atttttttga atgaaaatct atacggtgaa actgcagaag
    25321 cctttcattt atctcatgaa cttgcacatt tcagcgcctc tcattttgaa ttttcagttt
    25381 tgtacgatac ttctacaact tttcactcaa aatttgaaac agaagcagat aaaatagcta
    25441 ttctaatttt attaaatatc tatattgaga acgaactgac tgatgaatct cagtttaatc
    25501 tcgaaaaatt tatggagtat tattctattc agaataagct cagatacact tgttatgctg
    25561 tctgccaatg ctattttaag aaaaaatatt cttacgcaag gcaatatgta tgaatacatc
    25621 aagaattatt aaaaaaaccg atttcagcaa aacattaaaa aagcccgtgc tgcaacacgg
    25681 gctctttcct catatatgag cttctacaaa aaaaatcata tcacagaaat gaggaagaaa
    25741 aaatggaaaa agagatttta ataaaatctc caggaaaaac cttgattaaa gtaactgatg
    25801 attctatttc aattattaga aaaggattta taaatttagt taatcaaggt attaaaggag
    25861 aaaaaactat cccctttaaa aatatatcag ccgtacaatt aaaaaaacca ggtatgagta
    25921 atggttatat acaatttacc ttgctaggcg gaaacgaaag tcgcggaggt atattagcgg
    25981 ccacgaaaga tgaaaacacc gttatgttta caaaaaaata ttggaatgaa atggaaaatt
    26041 taaaaaaata tatagagaag caacaatcta tattagataa taattccaaa gaaacacaaa
    26101 tttcaagtgc agatgaaatt aaaaagtata aagagttgtt agatgaaggg attattaatc
    26161 aagaagaatt cgattttaag aaaaaagaat tgcttggttt ataatattca atatcaaaag
    26221 agaaagagga tctattaatg gacggtatat ttggttttac tggtattgtt ttatttatta
    26281 taggtttaat catgttaatt gtaagattta tcaaaaaaac aaataagaaa acacctatga
    26341 ttctccttat tatcgggatt atttttacta ccgtaggttt ctcgctttcc tcatcaaacg
    26401 aggcaaatga caataacaaa caggaaagct catcatctga tactcaaact actacatcta
    26461 acagcagcaa taaaagccta actcaatttg aagaattttt agagacaaat aatcatgact
    26521 ggggtgcttt cttagatagt tattattcta ttactccagc tactgagcaa aatacagctt
    26581 ttactaaata tattagtggt aaaacatata cttttgaagg aactgttatc gaatcaatga
    26641 ctactagaat agccattata gcagacaaag agtacgatga taaaagttgg aatgatattt
    26701 ctgcaacacc taaagtttca tatgtaattt ttgcgaagga cttaaacaat gctgatacct
    26761 ttacaaaggg cgataaagta acttttacag gagaaattag ttcaagagga tcgaacatag
    26821 aaaaatccta tgctcaatgg gatatgatta atagtaaagt aagtaaaaaa taaatgaacc
    26881 acgccccact aagagttaga ttgtgcacaa taataaaacc tatacaaaag agtgccatca
    26941 ggagtcacaa tgcttgtaaa ggaaatgaca aaataggagg taactatgga taatagcaat
    27001 gaaaaactaa ccaaaaaaga atatcttgaa ttaatagttg aaggcggtat tggagcaatt
    27061 ccttatattg gtggcgttgt gcagtcactt tattttgggt ctaaaaacga gaagcgtttc
    27121 aaacgcatcg agaaattcta caaaaattta aacgaatcat tagaaaacat tcgcgatcaa
    27181 attccagaag atgttttcaa cttagagaac aaggaccaac tcattggaat attcgaaggg
    27241 ataaatgacg aggtagaaaa atc
//
LOCUS       DAFFSV010000025        26551 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFFSV010000025 DAFFSV010000000
VERSION     DAFFSV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.025"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(19..729)
                     /locus_tag="LR110_000984"
     CDS             complement(19..729)
                     /locus_tag="LR110_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBL4821984.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="LR110_000985"
     CDS             complement(726..2198)
                     /locus_tag="LR110_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL4821985.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="LR110_000986"
     CDS             2336..2809
                     /locus_tag="LR110_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL4821986.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="LR110_000987"
     CDS             complement(2962..3513)
                     /locus_tag="LR110_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL4821987.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="LR110_000988"
     CDS             3618..4148
                     /locus_tag="LR110_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBL4821988.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="LR110_000989"
     CDS             complement(4631..5074)
                     /locus_tag="LR110_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4821989.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="LR110_000990"
     CDS             complement(5090..6250)
                     /locus_tag="LR110_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL4821990.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNKSVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="LR110_000991"
     CDS             6757..8457
                     /locus_tag="LR110_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4821991.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="LR110_000992"
     CDS             complement(8559..9392)
                     /locus_tag="LR110_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBL4821992.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="LR110_000993"
     CDS             complement(9696..11861)
                     /locus_tag="LR110_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4821993.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="LR110_000994"
     CDS             12121..14415
                     /locus_tag="LR110_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL4821994.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLSQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="LR110_000995"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="LR110_000995"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL4821995.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="LR110_000996"
     CDS             15066..16073
                     /locus_tag="LR110_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL4821996.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="LR110_000997"
     CDS             16095..17114
                     /locus_tag="LR110_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4821997.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="LR110_000998"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="LR110_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL4821998.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18872..20293)
                     /locus_tag="LR110_000999"
     CDS             complement(18872..20293)
                     /locus_tag="LR110_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4821999.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20536..22413)
                     /gene="tdc"
                     /locus_tag="LR110_001000"
     CDS             complement(20536..22413)
                     /gene="tdc"
                     /locus_tag="LR110_001000"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL4822000.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22704..23960)
                     /locus_tag="LR110_001001"
     CDS             complement(22704..23960)
                     /locus_tag="LR110_001001"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBL4822001.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24072..24277)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24540..25841
                     /locus_tag="LR110_001002"
     CDS             24540..25841
                     /locus_tag="LR110_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL4822002.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25910..26509)
                     /locus_tag="LR110_001003"
     CDS             complement(25910..26509)
                     /locus_tag="LR110_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4822003.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         8057 a         5474 c         4755 g         8265 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaag
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggttggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgatttgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaacagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcaga aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgagat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttacctgac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgtcg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagatc
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtac ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgccgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tatctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt tttatttttc ttctaaactt ttttctacct
    18901 caatccccca agaatctttc ttgaagtgat tgatgatcaa tggaataacg aacatgacca
    18961 ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc tgtacctaat gtagcaaata
    19021 cgacaacgat gatagagaag atcagcatgc agctgactac ccaagctaat ccgtttccgt
    19081 ttttaccaat tcggtaagga cgttcgaatt ctggatgttt cttacgcatt ttaagcaatg
    19141 aaacagcaat caaaatgtac acgatacaat aaataacagt tgtagcattt gtaagtgtca
    19201 agaatacagc acttacgtca tccattacac cataaagcaa agcaaataat gaaataacga
    19261 ttgattgtgt tagaacgaca tttctagata cgccgtattt gttttcttta tggaatccga
    19321 attttggtgg aaggaatcct tcacgagcta cttggatgat tgtttttgat ggtccagtaa
    19381 cccaagctga taattgtaaa agcacaccaa taaataccat gaagctgaag atatttccga
    19441 tcacttctgg taagcctagg atttgacagt caatcaagat aggctgcgtg atatttgcta
    19501 attccatttt tccatctggt acgacatttg ctacaagcat cgcattgatg acattcaata
    19561 aaactaatcc gatcaaagcg ataaatacgc cttttgtata attttttgtt gcgtctttca
    19621 aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa aataaatgtg ataccagcta
    19681 agtatttcaa tgaatcaata tgttccaagt cagggaaagc ttttgaccat gaaaagtcac
    19741 ctagatagcc gtttgagact agtccgactt tgaaggctgc caaaacacct aaaacgaaca
    19801 tgatgactac tgggatatat acccctagcc atacaccgat gttcccgccg acttttgcca
    19861 tatcgaattt caagttcaag atcgtgatga tccagtagat aactaaaata caacctaaaa
    19921 cgaaccagtg attattgcca agttctacat taccaaatgt gtttcctaac aatggtccta
    19981 aagtagaagc gaccattacc atacctggga acatttgcac ccataatagc catgcaacga
    20041 caaatcccca tttgctgccc agtgctgttt ttacccataa ctgaggaccg ccttcttgag
    20101 gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg accggcaaaa aacaaaactg
    20161 caaaaattga ataaaaaatc aatgtccaac cgactgcggc taacgtagga atactccgaa
    20221 ccgttgcaca cagtgccatt gtcagaccga taaaggtccc aagacccatt ttacttactt
    20281 cagatttact caccgttgac actctccttg attttttgga taaaataacg gtgtctttga
    20341 agtagtttca gcaataaacg aaacaattca gcgaatattt gaggggattc tttgagtcta
    20401 ttgcttgaag ggtttcttca aagacaacct caaaacaaag ggatcgaaga gcttaggtct
    20461 ccccttgctc ttcagatcca aacatgcgtc agaaacagtt agtattcaag ctgttttcta
    20521 acatgattac cttgcttatt ttgcttcgct tgccaataat tggtctgcgt agattttttc
    20581 caatttttct tgaagagctg ctttgatttt ttcagcatat tcttcaaagt gttcttcttt
    20641 gttcatgtat ggtgtcatta cgctggcacg caatacagtt acttttccgg cacggttcca
    20701 ttcttcatct gagaatccta gttggttcac aaattgtaat gggctgttgc catagtctgg
    20761 gatagcaaag tcagtatgtg aagttaagaa ctcattaccg taaatgcttc ctttcacgta
    20821 tgaagaataa tcatatacgt catggttcaa tttattcatt gcaactaagt catcgttgcc
    20881 tttttctttg aatacatagt ctaccatatt gaaatctgga taagttaatg gatgaacttc
    20941 gatttctttg tctcctactt taaatgacaa gtcattcaag aagttgtaga aacgatgtgc
    21001 accttcgatt gatgcaccca ttaatttacc atatcctgtg acattcaaag gtaagacatg
    21061 gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt gaaccttcca agatataagc
    21121 cccaagtaat gctgggatat ctgcgccttt ttcaaataca tatgtcgcaa agtaagagat
    21181 aacgtctctc atgcggatat ctttgatgac gataccaccg gcagaatatg gaacatagcc
    21241 cattttatgt gggtcaatcg ttacagattc tgcttcttcg attgctttat atgcactgtg
    21301 tacttcttct aagatataat ctttgttttc tgtaaagaca ttgtatttgt aatgaacatc
    21361 ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct aagaaaatcg cacggccgta
    21421 tccgccataa gcagcatcta cgtgtaagta gaagtaaata ccgtcttttt ctaatacgcg
    21481 acgcaatgca acgattttat cgataccgtc gattgctcct tcttctgttg atcctacaac
    21541 accgactaca cctaggattg gtgttttttc agctgctaga ccacgaacga ttttttcaag
    21601 ttcgttgatg tccatgcgat agttgtggtc aactggcact ggaatcactt ggtctaaacc
    21661 gataccgatg atgtcggcag cttttagcca tgaatagtgt ttagtttgtg gaaccaacca
    21721 ttttcctaat ttttctaaat gttttccgct acgtgctgag tgagctttga tttcgtcgat
    21781 tttttctggg acgctgtcta acagattcat gatttcttct gttgacaagt tcattaattc
    21841 ccaatcgctt tttccagcaa ctagttcagg tgtcacttct ttcattgcaa gcggcaatga
    21901 tttgatgttg cgggcatacc aaagtccttc taagtttgct aaagaaccat cagcaacgat
    21961 gtggccccag ccatctttat agctcattaa ttttgcaaat tccattccta cttcttcttc
    22021 catttggctt gttgcaggag aagattcata ggcaacgtta ttaccattcc atagcatagc
    22081 aaaattgtac gctagtaaag atggcatcaa tgtttcagag ttcatatgac cccagtaacg
    22141 tccggcatta tgccaaggaa cggaatgggt acgcatccgc gctgaaattt ctgatagaac
    22201 atcctttgtt ttattcacag tgtgttcaaa actagcactg cttttttctt ctggcgtaat
    22261 aataggcatg tcttgaggca tgtagttttg acgccagcct aaatgttcat ccaccaattc
    22321 attcaacaaa gctttataga tttggccgtt ctcagctttg tccccaatga atagggcatt
    22381 taggtttaga tctttcgaca atgattcact catagtgaaa ccctcctaaa aaaatattta
    22441 aattttgtcg agccctacat tacacctgtt cttctattct gtaaactaag ttttggcctg
    22501 atttttaatc atattttaat atgtatcaat tatccgaatt gttcacactt ttgaaataat
    22561 taaaaaaaac gagggaattt acaaatgtaa actccctcgc aaagattgta ttaacgacaa
    22621 ccagcgttct attttaggat gggtttcaaa attggaatgt tgtaatagta tttccctttt
    22681 tcaaggtaaa aacttactaa attctaatca attactttgg ccaaaaagta atttttctta
    22741 ccttttcgaa taaccacaaa cttaccatcg aattcatcgg atggatttat gacaaaattt
    22801 aaatcggtaa cgcggtcacc gttaatactt atagctccgt ttgaaacatc ttcacgtgct
    22861 tgacgtttag atggttcaat ttttgtggaa acaagcagtt ccacgatatt ttcaggtgtg
    22921 cttgaaattt caacgttcgg cattttaccg aatccttgcg cgatttcttc cgcatttagt
    22981 tctttaatat ttccagagaa caatgcttgc gttatttttt gcgcttcttt caagtcttct
    23041 tcactatgca caaatcttgt aacttcttct gccaaacggc gttgtgcttc acgtttctca
    23101 ggttctgtct ctactttttt cgctaagtct tcgatttctt cttgagacaa gaaagtaaag
    23161 aatttcaagt atttcactac atcgcgatca tcttgattca accagaattg gtagaactca
    23221 aatggtgaag ttttcttcgg atcaagccaa acggcaccgc ctgcagtttt tccgaatttc
    23281 gttccgtcag ccttcagcat caatgggatc gttaaaccga acgcttctac ttctggtcct
    23341 tctaattttc ggattaggtc taatccagct gtgatatttc cccattggtc tgctccaccg
    23401 atttgcaatt gcacatcgta tgttttatgc agatgcaaaa agtcgataga ttgcagaatt
    23461 tggtaagtaa actctgtaaa tgagattcct acttccaaac ggctggcaac gatatctttt
    23521 gctaacatcg tgttgatgtt gaagttttta ccgtagtctc tcaaaaattc aagtaaagag
    23581 atttttgaca accagtcgta gttgttcaca aatgtgatat ttgcatcttt accaaacaat
    23641 tttctcattt gattggaaag tgcgtccacg ttatgttgca cttgttccat cgtttgcaac
    23701 acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc ctgttccgcc gccaatcaga
    23761 atatatggat gatggcctgc caattggaat cttttcatca tcataaatgg aatcaaatgt
    23821 ccgatatgca tcgaatcacc agttggatca acgccgcaat aaagtgaaat gctcttttct
    23881 tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat taatggcgtc gcgccaagtt
    23941 aattcatcaa taatattcat tttactgacc tccgtgtagt ttttttatga aagcaggaac
    24001 ctgctagcat gtgtaattaa ttatttcttc aggataggat aggaaataaa aaaagtccct
    24061 tggcatataa gccaagggac gaaatctcgc ggtaccaccc aatttgtatg tctagatatc
    24121 caacatacct ctcgaacatt taacgctgtc gcgtggcaat cactgccaaa gactcctgaa
    24181 atgtaattcg ctttaaagca tgtactggtt tgcagcgacc accagctttc tgaaacattg
    24241 gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt attaatatgt gtctatcata
    24301 gagcgcaact ttttctttgt caacagaaaa aggatatttt ttttaaattc ctcatttttt
    24361 cttttttgaa tccgctctaa ttccgaacat tttattttca attattgtta aaagaaataa
    24421 ctttttctgt attttttctg caatttatcg agaagtcact ttactttgcc tcttattctt
    24481 tcttcgttac aataaggaat gggacaagta aaaaaatgaa aaagcaaggg acgaggtata
    24541 tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc tttaactatc gttattttaa
    24601 ttctaagttt gttttttgtc atccgatcat ctacaaaaaa agaaccaatc attcctgaaa
    24661 aaaaaataac agaaaaagca aaagaaccga cagatgataa gcaacaacta gttactcaag
    24721 caaaaaatct ggctcaaagc tacgattatg accaagcgat tgcgttattg gaaaaagata
    24781 gtaagaacgg atctactgaa acacaacagc ttcttcaaac actaaaaaag gagaaagaac
    24841 aactgatcga ctggtctgat cctactcaga tctctcatgt ctttttccac agcttgatcg
    24901 tggacccgca aaaagccttc cactctcaac aggcacaagg atacaaagat tatatggtga
    24961 ctgttgaaga attcaaccgt tcgatcgaac aactttatca aaatgatttt gttttagtta
    25021 atttgaatga tttgatccaa aaagacgaac aaggaaatct ttcctttaac gggcttgcgc
    25081 tacctgaagg aaaaaaacca ttggttcttt cacaagatga tgtcagctat tatgaatata
    25141 tggataattc cggttttcct agccgattgg tcgtatcaaa taacaatgaa atcaaaaatg
    25201 tctataaaga gaaagataaa gaagaaatcg gtgactttga catggttcct ctgatcgatg
    25261 cctttgtcaa gaaacatcct gatttttctt atcgaggcgc aaaaggtacc ttggccctga
    25321 caggttacaa cggagtttta ggttatcgaa catcaaaatc tcaatatgga gacaatgaaa
    25381 aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga acagttgaag aaagacggct
    25441 ggagctttgc ttcccataca tggggtcact tgaatatgac tcaagcttca ctagaagatg
    25501 taaaaaaaga caatgaattg tggcagcaag aagttgcacc tatcctagga aaaacaaata
    25561 ttctgattta tccatttggt gcagacatca gcgactggca gccttattca gcagaaaatc
    25621 aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt ctgtaatgtc gatgcttcta
    25681 caccagcttg gggacaatta ggtacggatt tctatcgaaa tgctcggatc aatatcgatg
    25741 gcattcgttt tcaatctgat ttagacggaa aaaatcctat tcttgatcca ttcatcaatg
    25801 tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg attttacgtc atttatgaac
    25861 caaaaaagcc gaactgacat tttatcgtca gtttcggctt ttttatgctt tatttcttct
    25921 tcggtgcgat tcccttgatc aggacttctg tccattctgc tgtatatgga agcagttttg
    25981 cgtatatctc cggcttagaa attccttcta ccattgcttg aaaatagagg atcaagaact
    26041 catccgaata tttcaaatcc acttttcctt ctttccgccc tcgatggaaa agatcaagca
    26101 tgatactgaa actttcttgc gtatacgcat ccatcgcgtc agaaaattct tttttttctt
    26161 tttttgtata gtatgtcatc aaatcgatat aaaattgttt gttcactcga ttgagataac
    26221 ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa tggaagattt tccgcaatca
    26281 acgctttcgc ttcttgccct aaatcattca taaatgcaaa aaatacttca taaatcagcc
    26341 cttccttgct gccaaaatat ttaaaaatcg tcgctttact tacctgagcg ttctcagcta
    26401 ccttatccat tgtcactcca gcggttccta tgtctgtgtt catcaagtca aaagctgctt
    26461 gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa accgttcatt tgtagagcct
    26521 cccgtaattt taaactttta taatatatat a
//
LOCUS       DAFFSV010000026        26307 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFFSV010000026 DAFFSV010000000
VERSION     DAFFSV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.026"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            237..1877
                     /locus_tag="LR110_001004"
     CDS             237..1877
                     /locus_tag="LR110_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBL4822004.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="LR110_001005"
     CDS             2031..3353
                     /locus_tag="LR110_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBL4822005.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="LR110_001006"
     CDS             complement(3415..3846)
                     /locus_tag="LR110_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4822006.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="LR110_001007"
     CDS             complement(3910..4746)
                     /locus_tag="LR110_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBL4822007.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="LR110_001008"
     CDS             complement(4936..5238)
                     /locus_tag="LR110_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBL4822008.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="LR110_001009"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="LR110_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBL4822009.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="LR110_001010"
     CDS             complement(5692..5958)
                     /locus_tag="LR110_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBL4822010.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="LR110_001011"
     CDS             complement(6212..6937)
                     /locus_tag="LR110_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBL4822011.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="LR110_001012"
     tRNA            complement(7303..7373)
                     /locus_tag="LR110_001012"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="LR110_001013"
     CDS             7490..8242
                     /locus_tag="LR110_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL4822012.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="LR110_001014"
     CDS             8258..8737
                     /locus_tag="LR110_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL4822013.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="LR110_001015"
     CDS             complement(8988..10568)
                     /locus_tag="LR110_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBL4822014.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="LR110_001016"
     CDS             complement(10561..11304)
                     /locus_tag="LR110_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822015.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KRLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="LR110_001017"
     CDS             complement(11480..12256)
                     /locus_tag="LR110_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4822016.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="LR110_001018"
     CDS             complement(12504..13340)
                     /locus_tag="LR110_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBL4822017.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="LR110_001019"
     CDS             complement(13351..14280)
                     /locus_tag="LR110_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBL4822018.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="LR110_001020"
     CDS             14472..14825
                     /locus_tag="LR110_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL4822019.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="LR110_001021"
     CDS             complement(14899..15555)
                     /locus_tag="LR110_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL4822020.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="LR110_001022"
     CDS             15781..17715
                     /locus_tag="LR110_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL4822021.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="LR110_001023"
     CDS             complement(17801..19024)
                     /locus_tag="LR110_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL4822022.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="LR110_001024"
     CDS             complement(19018..20916)
                     /locus_tag="LR110_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBL4822023.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="LR110_001025"
     CDS             complement(20910..22028)
                     /locus_tag="LR110_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL4822024.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="LR110_001026"
     CDS             complement(22111..22944)
                     /locus_tag="LR110_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4822025.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="LR110_001027"
     CDS             complement(22937..23854)
                     /locus_tag="LR110_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4822026.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="LR110_001028"
     CDS             complement(24081..25205)
                     /locus_tag="LR110_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL4822027.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="LR110_001029"
     CDS             25388..26296
                     /locus_tag="LR110_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4822028.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         8173 a         5214 c         4421 g         8499 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaatctt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttt
//
LOCUS       DAFFSV010000027        25513 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFFSV010000027 DAFFSV010000000
VERSION     DAFFSV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.027"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(54..788)
                     /locus_tag="LR110_001030"
     CDS             complement(54..788)
                     /locus_tag="LR110_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL4822029.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="LR110_001031"
     CDS             1115..1525
                     /locus_tag="LR110_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBL4822030.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="LR110_001032"
     CDS             1518..2219
                     /locus_tag="LR110_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBL4822031.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="LR110_001033"
     CDS             2219..3805
                     /locus_tag="LR110_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4822032.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="LR110_001034"
     CDS             3824..4045
                     /locus_tag="LR110_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBL4822033.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="LR110_001035"
     CDS             4042..4803
                     /locus_tag="LR110_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBL4822034.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="LR110_001036"
     CDS             5183..5692
                     /locus_tag="LR110_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL4822035.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="LR110_001037"
     CDS             complement(5767..6306)
                     /locus_tag="LR110_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBL4822036.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="LR110_001038"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="LR110_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBL4822037.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="LR110_001039"
     CDS             7319..8170
                     /locus_tag="LR110_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBL4822038.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="LR110_001040"
     CDS             8198..8833
                     /locus_tag="LR110_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBL4822039.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="LR110_001041"
     CDS             8853..9626
                     /locus_tag="LR110_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4822040.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="LR110_001042"
     CDS             10034..10831
                     /locus_tag="LR110_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBL4822041.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="LR110_001043"
     CDS             10809..11222
                     /locus_tag="LR110_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL4822042.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="LR110_001044"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="LR110_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL4822043.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="LR110_001045"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="LR110_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBL4822044.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /gene="maf"
                     /locus_tag="LR110_001046"
     CDS             15999..16559
                     /gene="maf"
                     /locus_tag="LR110_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBL4822045.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="LR110_001047"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="LR110_001047"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBL4822046.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="LR110_001048"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="LR110_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBL4822047.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="LR110_001049"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="LR110_001049"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBL4822048.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="LR110_001050"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="LR110_001050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBL4822049.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="LR110_001051"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="LR110_001051"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBL4822050.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="LR110_001052"
     CDS             21906..22484
                     /locus_tag="LR110_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4822051.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="LR110_001053"
     CDS             22536..23018
                     /locus_tag="LR110_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBL4822052.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="LR110_001054"
     CDS             23135..23509
                     /locus_tag="LR110_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822053.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24187..>25513)
                     /gene="brnQ"
                     /locus_tag="LR110_001055"
     CDS             complement(24187..>25513)
                     /gene="brnQ"
                     /locus_tag="LR110_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL4822054.1"
                     /translation="YLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWANLGFLVTGI
                     GLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPRLATTSFEIG
                     LAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLVLLGALLLLA
                     FLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTIQNMGVTKPA
                     EIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIADHYLGTYGS
                     ILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLAANVGLTNII
                     QFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDGLNACPETIK
                     QTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD"
BASE COUNT         8378 a         4626 c         5416 g         7093 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcacagcat gacagcattg
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc actagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tttgcgaaaa ctatttttat atcatatata tgtctaaaat ctatattata
    23881 aaagaaatgt tgtttgatca gcttctcttc ggtattagtt caaaacaaaa cggcaaagta
    23941 tatggagcta ctttgccgtt ttgtttctct aatactcagg tcagtttata gacgctcaat
    24001 aaacggtgtt caaaagccag tttctcggta ttaagtcaaa tttatgcaaa aatgtgagaa
    24061 gccattttca aaaaatattt cttaatactc gaaactgagc ggctttttca cgacctctca
    24121 ataaacagtg ttcaagagac agcctatcgg caataaggct gaacgtctct ttcacaacct
    24181 cttttatcaa tctgctactt cttctttctt agcgaaactc caaatcagtc cgataacaaa
    24241 accgataact gctggtacga tccaccccat ccccaatttg aaaaacggca agtagctttc
    24301 cgcaacatat aaaagatttt gaacaatagg tgtttgtttg atagtttctg ggcaagcgtt
    24361 caacccatca aaaatcgaag caatcaatgt aaaataagta gtcattcgat aaacagccgt
    24421 acgctgctta aacaaaggac caactaacac tagtaaaatc agtgtcattg ctaatggata
    24481 gatgaacatc aaaacaggca acgaaaattg aatgatattc gtcaagccta cgtttgccgc
    24541 taaacaagga aggatactag caagtgcaac aaagaatgcg taatttcttt gcggaaacaa
    24601 atctgtgaat gtttctgcaa aagctgtaat caaaccaata ccggtcttca agcaagctaa
    24661 tataacgata aatgctaata agatactgcc ataagtacct aaataatgat cagcaatctg
    24721 agccagcgtg atcccgccat tttcacttag agcaaagcta cctaagctca tagcacctaa
    24781 ataagcgagc aaagtataaa tgattcccat caatataatg ctgatcgcac cagatttgat
    24841 cgtgtcttta gcgatttcag caggttttgt tacacccatg ttttggattg ttgtgacaat
    24901 gatgatccca aatgctaaag cagcaagcgc atctaatgtg ttatatcctt gagtaaaccc
    24961 tgtgaaaaaa gcatgctctt gataatttgg ctgaataggc gcttgattta cagaacctag
    25021 tggattcaaa aaagcaagga gaagcaaagc acctaataaa accaaaaatg caggattcag
    25081 gaactttcca acataatcta atatctttgt cggttttctt gaaaaccacc aagccagcac
    25141 aaaaaataca atactgaaaa ttgctagata tagcgtctgt ctgctaggat cgataaatgg
    25201 tgctaaacca atctcgaaag atgtggttgc aaggcgaggt aaagcaaaaa acggtccgat
    25261 aactaagtaa agcaagatgg taaaaaccat cgcatatgtt tgatttatcc gttttgctaa
    25321 ttcatataca cctgagcttt tcgatacacc aatggcaatc acccctaaga agggaagacc
    25381 gataccggta accaagaaac ctagattcgc ccaaaaggta gcagcgcctg cttcttgacc
    25441 caaatgaacg ggaaagataa gatttccagc accaaaaaat agaccgaaaa gcatggagcc
    25501 tataaaaaga tag
//
LOCUS       DAFFSV010000028        25247 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFFSV010000028 DAFFSV010000000
VERSION     DAFFSV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.028"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            395..1306
                     /locus_tag="LR110_001056"
     CDS             395..1306
                     /locus_tag="LR110_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL4822055.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1523..1960
                     /locus_tag="LR110_001057"
     CDS             1523..1960
                     /locus_tag="LR110_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822056.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            2055..2939
                     /locus_tag="LR110_001058"
     CDS             2055..2939
                     /locus_tag="LR110_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL4822057.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            3163..6330
                     /locus_tag="LR110_001059"
     CDS             3163..6330
                     /locus_tag="LR110_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822058.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            6350..6895
                     /locus_tag="LR110_001060"
     CDS             6350..6895
                     /locus_tag="LR110_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBL4822059.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            6923..7378
                     /locus_tag="LR110_001061"
     CDS             6923..7378
                     /locus_tag="LR110_001061"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBL4822060.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            7375..8133
                     /locus_tag="LR110_001062"
     CDS             7375..8133
                     /locus_tag="LR110_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL4822061.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(8219..8959)
                     /locus_tag="LR110_001063"
     CDS             complement(8219..8959)
                     /locus_tag="LR110_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4822062.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            9020..10273
                     /locus_tag="LR110_001064"
     CDS             9020..10273
                     /locus_tag="LR110_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL4822063.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            10595..10822
                     /locus_tag="LR110_001065"
     CDS             10595..10822
                     /locus_tag="LR110_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL4822064.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            10945..11271
                     /locus_tag="LR110_001066"
     CDS             10945..11271
                     /locus_tag="LR110_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL4822065.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELVDVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11339..12052
                     /locus_tag="LR110_001067"
     CDS             11339..12052
                     /locus_tag="LR110_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL4822066.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12362..13213
                     /locus_tag="LR110_001068"
     CDS             12362..13213
                     /locus_tag="LR110_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL4822067.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            13334..15199
                     /locus_tag="LR110_001069"
     CDS             13334..15199
                     /locus_tag="LR110_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL4822068.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15247..16656
                     /locus_tag="LR110_001070"
     CDS             15247..16656
                     /locus_tag="LR110_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBL4822069.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            16764..17465
                     /locus_tag="LR110_001071"
     CDS             16764..17465
                     /locus_tag="LR110_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4822070.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            17624..18103
                     /locus_tag="LR110_001072"
                     /pseudo
     CDS             17624..18103
                     /locus_tag="LR110_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            18951..19525
                     /locus_tag="LR110_001073"
                     /pseudo
     CDS             18951..19525
                     /locus_tag="LR110_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
     gene            19525..19965
                     /locus_tag="LR110_001074"
     CDS             19525..19965
                     /locus_tag="LR110_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL4822071.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            19981..20682
                     /locus_tag="LR110_001075"
     CDS             19981..20682
                     /locus_tag="LR110_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL4822072.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(21170..21595)
                     /locus_tag="LR110_001076"
     CDS             complement(21170..21595)
                     /locus_tag="LR110_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822073.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            21969..22940
                     /locus_tag="LR110_001077"
     CDS             21969..22940
                     /locus_tag="LR110_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4822074.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            22972..24369
                     /locus_tag="LR110_001078"
     CDS             22972..24369
                     /locus_tag="LR110_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL4822075.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            24528..25151
                     /locus_tag="LR110_001079"
     CDS             24528..25151
                     /locus_tag="LR110_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822076.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         8615 a         4148 c         5323 g         7161 t
ORIGIN      
        1 tggtagtttt aatatattca tttttagttg tataaaaata tatcataata aaaacaacaa
       61 aaaaatgtct ggaaaatcga acataaaagt gtctgatttg aatatcccag cagcggaaga
      121 agaaaaacaa aatcgataac atttgttcta agaattagtt acgttaaata cggaacgcaa
      181 gatagacttg aaaaacggta acaaaagaag tagtatatta aggtgtgatg tgctacttga
      241 catttattct gctgagcgaa ctttctggat tgtgtagcct tatatctatt caggacgtat
      301 cgagcagatt agtaaaggtg ttcttgatga ttttcatctt gaacaccttt gtttttagca
      361 attcgcaaaa aacgaaaagt aggagaattg ataaatgaaa aaaagatttt tagcacttgc
      421 catcgtacta ggcactggct tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt
      481 tagttataaa ggcggtacaa tcagcgaaca agaagtaatg gatagcttga aaaaaatgca
      541 aggagcagat tccgctgtgc aacagttgat cgtttatcaa gtatttgaag ataaatatgg
      601 agatgacgta tcaaccaaag aaatcgattc tcaatatgat caaacaaaga aacaattggg
      661 tgattcattt gattcacaat tgaaatctgc aggttacaca gaacaaacat tcaaagacag
      721 tatcaaacaa agtctagcct ttcaagaagg tctaaagaaa catatcaaat tgaccgatga
      781 agatttaaaa acggcttggg aatctttcca tccagaagtg gaagctcaaa ttatccaagt
      841 agcaagtgaa gatgatgcaa aagatgtgaa gaaagctgct gataagggag acgatttctc
      901 taaattagcc aaagacaagt caactgatac tacgacaaaa gaagatggcg gaaaagtgaa
      961 atttgattcc actactacaa cggttccggc agaagtcaaa gaagcagcct ttaagttgaa
     1021 agatggtcaa gtttctgatg tgatcacttc taccaatgct tcaacttata ctacggaata
     1081 ttatgtggtt aaaatggtga aaaaccaaaa caaaggcaac gacatggata aatacaaaaa
     1141 agaattgaaa gaaattgcaa cagacacaaa attaagtgac agtaccttcc agaataaagt
     1201 aattggcgaa gtattgaaag acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt
     1261 tttgagtaca ttcacttcag attctagtac agcaagtagt aaataagcgg tataaataaa
     1321 aaatatgaac tatttaaatc aataaaactg ttcatctaaa tctttttatt agaactaaat
     1381 caacagtagt ttaattacta cttgatttag ttctttttat tttaaaaatt ttttttactt
     1441 agaatccagc gaccctagat atttttctgt ataatcttaa ataaagaaaa caatatcaaa
     1501 aaatgcacgt agaagtagat aaatggagtg gttttgcaaa tttcttttat ttctagaagt
     1561 tattcacgta tacggatttg tttttctcac aaataaaaag gcaggaagta caatggatca
     1621 tttgtatata aaatatattc atcaattttc cgttttggtg ttaggcacac taattatagc
     1681 ggggattgtc gcatttatta ttcaaggccg tgacatatta acagtacatc aagagttttt
     1741 cagttatgca tttggtattc ctctgatcgc tataaggtct gataacaaaa agtggaagaa
     1801 acgctattac attctcttgc tatttttgtg tttttatctg ccacctaaga tttttacttt
     1861 attaggtata caatgcctat cattttcttt tatttcctat atcattgttt taattttcct
     1921 cagtttcata gtatggatgt tttatcttgt caaaaaataa atgaatgttt catgaacagt
     1981 acttcatcac agcctaattt ttttctttat gctatcgtta tattaaagaa atcaatcaga
     2041 agggaaagaa agtgatgaaa aaagttttat tagtggtaaa ccccagttca ggcggtgaac
     2101 aagcaaagga atttgagcaa ttagcgatag caaaattgga atcggtgttt gatgaagtgg
     2161 tcgtcttgca tacaaaaaaa gcaggggatg caaaaaactt tactcgcgaa gcggctacgg
     2221 aagggtatca tagtgtgttt gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg
     2281 gaatcgctga acaagaacat cggccaaatt tcggattttt cccattgggt acggtgaatg
     2341 acttggctcg agcattaggg ataccgctag aacctgagga agcgatcaat catttttcga
     2401 ttgaatcagt caagccattg gatattggaa aaataaatga cgactatttt atgaatgtcg
     2461 tcgcaattgg ctctatccct gaatcaatca atgatgtaga tgcggagaag aagacgaaat
     2521 ttggaaaact agcttatttc atgtcaggaa tcaaacagtt agcttccaca caatcctact
     2581 cgtttcacgt agaagttgat ggaaagaagg aagaaattga gagcagtacc ttgctgatag
     2641 gactaactaa ctcagtcggc ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg
     2701 gtaagctcca tttggtttat ttaaaagatt cttctttgat cgatacgctt aaaacattgc
     2761 cagacctatt aaaaggagta gatgaatcta cgaaaaatct agcctacctt acttgtgagc
     2821 aattagttgt gtcattagct gatgaggaag aattgacgac gaatgttgat ggagatgaag
     2881 gggataaatt acctgtgaca atcaaagtat tgccctcaca tttacaggtt tattgttaaa
     2941 atggaggttg tgaccgagga gttggcggga gaacaccgct tgttcgggat aagggactgt
     3001 gacaagactt ttgtcacagc cttttttttg tacaaaaaag aaatttacac ctaatagaaa
     3061 aatttttcca taaaaataca tattttaaat atgaaagtac aattggaaat agattaaaac
     3121 gataaaataa aattgtcaaa acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa
     3181 aagctgattg cgtcgatttt gtttgtcttc cctctcttga tggtgacatc cgtttttgcc
     3241 gatgtagaga aaacaaacga atcaacgcaa tcagagaatc aaagttttga caagactact
     3301 ttttcagaag aatcgattac ttctgaaact acagaatctc aaagtgaggc ttctactaat
     3361 aaaaggcaag aagaaaataa gacaagtgag acctcagaag tagctgaaca aacgtctgat
     3421 agcagtgaag cggcactggc aagagctgtt tcagcggatg ggtacagcga agctgccaca
     3481 acagaagaac tggcgcagct tctcgcagac gaatcagtaa ctaagatccg actgattcag
     3541 cctttgactt tagacaggga attagagatc aaacgagata tcgttattga ttttggtgga
     3601 ttcgctcaca atttcggcac acaccatatt tatatcaatg agatacctca gtcgatcgag
     3661 ttccagaatt ttaaaggaac agctgcacat ccaggtggat tgacaccaaa tatcgatgga
     3721 aatgccatta tcattgctta tatgaccgat ttttggggaa gcagatatca ttttacggga
     3781 gaaataaaat ttaccgggac acttgatttg tatgatgggt ccaaattagg actgatttac
     3841 gctcctcgtg caactgtcac attagatggt gtttccggtg tgttggatgt ccaaccagtg
     3901 aaggaaggac tgaatgccgc tccgggtaaa gcctattttt gtagaagcta tcaattgaat
     3961 gtgatcaatg gtagtcaact ttatggaccg tatctaggga aattctacgg ttttctagat
     4021 tcaggagatg agactggtag tggaaaaaca gcgccaggaa tccatatctt aagcggttca
     4081 aaggtctctc ttgattatga tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat
     4141 acgctacctg gtaatgtcat gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat
     4201 acaaagatca atattaccaa tgacaacaat cgcggaatca tccaaatgag aggttcagga
     4261 tcgaaagtca ctgtatcgaa tggtgggaaa atcaccatca atacagcaac tacgggcgga
     4321 tttcgcttgc aaggagattc tagtcaaatc catgtatcaa gtggtggaca aatctcggtc
     4381 aaaatggctg gagatgacga tcaacccggg aacaatggga tgcgttttgt aggaaaaggc
     4441 ctttctttga tcgtagaagg agccggatca gttatcgaca tcgataagca gagtgggcgt
     4501 tccagcgcag ttcgttttga gaatggtacg caaaagctga cagtaaccaa tggaggctca
     4561 ttgagggtaa gaaatacagg tgatggcaaa tcttacgtga acagactcaa tcaaggaaat
     4621 caagcgatcc aattttatga cgcttctggt tttctagaaa gtccaggttc agctgatttt
     4681 acagtcactg gagaaaaatc aaatatcgat atacgcgcag attccggagc aactgtagat
     4741 acaactggag atattgattt aaattttacg gcaggtgaaa aaacttattt agtcatggtc
     4801 gggaaaacag gggaagataa gaccggcatc atcagtgggg atgtgttgaa tgtggactta
     4861 gaatctccta cctattttga tttccaaaac aaacggattg gaagcgagac caatggcata
     4921 ggcggctggg tattccaagg aaatggagat tcttctttaa agatcgccca ttcagaaatc
     4981 gctttatggc gaaaaacagg cgcaacattc gataactttg aaaaagaccc ggattatttt
     5041 tctgatatga cagacttatc gatcaatggg accgacttag gcaatttcgt atcaagccct
     5101 agtgcaggtt taacaaatga atttgctcag acaggtgccg gaatgaaaaa ttataatcga
     5161 atcagtgcca ataatcagct cccgatgatt gaatccttaa gagtgccaac gaatgcagat
     5221 aaaaaaatct atgggcatgt cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct
     5281 tggacaggcg aagtagaagt gactttacgt attacttacg cagatacgac gaaatcatct
     5341 gtagaaatta ctgctctcac gcaaggacaa acagatgaaa atgaaggggc aggatataac
     5401 ccatggggag aaggcgaaca aggcggcttg ttcgaagttc aagtacccaa tgatgagaga
     5461 ttaaaaacgg aggatcaagt gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa
     5521 gtgacagaac tggactctgc caaaacgaca gtcgatgtga ttcctcctga acctgctaat
     5581 ttaagtacca cattgatcaa tgcagcttct cgcacgatca caggtacagg tgaagcagga
     5641 gccttggttt cattgaaaaa aaatcaagct gttttgtcag aaacaactat tgatgataca
     5701 ggggaatttg ttctttctat cccagaaaat caactttttg caggagatgt attagaaatt
     5761 gtattaaacg accaagcagg agaagctact gctaagggtg tgcagaacaa gccaagtacc
     5821 aacgatgaaa ttgggaatca caatccaagt gatacgccag ttgtttatca tgatgccccg
     5881 ccttttgcac ctgctacgaa gatcgtagta gaaggtggtc tctcatttct tgccccttcc
     5941 aaactcgatt ttggtcagat caaagcaact ggtttgacac aagaagcatt tggccaaatg
     6001 gatatacagg aaaaattatg gatttatgat cagcgagaaa cgaaaaatcc gtggatctta
     6061 acgataaaat tatctcagcc tttcacaaat gatgcaggat tttctatgca ggatgtccta
     6121 tatttcaaga aaggagagga gtatcaatta gtgaatgaag aagaactgga aattgtcaga
     6181 ggacaaaatt caacgaatgt gcaagcagat tattcagaat atttaaataa cggtcgggcg
     6241 tttaaacttg tcttaaccaa agaaaatcag atagctggta attactcagc agagataaca
     6301 tggacattag cagatgcgcc gacagaataa agatcaagga ggatgattga tgaatcgaat
     6361 atggaataaa agtcatcgaa acaactgctc tttacttatg atccttctcc ttggattcat
     6421 cctttggcag acacctgtcc atgcctccga attcaatttt gcggtagatt ttgaattacc
     6481 ggctaatcag attgataaga acaaaacata tataaatgta aggatggagc ctgggaaaac
     6541 acaggaaatc ccctacacgg tctttaaccg aacagataaa tcagtaacag ttgagacatt
     6601 gatccactcc gctacgacga attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa
     6661 atttgatgaa acattaagtt atgatatgaa aaacttagca aaaacagtgg gagaaataac
     6721 tgttcctcct caaagtgcgg tcactgagac actttcactg aaagcgccag aaaaagagct
     6781 ggctggtgtg gtcgcaggag ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa
     6841 aagtacagtt ggtgtcgtca atcagttttc tcaagtgaaa gcaatcttga tttgaaatag
     6901 cgaaaaagag tcttccccag atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg
     6961 aagaaatgtg attaaggcga atcttcaaaa tccaaatgct tcttatatgt tcggcttgac
     7021 tacatcggtt gaaatctaca aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga
     7081 aaaaatagat gtagcaccca atacaaattt tgatttactt gtcccattag aaggaaaaaa
     7141 attagctccg ggacattatc aagcggttgt gaagggtgct tggaaagaaa aacgctggaa
     7201 tctccatacc gattttgaaa taactgatga tgcagcggac caattgaatg aaaaggatat
     7261 tgatttaaaa aatcagccag aaacatttga ctggcgatgg ctgatacttg ttggagtcat
     7321 tcttattggc ttatttgctg gattatttat tttaaaagaa agaaggaaaa agaaatgaaa
     7381 aaaacaattg cattaagtac cgttctagct ggaagtctat tattatgtac acaaagtgtt
     7441 tttgctgaga ataagagctc agccactaca gaaggaacga tttctgtgac caaaccagat
     7501 gctgcaaatc ctgaagacgt tgttccagtt cctcctgttg atccaaatga tcctgatact
     7561 ccttttacac ctgacgaacc aaatcaggga acatccggat tactgacgat tgaccaagct
     7621 ccagatcttg attttggaac aatcaagtta ggtacagcaa aaacagtata tgctgcatta
     7681 caaagcggaa cggacagtac tggagcacaa acacaagtac agaatttcgt acaagtaacg
     7741 gataaaagcg gaaattatgc cggttggaaa ttatcagtca aacgaacaga atttattggt
     7801 acaacatctg atacgaataa actaactggt tcaaaggtga aattcaaaaa cgccgtgatt
     7861 cgaaatagcg caactaatag cagtggaatg ccatctaccg tttctggaac tggcgctgaa
     7921 ggaatcgaaa tcccagcaga tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg
     7981 attggcacat ggatttattc attaggagag aatgtggaac aagggagaca atcagtggaa
     8041 ttatttgttc ctgtatccaa ttatgctgta gacaactacg tttcaacatt cacatggtcg
     8101 ttaacagatg ctcctacaga agaggaagcg taaaatcata tcaacgattt cagggagtgg
     8161 agccgattca aggcccactc cctgaaatct gctatgctta cgtctcagct cttattcatc
     8221 aactcgaagc agttttttgg aagtcatgga aacgttcctt tgtcttttca aagatctttc
     8281 gaccaacgag taaggagtca ccgttgaaaa attctaacac ataatgagca gaatcaattt
     8341 tctgcaattg cgataagttg atacagtaag attttaaaag taaaaagtaa gcatactttt
     8401 ctaactctgt cttcaattgt tttaaagaag tattcactaa tttatcttct gtagttgttt
     8461 tgaccataac ttggtttttc aaacctttga tactttcaat atataaaatt tcccgaggat
     8521 acaggacgag acttccacct ttttgatcaa taaagatttt atcgacattt acgatctgct
     8581 gttctttcca attgatctgt tctagcactt gacgaatttg ttcagataaa ttcctgtctt
     8641 ttctgatata tccaatcgga aggattttcc gattgatgat ctctactgca aaattcgtat
     8701 aagcggtgaa aaaaacaata ttactatgtg catttttttt acgtatttgt tcggcgacat
     8761 caatacctgt gagtgattgc ttcaagtcga tatcgataaa gaaaacatcg tgattatcaa
     8821 tagtgcccaa ctggatcaac tgattgattt gagagataga agtgagctgc acgatattct
     8881 cgatatgatg attgaccaat tctctgatga tccattcgct gtgaaactgg ttatcttcta
     8941 tgataaaata ctttttcata tgcaggctcc tattaaaaaa atatgtgtac attttgaaac
     9001 aaaacaatgg aggaataaga tggtcgcaac agtgacactt tatttgacct ttactttgat
     9061 tttagattta ggtatctatt tattcttttc gaaacaactg ctagtaaaaa acagctttgg
     9121 ttgtatagct ttgatggtgg tgacactttt acttcctatc cctaaagtga gctttcctta
     9181 cctagttggc ttattattga tgtttctttt ttattttact acgaaaaacc tgtttatcag
     9241 ctgtttgatc agtaatcttt tttttgctat cactggactc agcttttttc tggtttttga
     9301 tctgatggat tatttaggaa agagacatct tggaacagca agtttttttg cactacttat
     9361 cgcatttatt ttctatctat tagtaaaaag tatagacaaa cagacgagat ttctcaactt
     9421 gctttttact tatccaactg aacactattt tggaggtatt tgtattttat taagctgggg
     9481 cgtgatttac ttggtattgg gaagtgttca cttccgctca tctaattatc tgcttctatc
     9541 tcttttgtcg atagtcatca gttttttttc tttgtttttt tccattatgt tgatcagttt
     9601 ccaagtaaaa gaaagacaac atcaggaatc gcttcaactg tatcaaataa atgaagaata
     9661 ttaccatcat ctagaggaat tcaaacatga ttataaaagt ctactgtttt cattaaaaat
     9721 tactcttcag gaaaataaaa aagaaacgtt ggaattttta gaaatattag aagatcattc
     9781 tgcagctatt tttgaaaaac cgcaaataca acagctaaca aatattgtat ccccagctgt
     9841 caggggtgtc tttttaaaat ttctagaaaa agcagaatcg gagaaaatcc cgatccaagt
     9901 agttattcct gtcgaagtga aaacaatccc gattgatctg gttgttttta ttcgctgttt
     9961 atctattttt atttctaacg cattcgatca tcgtgtagcc gatcagagtc cgattacgat
    10021 caatctgctg gaagaaaaag agggatcacg ttttagtata gcgaacaagg caaatccaac
    10081 gaatttgacg ctatcggaga tggtcaaaag aggaaaaacc tctaaaaaaa atggcgggtt
    10141 aggtctatac agtgcgaaaa aaatgttgga tgtttacgaa aatgcagaac taaaaatcga
    10201 attttcaaaa cagaatcact atttttttgt agaaatgtat ctggcatcta gaaagagcaa
    10261 atcagcctac tgacttactg tcaaaggata agtttgagga aagtctacac ggagatagag
    10321 acaacaacac agcatattca aaagaaacag aagcgtgatg accgttgtca cccttctgtt
    10381 tcttttgcta tgtagttaaa caaagtagtt gacacttccg ctacttagta ttagtacgaa
    10441 agtatacttc atgatactaa gtagataaga agtgaaaagc ggaacccacg atcgtcaaaa
    10501 tgctaactgc tttgcttaca ccagataaag ggaagagcaa tatcgcttat tttaggcgtg
    10561 gagaagcaag caaatcccta cgatcacaaa aaaaatgctg acccagcagt taagagagtt
    10621 agaacaagat gaaagtatcc atcgaaaagc ctataaccaa gtcccgccta aagtagagta
    10681 ctcgctcacc gaagaaggaa aatcattaag agagattcta gtagcgttgt ctgtttggga
    10741 agaagaacgt atcgaaaaac aaaaagcctc aagggaagaa gacacgctac tgcatacaca
    10801 tggtggctac ctaaattatt gattttctat gacatttatt ttaaataaaa gaaaacagga
    10861 gtttttttaa aagcatcatc tattttttat gtaaaaaaat ggtatgatta atcaagtaag
    10921 aagtgattgg aagaaggcaa gcaaatgaat agtatggaaa aagtcaacca gtttagagat
    10981 gaacgtaatt ggcgacaatt tcataacgaa aaagatttgg ctatctctat ttctttggaa
    11041 gcatctgaat tgttagaact ttttcaatgg aaaacgccgg aagaagtaaa agaaacacaa
    11101 ttagaacgaa tcaaagaaga actggtagat gtattgatct attcatacat gatggcagac
    11161 aaccttcacc tggatctaga tgagatcatt gaagaaaaat tgatcaaaaa taatttgaaa
    11221 tatcctgtag aaaaaagcgc aggtgtacga aaaaaataca cagagttgta gaaatgtgtc
    11281 attcggctgt tggaaagagc aaaagaaaaa cagtaaaatg agtctggagg gagagaaaat
    11341 gaaaaaaaaa ggaaaagcaa aaagatggct agtcaatatc ctcttgttcc tcttgttatt
    11401 agtaggacta gctttgattt tcaatgaaca gatcaaggat tattttgtca gagagacagg
    11461 agataaatat gctatagcga atgtaacaaa agaagatctg aaaaagaata acgataaaga
    11521 tgtcagtttt gactttgatg cggtagaacc aatgacgaca gaaggggtca tgaggtcgca
    11581 aatgagagga acagatctac cagtgattgc tagtatcgca gtcccttcag tttcaatcaa
    11641 tttaccggtt ttcaaaggct tggataatac gtcactactt tacggagcag gaacattatc
    11701 tccagatcaa gaaatgggaa aaggaaatta tgccttagca agccatcgtg caacgaaccc
    11761 tgaattgctg tttacaccgc tagaaaattt agagatgggt gccaagatct atttgacaga
    11821 tttagaaaat gtgtatacat acaaaacatt ttttaaagaa aaagttgctc ctacagatac
    11881 ccaattactg aatgaagtcg agggaaaaga aattgtcaca ctaattactt gtggcgacat
    11941 ggacgccgtc acgcgactag ttgtacaagg agaactggaa agtgttacat cgataaaaga
    12001 tgcgacagat gatatgagat ctgctttcaa tctagagaca aaaacatttt aggatactgg
    12061 gacataagtc gttacagatt aaaaacagcc aaaggatatg aatcgataag gaaaaaccaa
    12121 tttgctgaaa aaaagagaaa cgcaaagagg tgcatctctg atttggttgt tttctagata
    12181 gacttgtgtt ccagtttttt tattttttat atgaaaaggc ttgcataaat aaaaattcga
    12241 tggtatgatg attttgaata agaaatggat tgttactgta cggcaggcaa aaccagaatt
    12301 cagctaagaa acagtaggcg aattctaggt ttttttattt ttcttgagag ggcgaagcaa
    12361 aatgaaaatt gataaaattt tgaataacaa tgtcgtcatt tcgaaaaatg gattcgggga
    12421 agaagtcgtt tgtatgggaa aaggactagc ttttcaaaaa aagattgggg acgagatttc
    12481 gccagaagcg gtccaaaaag aatttgtttt aagagattct tttgctaaaa accaattcca
    12541 gcagctgatg gctgatattc cggcagagga gatcgaatgg gtcaaacaag tagtcgaact
    12601 ggctgaagat aaactaaaag ttgaattttc gcctaatatt tatctgacat tgaccgatca
    12661 tcttcattat gcgatcacac gagcaaaaga aaatatccaa ctgcctaatc cattattatt
    12721 tgaaacaaaa aaattttatc cgaaagaata tcaagcagcg aaagaggctc ttttgttcgt
    12781 gaaacaaaag acgggtgtgg aactttctga ggatgaagcc ggttttattg cctttcattt
    12841 tgtgaatagc caacaaggaa acgaagacat gcaagtgacg atgaccgcta ccacaatggt
    12901 aagagatata ctgagtatca tcagtaaatt cttcggcatc gtgttcgatg aagaatcttt
    12961 gaattatcag cgtatcatta ctcatctgca gttttttgcc cagcgttatc tcaaagggga
    13021 aggatctgac gagcaagatg aatttctcta cgcattagtt caaggaaagt atccaaaagc
    13081 gtttcattgt gtcgaacgga tcaatgagta tcttcgacaa acgcaaaaac aggaaatggg
    13141 gatcgcagag cagatttatt taacgattca tattcaaaga gtagtaagtg agaaaaaaag
    13201 catccaatca taaggattgt tactgtatgg caggcaaaac ctggattgaa aaaataggtt
    13261 cacgaatgtt tgtgactgtt tttttcaatc tgggttttta ttttttaaat aaaaaagaaa
    13321 gaaggaaaaa acaatggata atcaagcagt tggtcgtcgt gtatgggaag cggtaggagg
    13381 acaaaagaat gtcaaaagtt tagtacattg tgctacgcgg ttgcgattca gattaaaaga
    13441 tgaatcttta gcagatacgc aaaagttaaa agaagacccg gacgtgatcc aagtcgtaca
    13501 aagtggcggt cagtatcaag tagtgattgg cagtaatgta gcagatgttt atcaatcgat
    13561 cgtggatgag gaaggtcttg cagtttcgga ggatggaaaa gaagagtcca aaaatatttt
    13621 gaatcgtctg atcgatatta tctctagtat atttacacct tttctagggg cgatggcagc
    13681 cgccggggta ttgaaaggat tcttatcatt ggcaacggtg atgggctggc tgacagctga
    13741 tagcggtact tatcaaatat tgtttgctgc agcagatggt gtctttacct tcttacctgt
    13801 catgcttgct tttacagcag ctaaaaagtt caaagccaac cagtttttag cagtagcaat
    13861 tgctatggca cttgtgtatc cagctatcac agcagttgct ggtgcgggag aagctatcag
    13921 tttctttggg atcccagtga ttctttcacc atctggttat acttcttctg tcataccaat
    13981 cattctagcc gtatgggttc aaagtaaatt agagccattc gtaaaaaaag tgattccgca
    14041 atttctgcaa atgattttag ttccgcttgt cgttttagta gtgatggttc cattaacatt
    14101 tcttgcttta ggaccaatcg ggacggttgc cggtaatgct ttaggcggtc tcttcaactc
    14161 gatttatgga tttagtccaa tcgtagcagg tctaatcatg ggaagtttat ggcaagtatt
    14221 cgtgatgttc ggcatgcatt ggggattcgt accgatcatg tttttgaata tcgaacaata
    14281 tgggtttgat gtattgatgc caatgcttct tccagccatc ttggcacaag gtggggctgc
    14341 tttagctgta gcattgcgaa caaaagatac gaaacttcga gcattaggga tttcttctac
    14401 agtgacttcg ttatttggaa tcactgaacc aactgtgtat ggtgtgacat tacctttgaa
    14461 gaaaccattc attgccgctt gtatttctgg aggtattggt ggagctatta tcggattttc
    14521 cggagtaaaa gctttctcca gcagtttggt cagcttattg acgattccga ctttcattaa
    14581 tacggtggat ggtgtagagt caaatgtgac agtagcagta atagctacag gaattgcttt
    14641 tgttcttgca ttcgtaggta cattgatttt aggatttgac gaacaaacac aagacaatca
    14701 attagaaaac aaacatgcga atgcaggaga accgattact tcagcacggc ataccttaaa
    14761 aagtccttta acgggtaaag tactgccatt gagtgaagta ccagaccaag ttttttcttc
    14821 tggcgtgatg ggaaaaggta tcgctattga tccagaagta ggagaactag ttgcacctgc
    14881 agacggagag atcacgacta tttttccaac tggacatgct gtagggatca caacgactga
    14941 cggagcggag attttgattc atatcgggat ggatacagta gaattaaatg ggaatggctt
    15001 tgagatctta gtcaaacaag gggatctcgt caaagcagga gatttattga ttcgttttga
    15061 catcgaagca attagagcag ctggctatag cgtaatcact ccagttgtca ttacgaatac
    15121 tgatgcattt gccgatatac ttgaattaga ccaaaaagag attattgcaa acgaagatgt
    15181 tctggcaatt gtaaaataag gaggaaatca ggatgcaatc acgtttttca gaaaaatttt
    15241 tatggggtgg agcaactgct gccaaatcaa ttagaagggg cttatgatcg agacggaaaa
    15301 ggactatccg ttgcagatgc tatgcctgga ggtaagcagc gctttgcgat tatcggcagt
    15361 gaagaatttg actggacgat tgatcaagaa aaatatattt acccgaatca tcgaggaatc
    15421 gatcattacg atcgtttcaa agaagatctt gccttgtttg cggaaatggg attcaaatgt
    15481 tatcgttttt cgattgcctg gacacgaatt tttccaaatg gagacgaagg tactcctaac
    15541 gaagcgggat tagaatttta tgatcagctg attgatgagt gcttaaaata tgatattgaa
    15601 cctgtgatca cgatttctca ttacgaaatg cctcttcatt tggcaaaaga atacggtggc
    15661 tggaaaaatc gaaaactgat cgacttctat gaacgatttg ctcagacagt attagagaga
    15721 tacagcagta aagtcaaata ttggatgact ttcaatgaaa tcaattcggc ctttcacttt
    15781 ccagctttaa gtcaaggatt agtaaaaagc aatggagcag gagagtacca aaatattttc
    15841 caagcatggc ataatcaatt cgttgcaagt agcaaagccg taaaaatcgg ccacgaattg
    15901 cgttcggata tccaaatcgg ctgcatgatt atctatgcaa caacttacag catcgatgcc
    15961 aatccagtca atcaggcagc aacaatgatc caaaaccaag aattcaattt tttctgcacg
    16021 gatgtccaag tacgtggaga ataccctgca tacacagcgc gtacccataa aaaatatgga
    16081 gtcgatccgg aaaaattaga gcaaacggaa aaagacttcc agttgctaaa agaatatcca
    16141 gtggactaca tcggatttag ttattatatg tctacggcaa tcaatgagac tgatccacaa
    16201 gcagcaactt cagaagggaa cttgctggga ggagtaaaaa atccgtttct tgaggcaagt
    16261 gaatggggct ggcagatcga tccagaaggg ctgagaatcg cattgaatga attatacaat
    16321 cgttaccaga aaccattatt cattgttgaa aacggtcttg gagccattga tcatatggaa
    16381 gaggatggca cgattattga tgattatcgg atcgattatt tacgtcgtca tatcgaagcg
    16441 atggctaatg cagtagcaga cggtgtagat ttgatgggct atacgccttg gggatgtatc
    16501 gatcttgtca gtgcatcaac aggagaaatg agtaaacgtt acggctttat ctatgtggat
    16561 ctagacgatg aaggaaatgg gacactgaat cgatcaaaga aaaaatcttt tgattggtat
    16621 aaagaagtaa ttgcctcaaa tggcgtaaac ttgtaattaa agcagctgaa tgaaaaagaa
    16681 cacacttctc acatgtgatt tgtgttcttt ttttattttt tatgtgctat tcttttagta
    16741 atagtaaagc aaagggtgac gttatgaaaa ggacacgtgt actatacttg gaagtcgcag
    16801 ataaaattaa agaagatatt ttctcaggaa aatatccagt cggttcgatg ttgccgacag
    16861 aaagtgaatt agaagaactg tttcaagtca gcaaaatcac tgtccgaaag gcaatcgaac
    16921 tgctggcatc agatgaatat gtcgcaaaga aaagtggtcg tgggacgacg gttctaagca
    16981 atcgtcctta caatcgatta tccaaagcgg caagctttac gcaaatcttg gagaactccc
    17041 gatatgaagt ccggaaagaa acgctggatt tgcaattggt ccatataaag aaggaagatc
    17101 ctttgttttc ctttttcggt gagcaagctg tttgttttaa gcgcttatat tatttgaaca
    17161 atcagcctta tatttatttt gttcattatc ttccacaaga aatgatgcaa ttcagcagag
    17221 aagaatttgg caaagaatct ttgtatcgtt tgctgacgaa aaaaggatat actattgact
    17281 cattttctga tgatttctcg gcagtatttt taacagaaga agaacaagga attctgaaaa
    17341 cagacgcaca gatcggcatc aaacggatac ggaaatcgtc tacgcaaatt ggggaaatga
    17401 ttgaatattc ggaggcggtc taccatacgg agctgcatcc ttaccatatt gattatgaaa
    17461 cataaataaa aaaagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
    17521 acaaaaatag cttctcatat ttttcgtttg gttaggctta ttaccgcaga tacacggctg
    17581 tttctggatt accatttatt ctggataagg gggtgtggca cgattttcag aaggaatcta
    17641 tgaaccaatc actgtattga ttttagcttc gatggtaacc ccattcggct tgacgatcgc
    17701 ttatttctta gggaaaatca ttcgtaaaaa tatactcaat cgtcaagaaa tcgatacatt
    17761 gaagaccgct tttcctatgg ggatctgtca gatcacagag ggatgtttcc ctatcgtatt
    17821 gaacgacttg gcacgcaatg tcatagcaac gggagtcggt gggacagtag gtggcggatt
    17881 aagcatgttt tggggcgcag atagccgtat tccggcatcg ggaatgtttg cagtcgctac
    17941 gatgaccaga ccatgggcat tcattggtgc attgttagct ggttcttttg tcacaggaat
    18001 aaccatttta ttattgaaaa aaccagtaga tccgaatgca gaaatcatcc aagaagaaaa
    18061 agaggaagaa gacatatctt gggatgattt gacaattagt taggagaggc agaatggatt
    18121 acaagaaaaa agtacaatag atgtaccttg agacaggaat ggtatgaaag aatcagagtt
    18181 agaaagaatc gaatatacag attttggttt aggagcgatc gaaactgtcc agagcatcgg
    18241 caccctcctt gaaatgacga tgaaggaaaa atgagaacct ttcgttgtcg aaagggtctg
    18301 gtatacctta tgtcgaagga aaggaaacaa aaaaactttc ctgtactatt ccaaaaagga
    18361 aagaaagtta ttatacagta aaaaaagaaa ttattcttca tgctagagat cagtacacgt
    18421 tcgagcccaa tatcaaacac tagttccagg caggcgcaac aggtgcggtt atcactgaat
    18481 tttcttccag cagtgacgat tcaagtgata tttttacaaa tccagctatt tatcgttaac
    18541 aaaaagattt ttaggagaag ctgatgaaca aaaatgatca aattttgatc gtttagttcg
    18601 tcggcttttt tatttagaga aacatcattt atcagtgaaa cataacacga gaacaaaatg
    18661 attttttcgt taataaaaga aacaagtatt tgttgtttaa aataatacac gttattggca
    18721 tataggacga aatcccagta tcttctataa gtaagtcatt tcatatcgat gaaaacacgt
    18781 tatttatttt tgttttatct ataaaatggc tgaaaaatga tttttttcta aatactatat
    18841 atagtggcgt ctatcctata taccactaaa aaactacaaa atctagtctt ttattttaaa
    18901 aaaacacgtg tagaatgaag atagactttt gagttaggga gtgttttttt atgaaactta
    18961 ctgttttttg cggttcccgt tttggtaata aagaaagtta caaatttatt gcgcaaacgt
    19021 taggaaaata catggcacaa gaagaaatcg aactggttta cggtggttca tactccggga
    19081 ttatgggagt gattagtaaa acagtactgg aaaataacgg aaaagtcaca ggtatctatc
    19141 cgaatgggct ttttgaaact gaattgcctg gaaaagatgt tactacattt attcctacag
    19201 aaacgatcga tgaaagaaag gtattattat ttgaaaaggg agatgctgtc ctcgttttcc
    19261 ctggaggtct aggtacattg gaggaattct cacaacttct ttcttggata gcaatcggtc
    19321 tgacgcccga caagccaatc ggaatattga atattggcgg ttattacagc ggcttgcaac
    19381 aattattaga aacttttgca aaagaggatt ttatggataa aaatggttgg atcaagtaat
    19441 cttttccaac aatccattgg aactggtcaa tgaattacga gcagtatcta ttaaaaatca
    19501 gttattattg aaggaggcaa aataatgagt caaatatatt tagcaggacc atttttttca
    19561 gaggagcaaa tcgatcgagt aagtcgtatc gaaaaagcgt tggaggaaaa caaaacagtc
    19621 acaagtttct actctccacg tcatcaccaa gaaagcaatt atgaactatt cagcgctgga
    19681 tgggcgcagg aagtatacga aaaagatatg gaagaactga caaatgcaga atttgttgtt
    19741 gctatccttg actttgagca tcaaacaatc gatccaggaa cggcttacga attaggtgtg
    19801 gcaactatgt tgaaaaaacc gatgatcatt gtacaagaag agacggtacc tacgaacttg
    19861 atgattacac aaagtctcca tacgtattta aaatcagacc aagctgtacg tgaatatgat
    19921 tttgaaacat tgcctgttga aacttacgta ggcgagtact tatagaagag gaagggttgt
    19981 atgtcagtga ttttgatttc aggaacgatt ggtgcaggta agagcagttt gacggatatg
    20041 cttgcaaaag agattgattc taaaccattt tatgaaaatg tcgaagataa cgaagtattg
    20101 ccgctctttt attcaaatcc tgagcagtac gcttttttat tgcaaatctt ctttttgaac
    20161 aagcgatttt tagcaatgaa aaatgcttta gtgaacgatg acaatgtgct agaccgatcc
    20221 atctatgaag atagcctgtt gttccatcta aacgctgatt taggtcgggt aacggatatc
    20281 gaagtacagc agtacgatag cttattagat acgatgctaa acgagttaga tgatgttgct
    20341 ccaaaaaaac ggcctgattt gatggtccat atcaaagttt ctttggacac gatgctagaa
    20401 cgtatcaaaa aacgtgggcg tgactatgaa cagctagaaa gcgatgagac gttatatacc
    20461 tattatgaaa cgttgaacac ccgctataat caatggtatg aagattttga tgtatgtcct
    20521 aaaatacaag tggatgggga taaattcgat tttgtagaaa atccagaaga tgcaaaaaag
    20581 gtgatccgtc agatccaaga aaaattagct gaattggaag gaaagcctgc tggaaaatat
    20641 tttacggata accagcatgc aaaaggtcaa gaattaggct aaaaataaaa aaccaaacga
    20701 cgggaaagaa aaattactac gtgatgtaga ataagccttt ctatcgtttg gttattttat
    20761 attcaaatat ttgaaataaa taaatcttta ctctttgatt tgtaaactga aatgatccac
    20821 tgtttgtttt actgatgaag cttgatcaaa ggaagaagct gagtttgaag ttcataaaaa
    20881 tcagccatag tgagttttgc ttggatttgt ttatcttctt ctacttttta cataattttg
    20941 aacaggataa gattcaaatg ttttaaatga atctttgact agtttgtgac agttgtcgca
    21001 tatgctcaag cttccttggt cattaaattc tgtatggata tttttgttgt tattacatgt
    21061 acaataatca ggactatcga tcatccattt cgtctccttt acaatcatct tgcttcttta
    21121 ctatagcaga aaagaagggg agaaacaaaa gaagatccaa cagacggcac tatagactgt
    21181 agatgttgcc atcctcatcc ttcaaacggt aattaccgat ttttgtaaat atgactgttg
    21241 tttctttttt attttctttt cctgaaataa tatattcgtc atctttggta gtgatcttga
    21301 tatctgtcag cactgttttt tcttcagatg attcagaaga atcagttcct tgtgtctgat
    21361 acgtgcgtgt ccattccatt tttttatctg taaatgtcaa ctgtcccgcg gataaatcca
    21421 tatctttacg tacttttagc gtatatacac tgttggtggt atctgctttt tccgctttta
    21481 agacagaaga atcggttgtt tcgtccgagg attggtcaga gccgccaaac aaatcacagc
    21541 cagtcaatgt caaaagtccc gcagacaaaa taaatagtac gattaatttc ttcaaaatac
    21601 ttcccccatt tgataggata aagaaattat accattggaa tgtaaaactt caaacggatg
    21661 gaagagaaaa acgcacaaaa cacccacaaa tagagtaaat tttagaataa aattttaaaa
    21721 aggcaaaaaa tccgccactt tcagaaagcg ttttaaaaaa gtgttgcaat cttttaaaaa
    21781 aatgctatat taaaaatgaa ccggttcaaa tacttgttta agtataatta atagtgagag
    21841 gaagttgctc tactgaagac tttagttata tggatattga tgacaagcaa cactatgcaa
    21901 agaatgcggt catcataatc gaacaacaag aaagagaaat caaatgtaac tggcaggagt
    21961 gagaatgtat ggcatcgatc cgtgatgtgg ctaagatggc tggtgtttca gtgggaacag
    22021 tttcccgtta cttgaatggt cagcaactga aggaaaagaa catgaaaaaa atagcagaag
    22081 cgatttcagc gttggactat aaagaaaaca tcattgctaa agggttgaag aataatcgaa
    22141 gcttctctat tggacttttg ataaacggga tttccagccg ttttgggtcg gaagttgtct
    22201 cagggatcga aagagtagct gaagaaaatg ggtatagctt attattaagc gggtttgccg
    22261 atcagccaga aaaaattgaa caaaaaattg aatacctgat gaagcatgtg atcgatgggc
    22321 tgattgtctt cttatccgaa gaagagtggt ctggatttga aatgctcaca aaaatgagta
    22381 ttccagttat cgcattgaat tgtccagaag gaccagcagg tattgataca atattagtga
    22441 atgatcgaga aagtgtagca aaagtgatcg ctcatcatgg tgaacaagga cataaaaaaa
    22501 tcggatttat tgctgccact caaacagact acgtagcaag agaacgttta gcaggagcca
    22561 aagaagcggt ccaaaatgat ccatcgatcc aattagaggt ctttacagga gactattcta
    22621 gaaaaagcgg ctattacggg gcaaaagcat tattagaaaa aggggcaact gctatttttg
    22681 tcagcaatta caatatgtcg attggtgcca tcgaatgttt caatcaagaa ggtgtcagaa
    22741 tcgggaagga tattgcgttc agtcactatg attatttaga taaaaacgat ctgagtatcc
    22801 ttccgaaaat cacgatcacg ccaccaacag ataaaatcgg ggaacgagca gcagaacagc
    22861 tgttaaaacg aatcaaaaaa gaagaggaag aaaatgaaaa agtgattgtg atggaaaata
    22921 tcatacatgg aatcaactaa aacgaactaa aaaagaaaaa gaggtgcttt gatgaaaaaa
    22981 tatcaatttc cagcaaattt ttggtggggt tctgcagcaa gtggcccaca aacagaagga
    23041 aggatttcag gcgatggaaa aggagagaac atttttgatt attggtacaa aaaagaaccg
    23101 caaaagttct ttgatcaagt aggacctgaa aaaacctcgc aagtttatac gaagtatcaa
    23161 gaggatgtac agttgatgaa acagactggg cataattcat ttcgaacatc tatccaatgg
    23221 agccgtttga tacctgatga atctggaaca atcaacgaag aagccgttcg tttctatcat
    23281 cgttattttg atgagttgat tgaaaacggt atcgaaccct ttgtgaatct gtatcatttc
    23341 gatatgccga tgtatctgca agaaaaaggc ggatggttga accgagaaac ggttgaagcc
    23401 tatgaaaaat atgccaaaag ttgttttgaa ttgtttggtt cgaaagtaaa aaaatggttt
    23461 actcataatg aaccaatcgt tccagtcgaa gcaggttatt tatatcgatg gcattatcca
    23521 gaagagtcag atatgaaaaa agcgatccaa gttggctatc atgaagcgtt ggcttcagcg
    23581 ttagccatca aatcttacca tgaaatggag caaggtggtg ttattggtat catcttgaat
    23641 ctgacaccaa gctatccaag agacgaatca aaccctgaag atgttaaagc agcgcaaata
    23701 gcagatgcgt tcttcaatcg ttcattcctt gatccagctg taaaaggcga gtttccaagt
    23761 gaattgattg agatagtaaa agaacttgat ctcatgcctg tgatccatgc agaggatttg
    23821 acggttatca aagaaaatac agtagatctt ttaggtatca attattacca gccacgccgg
    23881 gtaaaagcaa aagaaacacc aatcgatact aaaaacggtc caatgcctga ggattatttc
    23941 gacaattatg aaatgcctgg tcgtaaaatg aatccttatc gcggatggga gatctatgaa
    24001 caaggaatct atgatatttt gatcaatgta cgagatcatt atggaaatat cgactgcttt
    24061 atttctgaaa acggtatggg tgtagaaaac gaagcacgat ttatcaaaga agatggcatg
    24121 atccacgatg attatcggat cgaatttgtc caatctcatc tgaaatatgt ccatcaagcc
    24181 attcaagaag gtgccaattg tttaggctat catatgtgga catgtatgga taactggtcg
    24241 tggaccaatg catataagaa tcgatatggc tttatttcag tagacttagc acaagatgga
    24301 aaaagaacaa tcaaaaaatc gggtcattgg ttcaaagacg tatcagataa aaacggattt
    24361 gaggcttaag aaagaatagt tgagagcaaa tgcgtgaaat tctccattct taccattttg
    24421 tgaaaaagaa gttcaattat ttacgatctt tttcaacttt tttgtctttc tatttcattt
    24481 tttgtatata atagaatagt caaaattaaa taggagtgga ggaaagcatg aggaaaaaaa
    24541 gaccgtttct tctcttttgg ttattcggtt ttttcttact gcttatttta tccgcagcat
    24601 tttcaggaaa aatgacagca gatcttgctc agaaagaaac agaaaaaatc gtagcaagca
    24661 aagaatcagt agataatttc aaaaaagaat tctcaactta ttggtgggcg gagtataaaa
    24721 taactgccaa ttacgaaaaa ggcgaattgg acatttattt gccaaaagga aaagaagaaa
    24781 atcaatttat taatatcgat gacgtactta gtgcgatttc tctcagacaa tatcaattta
    24841 aagacaagca tttgaccgtt ttgcttttga ctcatgatgg cgacaaagta gcccgtttga
    24901 aaaatggaaa agtgatcacc gcttattctg aaaaagtaga caaaaaagtg gtcgccgatc
    24961 gtttagcgga atggaaaaag acaaatgaac aagtcaagaa aacaactgtc tcttctcaaa
    25021 aaaacgagtc gacaggaaca agtccttcag attctggcag tggacaaacg ataacgggaa
    25081 gtgaagaaaa tcaagcctca attattacga atgagtcatc acaaggacaa tatcaatcct
    25141 atggacaatg aaaaacagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
    25201 aacggcaaaa tagttcctca tattttgccg ttttttgagc taatacc
//
LOCUS       DAFFSV010000029        25121 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFFSV010000029 DAFFSV010000000
VERSION     DAFFSV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.029"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            12..84
                     /locus_tag="LR110_001080"
     tRNA            12..84
                     /locus_tag="LR110_001080"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="LR110_001081"
     tRNA            88..160
                     /locus_tag="LR110_001081"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="LR110_001082"
     tRNA            168..249
                     /locus_tag="LR110_001082"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="LR110_001083"
     tRNA            267..339
                     /locus_tag="LR110_001083"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="LR110_001084"
     tRNA            351..422
                     /locus_tag="LR110_001084"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="LR110_001085"
     tRNA            439..524
                     /locus_tag="LR110_001085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="LR110_001086"
     tRNA            535..608
                     /locus_tag="LR110_001086"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="LR110_001087"
     tRNA            618..691
                     /locus_tag="LR110_001087"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="LR110_001088"
     tRNA            711..783
                     /locus_tag="LR110_001088"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="LR110_001089"
     tRNA            811..884
                     /locus_tag="LR110_001089"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="LR110_001090"
     tRNA            898..971
                     /locus_tag="LR110_001090"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="LR110_001091"
     tRNA            995..1084
                     /locus_tag="LR110_001091"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="LR110_001092"
     tRNA            1097..1170
                     /locus_tag="LR110_001092"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="LR110_001093"
     tRNA            1189..1264
                     /locus_tag="LR110_001093"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="LR110_001094"
     tRNA            1266..1336
                     /locus_tag="LR110_001094"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="LR110_001095"
     tRNA            1350..1423
                     /locus_tag="LR110_001095"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="LR110_001096"
     tRNA            1448..1535
                     /locus_tag="LR110_001096"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="LR110_001097"
     tRNA            1557..1628
                     /locus_tag="LR110_001097"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="LR110_001098"
     tRNA            1651..1740
                     /locus_tag="LR110_001098"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="LR110_001099"
     tRNA            1753..1826
                     /locus_tag="LR110_001099"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="LR110_001100"
     tRNA            1845..1917
                     /locus_tag="LR110_001100"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="LR110_001101"
     tRNA            1921..1993
                     /locus_tag="LR110_001101"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="LR110_001102"
     tRNA            1998..2068
                     /locus_tag="LR110_001102"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="LR110_001103"
     tRNA            2081..2154
                     /locus_tag="LR110_001103"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="LR110_001104"
     tRNA            2166..2237
                     /locus_tag="LR110_001104"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="LR110_001105"
     CDS             2503..3696
                     /locus_tag="LR110_001105"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBL4822077.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="LR110_001106"
     CDS             3826..5298
                     /locus_tag="LR110_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4822078.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="LR110_001107"
     CDS             5381..6169
                     /locus_tag="LR110_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBL4822079.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="LR110_001108"
     CDS             complement(6236..6904)
                     /locus_tag="LR110_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBL4822080.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="LR110_001109"
     CDS             7019..8731
                     /locus_tag="LR110_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822081.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="LR110_001110"
     CDS             8734..10503
                     /locus_tag="LR110_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822082.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="LR110_001111"
     CDS             complement(10708..11274)
                     /locus_tag="LR110_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL4822083.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="LR110_001112"
     CDS             complement(11274..12230)
                     /locus_tag="LR110_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBL4822084.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="LR110_001113"
     CDS             complement(12230..12886)
                     /locus_tag="LR110_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL4822085.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="LR110_001114"
     CDS             13256..15274
                     /locus_tag="LR110_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL4822086.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /locus_tag="LR110_001115"
     CDS             15701..18115
                     /locus_tag="LR110_001115"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBL4822087.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="LR110_001116"
     CDS             18463..20016
                     /locus_tag="LR110_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL4822088.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="LR110_001117"
     CDS             complement(20397..22181)
                     /locus_tag="LR110_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822089.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="LR110_001118"
     CDS             complement(22196..23917)
                     /locus_tag="LR110_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822090.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="LR110_001119"
     CDS             24017..24778
                     /locus_tag="LR110_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL4822091.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IRERKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7760 a         4621 c         5357 g         7383 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag caaagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttgtaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcgaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacact t
//
LOCUS       DAFFSV010000030        24549 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFFSV010000030 DAFFSV010000000
VERSION     DAFFSV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.030"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            16..255
                     /locus_tag="LR110_001120"
     CDS             16..255
                     /locus_tag="LR110_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822092.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            382..1071
                     /locus_tag="LR110_001121"
     CDS             382..1071
                     /locus_tag="LR110_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBL4822093.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            1077..2243
                     /locus_tag="LR110_001122"
     CDS             1077..2243
                     /locus_tag="LR110_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBL4822094.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            2325..2996
                     /locus_tag="LR110_001123"
     CDS             2325..2996
                     /locus_tag="LR110_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL4822095.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIKMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAKMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3049..5025
                     /locus_tag="LR110_001124"
     CDS             3049..5025
                     /locus_tag="LR110_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL4822096.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            5038..5790
                     /locus_tag="LR110_001125"
     CDS             5038..5790
                     /locus_tag="LR110_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL4822097.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            5806..6567
                     /locus_tag="LR110_001126"
     CDS             5806..6567
                     /locus_tag="LR110_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822098.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            6580..6840
                     /locus_tag="LR110_001127"
     CDS             6580..6840
                     /locus_tag="LR110_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822099.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            6837..8927
                     /locus_tag="LR110_001128"
     CDS             6837..8927
                     /locus_tag="LR110_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL4822100.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(8963..11020)
                     /locus_tag="LR110_001129"
     CDS             complement(8963..11020)
                     /locus_tag="LR110_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBL4822101.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(11165..11356)
                     /locus_tag="LR110_001130"
     CDS             complement(11165..11356)
                     /locus_tag="LR110_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822102.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            11544..11999
                     /locus_tag="LR110_001131"
     CDS             11544..11999
                     /locus_tag="LR110_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822103.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            11996..12238
                     /locus_tag="LR110_001132"
     CDS             11996..12238
                     /locus_tag="LR110_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBL4822104.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            12256..12558
                     /locus_tag="LR110_001133"
     CDS             12256..12558
                     /locus_tag="LR110_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822105.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            12627..14681
                     /locus_tag="LR110_001134"
     CDS             12627..14681
                     /locus_tag="LR110_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822106.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            14681..17293
                     /locus_tag="LR110_001135"
     CDS             14681..17293
                     /locus_tag="LR110_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBL4822107.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            17290..19668
                     /locus_tag="LR110_001136"
     CDS             17290..19668
                     /locus_tag="LR110_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822108.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            19729..20271
                     /locus_tag="LR110_001137"
     CDS             19729..20271
                     /locus_tag="LR110_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822109.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            20349..20681
                     /locus_tag="LR110_001138"
     CDS             20349..20681
                     /locus_tag="LR110_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822110.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            20682..21275
                     /locus_tag="LR110_001139"
     CDS             20682..21275
                     /locus_tag="LR110_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822111.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            21297..23321
                     /locus_tag="LR110_001140"
     CDS             21297..23321
                     /locus_tag="LR110_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBL4822112.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            23321..24520
                     /locus_tag="LR110_001141"
     CDS             23321..24520
                     /locus_tag="LR110_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL4822113.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
BASE COUNT         8622 a         3892 c         5007 g         7028 t
ORIGIN      
        1 aaggtaggaa aactcatgaa ttatgaagaa atcgaaaaca gaaaaaaagt gtcgaaagaa
       61 atggaagaaa agttgctaaa aatgatgaaa caaaaacacc ttaaaagatt atctgtcatg
      121 caatatatca atgatatgaa gataacagga aaagagaaag cttgtttgtt aggttcaatg
      181 aaaaactttg aacagttgag gcgaacttat gtaaaaacag gttccaattg ccaactatta
      241 ttagaagttt cttgacgatt atgatatgaa aatgatcaat tatgatgtgg cattgttatt
      301 gaataaaaag aatagtaaaa tttaacatgg tttatcttgg ttagatgtag gtaaattaat
      361 tcatatatag aaggtagata gatgaataaa aacaataaaa ctttatttaa tccttacgtg
      421 ggcgtaatca ttgtttttct tctttttagt tattttgctt atcaaggtta tcaaaaggac
      481 aaaatcgctg aaaaagtaga tggtgtaaca aaaacaatcc atattcagga acaggctaaa
      541 acctatcaag ataatttaac cagtttaacg gatatttcta agagacaact gattactaat
      601 caaaaacagg cattagaaaa ggatctcaaa ggtgaaattg ctggaaaact tgttttttct
      661 tctacaggat ttattctccc tgtttttcaa ggagcgaata gcgacacgct tagtttagga
      721 gtagcttcta cttattatct agatactgaa atgggaaatg gaaattatat tcttgctggt
      781 tataacgtag atacaccaag cgttttatta tccgatatga ctgatttatc tgaaggagaa
      841 gaaattgatc taatcgatgc tcattacaag tatcagtatg aagtctccaa aagaggaaaa
      901 atttctgatt acagccaggt agttcaatct aaggaaaaca attccttttt atccttgcca
      961 aaagcaggta aaaaaccact actcacactg ttagcctttc aagcaaataa tatgaaaaag
     1021 tgcgatgtga tccagtgtga attagtcaat gtagaaaaaa tcaataaata agggaagtga
     1081 gtcacattca agaacttgta gaaaaattga tcaataaaat gagtgccatt tttctgttac
     1141 tgttggtatc cattctatat ttaatatgga cggtggggtt aaatcctcct acgatattat
     1201 ttatttatga taatgctgca cagttagtga cgtttttcgt aggctgtttg tttcttttga
     1261 atctgtacaa ggtaaagaga aacgatctat tcttcattgc agcaggcatt cttttttacc
     1321 ttttcttttc tttttttaga agctaccgag gagatacttt ctatggtgat ctgatcgttc
     1381 cgacaattat tgctattatt ttgattgtaa aatggacgaa atttactgta attgatcgta
     1441 gcttgtatct tgtagtattc tatgtctttt tactcgtaac ggtttatcga gtatttactg
     1501 agataccagt accagaagga caaagtattt ggaatccatc gaacaaatta tcagatattt
     1561 ggatcaatac aaatacgata ggtagttctt taatgacatt agcccttctt atcacaggat
     1621 ttgctagttc atttggaaaa tggtacatgc gattgctatc attaccagcg ttgattcttg
     1681 ctggggcaac gatttgggtt tgccaatcga aagcagcatt atatgcgctt gttatcttca
     1741 ttatcttaga aattttacct aagaaggttt ttcgaaatac acacttgtcc ttttggatgt
     1801 atgcagcagt ttcggttcta gctttaccga tttcttattt tgctgcaatg tcagacaaag
     1861 ttcacttatt tacaggacga gaagaaattt ggcgcaaatt ctatgaaaca ttaggcgaaa
     1921 aaacaggaca aatatttgta gggatgaaac ctttcttttt ccatagagga aatcagattc
     1981 taggtaatca taatacgtat cacgcactcg tcaatcacta tggattgatt ggttttgttc
     2041 tatcgattat tcttttgctt agtttcttat ggttgctcgt aagaaaacgt gagctgacaa
     2101 atggacaact aacattcttg tgggcttttt tagcgatcat gtgtcaatcg ttcatggaag
     2161 aaacattaac gacaatcagt tgggtgccaa ttgtattctt aatgttagca atggctacac
     2221 accaatataa aaccaccaga taaatgaaat agaatagtaa taagagaaaa cactttttcg
     2281 ttgtgaaaaa gtgttttttg ttttgtctga aaggaggcag atggatgaaa aaatggttat
     2341 ttggtttttt aggagtcgct ttaatcgtcg tttgcagtgt gtttggctat gttagctacc
     2401 aaaaacatga aggagaggtg tttaagcaaa atatagaaaa aaagatgccc gtagatcaaa
     2461 tcaatgcaca cgcaaagagt tataaggagg atgcaacgaa cgtcaataat gatatgtctt
     2521 tggggcaaat gttgtccata caaaaagaag caatcaaaat gggcgtcaat aaacaagtat
     2581 ttgcacaaat tcagattcct gcacttggtt tagcattgcc aatttttaaa ggggcaaatc
     2641 aatacacttt gagtcttgga gcggctactt atttttatga agatgccaaa atgggcaaag
     2701 gaaactatgt cctagcaggt cataacatgg aaatgccagg tgttttattt tctgatattc
     2761 aaaagttgtc attaggggag gtgatggact tagttagtaa cgatggcgtc tatcgttaca
     2821 aggtaacgcg taaatttatc gtacctgagt actttaaatt aatcgatggc gttcctgaag
     2881 agaactcttt cttgtcttta ccaaagaaag gagagaaacc cttattaacg ttgtttactt
     2941 gtgtttatac gtcacaagga aaagaaagat acgtggtaca gggagagcta caataacaag
     3001 aatttaatca acgcgagtcg ttgaaatatg aaatgaggag agtaaaaaat ggcaataaat
     3061 cggtatgtaa aaaaagctgg aatgattgtt ggaatgttag gaatcattgc aggctgcttt
     3121 caggggtatc gagcttatgc agaagatgtc acgcaaaaaa cgccaccaga aagggtcaat
     3181 atcacggttc ataaattaat gtacgatcaa agtacgcaat tgaacgttga tattgacgga
     3241 atcaaaaatg atggttatac gcacgatcaa tatcctacag gtgtaacgaa atacaacaag
     3301 gcagattatg gtgatgttga gtttacgtta ggaaatatta cggatcaagt attaccgact
     3361 gaagattctg atctaaccaa cgcgaaagtg gacgaaatcg ttaaagatgt agaagacaag
     3421 ggatctaaca gtgagtacgt taagaatgca accaataaaa ttacttcagc tgtagatgaa
     3481 aatggtgaga tcacttttgc tgatcaacca gcctatgtca attccaaagg gaatgtatat
     3541 gtggtttatg aaagtaaatc cgctgctgga ttagtcacac aaaaagcaaa accaatggtt
     3601 gtgattgcgc caatgacaga caatacagga tcaggatttt taaaagatat tcatttgtat
     3661 ccaaagaaca ttgtttctaa gttgagtttt gaattaacga aatttggtga cgatggcaca
     3721 gctcaaagca aacaaacacc tttaaaaggg gcaaaatttg agttatacaa gggcgaacct
     3781 ggaaaaggaa caaaattagg agagcttgta tcggatgatc aaggaaagct gactgcaact
     3841 gatttgacgt taggaaaata ctactttgtg gaagttcctt cagaagttgt agtgggatca
     3901 gatcaagaac caacagcaga ccaatatttg ttaggcgcag acgctcgcaa cgatgcgcac
     3961 aataagttaa cctttgaaat tacgaatgac ggcgtgacaa gtgatttaaa agcttcctac
     4021 gtgaactata aagcaccagt gatcgataaa acagtgacaa atggaacagg agaagaacat
     4081 tctttccaaa ttggtgacgc agtgaattat caaggtacaa ttcatatccc tacagatatc
     4141 gcaggtggaa cagatgggat tacagtaaat ggtgtaaaaa gtgaaacttc tccttacagt
     4201 gtatttaaat ggggcgacac agcaggtaaa ggactttctt atgttgctga aaaagcagat
     4261 attaaagtaa caaataatga tggttcagtc gtattaaaag aaaatactga ctataaaatc
     4321 gaaaattctg atagtggttt tgtgatcgat tttatcgtaa ataatggaca agtaagtgat
     4381 acagttgcga atttacatgg tcaagatcta aaaatgactt ataacatgtt tgtgaatgat
     4441 agtgctgaaa tcgcaaatcc attaacaaat agcgtggatt tcgtttataa caataatcca
     4501 tttaatcagg aagaacacca cgaaaaaacg aaagctgacg ttgtgactta tggcgcaaaa
     4561 ttcttaaaag tcgatagcgg cctatttgga acaggtatca aagcaacacc gcttgaaggc
     4621 gcagaatttg cagctaagaa tgcagaaggt aagtactacg gtggacttac tgatacagat
     4681 aaagatggtg tcaaagaagc agtttgggta gacgatgtag caaatgcagc aatcctaaaa
     4741 tcagataaag agggtcattt tgaaatcact ggtttaactg aaggagatta ctcactggaa
     4801 gaaacaaaag ctcctgaaaa ctatcaaaaa ttaacaaaag agatctcttt caaagtagac
     4861 aaagattcat acaaagaaga aaaccgaatc acgatcaaaa ataatcaaaa agcttccgta
     4921 ccaatgactg gtagcaacgg tttccaatct tacgtattga tctcttgctt gttgcttggg
     4981 gcaggcgcaa tttctgcagt tgtttacttt aagaagaaag cctagaagga tcagctaatg
     5041 attatcagac accctaagaa aaagcgaatc atgggtaaat ggatcattgc tttctggtta
     5101 ctttcagctg ttggtgtctt acttttgatg cctgctgaag cgagcgtggc gaaataccaa
     5161 caaaatcagc agattgccgc aatcgatcga acaggaacgg cagcagaaac ggattcttct
     5221 ttggatgttg ctaagattga gttaggcgat cctgttggta ttcttacaat tccaagtatt
     5281 tctttaaaac taccaattta tgacggaact tccgacaaaa ttttagagaa tggtgtaggg
     5341 attacggaag gaactggcga tattacagga ggaaacggta aaaatccttt aatcgcaggg
     5401 cacagtggac tttataaaga taatcttttc gatgatttgc cttcagttaa aaagggagaa
     5461 aagttttata tcaaagttga tggtgagcag cacgcttatc aaatcgatcg gattgaggaa
     5521 gtgcaaaaag acgaactaca aagaaacttt gtcacttact tagaaccaaa tcccaatgaa
     5581 gatcgagtaa cgctaatgac atgtacgcct aaggggatca acacacaccg tttcctagtg
     5641 tatgggaagc gagtaacctt tacaaaatca gaattaaaag atgaagaaaa taagaaacaa
     5701 aaattatctt ggaaatggct tttaggaagt actgtttttc tttcagtcat gatcattggt
     5761 tcgctatttg tttataaaaa gaaaaaataa aggagggtaa ccaacatgag aaatagaaaa
     5821 tggctatttg tttgtgcagc cgctcttttt tctttcactg cctacacaca gttgacgttt
     5881 gcagacagac aagaaagcac ggatagcaca gaacaaacaa tccaaacgtc agatagcagc
     5941 acgattcaaa gcagcgatga aaccgtcgat tcgtcaacag ttgattcttc agaagaacct
     6001 aaaattgctg aagacgatca aatagtcaaa cagaccattc acgttcaaaa aattatttct
     6061 tccgaacaat tagacaacca ggggcaaaag cttgaaaaac aagaaggagt aaatggcgcg
     6121 aagttttctg tttatgatgt atcggacatt ttacaaaaga tggatgttaa ggatctcact
     6181 actgaccaaa ttgaatccca attaaaagat cgagtgaaaa aactatccag tgatcaactg
     6241 aagctaattt ctaatggaga aacaaagaca attgatcaac aaacaggtgt ctttgaattc
     6301 tcggtagaag tacaggcaaa tcagaaacaa gcttattata ttgtcaatga atcaagtcct
     6361 gaaaacattt ctaatagcga ggatattttg ctattaacac ctgtttctga taaaaatggt
     6421 gaattcttaa aggatgtgtg gatttatcct aaatcagaag cttcgcaacc taaagaagaa
     6481 gtaaaaaaaa tcgtatctac tggcgtaaag aaaaacttct ttgagaattg ttgggatttt
     6541 gttactcact tgttttctag agattaagga agaaataaaa tgaaactgat caaattatta
     6601 ttattcaatt tttcttgttt aaccgcagtc tttttgtact gcggtttatt attttctaaa
     6661 ctatctattt tgccaagcta ccaactatac ctaatgagga ttttaaacat accagctgct
     6721 tccatgccgc tagatattat ggaaaagctc attaggaatc cattatttac tacgatcaat
     6781 ttgatcgtta ttgtcggaat ttcactagtc atttataaaa aagaggggat gaagggatga
     6841 attttaaaca ctcgtggaca agacggttaa aatatgtttt tcccataatg atgatgttag
     6901 gtacattgtt tgggttaaaa acagccaatg tctttgctga aaaagttatt gtcgatccgt
     6961 caaatccaat ccagaacgta aagaataatt ttattattcc aaaagatgtt cctaatgcaa
     7021 aaacaaatat tactgtaaac ggagcgtcaa aagtttacaa atatcatgtt gatgccaaca
     7081 aaggtggttt tacagacgac tacgtaggat tagtggaggg aaacaaaaat atacgctatc
     7141 cttctttcca aaaagaatac ggtgaaacaa accttgtttt agaaggcgta acaaccaaca
     7201 ccaaagtcaa tattgactat ggaaaaatcg gtacgtataa cgggaaaaaa gtcaatatca
     7261 agctagttct atctaatatt cacttatact cggatacgtt gccgtggaat ctactagata
     7321 acaactatac aaaaactcat tttcgagacg atggctataa aaatacgaat ggtgcaatga
     7381 gtaaatctaa aaaaagaaca gtactttgga tctcagacaa tcttttctca ggaattgttt
     7441 accattcaac acaaatgaat gtgcagctag tagccacata tgaggacggt agccccgttc
     7501 aattttcagg agatacattt atcagtttta actctttgaa ccctgcagga ggaaaaagca
     7561 cggatcttaa aggagaatat gcccactatg acaaaatgaa tacgacagat tggtatgtca
     7621 gaaaagatac ggttctttca gaatttaaat ctttctacaa caatctgaac gtggtaggtg
     7681 gccacccagg aggaagctct aagctgacac aagcggataa tgatttcaat aatttgcatg
     7741 ataaattagg agatccgaag tttggtcaag gtactgtgtc ctttaaaata tctgaagcaa
     7801 atccgacgtt tgtgattggt tcttctaatg ttcaaacctg gtttacttta tcttctgcga
     7861 caatctttag tgttgttcct gatcaacctg aaaaaacagg ggttgataaa aatggaaata
     7921 acgtgaacga taaaatgttg caagtaggag acacgattca atatcgaatc aaacagaagg
     7981 tgaatcgatt aggagtggat ttactagcgg tttacgatcg atttgagtta atcgataatt
     8041 taccaaaaga agtaaattac gtcgatgcac acgttgaaag tggtacaaat aaaaaattcg
     8101 atgtatctgg tgaagtgacc tatgacaaaa caaaacacca ggtaaaatat gcagcgaaag
     8161 ctgatacatt aaaaaatcga atgaagtata acggcgaaac ttacgaatta gtgatcaatg
     8221 taaaagtcaa tgaattggcc aatcaaaata gtgtagccaa aaatcaggga acgtcgatca
     8281 ttaataaagt agaaaaggat acaaatatca ttaccgttta tttccctaaa attccagtga
     8341 aagaagtaca acaaaatgga aaagatgtaa acggacgaaa tgacggtaag aagggtacgc
     8401 cgacagctcc tttaaacgca ggatctgaag tacaatatct agtcacacaa aaatggcaca
     8461 caaaaggcgt agacgctgcg tcagatcact acaaacaatt tagtattcaa gatccaattg
     8521 aagctcgttt gacatataaa gaaggttctg cgcaagtaat agataagagt aaaggaaaag
     8581 atattacttc tgaaggcaca ctaacctatg atagcaattc aagaacgctg aagtgggaag
     8641 cttccgctga cttcttaaat aacaatcttt tagatggacg agaaattcag ctgatattta
     8701 cagctaaaac tccattacaa tcagaaaaaa atattgataa ccaagccgta gtagcagtag
     8761 ataatgtttc taataaaaca aatgttgtga caattggggt tgatcctaac ttgccacaag
     8821 ttattgttcc taaaacaggt tctacacatt tagtaacgat tttagcggtc agcttactat
     8881 tacttgtgtt agcaactttt ggctacgcag ttctgaagtt caattaaaca aaaaaacaag
     8941 gctgaaaatc agccttgttt ttctatttga aaatgggatt ggcactaata aatacgttgt
     9001 ctgaagaaag tgatccgtct gcagaaactt cataagcatg ggataaacca acgatacttt
     9061 gattgttgta aagcaagttt gcgttatgag cagcgctatc tttccacata accatagcat
     9121 ttactgctcc tgtatctgaa aggatctcgt caacgtaaag gtagaaacta ttgatatttt
     9181 ctcctttagc accgtagcag cctaagcttt ctaagagatc ttggaaagta ccgccgccag
     9241 gtcggtaatg actgaagctt acttgtaatt cttttgcacg aatatctgca gcttgttgaa
     9301 cgatatcatt gaattgcatt ggggcaacgc cattttgttg acggtagcta tttaaccaag
     9361 caagttcttt ttgacgcaca tagttgttta aatcacttag tgagatattt ttgtatttga
     9421 aagtgatcgt tcgattttgg tcaacattat tgattacacg ttgtccattt ttatcaacgg
     9481 taacagatga atcgtcattt ctacacaaga agtagttttg atcggtcata tttttccaag
     9541 aagtagagaa actatctccc tcgtaaaccg tttgtgtttc agtagataaa acagcgtttg
     9601 tgtcaacgtt tatatggttc acagtgacgt tgtattgatg agtataggca aaattgattc
     9661 cttgattact tgtaacgttg tttaaagtca cagtgttgct agagctttct ctaggatcaa
     9721 gaacaaaatt agtgaacata ggtgcttcaa ttgtgatgct ttgtccataa acgacgggat
     9781 aactcttact gtaattatag agttcttgga tatttggatc agtatccgag actttatttg
     9841 gacgagcttt cacttcaact agaaagactt catcatagta aaaagtaaat gtggtatttg
     9901 tgttaatcgt ttgtgtttgt gtttcattcc catttacttg atactgtggt gcttcaaagt
     9961 tagtcaagtc ggttctagct tttgcagtgt aacttgtacc agcttttact gagacatgtt
    10021 cttcttttag cagcgttcca gtttgtcgat tcatatgacg gacagttacc gcatattctg
    10081 aaggtttagg tttgtgttgt aaccctttaa atgcagaatc aagactattt ttagcattat
    10141 ttacttgggt ttgagaagct ttagaatcgt ctagtacttt tttagcattg gataaagcag
    10201 tctgaaaatt attccaagat ccttctgtaa aatctccttt agcagttgat tttacttggt
    10261 tgtacaaggc ttgtaattcc gttttgttca cagtaggagc tggttttttc tttaatccct
    10321 tgtaagcaga atctaatgtg tttttggcac tatccacttg tttttgagtc gcttttgagt
    10381 catttaacac ttttttagca ttgtttaaag cggtttggaa tgcgttccag gtattgtcgg
    10441 tatagtcacc ttttttagtt cccttaacgc tcgtataaag agcttctaaa gctgatttat
    10501 tcactttggg tttagaggtg tcagcaccat accaaccaat accttcgtat ttccagccat
    10561 agtctttggt taaccattgt acttctttcc aacttgcagt gtagtgatgc gaaccagagg
    10621 taacacctgg aaggaaaagg cggtagatcg gtttattatt tttttcggca gaacgccagg
    10681 caggcccttc ataagtccaa ccgtaatttt tggtcaacca tttcacttca tcccaactag
    10741 ctgtgtaatg gtgatcacga aggcctttat tgtataaacg atagacaagg tggccaccgc
    10801 tagtcggtgc ttcccaagcg gggccttcat aattacccca cttaattttc actaggtgat
    10861 cacgttcttt tgtgctagag gtgtaaaaat gttcatggtt atttggatta taaaggcgat
    10921 acatgacgtt agttgaagca gcgtcagcag atccagcaag agcaactgtg gcagcaaatg
    10981 ttccaatagc tataacagtt tttgggttta ataatttcat gttttcaact ccctattaaa
    11041 tgattaaata ttttgtttac attcataata taccacggta taccgaggta gtcaacggta
    11101 taccgtggaa attttgaaat aaaaaagccg ccctatgaag agtgtaagga tggctgttta
    11161 tatgctatga agaaatggat tcggcgtatt tggttagttt ggcagcatta tcaaatcgca
    11221 ttttttcaat cgcacttttt ttctttatta aatccgcaat tgttgattct gcgactgaag
    11281 aatgtttgct aatttcatat gctgtcttgc cagtttcaaa tagtaaccat tcgattttct
    11341 ctttatcagc gtacattgca atgctccctc ttgatttttt ctttattata gctctttttt
    11401 gtaataaatc acaatatcaa gaggaaaagt ttctatagaa aggtgggaaa ttatgagtat
    11461 ccgttccccg tgtatcaaaa tgaggtttgt tgctactttc gttatttaat atactaaaat
    11521 aaaacaacga aagaaggaat agaatgaaaa agttaataat ggtatttact tcgttgattg
    11581 ccatgattgt tattttaggt ggatgtggta gtgaaaaaaa taagcaagat agtcaagata
    11641 atggcgtgtt aaaaccagga acagtgtgga aagaagaagt aggcggactt gttcataatt
    11701 taaaaattat agatgataca acatgggaat attccgaaag tgtttggcat cctgaacaag
    11761 tacaaataac agttgagaag ctaaaagact ataaaggatt agaaagatat aaggtaatcg
    11821 attcaggtgg agtagattta ttcataggta aaacagcctt aatagttccc tataacaaag
    11881 acggtttaga aacgtttaaa tttattccaa caaacgaaaa agaacatcga gataaaagtg
    11941 aaatattaaa agaatacagt actaaatctg gatacaagtt acaaaagact agcgagtgat
    12001 tgctagtctt tttatttatc ctgaaggagg aatcgcagtg gcaaaagcta aaacgccatt
    12061 tgaaaaagcc gcgtctaaaa tcttagaacg tcgcggtgta tctgaaacac agtggaaaca
    12121 agaagtcctt gaaaaagcac aatttgacct attacgggga aaagacaagg acttagaagg
    12181 atatatctat caacgtgaac gtgaaaagct tgttttcgat gaaattatta aacactaaaa
    12241 ggagcgaaca attttatgtt tgaatcaatt acaatgaatt ttttaacaca accgaaagta
    12301 gtagctgcgg ttgatatttt tagagcatta caaggtgcag caactagttt aaaaaacaat
    12361 ctgatgattt ttgcggctgt agctgccgtt gcctccattg ttcttggggg gattttcttc
    12421 ttctttggcc gaagaggagc tgaaaacggg aagatgcacg taggttatgt attgatgggg
    12481 gtaggagtta tattagctgc cgcctctatt gtggtttcgt tatttggaat gtttggacaa
    12541 accccacagg gactataaaa aataaataga aggagtaaaa agtacgtggc tactttttac
    12601 tcctttttta tgaaaggaga atactcatgt actttacgat ttcaggcaat accaaaaaag
    12661 ataaagagtt aataaaaaaa gcaacgaaga aagtgggaaa gcgtactgcc tttttaccga
    12721 aaacaattaa atattctgtc attgaaacgg aagaacagct acatgtgtta ggacaagtgt
    12781 tagaacaaga gaatcaatct tctgttaaat tgactctcac gttacatgat aaagaaggaa
    12841 aaatagaaaa ggctggcaca gaatacgttt ttggcccact ggattatcaa atttcagaag
    12901 gtttttatcc tacattgatg gatttgatcc gccttgatgt gtttaaggaa acggatgaaa
    12961 ccatgagtgt ggaagagtct tatcgaaacg aaaaactgct acaagtttta gaagaagagt
    13021 tagagagcga ccaaagaaaa gtgagtcaag tgagtgaagt cgaagtaaat gatgaagtac
    13081 catgggaacg accaatcttt ccaaagaagg agtcaaaaga aaaagagaca gtctatgttg
    13141 acgaacaagc tgatattccg actcagaaat cgatatttga ggaagagaac gatgtaatgg
    13201 gtcaattgga ggtagatccc tttgaagaaa caatggagga tgaagagacg gccgaagtag
    13261 aagttgatct agttgaagct aatgaagagc caacatggac tgaaggggat tctcaggaat
    13321 atattgaacc acttatcagt gaagaagtag agaaaaacga aacagagata caaaaggcaa
    13381 ctgtatttga ttatattttg gatcaatatc cgccagagca tgaatggatg aaagaaaaaa
    13441 ataaattatt tttaaatgag ctttattcga aatatcaatt accccaaacc tatcaagcat
    13501 ttttagagag taaagaagaa ttaatctcac agggtaaagg cacactttta gaacagttag
    13561 agtctgttca atcaacaaat tggcatgagc ttgcacaaga agatttatta gccacttttg
    13621 aagaacgtca acaagaaaca gatcaagaaa taaatgagta tgcgacggaa cagtcatcta
    13681 attgggagag aacaaaagag gaactaaata aagaagaaca acgagtgatt gaagaagaag
    13741 tacaaaaaat taaagagcga tacgaaaaga aacgcgacaa tgcgtattcc ttgtgtatag
    13801 agaaaattaa taactttact aaaactaatc aagaagcttt atcgaaagaa aaagaaaact
    13861 tgttaaaaga acgtataaaa gaacgaaaag aagagttcta ccgaggttta cgtacagata
    13921 agctaaagat tagcaaagaa ttaaatgaac aactgaataa actttatcaa acaacgactg
    13981 aagttcttat ggaaagacac gggcagatcc agcaagaatt acaagcacaa attccaaaat
    14041 ggaaaaaaga gtatgaaatt caacaacaag aattgaaaga acaagaagaa aaacaacgac
    14101 aagaagaaaa agagcgcgaa caattagaac taaaaaaacg tgagcagcat cttgcagaac
    14161 aaaaagtggc gatagaaaaa caacggttag agcaagaaaa ggaaaaagaa gcagctgctc
    14221 gtaaggaacg tcaagaacaa atggatacat tgcgacaagc acagttgaat tatctagcac
    14281 aacctcaacc agtagctgct gttcaaacgc cagtgaacaa ccaagcaaat ttaactcctt
    14341 caccacagcg ttcctttaac ggatggatgg tgggatgtat tgcaagttta agcttgcttc
    14401 taggtggagg aacagtattt gcttttaatc atatcaatca atcttcacag gcagattctg
    14461 tagccacaac ggcgtcaatc aaggaagaag cccaggaaca agcaagaaaa gagtatgaac
    14521 aacaattgga agaattattg aatcgtacca atcagtcaac agcagagtca agtatggaaa
    14581 atagcaaaga gagtgatcct agtaaggcta aagaaggatc aagcgattca actgtacact
    14641 caaacacttc cgaaagttca gaatcaaatg aggtgaaata atgcgtatta gagaaacaac
    14701 gagacgaaaa gacaaacgat tcaatttaga aaagaatagg tatctttctc atttctatcg
    14761 ctatactcct tcaggagaag agctaccaga aaaacatgac aaacgtattt ctcaaaagat
    14821 tttttatagt ggcttagctt atcttccttt aaactatgtt gttacaagaa taccagaagc
    14881 atttcatcga tttgagatta ttccaggaag tttacaagga gtgaatattt ttcagttaca
    14941 agaaatgccg cagttattcc attttgattc aactggtttt tggaatcctt tgaccccaac
    15001 aacaagttta ggcacacgtt tagttgtttc tacgatttgt gttgttccag ctgtacttat
    15061 gggattgaaa atggacatgt ggtttcgtcc gctagcagat gggcaaaaag gagataaccg
    15121 attaaaaacg ttaaaggaaa tccaacaaat ttatccgcaa ataccagaat caaagggaag
    15181 ttttcctggt tatggcggaa taccaattac tcattataaa aaatactggt atattcagac
    15241 ggatacagta aatacttgta ttgttggtac ttcccgctca gggaaaggcc agatagaagt
    15301 tttagcaacc attgacaatt tgtcgcgtgc agaaagacaa agttcaatgg ttgttaatga
    15361 tccaaaaaca gaattatatg ttgctagtaa agatgaacta gaagctagag ggtatgatgt
    15421 gtatgccttt aatatcaccg atccactgca atcaatgagt gataatccat tggcactcat
    15481 tgtgaaatac tggaagcgag gagacataga caccgcaaca cagttaacca atacttttac
    15541 gaatacgatt tattatgatg caaatgcttc agacaataaa tactttaacg aaaatgcgca
    15601 gaaagctgtc aatgcgctta tttttgcgtt attggaacaa gcggatcaaa caggggatta
    15661 ctccaaatta acaccaaata atctcgttga actactttca gaaattggcg gttttaatta
    15721 ttatccagat cctgagaatg aattcaagca gctcaatgcc ttagatgagt tcatgaatca
    15781 gttaccccct ggaaatatag caaaaaaaca atatggggca acaaaaattg gatctgataa
    15841 agcgaaaggg aatatcttgt ctacggcaat tacaggattg aatccattta ccctaacgaa
    15901 aattgcgaag atgacttctc aatcgaccat tgattacaag tccattggtt ttccgaagta
    15961 tttggatctc aaattacatg aaagcctatt aaatcaacgc attgtcgtgg aattttatca
    16021 taagggaaag aaatttcacg aggaacgagt caaagttggc tatcgtggtt tttgtgaaat
    16081 caactttgat tgtcatttag agcagggaga tcatgtttgc ttaaaataca atcatcaagg
    16141 aaaacaatat gttgcttggt atgagttttc tcaaaaagta ttgaaagacg aagagggaaa
    16201 aatcgtttat cgaaaaaaac gaggagaaac tcatttgcca gaactagaaa aagaagccgt
    16261 tctaacatta gatgagaaga aaagtaacct attagtagag tctattcgga tgcactatac
    16321 cgataaacca atggctattt ttatgttaac gcctgattat gatccatcta accatatcat
    16381 tgttagcatc tttcttagtc agttgtataa agaattatct acgcaatgtg ttaagactaa
    16441 aggagataaa tgtcaccgta ggattcattt tattttagat gaatttggaa atatgccacc
    16501 gatggacaac atggaaggaa tcatgacggt tacggcaggt cgtaatatgt tatgggatct
    16561 gtttattcaa tcttatcagc aactttatgc gaagtatgga gaacaaaacg cagcgattat
    16621 caaagaaaat tgtcagacac atatttatat tatgtcgaca gatgataata ccatcgaaga
    16681 attttctaaa aaagtgggga ataagacggt tgaacaagag aacagtacca tcaattcatt
    16741 aggtatgaat actcatatca atcgcaatgt tgattcagat cgtatcttaa ctcctgaaag
    16801 aatttctact tttttagaag gtgaaacgat tgtactcgct cctttaaaac gacaagattt
    16861 gaaaggtcat aaaatacggc cgtatcctat ttttaatcat ggggaaacga atcttcctta
    16921 tgcttatcaa tatattgaag atttcaatcc atcgtgggat atcaatgata tagacataca
    16981 ttgtacacat gcggacttgg atcttaaggc tttacaaatt gattatgatt atttcctacc
    17041 agatgaatat gctagaaatc tctatcatgg actgcatgat caattagaag aaaaaaacga
    17101 agaaaatatg ttttataaaa tcgttcaatc ttttgagaat gagacggcta aaaaattgtt
    17161 aattcaattt tacgaagaac aagcaacgga tcgtcttcaa gaatatgttg aaaagttgat
    17221 caaaaatgaa gaaatttctg aaatgattag gaaacggctg ttattggaag ctgaaaaggc
    17281 ggtgaaagaa tgatcattgg aggaattttc tctggcatag ccgactggtg gtcaggttta
    17341 ttcggagaag gggatgcaga tcccaaaacc gtaacgaaag tcttacaaga ttatggtaat
    17401 tatctgcagt atcaagatgt attcagttgg atttgtaata cttttttatg gggattgatc
    17461 aaagggttat ataatctcaa tactttatta gaaaaaacaa tttatcaaag ctttgattta
    17521 aaagacttat tgaacgccgc tggtgttaat gaactatttc aaagtttgat tagtaaggta
    17581 ttggctcttt tttgtgtcgt cacattaatt tatttaggct tgaaattttt tatgagtaaa
    17641 cacccaccga aagtcaaaaa tatcttggtt aatttattta tggccatgat cttgattttt
    17701 ggtggtagtt ctttaatcga tgaagggtta aatatcagta aaaactttta tggagacgtc
    17761 actgcagcaa ataaagcaga taaaacgggt tcacctgctt ttcaaattat tcaaaataat
    17821 gtgattgatg tcagtacatt actagcgact gacccgaata aagtagaatc cttaccaaca
    17881 gataaaagaa atgctttaac agcgaaaaat ttcaaaacag cgaatatcaa tgggattatt
    17941 acacctgatc aagctaagaa aatgtctgaa tcaagtgata ttagtacagt acaaaaagat
    18001 cggttaaagg cattacagta tcgactagag ttagacgata atgggaaaga aatccctgtt
    18061 caaattagtg atgaaggttt agcaaaatat gtgtatacgt caggctatcg tcgatacatt
    18121 agtagtccag ggattatttt ggctggggaa tcttctttgt cggtagccta tttatttatt
    18181 ttgtttacaa tcgttacttg cattattgag ctagtattta aaaaattcta tttagtaatt
    18241 gccgcgtcaa cggactttga gacaggacaa cggatgaaaa gtgcgataga agatatttct
    18301 caatccttct tgttattggc gtttacgatt ttagaattac gaatctatac attaatgctt
    18361 tcaggtatcg gtgatttgca tgcggtagga aagatcaatg gattcttgta tgttgttgct
    18421 ttaattgtgc taactattgc gctattcaaa ggctctcaat cggttacgaa gattttcggt
    18481 gttgatacga gtctgaaaaa cggcagcaat tctcttctta gcatgtttgc cttaggaaat
    18541 attgcgaaaa atgtgggtag cggagctaaa aaccttgcaa gtgctggtaa gagtggttta
    18601 agccatttaa atgatattcg taagaatgga tttggccatc gaaaagatat tacccatgat
    18661 aacggggaag aacaatcaga cgataaagct acaactgtac ctaatagtaa gaagacagtt
    18721 tccgaaatgt tcgggaaagc gaaaaatggt gtttctaaag ccgcagaagg tcttggttac
    18781 gtcaatgaac gtgggttaaa aggaacagtt gaagatctta cagaaaaagc caaagacacc
    18841 ttagaagaga aattagacgg cacgagagct aaagaatttg ctgaagctgt gaagaatcca
    18901 tccgaagcac tagaaaaaca aaaagaaaaa gcttcagcag tcaaagaagg actaaaagat
    18961 gcgtatggtg aaggacaagt caatgcagca gtgaaggcaa atacaacaag tgaaaaagaa
    19021 aatcaagaag gtgcagcgat tccttcagcc aaagatcacc aagatgagtt acattctaac
    19081 gaaaattcga tcggagaaaa agattcagaa gaaggacaac atggtcagtt accaaaaacc
    19141 gatccagaac aatatcgtta taaatctaac attaattctg aaaacgaaaa acgcatttca
    19201 gatttaccaa aacgaaatca agaagtacaa acggatgagg attccatcga tctgccaaat
    19261 gataatggca tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttgcc taagacacaa
    19321 aatttaccaa tgaaagatag tcgtttgaat caattcaaag agaatgatca aagctttgtt
    19381 catcaatcac ctgcaggtac tgttcaaatg aaatcaagtc gtttgcctga aaatggaaat
    19441 ctgacaactg tttcttacaa tttagctgaa agtggtgaaa cagagaatgc gtccgttcaa
    19501 aatattgtga caaaagaagc tgcacctaag ccaatcaata aagttcataa tggatacaga
    19561 ccagcaaacg tagcatttac tgcaccgaca aaaataaatg tgacaagaga aactaaaaac
    19621 aggaacaatg aattgaaaga acaactattg aaggatatca aaccataaaa ggcctattta
    19681 agggcctttt tctttataaa acaagtttaa aatggaaagg agaaaattgt ggaggatatt
    19741 ttaatagcta cgtttataac tgcactggga gcatttttaa cgatatttgt ggtgactatt
    19801 aaaggattta aagatacgga tagtttacaa aaagatcatc agtctatttt agatggtcaa
    19861 aaaacacata ccgaaagcat tattgtacac ctttcagaag gattcactta tttgaaagat
    19921 gctatgccca cattcacagt aaaattaacg gaaatatttc attctgttga gcaaatatcg
    19981 tcagaattga aagagcaaag acaacaaaaa gatcgtcaac ttggctcact taattttagg
    20041 caagaaacga tacaagaatc attggagaat gtaaatcgat taaacgatga aatgcaacga
    20101 gttcatttgg aaaatgaaga attacgcgca caaaattatg atttaattca aaagaaaaga
    20161 aatctggaac aaaaaattgt tgaattaaaa gaagaaaatc gtcaattgaa ttatcaatta
    20221 cgacgaggaa aaaatcgttc tcgtgattta ggaaatgaat tagagttgta atatttagaa
    20281 aaacgctgtg agaaatcaca gtgtttttta tttctaaaaa tacaaaatat aaggggtatg
    20341 gttatattat ggataaatac attcatatga aaaatcctcc aagatttagt ggggaatgga
    20401 agtttttcgc ctttttcttt ttagatctat taacgcttgg tggctgcttt ttttgggcga
    20461 aaatagtcaa taatcttgtc cacttaccac tcttttttgc actcttgaat tatggattat
    20521 ggcttgcgat agcgttagtg attgtttggc gaccctataa taaccctggt caacgccaaa
    20581 tttttgcgat tatcgaatct attttcttta cggataagaa ccactatcat gcgttagatc
    20641 ctaaccgaaa ttatcatggt acgtataagg aggatgaata aatgcgagtc aaaggacaaa
    20701 attcagttga taagaaatcc attttgcagt ttggaaaacc agaagagcgc tctatcttgg
    20761 acttatttcc atataaatcc tgtacagaag agggagtctt aattactaga gaagatcatt
    20821 ttcagcgtta ttatcgtgtc gtttccactg acgtggaggg attaaatgaa caagagaaac
    20881 tagaacgaat gaatcagttg accaccgtca tgcgtacgta tgtgccaaca attaagttag
    20941 ttagtttgac cacagagacg gatctaacgg aacaaatcgt gcaaaagcgg cagttattag
    21001 ataaaaatcg tatggaacag tcactaggaa gaaatctaca gcgattaaga aaatatgaaa
    21061 agaaattagt agaggagatc caggaattaa aacgggcaga acaagaacgt cctgacttaa
    21121 gcttcttttt cgttattgaa ggaaagacaa tcaaggattt acaaactaga aatcgtcagt
    21181 taatgcgatc tagtggcatt ttagggttaa aaccactcta caaaaaagaa ttgattaaga
    21241 ttctttatcg aatgaacaac atgaatgacg aataaaaaag atggaggaaa tgattcatgc
    21301 tagatgtgat aaaaacagct ttttcaacgc aaagtaatca aaaggtgatt gatccattaa
    21361 aacaacttgg taatcgtgaa atagagattt taaaatcaaa aggttatgat ttagatttct
    21421 taaaacaagt gatgccacaa ggtgggttga attttgatga agaatatgtg acctatggaa
    21481 atggttattc acgatgtgtg acagtttatc attacacaaa aaatcctact cttttttggt
    21541 tggccaatct catgaacaat cctcatacag tggccacact agatgttagc acagatgaaa
    21601 agcaaaaagt gattgatcaa atcaaccgcg cattagatga attaaatgat cgatcagata
    21661 atgaacgaaa agcgactgac cgaaataaaa gtgcggaaga atacctggaa ctagcggaat
    21721 atgcccgaaa attgacttct aatggggaaa tttctaaaca agtcaaagtg agagtacatg
    21781 tttacggaaa tacgatccaa gaagtggatg aacgggcgca ggaactgatc gaaaatctaa
    21841 aagggatgga ttatcgtgcg gtgacttatc tgcataaaac caaacgagat tggcaatcga
    21901 tgtttaccat gttcgatgaa caagctgaat ggtttggaaa tatcaaaggg caatcacttc
    21961 catcgttaaa cattggcgga ggattcccgt tccaccacca aacgctaaaa gatccaggag
    22021 gcacacaata tggggaaacc atgacaggcg gcccatttgt ctggaatgcc acgtataaaa
    22081 atagtgtacg tttatctacc aataccttgc ttctaggaat gatgggagct ggtaaatcga
    22141 ccgttcttaa aatgatcgcg gaagcccatt tagctgcagg agactttatt tggggctttg
    22201 aaaagggaaa agactttatt cctttcttaa aggaatataa cggtattatg gtgcgtttag
    22261 acggatctga cgggatgatc aatcctttag agatctttgc cacacggacg tatgatgaag
    22321 catccagttt atatgacgat ggttcagtca aagatttgaa gatcaatgaa gcagcgtcct
    22381 atcaaacgca cttagacaaa gtagtctatc aggtacagtt agtgagtcct cagctaaaag
    22441 gaacgatgaa agcagaattc aagacctact tgaatcgttt ttatgaagag tatggaacag
    22501 taccacgcgg ttttacttcc tcaaattcgc gttcaaatac tgatacgcaa gtcacaggga
    22561 aagatccaga agcttatccg acgttcaaag aatttttagc ctttttagga caactggaac
    22621 taccaggggc ttcccaagag aaaaagaatc ggaaagaaga aatggagtct atcgtggaat
    22681 ccttatgtga gacttatggc atgatttttg atggtcatag tacgattcga caccttgacc
    22741 aacaacagtt ggtctttttt gacattgact ctattacagg cctagatcga ggggtacaac
    22801 aatgtcagat gtacatggcc atgacgttga tttggaatca ggcattgatt caaggacgaa
    22861 gacaacgacg cttaattaaa gaaaaacaaa tggaagaaca agacttcaaa cgcttcttgg
    22921 cgatgattga tgagtgccat aatgcgatta atccagattt cattgaaacg gtaaattatg
    22981 tcacaaactt ccaacgagaa atgcgtaaag tagaagcaat gactgtttta gctacacaat
    23041 ccccacaaga aatggtgcca gaaaatatgt cttctgataa cttctcgaaa ctgaagaaag
    23101 tctttgaatt ttcttctacg aagatcttta tgcgtatgga tgaatctatt ttacgtcata
    23161 tagaaggatt agttggtaaa tcaatgacga actcagaatt agaatccatt cctcaacaaa
    23221 atcaaggaga cgctgtgatc aattttggct caaaagaaac cattcgtgtt cattttagtc
    23281 cgaacaaacg tcaattaaaa ttgtttgatg gaggaaaata atggaaaacc aaaatgaaag
    23341 tttaatcaaa cagtatgtta agcgtcgtgc aaagcggcgc ttatttttgt gggtgtttgg
    23401 tacttctgca ggtttgatta ctatattgat cactgtgttt gtgacccttt ttctaatact
    23461 tgcggcaggt tctattgata attcagattc aagtagtggt ggcgaagctt ttacaggaga
    23521 atattcggaa ggcttaccca tttataaaga aatcaaagga agaggccctt tttctgatga
    23581 gattgcgcaa tatgctgtag gtgcagcagt gaagtataaa ttattaccaa gtgtgatcct
    23641 ttcgcaatac ggttatgaat cagcgtttgg tacgtcggcg tccgcaagaa atgatctcaa
    23701 ttattttggg attacgtggt ttgatgggtg tctgtttcct aaaggaactg cgcgtggaat
    23761 tggtggcatt gaaggtggct ggtacatgaa gtttcccaac tcaaaagctg ccttctccta
    23821 ttacggcttt atggtagcca cacaatcaaa ttttaatgct tgtgtcggaa ataaaagccc
    23881 tggcgcaagc ttattgatct taggacgagg agggtatgcc gccgcaggaa ttactgaaga
    23941 cagtgcctat tataaaaatt gtatgtctat tattaataca aataaattga tggagtacga
    24001 tgagtttgcg attaaacact ggagagaagg cggcggaact ggtggtacga ttacaggaag
    24061 ctggggcaat ccattcccag gaagtagctt agataaaaat tcgttcagtg gaggacaact
    24121 ttttgggaaa aatccaggag gagaattccg acctaacggg ttccatgatg gactagactt
    24181 tggttcagtc gatcatccag gaagtgagat tcatgcggtt catggcggaa aagtcgtgta
    24241 cgttggcaat cctggtattt caggtttggg tgcgtgtgtg atcgtgatta attacgatgg
    24301 cttgaatatg gtgtatcaag aatttgcaaa tagtacagga aattctcgag tgaaagtagg
    24361 cgatcaagtg aaagttggtc aagtaattgg gatacgagat acagcacact tgcatttagg
    24421 atttactcga atggattggc gtcaagcaca aggacatgca ttcattgatg atggtacatg
    24481 gatcgatccg ttaccatttt tgaatagcag caaaaaatag atcaggagaa aaaatatgga
    24541 aaataaaac
//
LOCUS       DAFFSV010000031        24474 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFFSV010000031 DAFFSV010000000
VERSION     DAFFSV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.031"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(129..977)
                     /locus_tag="LR110_001142"
     CDS             complement(129..977)
                     /locus_tag="LR110_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL4822114.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1201..3141)
                     /gene="thrS"
                     /locus_tag="LR110_001143"
     CDS             complement(1201..3141)
                     /gene="thrS"
                     /locus_tag="LR110_001143"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBL4822115.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3478..3657)
                     /locus_tag="LR110_001144"
     CDS             complement(3478..3657)
                     /locus_tag="LR110_001144"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBL4822116.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3704..5527)
                     /gene="cadA"
                     /locus_tag="LR110_001145"
     CDS             complement(3704..5527)
                     /gene="cadA"
                     /locus_tag="LR110_001145"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL4822117.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5805..8084
                     /locus_tag="LR110_001146"
     CDS             5805..8084
                     /locus_tag="LR110_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4822118.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8177..8824)
                     /locus_tag="LR110_001147"
     CDS             complement(8177..8824)
                     /locus_tag="LR110_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822119.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9003..9722)
                     /locus_tag="LR110_001148"
     CDS             complement(9003..9722)
                     /locus_tag="LR110_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4822120.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9853..11004)
                     /locus_tag="LR110_001149"
     CDS             complement(9853..11004)
                     /locus_tag="LR110_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBL4822121.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11221..11961)
                     /locus_tag="LR110_001150"
     CDS             complement(11221..11961)
                     /locus_tag="LR110_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL4822122.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11965..12288)
                     /gene="rsfS"
                     /locus_tag="LR110_001151"
     CDS             complement(11965..12288)
                     /gene="rsfS"
                     /locus_tag="LR110_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBL4822123.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12345..12938)
                     /gene="yqeK"
                     /locus_tag="LR110_001152"
     CDS             complement(12345..12938)
                     /gene="yqeK"
                     /locus_tag="LR110_001152"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBL4822124.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12922..13572)
                     /locus_tag="LR110_001153"
     CDS             complement(12922..13572)
                     /locus_tag="LR110_001153"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBL4822125.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13582..13893)
                     /gene="yhbY"
                     /locus_tag="LR110_001154"
     CDS             complement(13582..13893)
                     /gene="yhbY"
                     /locus_tag="LR110_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBL4822126.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13909..15018)
                     /gene="yqeH"
                     /locus_tag="LR110_001155"
     CDS             complement(13909..15018)
                     /gene="yqeH"
                     /locus_tag="LR110_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBL4822127.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15015..15542)
                     /locus_tag="LR110_001156"
     CDS             complement(15015..15542)
                     /locus_tag="LR110_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBL4822128.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15722..16507)
                     /locus_tag="LR110_001157"
     CDS             complement(15722..16507)
                     /locus_tag="LR110_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBL4822129.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16500..17369)
                     /locus_tag="LR110_001158"
     CDS             complement(16500..17369)
                     /locus_tag="LR110_001158"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBL4822130.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17370..18749)
                     /locus_tag="LR110_001159"
     CDS             complement(17370..18749)
                     /locus_tag="LR110_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBL4822131.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18757..19176)
                     /gene="fabZ"
                     /locus_tag="LR110_001160"
     CDS             complement(18757..19176)
                     /gene="fabZ"
                     /locus_tag="LR110_001160"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL4822132.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19201..19677)
                     /gene="accB"
                     /locus_tag="LR110_001161"
     CDS             complement(19201..19677)
                     /gene="accB"
                     /locus_tag="LR110_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBL4822133.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19680..20915)
                     /gene="fabF"
                     /locus_tag="LR110_001162"
     CDS             complement(19680..20915)
                     /gene="fabF"
                     /locus_tag="LR110_001162"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBL4822134.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20934..21671)
                     /gene="fabG"
                     /locus_tag="LR110_001163"
     CDS             complement(20934..21671)
                     /gene="fabG"
                     /locus_tag="LR110_001163"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBL4822135.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21683..22600)
                     /gene="fabD"
                     /locus_tag="LR110_001164"
     CDS             complement(21683..22600)
                     /gene="fabD"
                     /locus_tag="LR110_001164"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL4822136.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22707..22931)
                     /locus_tag="LR110_001165"
     CDS             complement(22707..22931)
                     /locus_tag="LR110_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL4822137.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22984..23946)
                     /locus_tag="LR110_001166"
     CDS             complement(22984..23946)
                     /locus_tag="LR110_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBL4822138.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23956..24396)
                     /locus_tag="LR110_001167"
     CDS             complement(23956..24396)
                     /locus_tag="LR110_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4822139.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6704 a         5438 c         4259 g         8073 t
ORIGIN      
        1 cattaaaaaa taaacttatt tttgaaaatt atgttttctt aagtcgtgaa acactttgat
       61 ggatcattta tttgtggaaa actacgtttt gttattctaa aaaacttata taaaaaatag
      121 taaggttttt atttcgaatc cataaaatat tcttggattg cttctcgtgt ttttttcact
      181 ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc gatcgtagca
      241 aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt tggtctagat
      301 gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg cgtcaacgca
      361 atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc tgcacttcta
      421 cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag ttgcgggagc
      481 aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca tttttgcaga
      541 atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt ctctgccgat
      601 tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac tgtttctttt
      661 atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga ctcatttgct
      721 tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat cgtgattttg
      781 aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt cggcgcactc
      841 aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc aatattaccg
      901 ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat actttcaata
      961 cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt aagtatacca
     1021 tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa tccgcccaac
     1081 agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct ttgaggaata
     1141 cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga aatttaccta
     1201 ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa agatattcaa
     1261 atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg catcttccaa
     1321 ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg cacgtatctt
     1381 atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca tttgcaaacg
     1441 ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga tgattgttgc
     1501 ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta aataagcgac
     1561 aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat gcgtattttc
     1621 cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt ctaattgtat
     1681 agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta gttttggtcc
     1741 gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat ccatagcggc
     1801 tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt tatctgtatt
     1861 gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt cagcatatac
     1921 tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag gacgaacaaa
     1981 tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca aagcacctga
     2041 tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt cacgataaga
     2101 atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc gaagaacaag
     2161 catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat gatcccagtg
     2221 tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat gttggtaacc
     2281 tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg ttgccccttt
     2341 tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca agtcaagttc
     2401 ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga tatattcttt
     2461 caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt gattgtttga
     2521 gtttccacgc caataagcac ctgccactga taataattta aatacttgta tacggccagt
     2581 ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat aagcagtaat
     2641 cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac ttgcaaaaat
     2701 ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt ctttgacgat
     2761 tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt tttgtccatt
     2821 atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt ggatatttgg
     2881 atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga ctaagccaag
     2941 tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga ttggacgagt
     3001 caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta agctttttga
     3061 aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga ctgcaccatc
     3121 tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt gatcagatca
     3181 tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag gacgaaaagt
     3241 tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta acggtccgac
     3301 cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc ctggaaaagt
     3361 ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct ttctcatcaa
     3421 tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa actaaactta
     3481 ttttttccat tccccattta caccgtatgt tccttttttc atctcattga tgatgacatg
     3541 gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat ctttcatcat
     3601 attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga atggcataat
     3661 gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact atgtttgtct
     3721 tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca ctcaaaatag
     3781 agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt agtcccgtac
     3841 ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac tgtttcgcta
     3901 ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga tcatctttca
     3961 agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc attgcaatac
     4021 cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc atcgcaacag
     4081 gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc aagctacggg
     4141 catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca gatccatccc
     4201 cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt gtctttgctg
     4261 cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga tcaattgtta
     4321 catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag gcacgtccaa
     4381 tctttacttc atgattattg actactgctt ggattccttg accggtcact tctttcagtt
     4441 gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga gctaagatat
     4501 ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt tttattccat
     4561 tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct gttttatcaa
     4621 aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt acaacgatcc
     4681 cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct agaatcagtg
     4741 gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt gagaaccacc
     4801 aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa cgatcagcta
     4861 aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata atcatttgat
     4921 actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct ccatttaaag
     4981 accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca cctgtcaaag
     5041 aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt acaagttcgc
     5101 caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata tcctgcaatt
     5161 ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac tgcagttctt
     5221 tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc atgatcaata
     5281 tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg gtaatcgcta
     5341 atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa aacatcaaga
     5401 aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc tggtgaaaaa
     5461 caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat tttttgaaat
     5521 gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata aatttccagc
     5581 tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat aggccttaat
     5641 tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa caaaaatatt
     5701 cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga aaaactatgc
     5761 tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat gagaaacaac
     5821 tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa aatatgctgg
     5881 atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac acagctaatc
     5941 acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa tatcgccaac
     6001 atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa cgcctgcaaa
     6061 cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa gaagaccgtg
     6121 aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa gaaacaatcg
     6181 ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt ggtcccgcat
     6241 tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga caatttaaaa
     6301 tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat gatgactttt
     6361 tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc tctacgatac
     6421 aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg attgaaggaa
     6481 tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg ttgtatcata
     6541 atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat ttgttttctg
     6601 attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca cagacattca
     6661 aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag caacaagaaa
     6721 gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga ttgaccttag
     6781 tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg ttccgtgata
     6841 tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg tataaagaaa
     6901 caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag ctcctgaaaa
     6961 aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat ctggcggaag
     7021 aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa caaaaggagc
     7081 gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt cgaatccatc
     7141 gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac tttctacaaa
     7201 ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg caggaacatt
     7261 tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat gctgtactat
     7321 tctttttact gatgcgccga acatatccag tgacggttac acacaaagct cgctatatct
     7381 ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa gccttgttcc
     7441 caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga aatgaaacga
     7501 tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc caattaacga
     7561 caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt gtgaaagaac
     7621 atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa ggaacaacag
     7681 atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga aaagcccggt
     7741 attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt tatgctcaac
     7801 tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac tttatgaagc
     7861 gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac cgagttttct
     7921 tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc ctttacacca
     7981 tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa agtcctttgt
     8041 taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa agacaactgc
     8101 tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa aaagtaattc
     8161 aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga tattttaatt
     8221 tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct gggtcaaata
     8281 ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga ttccattcaa
     8341 tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc aaaatctctg
     8401 atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg tctatcttct
     8461 catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct aatagtagaa
     8521 agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca gaacgacgaa
     8581 aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg tccaccatac
     8641 actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga tagccttcaa
     8701 cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc acagctaact
     8761 cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg atttttgctt
     8821 ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga tacttatgct
     8881 ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa gcaagacaat
     8941 ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt aaaactggtt
     9001 gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag atcttccagt
     9061 gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac ctcgttttga
     9121 gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt taatgcttct
     9181 tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc ttcttccatt
     9241 acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc agcgtcttct
     9301 cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc ggaaacaccc
     9361 atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg attcacattg
     9421 ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc atagcgcaat
     9481 tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc aataatatgt
     9541 tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg gtttgcatgc
     9601 ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt tgcataaatc
     9661 cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca tttacgtccc
     9721 atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc ctgttccatt
     9781 atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt cttctcattt
     9841 ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg gaatattgcc
     9901 tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg cctctttttt
     9961 tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc ttccagcatc
    10021 ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct cttgcttttt
    10081 gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat atctttttgt
    10141 ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg ttttcatgcg
    10201 cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag tagaagataa
    10261 caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac aagttgaaag
    10321 atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt ctaacgcatg
    10381 gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat gatagcctgc
    10441 acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat tttcctttgc
    10501 ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat cgggatacag
    10561 actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag acagttcttg
    10621 aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac tgctgtaatc
    10681 aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg tttgcaagat
    10741 ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga cgggaagctc
    10801 cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt tatcgatgat
    10861 agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga cctctgctcc
    10921 tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag gattgtactc
    10981 tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat aaaaaagaaa
    11041 tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat aaactattct
    11101 cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc tatatcgttc
    11161 ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca ggcaaaaccg
    11221 ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg cttcgtctgt
    11281 aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc ttaaataact
    11341 ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc gaccagaccc
    11401 ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct cgcttcctgg
    11461 ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat attctggaaa
    11521 aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat tttcttctaa
    11581 cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca tatagcacag
    11641 cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat cgagcatatt
    11701 gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg caatcatcaa
    11761 catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa aggcaaccgc
    11821 taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt gtaaatgacg
    11881 tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat cgtaaatgaa
    11941 agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga ctagcggagc
    12001 atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga aaacatgtac
    12061 gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt caatacgttt
    12121 tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg cgttgatttg
    12181 acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa catttcgaac
    12241 gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg caatttttaa
    12301 ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt ttttgctacc
    12361 cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc aagcaaatgg
    12421 gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca tgcaatggct
    12481 cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc tacataaatg
    12541 attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat tgcatgaaga
    12601 atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac aagaccatgc
    12661 cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac gattaccatt
    12721 ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc aatactagct
    12781 ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc tactccaagc
    12841 acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag gttttctcta
    12901 gaataagcag tatattcatt actatagtcc atattcatac aacccttcat tttgaatata
    12961 atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga tcttttgacg
    13021 tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag gatatggagt
    13081 atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag tcagctcatc
    13141 gattttatac cattttggca aatattctac catatcccca ccgataataa agtaataatc
    13201 tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac ttttcccacc
    13261 acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg ctaattccaa
    13321 catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag gtggctgata
    13381 agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt cagccatcac
    13441 taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt gctttttctt
    13501 ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg tcttagcttg
    13561 cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc tttgatactt
    13621 ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga cgatttcgca
    13681 atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg tattttgaag
    13741 taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa cgatcattgc
    13801 tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg ctttgctacg
    13861 taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt aaatcaatgc
    13921 tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta ctggcgctgt
    13981 tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca cagaaaactc
    14041 aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct gtaataatcc
    14101 acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat gaatatttac
    14161 atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat caaatcttgc
    14221 aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag gtttgatttc
    14281 ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca tttgatggcg
    14341 atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa tctcgatttt
    14401 atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc ccgctgtatt
    14461 tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga cgatatagac
    14521 atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct catgagattt
    14581 cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag cacgttcctt
    14641 catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca ctttgttccc
    14701 aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta gtgaaccatt
    14761 gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta attcattcag
    14821 taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat aatggcgtaa
    14881 tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca aagccgtttg
    14941 cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac agccgataca
    15001 ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg ttttgctaat
    15061 agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca actatctgtt
    15121 gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc acgtatatca
    15181 gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa ttgtttcttt
    15241 gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa aagatcaaat
    15301 ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac aacagggatt
    15361 cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc tggattattc
    15421 catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag attctttaat
    15481 tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta atttgaaaac
    15541 atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc aaccccgtat
    15601 tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca gaaaaagcat
    15661 ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa acaaaaaaca
    15721 actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga tctaatcgaa
    15781 caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact ttatatcgtg
    15841 gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct aactcgcgta
    15901 gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta ccgtctttcc
    15961 acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct tccagcatcc
    16021 agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca ccaatgatga
    16081 tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca atagcctctc
    16141 cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg atcaaggtaa
    16201 tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt cgatagcctt
    16261 caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt cctttttgaa
    16321 taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca atcgcttcgt
    16381 catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc tcttcaaaaa
    16441 aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca tgtgctgtct
    16501 tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg gattttctgt
    16561 ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa gaattcagcc
    16621 ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg tcttccagca
    16681 aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc aaaactggca
    16741 gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc ttcattgcta
    16801 tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat tccttcttgc
    16861 atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt tgttgctcgt
    16921 tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat gataaaatta
    16981 ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc agtgaggact
    17041 gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata ttcgggaaat
    17101 gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt ttcatcaata
    17161 atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg agggcatact
    17221 ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca ctttgcccac
    17281 atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg attgatccga
    17341 atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt ttctggtgtc
    17401 caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc tatgactgca
    17461 ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc tgtgataaat
    17521 tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc atggacgatt
    17581 atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta aactgcactg
    17641 tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt ccctggagaa
    17701 ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc gtgtccttgg
    17761 aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc cacctgtttt
    17821 tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg gattcgagta
    17881 ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat cgttccggca
    17941 ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc ttctcttttt
    18001 gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt gcgttgtaaa
    18061 gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa gatctgaact
    18121 tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt tccaaatgcc
    18181 gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt tgattgaact
    18241 ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg ataaccaatt
    18301 ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact tcctggaatg
    18361 actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc cattgcatcg
    18421 atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat ggaggcaaat
    18481 cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt tgtcacgatt
    18541 gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc cgggccaatg
    18601 cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc tgaatatatc
    18661 gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc gatttcaccg
    18721 cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa aggttaattc
    18781 cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc cggctgaagc
    18841 tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa ctttcttacg
    18901 gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt gttcaagaga
    18961 aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc ctggcataac
    19021 aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat ttttcttagc
    19081 aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca ttggataacg
    19141 atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt cctgctttgc
    19201 ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact cgttttctac
    19261 aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca gtttcatcgc
    19321 ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt tgaaagctgg
    19381 tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa cctcgattcc
    19441 atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg cttgttcagg
    19501 tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca tattcatgcc
    19561 tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca aatcgaattc
    19621 cgttagcgag gattgatcaa attgactgac taattctctg atttcattga tatccaaaat
    19681 tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc taatgaatta
    19741 ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt caagtcaatc
    19801 tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc taatgccagc
    19861 aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt catcgatttc
    19921 gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat tgcttttgct
    19981 tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc gacttgttcc
    20041 ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc agaaccatct
    20101 ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac gatttctcca
    20161 tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag aacgcctgct
    20221 ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc acgattagga
    20281 tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat ctcacaaatc
    20341 gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat gttacggaat
    20401 gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt acaaatacct
    20461 tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc cattggtacg
    20521 aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg gttttcgatt
    20581 gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg attcattttt
    20641 tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc atagatcgaa
    20701 aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg gaaatctttg
    20761 acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat cggtgcgatg
    20821 ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc aataggtgaa
    20881 gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt ttcttatccg
    20941 tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg attttgtgct
    21001 aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat cggaatttgc
    21061 ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt tgtgatgaat
    21121 cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc cacagatttt
    21181 gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat atttccaatc
    21241 aacccagata cactagagag attaatgatg gccccttctc gctgcttcat catttttttc
    21301 aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca ttttttgaaa
    21361 tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt gtttactaac
    21421 acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc tttctcataa
    21481 tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat ttcttctatc
    21541 ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc ttttgcaaaa
    21601 gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac attttttcct
    21661 tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat gtctgcttat
    21721 tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg cttaatactt
    21781 ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta tggatactct
    21841 catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt gtttctttct
    21901 tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag aattgaatct
    21961 gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta tgaaaaggcc
    22021 cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt aaaacagctt
    22081 cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta taatttgcag
    22141 gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt tcaatcggcg
    22201 cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc atataagcgc
    22261 ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta gctactaatg
    22321 ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca tgctccttca
    22381 ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat tgcgtctgat
    22441 ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga atctcacttg
    22501 cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc ataccttgat
    22561 attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc aatatatcca
    22621 gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt caggaaatgt
    22681 cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta agtcactaaa
    22741 tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc atcttcaatt
    22801 tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg gatgtcagta
    22861 gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc ttgaattttt
    22921 tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt ataatttaac
    22981 agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc ctgtaaatat
    23041 tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat ccaataagat
    23101 agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga acttttcttg
    23161 cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct gatgcaaaag
    23221 cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa cgtctcgtac
    23281 ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt ctgaatcttc
    23341 tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac ttctggtacc
    23401 gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta agagaatccc
    23461 acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga ttagcttaga
    23521 tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca ttttttcagc
    23581 catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag cgatagcatg
    23641 agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt aatcgggcga
    23701 cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct gttttatcaa
    23761 tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac atcttcgttc
    23821 acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct tggaaagatc
    23881 attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca ttccaaattg
    23941 ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt tcagtgcatc
    24001 cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga ccatttcacg
    24061 atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc ccaactttac
    24121 aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta ctaacttgtt
    24181 aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt ctgaagttgt
    24241 ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg acagatcttt
    24301 gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga agacactaac
    24361 aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac taattagttt
    24421 gattatcaaa tcttttgatg ttcaaactat atcttaactt ttttcaaaat gcaa
//
LOCUS       DAFFSV010000032        24230 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFFSV010000032 DAFFSV010000000
VERSION     DAFFSV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.032"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            220..1131
                     /locus_tag="LR110_001168"
     CDS             220..1131
                     /locus_tag="LR110_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL4822140.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1509..2426)
                     /locus_tag="LR110_001169"
     CDS             complement(1509..2426)
                     /locus_tag="LR110_001169"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBL4822141.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2628..3065
                     /gene="zapA"
                     /locus_tag="LR110_001170"
     CDS             2628..3065
                     /gene="zapA"
                     /locus_tag="LR110_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBL4822142.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            3123..3671
                     /locus_tag="LR110_001171"
     CDS             3123..3671
                     /locus_tag="LR110_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBL4822143.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            3786..6146
                     /locus_tag="LR110_001172"
     CDS             3786..6146
                     /locus_tag="LR110_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL4822144.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6233..6547
                     /gene="trxA"
                     /locus_tag="LR110_001173"
     CDS             6233..6547
                     /gene="trxA"
                     /locus_tag="LR110_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBL4822145.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6729..8507
                     /gene="uvrC"
                     /locus_tag="LR110_001174"
     CDS             6729..8507
                     /gene="uvrC"
                     /locus_tag="LR110_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBL4822146.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8652..9551
                     /locus_tag="LR110_001175"
     CDS             8652..9551
                     /locus_tag="LR110_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822147.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9544..10806
                     /locus_tag="LR110_001176"
     CDS             9544..10806
                     /locus_tag="LR110_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822148.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            10967..12865
                     /locus_tag="LR110_001177"
     CDS             10967..12865
                     /locus_tag="LR110_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBL4822149.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(13195..13914)
                     /locus_tag="LR110_001178"
     CDS             complement(13195..13914)
                     /locus_tag="LR110_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBL4822150.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            14251..14583
                     /locus_tag="LR110_001179"
     CDS             14251..14583
                     /locus_tag="LR110_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822151.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14570..16573
                     /locus_tag="LR110_001180"
     CDS             14570..16573
                     /locus_tag="LR110_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBL4822152.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16592..17062
                     /locus_tag="LR110_001181"
     CDS             16592..17062
                     /locus_tag="LR110_001181"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBL4822153.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            17083..17667
                     /locus_tag="LR110_001182"
     CDS             17083..17667
                     /locus_tag="LR110_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBL4822154.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17682..18668
                     /locus_tag="LR110_001183"
     CDS             17682..18668
                     /locus_tag="LR110_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBL4822155.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            18658..18969
                     /locus_tag="LR110_001184"
     CDS             18658..18969
                     /locus_tag="LR110_001184"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBL4822156.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            18991..20772
                     /locus_tag="LR110_001185"
     CDS             18991..20772
                     /locus_tag="LR110_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBL4822157.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20765..22141
                     /locus_tag="LR110_001186"
     CDS             20765..22141
                     /locus_tag="LR110_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBL4822158.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            22148..22783
                     /locus_tag="LR110_001187"
     CDS             22148..22783
                     /locus_tag="LR110_001187"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBL4822159.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22813..24168
                     /locus_tag="LR110_001188"
     CDS             22813..24168
                     /locus_tag="LR110_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBL4822160.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT         7984 a         4151 c         5308 g         6787 t
ORIGIN      
        1 agaaattaat tgtttgtaga atgaaggaaa ctatttattg ggtagatgaa tcaccacgaa
       61 tagtcaaaag gagatttcag gatatgccaa aaatgaaaaa aatcatttta gcagtaatgt
      121 tattttctac ggttatatct tttacagccc cttttttgct tacttaagct gattttaagt
      181 tttggccctt agtctggatc tacgaggtga gagtcaagaa tgaaaaaaat aattggagct
      241 tttgtatttg caatcattat cattattatc tgttttcaaa tagatgtttt tggtcaaaaa
      301 aagatagttg gacaaacggt cagcagtacg aacagtacgg ttacaaagac aaaggaatca
      361 tttgatgata cagaacaaga gacattaatc aaaatcggaa aaaatgaccg tacactatat
      421 acagatgact cgctaactac cccacttgca actataaacg gcggagagct ggcggagttt
      481 ctttctgaaa caaaaaatgc ttatcagatc aagacaaatg atggctatac tggatattta
      541 gccctcgaag atggaaccaa agtcacaaag aatatacaaa caaaaccgga agagctatct
      601 gatgcagtga ttgtattaga tcctgggcat ggcggaaatg atactggggc attaagcaat
      661 gatgaacaaa cagaagaaaa agatataaca ttaagtacag ccataaaagt aaaaaaggcg
      721 ctagaggatg ctggagcaac ggtttatttg acccatacta cagatgaatt agtacagctt
      781 ggagatatat gcgactacag tgaagaaaaa aaggcagatg tctttatcag tttacacgca
      841 gactctacgg aatatgcgaa tgaagcaact ggaatcacga catattatta ctatggacaa
      901 gaggaaacat tagcacagac aattgctgac tccttcactg atctgcctct ggattcaaga
      961 ggaatctcca ctgggaacta tcaagttctt cgagaaaatc ttcaaccttc tattttaatc
     1021 gagatgggtt atatgaataa tgatagtgat ttaaaggaat tagtaacaga cagttatcag
     1081 cagcagattg ctgcaagttt gacacaagca ttgaccactt attttcagta ataaataagc
     1141 ccagccaaac aaaaatagct cctcatattt ttcgtttggc cgggcttatt atcgcagatg
     1201 caagctttta aacaccgttt aaggaagagg tcgtgagcct gccgttcaac tctgagtcac
     1261 aaggagcaat ttcaaaaaac agcttctcat gtttgatgac tattgtgact tgtgaccgag
     1321 gagttggcat gagaacaccg tttaagaaaa aggttgtgaa atcaacgttt tgttctgagt
     1381 attagagaaa caaacggaaa aatagttcct catattttgc cgtttgttga gctaataccg
     1441 aggatcagat gattgaacac cgtttattcg gaacaaggga ctgtgacatg acttttgtca
     1501 cagttccatt atttttttag tagcttcaaa gctttttctg tattggcaaa atgaagtttg
     1561 gcatattttt caagcatagg catgccgcct ttctccaata gttttgccgc tacttggtca
     1621 gatttcgttc cagctcctga cggaagggtg attccagctt cagctgatgc tttgtctaac
     1681 tcttccaaaa agctggcacg actgatgata gaagcagcag caacagctaa atgatattgt
     1741 tctcctttag taacgaagta cagtttttct tgtaattgat ttttttctaa acgcgcatat
     1801 tttttaaaat ttgcttccgg cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc
     1861 tcgatttttt ttagcagcaa atggatcgct tgattgtgca aagcgacttt catccgaacg
     1921 gcattgtatt ttggttggat ctcattgtat ttttctggag ataagacaag cagtttatag
     1981 gggatggttt tttttaagat gtcagcaagc tggatgattt ggctatcttt taactctttt
     2041 gaatctctaa caccaagttt acgtaattct gcaagctgat cacgtttcac ataagcagca
     2101 catactgtta gcggaccgaa atagctgcca tttccgacct catcactacc taaaacggat
     2161 aattgactga tattttttgg caaagaagag gctgtttttg ctggcttggc ttgtttatcc
     2221 tgtgcctccc cccatttatc agcttctatt gctgcattcg taccttgaaa catcactttt
     2281 ccggatgtat aagcggtaat ggtcgtcccg tctttttttg cagcaaagct agtataagga
     2341 atctgtttat ttaacaaata agagcgatag tatgctttca tttgactaag ttggtcgttt
     2401 gttgctttga taatgacgtt gctcataaaa ttctcctcgt ttttatacat aaaactatac
     2461 cgcaactcac gacttttcgt tttctaagag attgcgagac atagcatttt tttaaagttt
     2521 atcatataat agttaagtat gtgaaaaata tttgtggaga acggtgaaag aaccaacttc
     2581 atggtacact aaataaaact ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa
     2641 aaacacgcta caaagcggtg attgccaacc aaacatatac tattatcgga cgggaaacca
     2701 agcatcatat ggatatcgta acgaagctga tcaatgaaca gttagcagaa ttgaaacaat
     2761 tatcaccaca aatggataat gaacaagcag caatcttgat ggctgtcaat gcgctttcgg
     2821 atcaattgaa gaaacaagaa agaatattgg agctggaaga agaaacagca gaattaaaga
     2881 aaaaaatgat caaatttaca gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga
     2941 acgaagcccg ggaagtattg aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg
     3001 tggaagcaca acagatttta aacgagaaac gaaaaggaca aattaaacaa aaatcttcta
     3061 attaagatcc ttttatcaaa ataagaaaaa tttaaaaaag ttagaagaga aaggaacgta
     3121 gcatgctgag tttgctgatt ttatttttat tgctaattgc gtttttttct ggagcacgtc
     3181 gcgggtttgc tttgcaaatc atattcgcga ttggatatgt attgtcattt attgctgccc
     3241 agcattttta caaaccatta gcaagtcatt tggaattata cattccatac ccagctgtca
     3301 ccccaacatc aaagttagca ttttttgatc aggtatttgc ctttcaccta gatgaagctt
     3361 tttatgcagg aactgctttt ctgattattt tgctaattgg ctggctgctt acccgatttg
     3421 tgggggtttt tgtccatgga ttgacgtatg tgccgatctt gagacaggta gactggattg
     3481 caggaggcat cctgtcacta atcatggctt acgttactat atttttgatt ttgcagttac
     3541 ttgcctttgt tccattagat gtggtccaaa atcaattcgg cgagcatagt ttcgctcgtt
     3601 ttatcgttga gagaacgcca tttcttacca acaagatgta tgagctttgg gtaacgaata
     3661 ttttgaatta aggaaaatag tgctggaaaa tatttcgttt tttgtacaga gtagtttatt
     3721 taagaaggct gcgtagcgta tgtccggcac agctcttttt tcatagtgaa agataaggtg
     3781 ataaaatgaa taaccgaatc cttgaaacat tggaatttga aaaagtcaaa cagatggtca
     3841 gacaatttgt agtgactgca caaggaaaag aagaactggc agaattggta ccagtgagtg
     3901 agaaacaaac gatcacaaac tggcttcaag aaactgagga tggattgaaa gtgcaacgtc
     3961 tgcgaggcgg gatccctata cctaaaattg aaaatatccg tccccatatg aaacggattg
     4021 agatcggagc agacttgaat ggagtggagc ttgcacaagt ttccagagta ttgtctacga
     4081 caagcgaatt gaatcgtttc attgatgatc tttctgacag tgaaatcgaa tttgcacgtt
     4141 tgtatatgtg ggcagatcag ttagtcacga tccctgtttt gagccgccgt ttaaaggaag
     4201 caatcgatga agacggacgc gtgactgatg acgcttctcc tgagttaaag agcataagac
     4261 agaacattcg gcgaagtgaa caagctgttc gtgaacagtt agatgggatc gtccgtggga
     4321 aaaatgcaaa gtatttaagt gatgcgatca tcaccatgcg aaatgaccgc tacgttatcc
     4381 cagtcaaaca agaataccgc ggtgtatttg gcggtgtagt ccacgatcaa agtgcttcag
     4441 gtcagacttt atttattgaa ccaaaacaag tggtggattt gaataatcgt ttgcgtcaat
     4501 accaaatcgc tgaacgaaat gagattcaac gtattttaag tgaactttct gctgaacttg
     4561 ttccccatcg tcaagaaatc atccacaata cttatgtgat tggtaaaatg gatctgatga
     4621 atgcaaaagc acgatttggt aaagaagtaa aagccatcgt gccgggaatc agtgaagaca
     4681 atcatgtcgt cttgaaacaa gcaagacatc cactgatcga tcaggaaaaa gtagtttcta
     4741 atgacatcac aatcggaaaa gactatcaag ccatcgtcat tacaggacct aacacaggtg
     4801 ggaaaacgat cacgttgaag actttaggtc tgttacaact gatggggcaa gcaggattac
     4861 cgattcctgc aggtgaagaa agtcaaatcg gtatttttga ggaagtattt gcggatatag
     4921 gagacgaaca gtcgattgag caaagtctat cgaccttttc ttctcatatg acgaacactg
     4981 tagatatttt atcaaaagtc aatgaaaaaa gtctggtatt atttgacgag ctcggtgcag
     5041 gaacagatcc gcaagaaggt gctgctctag caatcgcaat cttagatgat cttggtaaaa
     5101 agtctgctta tgtgatggca acaacgcatt atcctgaatt gaaagtttat ggatataatc
     5161 gggcaaatac gattaatgcc agcatggagt ttgatgtgga tacattaagt ccaacgtatc
     5221 gtttgctgat tggcgttcct ggacgaagca atgcgtttga aatctctagc cgattaggtc
     5281 ttgatacaga agttattgat gaagcgaagc agttgatgaa tgatgagagc caagatttaa
     5341 acgaaatgat cacagattta gagaatcgcc ggaaaatggc agagaccgaa tatctggaaa
     5401 tgcgtcattt tgtttctgag gcacaagaac ttcacgatga tttgaaagaa gcttacagct
     5461 atttctttga agaacgtgaa aaagaaatgg aaaaagcaaa gaaaaaagca aatgaagtcg
     5521 tgtcagaggc ggaagaaaaa gcagaaaaaa tcattgctga tattcgtaaa atgcaacagc
     5581 agattgggca aggaaatgtg aaagagcatc agttgatcga tgcaaagacc caattagcaa
     5641 atctacatca agaagaaact ttgaagaaaa ataaagtatt gaagaaggcg aaagaacaaa
     5701 aaacgttgaa accgggagac gaagtgttgg ttacgactta cggccaaaga gggacgcttt
     5761 tacgtaaaaa cgggaatcag tggcaagtag aaatcggtat attaaaaatg aatgtgtccg
     5821 aagacgagct gacgcctgtt gctcctcaaa aagagccgac acaacgagtt atccatgctg
     5881 tacgatctga atctagtagt cacgtgccga atcaattgga ccttcgagga aaacgatacg
     5941 aagaagcgct aagtgaggta gatcaatatt tggattctgc cattctagct ggttatcctc
     6001 aagtgactat tgtccacgga aaaggaacag gcgcacttcg caagggaatt actgattatc
     6061 ttaaaaacca tcggagcgtc aaaagctttg agtttgcacc ggcaaaccaa ggcggaaatg
     6121 gtgcgacaat tgttaaattt aagtaaagct tactaaaggt aagcagatgg ttcgctatcc
     6181 aagtatagaa atgctatact agtcatagat tttaaaaatg gaggtctttg atatgtcaca
     6241 agtaattaca gataagacat ttaatgaaga aacagataaa ggacttgtat tgattgattt
     6301 ttgggcaact tggtgtggtc catgtcgtat gcaagcaccg attttggatc agttggaaca
     6361 agaatatgat gaagaagaat tcagaattgc taaaatggat gtggatgaaa atccagaaac
     6421 tcctcaacaa ttcggtatca tgagcattcc aacattaatg ttgaaaaaag atggtcaagt
     6481 agtagaaaaa gctgttggtg ttcattcaaa agaacaacta cgtcaaatga tcgatcaata
     6541 tttataaaaa cttacagcaa aaataagctg agggtgggac tgaatccagt tccgtcctcg
     6601 gtttttttgc ggttgagtta aggttattca aattaatatt ttttgagtat aattacgttg
     6661 tcaggctgga ttgttttttt gacagacatg ctataataac tttaattgaa tatgaagggg
     6721 agtaccagat gaacgaacga atcaaaaaca aattagcttt acttcctgat cagcccggct
     6781 gttacttgat gaaagataaa aatggaacga tcatttatgt cggcaaagcc aaaatattaa
     6841 aaaatcgtgt ccgctcatat tttactggta gccataatac gaaaacggaa cggcttgtta
     6901 gtgaaatcga agactttgaa tacatcgtta cagaatcgaa tatcgaagca ttgctcttag
     6961 agatcaatct gataaagaaa aatgatccta aatacaacat tatgttaaag gatgataaaa
     7021 cctatccttt ccttaagata acgaatgaaa aatatccccg tttggtgatt actcgtaagg
     7081 tactaaaaga caaagcactg tattttggac catatcccga tgtcggtgcg gcgaatgaga
     7141 caaagaagat cctcgatcga ttatttcctc tgagaaaatg caaacctagt cagacgaagg
     7201 agccttgtct ttactatcat ttgggacaat gcctgtgtcc ttattatttc gacgtagatc
     7261 cgaaagtcta tacgaaaatc gttgatgaag tgaaaaactt tttaaacgga ggacacgaaa
     7321 aaatcgagtc agagatccat cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa
     7381 aagctgcaga ataccgagac cagatccgtg ccattgagac aatcatgact cgccaaaaaa
     7441 tgacaaatac tgatttactg gatcgagatg tatttggtta tgcgattgat aaaggatgga
     7501 tgtgtgtcca agtgtttttc gtaagacaag gaaagctaat tgaacgagat gtttcaatgt
     7561 ttcctttcta taacgaagca gaagaagatt tcttgactta cctcggtcag ttttatcagg
     7621 aaaatgagca tttcattcct aaagaagtct tgattcctga tacgatcgac aagcccagtg
     7681 tagaagcttt gttagctacg aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag
     7741 ttgaattggc gagtaagaat gcctcagttg ctttgaatga acggtttgat ctgattgcca
     7801 gaaaacagga acggacgatc ggagcagttg aacgtttagg aaacgcgatg aatatcccga
     7861 ccccaatacg tatcgagtct tttgataact ctaatatcat ggggacgaat cctgtggctg
     7921 cgatggtcgt attcattgat ggaaaaccgg caagaaaaga atatcggaaa tataaaataa
     7981 aaacagtcaa cggaccggat gattatgcat caatgagaga agtcatctat cgcagatatt
     8041 cgcgagtgat tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat ggaggaaaag
     8101 gacaagtaga tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat attcctattg
     8161 ctggcatggt aaaaaacgat aaacataaaa caaatgagtt gctattcggt acagatttgg
     8221 ctgtagtacc gcttgagcga aattcaccag agtttttctt actccagcga atccaagatg
     8281 aagtccatcg attcgcaatc actttccatc ggcagctacg cagtaaaaac agttttgctt
     8341 cccgattaga tgaaattgaa gggttaggac caaaacgaaa aaaaatgctg cttaaagagt
     8401 tcaaatcgtt gaaaaatatc acagcagcca cgacagaaga attacaagaa atcggactgc
     8461 cgaaaaatgt cgcacaaaac gtatttgata aattacatca ggaatgagga aagggcactt
     8521 tacaaagaaa gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt ttacgttttg
     8581 ttggtaaata ataaatcgct tacgtctggt gtctaaggat atatgtgatt tcttgaagaa
     8641 ggagtgaaga aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac tacacagctg
     8701 tcgatcatgt ctctttccaa atccccgatg gaaaaatcat ggggctaatt ggccaaaacg
     8761 gggctgggaa gacaacgact tttcgtttga tcttggattt tttgacacaa gaccaaggag
     8821 aagtgttatg gaatggccat catcttgggg agaaagatta tgatgtcatc ggttatcttc
     8881 cggaggaacg gggactttat ccgaaagtaa cgattcaaga ccaactgctt tattttgcag
     8941 aacttagagg gaaatcaaga aaagagattg aaccaaaaat cgacttttgg atggaaaaat
     9001 ttcaggtaaa agggaaaaag acggataaag taaaatcttt gtctaaagga aatcagcaaa
     9061 aagtccagct gatcgccacg ttaatccatg aacctaaatt gatcattttg gatgagccat
     9121 tcagcgggtt agatccggta aatgctgaat tgctaaaaga tggaatattg gaattgaaaa
     9181 aacatggttc atgtgtcatt ttttctagcc acaatatgga taatgtagaa aaaatctgcg
     9241 atcatttgat catgttgcga aacggaaaaa tggttttgaa tggaaaagta catgagattc
     9301 gtgaatcatt tggccgtacg aagttattct tggaatctgg tttaagccaa caggaagtag
     9361 aagaaatcga aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta gagattacct
     9421 tagaagatcc tgaagctggg aaagaaatct ttactcgtgc gactcagttc ggctacattc
     9481 ctatgtttaa ccagcaaccg ccaactttgg aagagatttt caaattgaaa gcaggtgaaa
     9541 tcgatgcgta agttttggat tattaccaaa gatgtctatt taaaaaatgt taagtcgatt
     9601 tctttcttga tcatgatatt agtccctttt atcatgctgg gtatcattta tgtagcagga
     9661 aactttgctc aacaaaatag tgatgtcaat aaaatcggcg tattggctga taatgagcaa
     9721 atcagtcagc aaatgagcca agtcaaaaca gatgattttg agttcaaagc agttgattct
     9781 gaagaaaaag cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt agtagtctca
     9841 actgagaatg ataagataaa tgggatgctt tatagcgaaa attcattagg acaaagtaca
     9901 caactactga tacaacaaca actgactggc ttgcaatcga tgcttcgagc gagtcaatta
     9961 ggcatttcac cagaagaagt agctagcttg agccaacaag caagtttgac tcgacaaaaa
    10021 gtcaattttg attcaaaagg aaaaatgaca gtcggagaag ataactcgac agtccaatac
    10081 gtcgtaagtt atgtagcaac gattgtcttg ttcattatta ttttgacgta tgcgcagatc
    10141 attgcacaag agatcgcttc tgaaaaaggc acgcggatca tggaagtgat cctgtccagt
    10201 acgactgcac aaaagcattt ttacggaaaa ttaacaggtg ttttacttgt tgcattaacg
    10261 caaatggcat tatacggtat tatttttggt ataggataca gtcaattcaa agatatggaa
    10321 atggtcaaaa atatattaga cggtatctca ttggataact tgattggacc attcttgtgg
    10381 tattctcttc tattcatgat ctttggcatt ttgatttttt ctgttttagc agctttatgt
    10441 ggctctttgg tcaacaaagc agaagataca gcaaaagcaa ttttgccagt gacttatcta
    10501 tctctaggcg gatacatgct tggattgata ctaggagctt cagatccaaa caatatcgtt
    10561 attcggatca cttcgtatat tccattctta tcttcatata ttatgccgat tcgcttagca
    10621 aatgagacag tcggtatggg tggagcattg atttccttgt tggtacttgt agccgtaaca
    10681 ttcttattga tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt ttatagcgaa
    10741 ggcggattat ggagttcatt gaaacaatcg atttcaatta tgaggaatga acgaaaaaaa
    10801 gcataaaaca atcctaaaat acagaaggaa gctggacatc tattgttccc agcttctttt
    10861 tgtatagacc tattttattt attttactat attggatata tattcattga aaatagtgta
    10921 taacaacttt ataatattga gtataccaat ttttggaggc gttttaatgg accgttattt
    10981 agatttactt tcagaaaaat tcccctcaac ggaggatgtc atcacggaaa tcatcaatct
    11041 cgaagcaatt ttgcagcttc caaaaggaac agagttattt ttgagtgata tacatggcga
    11101 atttcctgct tttgatcata ttctgcgtat cggttcagga aatcttaaag aaaagatcaa
    11161 agaccttttt tctgaacaaa tgacggaaac ggatatcagc caattcacta tttttacggc
    11221 ttatccagaa tatgcattaa cgactgattg gtacaaagaa caagataaat cgcagctgat
    11281 ccatcatttg atcgatcttt tacggtttac gacagtcaaa tatacccggt caaaagttcg
    11341 aaaaggattg ccgaaagaat atagctatat cattgaggaa ctcttgtata tcgatgaccg
    11401 tatcaatgga aaaaaagact atgtaaaaaa aatcattgaa caacttgttt tgatgaagga
    11461 agaagaaaaa tttttaacga aacttgcaca gacgatccaa aaatctgtca tcgatcattt
    11521 gcatatcgtt ggagatattt ttgaccgagg aactcaagca gccaaagtga tggatcgaat
    11581 catggatttt tcttctgtag atatccaatg gggaaatcat gatattttgt ggattggtgc
    11641 gtattgcggc tctgaggcat gtttactgac acttttacgg attgcagcac gctataatta
    11701 tttatttgaa ttagagaaag aatatgggtt gaatttacgt ccattgtttt cttttgctca
    11761 tcaaacttat cagaagaatc cagtatttgc gcctaaaaac agtaaaaatc tatccgaaag
    11821 agagcaagaa gaattagaaa aaatccatca ggcactttct atccttcaat tcaaatcaga
    11881 acatcagtta ttggatcgtc gacctgaatt taaaatggac gatcgtaaat tgctggaaaa
    11941 aatcgactat gaagcatcaa cgattgattt agaaggtcag ctctatgagt tgaaaggaac
    12001 ttgttttcaa acgatccagc cagatgatcc aaaaaaattc ttggaagagg aacaaaaagt
    12061 gattgatagt ttgatgtatt cattccagca ctctttgaga atgcaaaaac atatgacgtt
    12121 tttgatcgaa aaagggggaa tgtatctcac gaacaatcag catgtgctgt ttcatggctg
    12181 tatacctgta gacgagaagg gacagctctt agcatttgaa ttagaccaat cttatcgcgg
    12241 aaaagaactg ctgggctttt ttgaaaaaca gataactgaa agcgcaaaag atctgactgc
    12301 aaaagaagat ttagcaaccg atttgatttg gtatgcgtgg agtgggccat tttcgccttt
    12361 attcggcaag agtaaaatgg cgacttttga acgttatttt ctcacagagc ctcatacgca
    12421 cgtcgagaaa agcaatcctt attatcactt gcgagatgaa gagtggttca gtgaaaaaat
    12481 tttagcagaa ttcgacacaa aagaagaagg gtcagcaatc attaatgggc acacgccagt
    12541 aaaagtaaaa aaaggggagt caccgatcaa agcaaatgga aaagtatttg tcattgacgg
    12601 aggtttatcc aaagcttatc aaaaaacgac aggaattgcc ggttattcac tattatgcaa
    12661 ttcttatggt ttccaaatcg tgacacatta tccgtttttg cctattgaac aacaatttgc
    12721 aaaaaaatcc gaccaaacaa atgtgaaaaa agtaatcgaa cagcaactac cacgcaaact
    12781 caatcgtgat acaacaaaag gtatggaact gcagcagcag attacccagc tcaaaaggct
    12841 gttggactat cgaaaggacg attgagaaga attccagtga acattgttcc atagactgga
    12901 gactgagaca aaagtcgtgt cgcagtctct ttttccggat aaacggtgtt cttctgccaa
    12961 ctcctcggtc acaagtcaca ctattcaaca aacatgaaaa gctgtttttt gaaattgctc
    13021 cttgtgactc agagttgaac ggcaggctca cgacctcttg gtataaacgg tgttcaaaag
    13081 ccagtttctc ggtattaagc caaatttatg caaaaatgtg aaaagccatt ttcacaaaat
    13141 ttttcttaat actcgaaact aaacagcttt ttcacaaccc cctcagaaag tctattaaaa
    13201 ttcattcaca aagaaatcga ataagcgctg ttcagatgga tgtacacggt gggtaagttc
    13261 cggatgccat tgaacaccga ggattttatg catatcatct ttgctttgaa ccgcttcaac
    13321 gagtccatcg ttagaaaaag cgatggcttt taataaaggt gaaagatctt tgactgcctg
    13381 atgatggtaa gaattcacaa aatatggatc ggggagaagg ttgcttaaca aactgtctga
    13441 aacaatcttg acttcgtgag tcgtaaattg cggggcagtg ggctgttgtt catgttttac
    13501 tgaccacttt ggatataatg aaagatcttg ataaagtgta ccttccaaaa cgacattcaa
    13561 tagttggagt ccccggcaga ctgcaaaaat tggttttctt tgtttcaatg cttccaagat
    13621 cagtgcctgt tcaaattggt ctcgttgaat atttgtttcc gttagctttg gatgaggatc
    13681 ttccatgtaa agttgtggac tgacatcttg accacctgca agaataagtt tgtcgatttg
    13741 tccaatcagt tgaggagctg aatctggaga catgactggc agaataagag gcaatccttg
    13801 agcatctaaa actgcatcca cgaatccttg cggtgtatag ctcacttgat ttccttggaa
    13861 agtatctgtt gctcgaatca gttgatttcc ggcaatgccg atgattggtt tcatttgtat
    13921 tccccctaaa gtagacttat tgtaacaagg atttaaaaaa aattaaacta tgtaagactt
    13981 gcccctatac ttatctgttc ttgctgtgtt actatctgta aagagaaatt tcgtgtgaaa
    14041 ttcatgacga tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa
    14101 aaaggttggg cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt
    14161 gaaggggcct gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa
    14221 gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa
    14281 atccgaaaag cagaagaaga aaatgaaaag cgtgctcaaa cactgcggca gcagctgaag
    14341 atctatgcaa cagaaaaaga gcaggaattg gaaacgatcc aaacaaacta tcgacaacaa
    14401 ctaaaacagt tggtggaaga aaaagaacaa tttgaaatac aagaattaga aaaagaaaaa
    14461 atgaaattga aagcagaagc agacaaggtg caagcaactt tgaaagatca atatcaaaag
    14521 catcagagtc aagcgatcgg tgcgatcatt gaaagggtga aagaaactta tggccgtcac
    14581 taagatggaa aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc
    14641 agtacaagga atccaaaacg ttgaaatccg ggatctgtat caaaatagaa caaacaacca
    14701 gtgggtggag aagtattttc cagataccac gattattgat aaagaagcaa aattaagcgc
    14761 tttaggtaat cggttgaaag acatccaaga agctatccaa ttcatacaac accacggcga
    14821 tagccgtcaa aagaaactcc atttgaaacg tcaagaactt tccttacaag atttggaagc
    14881 caattattcc gaagaaaatt tcataaataa attgaaagaa gtccttgatt taaaagaaaa
    14941 gtgggaacaa ctaactgaag aacgtgatcg tttagaagaa gaggaagaat ggcttcttca
    15001 ttggcaacag ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat
    15061 ggggactgtg agtgcagcaa attttgaaag tttcaaggaa gaactgagta agcttgatga
    15121 cgtttatatg gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt
    15181 aaaaaaatca gttgctgtta tagaagaaat tgctgggaaa tacgggttca ataaagaaac
    15241 gtatccttat gaacaaaatc ctaaagaaaa actgcaagag accaagcagc aattatccat
    15301 tgttcacgag cagcaaaaga agataacgac agcgttaggt cagtgcagtg gctatatcca
    15361 agaatttgaa tgggtagaag aagtcacact tgccgttgca gaacgagaaa aaataaaaga
    15421 acggtttatc catgcgactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa
    15481 acagaatttg ctgcatgtgt taaacgaaac cgtatcagaa gaagatatat acgtcagttt
    15541 cgaaacacca aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc
    15601 gcttgtcgaa ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt
    15661 agatccaaca ccttggatga caccatttta tcttgtcttc ttcggaatga tggtcgcaga
    15721 tgttggttat ggtttgctga tgctcattgg gacaatacta gctcaaaaac tacttgttct
    15781 accacgcggg atgaaacgtt tcgtaaaatt ctttgagatt ctctcgatac catcgatcat
    15841 ctggggattg atctacagtt catttttcgg acagtcatta ccaaaagaac tgtttggcat
    15901 ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc
    15961 cgtgattttc ggtctgatac agattttggt tggattattt gtggcagcaa aggaaaatat
    16021 caggagaaaa gattacttga gtgctgtcag tgatggattt gcctggcaag ggatcctaat
    16081 tgggatcgtt attgcgttgg ttggtgccat gttgataaaa aatacagcat ttgtttacct
    16141 tggcgctggg ctatcgatca ttgctgcctt atgtatcttg atcgttccga ttatccaaac
    16201 gccgtctaaa gtcaaaggtg ctgccaaagg tgcgtataat ctttacggac tgaccggcta
    16261 tatcggtgat ttagtcagtt atacacgttt gatggcattg ggtatttcag gcggaagtat
    16321 tggagcggca ttcaatatgc tagttgcttt catgccgcca gctgctcgat tcagtgtagg
    16381 gatccttttg atcatcgctt tacacgcact gaatatgttt ttgacactgc taagtgccta
    16441 tgtccatggc gcacgattgc agtatgtcga attttttggg aagttttata caggaggcgg
    16501 acgtgccttt gatccgctga aaacggcgga aaaatacgtc aacatcaatc acaagaaaaa
    16561 aaacaataaa taattggagg atttttatat aatgatggat tacttaatta gtcaaaatgg
    16621 tggaatggta tttgcggtat tagcgatggc aacagcgacg attttttcag ggatcggttc
    16681 agctaaaggt gttggaatga caggtgaggc agcagcagca ttgacaacaa gtcaacccga
    16741 aaaattcggg caggctctga ttctgcaatt gcttccaggc acgcaaggtt tgtatggatt
    16801 cgttattgcc tttttgattt ttatcaactt aggaaatgac atgtcagttg tacaaggact
    16861 gaactttcta ggtgcttctc tgccgattgc ttttacaggc ttgttttcag gtattgccca
    16921 aggaaaagtc gcagcggcag gtattcaaat tttagcgaaa aaacctgaac atgctacaaa
    16981 aggaatcatt tttgctgcga tggttgaaac atacgcgatt ttaggtttcg ttatttcctt
    17041 cctattagtg ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa
    17101 atcatctctc agatgaacga agcagcagag caggaaagag cggcgttaga acaagaagag
    17161 cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc aagttgaaac agaacatcaa
    17221 aaacaaaaag aaaaacaaat cgaattactc gaaaaaaaat accgtcagct aaggaatcgc
    17281 cagcaagtag aagtacgaca agaaaatttg aatgcgaagc aggaattttt aagacgattg
    17341 tttgcagatg cggtaactga aatggaaaat tgggatgaat ctgaacaaat ccagtttatc
    17401 aaaaatgcat tgtattcatt acctttgaca ggaaaagtag cattcattgc tggagaaaaa
    17461 tcagcagcct atctttctca gacgttgttg gatgaatgga acaatgaact tccctttatg
    17521 atggttttaa gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga
    17581 gttcagtata attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt
    17641 gagattgcca atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa
    17701 atcccttgat tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga
    17761 ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat
    17821 atatttatga cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc
    17881 tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc
    17941 gttatacttt ccataatctg aaggttttga caaaagctga gatgacaaat caaaatctgg
    18001 accatttgta tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata
    18061 cgcaagtttc aaatgaactt cctgaaagca ttatggacag tatacgagaa gtacgtgatt
    18121 attttgaaga gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga
    18181 cagagcagcg acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg
    18241 cttttattga cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat
    18301 atgggttcat gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga
    18361 actttgtaga taaagatatg gaagattaca cgaattttct attagagaca gattatgctg
    18421 attttctgcg ccctgtcatc cgtgagggaa atattgatct cgtaaagctt gaacagttga
    18481 aagatgatta tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc
    18541 cactactagc ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta
    18601 tcggcaaaag aagccatttc gaatcagaaa caatcagaaa gcgggtgaga caagtctatg
    18661 acgcataaga tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc
    18721 tttgatgttc gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca
    18781 aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg
    18841 atcgaacgtt acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga
    18901 tcattaggga tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat
    18961 atcttatagt atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc
    19021 ggacctcttg taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg
    19081 ggagatctag gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa
    19141 gtatatgaag agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct
    19201 ttatctgttg aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca
    19261 ttagatacct ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc
    19321 gcattggacc atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg
    19381 agtgctggag atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg
    19441 gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat
    19501 gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa
    19561 tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc
    19621 actggacagc gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta
    19681 cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat
    19741 gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta
    19801 aatgaattcc ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta
    19861 ttgattgcta atacatcaaa catgccagta gcagctcgag aagcttctat ctatacggga
    19921 atcacaattg cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc
    19981 acttcacgat gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt
    20041 gatgaaggtt atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga
    20101 agagtcattg cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta
    20161 tccccgtctg gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa
    20221 gtattctggg gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg
    20281 atacaaagct attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa
    20341 gctgactggg catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg
    20401 aatgaaatcg tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta
    20461 gaagtggcaa aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat
    20521 acattcactt caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa
    20581 gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta
    20641 tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta
    20701 gatcaaatta ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg
    20761 agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga
    20821 aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat
    20881 tcgtcgtgga caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg
    20941 aacaagtggt atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact
    21001 aggtgtatcg gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa
    21061 cggaccagag atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt
    21121 agctcgggat tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa
    21181 tacattagtt cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga
    21241 attagctgct cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt
    21301 agtctttgct gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg
    21361 tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat
    21421 cgaacgtatc gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa
    21481 gggaatgcat gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga
    21541 gatttctgca gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac
    21601 gaacttagct actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac
    21661 tcaaattccg attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac
    21721 tggttatatt acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca
    21781 gccaccaatt gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg
    21841 aaagacaaga gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg
    21901 aaaacaggca aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa
    21961 gatctatgcg aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac
    22021 gaaccgttcg attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag
    22081 aaccgaatta aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg
    22141 agttctgatg gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa
    22201 acagttgaca actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat
    22261 gcgtcaattc atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc
    22321 tatgacagaa gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat
    22381 cgaagaatta tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat
    22441 catgagtgta aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc
    22501 tttggaatac gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac
    22561 taccctgctt ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc
    22621 gagtgaaatc gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca
    22681 attagaagaa acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt
    22741 aacaaggttg atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa
    22801 aagaggaaaa cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag
    22861 cttctcgcat taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca
    22921 ttttttagta atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct
    22981 gctgtctgtg taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt
    23041 caatttttga tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt
    23101 ctatgctttg cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa
    23161 gcgctgaatc tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg
    23221 gcctttgtga tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt
    23281 ggctggttga aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca
    23341 gggtttgatt tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg
    23401 gtggtatctg ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg
    23461 gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt
    23521 acccttttat taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta
    23581 gtggaaggga atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga
    23641 acggctggat attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg
    23701 atgtttttga tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact
    23761 acgttaggtg tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa
    23821 gcatttggcc gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt
    23881 acgttgacgt tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa
    23941 acttctggca tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt
    24001 acgatgggat tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg
    24061 tacattggac gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga
    24121 acacaatcaa aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg
    24181 acagaaacct agtgatttag taaaataaga aaataaaata atttttcttg
//
LOCUS       DAFFSV010000033        23925 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFFSV010000033 DAFFSV010000000
VERSION     DAFFSV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.033"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            85..837
                     /locus_tag="LR110_001189"
     CDS             85..837
                     /locus_tag="LR110_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL4822161.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            834..1748
                     /gene="pfkB"
                     /locus_tag="LR110_001190"
     CDS             834..1748
                     /gene="pfkB"
                     /locus_tag="LR110_001190"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBL4822162.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1766..3697
                     /locus_tag="LR110_001191"
     CDS             1766..3697
                     /locus_tag="LR110_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4822163.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(3808..5367)
                     /locus_tag="LR110_001192"
     CDS             complement(3808..5367)
                     /locus_tag="LR110_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBL4822164.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5522..7756
                     /gene="pcrA"
                     /locus_tag="LR110_001193"
     CDS             5522..7756
                     /gene="pcrA"
                     /locus_tag="LR110_001193"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBL4822165.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7773..9809
                     /gene="ligA"
                     /locus_tag="LR110_001194"
     CDS             7773..9809
                     /gene="ligA"
                     /locus_tag="LR110_001194"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBL4822166.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9915..10220
                     /gene="gatC"
                     /locus_tag="LR110_001195"
     CDS             9915..10220
                     /gene="gatC"
                     /locus_tag="LR110_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBL4822167.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10220..11692
                     /gene="gatA"
                     /locus_tag="LR110_001196"
     CDS             10220..11692
                     /gene="gatA"
                     /locus_tag="LR110_001196"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBL4822168.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11689..13119
                     /gene="gatB"
                     /locus_tag="LR110_001197"
     CDS             11689..13119
                     /gene="gatB"
                     /locus_tag="LR110_001197"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBL4822169.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13129..14184
                     /locus_tag="LR110_001198"
     CDS             13129..14184
                     /locus_tag="LR110_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL4822170.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14295..15668
                     /gene="rlmD"
                     /locus_tag="LR110_001199"
     CDS             14295..15668
                     /gene="rlmD"
                     /locus_tag="LR110_001199"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL4822171.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15671..15799
                     /locus_tag="LR110_001200"
     CDS             15671..15799
                     /locus_tag="LR110_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL4822172.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            16239..16427
                     /locus_tag="LR110_001201"
     CDS             16239..16427
                     /locus_tag="LR110_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBL4822173.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            16505..17518
                     /locus_tag="LR110_001202"
     CDS             16505..17518
                     /locus_tag="LR110_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL4822174.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(17585..18619)
                     /locus_tag="LR110_001203"
     CDS             complement(17585..18619)
                     /locus_tag="LR110_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4822175.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            18734..20959
                     /locus_tag="LR110_001204"
     CDS             18734..20959
                     /locus_tag="LR110_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBL4822176.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            20977..22860
                     /locus_tag="LR110_001205"
     CDS             20977..22860
                     /locus_tag="LR110_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBL4822177.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            22841..23815
                     /locus_tag="LR110_001206"
     CDS             22841..23815
                     /locus_tag="LR110_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBL4822178.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         7997 a         4374 c         5188 g         6366 t
ORIGIN      
        1 ataattttta tgaacgtttt tgactgattt atattgctag tagaaaaaaa atatgctatt
       61 atttgtattg aggtgatcgt gaaaatgctt acagaggaac gcagacaaaa aatcctagac
      121 ttgcttgaac agcagaaaat cgtgaaatca caagatcttg taaacctttt gaatgcttct
      181 gaatcaacga taagacgtga tttacaagag ctggagaata gtgggtttct agaaagagtt
      241 catggcggag ctaagaaaga gcaattattg aattttgagc agaatatgag tgaaaaatca
      301 ctcaaaaacg ttcacgaaaa acagcaaatc gccaaatcgg cagctgaaac cattaatgac
      361 gaagatgtca tttatttaga cgctggctct actacgttag aaatgattcc ttttctgcaa
      421 ggaaaacaga tcacagtagt gacaaattcc gttcaacatg cggcaaattt agttgatttg
      481 gagcttgcta ccattatttt aggtggaacg ataaagctgg caactaatgc tgttttagga
      541 tctaatgcga tagaacaact aaaaaatttt cattttaata aagcatttat gggaatgaac
      601 ggtgcccatc ttgaacaagg attcacgact ccggatccag aagaggcagc tgttaaacgt
      661 ttagccatga aaaattccga agagacattt gtattgattg atcataccaa attcaaccag
      721 ttgtccttca cctctgttgc acctttggaa gcagccacga tcataaccga gcgttgtcca
      781 ttagaattcg ttaatgaatt ccgtgaaaag acaacaatta aggaggcaat acaatgattt
      841 acacagtaac acttaatcca tcaattgatt atatcgtacg cgtagatggg ttaaaactag
      901 gcgatttgaa ccgaatgaag gaagacttca agttacctgg aggaaaaggg atcaatgttt
      961 cccgtatttt gaaacggatc gattctcaat ctacagcact agggttttta ggagggttca
     1021 ctggcgaatt tatttcgaag tggcttgaaa aagaacaaat ccagtctttg ttcacaaccg
     1081 taaaagaaga tacaagaatc aatatcaaac taaaatcaga cactgaaact gagatcaatg
     1141 gcttaggccc atctatttca gatgaagaaa tcgatgagtt gaagcaagca ttaggaacag
     1201 taggacaagg agatatcgtc gtattgtcag gaagcgcgcc agctagcctg cgaaaaggat
     1261 tttatcaaga actgattgat atcattcgag aaaaaggtgc agatttcgtg attgatacaa
     1321 caggtgatga cctaaaaaat gcacttgtta agaaaccgtt gttgatcaaa ccaaacaatc
     1381 acgaattagc tgagctttac aacacaacct tccaatctgt agaagaaatc attccgtatg
     1441 ggaaaaaatt actagctgaa ggggcacaga atgtgctgat ctcaatggct ggagacggtg
     1501 ctttgctctt cacggaacaa ggaacttata aaacgaatgt attagttcgc ccattgaaaa
     1561 attcagtagg tgcaggggat tcaatgattg ctggctttat tggggcttac agccgtacga
     1621 aagatccaat cgaagccttt aaatggggag ttgcttgcgg aagcgcaaca gctttttctg
     1681 atgacttggc agctcgcgaa ttgattgatg agttgctgcc agaagttgaa atcacaaaaa
     1741 ttgattagtt tcaaggagga taatcatgaa tatcaaggat ttaatggtca aagaagcgat
     1801 gatcatggat ttgcaagcga ctgataaaaa aggcgcaatc gatgaaatgg tccaaaaaat
     1861 gtttgatgcc ggccggattt ccgatatcga aacatataaa gaaggaattc tagcccgcga
     1921 agcacaaact tctacaggtc ttggtgatgg aatcgccatg cctcacgcga aaaacagtgc
     1981 agttaaagaa gcaacagttt tattcgcaaa aagccaaaaa ggagtagatt atgaagcctt
     2041 agacggacag ccaacttatc tgtttttcat gatcgcagct ccagaaggcg caaacgacac
     2101 acatttgcaa gcattagcag cactatctcg attgctgatt gatgcagatt tcgtaggaaa
     2161 attaaaagaa gcaaatacac cagaagaagt ccaagacttg ttccaaacag ctgaagagca
     2221 aaaagaagca gaagaaaaag cagaacaaga aacacaagct gcagaagctt ctgctcatcc
     2281 agaaagaaaa ttcgttgttg ccgttacagc ttgtccaaca gggatcgctc acacttacat
     2341 ggcagaagat gctttgaaga aaaaagcgaa agaaatgggt gtggagatca aagtcgaaac
     2401 gaacggttca gaaggaatca aaaaccgatt gactgctgaa gacatcgccc gtgcagaagg
     2461 tgtaatcgtt gcagcagaca aaaaagtaga gatgaatcgc tttgacggaa aagaactgat
     2521 caatcgtcct gtcagtgatg gtatccgtaa accagaagag ttgatcaatc tagcccttag
     2581 tggtactgca ccagtattcc atggtgatgg aaaagaaagc agttcagaag aaggaaatgc
     2641 ggatggtact atcggacaaa gaatttacaa agatttgatg aacggcgtat cacacatgtt
     2701 acctttcgtt attggtggag gaatcgctat tgcgctttct ttcatgatcg accaatttat
     2761 tggcgtgcca catgatcaat tatcaaatct agggaattac aatcaagctg ctagctggtt
     2821 caaccaaatc ggacaagctg catttggttt catgctgccg gtattagcag gatttattgc
     2881 ttcaagtatc ggtgaccgcc ctggtttgat cgttggattt gctgctggtg cattagctaa
     2941 tacaggcggt gcaggattct taggtgcact gattggtgga ttcttagctg gttatgtcat
     3001 cgtattttta cgtaaattat tcaagaactt accaaaatca ttggatggaa ttaaaacgat
     3061 cctcttttat ccagtgtttg gcttactgat tactggtttc ttgatgttgc tggtcaatgt
     3121 ccctatgaaa gcaatcaatg atgcattgaa cggtttcttg acaagtatga gtggttcgaa
     3181 tgctgcttta cttggtgcat tattagctgg aatgatggcg gcagatttag gcggtccaat
     3241 caacaaagcc gcttatgtgt ttggtacagc aactttagct acgactgttg caactggcgg
     3301 aagtgtcgtg atggcttctg ttatggctgg aggaatggta cctccattgg caattttcgt
     3361 tgctacacgt ttgtttaaga ataaattcag caaaacagat caagatgctg gtttgacgaa
     3421 catcgtgatg ggactatcgt ttgtcacgga aggatcgatt ccatttgcag cagctgaccc
     3481 gattcgtgcc attccaagtt ttattattgg ttctgcttta actggcggat tagtcggagc
     3541 tttcggaatc aaattactgg ctcctcacgg cgggattttc gttgtattct tactgagtca
     3601 cccggtcatg tatttggtgt ttattgctat cggagcagtc gtatctggtg tgatttatgg
     3661 tttattgaga aaagctcctg cagtagcaga agcatagaaa taaaaaagaa gaggctgggt
     3721 tttcccagcc tcttcttttt ttatagaatc atatagaatc aagtagtgta gatgaatagt
     3781 tgttgtaaaa tagtttttcc atgttcgtta aaatgattgc ttcaaatttg tgatagaaga
     3841 tttagcctcg atttcctgct tgattcttgg caagtttgct tcatcagtca agataaccaa
     3901 aatatctcct acattcatga tcgtatcacc gtgagtcaag atttcctgat ttcctctgcg
     3961 tatagagatt agcagcatat ttttaggcca attgaagtct cgtaccatta atccgtctaa
     4021 agaactctct gccgttactg gaaaatcaaa agttgtcctt gcacctttta gtgaggcggt
     4081 ctgagtattt ccaagtagac gttccaataa tgattcgtaa attggattac cgccgagaag
     4141 gtcattgacg atataagcaa caagagcagc caatcctaaa ggcatcagat gattaagaga
     4201 gccaaccatt tctgtcacta aaataattgc tgtcagcggt gcttttccaa tcgctgtgaa
     4261 ataaccagcc atggcaataa cgacaaaatc cttgatgtag attggatcca tcccgaaatc
     4321 aaccataatg tttccataaa agctaccgat taaagccccc aatgtcaata tcggaaggaa
     4381 aatcccccct ggaagacccc caccgtaaga aatcatggaa aaaacgaatc gaagaacaaa
     4441 caaaccgata atcataatga ttggcaagga agcatgtcca aaagctaaaa cgatttgatt
     4501 gcctccgcct agagtactag gccataaata accaattggc aaaatcagca agaaaggaac
     4561 gaacccgtga aaataagctg gtaaaggcaa tcggttgtaa aattttggca aagccagcaa
     4621 gacattctga taaataaaac ctaagatgcc tagaagtact cctaagatca ctaaggaacc
     4681 ataatatttc aacggcaaag aaggaaggtc acctagatat aaaacaggtc tcaagccgaa
     4741 aaagtaaaga gagacgaagt tagcagtgat tgctgaagca aatgatgtca gccaaacaag
     4801 tggagaaaag gagtgatgga tttcttctaa aacaaagagt aatcctgcga taggagcatt
     4861 gaatgctgct gcaagtccgc tacttgcacc gctagaaatt aaaatctttt cttctgaaga
     4921 tggactccga aacaattgtc cagctccttg tccaatggat gcgccaagct gtatagaagg
     4981 tccttctcta cctagaaaaa ggcccgcacc aactgaaagt acaccgccaa tgaacttttt
     5041 ccataaaaca gagaaccagt tcattgataa ttccccgcga agctgtccca ctacttgtgg
     5101 aatcccgctt cccttgatat ccggttcatt tttgaccaga taacccacga aaaatgctgc
     5161 aaggatgcag caaatagcta ggccaattaa gtaaatcggc tgttcccgac tcatttcata
     5221 gatacttcga actaaatcgc ttagcttttc gatagataat cgaaacaggc taacgacgat
     5281 tcctgtcagc agtccaacaa atattccttt tagaataaaa tatatctgag tcttatctaa
     5341 tttcttgatt tttccttttt tttccattca aacgcctcgc tttcttttaa tttagcgcga
     5401 aatcaacgaa ttgacaagga aaaatccata aaaagaaaca aatgattttg aactagctag
     5461 tttaatgaaa gccaagaaaa tgctataatt aacaagattg ataaaagaaa ggatgttgtt
     5521 aatgtcatcc aaagctgaat tattaaacgg gatgaacccc cgtcagaaag aagctgttct
     5581 tcacactgac ggaccattac tgctaatggc aggtgcagga agtggaaaga cgagagtgct
     5641 gacacaccgc attgcctatt tgattgagga aaaagaagtc aatccttgga acatcctagc
     5701 gattactttt accaataagg ctgcaaaaga aatgaaagaa cgtgtcaatg cgattctcgc
     5761 atctggaggc gaagatgtct gggtatcgac ttttcactcg atgtgtgtgc gtattttaag
     5821 aagagatgtt gattttatcg ggtataaccg gaactttaca atcatcgatt cttctgaaca
     5881 gctgaccttg atgaaacgga tactaaaaga actgaacatt gatccgaaaa aatatgatcc
     5941 tcgaagtata ttaggaacaa tctctcaagc aaaaaacagc ctgcagaccc ctcaggattt
     6001 tgcaaaaatg caaggcagtt attacgaaga gattgcggca aaatgttatg ctgcttacca
     6061 aaaagagctt caatataatc aatgtatgga ctttgacgac ctgatcatga atacgattcg
     6121 attatttgaa gaacatcctg attcactcac gtattatcaa aacaaattcc attatattca
     6181 tgtagatgag taccaagata caaatcacgc acagtatacg ctagtcaatt tattggcagg
     6241 tagatttagg aacctgtgcg tcgtgggaga tgcagatcaa agtatttacg gctggcgcgg
     6301 tgcagatatg cagaacatct tggattttga aaaagattat ccagatgctg cagtaatttt
     6361 gctagaacaa aactatcgct ccacgaaaaa catccttagc gcagcaaacc aagtcattga
     6421 aaacaatagc aatcgaaaac caaaaaactt gtggacagaa aacaaagaag gaaacaagat
     6481 cacttactac cgtgcagata atgaacgaga cgaaacccgt tttatcgtag accggatgca
     6541 agaagagatt cgaagcaatc atcggaatta cggcgacttt gccatcttgt accggacgaa
     6601 cgcacaatct cgtgtaatgg aagaaactct gttgaaagca aatattcctt ataaaatggt
     6661 tggagggcac aagttctacg atcgtaaaga aatcaaagat attttagcgt atttgaatgt
     6721 gctggtaaat cctcaagatt caatcagttt tgagcgtatc gttaattcgc ccaaacgagg
     6781 catcggacca ggatctatcg aaaaactacg cagttttgct tctcttcatg aatggccact
     6841 gcttgaagca gcgcaaaatg tggatctggc aaatatcggt ggaaaagcag gacagcaatt
     6901 aggtgcattt ggagagatga tccaagaagt cactcagatg atcccatact tgacagtgac
     6961 agaattgacg aaagaggttt tggatcgtag cggatatcta gaagacttaa aaatccaaaa
     7021 tacattagaa gcacaagcac gcatcgaaaa cttggaggaa tttttaacgg tcacacaaga
     7081 attcgacaaa caattcgaac agcaaaatga agaagacgct gatgcaccag aagaaaaact
     7141 aacggtcttc ttaaatgatc tggcacttgt ttccgacatt gacaatttag aagaagatgc
     7201 ttcccaagtg acattgatga ctttgcatgc agccaaagga ttagaatttc cagttgtatt
     7261 cctgattggt ctagaagagg gggtttttcc tttatctcga gcattgatgg aagaaagcga
     7321 actggaggaa gaacgccgtc tcgcatatgt ggggatcacg cgagcagaag aagcgttgta
     7381 tttaacaaat gctttctcca gaacattata tggacggacg caatataacc gaccttctcg
     7441 ttttgtagaa gaaatcgatc aggagttact ggaaatcgaa ggcatgcgtc ctacgcctaa
     7501 aaaaacgcct gtattcgcta aaaaaacagc atatagctat aaacagccag agacagcggt
     7561 tgttccctct aaaagtgcta ctggagggga aaataacagc tggaaacctg gagataaagt
     7621 caaacacaaa aaatggggtc taggaacagt cgtccgagta agcgggacgt cgaaggatct
     7681 agaactagat gtggctttcc caagtcaagg agtaaaacgt cttttggctg cctttgcgcc
     7741 aattgaaaaa gcttagtagt ggagggtaaa tgatggaaca tgaaatgacg attgacgaag
     7801 ctgctaaacg agcagaagaa cttcgtacaa gactgaacca atggtctcgc gaatactacg
     7861 tagaagataa accaacagtg gaagactatg tttatgataa agaatacgca gaattagtag
     7921 caatcgagga acaatatcct gatttgatca cgtctgattc tcccactcaa cgagtaggcg
     7981 gaaaagtatt agaaggtttt gaaaaagtca ctcatgatat tccgctttac agtttgaacg
     8041 acgttttcag caaagaagag ctgatagctt ttgatcaacg tgtacaaaaa gcagtgggac
     8101 gtgtagttga ctattgctgt gaactaaaaa tagatggact ttccgtttct ttgcgttatg
     8161 aagatggaaa tttcgtgaga ggtgcgactc gcggggatgg aactgtagga gaaaacatca
     8221 ctgaaaacct aaaaactgtc cggtcggtcc caatcaaatt aaaagaacca atgaacatcg
     8281 aagtaagagg cgaatgtttc atgccaaaac ggtcatttgt ccaattgaac caagacagag
     8341 aagcagaggg aaaagatatc tttgcgaatc ctcggaacgc cgcagctggt tctttgcgtc
     8401 agttagattc taaaatcaca gcgaaaagaa atctggacac ttttttatac acagtagctg
     8461 atttcggtcc tatgcaagca aaaacccagt atgatgcgct agaagagcta gaaaaaattg
     8521 gattccacac gaatcgggaa aaacgtctat gtcattcgat tgatgaagtg tggtcataca
     8581 ttgaagaata tcatgacaaa cgtgtggatt tgccctatga aatcgatgga atcgtcatca
     8641 aagtgaatga attctcgcta caagatcaac ttggttttac tatcaaagca ccgagatggg
     8701 cagcagctta caagtttccg ccagaagaag ttgaaacgct aatcgaaaac atcgaatgga
     8761 cagtcggtcg aacaggggta gtaacgccaa cagctatcat gacaccagtc cgagtagcag
     8821 gaacgacagt aagccgtgcc agcctgcata acggtgatta tattaagttg aaggatatcc
     8881 gcttgaaaga tacagttctg atttataaag cgggagacat catcccagaa gtttcgcaag
     8941 tcgtgttaga taaacgacct aaagacagcg aagaatacca attgcctaca cattgtccag
     9001 tttgtggaag tgaacttgtc catttggatg aagaagtagc cttacggtgc atcaatccta
     9061 aatgccctgc tcaaatgaaa gaaggtttga accactttgt ttcaagaaat gcgatgaata
     9121 tcgatgggtt ggggccgcgc gtactagaac agatgtatga taaaaaacta gtagcagatg
     9181 ttgctgatct ttacaaattg acagaagaag agttattgac gttggacaaa ataaaagaaa
     9241 aatcagcaaa taatattttg acagcaatcg acaacagcaa agataattcg gtcgagcggt
     9301 tgattttcgg gttaggaatc cgacatgtcg gagcgaaagc agcaaaaatt ttagcagaac
     9361 atttcggtga tctagaaaca ttgagtaaaa gtgactatga gtcgattatt gcgcttgata
     9421 cgatcggtga tatcattgcc gacagtgtag tcacttattt cagtaacgaa gaagtccatg
     9481 aattgatgaa tgaattaaaa caagcaggcg tcaatttcga atacaaaggg cttcgaaatg
     9541 cccaattgca agaagtcgag tctcctttta aagaaaaaac agtagtcctt actggtaaac
     9601 taacacgatt tactcgtgaa gaagcaaaag agacgatcga aaatcttggc gggaaagtga
     9661 ctggtagcgt atcgaaaaaa acggatatcg tggtagcagg agaagatgct ggcagcaagc
     9721 tgaccaaagc acaagaatta ggaatcgaag tttggacaga agatcaaatg gcagatgcat
     9781 tggctaaaag tcgctcagta gaagaatgaa aaatgagttt tttcagacaa atttcaataa
     9841 acaatttcag acacggcact cttgtgttaa aatagagagc aaagttttta actcgtttag
     9901 aaagaagggg tagtatggcg attagtgaag aacaagtgaa acacgttgcc aaattagcaa
     9961 aacttgcgtt cgcagatgaa gaacttgcaa gttttaccga ccaattaggt aaaatcatcg
    10021 atatggttga aatgctggaa gaagtagata cggaaggtgt tccatttacg tcgaatgtgg
    10081 ttgaaacagt aaacgtgatg cgacaagatc gggcgcaagc tggatggaac cgcgatgaat
    10141 tattgaaaaa tgtgccggaa acagaagacg gctttatcaa agtacccgca atcatcgata
    10201 atggggaggc tggcgcataa tgacagaatt acacaatcgt tctctagaag aactgcatca
    10261 tatgcttgta tcgaaagaaa tcactgtaca agatttgaca aaaacaacat ttgaacgaat
    10321 caaagaaaca gagcctgagg tcgaggcatt cattacattg aacgaagaaa aagcattgga
    10381 acaggcaaaa gctttagatg aaaaagggat tgatgaaagc aacgtccttg ctggtattcc
    10441 aattggtatc aaggataata tcgtaacaaa agatatcttg acaaccgctg catctaagat
    10501 cctttacaat tttgaaccaa tttatgatgc aacggtcatg gataaaatct atcaagcaga
    10561 tatgatccct gttggtaagc taaacatgga tgaatttgct atgggtggaa gtacagaaac
    10621 ttcttatttc aagaaaacaa aaaatgcttg ggacccaaca aaagtacctg gtggctcttc
    10681 tggtggttct gcagcagcag ttgcagctgg acaaattcca gtttctttag gtagtgatac
    10741 tgggggaagt atccgccaac cagcagcttt taacggcatc gtagggatga aacctactta
    10801 tggccgtgtc tcacgttttg gtttgattgc ctttgcttca tctcttgacc aaatcggacc
    10861 aatgacccgt acggtgaaag ataatgcatt agcattgaca gctatcagcg gttatgacga
    10921 aaaagacggt acttcttcag gcgtttcagt tcctgatttt gcagagggac tgacaggcga
    10981 catcaaagga atgaagattg ctttacctaa agaatactta ggcgaaggtg tggcccctgg
    11041 cgtaaaagaa gctgtattga aagcagcaga aacattccgt tcattaggtg ctgttgtgga
    11101 agaagtcagc ttgcctcatt caaaatacgg tgtggctgtt tattatatca ttgcctcatc
    11161 agaagcaagt tccaatttgc aacgattcga tggtatccgc tacggttatc gctctgaaaa
    11221 tgtgaaatca ttagatgatg tatatgtcaa ctcacgttca gaaggattcg gtgaagaagt
    11281 aaaacgccgt atcatgttag gtacattctc cttaagcgcg ggttattatg acgcttactt
    11341 caaaaaagca ggacaagtcc gtacattgat caaacgagat tttgaaaaag tctttgcaga
    11401 ttatgacttg atcatcgggc catcttcacc aacagttgca ttcggaatcg gtgagaacat
    11461 taacgatcca atcacaatgt acatgagtga catcttaacg atccctgtca acttggctgg
    11521 acttccaggt atgtctgtac cggcaggttt atcagaagga ttaccagttg gattgcagat
    11581 catcggtaat tattttgatg aaaaaacgat gtatcaagcg gcatatgcgt ttgaacaagc
    11641 aacagaattc cataaacaac aaccagctat tttaggaggg aaagacgaat gaattttgaa
    11701 acagtcattg gactagaagt ccacgtcgag ttaaaaacca actcaaaaat tttctctcca
    11761 gcgccagctc attttggtgc agaaccaaac agcaatacaa acgtgatcga ctggggttat
    11821 ccaggcgtgc tgccagtaat gaataaagct gcgctggaat tcgggatgaa agcagctttg
    11881 gcattaaact gtgaaatctc taaagatact cattttgacc ggaaaaatta cttttaccca
    11941 gataatccaa aagcatatca aatttcccaa tttgatcaac caatcggtca tgacggatgg
    12001 atcgaaatcg aagtagaagg taaaaagaaa aaaatccgga tcgaacgtgt ccatttagaa
    12061 gaagatgctg gaaaaaacat gcatggtatt ggcggttatt cttacgttga tttgaaccga
    12121 caaggcacgc ctttgatcga aatcgtttct gaagctgata tgcgttcacc agaagaagca
    12181 tacgcttatc tagaagcatt gcgatcaatt atccaattta cagaagtcag cgatgtgaaa
    12241 atggaagaag gctctatgcg ctgtgatgcc aatatttctc ttcgccctta tggacaagaa
    12301 gagttcggta cgaaagcaga attgaaaaac ttgaactctt tatctttcgt gaaaaaaggt
    12361 ttagcttttg aagaaaaacg tcaagcaaaa gttcttcttt caggaggaga aatccaacaa
    12421 gaaacacgcc gttttgacga aacaacgaat aaaacagttt tgatgcgtgt caaagaaggg
    12481 tctagtgatt atcgttactt cccagagcct gatatcccga aattcgtgat cgatgacgaa
    12541 tggattcaaa aagtaaaagc tagtttgcct gagatgccag cttctcgtcg cgaacgctat
    12601 atccgtgagt ttggacttcc agagtatgac gcaatggtat tgacacttac aaaagaaatg
    12661 tctgattttt ttgaagctgt tttggaaaat ggtgcagatg ccaagcaagc atctaactgg
    12721 ttgatgggtg aagtttctgc ttacttgaac agtgagaaac tggaattagc tgagacaaaa
    12781 ttgacacctg agaacttagc aggaatgatt cgtttgatcg ccgatggaac gatcagctca
    12841 aaaatcgcca aaaaagtctt ccgtgaattg atcacaaatg gcggagaagc ccaagaagta
    12901 gttgaaaaaa atggttgggt gcaattatct gatccgaaca aactactgcc aatcattaat
    12961 gaaattttgg ataacaacca acaatctgtc gatgatttca aaaatggaaa agatcgtgcg
    13021 gttggcttcc tagtcggtca aatcatgaaa gcaactaaag gacaagcaaa tcctggtgtc
    13081 gtgaataagc tacttaaaga tgaattagcg aaacgataga ggctgaaaat gaaaaaagca
    13141 cgcgtaattt ataaccccac ctcgggaaaa gaattattga aaaaaaattt ggcggatatc
    13201 ctacaggcat tagaagaagc aggatttgaa gccagcgcct atgcaactac accagaacca
    13261 gattcagcca agaatgaagc atgtagagtc gcagaactag gttttgatct agtagtggca
    13321 gctggtggcg atggcacaat caacgaagtg gttaatggaa tcgctccgct tgaacaccgt
    13381 cctaaaatgg ccatcatccc agctggtaca acgaacgact atgctcgagc attaaaaatt
    13441 cctagagata atattaaagc agcagcagaa gtgataaaaa aacaccaaac cgtaaaaatg
    13501 gacatcggcc tttctgataa cagttacttc atcaatatcg ctgcaggtgg ctatttgaca
    13561 gaattgactt atgaagttcc atcagaacta aagagtattt ttggttatct tgcttatctt
    13621 gcgaaaggag cagagatgct ccctcgagta aagccaatca aaatgcgctt gaagtatgat
    13681 gaaggggaat atatcggaaa tgcttcgatg tttttcctag ggctgacgaa ctctgttgga
    13741 ggattcgaac agatcgcgcc agatgccaaa ttggatgatg ggaagttttc tttaatcatt
    13801 gtgaagacag caaatatctt tgagatcttg catattgcag cactcatgct gaatggcggt
    13861 cgacatgtcg atgatccaag aataatctat accaaaacaa gcagtttgtt cgttgaaaca
    13921 gaagatgata tcaatcgacc agtaatgatc aacttagatg gtgaatacgg cggggaagca
    13981 ccgatgcatt tccaaaatct tcatcaacac attgagttct ttgccaatac cgatcagatt
    14041 cctgatgaag caatcacagg tacagatgaa gaagaattag aggttgtcag caaagaattc
    14101 gtaaaagaag tcgaacgttt aacggatgaa gatatcgacg gtgacggaaa aatagcagac
    14161 gaaacaccta atcaaaagaa ttaatcaaaa gccggtgctg aacgtagtaa ggtcagtgcc
    14221 ggttttagtt gtgtaattag ctgatttata gtagagttca gtaggattga aaaataggct
    14281 taaaggagca aaatatgaca gaacagcaaa tagtaaagaa aaacgaacga tatacagtcg
    14341 aaatcgtcga cttgagttat gaaggaatgg gggtagcaaa aatcgatggt tatccattat
    14401 ttatcgaaaa tgcactccct ggagaagaag tagaaatact agtagtaaaa gcaggcaata
    14461 agtttggata tggaaaagtc gaaacattta ttacacaatc acctgatcgt cagcctgtag
    14521 aaaacaatgc attacttcgc ataggtatcg cacctttagc ccatttgaag tatgaacaac
    14581 agcttgtctt caaacaaaaa caagtagagc atgtaatggc gaagattgct aagatgccag
    14641 aagttcctgt acttccaacg gtcggaatgg cagaaccatt cggctaccgt aataaagcac
    14701 agatccctgt tcgccgtatc gatggacagt tagccaccgg tttttacaaa aagaatagcc
    14761 atgatctagt ccctatcgaa gatttttata tccaagatcc tgtcatcgat caagcaatca
    14821 aagtagtcag agatatcttg caacggttcc aagtacgagg atataatgaa gaaaaaaatg
    14881 aaggacagct tcgtcacatc attatccgtc gtggtcatta ttcccatgaa atgatggtcg
    14941 ttttagttac tcgtaaagaa aaattcttta aatccaagga aattgctgaa gctattcatg
    15001 aagaattgcc agaagtggtt tcggtcatcc aaaatatcaa tgaagaaaaa acaaatgtga
    15061 ttttaggaga caaagaaaaa gttttatatg gcagagacta tatcgaagat cagctattag
    15121 gaaaaaccta tcgaatctca gcaaagtcat tttatcaagt aaatactcaa caagcagagt
    15181 atctgtacca aacagccatc gagtttgccg agttagataa aaaagacatt gtgatcgacg
    15241 cttattcagg aataggtacg attggcttgt ctttagcaga cagagtggat aaggtgtatg
    15301 ggatggaagt tattccagaa gcaattgaag atgcacagtt caatgcttta aataataaaa
    15361 tcgaaaatgt tcattatgaa gtcggaaaag cagaaaaagt tatgaaaaaa tggcaagaaa
    15421 aagggatcaa accatctgtg atcgtagttg accctccacg aaaaggatta gatcatcatt
    15481 ttattgaatc agcaatcgaa atgtcaccag aacgaatcgt ctatatctca tgtaatccgg
    15541 caacttttgc aagagatgct aagttatttg cagaagcagg ttatgccgtg aagaaagttc
    15601 agcctgtaga tcttttcccg caaacccatc atatcgaatt agtcgcttca ttcgttaaag
    15661 gagaataaaa atggaccaaa aagaaaaaat gaaagaattg atcaaaaaga aacaaggtgg
    15721 aggccgatcc aaacaaatgg aaacacctaa gaatgatcgt aaaaacatga gaaaaggccc
    15781 taaaatcttt aataaataga cgtgctaaag aaagactaag aagtgttaac ttttgttatt
    15841 gtttcttagt ctttttagtt agtctacatt attttggcga tttgccaata aggactgttg
    15901 agttgtgcaa aatcaaatct gggtcagaat gaacgtcatt ctgtctcaga tttttcgtta
    15961 tttcaatttg attaactgat tgatcaaaaa gatttttatt ttcatattct caaatgattc
    16021 aacactaaat atacatgttt gacgcggaaa ttattattcg attttggcgg catcaggggg
    16081 gatgacgacg tagcagaccc attattttac tcaagaaaat gtccgtgcct attatttaga
    16141 atacgatagt gaacgtgctg ggagttttgt tcttttagag aaagtctctg aagataaatt
    16201 agttgtctta gattttgact gtaacgaaaa atggcgaaat ggaagagcac cagcaagtga
    16261 ttgaccggat ctacgaagcg acacgatatc tgcctttgga tcgtttgtgc ttaagtcctc
    16321 aatgtggatt tgcttcaaca gaagaagaaa atgttttgtt tgaagaacaa tggaaaaaat
    16381 ttcttttgtc aaaagcattg cagaagaagt ttggggaaaa tcattaaatc atctaggaga
    16441 gcttgatagg aaataacact atggtataat cagaatgctt aatactaact actgagggag
    16501 ccttatgaaa aagaaaattc ttaaatttat cggatggttc ttcggaatca ttcttgggat
    16561 cgcgtttgca atctttcttg ccttccagct ttcccccaga cctggagcat tcttgatcaa
    16621 ccatatgttt tcaaatactg ttcaaatcac tgataaaaaa acgttcgatc aagcagaaaa
    16681 aaacgtggcg aaaaaaacag atttgacgta tgagtccaag tataaagaga atacttatga
    16741 catctactat ccaaaaaaca gcaaggggcc tgttcctgtt ctattttggg tacatggtgg
    16801 aggattcgtc ggcggagata aatcgggtgt caaagaattt gcgacaaaat tagtttcaga
    16861 cgccaacatc gcagtggttg cgatgaatta tgaattagcg cctgattcag aataccctaa
    16921 tcaagttctt caagtgaatg aactgatcaa agatttatta gctgaagaaa aagacgacaa
    16981 attgctagac atggaacaat tattttttgg tggagatagt gcaggttcac aaatcgcatt
    17041 gcaatatgca gctatacaaa cgaatgatgc gtatgctaaa caaatgaaaa tcaaacatct
    17101 acttccaaaa gatagtctga aaggaaccat ttcttattgc ggacctgttg atttgaagct
    17161 aacagcacag cagcattcgg atgatcgatt tatgaagttt ttcgtcaaaa ccgttgcgtg
    17221 gtcgttgatt ggaacaaaag attggaaaac gagtgagcaa ttgaaagaag ctagtgtggc
    17281 tgaccatgtt tctaaggaat ttccgccaac gtatataact gacggaaatg catactcatt
    17341 ccaggagcaa ggtcaagcat tagaaaatcg gttgacagaa ttaaaggtac ccgtgcaatc
    17401 cttgttttat acagatacaa aaaaagagat tacccatgaa taccaatttg attacacgct
    17461 aaaagaatcg caaaattgtt accagcagac attggatttt gtgaataagt ataagtaaga
    17521 ggttgtgaaa aagccgtttc gttttgagta tgaagaaacg aagcggcttt tcacaacctc
    17581 tctttcaata cctttttgtt cttgggataa aactttccct aacaatcaat cgtgcaggat
    17641 aagtcaaaat gattggttcg tcccgtatac cgtcaattcg ctccaatgcc attcgaacag
    17701 ctgcttgccc aattgatttc gcattgattt gaacactggt caagccaggg gagaggtttg
    17761 cgattgtctc gatattatca aagcttgcta caacaatctc ttctccaatt tggatatttt
    17821 tttgtttcaa tgcccgatag acaccaattg ccattggatc gctcccgaca agaatggcag
    17881 taggaagagg ggattcattt tctaatagat tagtcgcaag tacctcacct tgtttttctg
    17941 tccagccacc tagatgataa gacatatatt tttctaagtg atgatcgatc ataaaatgct
    18001 gataggcacg catcctcttt tcatttatag aaatctgctt cccaccgaat tcatccatat
    18061 cgactttata tcccccgata taggctattc ttttatgacc attttctaag aacaattcca
    18121 gtacggtttt agtcatccgt tcaagatctg catacaccat atcgacctct tgctggatgt
    18181 cagggttatc cacgacgact atttgcatgt tttgttttaa caaggcggta atcgattctt
    18241 tcgtaacagt cccaacaacg atcaccgcac ctaactgatc tagatcttcc cattgtttag
    18301 gattatcaga tagattatag attcgattca tccctaaatg cagacgtcga gcttcttctt
    18361 ccatccctac tcgcaaatgt ttgtaataga tatcctgttc ttcgctcgct ttgccgatcg
    18421 tagagatcac accaatcgaa ggcattctct ttttgatata tttacgagta ctgatgtcat
    18481 aatccaacgc agcagctgcc tctactatcc gttgccgagt agtctgagcc acatggatcg
    18541 tctgatcgtt attcaacaca cgggaaactg tagcaggaga gacatttgcc tttcgtgcga
    18601 catctctaat tccaaccatt tttacacact cctcaaaaaa ataaaaaatt tttattgaca
    18661 aaattgttta gtaaacagta tacaggaatt ataaaaaaaa gtaaacggtt tccgttttgt
    18721 tgggagtgaa atgatggata ttacgcatag aaaaacagat aaaaatttca tcttatttga
    18781 tgaaaagagc cgagtcttcc atttacgaaa tacatttctt tcttatctga taaagataga
    18841 agaaagcaat gtattggcgc atatttattt tggcaaacca gtcaagcagt acaaagacaa
    18901 taaaaattat ccccgaagag atcgtggatt ttcaggaaat gttcccttaa atcctgaccg
    18961 gtctctgtcg aaagatacac taccgcaaga atactcttct catggcagca tggactttcg
    19021 aacgcccgca tcgatcattc agcgcaagaa tggatcagac ttactggatc tgagatatga
    19081 cagccattat attacagatg ggaagccaga gatcgaaggc ttgcctcaaa cttatgtgat
    19141 ggacaagtca gaggcacaaa cgttggtgat ttcacttaag gatagagaaa cagcgattta
    19201 tttcgatttg ttctatacca tatttactga tagagcagtg atcacacgat ctgtaaagat
    19261 aagaaatgaa accggagaaa caatcaaact agaaaaagcg gctagtttcc agctagactt
    19321 tgcccatact aggcgatttg atgaagtcat tgctttgcca ggagcacatg tcaatgaaag
    19381 acagatttca cggcaatctg ttttatctgg aacaaaagta tttgaaagcc gtcgaggaac
    19441 gagcagtcac catatgaata actttattgc tttagttcat catcatacga cagaaaatac
    19501 tggagaagcg atcggacttc aatttgttta ttccggcaac cattctttcg aacttgaaaa
    19561 agatcagatc aatcaattga gagtcgtcgg tgggatcaac agccatcgtt tttcgtggga
    19621 attgaatgca ggccagtctt ttcaaacacc tgaaatgatc ctttcttact caagtcaggg
    19681 attgaacaaa atgagtcaga tccaccatga gttgctacgt gaaagaatcg caagaggtcg
    19741 tcatcaattt gctgaacggc cgattctggt caataactgg gaagccactt actttgactt
    19801 caatagtgaa aaaatcaagg cgatcattga tgaagcaaag gagctaggca tcgaaatgtt
    19861 cgtattagat gatggctggt ttggaaaaag agatgcggac aattcttcat taggagactg
    19921 gttcgagtat gaaggcaagt tgacaaatgg attacgtgaa atagcagatt atgctcatag
    19981 caaaggattg aaatttggtt tatggtttga acctgaaatg atctcggttg attctgaact
    20041 gtatcgaaca catcctgatt ttcttatgca ggagccaggt agaatgcctt ctgcttccag
    20101 atcacaacat gtactggatt ttacaaggtt agatgttcgg cagacgattg agaaacaaat
    20161 gagaaagatt ttagatacga ttcctcttga ctacatcaaa tgggatatga accgttcgct
    20221 ttcagatgtc tattcgatta cccttgaccc acagagacaa ggagaagtag cgcatcgtta
    20281 tatgctcggg ctttatgaac tacttgaaca tttgacaact gattatccgg aaatcttatg
    20341 ggaaggctgt tctggtggag gtggacggtt tgatgccggg tttatttatt atatgccgca
    20401 gtcatggaca agtgataaca cggatgcagt ggagcgtatg aaaatccaat atggaacaag
    20461 ccttgcttat ccgatttcgt ccatcacagc tcatgtatca gctgtaccaa atcatcagac
    20521 cgacagatct acttcgttga aaacaagagg agaaacagct atgagcgctg tttttggtta
    20581 tgaactggat ttgacaaagc tgtctcctga agaagaaaag caagtaaaag accaaattat
    20641 atcctatcaa acgattcgtc cggtgatcca gtatggccat tattatcgtt tggcgagtcc
    20701 ttttgaagaa aatattgccg catggatgtt tgtgtctcct aaacaagatg aagctattgt
    20761 gtttttaggg aggatactag cttcagctca accagcgttc catgaagtat atctgatggg
    20821 gttagatgat gaggcacttt atcaggaaca gacctcgaag cggatatttt cgggggccga
    20881 attgatgaca gttggacttt acttccccga ttttcaaggt gatttccaaa cagaactgct
    20941 tcatttcaaa aagttatgag agagaaggaa aaaagtatga aagcaaatat aaaatggtta
    21001 gatgatccac aagtttttag agtgaaccaa tcaaatgcac atagcgatca tgtttgttat
    21061 gcaacaaggg aagaagtaga aaccacatcc tcccttaaac agtctttaaa tggatcatgg
    21121 cgtttttgtt attcgccaaa tccttcggta cgaccaaaaa cattttataa aaaagatttt
    21181 gatgcctgtt cttttgatga aatcactgtt cctagtcata ttgaattagc aaattatgac
    21241 aagatccatt acatcaatac gatgtatccg tgggagggac atgtatttcg ccgtccccct
    21301 ttcacgacaa gccctgagaa agaggcggaa gggatctttt cagctgctaa agacaatccg
    21361 gttggttcgt atttgacgac ttttcgatta gaagaaggtc taaagggaaa acaaatctcg
    21421 attcattttg ctggtgtaga acaagcaatg tttatttggt tgaatggtca ttttatcggg
    21481 tatgccgaag attcgttcac tccgtctgaa tttgatttaa caccttattt ggaagaagaa
    21541 aatattctag ctgtggaggt ttacaaaaga agtacagctg cattcttgga agatcaggac
    21601 tttttccgat tttttgggat cttcagagat gtcttcttag ttgctcgagg acaaaaccat
    21661 ttagaagatc tttggatcaa acctcgctta gatgacaaat gtcaaaatgg caaacttgaa
    21721 gtagatttgt cttttgagca agtagccaaa aacagtcagc tgaatgtagc gatactagat
    21781 cctgatggga aggaaatcta tcaacagcaa tatccgttaa aagagcaaat gacgattcgc
    21841 atcgatggat ttgaaaatgt ggcactgtgg gggcattttc agcccaattt atataagctt
    21901 gaattggaga cgatagacga agaaaaaaga gtaacggagt atattgacta ttccttcggt
    21961 tttcgtaaaa tcgaaatcaa agataaaatc atctacttca atggaaaacg gatgattatc
    22021 aacggggtaa atcgacatga gtggcatccg aaaaaaggac gcgccatcac tatagaagat
    22081 atggaaaaag atttggctat cataaaaaat gcaaagatca acggcgtacg gacatctcat
    22141 taccccaacc agatcacttg gtattattta tgcgaccaag caggaatcca tgtgatggcg
    22201 gaaacgaacc tcgaatccca tggctcatgg caaaaaatgg gcgcatttga gccttcttac
    22261 aacgtaccag gtgcagtacc tcagtggcag gcggctgttc ttgatcgagc aaaaacgaat
    22321 ttcgaaacat taaaaaacca tccaagtatt cttttttggt ctcttggaaa tgaatcttat
    22381 gcggaagaaa acattcgaca aatgaacgat tacttcaaaa aagtagaccc cgatcgactt
    22441 gtccattatg aagggaattt tccaaatcca gcattcgaag attcgatttc agatgtaaaa
    22501 agctgcatgt atgcgccacc agaagagatc attgactacc ttgaaaataa agcggaaaaa
    22561 ccgttcatct tatgtgaata tatgcatgac atggggaatt cgttaggtgg aatggattct
    22621 tacatgacgt tgcttgatcg ttatgaaatg taccaaggag gctatatatg ggattttaaa
    22681 gatcaggcga tccaagtgac tgatccaatc acaggcagag aagtgatgcg ctatggcggt
    22741 gactttgacg ataaaccaag tgactatgaa ttctcaggga atgggatcgt ttttgcagat
    22801 ggacaagaaa aacccgccat gcaggaggta agatattatt atgaaaaata cagtaaataa
    22861 aagtcatatg gatacggaaa aagttgcaat cgtcttcggc gactgtacat taggtgtcaa
    22921 atcggggaat acgcattata ttttttctta tacaagaggc ggactggaat cgctcaataa
    22981 aaacggaaaa gaatggctat accgggaaac acttccagtt ttttggcgag caacgaccga
    23041 taatgacaga ggtagcggat ttggatttaa atccggtatg tggttaggag ccggattgta
    23101 tccaaaagtc aaagaaatag aagtactggt ggacaataaa aagatcgatt tgccgattgc
    23161 tccagtaaat aatcaatatt caagtaatga atacgctgaa acggttgaaa tcatttttac
    23221 actggaaatc aacacagtac caaaaaccca agtgacaatt tcctaccaaa tcgctggaga
    23281 gggtaaaatg cttgtcaata tgcattatta tggacaaaaa ggattaccgg aattacccgc
    23341 attgggttta aggttcatta tgccaacgaa agcaaaattt tttgagtatg aaggcctatc
    23401 aggggaaaca tatccggatc ggatggctgg tggtgtcgaa ggtatttata aagtggcagg
    23461 actccccgtt tccccatacc ttgttcccca agaatgcggc atgcatatgg aaacgaaatg
    23521 ggtagaacta acaagaaatc aaacgttgga taatacggat aaagatcaag aagatttttc
    23581 gttgcgattt gaacagcagg atacgccatt tgctttttcc gctttaccat tcaaagcaga
    23641 agaactggaa caggcaacac atatagaaga actaccgcca gaaagacgaa cggttcttac
    23701 gatttttgca aaagtaagag gagtcggggg tatcaatagc tggggagcgg atgttgagtc
    23761 accttaccga atcagcagcg aagaggacca tgaaatcggc tttattattc agtagcaatc
    23821 tttagattat tatttgaaaa acggagttta caagacaaag ctgctttatt ctatatgtca
    23881 tgaacaagag agtaaaaaag ttctcagata cttttgttca tgact
//
LOCUS       DAFFSV010000034        23657 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFFSV010000034 DAFFSV010000000
VERSION     DAFFSV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.034"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            255..530
                     /locus_tag="LR110_001207"
     CDS             255..530
                     /locus_tag="LR110_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822179.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            543..2621
                     /locus_tag="LR110_001208"
     CDS             543..2621
                     /locus_tag="LR110_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBL4822180.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2781..4667
                     /locus_tag="LR110_001209"
     CDS             2781..4667
                     /locus_tag="LR110_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL4822181.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="LR110_001210"
     CDS             4679..5626
                     /locus_tag="LR110_001210"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBL4822182.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="LR110_001211"
     CDS             5646..6173
                     /locus_tag="LR110_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL4822183.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="LR110_001212"
     CDS             complement(6236..6889)
                     /locus_tag="LR110_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBL4822184.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="LR110_001213"
     CDS             7021..7863
                     /locus_tag="LR110_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL4822185.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="LR110_001214"
     CDS             8021..8917
                     /locus_tag="LR110_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL4822186.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="LR110_001215"
     CDS             8920..9633
                     /locus_tag="LR110_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBL4822187.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="LR110_001216"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="LR110_001216"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL4822188.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="LR110_001217"
     CDS             10168..10395
                     /locus_tag="LR110_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBL4822189.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="LR110_001218"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="LR110_001218"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL4822190.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11681)
                     /locus_tag="LR110_001219"
     CDS             complement(11181..11681)
                     /locus_tag="LR110_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL4822191.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="LR110_001220"
     CDS             complement(11960..12358)
                     /locus_tag="LR110_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL4822192.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="LR110_001221"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="LR110_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBL4822193.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="LR110_001222"
     CDS             13471..14238
                     /locus_tag="LR110_001222"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBL4822194.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="LR110_001223"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="LR110_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBL4822195.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="LR110_001224"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="LR110_001224"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBL4822196.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="LR110_001225"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="LR110_001225"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBL4822197.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18894..19799
                     /gene="xerC"
                     /locus_tag="LR110_001226"
     CDS             18894..19799
                     /gene="xerC"
                     /locus_tag="LR110_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBL4822198.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19836..20384
                     /gene="hslV"
                     /locus_tag="LR110_001227"
     CDS             19836..20384
                     /gene="hslV"
                     /locus_tag="LR110_001227"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBL4822199.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20398..21798
                     /gene="hslU"
                     /locus_tag="LR110_001228"
     CDS             20398..21798
                     /gene="hslU"
                     /locus_tag="LR110_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBL4822200.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21816..22607
                     /gene="codY"
                     /locus_tag="LR110_001229"
     CDS             21816..22607
                     /gene="codY"
                     /locus_tag="LR110_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBL4822201.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22689..23564
                     /locus_tag="LR110_001230"
     CDS             22689..23564
                     /locus_tag="LR110_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBL4822202.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8193 a         4079 c         4982 g         6403 t
ORIGIN      
        1 tttgtcgcag ccccattttt ttgttgtgat aaaagaacaa gaaaggcagg aatgggaaag
       61 taaacatcct tttgtcagaa acttttatct ttcttaagtt ttacttgata tctgatacta
      121 tttggaatag aatgaggcgt tgaaaaattt atggaaattt ctaactagca ataaacgatg
      181 aaacaatgaa atatttttca tccaaaaaga actttgctat aatgatggaa gtttgtgtat
      241 aggagatctg gaaaatgaga gagagttttg aacaacaaaa aaaactgttg catgatcgct
      301 atggcgccct ttcgatggat gaccgccgtc aaatactttg taagttgcgt aaaagaaata
      361 tattgatgta tcgtcagttg gaacgtctga aacatgatct gcttcgtttg gagtcaaaac
      421 gtgtacagtg tgaattagag gggaaccaaa cacaggttga agtagtcgaa accaaaattt
      481 taaagaaaaa agaacaattt ttaaaaatgc ttacacaaaa taaaaaataa aggggatgga
      541 cgatggaatt tgctgagtta gttattgtat ttgcagtaac gatcacctta tcgaacatag
      601 ctagccgcat tttgccaatg attccagctc cactgatcca aattttcctt ggagtggtat
      661 taggtctgac agagtgggga caatcaatcg attttgaacc tgaattgttc ttagttatga
      721 ttattgcacc attgttattt agagaaggag aaaaagcaga tatttcttcg attctcaaaa
      781 atttcgggac aattttgttc ttggcatttg gaggagtcat tttgaccctt gtcggggtag
      841 gtgcgacgtt atcattcctg ttgccaagtg tcccacttgc tgcctgcttt gcttttggag
      901 cagcgttagg tccgacagat gcggtagcag tcagttctct ttctggacga gtgaatatcc
      961 ctaaaaaggc gatgcatatt ttggaaggag aaggtctttt gaatgatgca tctggtgtta
     1021 ctgccttcca gtttgctctt ggtgcgctga tcaccggatc tttttcagca gtaaacgcgg
     1081 ggatgagtct agtagtttct agtattgggg gagctttgat cggctttttg cttgtctggt
     1141 tcaaacaaaa aataattcat ttgatcgaaa aagcatcagc acaagatgtc acagggtatc
     1201 tgctgatcga acttttgctt ccttttctag cctatgtatt ggcagaggca tttgatgtat
     1261 ctggaatcat tgctgcagtg gcagcaggta ttctccaagc ttctggtttt aggaaaatct
     1321 ctgtgtttga tgcagaattg tcgagcttgt cccacagtac atggacaact attgctttta
     1381 cactgaatgc attagtattt atatttttag gaatcgaatt gacgcaagtg ttctcgccag
     1441 tatggggaga cggtttatat ccaaacggtc ttttattagc gattatcgta ttaatctctg
     1501 tcatgctttt tgtgatcaga ttcatttcta tcagtctttt ttatgtattc aaagatggca
     1561 gtaaaaagtt caaaaaacag ttgaacgaga tcttgatctt aacatttggc ggtgtaaagg
     1621 gaacagtcag tttggctacg atttttattt tgccattttc aatcaataat atgatgtttt
     1681 accagcggtc tttgcttttg tttctgacgg cgggtgtcat cttagtgact ttggtcatag
     1741 ggatcattgt cttgcctatg ctgacggaaa cagaggaagc gaaaagcacg gatttgaatg
     1801 ctttgatgat tttagaagag gtcgttgaag ttttgcgaaa agaaataaag gaaattgatc
     1861 gtaatacaaa agaattttta gccactgaag cagtgataga aaattatcaa gaacggatac
     1921 gtgatctgta cttggaagat ttaaccgatg atgaaaagca agaagtacaa gaaatacaag
     1981 cactgatttt gtcgatcgaa cgggatggat tggatgaaag ttatcgttcg ggcaagcttt
     2041 cttctaatgg ttatcgtttt tactcccgtt ttctttctcg attcgaacat tctatcacaa
     2101 gccagatttt gtcttttatc ggcttttggt tcattgttgt ccgtaggtta atgcgaatcg
     2161 ttctccatcc gaaaatgttt tggcaaagaa gatatgctga tcgtcagaca tttattagtg
     2221 atgaagatat ccaagaaatc agagagacat atcaaaaaaa tactcagttg attattgaaa
     2281 gtttagataa cttgactgat atttatgatg aaacgatggt tcaatttttt atccagcaac
     2341 gcaaacttga aggaataaaa atgaactcgg ggaatttgat ttcttcgtgg atgatccagc
     2401 aagatagtct gtttacgaaa aaaatgctaa gaggttatta tcttgaaaga aaaatcattg
     2461 atgaatacga agttgctgaa aaaatcacga cgttttctgc gaacagctat cgcaggaata
     2521 tcaatttgtt agaatcttat acgatgaaca aaccatctga taacttttct ttccgatttg
     2581 cgtttcgagc aaagtcaaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactaga tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcattttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacaccggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt cttgcgtttt tcttaagaat ttctcaaaca aaatgtgagg
    18781 aattgcgcca attgtgaaca aacttagaaa tattggttaa attctgacaa tttatttgtc
    18841 tttttataaa cgctgtgcta aattattctc tgggacaaaa ggaaggtgag aaaatgacag
    18901 aagccacgtg ggaacaaaaa tttttgcgtt atttgattgt cgaaagagga tattcagaaa
    18961 aaacgaaaga agcatatgaa gaagacattc tccatttcaa acattttttg gaggaatctg
    19021 gagacgctga tctgctgaag gcggaatatt ttacggttcg tgtctatttg agtgcattag
    19081 ctgatcatcg tcctagctat agcagaaatt ctatcagtcg caagatagcc agcctacgtt
    19141 cgttttatca gtatcttctc aaagaagaag tgataaaaga aaatccattt tcttacgttc
    19201 atctcaaaaa gaaaaatttg agactgccac gtttttttta tgaaaatgaa atgcaagcac
    19261 tttttgacag cgtagcagga gatagtcctt tggatttgag gaaccgcgct ttactggaag
    19321 tcctttacgg gtcaggcatc cgtttaagtg agtgtagcag tttgtcggta tcagacattg
    19381 attttgattc agaggtcatg ttgatccatg gtaaagggaa caaagagaga tatgctcctc
    19441 taggttcttt tgctcaagat gctctgcaag aatatttcag ggaaggccgc caggtattga
    19501 tgacaaaata tcacgaagag catgatatcg tttttgtgaa tcatcatgga aaaccaatca
    19561 cgcctactgg aattgaatat gtcttgaatc aaatgatcaa aaaaagcagt ttggatagtg
    19621 atatccatcc tcacatgctg cgccatacat ttgctactca tttattaaat aatggggcag
    19681 atttgcggac agtccaagaa ttgctaggac atgccaattt atcgactacc cagatctatg
    19741 cccatgtaac aaaagaaagt ttacaaaaaa actatcgttc tttccatcca agagcgtaaa
    19801 aataaggaaa agaacaaatt taggaggaaa caaacatggt cgaatcacca tttcattcaa
    19861 ccactatctg tgccgtcgaa aaagacggga aatttgctat ggctggagat ggccaagtaa
    19921 caatgggtga atctgttgtc atgaaaggca ctgcaaagaa agtacgacgt atctacaatg
    19981 gagaagttgt tgtaggtttc gccggaagtg ttgcagatgc gtttacttta gaagaaaaat
    20041 ttgaaggcaa actaaacgaa tataacggga atctacaacg cgcagctgtc gaattagctc
    20101 aagaatggcg gacacagcaa tctatgcaaa aacttgaagc gatgctgatc gtaatgaatg
    20161 ataaagaaat gcttcttgtt tcaggtacag gagaagttat cacaccagat gatggtatct
    20221 tagctatcgg atcaggaggg aattttgcgt tggctgcagc ccgagcaatg aaaaactatg
    20281 ctcaaaacga catgagtgcg aaagaaattg cagaaaatgc gttaaatatt gccgcagata
    20341 tctgtatctt tactaatcat aatattatcg tagaagaact gtaagaggtg agtagatatg
    20401 tatgaattaa aacaaacacc aagacaaatc gtaagtgaat tagatgaata tatcgtagga
    20461 cagcacgcag caaaaaaatc tgttgccgtt gctttgcgta accgctatcg ccgtttgcag
    20521 ttagatgaaa aaatgcaaca agatattaca ccaaaaaata tgctgatgat cggacctaca
    20581 ggggtcggta aaacagaaat cgcacgacgt ttggcaaaaa tcgtcaatgc accatttgtc
    20641 aaagtcgaag caacaaaatt tactgaagtc ggttatgtcg gaagagatgt agaatcaatg
    20701 gttcgggatt tagtagaaaa tgcgatccaa atcgtagaaa aagagcagta cagccgtgta
    20761 tacacaagtg ctaaaaaaaa tgcagaaaaa cgtttggtaa aaatcttggt acctggtatc
    20821 aaaaaagaac agaaacaatc cactaatcaa tttgaacaaa tgatgaatct ttttaatgct
    20881 agtcagcaaa ataccgctga accgcaagaa gaagtgacag aagatattcg aatcaatcgc
    20941 cgtacagtct tggaacaact acaaaagggt ttactggata gtcgtgaagt aaccattgaa
    21001 gttgaagacc aaaagaaacc tgcaccaatg atgaacaatg gattggagca aatggggatc
    21061 gacctaaatg aaactctagg tgctttgaca ccgaagaaaa aagtggaacg gacggtaaca
    21121 gtcaaagaag ctttagagtt attgatcaag gaagaatcag caaaattagt caaggatgct
    21181 gatatccata gtgaggcaat caaattagcg gaagaaagtg gaattatttt tatcgatgaa
    21241 atcgataaga tcacttctaa atcacagcaa tctggagaag tatccagaga aggcgtccaa
    21301 agagacatat tgccaattgt cgaaggttca caagtaaaca caaaatacgg gatcatccaa
    21361 acagatcacg tcctattcat tgcttcagga gctttccatt tgtctaaacc aagtgatcta
    21421 attccagaac ttcaaggacg cttcccgata cgggtagaat tagatgattt gacagctgaa
    21481 gattttgtga agattctgac cgaaccaaat aatgctttag taaaacaata cgtcgcgttg
    21541 atcggtacag aaaatgtgtc agttacgttt acaaaagaag caattgaacg gatcgctcaa
    21601 atcgctttcg atgtcaaccg tgacacagat aatatcggcg cacgcagact gcataccatt
    21661 ttggaaaaac tactagaaga tctattattt gaagcaccag atatgcaaat gggagaaata
    21721 acgatcacag aagcatatgt ggatgacaaa cttggctcaa ttgcggcaaa tgaagattta
    21781 agtcgctata ttttataaaa aatcggagga cgacaatgac agacttgcta acgaaaaccc
    21841 gcaagataaa cacacttttg cagcaaaaga atacttttga catgcaagca gatttgcctt
    21901 acgataaaat ggctgtcaca ctaggtggta ttcttgacag caacacctat atcataggaa
    21961 gtgacggagt attactaggt tataatgaac gtcacgatgt gaacaatgaa cgaatcaaaa
    22021 caatgtttgt tgagaaacaa tttcctgaat cttatactga tacagtggca cagttgatga
    22081 aaactgaagc taatatccca attactagtg atattactgc atttccggta gagtcaagag
    22141 agaaatatcc atttggtctg acgactgtcg ttccgatctt tggagctggc gaacgtttgg
    22201 gtacgattat cttatctcgt atggaacaat cgtttgcaga tgacgatcta gtcttagcag
    22261 aatatggagc tacggttgtc ggtatgcaga tcctttatca gaagtctcga agcatcgaag
    22321 cagatgtacg gagtgcaacg gctgtccaaa tggcaatcaa cacattatct tatagcgaat
    22381 tgaaggctgt acaagctatc tttgaagcat tggatggaga tgaaggtcgt ctgactgctt
    22441 ctaatattgc cgatagtatc ggcatcactc gttctgtgat cgtcaatgct ttacgaaaat
    22501 tggaatcagc tggaatcatt gaatctcgtt cgttaggcat gaagggaact tatttaaaag
    22561 tattgaattc ccgttttaaa gacgagttag caaaacacag ttattagaag taaggggact
    22621 ggggcagata gaaatgtttg ttccagcctt cccattttaa aaagtaagtc gaagttggag
    22681 gaatggcaat gactgttatt attcaaaatg accagctgat cgccgaaatc gctgaacacg
    22741 gtgcggaatt gatcagtctg aaatcaaaag aacatggttt agagtatatc tggcaaggtg
    22801 atccagcgta ttggggcaga catgcaccgg ttttattccc ttttgttgga cgtttaaaag
    22861 atgatcagta tgtttatcaa gggaaaacat atcaaatggg gcaacacgga tttgctcggg
    22921 atatggattt tgaagtcatt gaacatggac aagaaatggc tagtttcatc ttaaggagta
    22981 cgccggagac attagaaaag tatccatttg attttgaatt agtgatcagc tatgaattag
    23041 gaggagacgg catcacggtt cattatcaag tggcaaatac cggtgaaaaa gaaatgttct
    23101 tttctatcgg tggacatcct gctttcaatg tacctcttga agaaggattg tcatttgaag
    23161 atttctatct ggcattttct ccattgaaat caagattacg cctaccattg aaaggcccat
    23221 tcatcgatat ggatcaaaag acgttaggtc agacaaatac gaatctggct ttgaatcgtg
    23281 aactttttga gcaagatgcc ttggtttttg aaacaagggg tctaaatgcg tacgccattc
    23341 gaagtgaaaa atcttcccat agcgtaactt taagctataa agatatgcct tatgtaggga
    23401 tctggtctcc ttatccaaaa gaagcgcctt tcgtttgtat cgaaccgtgg tgcggaatcg
    23461 ctgacacggt gaacagtagt ggaaacttga tggaaaaagt agggatcaat caactaggtg
    23521 cccatgaaat cttcaagaca aaatattcca tcacagtgaa gtaagaggtt gtgaccgaga
    23581 agttagcagg gaacaccgtt taccaaggtg tgtaaaaaag ctatcctgta agtaataagg
    23641 gagtgtgaca tcgattt
//
LOCUS       DAFFSV010000035        23216 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFFSV010000035 DAFFSV010000000
VERSION     DAFFSV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.035"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            54..1379
                     /gene="ftsA"
                     /locus_tag="LR110_001231"
     CDS             54..1379
                     /gene="ftsA"
                     /locus_tag="LR110_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBL4822203.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            1402..2643
                     /gene="ftsZ"
                     /locus_tag="LR110_001232"
     CDS             1402..2643
                     /gene="ftsZ"
                     /locus_tag="LR110_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBL4822204.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            2660..3337
                     /locus_tag="LR110_001233"
     CDS             2660..3337
                     /locus_tag="LR110_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBL4822205.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            3351..3950
                     /locus_tag="LR110_001234"
     CDS             3351..3950
                     /locus_tag="LR110_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBL4822206.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            3965..4252
                     /locus_tag="LR110_001235"
     CDS             3965..4252
                     /locus_tag="LR110_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBL4822207.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            4301..5083
                     /locus_tag="LR110_001236"
     CDS             4301..5083
                     /locus_tag="LR110_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBL4822208.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            5104..5811
                     /locus_tag="LR110_001237"
     CDS             5104..5811
                     /locus_tag="LR110_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBL4822209.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            6073..8871
                     /gene="ileS"
                     /locus_tag="LR110_001238"
     CDS             6073..8871
                     /gene="ileS"
                     /locus_tag="LR110_001238"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBL4822210.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(8920..10446)
                     /locus_tag="LR110_001239"
     CDS             complement(8920..10446)
                     /locus_tag="LR110_001239"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBL4822211.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(10461..11108)
                     /locus_tag="LR110_001240"
     CDS             complement(10461..11108)
                     /locus_tag="LR110_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBL4822212.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(11292..11621)
                     /locus_tag="LR110_001241"
     CDS             complement(11292..11621)
                     /locus_tag="LR110_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBL4822213.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            11798..12526
                     /gene="sfsA"
                     /locus_tag="LR110_001242"
     CDS             11798..12526
                     /gene="sfsA"
                     /locus_tag="LR110_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBL4822214.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            12542..13555
                     /locus_tag="LR110_001243"
     CDS             12542..13555
                     /locus_tag="LR110_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL4822215.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            13555..14832
                     /locus_tag="LR110_001244"
     CDS             13555..14832
                     /locus_tag="LR110_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL4822216.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            14895..17597
                     /locus_tag="LR110_001245"
     CDS             14895..17597
                     /locus_tag="LR110_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL4822217.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            17749..18066
                     /locus_tag="LR110_001246"
     CDS             17749..18066
                     /locus_tag="LR110_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4822218.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            18096..18416
                     /locus_tag="LR110_001247"
     CDS             18096..18416
                     /locus_tag="LR110_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL4822219.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            18524..19984
                     /locus_tag="LR110_001248"
     CDS             18524..19984
                     /locus_tag="LR110_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4822220.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            20052..20273
                     /locus_tag="LR110_001249"
     CDS             20052..20273
                     /locus_tag="LR110_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822221.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            20304..20486
                     /locus_tag="LR110_001250"
     CDS             20304..20486
                     /locus_tag="LR110_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBL4822222.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            20486..20899
                     /locus_tag="LR110_001251"
     CDS             20486..20899
                     /locus_tag="LR110_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBL4822223.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            21022..22203
                     /locus_tag="LR110_001252"
     CDS             21022..22203
                     /locus_tag="LR110_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4822224.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(22274..22438)
                     /locus_tag="LR110_001253"
     CDS             complement(22274..22438)
                     /locus_tag="LR110_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822225.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            22592..22665
                     /locus_tag="LR110_001254"
     tRNA            22592..22665
                     /locus_tag="LR110_001254"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22626..22628,aa:Arg,seq:cct)
     gene            complement(22734..23009)
                     /locus_tag="LR110_001255"
     CDS             complement(22734..23009)
                     /locus_tag="LR110_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL4822226.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT         7627 a         4009 c         4920 g         6660 t
ORIGIN      
        1 gacgctagtt gttcgcttgt atatggaata gtaagtagga gggaatccca ttcatggcaa
       61 aaacaggaat gtatgtaggc cttgatattg gaacgacatc tgttaaagtt gtcgtggctg
      121 aatatatcga cagccaaatg aatattattg gagtaggaaa cgcaaaatca gaagggatta
      181 accgaggcat tatcgttgat atcgataaaa cggtccaagc gatacaacga gcagtacgac
      241 aagcagaaga aaaagcaggt atccagatca aaggcgtaag tgtcggtttg ccagcaaaca
      301 tgcttgaagt agaaaattgc cagggaatga ttgcagttaa cggagattca aaggaaatca
      361 ccgatgaaga tgtcagaaat gtggcttcag ctgcccttgt tcgttcaata cctcctgaac
      421 gtcaaatcgt gtcaatattg ccacaagatt ttactgtcga tgggttcgag ggaatcaaag
      481 atcctcgtgg aatgatcggc gtaagattag aaatgtacgg tctgttgttt acaggaccta
      541 aaacgatcat ccataacata cgtaaatgcg tggaaaatgc cggattaatc gtcaacgaaa
      601 tggtgattac tcctttagcg ctgactgaat caattctttc tgacggagaa aaagattttg
      661 gtacgatcgt cattgatatg ggtggcggcc aaacaacaac ggctgttatg catgataagc
      721 aattgaagtt taccaatctt gaccaagaag gtggcgaatt tgtcactaaa gatatctcta
      781 ttgtattaaa tacgtcattt aacaatgcag aagcgttgaa aataaactac ggggatgcat
      841 acccagaacg gacatcacca gatgaagaat ttccagtaga tgtgattggt cagtcagaac
      901 cagtgaaagt cgatgaacgc tacttgtcag aaattatttc agctcgaatg gaacagattt
      961 tcaacaaagc aaaagatgca ttggaccaaa tcgaagcatt agagttgcca ggtggcgtcg
     1021 tattgacagg cggagctgct agtcttcctg gtgttgtgga cttggcacaa gaaatcttcg
     1081 gcgtaaatgt aaaattgtat gttccaaatc acatggggct gagaaacccg gtatttacaa
     1141 atgtaataag tatcgtggat tattcagcaa acttgagtga agtgtatcaa ttggcaaaaa
     1201 ttgctgttac gggtgaaacc tcagcagctc gtcaaatggt agttgaacaa gaaacaacaa
     1261 acacatatga gagctatgaa gcacctgaag aaacaatcta cgatgagccg gaaccaaaag
     1321 agtccggaga aaatgtaaaa aataaaatca agggtttctt tacaaatatt tttgattaat
     1381 tcatcctggg aggaataaga tatggaattc tcaattgata ataatatcaa tgacggcgca
     1441 gtaatcaaag tcatcggagt cggtggtggc ggtggaaatg ctgtaaaccg aatgattgaa
     1501 gaaaacgtta aaggtgttga attcattaca gccaacacag acgtgcaagc attaaaaaat
     1561 tcaaaagctg aaacagttat ccaattaggc cccaaatata ctcgtggttt aggtgctggt
     1621 tctcagccag aagttggaca aaaagcggca gaagaaagtg aacaatcctt acgcgaagct
     1681 ttagacggtg cagatatgat tttcatcact gcaggaatgg gtggaggaac cggtacaggt
     1741 gctgcaccaa tcgttgcagg tatcgcgaaa gaattgggcg cattgactgt tggtgtcgtg
     1801 acacgtccat tcacttttga aggaccgaaa cgtggccgtt ttgctgcaga aggtatcgct
     1861 cgtttgaaag aaaatgtgga tacattatta attatctcaa ataaccgttt attagaagtt
     1921 gtagataaaa agacaccgat gcttgaagct ttccgtgaag cagataatgt tttacgacaa
     1981 ggtgtacaag gtatctctga tttgatcaca gcgccaggtt acgttaacct tgactttgct
     2041 gacgtgaaaa cagtgatgga aaaccaaggt acagcattga tggggatcgg tgtggctagt
     2101 ggagaagatc gcgtagtaga agcaacgaaa aaagcaatct cctctccatt gcttgaaaca
     2161 tctattgatg gagctgaaca agtacttctt aacatcacag gcggtttgga tatgacattg
     2221 ttcgaagcac aagatgcttc agatatcgta gcaaatgcag caactggcga tgtaaatatc
     2281 attctaggta catctatcaa tgaagaaatg ggcgatgaaa tccgtgtgac ggtcatcgca
     2341 acaggtatcg atgaatcaaa aaaggaacgc aagtctagcc gaccagctag acaagcacaa
     2401 atgcaatcac ctgctcaaaa gactgtatta gatatggatc aagctaaacc aacttcttca
     2461 gaagaagaaa acagttttgg cgattgggat atcagacgtg aacaaaatgt tcgtcctaga
     2521 gttgatgatt ccaattttga caatatcgaa aaaaaagaat ttgatacatt caaccgcgaa
     2581 gaaacaaaat ctcatggcga tgatgaatta aatacgccac cgtttttccg tcgtaaaaaa
     2641 taaaggagag gaagagcaca tgattgctga caacttgcag aaaatcaagc aagaaattca
     2701 ggattcgtgt gctcttgttc atcgtgatcc gaaagaagtt acattagtag ctgtaacaaa
     2761 atctgttgaa tccgctcaag ctgaagaatt ggttagagct ggcgttatga attgtgcaga
     2821 aaaccgagta gataaattat tagaaaagaa acaagattta tttgattttc ctgaaataaa
     2881 gtggcattta attggtaatt tacagcgcag aaaggtaaaa ttgattgtaa atgaaattga
     2941 ttactttcat gcgttggaca gtttaagatt agcagaagaa atccagaagc ggttagagaa
     3001 agaattatcc tgttttattg aagtaaatgt ttctggtgag gaatcaaaac atggaatttc
     3061 accagatgag cttttaccgt ttgtggaatc tttagctgcc tgcacaaaaa tcaaggttgt
     3121 tgggttgatg actatggcgc ctaaagaagc ttctgatgag caaatccata aagtgtttgg
     3181 tacgctgaaa gaattgcgtg atcttgttca agcaaaaggt tatgcccatg caccatgtag
     3241 tgaattaagt atggggatga gtcaagactt tcaaattgcg attgaagaag gtgcaacatt
     3301 tatccgtgta ggaacagcgc tattcaaaga cgaataaagg aggaacaaaa atgccgcttt
     3361 taaacagagg agcaattgct aattttttcg gattagctga tgaggaagag tacgaatacc
     3421 aagattatcc tgaaacaaca aaaaaggctg tacagccaac taaaaaaaat acaacacaga
     3481 atcaatcaac tcattctgta tctgctgcga atactcatga aaaacaaaaa acggcgactt
     3541 cacgtcctgt aacgcatgaa agtattcagc cggctgaaaa gaaaaaagta attgaactac
     3601 atcaatcacc tacaaaaact gtagcgaaag aaagaacagc ttcaagaacc tctacacaac
     3661 ctaaagcaaa tcttgctgga aaaatcactg taatggaacc aagagcttat tctgaagcta
     3721 tggcgattgc taaacgaatc attgctggag aagcaacctt agtgaatttt catttagtag
     3781 aagaaaaaca agcgagaaga atcgttgatt ttctaacagg aacagtttat gcactagatg
     3841 gagatatcaa acgtgttgga gatgagattt ttctttgtac accgactggg atggagattg
     3901 acagttcaac cgctcaatct tttgcagatg cgaatttgtt tgatctgtaa gaggaggaac
     3961 tacgatttat actattgcac tattacttca tttacttcaa cgggcagcct atttctatag
     4021 cattatcttg atggtatatg cattgctctc ttggtttcca ggcgggtatc aatcagggct
     4081 aggacgatta ttagctaaga tctgcgaacc gtatatcagt ttgttcgatc gtctgccttt
     4141 gaggattgga cccttggatt tcacaatttt aggggcaatc ctgatcttga acttagccgt
     4201 acaaggttta gcacagatcg ttatatggat aactgtccat ttagtttatt gattgaagca
     4261 acaaaatctt tattctcaga atagatagga ggggaaaatt ttggatgcaa atgtatacca
     4321 gcactttcgg gcagatgaac gtacatttat cgattcggta ggcgattgga ttgaacaggt
     4381 tcaaggacaa tatgcaccct atctgaccga atttcttgac ccaagacaat cctatatttt
     4441 agaaacactg attcgacaaa gcagtgattt gcgtttcatg ttttatggcg gatatgaaca
     4501 ggcagaaaga aaaagatgtt tgattttccc cgattattac gaacctgtag aggaagattt
     4561 tgaaacagca ttgtatgaaa tccattatcc tagcaaattt gccagcctaa gtcatgggaa
     4621 aatccttgga actttagtgg gaacggggat caaacgctct tactttggcg atatcatctc
     4681 ggacggtgat cgctggcaag tatttattgc aaatgaggtg gatcattttg ttgctgcaca
     4741 agtaacaaag attggaaagg ttgctgtacg cttggaacca atcaaataca cagaaatgat
     4801 tatgccgaaa gatagctgga cacaagaaag aggaacagtt agttccatgc gactagacag
     4861 tgtgatctct gagatttata atatttccag acagcggtcc aagcagctga tcgaagctgg
     4921 taaagtaaaa gtgaactgga cagaaaatac aagacctgat tttgtacttg aactattaga
     4981 tattatttca gtcagaggtt atggcagagt ccagatccaa gaacttgagg ggaagacgaa
     5041 aaaagaaaag tacagggtac tgttcggtgt attacgtaaa taaaataaaa aggtggttta
     5101 ctcatggctt taactccatt agatatccaa aataagacat tcccgacaaa aatgcgggga
     5161 tataatcaag acgaagtaga tgatttctta gatttagttg ttcgtgacta tgaggaattg
     5221 actcaaagaa atcgagaatt agaaaaagca gtcaaacatt ctgaagaaaa acttgagtac
     5281 ttcaatgaat taaaagatgc tttgaaccaa tcaatcatcg tagctcaaga tacggctgat
     5341 aaagtaaaaa caagtgcaag caaggaatcc gaagtgatcg ttacttctgc acaaaacaaa
     5401 gcagatgagt tagttgcgaa cgctgaaaaa cgtgcacatc aattaacaac tgatgcagaa
     5461 gaaaaagcaa gaaaaatctt gacagatgca acagaaaaag caagacagct tgcgacagaa
     5521 actgaagatc ttaagaaaaa aacacgagta ttccatcaac gtatcagctt gatgcttgag
     5581 tctcaattag aacaagtgaa aagtccggaa tgggatgaaa ttttacaacc attctccagc
     5641 tacgtgactg acagccatga agtgatcaaa gaagttttag caaaacagct tgacaatgaa
     5701 aatgatactg atgtaaactc agacacagga gtcatagaag cacctatgac ggcatttgat
     5761 acacaggcta ttgatatctc gatcgtgcct ggtgaaaatg aaaacgagta gaacagaaaa
     5821 gcactttgtt attcttaaag cgagccgatg agagtggaag ttcggtaaga cactgcatgg
     5881 tgaaagatcc tctacttgag acctgctttt ccgaaattag agtaagaagc agcggctgac
     5941 ctcgttattg gtcacagaga gtttgcacta gatagatgca actaaatttg ggtggtacca
     6001 cggcatgttc gtcccttgcg gctgaacatg cttttttgtg tccggaaaac aaataaagga
     6061 gtgtatggtt gaatgaaaat gaaagaaacc ttgcaattag gaaaaacaaa attccctatg
     6121 cgtggtaatt tgccaaaccg agaaggcgag tggcaaaaag aatgggaaga aaatcgaatc
     6181 taccaaaaaa gacaagagtt gaacgaagga aaacctagct ttgttcttca tgatggacct
     6241 ccatatgcca atggtaatat ccatttagga cattcattga ataaaatcag taaagacatt
     6301 atcattcgtt caaaatcaat gtctggctat cgtgcaccat atgttccagg atgggataca
     6361 catggattgc cgattgaaca agtattggct aataaaggcg tgaaacgtaa agaaatgtcc
     6421 atggcagaat acttggaaaa atgtcgtgaa tatgcacttt cccaagtaga taagcaacga
     6481 gcagatttca aacgcctagg agttgcagga gattgggaac atccttatgt tacattagat
     6541 ccttcttatg aagctgcaca agtacgcgta tttggtaaaa tggctgaaaa aggctatatt
     6601 tacaaaggat tgaaaccaat ttactggtct ccgtcaagtg agtcttcttt agcggaagct
     6661 gaaattgaat ataaagatgt aaaatctcca tctatttttg tagctttcaa tgtcaaagac
     6721 ggcaaagata ttcttgatga agatacagct tttgtcattt ggacgacaac accatggaca
     6781 ttgccagcaa accaaggtat tgctgtaaat ccaaaattta cgtatgtatt ggtggaagcc
     6841 gatggccgta agtttgtcgt agcgaaagat ttgctagaaa cagtccaatc agaaatcggt
     6901 tgggaaaatg taacagttct aaaagaattt gctggacaag aaatggaata tatgactgca
     6961 agtcatccat tctatgaacg tgaatcactt gtgattttag gcgatcatgt tactttagac
     7021 gctggtactg gattagtcca tactgctcca ggacatgggg aagatgacta tatcgctggt
     7081 agcaaatata aactatctgt agtgtcacct gtagactcaa aaggattgtt tacagaagaa
     7141 gcacctggat ttgaaggtat cttttatgac aaagcaaatc ctatgatcac tgatctatta
     7201 aaagaaaaag gagctttgct gaagctagat ttctttactc atagttatcc tcacgattgg
     7261 cggacgaaaa agcctgtgat ctatcgggca actccacaat ggtttgcatc tatcgataaa
     7321 ttccgtcaga atattttgga tgaagtagaa aaagtagagt gggttatccc atggggtaaa
     7381 acacgtttgt ataatatgat ccgtgaccgc ggcgattggg taatttcccg tcagcgtgca
     7441 tggggagtac cacttccgat tttctatgct gaaaatggag aagcaatcat cacaccagaa
     7501 acaatcgaac atgtggcaaa attatttgct gaacatggat caattatctg gtttgaaaaa
     7561 gaagcgaaag aacttcttcc tgaaggattt acccacccag gttctcctaa tggcgaattt
     7621 acaaaagaaa aagatatcat ggatgtctgg tttgattctg gttcttctca tgaagctgtt
     7681 ttacgtcagc gaccagaatt atcattccca gcagatatgt atttagaagg atcagaccaa
     7741 tatcgtggct ggttcaactc aagtatcacg actagtgtgg ctatcaatgg tgtagcacca
     7801 tacaaatcag ttatttctca aggatttgct ttagatggtg aaggccgtaa gatgagtaaa
     7861 tctttaggaa acatcattgc accagaaaaa gtgatcaaac aatttggtgc agatatcctt
     7921 cgcttatggg tatctagtgt agattatgaa gcagatgttc gtgtttctat ggatatctta
     7981 agtcaagtgg cagaagtata tcgtaaaatc agaaatacga tgcgtttctt attagcgaat
     8041 acagaagatt tcgatcctaa aaaagatact gtttcgtaca atgatttacg ttctgtagac
     8101 aaatatatga ccgttcgatt gaaccaagta atcaaagaaa tccgagaaga agggtacgac
     8161 aaatataact tcatgcatat ttaccgtaca gttatgaact tcttgacggt tgatctatcg
     8221 tcattctacc tggattttgc taaagatgta gtttatattg aagcagaaga tgattatcaa
     8281 cgtcgttgca tgcaaactgt tttctatcaa acagctgtag ctttgacaaa attattgact
     8341 cctatcattc cgcatacagc agaagaaatc tggtcattcc tgaaagaaga agaggagtac
     8401 gttcagttaa gcgagtttcc tggttatgaa gagtttgcaa accaagaaga attgatggat
     8461 acctggactg ctttcatgga cttccgtgat aatgtcttaa aagcattgga agaagcaaga
     8521 aacagcaaat tgattggtaa atctttggaa gcaaaactaa ctgtttatcc aaaacaacaa
     8581 gttcgtgaaa tgttaaaagc tttggatgca gatattgcac aattattgat cgtttcacca
     8641 gactactttg aagttatggc agcagatgaa caagtgccag ataacgctat ggtatttgat
     8701 gatgttgcta ttacagtgga aaaagcagac ggggaaactt gcgatcgctg tcgccaagtt
     8761 cgtaaagatg taggagtaga tgaaaaactt cctcatctat gcggtcgttg tgcaaaaatc
     8821 gttgaagact tctatcctga agctgtagct gaaggattcg aagaaaagta aaagggaaaa
     8881 gggggtgtga catgttcttt gtcacacccc cttttgttat caatcgttta attttccacg
     8941 ttcacgatac cagttatctg gttgccagat ccattcgaat ccgtcttttt caagtagttc
     9001 gaaagctgct ttaggtccca ttgatccagc tttgtagttg ggaaaatccg gttgtgtttg
     9061 gtcccaagct tttcgaatga catccacgat atgccaagat tgagcgactt cttcccaatg
     9121 agagaagttc gttccatcac cattcaacgc atctaatagt aatttttcgt aagcttctgg
     9181 gctattttca accatttctg cactatttct aaattccaat ttgattggtt ccgtttcaaa
     9241 tccttgccct acttctttcc cattcaagga taaagaaaat ccttcagttg gctgaatata
     9301 gatcgttaga acattaggtg gaagtgtcgt atcgtcacat ggttcatcga cagatgtttt
     9361 gaatacatta acaggaactt gtttgaatac gatgttgatc cgagtccctt tttcagtcaa
     9421 acgtttgcct gtacgtacat agaaaggaac acctgaccaa cggaagttat cgatagtgaa
     9481 tttgcctgcg acgaatgttt cagtctctga ttgagcatcc actttgtctt cttcgcgata
     9541 gcccttgaat tgtacaccat ccaattcacc ttgagcatat tgcccgcgaa cgaagttttc
     9601 taacacttct tcttcgttat aaatgcggat agctttcaat gctttgatct tttctgtacg
     9661 gatttctttt tcggaaaatg ctactggcgg ttccattgca agtaatgcta caacctgtaa
     9721 aatatgattt tgcaccatgt ctttaagtgc gccactatga tcgtagtagc ccccgcgatc
     9781 ttctacaccg aggttttcgc caaaagtaat ttggatattg tcgatatagc gattgttcca
     9841 ttgagcctca aaaatattat tggcaaaacg gatagctgag atgttttgga tcatttcttt
     9901 tcctaaataa tgatcaatcc tgaaaatatc ttgttctggg aatacttctc tgatttgctg
     9961 gttcaattca aaagcagact ggtaatcaga accgaacggt ttttctatga tcaaacgatt
    10021 gaagccattt tttgtcataa tattttgtga tttcaaatga gaaacgatcg ttccaaaaaa
    10081 ttgaggtgcc atagccaaat aatagacacg gttaccgttt aattgatagt gtccatctaa
    10141 tcggtcagac aattctttta atgtctgata atgcgttgaa tcatttacat catgggattg
    10201 atagtaaaaa tggcttgcaa aggtagttgc ttcttcatct gaaggattca atgcacggat
    10261 tgtttctttg acaacttcac ggtagtgctc atcgctccag ggacggcgag cggttccgat
    10321 tacagcaaaa tgctcgccta aatttccttt tctgtaaagg cggaacaaag acgggtacaa
    10381 tttacgctgg gctaggtcac ctgtaccgcc gaatatcgta aacaatacat ttttttcatt
    10441 agacatgtgg atacactcct ttatatttta tcgacaaaaa tcgtgctggc cgccttaaag
    10501 ctgacgggga ttttctttct atcattttgg atagtgatcg gtccttcata agctgcgatg
    10561 tcaacgatct catattcttc gttgatattc aggtcaatag tgactaagta atccaacaat
    10621 tctttttcat caagcactcg tgcgatacgc acttttgtac cgacaggata ggactccaat
    10681 gtttgtcggc gttcttcatg gacaacttcc tcttttttag gaatcgatcc tccatgagga
    10741 caatgttcag gatgattcaa atagttttct aaatgatctg caagatgttc cgaagtcaca
    10801 tgttccaata cttcagcatc atcatggact tcattccagc tatagtctaa atgttcaact
    10861 aaaaaaactt cccataagcg atgtttgcga atcagactgc tagctttttg cagtccgctt
    10921 gcagtcaatt gtacaccttg ataaggggaa tgttctacaa gctcttcttt cacaagttta
    10981 gaaatcattt cactgacaga tgcagctgag acatctagac tagatacgat ttgtttattg
    11041 ttgactttcg ttgtatctcc acctagttct aaaatgattt tcaaataatc ttctcgattt
    11101 ggtgtcatat tcgatcatcc tttcatggta aaaaaccatc attagtaagt gtaacaaaaa
    11161 aaacgttaat tgactatttt tctaaataaa aaacaaagga tttttagacg tttttacaac
    11221 aaaaaacgaa agatgataga gaagactcat tctttcgttt ataaaggata tatatataca
    11281 ttgggaggga tctatttttc agataacaac ttatatgaag tgtctgcaga ttcgtcataa
    11341 agagtttgtt cgggataaga aaagataaga taaaaaccat actgatcacg tacgactaaa
    11401 cgatattcat ctatttctac cacagatgtg ccaagagact gaccgttgat caaaaacgtg
    11461 taatcagatg taatctgtaa ttgttgtttt tgctggctat cactaaagaa ccatgtacct
    11521 tgatactttg agagtccttc tgacttttcc gcttgttttt tctttttcaa gtaaataaaa
    11581 cttgagagaa cggtaatgag tgaaccaaga aaatagatca ttttttttat caccaaatca
    11641 ctcctttatg agaaaataat cgcatagaca tattctttct tctttatttc attgtaccga
    11701 ataaaagcta aaatttctct aagactttcg actggttttt ttactgtttt tctacaaatt
    11761 atgataggat acatagtagg aagaaaggtg acataatttg aagtatcagc aagtggaaat
    11821 agcttttttt attgaccgcc cgaatcggtt tatagcacat tgtatgaaaa aagacggaga
    11881 aataattatt gcgcatgtca aaaatacagg tagaggaaaa gaggtcctga taccaggcgc
    11941 tgaagtggct attgtatttg ctcctggacc taaacggaaa accgcctatg atctgattgc
    12001 tgtaaaaaag aagggtgact ggtttaatat cgatagtcaa ttgcctaatc ggctggcaat
    12061 tgatggtatt ttagacggca cgatcctttt accaggatta actggtccat ttacggtata
    12121 caagcgtgaa gtaacttatc ttcactccaa atttgatatt tatttagaga cggtggtcgg
    12181 acaaaaagca tttgttgaag tcaaaggtat gacacttgaa aataaagcga ttggtgcttt
    12241 tcctgatgcg cccacactca gaggattgaa acatatagga gaattaacgt atgccgcaca
    12301 agatggttat gccgcttatg tcttgtttat tgctcagttc gaacatcttc atctggcaac
    12361 gatccatgaa gaaatgcaac cagcactagc tgatatggtt cgacatgcgc agcaaagcgg
    12421 agttcaaatc ctcgcctata attgtcaagt atcggcagat gaggtaactg tcaagcaggc
    12481 cattccattt gatgtagata tcccttttga agatcctata caatagaaag aaggtaaaat
    12541 aatgatcaac ttaggtatta tcggaacaaa ttggattact catcaatttg tccaagcagc
    12601 tttaagcaca aaaaaatatg aattgacagc ggtatattct agacatttgg aaaaagcaca
    12661 ggaatttggt tctcattatg aaggggatat cgaattcgca acagatttga agactttttt
    12721 caatattact catatggaca ccatttatat tgcttctccg aatagtcttc actttagtca
    12781 agctaagcaa gcaattttgg caggaaaaaa tgtgatagtt gaaaagccag ctttttcgac
    12841 gccaaaagaa atggatgaaa tcatccattt ggcaaatgaa cacaatgtgt atttttttga
    12901 agcagcaaga aatatccatg aatcaggttt taaaaagatt gccgattact taccattaaa
    12961 agacgagata atcggtgcaa atttcagcta tatgaaatat tcttcgcgct atgaccaagt
    13021 attagatgga gaagaaccga atatcttctc gactcatttc tcaggtggtg cattgatgga
    13081 tttaggtgtt taccttgtat atgcagctgt tgcttggttt ggaatgccaa aagaagtcca
    13141 ttatttccca agaaagattt ctactggtgt agatggtctt ggatggggga tcttacgtta
    13201 tgagaatttt gatgtatcga tccagccagg taaaatcggt gattcttatt taccgtcaga
    13261 gatttatttt gactcaggaa cgctagtgat gaatggagtc aatgcaattg aacaagcaca
    13321 atttcatgat cgcgcgcata aagaaatcat tgatttggcg gtaacagctg cagaaaatcc
    13381 aatggcagat gaagcagagg attttgccaa tgtcatggct catccgaaag atccagcttg
    13441 gggattgttg tatgaagagt ggatcgagct agctagaaat gtgaaccaag tgatttatga
    13501 tttaagaaaa aatggcggca tcaaattcga tgcagataat gaggaagatt tttaatgaac
    13561 gaatttgtac aagtatttcc cgaaaattgg cagaaaaaat atgaggataa agggtttatc
    13621 tcaccttctc ttattcaacg agcagttttt cagccactaa gtaataaaca aagtatagta
    13681 gcggtttctc ctacaggttc aggtaaaaca ctggcttatc tttggcctct actactaaac
    13741 gtggaaccag gtgaagcaag tgcgttggtc atttttgctt cttctcaaga actagctatt
    13801 caagtagcag atgttgcacg tgaatgggga aaagacaaag agttgaaagt ccaatctttg
    13861 gtgggcggag ccaacgtcaa acgtcagatt gagggtttga aggaaaaacc agaaatcttg
    13921 attggtacac caggaagaat attggaattg atgaaagcaa aaaaaataaa agcacatcaa
    13981 gtgaaaacaa tggtatttga tgaagcagac cagctatttg acgctgggaa cagccaaatc
    14041 attgatcaaa ttcttcatca agctccaaca gagtatcagc tagctttttt ttcagcgaca
    14101 gcagatcgtt cccttgaaag cattgaaaaa atcactggga aaacgtttga gacaattgac
    14161 gtgacagcag aggacgattc tcgaaaagga ctgagacatt actttttacg tgtaccgata
    14221 cgaaagaaag atgactactt gagacgtctc acgcatatag aagattttca tggtctgatt
    14281 ttctttaatc aactgactga attaggcgta atggaagaaa aattgctgta tagaggtgta
    14341 ccggtaggaa gtctggcctc tgatcagaac aagctactcc gaaaagcagc gctagaacaa
    14401 ttcaaaaaga aaatggtcag agcacttttg accacagatg tggcggctag aggtttagat
    14461 attacaggtc ttccttatgt agtgaatgca gaagttccgt taagtgaaga agcttatttg
    14521 catcgttcgg gaagaactgg tcgaatgggt aatgaaggaa tagtaattac actcgtacaa
    14581 gataataatc taagagattt aaaacggctt ttacgtccat caaatataac acttgaagag
    14641 atttttttat acggaggtaa gctgcaaact gagcagccaa ccaaagaaga tgcagctcct
    14701 gcagtaaata aaaagtatgc gaaaactttt tctggaaagg agcagaaccc agaatataac
    14761 aaagaaaaaa taagaaaaaa agttaaaaat aaatcaaaaa aagagagaaa taaaggagca
    14821 aggcgcaaat aagcgccttt ttatgctatg attatgaaaa cgggaacaaa attggttgcc
    14881 agaggagggg ttatgtgaaa gaacgtcaag aagagatctt gcagctattg acagaaaacc
    14941 aacaaggatt gacagcaagt gatgtagctg agcgcttaac tatcgatcga agcaatgcaa
    15001 gccgatattt aagtgaacta tacaaggccc atcatattgt aaaaaccgct ggccgaccag
    15061 tcgtttactc tttgccaacg gaaaagtcaa aatctgatga agtgcatgtc gactcttcta
    15121 cgcaagtgac ttttgaaaca ctcgttggag aaaatgattc tttaaaagtc agcatacagc
    15181 aggcaaaagc tgcgattctt tatccaccgc gggggttgca tacgattatt tttggagaaa
    15241 ctggtacagg gaagtcgatg tttgctgaat gtatgtatca ttttgcaatc gattcagaga
    15301 tgctttctgc agatgcgcca tttgtttctt tcaattgtgc ggattatgct caaaatccgc
    15361 aactgctctt tggacacatc tttgggatca aaaaaggggc ttacacagga gcagctcaag
    15421 atagtcccgg tctaatagcc aaagcagacg gcggtatatt gtttttagat gagatccacc
    15481 gtttaccgcc ggaagggcag gaaatgctgt ttactttcat tgataaaggt atttaccgtc
    15541 cgcttggaga aagtgcacaa gtacatgaag catctgttca gatcattggg gcgacgactg
    15601 aatcttcaga aagtttctta tctacgttca atcgacggat tcctatggca atcaccttgc
    15661 cgaatcttgc ttcacgttca ctggatgagc gttatgaaat catctctctt tttatcaaac
    15721 aagaagcgaa tcgtttgaat caacgtatcg atatcgaaaa agaagcgatt cttgccttca
    15781 tgctttatga tgcggaagga aatatcggtc aagtgaaacg agacttgaaa ctagtctgtg
    15841 caaaatcttt tttacattac cgtactcatc atgagaaaaa gctaatcatt cgaaaagaag
    15901 aactttccct tcaagtacaa aaaggattac tgaaaatcaa ggaaataccg gaacgtctcg
    15961 accgattcat ggacagcaaa agtcagtatt tgacatttga accaggattt gctgatgttg
    16021 tctggtcaca agatccagaa cgaaacatgc aggtctataa cgatattgag gaaaaagtca
    16081 cttctctttc cgaaaccggt gtggagaaca ttgatctaga gaccctcatt tcaaaagatg
    16141 tggatgctta tttccagaca tatgtcaaag aattgactaa aagttcgatt cccaaagatc
    16201 ttttgccaga tgaaatctgg cggttaacca atcaattgta tgatgtggct gaaaaacggt
    16261 tagaccgtat ttataatgaa aaagctcgtt ttgcttttgc tttgcatctg caaagtacat
    16321 tggatcgtgt gagagaaggt catatgatcg tccacccaga tttgaataac gtacggaaaa
    16381 attttaagag ggaattccag gtagcaatcg atctttcaac gatcattgaa gaagaagaac
    16441 aagttgagat tccttttgat gaaatcggct ttattagtat gtttctttct atcaatctag
    16501 gagaaagtga gcctgttaaa gaaaacaaag tagatgttct catattgatg cacggcagac
    16561 agactgcttc aagcatgttg gagatggcac aagaactatt aggaacaaaa gtagggaaag
    16621 cttttaatat gccactggtt atggaagttc aaacaatgta tgaagaagtg ttacaatatg
    16681 tcaaaaacta tcaagagcat ttgacgaatg gcctactctt gttaacagat atgggatcat
    16741 taaacacctt cagtgatttg atttatgaag agactggcat tcggacgaaa gctatcacga
    16801 tgacaagtac catgattgtg atagaagcga tacggatggc aagtgttgga agaagcttag
    16861 aagatattta tcaaaatatc cagctttcct ttgaatcgat cgtacgtgat cagttcaaag
    16921 cggaaaagtc tgaaacttta cagcgtaaaa aagccgtaat cgtgacatgc tttactggtg
    16981 aaggagtagc tgccaaatta tatcagcgta ttatgccagt gattaatcaa aataaggtag
    17041 aactgatcca gatgcagttt ttagaacgtg aatcattcaa aaaacacatc gatgccttat
    17101 tggaagaata tgagatcaaa gcaattgcgg gaacagttga tgttgattat caaaatatcc
    17161 catttttttc agcctatgac gtttttgatg atgaaaaact aaatatttta aagcgtatag
    17221 ctgatgatga gatcccgaca gctaaaattg tcgattcact tgctggatca ttaagcgatg
    17281 tcttttctat tgatgaactg atccgttcat tgcaaaagac agttcagcag atccagaccg
    17341 atttgtcgat tatcgtggaa cctggagtag atactggaat cgttatccat ttagccttca
    17401 tgttagacgg attgatcaaa caagaaagcc atcggcaatt tgagaaactg tctattttct
    17461 caaaaattta tcgtttggaa atggatatcg ttcgggcaaa cttgatggca ctagaacgaa
    17521 aatatcaggt aaaaattcca gaagatgata tcgcatttct tacacaaatg tttcttgaaa
    17581 ataaaataaa aaaatgaaca cactaatcat actgtttaca cactaaacat ctgtgtgcgc
    17641 actataaaaa agagctaaaa agcatgaaaa cgctatttca gctctttttt ttattggcat
    17701 gcttcttgcg ttatataaaa gtgtaaaaaa ataaaaggag tgttaaaaat ggcaaaaaaa
    17761 acaatcatgt tagtttgttc agcagggatg agtacgagtt tattggtcac aaaaatgcaa
    17821 aaagcagcag aagaaaaagg gatggaagcc gatatttttg cggtctctgc ctcagatgca
    17881 gataataatt tagaatctaa gaatgtcgat gtattattat taggtcctca agtacgtttc
    17941 atgaaagctc aatttgaaca aaaacttgcg cctaaaggta ttccattaga tgtcatcaat
    18001 atgcaagact atggcatgat gaacggagaa aaagttcttg cacaagcaga aaatctaatg
    18061 aaataaatca agtatgcagt tgggggtata acaacgtgga agatcaacaa aatctagaag
    18121 ccatcatggg gttaatcatg tttggcggta atgcaaagag cgatgcaatg gaagcgattg
    18181 cggctgctaa aatcggtgat tttgagttag cggataggaa aatcgctgat gcagaagaat
    18241 cacttataca agcgcatcat gcacaaacag acatgttgac acaagaagca caaggaaatc
    18301 atatgcaagt gacattattg actgttcaca gtcaagatca cttaatgaca tctattgcgt
    18361 ttactgattt agcaaaagaa atcatcgaac tttatcgtcg cgtatacagc gaataaatac
    18421 aacttctttt ttgaacggaa gaagaaaaat ttttgaacgg attttttatt ttgttataag
    18481 gagaactctc tttatataaa aaacttatag gaggaagtaa aaaatgaacg gactatcaag
    18541 ttggatggag aaatacatcc tgccggttgc cgcaaaaatc ggagcgcaaa aacatttagt
    18601 tgctttgcgt gatgctttta tcggcacact gcccgcaaca atggcgggat cagtcgctgt
    18661 aatgatcaat gcaattattc gtgatttacc gcaacagttt aacagtacct atgctgacag
    18721 tttagcagca gacaaaggaa tctttgccgt attgaatgtc atcatcggaa tcaatggttt
    18781 tgtctggaac ggtacactag caatcgccgg attgatcttt gccttttctt gggggtataa
    18841 tcttgcccga gcttatggag taaatgatct agctgggggg atcgttggtt tagctacctt
    18901 gattcaaggt atttcctttg gtccagcatt atcagcagca ttagatgtga atgttccttc
    18961 aaatgtcgca gatgcaatca atagttctga gattggtgca acgattgcag atggcacact
    19021 gtcagttgga ttatggggtt ggctaaaact agatcatttg aatggaaacg cttattttac
    19081 agttatgatc atgggagcta tttcagtcat tattttttgt aaattgatgc ttgccaacat
    19141 tacgatcaaa atgcctgatt cagtaccacc agcagtatct aaagcatttg cagccatttt
    19201 accagcgacg atcgctttat acgtcattgc tattatcaat tatatcgtag gtcgtgtagc
    19261 tgatgggcaa ctgatcattg atttggtgca aaaatatatt gcagaaccat tcctaggatt
    19321 atctcaaggg ttgggagcag tattgatcgt cacagtattt gttcaagtat tctggttctt
    19381 tggtattcat ggaccaaatg tattggcacc agtgctagag ggaatctggg gacaagcaca
    19441 actaatcaat attgatattt tccaaaaagg ttataacgga aaaactggta caccagccgt
    19501 gttagatgca atcgaagatg gaaaagctta tatgtgggtc cgcggatcgt tcgatgcttt
    19561 tgcatggttt ggcggatcag gcggaacgat cgtcttactg atagctatct tgattttctc
    19621 taaacgtgca gattatctaa cagtagctaa attatccatt gggcctggta tcttcaatat
    19681 caacgaacca gtgatgtttg gtctgccaat cgtattgaat gcaatcatgt ttatcccatt
    19741 cttgattgct ccagtagtag caacagcaat tggatggaca gctacttatc ttggcctagt
    19801 cgctccggta tcgcagcaag tcacatgggt agtaccgccg ttcttattgt cattcttagc
    19861 aacaggagcg gattggcggg caccgatcgt aacattagta tgtatggtcg tgactttctt
    19921 gatttgggca ccatttgtca tctctgcaaa caaaatggac cctgccgcag gcgaacaaca
    19981 ataagatgaa cgtgaggagc gggacgcttt aagtttcgtt cctcataatg aattttctag
    20041 gaggaactga aatgattaca attgccagtg caatgcaaca agatgaaatc aaagaattgt
    20101 tagaaggata caatgaagaa ggaatgactt ttacatttaa agaaaaacaa ggaatcaagc
    20161 tgttctttga aacaagtgca gaagatccgg aagttgcggc taaaaaagca aaggaactaa
    20221 tcaaagcaca gccttgggga acagtccttt attttcaatc aacagctgtt tagaaaatgg
    20281 tttaaaaaga taggatggtt gagatgataa aaaacggttt atttttatgt agtattggtt
    20341 ctttgatcgt gttgtatagt ttgaatccac attcgcaaag gtatgatttg tatagtatgg
    20401 tcacaggtct ttttctaaca ggattaggaa tctatttctt catgaagggg aaaaaaagag
    20461 aagaagcaaa ggagaaaaat aactaatgga aatcacgaaa aaattaaata ttccagcatc
    20521 gtttttttac gataaagtta tggactctgt ccttttcgat gtacgtaaag cgaccggcaa
    20581 atcagtaact agaaaacagc tgaaaaattt ggagtacgtc aaacaattct cgcaaaataa
    20641 ccgggctcgc ataaaaatcg aagaagtcat tgaaaaccag tcttataagt ttcgtacatc
    20701 gacaacgaag aatgaattcg tcgttcatta tcaaatcaaa ccattggacg attcgacctg
    20761 tgaggttcat tacacagaaa aaatggagtc ttatggcatg cttcaaaagt tgaatgatat
    20821 gcttttagga acaatcttga tgtattttaa acggagacgt ttcaaaaaaa tgctagatat
    20881 gatcgaagca acgtattaaa tcaatgaaac atggtactat ttagttattg aaaaatgaag
    20941 taagctagaa ggaatttttc ataggaagat agatctttcc tgcgaatcct cactagcttt
    21001 tttgtaggaa ggggcagcaa aatgacaaac aaagtaagag agctaggtgt atcttcgatt
    21061 gaggaaatgt ttgatttagc cacttatgca tttaatgggg cagatacacc tgaaaggcga
    21121 gaacgttttc gtacacttgc ggagaattct tggaattacg gattttttga tgaggaggat
    21181 cggctaacaa gtcaagtcat ggcgacccct tttccagtga atttttacgg acaagagtac
    21241 ctaatggccg gcattggcta tgttgcttct tatccagaag caaggggaca aggcgggatc
    21301 aatcagatca tggaaaagat ccttgaagat tgccgtgatc gtaaagtaag cttatcttat
    21361 ctggcgccat tctcctatcc cttctatcgg cgctatggct atgaacaatc atttgataaa
    21421 atcagttatc aactaaacag tcgtgatgtg ccgtttatca aaaaaaaatc tggaaaaata
    21481 aagcgtatgg attttgaaga tgcaaaagct gcaatcagaa gaatttatga acagatgccg
    21541 gaaaaccaac gagctggtct gaagcgcgaa gactggtggt atacgtataa attcaagcaa
    21601 aaaggcaatt accagtttgc tttgcatttt gatgataatg aggaagcaac gggatatatc
    21661 gtttatcagc ttgcaacgtc ttcctttatt attgcggagt ggggattttt ggatcatgat
    21721 gctttttgca gccttgttcg cttcgtaagt tctcacaatg gtgcttttga aacatttgaa
    21781 tattcatgcg gctacggcgg taatgatcaa aattatctat tagaaaatcc atttgcttct
    21841 gtaatgatca caccttatat gatggcgagg atcgtagata tttctttgtt tttggaaaat
    21901 tatccattta aaaagagaca agctgcattt gccctacaaa tagaagaaga tcattatgcg
    21961 aaatggaatg aaggaatcgt ggaagtaaca atcgaaaatg gaaaaaatca tctctcaaaa
    22021 gtagaacaaa caaaactccc aatcgttcga ggaagtatcc aacatatcac gcagctatta
    22081 ttagggtatc gtaaagtgga agagttggta ttccaagaaa gaatacaggt gacaaaagaa
    22141 tatgaagaaa cgttaagctc aatcttgcca cagcaacagc caattcttaa tgactatttt
    22201 tgataaaaaa cgggtgatga caatattttt gtcaccaccc ttttgttttt catttggcat
    22261 ttgaaggaaa atgttaaagt aaatcctctt ttttcgtaaa tttgaatttt ttgttttctt
    22321 ttgttttagg gttttctttt ttgtggtctt ggattgcttc tttcgtaatc ttttcccgat
    22381 tttttttaga tgaatctaag cgttctgcgt atactttttc ttttggctta tcattcattg
    22441 gaaaggacct ccctacatta tttcattcta cctctagttt atcatctgtt aaggactagt
    22501 ggaaacgttt tgcctaactt ataagctgat ttttttagca gataagcttg caagtaaaaa
    22561 acaaacaagc tacaattgat ttattgaaat tgttcctgta gctcagtagg atagagcaat
    22621 cgcctcctaa gcgataggtc gctggttcaa ttccagtcag gaacgtataa ataacccctg
    22681 tatcctttgc ggtacagggg tttgttttgt atttgtgcca aaaatgtgcc aaactagtta
    22741 tcatttttat ataaactatc gaacacagta ttgatttttt ctttatcctg atcttcaagc
    22801 tctcgaacaa tatgaagata agtagacatt gtagtttcta aactgctatg tcctaaacgt
    22861 ttcgatacgc taagtatatt tactccttga taaagtaaaa tagatgcatg cgtatgtctt
    22921 agtccatcaa taaacagtcg aagagaaaat agggaaaaat tgattactaa agaaaaactt
    22981 actaggtcga cggtgtagtt gattttcata cttcaataga ttcgaattaa acgatttcga
    23041 ttggcttata ccagtcctat ccaacgaaag atatatcgtt cttaactcta gcggtataac
    23101 ttgaagcttt acgtaaagcc aatgggaatg aactggagat gactttttat tatttttagt
    23161 aatagctgat gggttaatac ttacaacttt ccttttcaaa gtaccatata aaaaaa
//
LOCUS       DAFFSV010000036        22857 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFFSV010000036 DAFFSV010000000
VERSION     DAFFSV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.036"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(104..1336)
                     /locus_tag="LR110_001256"
     CDS             complement(104..1336)
                     /locus_tag="LR110_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBL4822227.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="LR110_001257"
     CDS             1591..2160
                     /locus_tag="LR110_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4822228.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="LR110_001258"
     CDS             complement(2338..2778)
                     /locus_tag="LR110_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBL4822229.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="LR110_001259"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="LR110_001259"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBL4822230.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="LR110_001260"
     CDS             3732..4655
                     /locus_tag="LR110_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBL4822231.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="LR110_001261"
     CDS             4731..5870
                     /locus_tag="LR110_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBL4822232.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="LR110_001262"
     CDS             5863..6663
                     /locus_tag="LR110_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL4822233.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="LR110_001263"
     CDS             6663..7490
                     /locus_tag="LR110_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL4822234.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="LR110_001264"
     CDS             7468..8202
                     /locus_tag="LR110_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL4822235.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="LR110_001265"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="LR110_001265"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBL4822236.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="LR110_001266"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="LR110_001266"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBL4822237.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="LR110_001267"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="LR110_001267"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBL4822238.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="LR110_001268"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="LR110_001268"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBL4822239.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="LR110_001269"
     CDS             11787..13820
                     /locus_tag="LR110_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822240.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="LR110_001270"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="LR110_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="LR110_001271"
     CDS             15353..16159
                     /locus_tag="LR110_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822241.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="LR110_001272"
     CDS             16171..17391
                     /locus_tag="LR110_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822242.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="LR110_001273"
     CDS             17381..18967
                     /locus_tag="LR110_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL4822243.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="LR110_001274"
     CDS             19006..21144
                     /locus_tag="LR110_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL4822244.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21512..22495)
                     /locus_tag="LR110_001275"
     CDS             complement(21512..22495)
                     /locus_tag="LR110_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBL4822245.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7177 a         3899 c         4711 g         7070 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttaag aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    21301 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    21361 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    21421 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    21481 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    21541 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    21601 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    21661 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    21721 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    21781 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    21841 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    21901 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    21961 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22021 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    22081 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    22141 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    22201 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    22261 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    22321 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    22381 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    22441 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    22501 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    22561 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    22621 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    22681 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    22741 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    22801 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtg
//
LOCUS       DAFFSV010000037        22327 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFFSV010000037 DAFFSV010000000
VERSION     DAFFSV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.037"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            16..255
                     /locus_tag="LR110_001276"
     CDS             16..255
                     /locus_tag="LR110_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822246.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            341..1012
                     /locus_tag="LR110_001277"
     CDS             341..1012
                     /locus_tag="LR110_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL4822247.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1065..3041
                     /locus_tag="LR110_001278"
     CDS             1065..3041
                     /locus_tag="LR110_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL4822248.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAVKNAEGKYYGGLVDTDKDGVKEAVWVDNVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3054..3809
                     /locus_tag="LR110_001279"
     CDS             3054..3809
                     /locus_tag="LR110_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL4822249.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            3806..4630
                     /locus_tag="LR110_001280"
     CDS             3806..4630
                     /locus_tag="LR110_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822250.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQPVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4932..7031
                     /locus_tag="LR110_001281"
     CDS             4932..7031
                     /locus_tag="LR110_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL4822251.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVLDGKNYDGALWYVEANGTWKKADTGGKLTVSSNTVTYTASDSFLANA
                     ANYGRDYILQLHMKAKNDAKGIAPNVATVTFNNDPNKGTQRTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQDPVEA
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7069..9126)
                     /locus_tag="LR110_001282"
     CDS             complement(7069..9126)
                     /locus_tag="LR110_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBL4822252.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNHEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9240..9452)
                     /locus_tag="LR110_001283"
     CDS             complement(9240..9452)
                     /locus_tag="LR110_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822253.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9639..10106
                     /locus_tag="LR110_001284"
     CDS             9639..10106
                     /locus_tag="LR110_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822254.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10103..10345
                     /locus_tag="LR110_001285"
     CDS             10103..10345
                     /locus_tag="LR110_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBL4822255.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10363..10665
                     /locus_tag="LR110_001286"
     CDS             10363..10665
                     /locus_tag="LR110_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822256.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10736..12790
                     /locus_tag="LR110_001287"
     CDS             10736..12790
                     /locus_tag="LR110_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822257.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     TNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            12790..15402
                     /locus_tag="LR110_001288"
     CDS             12790..15402
                     /locus_tag="LR110_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBL4822258.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNM
                     HDQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVI
                     RKRLLLEAEKAVKE"
     gene            15399..17930
                     /locus_tag="LR110_001289"
     CDS             15399..17930
                     /locus_tag="LR110_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822259.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            17980..18312
                     /locus_tag="LR110_001290"
     CDS             17980..18312
                     /locus_tag="LR110_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822260.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18313..18906
                     /locus_tag="LR110_001291"
     CDS             18313..18906
                     /locus_tag="LR110_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822261.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19069..21093
                     /locus_tag="LR110_001292"
     CDS             19069..21093
                     /locus_tag="LR110_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBL4822262.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            21093..22298
                     /locus_tag="LR110_001293"
     CDS             21093..22298
                     /locus_tag="LR110_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL4822263.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
BASE COUNT         7943 a         3512 c         4523 g         6349 t
ORIGIN      
        1 aaggtaggaa aactcatgaa ttatgaagaa atcgaaaaca gaaaaaaagt gtcgaaagaa
       61 atggaagaaa agttgctaaa aacgatgaaa caaaaacacc ttaaaagatt atctgtcatg
      121 caatatatca atgatatgca gataacagga aaagagaaag cttgtttgtt aggttcaatg
      181 aaaaactttg aacagttgag acgaacttat gtgaaaaaaa gttctaattg tcaactattg
      241 ttagaagtct catgacattc attaatagaa ttcattttta aaactttttc tatggaaatg
      301 agaaaaagat tttttgtttt gtctgaaagg aggtagctgg atgagaaaat ggttatttgg
      361 ttttttagga gtcgctttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      421 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      481 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      541 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      601 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaaggtg caaatcaata
      661 cactttgagt cttggagcgg ctacttattt ttatgaagat gctgaaatgg gcaaaggaaa
      721 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atatccaaaa
      781 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
      841 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
      901 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
      961 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1021 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1081 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1141 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1201 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1261 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1321 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1381 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1441 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1501 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1561 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1621 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1681 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1741 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     1801 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     1861 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     1921 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     1981 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2041 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2101 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2161 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2221 ttaaatgggg agatacagca ggacaaggac tttcttatgt cgctgctaaa gcaaatatca
     2281 aagtaacaaa taaagatgga tctgtggtat taaaagaaaa tacagattat aaaatccaaa
     2341 attcagaaaa tggctttgtg atcgatttta tcgtgaataa tggccaagta agtgatactg
     2401 tagcaagttt acacggacaa gatcttcaaa tgacttataa catgtatgta aatgacagcg
     2461 cagctgttgc caatccatta acaaatagcg tggatttcgt ttataacaac aatccattta
     2521 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggt gcaaaattct
     2581 taaaagtcga tagcggacta tttggaacag gaatcaaagc aacaccgctt gaaggcgcag
     2641 aatttgctgt taaaaacgca gaaggaaaat attatggtgg attagttgat acggataaag
     2701 acggtgtcaa agaagcagta tgggtagaca atgtagcaaa tgcagcgatt ctaaaatcag
     2761 ataaagaagg gcactttgaa atcactggtt taactgaagg tgaatattca ttggaagaaa
     2821 caaaagcgcc tgaaaactat caaaaattaa cagaagatat tgcatttaaa gtaaacaaaa
     2881 actcatttaa agaggaaaat cgaatcactg ttaaaaataa tcaaaaagca gctattccat
     2941 taacaggtag taatggtttc caaacgtatg tattgatctc ttgcttgttg ctaggagcag
     3001 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3061 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3121 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3181 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3241 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3301 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3361 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3421 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3481 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3541 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3601 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3661 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3721 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     3781 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     3841 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     3901 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     3961 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4021 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4081 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4141 agtagcccag caagaaggta tcaatggcgc aaagtttatt gtctacgata tcacgaatat
     4201 cttagaagaa atgtcacaag atgataatga gaaaaatcaa cctgtagaaa taattgagtc
     4261 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4321 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4381 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4441 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4501 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4561 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4621 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4681 agccgcaaca ttattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4741 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     4801 taaaaagctc ttgatcagtc caatttttat ggttagtttg gttcttctta caggattggt
     4861 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aaatattaga
     4921 tggagggcat tatggaaaat aaaattcatt gcactacggg aagagtaatt actttagtgt
     4981 tgtttttgtt ccttcttctt tttgcgaggg gaactgcagt ccaggcctta gaaaaagttg
     5041 atccgagtca aatcaaagaa aataataatc tggtgcttta tcatgatagt gattcattga
     5101 gaaatatcga agtaactggg atcaatggat ttaagatact agatgagtat tcaactaaaa
     5161 aacaaaaagt acttgcttgg ccaaaagtca aagatttgaa aagtacgtca aaaatcgttg
     5221 cccattatca aaaagtaggt acttatctag gaaaagaaat tgattgtgac gtgacttttt
     5281 ctaattttgt agcgcacgct ggggttaacc aagacggatt agctaaaaca attaatggca
     5341 aaaagtatta taattatttg ctctttgata aaaatattta tcgaggattt ttcatgcagt
     5401 caatcaatgt catgaatgca aaactagaat ttaagtatgc aggaacaaaa acaaaagtag
     5461 ctttgagtga accacagcca gaaattcaat caaaaatgag atcgatgaga gcagctggta
     5521 ttgatttaaa cgatacattt ctatccattt atagtttaaa tggacagtat cagcaacgtg
     5581 tgtcttggta taatgacaat aatgaatctg tcggtgaacg taatattgat atgagagaat
     5641 ttgttgggta tgaaaaaatg ggtagcttac cttattttgt cacaacagat acagcatgtg
     5701 cagaatacaa agctcctgcg ttatcaacaa acaatttaac ttcaaaagta gtgggaggac
     5761 gtgcagaaaa ggcttatagc tcgaatgatc atttcaccga tgttgtagga gctgatactt
     5821 atcacagaag tggtgtaacg tatacgcttc aaggcgcttc cccaacattc atgattggcg
     5881 caaatacgaa tagtatgatg tttagctttg atactgcatt gctatggaca ccacaaccat
     5941 cgaagcctac aaaagaagtg tttaacaaag ctaatactga agaggcagca cacaatattg
     6001 acaaaaaagt gattccacaa ggatcagatg tttactatca tattcaccaa aaatttgatg
     6061 cattaacagt caacacaatg aacaaataca aatcatttaa aatcactgat acctttgaca
     6121 gcaaaaattt tgatatggta ttggatggga aaaactatga tggcgcattg tggtatgttg
     6181 aagcgaatgg tacttggaaa aaagctgata caggaggaaa attaactgta tcaagcaata
     6241 cagtcactta tactgcttct gacagctttc tagctaacgc tgcaaattac ggtagagatt
     6301 atattttgca gctccatatg aaagcaaaaa atgatgcaaa aggaatcgca cccaatgtgg
     6361 ctactgtaac gtttaacaat gatccgaata aagggacaca aagaacaaat acagtagaaa
     6421 attatttctc tcttaaacca aataaaaaag tggaacaaga tgggaaagat gttgatggtc
     6481 gaaatgatgg taaaaaagga acgcctacag ctcctttaaa tgcaggatct gaagtacaat
     6541 atctagtcac acaaaaatgg catacaaaag gtgtagacat tgcgctcgat cattacaagc
     6601 aatttagtat tcaagatcca gtggaagctc gtttgacgta taaagaaggt tctgcgcgag
     6661 tgatagatat gagtacagga aaagatatta cttcagaagg tacactaacc tatgatagca
     6721 attcaagaac gctgaagtgg gaagcttccg ctgatttctt aagtaaaaat cttttagatg
     6781 gacgagaaat tcaactgata tttacagcta aaactccttt gaatgaagaa ttggatatcg
     6841 acaaccaagc aaaagtttca gtagaaagta ttgagaaagc aactaatgta gtaacgattg
     6901 gggtaaaacc taacttacct cagatcattg tgccaaaaac tggatcgaat catttagtgc
     6961 taactgttat tggaagtata ttatttctta ctttagcatc attgggctat gtagttttaa
     7021 aactcaaata aatataaaaa caaggctgag aatcagcctt gttttctttt atttgaagat
     7081 tggattcgca ctaataaata cattatctac gccaattcta ccagcagagt caactacaaa
     7141 attatgacct aatccgagaa tactttggtt attataaagt aaatttgcat tatgggcagg
     7201 gctatttttc catcctacca tggcatcgat agcaccatcg tctgataaaa ggtcatcaat
     7261 atacagccca gtttgactta tgttttctcc cttagcaccg tagcagccta agctttccaa
     7321 taaatcttgg aaagtaccac caccagggcg atagtgactg aagctgactt gtaattcttt
     7381 agctcgaata tctgcagcct gttgaacaat atcattgaat tgcattgggg caacaccatt
     7441 ttgttggcga taatcattta accaagcaag ttctttttga cgcataaagt tacgtaattc
     7501 gtctaatgaa atctttttat atttgaatgt gattgtttta ttttgatcga cgttattaat
     7561 ggatcgttgg ccgtttttat ccacggtaac ggtaggatcg tcatttctgc acaagaaata
     7621 attttgatca gtcatgtttt tccaaggagt agcaaagcta tccccttcgt atactgtttt
     7681 tgattcacta gagagaacag cgttggtatc agcatttaca tgattaacag tcacattaaa
     7741 tttacgagtg tagttgaaaa tataattttg actttcagtc acattgttta aggttactga
     7801 atttgttggt tctacccgat cgtctaaaac atatccttga atagaaggag cagttaaagt
     7861 gacactgtca ccgtgagtga cattgactat ctgttctttc aaaatattaa ctcgtccttc
     7921 gtttttgtag ttgttattgc acaacaaatt gatttgatgc acttgattat aattgaatgt
     7981 gattgttgta ttacctgtga ttgttttgct ttgagtatca ttaccaatca ctttgtagct
     8041 aaaattatct ttagcgcctt cgctatattt aaatgctttt gccttagcag tgtaagtaga
     8101 accagaagtt acttttgctt gatctgtagc aatcacttga cctgtttgat tgtcaatatg
     8161 gttaatagta accgtatatt cttttggtgt ttcaggtttg tgttgcaacc ctttaaatgc
     8221 agcatcaaga ctatttttag cattatttac ttgggcttga gaagccttag aatcgtctag
     8281 tactttttta gcattggata aagcagtctg aaaattattc caagattctt ctgtaaaatc
     8341 tcctttagca gttgatttta ctcggttgta taaagcttgt aattcagctt tattcacagt
     8401 aggaactggt tttttcttta atcctttgta agcagaatct aatttatttt tggcactatc
     8461 cacttgtttt tgagtcgctt ttgagtcatt taacactttc ttagcattgt ttaaagcggt
     8521 ttggaatgcg ttccaggtat tgtcggtata gtcacctttt ttagttccct taacgctcgt
     8581 ataaagagct tctaaagctg atttattcac tttgggttta gaggtgtcag cgccatacca
     8641 accgatacct tcgtatttcc agccatagtc tttggttaac cattgtactt ctttccaact
     8701 tgcagtgtag tgatgtgagc cagattttac accaggaagg aaaaggcggt agatcggttt
     8761 attatttttt tcggcagaac gccaggctgg cccttcataa gtccagccgt aatttttagt
     8821 caaccatttc acttcatccc aactagctgt gtaatggtga tcacgaagac ctttattgta
     8881 caaacgatag acaagatggc caccgctagt cggtgcttcc caggcggggc cttcataatt
     8941 tccccatttt atctttacta gatggtcacg ttcttttttg ctagaggtgt aaaaatgttc
     9001 atgattattt ggattgtaaa ggcgatacat gacgttagtt gaagcagcgt caacagatcc
     9061 agcaagacta agtgaggcag caaatgttcc aatagctaaa acagtttttg gattaaataa
     9121 tttcattttt tcaactccta aatttaatta aaaatgttta catgattatt atatatcggc
     9181 taaccgatat agtcaataaa aaataagaaa agccttatat aattttggct tttcttattt
     9241 tatttgttag ttgaagtatt aatatcaacg atttcttcag ccaatttatt taatttcata
     9301 gcatttatga atctcatgtt gtctatacta gagcgtttgt tggcaaggtc acttactgta
     9361 gttaagggta cgccagattc tatggatata gaacgaactg atttaccact atcaaacagc
     9421 aaccattcga ttttcttttt atcagcgtac atttcaatgc tccttcttgt tttttcttta
     9481 ttatagctct tttttgtaat aaatcacaat atcaagagaa aaattttcta tagaaaggtg
     9541 gatttttatg agtattcgtt ctccgtgtat ccaaatgagg tttgttgcta ctttcgttat
     9601 ttgatatact aaaaataaac aacgaaagaa ggaataaaat gaagaaaaag caactatggt
     9661 ttgtctctat gtttgcaata ttttttatta ttttaagtgg atgttcaagt aataagaaat
     9721 cggaagctaa agaagataat agtttattgc aaccaggaac tgtttggaaa gaagaagtag
     9781 gtggtcttat tcataattta aaaattatag atgatactac ctgggaatat tctgaaagtg
     9841 tatggcatcc agatcctgta caaataacag ttaagaggca aaaagactat gaaggtttag
     9901 aaagatacaa aatagtagat tctgcaggta ttagggaatt tataaacaaa cctgattcat
     9961 tatttattgt tgttccctat gagaaggatg gcgtaaaaaa aataattttt cttggatcta
    10021 gcaaggatga gaagcgaacg aaagagaaat taattcatga tggatcagag ttaaataaat
    10081 acaaattaca aaagactagt gagtgattgc tagtcttttt atttatcctg aaggaggaat
    10141 cacagtggca aaagttaaaa caccattcga aaaagccgcg tctaaaatct tagaacgccg
    10201 tggtgtttct gaaatacagt ggaaaaaaga agttcttgaa aaagcacaat ttgacctatt
    10261 acgaggaaaa gataaggact tagaagaata tatctatcaa cgtgaacgtg aaaaacttgt
    10321 attcgatgaa attattaaac attaaaagga gcgaataatc ttatgtttga atcaattaca
    10381 atgaattttt taactcaacc gaaagtagta gctgcagttg atatttttag agcattacaa
    10441 actgccgcaa ctagtttgaa aaataatctg atgatttttg ctgctgttgc ggcagtggct
    10501 tccattgctc ttggaggtat tttcttcttc tttgggcgaa gaggagctga aaacgggaag
    10561 acacacgtag gttatgtatt gttgggggta ggagttattt tagctgccgc ttctattgta
    10621 gtttcaatgt tctcaatgtt tggacaaacc ccacaaggac tataaaaaaa caagtagaag
    10681 gagtaaaaag tacgtggcta ctttttactc ctttttttat ggaaggagaa tattcatgtt
    10741 ttttacgatt tcaggcaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    10801 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    10861 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    10921 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    10981 tggctcactg gattatcaaa tttcagaagg tttttatcct acattgattg atttaatccg
    11041 cctagatata tttaaagata cggatgaaac catgagtgtg gaagagtctt atcgaaacga
    11101 aaaaatttta caagttttag aagaagagtt agaaagcgac caaagaaaag tgaatcaagt
    11161 gagtgaagtc gaagcaaacg aagaagtacc atgggaacgc ccaatctttc caaaggatga
    11221 atcaaaagaa aaagagaaag gaattattga cgaacaagct gacattttga ctcataaatc
    11281 aatatttgag gaagagaacg aggtaatggg tcaattggag gtagatccct ttgaagaaac
    11341 aatggaggat gaagagacgg ccgaagtaga agttgatcca gttgaagcta atgaagaccc
    11401 aacatggact gaaggggatt ctcaggaata tattgaacca cttatcagtg aagaagtaga
    11461 gaaaaatgaa acagagatac aaaaggcaac ggtatttgat tatattttgg atcaatatcc
    11521 gccagagcat gaatggatga aagaaaaaaa taaattattt ttaaatgaac tttattcgaa
    11581 atatcaatta cctcaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11641 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11701 tgcacaagaa gatttattag ctacttttga agaacgtcaa caagaaacag atcaagaaat
    11761 taatgagtat gcgacggaac agtcgtcgaa ttgggagaaa gcaaaagagg aactaaataa
    11821 agaagaacag cgagtgattg aagaagaagt acaaaaaatt aaagagcggt acgaaaagaa
    11881 acgtgacaat gcgtattcct tgtgtgtaga aaagatcaac aattttactc agacgaatca
    11941 agaagcatta tcgaaagaaa aagaaaactt attaaaagaa cgtataaaag aacgaaaaga
    12001 agaattttac cgagggttgc gtactgataa gctaaggatt agcaaagaat taaatgaaca
    12061 actgaataaa ctctatcaaa ctacgactga agttctgatg gaaaaacacg agcagatcca
    12121 acaagaatta caagcacaaa ttccaaaatg gaaaaaagag tatgaaattc accaacaaga
    12181 attgaaagaa caagaagaaa aacaacggaa aaaagaaaaa gagcgcgaac aattagaact
    12241 aaaaaaacgt gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggctaga
    12301 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12361 gcgacaagca cagttaaatt atctgacaca tcctcaacca gtagctgctg ttcaaacgcc
    12421 agtgaacaac caaacaaatc taactccttc accccagcgt tcctttaacg gatggatggt
    12481 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12541 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12601 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12661 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgattcta gtaaggctaa
    12721 agaaggatca agcgattcaa ctgtacaatc aaacacttcc gaaagttcag aatcaaatga
    12781 ggtgaaataa tgcgtgttag agaaacaacg aagcgaaaag acaaacgatt caatttagaa
    12841 aagaataggt atctttctca tttctatcgc tatactccct caggagaaga gctaccagaa
    12901 aaacatgaca aacgcatttc gaaaaagatt ttttatagtg gacttgtata cctcccttta
    12961 gagtatttag taacaagagt accagaagtt ttcaaccgat tggaaattgt taagggaagc
    13021 ttacaaggaa taaatctgtt tcaattacag gaaatgccac aggtctttca ttttgattca
    13081 gctggttttt ggaatccatt tactcctaca actagtttgg gtacacgttt agcaatttcg
    13141 acagtttgtg tggttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtcct
    13201 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13261 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13321 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13381 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13441 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13501 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13561 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13621 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13681 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13741 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    13801 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    13861 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    13921 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    13981 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14041 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14101 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14161 aaagtaggct ataaaggatt ttgtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14221 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14281 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14341 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14401 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14461 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14521 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14581 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14641 acagctggcc gaaacatgtt gtgggatttg tttatccaat cctatcagca actttatgcg
    14701 aagtatgggg aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    14761 atgtcgacag acgataatac catagaagaa ttttccaaaa aagtggggaa taagacggtt
    14821 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    14881 gattcagatc gcatcttaac tcctgaaaga atttctactt ttttagaagg tgaaacgatt
    14941 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15001 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15061 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15121 ttacaaattg attacgatta ttttctacct gatatctatg cacgtcgact ttatcacaat
    15181 atgcacgatc aaatagaaga ggaagctaaa gaaaacattt ttattaaaat cgttcaatct
    15241 ttagacaact caacggcaca aaagttactt attcaatttt atgaagagaa gcaaatagat
    15301 cgtttgcaag aataccttga aaaattgcgt caaaatgaag aaattacaga ggttataagg
    15361 aaacgacttt tgctagaagc tgaaaaggcg gtgaaagaat gatcattgga ggaatatttt
    15421 ccggcattgc cgactggtgg tcaggcttat ttggggatgg ggatgcaaac cccaaaaccg
    15481 taacgaaagt cttacaagat tatggtaatt atctgcagta tcaagatgta tttagttgga
    15541 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15601 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15661 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15721 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    15781 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    15841 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    15901 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    15961 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16021 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16081 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16141 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16201 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt atcttagctg
    16261 gtgaatcttc tttggcggtg gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16321 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16381 gccaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16441 cgattttaga attacgaatc tatacattaa tgctttcagg tatcggtgat ttgcatgcgg
    16501 caggaaagat caatggattc ttgtatgttg ttgctttaat tgtgctaact attgcgctat
    16561 tcaaaggctc tcaatcggtt acgaagattt tcggtgttga tacgagtctg aaaaacggca
    16621 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16681 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16741 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    16801 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    16861 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    16921 cagctgaaga tcttacagaa aaagccaaag acaccttaga agagaaatta gacggcacga
    16981 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17041 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagttaatg
    17101 cagcagtaaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17161 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17221 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17281 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17341 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17401 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17461 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17521 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17581 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17641 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17701 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    17761 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    17821 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aactaaaaac aggaacaatg
    17881 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    17941 agttaggcct tttatttata tgaaaaggag aaatcaatta tggataaata tataaacatg
    18001 aaaaaccctc caagatttag tggagaatgg aagtttttcg cttttttctt tttagatcta
    18061 ttaacgcttg gtggctgctt tttttgggcg aaaatggtca ataatcttgt ccacttacca
    18121 ctcttttttg cactcttgaa ttatggctta tggattgcga tagcgttagt gattgtttgg
    18181 cgaccctata ataacccagg tcaacgacaa atttttgcga ttatcgagtc tatttttttt
    18241 gcggacaaga accattatca tgcgttagat cctaaccgaa attatcatgg tacgtataag
    18301 gaggatgaat aaatgcgagt caaaggacaa aattcagttg aaaggaaatc cattttgcag
    18361 tttggaaaac cagaagagcg ctctatcttg gacttatttc cgtataaatc ctgtacagaa
    18421 gagggagtct taattactag agaagatcat tttcaacgtt attatcgtgt agtttctaca
    18481 gacgtggaag gattaaatga acaagagaaa ctagaacgaa tgaatcagtt gaccaccgtc
    18541 atgcgtacgt atgtgccaac aattaagtta gttagtttga ccacagagac ggatctaacg
    18601 gaacaaatcg tgcaaaagcg gcaattatta gataaaaatc gtatggaaca gtccttagga
    18661 agaaatctac aacgattaaa aaaatatgaa aagaaattag tcgaagaaat ccaggaattg
    18721 aaacgagcag aacaagaacg tcctgactta agcttctttt tcgtcattga aggaaagaca
    18781 atcaaggatt tacaaaccag aaaccgtcag ttaatgcgat ctagtggcat tttagggtta
    18841 aaaccactct acaaaaaaga attgatcaag attctgtatc gaatgaacaa tatgaatgat
    18901 gagtaggaat ggagaagaga gaatgaaata tccgttgata tggttagaaa aagatgttcc
    18961 gaaggaagag accactagaa aatttcctca cactttaagc ctgggtaagt tgggaaaagg
    19021 gcaatctttt aaccaagtaa agaatcaaac aagaagaggt gactgtacat gttagaagca
    19081 attaaaacag ctttttcacc acaaaatgat caaaaagtac ttgatccatt aaagcaacta
    19141 ggaaatcggg aaatagaaat tttaaaatcc aaagggtatg atttagattt tttaaaacaa
    19201 gtgatgccac aaggtgggtt aaattttgat gaagaatatg tgacgtatgg taatggttat
    19261 tcacgatgtg tgacagttta tcactacaca aaaaatccta cgcttttttg gttggccaat
    19321 ctcatgaaca atcctcatac agtggccaca ctcgatgtta gcacagatga aaagcaaaaa
    19381 gtgattgatc aaatcaatcg tgccttagat gaattaaatg atcgatcaga taatgaacga
    19441 aaagcgactg accgaaataa aagcgcagaa gaatacctgg aactagcgga atatgctcgt
    19501 aaattgactt ctaatgggga aatttctaag caagtcaaag tgagagtaca tgtttacgga
    19561 aacacgattc aagaagtaga tgaacgagcg caggaactga tcgaaaatct aaaagggatg
    19621 gattatcgtg cggtgactta tctgcataaa accaaacgag attggcaatc gatgtttacc
    19681 atgttcgatg aacaagctga atggttcgga aatatcaaag ggcaatcact accatcgtta
    19741 aacattggcg gaggtttccc gttccaccac caaacgctaa aagatccagg agggacacaa
    19801 tatggggaaa ccatgacagg cggcccattt gtctggaatg cgacgtataa aaatagtgta
    19861 cggttgtcta ctaatacctt gcttctagga atgatgggag ctggtaaatc gaccgttctt
    19921 aaaatgatcg cggaagccca tttagctgca ggagacttta tttggggctt tgaaaaggga
    19981 aaagacttta ttcctttctt aaaggaatat aacggtatta tggtgcgttt agacggatct
    20041 gacggaatga tcaatccttt agagatattt gccacacgga cgtatgatga agcatccagt
    20101 ttatatgacg atggatcagt caaagatttg aagatcaatg aagcagcgtc ctatcaaaca
    20161 cacttagaca aagtggtcta tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg
    20221 aaagcagaat ttaagaccta cttaaatcgt ttttatgaag agtatggaac agtaccacgc
    20281 ggctttactt cctcaaattc gcgttcaaat acggagacgc aagtcacagg taaagatcca
    20341 gaagcttatc cgacgttcaa agaattttta gactttttag gacaactgga attaccaggt
    20401 gcttcccaag agaaaaagaa tcgaaaagaa gaaatggaat ccatcgtaga atccttatgt
    20461 gagacgtatg gcatgatttt tgatgggcat agtacgattc gacaccttga ccaacaacag
    20521 ttggtctttt ttgacattga ctctattaca ggcttagatc gaggggtaca acaatgtcaa
    20581 atgtacatgg ctatgacgtt gatttggaat caggcattga ttcaaggaag aagacaacga
    20641 cgtttaatta aagaaaaaca aatggaagaa caagacttca aacgcttctt ggcgatgatt
    20701 gatgagtgcc ataatgcgat taatccagat tttattgaaa cggtaaatta tgtcacaaac
    20761 tttcaacgag aaatgcgtaa agtagaagca atgactgttt tagctacaca atccccacaa
    20821 gaaatggtgc cagaaaatat gtcttctgat aacttctcga aactgaagaa agtctttgaa
    20881 ttttcttcta cgaagatctt tatgcgtatg gatgaatcta ttttacgtca tatagaagga
    20941 ttagtgggta aatcaatgac gaactcagaa ttagaatcca ttcctcaaca aaatcaagga
    21001 gacgctgtga tcaattttgg ctcaaaagaa accattcgtg ttcattttag tccgaacaaa
    21061 cgtcaattaa aattgtttga tggaggaaaa taatggaaaa ccaaaatgaa agtttaatca
    21121 agcagtatgt taagcgtcgt gcaaagcggc gcttattttt gtggttgttt ggtacttctg
    21181 caggtttgat tactattttg atcactgtgt tcgtgaccct atttctaata cttgcggcgg
    21241 gctcgattga taattcggat tcagattcaa gcagtggtgg cgaagctttt acaggagaat
    21301 attcggaagg cttaccgatt tataaagaaa tcaaaggaag aggccctttt tctgatgaga
    21361 ttgcgcaata tgccgtaggt gcagcagtga aatataaatt attaccaagt gtgatccttt
    21421 cgcaatacgg ttacgaatca gcgtttggta catcggcgtc cgcaagaaat gatctcaact
    21481 attttgggat tacttggttt gatggttgtc tgtttcctaa aggaactgca cgaggaattg
    21541 gtggcattga aggtggctgg tacatgaagt ttcccaactc aaaagctgca ttttcctatt
    21601 acggctttat ggtagccaca caatcaaatt ttaatgcttg tgtaggaaat aaaagccctg
    21661 gcgcaagctt attgatctta ggacgaggcg gttatgccgc tgcaggaatt actgaagata
    21721 gtccgtatta tacaggttgt atgtcaatta tcacttctaa taaattaacc gaatatgatg
    21781 aattcgccat caaacattgg ggtgaaggtg gcaataacaa tggcacaatt actggtgaat
    21841 ggacgaatcc attccctggc agcagtttag ataaaagttc cttcagcggt ggtcaattat
    21901 ttggaacaaa tccaggcggt gaattccgtc cgaacggctt ccatgatgga ttagattttg
    21961 gttcagttga tcatccagga agtgagattc atgcggttca tggcggaaaa gtcgtgtacg
    22021 ttggcaatcc tggtatttca ggtttgggcg cgtgtgtgat cgtgattaat tacgatggct
    22081 tgaatatggt gtatcaagaa tttgcaaata gtacaggaaa ttctcgagtg aaagtaggcg
    22141 atcaagtgaa agttggtcaa gtaattggga tacgagatac agcacacttg catttaggat
    22201 ttactcgaat ggattggcgt caagcacaag gacatgcatt cattgatgat ggtacatgga
    22261 tcgatccgtt accatttttg aatagcagta aaaaatagat caggagaaaa aatatggaaa
    22321 ataaaac
//
LOCUS       DAFFSV010000038        21906 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFFSV010000038 DAFFSV010000000
VERSION     DAFFSV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.038"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="LR110_001294"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="LR110_001294"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBL4822264.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="LR110_001295"
     CDS             complement(1174..1869)
                     /locus_tag="LR110_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBL4822265.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="LR110_001296"
     CDS             complement(1882..2598)
                     /locus_tag="LR110_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBL4822266.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="LR110_001297"
     CDS             2928..3536
                     /locus_tag="LR110_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBL4822267.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="LR110_001298"
     CDS             3861..4184
                     /locus_tag="LR110_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL4822268.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="LR110_001299"
     CDS             4366..5736
                     /locus_tag="LR110_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBL4822269.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="LR110_001300"
     CDS             complement(5866..7167)
                     /locus_tag="LR110_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL4822270.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9479)
                     /locus_tag="LR110_001301"
     CDS             complement(7521..9479)
                     /locus_tag="LR110_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBL4822271.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="LR110_001302"
     CDS             complement(9656..9922)
                     /locus_tag="LR110_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL4822272.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="LR110_001303"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="LR110_001303"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBL4822273.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="LR110_001304"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="LR110_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBL4822274.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /locus_tag="LR110_001305"
     CDS             13266..13829
                     /locus_tag="LR110_001305"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL4822275.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="LR110_001306"
     CDS             complement(14082..14603)
                     /locus_tag="LR110_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBL4822276.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="LR110_001307"
     CDS             complement(14606..15448)
                     /locus_tag="LR110_001307"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBL4822277.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="LR110_001308"
     CDS             complement(15441..16166)
                     /locus_tag="LR110_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBL4822278.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="LR110_001309"
     CDS             16342..16638
                     /locus_tag="LR110_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822279.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17206..18798
                     /locus_tag="LR110_001310"
     CDS             17206..18798
                     /locus_tag="LR110_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL4822280.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="LR110_001311"
     CDS             18811..19551
                     /locus_tag="LR110_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL4822281.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19719..21068
                     /locus_tag="LR110_001312"
     CDS             19719..21068
                     /locus_tag="LR110_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4822282.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21196..21786)
                     /locus_tag="LR110_001313"
     CDS             complement(21196..21786)
                     /locus_tag="LR110_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL4822283.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6293 a         4458 c         4156 g         6999 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagtcatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaaata aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtatctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caatcaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacaaactt ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcca tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgaacaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaacagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatta ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaaaattt tggaatctta gttgtgtttt tcagaaaact cttttaatat
    19681 ggaagtaatt aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc
    19741 agcgtggact gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg
    19801 ggttgttttt aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct
    19861 atcttattac tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat
    19921 caaacaccaa taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg
    19981 ctttcatcac tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa
    20041 cagccatcgg aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac
    20101 tgattgcatt aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt
    20161 tagagttttg gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag
    20221 cttatatgat cctcacccac tatacgacag atattggcac agccagtatc acgaatctat
    20281 ggtctcatgg cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg
    20341 taacctttgc ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc
    20401 ctgaaaaagt gttgccggaa gctatcaata atattcctat caggatcatc ttattttact
    20461 taggatctct ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca
    20521 gtccatttgt agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt
    20581 tagtcgtatt aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta
    20641 tgcttcgttc tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac
    20701 atcatgtgcc agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct
    20761 tgaattacgt gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt
    20821 tcttgtttat ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg
    20881 gcaaaaaagc tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc
    20941 tttacatggc ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat
    21001 tactaacacc tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta
    21061 agaagtaaga aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac
    21121 gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tcttggagtt aaaagcttct
    21181 gtcccagcct cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt
    21241 ataggaaaaa atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac
    21301 aaaaaagaca ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc
    21361 ctttgacaaa actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat
    21421 caagatacca aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca
    21481 aaaagcttca gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa
    21541 gagcaaaaag atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat
    21601 acgatgatgg ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa
    21661 tgtatttcgg atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa
    21721 tacaagaact ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt
    21781 tgtcacgatg attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa
    21841 ataaccgtcg ttttatatag gaaattttct gaaaattgtt gcatattttt cgaaattttg
    21901 ttactt
//
LOCUS       DAFFSV010000039        20998 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFFSV010000039 DAFFSV010000000
VERSION     DAFFSV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.039"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            591..1280
                     /locus_tag="LR110_001314"
     CDS             591..1280
                     /locus_tag="LR110_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4822284.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="LR110_001315"
     CDS             1267..2475
                     /locus_tag="LR110_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL4822285.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="LR110_001316"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="LR110_001316"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBL4822286.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="LR110_001317"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="LR110_001317"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBL4822287.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="LR110_001318"
     CDS             complement(6578..7273)
                     /locus_tag="LR110_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBL4822288.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="LR110_001319"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="LR110_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBL4822289.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="LR110_001320"
     CDS             complement(8472..8663)
                     /locus_tag="LR110_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBL4822290.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="LR110_001321"
     CDS             complement(8684..9574)
                     /locus_tag="LR110_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBL4822291.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="LR110_001322"
     CDS             complement(9561..10328)
                     /locus_tag="LR110_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL4822292.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="LR110_001323"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="LR110_001323"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBL4822293.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="LR110_001324"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="LR110_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBL4822294.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="LR110_001325"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="LR110_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBL4822295.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="LR110_001326"
     CDS             14899..15282
                     /locus_tag="LR110_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822296.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="LR110_001327"
     CDS             complement(15450..16208)
                     /locus_tag="LR110_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBL4822297.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="LR110_001328"
     CDS             complement(16226..17044)
                     /locus_tag="LR110_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL4822298.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="LR110_001329"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="LR110_001329"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBL4822299.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="LR110_001330"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="LR110_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBL4822300.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="LR110_001331"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="LR110_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBL4822301.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="LR110_001332"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="LR110_001332"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBL4822302.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         6008 a         4266 c         3844 g         6880 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaattt
//
LOCUS       DAFFSV010000040        20378 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFFSV010000040 DAFFSV010000000
VERSION     DAFFSV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.040"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            61..1485
                     /locus_tag="LR110_001333"
     CDS             61..1485
                     /locus_tag="LR110_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL4822303.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1784..2491
                     /locus_tag="LR110_001334"
     CDS             1784..2491
                     /locus_tag="LR110_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL4822304.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2478..2801
                     /locus_tag="LR110_001335"
     CDS             2478..2801
                     /locus_tag="LR110_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL4822305.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2890..3927)
                     /locus_tag="LR110_001336"
     CDS             complement(2890..3927)
                     /locus_tag="LR110_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL4822306.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4248..5231)
                     /locus_tag="LR110_001337"
     CDS             complement(4248..5231)
                     /locus_tag="LR110_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBL4822307.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5646..6416)
                     /locus_tag="LR110_001338"
     CDS             complement(5646..6416)
                     /locus_tag="LR110_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822308.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6409..7344)
                     /locus_tag="LR110_001339"
     CDS             complement(6409..7344)
                     /locus_tag="LR110_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822309.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7660..9309)
                     /locus_tag="LR110_001340"
     CDS             complement(7660..9309)
                     /locus_tag="LR110_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL4822310.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(9426..12029)
                     /locus_tag="LR110_001341"
     CDS             complement(9426..12029)
                     /locus_tag="LR110_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBL4822311.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12041..13090)
                     /locus_tag="LR110_001342"
     CDS             complement(12041..13090)
                     /locus_tag="LR110_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBL4822312.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13116..13430)
                     /locus_tag="LR110_001343"
     CDS             complement(13116..13430)
                     /locus_tag="LR110_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBL4822313.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(13446..13892)
                     /locus_tag="LR110_001344"
     CDS             complement(13446..13892)
                     /locus_tag="LR110_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4822314.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(13893..15815)
                     /locus_tag="LR110_001345"
     CDS             complement(13893..15815)
                     /locus_tag="LR110_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBL4822315.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(16027..16923)
                     /locus_tag="LR110_001346"
     CDS             complement(16027..16923)
                     /locus_tag="LR110_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL4822316.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(16916..17596)
                     /locus_tag="LR110_001347"
     CDS             complement(16916..17596)
                     /locus_tag="LR110_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4822317.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(17597..18379)
                     /locus_tag="LR110_001348"
     CDS             complement(17597..18379)
                     /locus_tag="LR110_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822318.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(18372..19289)
                     /locus_tag="LR110_001349"
     CDS             complement(18372..19289)
                     /locus_tag="LR110_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4822319.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(19453..19776)
                     /locus_tag="LR110_001350"
     CDS             complement(19453..19776)
                     /locus_tag="LR110_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822320.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         6011 a         4197 c         3424 g         6746 t
ORIGIN      
        1 ctatttactt taagttgtaa gcggtgttat cattaagtgg aaacaggagg taaaaaaatt
       61 atgacaacta tgaaaaagct aacttttcca gatgattttt ggtggggttc cgcttggtca
      121 gctgagcaag cagaaggaaa aggagaaaca ggaaaagcag agactatctg ggatcgctgg
      181 tttaaagaac aaccttaccg gttttatcaa cgaatcgatt cctctattac aaccgatcac
      241 atccatcaat ataaagaaga tatagaacta atgaaaaaaa ctgggcataa ctcctttcgc
      301 gtttctattt cttgggcacg aatgtttcca aatggcggta ccggaaaagt aaatcccaaa
      361 gccatttctt tctatcgaaa attattttcc gaaatgaata ataacggaat caaagtattt
      421 gccaacttat atcattttga catgcctgct actcttcaag ataaaggagg atgggaatca
      481 cgtgaagtag ttgatgctta tgttcattac gctgaaactt gttttaaaga atttggtgac
      541 ttagtttgtc attggtttac cttcaatgaa ccacttgggc ctattttagg tacttatctt
      601 gaagattttc attatccaaa cctcattgac tttaaacgcg gagcgcaagc agcattcttc
      661 actatacttg cacacgcaaa agcaatacaa gtatttaaag aactagctct tcctagtaaa
      721 ataggcgtta ttctaaattt gagtcctact tttcctcgta gtcaaaatcc tgctgatgtg
      781 aaagcagcag aaatcgctga tttattttat acgcggagct ttttagatcc tatggttaaa
      841 gggaattttt ctaaaaagct tatcctgtta ttacaagaat ataatcagct tcctgaagat
      901 tatacgaaag aagatcttgt atgtatctac gaaaataccg ctcaaattct tggactaaac
      961 tattacgagc cacgtcgcgt caaatctcgt tcttctatgg tggatacatc tagtccattt
     1021 ttaccagagt ggtttttcga accacatatc atgcctggac gacgaatgaa cgaataccgg
     1081 ggatgggaaa tttacgaaaa aggaatttat gatttgtgca tggatatcaa gaaccattat
     1141 ggaaacatcg aagcatttat ctcggagaac ggaatgggcg ttgcggatga agaacgtttc
     1201 atggatgata cagggcaaat aattgatgat tatcggattg agtatatcaa agatcatctt
     1261 gcttacttat ggaaggcaat aaatgaagga tgtaacatta aggggtatca tctttggaca
     1321 tttattgatt gctggtcatg gatcaacgcc tataaaaacc gatatgggct ggtttcactc
     1381 gaccttacta ctcaaaaaag aacaattaaa aaaagtggcg aattttacaa gcaattaagt
     1441 gatacaaatg gatttagata cgatgtagac ttgttagttg aataatattc taaaattcca
     1501 cttgaagtga acacattaga aaacaagaat aatgagaaat accaataaca aatacttttt
     1561 cttcatactt catttatact tgtcagtata agagactgtg acaaatgtag tgatcctcag
     1621 aagttaggtc tggaaatcta acttgagaag atttttttaa actacgtcca gtctttttta
     1681 tttgtttaaa attgaagttg acaagtcttc attaaaaatt tagaatattc acaagtttga
     1741 cgacaattat gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga
     1801 tataaaaact gcgatcaaag acacgttacc taccgttttt ggttatatcg gtattggact
     1861 tgcatttggt atcgttggga aagctgccgg gtttcatccg ctggtcgtca cgttgatgtc
     1921 cctgctgatc tatgctggtt ctgcccaatt tatcacggtc agcatgcttg ctagccatag
     1981 tccactgctc tccatcgttc tctcgacctt tctagtcaat tcccgaatga ttctgatgag
     2041 tatgacgatt gctccttatt tcaagaaaaa tcggctgctt caaaatctgc tgattggcac
     2101 attgctgaca gatgaaagtt tcgcactggg aatgaataaa ttgaactata ctggacaaaa
     2161 gttaaacttt cgttggatga atacagctaa ttggatttct tatttgacgt gggtgggttc
     2221 ttctcttgtt ggcgcacttt tagggaattt tattactgat ccgaaaaagt ttggtttgga
     2281 tttcgccatc gtcgctatgt ttatcgggtt actttatctt caagtcattt ctgatagaaa
     2341 cacctctaaa cgattacagt tgatcctgat tggtctcact ttgattctgg tatatgtcgg
     2401 actgattttt atcccaagta acttagtgat tttagttgta acactgattg ggtgcggatt
     2461 gggggtgtgg atcaaacatg ccttcttttg agtttacatt gctgactatt ttaggttgta
     2521 ctttagcaac ttggctttct aggatcctac catttgtctt actgaaaaaa ttcgatttgc
     2581 cgcaaccgtt gcttgagtat ttaagttttg tcccgatcgt cattatgtct gctttatggt
     2641 tcagcagtct atttacccaa aatatcggac atctgccgca gatcaatctc gaaaatgctt
     2701 tggcttcagt tcctacactt ttagctgcta ttctttcaaa aagcttattg gtgatcgtct
     2761 tggtaggaat tctttccctt tcgttgattc gcttattata aataaaaaaa caagagctta
     2821 tggcaaatta ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg
     2881 tattcttgct tatccgttga acggaccact aacaacgact tttcctaata tatgtcctgt
     2941 ttctacgtct ttggttgctt gtttcaagtt cttcgcattc atcccatctg aatagacctt
     3001 gctcaatgta ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc
     3061 tccttgcgtt tcgatttcat agttatagtc tgttttagcg aacatatact cccagtcgaa
     3121 gctgatagat tggtttttcc aaacacctat atctaacggc tctttgatcc ctacgatcgt
     3181 tcccacatgt ccaaatggtc ggacaaggtg tttgatttga tcaaagtagc gggtaatatc
     3241 gaataataca gcgacatact ctacttgatc gattcctaat tcacgaaaac ttttacttaa
     3301 gtcgtgatga taatcgattg gatagtctac acctgttttt ttcagccatt caaaattctt
     3361 cggacttgca gtcgcgtaga cttctaggcc agcccaatgc gctagttgat tcaaaatcga
     3421 accgacgccc ccagctccgt tgatcactaa gatttttttc ccgcggttgg cattttcttc
     3481 tggagtcaga ccaaattttt caaataacaa ttcataagcg gtcaatgagg tcaacggcaa
     3541 ggctgcggct tcttcatctg aaagattttt tggtgcaaga gcgacaatac gttcgtcaac
     3601 cagttggtat tcttgattgc ttcctgctct tgtcgttgta cctgcgtaaa aaacgcgatc
     3661 gccgacagaa aatttttgca cttcatcacc tacagcaaca actttcccga ctccatcaaa
     3721 ccctaagata cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg tatcgacagg
     3781 attaacagat actgcgttga cttttaccag aagatcccgt gaactgggta caggtatctg
     3841 ttctttttca tcaataaagc tttttggatc atcgatcgac aacccttcat aaaaaccgac
     3901 taccttcatt aattcctttt ctgacatacg aattcctcct tagcttcatc aagttggagc
     3961 atccatacag ttttcatttc aatatatata gtatgctatt tgttccactt ttattcatca
     4021 taattattct cttacttaaa gtaaactatt tgcacttttt tggttaaaaa agaggttgcg
     4081 acaagacttt tgtcacaacc tcttttaaac ggtgttcaat caactgaccc tcggtattcg
     4141 ctcaaaaaac gacaaaatat gaggagctat ttttgtttgg ctgttcttat tacttgatgc
     4201 aggacagctt tttcacgacc tctaattcac ggtgttcaag caagaaatca ttgttcactg
     4261 aaaagcgttg gatgttcttt ttcaaaggtg tctaagatct ctcgtacttc atcagtagat
     4321 ttcgtattca tcaactgaac tcgcaagtca tttgctcctg gaaatccttt gatatagatt
     4381 ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga tttctgaata atgatcttgc
     4441 agatctagtt gcagacgaag tagtccgatc agttcttttt ctgtatgttc cctcggttct
     4501 ttttcaaatg cataaggatt cttgaaaatc ccccggccaa tcatgatgcc atctacgcca
     4561 tatttttcgg ccaattcttc tcctgtctgg cgatctggga tatctccgtt gatcgtgatc
     4621 agtgtttgcg gagcgatttg gtcacgtaaa gcaatgattt tagggatgac ttcccaatgt
     4681 gcatctactt tactcatctc tttacgcgta cgcaaatgga cagaaagatt ggctatatct
     4741 tgatgaagta ggtgactgat ccattcttcc atctcagaca tttccgtata gccgatacgc
     4801 gtcttgacgc tgacaggcaa accgccagct ttagccgctt caatcaattg tgccgctact
     4861 tctgggcgaa ggatcaagcc actgcctttt ccacgttctg ctacattagg gactgggcag
     4921 cccatgttga tatcgattcc ttggaagccc atttctgcca tagcaatgct catttctcta
     4981 aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaag
     5041 gtcaatcttc ctcgtacact gtcttttcca tctggatgac agaaactgtc tgagtttgta
     5101 aactcagtaa agaagacatc cggtgctgct gcatgcttta cgacatggcg aaatacaaca
     5161 tctgttacat cttccattgg tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa
     5221 ttagtagtca tttatttgtt actctccatt ctatcaagca gtgaagtcgc aatctttctc
     5281 aaaaacattg tcattatact aggaaacaaa aaaagaagca aagtgaacag cttaaaagat
     5341 gtttctgatt gagcaaaaaa agactgtgac aaaaatctta tcacaattcc ttttccggaa
     5401 taaatggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaaacga aaaataggag
     5461 gagctatttt tgtttggctg ttcttattac ttgatgcaga acagcttttt cacaacctct
     5521 ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaacaaacg acaaaatatg
     5581 aggaactatt ttcccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct
     5641 cttttctaac ttctcaatcc tcttccttta ttgatcaaat accaagtaac ggcgtacaaa
     5701 acaacgatga atagcacaag tacgatcatc gatgttgcta ctggcacatc gattgttcca
     5761 aggaatccgt aacggaaacc ggagatcata taaacgatcg gattgacttt cgaaacagct
     5821 tgccaaaatg gcggcaacat agagatggca tagaaaacgc cacctagata agttaacggc
     5881 tgcaagacaa atgtaggcac gatcgataca tcgtcataag actgagcaaa aatcccgttg
     5941 agcagtcctg ctaacgaaaa caagattgcc gtcatcaata atgtaatgat gaccatgaac
     6001 catgaataca catgaagtgg tacaaagaac aaggaaatga tcgttacgag agagccgacg
     6061 agaatactgc gacccactcc gccaatcaca aagccccaaa tgatcacatg cgtaggaaca
     6121 ggcgctacca agatttcttc gatgtttttc tggaattttt gagagaagaa ggacgaagaa
     6181 acgttggcat acgaactagt gatagcagac atcataatca aaccaggcac gatgaattcc
     6241 atatatgaaa aaccacccat gtcaccgatc cgaccaccga tcatttttcc gaaaataaca
     6301 aagtacaagg atgtcgtgat cacaggcgga accagtgttt ggacccagat acgcaaatag
     6361 cggttcgttt ccttgatagc taaacttttt aatgcagtaa aataaagatt aaacattttt
     6421 ctcctccgta tgattatttt cttctgtgat tttcaaaaac agttcttcta aacgattcga
     6481 cttattcctc atagaaagaa ctttgattcc ttgtcttgtc agctgatcga agattccgtt
     6541 caccccttga tttcgttcaa cttcgacagc gattgtctgt tcatcttcaa atgtacaagc
     6601 gtatccttca atcacaggtt tggtctcata tggcgctaaa tcaaagataa atgtttcaaa
     6661 ttgcagtttg gatagaagct ctttcatact agtattctcg atcagttcac ctgattggat
     6721 gatcccgatg ttgcgacaaa gcatttcagc ttcttctaaa taatgcgtcg tcaaaatgat
     6781 cgttgtacca ttttcgttca attctcggag aaactcccac atctcgcgtc tcaattcaat
     6841 atcgactcct gctgttggtt catccaagat cagcaaacgt ggttcatgca tcaatgccct
     6901 agcaatcatc agtcttcgct tcattccccc ggaaagcata cgtgcacgga catttctctt
     6961 ttcccaaaga ttggactgct tcaaatattt ttcgctgcgt ttcatcgctt ctttgcgaga
     7021 gacaccgtag tagcctgctt gattcacgac gatttgctgg accgtttcaa agggattgaa
     7081 attgaactct tgcgggacta atccaatctg ttgctttgct cggactagat ccgtatccag
     7141 atcataaccg aagacattca cttttccaga tgttttattg actaaagaag taatgattcc
     7201 gatcgttgta gacttcccag ctccatttgg tcctaatagc gcatagaaat ctccttcttt
     7261 cacggaaaga tcgattcctc gcaatgcttc gaccccactt ccataaacct ttttcaaatt
     7321 atttatttca agcgcatttg ccatttattt cctatctcct tatagacatg attctttatt
     7381 gtatcatgga atacagcaga tgaaaaaaca acttggttcc agcgaaaaat caggttgctg
     7441 ggctcaggaa aatctcctaa tgaaagacat caatcggacg aagtcagaca accttttcag
     7501 aaaaaacaaa tcgaagataa aggattacta ttcatatgtg caacaaaagg agcgtgagac
     7561 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcaaaagc
     7621 caagattttt gatccaataa atactttcga tcacttcgcc tatcccaata catgccactg
     7681 ccgcttttca tcaaataacg cggcttccgt ttttgcggtt tctactaaag tctctgggta
     7741 atccaaaact ttcagcaagg caatcgcatt tcgggtcctt gaaggtcctt cttttaattt
     7801 gaaatcaaaa ctgattcctt ttccttctgt tacttgttct tcaaagtgga cattctcaca
     7861 ataattcttt aaaatctctg tcaattcgat atcatgtgtg gcgacaaatg ctaaagatgg
     7921 atactccgca agccaccgaa cgactgaagc ggaagaagca atccgttcga tcgtatttgt
     7981 cccttttaaa atctcatcta cgaagcagta gcagcgttct ttggaagcca cttggtccaa
     8041 caaacgtttg atcgatttga tttctgaaac aaaataactg tcgccttcaa ataaatcatc
     8101 ttcgattgcc atggaagtca acacatgtcc aggttgcagg gtaaactgtt ctgcaatcgc
     8161 tgtttggatc gtctgtgcta gaatacagct gatggctata cttttgacat acgtagattt
     8221 tcctgatgca ttggaacccg tgactaatgt attcttctgc cagttgaccg gattcactac
     8281 cgcttctttt aacaatggat gatagatatc tttcgctgtc acaccgccct cagtaaactc
     8341 tggtcggcaa gtgatgggca tataagtacg gtaattcaat attgcagcgg ctacttctaa
     8401 ttctcccagc aattcccaca gcttgatggc ttcagttgaa tgcttttcta atttagtgag
     8461 aacgaaatga taggagataa atggcagcat gaacatgatg ttgacatatt caaaaatcaa
     8521 ctctgcttca ctcccactct tcatacgaaa tgaaaaacct aatttaggga tgtctcttaa
     8581 tggtttagtt tgctgtttca gctcttcttg aagtggtgtc ggcagttttg ctagctgatt
     8641 ggcggctgaa atcgttgaaa ccaaataact catgctattc agctctgttt ctagtgtttg
     8701 tttcttcact agataataga ccgtattgaa tactaaccca ccgattgcca aatagattcc
     8761 tagtacttgt cctaaaagaa gcaaacccaa acccaccagc ggaagcaagc caagaaaaat
     8821 aaataaacct acgcgtccaa tctttttact ttttccatcc gctagatatt gttttgcaaa
     8881 attatgatct ttttttccta aacgagcgaa ttgatattgg attttttctc tcagttgagg
     8941 attttcctga taaaaagcaa tcagtttttc taactgctga tcttcaccaa aatcaaagct
     9001 gcgtaaacgt tgatacaacg cttctgaacc tacactagaa taggtactgt tgattccttc
     9061 gaatacttcg aacatatcca ggtcatacca agtaagatca tcgatttcgc tatcccatga
     9121 acggaacttc ttctccgtga gccatgcttc cttcaagctt tcttctttat caaatctcgg
     9181 ttgataagga attttttccc atttgctacg aactgtttcc tttaatttca aacgtgaaaa
     9241 aatgtctaaa gcaactaata aaataacagc tgcaataata attaaaacga taaattctct
     9301 ttgacccata tccctctccg ttcctttatt tgttagcttt agtatagcac tgttgcgcta
     9361 tttgaaaaga gggtaaaaaa acacctttca aaataaaagg tgtttttttc ttgtatatta
     9421 catatttatc tctcatttat caaaattgac aaataatcat agggaggaat tgttgttaat
     9481 tcttgtttat tctccagtgc attgacaact gtcccaaggg aaaggatagc ctcaggtact
     9541 attttttctt gttctgttgg attagctaag cgaagaacaa atccttgttc atgaaaataa
     9601 gaaggataaa tcgatgatac aattaagtct tcaggtaatt caatcaatga aaatttacga
     9661 ggaacatcaa gttcattttg taatggccaa atcttgttat ctaacctgtg aataaataca
     9721 ttcagtttct gcttttgata agaaatagat tgttctaacc gtttttctgc ctgttttgcc
     9781 actttgaact cattaaatct tccttcttct acattaaatc caaaagtgac tttatactca
     9841 cctatctctt gagctagcgg tgtttccatc atgacatgcc cttcatttgt cgtatctcct
     9901 gaagctcgcc caggtcgcca agcaagatta ggttttccaa gttcccctgt tgttgaaaat
     9961 aaggttatga ttaattgatt ttctatacgt tcatactctt ttaaaccatc tgcataaaac
    10021 gtaaaataat gatcattttc tgccacagag acactcttat cgaatatttc taaattaaca
    10081 ggtttctcta ccatagtttc tgaccaattc ggaggaatga catcaggggt attttcaata
    10141 aatccgtttt gaatttgtgc aattgatttt tcagatttat ctaaaacatt aattgctaag
    10201 cgcatacgat gcgagtaaac agtattatga attttgagtg tgccttcaac gatttgactt
    10261 tctcccagac tgaacgatat ttcataagat atttcttcca tttcgggttt ttcagataaa
    10321 cgatctttta aatctttagg aagttgagct tttccgtaaa caactaatgt ttcttgtaaa
    10381 gaatctttat atactttagc tgtttctaat tttagagata gttctgtatc tccttctaat
    10441 ggtgaataat catacgtatc gccatcattt gctgaatcga tcaaatgaac gaaatcaaga
    10501 tattgtctat ctccaactat caaattcaat tgcccatctt taaaagaaag tttatattta
    10561 ttgttactta tctcagtttt gtctatctcg attgctgttt ctaatttttc attatttttc
    10621 tcaaattcaa caacagtgta tcctagtgct ggcacattta catttatttt tatgtcaagt
    10681 tcataataag ctggttcatc aaaatattcg aaacctgtag caatcatccg ataagcattt
    10741 ttccgttctg gaaaataaac ttctttttca atgatattat agggactacc tttgaagtgt
    10801 attttctttg aatcagctac tacatgcacg attttcgatc cactataatt ctcagtcgtt
    10861 gtgttgaaaa caaggacttc attcgatttt agctttaatg catcagccat ccgttttaca
    10921 ataaggtttt cgattccgtc agctatctca ttagcttctt tgattcgatg gaaaatatct
    10981 tccgcaacat tatctgaaac acagccacca atactatcat gagcctggtt ttgcaaaatc
    11041 tttttccata accgacgaag taggccttta ctgatatgga taccatttct ttctgcaatc
    11101 agcatgagcg gctcaactcg ttttagaatt ttttgttcca atttaaaatt ttctattttc
    11161 atttttgttc ttactgagcc aatagaacga tgaacacgag agtaaaccgg ctgacgaagt
    11221 tcacctttat attcaggtaa ttcttttttg tttctgataa cttctacata agaaggataa
    11281 tctgaaataa tattgtggaa tttactgtat ttattcaact tctctacgac ttgatcaaaa
    11341 tttaaaatca ttgacttttg atctacaccc gaaggtacta aaatatcttt gttttttcct
    11401 tgcatatgaa gcatttcaac tgcttcatcc aacttttctt ttgcaaattc aggaatatct
    11461 ttaattgcgt ctaaggtcat cattccagtc atataaccaa atggaaaatt catggcataa
    11521 acaaatttat ctcccaatcc tttccagata aaataaggag atggaaccat cgtttcaaag
    11581 tttaatcctc gccaacacat gaaattatcg atttctactt gatgcaaaag tgtaggcaaa
    11641 ttcgcattga acccaaacgt gtcaggcaaa taccctacca tcattggatc tccatattta
    11701 aggatcgtat cactgattcc aatcattaag tttcttaaaa ttgattcagc atccactaat
    11761 aaggcatctg tttgtgcata ccacggtccc acgaatagtt gtcctgtttt catcagtcgt
    11821 tggataacct taagcttttg cggattgatc tccaaatact catcgacgat tgaagtttgt
    11881 ccatctaaac aaaagtttac tttagggttc ctttccaatt catctagaac ttcagtaaag
    11941 acctgatcag acaatacgat ggcatcttca tttgtaaaat accattctct gtcccagtgc
    12001 gtgtgttgaa ttatatgtgc atttaccata tcagattctc ctactttcct tcttctactg
    12061 aatttacttg ttgttctttt ttatacttga tggattttag tgcattgacc attaacgctg
    12121 taaccactcc cccaataaca attgatacta aatataaagg tatattcgta acaaaaggaa
    12181 ttgccataaa ggttgaaagc attgtttctt gagtaacacc aaaaaatcca tttagaccac
    12241 atgctactgc agtgcccacc acgatgcttg gtattactcg taaaggatct ttcacggcaa
    12301 acgggatcgt cccttcagta atccccacaa ggcccataat catagctgtc tttgcattat
    12361 ctcgttccga ttcatcataa agttttttgt ttaaaattgt tgctaaaaac attccaaatg
    12421 gcggaatagc aattcctaat aacatggctg tactaggtcc aaagcttccg cttgaaaaag
    12481 aagcaacccc aaaagcatat gcgattttat tgataggtcc tcccatatca aacgccatca
    12541 ttcctcctaa aatagccgac atcaaagcag catttgctcc tgacatgcct tctagccaat
    12601 tagtcagtcc tgttgtcatt gcttgaatag gcgtagctag tacataccat aatatgattc
    12661 caacaactaa tgtagtaaca aaaggaatca gcataattga ttttagacta ataacttgca
    12721 tcggtacttt tacttttttc aatgcattag ctaaatagcc acaagctaat ccagcaacca
    12781 ttcctccaat aaatcccata ccaagatttt gacacatcat cccagtgata aaagctggtg
    12841 ctagaccggg acggtcagca attgaatatg cgatataggc tgaaatcatc ggtacgataa
    12901 actgtaatcc cgtctgacca atcattcgca ggactcccca gaatgtatcc gtctggtccc
    12961 aaacacctgt gccaccaaaa atgactgcaa tagaagttag taaccctgct gctacgacaa
    13021 gcgggatcat ataggaaacc ccactttgga aatgatttag aatttctctt ccttgtttct
    13081 tgaattccat tagtaactcc cccacgatat attttttatt tgttaattaa tgcaattgct
    13141 tcatcaatcg tctcttcacc atcttttact acctctgagg tccctactgt taaaatcggt
    13201 ttaccgtcaa agcgacctcg ttctgaaaca tttgtatcta ctgcaaatat gacaacatct
    13261 gcctcggaaa catccgaaat tgaaagtttg ttttctatcc ccatagcccc ctgtgtttct
    13321 actttaatat ctaacccttt cttcttcgca aacttttcta aattagcttt tgccatataa
    13381 gtatgggcaa tccctgcgat acatgctgtt actgctacta tttttgtcat atgaaattcc
    13441 tccttttaaa atgatagagc ttttaccaat tcttttttat ttctcgaatc ttttattctt
    13501 ttaacaaact cgtcatccat caatcttcca gataattctg ctaaaatacg taaatgatta
    13561 gtacctttat cagtgtcttt tattgctaac aaaaagacga gatcaactgg ttgatcatct
    13621 aatgcgcccc attcaacaga ccgctttaac ttggctacag ccactgttga ttcaattact
    13681 gctaggcttt tgccatgagg aatggcgata tttttcccaa ttcctgtggt catatggttt
    13741 tctctgtcaa atacgtcttt ttgaaattgt ttgtggtcag acacaaaccc attagtctct
    13801 aacaatttcg ataatttttc gactacactt tttttatcag atgcatttaa atctatcact
    13861 atattattta tattgatttt ttcactgatc atttataagc cttctttcaa tatataaact
    13921 aatttttcaa acgacgaact cgtttttaaa tttgctaata actcatgatt cgaagcgatt
    13981 tcattgaatg tttcataaat tttattcaat tcaggtttta ctttttcatt aagtgcaaaa
    14041 aagaaaacag aatgaacaag tgtcccatcc caatcaattc cttctggatt aacgtagaca
    14101 cagattactg gcttttttat aaaatctact tcgatatgcg gcatagcaaa atcattcatc
    14161 gctgttgaag atagtttttc tcttttgatt gctccctcta gtatcccttc tttcgcgtaa
    14221 ttttctttta ccaatttttt ggtaattata tttaaaacgt tcacttgatt atcctttatt
    14281 tggtgggtaa aaaagcaaaa tcgtttatct aataaattta gaaaagcatc ttctgtttgt
    14341 tcatttaact gtagatactg ttttactttt ctgacatctg cccctaacat caaaggacta
    14401 acaacaataa caggaaaatt tgtattttca atcggaatcg tgcttataac aagttcatca
    14461 gtgacttttc tatcttgtag ttcattgatg cttagtacat cttctatctc aaccatatct
    14521 gcaaaaccgt tatttattct ttgctctaat aattttgatg ttccatatcc gctactacaa
    14581 actaatatca ctttcgtttt gtcaggtttg taacgatcca aaagtgattg aatatggagt
    14641 gttacgtaag cgatttcatc ttcattaaaa tgtatacaga atctctcttc gatttcctct
    14701 aataaatcta cacttatcat aaatgacctt ttaaaagaat atttgatttt gtccgtataa
    14761 gggttataga tatttactcc caatttgagt cttttaactg ctgcattgag atgtaatact
    14821 agattattga tcaattcttc atcaggataa aattcattta atttaattct tactaactct
    14881 tgtagctcaa cacccatatc taaataggaa agcttcgttt gatctaagtc gtgattcata
    14941 gcattttttt caatcgaagt aatgtgaatg tcaacataag cttgttcatc tattggcaca
    15001 ataattgaaa atgctttttc aatcattttg attaaaataa gtgtattctt tgagatttct
    15061 tcttcagaat tattactacg tgataagaaa ttgcttgttt caatcctttc tatcgcaata
    15121 gcaaggtgga tagctaaagt ttgaaattca taatctgtaa atttcaattg tgtcttattc
    15181 atgtatcgat taagcaattc ggctactttt ttcaatatgt ccttatttaa gaaattattc
    15241 gcttcaccaa tgaaattgat attcaagtct aagagatctg ttttactgtt agatgctgta
    15301 aagccacccc aataatagtt cacaacttct gacatcaacc ttcttttact tttttcatca
    15361 gctttgatgc gcacgccatt tcgatctgaa actaaggaca tcccatgctt gtttagtctt
    15421 tttctaacat ctttcattgt gctttctacc gtcgacctgc tcacgtataa ttcgtcagca
    15481 agattttgaa tctttagaga atcattagta ttcaataatt tagcaaacac ataagttaga
    15541 cggtcctccg gagagtctgc gatattgatt ttatgactgg ataataaatt tcgaagtgtt
    15601 tctttttttc caataagcga aacgcctttt ttaggttgaa ccaacatttg tacatctggc
    15661 attttttgga ttatttcttt caaaattaaa atatctttgc taattgtttt cgtagataca
    15721 tataattgtt ctgataattc tttgtaagtt gtgtgctcat tttcaatcaa aaagtatagt
    15781 attttcattt gccgttcgct aaattcgatg cccattatct tcacctcctg aatacagctt
    15841 aagtgtacaa ttattatctc tattttcata cagaagaaag ttcttgatta atgtagaagt
    15901 ttctaatatt cttgattatt tcaaatcaat aaaaaagcct gcaatattta tatcacaggc
    15961 ttttttctct attaaatata tataagataa tacatttttc tactagttct cactaatctt
    16021 gtataattaa accctccgca acttgacgga gatggagaag atgcccccgt ctaatctagc
    16081 ggtcatctct cctcccattc cttccaccaa tttttttgac aggtaaagcc cgattccgct
    16141 agattccgta tttgtgcggc tggtattttc ggtataaaaa cgctggaaga ctttttcaac
    16201 agaggtttgc gttcctggtt tgataggatt ggccactgtt aatcgaatga atggatcctc
    16261 ttttgaaaat gtcacctttg cttgtccatc tgcataacgc aataaattct gtatgatatt
    16321 ttgcagcact cgcagcaaca tgagcggatc tgctaataca gttgtattta tcgagtgttc
    16381 agcgaacgct acctcgatat ttttctcaga aaattgttca taaaaagaga aaaatacttc
    16441 ttccacctct ttagaaaaag aaagcgtatg gagatccatt tctttttgct tcgtctctat
    16501 tcgagcaaga tcataaaact gatccgtcat actgagtaat cttgcaacag attgtttgat
    16561 gatcgttaaa tacttctccc gtttctccgt atcctctacc tgttccagta attgtacata
    16621 accgttaata gcagtcaaag gagttctgag atcatgagaa aggccgacga tcgatagtct
    16681 gatgttttct tccatttctt gaaatgcttg tcctttttgt tcattttcat ccaaccatgt
    16741 attcagtgca tctactaggt ccattagatt cttttctcga aattcgactg aaagacgacc
    16801 accatattct tgtcgttcag aaagagtggc gattttctta gttaatctct tgatttccct
    16861 ttgcaggaag aaaataaaac ttcccataaa gataacaacg agaagcaaca gccatttaat
    16921 caaatctaaa ccccactccc cacaccgtat gaatatacac agcatgtgcc tttgctagct
    16981 tccctcgaag attggaaata tgcatattca ctgttttctc actatcaaaa tatggttctt
    17041 gccaaactgt ttcataaaga tttgctcgcg aaaatacttt tttcggatgt tctaaaaaaa
    17101 gcttcagcag atcgaattct cttcctgtca aatggacagc cacaccattc acatttacat
    17161 ccctcgactc tttgtccagt aagatttcct tgtaatagat gcgttgcgac agatctttta
    17221 cttggacaga agtctgatga cgaagttgga tgccgattcg taacaaaagt tcttcgatat
    17281 caaatggttt cgtcacatag tcatccgctc ctgctgccaa caaggaaact ttatctcttt
    17341 gatcgctttt tgctgaaaga acgatgatcg gaacattcga tgtctgtcgg atttctgcta
    17401 acaatgcttc acctgaaagc cctggaagca tgagatctag aaggatcaaa tcgacaggat
    17461 atgtgcttaa caataatttt ccttccgtac ctgaataagc atcaagaagt ttgtatctct
    17521 gttcaagaac ctcctccagt aaggaatgaa cagcttcatc gtcttcgata atcaaaatag
    17581 tttccagcag gaccacctac ttaatgtcag atttttggaa aataaacatt cctagagctg
    17641 atgataccac gattgttact agactgacca gaatcatttt cggtgcttgc gaataagttg
    17701 cttgttgcgc aaagtccgta tacatgagcc acgtttccgt aaaccactcc gttagaaatt
    17761 ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc cgttcccatg atataaagaa
    17821 aagaaagaag caaactgaca ctggtacttc tagtgatgaa tgtcaaagcc actggaacag
    17881 ataatgtccc taatgtcagc agaagcaata caggaaatac ttttagaaca taatttccat
    17941 agtttttgat tccctcaact tcaccaaatc cgaaagcaag tgtgtaagca agcatggcta
    18001 gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag cgtaacgaga agtttcgaca
    18061 cataataagt cattcgattc cgtcctaaag ataacgtatt gcgaatcgta ccgttttgaa
    18121 attcttcccc aatataaaag cacgcaaatg ctgcgaaagc cagaagaatc catgcagcca
    18181 ttttttctac cattaaaatc cctgttgcac cgttgatgac tgtatccgtt gcttggttta
    18241 attcattgcc gtattttgta tgcatatacc attcgttcca agaataaaaa agcggaagta
    18301 cgaccatcag tcctagtgag acccacattt tacggctctt ccatactttt attttttcag
    18361 cagttaatag attaaacatg tggattgcct ccagttaatt gtaagaaata atcttccagt
    18421 ttttgcccaa ctaggtgaat accatccaca ggaacttttg cttctaccaa tgcttgattg
    18481 atggctgcta cttggtcaat tccttcaaaa agctggataa cacggctaga ttgtacaaaa
    18541 taatccctat aaccaagaga gtcgagaacc gttactgctt gttccgtcgc cgaagtgtca
    18601 atttttataa attgacgaga ttcttgtgcc aattcttctt tcgataattc tttgatcaag
    18661 tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg acaattcatc tagcaagtgg
    18721 ctagaaagca agacggtgat gccccgttct tttaccaaac gttggatgat ttctcgcatt
    18781 tctgccatcc ctgctggatc catcccattc gtcggttcat ccaaaatcaa aaattctgga
    18841 ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca tacctaaaga gaaattcttt
    18901 gctttctttt tccctgtatt ttccaatcgc atcattttca gcaggtcttc gatttctcta
    18961 tccgacacac cgccgttcgc tgcttggata cgtaggttat ctcttgcagt taattctgga
    19021 taaagagcag gggtttcaat cgattgaccc atacgtcgtc ttgctgcctg caaatccgtc
    19081 gttccaaata attcgatcga accttcatca atcgagatca aacccataat agttctcata
    19141 aaggtagact tacccgctcc attttcccca atcaatccat agatcattcc acgtttgatt
    19201 tcgattgata ctttatctaa tgctttggca gcaccgtatt ttttggttat accagttgct
    19261 ttcaagaccg tttcatttcc aaaatccata cttatccttc tttcttattg atgagtgaag
    19321 tatagttgac gggtataaag aaagcagcca gaaatgtata aagaaagtgt aaattttcca
    19381 attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt cctattatca ggcagtttgt
    19441 tatacgcttg tatcagtttc ctgattcttt cttttcgacc aaagtcgttt ctctgtattc
    19501 ctcttcactg aaaatttttg cttctttcag atcaagcttg agtaatttta ccagaccggt
    19561 tataaggata actatagcaa gactccctcc cagaaaaatt agactgtctc gcagacgtgg
    19621 ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct aatccaagtg gactggccac
    19681 catggtaatc gttgttatcg ttgacataag tcgtccttgt atatgcgttg gaacttctcg
    19741 ctgaagccag accatataaa aagcaaaaga aaacattaat ccttaaaaac ggacttatga
    19801 aaaataatac atgccgtttg ggatttacaa ttagctacca aataagaggt tatgacaaaa
    19861 gacttttcac aatcccttct tccgaataaa cggtgttctc ctgccaactc ctcggtcaca
    19921 agtcacaata tttatcaaac atgagaagca atttttgtaa attccttctt atttcttgaa
    19981 gctgaccact tctgtcacaa cctcagaagc ggataaataa tattgtttga tgatacttta
    20041 tctttttccc gtttgtttat agatcgaatg atattataga ccaagaatcc tacctattaa
    20101 catagctaaa aacacaaaaa aggcactttc caaattgtgg aaagtgccag aaaagttagg
    20161 attaaggtaa ctgcgctcag cattgcttcg cccagtacga ttctcgtaac tcgtgaactc
    20221 gaacgataat cgcaattaac gttttgagaa ctgtgaagct ttacgggctt tcttaagacc
    20281 tggttttttc ttcgtatcat tgaataagtt tgaaaagcct tgatttataa aggtttttcc
    20341 gtaaatatat tttgttatat ttgattaaat ttgaaatg
//
LOCUS       DAFFSV010000041        20362 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFFSV010000041 DAFFSV010000000
VERSION     DAFFSV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.041"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            292..1431
                     /locus_tag="LR110_001351"
     CDS             292..1431
                     /locus_tag="LR110_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBL4822321.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="LR110_001352"
     CDS             complement(1498..2073)
                     /locus_tag="LR110_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL4822322.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="LR110_001353"
     CDS             complement(2078..3052)
                     /locus_tag="LR110_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBL4822323.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="LR110_001354"
     CDS             3335..4303
                     /locus_tag="LR110_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL4822324.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="LR110_001355"
     CDS             4416..4694
                     /locus_tag="LR110_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL4822325.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="LR110_001356"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="LR110_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBL4822326.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="LR110_001357"
     CDS             5151..5906
                     /locus_tag="LR110_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL4822327.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="LR110_001358"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="LR110_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBL4822328.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="LR110_001359"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="LR110_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBL4822329.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="LR110_001360"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="LR110_001360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBL4822330.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="LR110_001361"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="LR110_001361"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBL4822331.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="LR110_001362"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="LR110_001362"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBL4822332.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="LR110_001363"
     CDS             10643..11185
                     /locus_tag="LR110_001363"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBL4822333.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="LR110_001364"
     CDS             11208..12764
                     /locus_tag="LR110_001364"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBL4822334.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="LR110_001365"
     CDS             12778..13680
                     /locus_tag="LR110_001365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBL4822335.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="LR110_001366"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="LR110_001366"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBL4822336.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="LR110_001367"
     CDS             15123..15545
                     /locus_tag="LR110_001367"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBL4822337.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="LR110_001368"
     CDS             16014..16244
                     /locus_tag="LR110_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBL4822338.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="LR110_001369"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="LR110_001369"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL4822339.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="LR110_001370"
     CDS             17655..17831
                     /locus_tag="LR110_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL4822340.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="LR110_001371"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="LR110_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBL4822341.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="LR110_001372"
     CDS             18326..18709
                     /locus_tag="LR110_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4822342.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="LR110_001373"
     CDS             18878..19762
                     /locus_tag="LR110_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL4822343.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="LR110_001374"
     CDS             19876..20112
                     /locus_tag="LR110_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBL4822344.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="LR110_001375"
     tRNA            20200..20280
                     /locus_tag="LR110_001375"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="LR110_001376"
     tRNA            20287..20358
                     /locus_tag="LR110_001376"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6760 a         3566 c         4425 g         5611 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta ta
//
LOCUS       DAFFSV010000042        20185 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFFSV010000042 DAFFSV010000000
VERSION     DAFFSV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.042"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            106..402
                     /locus_tag="LR110_001377"
     CDS             106..402
                     /locus_tag="LR110_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBL4822345.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(1046..1999)
                     /locus_tag="LR110_001378"
     CDS             complement(1046..1999)
                     /locus_tag="LR110_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4822346.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(2022..2777)
                     /locus_tag="LR110_001379"
     CDS             complement(2022..2777)
                     /locus_tag="LR110_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4822347.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2774..3733)
                     /locus_tag="LR110_001380"
     CDS             complement(2774..3733)
                     /locus_tag="LR110_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL4822348.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3726..4676)
                     /locus_tag="LR110_001381"
     CDS             complement(3726..4676)
                     /locus_tag="LR110_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL4822349.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4826..5278
                     /locus_tag="LR110_001382"
     CDS             4826..5278
                     /locus_tag="LR110_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBL4822350.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5367..7634)
                     /gene="gshAB"
                     /locus_tag="LR110_001383"
     CDS             complement(5367..7634)
                     /gene="gshAB"
                     /locus_tag="LR110_001383"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBL4822351.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7821..8819)
                     /gene="ftsY"
                     /locus_tag="LR110_001384"
     CDS             complement(7821..8819)
                     /gene="ftsY"
                     /locus_tag="LR110_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBL4822352.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8830..9639)
                     /locus_tag="LR110_001385"
     CDS             complement(8830..9639)
                     /locus_tag="LR110_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4822353.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9648..13229)
                     /gene="smc"
                     /locus_tag="LR110_001386"
     CDS             complement(9648..13229)
                     /gene="smc"
                     /locus_tag="LR110_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBL4822354.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13249..13935)
                     /locus_tag="LR110_001387"
     CDS             complement(13249..13935)
                     /locus_tag="LR110_001387"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBL4822355.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14283..16064)
                     /locus_tag="LR110_001388"
     CDS             complement(14283..16064)
                     /locus_tag="LR110_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4822356.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(16090..17004)
                     /locus_tag="LR110_001389"
     CDS             complement(16090..17004)
                     /locus_tag="LR110_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822357.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(17031..17993)
                     /locus_tag="LR110_001390"
     CDS             complement(17031..17993)
                     /locus_tag="LR110_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822358.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17993..18937)
                     /locus_tag="LR110_001391"
     CDS             complement(17993..18937)
                     /locus_tag="LR110_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822359.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18938..19942)
                     /locus_tag="LR110_001392"
     CDS             complement(18938..19942)
                     /locus_tag="LR110_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822360.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5637 a         4312 c         3578 g         6658 t
ORIGIN      
        1 cctttaacaa ctattggaaa aagaattact ttttgttcac ggattattaa caactcttcg
       61 gtagagtaaa cttatcaaat aaaagacttc tttggaggaa tcactatgaa ggaatatata
      121 aaaaacatct attttatcga agaaacacaa aatatcgagg gttcttatat tgaagtaaaa
      181 acactctttg tcaatgaaga taaaaccaaa gcattggata tttataaaaa attagcttca
      241 aaaaaaacga atagttttgg attgatatta agcgagtata aaatcaaagc agaagagagt
      301 tatttctatc aattactaaa acggtggagt aagcttcccg ccgattttta tcgaaaaatg
      361 cagatcatca actatcaacc gcttgctgag acacatgcct aaatagaggt tgtgaaatca
      421 gtgctttgag ctaataccga aggtcagctg attgaacatt gtttagaaga gattgtgaca
      481 actgcgattt gctttgagta ataagaacgc tggacagaaa atagcttctc atattttcta
      541 gtctagtggg cttattgccg agaagcagca gatggaacat cgtttagaag agattgtgac
      601 aactgcgatt tgttttgagt aataagaacg ctggacagaa aatagcttct catattttct
      661 agtctagtgg acttattgcc gagaagcagc agatggaaca tcgtttaaag aagagatcgg
      721 tacaaaataa aaatatctaa atttaagttt tatctaagtt tcgctttgta cctttgattc
      781 aaatcaaagg aggcgatctt gatgctgaaa aaatggatca cactattttt tgccactccc
      841 cattctagaa tcaacccgta tgatgtagat aatgaattca cgttgaaaaa agatttctca
      901 aataaataaa aaccatccgt aaaatcatca atacgatttt acggatggtt tttgataact
      961 tctttgagga taggctcttg ctatttaaga atgtaagaaa cattattgtt acatagtatg
     1021 agagtttaat cctctgttat ttatgctatt taaatgcttg attcacatct tcgatcatca
     1081 gtttcattga ttctaaaccg cccccgctta aataccagac atctggttca agggagatga
     1141 tctgctgatt tttaccagca ttcgtttgtg ctaccagttc attggcagaa atatcatctt
     1201 tgctgtcgtc tccaccaata gcttttgttc tgtctactac aaataagata tccggatttt
     1261 tttccagcac atattcataa gaaacacttt gaccgtgagt ggatgcttcg atttgatcat
     1321 ctgcttgttc aaagccaaat aaatcatgga tgaaaccgaa tctagaacca gatccgtagg
     1381 ctgataattg cccttcattc acaagtgtga ctagtgcttt tttgtcagta gcttctgctt
     1441 tctttttcgt ctgatcgatt gcactagata attcctctaa ttgagtttta gcttcttctt
     1501 gcttaccaaa gatctcccct aaagcagtga cattttgctg gaaagattcc catggatttt
     1561 tcgcatctaa agaaaggaaa atggtcggag caatttgact tagatctttt tgaaaatctc
     1621 tttgtcggcc agaaatgatg attagatctg gctgaagctg attaattttt tctaagtctg
     1681 gttcttttat tccacctgct gattctactt ttttatagga tgaaagatac tcaggaaggc
     1741 tgcttgtagc tgctccaact accttatctc caacaccaag agcatccatc gtatctaaag
     1801 aaccattgtc gaatacgacg actttttctg gattatttgg cacctcgatt tggtctccat
     1861 tactatctgt tatcgtcaat gtagtttcag tttgtgtgct gctttttttt gtgtctgcct
     1921 gactggtgtt gttgctattt ccacatgctg ctaaaccaaa tgatgctaga atcaagatac
     1981 ctactactaa ttttttcatc tcttctctcc taatgttagt tttagaaata aagacagaat
     2041 ctgcggcctt caatctcaca aatccgtatg tccatttcgt ataattcatc caatacatct
     2101 ttttttatca tttcttgggt cgttccttgc gtaaataact gtccatcttt cattgcaaca
     2161 atttcgtctg cataacttgc agcaaagttg atatcatgaa gaacaataac aattgttttc
     2221 ccgatttctt gtactaaatt ttttaaaatc tgcatgattt gaaccgcaaa attcatatct
     2281 agattgttca aaggttcatc cagtaaaata taatccgtgt cttgcgctag caccattgca
     2341 atatacgcac gttggagttg accgcctgaa agagtgtcaa tcgattgatc cgctatttct
     2401 tctaacccaa tgtgttccag tgattcctga acgatttcct cgtcttttgt cgtcaatttt
     2461 cctttcgtat aaggaaatcg tccaaattct actaattctt tcacggtgat ttggacattt
     2521 atttcattcg attgtcgtag gatcgataat tttttggcta attcattgga tttccaagcc
     2581 tttacttcgt tatggtctaa atagatttca cctgtatctt ttggtatcaa acgactgatc
     2641 atcgacaaca ccgttgactt tccagcacca tttggaccga taaaagcagt caacttcttt
     2701 tcaggaatcg ttaactgtac atctttaacc actgattttc catggtacag tttattgacc
     2761 ttttgtaaat caatcatagg atactcttcc tttcgcgaat caattttgtg acaaaataaa
     2821 ttcctccgac aaattcaacg acgacactca ctgttgtatt ccagccgaaa atctgctcta
     2881 ctgcgagctg tcctcctgtc agaaacagca tagctaccag aaagcttccg ataaatagct
     2941 gaccatgacg ataagtagaa aagacacgat aactgatatt tgcgacaata aaccctaaaa
     3001 aagtaactgg acccacaaga gccgttgatg tccctgtcaa aagactgaca caacataaaa
     3061 tcaaaaattc aaaacgatct gtgtctatcc cgaggtttac tgcttgttca tttccgagat
     3121 ggaaaacgtc tagcacatgt cgatatcgaa ataacaggta ccagcagccg ataatgatca
     3181 aagaagcagg caaaagatac tgactctgga tattgccaaa gcttgcaaat aatctgcctt
     3241 gaagcaaatc atattcattc ggatccatga tgacttgtaa aaaggtactg acactaccaa
     3301 aaagcgtacc taaaatcatc cctgacatca gtaataaaaa aagattcgct ctcatttttt
     3361 tcaaaagaaa gaaaaataac aatagactca gcccgaccat cagcccaacg ttcaataaga
     3421 aaaaaactgt acctgtctgc tgaagcatcg tcactcctcc gataaagaag aaaaggaccg
     3481 tttggacaag gacatataac gactcaagtc ccaatactga aggagtcaaa aactggttat
     3541 gcgtaattgt ctgaaagctg attgtggcac tgctgacagc tagcgagaca aggataaatg
     3601 caaaaatttt cttactcctc aatgcacatg caaaatgcca gtttccatat gtatggatcg
     3661 tcaagtacat tcctgtaaca aataaaacaa gaacaagcat accaataaaa atagatggct
     3721 tttttttaag catatcgtcg tccccttatc agaagataaa ggaagatagc cccaccgatg
     3781 attccaacga tcacactgac tggtatttca taaggaaaaa tcaacacgcg agcgacaaga
     3841 tcgcaaaata ctaagaacaa actacctgct aaagcagtta cgccaattgt tttgtgcact
     3901 tgatctccat atattctaga gacaagattc ggaatgatca ctccgataaa aggaatgctt
     3961 cctaccatga tcaacatgac tgcactagcc aatgatacta agccaatgcc taaaaactgc
     4021 atcttctggt aatctattcc cagatttact gcaaacgatt cgcccagacc aatgatcgta
     4081 aatcggtaag caaatagcca aaccaagagc aacaatggaa caacagcata caataactca
     4141 taatttcctc gtatcacagt cgcaaaattc ccttgaagcc atgaagtcat attttgtacc
     4201 aactggaatt gataagcaaa aaaagtagca actgaaccaa tgatattccc taacatgact
     4261 ccaatcaacg gaagcatcaa ttgatttttc tggaggaaca atcgcgtaag ggataaaaaa
     4321 agcaatgttc ctgcaaaagc aaagcaaaat gctgtcaagg atcggacaat caaggatgca
     4381 ccaggtagga aaatcatagc cactaaaata cctaaacgcg cactatccat cgtgcccgca
     4441 gtcgttggtg aaacaaattt attttgggtc aaatgttgca tgatcaaccc acaaatacta
     4501 ctggtcgctc ccacaaggat caaacaaatc gttcgtggca aacgagtcac tagcaagatt
     4561 tgtaagcttt gcccatctaa ctggagcagt tctgacaatt gtacattcga tactccgatg
     4621 aacaatgaaa tactacttaa aataactaat aatgctacta gtcgtccgta gcgcatccaa
     4681 tccacctttc taattgagaa tgattttcat tcttatttta agtgaatcgt tgaacaaaag
     4741 caaccgcttt ttcatatgtt ttaggtatta ttgggaatat gctataatcg taagaaagac
     4801 aaagagaaga aggagggtat tttttatggc acaagatgat gtaaaaagcc atttggataa
     4861 gggactttat ggtacacctt tagtgaaccc tgaagaacag cataaatatt tagggacatt
     4921 cagggaacgc tgctatctta gtatgactgt tgctgaaatg atccaaccaa aaaacaaaga
     4981 aaacttttta aaagaactca aaaaacatcc agatgcttcc atactcttga atggggctct
     5041 acccctttct gtccaaaacc aatatatcca gctagcaacg aaaaacaata gccgtttcac
     5101 tgtagtcaac gattacgtca ctgcaaatcc tgaggaattc ggattgctct taacagcaaa
     5161 agaagcagta aatgaacctg tgattgatat cgaaaaaaaa tacccagaag aaaactcttc
     5221 gccagctgaa aaagaaaaac caaaaaagaa aaaaagcttc tgggatcgtt tattttaaaa
     5281 cgaaaaaaca cataaatttt tacatgcaaa aaaagctttt gatcagattg acatcaaata
     5341 ggacaatctg tcaaaagctt tttcttttaa aatacttcag gatataacaa tttcaataca
     5401 ttcatcgtca atcttctgcc ttttcctgca aaaggataaa catgcatatg catggctgga
     5461 ttgaaatttg cttcgatgat cccatatgct tttttatcag ttgtaggatc gatttcttta
     5521 tctggtataa tcaaatcgat accactaatc ttagctccta gtgcttctac cgcagaaacg
     5581 gcgatttttt tgtaagcttc agaaaattca tcagtcatat cgatcgaatc accacctgta
     5641 ctgatatttg agttctccct tagataaaca atttgattgg cttggggaac tgattcgatc
     5701 gtcaaccctt gttctttcaa catcaattgt tctaattcac ctaattggat caattctaaa
     5761 ggtgcacgat gattcgttcc acgcaaaggg tcactatttt tttcttcaac aagttctttc
     5821 actgttcgga tactgtcacc aataacatta gcagggactc gcaacatgat ggcttggact
     5881 tctccgtcga tcacaaagaa acgatattcc gttcccggca taaattcttc tactaaaacc
     5941 gaactgtctt cacgaaaagc aatggctaaa cctgcttgat aatcttccag actagctcct
     6001 tccttgaaaa tcgtgatacc taacccgtaa ttggttgatt taggtttgat cacaaatgcc
     6061 tgctcggcaa atctcggata agcttttacc gcttcttcca tggatgaaaa ttctgcgcct
     6121 cctggtaccc ggaacccagc ttcttttaag acttttttcg ttactgtttt gttttccatg
     6181 ataagtggga cgatatagct gtccttactt gtcatgtttg catttttcac atattctaca
     6241 tggtcttgga attgcagacg caaaaattga tccgactcat ctaatacttt gacttggaca
     6301 cctttttgga tggcatcgaa cataaatatc tgagtagaaa gttccatttc gcgaaaaccg
     6361 gccaactgat agggacgttc atggctttct ttataatatt gatgtcccaa tgccacagca
     6421 acttcttgct gactcgatag ctgtgcttct tgatagagtt tcgctgctaa cgtttgttca
     6481 ggatgatcca tttgagtcaa tgcttcctct actaaataaa gagaatccaa ccaatccaat
     6541 tcaaccagca tttgcttcat tccttcaaga atttctctac cttcttgatg aaattctgtt
     6601 tgatctgatg gttgttctag tgctactttg ttatttcgag tcgtaccttc tgctacccat
     6661 tggtcacagt cttctttttc ttctgtccat aacatgtaca tcaggaataa ttgtaagaac
     6721 cgaacttgct ctggattgat acccaatctt gcataaggat tcaaatcgat attccgcaat
     6781 tcaatataac gaacacctgt tgttgctaaa tctgcgactt tctttccgcc tcggaaacga
     6841 agcggtgtat aaaattcttt ctcctccgaa agtttccctt cttcgatcat tcgctcgata
     6901 tctgctaaat actgtttcat agaagcatag gaaactttca catttggatg attcgtatag
     6961 cccagcgcac tgttgcgaat actgcgaaca ggttcttgcg gatgtgattc atctaaaaag
     7021 tagttcttct cactcattgg tgaagcaccg aataaataag tgatcatcca gcgatagcgc
     7081 ataaagtttc tcgctgtttt caaataaagt tctgttttga attcggaaaa atcttgaaat
     7141 tcttcctgat ggctgaacaa tgttcttagc aactcatcgc caaattcaaa attgaaatga
     7201 ataccgctta ccatctgttt tcgcttcccg tattcttttg ctaaataacg tcgatacaag
     7261 acatcttcga aattttttaa ttttgcaata atgatctctt cgtctttttc cggtaaggca
     7321 ggtggcatac ttaatggcca gatcatctct tctttcggta tagaacgagc agtcacatca
     7381 taaacagcag cgagatactg aaataattcg ggaatagaat cggtaacagg ggtgatcatc
     7441 tctgtttgtg tttcgctaaa atctgtttgg atataggggt gataggatcg atcgccaaag
     7501 attgctggat gatcggtttt tgctagattt cctgcaagat caacccgctg tccttcccgt
     7561 tctataccaa atcgagcttg gtcgataaaa gggcgcgcat tgacatgcaa taataattgc
     7621 ttaaaattca tcattaagtg acctccaatt catatgaatc taattataat caaaaagtct
     7681 aagaaaaaga acgaaattgt ttgagaaccg cttacaaata agtataaaaa tgaaggcatc
     7741 aaaaacacag ctatgttaaa aacaatcatt caaacaagga gctgcaactt ttgtcacagc
     7801 tcctgctttt tagacatggg ttattcttct tttagtaatc ctttgaacaa tccaacgaca
     7861 aaatcatttg gatcaaacgg ctccaagtca tcgattcctt caccaagacc gaccaatttc
     7921 actggtaaat gcaattcatt acggatagca agtacgatac cgccctttgc tgtaccatct
     7981 aattttgtca aaacaagacc ggtcacatct gttgtttctt tgaattgttt tgcttgaacc
     8041 atcgcgtttt gaccagtggt tgcatctaat acaagaagtg tttcttgggg tgcttctggt
     8101 aattcccgct ggattactcg ttttattttt tctaattcgt tcatcaagtt cactttattt
     8161 tgcagtcggc ctgcagtatc aactagtaaa acatctgcct gctctgtctt tgctctttcc
     8221 aaagcatcga atacgacagc tgctggatcg cctcctgcat tgccacgaac aacttcaaca
     8281 ccagcgcgtt cgccccagac aactaactgg tcgattgctc cagcgcggaa ggtatctgct
     8341 gctgccatca atactttttt accttcgttt ttgaactgat gggcaagttt accaatactt
     8401 gttgttttcc ctacaccatt aacaccgaca aacaaaataa ctgttagtcc gtcttcttgg
     8461 atatttagtt catttacttc attgattcct tccgcttcat agagatccac aagtttctcg
     8521 atgatcgcat tttggacttc tgccgatttt ttcgcatttc tgagtttcac ttcttgacgt
     8581 aatgcttcgg tgattcgtat cgctgtttca aaacctacat ctgcaccgat aagtgtttct
     8641 tctaattctt caaaaaagtc ttcatcgact gttcgaaaat tggcaaacaa ctcattcatc
     8701 cgttcaccaa atgttttacg cgtcttttct aaacccttgt cgtacttctc ctgtacttca
     8761 acttcttctg ttttctcttc gccagtaaaa gcacgcttga ttttatcaaa aaatcccatt
     8821 tgactccccc tactctaata caaatttttg gatcactttg gctacgccat tttcttcatt
     8881 gctggctgtc acgaaatctg ctgcttcttt gacgaaaggc acagcattct ccatcgctac
     8941 gcctagtcca gcatattgga tcatcggtaa atcattttcc tcatcaccga tcgccatgat
     9001 ttcttcttgt ttcaaaccaa gatcttttgc caataaagaa ataccataag cttttgtaac
     9061 accttttggc ataaattcca aaagcatact tcttgttttg atgatctcat acatttcatg
     9121 gaattctgct ggtatttctt tgattttagg atctaattct tcttggtcat aagcgattac
     9181 tgccttatta agcacaaatt catctgtaat atcgcttata tccgctggtt ggaattgcag
     9241 cagcttattc aacacattgt acaaggattg cttccttggg gcactaggca gctgtaaaac
     9301 cacattgtca gacaaaacat ctagtggcag atccaattgc tgagccagtt tgtataagcg
     9361 ttggatatct gatacttcca tcactttctt ttccatgatt tcaccagtat catttttttg
     9421 taccaaaccg ccgttgaaag tgatgctgta gtcaccttca tctaccagtc ctaattcttg
     9481 cagataatgc gccatcgcag ctaaggggcg tcctgtacat agaacgatct tgaccccttg
     9541 ctgttttgct tgtgctaatg cttgtttatt ctcatctgaa atcagctttt gttcatttaa
     9601 taatgtaccg tccaaatcga ttgctactaa tttgatcatt ttcgttccta cactttcgtt
     9661 tcgttgattt tcgcctcttc attgatatct tccatccgta cagaaacgat ttttgataca
     9721 ccggattcct gcatcgtcac tccgtacagt acatctgctg cgaccatcgt tcctttgcgg
     9781 tgtgtgacta cgataaattg tgtgtcatct tggaaatcgc tcaagtaacg accaaaacgt
     9841 ttcacgttgg cttcatctaa cgctgcttct acctcatcta agatacaaaa cggaacagga
     9901 cagacacgga taatggaaaa gagcaacgcg atggctgtaa gtgctcgttc tcctccagaa
     9961 agcaaactta gactttgcag ttttttgcca ggaggttgta cttcaatctc aatacctgtt
    10021 ttcagcaaat cagttggttc cgtcagtact aattcagctt taccgccgcc aaacatattt
    10081 ggaaagacaa ttttgaattc ttgacggata gcttcaaata cttctttgaa tcttgtccgc
    10141 acttcatcat ccatctcatc catcgtttca aacaattggt ttttcgctgc taacagatca
    10201 tctcgctgtg ttgctaagaa tgtatgtctt tcactgactt gttcgtattg ttcgattgaa
    10261 tttaagttta ctggtcccag tttttcaatc tgttctttta aggatgaaac ctttgttcta
    10321 ctgtcttcga tatcggttgt ttcttgatag tcttgggaag ctttctcaaa tgtcagctga
    10381 tattctgact gcagataggt caaatgatta tctaaaatca tttcagctcg gtctttttgg
    10441 acttctaatt tggattgttc cgacagtcgt gctttttgct cacgattacg ctctgccaag
    10501 actgcttcaa gcttatcgat ctccttttgc cttctatctc gctgctcttt ggcttttgcc
    10561 aactcggctt ttaatgtttc tcgctgagca gataattcgt taatttgttt ttccaaactt
    10621 tcttctgtga cttcatgatc agaaaagtct gctgtcaatg tcgccaactg cttttctatc
    10681 gcttcttgac gttcggttgc ctctgctttt tggactcggg caccgcggag ctggatctgc
    10741 agatgattaa actgctcttt caagacagca agatctgctt gttcttgtgc tttttgactt
    10801 tgtacttgtg ctcttctggc ttccatctgg tcgctttctt gacttaacga tttgatttct
    10861 tcgtcgattt tttgtcgttg cgattcaaga tctgtttgtt tgtctttcaa ttcgttcgtt
    10921 tgcttttcgt aatcttcgat aaactgctgc agttcacgtg tctcaaaagt tgagatttgc
    10981 ttttcttttt caaaacgttc aagttcattt atgatgtttt gtagctgatt tgtcgcttct
    11041 tgttcttcaa aacgtaattg ttcgcctcgt gtacgcaaaa cttcttgttc ttcggctaaa
    11101 cgagctgttt cttgttgcag ctcctgaact tttttttctt gtgcttggag ttgtttatct
    11161 gcttcttcaa attcactagt caactgtttc aattcttgat tttgtacaaa caaactgcct
    11221 tggtttcctc gtttattcgc tccacctgtc atggatcctc cggcattcat cacatcacct
    11281 tctaaggaaa ccactcgata ttgatagcgg atcgtttgcg cgattgcatt ggcactagtt
    11341 aagtctttgg ctaaaaggat cgttcctagc aggttttgaa ccacagtttg gatttgatcg
    11401 ggataactga cttgttcact ggcaatccct ataaaccctt ctacagctgc tgcttgtgtc
    11461 aatatatgag ccggtaattg tcttggtttg attgtcgtca gtggaaggaa tgttgctctt
    11521 cctcctcttt gctgtttcaa atacgtgatg gcttgccgag catctttttc attttcaacg
    11581 atgacatgct gggctgcccc acctaaagct gcttcgatag ctaacgtgaa atctgcagga
    11641 acatcgatca gttcagccac tgctccgaca atacccgaaa gctgctgttt atgctgcaag
    11701 accaatcgaa ccccttggta aaaaccaaaa tagttttctt ggatttcttg aaggcttttc
    11761 ttacgagcac gtagctgctg gacttgattc attaattgat acattttagg ctgttctttt
    11821 gctaatgctt cttggaactt tttcccgttt tgttggatca tttctaactt ctcttttgtt
    11881 tcagttaaag atgattttag ctgcgcctgc ttttgtgtca attcttcttt ttgtaagatc
    11941 aatcggtcta ctgatgcttc tacttccgat tgcttggcta acgtctgctt gctttttgct
    12001 gtttcttgta tatattgacg ttctagatac ttcagttcgt ttcctacagc tgcttgttct
    12061 tgcatcaaat caacatattg atctcttaat tctgccaata attctttcgt tgatttttgg
    12121 tatttttcta attcttgctg tgtttttatg agatttgctt cagctttttg gatctctgtt
    12181 tctttttctg ctgctgcttt catcaagctt tcttgaagct tttcaaaatg tttaaccttt
    12241 ttttgtgctt ctgctaatga agtttgatat tcttgcgagc ttttttgtgt atgctttgtc
    12301 cgctcctgca aaacatcttt ttgtccttct gtctgcttca gtttttccga aagatctaat
    12361 aatacttgct gatttttttc gatcagacga tcggcttgcg cattttcttt acgctgcttc
    12421 gccaaaatgg attcttgttc ttggatcgac tcagatagtt tgcctaattc tagattgaat
    12481 ttcgcaagct gtgcttgttt gttgtcccaa tctttttttg ctgtcttgat ttcagcaacc
    12541 atcaaagaga catcggtttg agtcagtgtt tcttttagcc gtaaaaactc ctttgctgcc
    12601 tcactctggg cggccaaagg agttaactgt tcttccaatt cgtggatgat gtcttggacg
    12661 cggctgaggt tgtcttctgt ttcaaaaagc ttctgttctg ctttcttttt ccgctgcttg
    12721 tatttcaaga caccagctgc ttcttcaaag atcccacgac ggtcttccgg tttactactg
    12781 aaaatcgctt ctacttttcc ttgagaaatg attgagaacg attcttttcc taatccagaa
    12841 tctaagaaca attcttggat atcttttaat ctgcagcttt gtttattgat gaaaaactcg
    12901 ctttccccag tccgtcgata acggcgggtt acactgatct cattgtactc caatggcaga
    12961 taatgatcac tattgtctaa aatcactgtg acttctgcaa tattcagctg cttacgggta
    13021 tcggaaccgg caaagatgat atccggcatt tttccgcctc gcagactttt agcagattgt
    13081 tctcctaata cccagcggat agcctctgta atattgcttt ttccgctgcc atttggtcca
    13141 accactgctg tgacgctatt ttcaaaatca atgactgtct tgtctgcaaa tgatttaaaa
    13201 ccggctattt caattcgttt caaatacaaa gctttccctc acttactttt acagtgctgc
    13261 caatgcattt tccgccgcag cttgttctgc taattttttc gatttccctt gtccagtgcc
    13321 aatcaattga tcatctacat aaacttcgat ccaaaatact cgttcatgag ctggaccttc
    13381 ttcattgatc aatcggtatt cgattgatac atcacctgat ttttgcaaga cttcttgtag
    13441 ttttgtttta tgatccatct catgtgaaaa agcacctgct ttgactttag gaaaaatcac
    13501 tttttcgatg aatgcatggg tcgtgtcgaa tccctggtca agatataacg cgcctaaaaa
    13561 agcttcgaat aggtcgcaaa gcaatgctgg tcgcgtacgt cctcctgaat tctcttctcc
    13621 cttgcccaac aaaacatatt gatcaaagtg acattctttg gcaaatttcg acaagctgtc
    13681 ttcgcgaacg atcgcagctc gtgtttttgt caattttcct tctggcaaat gaggatataa
    13741 acggaataaa tactgagaaa ccatcagttc taaaacggca tctcctaaaa attcaagtcg
    13801 ctcattatcc gataattgca gattgcgatg ctcattcaca taggatgaat gggtaaaagc
    13861 ttgttcaagc aaatttagat cgtgaaaaac gatgttatag ttttctttta gttcttttgt
    13921 cagctggttg tccattttcc acattccttt tatggatatt ttttcgatac ttccttatta
    13981 tactggtttt ttgtcaaatt tgaaatggtt cggtcaaaag gtaagtaaag ctttaataat
    14041 cctagtgatt gacgagaagt tgtgaaaaat tcgtttagtt tcgagtatga agaaaaaatt
    14101 ttgtgaaaat ggctttccat atttttgcat aaatttgact taataccgag aaactggact
    14161 ttgaacaccg tttattggag aagtcatgaa atcagcgcct tgacctgaat attagagaaa
    14221 taaacagaaa aatatcaaaa aaccgaaaaa ggacatcgcc ctttttcggt catttttagt
    14281 gattatgaac gtgaatctgc tgtaacagaa acatttgccc atgggtttgg tacatcataa
    14341 gaccaatccc agtctttgac acggtcgttg actggcaata cttcattacg gaacaacgtt
    14401 ggaatcgcaa atgcttcttc aaatgcatat tgttgccatt tttgatatgc ttctttttgg
    14461 tactcttcat caaatgcttt gcttgaactg atatcgttga tcaattttgt attttcttct
    14521 gattcataac gagtgtagtt gaacgcagca tttggtcccc atagtccact tggatttgga
    14581 tcagttcctg tattccaagc agcttggtaa acatcaattt caggatcatc gttttctaat
    14641 ttatcataga aagaattgaa ttcgatcaga cgacctgttg tgtattggac atccaaacca
    14701 atttctttcc attgttggat atagtaatct gccaatggtt gcgctgtttc cccaccggac
    14761 atcgaagcaa agttgattgt tagtttattt ccatctttgt cttctcttaa accatcgtca
    14821 tccacatctt tgtagccagc atcatctaat agttttttcg ctttgtctag atcttgtgtg
    14881 aatcctttca tctcatcatc atggtatgac tggaatactg gaggaatcaa tgaagtcgca
    14941 ttgcttctca aacctgcata gaaacgttca cctactgcat tattatctaa tgcatagccc
    15001 attgcttgac gtaatgattt atcagccatt tttgaatctg gattgtattc cacactcttt
    15061 gtatctgcat tccatttacc taacttgaat cctagataag tataagaagt ttgttcacgg
    15121 cctaagtttg tatagccagc aatttcttta taggtatcgt aagtatctgt tggcatttgg
    15181 aaaatcatat catataattt agagttcaat gcttcagttg cacttgccat cggtacagat
    15241 ttcatcgtga tctttttcaa tttaggcgta ccattccagt agtgctcgtt tggcaagtat
    15301 tctactgatt ctcctgcaac gatattactc atgtaataag ggccaaaagt cactggattt
    15361 ttacgtactt gatcgcttga ttccatatct ttgattgcta caccttctaa cgtatgtttt
    15421 ggtgctgcat atgaccagat accaccaccc gattgaagca tgcttgggtt tacttcttta
    15481 tattcgattt caactgtttt gtcgtctatt tttttgattc cggagattgt gtcggcactt
    15541 ccatcatgat attcttccat acctacgatg ttacggaaag tgtcgtcata acggatacca
    15601 gtgtaatctt tattcccaat gatttcgtaa gggaaaatca catcgtccgc cgttacatct
    15661 tcaccatctg accatttgac gttgtctttt actttgattg tcgctttttt cgtatcttca
    15721 tctagatcaa gcgttgcagc tccgtcgtct gtgattctga aatcgtcatc gtttgcaaat
    15781 agcggttcat gagaaggagc cataaaagct gcatcatatg aatcttggta aaactcccac
    15841 tggaacaatc ctttaaactg actgtccgtt gcgaccgcag cttctaattc gccgtcttcg
    15901 attgcatcat cattattttt tgttgcaatt ggaaaagtta attcctcttt gttattgccg
    15961 ttgcctccag agttgtttcc agaggtatct gtacctccgc ccccgcatgc tgcaagaacg
    16021 acagcagaca tcagcgctgc aaatccaaaa atcgttttct tcattcctat ttcccccttc
    16081 gcttctatac tagcctaacc gttggcgcgc atctgcagaa cgcttgaatg cttgtccaac
    16141 ataattgata ctcaacatca aaactaaaat aaggattgat gcgggtaacc agatccattg
    16201 ccgattcact aagacttctc cgttacttgc ataaccgatc aatgtaccta aactaggtac
    16261 atttgccggc aagccgaaac ctaagaaagt caaagttgtc tcgataccga tgtttgccgc
    16321 aaagttcatc gtcaaattag taatgattaa tgaacttaga ttcggcatga tttcgcggaa
    16381 catgatttta aaatcattgg ttcccattgt ttttgaggca ctgacataat ctcttcttgc
    16441 ttcagataat gtcttgcttc ggaataatcg ggctttagct acccaataaa atgcactcat
    16501 gatgaatata aaggaccaga cattgtatct ggaaataatc gatacgaaga cgataatgat
    16561 cagcatgatc ggtagaatca tgataaaatc aacgatccgc atcaagatgt tgtcaatgat
    16621 ccctccgtag tagccagaga tgatccctaa tccaaccccg atgatcgaag taaggatcgt
    16681 gatcgcaaat ccgatcgtaa cagagtttct agcacctatg atcaattgtc ctaatacatc
    16741 acgtccgcct tcatctgctc ccagaatgaa cttttgtcct tgtgtcgaaa ctgttcctgg
    16801 tgcagcgtat ttgtcgaaga tactaacata catcactttg tcttgatcgg tcagcatcgc
    16861 accgataaag atcactaata ataaaaccac tagcaaacct aatgaaaaaa tagcaatttt
    16921 atctttttta aattctcgag caatcattcg tatccccata ggaggaatac tttcttgaac
    16981 tgctgtattg tttttttcac tcatcgatcc attctccttt ctgattggtt ttattgaatc
    17041 cgaatacgcg ggtcaacgat actcatgatg atatctgaaa gtaatgtccc cactagcgtc
    17101 gcgattccta aaatcaatac taaggatgtg atcacggtat agtcccgttg gccgatggaa
    17161 tcaataaata gttttccgac tcccggataa ccaaaaatct tttcgataac tacagaacca
    17221 gcaatcaacg aagtgatttc ataccccatt tgagaagcca tcggcaacgc tgcatttcgg
    17281 aaaatatgtc gactgaatac tttattcgtc ggtacccctt tggaacgggc tgttctgaca
    17341 aaatcgagcg atttcgaatc gattacttca cttcgcagat attggattgt gacggcagtc
    17401 gccaaaaatg cttgggtaat tgatggtaac agaatatatt tcaaacggtc tatccattga
    17461 gtgataaatc caccttgcaa acctagtgtt tgtgatccac ttgttgggaa ccagcctaaa
    17521 cgataaccaa aaacaaatag catgatcaag ccgaagataa ataaaggaac agcaaaacta
    17581 aagaagttgt agattacgac aatcttatcc aagattgaat tttgataccg gccagcaagc
    17641 atccccagag gcacagcaat caaataagta aggacaactg tcaagatcga tagatataaa
    17701 gtgttgatga tccgttcgcc gatcaagtca gcaacagagc gtttgtataa gaaactttga
    17761 ccaaaatcgc cttgcaaagc atttccgatc caacggaagt actggacata ccatgggtca
    17821 ttcaagccag ctgcttgtcg catctgttcg atcactgccg gatcctgatt tggattgatc
    17881 aaaccggtaa atggatcccc cggcatcgct tgggctaata agaagatcag aacacttaaa
    17941 ataataactt gcgggatcat caataaaaca cgtcgcagaa tcgttttcca cattagcttg
    18001 cacctccatc tttcaacgcc actttgtgtg tagttgtgat cgtacgcaaa tcgtagactc
    18061 gaccagtcgc atcatagtat tctttttggt tttggatata ctcttgttct actcttcgtc
    18121 gattttcttt gtggctttcc ctattttcta catcgatttg cggaatagct gaaagcagac
    18181 gtttcgtata gatatgtcgc gggtcattat aaatgtcttc tcttgtaccg atttctacaa
    18241 atcgaccttt gtacataatt gcgatattgt cgcacatgtg ttttactaca cctaaatcat
    18301 gtgaaatgaa taaatagctg aggccgtatt cttgctggat tcgtttcatg aaattcaata
    18361 cttgcgcttg aacagataga tccagagcag atacaggttc atcggctacg atcagttttg
    18421 gattggtagc aacagcacgg gctactccta gacgttgacg ttgtccgcct gaaaattcat
    18481 gaggatattt atatagcgca tcttccggca tcccaacgat atctagcagt ccttttacct
    18541 ttttcttttc ttcttgatcg cttaaccgtt caaaatttcg gatgggttca gcaatgatat
    18601 ccaacactcg ctttttagga ttcaaactgg acattgaatc ttggaagatc atctgcacat
    18661 ccttgttata acccattttt ttgcggttgc ttcgcttcgt tacatctttt ccttcataca
    18721 tgatttgtcc ggctgttacc ttttctaatc ccacaacggc ttttcccgta gtagattttc
    18781 cagagccaga ctctcccact aaaccatatg tctttccttt ttcgataata aaatccacac
    18841 catctacagc atatacatga tccgttactc gattgaagaa tccactgcga atagggtaat
    18901 gaactttcag gtctttgatt gtgattaatt ctgtcatcta cacctctcct ttttcgtctc
    18961 taaagtggaa atgcttatag caggagcaac gtaccaagtg gttcggtgcg acttcatgaa
    19021 ggataggctc ttcttcatga tcgcttgctg gtatccatgg aatacgtggt gcaaaccgac
    19081 aacctttacg aggcatgttt ttcagagaag gaacgacacc ttcgatcaca tggagttctg
    19141 catcatgatt tccttcttgt ggtatagagt tcagtaaaga gcgtgtataa ggatgcttag
    19201 gatgatcaaa cagttctttt acacctgcta cttcaacgaa ctgtccgcca tacatcactg
    19261 ccactcgatc agccatttct gctacgactc ctaagtcatg agtgatcaag atgatccctg
    19321 tttttgtttc ttcctgaagg tctttcagta aatcaagaat ctgtgcttgg atggttacat
    19381 ctagggctgt cgtcggttca tctgcaatga cgattggagg tttgcaggcg attgcgattg
    19441 cgatgatgac acgttgtctc ataccgcctg atagttcatg gggatattgg cgtccaactc
    19501 tcgcaggatt aggaatccct acttgttcta gcaattctag tgcacgagct tgacgctgtt
    19561 ctgcattcat ctttgtgtga taggaaagtc cttctttgat ctggtcttcg attctcatga
    19621 gaggatttaa tgcagacaac ggatcttgga aaatcattcc gatatcattt ccgcggattt
    19681 tattatagag cgtttcgttc aggtcgatta aattcagatc gttatataaa atctcacctg
    19741 taatttttgt gttattagga tcaagtaatc ccataatcgt agtagccaag gtacttttcc
    19801 cgcacccaga ttccccaacg attgctaaaa tctcgttttt gtctaaagta aatgaaacgt
    19861 catctactgc atcgtaatag tcatctttga gtcggaatcc tgtatgtagg ttctgaactt
    19921 ctaataattg gttatcggac aatcgaatac ccctctctgc ttcctaaatg gaagtttttc
    19981 ttaataaaaa aggacattac acagaataaa tcgttttata acaaaggtta ttcttatctt
    20041 tgtgtaatga attattaata attatataga aaaccgcttt actttgcaac ataatttctg
    20101 tttatcgcca gaattatcag aaaaatgact tttacgtgtc aaaaagatta aaaaaagaag
    20161 gataaaaagc tgcatgcagc ttttt
//
LOCUS       DAFFSV010000043        19532 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFFSV010000043 DAFFSV010000000
VERSION     DAFFSV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.043"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..334)
                     /locus_tag="LR110_001393"
     CDS             complement(<1..334)
                     /locus_tag="LR110_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL4822361.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSS"
     gene            complement(421..1125)
                     /locus_tag="LR110_001394"
     CDS             complement(421..1125)
                     /locus_tag="LR110_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBL4822362.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            1325..1591
                     /locus_tag="LR110_001395"
     CDS             1325..1591
                     /locus_tag="LR110_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4822363.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            1604..1762
                     /locus_tag="LR110_001396"
     CDS             1604..1762
                     /locus_tag="LR110_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822364.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(1759..1989)
                     /locus_tag="LR110_001397"
     CDS             complement(1759..1989)
                     /locus_tag="LR110_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL4822365.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            2061..2216
                     /locus_tag="LR110_001398"
     CDS             2061..2216
                     /locus_tag="LR110_001398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822366.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            2373..2528
                     /locus_tag="LR110_001399"
     CDS             2373..2528
                     /locus_tag="LR110_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822367.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2525..2749)
                     /locus_tag="LR110_001400"
     CDS             complement(2525..2749)
                     /locus_tag="LR110_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL4822368.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3016..3351
                     /locus_tag="LR110_001401"
     CDS             3016..3351
                     /locus_tag="LR110_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822369.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3584..4525
                     /locus_tag="LR110_001402"
     CDS             3584..4525
                     /locus_tag="LR110_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBL4822370.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            4527..5417
                     /locus_tag="LR110_001403"
     CDS             4527..5417
                     /locus_tag="LR110_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBL4822371.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5460..6260
                     /locus_tag="LR110_001404"
     CDS             5460..6260
                     /locus_tag="LR110_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL4822372.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6275..7126
                     /locus_tag="LR110_001405"
     CDS             6275..7126
                     /locus_tag="LR110_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL4822373.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7123..7440
                     /locus_tag="LR110_001406"
     CDS             7123..7440
                     /locus_tag="LR110_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822374.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            7433..7615
                     /locus_tag="LR110_001407"
     CDS             7433..7615
                     /locus_tag="LR110_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822375.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            7617..8087
                     /locus_tag="LR110_001408"
     CDS             7617..8087
                     /locus_tag="LR110_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822376.1"
                     /translation="MAMIPKFRAWDREKDRMIYPSTTGVCFEMNDDGISVLDIDVDYP
                     NDYGFPKIASILMQSTTLKDKNGVEIFEGDIALITHKSTNYADTYWHSYVEVFRAKNG
                     AYRIRGEHIYETELYSNRKQLTVAGNVYNSPELLKRDRFMTTTENPELLKEEQR"
     gene            8084..8626
                     /locus_tag="LR110_001409"
     CDS             8084..8626
                     /locus_tag="LR110_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBL4822377.1"
                     /translation="MNKQELIDKYTAEISRLRPYCPNRHLISDQLKYGLYKEILEDLK
                     QLDEPQKPAVPKFVAEWIEYAKKKGDSLAISFKPWNLYGVEYSKTDRWIEDNQGTFAR
                     AWLIGYEVEKEPLYYVKLPVVYFNHWDLETYLMKDDRGNITIADNNDFDDMKFTESEI
                     KAIDERYWPFAVPVEEVAEG"
     gene            8623..9006
                     /locus_tag="LR110_001410"
     CDS             8623..9006
                     /locus_tag="LR110_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822378.1"
                     /translation="MSNLKVGDIVFYKGDEGIIREVMTDGKENFYRVDVSRKSILYLY
                     EDELDLGTQKLNDNQKMVFDYLKSNVEQGGKSVMYSIFLLGDWDSRIGAARNVDIAYC
                     GLNGKQEAEVLSAFAQWGLEQEEAE"
     gene            9003..9233
                     /locus_tag="LR110_001411"
     CDS             9003..9233
                     /locus_tag="LR110_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822379.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            9240..9443
                     /locus_tag="LR110_001412"
     CDS             9240..9443
                     /locus_tag="LR110_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBL4822380.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            9440..9736
                     /locus_tag="LR110_001413"
     CDS             9440..9736
                     /locus_tag="LR110_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822381.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDIELINDK"
     gene            9813..10226
                     /locus_tag="LR110_001414"
     CDS             9813..10226
                     /locus_tag="LR110_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL4822382.1"
                     /translation="MQLLREVAFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            10239..10625
                     /locus_tag="LR110_001415"
     CDS             10239..10625
                     /locus_tag="LR110_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822383.1"
                     /translation="MWKDYVSLKELKKDLIFKKIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHSSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKEKLCVVTDA"
     gene            11117..11189
                     /locus_tag="LR110_001416"
     tRNA            11117..11189
                     /locus_tag="LR110_001416"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11150..11152,aa:Lys,seq:ttt)
     gene            11575..11835
                     /locus_tag="LR110_001417"
     CDS             11575..11835
                     /locus_tag="LR110_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822384.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            11878..12051
                     /locus_tag="LR110_001418"
     CDS             11878..12051
                     /locus_tag="LR110_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822385.1"
                     /translation="MKQYTAKDFEEMKRLKKDYEEVDMELTVGVIQRRLRVGLETAKA
                     IYNDLNAIEEKNG"
     gene            12440..13273
                     /locus_tag="LR110_001419"
     CDS             12440..13273
                     /locus_tag="LR110_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HBL4822386.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            13266..14681
                     /locus_tag="LR110_001420"
     CDS             13266..14681
                     /locus_tag="LR110_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBL4822387.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            14693..16222
                     /locus_tag="LR110_001421"
     CDS             14693..16222
                     /locus_tag="LR110_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL4822388.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            16140..17192
                     /locus_tag="LR110_001422"
     CDS             16140..17192
                     /locus_tag="LR110_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBL4822389.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            17194..17511
                     /locus_tag="LR110_001423"
     CDS             17194..17511
                     /locus_tag="LR110_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822390.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            17638..18261
                     /locus_tag="LR110_001424"
     CDS             17638..18261
                     /locus_tag="LR110_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBL4822391.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            18276..19166
                     /locus_tag="LR110_001425"
     CDS             18276..19166
                     /locus_tag="LR110_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBL4822392.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            19189..19404
                     /locus_tag="LR110_001426"
     CDS             19189..19404
                     /locus_tag="LR110_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822393.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
BASE COUNT         7002 a         2977 c         4129 g         5424 t
ORIGIN      
        1 atgaagaagt ttcatctata ttcggaataa ttactgattt atagatagtg accagagatg
       61 tgtcatattt ttgattaatt tcatcgtcta accgctggct ttcagcatta agaatatcaa
      121 ttttgttttc tagcgttgac aagtttttat tcaatttact tatgtttttc tctttttcag
      181 atataaacgt ttccctagca tttaattctt gagaatattc ctttacctta ctctctaagg
      241 cagaagtttt atcttttaat tttttagctt cagataattt gctactaccc ataattatta
      301 aaactattcc aactaaaaac aatagtactc ccattcaaaa cgcctcgctt cttttataaa
      361 ataattgtca aaatcatata aacccttacc gaagttgacg gctccggcat gggaattttt
      421 ttaaaataca acttttccga taattctaat ctgttctggc gatacaattc tatcttcgta
      481 taagtcgttt atagaacgta aaatgattgt ttgattttcg taatctttat atatctcttt
      541 acatgtaact ccgtcaccac ctaattccac aatagcaatt tccccatctt ctacgtccgg
      601 ttgttcttta acgaatacga attgtccatt cctgataaca ggttccattg aatcgcctat
      661 aataggtact gcgaagtcag cagcacttgg aactctctca aattcttcct cttcgacaac
      721 cgtatcaccg taagtaagtt ctgttggatt ggcagccgaa gccgaattgt gaacagttgg
      781 gattttttct tttttaggaa aatctaggac attttcttgt ttttgttctt ctaatcgatt
      841 acttgcaaat tcataggtag caactttatt ttcgtcgtta agctgattat atataggaag
      901 gatattatat atctgattag atatagataa atcaatattc atcaaatcat ctatatcaac
      961 attgaatatc ttagctatct catttaaggt ttttaatttt ggtgtatatt ttcctttttc
     1021 ccattcgctt attgaggatg aactttttct tcctagtttt tcggctagtt cttgttgatc
     1081 aatattatac ttttctctaa gaaactttag attttcagcg aacatagtaa cacctccttg
     1141 agttaattgt aacaccgttt cggaaaaaat gtaacaggtt tcttagaaaa tattttagtg
     1201 tttttaaaga agtatttacg caaaatagaa atcgatttca ggaaaactga aatatgcact
     1261 tgacttcgga aaaaccgaag tatataattt agtcagaaag ttaaagagag gaggcgaata
     1321 aaatatggat accgcgaaga aagaaaaata tacattgaga caattaagag ctttaaaggg
     1381 ttattcaaaa gaagaactgt caaggaaaag caaagtgaca tcaagaacaa tatttattta
     1441 cgaaaacgat gtagataaaa tgaggaacgg aaagtacgcc actctagaaa aaattgctaa
     1501 ggcattgggt gttagagttt ccgatatttt tttagaccct gattcggaaa aaccgaaatc
     1561 aataacaggt gaaacaaaac aagtcagcta ggaggtgtga atagtggaag agaagaatat
     1621 aaaagtaccg actcttgaag aatatacaaa aattgttgtc gaaacagacg agcagaaccc
     1681 gaaaacaatc gcagttatta cggcgaatga tattgaatca tgcgaaggtt ttcgagttcg
     1741 aatgacccct agatatgatt aatgttcttt atcacgtgga ggtataggat cgtttccaaa
     1801 actatctttt gattgtattt gtccattctt tttttggaca ataaactctg attgctgttt
     1861 tttagaaatc tttttaccat actcaacagc atctgattta ttatcaaata gttttgtatc
     1921 ttcggaatta ccttcaccac gtacagccca tttgccattt ctaggagata cccattgatt
     1981 tttacccata acaattcacc tcgctttcaa gttaattcta ttaagaggtg aaaagaaaac
     2041 aaactgatgg aggtgagatt gtgagtaaca agccaaaaat cactttgaat ggcacaccta
     2101 atcttcaaag attagcagat gaaatggcta gaatacgttc tagacattgg ggagttactg
     2161 tggtggcaac cataattgaa caaaaagaaa agcccacaca gccggcaagc aagtagtggg
     2221 caccatagaa caataatatt atatcacaca ctcgccactt ccgaaaagag gagttgctgc
     2281 tctcctttca agtttgccaa agtccacgtt tcttcatttt ggcaactcct cttcttggag
     2341 gtggtgacgg cgagagacga aggagggatg acatgaagga agaatcaaaa atagggattt
     2401 tgaatgagaa caattacaca agattaatta ttgaaacaga agaaggggaa aaagtagcag
     2461 aagttacatt gaccgatgca actcctgctg acggttatcg tattcgatta actcctaaat
     2521 atgattaacc ttttggtggg tgtggatcgt ttccgtgact atcacggctt tgaatttttc
     2581 catctctgcc atgaataaat agctcagatc cttgattttt agaaatctct ctagcgattt
     2641 ttactgcttc tgattttgta ttagtatgtg cagtcgcttt agaatttcca gcacctttga
     2701 cattccaacc accattggaa ttaggtacaa catgttgatt ttttcccata gttttcacct
     2761 ccatttctat agaaatattt tatcaaatag aaatggagaa attcaataaa cgaaaggagg
     2821 gatcaaatga cattacaaga gcatcaacac ttgatgcgaa aactaaatca ggaataccac
     2881 gaggaaatga aacctagatt gctaggggaa caatggcgtg aacgccagaa gaagtggtta
     2941 gaaataaaaa agtgactcag ccgtccaaag caatgagtca caaaccaaaa attataccta
     3001 aggagatttt atcacatgaa taagaaaatt gaaaatctta tcgaagaatt gaaacgtgaa
     3061 tgccaaaagc aaggtgtttc tattatttgt acagcccaaa aagaagggga acttaaaagt
     3121 ctcgtttatg gtgagacaac agaaatttta ctttgtcttg ctatgcaaga agaacattta
     3181 gatgaaaatc tcccattgtc agcacatatt atgcgtagaa ttgctgtgga tgcttacgaa
     3241 caagcgaaaa atgaagaaga aaatcaacct tcaaatcata cttttgttat taataacaag
     3301 gaagatttag ctgatgtgat gactcgtatt ctcaaggggg aatttcaatg atgaatgaaa
     3361 aaacaattga tctactcatt attttatttt tcacgttagt gattgtagcg aaaagttatg
     3421 taatctttgg cttgttattc gcaattacca tgcttgtgct ttgcgtgtta gcaagcagaa
     3481 aagaggttaa acatgaacga gttagcaaat cttgacaact atttaactga tcctgattat
     3541 gctaaaccac catatgaagc gccaattgat gaggaggatg aagatgagta aatctacctt
     3601 agaaatgagc catcaagaat ggctcgaaga ccgcaaaaga ggtattggtg gctcagatgt
     3661 tgcaaccgta cttggattaa acaaatacaa atctccttat caattgtggc ttgagaaaac
     3721 gggtcaaatt gaattgaaag atttagaaag cgaaccagct tattggggta atgttttaga
     3781 agaagtggtt gctaaagagt ttcaggaacg tacaggcaaa aaagtacgca gaagaaacca
     3841 agtatttgaa catccattac atccattttt aagagcaaat attgatcggg aagtagtggg
     3901 agaaaatgcc attctggaat gtaaaacagc caatcaattt ctcggaaaag agtgggaagg
     3961 tgaagaggta ccgcttagct atctctgtca agttcagcat tacatgaacg ttttaaacaa
     4021 agactattgt tatatcgctg ttttgatcgg tggacaaaaa ttcatttgga agcggattga
     4081 acgagatcaa gagctgatcg atacaatcac tgaacaatta gtagagtttt gggaaacgaa
     4141 cgttcttgga ggtatcgagc ctgtaattga cggaagtgga gcgactgctg acttcttaaa
     4201 agaaaaatat gcagatgtag aagaaaatca aacagctcta ccatcacgtt ttgatgaact
     4261 tatcgagcaa aaaagagaac tcaagcggac gaaaaaagaa attgaatcat ctattcgtca
     4321 agtagacaac gagatcatca gtgagctagg taaacgtgaa gccagtatcg gtatcacaca
     4381 aagaaacatt atcagctgga aacttgttcg tacaagacgg atgaacacga agaaactagc
     4441 agagaaatat ccagatgtcg caaatgatga agagatttat aacgttactg aatcaagaag
     4501 gctaaccgaa aaggagatca aataatatgg caacaaatga atcgttaaaa aatcaattgg
     4561 cagaaaagcc acagaaacaa gttgcaccag gacagttagg gcttaaagct ctaatgaata
     4621 caccaacaat gagaaagaaa tttgaagaag tacttcatga caatgctaat gcttttatgt
     4681 cgaatgttat gactcttgta tctaatgaca gttatcttgc agatagtgaa ccgatgtcta
     4741 tcatgagtgg tgcgttaact gctgcaacat taaatcttgg gctagataag aatttaggtt
     4801 atgcatattt agttccattc aatagtaaaa acaagcaaac aggaaaatgg gaaaagaaag
     4861 ctcaattcat gcttggctat aaaggatata tccaattagc ccaacgatca ggtaaataca
     4921 aagcattaaa tgtgatcgaa gtttacgaag gagaactaaa aagctggaac cgactgacag
     4981 aagagtttga gtttgatcca aatggtagaa cgtctgatga agtcattgga tatgttggct
     5041 atttcgagtt actgaatgga ttcaaaaaaa ctgtctattg gaccaaacaa gaaatcgaag
     5101 ctcatcggat tgctaacaat aaagatcgcg ataagacaaa gttaagtggt gtgtgggcat
     5161 ctgattacaa tgcaatggca cgaaaaactg ttttgagaaa tcttctttct aaatggggaa
     5221 tcttgtccat cgaaatgcaa gaagccacca catcggatga gagagtccaa agggttcaag
     5281 aagacggcag cattattgct gaaacagaag ttgaagaaga tattcctgaa agaaaagaag
     5341 cagaggttat atctgaagaa aatgaagatg tacaaactgg attatttgat gcatctaatc
     5401 cgccgttaaa caaataatga gggagctttc tcccttgctt ttctagagga gaaatacgaa
     5461 tgaacacagg atatgtcaaa ttacatcgaa aagtgatgaa ttcattcgtt tggaccaatc
     5521 cttatatgta taaattatgg agcttgtgtt tgatgaaagc gagtcatgag aatcgcaaga
     5581 tactttttaa tggaaaagag atacaagtga acagcggaga attcgtcaca gggcgcgatg
     5641 ctatcacatc tgagatgaac gaaggcgtga aacgtgaaca tcaagtgaac agcggttctg
     5701 tatggagatg gctaaaacaa tttgaaaaaa acggaatgtt gaacatcaaa tcaactacga
     5761 aatacagcgt tatatcaata aaaaactggt ctttgtacca aagcagtgaa caacaaatga
     5821 acatcaaacg cacaacaagt gaacaacaag tgcacacaaa caagaatgaa aagaattata
     5881 agaatgaaaa gaataataaa acatcatcgt cgcaaccacg caaaaagcgt gtttacgaca
     5941 ccgactcagt ttactacatt ctcgcggagg agttattcaa acagatttgt cagaatcagg
     6001 aaatcaaaaa gccgaatctg caaagttggg cagataacat tcggaaaatg atcgagatcg
     6061 acaagcggac cgaaaaccag gtacggggaa tgattgaatg gagtcagcac aatgtgtttt
     6121 gggcatcgaa tattttatct gctaaaaaat tgcgagaaaa atacgacaca atggcagcgc
     6181 aagcaaatcg tgattataaa acaaaacaaa ctaaaacgct tgaatacgag aaatttagca
     6241 cagatgagtt gcctatttga gaggaggcgt aggcatggaa actgttgggg aaatcatgga
     6301 aaagctgata cagaaagtgc ttgtccaacg tggcgaatgt cctgaatgcg gacagccttt
     6361 gtatggatgg cgtacgaaga accctgatgg ctcagaacgt tgtaagccaa cctgtatgca
     6421 atgtgggtat aaagcattgc gagttcagga agacttacaa acagaacgaa tttacaacga
     6481 gagcctgaaa gcaagagcaa tcaatttttt caaaggtggt tctgttgtgc ctaatcaagc
     6541 gttgtttgat tgcacattgc agaattatca aattgtcgat caagaaacaa gacaagcggt
     6601 tgaagtaacc aaacgctttg ttaattcagt cttgttagga aatccaagtc accttgtttt
     6661 aactggaaaa caaggaacag gtaaaagtca cctagcgatg gcagcggctt gggaagtctt
     6721 gaagcgatca aactacgata aaaaaatctt atttatcggg ttacaggaaa tgctggatca
     6781 aatcaaattt tcgtacaaca atcctgaact cagaaaaacg attgagggat cgttgattgc
     6841 agatatcaaa acagcggatt tagtcatcat tgatgatatc ggttcagaac taggaaaaga
     6901 tgcatcagat agtcgagcgt ttggcataaa cacgctaaat tcgttcttgg atgcacgaca
     6961 gaacctagca acgattatca caacgaactt gcttggtgaa gaactgaaaa aagcttacgg
     7021 tacgagaaca atatcaagaa tgtttgtcaa ctctgatgga tttacgatgg tattttctca
     7081 aacagcagac aagcgcataa aaccagtcaa aggagcgatc gcatgaattt gactaaacaa
     7141 gaaaaaagaa tctgtcgtca gataatgttg caaattatag gtgatcgtga catggacgaa
     7201 ttatctcaac aagagagtcg aatggttcgg aagataagta agctaatcgg tgcagatacg
     7261 atggatcgat ccaagccatt acccaaactg aattttgaat catttacgtt tgagaaatat
     7321 agccatctga gagaggtagg ctatagtgta aatgctattc gaaaagcact tggggtagga
     7381 agtagtgtat gggcaaaatg gcggaaagaa aatgtaaatg aggtaaatca aaatgattaa
     7441 gctaataggc aaagagacgc tcaaggttta ctatgtagcg gaaacaaagg cagaagtcag
     7501 ccgttggcta ttgaaaatgt ttaataagac tagagccaat gcttcagtaa gtcagaaagc
     7561 aagtgtatca gaaacaggca tgcctgaagc aatgtggatt tcaaggagga actgagatgg
     7621 caatgatacc gaagtttaga gcgtgggata gagaaaaaga cagaatgatt tatcctagca
     7681 cgacaggagt ttgttttgaa atgaatgatg atggtataag tgtgcttgat attgatgtag
     7741 attatcccaa tgactatggt tttcccaaaa tagcttcaat cctcatgcaa tctaccactc
     7801 tgaaggataa gaatggtgtg gagatatttg agggggatat agcgcttatc actcataaat
     7861 caacgaacta cgccgatact tattggcaca gttacgtaga agtttttaga gcaaaaaacg
     7921 gagcctacag aattcgcggt gaacacatct acgaaacaga attatatagt aatagaaaac
     7981 aattgacggt agcggggaat gtttataatt caccagaact tttgaagaga gatcgattta
     8041 tgactactac cgaaaatcca gagttattga aggaggaaca gcgatgaata aacaggaatt
     8101 gattgataaa tatactgcag aaatatctag attaagacct tattgcccta atagacattt
     8161 aattagcgat caacttaaat atggtcttta taaagaaatc ctagaagatt taaaacaact
     8221 agacgaaccg cagaaaccag cggtgcctaa gtttgtggcg gaatggatag aatacgctaa
     8281 aaagaaaggc gatagtctag ctatttcatt caagccgtgg aacctctacg gtgttgagta
     8341 tagcaagact gatagatgga ttgaagataa tcaaggaacg tttgctcgtg cttggctaat
     8401 tggctacgag gtcgagaaag agccgttgta ttatgtgaag ttgccagttg tgtattttaa
     8461 tcattgggat ttagagacgt atctaatgaa agatgataga ggaaatataa caattgcaga
     8521 caacaacgat tttgatgata tgaaatttac ggaatcagaa ataaaagcaa ttgatgaaag
     8581 atactggcca tttgctgtgc cagtggaaga ggtggcggaa ggatgagcaa tcttaaagtt
     8641 ggagatattg ttttttacaa aggtgacgaa ggcatcatcc gagaagttat gacggatgga
     8701 aaagaaaatt tttatagggt agatgtcagc agaaaatcta tactctacct gtatgaagac
     8761 gaattagatt taggtactca aaaactcaac gacaaccaga aaatggtgtt tgattatctt
     8821 aaatcaaacg ttgaacaagg tggcaaatca gttatgtatt cgatattcct tctaggtgat
     8881 tgggactctc gaattggagc agcaagaaac gttgatatag cttactgtgg attaaatggt
     8941 aagcaggaag cagaagtcct atcagcattc gctcaatggg gattagaaca ggaggaagcg
     9001 gaatgaaata cgaaatacca ctaagtgaag cagacgtcca gtcaattatt aacggtcggg
     9061 aggttaataa aaaacttcct gatggtactg aattagtcat cagacaaagt tatttgaaag
     9121 atatggcagc tccagtatta attgatcgtt ttaacgtgac tgattctgtg gtagagaacc
     9181 atttaaaaga atttcgatca agtatagacg acactttcag attagggagt tgattgacaa
     9241 tgaacaacag acatcgcaga ataacaaaac taagaaaaca ggaactgaat gtactaaaga
     9301 caaagtttga aaaagaatat ggaatttcag cagaagaaac atataaagtg gcaagtcagt
     9361 gtgttgctga tgcgagtaat gctattcgta agtttgggat ttcgatatta aatgatgatc
     9421 gtaaatggga ggaaatgaga tgaaactaaa agacggattt tacgctagta gtcacggcat
     9481 cggcggttta atgctagata tgccgacaaa gaatcctaaa acacgtaaga aaccaaaagt
     9541 caaagtcggt gacatggttc gctgcgaagc agaagggttc atctatccat ttcgtggata
     9601 tgtagaacac gtctataatc actcagcaat cattcgtatt gaaaacacga tggaatgtga
     9661 taaatggaca gctaaaagca aagagaattt agcagtggtg agattggtgg atattgaact
     9721 aatcaatgac aaataaaaaa gccggatcgc tccgactgat tcaataaatt ccacaagttt
     9781 attatatcac ataaaagggg cggtttgact tgatgcaatt gttacgagag gtagctttca
     9841 aacagacaag atgtaatgcg agagatgtgc tgaagaactt tcggcgtttg gagcggatgg
     9901 caggtcgctc tttgatagat attaagtcgc cgattattac ggacatgccg aaggcaccga
     9961 agcacggcaa taaggcagaa gacgcgatca ttcagatgat ggatatagaa gcagagagag
    10021 acgcgatttt agcggctttg atggcgctta gtctgattag tcgtcagata ctctactaca
    10081 gcttttgtgt gccagatagc ttctcaaact acaggattag ccgtgaagtg ggttattcag
    10141 aaagaagcat acaacggatg aagtcggaag ctctgataga gtttgcagaa gcatataaac
    10201 atgggaaaat aattgcttat aaataggagg actatataat gtggaaagat tatgtatcat
    10261 tgaaagagtt gaaaaaagat cttattttca aaaaaatcgt agaatggtca gagagtgaat
    10321 taattttaga agatggaacc aaaatggaag ttgtatgtag cgaatcagat tgctgcgcat
    10381 gggccgaagg tgaattcaaa aatgtaaagc ttgatgcagt gattacagat ataaagattt
    10441 ttgataaagg taaccgtctc tataatggcg atggacatag ttcttatgcc gaagtcgttg
    10501 tttatcataa tcgaaatgag attgctaaag cggaatgtac agcaaacgat gggaatggag
    10561 gctactatta cagtgtctgt gctttaaaag tcaaagaaaa actttgtgta gtgactgatg
    10621 cataaaaaat ggcggttttt tggcggaatg atggcggttt ttagctattt accagtgata
    10681 ttatggtagt gtcgaaagat tagtgatagg tctgagacaa aataaaatgt aagggaggaa
    10741 atctccctca tcgtttaatt aagcttcgat agacagcagc ggaaatatta agaataagga
    10801 tgtgaatttt aactccttct aaattgttct tattatctat catccgttgc tgtctattgt
    10861 tattatgtca ctgtggcgga aagggtagac gcttaaaaat aaggtcaata cgtcgaggga
    10921 tagccttaac gttttatgat ttgaccatgc aaggttcgat tccttgccag cgacttagac
    10981 gaaaggtagc ttaaatatta accacatcaa catcgtcagc gagttgataa aggtaactag
    11041 cagactctag gatttaagcc agtcttaccg attagtctgt tatagtagca agcttgctag
    11101 aggtagctcc tctggttgac gtgcagctca gttggtagag cgcctgactt ttaatcagga
    11161 ggtcgccggt tcgaatccgg tcacgttaat aaagaaccta cggaaacaat caatcttatc
    11221 ggatgccgat tggttggctg aattaagcag tgtgtctgtg aatcagttaa taggcataaa
    11281 atactagcac agacgtgtgc cactctcagg tgtaggttag gagagaaaca ttagcaaccg
    11341 agggttggaa atgggcgctc aaagtacacg agcaaggcga ggtcgatagt aatcgatgga
    11401 atcggtgtag gttgctatta cataattgat tgggttagat tgagttttgg gattcggtac
    11461 aaatgaatcg tcaaatgact caagcacagg atcggaaacg tccctgcctg tgcattacat
    11521 attagatcac tcgttgagtg gtctttttat tttagaaaag agaggatttt gaaaatgaat
    11581 catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga gaatgagatt
    11641 gctgacctaa gtggagcagt acctgagttt agtgttttca tagtctggtc atgcaaaaca
    11701 ctacagaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta ttttgaaatt
    11761 acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa atcaaatgaa
    11821 tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggaa tgatctcatg
    11881 aaacaatata ccgctaaaga tttcgaggaa atgaagcgat taaagaagga ctatgaagaa
    11941 gttgatatgg agctaactgt tggagtcatt caacgaagac tgcgggtcgg attagagaca
    12001 gcaaaggcta tttacaatga tctaaatgct attgaagaga agaatggcta atgaggaact
    12061 actggtatat atcgctaact aatgaatatc ctcgaaccat tgatgattgt tcagtgcgtg
    12121 ttgtgcgttc tgtacaaatc aaagggaagt actctattgt cgaaatgggg agagaagcag
    12181 aaccatgtga gattgatgcg tgcaagctcg tttattgcgg tcatggtttc tatgatgaac
    12241 caaacattca aaataatatt aacaagaatt tgagggatta gaatgcaaga aatggcttaa
    12301 aaatctccaa tcaggacaaa tcaagttaga caaagtttcc gatttaaaga tattaattga
    12361 agcagatcta atgttgaaag atattgaaaa ttagaaaaca aaactcaacc taagaagatt
    12421 gcgaggtggt gtgtattgaa tggcaagaca acgtgatcca agacgtgatg aagccaaaag
    12481 aatttggtta gaatccaacg gagaaaagca gttaaaggaa attgcatctg aattaaatgt
    12541 ttcagattct caggttagaa aatggaaatc gcaagacaaa tggagcgctg aattgaaaag
    12601 taacgttacc aatggcaaaa gtaacgttac taatcaaggt ggcgctccta ttggtaatca
    12661 aaatgctaaa ggtaacaaag gaaatagccg agcttctccg ccagtgggta ataaaaacgc
    12721 tttgaaaaca ggcgagtatg aaaccatatt ttttgagaca ctaagtgatg aagagaagga
    12781 catctattct agtctgaatg ataatccttc ttttgttttg tctgaagaaa tacgtctact
    12841 taagataaga caatttcgta tgatgaagag aatccaacaa gctgaagctg gactaaatga
    12901 tgaagaagtc gaacgattgc agcagctaag aaagattaaa aatccgattg aaaaaaatgg
    12961 taaaaagcta gaaatcaagc gtgaggttat gcaagatgtg cagattagca gaaaaaaaca
    13021 tcgcaaaatt gatgatattc tttcaattga agattcattg actcggatta gcaaccagtt
    13081 agctaaagcc atcaagcaaa tgaatgaact ttatatgaat gaatacagaa ctgatttaat
    13141 taaagctcag actgataaga tccaagctga gacaaatgaa attggcggaa ataattcagg
    13201 tgaagaaata gaagaatgga aacaggcagt tttaaatgcc gcaaacaaac gggcggtgaa
    13261 agaaaatgaa taatgaattt attccttttg ctgatattgg ttctgccatt gattattact
    13321 atgataaacc agtagctttt tgccaagata ttttgcattt gaatcctgat gaatggcaag
    13381 aaaatgtttt aaatgattta gctgaatttt caaaggtttc tgttcgttct ggtcaaggag
    13441 ttggaaaaac agcattagaa gcaggagcaa tactttggtt cttaacgtgt cgaccctacg
    13501 ctaaagtaat agcaacagct ccgacaatga agcaacttta cgatgtactt tgggcagagg
    13561 tagctaaatg gttaaatgat agcttgatca aaaacttact gaagtggaca aagaccaaaa
    13621 tttatatggt tggtgattca gagcgttggt ttgctacggc tagaacagcg actaaaccag
    13681 aaaatatgca aggttttcac gaggaccata tgttgattgt ggtagatgaa gcttctggtg
    13741 tgtctgatcc aattatggaa gctattcttg gtacgctatc aggttttgat aataagctgt
    13801 tgatgtgtgg aaaccccaat aatattgaag gtgtttttta cgattcccac aattcagacc
    13861 gtgataaata cagagttcat aaagtatcaa gctatgatag taaacgtaca aacaaagaca
    13921 atatagaaat gattcttaaa aaatatggaa aagaaagtga tgttgctcgt gtccgtattt
    13981 ttggagaatt tcccaaaggt gcgttggatt catttatcag tcttgaaacg gttgaattgg
    14041 ctacagaaaa acaaattagt gattctttag tcaataaaac aacggttgct catattggtg
    14101 ttgacgtagc tcgatatggt gatgattcta cgattctctt tcctagaatt gctaccaggg
    14161 cattggagta tgagaagtat tcaaaacgta gcaccatgga aacaacagga tatgtcatca
    14221 acatggccaa gaatctaatg agtcaatatc cgagtattga taaagtgatg attaaagtcg
    14281 atgacactgg tgtcggaggt ggtgtaaccg accgcctaga agaacttata gaagacaaac
    14341 attatccttt tgaggtgttt ggagtgaata acggttcaac atcagaagac gatttttacg
    14401 ataatttagg tactcaacta tggggaaaca tcaaggaaat gttagaagaa aatatgacag
    14461 caaatcttaa cggagaacag cctgttattg aattgccttc tgatagttcg ttaatcaaag
    14521 aattaagtac tcgcaaattc aaaatgacaa gtagaagtcg tatacgttta gaaagtaaag
    14581 atgatatgaa aaagcgaaat attggtagtc ccgatattgc tgacgcactg gctttagcgt
    14641 tttatgagcc accaagtcac tatcaattta ttcaatttta ggaggtgagc ttttgattag
    14701 tacagtatta tctttagaga gatataagaa gctacgtgtt aaatatgcaa cacaaattga
    14761 agatggaatg tttgatccga atggttttat agaagatatg aaaccatttt ttgctgatcg
    14821 cgaaagaaaa tatctagctt atactagcga aaagaatgaa atagataata gaccaaagcc
    14881 taacactgat attgtaaaag ttaataataa acttcatgct ggtatgtatt caattgtcgt
    14941 agaccaagca gtcaatcatt ttactggtat acctatcaaa tgggattacg atgtatcgga
    15001 acaaaaaaga acactcattc aaagattaaa agataaattc ttaaaaaatg atatagaact
    15061 tccgacagtt ccagaagctt ttaacaaatt aacaagcaat cttgattcca tgagattcgc
    15121 aatgcttgat tctgaaactg caacttttca aggagcttgt ggagtagctt tccgattgtt
    15181 agagcctgtg gaagaagatg atggttggaa gttaagagca agtaatattg aaccttggag
    15241 agcagaaaga tatggaaatg ccggaatcta tatcaaagaa aagtatgact cttaccagaa
    15301 aaaatttttt caagaaatga aagttatgac aagaaataaa attctgacat atgcttgcta
    15361 tggtgatttg aattttgtta caagcggaac gttcaaaaaa attgatgaga ccgataaccc
    15421 tttggggacg attaccttgg cagaatttaa aaataatacg aatcgttatt gtgattttga
    15481 agtagctgag gaaattggag atgcaattga tcgtgctttg tctgatcaac aaaacgaaat
    15541 cgaacaattt aagcttgcgt acatgcttgt tactggcaca acaatgagca aaggcactgc
    15601 taaagaaatg atgaatcagc tagggattat caacttaaaa gaccctactg caaaagctga
    15661 atatgtcaca aaaaatttag caaaagattt caatgaatat catatggatc tattgaaaaa
    15721 acagttttac accatttgta aagcaatcga ttttaacgat gaggtgttta aatcaaacag
    15781 ttctggagaa gctcgcaagt ggcaaatcat tagcctagaa gcgaaaacaa atactaaaga
    15841 gcaatatttt agagaaggat tgaaagaatg cgcagaaaca attgctgctt ttcttaagtt
    15901 tcatgacaaa gtagaaattg agcctgaaaa aattattttt actttctcta gatctttgcc
    15961 aactgattta agttatttag ctgaagcttt acctaaactt gcaccatacg tatccaaacg
    16021 aactattcag agccaaattc catttgtaac agatgttgat tatgaaaatg aaatgatgga
    16081 attggaaagt ggaagtgctt atccagatag tgaatacaat tttggcggag gtggcaatag
    16141 tgacgataga aacgaagtat tggaccaaac gtcgagaact ggaggatcaa gcaaggctca
    16201 aacaagaaaa tcagacactt aaaaaattaa ctagtgtatt tcctgaagca ctgaaagaga
    16261 tacaagcaaa actattatca caagctgact tacacaatat cacttatcca gaaatgatgg
    16321 agttttatag tacaagtaat cagaaaaaat atcgtgaata tgtggaaaaa aattataagt
    16381 cattaaaaat gtatgatgca aaatacaaag agtttatcga tgaatttttt ccaccatttg
    16441 actatgcaaa agtcaatcgc ttattacaaa tacgatcaga tgtatttaaa attcttgcag
    16501 aatatgcgat ggatgcagat gtgaatcaat atttttctga tcgcttagag gaaattcttc
    16561 aaagaacata ttcttctaat gctaatgttt ttgttcaact tttaaacgtt gatataccaa
    16621 attatttacc agaaaatgaa attaaacatt atttgaacta tccatggtca ggaaagacat
    16681 tttcaagaag actttgggga aatatttcat cgcttgaaca gaaactctct aatgctattg
    16741 taaaaagtgt tgctagtgga gaaggcgtta tacacgcatt aaacaccatg agattagact
    16801 cagaaatttg tgacatgttt aagttagaag aatcaaagta taacaaagcg atagaaaatc
    16861 tcgttcgaac ggagtatgca aaatttgcac aagatggtat tgaaaaatca tatttagaaa
    16921 caggtattga ggaatacaac gtattgactg caaaagatga gagagtttgc cggatttgtg
    16981 gaggaaaggc aagtaagaat ccctataaac tgaaagatgc tgtcataggt gaaaatcgag
    17041 cacctttcca tagtcgttgt agatgtacgg atgttcctaa tttaccaaaa ttaggaaagg
    17101 atattgatga agaatatgac cgtttatttg gcgatttatt agatgagttt gcacatgatt
    17161 cttttggaat taatttgaaa cggaggaagt agaatgaaag atttttttga agcagtacta
    17221 acaattaatg taaatgctga tattgcagaa gcctacaaaa cagctattga gtctgagaac
    17281 catcctaatg gcttgagaga ccattggaat ggcaattatg cctacgtggt tattggcgat
    17341 caaactgtta attatcaaga taatactcca gttgataaga ataccgttaa tttaacgatt
    17401 caattattat ctcattcatt accaaattta aaagaaacag ttgattggta tgaaaagatg
    17461 ggatgtattg ttgttagaac tgactacaaa gaaggaaagt ctagtaatta ggctttttta
    17521 ttttgtccga aatgacgtta aactagcgca atactgggct tggttgaatg gtggggcgca
    17581 actattaaaa ctcaaagcaa tgcggggcgt gaaaacgaat cgtggggcga aaggagaatg
    17641 agcatgaaac acaaatcatt aatgccaatg aatttgcaat actttgccga aggtgacgat
    17701 caaaagtttt cttttgatga cttcaaatct tttgtggaat ccaatgaaga agctcaaaaa
    17761 tttattcagt cacaatctca aagtgttgcc gacaaacaat tggaagcttg gaaacaaaat
    17821 aacttagata aaattaaaca ggataccatc aaggaatatg aggaatctaa gaaaaacaag
    17881 tcacctgagc aaattcaact ggaaaaacta caagctgaat ttgaagcaga aaaagcgttg
    17941 cgtgtgacaa gtgataataa agcatttgtt gcagaacaaa ttgctggatt agaactagat
    18001 ggagagttaa aagagtctat ttctcaattt atgctaaata atcttgttag ttcggataca
    18061 gatttcacta agaatgctgt tgaaggtttt acaagtgtct tgaatgcaat taatgagaaa
    18121 catgcagatg cattaaaaga actacaaatg aagtctgcat ttggtggaac tcaacaatcg
    18181 aataaccagg ttcagcagaa caatgaaaca ttaacaaatc cagaagaaca attaggacaa
    18241 attcttcaac aatttaacta ggagagtgaa aaattatgaa aaaaacatct ttaaataatt
    18301 tagagtattt ggatatttca ccagcgatta atgctatgca agtaccaaat acaccttttt
    18361 taagctattt atttggtgct ggaaaaacag agccagcaaa ctcgacagaa attaaatggc
    18421 gtgaatatga tatcaacaac gatgattctt ctgaaaaact tgagggcgga gaatatccag
    18481 atgctgaatc aggtcgaact tggtttaaca actatactga aatttttaga aaatcaacct
    18541 ctgtatctgg tacattagat gctattaatg tgaatggagt cggaaatgaa ttaactaatc
    18601 aagtagcact acgtggtatg gaaatgaaaa ttgatttgaa ccgaaaattg attactggtg
    18661 taaaagctga tgaaaatggt tctaaaggtc gtcgaatgaa tgggattttg aacttgatca
    18721 attcagcaaa taaggcagaa acagccactg cgggtgcagt aacaagaaaa gatatcgata
    18781 ctttatttaa attgatgtat gaaaaaggtt atatgggaga aaaactatgc ttgatttctc
    18841 cagatatgca ggagttaatg actgatgagt tagatggaaa atcaacaaaa attgttcagt
    18901 tcggggaaag agtaactttt ggattgcaaa ttggaaatat cgtgtctaat tacggtacag
    18961 gtattgctct actagaacca tcattgccaa aaggaacaat tgccgcaatc gatactaatt
    19021 atgtgaaact acgtccatta cgtgaatgga gagcagaaga acttgcaaaa acaacggatt
    19081 ctagacgtat cggtcttgta ggtgaatact ctcttgaata caatgcttca aactctgggg
    19141 caattttaaa tttaaagtct gaataaaagg gagttagtac tccctttttt gataggagga
    19201 attacagtgg taaaaaaaga tgaaactaaa aaagatgaag tcgtgaaata tagagtaggt
    19261 aaaactaaaa attttgttgg gtttgttcat cctaaaactc gtagatttat cacagcagat
    19321 tcaaataacg aatttatcat ttctatagat gataaagaag caattgcaat tttggaagat
    19381 gcaattgatg ttaatgaaat ttaggaagtg atctgatgga tgaatcgcta aaaacggaaa
    19441 tcattgagtc tacaaaagaa gattttccag atttgagtga agaacgcata actaatttat
    19501 tagaaataat tttgctagaa attgaatcat ac
//
LOCUS       DAFFSV010000044        19385 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFFSV010000044 DAFFSV010000000
VERSION     DAFFSV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.044"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(49..1269)
                     /locus_tag="LR110_001427"
     CDS             complement(49..1269)
                     /locus_tag="LR110_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBL4822394.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(1284..2372)
                     /gene="murG"
                     /locus_tag="LR110_001428"
     CDS             complement(1284..2372)
                     /gene="murG"
                     /locus_tag="LR110_001428"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBL4822395.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(2398..3759)
                     /locus_tag="LR110_001429"
     CDS             complement(2398..3759)
                     /locus_tag="LR110_001429"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBL4822396.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(3773..4735)
                     /locus_tag="LR110_001430"
     CDS             complement(3773..4735)
                     /locus_tag="LR110_001430"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBL4822397.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(4761..6953)
                     /locus_tag="LR110_001431"
     CDS             complement(4761..6953)
                     /locus_tag="LR110_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL4822398.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(6954..7355)
                     /gene="ftsL"
                     /locus_tag="LR110_001432"
     CDS             complement(6954..7355)
                     /gene="ftsL"
                     /locus_tag="LR110_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBL4822399.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(7360..8319)
                     /gene="rsmH"
                     /locus_tag="LR110_001433"
     CDS             complement(7360..8319)
                     /gene="rsmH"
                     /locus_tag="LR110_001433"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBL4822400.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(8340..8771)
                     /gene="mraZ"
                     /locus_tag="LR110_001434"
     CDS             complement(8340..8771)
                     /gene="mraZ"
                     /locus_tag="LR110_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBL4822401.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(8941..9309)
                     /locus_tag="LR110_001435"
     CDS             complement(8941..9309)
                     /locus_tag="LR110_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBL4822402.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            9480..10436
                     /locus_tag="LR110_001436"
     CDS             9480..10436
                     /locus_tag="LR110_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL4822403.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10509..10625
                     /locus_tag="LR110_001437"
     CDS             10509..10625
                     /locus_tag="LR110_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBL4822404.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(10680..12362)
                     /gene="recN"
                     /locus_tag="LR110_001438"
     CDS             complement(10680..12362)
                     /gene="recN"
                     /locus_tag="LR110_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBL4822405.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(12380..12829)
                     /locus_tag="LR110_001439"
     CDS             complement(12380..12829)
                     /locus_tag="LR110_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL4822406.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(12936..13754)
                     /locus_tag="LR110_001440"
     CDS             complement(12936..13754)
                     /locus_tag="LR110_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBL4822407.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(13763..14653)
                     /locus_tag="LR110_001441"
     CDS             complement(13763..14653)
                     /locus_tag="LR110_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL4822408.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(14655..14879)
                     /locus_tag="LR110_001442"
     CDS             complement(14655..14879)
                     /locus_tag="LR110_001442"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBL4822409.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(14884..16221)
                     /locus_tag="LR110_001443"
     CDS             complement(14884..16221)
                     /locus_tag="LR110_001443"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBL4822410.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(16222..17076)
                     /locus_tag="LR110_001444"
     CDS             complement(16222..17076)
                     /locus_tag="LR110_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBL4822411.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(17173..17622)
                     /gene="nusB"
                     /locus_tag="LR110_001445"
     CDS             complement(17173..17622)
                     /gene="nusB"
                     /locus_tag="LR110_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBL4822412.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(17615..18040)
                     /locus_tag="LR110_001446"
     CDS             complement(17615..18040)
                     /locus_tag="LR110_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL4822413.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(18286..19350)
                     /locus_tag="LR110_001447"
     CDS             complement(18286..19350)
                     /locus_tag="LR110_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL4822414.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
BASE COUNT         5311 a         4229 c         3231 g         6614 t
ORIGIN      
        1 aattactggt ttttagaaag tacattctta ctttttaaag ttgttttatc aattttcatt
       61 taaacttgac ggagttgtac ttggattatc ttctatttga cttgtttcac ttgaatcatt
      121 tgaatcagat gatccgtttg tgctagacga tccaggtgtt tgtgcttttt gcgtttctgc
      181 ctcttcactt gattcgtttg tttgatccgc attttcttca ctttcagttg tttcttccga
      241 ttggcccgat tcttccgttt cttgagtgct accgtaagga tgcgagaaga taccaacttc
      301 catgtcgatc acaccttttt catccatatc tttggctact tgcgaataat atttcatttg
      361 gttcgcaaga ttattgatat ttacaataac ttgattgccg tcattcatat tcaattggat
      421 cagattttta ttgcttgctc ttggcgtgta cttgatttca gaaattgctt tttgtaattc
      481 tgatgataac tgattgtatt gctttgttaa ttctttgatt tttgcagagt ctttgaactc
      541 ttccaagata gggagatttt ttgtaggatt tgctactttt tcatctaata ctgttccatt
      601 ttcaatcact gggtagtatt gattgttttt tgcgatcaaa gcaatctctt tatattctgt
      661 tacatccaaa tcgaattcat tcatcccttt gaaatgtata tttgctgttt cgattcttgg
      721 ctgtgctttt ttaaggcgat cgatataagt agatcgatga aaatactgtc cccaaacatt
      781 ttctcctaat gacaattttg tatcagagat gatttcttgg ctgttgactg tcttattgcc
      841 tgatactgca actgcttgta atttgctata aggagaaaca taatagatca aaaaaatcag
      901 cggtataccc aatacagtga tgatcaaagc taaccgacga aatagtactt tgtgccgttg
      961 actttttaaa ttgggcagcc ggttcaaaaa agaaccgtta tatggtccac cgtttttctt
     1021 ttgcttgatc tcttcaaaaa attcgatttt tttctcggac tggctttctt cttgctgttc
     1081 ctcttgaacc gttttttctt cttcagaatc tgaaaggggt gtatctaatg ttatgtcttc
     1141 tttttttggc tcttggtttt tcatctcttg ttctgccaga tattttcggt tagccttttg
     1201 ccaaggagtc aaattgtttt catcagaaga cggatgagac aatttttttg gttctttttt
     1261 cttactcact aagaccaccg cctttatttt ttgattattt gttttactaa gtcataaaga
     1321 cgttttgaag catcagggat tccttgttct ttagaagctc ggcacatttg cttttgcaat
     1381 tcttcatcac ccatgatttc gttgactgtc tgacttagtg attcacctgt tagttcatta
     1441 tctgcaatca tttttgctgc tcctgcgtga acaagactca ttgcattttt agtctgatga
     1501 tcattcgtaa catatgggct gggaaccaac actgccggta accccagagc tgtgaattct
     1561 gcgatcgaag ttgcaccagc acgccccacc agcagatcac tggaagccat gacctctgcc
     1621 atatttttga tataaggctg gacactaata ttttgtattt caggatttat tttatttagg
     1681 atctcttcat aatatctatc tcctgaagca tacaaaactt ggtaagaaga aggaatatat
     1741 tcacttaaaa aaactgatac tgcttgattg attttaagtg ctccttgact accgccaaaa
     1801 atcaagactg ttttcttttc tggatccaaa tcaaatgttt tcaaaatatc tgatttttta
     1861 gtatctgcta cttcttgtgc tcgaggatta cctacaagaa ctgctttgtt ttcaggaaaa
     1921 aaggaagccg catctttaaa tgaaagagcg atccgatcca catatcggct taaaaattta
     1981 ttggtaacac ctggtacact gttttgttca tggataactg ttggaatacc cattttggct
     2041 gcagcgtaca ctaccgctcc ggaaacatat cctccagtac cgataacgat atctggttga
     2101 aaatcactga ggattttttt tgcttgacgg atactcttaa gaaacaatcg gaccgtcttg
     2161 atgttatgca tggaaatttt tcgtttaaat ccttgaatct ccaaggtatg gaaaggcatt
     2221 cctgtatcag ggacgatttt gttttctaga cctcgttttg ctcccacata catgaattca
     2281 gcattcggtt cttgagtctt tacataattg acaaaggcaa gagctggata aatatgtccc
     2341 cctgttcctc cgcctgtaac caaaattttc attttttatc gctcgcttct actttttcta
     2401 ttttaatcgg ttcacagctt ccatgaatct gtttcctcta gtttcaaaat ttgggtattg
     2461 atcccagctg gcattcgctg gtgataataa aactacatca ccatctgtac tgttttctaa
     2521 tgctaaagga actgcttgtt ctacagaatc ggctgttact attgtctcga tgcctgcttt
     2581 ttttccagca tccatcaact tttcttttgt ttggccaaat acgaccattg ctttgattcc
     2641 ttttaatgaa ggaattaatt catcaaatgt atttcctctg tctaatccgc cagccaataa
     2701 cacgacctta gacgtttcaa atccacttag agccatttta gtcgctaaaa tattggttgc
     2761 ttttgaatca ttataaaatt ttctgccttg gatttctcct acatattgcg tacgatgtgg
     2821 tacgccatgg aaaaaatgca acgtttctcg tatagcttca tttgatatac catacaattt
     2881 tgctacagaa atagcagcta gtgcattttc tacattatgc ttacctggta cgccaagttc
     2941 agatatttcc attatttttt cttttttata gtatattgag taatcgtcag catacacacc
     3001 gtcttccaac acttcttttg tcgagaaagg cagtacgcgt gcttttgttc tttgcgctaa
     3061 ttcttgtagt tctgactgat tccagttaag gatcagataa tctttttctg tcatgttttt
     3121 ttgaaggttc catttagctt tgacatattc ttttcttgta ccatgataat caatatgtgc
     3181 ttcatagata ttggtaatca ccgctatatg agggcggaaa tctgtgattc ccatgagttg
     3241 gaagctagac aattccatca caattttatc ttctgatttt gcttcttgcg ctacaccact
     3301 tgctggatac ccaatattac ccgctaaacg tgcaattcct tgatctgctc ctgcattcag
     3361 tagtagtcct gtcatcgttg ttgtcgttgt ttttccgttc gttcctgtaa tcccaataat
     3421 tgggcattcc gctacttcgt aagctagctc aacttccgta attaccggaa tacccatttc
     3481 ttgggctttt gctactaaag gatgactgta gggaatccct ggatttttga ccatcaatga
     3541 aaattcttca tccaacaatt cgattggatg actacctgtt attactttta tccctaaaga
     3601 aagcagctct tgtgcttccg gattttcgtc aaaaggtttt ccatcattaa cggtgacaag
     3661 tgcgcccaat tcatgtaata atttggccgc acttactccg ctttttgcga gtcctaagac
     3721 aagcactttc ttattttcat atgttgatat ttttttcatt tcccgtcatc tcctaccaaa
     3781 cgatccataa tgtgatcaac gaagtcacaa tgctgatcaa ccaaaaaact gtatcaatct
     3841 tccactccga ccatccgcac atttcaaagt gatgatgaat tggagacatt ttaaaaattc
     3901 gttttcctgt aagcttaaag gaagtaactt gaagcatcac acttgctgtt tccataacat
     3961 agatcaaacc aatcaaaagc aatgtccatt cttggtttag cattatagaa attgccgcta
     4021 aaagtccgcc caacgctaat gaaccgacat ctcccataaa aatcttggca ggttttctgt
     4081 tgtagacgaa gaaacccagt aaacctccta aaacacttaa gcaaatgatt aatacatcat
     4141 actgatgctg atgccaagca attagaccgt aagctgcaaa agagatactt cctaagccag
     4201 caactaatcc atcgatgcca tctgtcagat ttactgcatt tgaaaatcca acaagccaga
     4261 agagagcgaa aataccataa atgattccta aaggtagatc tagtccgaag aaattcaata
     4321 cacctggata accttcagaa cggtaaacaa gataaaagat gattccccct agaatctgtc
     4381 caatcaattt ctgcttcgag ttcagtccca tgtttctttt cttgaatatt tttataaaat
     4441 catcaagaaa gccgattact ccgtataacg ctaatacaaa aagcaagatg aaaagagtag
     4501 gagttagctg tttttgccaa gctccaaccc aaattcctgt caaaatagag ccgatcagaa
     4561 acactaagcc gcccattgta ggagttccgg ctttgctatt gtgccacttt ggtccctcct
     4621 cacgaatcgc ctgaccttgc tttttcattt gaaaataacc aataaatagc ggcattgcgg
     4681 caattgtcat tgcacaactg ctgacaatgg gaatcaatgc ttgtgtccat tccatgctct
     4741 tttctctcct atttctcttc ttattcttct aaagtaaaac taagtttgtc ttcagtgatt
     4801 ttttcatatg gagcaagttc ttgtttgaca caataaccat ctccatcaaa actaacttca
     4861 ataccgagga ttttccccag cttcatgata tctgcttttg accatcctgt tacgtcaggc
     4921 atcagcttat caccaccagt atataggatc agtttttctc cagagatcaa ctgatcaccg
     4981 ttggcagtag attgtttttg tactttttta ccattaccga tcactacagg ttgcagtcca
     5041 cttttttgag catcagctgc tgcaacgtcc gcttcaagat tacgataatc ggctacggat
     5101 actttttctg tttttgtttc tgtatctgaa tcttcttcag tttctttaaa atccattgcc
     5161 cgtttcatca atggatttgc gattttagcc aatgccattc gatcatatgt cttagggtgt
     5221 ttcattgtta gatacaagac atagtctgga tcttcagaag gaaccatctc cacaatcgaa
     5281 tacaggtagg ctgtatctcc tgtttgatat ccccctgtat cagaagcgat ttgggctgtt
     5341 ccgttttttg ccgatacatt gtatccaggt acactatata caccataagc actaccgtag
     5401 ttttcgcttt cgacaacatc tctcatatat tcacggactt tttctgttgt ttcttttgaa
     5461 aaaggttgcc ctagaacttc tgtttgagtt gttcgctctt ctccagtttg gggatccacc
     5521 actttgctga tatatcgagg ttgaatcatt gtaccgttat tcgcaataga agtgaaagct
     5581 ttcatcattt ggaaattcgt cacacctacc gcttgtccat aagcactcat tgctcggtcg
     5641 acaatattta atgtaggtaa agcaccgttt acttcatcat ctaatccaga atgagtactt
     5701 tgtccaaatc ccagtttttg taaatagtta taccatcgtc cgcctaatcg ctgttccagt
     5761 atgaccatcc cgacgttact tgaccatgaa agagcttgac gcatggtcag cacacctttt
     5821 tcaccaaagt catggtcatt gatcgtagca tcttctactt ggatttttcc agattggaag
     5881 gtttcattct cgttgaattc tccttcttcg atagcagctg cagtagtgaa aactttcatg
     5941 gttgatcctg gttcataact atcttgtacc aagaaattac gccagatagc atcttttcca
     6001 gtcaatcctt ccattgtttc aggattgaaa gtaggtcgtt gtcccattgc caagatttct
     6061 ccggtttttg ctttcatcag gactgctgtc aattcttctg gctgatattc ttcatttact
     6121 tgatccatca gtgtttctaa ataactttgt agtctgctgt ctaaagtcgt gtagatatct
     6181 tgtccgtcga cagctttttc ttcttctgcc actgtacctg gcagaggatt ttgaaaatta
     6241 tctttttgat aaataatctt accatcttta ccgctcaaga tatcattgta agcagactcc
     6301 aaacctagtt taccaactaa gccattctca tctttgtctg gaactgcgta tccaataaaa
     6361 tgagaagaaa agacgccatt tggatacatc cgagcctgat ggtcgacaaa ataaagacca
     6421 gcgacacctt cttttttcat gtccgcttcg atgttttgct tggtttcttg ggagatattt
     6481 ttcccatagc taccaaattc aacttgatac agctcttttt ttgcgccgtc ctctaaaata
     6541 ttcagtgcat cttttttgtc tatagaaaga ttcttatgca ggatttctgc tattttatca
     6601 aaattctttt cttcaacata cagtttttta tccccagagg tatatgtttt tgagagtatc
     6661 gctttaattg aataagagga ggcatcttct gcgagcgcca caccgttacg atcataaatc
     6721 gttcctcttt ttgcttttac tacttcactt ccttggtaca actgttttgt tttttcagct
     6781 aatgaggtac cagcaacatg acctccaata acgatatacg agaaacggac ggcaaataaa
     6841 aagaacaatc caatactcgt agcaaataaa atgataccta cttttttgcg attgttcatc
     6901 ggatttagat ttttcttttc cataaaccgc cgaattttat ttttaagact catttacttc
     6961 actttcctta aattgtcgtc attaatagac aatccttttt cttctgcgat ttgtttaatc
     7021 cgttcagtac gagaaagttc gtttttttct tgttctaaac tcgttttttc ttgcttttta
     7081 tcattgattt ctgtttgtat tgacgtgatt tctttttcag ctccgctgat atttgtgcgc
     7141 agcataactg ttaaaacaca taaagcaaca atagcaatcg ccacgcttgc cacagcaatt
     7201 ttttccaaag cagaaatctg ctttaatcgt tttgccggtg attgtggaag aatgatttct
     7261 tgattcgaag aagaatcagg catttgttgt tccggctgat ccaccaattc aggtaaatca
     7321 tattcgtaat cttgcatttt ctttaattct gccatcattc tactcctctt tcattcttat
     7381 tttttctgca atacgtaact ttgcactgcg tgatcgattg ttttctgtta gctcttcctc
     7441 tgaaggaagg attggtttac gagtaatgac tttcagttct ggttggaatt cgtcaggtat
     7501 gacaggaagt cccggtggta gatcttgtac cgtactatat tctttaaaca tacttttgac
     7561 aattctgtct tccaatgaat ggaatgtgat tacgctgatt cttccattga ctttcaataa
     7621 ccggattgcc tgttctaaag aggcctcttc ggcaccgagt tcgtcattta ctgcaatccg
     7681 cacagcttga aaaattcttt ttgcaggatg cccgcctttt cttcttgcgg gagctgggat
     7741 gacttgttta atgatttcga caagctcacc agttgtttgg attggttttg tttttcgtac
     7801 tcgttcgatt tcacgagcaa tttgtttgga aaatttctcc tcgccatagc ggaagaaaat
     7861 ctttactaat tcatggtagg aatattcatt aattacgtga taagcggata aaggagctga
     7921 ctgatccatg cgcatatcca acggtgcatc ttgatgataa ctaaatccac gttctgcttc
     7981 atctagctga ggagaggaaa cccctagatc atacaaaatg ccgtctacct tatctacatg
     8041 ctcactcaag cgttcttcta attctctgaa gttcgctttg ataaatgtga ccataccttg
     8101 ttcgacataa gaagctaaac gcagcttggc gtgatcgatt gctttttgat cttggtcaaa
     8161 cgcatacaga tgccctttag aacttagttg gcttaataaa tattcactat gtccggcacc
     8221 gccgagtgta cagtctacat aaacgccatc cggtttgatg ttcaatccat ctaccgtttc
     8281 tttcaacatg actgtatagt gttgaaatgt ttcagtcatt agtcagcctc ttcctattct
     8341 tatagtccga aatcaatcat actttcagca atttcgtcaa agttctcttc tgctgctgca
     8401 gaaaaatctt gccatcgtgt ctcatcccaa atttcaattc ggttagatac accgatgact
     8461 acgcaacctt ttgtcaaagc tgcataattt cttaacgtgc tgggtatatt gattcgtcct
     8521 tgtttatcaa gttcgcattc agtggcagcc gaatagaaga atcgcacaaa agtacgtgcg
     8581 tcttttttag ccaacggcat ttcgttcagt ttagcttcca gattttccca ttcgctcatc
     8641 ggatagccga aaagacagcc atccaatccg cgtgtcagaa caaacttttc gccaagttct
     8701 tcacgaagtt ttgagggcac gattaaacgt ccttttgcat caatattatg ttggaattca
     8761 cccatcaaca ttgaaagccc cccattcctc cactaactaa aattagtcta ccacaatccc
     8821 ccactttcta ccaccttttc tgggaaatcc gggaacaact ttttttattc ataaacattt
     8881 ttttaaaaat caagctgcaa aaagcctttt aaaagacttt ttacagcttt tctatttttt
     8941 tcataggtaa gaaagtatgt tcacaatgat caagacagcg tacaagacga ttgtcaacag
     9001 aaaaaccaaa cgccaagtca tcttgaaata acgcggataa ataatttctt cataataata
     9061 tgcttggaaa acagcaatac aaattcccat tagcaaaaca gaaataaaga aaaacggtaa
     9121 gatagaaatt gtccctgtat ttctagacaa tgcatgcata ccgccccaca aaaaaggaat
     9181 cgccaaatca ggggctttta ttttaaaacg acgatttaac gaaaataatt ttacaagaaa
     9241 ttggcaacca aacaaaacga ttaccgggta aatataccaa aacaagataa gtggtgtgaa
     9301 tgtgcccatg aacgtaaact ccttttttat cgttgcttca agtatactgt atatacttta
     9361 cacaactgtt tcattttatt taaatttatg cagttatatt ctatatctta caaacacatt
     9421 ccatgtattc caattcccta tccgttataa taaaagaaac aggcaacagg aggaaaatca
     9481 tgatcaaata ttttacatta gataatgaga aactgcgtaa taccggaaag gaaactgacc
     9541 aaacgatctg gtattgtgta gaacgtccta atcaagaaga gattcatcag ctgacacatc
     9601 aatttgaaat tccgtcagat tacatcactt ccatattgga cgatgcagaa aattcccgta
     9661 cagaagaatt caatcaatca aagttgacaa agccagcgtt attattgtta caatatcctt
     9721 ttgctaaaac aagtcctagc ggttattttc aagtggatac atacccgctc tctattattt
     9781 tgacgcccaa aaagaagctt atcacaatta cgaatcatga accactgttt ttaaagaaag
     9841 tctttagcca gacctttcca aaaagtgata tttcgcctaa tctcaatatc ttctttcaat
     9901 tcatttggca ggtcacactc tcttacaaca gttatttagc tacgcttcaa tctcaatgcg
     9961 atactttgga agccaaactt caagtatcca ctgaaaacaa acaactttat caaatcatgg
    10021 atatccaaaa aagcctcgtt tatttcagag aagcgacttc tgctaattta gatgcattga
    10081 aagaactagc gcaaaataaa cttttccaag aaaatcatgc tttgaaaaat catttacgtg
    10141 atgtattggt ggaaacagag caggcaatga ctacttctcg cattcaatta aaactagtag
    10201 aacaaatgaa tcagactttt tcagcaatcg tatcgaataa tctgaataat attatgaaaa
    10261 ttttaacttc tttaacgatt attttaacta taccgacaat cgtcggcagt atctatggaa
    10321 tgaacattaa gttacctatt gctgaaaggg atgatgcctt tatatggcta gttctattaa
    10381 cattatttat ttgtatcgta actacctatt atttaaaaaa agggaagttt ttataaaaaa
    10441 gccctcttgt ggaaatggaa aaaagattgt acacttgaat caactaatta atgacgaggt
    10501 tgaaaacaat gcgtgaaaaa aaacaaacaa gtacttttgc taaagtaaca aaatttgtca
    10561 tttggacaat gctgatttta acgattggat ctgttgtatt gactgctgta atgagtgtca
    10621 tgtaaaaata aaatatagct atcagctgct gaaaaggacc tgaaccactg cgaggtggct
    10681 caggtccttt ttgcgagttt caatagttct tttgcatgtt cgagtgtgag ttttgtaatc
    10741 tcacttccag ccagcatacg tgcaatttct tcttctcttt cttttgtttt caactctgaa
    10801 acagaagttt gcgtacgtcc atcaatcact gctttcacaa tataatattg atagtcggcg
    10861 actgcagcaa cttgaggcag atgagtgatg cacaaaactt gagagtattt cgatatttga
    10921 gaaattttat ctgcgatggc ctgtgcaact cttccgctca cacctgtatc cacttcatca
    10981 aaaataatac ttgtcacacc ttgctcagaa gaaaaaatag ttttcaaagc aagcaacatc
    11041 cgagacagtt cgccaccaga agcgactttc accaaaggct tcagtggctc tcctggattg
    11101 gtcgtaatgt aaaattcgat ttggtcaaat ccatcatgat tcaattgtct attttcttct
    11161 tttaagaaac gtacctcaaa ctcagtgttt tccatataca gacttttcaa ttcatgcaga
    11221 atggattttt ccaattcttt tgcgattttt ttacgcgctt catgaagatc tgcggcttgt
    11281 tgctgaagta gaagctcttt ttgctctagt tcctctttca ttttgtccaa ttgaccttct
    11341 gaaaaatcag aagaagctag ctcttcggtg atttcgtcat agtaagccaa tattgactca
    11401 atggattcgc cgtatttacg tttcaactgt cggattaatt ctaaacgttg cgtaacttct
    11461 tctaaacgac tctcatctaa ctccaataaa tcaatctgac gactcatatc gccgattgca
    11521 tcttgcagca aatagtaagc actttggata ttttcataaa ttgcttcata ctccacatct
    11581 agatgggcga tcccttgcag ttctgaaacg gccaagccaa caccatctac actgttttgt
    11641 tcattattcg tcagtgctcc gtaacctgca gctaatccat cagcaatttt ttgataattg
    11701 ctaagttttt ctctttcttc ttttaatttc tcttcttctc cgacttgcaa atcagcagca
    11761 gctatttctt cttgctggaa atggagcata tctattctct gaacatagga tttttcattt
    11821 tgctgcactt ttcgtaccct tttttccagc tcacgataag agatatattc ttgctgataa
    11881 ctctttttct tctgttggaa agctacatct ccaaaacgat ccaataaagc aagatgtgat
    11941 tcaggctgga gcagttcctg atgttcgttt tgtccttgga tatctactaa ataactgccg
    12001 attcttctca gattagctaa tgtgataata tgaccattca ctcgacaaac attttttcca
    12061 gttaaggaca tatcccgtcg aacaatcaga ttgtcctcat ctgtttcgat ccctaattca
    12121 accatcagtt cagagaatcc ttcttgtttt ggtagttcaa acaacccttc aagcacacat
    12181 ttttctgctc cttgacgtat ataatcgctt gaacctcttc ctcctgctaa cagacctaaa
    12241 gcatcgataa taatggattt acccgcacca gtctctccgg tcagcgcggt cattccttcg
    12301 tgaaaagaaa gccgcaattc cggaataatc gcaaagttag taatactgat ttcttgtagc
    12361 atcacattcg ctcctcaact taaaggtaat gcaggaagtc tttcttcaat aaaactgcct
    12421 ggtcttcagt tcgtgtaatc atcaaaacat tatcgtcatc attgataatc gaaaataatt
    12481 ctttcttgta acgtttatca atcaaatttg ctgctgccga agcatttccc ggcaatgttt
    12541 tcaaaataac aaatttttcc atttggtcaa ctgccacgaa agcgtctttt aatagttttt
    12601 ccaatttagt atttacatct tcgtttgttt cagctggtat gctataacgg tagccgccat
    12661 ctgaagccgg taccttgatc agtttcagtt cctttatatc tctggaaatc gtagcttgag
    12721 taacagatat tcctttgctt ttcaggattt caacaaattc ttcctgcttt cggatatctt
    12781 gctcactcaa caatcgagta atcaaacgat gccgatcttt ttttctcatt acgtcttcgc
    12841 tccttagcat attcttctta atcaatgata gcttatttcc ttggtataat aaagaagaat
    12901 ccgcatactt ctcacttgat tttcacagtt acaatttatt tatggtcaaa ctgttcgtgt
    12961 gcatttgaaa ccacttctgc catattaata gctgaattga ttgttccgct ttcgggtact
    13021 ttcctcaagt gagccaagaa ctcgatattt ccttctcccc ccgtgatcgg agaataactt
    13081 aattccagca catcatatcc accttgtgcc gcaaatgaca aaatgtcttc taaaactttt
    13141 tgatgggtct gagggtcacg aacaattccg tttttcccta cagcttctct gcctgcttcg
    13201 aattggggtt tgattaaagc aatcacttct ccctcttctt ttaaaatagc atgtaatgga
    13261 ggcagcatca gttttagtga aataaaagaa acatcgataa cagcaacttc tggaattcct
    13321 aaggtaaagt cttcgggttt tgcataacga aaattcactc tttccattac gacgactcta
    13381 ggatcttgtc ttattttcca agctagctga ttgtagccaa cgtctaaagc ataactcatt
    13441 tttgcgccat tttgcaaagc tgcatcagta aatccgccag tggatgcacc aatatctaac
    13501 aatgtttttc cttcaacaga tagatcaaat gctttgagtg ctttttctaa ttttaatccc
    13561 cctcgagaaa cataaggaag ttttttacct ttgactctta attcactact cactggaatt
    13621 ttttctcccg gtttatcaaa ccgctcgttt ttttcgttgt aaatcaaacc agccatcact
    13681 gaacgtttgg cttgttctct tgtttcaaat aatccttgtt tgaccgctaa tacatctacg
    13741 cgttcttttt ccattctttc cctcatttct ccacatcaaa ttgcttgatc atctggcgca
    13801 tcagcccgcc atcaaaagaa aaaacgtcct cttctaattt gtctatgatc ttgttggcgg
    13861 ataacagctc aatctccaac gcctcacgtg tcttttctag gcctaataaa cgcgggtaag
    13921 tatttttttc catacgttca tctttaccag ctgttttccc taatgttttc gttgtaccaa
    13981 tcacatcgag tagatcatct cttatctgaa aagctaaacc taaatgttcg gctaatcgtt
    14041 gaagtagcaa taagatttct tcaggttgct tcgctaatat gccaccagcc agcaaggcat
    14101 aacgaatcaa atgccctgtc tttctttcat gaataaatgc taattcttct aaagaaagct
    14161 ttttcgattc gccctcgata tctgcagctt gcccagcaac cataccttgt gatcccgcac
    14221 aaacagctaa ttgctgaagc agcagaagct taggagaatt acctaagtgt gccatactga
    14281 ttagctggaa tgctcctgta agcaaaccat ctccggccaa tatagctaat gcttcgccga
    14341 atactttatg attggtaggt tttcctctac gaagatcatc attgtccatc gcaggcaaat
    14401 catcgtggat caaggaatac gtatgcacca tttctaaagc agctgctact tgatagacgg
    14461 gaacatcaat aggctcatca aaggaagcaa cggccgttaa aagtaataag ggacgtattc
    14521 ttttcccgcc tgctttcact gaataactca tcgcatcata gagtcctcta tccgttgtat
    14581 gctcatctaa aaaagacaag atttcttttt ctacagcagg tagatgaata gctgaaaagt
    14641 cagttaatct catctcagct ttcctcgttt ccgtcaaaat caacttcttc atttgattct
    14701 gtcatcattt tcgtcaaggt ctcttctgct ttagctaatg tatcttggca atgtttgctt
    14761 aattccattc ctcgtttaaa cgaatctaga gctgattcta atggaacgtc tccttgttct
    14821 aactgcatga caattttttc taattcttgc agtgactctt caaaagttgg attttccata
    14881 tttttattct cctttgtcta tctcttgtac ttgtccttta acgattccat cttgataatg
    14941 gatcgtcagc aaatcccctt gatgaatctc ggtgacactt tttactactt gatcatttaa
    15001 cgtcgtatag ctgtagcctc ttcccatgat tttcaatgga ctcaacaaat caagagattg
    15061 aacagcttga gcaaaacgct gctgctgttg tttcatatat tgttggattt tctcttctaa
    15121 gcgtttttct agataagcag tttcttggcg agctgcttta actcgataaa gcggagccac
    15181 ttgttccaat tggtggctta atgcgatcgt ttgtttttct ttcgcatgat aaatttgctg
    15241 tgtagcttga tataatcgct gtttcaactg gtctaatttg atggtctgac cttcatacaa
    15301 tctttcaggc tgccgaaata cataggaacg tcgagcacgc tcgaaccgtt cttgtttgcg
    15361 ctggatctgg cgtacaaacg cctgctgcaa tctagcttgc cgttcttgaa tgcgcatcaa
    15421 ctcttctgac aaaacaggaa cagctaattc tgcagctgct gttggagtgg cagcccgaac
    15481 atctgctact aaatcagcga tcgtggtatc tgtttcatgt cctacagatg aaatcaccgg
    15541 cgtattagct tgataaatcg cccgagcaac ccgctcttca ttaaacggcc ataaatcctc
    15601 aatcgaacca cccccacgac cgatgatcat tgtatcgaaa ttgcccatct cttctactct
    15661 ctggatattc ctgacaacat tgtccgctgc ttggtttcct tgtacaactg ttgggaacaa
    15721 aaccaattgg acaattggat atcttctttt caccgtcgtg atgatatcgc ggataaccgc
    15781 tccgcttgga ctggtaagga ccgcgattcg ctttgggaaa cgtggcagca tttttttagg
    15841 cccttgaaaa aggccttcct tcatcagctt ttcttttcgt tcttccaatt cttgatataa
    15901 agcacctatt ccatcaggct ccatgtgctc tacatagatt tgataagaac ctcccgcttc
    15961 atataatgaa atacgcccaa cgacaaggac tttcattcct tcttttggct ggaatcgtaa
    16021 tttttggaat gctcctttga acataatagc agatattttt gcatggtcat cttttaagct
    16081 gaaatattga tgggcattgg cacgtaaacg gaaattcgat atttcgcctg ttaaatatac
    16141 gcgttctaaa taaggatcgg catcaaattt tcttttcaaa tacttcgtta atgtagtgac
    16201 agttaaatat tcttgtgcca tctattcacc tgctccaaaa gcttcggcag attttacggt
    16261 ttgatacatc agcatcgtga tagtcattgg ccctacaccc tttggcacag gagtgatcca
    16321 gcctgcaata ggctccactt catcaaattt cacatcaccg atcagcttac cgttagcatc
    16381 acgattcatc cctacatcta tcactacagc acctggtttt acgaattctt ttgtgacaaa
    16441 atgtccacgt cctattgcta cgactaaaat atctgcttct tgtgccagtt cagccagatt
    16501 ttcggtttta gaatgagcaa cagtaacagt tgcatctgcc atcaagagta gctgagccat
    16561 cggtttaccg acgatattgc ttcgtccgat aactactgct cgtttacccg ccggatcgat
    16621 agaataagct tcaaacattt tcatgatgcc gtatggagta caaggaatct tgtccggttt
    16681 acccgcaagg agtctgccta aattcatcgg atgaaaaccg tctacgtctt tttcagggcg
    16741 gatagctaac aggatctttt cttcatctat atgctttggc aatggtaatt ggaccaatat
    16801 accatgaaaa ctaggatctt gattgtattt ttcaatttct gcaagtagtt cgtcttcaga
    16861 aatcgtctca ggataacgct ctaccaatga atggattccg atttcttttg ctacacgttc
    16921 tttatttctt acatagattt ggctggctgg attttcccct accaataaaa cgactaatcc
    16981 gggatggata ccattatctt tcaatttttg gattttttca gcaatctctg cctgcatttt
    17041 ttcagctaat tcttttccgt taatcaattc tgccatctaa ccactcccag atattttttg
    17101 tgttttcatt ctaccataga attttcccga tttataacag gtttatgatg attcatagaa
    17161 aaaagctagg acttacgccc cagcttctaa ttctttcaat acattggata aaacaccgtt
    17221 gacgaattta cgtgaacggt catcactaaa tgtttttgcc aactcgattg cttcatttaa
    17281 agcaacagcc gctggtacat ctgtcacata agtcatttca aaaatcgcga tacgcaagat
    17341 caccaaatcc atttttgaga tccgattgat gtgccaattg ttttttaaat gtttttcaat
    17401 cactttatcc aattcatctt tttttgcaca gacaccgcca actagcgtat caagataaac
    17461 aggaacaaac tctgattcat cttcgttgat catgtcgcgg tggtctaatt cgattgcgtt
    17521 gaaaatcgca tcttcttttg ttaggtctgc attgaagtct aatggaaata aagcctgcaa
    17581 tgccatttcc cgaatctcgt gtcttgataa ttctttattc attctcttcc tcttcgtctt
    17641 caaaaagttc ttcgattgtt ggttgttcta atttttcagg aacaacagct actacatgga
    17701 tattgacttc agataaatcg atatccgtca tgaaaaggac ttgctggctg acccgttcct
    17761 gcatttccat tgctacttta ggaactgaaa caccatattc tagataacag taaagatcta
    17821 ctttcaagcc atcttcctcg tttgctaagt aaacaccttt tccgtgagct gcacgtccta
    17881 ataattcagt gacattgctg gcaaaagttc cgcgcattcc gtatacgcct tctactttag
    17941 aagctgcaat cccgataatt acttcgatga cttcaggagc gatcacgatc tctcctaaat
    18001 cttggtttgc gtttaaaatc aaattttttt catctgccat ttatttattc ctccctttgc
    18061 ctaagcacga gatacatccg aattctctat gatgcggcat agtgatcacg tggtaagaaa
    18121 actgccgccg atcagctgca aactgctatg aattggagaa aacacccaac tttattatct
    18181 cttacagctt aaaagttctt ctatagtgta tcattaaatc gagtgatttt ctataacaaa
    18241 gtctgtgaac actgattttt acgcttactt cttctattga tgaaactata agatgatcaa
    18301 ttcttttggt gaacgagtca acacacgatt tccatcttct gtgatcaaca agtcatcctc
    18361 aatacgtact ccaccgattc ctggtaagta gatccctggt tcatcagtga tcacatttcc
    18421 tggtacaaat tgcttttctg cacgagaaga aacgtttggt ccttcgtgga tctctaaacc
    18481 gatcccatgt cctgtagaat gtccaaatgc ttccccgtaa ccatgttttg aaatataatc
    18541 tctagcaact gcatctaatt ggacaccagt cattcctggt tttgcagcat caataactgc
    18601 taattgagca tctaagacta tttgataaat ttcttttaat ttttccccag gatcaccaat
    18661 agcgaatgta cgtgtcatat cggaaacgta cccttcgtaa tagcaaccaa agtccaatgt
    18721 gatcaagtca ccttgttcga tgattttttt gcttgctaca ccatgcggca tagccgaacg
    18781 caatccgcta gcaacgatcg tatcaaaaga aacacctgaa gcgccaagag aacgcatata
    18841 aaagtctagt tgatttgcga cttcaatctc agtcatgccc ggttggatca ttttcaggat
    18901 gtgatcatat gccatatccg caatatgaca tgctttttcg ataatagcga tttcttcttc
    18961 atctttcact tctctgagtt cttcgatcat ctgtgaaaca ggtactaatt ccgcatcgat
    19021 gatttcttct aaaaccgaat attctaaaaa tgagactgtt gtttcctcaa aagctaatga
    19081 gtgcagttct tctttgcgta ctaagtttgc tacctcatca aaaataggtc cgacattttt
    19141 aataatttca aacccttgag cctgcgctgc cgcctgctcg gtataacgaa aatcagtaat
    19201 aaaaaatgct ttttccaaag taataacagc taatcctgtc gtacctgtaa agttcgttaa
    19261 gtaacgaaga ttgtacggac tggtaaccaa aaatgaatcc aaattttctt cttgcatttt
    19321 tttgcgcaat ttttctacac gtagcatcat aatgaaactc cctctttctt tgcacgcttt
    19381 tttcc
//
LOCUS       DAFFSV010000045        19009 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFFSV010000045 DAFFSV010000000
VERSION     DAFFSV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.045"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(107..1405)
                     /gene="eno"
                     /locus_tag="LR110_001448"
     CDS             complement(107..1405)
                     /gene="eno"
                     /locus_tag="LR110_001448"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBL4822415.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1521..2276)
                     /locus_tag="LR110_001449"
     CDS             complement(1521..2276)
                     /locus_tag="LR110_001449"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBL4822416.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2380..3570)
                     /locus_tag="LR110_001450"
     CDS             complement(2380..3570)
                     /locus_tag="LR110_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBL4822417.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3680..4681)
                     /gene="gap"
                     /locus_tag="LR110_001451"
     CDS             complement(3680..4681)
                     /gene="gap"
                     /locus_tag="LR110_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL4822418.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4722..5813)
                     /locus_tag="LR110_001452"
     CDS             complement(4722..5813)
                     /locus_tag="LR110_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBL4822419.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5962..7296)
                     /gene="rpoN"
                     /locus_tag="LR110_001453"
     CDS             complement(5962..7296)
                     /gene="rpoN"
                     /locus_tag="LR110_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBL4822420.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7356..7427
                     /locus_tag="LR110_001454"
     tRNA            7356..7427
                     /locus_tag="LR110_001454"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7389..7391,aa:Arg,seq:ccg)
     gene            complement(7619..8503)
                     /locus_tag="LR110_001455"
     CDS             complement(7619..8503)
                     /locus_tag="LR110_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL4822421.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(8590..9489)
                     /locus_tag="LR110_001456"
     CDS             complement(8590..9489)
                     /locus_tag="LR110_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBL4822422.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9506..10012)
                     /locus_tag="LR110_001457"
     CDS             complement(9506..10012)
                     /locus_tag="LR110_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBL4822423.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10201..10821)
                     /locus_tag="LR110_001458"
     CDS             complement(10201..10821)
                     /locus_tag="LR110_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBL4822424.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(10897..11334)
                     /locus_tag="LR110_001459"
     CDS             complement(10897..11334)
                     /locus_tag="LR110_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBL4822425.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11497..12327
                     /locus_tag="LR110_001460"
     CDS             11497..12327
                     /locus_tag="LR110_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBL4822426.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12311..13678
                     /locus_tag="LR110_001461"
     CDS             12311..13678
                     /locus_tag="LR110_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL4822427.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13697..14506
                     /gene="yidA"
                     /locus_tag="LR110_001462"
     CDS             13697..14506
                     /gene="yidA"
                     /locus_tag="LR110_001462"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBL4822428.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(14549..14992)
                     /locus_tag="LR110_001463"
     CDS             complement(14549..14992)
                     /locus_tag="LR110_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBL4822429.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            15126..15497
                     /locus_tag="LR110_001464"
     CDS             15126..15497
                     /locus_tag="LR110_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL4822430.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(15666..16448)
                     /locus_tag="LR110_001465"
     CDS             complement(15666..16448)
                     /locus_tag="LR110_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBL4822431.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16445..17416)
                     /locus_tag="LR110_001466"
     CDS             complement(16445..17416)
                     /locus_tag="LR110_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBL4822432.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            17508..18902
                     /locus_tag="LR110_001467"
     CDS             17508..18902
                     /locus_tag="LR110_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL4822433.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT         5373 a         3908 c         3382 g         6346 t
ORIGIN      
        1 tctctacctg ctgatatttc agcaagtaga gagttcgatt acttaactaa gtgatcatac
       61 tcgactatct ttcaatagtc gatcatgtat cttcattaga aaagaattat ttgtttttta
      121 agttgtagaa agatttcaaa cctttgtatt ctgcaacttc accaagttgg tcttcgatac
      181 gtagtaattg gttgtattta gcaatacggt cagtacgtga tagagaacca gttttgattt
      241 ggccagcatt tgttgcaaca gcgatatcag agattgttga atcttctgtt tcaccagaac
      301 ggtgagatac aactgctgtg tagccagctt ctttagccat ttcgatagct tcgaaagttt
      361 ctgttaatgt accgatttgg ttcactttga taaggattga gttagcaatt cctttttcga
      421 taccttcaga taatttttga gtgtttgtta cgaacaagtc gtcaccaact aattgaactt
      481 tgtcgcctaa tacgtcagtt aatttcttga atccgtccca gtcgttttcg tctaatccat
      541 cttcgattga gatgattgga tatttagaaa ctaattcttc gtagaatttg atcatttcgt
      601 cagttgtttt ttcgccttcg cctgagtcag ctagtacgta aacacctttt tctttgtcgt
      661 agaattctga agaagcagca tccatagcaa gaacaacgtc tttaccaggt acatagccag
      721 ctttttcgat tgcttcgatg attacttcaa aaccttcttc gtttgaacca aggtttggag
      781 caaatccacc ttcgtcacca acagaagttg ctaatccgcg agctttcaag attgaagcta
      841 atgcgtggaa tacttcagca cccatacgta gagcttcttt gaatgttgga gcgccaacag
      901 gcatgatcat gaattcttgg aagtcgattg agttatcagc atgtgatccg ccgttgatga
      961 tgttcatcat tggtgttggc aatacttttg tgttgaatcc gcctaggtag tggtataaag
     1021 gtacttctag gtagtcagca gctgcacgtg caacagcgat agaaacacct aaaatagcgt
     1081 tcgcacctaa tttacctttg ttaggagttc cgtctaaagc gatcatagct ttgtcgatag
     1141 ccatttggtc acgaacatcg tagccaatga ttgcttcagc aatgatgttg tttacgttgt
     1201 cgacagcttt agttacacct ttaccgccat aacgagcttt gtctccgtca cgtaattcaa
     1261 ctgcttcgta ttcaccagtt gaagcacctg aaggaaccat tccgcggcca aaagctccgc
     1321 tttctgtgta tacttctact tcgattgttg ggttaccacg tgagtctaag atctcgcgag
     1381 cgtaaacatc agtaataatt gacatgtttt gtctctcctt tgagtttgtt ttttcctaag
     1441 ggatttccct catatacgat tttagttaat tttgctcatt agtgcaaaca tttcttgcat
     1501 ttttcgcaaa ttttcaacaa ttattttaca gcgtctaaca atgacaaaaa tgaatctgct
     1561 tgcaagcttg cgccaccgac taaagcaccg tcaacatttt cttttgccat gtattcagca
     1621 atattttctg gttttacaga accgccgtat tggatacgaa ctgcttctga tacttctttt
     1681 ccataaagtt tttcaactgt ttgacgtaca actccacaaa tttcgtcagc gatttgtgcg
     1741 tcagctgatt taccagtacc gattgcccag attggttcat aagcaataac catgtttgac
     1801 acttgttcag ctgaaagatc agctaaacca gctgtaattt gtccttcgat ccattcagca
     1861 gttttgcctg cttcataggt ttctaaagat tcgccgcagc ataggattgg tgtcatacca
     1921 tttgcaaaga tagcttttgc ttttttattg atgtcttcgt ctgtttcatg gaaatattca
     1981 cgacgttctg aatgtccgat gatcacataa tgaacaccta gatcagcaag agctgctggt
     2041 gaagtttcac cagtgaacgc acctgagttt tcccagtagc agttttgtgc tgagattttt
     2101 agttctgtac cttcagctga tgtcattaat tcttgtaaga ataatgcagg agatccgatg
     2161 actgaatcaa ctttgtcatt tgaagggatt ttgtttttta ctgcttcagc aaattctttt
     2221 gcttctgatg cagttttgtt cattttccag ttacctgcga taataggttt acgcattgag
     2281 gaacacactc cttaatttat tgaaaaatgg ctagggagtc atgaaaactc ccttcacctt
     2341 ttgatcgatt actcgtcaga attgagcaaa cttggtcgtt tatttatcgt tgattgctgc
     2401 tagtcctggt aattctttac cttcaagcaa ttccaagctt gcgccaccac ctgttgagat
     2461 gtgtgtgaat ttgtcagcaa atcctaattg ttctgctgct gcagctgagt ctccgccacc
     2521 gatgattgtt gttgcgcctt ctaagttagc gattgcttca caaacaccga ttgtaccttt
     2581 agcaaagtta ctcatttcaa atacacccat aggtccgttc caaacaactg tttttgcacc
     2641 ttgtaatgta tcagcaaata attttactga tgctgggccg atgtctaaag ccatcaaacc
     2701 atcaggaatg tctccttcga ttacttgtgt ttcaacatca tttgaaaatt ctggtgcaca
     2761 gacattgtcg attggcaaga caagtttgtc gcctgctttt tcgattaatt cttttgctag
     2821 ttccacttta tcagcttcta ctagagagtt accgatcttg attccttttg cttcgtagaa
     2881 tgtgtaagtc attccgccac cgatcaagat tttatctgct ttaggaatca agttttcgat
     2941 tacgccgatt ttatcagaaa cttttgcgcc accaaggatt gcaatcattg gtcgttttgg
     3001 ttcttctact gcttcaccga tgaacttgat ttctttttcc atcaagaatc cagcaactgt
     3061 tgggatacca gttgaagcga tacctacgtt agaagcatgt gcacggtgag ctgtaccgaa
     3121 agcatcgtta acaaatacat cgcctaatga agcccagtat ttacctagtt ctgtatcatt
     3181 tttgctttct tttttaccgt caatatcttc aaatcgtgta ttttcgaata cgacaacgtc
     3241 gccgtcattc atgttgcgga ttgcttcttc taattgttct ccgcgagttt caggtacgaa
     3301 tgttacttct ttacctaata attcgcctag acgttttgca actggagcta aagattttcc
     3361 ttctttatct tcttctgttt ttacacgtcc aaggtgagag aaaaggattg cttttccgcc
     3421 ttgttctaga acgtagttga ttgttggcaa tgctgctttg atacgagtgt cgtcagtgat
     3481 cacgccgtct ttcaaaggaa cgttgaagtc aacacggaca agaacttttt tgtcttttaa
     3541 atcgatatct ttcactgttt tcttagccat tttatttcct cctgaataaa cttattatca
     3601 aaaaaagcgg aagaagcgcg attgcttccc cgcttttaaa tgtacatgtc tataatttac
     3661 acttgtacgt ttgtctatct tataagttag cgaagtattc taaagtacgt actaattgtg
     3721 cagtgtatga catttcgttg tcgtaccaag caacagtttt aactaattgt ttgtcgccaa
     3781 ctgtcattac tttagtttgt gtagcatcaa ataatgaacc gtaagtcata ccaacgatat
     3841 ctgaagaaac gatttcgtct gtgttgtatc cgtaagattc gtttgaagct tctttcattg
     3901 ctgcattgat ttcgtcaaca gttacttctt tttcaagaac tgttactaat tctgttaatg
     3961 aacctgttgg tacaggaaca cgttgagcag ctccgtctaa tttaccgttc aattcaggaa
     4021 ttactaaacc gatagcttta gcagcacctg ttgagttagg aacgatgttt gcagcagcag
     4081 cgcgtgcacg gcggaagtca cctttaggat gaggtccgtc tagagtcatt tggtcacctg
     4141 tgtaagcgtg gatagttgtc attaatcctt caacaacacc aaatttgtcg ttcaatgttt
     4201 tagccatagg agctaaacag ttagtagtac atgaagcacc tgagataact gtttctttac
     4261 cagttaatgt ttcgtggttt gtgttataaa cgattgttgg aacatcgttt ccgccaggag
     4321 cagagataac aacgcgttta gcaccagctg ttaaatgttt ttcagctgct gatttagaag
     4381 tgaagaaacc agtacattct agaacgatat ctacgcctag ttcgccccat ggtaattctt
     4441 ctgggttgcg gttagctaat actttaactt cttttccgtt tacgttgaat gaaccttcat
     4501 gaacttcaac tgttccgttg aaacgtcctt gagttgtgtc gtattttaac aagtgtgcca
     4561 acattttagc atctgtcaag tcgttgattg ctactacttc gattccatct acatcttgga
     4621 tacgacggaa tgctagacgt ccaatacgtc caaaaccatt aatacctact ttaactgtca
     4681 tttaagattt cctccttatg aaaatcaaaa aaatatttat tttaaagggt tacccctttt
     4741 aaaatctcat ttgcagcagc ttcatcagtg atgagccacg tttgttttgg tgcatttttc
     4801 atataagctc gaattgcttt agcttttgat tttccgcccg cgattgccat gatgatcggg
     4861 actttttcca aatcttgcaa ttctatgccg attctaggga ccttataaac gacttcacct
     4921 ttttcattga aaaagtagcc aaaagattcc gcaacagcat ttgcttgttt cagcatgact
     4981 aattcttttt cagtcatctt acgacgtgct gccatgtgta atgcacgccc aatactatga
     5041 ataacacaat tcgcttcact aatcaagttt aagacttctt ggatagatgg ctcattgagt
     5101 aaagaattgt aagttgccag acttaattgt tccggaacat aaagtgcacg gtggttaccg
     5161 tttgctttag cagccattct tgcactgaca gaatttgcct gtacagttat cgcttcacca
     5221 atcccgcctc ttgcagggac aaacaggtta tgccgtttct tattttccag attagacaat
     5281 tgttcggcta ccacagccat agtagttcct cccatcacag cgatgatatt ctcgccttct
     5341 ggaagtagtc ggtctagggc ttcgctcagc gcttcgccga atgcatcagc cactttggtc
     5401 tgctcttcgc tgtttcctga aacaacgata cagcgttggt taccaaaata ttctgccaac
     5461 tgttgttcgg tttgatgcgt acctaaaagc tgatccatga agttttcaag gctttgataa
     5521 acttcttcgc ccttagttgt cagcgtcata ccgctttttg aggtgtcgat caaattcaga
     5581 tttttcaaaa gatctgtttc tgttcgcaat actcgttctg tgatccccat actttccgat
     5641 aaacttcgac gtccaatcgg ctgcatccaa tagatatttc gtaaaatttt atagcgttct
     5701 tgcaacactt cgataatatc tggtgcaaca gattcaatca tttttatttc atccagcata
     5761 gcgatctcct tagttggaca ctttacgtcc aatgtagact taaaacgacc catatacgat
     5821 aaaaaaagaa attaactatt cgaatttcac atcaataaag ggaaatctca acctcacgac
     5881 tgtaagtcta acagttttgc tctcgttttt caactaaaaa gactagaaaa agtgaaatac
     5941 tttttctagt cttttgactg attagttatc ataacgtttt ctttttgatg aactcgggat
     6001 tcctaataaa tcacgatatt ttgctactgt tcgtcttgag atcgctatct catctttttt
     6061 cagcaattca accaatttct ggtcagacaa cggcttgttt ttgtcttctt gatcgactaa
     6121 ttcctgcagc tttttcttcg ctgtatctgc tgaaaggtct tctgtctgtt ctgtactatt
     6181 cgtaggaatt cgtgtagtga aaaatttttt taattcaaat acaccgaagt ctgtttccaa
     6241 atatttcccg ttgactgcac gactgaccgt ggattcatgc acatctatct cagttgcaac
     6301 ttcctttaac gtaagaggct tgattggacg ttccttgtcg ataaagaaag ctttttgccg
     6361 ggaaacaatg acttgagcaa cccgcaaaat tgtatcccca cgttgtagga tcgttttctt
     6421 caaccattcg aattcctgtt tccgttcttt taaataattt tcagtttctt tatctgcttg
     6481 ttgtgacatt tgtttgaagt aactctcttg aaagcgtata tttggctgat tttttcggtt
     6541 tgagataact tgtacatttt tatcgtcgtc cactaatacc cggacatcag gaataatgta
     6601 caattcgctg ctacggtcga aaatccggcc agggcttgga ctcaacgttt gtaaatagtc
     6661 gaatatctgc tgaacagcat gtagatcaac agaaaaacgt tgtgcgattt ctttccattt
     6721 tcgctccacc agttcctcaa aacattcttc caacacgata taagctaatt ccggcgcata
     6781 atcatctcgt tctgtttgaa gcatgagaca ttcttgcaaa ctacgtgcac ctactccagc
     6841 tggttctaat tgctgaatca gagtcaacgc atcgagcatt tggataggcg tagctccaat
     6901 ctcttttccg gcttcttcta aactaatcgt caaaaatccg ttcaaatcga tgtattctac
     6961 aagataaaga acgattttcc tcaaaaaagt atctcggtaa ttcaaatgga cttgttcgat
     7021 caatgaatca aataaagacc cttttgtatc aggaatctga tttaggtaat ttgtctcttc
     7081 tccttcataa gaagatgaag aagcttttat gtaatctggc tgccattccg gctcaacaac
     7141 ttccaccaat ggattttcca ttgcttgatt ctcaacgaat gccaacagct cctcactgtt
     7201 atattgtaaa acttggatgg actgttgcaa ctgttgcgtc atggctagtt tctgtgtctg
     7261 ctgctgtttt tgtgaaaaat tttgctcaaa tttcattttt ttgtccaatc cccttgtaat
     7321 tttttatctg tttagttaca attatcccag tgcttgcgtc cttagtgtag tggatatcac
     7381 gtaagattcc ggttcttgag acgggggttc gattccctca ggacgcgttc tactgctatt
     7441 ttagcataat ttacgaatag ttgcttcttt atttttaagt gaaaactgtc cgccaaagaa
     7501 aattggtgga cagttttttg ttgtacctgt atagatattg ttaagccttt gcaagtaagg
     7561 tagttatgtg ttcaactact tattaaaatg tgttatttat taaagatact tgtctcactt
     7621 actgaactgg ccgatgatcg tttctgccat aatagttctc gacagccgtt ggatgcccta
     7681 ataaatcccc aactgtcgtg atgcgatacc cttgctcttt cagataatcg atcacttgag
     7741 gaacggcacg aatcgtttca ggatagatat catggaataa aataattgcc ccatcgtata
     7801 ctgttgcttg gatcttctgc aaaatcaaat caggatttct tgtcttccaa tcttctgaat
     7861 caacagacca ttcgatgatc ggtcgctgga tgatcgttgc tcctacattc gtgacagaac
     7921 catagggcgg acgaacaaaa gtgggggttt tgccagtgac ttttttgatc tcagtgtctg
     7981 ctttcgtcac ttcctccgtc atcgactggg cagaaagagt ggccagattc tgatgatcgt
     8041 atgtgtgatt gccaatctcg tgccctgctt cagctgtctg cttcacgatt tcaggatgct
     8101 gcgccgtatt ttctcctaag acgaaaaaag tggcatgcac gtttttttct tttaagattc
     8161 gcagaagctg cggtgttgtg gtcgctcctg gtccatcatc aaagctgatt gcgacgcttt
     8221 tgacagccgg atctcggctc gtttttgtac tgtcttgtgt tgattgtgat gactgacttt
     8281 gtaccttttc cgctgattct tgaatggctt ctttgacgtt agctgtttca gttgattggc
     8341 ttttaatggt tgattgttct gattgtttga aagattgtga cgaatgctcc gaagagtgtg
     8401 tttgtccttt tacttcggtg atcaaggagg ccaccgaagt aatttcgaaa aacaaaagca
     8461 cggataaagc aataatactg ataactaaat aatttctttt cactatatct cttctttctc
     8521 tttttctctt cttattattt acttgtactt acttgaaaat tgaaactgct agtcgcaact
     8581 acttctcgtc taatgatcca aataatcttc cgatgccaaa atcgtttttg tcacggactt
     8641 aggaatgaca aattctggtg tacccatata accaggagca atttcgtatt gagaaaaagt
     8701 caaaactaat tgatgctctt tgttcaaata aaaacttcgt gtaggcgtaa caagtgttgg
     8761 tttttctatc gtcccttctt gaatatcttg ctcagtccaa tagtacttag atggatcttc
     8821 cttgacttgt tcggccatct gccgtttgac ttcttttgtc aaaacagata gataggtgtc
     8881 atctttgaat aaaagtggaa gtgagagctg gacactgttt ttcttgtcga ttgtgtcgta
     8941 tcttttttca acatggctat ctgctgctgt ttgggtgatc tgcctttcaa tcacgagaaa
     9001 ccggttatcg tctacgattt tctgataatc tcccaaaact tggtaatgat ccgtttcaaa
     9061 atttccatat tctttttcag ctttttcaaa atctgcttgt ccttcgcgaa agtatttttg
     9121 atttaatcca tgaattgttt tattttcttt ctcattttgt gtattgatct tcggtacctg
     9181 aatatcgatt ttttcagatc gatcagtaaa gcgttcgcct aagatcaatt caatgactga
     9241 tcctaagatg ggtaaatctt ctgcaaagct tcgaacttga ctattaaaaa gaaggctact
     9301 tgttgtaatc attacgatag ccaaagatac tccgaaccct tggagacgtc tctttctccg
     9361 ctttgctttt ataaatgatt gctcttcgat aataaaacgc tggcggatct cctctttaag
     9421 ccctttggga attgcttgtt ctttataagt tttctttagt ttttttaaat tttctttttg
     9481 ggaatacatg gcactcttct ctccttcatt ctaggatttg acgtaaagca ttcaatgagc
     9541 ggtacatttt cgttttaact gtgttttcat tcatatccaa gatagtcgca atttctttca
     9601 aacgaaatcc ttcaaaaaag tagaggatca gaatctcttt ttccggactg tctattttat
     9661 ctaaaatcat atggagctct gcaacggaag tttctaaagg taactgttgg gggatatctt
     9721 gaatttcaaa taagtccaca ggcgtccttt tttgtctgcg ccaactatct aatgccgtgc
     9781 ggatcaatat ccgataaaac catgcactaa aatgctcgat ctctttttct ttttcaaaat
     9841 agttcagggc tttatgaaaa ctatcttgca tcacatctaa ggcctcttct ttatcatgta
     9901 cgtaattcat cgtgatacgg aacatttttt gataatcagt ttcgatgagt ccattcagca
     9961 ggtgacggta tttttcgatc tgccgttttt tcttatatcc agtaaaaatc attctccact
    10021 cctttctgcc tcacttaata tgacgtacaa ggtgcgctaa aagtttcaat ttttttattt
    10081 tttatgtatt tacttgggat ttttatatac aaaaaagaag gtgtgacaaa aatcgtgtca
    10141 cacctcctta ttctgatagg ctgatcgtct ctgtcacaac ctctcgtaag aaaataatgt
    10201 ttattctttt tcagagtcag aaaattcttc ctcttcataa tcttcatcat catcgtcttc
    10261 gtctatgata cttaaatctt cttcgatatt tcctggcaag tcttcttcat catctgtcgg
    10321 gtcatctgct ccgatttctt gcaagtcaga attataagca gcgatttctt catcttcatc
    10381 atcatcgtca tcaaacagat catcgtcatc ttcttctgtc agatctgtat cttctgggtc
    10441 atcgtcattg taatcgatcg cgtcttcatc atcattgata aaggcgttga ctttcttgcg
    10501 tttacggcga cgtggagtat cttcatcctc ttcgtccatt ccatggttta cttcttcatc
    10561 gatcgaatcg atagcatacc atgaacgcaa gccccaacga ttatccccta atgagataaa
    10621 gctgccgtca atattcaagt cagtataaaa ttgtgcgagt gaatcacgaa tttcactgtc
    10681 tgatttccca agatagtttt ggatctggtt agctaaatca gaaaaatcca tgacatcgcc
    10741 acgttgttct aagatcgcgt gtgctacttc gatcattgat aattcgcttt tgtttgcacc
    10801 ttcaaataca ctaatttcca atcaggacac gtcctttcac agtctactcc ttatcataca
    10861 aaatcagaca caaaaaatca aacttttctt ttatatttac gctgtaaaat ccaaggtcaa
    10921 ataatattcg ccgatttttt ccggtcccat gaacagatca taatcgattc gaaccttccc
    10981 tgctgtggga cggtctttca aactaacatg cagttctttc gtaaaagtag caatctggat
    11041 cataccgtat ggtgttcgat aggatgtttc taaccgttct ttatagccaa atttcaaacg
    11101 catccgcatt tctcctgcac ggatcagctg tacatgacca tctggaacta atttgatcgt
    11161 tacaggaatc tcatcgcccg tctcctcctg aatctcttta taacgtatgt acagcgtatc
    11221 gccaattgtc accatttgcc ctttcaaatc aaaataaaaa tcttgcactt ccccttgctg
    11281 ttttactttt gtacgcaatt tgattgaaat cggtgttcct tgtgataatt ccattattca
    11341 ttctccttta cttctcatag ctacattgtt cctagtttct tttgttttgt caaccaactt
    11401 gtccattttt acagaaaacc ttttttccaa aacaactctt tgattttaga gtataatatt
    11461 gtagaaaata tgaatttata caaaggagcc ggttgaatga accctgcgat tcgactagat
    11521 cagcacattt atcaaaaaaa cgacttttcc ccttttgcat taacagatat attaacggaa
    11581 tattctaaag aacaccagca aatcttttta catttctggc aatatgactg cacagtcatc
    11641 ttaggtatga aggatgttcg aactcctttt ctaagtgacg ggatccaaac actggaagat
    11701 gcaggttacg ctccagtgat cagaaattca ggtggattag ggatcgtatc agatgaagga
    11761 attttgaata tttctttgat tttcccccaa agtgacacaa aaaaaataac catagatgac
    11821 gcttacgagc aaatgctcct attgactaaa tacgcttttc caaaggcacc gatacaagca
    11881 ttcgaaatca cccattctta ttgtcctgga acttatgatt taagcatcca tggcaaaaaa
    11941 tttgccggta tcgctcagcg tcggatcaaa aatgggattt ctgttatgat gtacttaagt
    12001 gtcaacggta atcaggaaaa acgtgggcaa gtcatgaagc gattttatca atctgcttta
    12061 aaagaagact ttggtacaaa tggctatcct gcagtggatc ctgctacaat ggcaacactt
    12121 gaagaactgc ttgaccaacc cctttttgta aaagaagtca aagaagcatt tgcaaaagct
    12181 tttgaaaagc tgtattctgc aagtgattta caggattcta ccgactggct aaaaaaagaa
    12241 atccagctgg agcaatggaa caaccaaatc cagagaatgg aacaacgtaa taacataaag
    12301 gagctacatt atgacaaccc cttataaata tcaaatgctg cctatggaaa aagtttttcg
    12361 tgatccagtc cataactata tccatgtcca gcatcaagta atcttagatt tgatcaattc
    12421 gaaagaagtc caacgtctac gacgcattaa acaattaggc acttcttctt tcacttttca
    12481 cggagcagaa catagccgtt ttactcattc attaggcgtt tatgagattt caagacgaat
    12541 ctgcgatatc ttttctcgaa atttttcaaa agaaaaaata ggcaacggag gctgggatga
    12601 tcaggaacgg ctggtgactt tatgtgcggc actgttgcat gatgtcggtc atggtcctta
    12661 ctctcataca tttgaacata tcttccatac gaatcatgaa gccatcactg tagagatcat
    12721 cacttctcca gaaacagaag tgtatcagat tcttaaccga gtagaagaag gttttcctga
    12781 aaaggtagct agtgtgatcc aaaaaactta ccccaaccca caagttgtcc aaatgatttc
    12841 tagtcagatc gatgcagatc gaatggacta tttgcttcgg gatgcttatt tcactggaac
    12901 agaatatgga acatttgatc ttacccgaat attacgtgtg attcgtcctt atgaaggcgg
    12961 gatcgctttt tccatgagtg gcatgcatgc agtggaagat tatatcgtta gccgatatca
    13021 aatgtatgtg caagtgtact tccatcctgt ttcccgtggt atggaagtaa ttttggagca
    13081 tctgcttcat cgcgcacacg agttgtatca agaagatgcc agcagttttg aactccattc
    13141 tcaattactt ctgccatttc ttgacggaag ctttacttta gaagaatacc tcaaacttga
    13201 tgatggggtc ttgggcactt actttactca atggtctgaa tctgctgatc ctattttaaa
    13261 tgatttagct aaacgttttt tgaatcgtaa accactgaaa tctgctactt tttctggcaa
    13321 ccgggattca aaattagtcc aagaactcac tttgttagta gaaaaggtcg gatataatcc
    13381 tgtttattac actgctgtga attctagcta cgatctacct tatgattttt atcgcccaga
    13441 acaaggcaga catcgcacac aaatcgagat tttgcgaaat gacggaactt tgatagaatt
    13501 atcacaggtt agtcagcttg ttgctgcatt agcgggacaa gaacaaggag atgagcgatt
    13561 tttctttcca aaagaaatgg ttgatccgtc cttgcgtgat cattatgatt tatttgacga
    13621 aacttatcaa gaatttgcca gccatatccg taatggcgca ttgattgaaa taaattaaaa
    13681 tatattggag gaaattatgt ctatcaaact agtcgctatc gacatcgatg gtactttatt
    13741 aaatgagaaa agagaagtaa caaaagaagt aaaagaagcg attgccgctg ctgttgcaaa
    13801 aggtgtttcg atcgttttat gcactggccg tcccttacca ggtgtccagg aacagttaaa
    13861 cgagttgaac ctttttcaag ataatgatta cgttattact tataacgggg cactcgtaca
    13921 acaaacgaaa tcaggtaaaa tcatcgcccg tcacggactg acacatgaag atttcctaga
    13981 gatcgaagta atggcacgcc gtgtcggcag tcatctccat tcgatcgatg acaaagcgat
    14041 ctacacgcct aatcgagata tcagcgctta tacgatccac gaagcaagtt tagtcaagat
    14101 gccgctaaaa tatcgaacac ctgaagaaat gacaccagac atgaacatcg tcaaaatgat
    14161 gatgatcgac gaaccagaaa ttttagatgc agcaattgcg cgtttaccgc aaactttccg
    14221 tgataaatac acaacggtca aaagtgcgcc ctattacttc gaagtactaa ataaagaagc
    14281 aagtaaaggt gcggcagttg cgaatctcgc acagcacctt ggtattgatc aagatgaaat
    14341 catggctatc ggagataacg agaacgactt atccatgatt gaatatgctg gtctaggagt
    14401 agcaatggga aatgccgttt ctcttgtcaa agaagcagca aatgtcatca cttcatcaaa
    14461 cgacgagcac ggtgtagctg aagcaatcaa aaaatatgta ctgtaataaa taaaaaaact
    14521 gagagaaagc tgatctactc gctttctttc agtttttatt tttttcgatc catctctcga
    14581 ctgcccatcg gtggctacca cgtttcaatt tcacaagcgc ttcttgaggg ctcacccacc
    14641 aaattttatt ggtcttttca gttggttctc ctatacgctt ccaagaatca gcaacaaaga
    14701 aatatcctgg attataataa taagttgcac gataattcga gtaaaaatac tcatcagctt
    14761 ctcccagata agaagaaatt tctgccacaa ttcccatttc ttctatcagc tcacggacaa
    14821 tcgcttcttc tttcgtctct tctccttcaa tttccccgcc aggaagaaaa aaagcaccat
    14881 ttggagcctc gattactgca atctgctgat ctctttctat gacaatatat gctgcataac
    14941 gggtttgata atcagcaaat tgcttttttt caccaaaaat tggtttatcc atgaatagtt
    15001 tccaccttaa cagttttatt ttctatccat tttacatgag aaatttctca tgaacaagca
    15061 caaatcaact atttccttac ttctttttgt tatgattgag taaaaggaaa aggagagatg
    15121 aatctatgaa aatggctcac acatgtgttc gagtgaaaga tcttgaagca tcaatcgaat
    15181 tttatcaaaa ggcatttggt tttgaagaaa gccgccgccg tgattttcca gaaaacaaat
    15241 ttacacttgt ttatttgaca cttcctgggg atgactatga attagagttg acttacaact
    15301 atgatcatgg tgcgtatgat ctaggagacg gctatggtca tatcgctata gcagctgatg
    15361 atctagaaaa attacacgag caacataaag ctgctggtct tgagattact gacttaaaag
    15421 gattacctgg cactgcgcct tcttactact ttgtcattga ccctgatggt tataaaatcg
    15481 aagtcatccg aggatagtta gtatggggca ttttactctt gattgaataa aaaacaaaca
    15541 ccaataatta aaatgagacg caaacttttc attaaattga aaaagtttgc gtcttttttt
    15601 aacaaccatt gtagctataa ggttgccact ctccaccaat ttatttttaa ttcatttgaa
    15661 agaaatcaaa taaacgaggt acctttagaa aacaaaactg cttttcctcc tattatcaca
    15721 cgatcttctt ttaattcaca ttctagatcc ccgccccttg atgacatttg ataagctgac
    15781 aattttgttt tattcattct ttctgcccaa taaggaataa gattagaatg tgctgatcca
    15841 catactggat cttcattgat tgttaatttt ggaaagaagg ttctagaaac aaagtctttt
    15901 tgcacacttt ctgctgtcac gatgactcct attcctaatt caaaattttt gatagctgta
    15961 aaatctggct gcaatttagc caccgttttt tcatcttcta atacgaaaaa taaatcacgt
    16021 gctaagtagg cttctttaat cttagcccca atcgcttctt catattcaga caatatcggc
    16081 acttttttcg gcataatact aggaaaatcc atgtaataat aatctttaat tcttgtcaca
    16141 aatagattac cgctttgagt agaaaattta atcgtttcat caggtatttt gtaatagtta
    16201 aataaaacaa atgctgttgc taatgtcgcg tgaccacaaa gatcgatttc tcgatctggc
    16261 gtaaaccatc taagctcaaa ttcttggttt ttcttcactg taaatgctgt ttcagataaa
    16321 ttgttttcaa tggcaatttt ttgcatcgta ccttctggca gccattcttc tagcacataa
    16381 accgctgctg gatttccttt gaacacctca tctgcaaatg catcaactac gtaataatct
    16441 aagttcattt taaagcccct ttctctaatt tgttataaat atattctgca caacaaagat
    16501 cttcaatagc tagaccaact gcgtcgaaaa tcgtcggcct cgattctcct ttataaaatg
    16561 tttttttctc agaaactaat tcgcctaatg taccaacaat acaagattcc tctaaatctc
    16621 cttctgccat ggctataagc aggtccccgc tttctgttaa ggcagctgag taatcatcta
    16681 catagatttg agaagtttcc atcaacgatg tacttatctc tcttgtatca ggagtaaatg
    16741 tgcccacagc attgatatgt gcgtcttccc ttacccactc tttttgaaga aatgcctctt
    16801 tactagaggt caacgtacaa atgatttccg ctggacgtac tgcatcttct actgatattg
    16861 catttataaa cgttatttta ggatactgtt ttttagcttt agcgataaaa gtatttcgtc
    16921 tttcttctaa aatatcgtaa acaaacactg tagtgatggg acgaatggta gaaatcgcag
    16981 tcaaatgcga caatgcttgt tgaccagctc caattaaagc aagtgtttct gcatgcggat
    17041 ttgctaagta atctgttgcc agcgcagata cagcagctgt tcgaatccag gtgactgcat
    17101 ttgcgtttat caatgctttg ggtagtccgt tttgagcatc aaacaagaaa atctctccta
    17161 aatgagaagg taaatgatac tgatgattat ccggaaaagc ggtaatgact tttgtgccaa
    17221 aatatctgtt ctcccccaaa taagcaggca tcatcgcaaa catatttttg ccttttccgt
    17281 caggtaaaat ttccaccaat cgaggatttt ggctgatttt accactttca taatcaaaaa
    17341 aacatttttt cattacttga atcgcttctt caaaactaaa agaagctact acttcctcaa
    17401 aattaataat tttcatagac aattccctcc tcacaaaaga ttaacttcta aaatcaatac
    17461 aatcaatggt aaaattgtat ggtatacaat tttaaggagt ggagaaaatg cctattaatt
    17521 cttatgacaa ttacccgtta acttggcgac cagacaaaca aaaattgaag cgtccttatt
    17581 atcgatcgtt gattcaacaa ttagaacaag atattttaaa gaataaatta cagaaaaaca
    17641 ctcagttacc ctcccaaaga gagctagctg attatttaga tttaaatttt actaccgttg
    17701 gcaaggctta taaatatgga attgagaaag gtttgctgta tacgaatatt ggaagtggca
    17761 catttatttc tcccaatgtc tttgagtcca tcaccatctc taccaatgaa gtaaccgaac
    17821 atatcattga ttttggtctg gttagttctt ttgaagaatg taataaattc gttgtcccgt
    17881 ttattcaatc aactagtaat aacccaaatc ttgttggttt gcttaattac caagatccca
    17941 aaggcacaaa ttttcaattg actactgcag caaattggtt aaaaactcaa ggagtcattc
    18001 cgtccttaga atctctatct attgtttccg gtgttcaaaa tggattggct atcacacttt
    18061 ctgcactttt ctctccaggt gatagaatag caacagaccg atatacatac gctaatttca
    18121 ttgaattggc tcagttatat catttagaga tcgttccaat tgattttgac gagcaaggaa
    18181 tgcgacctga tatattgatt caggaatgta gaaaaaagaa gattcatggt gtctttctta
    18241 tgccttcttg taataatcct atagggtttc aaatctctca caaaagacga gagcaattag
    18301 ccgaagtgat tgaaaaagaa ggtctatggg ttattgaaga tgatatccat tcatttttaa
    18361 caacttacta tcaacatacg atcattcctc ctttccaaaa tttattacct aaccaaacaa
    18421 tctacttagc tggaatgaca aaatttattt gttctggatt gcgagttgct tatcttgtag
    18481 ttccagtaca tgtccaaagc aagatagaac aagcaatttt taatataaat gtgaaaactt
    18541 ctggattaga tgtggaagtc atcactcaaa tcctccattc aaatgaagca aataggatct
    18601 tagaaactaa attaagtctt acaaaaagag ccaatcagct ttttgattct cttttcaaag
    18661 tagaaaaacc aacgaatcgt gtcccttact atcgaattct tccagttgat cctgagattg
    18721 accctaagat aattgagaat gactttcttg ctaacggtat ccgactttat cattccagac
    18781 gctttactac acgtgcgcaa ccagatgcat tcgttcgaat ttccctatcc tctaataacc
    18841 ttgaaatttt agcttctgga ctaagtatcg taaaagaaaa aataaaaaaa tatgaattat
    18901 aacctattga taaataaagg aatcaaatct tattgattcc tttcaataaa aaagaggcac
    18961 tacccgaggt tcaacaacgg gtagtgcctc aaaaaataaa cgaacattt
//
LOCUS       DAFFSV010000046        18632 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFFSV010000046 DAFFSV010000000
VERSION     DAFFSV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.046"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(335..1627)
                     /locus_tag="LR110_001468"
     CDS             complement(335..1627)
                     /locus_tag="LR110_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL4822434.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1624..2247)
                     /locus_tag="LR110_001469"
     CDS             complement(1624..2247)
                     /locus_tag="LR110_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822435.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2250..3026)
                     /locus_tag="LR110_001470"
     CDS             complement(2250..3026)
                     /locus_tag="LR110_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4822436.1"
                     /translation="MIYPVLWMIAGSFKTDQEIMSGSLNLIPETFQWENYAKGWAGFA
                     GISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDFKGKRFWFVVMLVTMMIPAQV
                     ILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQFMVSIPKELDESAIIDGCSK
                     YSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYLNKPRSYTVSIAIKLFADAGS
                     STTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGLKG"
     gene            complement(3098..3985)
                     /locus_tag="LR110_001471"
     CDS             complement(3098..3985)
                     /locus_tag="LR110_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4822437.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(4001..5284)
                     /locus_tag="LR110_001472"
     CDS             complement(4001..5284)
                     /locus_tag="LR110_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4822438.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(5967..6650)
                     /locus_tag="LR110_001473"
     CDS             complement(5967..6650)
                     /locus_tag="LR110_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL4822439.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6668..7285)
                     /locus_tag="LR110_001474"
     CDS             complement(6668..7285)
                     /locus_tag="LR110_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL4822440.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7295..8119)
                     /locus_tag="LR110_001475"
     CDS             complement(7295..8119)
                     /locus_tag="LR110_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBL4822441.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(8112..8672)
                     /locus_tag="LR110_001476"
     CDS             complement(8112..8672)
                     /locus_tag="LR110_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822442.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8846..9802
                     /locus_tag="LR110_001477"
     CDS             8846..9802
                     /locus_tag="LR110_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL4822443.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(10107..11063)
                     /locus_tag="LR110_001478"
     CDS             complement(10107..11063)
                     /locus_tag="LR110_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBL4822444.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11167..11769
                     /locus_tag="LR110_001479"
     CDS             11167..11769
                     /locus_tag="LR110_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL4822445.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11857..13356)
                     /locus_tag="LR110_001480"
     CDS             complement(11857..13356)
                     /locus_tag="LR110_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBL4822446.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13372..13896)
                     /locus_tag="LR110_001481"
     CDS             complement(13372..13896)
                     /locus_tag="LR110_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBL4822447.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14194..14943
                     /locus_tag="LR110_001482"
     CDS             14194..14943
                     /locus_tag="LR110_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL4822448.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14934..15719
                     /locus_tag="LR110_001483"
     CDS             14934..15719
                     /locus_tag="LR110_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBL4822449.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15688..16404
                     /locus_tag="LR110_001484"
     CDS             15688..16404
                     /locus_tag="LR110_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL4822450.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(16506..17540)
                     /locus_tag="LR110_001485"
     CDS             complement(16506..17540)
                     /locus_tag="LR110_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBL4822451.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(17544..18602)
                     /locus_tag="LR110_001486"
     CDS             complement(17544..18602)
                     /locus_tag="LR110_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBL4822452.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         5575 a         3924 c         3271 g         5862 t
ORIGIN      
        1 actgttttgc acgtgaaggc agtttgccat atttgaataa aaaatgttgt ttcaaatatt
       61 gaaaacagtc taacactact ttctgaaact gacaacgtgg tttacttatt tttttgtaaa
      121 atctaatgat ttttaatgca ttattccttt ttgttcgggt ttaaatagtt atttttattt
      181 tctcctatct aaaaaagcag cccacataaa aagaaggcat gacaaaaagc atgtcatagc
      241 ccctttttcc gaataaatag tgttcaaaag acagcctatc ggcaataagg caagatttta
      301 taaactacgg gaagtaattt ccgtaaatct attcttattc ttttaagctg acaagctcgt
      361 caatcactac tgcttttcct gaagcaatag aatgatttgc tgcgattcct gtcaaaatcg
      421 acaaaatacc atctacgtga gttgctgccc gattccacgg atcttcttct ttttcttcac
      481 caaatagatc atctaataaa cgcggatctc ctccgccatg tccgcccgct tcttgttcga
      541 cgattacctc atatggctca ccgaacatcg gatagacatg gatggactga agaacggctg
      601 ctccttcatc ctttttatct ccacctgcat tgatgtaaga cttttcgatc actttcattt
      661 cgatccgtcc tttgctgcca ttgaaagaaa cattgaatcc ttcccacggc atatacgcgt
      721 ttagcgaata cgtcaaaatc gcaccagttt catatttgac cgttacgcct agtgtatctt
      781 caatagagat atcatcggaa aatacactgc ggtcacgaat atatccactg tctttttctg
      841 cttctaaata catttctcgc aattcctcat tggctgacag gtctatagca aatggatcag
      901 ctttagctag ttcgcttcca tagcatcgac tgtaaaatat ttctactccg cgtttctccg
      961 cattatgcat cccataaaag tttaatccac cgaatgcaaa taccttttgg ggacggctgt
     1021 tcaaccaaaa attcactaga tcaaaatgat gggtagattt atgaacaaga agcccgccgc
     1081 tattgtgctt atttcgatgc catcttctga aataatctgc cccgtgttgc gtatttaaca
     1141 gccattcgaa gtggatagaa aacacatcac caatcacatc actttctatc agttccttta
     1201 tcttcgtgtg atacggtgca taacgataat taaaagtgac tcgcaggctt cttcccgtct
     1261 gtttttggac cgttattatt tcttgagctt tttcctcatc gatagtcaga ggtttttcgc
     1321 aaataacatc atatcctttt ttcattgcac gaatgatata atcatgatgc gttcgatcta
     1381 ctgacgtaat aatgacttca gccggccgta cgtcatccag tagttgctca aattgtgatg
     1441 gcatacatgt ctttactttt ttctgtccat acttgttcac aagtatccga tttgcataat
     1501 ccatccttgt ttgagataca tcacaaaaac cgactagttc acaagtttga tgatacgtag
     1561 aggcaattgc ctcataaaaa aaacgcgctc tcccaccaag tccgacttgt acatattttt
     1621 tcttcattta aagtcattct ccttttcttt taagcaaaat actgtgacaa atggcagccc
     1681 tggatacagt aatgcagtga gtaaaaaatc atgctgtata caccagaaca gtccatacaa
     1741 aaggcataaa taccacagaa accccttgac gttttttgac aacagccaga tattcctttt
     1801 gatcaaataa acaagactat ctgctttatc ttctttgcag atatcaatta gcgtactaaa
     1861 aaaactatgg gatacaaaaa gcaacagaca caatagaaaa ggaatcagat accaagctcc
     1921 aatctttccc actaataaat accaatcaac tgcaaaaata atgacaaaca gctgaaacaa
     1981 cgtaatgatg aaacacatcc gtttgatttt tcctttcctc caacatacct ttttttctgc
     2041 caagtaatgg acagatagat aataagaagg atacatcatc aaaaaataaa tcagatagac
     2101 aacacgagca gacagcacta atcctaaagt gaacaaacat cctagaacta aagcgaaaag
     2161 agcattactg ataagactgg ttaaaaaaat ccgacagaca tagtagttga gcttgtaaag
     2221 aaacatctct tctattggct ttttcatatt cagcccttca gaccgcttgt actgatcccc
     2281 tggacaagat atttattgaa gaataagaaa atcagaaaaa ccggtagcag cgacaacgta
     2341 gacatcgcaa acatactcgc gtaagtcgta ctagatccag cgtctgcaaa taatttgatg
     2401 gcaatcgata ccgtgtaaga tctcggcttg tttaaataaa tcaatggtcc catgaagtcg
     2461 tcccatttcc aataaaactg gatgatgatc gtcgtgatga tcgaaggttt cagcaaagga
     2521 aaaataattt tggtaaagac actatacttg ctgcatccgt caataatcgc tgattcatct
     2581 aactctttgg ggatgctgac catgaattgc atgatctggt aaatgaaaaa tgcttgtcca
     2641 aaaaaatgtg gtaaaatcag cggcacatac gtcccaacca aattcagccg attgtaaata
     2701 ataaactgcg gaatcaaaat cacttgtgca ggaatcatca tcgttactaa catgacgacg
     2761 aaccagaatc tttttccttt gaaatcgatt ctcgataatg cgtaagcaat acaagtcgag
     2821 ctcagaactg tccctatcgt cgaaactgca gagatgacaa acgaattcct aaagaaagta
     2881 aaaaatgaaa ttccagcaaa ccccgcccat ccttttgcgt aattttccca ttgaaaagtc
     2941 tctggaatca aattcagcga accactcata atctcttgat ccgttttaaa agaacctgcg
     3001 atcatccata agaccggata aatcatgact agacctaata gcaaaataaa accgtattga
     3061 aaccatttcg gtattattca tttcttcctc ctctcattca cacaacgacc catttttttg
     3121 atgatttgaa aatcagaaag gtgacagctc ccataatcaa cagcatgatc cacgaaatag
     3181 cactagcata ccccatagaa ttaaaagaaa aagcttggtt atacacataa agagcataaa
     3241 agttcgtggc atcattcgga ccaccttggg taatgataaa tgcctgcgta aaagtcataa
     3301 acgccgcgat tgtctgcata accaagttat acaaaatgat cggcgaaaga agaggtaacg
     3361 tgatcttgta aaacgtctgc catttgttcg ctccatcgat ctctgccgct tcataatacg
     3421 agcctgggat ctctttgata cctgcagcaa agatgatcat tgccgaaccg aattgccaag
     3481 cagacatcaa aacaagaggg atcatgacca gtttttgatc gctaaaccag gaaagcgcag
     3541 gtgcgcctaa tcgttccagg aaaatattca ctaacccgtt tttagaaaac aactcccgcc
     3601 aaactagagc gatagaaata ctgccaccga tcaaactagg cacataatac aaagaacgaa
     3661 agaaagtcgt tcctttgata ttcctcgtta aaagataagc aatcaataga ccaaagatca
     3721 gtttcacagg tacagatgca aatacataga gaaaagtgac tcggatggag ttcaaaaaac
     3781 gggcatcact cagcatcttt atataattat ccaagccgat ccatgagact ggatttccca
     3841 aacgataatc ggtgaacgaa taataaagtg acaagatcat cggtatcact gttatcaata
     3901 gaaatcctag gatgaatgga gaagcaaata cgtaaccgac cacattgtct tgatccaaga
     3961 accgttttag tttttctttt ttcatgcctt cctcctgctg ctatcctaga atgtttgacg
     4021 caaactcata aagactctct gctgcttctt ctgctgttac ttcttcataa atgacttttt
     4081 caactaaaag attgtattgg tcttgaattt ccggattcaa agcactatca aagacattca
     4141 cttcgccgtt gtccatatcg gaaatcattt ctaaataatc aaatgtcgct tgcaaagttg
     4201 catcatctgc acgagcgagc tgttctcgaa tattcgacat gattggtaca ccacgttctc
     4261 cttttaaaat ttcattggct tcttcactgt tttgaaagaa atcaataaac ttagcagctt
     4321 cttctttatg ggctgagctt tttgccatac tcagcatttg cgaagaacgt aaagggatac
     4381 cagattctcc atctgctttt ctgcgaggtg gattaatcaa ctgaatctcc atatcttcag
     4441 gagcccctga caatatttct gtcaactggt tacttgatag aaacaccatt gccgcttctc
     4501 tatccctaac cgcctgaaaa gacagatccg tcgtacttgc agctccacca cctggttctg
     4561 gatgggcccc tttgtctacc agatttttac gcagctggaa aaaatctgtt agatagtctg
     4621 gattttcaaa tcccaagctg ttagatagat cttcccgatt ataaaaattc aatcctttct
     4681 caacttgtgg tatttgaata atacagccag ctataaaatc ttcgaatctt gaaatagcat
     4741 agattcctaa ttcttcagtg atttgatagc tgatttgttc aaattcatcc catgtccaat
     4801 ttgaagctgg ttcagggata tttaattgtt caaatatatt tttattgtaa gcaatcgcaa
     4861 acgaattcac cccgttagac agtccaacta attgcccgtc gtaagtcgtc gttgctaaat
     4921 aggattcatc tgcattagaa atatcaatta tcccttgctc cacatattca tctaatggtt
     4981 caatctgatt gatgtattga ggaaattgat tgttcagttg gaaaatgtcc cagatatcat
     5041 ctgatgctgc tagtgtacct aattttgtgt aatatccttc ataatcataa aattcataat
     5101 cgatcgtcac atggggattt ttttcttcat aaagctcgat tacttttact gttgcatcgt
     5161 gtcggacttg cgaaccccac caagccatgt tcagcgtgat ttttttgtca tcttcttgcc
     5221 cacaagccgt caagaagaat aacataacac ttaaagctaa gatgattctc attcttttct
     5281 tcattctctg cacctctact tccattgtta tttttcaata tactgtattt ttatacagaa
     5341 catatttatt aaacgatctt tttcaagggg aataaaaacc aagacttgcc ttatcgttct
     5401 gcctaggatg aacaactcaa tactttcaag aaaaattaga tagtgctgat tcttctttca
     5461 tcgctaaaaa cacttcttgc ttcaatgatg ggaggataat tacgcaaaga aaaaaggata
     5521 tcagacaact gcccaatatc cttgacatag ctaaataagc atgcttcttc gtgtgcgctc
     5581 ttccctaaaa aacagaaaaa taactagtaa ttctgtatga aattaggtac aatatacacg
     5641 tcgatgcaca agcattgact atggcaactt gattggtcag ttcccgctga cttttcaagc
     5701 cccgacaatt tagctgctac taaattgtcg ggtgcctttt ttagttttta ttcgtttttc
     5761 attctataga aagcgcttca ataaatcaag acattttttt gttagaatgc taataataac
     5821 aaaaggtttt aactcttctt atgtacatta caggtaaaaa aacaaaaaaa tttctctttt
     5881 ttttgaattc tgaacaaata gtactggagt acaaaagacg aggaagagcc actatctgct
     5941 cttcctcgtc ttttgattct cacctgttat tcatgattct ttttctcagt ttcccgatat
     6001 ttttccattt tacgactaaa aagctctccg ctacttggtg gcgtatatgt gatggcattt
     6061 gctcctgctt gaatggtctc agcaatactt tcttccgttg gaccacctgt cgcaatgata
     6121 ggaagttcag gatatttttt acgaaaatgg cgtactgctt ctgcagtttc tttacctgca
     6181 ctgatattga taatcgaaat accagctgct aacttttcat ctatttctgt atatttcgac
     6241 gttaccgtac tgataactgg aatatcaact actcgacaaa cttcttttac tgtctctaaa
     6301 ggcgtaggac cgttcaaaac gacccctaac gaaccttgcg cttctgcgaa catgctcatg
     6361 taggaagaac gagcaccttg tgtcaaacca ccacctacac cggagaaaac tggaatgtca
     6421 gctgcctcaa taatgctttt agtaatcgct ggatgaggtg taaaaggata cacagcaatt
     6481 actgcattcg catttgtatt acgtatgatc gcaatatccg tagtaaagat gatcgaacgg
     6541 atcaattttc caaatatctt gatcccgctt gcttctaaaa tgatttccgg tactcttacg
     6601 atatcttttc tcaattccgt catgatttct ggcgtaaatt gttctgccat cagccacttc
     6661 tcctttgcta tctccggtca tactgattca attgatacgt ctcgatgacc tggatgattt
     6721 tttgcgcata accggggtct gtcgcataac ctgcatcttg caatgcttgt gcggcttcct
     6781 gatagttggt ggccgtaatc actttttcat acagcgcagg attccaatct actccttgga
     6841 cgaacagttg tgtatggtca tccatggact gttcccatga atcatacact ttgaagtctc
     6901 cttggatagt tatccattct tcgttaacaa attcctttgt ttccaggctt actttcttct
     6961 gatccccata agccttgatc ccgaaaagat tattgtattt gctagccaag gtactttttc
     7021 cccaatttga ttctaatata gcttgtccta aaatgatact aggaagcact ccataaccat
     7081 cttgtagttc ttgggcatgc ggaacaagcc gttcaataaa ttgctgatgg gattgcgctt
     7141 cattttcttg ctgattatca gtaaaaggag aagacagatt cttcaaggaa aaaacaaagg
     7201 ctaagagtat cactgctaca ccggcaacca ctgctggcca acggatattc ttgcgctttt
     7261 gctttttcca cttttttttc gccatgatca attcctactt ttcatttaat tcattcagat
     7321 attcttctag cgtcaattgg tgtttggtga tatactcagc gttttcttta ccgacataac
     7381 gtaagtgcca aggttcatat gtgatcttag ttatatcttc gtgtcctttt ggataacgaa
     7441 cgataaatcc atactgcgca gcattatctg cgatccattt agctccaggc tgatctccgt
     7501 aacttgcatc caatacagta gaaggataac tgttgtacca gttttgatcg acgacatcga
     7561 ctgccagacc tgtgtggtgt tcgctgtatc ctggctccgt aatcgtttct ttagtaatct
     7621 ttgtcgcctc ctcttctgat acaccttgac gactcatcac atcttggaca ttttgttcga
     7681 aaacttgttg ttgggatgat acagaccggt aagctgagac catcactaat ggaaatccag
     7741 ctgcttcagc agccttggca aatgcttcat attctggagc aatgcgtttg tcgatcaagt
     7801 atccgttact taatgatgcc aattgagtag actcatcaat ttctttttct aatttgtgat
     7861 ctggaccaac caaaactagc ttccaatcgt ctgttttaac atctggcaat tgtttggacg
     7921 tacgatcttt tgtttctttg tcctttacct tgctgctggt tgtttgactg ttcttttctt
     7981 tactagcagt tgttttggtg cttgttcgtt cgactgcttg attgccggct tgaacatgcg
     8041 aatcatcatc gctaaaaata acatagccca ttgctgcgat cataatcaca actgctccaa
     8101 atcctaaaaa tttattcact tgaaaaatcc tttcgttgtt cttcacgttc cccattcgcc
     8161 tcatgaacgt aagtttcttc cagattttca ttcacccaat cgagaagttc gattttttct
     8221 ccttcgatct gtttttgaac atctgctggc agacgaaaat tcaaaatatc atcatagccc
     8281 cattcatcat attgaatact tactttcaag tcaaggtatc taccagcctg ctgattattt
     8341 ttcaaaggga tcagctcgac ttttgctgct ttgctgctaa gccatttttg cgcgatgcgt
     8401 gttttgattt gcttgtactc tgcaacggca acgtttcgtt tttcgggaaa aataatcgtt
     8461 tgacgcaaaa tcttttgcgt tagcatccat tcataatcag tgaaaagatt ttttactttc
     8521 tgcatcgctt cagatggcag atttttctcg ccatctttcc atttttgcca ttgttctgga
     8581 ctgatcagca agtattcatc taaaaaattt ttttcagttt caaactgttc taataataca
     8641 ctcataataa gatcaatata tacttgatgc acgtttatcc cctcctttta actattctac
     8701 ttaagttttg atcaaaaagc acctcttaaa tcgctgcttt catttttttt aatgtttcaa
     8761 gtcctctcct ctttattctt cgtgattttt gttataataa tagacagaca agaaactcaa
     8821 tccaactatg aaggagcgtt ttcagatgaa aaaatatcaa tggggaatcg ttggtcttgg
     8881 taccatcgca catgagtttg ccgaaagctt caatcaagag acgagcgaac taacagcagt
     8941 tgcttccaga acttcagaaa aagcggaaaa cttcgctcac cgttacaata ttccaaaagc
     9001 ttacggaagc tatcaagaaa tgttagacga tgctgaaatc gacatcgtgt acattgctgt
     9061 accaaataga cagcatatcg accatatctt ggcagcattg gaagcaggca aacacgttct
     9121 atgtgaaaaa gccatcacga tgaataaaaa agaactggct gacgctatgc gcttagcaga
     9181 agaaaagaat ttgatcttag cagaagcgat gacaatcttc aacatgccac tttatcagca
     9241 gttacgctca ataatggata caggaaaact gggggcttta aaaatgatcc aagcaccttt
     9301 cggcagttac aaagaaccag accctaaaaa ccggttcttc aatccagaat tagctggagg
     9361 agctctgttg gatatcggta cgtatgctgt atctttcgct cgtttctttt tgagctctca
     9421 acctgaagta gttgcctcta caatggttcc ttttgaaaca ggcgtggacg aacaatccgt
     9481 cactatcttg cgtaacaaag aaaatgaact tgctgctgtc agcttgactt ttcaggcaaa
     9541 aatgccaaaa gtcggtatcg ttgcctttga gaacggttat gtgacgattg ctgattaccc
     9601 gcgtgctgat cgtgcggaga tcttatttac agatggtaca aaggaattca tcgaaagcgg
     9661 gaatacatct gaagcaatga attacgaaat aaagaatatg gtacacatga ttgaaggtga
     9721 cttgcccaat cgctctctct tcttgacaaa agatgtcatc gaaatcttgg atcaaatgca
     9781 agttctatgg aaaaatatgt aattttaaaa ggttgtgaaa aagctgttct gcatcaagta
     9841 ataagaacag ccaaacaaaa atagctcctc ctatttttcg tttggctgga cttattaccg
     9901 cagaggaaag cttttgaaca ccgtttaaga aagaggttgt gagcctaccg ttcagctccg
     9961 agtcacaagg agcaatttca aaaaacagct tctcatgttt gttgaatatt gtgacttgtg
    10021 accgaggagt tgacaggaga acaccgttta aagaagaggt tgtgaaatca gctttttgac
    10081 ctgaatatta gctgaattca caacctctat ttttggattt tttgttggga gaattctgcc
    10141 ttgatttcaa taaaactttt aaaagactgc aaaaactgga acaattctgc tcgatcctcg
    10201 aattcagggc gactagtcgg cagaggcatt tgccgataca atacataaac ttcttcaata
    10261 cgtgttaaaa tttgtctgcc atcatttgac tctgaaaatg tttctgcagt ataaatcaat
    10321 aaaccataga tatgttttcc ataataagct ggttgctgga ttctctctag attttccagc
    10381 atgtctttaa tcacattggc ctgtgctctt ctcatagaga agtaagtttc gtagtatagc
    10441 ggctgtctga gccagtagtt ttcttgatgt tctctggcat cttcttggct ttcgcgaatg
    10501 aatgtcagta aatgttcgca tttgatctgc acatcttctt tattttctga taaaagaaat
    10561 tctgccattt gtctcaaaat atttcgaaat tctttttcga tctgtatttg gttttccttc
    10621 agctgcttcg tacgatctgg catgtaaaca tttgcaagca aagcaaatac taatccaatc
    10681 gccattaatc ccgcttcatt gccgatcagc tgccaagaca tgttttcctc cactaaataa
    10741 tgagtgacca aaaccgaatt tactactatc ccttcggtta atttgaagcg agaggcaagc
    10801 gggatgaaca ggaataaaaa aagactgaag ccaaaaatag tatacccaac agcaaaaaaa
    10861 ctgaacagtg ctgttgccgt agctaatata aaggctgcga ttcgattcat gcccgtacgc
    10921 aatgtagagc gcttggtatt tcctacactt aataatgcaa tgatccctgc agctggccaa
    10981 tacaacagcg ataagctacc cgctgtgacc atcgaaaaga agacactgat cactgttttg
    11041 actgttcgca ttcctatacg catttacttc cccctttttt attttaatta tacttgaata
    11101 atgaaaaact agctatcaga caaaagttta ggtatgatag taaaaagacc aaaaaggaga
    11161 atctctatgc aatttggccg ttttcgacta ggtatgcgta cgatcaaaag tgccttagct
    11221 gtttttttat gtatcttgtt ttttcatttg actcaacgag gaatcccgat gattgctgca
    11281 ttatctgctg ttttttcttt gcgtcaggac ttgactacaa ccgtttcttt tggaaagtct
    11341 aggatcatcg gcaatacatt aggtggtaca ttagctatca tttatttttt tgtcaaaagc
    11401 tattttcaca atgatttttt ggtagaattg atcttgcttc ctattctagt tattattgtg
    11461 atcgtcgtgt cagatggtat taacaataat tccggtatca tttccgcaat agctacgttg
    11521 ttgctgattg cgttgagcat ccctcaaggc gaatccgctt tgtatgctat ccaacgtgta
    11581 ctagatactt ttatagggac attcattgca atcggtatca atttctttct tcgtcctcct
    11641 gaaacagaaa agaaggaaga actatcagaa gatttggtcg aactccaaaa gaaagaacga
    11701 ttgctaagaa caaatttaca agagatagaa gctaagatcc aaaaacaaaa gaaaaatagc
    11761 gaggactaat tttcttttgc aataaaataa aaaaatggag agaagaactt taaaaagtct
    11821 tcctccattt ttgattgaac ggtgaagtgt actactttat tcttccttca tcgagttttg
    11881 ttctgcctct tttggcgctt catacatatt tacttggcta gtagtcccac gtgtcgacaa
    11941 attctctacg atttccttca agtcaagtcc tgttgtcgct tttagtgttt cttgagtaga
    12001 agaaagcaaa tttgtggcat aatttgtcac tcggttcgct ccactggttt ctcctgaact
    12061 gcttcctgta tcgacaacag agattttttc gatatttcct agcggctgtg ctgcttcgcg
    12121 catcaattgc ggtagcatat cgataaccat actcaagaca gctgcttcgc catattcctt
    12181 gaaggcattg gcgatctttt gttttgcttc ggcttctgct tttccttttg ctagtgctgc
    12241 ttcggcttct gcttgtccag ctaaacgtgt cttattggct tccgcttctg ccagtgcttc
    12301 cacacggaat cgttctgctt cggcttcagt cacttctttg actttttgag cttgtgcttc
    12361 ttgttcttta gcgtaccgat cggcatctgc ttttttcttc acttccgaat catattgctt
    12421 ttcacgacgt gtgatttctt tttcttccag ttcgatttgt ttttgacgtt ctacgatttt
    12481 aatctgcatt tcctgttcga ttacttgttg ttgtgctcgg gcactttcta aattgtaggc
    12541 ttggtctgct tttgctttag caacatcttg ttcttgtttg taggttgcta atttcagttc
    12601 tttttctttt aatgattctg cgatctctgt ttgtcgctgt aattcagctg cttgtgattc
    12661 tttttctgct tgagcttttt tgatacgcgt ttcttttaga gcttccgctt ctgctatatc
    12721 tgcatctctt ttgacttggg cgattcttgg tttccctaaa gagtcaagat aaccgttttt
    12781 gtctctgacc tctttgatag tgaaagaaac gatgatcaac cccatttttg caaggtctac
    12841 agaagcgact tcttgtacgc tttgactgaa cttatcgcga ttttgataga tttcttctac
    12901 agtcatagaa ccaagaatcg agcgcaagtg tccttctaat acttctcttg cttcattttc
    12961 cagttcttct cttgtcttgc ctaaaaactg ttctgctgct gtagcaatct cttctacaga
    13021 tgaaccgatt ttgatgatcg aagtaccatc acacatcacc ggtacgcctt gttccgtata
    13081 cacttcaggg gtggagacgt ccaatttact cgataataga ctgatccgat tcgaacgttg
    13141 aaaaactggc aagacgaatg cgccacctcc acgcacaatt ttgattttgt tattgctttc
    13201 atcaatgtgc acatttttgc ttcctaaata acttccactg atgatcaacg cttcatccgg
    13261 ttttgctgtc tggtacttcg ctacaaagac gatcagcaac atcagtaaaa tcaaagctgc
    13321 gattgcgatc gcgataaaaa caactggtat ttccatttaa cacacgctcc tttaagcttt
    13381 tatcatgctt tcataaggca tgacataaca gattctttcc ttgatctcaa tgatcaatac
    13441 tttcgttccc cgctcagctg tctgttcttg ggggctgtaa aaacaagctg gacgactaat
    13501 ggaaccggta acacttttca actgaatttc tcccatacct cgtacaggga tcggtgtgat
    13561 cacaacagct acttgtcctt ccagagtttt ttcagaagac gacatcgttg attcggcatg
    13621 tttcatcggc atcaatatat agaaattaag caaaaatacc aacactatcg atatgcctcc
    13681 actagcaata aataaaaata aagatgattg atctgttaaa cgctcaccta gaaaaccaaa
    13741 taagcttgtg aaagttaacc aagggataat aagcatcgga tcaagcgggc catcaaaatc
    13801 gaatacatct ccaaaaataa tcaataggac tgccaatcct gcacaaatca gcaatgtata
    13861 ccaataaatc gtctcaagtg cgattccccc tatcatatac acgcctcctt ccatttctac
    13921 gtctatcata ccaatgtttg aaaattctgt atagcttctt ttaccgactc agtggtgttt
    13981 tttataggaa ggttgtgttt atcgttgaca atttgttaaa tttttgtttt catgttaaaa
    14041 ttttcacaat aaattctttc gaggtagcct atgtttcctt tttttaagaa aaaaccatta
    14101 aagctccaga acacgatcat ccttgtcgtt tgtttctcca cattcgtttc tttgattgtt
    14161 tctgccatat tgatcaggaa ttttattctt tcgatgaacc gatctagtgt gtcagataaa
    14221 atctctagca ttgctaaaat cgttgccaat gataaactca tccaaaaaaa gctgcagact
    14281 ggtgacccga cacatgcgat tcaaggaata acaaatgaaa tcatgattgc atcgaattta
    14341 gattttatcg ttgtcatgga ccctgaaggc attcgatatt ctcacgcaga cccaagttac
    14401 atcggtcttc ctttctcaag tcatgaagat gtgaaaaaag tgattggagg acaagaattt
    14461 ttctctgaaa aagaaggaat gttagggttc ggcttccgct atttcgtccc tatttattat
    14521 gatcaagaga ttattggtgc agtttgtgtt ggcttaacaa acaacacgat tgacaatcaa
    14581 gtcaaagatg gacaaaaaat gattgttttt tcattgatta ttggacttag cgtaggcatc
    14641 ataggcgccg tttttcttgc ccaaaaaatg aaaaaagtac tgcttggact tgaaccacaa
    14701 gaaatagcag aacagttaag tgaaaaaacg attatcgaaa atgaagtaga tgaaggaatc
    14761 ttagccatct cttctgataa aactatcctc ttgatcaata cacctgcaaa aaacattttc
    14821 taccaaacag agcagctatt ggatttcgaa gtaggaaaaa aatcagtgat aatctatatg
    14881 acgttttgtt taaagagacg attgagcatc aaacgaccgt tcggactcaa tccatgtatt
    14941 taaatgggat agaaactatt tctagcgtta cacctctttt tagcaatgac cgttttttag
    15001 gagcagtcgc aactttacga aatcgatcag aagtaatgaa attaatggag gagcttgggg
    15061 ggacgaaaca atatataaac tccatgagag ctcaaaccca tgaattcatg aataaacttc
    15121 atgtcatttc tggtttgatt gatttgaaga agtatgaaga agtcacccat tttatccagc
    15181 agctaaatca aaattataaa aaagagattg gccaagtctc aagtctgatc aaagtacctg
    15241 caattgccgg ttttttatta ggcaaaataa acgaagccaa agaacaatct gttgaaatgc
    15301 tgttagaaag cgattctttt atccctaaaa tggagatgga tgactccatc cattcacttt
    15361 tgcaagtgtt agggaactta cttgataatg caaaagaagc cgtgctacaa aaacatgaaa
    15421 gaaacggaca ggtccggatt catttaaact atgacacaga agctgaagta ttcatcgtga
    15481 ccgtctgcga tgacggtgtc gggatatcag aagaaatctc agaaaaaata tttacgctag
    15541 gtttttcgac gaagggaatc aatcgtgggt ttggattgaa tctcatccat tcaatcgtca
    15601 aacgccacgg tggattttta gaattttcca aacaaacaaa tggcggtaca atcgtctatc
    15661 tagaattacc aaaaaaggaa gatcaaaatg atgacaaatg tcctgataat cgaagatgat
    15721 ccaatggttg cagaattgaa tcgtactttt gtggaaagca tccctaatat gaacgtaatc
    15781 gaaagtttca gaaatgtaga agaaggaaaa aaatatttca gacaaaaaaa cgcagatctg
    15841 ctgctgcttg atgtatacat gcctggcgaa agtggtatcc attttttatc ttggcttaga
    15901 cagcaaggaa atgatatccc agtgattttg atcactgctg cagatgatat cgaaaccata
    15961 aagaatgtga tccgctacgg tgccttagat tatttgatca aaccgttcac tttgcaacgt
    16021 tttcagctgg cgatcgaaaa gtatcttgct ttctctcaga caatccaaac gactgaaaaa
    16081 gccgatcaga aattgatcga tcaagtattc aaccacacca ctcccattca cgaaatacca
    16141 tcattcatcg atttgcctaa aggtctgtca aaactgactt taaaaaaaat aagtgcattt
    16201 attctaaaag aagatactat tttttcaaca aaagaactgg ctcaaaaaag ctcactgtca
    16261 cgtatatccg tcaagaaata tttgaatttt ttagtagaac aagatatatt gaacgaaaga
    16321 atcaaatatt tggaaatcgg ccggccgcta acctgttatg aattgacaaa gaatgggcgg
    16381 aaaaagctca cagattactg ttgagtcatc gaattatccc atacaaaata gtccaagtat
    16441 acagaagtaa caactgttta cttggactat ttttaaacat tttattgacg aatatccatg
    16501 ttttcttatc ttaattgacg ggctttagga agagatacct catttttcgt ttttccagtt
    16561 tctattattt cacgaaagtt ctcgaaactt gtcgaaatca tatttgataa tgcctcatct
    16621 gtatttgaac ctacatgcgg ggttacaaga actctagggt acatttctac taattgctgg
    16681 atagcagggt ctggaatcgt ctcccatggc ttgaaggatc ggaaaaaaac ctctgtttca
    16741 tttgcaaaaa catcggttgc aaaaccagct aaatgattgc ttttcaaagc tcttaaaata
    16801 gcttcattat cttgaagctc tccacgggct gtattaatca agatgctacc ttttttcatt
    16861 tgagctaaaa atccttcatt gatcatttga tcgttttcac ctgggatatg aggtacatga
    16921 atactgacaa tatcgctttt ggctaataac tcttcaagcg agtggaacgt caatactttt
    16981 ttagcagctt ctgtctgatg aatatcgtag ccgatcactt tggctcctag tcccttaaac
    17041 aaaacagctt ccgtcaaccc tatcctgcct gttcctatga tccctactgt gcaatttctg
    17101 atttcacgac taaacatttg ctcatccact cgaaaatctt tgaaagatgt tcggatcgtc
    17161 gtatacgcag tatttctcaa aagcatcatg gcaaaagtca aagataactc tgctatcgca
    17221 tttggcgaat acgatggtac acgggctact gtcataccaa aatcagctgc cgcttgtaga
    17281 tcgatatgat tgactcccac agtccgtgta aaaacataac gaatcccata ttcctgcatt
    17341 tttgcaagat tggtctcatc tgctatacaa tttcccctca acaatacagc atcatggtcc
    17401 tttgctgaca cgatattgtc atgtgtcaac agttcttcat aaagactgag atcataatgg
    17461 taaatattca atttttcaaa aaaatctatc tcgttcggac gaacaccgta acatgcgatt
    17521 ctcatacttt cttcctccat ttcttatgaa aataaaccta tgccatgaat catttgtaat
    17581 acaacgatcc atacaggtgt cagtaccaca gcaaaaatag ttgacagtaa ggatgcattg
    17641 gaggctaaaa gtgcctccct atcaaaactg atagcatagg ctgctgccac tgttgcagct
    17701 ggtgtagcca tcatgataac gattgttgcc agtgcttcaa aattgactgg tagaatattc
    17761 gttgccgtca aaactgctag aatcacgata ttcaatgcag gtacaaatac cactttgact
    17821 agactgtaat accatgaagt tttttcagat gcagcttttt tgaagcttac ttcacctaat
    17881 gttgcgccga ttgctagcca cgccaatggt gaacttaaac cagctaaata atccatcggc
    17941 tgaaatagcc acggtgcagt ctggtcaatt cgcaaaaacg ctacttggct tatctctcct
    18001 tctgcacctg taactgacac ttgtggcaga tacccttgga acagccaaat gaaaaggccg
    18061 gcaaaagtag cgataatgat tggattgacg aacatcgctt tgatattttt agcttccatc
    18121 ttcaagccac tcatttttat gtatccataa gaatacaaaa agattcggta gccaatattg
    18181 aaaattgagg aatacatcac gccgacagga ccataaatcg cactgacgat aggcgttccg
    18241 aaaaaggtcg tagaaccaaa gatcgtcagg acacgcaacg ttgtttgctt atccccttta
    18301 tagcttaaga aaaaaggttt tgaaactaga ataagaataa tataaataac taatccccaa
    18361 atcaagacat tgattccttg catcagtaat tctgtattga tatcttgcat gaaagctcga
    18421 aatgccagtg ctggcaaagc cactgttaag acgactttcg atagaatttt tccaacagat
    18481 tcagaaaaaa tccccttttt tctacaaaca aaccctaata atataataaa aactgttgaa
    18541 gtgattgcac tgacaatcga catatctgtc aaagtcgatc gaaaaatgtc aaataagttc
    18601 atctttctcc tcctaattat gttcatttat tc
//
LOCUS       DAFFSV010000047        18466 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFFSV010000047 DAFFSV010000000
VERSION     DAFFSV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.047"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(97..477)
                     /gene="yajC"
                     /locus_tag="LR110_001487"
     CDS             complement(97..477)
                     /gene="yajC"
                     /locus_tag="LR110_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBL4822453.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(574..1719)
                     /gene="tgt"
                     /locus_tag="LR110_001488"
     CDS             complement(574..1719)
                     /gene="tgt"
                     /locus_tag="LR110_001488"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBL4822454.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1937..2635
                     /locus_tag="LR110_001489"
     CDS             1937..2635
                     /locus_tag="LR110_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL4822455.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2803..5442)
                     /locus_tag="LR110_001490"
     CDS             complement(2803..5442)
                     /locus_tag="LR110_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL4822456.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6061..7092)
                     /gene="queA"
                     /locus_tag="LR110_001491"
     CDS             complement(6061..7092)
                     /gene="queA"
                     /locus_tag="LR110_001491"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBL4822457.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7285..8460
                     /locus_tag="LR110_001492"
     CDS             7285..8460
                     /locus_tag="LR110_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL4822458.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8464..9105
                     /locus_tag="LR110_001493"
     CDS             8464..9105
                     /locus_tag="LR110_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822459.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9102..10019
                     /locus_tag="LR110_001494"
     CDS             9102..10019
                     /locus_tag="LR110_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4822460.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10016..10687
                     /locus_tag="LR110_001495"
     CDS             10016..10687
                     /locus_tag="LR110_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822461.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11228..11716)
                     /locus_tag="LR110_001496"
     CDS             complement(11228..11716)
                     /locus_tag="LR110_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBL4822462.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11917..12183)
                     /locus_tag="LR110_001497"
     CDS             complement(11917..12183)
                     /locus_tag="LR110_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL4822463.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12183..12446)
                     /locus_tag="LR110_001498"
     CDS             complement(12183..12446)
                     /locus_tag="LR110_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL4822464.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12922..13107
                     /locus_tag="LR110_001499"
     CDS             12922..13107
                     /locus_tag="LR110_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL4822465.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13249..15072)
                     /locus_tag="LR110_001500"
     CDS             complement(13249..15072)
                     /locus_tag="LR110_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBL4822466.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15670..18375)
                     /locus_tag="LR110_001501"
     CDS             complement(15670..18375)
                     /locus_tag="LR110_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBL4822467.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKINDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         5261 a         3917 c         3462 g         5826 t
ORIGIN      
        1 gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg taggacatct
       61 ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct tctcttctgt
      121 ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg gttcttctgt
      181 tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga ccgttttgat
      241 tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg tccgtttgtc
      301 attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg aatcacctgg
      361 tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg aacgtgacat
      421 aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg ttcccatttt
      481 tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg ctgataacac
      541 tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt tttctttatt
      601 gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat ccatgattgc
      661 ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag aagtcaaacg
      721 aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg tatagttttt
      781 acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat atttcgcatt
      841 tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga ttcgagtagg
      901 caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg aatcagcagc
      961 accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag tgaattctaa
     1021 tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg agtaaccagg
     1081 gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa accctgctcc
     1141 ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca atcctcgttc
     1201 tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt catcgaatgg
     1261 tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga tgttgattgc
     1321 tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat gcacgccttc
     1381 ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg aatctgttaa
     1441 aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta ctaagtcttc
     1501 accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac ccatttcttt
     1561 caactcttct ggggacattg tttttactgt agctaatgtt cctactggca tgaacattgg
     1621 tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag tatgtttttc
     1681 tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta ctttctatat
     1741 aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc aagttccctc
     1801 ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga ataacaaaaa
     1861 aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg ttattaaaga
     1921 aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa aagagagtct
     1981 aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga ttggtattgc
     2041 tactattctt cttattatga ttcctattat cttgtttgcc accaccatat atgatggatc
     2101 agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg gtggcggaat
     2161 tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt atcttgatat
     2221 tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg gattcaaagg
     2281 tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta caacattatg
     2341 gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc aaagctattt
     2401 tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg atacgatcac
     2461 tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag atctttcatt
     2521 tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat ggctcagccc
     2581 atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata tttaaaagag
     2641 agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag gcagtaaaag
     2701 aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca cctccttttt
     2761 gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct cttcttctgc
     2821 ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga tagataaaat
     2881 ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt cttgcgtaaa
     2941 tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt ctaccaaagc
     3001 tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat tgaacacatg
     3061 cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat gctgacttgc
     3121 gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa acaaggttac
     3181 gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag caaagatccc
     3241 ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct ctcttcctaa
     3301 agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca cagctgtaaa
     3361 tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg aaatattaca
     3421 gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc cttgactgac
     3481 agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg cactttgcgc
     3541 cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca acgctggtgc
     3601 atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc aagatttgac
     3661 gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt tgacacgcgc
     3721 atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt ctgacggatc
     3781 attcaagata cctaattctt tcgctatggc actggcagtc accacatgat ctccagtgat
     3841 catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac tttctggacg
     3901 tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca gtgcttcaga
     3961 agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca ctctcagtgc
     4021 ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg aactaaagcg
     4081 cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc ccatttggtg
     4141 aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc gtggatattt
     4201 ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga tggctacttc
     4261 tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca catctgtaca
     4321 tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt cgatattcgt
     4381 tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg tcagcgttcc
     4441 tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg gcaactgtcg
     4501 gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca acgtaacaat
     4561 aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca tgaacatctc
     4621 aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag ccgctaaagc
     4681 gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg ttttttgcat
     4741 agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc caatggcagt
     4801 gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca tattaagttg
     4861 gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa ctgcttcact
     4921 ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca aacgggcatc
     4981 ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca gttgctctga
     5041 ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat tcatatcttg
     5101 caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata ccgagttgat
     5161 cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg tagcaaatgc
     5221 ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat cggacaagct
     5281 tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat tcaatccctg
     5341 cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat ctgcttctgt
     5401 taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc tcctttaaaa
     5461 tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt tacttcaaat
     5521 aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta tatttgcaaa
     5581 cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca aatagacaga
     5641 agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag gtttcaccca
     5701 gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt tggatcacga
     5761 agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa cgtctgaaaa
     5821 ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa aatttttgtt
     5881 taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac aatcatttct
     5941 gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct cggctttttc
     6001 tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct tttcataacc
     6061 ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt caatcgcatg
     6121 ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca tgactaaagt
     6181 cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt catagccagg
     6241 cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa atttcgttcc
     6301 aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc cgccattttt
     6361 acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt cactatgcat
     6421 atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc ctacatgtag
     6481 tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt ctttcgtaaa
     6541 atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga cagtttgata
     6601 gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt ctcccaatga
     6661 ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc cgccgtgttc
     6721 caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc ttgtcccaac
     6781 tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg tattcgttag
     6841 aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac gagcaggcag
     6901 aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa tgatttcatg
     6961 gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac gagaatgatc
     7021 gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat caaaatcttc
     7081 tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta tcatgttttt
     7141 cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct aaataaaaaa
     7201 actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata gttcttcatt
     7261 gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa aatttataaa
     7321 ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga gttcatttgt
     7381 tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa ccgaatgact
     7441 gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat tgatccagtg
     7501 gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc tcacatgacg
     7561 atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga agaacggaat
     7621 aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt agatcgctac
     7681 ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc ccttgcagcg
     7741 aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat cactcgtgat
     7801 tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat cgtatttgta
     7861 acccatgaca tggatgaagc gattaaacta gcaagccgta tcgccatcat gagtgaaggc
     7921 cgtgtgatcc aatttgatac accagaaaat attttacgtc atcctgcaaa cgatttcgta
     7981 gaagagttga ttggagaaga tcgcctacta caagccaagc caaatgcgac aagagtcggc
     8041 gaagttatgc taaatacagc aatcacgatt acaccggaaa aatcattaca agaagcaatc
     8101 aaactgatga gggaaaaacg tgtagatacg ttgttagtta ccgataattc gaatgtatta
     8161 aaaggattta tcgatgtaga aacgttgaat aaaaaacgag gaaaagtatc cagtgtcggc
     8221 gacattttga ataaacatgt tttttacgta aaagaatccg cctatcttcg tgataccttg
     8281 caacgcattt taaaacgcgg gctgaaatac gtgccggtcg ttgatgaaca gaacaaagtc
     8341 gtaggtatcc tgactcgtgc atcattagtg gatatcgtct acgatgtgat ttggggagaa
     8401 gatcccgcaa ctactgatgt tgcagagtca gctactcctg aagaatctaa ggaggtctga
     8461 cctatgcagc agttttttca tcagcacggt tcagaaattc tctcaaaagg ttttgaacat
     8521 ctttatattt caggacttgc tttgatcatt ggtatcatca ttgctgtacc gcttggtgtc
     8581 ttactgacac gttttccaaa agctgccacg attgtgattg gcttaaccag tgccttgcag
     8641 acaatccctt ctcttgcttt attggcattg atgatcccgc tatttggcgt aggtaaatta
     8701 cctgcgatta tcgcactctt tatttattca ttattgccga ttttacgtaa tacgtacatt
     8761 gggatgaaaa atgtcgattg gaattatcgg gatgtggcaa aaggcatggg gatgactgag
     8821 ttccaatcca ttttttccgt tgaattgccc attgctatgc caacgatcat ggctggtatt
     8881 cgtttagctg ccgtttacgt gattgcatgg gctacacttg cctcttatat cggcgctggt
     8941 ggtttaggcg atttgatttt tagtggactg aataattacc aaccagatct cattttggca
     9001 ggtacgatcc ctgtaacaat tttagcacta ttggctgatt ggcttctagg tctcttggaa
     9061 caccggctga caccgatcgc acttagagag gagaatgaag aatgaaacgt atactaaaaa
     9121 tgaccatggt tcttttctct cttcttatac ttgcgagttg ttccttccct ggtcttgcca
     9181 gcaatacaga tgaagacacc atttcgatca caggtggaat cacttcagaa gcacagatct
     9241 tagcaagtct agttgccgga atggttgaac attatacaga taaaaatacc gcaatcatca
     9301 acaatctggc tactacaact atcaaccatc aagcgatgat gaacggagat gcttcaattt
     9361 cagcggctcg ctacacagga acagatttaa cgactacatt gaaccttcct cctgaaaaag
     9421 atccgaaaaa agcgtttgct accgtgaaag atgaatttga gaaaagatac ggtcaaacgt
     9481 ggttcccgtc ctatagtttt gaaaatacgt atgtctttct tgttcgaaaa gacaccgcac
     9541 aaaaatacca tctatctaaa gtcagcgatc tgaaaaatgt agcggatgaa ttagtagctg
     9601 gtgtagatac ttcttggatc aatcgtaaag gagacggtta tgacggcttc caagaaactt
     9661 atggcttctc cttcaattcg atattaccta tgcagattgg acttgtttat gacgcagtcg
     9721 aagcaggaaa aatggatatt gttcttggct actctacgga tggacggatc gccagttatg
     9781 atctagtcat gttggaagat gatcgtcaat tcttcccacc atatgatgct gctccagttg
     9841 tagacaacaa gatattagct gatacgcctc atttaaaaga agccttaaat aagttaggtc
     9901 ataccatcag tacggaaaaa atgcaggaat tgaactacga agcagataat aatctagtgg
     9961 aaccatcagt agtagcacaa cgtttcttac aagaaaacca ctattttgaa gggaagtgaa
    10021 gcaattggat aaaatgaatg tctttcaaca atttttctat tatttccagg aaaatggctc
    10081 ctatatcttt gcacaattta tccgtcactt cctgatttct atctatggtg tcctctttgc
    10141 agcagttgtc gggattccgg ttgggatcat gatttctagg agacgtaaat tagcaaactg
    10201 ggtgattcgt ttggcgaata tcatccagac gatcccttct ctagccatga tctccatttt
    10261 aatcattgga ttaggcctgg gagtcaatgt cgtgattgtt actgtatttt tgtattccct
    10321 cttaccgatc atcaagaata cgtataccgg gatgatccaa gtagacaaaa acatccttga
    10381 tgtcggcaaa ggcatgggga tgactgctag acaacgacta ttcatggtag agttaccact
    10441 ctctgtttct gttatcatgg caggcataag aaacgcgtta gttgttgcca tcgggataac
    10501 cgcgatcggt gcattcgtcg gtgctggtgg tcttggagat atcattatcc gaggaaccaa
    10561 tgcaacagag ggcacatcga ttattttagc aggtgcattg ccaactgcat taatggcgat
    10621 catcacggac tggcttttag gtattttgga aagacggcta gatccagcca gccgtacatc
    10681 acgctgatgt tattgtaaaa aacaaaagct tctgcgacca tttgttggtc gcaaaggctt
    10741 ttttagttct gtcattttta aggcaataat ccactctgat tcttccctcc ttatgggcaa
    10801 agagttgctt ttcttagcta tttatccatt ccgtatactc caacttcata ctgccaacgg
    10861 gttcatatct gcatccaccg actaagtttt tttcttatcc gctaaggcat tttgccaatc
    10921 cattttccca tcactcgtca tggaattcat accacatgat ctctaaatac tcgtctgctt
    10981 gttctttgaa ttgggcatca attcttacat cctttggcgt ggctttcatt taagcctttt
    11041 ccatcagtcc tcgattgttc gatgccttca gctgcatcat caaaactggt gactgccgta
    11101 tcggtaactt cgatcatctt ttatacaatc gggaacttgc catcctcatc cttggctaaa
    11161 aacatatcta gccattccag cttctcgcca tgggaggttt gcggttccta aaaccggtac
    11221 acgattctta tggactgcat ccgtcgcaac tagacttggc gatacgatga tccttcacca
    11281 gaagagcacc cccgtaaacc aattgatcga tatattgcca ataagaaaag acattggcgt
    11341 aaaagatatc gatccctcgt gatgcattgc cgttgatact gctattcatg gccgaaagcg
    11401 ccacgacttt aacttcttcc gtttgagtga cattcatgtt cggcaatgtc gctttggata
    11461 cccgctctgc taaaggaacg acactggtat tgtatttcgt atctgtatct tttccaaaag
    11521 accatgccaa cgaatcctct gggaaccaat aagacgactc cggttgattg tccattgctt
    11581 ttttcaacat acttttggct gtttctcgat aaacgacatt tccaattgtc ttcgcctcac
    11641 tttggcgata atcgatggtc gttgccactg ccaacactaa caacgatgct ccaattttac
    11701 tgattttcct tgtcacactt cttcctcctt gttttacata aacccgtcaa cattccattc
    11761 ctaaactcaa acaatgaccc ttcaaaggtg gagatatagg aacaaattaa agaaatcatg
    11821 caataaatcc aagaaggttg gttttagaac aaaaaaagag tctccttttt ggggagactc
    11881 tttgttaact atctaaaaag gttggctctt ctcacttcac tcataatgtg tccaggcaga
    11941 ataaatttct acaactttat tttcctcatc aactttgtaa accactcgat gctgtctatt
    12001 cagcctgcgt gagtatctgc cttcatgtgt aggtctcagc ttttcaaaag actgcgtggg
    12061 catatatgga tcttccttca atgtctgaat cacttcttca aattgtttct ttaaattcgt
    12121 ttgcttgatt tttcttaaat ccactttcgc agagtttttg attgcgactg tatagttact
    12181 catcaaagag cttcccaatc aatatcatcg atattcgtaa agctactgtc gtctttttcg
    12241 cggtctcgta ctttatctaa agtacctgtt tgctcaagaa aaagggtttc ttgaatcgct
    12301 ctccagtctt ccagcccaat caatacagca ttgtttccac tacgatcact aataatctcg
    12361 atttcagtat gattttcatt tacctctttc aataactgat aaaaattttt acgagcagtg
    12421 ctaggattca tcgttgtatt agccattgtc tctcctcctt tttctgatat aattatagcg
    12481 tacttgatta agtacgtcaa aaaaatacaa taaagcactt aaattcagca aacttttcct
    12541 cttttttaca tagagaaaaa gacccctttt tagggaggat ctttgtccac gctcacttct
    12601 tactccttta cgactagcgg tagccaaacg taaagtaatt ggtaacaacg tatcatcttt
    12661 agcaaaggtc gaatccaaag cctgtgagtc tatgcaacaa caccaataca cgaaggttcc
    12721 tcgttgcagt gtgacttgat tgatatcttg aataacctga ggatacgcac gaaagatttg
    12781 taacggttat tcaaaactga ttttggccgt caagtcttct actgcctcaa aaatgattgc
    12841 ctctttcgtc tcctgtcatt catgttcact atcgatttga tacttgctta gaccgttggg
    12901 tttgtgaatc accaattaaa aatggcactg agttatggta acaaaaaatg gtcacttgac
    12961 gaggctgaga ttcctactta tgaagacaca tggcacttaa gagtcgaggt aaaggaagaa
    13021 gctgaaaata taggaggctg tacattattt ggcaaaggat tcggaaatta tgatcaagac
    13081 atcaaaatca aaatgtttta cagctaaata ggaagaataa taacatttca tacgctgttc
    13141 ccatctttaa tggcttatag agggcacctt tagctgagaa taagacaaaa cagaccctct
    13201 agcttcaatt aagagctaga gggtctgttt ttgattttaa aacttggttt atttcttcaa
    13261 atggtagcta taggtgctga caacgatatt ttcacgccaa gacaatcgca atcttaaccg
    13321 caaacttgtt tggttatgga gaatattctg ccagcgtctg tttggtacaa attgaggaat
    13381 caacacagta gttgtatagt tttgtttcgc tgcattttta cttacgatat ctacataacg
    13441 gataattgga ttttcaatcg agcgataaga agagtggacg attgaaaaac ggacatctgg
    13501 gaaatgcttt ttaaattctg cttcgatttc tttttctttt tcgacatctt cgtctagcga
    13561 aacatgcatc gcaacgacat aatcaccgat cgaacgtgcg taattcaacg caccgatatt
    13621 tacccgtgtc acattcccga ctaatacaag aacggtattc ccgtcataat catgtagttt
    13681 tgcatcattt tctaaacgaa gctgttctgc tactttttta taatgatcat ggatcttata
    13741 aaaaatgaac ataacgattg gcataatgat gaagaaaggc caaatgtccc ctaaccgata
    13801 gacaaacaaa atcgcaatga ttgcatatga gatgaacgca cccgtaatat tggcaatcga
    13861 tttgcttaac catctttttc cttctttttt ccatttgacg accatccctg tttgagataa
    13921 agcaaatgga atgaacacgc cgatagagta taacggaatc aagcgttctg ttgagccatg
    13981 gaaaataaac agcaatacga tcgaacctag cgctagtgtg ataatcccat tggaatagcc
    14041 tagtcgatcc ccacgatctt gatacatgtg aggcatgaat ttatcttttg ccagattgta
    14101 tgcaagaact ggaaaagcag aaaaaccggt gttagcagca actgctaaaa tcagcgctgt
    14161 agcaaattga aggacgtaat acaaaatacc atgtccaaag acagctttac caatttgaga
    14221 caaaacagtg acttcttttt caggaacgat cccataccaa tagttgataa atgtgatccc
    14281 cacgaagaag aaacctaaga tcaatgccat catcgtcaat gtagcagcgg cattttttgc
    14341 tcttggcttc ttaaagaatg gtacagcatt actaatcgcc tccaccccag ttaaagagga
    14401 agaaccagaa gaaaatgctc gtaaaatcaa agcgatagaa acacccggca cagccgctcc
    14461 tggaagtgca gtagcttgca atggcacagc acctgtcacg atattatata aaccaacaac
    14521 gatcagtaac gagatcacaa ggataaatgt gtacaccgga aataatagga acgatgcaga
    14581 ttccctcagc ccacgaagat tcagcatcat cagtagcaaa acgattgtca cagaaatagc
    14641 gacttgatga ccgtagagtg ctggcaccgc tgatgtgatc gcttcagcac ctgctgagac
    14701 agatactgct actgtcaaca tgtagtcgat caataaagag ccaccggaaa tcaagccagc
    14761 attctttcca agattctcac tgctgacgac atatgctccc ccgccatgag gataagcatg
    14821 gatgatttga cgatatgata gcgttaatga aatcaataaa ataataacaa aagcggcaat
    14881 gggtagcgaa taccagatgg ctgcagcaga caaagcgact aaaacgacaa cgatttgttc
    14941 cgtaccatat gcaatagatg ataacgcatc agacgacaat aatgccaaag cagcaaatcg
    15001 ggttaatttg tgctcatcat tttcagcaga ttttaaaggc ttacctacta acaacctttt
    15061 taagtaatcc aatttctaaa ctcctttaca cacaatttta cttcaagttt tatagtcgaa
    15121 aatttatagt aaacaagatg ttatagtacg ctcactgacc ttaaaagtca ataaacttta
    15181 agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc tttaatacag
    15241 caaaaatcta acattttttt atgggctttt tctttgaatt ctataataaa aggatagaac
    15301 ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa gagagatgta
    15361 aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt cacaacctct
    15421 tgataaacgg tgtttaaaag cttgcatctg cggtaataag cccaaccaaa ttaaaaacat
    15481 aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt ttttacaacc
    15541 tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa acggcaaaat
    15601 atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact gatttcacaa
    15661 cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt tcgtcatttt
    15721 ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa acaaaccgat
    15781 catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag attcccgtaa
    15841 caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga atttccctga
    15901 gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga tgataatagc
    15961 gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca tcatgaacgc
    16021 taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat ctactcctag
    16081 taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg tcaaaaatgt
    16141 cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt tctttcctaa
    16201 atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca gacatagtgc
    16261 agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg cattggtttg
    16321 tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt tcaataattt
    16381 gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg ctgctttttg
    16441 gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag cgttcacagc
    16501 tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga tttcattttt
    16561 tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt gctgaatgat
    16621 cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga tggtatccat
    16681 atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag gcatttcttt
    16741 ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg agctgagaag
    16801 cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc caatcgtatc
    16861 gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat tgataccagc
    16921 aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt catcaatagc
    16981 cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt gcagattgct
    17041 tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg tatttccagc
    17101 acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt gttgttgaga
    17161 tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat catcagtcag
    17221 cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg ttgttgccac
    17281 ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta tcgcatcttt
    17341 cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat tttctaaatt
    17401 tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt gttttgcttc
    17461 atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga tctctgctag
    17521 ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga tgatttttgc
    17581 ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac ccatctcatc
    17641 tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct tgatttcagg
    17701 tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt ccaaattttc
    17761 atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg tatcgatgtc
    17821 gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga taaattcatt
    17881 tgtgatttgc gattggatcg aacttgctcc tttatcgttg attttcggtg caatcgcatt
    17941 gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac tagtaaaact
    18001 cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact ttccggaacg
    18061 gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt gtttattttt
    18121 atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa actctgctgg
    18181 cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg gatcccataa
    18241 tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga tgatcagaag
    18301 tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc gaagcgtatt
    18361 tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac tttaccatgt
    18421 atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttt
//
LOCUS       DAFFSV010000048        18425 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFFSV010000048 DAFFSV010000000
VERSION     DAFFSV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.048"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            172..516
                     /locus_tag="LR110_001502"
     CDS             172..516
                     /locus_tag="LR110_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBL4822468.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="LR110_001503"
                     /pseudo
     CDS             517..936
                     /locus_tag="LR110_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="LR110_001504"
     CDS             complement(1028..1378)
                     /locus_tag="LR110_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822469.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="LR110_001505"
     CDS             complement(1344..2675)
                     /locus_tag="LR110_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBL4822470.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="LR110_001506"
     CDS             2931..3497
                     /locus_tag="LR110_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4822471.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="LR110_001507"
     tRNA            3642..3729
                     /locus_tag="LR110_001507"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="LR110_001508"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="LR110_001508"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL4822472.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="LR110_001509"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="LR110_001509"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBL4822473.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="LR110_001510"
     CDS             complement(7452..7898)
                     /locus_tag="LR110_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL4822474.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="LR110_001511"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="LR110_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL4822475.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="LR110_001512"
     CDS             8363..8881
                     /locus_tag="LR110_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL4822476.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="LR110_001513"
     CDS             complement(8958..9914)
                     /locus_tag="LR110_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4822477.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="LR110_001514"
     CDS             10080..10886
                     /locus_tag="LR110_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822478.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="LR110_001515"
     CDS             10883..11872
                     /locus_tag="LR110_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL4822479.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="LR110_001516"
     CDS             11869..12873
                     /locus_tag="LR110_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL4822480.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="LR110_001517"
     CDS             13005..13547
                     /locus_tag="LR110_001517"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBL4822481.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="LR110_001518"
     CDS             13567..14265
                     /locus_tag="LR110_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBL4822482.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="LR110_001519"
     CDS             14286..14498
                     /locus_tag="LR110_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBL4822483.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="LR110_001520"
     CDS             14498..15460
                     /locus_tag="LR110_001520"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBL4822484.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="LR110_001521"
     CDS             15478..15873
                     /locus_tag="LR110_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL4822485.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="LR110_001522"
     CDS             16036..16401
                     /locus_tag="LR110_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBL4822486.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="LR110_001523"
     CDS             16398..18209
                     /locus_tag="LR110_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBL4822487.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18417)
                     /locus_tag="LR110_001524"
     CDS             complement(18271..18417)
                     /locus_tag="LR110_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBL4822488.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
BASE COUNT         5920 a         3121 c         3809 g         5575 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggacgggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgcat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaatg
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agata
//
LOCUS       DAFFSV010000049        18292 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFFSV010000049 DAFFSV010000000
VERSION     DAFFSV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.049"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            71..3763
                     /gene="nifJ"
                     /locus_tag="LR110_001525"
     CDS             71..3763
                     /gene="nifJ"
                     /locus_tag="LR110_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBL4822489.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            3952..4839
                     /locus_tag="LR110_001526"
     CDS             3952..4839
                     /locus_tag="LR110_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBL4822490.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            4836..6005
                     /locus_tag="LR110_001527"
     CDS             4836..6005
                     /locus_tag="LR110_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822491.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6029..7384
                     /locus_tag="LR110_001528"
     CDS             6029..7384
                     /locus_tag="LR110_001528"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBL4822492.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(7456..9261)
                     /gene="glmS"
                     /locus_tag="LR110_001529"
     CDS             complement(7456..9261)
                     /gene="glmS"
                     /locus_tag="LR110_001529"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBL4822493.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            9670..9960
                     /locus_tag="LR110_001530"
     CDS             9670..9960
                     /locus_tag="LR110_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822494.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            9973..10998
                     /gene="sppA"
                     /locus_tag="LR110_001531"
     CDS             9973..10998
                     /gene="sppA"
                     /locus_tag="LR110_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL4822495.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11001..11660
                     /locus_tag="LR110_001532"
     CDS             11001..11660
                     /locus_tag="LR110_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL4822496.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            11817..12749
                     /locus_tag="LR110_001533"
     CDS             11817..12749
                     /locus_tag="LR110_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822497.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            12903..14192
                     /locus_tag="LR110_001534"
     CDS             12903..14192
                     /locus_tag="LR110_001534"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBL4822498.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVIIAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            14439..15689
                     /gene="clpX"
                     /locus_tag="LR110_001535"
     CDS             14439..15689
                     /gene="clpX"
                     /locus_tag="LR110_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBL4822499.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            15772..16359
                     /locus_tag="LR110_001536"
     CDS             15772..16359
                     /locus_tag="LR110_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBL4822500.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(16433..16588)
                     /locus_tag="LR110_001537"
     CDS             complement(16433..16588)
                     /locus_tag="LR110_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822501.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            16724..18052
                     /locus_tag="LR110_001538"
     CDS             16724..18052
                     /locus_tag="LR110_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822502.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            18063..18272
                     /locus_tag="LR110_001539"
     CDS             18063..18272
                     /locus_tag="LR110_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822503.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         6030 a         3246 c         3973 g         5043 t
ORIGIN      
        1 aaaaaaatgg catgttttac gttataatgg aacagataat aagagagaat atgaacaagg
       61 atgatgaatc atgcgaaaaa tgaaaacaat ggatggcaat acggcagccg catatatttc
      121 ttatgcattt accgagattg ctgcaatata tccaataaca cccagttcga ccatggctga
      181 attagtagat caatggtcag cagcaggaaa agaaaacgta ttcggtcaac cagtaaaagt
      241 agtagaaatg caatccgaag caggagcagc tggtgtggtc catggatcat tgaaaactgg
      301 tgcactgaca actacatata ccgcttcaca agggctgctt ttgatgatac cgaatatgta
      361 caagattgct ggggaactac tgccaagtgt tttccacgtt gctagtcggg cagtgacgac
      421 aaatgcgttg aatattttcg gagatcacgg agacgtgatg gctgcaagac aaacaggatt
      481 tgctatgtta gctgaaagca gtgtacaaga agtgatggat ttagcacctg ttgcccatct
      541 tgcttctatt gaaggagaag tccctttttt gaatttcttt gatggattcc gtacgagtca
      601 cgagattcaa aaaatcgaag ttttggaata tgaagaatta gctcctctga tcaatcagga
      661 gaaattagct tcatttcgta aacgagggat gaatcccaat catccgactg ttagtggtac
      721 gaaccagaat ccagacatcc attttcaaca acgggaaacg atcaacccct attatgataa
      781 attacctgaa atcgttcaaa aatatatgaa agagatcaac cgattacgag gaacttctta
      841 tgacttggtg acttattacg gagcagacga tgcggaagaa gtcattgttt ctatgggatc
      901 agttgcacag acgattgaac aaacgattga ttacttgaat gcaaacggac ggaaaacagg
      961 ttttttgaat atccatcttt atcgtccttt tccagtggaa acattcgtgc aaaagttgcc
     1021 tcatacagta aaaacaattg ctgttcttga tcgaagtaaa gaaccagggg caggtggaga
     1081 acctctctta ttagacgttc aaagtgcgtt atatgattct gaacttcgcc cggctgttat
     1141 tggtggtcgc tacggattgg ggtctaaaga tgtgactccc gaccaaatct cagcagtttt
     1201 tgatgaattg aaaaaagacc caagcatacg caaaaaacgg tttaccatag ggatcgtgga
     1261 tgatgtgact tatcaatctt tggaaccaaa ggaaagtctg gatttgacag aaccgcaaac
     1321 attccaggcc aagttttggg gatttggatc tgacggaaca gttggagcaa ataaatcagc
     1381 tatcaagatt attggagatc acaccgataa atatgcgcaa ggctattttt attatgattc
     1441 gaaaaaatct ggcggtctga ctgtctctca tcttcgtttt ggagatacgc cgatacgttc
     1501 agcgtatctt gtcgagcatg cggaccttgt tgcctgtcac acaccagctt acctgcatag
     1561 ctatgacttg gtcaaaggat tgaagccagg tggcattttt ttattgaata ctctttggag
     1621 cgacgaacaa ttagaaacgc atctgccctt aaaattaaaa cgttacttgg ctgaaaataa
     1681 cattagattc tatacgatca atgccatgag attggcccaa gaagtcggtt tgggacgccg
     1741 gatcaatacg gcaatggaaa cggctttttt taaattagct gacatcattc cttttgatga
     1801 ggtgcttccg ctgctaaaag aagaagccct gaaaagctat ggacataaat caatggctat
     1861 cgtagaaaaa aacatacaag caatcgatcg gacgaccgaa ctgcttcatc aagtcgcgat
     1921 cccagaccat tggagaacgt tggagatgcc tgaaaaaatc aataaaagga acactggtta
     1981 tatacaagaa atacttgaac cagtgaatgc acaggaagga aatcaactct ctgttggtac
     2041 acttgtagca aatggaatga caactggcga aatgccttta ggaacggctt ctatggaaaa
     2101 gcggggcgtc gctttagaag tacctgagtg gattagtgat cgctgtacga tgtgtaatga
     2161 atgtgccttt gtttgtcctc atgctgccat ccgtccattt ttagcagatg aagaggaaat
     2221 ggaagaagcg ccagaaggat ttatcgtgag agaaatgcga ggcgcagacg gcgtaaaata
     2281 ccgtatccaa gtttcagtgg aagactgtac aggttgtggt ctgtgtgtag aggcttgtcc
     2341 cgcaaaagaa aaagctttag tgatgaagcc ttatgaagaa cagaaagaac aagctgtgaa
     2401 ttgggctttt gcaatgacat taagacaaaa agcaaatcca gtcaaaggaa agaatactgt
     2461 cttgtctaca caattcaatc aaccgcttct tgaattctcg ggcgcttgct caggctgtgg
     2521 cgaaacacct tatgtaaaat tattgacaca aatgttcggt gatcgaatga tgatcgcgaa
     2581 tgcgacaggc tgttcttcta tttggggcgg tgcttctcct gcttctcctt atacgaccaa
     2641 tgaatgtggt caagggcctg catggtcaaa ttctttattg gaagataatg ctgaatttgg
     2701 ttatggtatg cttctagcgg cacagactcg tcgcgaggct ttggcaagtt caatcaaaga
     2761 agtattgcca aaagtatcag aagaactacg agaattgatg gaagactggt tagtacacat
     2821 gtttgagagt gaaggaacgc agcaaagagc agcaaaactg caagctgcga tcgaaagcga
     2881 acgatcaatt ttccctgaac tggaggatat atacaagaga aaggatctat tcgtaaaaaa
     2941 cagccaatgg atgattggtg gagacggatg ggcttacgac attggttttg gcgggatcga
     3001 tcatgtactt gccagcggag cagacgtgaa tatccttgtt ttggataacg aagtctattc
     3061 caatactggc ggtcaaacct caaaagcaac accagcttcg gcaatcgcta aattttcggc
     3121 tagtggaaag tatgcttcaa agaaagatct aggaatgatg gcgatgacat atggaaatgt
     3181 ttatgtggcg caaattgctt caggtgccaa tcaaatgcaa acgatcaagg catttgaaga
     3241 agcagaaaag tatcctggtc cttctctgat tattgcctat acgccttgta tcacacatgg
     3301 cttgcttggc ggaatgaaag aatcgcttaa agaagcaaaa gaagctgttt cttctggtta
     3361 ttggtcatta tatcgatata accctacaat ggtcgaaaaa aacaagaacc caatgacttt
     3421 ggactacaaa aaacctgatt tctctgctat gcctgatttc atgaggaaac aagttcgctt
     3481 ttcctcgcta gataacgcaa atccaaatct tgcaaaagct ttatttgaaa agactgtcca
     3541 cgacgctaaa acacgttttt acaactatgc tgcactgacg ggagaattag aaaaaatcaa
     3601 gaaacgactt gaaccagagg aatctatcga tgtacccgtt aagactagaa aagaaaaaac
     3661 gacggacctt gaagcagaag aaagaagagc tgctagaaga gctgccagag cagaacgacg
     3721 taaaaaaatg gaacaagcta caaaagaaaa aaaggaaaaa taacagaaaa aatataatga
     3781 ataattctct gaagtttaaa aatatttatt gcagagaaaa ataaaacgca tacttgcaat
     3841 tttcttgaaa attatgcgtt tttctcaaga aaaatacgag catatcagcc tctaaaatta
     3901 aaacatatga tatactagag aggaaatctc aaaagagaag agaggctttt tatgtcattt
     3961 caaatcgggg aactttttaa tttggactat tggcagcaaa tgttttccgg tgacttgttc
     4021 tctctgaatt ttttggtgaa catacttgat attctggttg tctggtatct tgtatacaaa
     4081 ctgatccaac tgctgcgagg cacaaaagcg attcagcttc tgaaaggtgt agctattttt
     4141 attgttatcc gaatcgtagc ggaaatcatt ggtctgcaca ccttgtcttg gctgatgaac
     4201 caagtcatta cgtacggggt gatagctgct atcgtgattt ttcagccgga aatccggaga
     4261 ggactggaac atcttggacg cagttcgctt ttcaaacaat caaaaagcga acagcaggaa
     4321 gatgagaaga tgatcttgtc ttttgacaaa gccatccaat atatgtccaa gcgaaaaatc
     4381 ggtgcgttga tcactatcga acgtcatact ggattagatg agtatattga gacagggatt
     4441 gctttagatg cagatatcac tggggagctg ctgatcaata tctttattcc taatacaccg
     4501 ctgcatgacg gtgcagttat tgtaaaagaa ggaaagatcg cagtagcaag cgcctatctt
     4561 cctctatcag aaagtatgtt gatccctaaa gaattcggga ctcgacaccg ggcagctgtc
     4621 ggtataagcg aagtcagtga cgcgattacc atcgttgtct ctgaagaaac cggggacgtc
     4681 agcatcacgt tagataatga actgatggct ggcttatccc agcaagaata tttggcaatc
     4741 ttgcgtagag aactaattgc tgaagagaaa acaaaaaaca agaaaaacgt gattcaatct
     4801 ttcctagacg gtatgactaa gggaggggat cgcaaatgat ctcaaaagag aaacggacaa
     4861 gcattcttta cagttttatt gcattgctgt tcagtattgc gctgtacttc aatgcaaatg
     4921 gccagtccgt tccaaatacg ctttctggaa atgaagcgta taatcaaaca gttacggggg
     4981 tgccgatcaa gatttcttat gattcaaagc gctactatat ccatggatat gaaaatgcag
     5041 tgactgtcaa actttccagt gcaaaccgag ttcagttgaa tgcagaggca aacgaagata
     5101 cacggatgtt ccgcgtcaca gcagatatca ctaagctggg cgaaggaaca catgaagttc
     5161 cgctaaaaat tcaaaatttg agcagtgctg tcacagcaaa gatcgaacca tcgactatta
     5221 cagtaacggt tgagaaaaag gtaataaaag atttcgatgt ggaaacgttg attccttcaa
     5281 cgttcacacc atctggatat gaacttgatg atacttccgt tagtcctaaa aaagtctcta
     5341 tcactacagg tgacaaaaca ttggcagaaa tcaaacgagt agtagctgat gtggacgctt
     5401 cgatggtcac agatgatggg attaatagtg aggtcccaat acaggcgctg aatgcagcag
     5461 gggaagtatt aagtatcgtc tctgatccag tgcaagtaac agtgagagct gattcgatca
     5521 agccaagcaa gtcggtacga ctttatggga tacagcaagg aacaccagct gccggggtag
     5581 aaagctatga tttcagtttt tcaaaactgg aagcagaagt aagcggctct agcgatttgc
     5641 ttgcatcgat cggtgacagt attgctgtac cgatcaatgt ctcaggtatc aatcatcgaa
     5701 caactcgtaa aattgaaata ccagtagaag aggggttaag tatcaagccc gaatcagtta
     5761 gtgtggagat tacacccgtg ttgcaaacgt caagcagtag ctctgcttcg acgagcagtc
     5821 ggacacaaga aaatacaagt acgagtagaa gtatttcatc tgctgattca ggaacggaac
     5881 catcgacaac aaccatttct tccagttcgg aatctgggac aagcagctcg accacaagta
     5941 cagaagaaac atcagaaaca agcgaatcaa cacaaatgag cagaacaaat tcctcagaaa
     6001 attaagttaa aggagtaatt ttacaactat gggtaaatat tttggaacag acggcgtacg
     6061 cggtgaagcc aacaaggaac ttacgccaga acttgcattt aagctgggac ggtgcggagg
     6121 ttatgtccta agccagcatg aagaagaagg aaaacgccca cgcgtgttag tagggcggga
     6181 tacaagaatc tcaggtcaaa tgttagaaga agcgctgatt tcaggacttt tatcggtggg
     6241 tatcgaagtg ttccagctag gtgtgatctc tacaccaggt gttgcttatc tgacacgttt
     6301 gcaaaaagca agcgcagggg tcatgatttc tgcttctcat aatccagcac aagataatgg
     6361 aatcaaattt ttcggtgcag atggttttaa actagtagat gaccaagagc tagaaattga
     6421 agcattatta gatgctgaag tggatgaatt gcctcgtcca tcagctgaag gattgggaac
     6481 tgtagaagaa tttccagaag gcttattgaa atattctcaa ttccttgtac aaacgatcaa
     6541 cggtgacctt tccggtttga ccatttgtgt cgatgcagca aacggagcaa cttcaacaag
     6601 tgttaatcgt ttgtttgccg atcttgaaac agatttttat acaatgggca caaatccaga
     6661 cgggttaaat atcaatgacg gcgtaggctc tacccatcca gaacgtttag ctgagatggt
     6721 cgttgaaaaa ggagcagatg ctggactagc tttcgatggc gatggcgatc gtattattgc
     6781 ggtggatgaa cttggaaaca ttgtcgacgg tgataaaatc atgtttatct gtgctaaata
     6841 cttggcagaa aaaaatcgat tgaaaaaaga tacgatcgtt acgactgtca tgagtaactt
     6901 aggattccac aaagcggtgg aaggaatcgg tttgaaagat gtcgttacac aagttggtga
     6961 tcgttacgtt gtagaagaaa tgcgcaaaaa cgattataac tttggcggcg agcagtctgg
     7021 gcacatgatt ttcttagatt ttaacacaac tggagatggg atgctttcag gaattcagtt
     7081 actgaatatc atgaaacaga caggtaagaa attatcagag cttgcagcag aggtaacaat
     7141 ctatccgcaa aaattagtga atatccgcgt aagtgataaa aacggtgcga tggatgttcc
     7201 ggcaatcaaa aaagtgattg aagaggcaga agctgaaatg ggatcagaag gaagaatcct
     7261 agttcgtcct tccggtacag aaccattact tcgagtgatg gcagaagcgc ctactgatga
     7321 gaaagttaat tactatgtcg ataaaatcgc ggcagtagtc aaagaagaaa tcggactgga
     7381 ttaagagata ttttcataaa aaataaaaga acaaggacca gtgtcagatg tctgacactg
     7441 gtccttgttc ttttattatt ctactgtcac tgatttcgct aaattacgtg gtttgtcaac
     7501 gtcgaatccc cgtaatagcg ttgcgtaata tgcaagtaat tgagtcggta cgactgtggt
     7561 tagagtagtt agataagggt ggacagttgg tagcaccagc tggtcacttt ccttttctaa
     7621 tccctctgag acaataacta gtgttttagc gccacggctt tcgacttctt tcaggtttcc
     7681 tcgagtatgc gcaccagtca cttcttcact gatgattccg atcactggtg ttccttcttc
     7741 gatcaaagca atcgttccat gtttcaattc gccagcggca aaaccttctg cctggatata
     7801 actgatttct ttcaatttca aagcagcttc aagagaaacg ttgtagtcag caccacgtcc
     7861 aatgtaaaag gcatttcttg tatcgctgag atatacttct gccaactctt ctaaataatc
     7921 tttttgggcg atgaccgatt ccatcgcatt agcaactaaa ctcaattcat gagtgatatc
     7981 gaatacaaga gaagtttcaa attctttttt atcaccgata gctttggcta ataaagttag
     8041 tacagctatt tgtgcagtat aagctttcgt tgatgctact gcgatttcgg gtccagcatg
     8101 caatagcagt gtaaaggatg cttcccgcga aagagtagaa ccagcaacat ttgtaatcgt
     8161 aagtgaaggg tagccgagac ggttgatttt taccagcact tggcggctgt ctgctgtttc
     8221 gccactttga ctcaagaaaa taaagaatgg tttttttgaa agaagaggtg tgttatagcc
     8281 aaattcactg gctaaatgaa cctcaacagg aatctgtgtc aatttttcca aagtctgttt
     8341 acctgctaac cccgcatggt aactagttcc gcaagcaacg atatagattc gatcgctatc
     8401 agacaatgta tcgaccagct tttgatcaag agagacacga ttgtcttctg ttacgtattt
     8461 ttgagcgata cgacgcatga taataggctg ttcgtcgatt tctttcagca tataaaaagg
     8521 ataggtacct ttctcgatat ctgaagcatc tacttgtgct tcgaatgaag aacgttctac
     8581 gatgtcacct gcaaaatttt gaataataac tttttctgat gtgacggtaa ccatctcttc
     8641 atcttcaatc tcaacgaatt ccttcgtatg agcaagcata gccattgcat cgctggcaat
     8701 cacattgaat ccatcgccga gtccgattaa tagtggactt ttatttttag cgacgtaaat
     8761 aacatcaggt gctgtccgat caatcaaagc gaaagcgtaa gaacctttga tgacccgcaa
     8821 agcttttaaa aacgcctctt gcgttgtagt atccttcgcg aaagtttcaa ttagatgagc
     8881 gataatctca gtatcggtct caccaataaa gtggtcattt gctaaataag caattttaag
     8941 ttcttcaaag ttttcgatta cgccgttgtg aacgagcacg aagcgtccac tttgggaagt
     9001 atgtggatgg gcgttttctt cagaaggttg cccgtgagtt gcccatcgtg tatgaccaat
     9061 acctatattt cctgtagtat caatatttat tttgtttttc aaattttgaa ttctgccttg
     9121 tgctttgacc agataatcag ttgtgccatc tgagacaaag ataccagctg aatcataacc
     9181 acggtattct aatttttcta gaccgtcaat caatcctggt gttacatttt ttaatccaat
     9241 cattccaaca attccgcaca taaaaaacat cctactttct ataatgaatt cgtatgaatg
     9301 cttccagctg caggaaaatc cttttgtttt atctttgcag acattttttg tggattttct
     9361 gtagagtgca gtccctaggg ccatccgccg aatctttcga taaaccctct cctcgtcatc
     9421 tgtttacaca gacctggcgc tatcctaaaa ggtataacta tcctcctttt tgttttttat
     9481 ctggaaacat ttaaaatcat aaccttttgc gaatgttctt gtcaacaaaa aaagaataaa
     9541 aaataaattg gtatatatca aattctttgt caaatgtcct ttttatgtca aaaaataatg
     9601 ggtttttatc tatcatcttt ttcaggagtt cggtacaata gaaagtaatc ggatagagga
     9661 ggagtaaaaa tggattcaaa aattgagatc atgacactgg gaatgttaaa aaaacaattg
     9721 tccaaattcg aagcatcagc tggtgtatca gatgatacga aaatattttt agatactggc
     9781 tgggatagca tccaagaaat cgcacctgat gccttggaag ttgctcaagc tcgtgagttt
     9841 acggtagaag atgaattaac aaaagagtcg ttttccgggt atgcgcggga agaaaaagca
     9901 gagcggttcg acacttctga gcagtcagaa actgtgatcg tcataaaaaa cttgtattag
     9961 aaaggagaaa gaatgaataa aagacgttgg gcagcagttc tgattgctgc aggattattt
    10021 gtggtttcgt tagtttcagc tagtttgact aagcctcagg aaaacgatca aattgaggga
    10081 ttgaacagtt ggttatatgg ggatgaagaa ctagctccaa atgtgttgga agagggagac
    10141 agtacgcaaa aaatcgtaaa attatcagta gatggaacga ttgctgcggg agggtcttca
    10201 ggtttatttg cttctgaagg ttacaatcat aaaaatttta tggaacaact gaaggcgatt
    10261 gaagaagatc caacagtcaa aggtatcttt ttagaagtaa attctcctgg tggcggtgtc
    10321 tacgaaagtg cggaaatcgc taagaaattg gatacgattc gtaaagaaca tgatatccca
    10381 atgtatgtca gcatgaaaaa catggcagct agcggtggct actatatttc tgctcaagca
    10441 gataaaatct ttgcaacaga agaaacagtg accggctcaa ttggtgtgat catgtcagga
    10501 ttgaattatt caggacttct agaaaaactg ggtgtggaag atacaacagt taaaagcgga
    10561 gcgttgaaag atatgggttc aggaacgcga ccagagacaa aagaagaaga agcggtgctt
    10621 caagcatata tcgacagcgc atatagtcgt tttgtagcaa tcgtcagcga gggacggaat
    10681 aaatctgaag aagaagtcaa aaaaattgct gatggaagga tttatgatgg tgtccaagca
    10741 aaagaagtag gtttagtcga tgaattggga tttcctgaag atgctttaaa agctatacga
    10801 aaggaacaac agcttgaaga tgcggaacta gtagaatatt cttccacgcc atctgggttt
    10861 gctagtacat ggttaggaag taaactagca gaattccagg gattgaagcc ttctgagact
    10921 agtcagatct tatccgttct tgagaatcta gggacagcgg aatcaccaaa agcaatgtat
    10981 tattacggag gtgagtaagc atgtcagagc tttcaaatga aaataaggaa caagaaaaag
    11041 ataagcaaca gcagcctgct gtaggacctg actccaactt ttcccaaaag gtactgagct
    11101 cttttgaaag agagcctttg acgcccgaag aaatcaaaaa gaaacaacgt aaatggaaaa
    11161 agtatacgga cacaagaaat gaaccgccaa aagcaaatga ttttcctaag tatttctatg
    11221 caggtttttt tatccgaatg tttgccttct tggtcgatct tttgtgtatc ggagctatta
    11281 cgcgtttaac acttggtatt gccaaaaatc taggttggct taatttttca gattcttatc
    11341 tgagtctcta cggattcaca gccttactta tttatctagc gtattttatt ttattgacga
    11401 aattcaatca tggccaaacg attgggaaaa tgatctttgg tattcgagtc atcagctttg
    11461 atgaagaaga acttagctgg tcaaccgttt tagttagaga aggtgcttgc cggttcattc
    11521 taaaatttcc tttgctgatg ttagggtatc ttccagcagt ctttaatcag aaaaagcaac
    11581 atctgggcga ttttttcact aatacaagtg ttgttacgat caatctgatc aaagcgttca
    11641 accaggaagt caacagctga tgagcaataa aaagcagtaa cagttgaaag caccttctaa
    11701 aagtcagatc taaaatctag cttttagagg gtgcttcagt tatttttaat tgtcatgctt
    11761 tttttgtttt gctactattg aacatgagct gaatatgagc aagaggagga cactgcgtga
    11821 agccgattga ttttccagaa aaatatgaga atttaatgcg agtggcacaa caagcactag
    11881 caaaccagca atatcagcag gcaaaagaat tatttcaacg agcttacgag ttgaaagaaa
    11941 gctttgaggc taatagcctg cttgtttttt gtctttacga gttggacgag aaaaaagaag
    12001 cattgaagca agcactcttg catgaaaaac agtatcttca aaatgaggag tttgctgaat
    12061 tttattttga cttgttaatc agtacacaag attttttata tgctagaaaa ttgatagcaa
    12121 gtacagattt ttatgaagct ttcgagcagc gggtcattga aaaaatccaa tttgcagaag
    12181 aattatccgg acaaatggag aggcagaaga tcaaggcact ccacaataaa tcggaggaac
    12241 ttccttcgtt ggaacctgca cgtcagcttg cgtctattga tgagatcgaa cagcttcctt
    12301 atcatgagtt tatccaaaca gcgagtaaac ttattgtatc gccagaggtc catattttag
    12361 caagggcaaa attactggag acacttaggc aattgaacga atgccatcct gttttctatt
    12421 tgacgattga agaaaagtta gtcaaagtca taccaaaaga tctgccaaag ccgcagcagc
    12481 aggcaagtta ccagcaatta tgtgtatttt cagatcgtta cgggaatgaa gatgcgctgc
    12541 ttagttccgt gttaaaagaa gaattcacgt tgcaatctgc aattgtttat cctgtctatg
    12601 atacatacat cgaagacccg aagcgctggt ttcaactgac tgtagaggcg tgtacaggaa
    12661 aaacgatgta tgaaggtaca gaagatgaaa aacaagattt tttaaaaaaa agagaaaaaa
    12721 ttttgcagca aatgatcttc ttccattaga ttttatcagt gaaaaagata aaaatgttat
    12781 gaaaaacaag gattctatcc attcaatatg tttacaattt gaaagggtta gtgtactatt
    12841 taaaggtatg catgaaacgt ttttgatgta taaaatgaat ttctaaactc ggagggaatt
    12901 aaatgactgc aacatttgaa aaaaaaggca cgaatgatgg tgttttaaaa tttacgattg
    12961 cgcaagacga aatccaaaaa ggattaacaa ctgcgtttaa taaagtgaaa aaaagcttga
    13021 acgtacctgg attccgtaaa gggaaagttt ctcgtcaagt attcaaccgt atgtacggtg
    13081 aagaagcttt atatgaagat gcattgaatg ctgtattacc agaagcttat gaagctgctg
    13141 tcaaagaagc tggcatcgat cctgtttctc aaccaaaaat cgatgttgaa agcatggaaa
    13201 aaggtcaaga ctgggtaatc attgctgaag taacagtgaa accagaagta aaattaggac
    13261 aatataaaga tctagttgtt gaaaaacaag accgtgaagt aacagatgct gacgtggatg
    13321 cacgtatcaa acgtgaacaa gaagcacaag ctgaattagt tatcaaagaa gatgctgcag
    13381 ctgaaaatgg cgatacagta gtaatcgact tcgaaggctt tgtagatggc gaagcgtttg
    13441 atggcggaaa aggcgaaaat tactcacttg aattaggttc aggctcattc attccaggtt
    13501 ttgaagatca attagttggt cataaatcag gtgaaagtct agatgtaaat gtaactttcc
    13561 cagaagatta tcaagcagaa gatcttgctg gaaaagaagc agtcttcaaa gtgacaatcc
    13621 atgaagtaaa agcaaaagaa gtgcctgaat tagatgacga attcgcaaaa gatgttgatg
    13681 acgaagtatc aacattagac gaattgaaag aaaaatatcg caaagaatta gtagaaacaa
    13741 aagaaaaagc agcggacgat gcaaaagatg aagcagcgat ccgtatggct gttgaaaacg
    13801 cagaaatcgt tgaattgcca cacgtgatgg ttcatgatga agtacatcgt tcaatggacg
    13861 aattcttgaa caacatgcaa cgtcaaggta tctctcctga aatgtactat caattaactg
    13921 gttctacaga agaagacttg cataaacaat tcgaaggtga agcagaaatg cgcacaagaa
    13981 caaatcttgt gatcgaagca attgcagctg ctgaaaatct tcaagcaaca gaagaagaaa
    14041 tcaatgcaga aatccaagaa ttatcagaaa catacaacat gccaatcgaa caaatcaaac
    14101 gcgtgttgac agaagatatg ctggaacatg atgtgacaat gaaaaaagca atcgaattga
    14161 tcacaggtac agcgacagaa gcgaaagaat agaaatcaag taataaatca tttctgaaga
    14221 agctacttct agaaaattgt ttattcgaaa taagggactg tgacacgatt ttgtcacagt
    14281 tccttatttt ttgttaagag taaaaaatgc aaaggtatag gtcagttgat cctttgttaa
    14341 acactttttc tttttactaa actatgttat gatagtaaaa gactgtaaag tctttgatag
    14401 tttcttgcat actttttgca agttaagggg tgaagaccat gtatgacaat ccaagtagca
    14461 atgagaccgt acgctgttct ttttgcggga aaacccaaga agaagtgaaa aagatcgtag
    14521 ctggtccagg ggtctacatc tgtaatgaat gtatcgacct atgtaaagaa atcattgatg
    14581 aagaatttta tgaagaagct gtacgcgaat tcactgaagt ccctaaacca ctagaaattc
    14641 ttgaagtatt gaataactac gtgattggac aagatcgcgc aaaacgctcg ttagctgttg
    14701 ccgtatacaa tcactataaa cgtgtcaatc agcaagcaca agaaaacaca gatgatgttg
    14761 aactgcaaaa aagcaatatc tgcttgatcg gacctacagg gtctggtaaa acatttttag
    14821 cacaaacatt agcaaaaaca ttgaatgtac cttttgcaat cgcagatgcg acaagtttga
    14881 cagaagctgg ttatgtgggg gaagatgtag aaaacatttt gcttaaattg cttcaatctg
    14941 ctgattacaa tgtggaactt gcagaaaaag ggatcatcta tattgatgag atcgataaaa
    15001 ttgcgcgtaa aagcgaaaat gtatctatta ctcgtgacgt ttccggcgaa ggtgtccaac
    15061 aagccttgct gaaaattcta gaaggaactg ttgcaagtgt gccacctcag ggcggacgta
    15121 aacatccaca tcaagaattt attcagattg atacgacgaa catattgttc atcgtaggtg
    15181 gtgcatttga tggcatcgaa acgattgtga agaatcgtat gggcgaaaag acgattggat
    15241 ttggtacaca aaaccaaaaa ctttctgaag acgaaagtgt catgcagcat atcattcctg
    15301 aagatttact aaaattcgga ttgatccctg agtttatcgg acgattacct gttatggcag
    15361 ctttagagaa attgacaaca gatgatctgg tacggatctt gactgagccg aaaaatgcgc
    15421 tagtcaaaca atatcaaaaa cttctttctc tagataatac cgaacttgaa ttcgaacctg
    15481 aagcgttgag agccattgcg aaaaaagcaa tcgaacgcaa taccggtgct cgaggacttc
    15541 gttccatcat tgaagaaatc atgatggatg tgatgtttga tatcccttca aatgaaaaca
    15601 tcgaaaaagt gattatcaca aaagaagcag ctgaattgtc tggtgagcca acagtaatct
    15661 ataaagattc agagaaaaaa gcgggttgat gaaaagaggc tgccgtaaaa gtcattttgg
    15721 cttttgcgac agccgcttgc tttattcatc accttgttaa ggagagagac catgaaagtc
    15781 catcaagctg aaattatcat aagtgcagtg gcaccaaaac aatatccaga tacacaattg
    15841 ccagaaatag ctctggcagg acgatcaaat gtagggaaat cttcttttat caacacattg
    15901 attgatcgaa agaatttagc ccgtacttcc ggtaagccag gaaagaccca gacattgaat
    15961 ttttatttga tcgagaacgc attacatttt gtcgatgttc cgggatatgg gtatgcaaaa
    16021 gtatccaaaa ctgagcgagc aaaatgggga aagatgatcg agacatattt aacgactaga
    16081 gaccagctac gtgcggtcgt ttctttagta gatttgcgtc ataagccttc tcaagaagat
    16141 atccagatgt atcaattttt gaagtattat gatatccctg taattgttgt tgccactaag
    16201 gcagataaga tccctcgtgg taaatggaac aagcatgaga gcgaaatcaa aaaagcgttg
    16261 gattttgatc gcaatgacac gtttattctc ttttcttccg ttacaaaaca gggaaaagaa
    16321 gaagcgtggg cagcaatcga acaagcaatt gaatcataaa aagaataaaa aagacttacc
    16381 agtcaaactc tttcataaaa gtttggctgg taagtctttt tttgagctta acttactttt
    16441 gtttcttgtc ttttttcttt tcattttgtg gctgtggttt tagaaatcga tcgatctctt
    16501 cttttggtgc aactgttttt tccggatcga cagcaaatga ttcgaaaagt ttatctaaat
    16561 tatctgtacg ttcaaatttc aaccccattg taaacacctc tttgtttctt tttacttagt
    16621 ctatcatgta atcgtccaaa ttggtttcta agacataaag ttttagtttt tctttatcat
    16681 tggttataat taaattataa aaagatttga aagaggtgag aaaatgattg gcgaagataa
    16741 attattgatg actatccagc tcacagactt aaatgaagaa gaaaaaaaac aggaaataag
    16801 cgattgggct attttgacac gtctgttgat ccagccaaca tttatccgct ctttcacaca
    16861 acaagcggat ccgtatgatc ttcgaaagct tcaagaaaaa ataatgctag catcggtaag
    16921 agacgaaagt tggttagttg taggaaatga cgagaacgaa tgtagttttc gcttggaaga
    16981 taatcaatta ttgataaaaa atgtattgtc tatctctgtt ttcaaagaaa agcaattttt
    17041 gattcgagac ttcattcaga aaaaaatgat tgctcatggg gtgttcgcct atatgcgagc
    17101 ttatagcgag tttatctatc acaatacaaa acagatctct caacggctat tgtttgagaa
    17161 aaaggatgaa atcgaacatt tacctaaaat gaaacaacaa aacggggaag tagtagtcga
    17221 ttgcaaccaa tttccaggat acgatctttt ttaccaaggt ttatgtttca cttcgtgttg
    17281 ggaaatgtat tatagtcgct attatcatca aatcatccct aaaccgattt ttctcgacat
    17341 ccaacaagta gaaaaagtga aggaattaga aaacgaagtc atatgtatcc agctttatcg
    17401 agaccccttt aactggcaaa agccaaacaa tcaaatgttt caatcctatt ttagggacca
    17461 gcttgggttt gatcatcttg cttgggacaa tggtgtcggt ttattgaaac cgccattcgt
    17521 tgaatacatt tacactgacc atacgatcca atctgtccag tatcagaatg cacaaatgca
    17581 acctgttcct aaaaagaatg cttcattttt tgtaacaaaa agttatgata tccaacaagg
    17641 agattataaa gaaaggcgtg ttcgcggaac actaaatgct caggcgtatt ttccttgggt
    17701 tgacgagaat cgatcacgca tgatgtgcta taaagttatc gatccgactg ttgctttgga
    17761 taatggaatc gaagcttatt gttattatat tagggaatat ctagaagtag aggtaacaga
    17821 tgaaaaatat caagagtatc ttttatcctt aagaatctat gttccttcag aatccctgtc
    17881 agaattaccg ttaaaggaaa tcaaacatag actctctgat gttaccttca aacgaataaa
    17941 gaaaaagcgg cgaagtatcc aattcgatgt caagaaaggt gagcagcatc tgcgtgtgca
    18001 atttttagat taccgagaat tagaacaatt gattacttta caaaaaatat gaggtgaaga
    18061 aaatggataa acaactctat tatcgaataa tggttccggc atcattatta tccgtctgtc
    18121 tcttttttgt tcctaatttt cctattcgat cagtcatcgg ttttttggtc atgtttggcg
    18181 ggatgggact ttattattat cttcttcata aggaaagaaa acctgcttct gtttatatga
    18241 aaaaaacaag aagcgaaaat aaaaaacgat aaaacggctt gatgatagac aa
//
LOCUS       DAFFSV010000050        18105 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFFSV010000050 DAFFSV010000000
VERSION     DAFFSV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.050"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            82..2142
                     /locus_tag="LR110_001540"
     CDS             82..2142
                     /locus_tag="LR110_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBL4822504.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2225..2794
                     /locus_tag="LR110_001541"
     CDS             2225..2794
                     /locus_tag="LR110_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4822505.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2969..3775)
                     /locus_tag="LR110_001542"
     CDS             complement(2969..3775)
                     /locus_tag="LR110_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822506.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3913..5427
                     /locus_tag="LR110_001543"
     CDS             3913..5427
                     /locus_tag="LR110_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBL4822507.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5830..6813)
                     /locus_tag="LR110_001544"
     CDS             complement(5830..6813)
                     /locus_tag="LR110_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBL4822508.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6818..7465)
                     /locus_tag="LR110_001545"
     CDS             complement(6818..7465)
                     /locus_tag="LR110_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL4822509.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7550..8182)
                     /locus_tag="LR110_001546"
     CDS             complement(7550..8182)
                     /locus_tag="LR110_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL4822510.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8198..8749)
                     /gene="coaC"
                     /locus_tag="LR110_001547"
     CDS             complement(8198..8749)
                     /gene="coaC"
                     /locus_tag="LR110_001547"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBL4822511.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8746..9483)
                     /locus_tag="LR110_001548"
     CDS             complement(8746..9483)
                     /locus_tag="LR110_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBL4822512.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9480..10469)
                     /locus_tag="LR110_001549"
     CDS             complement(9480..10469)
                     /locus_tag="LR110_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBL4822513.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10727..11269
                     /locus_tag="LR110_001550"
     CDS             10727..11269
                     /locus_tag="LR110_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4822514.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11286..12371
                     /locus_tag="LR110_001551"
     CDS             11286..12371
                     /locus_tag="LR110_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822515.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12383..13189
                     /locus_tag="LR110_001552"
     CDS             12383..13189
                     /locus_tag="LR110_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822516.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13190..14017
                     /locus_tag="LR110_001553"
     CDS             13190..14017
                     /locus_tag="LR110_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822517.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14014..15087
                     /locus_tag="LR110_001554"
     CDS             14014..15087
                     /locus_tag="LR110_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4822518.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15515..>18105
                     /locus_tag="LR110_001555"
     CDS             15515..>18105
                     /locus_tag="LR110_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL4822519.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYV"
BASE COUNT         5839 a         3210 c         3709 g         5347 t
ORIGIN      
        1 ttgattttat ttatataatg aaatcatctt atgaaaatta ctacttcatg tttagacaag
       61 agtagggagg actaagaata aatgtgttca aaaagacgtt caaaacaact aaataaacac
      121 gcttgtcgtt tagataagga acggtcgtgt tctgaaaaac actacgttaa gtttatgatg
      181 cgcacgatgc tgatcagttt atctgtaggt gtctatatgc atgtacccgt tcaggcaacg
      241 acacaaacca tcaaggtcgt acgtacctct gtttctgaga ccactgattc ccctatcact
      301 attactcata ctttcactga tgcaaatgga aaacagctac ttcctgcatt gacttcctat
      361 aataaagtca gttatcctcc aacgataaac cactactggt taattcctaa caaaacatta
      421 tggacatcgg aaaccggaag cagtgtgccg ctttctatct tttccgatat gactgatcct
      481 acaaaaggtg atcagttaaa acagatcact gatattttga atcatgcttt gacagatata
      541 aaggacggtg gaagcgcaca agtttcttat gtatatgaaa aagattattc acagtttgaa
      601 agaaagaaaa tcgatgttct ggcagggaca aaagtttcga tatctgatct ggtagaaagg
      661 ctgaagaacg cagatggaac acaaggagat atgtccaaag taatttcaaa caatgggaat
      721 atcgtggata cgactaatac aggaacatct actatttcct tgtcctattt tgatccaatt
      781 gctttaaaaa caatgactgc atctgctgtg atccatgttc gtgctgatca aacagctatc
      841 aaaggaaaag atcttcactt aagaacagga gagagttacg cccctaaaga tcttgttgaa
      901 tatgtaacaa aaagcgatgg aacttcagga aataaagaag aaatcaaaat gaacgggaca
      961 gtagatacta caactccggg agaatatccc gttgtattaa cttatataga ctcgaaaaca
     1021 tcaaatgtgg cagaaacaac agctgtagtt tatgtagaag catctgctgc ttcatcgact
     1081 gtcatcactc attttataga tagtcaaaca aataaagaac ttcaagcaga aacacatatg
     1141 agtagtgtaa atcgtattcc tgatttgcgc ctacctgctg aattttctga tcgagtgcca
     1201 aatatagatt tatcgactta tgaacaaaat gggaaagtca cttctatttc tgattggatg
     1261 caagcaaacg gattagacag aaaaagtcac tggtcggaga tccttgaagt acttcactca
     1321 gaactgatga ataataggaa gcaatcagga ggggaaatta cctttactta tgtttatata
     1381 ccagacaatc gttcattaga aggtcatgat cttattgttc atccaaacga aaaaataaac
     1441 aaagaagagt tgatccaatc agcaacaaat tccttaggta aacccgtacc gttaaaggat
     1501 gtatcggtta caatcaatga agtaccattg gaggatggtt atttgaccaa acaaacaggt
     1561 gattttacga ttatgtatag ttatcatgac ccgtattcac tccaagaact ccatgctcag
     1621 tcactattac atgttttacc attaaacaaa cagatagatc aaaaagaaca aaaaagacaa
     1681 gaagaaagct tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc aacagggaaa
     1741 gtaaaacaaa caagaagcct agagttagca agaatatcta gaaaaatctc agaatctaat
     1801 aaaacgataa ggcaagaagc tcatttggcc ccacaagttt ctggattgga aaatgaaatt
     1861 ttagagccta aaataaaaca acaatcagaa aaagaatttg ttgctaaaca gtcggaagat
     1921 cagaaggaat cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc
     1981 accgatgagc atctgcagcc gcaagtacca cttcctagtc cgggaacacc cgcacctggt
     2041 ggaggtattc cttcttctgc tttaggagat ttcatgcgag gtatggcagg aacatgggta
     2101 tacgctaatc gaaatgaatt tgacgcagat gactggttct gatttatatc tctatctgtt
     2161 atagtcaaat aagtagctga gtcatatgtt atttggcata atggagacga ggagggatag
     2221 aaaaatgtta gataaaacgt taccttacgc agaaatctgg atgacaagac cacttcaaca
     2281 aaatgttcca atcacgcctt tagcggaagg ttttgaattt cgattttatc aaaaagaaga
     2341 tgaatctgct tgggcggaga ttgagacttc cgttggggaa tttcaaacga cagatgaagc
     2401 acttgagtat tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt
     2461 ttttgtagag acaaaaaaag gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc
     2521 aagagagcaa gtgtatccgg tttttcattg gttagcagtg aaaccagatt accaaggaaa
     2581 agggctcgca aaagcattga ctgccagagt cttgcaagaa tttcctaccc tccatacgga
     2641 cggaccaatc tatcttcaca cacagacatg gagccatcag gcgattgctc tttataaaaa
     2701 aatgggtttt acttttattg ctgaaaatct agacggcaca cctaatccag attatgaaaa
     2761 agttatgcag gttttaagta aattagataa ataacgaaat ggtgaaataa gtcctcagta
     2821 ttgggataaa aaacgaaata gtcattcctc atatcttact tttttgagtt aatactatcg
     2881 gataactaat ggcatgccat acattcaaaa aagaaggggc agtgacaaaa gtattgtcac
     2941 tgccccttct cttgtttttt agcatgtttt atcgtttgat ttttttagga ataaaattct
     3001 tgatgaacgt aaatttttgg taatcgtttc gactatagag gtaaccaaat acactgaaaa
     3061 caactgctcc aataaaatta acaaataaat ccttcatagt atccatgatt cccacgtcta
     3121 agtaaccatt ttggatgatt gttttatctg ttttaccatc tgcattttga ctttcaatga
     3181 ttgtccgagt gatatctgga acatgataga cagtgtttcc gtctttactt aactccacac
     3241 tattgatttg ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat ttatctgctg
     3301 aaaattccac aaactcccac ataacaccaa ctgtcatcga aaaacagaag gaaacaactg
     3361 ctagtaataa aggagtcaag ttcatggttt ttgtattttt attgagcaaa taaacaaggg
     3421 aaaagccgac acccccagct aaaaatccgc tgaatgcatg tagaaaagta tcccacattg
     3481 aaaaatagcc ataaaaatca cttaactctc ctaaaactgt tgaactaaaa ataaaagata
     3541 acaaaatgat ttctagcata tttggaatct gtatctttaa tatcttttcc agaaaataag
     3601 ggatttcaaa taaaattaat gtcagtaata aaagcattgt atttccccag tgttggttcc
     3661 agacctgatc cactagcata ataacgacga tgatacgtaa taaaatatag attccgtttg
     3721 tgattctacc tgcagttttt gtactgttcg taaaaaaggt tttcaaatga ttcataaaat
     3781 aactccttca tgtatatcct taattatact aagaagcgtg tgtttaccaa agctatatga
     3841 cttaaagaaa aacagagttg atattgtgaa aaaaatcact taatatagta taaggagaaa
     3901 agggggatac aaatgaaaaa attagttgct ggtttattaa cagccgttgc acttcttgct
     3961 atgagcgcat gcggcgcaga tcagaaaaat acagcgcaga caagttctag tacgaaggaa
     4021 tctgccgaag tatcatcagg cgcttctgaa caagtatata cagatccaaa agaaatgaaa
     4081 gaatcttatg atgtcgtgat tattggagct ggtggtgcag ggatggcagc agctatcgaa
     4141 gcaaaagatg ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc cggaggaaac
     4201 acatctaaat catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa agaagaagga
     4261 atcaaagact cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg aggaacgaat
     4321 gataaagaaa tgcttcgata ctttgtcgat cactctgctg atgcggtcga ttggctagat
     4381 caaaacggta tcaaactgga caacttaacg atcactggag ggatgagtga aaagaggaca
     4441 catcgtccgt ctgatggctc ggctattggt ggatacttag tggaggggtt attgaataac
     4501 gtccatgagc gggaaatccc tatttttgtc aacgcagatg tgacagacat taagaaaaat
     4561 ggggaaacgg tcaatgaagt gactgtacat gttcaaggag aagaaccaaa agaggtcacc
     4621 ggaaaagcaa tcgtagttac cacaggcggt tttggtgcca gcaaagaatt tattgaaaaa
     4681 tatcgtcccg atttagctga ttatattacg acaaatcaag aaggatcaac tggagatggt
     4741 atcaccatga ttgaaaaatt aggtggacaa accgttgata tggataaaat acaaattcat
     4801 ccaactgtgc agcaggatga gggggtcttg attggtgagg ttgttcgcgg agaaggagca
     4861 atcttggtcg atcaagaggg gaaaagattt gtcaatgaaa tggatacaag ggataaagta
     4921 tctgcagcaa ttacagcatt gcctgaaaaa tcagcttatt tgatttttga ccaaggcgtg
     4981 cgtgatcgtg ccacagctat tgaattttat gaccaaaaag gatatgtcac aactggaaag
     5041 acaatggaag aattagcaga aaaaatcgat ttaccaaaag aaacactagc aaaaacagtt
     5101 gatggatgga atacggatgt tgcggagaaa aaagatgctc aattcaaccg tgccacagcc
     5161 atggaacatg acttatcgtc tccgaattat tatgcgatca aaatcgcacc aggaatccat
     5221 tacacgatgg gcggtgtgaa aatcaatacg aacactgaag tattagataa agaaaaccag
     5281 ccaattaggg ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg aaacaaccgg
     5341 atcggtggaa attcagttgc ggagatcatt atttttgggc gacaagcggg catacaagca
     5401 gctcgatttg cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg ttgtgaaatc
     5461 agcgttttga gctaataccg agggtcagct gattgaacac tgtttagtga agaggttgtg
     5521 aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt
     5581 taacttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta ttcggaaaaa
     5641 gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
     5701 gaagcagaag aaattccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt
     5761 tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttgatc
     5821 agcatgattt tattcagtta tcttcaaaat gatttttccg atgtttttgt ttgcttccat
     5881 atattcatgc gcttgtttga ctgcgctgaa ttggaatgta cggtcaatga ttggccgaat
     5941 cttgccattt tctagaaaag gcccagcttt ttgcataaaa gtttgtgcga gctctgcttt
     6001 atactgatca cttctaggtg tcaataatgt accaagcagt tggacacgtt tcccaatcaa
     6061 tacaccaagg tctatctcag gcacgatggt cccacctagc ataccaatca agacccaacg
     6121 tccatcaacg gctatagaag ctaaattctt ttcccagtaa gaagcgccga tgaaatccaa
     6181 aataacatcg actccccgac cttcagtgat ttttcgtacc tcttcttgaa agtcttgttt
     6241 tttatagttg atcaattcat ctgcaccaag ttctttacaa aaggcgagtt tttcttctga
     6301 tcctgcagta acgatgattt tcgcgtgaga aagctgtcta gctaattgga tcgctgctgt
     6361 tcccacacca cttgctcctg catggatcaa gacagtttcg ccttcttgta attttccaag
     6421 ccagtaaagt gtttgataag cagtcaaaaa aacttctgag attccagcag cagaaacata
     6481 gtctagcgta tcagggagaa ggattgcacg atcggcaggc atcacagcat attcggcata
     6541 accacctaga ttgactaatc cgtatacttt tgtcccagga gtaaaagtgg agatatcact
     6601 tttattctca actacctctc cagctacttc gactcccaaa atagggtagg gaggtttcaa
     6661 agagactgat tctcttttca taatatctgt tcgattgaca gctgctgtat ggactttgat
     6721 cagtaattcg ccttgttttg gtttaggaac aggcacttca atttgttcta atacatctac
     6781 agaaccagga tgcttgattg aaatggcacg catggatcta ttcctccttt gcccattctt
     6841 ggaatgctgt attcaactct ttgctacctt cagaaagtac tagagtcgta cgagaaatat
     6901 ccgtttcttc cactagagcg gcaagtacag cagcaactgt agagcgagtg atcgtttttg
     6961 atgccttctc attaggctta gttaattgaa tggaagtaac ttcttcatcg ttagtcaatg
     7021 tgactggttg aagaatgaca aaatctaagt tagttcgttt cagccattca tctgcataat
     7081 gtttagtaat ataataatct gtcatggact caggccattt tgctggttca tctgcataaa
     7141 cagcactgac catcagatag cgagagacat gcgcattttc agctgcgatc actgttttga
     7201 ttgcgccatc caagtctact tgcagaagat tctttccttg agagccggca gcaaaaatca
     7261 atacatcgat gcctttgaat gcttcggtca ttttttccac ggaccaagtt aaatcaaaag
     7321 agacatacga aacattctct ttttttacta atgtttggga agcaacatct ctcacccctg
     7381 caaaaatttc atgggaactt ggagctaaat cttttatcag gtgtcggcca atttgtccgt
     7441 ttgcacctac aacaaaaatt ttcatttgga tcatcctttc gacgtcaaat ctttccattc
     7501 aatagtaaca ccaaaagaga aaaaaatcag agatcggcta cgatgactat cagttagtaa
     7561 gagaagggtg cttgaagtat cttaacaaga cattgcatac tgctgcagtc gcaagacctg
     7621 cgacgattcc ttcaggtata ccacttgtaa aaataaccgc tagaatagct gaataaacag
     7681 catttaaatt tgttccgatg actttggcat aatcttgctg gaataaaaag tagatcatat
     7741 tcatcactaa aatcgtattg gtcaatgaac cggaaagtcc agctaagaat aaagaaacgg
     7801 aatctgcttt ttgtttcatt agtttgcgca gacctttata aacgaaataa ggaaccaccc
     7861 caatcaagat gcgaggaata aacgcgatca gcaaggcttt ccagcttcca tgttctgttc
     7921 cgatgactgg gataaacgga gaaaagacaa aagacaaagg tgtttggata actgtgctac
     7981 ggatcatgct gatcagtcca aatgtaccgc caagaaaagc acctagtctt ggtcctaaaa
     8041 cgatcgaagc aatgattaca ggaatatgca tggttgttgc attgattgga ccgataggga
     8101 taaaacctaa aggcgtaaca gctaataaaa tcaaaatagc taaaaacatc gcagttaatg
     8161 taaagttttt tgtatttttc ataagtactc cttttttcta agcatttctt tcattaagag
     8221 tcagcatgac ctgttcgatg atcgtctgaa tatcagctaa ggcacctttt ccaaaatctc
     8281 cacaagctaa aagagattct cttggttcaa tttcctgata gccgacagat ctcaatgttt
     8341 ccagattacg ctgagtgatc gggttttgat acatattcgt gttcatggca ggggcaatca
     8401 attttggtgt tcccactgga agcgccattg cgactgttga aagaagatcg tctgcaatgc
     8461 cgtttgctag cttgccgata atgtttgctg tagcaggcgc gatgagaaac aagtcagctt
     8521 gttttgctag ttcgatatga ttgattacct gcggatttga ttcttgcatg acgtctgtat
     8581 ggactgcgcg cttcgacaga gattgcaagg ttaatggcgt gataaaatgt gtgctgcttt
     8641 tagacataat cacatctacc tgggcaccta atttgacgaa ttgacttgta agatcagcgc
     8701 ttttgtaagc agaaatactt ccagtaacgc ctagtaagat ctttttcatt ttgattcctc
     8761 cagtctaatt ttctcagtaa tcaatgcagc aatctcagat ttactttgag cttcttccac
     8821 tgttccatct ttagataaga gatagccgtg gtgtgtctcg ccatggattt ccgtcaaatc
     8881 atttgcgaaa ataaaatcag ttttgttatt tatcagacta ttttttgcga ctgccagaag
     8941 agattccttt ggtacatcta ctaatagttt gaaaccaatg atttttgctt ttggctgcca
     9001 ttcacgtaga taagaaatga tttttggtgt tttcttcaaa acgagcacaa gatgctcggt
     9061 gttagaagag atctttgaaa cagtggtatc tgtctgttca ttttccgcaa accaattgtc
     9121 cagttcttct tgcgttgaag aagtgggtag gttttttgcc agctgttctt ctgaaaaact
     9181 aaaagcagga gtaaaatcac tgactgccat actgtggata atcgcatcgt agtgttcttt
     9241 tttcgttagt tcttctaatt gtaaaaataa gtccagtgca gattcgatcg tataaatcgt
     9301 tatattggag tgtcttttcg gtttcagtgc ttgtttcgta gtcacgtaat cgattgtggt
     9361 atccggattg gcagctaaat aatcagctaa tgcttgccct aatcgtcctg tcgaatgatt
     9421 tgtaatagca cgtacttgat cgattttttc agtcgttcct ccagctgtga ttagaatctt
     9481 catttatggt cacctacatt tactagtgtt cttccttggt gtgttccagc aagtagttta
     9541 gatgctgttt caggcaattg atccagagta atctcttgga cgaccagttc atctgcaatc
     9601 tgcaaatccg ccatctgttg ccataaagaa agacgttgat ctattggtac attgacagaa
     9661 tcaattccaa tcaactggat gtttcgcaga ataaaaggca agacagtcgc gtttagtttg
     9721 attcccccag cattgccaca taaagctact gcaccgttat aagagatcaa tggaagaaga
     9781 ctgcttagct gctcgccacc tactgtgtcg atgacatagt cgatttgctg ttttcctaaa
     9841 gcttttgttg tctctggcag aaattcatca ggtcgtacga cttgcaaagc acctagactt
     9901 tttagccatg tgacagactc ttcttttctg gaaaaggcaa tgattgactg gaatcctagt
     9961 ttatgtaaca gggctatcgc tgtactgcct acaccgccag aagcaccagt aacgacaact
    10021 tttttatcgg aggtcatacc ttgacggatt aaagcgttca cacacaacat agcagtaaac
    10081 cctgctgtac caaggatcat cgcttgacgc agcgtcatat tttttggtag aggaactaac
    10141 cagtctcctg gtactttttg atattggcta taaccgcctg gatgggtgac gccaagccca
    10201 aaaccagtag caagtacaag atcttctttt gaaaaacgtg gattgttact ttggacgaca
    10261 gttccggata aatcaatccc tggagtcatc ggatatgttc gtatcacctg accagactcg
    10321 ctcattgcca atgcatcttt ataattgaca tcagaatagg caatttttac caagacttca
    10381 ttttcagcca actcagtaaa tggcagatca ctgattgctg tatggaaaac aggttccttt
    10441 attacctgaa aagatcgaaa agcgttcatt cgtgagacac tccttttaaa attttctttt
    10501 atcataccat aaagcctact cttttattct tgttcttccg taaatttaga caaaaattag
    10561 gtgaaaaata aagaatcaaa caactagaat ttattccaaa agataaagaa atattcagca
    10621 aaaaaaattt tacaaagggt tgcctttttc aataaatttc gtatactaat acgagtggta
    10681 gtaaacagga agtttactat tagaaaactt cgattcggag gaaattatgg aaattggtga
    10741 gaagttaaga aatttgcgca tccaaaagaa tttgacacaa gaagaactag gcgaacgcac
    10801 agatttgtca aaagggtata tttcccagtt ggaacgagat ttaagttcac catcaatgga
    10861 aacattcttt tctattcttg aagtcttagg tgtgactcca gaagaatttt ttcatcaaga
    10921 agcagcgaat ctgcaagtgg tttattcaaa agaagatcat acggtttatt tcgatgaaga
    10981 aaacgggtat gaattggaat ggctcgtggc tgattcgaat gaaaaagaaa tggagcctgt
    11041 cttgctgact tttgaaggtg caggagaata taagacgttt gaaccttcat tatctgaaac
    11101 gtttattttt gtattagaag gggaattaga gttaagctta ggagagacgg tctatgtggc
    11161 aaaagcagga cagtcgatct attatcaagc gacaaaacaa catcaattaa ggaatcatct
    11221 caaaaaacga acaaaagtat tgattgttgc aacgcaatca tatttgtaat atagggggta
    11281 aaaacgtggg gaaaccaatc atttcatttg atcaagtagt gaaacgctat gatgatgaaa
    11341 cagtattaaa aaaagtaagt tttgagatcg aacaaggaaa attttataca ctgcttggtc
    11401 cttccggctg tggtaaaaca acgatcctac gtattatagc tggattcacc gaagctacag
    11461 aaggggacat ctattttgaa gggaaacggt tgaatgattt gccagctaat aagcgacaag
    11521 taaatacagt cttccaagat tatgcacttt ttcctcatat gaatgttttt gataacgttg
    11581 cttttggatt gaagatcaag aaaatgtcca aatcagatat cgaaaagaag gtaaaagatg
    11641 ctttgcgtat ggtccagctt cctggatacg aaaaacggga gattagtgaa atgtctggtg
    11701 gtcaaagaca gcgtgtagcg attgcacgag caattgtaaa tgaaccgaaa gtattgctat
    11761 tagatgaacc gttatcggcg cttgatttga aactacgtac agatatgcag tatgaactgc
    11821 gggatctgca acaacgatta ggaatcacgt ttatttttgt tactcatgac caagaagaag
    11881 cgttggcaat gagtgatgag atttttgtca tgaataaagg gaaaatcgtc caaagtggta
    11941 cacccgtaga tatttatgat gagccaatca atcatttcgt tgctgatttt gttggggaga
    12001 gcaatatcgt ggacggtgta atgatcgaag ataatctagt tgagtttgtt ggtaagcaat
    12061 ttgaatgtgt cgatggcggg atgcgtctaa atgaaccagt tgaagttgtt ttacgcccag
    12121 aggatttgac gatcacgaca ccagataaag gaaaactagt cgtaacggtc gatactcagc
    12181 tgtttcgtgg tgtccattat gaaatcatct gttatgatga acagcaaaat gaatggatgg
    12241 tccattcaac gaaaaaagct aaagaaggaa gcaaagtagg attggccttt gagccagaag
    12301 atatccatgt catgcgtttc aacgagtctg aagaagaatt cgatgcacgt cttgacagct
    12361 acgaagaata ggggggacaa gaatgaaaac aatgcgcagg atctattccg tcccttatat
    12421 tttgtggctc ggtttattcg tgatcgcgcc tgtattaatg atcatttatc aatctttttt
    12481 tgatatgaat gggcaattca cgctgaataa ttatctatct tattttacgt cggggacatc
    12541 gctttctatg acgatcaatt ctgtatggta tgcatttttg atcacgtttt tcacgctgat
    12601 catcagttac ccgactgctt attttttaac aaaactgaag cacaagcaat tatggctgat
    12661 gctgattatt ttgcctacat gggtgaatct gctgcttaaa gcttacgcat ttattgggat
    12721 tttcagtgtg cacggcagcg tgaatcattt ctttgatttt atcgggatcg ggccgaaaca
    12781 gatattattc acagacttca gttttctgtt cgtggcaacg tatatcgaga ttccatttat
    12841 gattatgcca attttcaatg cattggaaga gatgaatcct tctttgatca gtgctagtag
    12901 agatttaggc gcgaatagct ttgagacctt ccgacgagtg atttttccgt tatcattaaa
    12961 tggtgtgaaa agtggtgtcc aagcagtatt tattccttct ctttctttat tcatgctgac
    13021 tcgtctgatt ggcggaaatc gggtgatcac tttaggaacg gctatcgaac agcatttctt
    13081 ggttacacaa aactggggga tgggttctac aatcggtgtg attttaatca ttgctatgtt
    13141 catcgtgatg ctcctgacag gcgagaagaa aaaaggaggt cgctcataaa tgaaaaaatt
    13201 taaatggtcg tatctttatt tagggatcgt ctttttattc ttatatgcac cgatcttcta
    13261 tctgattttt tattccttca atgaaggagg aacgatgaac agctttacag gatttacttg
    13321 ggaaaattat gaagctgttt ttgaagatac acgtttgatc attatcgtat tgaatacgtt
    13381 catgctggct ttcttatctg cattgatcgc cacgatcatc gggacatttg gggctatggg
    13441 aatctattac acgaaaaaac gacgtgcacg gacagcgttg ttgagcatga ataacatctt
    13501 attagtctct cctgatgtta ttatcggtgc tagtttcttg attttcttta cactactagg
    13561 attcggatta ggttttcaga gtgtattgct ttcccatatt gctttcagca ttccaatcgt
    13621 cgtcttgatg gtcttgccaa aattgcagga aatgaacgat tcaatggtcg atgcggctag
    13681 agaccttggt gctaataatt tccaagtagt caaaaatatc attttgcctt atctttctcc
    13741 aggaatcata gctggatatt ttatggcatt tacctattct cttgatgatt ttgcggttac
    13801 atttttcgtc acaggaaatg gctttacgac gctctcagta gagatctatt ctcgtgcaag
    13861 acaaggaatc agtttagaaa tcaatgcgct aagtgcgtta gttttcttgt tctccatgat
    13921 attggtcatt ggatactact ttattagtaa agaaaaccgt ccaaaacggc tgaagaaaaa
    13981 cagaagagta cgcgaggggg tggcacggct gcgatgaaaa aattacaatc tctgttttta
    14041 ggtattctca tcattattgc ggtgctgctg cttggtgttc gtcaacttga aaaaacaagt
    14101 ggcatgagtg gagcaaaggt acttaccatc tataactggg gagattatat cgatcctagt
    14161 ttgatcacga aatttgaaaa agaatatgga tacaaagtga attatgaaac atttgattct
    14221 aatgaagcga tgttcacaaa aatccagcaa ggtgggacaa actacgacat cgctatacca
    14281 agtgaatata tgatccaaaa aatgatcaaa gaaaaactag tacttccatt agatcattcg
    14341 aagatcaaag gattaaataa tattgatcca cgatttttag acttggattt tgatccagat
    14401 aatcagtatt cgattccata tttttgggga actttaggga tcgtatataa cgataaattc
    14461 tttgatgcaa atcaaattaa gcattgggat gatttgtgga agccggaatt aaaagacagt
    14521 ttgatgctga ttgacggtgc acgagaagtt atggggcttt ctttgaatag tttaggctat
    14581 tctttgaata gtaagaatat ggcacagctg acagaagcag caaataaatt gaatcattta
    14641 acaccaaatg tcaaagcaat cgtggcagat gagatcaaaa tgtatatgat caatgaagaa
    14701 gcctccgtgg cagtgacatt ttctggcgaa gctgccgata tgatgtggga aaatgaacat
    14761 ttacattatg tgatcccacc agaaggatcc aatctttggt ttgataatat cgttatgcca
    14821 aaaaccgtga aaaataaaga aggtgcctat gatttcatca actttatgct agaaccggaa
    14881 aatgcggcac aaaatgcaga gtatattggt tattctacac caaatgaaga agcaaaagcg
    14941 ttattgccaa aagacatttc gtcggacgaa caattctatc catctgatga cacgatctcc
    15001 catcttgaag tctacaaaga tctaggatcg aaatacctag gcatctataa tgacctattc
    15061 ttagaattca agatgtatag gagataatgg caaaactcag taagaatggg gacttatcaa
    15121 cgttctaaag agctaaaaat cgtccttttt ctatctgagt tttccaatct tctaccaaaa
    15181 gcggatgaaa cgccgctttt tttgtgttat tttatttttt ctaccacgtt tttctaccag
    15241 agaaaagtgg acaaaaccat tgtaaaatag ggcccgagac gggctagtaa atctaagatt
    15301 gaaaaagtta cagaaggata aagtaattgg aaactatgca aatatactta acccttctgt
    15361 aatctattaa taaaactaat atatgaatcc agttcaatat ttgtagctat gaaaaaacat
    15421 aaaaatcata ttagataaaa actaaattgg tagataaatt attttgtcag atatctgagg
    15481 caaatattcg actatttgtg tttcaaagta aaaattggaa tacaaatata gaatagacat
    15541 attgcagccg atagtttatt atgcttttag acacattaaa ctggaggaaa aaatgaaaaa
    15601 gagcgctaaa gatcgagttt ttgatgagct gatacgacta acagggaata tgtcttctgt
    15661 cactgccgag aaaatttcga agaatctagg tatctctcgt cagaatgcca gccattattt
    15721 gacgagatta gtggaagata aaaaggtcga gaaattaccg ggaaagcctg ttttgtggaa
    15781 gcctttagat gaatatgcgg ttgtagataa tacggagcaa ataaacgaag cctttcattc
    15841 tgtagttggt catgacggaa gtctgagaga ggtcattcaa aaatgtatag ccgctgtcaa
    15901 atatccgcct aacggactca gtgtattaat aaatggagcc acgggtgttg gaaaaagttt
    15961 tttagccaca aaaatttttg aatacgctat ccatgagcaa attattgaaa aagatgcacc
    16021 atttgctatc ttaaactgtg ccgattatgc ggacaatccc gaactgcttt cggcaacact
    16081 gtttggatac aagaaggggg cctttacggg ggctgaaaag gataccgatg gtttattggc
    16141 aacggccaac aacggctatc tttttctcga tgaggtccat cgcctatcga aagaaaatca
    16201 agaaaagctc tttctattta ttgatactgg aaactatcgt ccagtcggtg aaaatgtaaa
    16261 ttggcattct gctaaggttc gttttatttt cgccacgacg gaaaaaggtg aaaattattt
    16321 attagacacc tttgatcggc gtattcaaat tagtgttatg ttgccaactt ttgaagatcg
    16381 ccccattcga gaacgattag agctgattca attatttttt caaaatgaag cggatgtttt
    16441 acaaaaagat attattgttt caagagaagc cttgtcttta atgttatctc atccctttag
    16501 tgggaatatt ggtaagctga agaatatcat taaaatcagt tgtgcggatg tgtatagtcg
    16561 aacacaagaa gaaccattaa cgattgggaa aaatgagatc ttgattcagt taaatatgct
    16621 ggaatccgaa gtagaaagct tgccgattgc tagtttattg ataaagaaaa atcaaaagat
    16681 aaaaacggat cgaatcaaac cgtttgatta tgattcgatt ttggaaaaaa ttactcagct
    16741 gataaatgaa ggtaatttca atgaaaattt ttcttcgata aagaatgaag tacagcattt
    16801 ggtgcatcac attgataagg attctttttt atcaatgagt agaaaattga tgattcagat
    16861 gtttagaaag gtctgggtat ccgttttagg aaaaacgtac ggattgacga cgagtggacc
    16921 tgttgcagaa attattagca gatcctatgt catttcccca agctataaga cagacttaac
    16981 cgggataaat cgtacattag ctgcaaatct acctcggacg acttacttat gtaatcagtt
    17041 cttggagcat ttaccaccgc tctcagaaaa ggacgagaac tttctaagac tgcttattgt
    17101 cgtgacttta tctgattatg tagatgaatc catcgagctg aagggcctgt tgcttgctca
    17161 cggagatagt acggctagca gcattcaagc agtagtgaat caattatgcg gcaattatgt
    17221 ttttgaagcg ttggatatgc cagtagagag taatgtgtca gagattgtcg aatcgacaag
    17281 gaatttcgtg aaacggcaag tgaatacaga tagtctagtg ctgatcgtag atatgggctc
    17341 tttaaatcaa atttacacac agatcaagaa cgatttaaaa ggggagttac ttcttctgaa
    17401 taatttgacg acttccattg cacttgatat tggcttgaag atgacgatga atgcgccatt
    17461 taaacagatt gcggaggatg cagcagacaa gtattcaatc aatgttcaat actttgaagg
    17521 cttttctcaa agtagcaaca ttatcatttc ctgtatgtcg ggtttgggga tttcggacaa
    17581 attgaaagat gtttttcgtc atcatttatc ggatgaatcc atcgaagttt tcacgaagga
    17641 ctacagagaa ctacgtgaat taatcgacca aaatgatgaa acttattttg acaagacaaa
    17701 attggtcatc acgacttcgg atcttccacg ttccttttca attcctaata ttaatgttta
    17761 tgacatctta gacggtgatg gagcagccaa tttggagcat attttgagtc ctgaaatgaa
    17821 gccggcagaa tttaaccgct tgattcagga gctagtaaaa ttcttttcgt tggaaggaat
    17881 tgcggaccgt ttgaactttc tgaacccaac gatcgtgatg agtgaagtgg aaacagtcat
    17941 ttctaaatat gaaaattact atcatttcac tttaaatggc cgtgtaaaac taaatctcta
    18001 tatgcatacg gcattgatga tggaacgttt attcttggct cggacagaaa aatctgaaga
    18061 accgaattat gatttgaaac cagatgaaga tgaattttat tatgt
//
LOCUS       DAFFSV010000051        17267 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFFSV010000051 DAFFSV010000000
VERSION     DAFFSV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.051"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            541..1791
                     /locus_tag="LR110_001556"
     CDS             541..1791
                     /locus_tag="LR110_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBL4822520.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            1810..2820
                     /locus_tag="LR110_001557"
     CDS             1810..2820
                     /locus_tag="LR110_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBL4822521.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDVHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFEDGGTRVIHYTPETYIEKQ
                     NG"
     gene            2830..3483
                     /locus_tag="LR110_001558"
     CDS             2830..3483
                     /locus_tag="LR110_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL4822522.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDSVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3502..4500
                     /locus_tag="LR110_001559"
     CDS             3502..4500
                     /locus_tag="LR110_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBL4822523.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWKPTVSLEEGLRESYNWIEKQIKG"
     gene            4576..5337
                     /locus_tag="LR110_001560"
     CDS             4576..5337
                     /locus_tag="LR110_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HBL4822524.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5355..6461
                     /locus_tag="LR110_001561"
     CDS             5355..6461
                     /locus_tag="LR110_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL4822525.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRDVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6451..6684
                     /locus_tag="LR110_001562"
     CDS             6451..6684
                     /locus_tag="LR110_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822526.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            6883..7338
                     /locus_tag="LR110_001563"
     CDS             6883..7338
                     /locus_tag="LR110_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HBL4822527.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7352..8125
                     /locus_tag="LR110_001564"
     CDS             7352..8125
                     /locus_tag="LR110_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL4822528.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8112..9242
                     /locus_tag="LR110_001565"
     CDS             8112..9242
                     /locus_tag="LR110_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL4822529.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9273..10508
                     /locus_tag="LR110_001566"
     CDS             9273..10508
                     /locus_tag="LR110_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822530.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFVPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLSVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10533..11642
                     /locus_tag="LR110_001567"
     CDS             10533..11642
                     /locus_tag="LR110_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822531.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAKYEKYMDFVWEE"
     gene            11684..13018
                     /gene="gmhB"
                     /locus_tag="LR110_001568"
     CDS             11684..13018
                     /gene="gmhB"
                     /locus_tag="LR110_001568"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBL4822532.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            13053..14690
                     /locus_tag="LR110_001569"
     CDS             13053..14690
                     /locus_tag="LR110_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HBL4822533.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            14792..15187
                     /locus_tag="LR110_001570"
     CDS             14792..15187
                     /locus_tag="LR110_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HBL4822534.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            15205..15936
                     /locus_tag="LR110_001571"
     CDS             15205..15936
                     /locus_tag="LR110_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL4822535.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            15960..>17267
                     /locus_tag="LR110_001572"
     CDS             15960..>17267
                     /locus_tag="LR110_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBL4822536.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISA"
BASE COUNT         5531 a         2445 c         3825 g         5466 t
ORIGIN      
        1 tatcgtagga tgtgatgatg aaagccgtga aattgttcaa gtatttacca atgtctccct
       61 gccagtaaat ccgcttagaa tttgagcctt gaaaatgtgc aatgataaaa aacaaaaact
      121 cgctcaaaat agacagtttg gggtgggaat agaccaataa tgtaccagac aaacttccat
      181 ttggggtgaa aataggtcaa taaagcgatg ttaaacatac ctttggagta agaatagagg
      241 gtaataagtg aatttataaa ctttttggtg actggttgag tggaaaatgt aggtacattt
      301 caatgcgaaa atgtattata accccaaacg aggtcaataa aaatgactca aacttgcaat
      361 ataaccctaa acgggtaggg gatttatgaa aaattgaatt tgccggcgta ggagctggct
      421 gttttaagta tgaatgaaca cagctttcgg gctgttttat ataaatagca ggttactgtt
      481 cacaaacagc attttgttaa gaaagtagtg accacagcag tggtggaaag aggaaatatt
      541 atgaagaaac agtataacat tgcagtcgcc ggcaccggtt atgttggttt gagtattgcc
      601 actctgttgg cacagcacca taaggttaca gcggtggata tcattccaga gaaggttgaa
      661 ctgattaata accgtaaatc tccaatccag gatgattata ttgaaaagta cctggctgag
      721 aaagaactga atctgaccgc aacattggat gcaaaggcag cataccagtc tgcggatttt
      781 gttgttattg cggctcctac taactacaat agccgcactc aacattttga cacatccgct
      841 gttgaggctg ttattaggct ggtcatggaa gtaaatcctg atgccattat ggttattaag
      901 tccaccattc ctgttggata caccgcttct gttcgtgtaa agactggtag caagaatatt
      961 attttcagcc ctgagttcct gcgtgaaagt aaggcactgt atgacaatct gtatccttcg
     1021 cgtattattg tgggtaccga tatggatgat gagcgtttgg ttgaggatgc acacactttt
     1081 gctacactgc tacaggaagg cgcaatcaaa gagaatgtgg acacattgtt catgggcttt
     1141 actgaagcag aagctgttaa gctgtttgcc aatacatatc tggctcttcg tgtcagctac
     1201 ttcaatgagc tggacactta tgcagagatg aaaggcttga acactcagca gatcattaat
     1261 ggagtttgcc ttgatcctcg tattggtact cattacaaca acccttcctt tggttacggt
     1321 ggctattgcc tgccgaagga taccaagcag cttctggcca actacgcaga tgttcctgaa
     1381 aatctgatcg aggcaatcgt tgaatctaac cgtactcgta aggactttat tgcggatcgt
     1441 gtacttcaga tggctggtta ttacaattac gcagaagcaa atcagtatga tgctggcaaa
     1501 gaaaagcctg ttgtgattgg tgtttaccgt ttgaccatga aatccaattc tgataacttc
     1561 cgccagagtt ctatccaggg cgttatgaag cgtgtaaaag ctaaaggtgc aactgttgtg
     1621 atttatgagc cgaccttgaa agatggagag actttctttg gcagtaaggt tgtgaatgat
     1681 ttggaacagt ttaaggcaat gtgtgattct gttatagcaa atcgctacga tgcttgcctt
     1741 gatgacatta aggaaaaagt atatacccgt gatatcttcc agagagacta agattaggag
     1801 agagtattta tgattattac aaaaacaccg tttcgtatgt ctttttttgg cggtggaacg
     1861 gatatggagg attatttcaa agagaatggc ggtgcagttc tttctactac ttttgataag
     1921 tactgttatg tgaatgtgag acatctgcct agattttttg attacagcgc tgagttatct
     1981 tattctcgta ctgagagagt aactagcatt gaagatattg agcatcctgc gattcgtaat
     2041 gcgatgaaaa tgctcgatgt gcatgaaatt cggcttacat acgaagctga tttacctgct
     2101 cgctctggtc tgggtacatc cagttctttt gcagttggta tgttgaatgc tttttatgca
     2161 ctggagggta agtatgctga caagaagaaa cttgctgatg aagccattta tcttgaaaga
     2221 gttctgtgta atgaagccgg tggatggcag gatcagattg ccgcatctta tggtgggttc
     2281 aatcgaatta atttcaatgt ggatggttat gaggtgctgc ctgtcattat ttcgcctgaa
     2341 cgtaaaaagc agcttaacaa caatctgatg atgttcttta ccggattcac tagattttct
     2401 tctgatgttc aaaaggctaa caacgtcagt ggcacagagg ataagagagt aagactgaaa
     2461 aagatgtatg agcttgtaga tgaagctgag gcaattctta ctgataagaa cagaaattta
     2521 gatgatttcg gtagacagct tgatgttact tggagattga aaaaaggtac tggtggagca
     2581 attagtacag gaagcattga tgaactatat gaaaagggta tggctgcagg tgctttaggc
     2641 ggaaagttgc tcggtgcagg tggtggaggt ttcttggttt tctatgtaca acctgagaaa
     2701 caggatgcag ttcgttgggc tatgcgtgat ttgatgtata ttccgtttga atttgaagat
     2761 ggtggaacta gagttattca ctacacgccg gaaacttata ttgaaaaaca gaatggataa
     2821 ggagtaacaa tgttagcgga aaaacttaat aaacatattg acttattgat taaacgatat
     2881 cctacacttg agacgattaa acaggacatt atcgatggat acctaattat ggaggaatgc
     2941 tatttgaatg gtggtaaact tcttgtggca ggaaacggag gatcagccgc tgactcggaa
     3001 catattgctg gcgagttgat gaagcggttt aagacgccac gtcctgtaag taaggaattt
     3061 gccgataagt tagttgcaat tgactctgta agaggtccgg aattagccaa aaatctcgag
     3121 tgtggtttaa cggcaattcc tttggtcgca cacgaagcat tgtctactgc ttatattaat
     3181 gatgtcgatg gtttgggtgt cttcgcacag cagctgtttg gctttggtag atctggtgac
     3241 gtattccttg gtatttcgac gtccggaaat agcaagaata ttatgagcgc aactgttgtt
     3301 gctagggctc tcggtataaa agttattggc ttgacgggcg caaagggtgg ggaacttgcc
     3361 acggtagcag atgtttgtgt taaggctcct gaagttgaaa catacatgat tcaagagtat
     3421 cacttgccga tatatcattg ttggtgtttg atgctggaag ataaattctt tggaaacgaa
     3481 taaaatttgg aggaatgtta aatgaaaaag acaattatgg ttaccggtgg ttgcggtatg
     3541 attggctcca atcttgttaa gagactggtg aaggaaggct gctgggatgt ttatgtagct
     3601 gataatctgt ggagaggaaa gttggagtac ctgaacgacg aagatgggca tcctgtcatc
     3661 gacctggata ctcatttctt caatgctgat ctgactgatt atgagcaggc taaaaaggtt
     3721 attggtacga ctgagtatgt agttcatctg gctgatgttg tggctggcat tgactatgtg
     3781 tttaagaacc agggcgaatt gttccgaatc aataatctga tcaattctaa tgtttttgat
     3841 tgttgccgta aggctggcaa ggagaaaatc aagggtgtta tctatgttgg taccgtatgc
     3901 tccttccctc tgaccagaca gaatactttg aatcctgagc ctctccggga agaggagttg
     3961 ttccctgctc ttcctgagtc tgcttatggc tggagcaaac tgatgggcca gttggagatg
     4021 gggtatttgg aaaaagaaac cggaattccg tgctgtatcc tgatgcttca taatgtttat
     4081 ggcacaccta ctgattttgg cgaacgtagt caggttattc ctgctctgat tcgtaaagca
     4141 atcaataatc ctgaagagcc ttttaatgtg tggggtagcg gagagcaggg acgtgctttc
     4201 attcatgtca atgatatcgt aaatgcattg gtgctcgccc tggataaggg ttggggtcat
     4261 ggtcatattc agattggccc ttctcactgt acttccatca aggaaattgc atataagatc
     4321 attgaaattt ctggcaaaga catcaagcct ttctttgata cttctaagcc tgaaggtgat
     4381 aaggctcgtt gcgcagatta ttctaaggcg aaagagattt tggattggaa gcctactgtt
     4441 tctttggaag agggtcttcg tgaatcctac aattggattg aaaagcagat taagggttaa
     4501 taagtatata agctgttctt cctccgtgta attagaggat gcggaggaag aacatatgag
     4561 taaggatgta ctgagatgga aaagaatatg tataaaagaa aacgtgattt cactgtgtgt
     4621 ggaatagtag gtgtttgtgc tactacaaag aacgagtttg taagggaagt tttttccatg
     4681 ccaacatctc cggtaagtgc gactgttgat ctcgtgggag tgccggccat tatttcggca
     4741 aaggaaaatc ccaaaattgc tgagatgtat agccaggcta caatggctgc aatagatggt
     4801 atgcctattg ttaaaattgg acggcgcaaa ggatttacct gtgaacgatg tgctgcacca
     4861 gatattatgg gaatggtttt tgaagaaggc gttagacagg gaaaaacaca ttatttctat
     4921 ggtggaaaag ataacgaagt gttaaataag cttcgtgcaa acctagaaag agattacccg
     4981 ggaatcaaaa ttgttggaat gtattcccca ccatttcgtc cgttaaatga agaagaggat
     5041 agcaagattt gcgaggaaat caacaactta aatccagatt ttctgtgggt tggtattggt
     5101 gcgcctaagc aagaaatgtg gatgtgggaa caccagaaga agatccataa cacagtaatg
     5161 ttcggtgtag gcgccggctt taactttttt gcgggcactt tggaaaaagc tcctgaatgg
     5221 atggaaaaag ctagtctaga gtggttattt agactggtaa aggaacctcg tagactatgg
     5281 aggcggtata tcttgggagg tataaagtat atctactata ctattgaatc taagtagggg
     5341 ggcaataaaa atagatgaaa atacttatag tagttacaat gacgaataac tttggtaaga
     5401 aggggtccta taattctcag gaaataggat tagctcgagg gttagctgaa ttaggccacg
     5461 aagttattgt atataaaagt gtaaaaagtg atgaggaagt tggaacagag caattaagca
     5521 atacggcgat gattcactat atcaagtgtc gttatgtagg atcaaatgga ttcttatcaa
     5581 caaagaattt tgataagaat gcggatgttc taatacaatt tgcggacctt caactatcag
     5641 tacctcatgt atactcatgg actgtaaaaa atggaataaa atatatacct tataatggga
     5701 taacgcgaag tcaaagtggc aataaatata taaagacaat tattgaaata ttggcgcatc
     5761 gaaattataa aatttataag aaaaatgggt gtgttgccaa aaatgatcga gtttctgaaa
     5821 aattagtaca attaggagta aataaagtga aacttgctcc tgtttgtgtg gattttgaac
     5881 agttacataa gaattacgaa gcaatagatt ttactactct cagagaaaaa tggaatattc
     5941 ctcgcgatgt tcgtgttatt ttatatgtag gacggtttga aaaggataaa agaccgttag
     6001 atatgattag tttatttaat aaacttgatg agcataatta tttgctgatg gtaggaactg
     6061 gtgctttact cgagcaaacg aagcagcaga taaatttact tggactgcag aatagagtaa
     6121 aaataattga gcgtattcct aacaaagata tttgggagtt atattgctta tcggatgtat
     6181 ttgtaaatct aaactatact gaaatttggg gtatggtttt gctagaggca atgtactatc
     6241 gcctcggaat tattgctgtt aatgcacctg gcccacagaa tattattttg gataatagat
     6301 atggaatgtt ggctaaatct gacgatgaaa ttttgacatt cttaaaagac ggacgattta
     6361 ccaacgatat catagatgca gcatatcagc gtgtaactac agaatttaga tggtcaaagt
     6421 cgggagaaat tatgcttagc atgttggaag gtgataaatg atatggaagt aacgaaatta
     6481 atggcattga ggaatcgata tgctttaaac atagttgata attgcacgag aaaaatagcc
     6541 aaaattcttg gttgttgtat tggtaaaggt gcacagatag ggaactctgt tgaatttgtt
     6601 cataattctg taggcactgt gattcattct gacacaatcc ttgaagatgg tgttaaagtt
     6661 tatcagaatg tgacgtgtgg ttgagcagat agtaatgaac gtatcatcat tcgagtaaaa
     6721 aagaacgctg ttttatgtgt gggtgctaaa attctttgta aatctaacac agaattatgc
     6781 gttggggaag gtgctgtagt tgctgcaaat acagtggtat tgaggtctat tccaccctac
     6841 gaagtgtggg gggtacctgc taaaaagatg gggagataaa acatgaatgc atataaatta
     6901 tacaaagtag aacgatggtt ttacttacac aaaatggagt tttttgccaa gattgtaaag
     6961 gcactaattt atgtacttca taatagctca atttcatatc aaacagaaat tggagataat
     7021 tgtaagttct tatacggtgg cataggttgt gtaattggaa aagatacaaa aataggtaat
     7081 cacgttataa tcggaacgaa tgtactgact ggtggtagat ccaataagcc tggtatgcct
     7141 acaattggaa acaatgtata tatcgctact ggcgcaaaaa ttttaggtaa tatcacaata
     7201 ggggacaatg taatagtagg ggctaatgct gtggtcatta atgatgttga acctaattgt
     7261 tcggtaggag gcgttccggc tagagtattg aaaaaagata ttgatgtaaa tgattattgt
     7321 agtttgaatc gttactaaaa ggagagattg tatgttgtat tttgttattc ctttaatgag
     7381 tgcgaaggca gcaaacaatt gggagatggt tagtacctta tttaaccgaa cattatggtc
     7441 gtgttataat cagactgatc ctgatttcaa aatcattgtt gcttgccatg aaattcctaa
     7501 actcacgcat gaatatgatg acagagttga atttattcaa gttagcgaaa aggatgcccc
     7561 tattcccaca aatcaagacg aaaaaatgat tgataagggt tataaaacac atgctctcgc
     7621 aatgcgtttg cgagagttgg gcggcggttt cgctatgatg gttgacgcag atgatcttgt
     7681 aagttctcat ttggcggaat atgtgaattc ccatccccag gaaaatggat tttatgtcaa
     7741 aacgggatac gtgtactttg ttggagacga ttatatgaag gttctaccaa agttctcatc
     7801 aggaagtgcg tgtatagtaa aatatgcagt ggaagacctg cctgaggcat atccggaagt
     7861 tatgacagca aattgtgatg ataatccatg gatcattaga aaacgacatg ggggcgttgt
     7921 tccagcatgt gaagagcaag ggcgtccatt aaaacctctt ccattcaaag cagcagtata
     7981 tgtattagga acaggggaca atcactcgct ttatggtaaa acgacaaaat atcagacgaa
     8041 gatgcgagag atactggaaa tgtttatagc aaaacgtaaa attaaaggaa aactgaaagc
     8101 agaattttct gttgattggt tataaggtga gtattatgaa ggtattgatg tgtgaaagcc
     8161 aaatgccaga gaattggtat tcgtttgatc tttctaaaga acttaatcaa tatgttgatt
     8221 taacattgct gtgtaagcgt gatgttcctc ctttagatcc taaaattaag agcaaacata
     8281 ttttgtatca taaaagtaca aataaagtgt tttcggcact tgtttttctg aaaagcttgt
     8341 tcttaatttg gaaggaaata agatttggtg attatgatat tgttcatttg caattgatga
     8401 aaagtctcaa atatgaaggc ggattgtacg ttttatgtaa gagacactat aaaaaattaa
     8461 ttatgacagt tcataatgtg ttaccacatg aggttcgtga aaaggatcgc caagagcact
     8521 ttaatatata ttcaaattgt gatggtttca ttgttcacaa tcgagtaaca gaacagcatt
     8581 tatatgctga attcccagaa ttaaatgcaa agtcatatgt ttgtccacat gctatctatg
     8641 gagatgttaa acatatatct agagcgtatg ataaagataa gtttgaattt atcttgtttg
     8701 gacaaattcg tccatataag ggaattgatg tactgcttaa tgcaatcaca gaacttccaa
     8761 tggatgtaag aaagaaagtt cattttacca tagcaggtca acaacatcct aaacaggata
     8821 gtactgacta taaaaaaatg attgaagatt taaaggtttc agattgtgtt ctatataagc
     8881 cagagagaat ttctgatgaa gaaatggaca gtatgttcag aaatgcagat gccgcaatat
     8941 ttccatataa agaaatctat ggaagtggag cgcttcttat ggcatacact tattgcttac
     9001 caactattgt tagtaatgta cctgctttta tggaagaaac cgaggaagga aagatagggt
     9061 tacttttcga aaatttgaat tctaagagcc tagcagacac aattgtaaag tatgtgaacc
     9121 ttagcccagc tgagaaaagc aaaatagagg atgatatata tgagcttgta aaccgcaagt
     9181 atacctgggc ggcatctgca aaactcacat acgaggcata caaacaaatt ttgaatgagt
     9241 agtaaaacaa ttagaaagga ataataatta acatgcagat tagtaaatgg aaaatcatac
     9301 agtttctaat ggtctttcta atgattatct catcaagcat attgaagcct ttgggcggct
     9361 ggttgggaga aacgatgaac gttagacttg tgacagtcat tctagcaata gtatgtttgt
     9421 taatagcctc attaccagca tttagaaaaa atagatattt aaatttctgc actttactga
     9481 tttgtgttgt tataatagca gcttacatct actcagataa gacaatatgg gttaaatcta
     9541 gggaaagtat gagtttatac tatgtattat tggcgtatcc attgttctgt actttaatta
     9601 accaagcgtg gaatttggat tcaatgctaa atacgatatg tggtgtaaca tttctttcat
     9661 acattcttag aacatctatt tcacttattc agaaattttc aggagtactt ctctatgaaa
     9721 acatatatcg tgagtgtgcc ccggaaaatt ggtatcgtgg aggcttttta agaattaatc
     9781 caccatgttt tacaattata ataataccga ttgctttgta tatgattgag cgacaagttg
     9841 tagctagaag aaaagtaaaa tattatttac tgattgctag cgcactggca tattctgctg
     9901 ttattcatgg ttctagatct gtccttgtct atcaaattat tgtacttggt tgctatatcc
     9961 tttttaagaa aggttcttcg aaaagaaaaa ttgcaatgtg ggttttagct gggttgcttg
    10021 tagtcatatt tattaattcg accatgttct caacatttgt tgaaactttc acaaacagta
    10081 caggtgaata tgcaggatct gctgaatctc gatttgcttc agtttggttt tttgttccta
    10141 aatttttgca atcgcctttg ttcggtactg gtattcttac tggtgaggaa tttacgttta
    10201 tattaccagg aggagttaga ggacacctat ctgatattgg atttttctat acaattactc
    10261 aatatggtgt aggtatactt atatttgata ttctgtttat tataaaaggg ttcaaaacag
    10321 cattacttgc atcaaaagtg ggtatgactg gctatcaata tttagcattt ggaattacat
    10381 taagtgtttt gctaacaggg attaatatag actggtttta tccgatattt actccagcta
    10441 ttcctgtatg cttagcggtt atagaataca tctatcaaga atgtagaagt gtggctaaca
    10501 tagaatagga aatattaata ggagtgcatc tcatgaataa gaaagagatt ttatataaca
    10561 tattgaagaa gtacgaaaat ttaccatatg tgtttaggaa catgtttggt cataatataa
    10621 gtaaactcgt atcttggaaa ttgactgctg attgtgcaac agtagaagaa aattctgaag
    10681 tttatgcagc gtttcaattg cgctcaaagg acatgtctga tgttccagtg atgggtttag
    10741 ttaattgttg caaagatgta gcaattgtaa tgcaggggcc actatgttta aaggatgatt
    10801 tctcttataa tactctggcc tactataaga aatgttatcc aaatgcttta attattcttt
    10861 cgacatggaa ggacgaagat attaaggcat tgaaaaggat agaggatctt ggagttatag
    10921 ttatttgtag tgagaaacct gaaaagcctg gacacttaaa tattaattat caagttggaa
    10981 acactttgcc tggaatagtc agagcaaagc agcttggtgc aaagtatgtt tgtaaaacta
    11041 gaacagatca aagaatatac catccaaatg caatggcgtt tttctgcagt ttacttgaac
    11101 agtatcctgt taataacgaa gatgattgga aattaataca aaatcagcga ttactgttac
    11161 tatcaatgcc gtatggagac atgtttttcc cgtattgttt gtcagacttc ctgtattttg
    11221 gtgatgttga tgatatgata aacctcttct cgattcctag tgatgtgaga gaaaagggag
    11281 ctgtgtctag aggagtctca agaagggaga tatctgaaaa taatttagct ccagaagttc
    11341 agcttcttcg aagctatatt tcacgaatgg ggggcaatga ggagtgctct attagggcat
    11401 attgggagtt tgtaaaaaac catgttatta ctattaacaa aaatcaaatt gatttgtact
    11461 ggcataaata tgtaggtcgt tattcaaata atactattta tggtacgtat tatattgatg
    11521 atagccaaga tgcacttcac tgctataatt ttgattttat aaattggttg aatttgtaca
    11581 ctggaaaata cgaatacaaa gcaaaatatg aaaaatatat ggactttgtt tgggaagagt
    11641 aattaacttt tatagtaaaa agtactttag ggaagggcat attatgaaaa cagtaattat
    11701 ggcgggcgga cgaggaagcc gaatttcgga attgtttccg gatattccta agccaatgat
    11761 tcctatagat ggcattccag ttttagaacg agaattgtgt tcgcttagag atcaaggctt
    11821 taatgatatt attttgactg tttcctattt gcatgaaaaa atagaggagc atttcggaga
    11881 tggttcaaaa ttaggaatta aaatcaaata ttttattgaa aaaactccac ttggcaatgc
    11941 aggtgcattg tattttttgt tggatgaact gggtgatgaa ccgtttttac ttttgaatgc
    12001 tgatgcagta tttgatgttg atttcaggca gatgattaga tttcatgttg agaataacgc
    12061 agatgttact ttgtttacgc atcctaactc acatccgtat gatagtggat taattatatc
    12121 cgatgacaat aagaaagtgc ttcaatggtt agcgaaggaa gatcaaagac ctgaatatta
    12181 taaaaaccga gtaaatgcag gtcttcatgt tataaatccg aaggtgttga gagaaaatcc
    12241 attaatggaa attgtaaata aatcaatagt tgaaggtggg gggctaccat acaaaattga
    12301 tttagataga atgattctga aaccattggc taacacaggg cgtatgtatt gctacgattc
    12361 tccagagtat gttaaagata tgggaacacc ggaccgatat agagctgtaa ttaaggactt
    12421 tcaaaaggga attattgttg gtaaaaatct atccaatcca caaaaagcaa tctttttaga
    12481 tagagatgga acgattaata aatacgttgg ttttttgaga aatgcagaag aatttgagtt
    12541 aattgatgga gtatcggagg caattaagat tattaattct ctaggttttc tgtgtattgt
    12601 tataacaaat cagcctgtaa ttgcaagagg tgaagttact gttgatgagc ttaatagtat
    12661 tcacaataag cttgaaacaa tgctagggca tgctggtgga tttttaaacg gtatttatta
    12721 ctgtccgcac catccccata aaggatatgc aggagaggtt atagaattaa aaattgattg
    12781 caattgtcgc aagccaaaac ctggactgtt attagaagca tcaaaggatt ttaacataaa
    12841 cctaagtcaa tcatttatgg ttggagatag tgaaagtgat gtaattgctg gaaaaaatgc
    12901 tggttgtaag acagtactga ttacagaaga catcaccttg cctgctaatt atggccaaga
    12961 ctatagtgtc tgttcattgt tagaatttgt taaaaatcat ttatgcacta tgcaataatt
    13021 ggtttagtga aacttgtaaa aggagtaatt agatgaaggt attaattcca gcagcaaaag
    13081 ttgttgaaaa agaattgcag agtataggaa tgttaccagc aattatctat ccaattaata
    13141 aaaaaattgt ctttgattat ttgtataatc agtataatgc aatctgcgat tcattggata
    13201 ttatctgtta tgaaaaagcg gataaggttc atagaagact gaaaaactat catgattcca
    13261 gaattcatat atgtgacctt cctgaattag gggatctagg gcaaaccgtg tattttgggt
    13321 tatcagatga agatgagcct gttctaatta acttcgcaga tacaattgtt atggagagta
    13381 tgtctgatat agaaatggat acattctatt attctgaaga ttttgtgtcg agtacttgga
    13441 catactttaa aattaatgaa ggaataatta ctgatatcca cgataaagag gaagcttacg
    13501 tatccgataa acgtgaaaaa ttatttattg gcgtatttat gttttcttcg tcaaaacttc
    13561 tgaagagttg cttaagagag gcagttgaga gtcggaaaca ggggggaata aactcatttt
    13621 atcgggcttt acagctatac agcgaacgtt gtccgctaaa ggccgtaaga acagaaaagt
    13681 ggtttgatat tggacacatc gataaatatt acaatacaaa gttggaagtt aaagcacgag
    13741 agtttaatca tatctctata gataaagatc gagggatatt gaagaaaact agcgatgaca
    13801 aggataaatt tattggagaa ataaagtggt atttaaaact tcctgctgat attgaatatg
    13861 taagacctag aatttttgat tattctacat cttacgtagc tccctatgtt gatatggaat
    13921 attatgcata tcatacggtt catgagttat acttatatgg tgatttaaaa tataaccagt
    13981 ggcatgatct gtttagccgt ataaagttta tctgtagcga ttttaagcga tacaccttaa
    14041 aagatgaaaa gattagagag tcattggaag atatgtattt gcgaaaaacc atgcagagat
    14101 ttcaaaaatt gtctgaagat aaaagatttt cagctttttt tgagaaacaa attcaaatta
    14161 atggtcgtaa gtttatttct ttaaccggtg taattgaact tctaaagaat cttatcccaa
    14221 gtatgctcta tgatgtaaat gaattttgta ttatacatgg agatttgtgt tttgctaata
    14281 tgatggtaga ttccaattgt tcctttgtta aggttattga tcctagggga aaatttggaa
    14341 aatatgatat ttatggggac agcagatatg aattagcaaa actatttcat agtttagatg
    14401 gtaaatatga ttatatcatc aaggatctat ttactgtaga atatgatacc gagtgtgcag
    14461 tgatagatta tacaattaat acgcgtgtaa gagattacga tttatttgaa ttgtttaagt
    14521 cggtcttttc ggacgagatt aaggacgatt ttgataagat tgtattgata gaagcgttgc
    14581 tatttcttag tatgattcca ttacacggtg aaagttttga tcaccaaata gtgatgcttg
    14641 ctacaggtat ggaacttctt gcaagagtaa gtaataaagt tatactctaa tataaattgt
    14701 gccttaatga ataacggtta caatactgtt ttagcaatgt aggcattaag gcacgattat
    14761 attaattaat acaatagaag ggaagagaaa aatgtatagt gaatattctt ttgtgtttga
    14821 tatcgatggc actttgtgtc cgataaagaa gaaggatgag cgatatgagg atttggtgcc
    14881 gtatgagaat atggttgaaa aactgagatt ctataaggag aatggtgcta aaattacatt
    14941 gttcacctca cgcaatgtta attcctataa aggaaatttg ggaatgataa atgctaatac
    15001 tgcaaaagtg ctcttggcgt ggttggataa atgggacatt ccgtatgatg aaatcctgta
    15061 tggtaagcct tggccggggc ataaaggctt ctatgtagat gatcgaacgg ttagacctga
    15121 tgagtttttg agatgctctg tagatgaatt aaatgaaatc tgcaacagtt caaaaaaaag
    15181 attctaaata aggaggttag ttctatggaa aaaaagatga cagttatcat tacgatggca
    15241 ggtttgggaa gtcgttttcg aaaaattggt tatgcggttc ctaaatatat gattaatgcc
    15301 aaaggaagaa ctttatttga ttgggccatg gatagtttgg ttggatacaa taaaaatgtt
    15361 gataagtata tttttgttgt tcgtcaggaa gacaacgctt cagcttttat aaaggagcat
    15421 tgtgcttact ttggtattga aaacgtagaa gtgattgaaa tagactatat gacagatggt
    15481 caagcaacta catgtatgtt ggctattcct tattgcaatc aagacaatga gattatggtt
    15541 tacaatattg atacatacgt ggaatcaaat gagatgaaat acgaagatat ttgtggagat
    15601 ggacatattc catgctttca tgctgatggc gatcattgga gttttgcaaa gcttaacgat
    15661 cgaaatgaag taatagaagt ccgcgaaaaa gttcgcattt ctgataattg tactcttggt
    15721 gcgtattatt tctcatcggc aaagctgtat aaagctttat atgaagagta ttatgcagac
    15781 tcctctaata tggagaagaa tgaaaaatat atagcaccat tatataactt tatgataaaa
    15841 aaagggttaa aggtgactat tagcatagta gatgcgaaaa aggttcatgt gcttggtact
    15901 ccagaagagc tcaatgtgtt tttgaatcaa gaataaatag tgtttaggag aaaatagcag
    15961 tgaccagtaa taatctaatt gtaaaaacat taagcagata taagaatact ccacttcctg
    16021 ttaaggctgc attatggttt atggtatgtg gttttttaca gagaggggtt tcaatgatta
    16081 caaccccaat ttttactaga gttttgtcta cagaacaata cggtttatac agcacttatc
    16141 tatcgtggga atccttattg ctcatgatta catcgcttag tttgttcaaa gccatgatga
    16201 atctttatgt aaaatatgat aatcaagaac atattctttc gacagtatcc ggattgactt
    16261 tagtactgac tattttttgg tttgttattg ctatttttat taaggatata ttggcagaaa
    16321 ttttaggatt gacaccagtt ctagttgtct gtatgttctc atattttata tttcaggcag
    16381 aaatacaatg ttggtcttta cacaaacgat atatatatga ttataagact ttattagtag
    16441 ctacccttgc agttactgtt ggctcatctg tagttggctg tttagcggtt ctgtttatat
    16501 ctcctaccgc tgaggcgaga gccttaatga atgtcctagt gactggtgtt gttggcgtgg
    16561 ctgtgtatat atcggtattt agaagatgta aggattttaa ggataatgaa gtgtggaatt
    16621 tcgcagtagt attttgtgta tcgttgatgc cacactattt atctgaattt attctgaatg
    16681 gttctgataa aattatgata aattacttat gtggttcgtc agatgttgct atttacagcg
    16741 tggcatattc tattggtgct ctaattaacc ttgttacaaa ttctgtcaat tccacttttg
    16801 caccttatca gtatcaaaaa atcaagtcag gagaatatga tttactatca aaaagagcta
    16861 atgaagttct tggtttggta gcggctatgc tttttgcatt aatgctattt agtaaagaaa
    16921 ttgtgttgat atttggtggg cagaaatata tcgacagtgt taatttaatt gtacctatat
    16981 gtatcggtat ttatttcaac tatatgtttc agctgtttgc tagagtacag gaatactttg
    17041 aaaagaaact cactgtcgtt attccatcgg ttttgtgtgc aattatgaat attgtactaa
    17101 attatgtttt tataaagtta tatgggtatc aggcggtagc atacacaact gtattctcat
    17161 atatgttttt ctgcgtcatc cattatattt tttatagaaa agtttgcaaa gaactattgg
    17221 atggaaaaaa catatatgat gtcagaggaa tacttttgat ttctgca
//
LOCUS       DAFFSV010000052        16840 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFFSV010000052 DAFFSV010000000
VERSION     DAFFSV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.052"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..456)
                     /locus_tag="LR110_001573"
     CDS             complement(<1..456)
                     /locus_tag="LR110_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL4822537.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="LR110_001574"
     CDS             complement(561..1424)
                     /locus_tag="LR110_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL4822538.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="LR110_001575"
     CDS             complement(1620..3719)
                     /locus_tag="LR110_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL4822539.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="LR110_001576"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="LR110_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBL4822540.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="LR110_001577"
     CDS             complement(5072..6913)
                     /locus_tag="LR110_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBL4822541.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9410)
                     /locus_tag="LR110_001578"
     CDS             complement(7095..9410)
                     /locus_tag="LR110_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL4822542.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="LR110_001579"
     CDS             complement(9595..10455)
                     /locus_tag="LR110_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBL4822543.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="LR110_001580"
     CDS             complement(10514..11035)
                     /locus_tag="LR110_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBL4822544.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11313..12074
                     /locus_tag="LR110_001581"
     CDS             11313..12074
                     /locus_tag="LR110_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL4822545.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="LR110_001582"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="LR110_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL4822546.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="LR110_001583"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="LR110_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL4822547.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="LR110_001584"
     CDS             16434..16790
                     /locus_tag="LR110_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL4822548.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4624 a         3493 c         2954 g         5769 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcacta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa agaggctggg acagaagcgt
//
LOCUS       DAFFSV010000053        16792 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFFSV010000053 DAFFSV010000000
VERSION     DAFFSV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.053"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..546
                     /locus_tag="LR110_001585"
     CDS             <1..546
                     /locus_tag="LR110_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBL4822549.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="LR110_001586"
     CDS             735..1403
                     /locus_tag="LR110_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBL4822550.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1989)
                     /locus_tag="LR110_001587"
     CDS             complement(1603..1989)
                     /locus_tag="LR110_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822551.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1961..3343)
                     /locus_tag="LR110_001588"
     CDS             complement(1961..3343)
                     /locus_tag="LR110_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL4822552.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3499..4686)
                     /locus_tag="LR110_001589"
     CDS             complement(3499..4686)
                     /locus_tag="LR110_001589"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBL4822553.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLKKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4693..5724)
                     /locus_tag="LR110_001590"
     CDS             complement(4693..5724)
                     /locus_tag="LR110_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL4822554.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5740..7194)
                     /locus_tag="LR110_001591"
     CDS             complement(5740..7194)
                     /locus_tag="LR110_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4822555.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            7369..8274
                     /locus_tag="LR110_001592"
     CDS             7369..8274
                     /locus_tag="LR110_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL4822556.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8365..9042
                     /locus_tag="LR110_001593"
     CDS             8365..9042
                     /locus_tag="LR110_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL4822557.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9039..9209
                     /locus_tag="LR110_001594"
     CDS             9039..9209
                     /locus_tag="LR110_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822558.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9323..10219
                     /locus_tag="LR110_001595"
     CDS             9323..10219
                     /locus_tag="LR110_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL4822559.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10274..11644)
                     /locus_tag="LR110_001596"
     CDS             complement(10274..11644)
                     /locus_tag="LR110_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4822560.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11882..12628
                     /locus_tag="LR110_001597"
     CDS             11882..12628
                     /locus_tag="LR110_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL4822561.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12625..13464
                     /locus_tag="LR110_001598"
     CDS             12625..13464
                     /locus_tag="LR110_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL4822562.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13465..14415
                     /locus_tag="LR110_001599"
     CDS             13465..14415
                     /locus_tag="LR110_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL4822563.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14556..15308
                     /locus_tag="LR110_001600"
     CDS             14556..15308
                     /locus_tag="LR110_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822564.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIAKSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15327..16478
                     /gene="queG"
                     /locus_tag="LR110_001601"
     CDS             15327..16478
                     /gene="queG"
                     /locus_tag="LR110_001601"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBL4822565.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5465 a         3118 c         3350 g         4859 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttctcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt tgataccgtt gactagttct tccatcgcaa aagcagtccc
     1921 ccattttaat ccaaatggcc gatctcgata ctcgttatag tcaatgaatt tttttgctgt
     1981 ccgtctcatc ttttccacct tctaatccac tcataccacc tgcatgcccg ccaacgagag
     2041 aactacgtgc gatggcgcgt cctccttgca agagactatg cgcatggatg attgattgaa
     2101 agccgtattt ttttctaatc gtatcgacaa caaaatcaac tttcgcttca tttatctgct
     2161 tttcaggatc ttcaaaaagg ttaagctgta aacctgtcgc gtatacgagg tcggaacagt
     2221 tcacgcctac acgccgaata tcctggtagt ggtaatgctg gtggaaaatg gtcaataaag
     2281 catctgctag atctctgcta gcatttgtcg cttcgatttt catttgctgg tgaaacccgg
     2341 tcttgccatt tggatcaaga tagcctaaag agtatcctac ccataagctg actacttgtg
     2401 tttttgctgc agatcgtctc aagcgagtgc cgacttggtc ggccatttct ttgataacga
     2461 tctcgatttc ttctcgtctt gtataatctc tatctaggat ttgactattt ccgatacttt
     2521 ttgattttgg tgtatattct tgtcccaaaa aacttcgatc gatgccccaa gcatgggcat
     2581 aaagctgagt accgatgacc cctagcttgc ttttcaagtg ataataatcc gcgtgagcta
     2641 aatcataaac agaatgtata cccatttgtt tcagacgttt tgctgttcgt ttgccgattc
     2701 cccaaaagtc agtgatatct gagattttcc ataacttttc tggtacatct tcataacgcc
     2761 attcagcaaa catttgcttt ccgtcttttg agccattgtc taaggctagt ttagcaagta
     2821 gcgggttatc gccaattcct atagtcgtgt agattccggt ctgctcaaac acatctttat
     2881 ggatcagttt agccatgtcg tacgcatttg ttttaccaaa gagtgccatg ctatttgtta
     2941 catcaaggaa gctttcatcg acggaataga catgatggtt cgcttcgtct gcatattttt
     3001 tatagataga attgatttct ctgttctttt ccatataata ggccatcctt ggggcaacaa
     3061 tatataaatc acttggatag gggaagggaa gatctctggc acggctgata ttggtgatgc
     3121 cgtaagcttt ttttgctgca ggactggaag ccaagattaa tccgctaccc cgtgtttctg
     3181 tcgaatcgga cggataggac atgactacta atttagttgt aaggggatca agatcctttt
     3241 caacgcactc tacggaggcg taaaaagatt tgcagtcaat acataaaata tctcttgaag
     3301 gttctttttc ataaacaaat accggatttt gattcgccca cataacaatc acctcttcaa
     3361 cacaattaga acacacgttc tctttccgtg tcaataaaaa acagcaattt tcaaatacga
     3421 gcaaaaggct cacatttgga aattgctgag aggttatttt tatctatttg tttttagctg
     3481 agatcgttga tttttttatc aaacatgaac agcttctgga tcgatctttg ttgtcccatt
     3541 tccaatgtgt gcaacataaa attccggagc atagccgaca actttcatgt attcatcata
     3601 gacattgttt ttgaaggcag gtatattttc ttctttgaca agagcaatcg cacagccacc
     3661 aaatccagct cctgtcattc gtgcacctaa gacaccttct tgtttttgag cagcatctac
     3721 tagtgtatct aattcgatcc cagttacttc ataatcatag cgaagagagt ggtgagaatc
     3781 atttaacagt tgaccaaatg cttctaagtt cccagcttct aattcagctt ttgccttcaa
     3841 agtccgctga ttttcagaaa cggcatggcg agctctgcgt atcaatgtat catcgccgat
     3901 ttggtcaata gttgattcaa attcttcttc atctaagtct cctaaagaag agataggcaa
     3961 agtttttttc aaacgtgcca aggcttcttc gcattcgcta cggcgttcat tgtattttga
     4021 atctgccaat tcccgccgct tattcgtatt catgatgacg attgcataac catctagttt
     4081 cactggaacc atttcgtatt tcagcgtatt tgtatctaat aagattgcat gatctagctc
     4141 accgaagccg atggcaaact gatccatgat acctgaattg acaccgatga attcattttc
     4201 tactttttta cctgtttgga ctagttccaa gcgatctgtt tttaaatcaa acagatcttc
     4261 taatacaaca cctactaata attctaatga agcgctactg gaaagtcctg caccattagg
     4321 gattgttcct tccactaaca actcaaaccc atgatcgatt gagtgtccag cttcttttaa
     4381 tttaaggacc attcctttta cgtaatttgc ccagtttgct tttttatcat attctagctc
     4441 atctaaagta aaactaatga caccgatatc gtcaaagttt gtggaataaa ctaatatttt
     4501 attatcgtct cgaggtgctg ctacaccttg cgttccataa gtgatggaag cggggaagac
     4561 atggcctcca ttgtaatctg tgtgttcacc aatcaaattg atgcgtcctg gtgagaagta
     4621 ttgtgtagtt tttttaggtc cgaatgtttc ggcaaatttt gtgtttaagc ttcttttgat
     4681 ttccatgatg agttattcct ttcttgtttt gattttgaat tctgtcactg tttcaaatac
     4741 tgatccggct tttaaatgaa tcgaaccaaa tgctgagaag cgctctgcac ctggagctgt
     4801 ttgagtctca aatgtgatgc caccgtgatg tgccagtttt cggttgcgca tttctggtgt
     4861 ttctgtgccg aaatttgcag tgaaaataac cacactagaa gcatctgtag caatacctac
     4921 ttggattctt ttatcggggc tagtcaagcg agcggcttct ttacccaagc cggtttcttt
     4981 taagaaaaat ggatgatcga ttccgtcaac aagatttttt tgatccaaat cactagcaaa
     5041 aacggacgct aattttttag gtatttgaaa atcgaaagca cttcctgcag cattttcttt
     5101 tacccctata ggaatactat ctgttcgcaa aggagcataa gcttcgctat tgagccaaag
     5161 ctcatgttga tcgattgact gagaagcatc tcccgtcaag ttgaagtaaa catgatttgt
     5221 cggattgaat aaagtatctt ggtcactggt gccgcgagag gtcactcgcc agatattatc
     5281 ttttgtcaaa gtatagcgga tttccacatc cattgtacct ggaaaaccat gttctccgtc
     5341 gggacttgta gtagtaaaaa tgacagaagc ttcattttcg ccattcaaaa tgacataaga
     5401 ccattttttt aattcaaatc caggagcacc accatgcaga ttatgtccag tttttggatc
     5461 tgtggctaat tggtaagttt ttccattgag agaaaagcgg ccattttcga ttctccctgc
     5521 ggtgcgaccg atagaagcac cgatatacgg atctttttcg atatattctt cagcggtgtc
     5581 aaatcccaaa actaattctc gatcatttga ttttaaactg acgattctag cacctaaatc
     5641 agagatagaa agtgctagct tgtttttgtt ctccaaggtt atcaagtgat aaccttggcc
     5701 aaaatctttt tctgtaatct tcattttcaa gatcctttct taatgggaag gaactactgc
     5761 atcgtgaagt tcatcttcca ttgtatcagc tggagccaat ttgttttcca attcctcaac
     5821 gatttgtgca tgttttttct ctgtcaatgt aatcttccaa gcaaaaataa tagcggagaa
     5881 aatcatcaaa gcagcaggag catagaatgc ataagctttg aaagtcgtta cgccatgagc
     5941 agtgatatca gcagcagtag cattaccagt catcccagaa gcgatagcga caaaccctac
     6001 gatcccgtta gacaaagcac cagcgatttt atctagtaaa gggcgaattg atagagtaac
     6061 agcttcgtta cggataccat ttttccattg accatattca acagagtctg tgatcgtcat
     6121 caaagcggat aagaaaatca attgttgagg gaagtagaac aatactagcc cgatgataac
     6181 gattggcaaa cttgaacctg cgatcgtaaa gaaaaagtaa gaagaaatca tcattgcgat
     6241 ccctccaagg taaacgtagc gacgagagat gacgcgtgta agcattggga atagtggaac
     6301 agctactaaa ccaagtaagg cagcaactag accaacgatc caaaattcag ttggttggcc
     6361 aagtacatat ttgaagaagt agaataatac ggcagttgtt gctacattgg caatcgcata
     6421 agcgatataa gcaagggcaa gccacatcaa ctgatcattt tgagcaactg ctttgaacac
     6481 atctttgatc ccagcttttt cattttgctg acggatcgca gtatcgactt cttttgtgcc
     6541 caaggcagtg ataatcgcag taccgcctga aataacagca gcaatcacac cgaaccagaa
     6601 ccagcctgaa gcgccttcaa tatgtttatg agtaacaaga taggtaaaga aagtaacgat
     6661 agggacaacg actagagtag taccatttgc acctagagaa gagccgaaac gagcaaacgt
     6721 agcaaatttt tcacgttcct ttgtttccgt actcaaagct ggaatcattg accaaaaagc
     6781 gatatcctta aatgaataaa aactatccaa taagatgaaa acgattgcaa ataatatgat
     6841 aaataaagtc gtattatgag tagctaagcc aaagaaattg gtaaagattg ctaccaagag
     6901 tacggaacta acaacgccgc cgataactaa ccatggtttg aatttaccgt attttgtacg
     6961 agtattatcg atgatgccac caatcaaagg atcaaaaata atttcaacta agcggatacc
     7021 tacaactaag cttgtaactg tagcaatcat ttttgcattc gttgccttat ctgttcctgc
     7081 gaacatctgg cttgtcacga ataccataaa ataagtgctt aatgtaacat agaatgtgtc
     7141 gtgtccaaaa gcgcctaaag cgtaggacac acgttggatc attttgcttt tcataatctg
     7201 tattcctctc ctgttcaata tataatacta agtaaaccgg ttacaacgaa taacaaagat
     7261 cttttaatga cttaaactta acaagaaatc gcttcctttt taatatattt ctgtgacttt
     7321 tatttaactt tttgttgcat tatactaaag gagtaaggag ggatagacgt ggagcaatcg
     7381 atttttttaa aaaaggaaca tcaacaagta gaaaaagaat tgtattttga tttttgtggt
     7441 ttctctaaaa cgatgccgtt tcattctttt ggtcctgcta ttcgcaatga ttacatcctg
     7501 catgtcgtga tggaaggcaa aggatcttat catgtgaaaa atcaacaatt ccaattgaaa
     7561 aaaggtgatc tgttcttgat tcgtccagga gactcgacgt tttacttatc agatggagaa
     7621 gatccgtgga tgtattgctg gctttcattt ggtgggagtg ctgcagaaga aatcattcgg
     7681 cagtcgcttt tcagagaaga ccaatacacg atggtctctt ctgacataca aaaatatatc
     7741 gacattattt tagaatgcat gaaagatacg gaagaaacct tggaaaatga attacagtta
     7801 acagcattaa cctaccgttt tttatcagtc cttttaaaag atggcggaaa ggtcagcggt
     7861 ggtgtacaaa aggttcattc ctctttagct gttgaaacag tcaaatacat cgaggcacat
     7921 tatgctgaaa aattgtctgt tgaggaaatc gcccagcatt tagcggtcaa tcggagccat
     7981 ttgtcgcgag tattcaaaaa tcatatgggt accagtatca aggaatattt gatcggggta
     8041 cggatcaatc gaggggcgtt tcttctttct ctgacggatg aatccgtgga aagtattgcc
     8101 tatcaagtag ggttcaatag tctagtcgta tttagccgaa tgttcaaaaa gagtaccgga
     8161 gaaacggcta cgaactatcg taaacgtatg aaaaacgaaa attttaagaa tattgctttg
     8221 gataccttga aaaaacagtt ggaaaaacaa accgcagttt cttggacaac ctgattagtc
     8281 tggttaaaat gacaagcatc ggcaaaagaa acttgtggcg gttatgctac actgtaaagc
     8341 gaagactaaa aaggaggaaa agggatgcgt acacttatca tcattagcca tccaacgatc
     8401 gaagaatctt taaatcagca atttttcaaa gaagcttctg ctggtttacc agctacttgg
     8461 catcatttgg aatcaagtta tccagacgga aaaatagata tagagactga actacagctc
     8521 ttggcccagc atgatcggat cattttccag tttccgtttt actggtatag ttctcctgct
     8581 catttaaaaa tttggcaaga taatgtcttg gaacgagcag cacatgtatt aaaagggaaa
     8641 gaactaggaa tcgtattgac aacaggtgtg gcggaaaaag aataccaagc tggaggaaaa
     8701 gaagaatata cgatttccga atttttgagg ccgtatcagc gaatcgcaaa taaattccat
     8761 atgacctatc ttccgccatt cgttttagca caatttatgt atttatccca ggaaaaacgt
     8821 tgggagaagc taattgctta tcagcagtat ctctcattgg aaggcaaacc atcgctgact
     8881 cagcggattg actggtttat tcaaagagta caggaaaatc agaaaatgca ggaagaagaa
     8941 tctgaaaaac aagcatatat cgtagaagca ctgactgagg caaaagagca gatcgaagac
     9001 ttgaccttta cactgcaaga aatgaaagga acttcattat gacaaatgat tggctgatcc
     9061 aacaattaga tgaattaagc gaaaaatcag aatatcaaga ccgtgcgctc ttatatgaaa
     9121 taaagaaact gatggctgag atagacgaac gaacaatgca gctgcaagga gaaatcgacg
     9181 gacaatcttg gaatcatctc aactggtgaa attttcttat aaataacaaa gataaaaatg
     9241 tgcacagcga caattatttg ctacaataag aatatgaagc gtttgataac gctattatac
     9301 ataaaaattg gtggtgaaaa aaatgtatga tgaaaatgag tggggagaaa atgttcggat
     9361 gacccgaaga agacagcaaa aacaggcagc tcgtaagcgg atgatcatga ttggggcagc
     9421 agttgccgtg tttttgatca tagcaggagg agttgcgggt ctatattttc atggaaaaaa
     9481 ttcgtcagaa gcgaaaagta cgaccataac ggaaacaacg acaaagaaaa cagagacgaa
     9541 acaaacaagt gagtcatcaa ctaaaacaaa agagacacaa acttctgaag aaaaaacagc
     9601 aaatggtttg ccagagcaac ttcaagcaat cgtaaatgat tattcgaaaa agatcgaaga
     9661 aaagacgcct gcattgattg aagaatacca gacagaaatt cagggaaatc aagagggaat
     9721 agctggttta tcagcagttg ctaaccaaaa agcacgtgag cttcaagcaa tctccgacga
     9781 agggatcaga aaattacgtg cagcgtatca ggcagcagaa aataaagacg gtgtagactt
     9841 agatactttg atcaatcaat tatcggctaa ttatacgaat catgtcgcta agatttccga
     9901 tatttatttg cagactagcg cctctcttca agcagagtca acaagcacac aagacacaac
     9961 atcttctgat tctgagacag cagagtcggt agaagcttct gaaatgcaag ctcgaacaac
    10021 acaagacaca acagattcct ctgaaactaa taccaacagc gcgacttcac aggctgcgac
    10081 aaccgtagtt ttggaaggag aaggtccaaa tcaaatagcc gaacgaactg gcgtaccagt
    10141 agaaacgatc ttgtcgctga atgggatgag catggagaat tatttcctga ctcccggtga
    10201 gacgctaaaa ttgaattgat aaaagcaata aaaaagaacg aagcttccac taaggtgaag
    10261 cttcgttctt tttttatttt ccggttcttt tttcgcgtac aaagtaatgg atcgtcatgt
    10321 aaagcaagaa aacgagtcca ataatcaatg agattctcgt ttcaggattg ataaacatga
    10381 aaatcaatac gatagctaaa aaagcaagtg tcacataatt cgtataagga gatagtggca
    10441 ttttaaaagg atgatcatgc attttatctg cgtgcttctt tttgaagcga atctcgctga
    10501 tcaaaatcac tacccaagga accataccag gcaaaatact tgagctgtat acgaaaacga
    10561 aaatattttc tgactggttg atgaacattg gtagaagata atttaagatc agaccaatta
    10621 agattccacc tgagattgct acgacaggat aaaaaggcgc accgtgacga gatagtttca
    10681 gaaatttttt agacatcttt ccatttatgg caagtgtata gaccatacga cttgcgctga
    10741 aaatccctga attgcaacct gataatgccg ctgtaatcac gacaaaatta atcagtcctg
    10801 cagcaaatgt aatgccgact ttagaaaaag tttgtacaaa aggtgaacca atctcactca
    10861 attgattcca cggatagata ctcacaatga taaaaatagc accaatataa aaaatcaaga
    10921 ttcgaccgat agtggactgg acagcttcaa tgatcgtctt tttaggattc tctgcttcac
    10981 cagcagtcat tccgatcagt tcgattcctt gataggaagc aacaacgatc gataatgcaa
    11041 agaaaaatcc tttgacgcct ccagtgaaga atccaccatt tttccaaaga tttgaaatcc
    11101 ctactgcctg accatgatta ccaaatccaa ataagattac cccaagacct gcaatgatca
    11161 tcaaaatgat cgttatgact ttgatgatcg aaaaccaaaa ttctaattcg ccaaacgcct
    11221 tgacagaaat aagatttgcc agcataagaa acaagacgac aatgattccg ggaataattg
    11281 ccggcaaatc aggccaccaa taattcatat aagatcctac agcaatcact tcacttattc
    11341 ctacgacaat atattgaaaa atattactcc aagcggtcaa gtagccagcc acaggatgga
    11401 tgtactcgct cgcgtaattt gcaaaagaac cagtcgaagg atcaacgtaa agcatttcgc
    11461 ctaaagcacg cataatcaaa tacaaaaaga atcctgcaat cgcataggct cccatcactg
    11521 agggccctgt ccaacgtatg gttgaggagg cacccataaa cagaccgaca ccgatcgttc
    11581 cacctagtgc gatcatttgg atatgacggg cactaagact tcgagttaac ttttcttctt
    11641 ccatacaaaa actcctttcg ttgttgagtc aacaagaaaa aataatcaaa cgtttcaaca
    11701 catgtttttc attattaggt tagccttatt gaactcttga tgtgattcta cagaaaattt
    11761 tcataaaatg cgaggaaaat tcgaaaaaaa ctttttcatc attctacttg aaaatgcttt
    11821 ccattttagt taaactatat ttaaataaaa agttaggtaa acctaaaaag gagtgtaaga
    11881 tttggaacgt acggccattg taattcaaga cctttcagcc gcttatcaag ggaaaactgt
    11941 tttgaaaact atcaacttga cgataaatcc acaaaaaatt accggtatta tcggaccaaa
    12001 cggtgcagga aaatcaacat ttatgaaatc tttgctagag ttgataccag ctactactgg
    12061 taaagtcacc ttttctggac agccggtaaa ctcagtccgt aaaaaaatag cctacgtgga
    12121 acagcgaagt gaattggatc tttcctttcc aattgatgta ttaggcgttg tacttttagg
    12181 aacatatcca tctttacgaa ttggacaaag acctgggaaa aaagaaaaag aacgtgcaag
    12241 acaagctttg aaaaaagtag ggatggaaga atatgccaaa agacagatca gcgaactatc
    12301 gggtggacag cttcagagag tttttattgc aagagctcta gcccaaggag cagaatggat
    12361 ctttttagat gaaccattcg tagggattga tgcgttaagt gaacgaaaga tctttgacat
    12421 cttgcaggaa ttgaagaatt caggaaaaac gattttgatc gtccatcatg atcttcataa
    12481 agtagaagaa tatttcgatg aggttattct tttaaataaa cagctgatcg cttccggtcc
    12541 agtacaagaa gtctttacat cgaagaacct tcaattggcc tatggtgaga tcatcagaca
    12601 tttagtaaaa ggaggagaga aaaaatgatc caatccttta ttgatggatt aatgaattat
    12661 caattcctgc aatacgcttt ggtcacttcc atgcttgtag gacttgcttc tggagtcatc
    12721 ggttctttta ttattttacg agggatgtct ttgatgggag acgctatctc tcacgcagtt
    12781 cttccaggtg tagccatttc ttatatgttt ggttttagtt acatctttgg agccactgct
    12841 tttgggatgc taacagcagc agtgattggt ttcgtgactc agcggagccg attgaaaaat
    12901 gatacagcga ttggaattgt ctttagctcc ttttttgcat tagggattat cttgatttca
    12961 tatgcccaga gtgcgacaga tttgtatcat attttatttg ggaatgtctt agctgttagg
    13021 gaaagcgatt tgttgctgac agcactcgtt agtggaatcg tcttgatatt tgtctttttc
    13081 ttttataaag aattgaagat cacatcattc gatccgacaa tggcaaaagc ttatgggctg
    13141 aacacatcgc tgatccatta tcttttgatg ttctttttga cattagtggc tgtagtcagt
    13201 ttacagacag taggaacaat cttggtgatt gccatgttga tcacaccagc cgccacggct
    13261 tacttgctaa cgaaccattt actgaaaatg atcattacag ctgcaggaat cggtatgcta
    13321 agtgcagttg tcggtgtgtt tttcagttat agttacaatt ggccatcagg agctacgatc
    13381 gtgttagcat gtaccgcatt ttttatcctt gcttttttat tttctccaac aaaaggaatt
    13441 ttatttcaga aaggaagcag atagatgaaa acaaaaaaat cactcttttt aatcttggca
    13501 gttagtttct tggtattagc cggttgcgga aagcaggctt ctgaccaagc ggatgaagga
    13561 agtaaagaaa agttgtcagt cgtggctacc aattcgatct tggcggacat ggcaaaagag
    13621 gtcggaacag atcaaataga tatccacagt atcgtaccag tcggaacaga tccgcatgaa
    13681 tatgaagtac taccagaaga catcaaaaag gcaagtgatg cagatgttat tttatacaac
    13741 ggtttgaatc ttgaaacagg taacagctgg ttcgataact tgatggaaac ggctaaaaaa
    13801 gaagaaggaa aagattattt tgcagttagc aaaaatgtag aacctcttta tttaactagc
    13861 ggtgaagaac atacaaaagc agatccccat gcttggctag acctatctaa cggaataaaa
    13921 tatgtggagg aaatcgcacg tatattctct gaaaaagatg cagaaaatgc gacactctat
    13981 aaaaaaaatg cagaagcata tgtggaaaaa ctaaaagaat tagatacaca agcgaaggaa
    14041 agttttgctt ctatcgaaga gaacaaaaaa ttattagtaa caagtgaagg ggcattcaag
    14101 tatttttcac gagcatatga tttgccagca gcttatatat gggagatcaa tacagaaagt
    14161 caaggtacgc ctgatcaaat gaaagcgatc attgatcaga taagagcaaa agaagtaccg
    14221 gttttattcg tggaaaccag tgtagactca agaagcatgg aacgggtagc aaaagaaacg
    14281 ggtttgaaaa tctatgataa actgttcact gattccatag caaaagaagg agaacaagga
    14341 gattcttact atcagatgat gaaatggaat attgaaacga tccatgaagg tttgtcacaa
    14401 acaaaagaaa gctgaaaagt gcaaggtcta gcttgaggag cagaccttgc attttttata
    14461 agagatatga aaaaaaatta tgctgttttt cgtcgtacag ataccttttt tgaagaaatt
    14521 ccgttatagt tagagtaagg aaggaaggtg tggctatgaa ttttacacgt aaagataaat
    14581 atacgattaa agaagaagtc tttcgattga tcagccagca ggaaaatacc gacaaggatc
    14641 aagaccttca atttcttgaa gcagaattag ctaaattatt catagcaaat caaaatgaga
    14701 caacagaacc agatgaagag ctgtacatag gcaaatggtt gtttgatgaa tttcagcctt
    14761 tagatgaaaa taccggtatc ggttattttg tgttcctttc tccagtcggc ttcacacctg
    14821 gcgtattagt taaacaaaac gacgaaataa ccgtcaaagc atggcctgca ttattttcag
    14881 tcatcaaagc agaaggagta atcaaaaaga tccagtcggc ctattataaa agtcaattta
    14941 ttgccaaaag caaattaatg aatatgtttt ataatcgtct agtagaaatt gctaggcctg
    15001 ggaaaatccc gcaaacattc acgtacacac agcaaaaaaa tctctatgaa gaagtattgt
    15061 attcccgcga tctgtctgag ttttcgccat gggaagaggt aaggctccat gaactgttaa
    15121 gtacaaaacc agtttcattg aattttttta atgcattaga taacaaaaga aaattaattt
    15181 caacaaatta ttgggaagta tttgctgcaa gaaaaaaatg gatggaagaa aagaagattg
    15241 ccgatcagca gatccagttt gtccagctga aaaaagaaaa actatattat gggttataca
    15301 gccgttaaca gcaagcgagg aagaccatga cattaaaaga agaaatcata caagaaagca
    15361 aacgattagg aattgacaaa atcgggttca ccacggcgga accttttgat tcattaaaag
    15421 aatcgttgga agaacaaaaa gcagctggcc atacttctgg ctttgaacat ccaaatatcg
    15481 atgagcgtct ttatccagaa atgacatttg atcagccgaa atcgatcatt tccattgcac
    15541 ttgcgtatcc tactaggatc catgaagaag tgcctaggga tgaaaaacgc gggcagtttg
    15601 ctcgtgcttc ttgggggatc gattaccatg atatactcag agaccgtttg aaccgattga
    15661 tcgaattcat ccaaacaaga gcagaaaaat ggcaaaaaga agaggaatgg cgtctagctc
    15721 cgcaagtaga tactggagaa ctgatcgatg tcgctgtagc acagcgagct ggtttaggat
    15781 ttattggacg aaacggattg ttgatcactg aagagttcgg ttcatttgtc tatcttggtg
    15841 agatcgttac taatattgta tttgagcctg ataaaccagg agaatttggt tgcggtgact
    15901 gtatgcgctg cgtgactgct tgtccgacaa aagcattgct tggtgacgga agaatgaatg
    15961 cccagcgatg tttgtcttat caaactcaga caaaagggat gatgccggaa gaatatcgta
    16021 aaaaaatgcg aaatgtcatt tatggatgtg atatctgcca gttagtctgc ccttacaatc
    16081 aaggaaaaga ttttcatttt catgaagaga tggaaccaaa agtagacgaa gtatatccaa
    16141 aattaaagcc aatgctttcg atgtctaata aagacttcaa acagcaattt gggcatttag
    16201 ctggttcatg gcgtgggaag aaacctttgc agagaaacgc attgattgct ttagctaatc
    16261 taggtgatcg aacggcattg cctgagattt cagaatgttt aacagatgtc cgccctgtca
    16321 tcagaggaac agcagcttgg gctattggac gtctaggaac aaaagaaccg gaaaaatggc
    16381 ttgatgtatt agatgaaata ttagctaaag aaacagaaga agtcgttata aacgaattgg
    16441 aacatgccca gaaattattg cggagaaagt taaaataaaa aaatagttaa cattattcaa
    16501 agtttttgtc cagtaattgt tgtaacaatt actggttttt ttgtattttt tacatttttt
    16561 taagataatt tgataaaaat caaaaatttt tttgcttttt tttatggatt attgcagtga
    16621 atgttacaga atctgcgttt ttcattcaaa aataaaaaaa catagaaaaa cgattacttt
    16681 gcttatacaa caaggctttt atggttttgt atcaaaaaaa tatttgttta aatgttacaa
    16741 atattaaata attcgttatt ttgtcattaa atgtcatgtt tcttttacct at
//
LOCUS       DAFFSV010000054        16758 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFFSV010000054 DAFFSV010000000
VERSION     DAFFSV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.054"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..189
                     /locus_tag="LR110_001602"
     CDS             <1..189
                     /locus_tag="LR110_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL4822566.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(260..1570)
                     /locus_tag="LR110_001603"
     CDS             complement(260..1570)
                     /locus_tag="LR110_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL4822567.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1582..3000)
                     /locus_tag="LR110_001604"
     CDS             complement(1582..3000)
                     /locus_tag="LR110_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4822568.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3240..4808)
                     /locus_tag="LR110_001605"
     CDS             complement(3240..4808)
                     /locus_tag="LR110_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL4822569.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4812..5093)
                     /locus_tag="LR110_001606"
     CDS             complement(4812..5093)
                     /locus_tag="LR110_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL4822570.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5090..6013)
                     /locus_tag="LR110_001607"
     CDS             complement(5090..6013)
                     /locus_tag="LR110_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBL4822571.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6010..6888)
                     /locus_tag="LR110_001608"
     CDS             complement(6010..6888)
                     /locus_tag="LR110_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBL4822572.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6875..8212)
                     /locus_tag="LR110_001609"
     CDS             complement(6875..8212)
                     /locus_tag="LR110_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL4822573.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8215..9318)
                     /locus_tag="LR110_001610"
     CDS             complement(8215..9318)
                     /locus_tag="LR110_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL4822574.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9392..10900)
                     /locus_tag="LR110_001611"
     CDS             complement(9392..10900)
                     /locus_tag="LR110_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL4822575.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10866..11558)
                     /locus_tag="LR110_001612"
     CDS             complement(10866..11558)
                     /locus_tag="LR110_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL4822576.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11580..12554)
                     /locus_tag="LR110_001613"
     CDS             complement(11580..12554)
                     /locus_tag="LR110_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4822577.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12854..13726
                     /locus_tag="LR110_001614"
     CDS             12854..13726
                     /locus_tag="LR110_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4822578.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14308..16506)
                     /locus_tag="LR110_001615"
     CDS             complement(14308..16506)
                     /locus_tag="LR110_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4822579.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
BASE COUNT         4964 a         3503 c         2721 g         5570 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata tcattaaaaa aaataaaggt caagctaaaa tattatttaa
      241 gcttgacctt attataattt caacgattgt caaaagtatt gtttgcagct gtgttgccag
      301 tttctataga taaatcacag cttttgaaac ataagttttg tactgcttcg attcgcccga
      361 attcttttgc agttatttgg caatgagaaa agcagacatc ttctataggc ttttcaggaa
      421 atgctactaa atcaaaagct cgggaaggaa gtgagtaacc tggagaaagg catgcctgta
      481 cgtttttgat gtgtatgtct ttgactttcg tcatttgctt tgattcaggt atttcttcag
      541 ccactgcttg ccaactttct ggcatttgag caaggtcatt gctatttttt ctattgtatt
      601 cattatgcca gtccattatc caagaaaaag gaaattgtac atcaatcata ttcagatttt
      661 ctaccctgat attttcgatc actccccctc tttcttttga agatttcatt cgaataccac
      721 agtctgactg aaaaaaattg atatcgtgaa tatatacgtc actaactcca gcactaactt
      781 cgctaccaat agttacaccg tatcctgaac ggattttaca tctagcaacc tcgattcttg
      841 atgattttcg attgactctt gcaccattgc catctctgcc agacttaata gcaatacaat
      901 catcaccaca ggccaaatca cattcataga cacgcacatt tgttgaagaa tcaatatcaa
      961 tcccatctgt acttggtcca tcattgttgc ggatgattac tttttctact aatacatcgt
     1021 tagaataagt gatttgcaag ttccaaaaac ctgatttctg aaaaatcaaa tcacgaacaa
     1081 caacatgttc tgcatgatac agtaaacaag cgcgtggacg tttaatagca taatccgcaa
     1141 tccaacgaag tcccttttga tcgtattcag ctcttgtccc gcttttttga tcttttcccc
     1201 aatacagctc ccaccaatgg tctccttgac cgtcgattat tccttttcct tctataagaa
     1261 tatctttcgc cgagagaaca ttcaaaatcg ctgctggcca tttcatttct acgccggcta
     1321 ctcgtgtatc gatttctgga aatgcttcga tatctgttct ccccttgagg actgcacctt
     1381 cttcaaagat aagatgactc ttatctttta aaaaaagggc tccgactaaa taaatgcctt
     1441 ttggaataaa tacaggtaag tgttctttcg caccttgatc gattgctttt tgcagtatta
     1501 ttgtatcatc ttgttgagtg tcgtttgttt taaagttcaa agcattgata atcccttttt
     1561 ttgtccgcaa tacgtttctc cctatctttc attgattttt ttcaattctt caccaataaa
     1621 tacagttttt gaacgattca gtgggtaacc tactagcaaa atgattgcgg atacaaataa
     1681 gcagaatgct ggtaacaagt ttgccatcgt ataaatcctt tcttgtacta aaactgattg
     1741 gatggctcca cctgttgtag acgattggta accaatgagt gccagcataa atccgcctac
     1801 cgctcctgca catgcttgtc ctacttttct cgcaaaagaa tttactccat atactgttcc
     1861 atcttctctt aagcctgtct tatattgatg ataatcaatc acgtcagtaa tgaatgccca
     1921 gaccatcaaa ttgaataaac cagatcctaa agtagcgata aataaaagaa tcagatatac
     1981 ccatgcattt gttatatgaa ggaaatacat cagcaagtac atacatgaag aagcaagtaa
     2041 cgcaaatacg cttgcttcct ttttaccaaa taattttgtc agctttgtag caaatggtgc
     2101 taaaactact actgttccgt aagtaaataa aagagcaaca gacattgctt gtttattatg
     2161 gaagtaatca ttgaataaat aagtcatatt cgtcccagct aaaatctgat tgatcacaat
     2221 gaaaatgtca acaatgacta aaataatcag tgctcgatta gctaacaaac cttttaccaa
     2281 ctgagaagca gatacttttt cgtttttgac aactcttact ctttcagtag tcaatttaca
     2341 agttaacata tatgcgacaa atgctattgc agcacatccg attgaaatcc aaaagaaatg
     2401 attccctgaa acgacttgtt gtccactctt gttatgggta tacattaaaa tcggaatgaa
     2461 gaatcctgta gttgatccgc caatcgcaga accaatgctt cggaacgtgg acaacgaggt
     2521 gcggtcatta ggatttgagc taattgctgc tgccattgct ccatacggga tattgattgt
     2581 tgagagaaaa atcccataaa caatgtatga aacaaaaata taagcaatct tcaccgtcat
     2641 tggaagatgt tggacgaatg gcaaaaacaa aatgacagtc actaagcaaa atggatactt
     2701 catttttctg atccaaggaa taaatcggcc agtttctgtt aactctgaga catcacataa
     2761 tcttccaacc atcacatcag caaacgcatc caaaattctc gaaatcaaga ataacgtacc
     2821 tacgattgcc cctgaaacgc caagaacatt cgtataataa atcatcaaga aactattgac
     2881 taaacctaaa ataaaacagt tacctagatc accgaacatg tagcctatct tatcggaaat
     2941 cccaaattcc tttttttctt tttttactgt atttgctgta acagcatttt gtgaaagcat
     3001 ttgaattcct cctacctctt ctagtaaacg ttttcaaaat tataactctt tttttcttgt
     3061 ttttatattc acaatgttgg ctttttattg cattatcttg acagataaaa aacaaaaaat
     3121 aatctaaaaa cattgatgca taagattgat aacttttttt acataacaat atatacaaac
     3181 aaaaaaattc actaactagt gaaaataagt tagtgaacct catttattaa agaattaaat
     3241 tacagatgat cttttccatc atacttgaaa aactcaaaat cagcataact tcctcttttt
     3301 ttatttagat catgcactgc tatcccgata aaatttccag taaaccctcc tgagagtata
     3361 cacatgtttt tttgcggacc tactttcttc cattcttcag ttgatacgtc tttcaaatga
     3421 aactgtgctg tctctttctt tacagtgact ttcaaaaaag tcttcttttc tgtgagtgga
     3481 atcgtccaat cttctatttg tgctactccg ttttctgatt ctatcatacg taggcatttt
     3541 cccttgtcct cattccaagt aacataacaa taaatataat tggattcatt taagtacagt
     3601 accagtccag ccatctgatt aaaattatta ggcatgtatt caaatgctgt ctccgcatca
     3661 aaagtaaaat ccgtttgccg aatagctaac aaatgatgtt cgaaggtaga ttgaagagat
     3721 tcaccggaaa tcagacgtaa aaaacctggt ctacattttg tatcacacca atcagcattt
     3781 ggcaaaatgc gtctggcatt ccagttcttc tttagctctc cttcaaatgt atccataaac
     3841 tgatgatctt tttcttgttg tacctcttca tttgtctcaa tcattatttc ttccatcgga
     3901 gttgtctggc tatctttaag tcttaaccaa tcgtcggacg tccaatacac ttcttgaata
     3961 gcggtttctc ttcccaaaat agccgcaccg tttaatggtc tggtacaaag atatgtcatg
     4021 taccatcttc ctgacggggt ttgtactaag ctcccatgtc ctgtacattg caatgtagaa
     4081 gcaggtttgt cactagctgt cagcatagga aaaccaggat caagctcgta aggaccagta
     4141 atattctttg ctcggcaaac tgtaactgaa tgtcctcttc ctgtaccgcc ttctgccgtc
     4201 atcaaataat aatacccgtt acgaaaatac aaatgtggtg cctccgtttt cgctaattct
     4261 gtacctgcaa atattttata gatgggacca tcaagcttct tcttctctgc atcatattct
     4321 tgtaatacga taccgctgga tttattcggt gtgtccagac gataatccca tagttcattg
     4381 acaagccatt tttttcccgt ttcaagatca tggaacaaag aaggatcaaa gccgttacta
     4441 tttaaataaa ttggttcgga ccaaggacct gtgatctcag atgcagtgat caaataattg
     4501 tggacatctt taaacggacg gcctgcactt ttgacatctg tgtaaacaag ataaaacaat
     4561 ccatcagcat aactgatctg tggcgcccat atacttccat ttgtaggatt tccttgcaaa
     4621 gatacttgag tggtcaaaat atccgttgta tgtgaccagt tgactaaatt tttcgactca
     4681 tatactcgga ttcctggaag ccattcaaaa gaggagacaa taatgtaata ccgctctttc
     4741 actcttaata tattaggatc tggattgaat cctcttaaaa taggattttt cactttgata
     4801 gtctgcatga ttcaggctcc ttttctcatt cttactcgat ataaactgaa tgccccagct
     4861 gctaaaaaag caagtgaaat aaacaacaca acggcaaaca gaccagtcat gtcctgtaat
     4921 aatccaaatg tattttctaa agacaaagtt gaatgatcta cagcaagcga ctgataactt
     4981 tttttaaact ctgacgcaga catattactt aaagtaactg ctaacccgcc aaagaaaatc
     5041 aaagatagac tgccaccgac cattcctagc aatagttccc attttctttt catttctgaa
     5101 ccccctgctt ttctaaatgt atccattcag gtcttttgat cgatgtttca taattgttat
     5161 tacgttcaga gaatttgaca gtctgttcat ttttcttgtc atcccaatta tcccaaccag
     5221 ttgggtgaac atgcccacct atcttacacg cttcaaataa gacatttgca tacggacgcc
     5281 acggtcttcc taaatagacg tttctagtat caggttctgc agtcaaggta cagctttcaa
     5341 aacgataccc tgttttttga tttttcggcg tagatgctgc tgtgatgtat ccaggatttt
     5401 tactggctaa gctatggata tgacactgat gaaaaacggc atctgcccca ccaaatatga
     5461 aatccaccgt tccttcaatc cagcaatatt cataggtctg acgacaatac tcaggattgg
     5521 cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa atcaggcaac ggacccgtgc
     5581 ataaggtatc ctgataacca gatagtcgac agttttttaa agatacgcga tctcctgaat
     5641 tgaataaagc aacagcctgt cctacctttt ctcctggacc tgctgtattt tcgatgtgaa
     5701 gattttctaa tacgacattc gatccttcta agaaaaaagt agcacttcgg aaagtcccta
     5761 actggctacc gtcaagatac tcctgttttg caaaacgatt accggagata actactgttc
     5821 ctgctcccac aagtttaaaa ttggataatc tgctttctat atattcatca tatcttcctt
     5881 cttttactat caagaccttt ggatggctat ccggcaggcc tgccaaataa tcgattcctt
     5941 cttggatagt ttcaaaatcc gtcggggtat ctatagcatt ctttccaatt atcagtttgt
     6001 ctttcatcct cataaatgct cccttatgaa ttgtttccaa atatttctca tttcttgtgt
     6061 ttccatatga ccgcctgtta aagaaaaaga agaaaagttt tctggactac ccattgattg
     6121 atactttttg tccagccctt ttctcagctt cagtacgcct tcgtccaagc acatcgtatc
     6181 gttttttcca actaagctaa gacgagggcg cggagcgatt ttttcttgga ttgctaacgt
     6241 tgtataagct gtcaatagat ttggcacata gtaataaaac ccatgatgat ctaggcggcg
     6301 atgtagcaaa agtgtttcga tgtccacctg tgccgctaga tcaatacaaa ctttgacacg
     6361 agaatccaat gctgtcaccc accagcttaa taagcctccc atagaaagac caatcgttgc
     6421 gagccgttga gtatctgtgt cttcccttgt ctccaaataa tcgatcaagc tgatcacatc
     6481 ataaatacgc attccccata acgtttttcc tgttaataaa aattgtttga acaactcgct
     6541 ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca atcgaccaaa cagcatatcc
     6601 cattcctgtc agtacttctg caaaagatgg agaagctaag taaggagcac catggatcaa
     6661 ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc aggtaaagga ccgtcggaaa
     6721 cggtccttct ttttctggat aagcatagag ggctggtact gtttccaatt catttaagta
     6781 caattgatag tattcaagca taaaaccgtt ttgtttttct cggtataatc gttttccact
     6841 aggggtatgt ctgctagatg gagcacctaa aaaatcattc aactgcataa gcaaagtttt
     6901 tacgaacaac cagttcatcc atattttctg attgatttga ttggaatttc tcaatactga
     6961 tttctttagt attttctaat agaaatgctg gtccctcgtg attttcaatc gacatctggt
     7021 taaaggaaac cttacccgca aagccaatat aaaatccttg tctgctcatt tcctctattc
     7081 ctgtcatcat agcaggaaca ccgggaactg catcttgggc catcgagaca tcgatattgt
     7141 caaatgtgat ttcttgtatg aattgttccg ctaatccata aataaaacct gcagaagcat
     7201 ggacattcct tgccgtgata ttcgaaaaat ggatccttct gaatgctgat gtccgttcat
     7261 ctatcggata agcctttttt tcccatacat acggttcttt ccctcgcgga ccacagaaat
     7321 aataaagatt cagtataaat ggacacatca cattatccat gactatattg gatactcgaa
     7381 tatcttcaac gatcccgcct cgacctcgtc tagatttcaa tcgtattcct cgatcagttt
     7441 cttggaagat acaatttgaa atggtaatat tacgaatact accactcatt tcacttccta
     7501 atacgacacc gccatgtcca tggaccatcg tgcaatttgt gatcgtgata ttttcgcaag
     7561 cgatcctttc gtacgtatct tcagtccctg ccttgattgc aatacagtca tctcccacgt
     7621 caatatggca attactaata cgcacatttt tgcatgattc tggatcaatt ccgtctgtat
     7681 ttggtgaatc cgctgggttc aagatcgtca agttatcaaa cgttgcatta ctgcaaagaa
     7741 tcgggttgat cgtccaactt ggtgactgga tcaatttaat atctttcaca gtaattcgat
     7801 gacagttatg gaaactcatc aatttaggac gaggataagc gagattatct ggttcatttc
     7861 taaaagtatg ccaccatttt tttccatttc catctaatgt accgaaacca gtgacagaga
     7921 tgttctctac attttgagca tagatacagg aagcataaac ttttcgatgg actccttccc
     7981 aacgtgaatg aacaacagga taatcttttg gatcgtcaga gaatttcaga accgctcctg
     8041 cgcttaaatg aagttcgaca ttgcttttta aaaataaagc tcctgttaga aattcacctg
     8101 ctggaacaac tacggtcccg cctccgtcac ttgctgcagc atcaatggct tgttgtattg
     8161 cttcagtatt caattcatca attgatgcgc caaatgtcaa aatatcatac atatttataa
     8221 tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg cctttttgat cattacaaat
     8281 aattggttca ctgatgtagt aagcatatga accatctcgc tgatctgctc cgcctaaccc
     8341 tgctacttga cagtttttat tcaagttcag ccagccttct tttgtttcca gaacaaattc
     8401 atcaagtaat ccttgatgtg atttcttcac ttgtggaagc cattcttctt tgttcagcag
     8461 tcccaaacgt attcctttag ccattgcgta aacaaacata ctactgccgg aagcttccag
     8521 ataatttcct ttccgttttg tttctgttgt tacttgatac catgtcccgc tgttttcttc
     8581 ttgatatgga agtaatgcag ccagtgtctt ttctagaatt tctttcagca aagaacaatc
     8641 tgtttcttca tgaagtaatt cataggtatc tgctgcagcc atcaaatacc agccaatcga
     8701 tcggctccaa aaatgatggg atagacctgt ttttggatca gcccatggtt ggaccttctt
     8761 ttcatcccat gcatgaatca gcaaatttgt ttttggatcg aatagatgtt tataactgat
     8821 ttcaaactgt ctcaaaacat cttggtaatc tttttcttca ctaaaattct ggataaattc
     8881 agcataaaaa ggagaaccca tatataagcc atccaaccaa atttgataag gataaatttc
     8941 tttatgccaa aaagctcctt cggatgtgcg tggatggttt gccaactgct gggaaaggag
     9001 ttctgctgct tttttatatt tttcttcgcc tgtttctttg tacaataaaa agaaaagctt
     9061 tcctgtattt acatggtcga tattgtattc ttcaacgctg tagccacgga tcgttccatc
     9121 ttcttgcacg aaatagtcga tattgtcttt tatgaaatca aaataaattt tttcttgggt
     9181 tcgttcccaa agtaattgga accctttaag tacgacgcca tagtcataag accattttcc
     9241 tttataccac ttttcttcat ataagcgagg tgttctttcc atgatacttt ttgctaattt
     9301 ctttgaccag ttttccatta tttgctctcc tttaaaaatt aaaataggat ttgggacaaa
     9361 taccaagaag tatttgtccc aatcgtctat attactgtgc cgctgctttg tatgcttcct
     9421 cgtactcttg tgtgattcga tcgcctcctt gtgctttcca gtcttctaca gcttttttga
     9481 atccatcttc gtcaagctgt cccatgatgt attgcaccgt agcatcaatg acgatttttt
     9541 caatttctga tcctacttca ttgttcgtcg gtgaatccaa tggcacagaa ggatccatta
     9601 cagcaaattc ttcattttct ttgatcaatt gattagagaa ttctttttca gggtctgtat
     9661 ctttgaaaga gaacgtcact tcactaggac gagagcttga taacggttgc acctcttgtt
     9721 gccaaagatc catatcgata aattcgatag aaccatcatc attcaattca taatgcgttc
     9781 cttcgatacc gccagtcatc aatttataca catcttcttc taaaagatca tcaacaaatt
     9841 gaagtaattt cttcaattgt gcttcgtctt ttacttgaga tttgggaaat gctagcaagc
     9901 cgccaatacc atttccttct gaccaaacat gataaccgcc gccttcacca tctgagatct
     9961 tgtttacagg cactaattcc atatcatctt gaataccttg acccatcgtc ttcagattct
    10021 tgatatccac cattccagtg tagattccag ttcttccttg tgcaaattgt tcttgctgat
    10081 cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc attctcaaat agatctcttg
    10141 aaaaattcaa agctttcata tactcttctg tatcaaattc tggagtgaat ttgccattat
    10201 cgtctacagc ccagccattt ggtgctccaa aataagaagt gaaaagttta aagctggtat
    10261 aacgaatatc actaggcgca cgatcgccaa aaccaactgt gtcgtccaca ccatttccat
    10321 caggatcgtt ttctgtaaat tgtttagcta cttcataaag ctcatccatg gtagttggaa
    10381 catctagtcc tagattatcc aaccagtctt ttcgaatgac taagccagca cgagcgtagt
    10441 ctttttgtat cggtacgccg tacaaggcac cgtcgatact cgcagcttcg attctatctt
    10501 cagaaatttg acttaaattt ttatagtctt ttaaatattc tgatacatcc cagaatacgc
    10561 ctgattttaa cgcattgcgg acagaagagt ttttcaacat cgcaatttga agtgtaacga
    10621 tatctcctag ttcatcagaa gccaatgcag ttgttaatct ttcttctttt gaagcatcag
    10681 gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc gatcaagttc acaatcgtgt
    10741 ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt gattttagct tttccatctt
    10801 ctgtctctga agaactgtta cttgaactgc tgccgcatgc ggcaagtgct agaattcctg
    10861 caaaactaag acctaatttt acgagcgttg cttttttcat aaattttctc cttcactgtt
    10921 ttaataatat ttttgttttc ttctgttttt tctgtatatg agttcttcca ttccaaatgg
    10981 caaaatatag aatttgctgt ccacgtgact gcggaaaaga agagactgcc accgataaag
    11041 gttaaaacac ctggcattgt ttggatgatc atcagataaa tccctaccat acttaatatc
    11101 aacactaatg agtaatgaag atgtgagaat ccgaataaaa atgccatttt cattttgaaa
    11161 aaatatcctt tccaatcata atgagccatg attgggaaaa tatagagtgc tgagagcaca
    11221 tatatgaacg cagcaatata caaagcgatc ctaagatacc aattgtgtat gaaagaaaga
    11281 tccacaagta aaacaatccc gatggcgcca tatatccagc tcatcagcaa ggtttcacga
    11341 tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag gaaacgtttc ttttgagcgc
    11401 agccattttc taatcactgc aaccatcgca tacgatgcag gaccgaaagt aaaaactccc
    11461 aaacctgcga tgactaaacc gccccacaga aaattcaaat aaaccaactg taaaatacga
    11521 tttagccttc cgtttatttt atctaatgac tgcagcatat ctttccctct ctttctatct
    11581 tagtaaacgc cgtcttctcc tagctttttg gcaatcttat tagctaaaat taccataaac
    11641 aatcctacga cccctttaaa caatcccatt gctgtactga aacttaactg tccattcttc
    11701 aatccagctg tataaatata ggtgtcgaaa atttctgcaa cgcttcgatt caatgaattc
    11761 aataacaagt acatatgctc gaaccctaag tctaatgtct gaccgatttt tagaatcagc
    11821 aaggtaatga ttactggacg aattgccggc aatgtaacat gccatgtttt gcgtaagcgt
    11881 cctgctccat ccatttctgc agcttcatac aactgtgtat cgactgcagt tatcgctgaa
    11941 aggtagatga tcgtagacca gcccaactct ttccagatca cttgtccgat atatactgtg
    12001 cgtgtccatt caggggatgt tagaaaactg attttttcaa atcccattgt tgcaaggaat
    12061 tcattcaata cccctccgtc aacattcaaa aatacataag taatcgaaac aatgattacc
    12121 caagacatga agtgagggat gtaaatgatc gtttgaatcc cgtttttcaa tcgtttgttc
    12181 gttacttcat ttaatagtaa agacaaaatg attggcatcg gaaaaaacac gaagatattt
    12241 aatccgaaca agatcaacgt atttcttaat aatgtaaaga atgtcggttc tgtaaacaaa
    12301 cgaatgaaat gtttgaaacc gacccaagga cttcccatta ttcctaaaaa tggttgataa
    12361 tcttgaaaag caataacgag tccccccata ggaagatacc taaaaatcaa gaaataaagg
    12421 actcccggca gaatcatcac atataaaaat ttattcgttt tgattcctgc aattattttt
    12481 tttcgtcttc tgagcttttc ttcttttttc gatagcgtca gttgaaatgg ttcttgtttc
    12541 tttacacttt ccataagccc ctccttagtt ttgtgaatgt ttgctgctta tgacttcatg
    12601 atagataact atctgaatta cgtatataat catttttatc gtaagtaaaa acgcttacat
    12661 agcaatgatt tttgtgctat acaaaacgat aaagtgtatt ttttcacaaa taaactacgc
    12721 atagcaaacc ttctattttt ttctatctga cacttagcat aggaaatgat tatatacgta
    12781 atctttcacc gatgataaat tgaacatata gaaacagaaa catttaattt attttgagga
    12841 ggagcctatc tatatgaaaa agaaagtctc tataggagaa agaagtttta cacttttcaa
    12901 caatacgttt ctaattttgc tagctttgat ctgtatcgtt ccttttttaa atatcatcgc
    12961 tacatctttt gcttctacac aagaagtcgt cgcgaaaaag tttatccttt ttcctactac
    13021 cttttcttta gacgcgtatc gctacattct ttctacgccg accattttta gagcacttgc
    13081 tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt atgtgtgcaa cttcactgat
    13141 ggcctacggt ctttccagaa agtatttgtt tggtcgaggt ttcgtcaact ttcttgttgt
    13201 attttctatg ttgttcagcg gggggatgat tccaacgttc ttagttgttc gttctctagg
    13261 gctggtcaat tcttactggg ccatgatcct gcctgtggct gttaatgcaa tgaatatgat
    13321 cattatgaga aactttttcc aagccttgcc ggatagtttg gaagaatcag caaaaatgga
    13381 tggttgcaca gattttggtg tatttttcaa aattatgctg ccattagctt taccgtctat
    13441 tgctactatc tcgctttttt atgctgttac ttactggaac acttatatga ctgctatttt
    13501 gtatatcaac gattcatcta aatggccgat acagattctt ttacgccaaa tcgtcattgt
    13561 atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg attccaccag cacaaacagt
    13621 taaaatggct gttatcgtta ttgcaacagt gcctatgctg attgtttatc cttttgttca
    13681 gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa gggtgattat gtactgaacg
    13741 caaaatggta gttgtgtcag aagtggtcag cttcaagaaa taagaaggca tttacgaaaa
    13801 ttgcttctta aatttttgtg aaatttcggc ttatttttcg aagaatctac tcctggaaca
    13861 ccgtgtatca gaggttgtga aagagacgtt cagccttgag cgatgataaa aactctcaca
    13921 aacattgcga cgagtaaccc acaggagcaa taagaagcga ttttgaaaaa tagcttccca
    13981 tatttcacaa aattatgcct tattgtcgat agcctgtctc ttgaacgcca tttattcgta
    14041 aaaaagaact gtgacaagac ttttgtcaca gttctttttg cgtgcttagc cttgaaattt
    14101 aactatccat aaatacaaat actagggtta ttctaacttc ttttattgct tgaatcagtc
    14161 cacaagaaaa acagcctctt gattaaacgg cgtttatctt gttgattctg cggcaataaa
    14221 atcagccagc agcaaaacat gaagtgctgt tttttgctgt cctcttttat tgcttgaatc
    14281 agatcacaag aaaaacagcc tcttgattca caaatatttt tttcgatatt ctcccggcgt
    14341 aatcgattcc ttctttttaa agaaacgaat aaaattttgc ggatttcgat attgcaatct
    14401 ttctgcaatc tcttttacgg agaggttagt ctctttcaac catttttttg ctacctctaa
    14461 acgatgattt tgaacaaaat caccaaattt ttctcctgtt tcctttttaa atacattact
    14521 taaatagtta gggttataat gcaacctatc agcgatcaca tctaaagaca gatcacgatc
    14581 atattctgtg tagacgatat gcataatatt ctccgacaaa tttttaaatt catgatcagt
    14641 gattgcttga gagcattctg tgattggcag aactaattgt tctaaaacaa gtttttctag
    14701 ttctttggga tgataattgt tcagtgcttt tacataaatt tgtttcatgt tatcaaagtt
    14761 ttttgaatct actcccaata actgtcctaa ttgaacgagt tcattgatca agcggaccga
    14821 aactacttcc cgactcaaag gatttttatt ttgtgcaaac aatgcatcaa tcaattcatg
    14881 tactaaattc ttcacttcct gatttccaga acgaatggct tcaaacaatc gattttgttc
    14941 ttctatcgga tatctaataa gtgttttttc atgagcagca ggaacgattt cctgataaaa
    15001 aataattgaa cttggaccag tatttacacg ataatacagg gcttcttttg cccgatcaac
    15061 cgattgcttg ctttcattca gcgtttcgaa ccggttactg atgcctatac tgatagttaa
    15121 attcaagtat tcttttacac gtttttgaat gagtcggcaa tactccatga cttttttgct
    15181 tgcttctgca tcatctttat ccattcgata aatagtggct tgcatttctt cattcaaaac
    15241 gatcggcagc attcgatttt cctttggcac aatttccgtg atcatgttgt tgatcgctaa
    15301 aagtagcaaa tcacgctccc caccgtgact atcttctaac agatcgattt gaaccaaagc
    15361 tgtataatag cagtcatttt gagaactata accaaattgc tgcaaccgct gatctagttc
    15421 tcgcctttct actcgatttc taaataaact aagtacaaat agcgtttcca gctgtggctt
    15481 ttgggtaact aagtgtgcac ttaacgcttc tttttctcct atgatccgac tgactgattg
    15541 agcgaccaaa tctagttctt tcccactgaa tccttttgat ttaagattca gacgttcctg
    15601 tatttgagaa atcggacggg caaatctgtc cgaaaataag taggaaaaaa gaccaatgag
    15661 tcctaccagt gttaaagaca ctgcaactag cccgattctc agattgcgga tcattgcgcc
    15721 aattgcacta gagtcaactt ctaatctgta aatccaatta ttataatctg atttcagtac
    15781 tatatacgtt ttatcttttt ctcttatcac tttcagctga ttgtaatcta gatccacgtc
    15841 cgttaataca gttggttgct ctttattttt tactgtctga gtagtaaaaa ggttgccaac
    15901 ttcgttttca atagaaagta tgtcttctcc actttcacca acaacttgtt ctattgtatg
    15961 tttctctatc gtagcaattc ccaatgcata tttttctttt tcaaacattg gcaaagaaat
    16021 caccatttcg attcctttgt ttttcttttg ccaaaataaa ttatttttgt tatttatgta
    16081 cttctgttga tacttttcaa cttcctcttc ggtcagttga ctcaacgatc cattgattac
    16141 tccccattta ccagccaaac tgactaaatc ataattatta tcatccataa caatcgtttc
    16201 tataaaattt agttctttcc tgatttcaga ataagtggag taatctgtat atgtcagcgg
    16261 atgcttgaat atgtccgaga aatttttggt cgaaccatac gtagaaaacg aatattcaat
    16321 cgttctaatt ttccattcaa cgttagtttt aacttgcctg agcaaattct ttttgtctgt
    16381 taaataactc gattcgatcg attcataggt attctggtaa atgaataaac taaatcctat
    16441 gacaagtacg gctcctaata aaaacatcgg taaaaatatt ttataaaata ctgtactttt
    16501 cttcattttc ttcctctcct ccttttaaaa taaaaggctt tcatttttct gtaaaattga
    16561 tactcatcta attaaatgag cattccccgc tttcaagaat agcataatta aaaataaaaa
    16621 tccaagtaat ttttaacgtg tgcaatcttt tcatatcaaa gatctattga cttatcgtag
    16681 ttatcgtaat atgatgaatg cgttaacaaa aagtgaaaaa gtaaggaaaa tgatggaatg
    16741 gtaacgatca aagatatc
//
LOCUS       DAFFSV010000055        16527 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFFSV010000055 DAFFSV010000000
VERSION     DAFFSV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.055"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(141..416)
                     /locus_tag="LR110_001616"
     CDS             complement(141..416)
                     /locus_tag="LR110_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBL4822580.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(659..1969)
                     /gene="der"
                     /locus_tag="LR110_001617"
     CDS             complement(659..1969)
                     /gene="der"
                     /locus_tag="LR110_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBL4822581.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="LR110_001618"
     CDS             complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="LR110_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBL4822582.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3515..4195)
                     /locus_tag="LR110_001619"
     CDS             complement(3515..4195)
                     /locus_tag="LR110_001619"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBL4822583.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4244..4879)
                     /locus_tag="LR110_001620"
     CDS             complement(4244..4879)
                     /locus_tag="LR110_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL4822584.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4945..6348)
                     /locus_tag="LR110_001621"
     CDS             complement(4945..6348)
                     /locus_tag="LR110_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBL4822585.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6335..7366)
                     /locus_tag="LR110_001622"
     CDS             complement(6335..7366)
                     /locus_tag="LR110_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4822586.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7397..7618
                     /locus_tag="LR110_001623"
     CDS             7397..7618
                     /locus_tag="LR110_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBL4822587.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7783..8520)
                     /locus_tag="LR110_001624"
     CDS             complement(7783..8520)
                     /locus_tag="LR110_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBL4822588.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8673..10013)
                     /locus_tag="LR110_001625"
     CDS             complement(8673..10013)
                     /locus_tag="LR110_001625"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBL4822589.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10250..11773)
                     /locus_tag="LR110_001626"
     CDS             complement(10250..11773)
                     /locus_tag="LR110_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBL4822590.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12151..12762)
                     /locus_tag="LR110_001627"
     CDS             complement(12151..12762)
                     /locus_tag="LR110_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL4822591.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12885..13010)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13092..13808)
                     /locus_tag="LR110_001628"
     CDS             complement(13092..13808)
                     /locus_tag="LR110_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL4822592.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="LR110_001629"
     CDS             complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="LR110_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBL4822593.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14367..15164)
                     /locus_tag="LR110_001630"
     CDS             complement(14367..15164)
                     /locus_tag="LR110_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBL4822594.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15134..15505)
                     /locus_tag="LR110_001631"
     CDS             complement(15134..15505)
                     /locus_tag="LR110_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL4822595.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="LR110_001632"
     CDS             complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="LR110_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBL4822596.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3577 c         2798 g         5507 t
ORIGIN      
        1 aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc
       61 tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca
      121 caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat
      181 gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg
      241 cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg
      301 ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt
      361 ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat
      421 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
      481 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
      541 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
      601 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
      661 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
      721 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
      781 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
      841 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
      901 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
      961 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
     1021 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
     1081 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
     1141 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
     1201 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
     1261 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
     1321 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
     1381 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
     1441 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
     1501 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
     1561 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
     1621 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
     1681 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
     1741 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
     1801 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
     1861 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
     1921 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
     1981 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
     2041 tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa
     2101 aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca
     2161 gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc
     2221 atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt tggttcttct
     2281 tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga
     2341 tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc
     2401 gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact
     2461 tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact
     2521 ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa
     2581 acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc
     2641 aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg
     2701 tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg
     2761 agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt
     2821 tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt
     2881 ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc
     2941 attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca
     3001 tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa
     3061 acttttttag catctaaacg gcgttttgaa agtaggtagc tgccgttttc tttatctttg
     3121 ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata
     3181 tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct
     3241 tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg
     3301 ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga
     3361 ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg
     3421 tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt
     3481 gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca
     3541 aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac
     3601 gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc
     3661 tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct
     3721 ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg
     3781 cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc
     3841 ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag
     3901 aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga
     3961 atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa
     4021 ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc
     4081 gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa
     4141 cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat
     4201 aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt
     4261 cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat
     4321 cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg
     4381 ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat
     4441 cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc
     4501 tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt
     4561 ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg
     4621 atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc
     4681 agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa
     4741 gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat
     4801 ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat
     4861 gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag
     4921 gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa
     4981 aatcgtttcc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc
     5041 tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa
     5101 ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg
     5161 caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt
     5221 ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctt
     5281 tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa
     5341 gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg
     5401 atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata
     5461 atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat
     5521 gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc
     5581 aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc
     5641 gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg
     5701 ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt
     5761 gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc
     5821 tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg
     5881 gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg
     5941 agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg
     6001 attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac
     6061 gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg
     6121 tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca cgatgatgac
     6181 cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg
     6241 caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg
     6301 acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt
     6361 cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta
     6421 cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga
     6481 ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa
     6541 taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg
     6601 tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta
     6661 ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca
     6721 aaaggtggat acatctgctt tgttgcagat aaacctctga ctcatcattg ttttttgcca
     6781 gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg
     6841 ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca
     6901 aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag
     6961 gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa
     7021 cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca
     7081 aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg
     7141 cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa
     7201 acttatcctg cttcaattct ggaaaactgt tatggataaa caaaagttcg tgggtaaatc
     7261 cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc
     7321 gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa
     7381 gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg
     7441 cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt
     7501 atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt
     7561 aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat
     7621 ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct
     7681 gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt
     7741 aattgactag ttttttcacg gaccctatag gacccattta tattatttca actttttcag
     7801 ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc
     7861 tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga
     7921 tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa
     7981 tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc
     8041 ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt
     8101 cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc
     8161 ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc
     8221 aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat
     8281 actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc
     8341 atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac
     8401 caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc
     8461 tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac
     8521 tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt
     8581 aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca
     8641 tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct
     8701 tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg
     8761 tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta
     8821 cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga
     8881 tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg
     8941 aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact
     9001 aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct
     9061 ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt
     9121 ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt
     9181 gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct
     9241 tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct
     9301 tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag
     9361 taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc
     9421 gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat
     9481 tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa
     9541 caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc
     9601 acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca
     9661 tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt
     9721 tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat
     9781 tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta
     9841 aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg
     9901 aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg
     9961 ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta
    10021 tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca
    10081 aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa
    10141 gaaaaaacac tcactgaatg gagctattga cttgtccacg tgcttatcga tagccgttta
    10201 tcggtttctt cattcttatg atagaccttg tacctgtatt tgtctaggtt tagaacattg
    10261 ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa
    10321 atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag
    10381 atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat
    10441 ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta
    10501 agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga
    10561 tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg
    10621 tgattaatcc attgttcata accacttgga taccacgagc aacagctact acgattgcta
    10681 caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc
    10741 cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt
    10801 accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg
    10861 ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa
    10921 tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt
    10981 ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt
    11041 tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat
    11101 acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac
    11161 cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg
    11221 ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct actactgtat
    11281 caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg
    11341 ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt
    11401 tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt
    11461 tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag
    11521 caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca
    11581 tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga
    11641 tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga
    11701 ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt
    11761 ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa
    11821 tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt gtaaaacttt
    11881 tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt aaagctttct
    11941 tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct tttaaaagga
    12001 gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa aaaaatactc
    12061 aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc caaaaaaaaa
    12121 gaccaagaaa tttcttgatc tttttgatat ttatttgact gctgtatgac gttgttcgaa
    12181 ctgatgttgt tttcgactta accatggaag aagttgcgca tgcagcacta aaaagacagc
    12241 actcacgatg aatcctttga tcaagttgaa aggaacgatc ccaatcagta catagtttgc
    12301 taaagacatg cccagcatct gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt
    12361 aataacgaaa taatttgcga tactcataaa aatagttaga gcgatggttc ctgtaatgac
    12421 acctaaaaat ttgtttcttc ctttatgctg tttctttaca ctaaagaaat aataaatcgg
    12481 gaatgtaaag atcgttgttg ataagaaact agcagtgtct cctaccaaat tatccggaga
    12541 aaaacctgta gtcagcagat gtaatactga acgcaagaac gctgttgcaa ctccggccgc
    12601 tggaccgaat aagaacatgc tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa
    12661 actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa
    12721 catcgctact gcaaccattt tctgtacacg ggtgttcttc atttgaaatc ctcctgttag
    12781 gactcctctt cagcgaactt tttgacgttt ctctccaaat gaaaaaactc tattttccag
    12841 ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa taagctctat cttctttatc
    12901 cagactatac tgtcggtatc ggaatttcac cgattcagct atttttttgc aaaataggtc
    12961 gtggactata accaccggtc gggaatttca ccctgcccct gaagacgaac cttatataat
    13021 ttttgtttac atattcatta tacatatcta ttcaaaaaac gcaacatcta acacttataa
    13081 aaagtaagtg ttcattttga gtggttcagc aaactgctga cttctttttt gttcagttca
    13141 cggtaatccc caggacgtag gccttgtaaa gtcagatctc cgtatttttc acgttttagt
    13201 ttttgcacag gaagaccaac tgcttgaagc atatttttca cttgatgatt acgtccttcg
    13261 tggatcgtca attccacgat actgtggttt gtcttaggat ccactgataa aatttggaat
    13321 ttagctggag atgtttttcg tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc
    13381 gattttgtag cgatcccttt tacctttgca acgtaaactt tatccacttc atgttttgga
    13441 tgtgtcaggc gctgagcaaa atctccatca tttgtcagta gcaagatccc cgaagtatcg
    13501 taatccagtc gacctacagg atagatacgt tcttttacca tcggcaaaag atccgtaaca
    13561 acttttcttc ctttgtcatc tgcaacagca gaaataactc ctttaggttt gtacaataaa
    13621 tagtaaccgt attcttcttg ataaattggg acttcatcca cttccacagt gtcatgtttc
    13681 ccaactttta tccctaattc ccttacaact tgtccgttta cttttacacg tcctgcaagg
    13741 atgaattctt ctgcttttcg acgagaagtg atgccagcgt gggcaattac tttctgcaat
    13801 ctctccatta caattattct cctttattca ttccatcaaa gaatagatct ggtgacacgt
    13861 cttcttcaag tgaaatttcc atttcttgtg tatcaggcag ttcttctaat gatttcaaac
    13921 caaaataatc taagaagtag ccagttgttc catataatat cggccgacct ggtccttcta
    13981 ctctaccttt ttcttcaatc agtttatggg cagctagacg ctgtaccgct cctgaaacct
    14041 gaactcctcg aatctgctcg atttcagctc ttgtgattgg ctgtttataa gcgatgattg
    14101 ctaaggtttc gacggcggct tgagataatg tgtttgatgt cccttgcgca tatcgcttaa
    14161 gtaagggagc cagctctttt ttagtcgtta atacataatg gctttccgtt tcaaaaatat
    14221 gcaaagctga gtgcgagttt tgagtgtaag attcttttaa ttctttcact aaacttgtga
    14281 cttctgcttc gctgagattt aacagtccgc ttaattcttc attactgatc ccttcctcgc
    14341 caacaataaa aagaagggct tcaagttcac ttaatttcgt catgttcggt atctcctact
    14401 gctggataaa gcataattga ttcatagttt tctttttgtt gcgcaatgat ctgccctttt
    14461 ttcatcaatt ctagcaaagc cataaaagta gtgacaactt cacttttaga aggtgactca
    14521 aaaaaagaat cgaaagtaca gccatgcgca tttcttttta tacgctcttc gataaatacc
    14581 attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat
    14641 ttcttacgtt ctaagatttt ctgcatggca cggatcaaat cgattttttt gacttgtcct
    14701 cgcttcaacg gcttttcagc atccgctaac ccatcgatgt tcataggttc ttttgtgtaa
    14761 taaagacttc gttcctctgc tttttctgtc agtctttcag ctgcatattt atatttacgg
    14821 tattctaaaa gctgagtgac caattcttct cgaggatctt cttcccataa ttcttcatcg
    14881 tctgtgccga attcttggac aggaagcaat gtcttgcttt tgattgccat caatgtagca
    14941 gccatcacta agtattctcc tgcaacagct agctcgagtg ttttcattgt atggatataa
    15001 ttcatatatt gctccgtgat cgtagcgatc ggtatatcat atatatcgat ttccatttgc
    15061 tgaatcagat gaagcagcag atcgagagga ccctcgaata catctaattt aatattgatc
    15121 tccgtcaact cattcactct ttcttctttt tctttgctag ccattttcca atgtaaggct
    15181 ctgtgatttc agttgcacgg atggagacat ccgggtactt ttcctgtaat tcatctaaca
    15241 tttgaaagcc catattctca ccgcgataag acggattcaa agaaatgtca tggatgatca
    15301 gctgatcgtt cgttgcccag taaataccaa taatcccttg aatgttggtt gaaccttctg
    15361 gcgtaaagaa aaaaagttca taatcgtctt cagattcgta cgtatcaatc tccttcaaca
    15421 aagaagtttt acctgtcagt ttcttatgga aactcaatag tcccatagca attttacggt
    15481 tttctttccg gtaatgtgtt aacatcttct caccttccta tgactaagcc cttggaaaat
    15541 gctgcttata aacatccgtc attcttttct tcgtgatatg tgtataaatc tgagtcgtag
    15601 agatatccgc gtgacctaac aattcctgta ctgtccgcaa atcagctcca ttttctagca
    15661 gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac ttctttgtaa atcccagcct
    15721 ctcgtactaa ctgcttcaga tttttccaga ttccttgccg agaaagacct ttcccatgat
    15781 tattgacaaa tacatgcatt tcagatgcgt cctttacaag taatggacgt gcttcgtcta
    15841 agtagcgttc cagccattgg atcgcataat cacctaaagg aatgatccgc tctttatctc
    15901 cttttccaac tgtctgtacc agtccaagag aaagatgaag atctcccaat ttcaatccaa
    15961 tcaattcact gacgcgcatc ccagtagcat acatgacttc caagattgca cgatctctta
    16021 tccctaacgt ttttgtcgta tcaggtgctt caatcagctt ttctacttct gtcagagtca
    16081 atgtacttgg taatttctgt gctttcttcg gcgtatcgat atgttgcatc ggatcatgat
    16141 cagtgatacg ctcttgccgt aaaaactgat ggaatcttct gagacttgag atcatccgag
    16201 taacagttgc tgtcgctttt ttttcttcat gcaacttctc cagaaaacgg atgactaaaa
    16261 agcgatcgac atcctgccat gaagcaattt tctgctcctg tagaaaatgc aagtactgtt
    16321 ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa tccccgttca atattcaagt
    16381 agtggagata atcgttgatt tgctctttca taataatccc cctcatgcct ctctatcata
    16441 tcagataccg cttcattctg atagtctttt tccataatct gagcaaagaa aaagccgtct
    16501 agacactcag acggactggt ttctttt
//
LOCUS       DAFFSV010000056        16281 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFFSV010000056 DAFFSV010000000
VERSION     DAFFSV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.056"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(63..560)
                     /locus_tag="LR110_001633"
     CDS             complement(63..560)
                     /locus_tag="LR110_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBL4822597.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(690..1400)
                     /gene="budA"
                     /locus_tag="LR110_001634"
     CDS             complement(690..1400)
                     /gene="budA"
                     /locus_tag="LR110_001634"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBL4822598.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1413..3077)
                     /gene="alsS"
                     /locus_tag="LR110_001635"
     CDS             complement(1413..3077)
                     /gene="alsS"
                     /locus_tag="LR110_001635"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBL4822599.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3282..3944)
                     /locus_tag="LR110_001636"
     CDS             complement(3282..3944)
                     /locus_tag="LR110_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL4822600.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3954..4769)
                     /locus_tag="LR110_001637"
     CDS             complement(3954..4769)
                     /locus_tag="LR110_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4822601.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5031..5474)
                     /locus_tag="LR110_001638"
     CDS             complement(5031..5474)
                     /locus_tag="LR110_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBL4822602.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5608..5946)
                     /locus_tag="LR110_001639"
     CDS             complement(5608..5946)
                     /locus_tag="LR110_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL4822603.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5934..6311)
                     /locus_tag="LR110_001640"
     CDS             complement(5934..6311)
                     /locus_tag="LR110_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL4822604.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6535..7728
                     /locus_tag="LR110_001641"
     CDS             6535..7728
                     /locus_tag="LR110_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4822605.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7894..8316)
                     /locus_tag="LR110_001642"
     CDS             complement(7894..8316)
                     /locus_tag="LR110_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4822606.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8711..8905
                     /locus_tag="LR110_001643"
     CDS             8711..8905
                     /locus_tag="LR110_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822607.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            8902..9396
                     /locus_tag="LR110_001644"
     CDS             8902..9396
                     /locus_tag="LR110_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822608.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9558..10730)
                     /locus_tag="LR110_001645"
     CDS             complement(9558..10730)
                     /locus_tag="LR110_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL4822609.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10936..11751)
                     /locus_tag="LR110_001646"
     CDS             complement(10936..11751)
                     /locus_tag="LR110_001646"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL4822610.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11901..12347)
                     /locus_tag="LR110_001647"
     CDS             complement(11901..12347)
                     /locus_tag="LR110_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL4822611.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12344..13348)
                     /locus_tag="LR110_001648"
     CDS             complement(12344..13348)
                     /locus_tag="LR110_001648"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL4822612.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13388..13717)
                     /locus_tag="LR110_001649"
     CDS             complement(13388..13717)
                     /locus_tag="LR110_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL4822613.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(13843..16170)
                     /locus_tag="LR110_001650"
     CDS             complement(13843..16170)
                     /locus_tag="LR110_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL4822614.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4642 a         3254 c         2879 g         5506 t
ORIGIN      
        1 ccactgtctt tccggctctt tttattcttg atttctcttc ttttttgttt caatagacga
       61 ttttagttat taataaatga aaaagcattt atattaaaat ttgctaataa aagatctaca
      121 tatttttcta gaaatgcttg atcgatttca tcataccctt tcgttactcc gcaatcgata
      181 tctaaaacac cgattaaatt accttctttt accattggca ctacgatttc tgattgtgcg
      241 gctgaatcgc aggaaatgta atttgcatgg gttttgacat catcaacgat caatgtttct
      301 tgtttttcag ctgattctcc acaaactcct tttcccatct tgatacgtgt gcaagaaact
      361 cgcccttgga acggtcctaa aatcaattca ttaccatcga ataaataata tcctgcaaag
      421 acagtctcag gcaaagtctc attcaacaat gcagatgaat ttgctaaatt tgctaaccag
      481 ttatcttctg cttctaataa tccttgttgc tgtaataata aaatttcgta tgcatctttt
      541 ttctgttttt ctgtccacat gataaaaacc gctccttttt gatctatgga gaagattata
      601 gctcagtttt tttgtaaacg ctaggtttcc tcctcaaaaa aacgagatac gtcaggttaa
      661 tcaccttgaa cgcatctcgc tgtctttttc tattctgctt gttggatttc tgctgctaaa
      721 tcagcataat caatcgtttc gtgaacaaaa gtcggatcat caactggaaa atgctgtaca
      781 agggacgaga tgcggctaat ctcaactttt ccttcagcca cttcaaaatc cattacatgt
      841 ccgccaaatg tatgtgtatc atcgataaaa tgcaagtgaa atcccgctgc agcaactcct
      901 tggaataatt caggcgtaaa gaacccgact aatgttcctt ggacattgct ttgttggaat
      961 tctggttgag actctgaaat ttttgctagt cttctgtaag gtttttcttg cttcggcatg
     1021 atacggatgt gcatattttt gaataatcca gttatcttca ctgccaaaaa taggttttcc
     1081 ccggcttctc ttactaatat gtcttttttc aattgttctc catctgcagt ttttgagaca
     1141 gaaaaactag tatctggcgt aaaatctgta attgctgcat agggggtaag ttcacttcca
     1201 tctaattggg caactgagcc atctgatctt ccttgataag ctacgccatc taaaaagatg
     1261 acttcaccat caagcccatg caatgttccc aatcccagag ttcctttttc taatatatga
     1321 gcaatcgttt ctgttccatc catcaagcca gccatcaaag ctcctaatgt cccgtgctgg
     1381 tataagattt tttcagtcat gtcgacactt ccttaataga attgatctgg taagattgtt
     1441 tctcccaatt tctcattatc acggtaatca atcggaatat caataattac gggtccttct
     1501 gtcgcaaatc cttcttctaa agctttttct aattcctcta cacttgtagc tcggattcct
     1561 ttagcgccaa aagcttctgc atatttcaca aaatcaactg gaccaaagtc tacacctgac
     1621 gcccgctgat atttcatttt ttcttggaac tctaccatat tgtagtgtcc atcattccaa
     1681 atcaaatgaa cgatattcaa ttttttacgt accgccgttt ctaaatcctg ggcagagaat
     1741 aaaaatcccc catcgccgga aactgatacg atttgtgtat ttggacgcac caatgcagca
     1801 gaaatcgccc atggcaatgc aacaccaagc gtttgcatac cattactgaa taacaaatga
     1861 cgaggttcat aacttctaaa gtggcgtgcc atccaaatat aatgactacc tacatctacg
     1921 gtaacagtca tatcatctgt tacttttgac tgaagcgtgt taataacttc taaaggatgc
     1981 aagatacctg cttctccttt tgattgaaca atgtcccgct ccgttaattc tgcttgaaga
     2041 gatgctaaat attcttttgc atcttctgac acttgttgag gctctaatga acctgtcaat
     2101 aaatcaagag tggctgagat atcaccgatc aattctcttt caggctgcat aaaaggatcg
     2161 atttcagctg gcgcttcatc gatcacaatg atccgcgcat ctttttccgc attccagttt
     2221 cttgcttcat attcgattgg atcatagcca atagcaataa ctagatcact tcgtttcaaa
     2281 agcatatctc ctggttggtt tctaaataaa cccacacgac caaagaaatg cgcttctaat
     2341 tcacgtgaga tcactccagc tgcttggaat gtctctacaa caggcaatgc tgtctttcca
     2401 actaattgcc tgatggctaa cgtttctttt tcacttgatc ctcgcatacc tactaatagt
     2461 accggtagtt ttgcttctct aatgcgttca gctaggtaac ggatatcatc tgcagaagca
     2521 gaacctaatt taggatcgct caatggcttg atgacattcc cttggacagg tgcatccact
     2581 acatcttgcg ggatactgat aaagctcgcg cctttttttg aactcttagc catacgataa
     2641 gcattggcaa tcacttctga aattgtttct ggatcttgga tttctgcact gtattttgtg
     2701 attggctgga acagtgctgc attatccatg ctttgatggg tcaacttcaa tagatcgctt
     2761 cgttttactt gtccggcaat tgctaaaact gggtcacctt ctgctgtcgc tgttaccaaa
     2821 cctgtagcta agttactagc accaggacca cttgttgcga taactactcc tggctctcct
     2881 gtaatacgtc cgattgcttg tgccataaat gcagcatttt gttcgtgacg agtcacgatt
     2941 aattcaggac cttgatcctc taattcatta aatacaccat cgatttttgc acctggtata
     3001 ccgaatacat aatcaacatg atgattaatc aaactttcta ctacagattt cgaaccttgt
     3061 ttcatttttt cgtccattga acatcgtcct ttttatgctt tatttttaat tattagttag
     3121 aaataatcac aaataattga tacagtttct aaacctgttt cattcaaaag tatagcacgt
     3181 tttatttcat ttgtttagcc atatgtttca aaaaatgaaa aagaatgaca gtttttacat
     3241 aaaaactgtc attcttaatt gaaagtttca atttataaag tctacagatc catagttctc
     3301 atcaatttac gattgttgaa gtaaatcaaa ccaaatacga tagaaggacc tagtagaata
     3361 caccattggg gctttacgaa aaagaagaga aacaaacttg tcaatagtaa aaaatctagt
     3421 aaactcacat accatttttt caacgtatag tagaacgcta tccgtagaac ttgatttggt
     3481 ttcgacgctg gatttctcac ctgaaaatac cagtagttga tgctaaagga aactgataaa
     3541 caagcaagta aaacaaacaa cggaatcaac cattttccat aagaaaaacg tataaaaaag
     3601 aaacaatcaa aaatcattat cacactaact atccaagcaa atagccaata aaaaaatccc
     3661 tttagccaaa attggcgaaa cgtctttatg aacaaacgta ctggtgaaaa acttttttct
     3721 tttgctaatt cttctgtcat acctatcatc acgatgcaac ctggaccaat aaaaagtaaa
     3781 gaaaaaataa aggaaaagac attccttcca tctatcgaca aaaggaacgc agctgctata
     3841 tacaagatat tgacacacca aaaacaaagg ctacttgcca atgattgata caaccaataa
     3901 aagaattcca tataaacatt tttgtcaaaa cgctgctctt tcataatcac ctcctattcc
     3961 ttcattcctg atgaagcgat cccttctaca aaatattttt gcatcgctaa aaatataaaa
     4021 gcaacaggaa gaacagaaac aactgaggca gccatgatta atgcgtactc tgcatcaaat
     4081 agcccaataa acattttcaa ccctaactgg attgttttat tttccgtaga agacaaataa
     4141 atgaatggcc ccatataatc gttccatgta ttaacgaatg tgatgatcgt caaactggca
     4201 atcgccggtt ttgtaagagg taaaatgatc ttgcgataga ttccccactc tgataagcca
     4261 tcgatccgcg cactctcaca taaagaatct gggatactac tgtagtattg cttcaacaag
     4321 aacacaccaa aagcattgaa cgcttgtaat agcaccaacg accacaacgt atatgttaaa
     4381 cccacttttc tcatcataat gaactgcggg atcatataag actgccaagg gacagcaatc
     4441 gtgccgatat atgctaaaaa taaaatatcg cgccctttaa aatttagttt agaaaacccg
     4501 taagcagcaa aactagaagt aaacaactga atcaacgtga tcacaacgct taaaaaagct
     4561 gtattcttaa aaaatgtcac taaaggaata cgagaccaaa taaccagata attctctaaa
     4621 tgccaagttt tagggatcca ttgaatcgga atcgtgaaca cttcattatt tgctttgaat
     4681 gaagcagaaa ccatccataa aaaaggaatg atcgtcaata aagctaatac aatcagcaga
     4741 atcataccca accattgaat aaagggcatg ttttttgaac gaatttttgc tttttttctt
     4801 tcatttcgaa cacctccaaa gttataatct tcatttcttt gtaatgatta caatccagaa
     4861 caacggttat tatagctgat tcatttccgt tcttcttatg tttgtaagcg cttatttaac
     4921 aagttatgta aatctcttta ttttttttga tttaaaaatg aaaaaataca aaagcaagac
     4981 tgtcatacgt ctcgcttttg tattgaaatt tcttcgatta gagatactta ttactgttca
     5041 aaagcttctt tgaaacggtg aatcaattca ttgactacag gtgataaaat ccgattcttt
     5101 ttccagacta gcatgcagtt cgtttcaatc attgggtcaa gcggaacgaa tgtcactcgc
     5161 tttgaaagat gacttacagc accttctatg actaaagcag cacctacctg attctcaatt
     5221 agcgcaaata cattgaaaat caaattgtac gttcctgtga tcagcaactg ctcctttttt
     5281 atgcctaacc agtcagcaaa cattcgctgt atttctggac gctttgagat caaaagaggt
     5341 acattcgcaa agtcttcttt tgttatcacg tctttttttg ctaaaacaga attttctaaa
     5401 atgagcaatc cccatttttc ctttcttggt aacaaaatcg tttcgtattt ctctgtatct
     5461 actggatcta gcaaaatcgc taaatctaac aggctttttt ctagtcactc cgtgatatca
     5521 tcacttgttc cgctgtggat atgaaattga acgctgggat aatcacggat caacttttct
     5581 agtaatagtg ccaatgtatt cgaattatca gcttccacac agccgatcgt aaatcggccg
     5641 ctgaataatt gtttttttct gttctcgaat tcttgagtgg tttgattcgt taaatctaaa
     5701 atttcttctg ctcggctttt ttagaaaata acctgcttct gtcaagatca ttttgcggtt
     5761 ttcgcgtaca aacagttctg ttcctagttc ctcttctaac tccttcagtt gtcggcttaa
     5821 ggttggttgt gtaagatgta gaacttctgc ggcttgagag attgttcctt cttctgcaat
     5881 tgtccaaaaa tattttagta agcgtaactc catagcgatg ctccttgatt tttttacttg
     5941 atacattcca ttttatttct agataacaca cgatcatatt tcttgatttt tccttgtaaa
     6001 cgttccaaac actcttgcat ttcagcaatt tctttcgtca aaagttcact ttcttcaatc
     6061 aaaagcttac gccgctcaaa tacagttgct tctccttcgc tagataggcg aatatattca
     6121 atcaaagact cgatcgataa tccggcattt cgcatacaga tcgtaaagtc gacccatttt
     6181 aggtcttctt ctgtataatc acggattccg ccttttgtcc gattaactgg tggaatcaac
     6241 ccgattcgtt cataatagcg caatgtatcg gccgaaatgc ccttttcttc actgacttgt
     6301 ttgatattca tctgatcact ctttctctta atttcttgcc actttactta tgtattttat
     6361 ttattctaca actttaagtg tactccaagt caatactcat ttctattaaa actaagctgg
     6421 attatctata tcggttgttt gtgtattatt tcaaataaaa aagtcttgtt tttttattct
     6481 tttttggata tacttttaac aatttaggga aattttagga aaaggggtat aaaaatgaca
     6541 acgtatcagc gaaaggtatt attatcgaca tcggcgggaa ttgctttaga gaatatggat
     6601 attatgtttt tagctttttc attatcttca atgattactt cttttcatat tagtggtacg
     6661 caagcgggtt tcatttcaac gatcactaat ttggggatgc tcattggagg tatttttttt
     6721 ggcatcatag ctgataaata tggacgtgtg aaagtatttt ctcaaacggt ccttctgttt
     6781 tcgattgctt ctttattgat gtattttgct tcaaacatct acttggttta tctctttcgc
     6841 tttatcgcag gtgtaggagc gggaggagaa tacggggcgt gtatgtcctt gatttccgaa
     6901 agtttttcaa aaaaacaaat tggacgagct tcttcggttg ctggtattgg tgcgcaggtc
     6961 ggtgcggctt tggctgcgat tttagctgct gtgatcattc caacgtttgg ttggaagatg
     7021 ttgtatgtcg taggtgtact gccggtgttg atggtcttgt tcattcgtcg cggcttgact
     7081 gaaccaaagg aattcaaatc tgtacaatct gccgaaaaga aaaccaaact tagcagactg
     7141 tttgaatcaa aacatcttgc ttggcaaaca tgtggcctta gtatcatggt aactgtccaa
     7201 attgccggct attttgggtt aatgaattgg ctgccttcaa ttatgcaaaa tcgattaggg
     7261 ctttcggttt ctgggtcttc attgtggatg gtcagtacta ttttaggcat gtctttgggg
     7321 atgatgactt ttggcataat catggataaa ttggggccta aactagcctt ttctttgttt
     7381 ttaatttgtt catccttatc tgtctatcta cttgtgttag cccatagcca gtggagtttg
     7441 gtagcagccg ctgtgatagt cggctacttt atcaatggca tgtatggtgg atatggcgca
     7501 gtgattagta gtttgtatcc aacagaaatc cgcgcgacgg cgaataattt cattatgaac
     7561 cttggacgag ctgttggtgg tttttcttct atcgtcatcg gtttcttgat ggatcattat
     7621 agtttaactg ccgtaatctt gtttttgagc ggtatttacc tagtcagtct gctggtcttg
     7681 acaactctta ctggtgttac agcattaagg gaaagcttgc agcattgata gttgagtctg
     7741 taaatgacaa gaaagaggtg tggcaaaagc ttgcatctgc ggtaataaga ccagccaaat
     7801 caaaaacatg agacactgtt tccgtttggc tggtcgtatt acttgatgca gggcagcttt
     7861 ttcaactgct aatttccttc tatctggcgg cgactattct ttgacgtccg tcatttcaaa
     7921 gtactttact aataacttag cataacgaaa aactgtggat acactcgtat attgagcgtc
     7981 tgcataatct tccaaatttt tctcaggatg ttgatacatg aattcaatca aatgataatt
     8041 atatgatttt ctgtagaaat gaagataaat aatgtagatt gggaagttct cctgcttttc
     8101 catagaaaag cgccgattat tctcattctt caaaatgacc ttatcaggat atttttggaa
     8161 caattcttct aaatctgtta gataattcaa gatagactta cttgatacct tcaattcgtt
     8221 ttgtagttct tctattgaac accaacgttc tgtttcaagc agtaaatgaa tcattttaat
     8281 tttcttttga gaaacttttg ttaaaaaagt ctgcatattt tctcctcctg tatctacttt
     8341 actatactac atacaagaaa aaaaacggat acaaatatca tattgtcaca ccgtcaatag
     8401 tgcataaacc tgttattatt tctctttttt tcctaagtat tctattgata tgaatcattt
     8461 aatcgtttat tatataaatg tcaataatct aataataaaa taacgtacat attttattat
     8521 tgcatactta ataacagaac tatagcaaaa aatcccgtgt atcaacacgg gattagatta
     8581 agtttcctca atcactattc ataacacaat tattataaca agtaagtaaa caattgaaaa
     8641 ggacatttat tgaaaacggt tgttttttac ttaaggagga aatttagcga aaaaattagt
     8701 tgcattgttt ttggtcgcag gaagtttgtt gagtgggtgt ggtgctacta cgtctactac
     8761 tcaaagcaca agttcagatc aggcttccag cagcagtacc acgatctccg aaagtacggc
     8821 agattcatcg aaagaacaag aacaaattga aaagaaaaaa gaagagaaac aaaaagcgga
     8881 aaaagctgca ctagaaaaaa agtgacagaa gcggaatctg ctttgtcaca agctgaagca
     8941 aatcctacaa aagaaaacta tgcaactgct tcagcagcta tccaagcgct acctgaaaac
     9001 aaacaagagt tgacaagccg tcttgctgca attgattcca caatcaaagc aaaagaagca
     9061 gaagaagaac ggaaaaagca agaagaaatt gcacgacaac aagcagaaca agcgcaacaa
     9121 gaagcaatcg cacagcaaca agcagaacag gcgaggcaag aagcaatcgc acagcaacaa
     9181 gcagaacagg cgcgacagca atcagcagct gaacaggcct ctcagcaggc acaagccgca
     9241 gctcctgagc aaaataatga acaaactgtc tatgtcacac cgaccggttc aaaataccat
     9301 acacataaat gtggaaatgg tacgtatacc cctgccactt taagcgaagc tcaagctcgt
     9361 ggtttaaccc cttgctccaa atgttatgga aactaattca aaagttatac tataataata
     9421 aaattcttct gaaacataaa aaaatagaag atggcgttcc agatagtaaa ccaaaaataa
     9481 cagtttacac gtccatttcg tcagcttctc tcttttttca tttagtctgt tccctctgct
     9541 tttttggaca tcttcattca ttctttctca atcaaatagt acacctcttt ttcttttcgc
     9601 agtttatatc ttcctttttt caatttcatt gaaaactgtt tcgaatgttt atgttttaca
     9661 tggattttaa gcaggagctc ctcatgttgc aagcgatagt tcctgccttt taaatcagaa
     9721 aatctcacta gtccgtattc atcagaagct ttcacaaatg gaactttttc tctagtaata
     9781 tagtgtttgc cgttgagcgt ttttacagaa aagaccattc cttcgattgg tcgtccgttt
     9841 gtgttcttta cataaaaaga taaaggatag tttcgcttag cgatggccaa agaatctaaa
     9901 taagcgatga gtagccaaac taagccgatt gatccaagta gtataccgat aaaccaaata
     9961 aaaaatttcc attttttcca ttgatcaatt ccttggatcg ctttttttgc ctcttttgct
    10021 tgataaggaa tacgatgccc cgtcacaagc agtcgatgtg tattgaccat ataaggagta
    10081 caggtaagca aagtcacgag atctctgcct ttttggatac ccaaagaatc tacttcatca
    10141 ggtaagacca cttggatttt ttctacttga tacgccaatg tctttccatt gatctggatg
    10201 aaaaactggt ctcctttttt caacttaggc aaatctgtaa ataattttgc ttctgggagt
    10261 ccacgatgcc cagaaagaac ggcatgggtg tttttcccgc cagtcggata agaactttct
    10321 tccaataatg acgttccttt ttgcaaaaat atttccgttg tctgatcgaa tataggtaag
    10381 ctgacattga tttttgggat ttcgatgact ccaagcatat gttccataaa aaaggcatta
    10441 ctaggtaaat cttttaatgc ttttgcatca actgcttggt tgaaactgcc tatccctggt
    10501 gatacgtttt gctctgccaa ctgttggttt ttctttgtca ttttttcctg ctgttttttt
    10561 atttcctctg aattcttatt cgatgcttgc ttttgataat ggatgatcat ttgctgggca
    10621 agaaaatcat tcagtgaatc ttctacaaaa ggataagaaa agatcccgat cccaacgact
    10681 aaaatgattg ccatgaagat gtccagtatt cttattctgc ttttattcat catgtccacc
    10741 tattttctaa tatgtatatg aagaggttgt gaaatcagcg ccttgacctg agtattagcg
    10801 aagcaaacgg caaaatagtt cctcctattt tgccgtttgt tgagcgaata ccaagagacc
    10861 gaccacttgt cacaaccttt ctccccattt gaatcactat cgtttattta tcgtatgatt
    10921 tttgtttcca atagtttaag cttctgtttc ttgatggcgt tttttgaaat agatcactgc
    10981 tcctccaact aaagcagtac ctaccaagat gaatacgatg atccccatac caccagttga
    11041 aggcaagatt cctttaggtt ggttatcgat cgttgtttca tcagaagttg ttggatcatc
    11101 atctactgta aattcagtgt acgcagcttc attcttaggt aatgcgtagc cttctggtgc
    11161 ttttgtttct tgaaggcagt atgccccttg tttcagccct tcgattgaaa atttcccttc
    11221 tgaatcagaa atcaatttca cttcttccca gtcagaacct gtgacttcat ctgaccaagc
    11281 atatgggtat tctcctgagg caaggtttgc tatttttttc attaatttaa cagttggatc
    11341 ttttgctctt agaatagaaa attctgcatc ggctaatttt tcttgcgtgt tcacatcttg
    11401 tttgatgaag tggaaatcac cgtagttttg gacattttct acagtaatcg tgttatccgt
    11461 agttgtattt tcccgattga ctacaccgtt tggattccca agattggctt catctgcgcc
    11521 atggatgata ccggttgttt gttgcaattt tccgtttttt actataaaag tagtttcttt
    11581 ttgtggattg gttaattgat aaccatctgg tgcttttgtt tcaactaaag tataattgcc
    11641 gtctattaat ccattgacac tgatggatcc ttcagtatca gttgtgaatg tcaacgctga
    11701 ctcagcagga tcatagagag cgacaccagt gttttcatca aaaccggtca cgaattgatt
    11761 ctctgaattt ttgatctgga attgcgcccc tgctaactta tctacccctg aaaagttccc
    11821 agctttctgg attttgatat tgctttcttt gatgacattt ttagcatata ggtgaatcgt
    11881 gtctaaaggt ttgtcagggt ctagggtaag cttaacacga tatttggcga cgtttcgaca
    11941 ccgggctttg gtgtttctac aaagacgtat acagcatctt tcccatcaga tttacctgga
    12001 agagtcaggt tagctaatcc ttcaccagaa gtcgttacac ttgcgaaggg tgtagcgtct
    12061 gctggaactg ctgtgtcttt acttggatag tacgtagcag tgatgtcata tacgttgaat
    12121 tcaacgccgt tcaacggttc gctattcgga aatgtcaatt ccgttccgtt gtttatttga
    12181 ttttctggtg ctgaagtgaa tttcaattta tgcaaattaa ccgtaacatt ttctggtgta
    12241 ggtgatacgg ctgcagaaac ctctgcagtt cctaggccga gaacgaaagg aagcagcatg
    12301 atagcagttc ctaaaaacgt ccatattttt gttttgatgt ttttcatttt atttccctcc
    12361 tattgtttcc tttttgttgt tttttataaa accaagctgt ccctgttgaa aggactagca
    12421 ataaccctaa aattgagaaa cctccaagca tctcttcgct tgtttttgga aagcttcctc
    12481 catttttttc atttgatgga ccatttgtct gcatctgaac tggcgttttt ccattattcg
    12541 tcggatttgc ttttggttcg ttgatgattt ccaatggtat aggatgaagc ggatcataac
    12601 tgcctttttc aacttcaaag agaatggttg attgactcaa tatatatcct ttaggcggtt
    12661 taatctccac tagttcgtaa cttccaaaag acaatccatg gacagaaaat gttccatctt
    12721 tactggaagt aaaaatcgtt gctcttgaat catctttggc tacccaatga tttatcccgt
    12781 tttcttgcgc taagtattgg tcattttttt tgactagaaa ttgagcatct gctagttttt
    12841 gtttcgtttg ccgatccact ttgacaaatt gtttgcctcc tgtttggatg gcaagatgtg
    12901 tttctactgt ttgattgtca gtcgtcaaac gtacatcgtt atcaaatgtc gttttttcac
    12961 tgttttcttt caatgtcatt tggtaacgga tcgttaattt ttttccagca aacgaactaa
    13021 gatcttttcc agaatctgat aagacaaaac catgttttat tttttgtgtg tgaaatgtat
    13081 gaatcttgtc tccaccaatc aaaaaatcga tcgtttctgg ttctagccat aactgaggat
    13141 cagctgtatc tttgatcgta taggttccta tctcgttgat gttctcagga attgttacgg
    13201 tgatgagata agggacttta tccccatctg caaaatctgt ctgcttattc tctactgttt
    13261 ttgttaatgt gagtgcagca ggtatttttt gttcgctctt tggatacaag tggatggtcg
    13321 tcaatttatg attagatgaa tctgtcacag gaagcacaac gacaaatggt gctgattttt
    13381 cttttactta atctgaagtc tttatttccg taaatcggta aacagcgtca cgaccttgct
    13441 catctttgtc cgatgcacta aaagaagcaa tgccctcttc ctcgtctact gtttttgtca
    13501 cattttctgc cagtattttt tcactatcac ttttttgtgc tagttttctt tgtgcgtctt
    13561 ccacactact tccttctgac cgcagcttgt aaaattcatc cgagacatca tatatagcga
    13621 aagtaacgcc gttcaaccct tttactcctt gaaagttatc cgggatattt attccgtcgt
    13681 tttgactgat acttggcatc ttatcgcttt cgaacattcg attatgcaaa gtaaatgtaa
    13741 taggtgattc tctagctgac acagtcggag aacctataaa aaagaccagt aggataaaaa
    13801 agagaacagc aaatatatcc acacgtacat tttttgtttt tttcatcgct gttcacctgc
    13861 ttttttctta cgatggataa aataaaagaa gccagaaaaa ataaagatca gtcctaaaaa
    13921 gacaaagggc agtagaccta tcccacctgt cgaaggaagc aagagagtcg gattgatcgt
    13981 caacttagcg gtatctgttg cgataatcgt ataattattg tttacacttc cttcgcctaa
    14041 actaatcgtt actatttgtg atccaatatc tgtccggctg ttaactgtta gggtataatc
    14101 caatgattcg cctaagacaa caccggtaac gtggagattt ccaaccgaaa tcgagtgact
    14161 ttgggcatca taattaaagg cagcgtcatc gacagtaatc actgcgttag ctttatagat
    14221 ggtataagtt cctgatgtgt tggctatatc agtaaatgac aacttgggat ttgctgcttt
    14281 gactttgtct attccgctat tcgttagttt aattgtatag ctgccagcat taaccatatt
    14341 ttttgctgat tgtgcatctg ctgtgtaatc catactgcta agtgccactt gtactggagt
    14401 aagcgaacta gtgacaccct ctgctagctt gacatggatt gacgggacac ctgcttttcc
    14461 atcatatgtc ttactgccac tgagctctac cgcaccgctg atttgcttaa tcgtatagac
    14521 atcagatgtc tgcacgatag taaataaata gtttgttctg gcagctagtt tagctagtcc
    14581 gctttttgtc aattgccaag acgtataggt tccaatttca gtaggcgcag ttacttcaac
    14641 tcccttttca ttcatcgctt tgaaatcagt tggattccat gtgatttgat caatactgag
    14701 atcattgttt aaaagggatg ttgattctgt tttatcatta aaggcatatt taacgatcgg
    14761 tacaacagaa agaccatcat agtacttact gccatttgca ttgattgata ctgttgcatt
    14821 tgtcttataa tataggatga cttcagaaat ctgttttcca tttgcgtcct ttacgattac
    14881 atttccattt gtatctaaac tagctaaaac actcattgct gattggttct ttgtttctat
    14941 cgctgaaaca gctgtaaagg tatatccgtt gatagtaggc gtattagtcg tcaggtcttg
    15001 tgcagcactt ccgtaagtaa gattcattat tttagaagga gcaatcatct gtccaaaata
    15061 gtcttgatac ttaatagtga ttggtgtttg tttgcctgta taggtcaccg tatcgacgtt
    15121 actttctagg gctgtgaaag aaccgtttgt ttgtgcaaaa gaatagcccg ttactgctgt
    15181 tggagtgata accactttgt cacccacatg gattccagat aatgtagtcc cgtacgttcc
    15241 gatttttgct gctccaccat tgactgtata agaaaaagtt cctgtcctat tcgcattgac
    15301 ctgaaccgtt gctgcttgat aggttggaaa atttgaaaca tttcccttac catcaacgat
    15361 ctgcccattt ttgatcgagt accccgtgaa tggaatatca tttgctaact gtataccgat
    15421 tgaaaaagta ctcatacctc ctccggcagg aagccctccc catgttgagg gtgtaaaaga
    15481 ataactttta tccgcattgg gtgtaactga atcgatcgta tttccagcgg tagccacccc
    15541 catatagata ctttttggga atgtgatagt agtaccattt actgtggagc caataaacag
    15601 attatggata tctggacggt tatcaataaa agcgattgct ttggttacgc cttgtaacgc
    15661 agttttatcc gcactcatcg atccgttaac cgcaacaatg actccatctt tggcatatat
    15721 cactgtatat ccatctggta tggtatgtcc gttgaaattc gcagtggccg caacagtact
    15781 aaatgtctgg cttctacctg tttctgaata agctttctcc cctgctgctt ttgctgctgt
    15841 gatttctttt tctgtcggat caatcagttg agtcgaggat gtggttgagg ttgtctcgtt
    15901 gttgctagac tgattggtgc tttctatgga ctgtatttca gaatagggtg gactatttgt
    15961 ctcctctgtc gtttggttag caaaatttga cgtttctgta taaacgttta cataaatact
    16021 tgataagctc cctaagaaag taaaaatcaa gatgatcgaa atcataatgg gatgtaagta
    16081 ttttctttta gatacagctt tgagtctaga aacaaaatgt ttttgacata tacgttggat
    16141 tttattttga gttttcttaa tcattttcat ccctcctctc aataaaaaat catcaaataa
    16201 tcctttacac gttattctaa atttaatttt tattatttta ttgatgtata gaaagtttgg
    16261 ggcttttttt ctctttttat t
//
LOCUS       DAFFSV010000057        16266 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFFSV010000057 DAFFSV010000000
VERSION     DAFFSV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.057"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            158..1795
                     /locus_tag="LR110_001651"
     CDS             158..1795
                     /locus_tag="LR110_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822615.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1940..2680)
                     /gene="truA"
                     /locus_tag="LR110_001652"
     CDS             complement(1940..2680)
                     /gene="truA"
                     /locus_tag="LR110_001652"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL4822616.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2871..3506)
                     /locus_tag="LR110_001653"
     CDS             complement(2871..3506)
                     /locus_tag="LR110_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBL4822617.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3653..4894)
                     /gene="sstT"
                     /locus_tag="LR110_001654"
     CDS             complement(3653..4894)
                     /gene="sstT"
                     /locus_tag="LR110_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBL4822618.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5092..8121
                     /locus_tag="LR110_001655"
     CDS             5092..8121
                     /locus_tag="LR110_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBL4822619.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSALFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQKK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8181..8897)
                     /locus_tag="LR110_001656"
     CDS             complement(8181..8897)
                     /locus_tag="LR110_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBL4822620.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(9124..9666)
                     /locus_tag="LR110_001657"
     CDS             complement(9124..9666)
                     /locus_tag="LR110_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL4822621.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9671..9898)
                     /locus_tag="LR110_001658"
     CDS             complement(9671..9898)
                     /locus_tag="LR110_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL4822622.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9977..11857)
                     /locus_tag="LR110_001659"
     CDS             complement(9977..11857)
                     /locus_tag="LR110_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL4822623.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(12046..12660)
                     /locus_tag="LR110_001660"
     CDS             complement(12046..12660)
                     /locus_tag="LR110_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4822624.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12802..13362
                     /locus_tag="LR110_001661"
     CDS             12802..13362
                     /locus_tag="LR110_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4822625.1"
                     /translation="MKKTDRRVKKTEKALAEALSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13667..13981)
                     /locus_tag="LR110_001662"
                     /pseudo
     CDS             complement(13667..13981)
                     /locus_tag="LR110_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14235..15875)
                     /locus_tag="LR110_001663"
     CDS             complement(14235..15875)
                     /locus_tag="LR110_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822626.1"
                     /translation="MQVKKKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARV
                     KGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNV
                     CTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYY
                     AYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRL
                     IVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDL
                     TTFTVGIIGIISLICPLFFFKGFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMI
                     QFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITAIVLLLLAVSAATKANYEHKV
                     VTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQV
                     MDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIDNSEGLVSIEGTQGETIIEV
                     SSIYPPTMKLAFLISLCSYLFLLISFLIRKLQHIKIMI"
BASE COUNT         5142 a         3106 c         2543 g         5475 t
ORIGIN      
        1 acttattttc atagatttgt tgttctcctt ttttaattct attttcagta tactagaatt
       61 gtcttttttt aataagtttt gcgttaaatt tttcatagca ctagtatgat atactagaaa
      121 agaatcaatt tttattactt ttaaatagga gaaaaaaatg aaaaaaaaca aaaaccaaat
      181 aatagatatc atttttatgt tatttctaag ttttgcttac gtcatccctc tattcatgtc
      241 taagggaatc tatcatagtg tcaatcagga tacttacttt catttatctc gaatcatagg
      301 actagataat gtatggagtt ctccagtaaa cttcaataac ttcgatcatc atggaacaat
      361 gatgaatatt ttttatccat ggcttactct ttatccagct tttttatttt ataaaatgat
      421 gggaaatctt gttttaagtt ataatatcta ctattttttt atcacttttt taacaatggt
      481 cgtttcttat ttttctatga aacaaataaa aaacaatcgt tatatttcgc tgttattttc
      541 gattatttat acattttccg cctatcgtgc cattgacata ttccatcgtg cttcattggg
      601 agaagctgtc gctttaacat ttttgccttt gatactaatg gggtgttacg aaatttatat
      661 tagagattac caaaaatggt actggttgtc tattggcatg acgctagtag tgtatactca
      721 tttactttct gtcgctatgg tttctgtatt tattggagga acgttgtttc tttcttttta
      781 tttttgggac caaaaaatcg ctagactctt aagtctttta aaagctaccg tcctcacctt
      841 ttttttaagt gcgggattct taattccttt tattcaacaa tctagagcac aagaattgaa
      901 agtcccactg ggaaaagaat taagtggtat ggctcctagt gatatgctga cacatatttt
      961 aaataataac tataacaact atactattgg attattctta tttctcggat tgattggtgc
     1021 ttttattttt ataaaaaaat taactgctga tgatttattt attttctttc ttggtgtatt
     1081 tgttcttttt tgttcaacca atttatttcc ttggcaactt tttaaccata caccagttaa
     1141 gagtctacaa tttgtctggc ggttaaatgg tttttcttct ttatttatag cctatacgat
     1201 gagtatagtg atctattata ttttttctac taaaaataat tcatggaaaa taatggtctt
     1261 tacgtttctt gcactaatat tgcatctctc aggagtaagc aattctatcc atgctaacaa
     1321 agattcatta acacttatag cacctgaaga cgctgttgct atagctgaaa attacaatca
     1381 tactgattat gcaaacaaag agtcaatcta tcatcctgat atgattaata atgatcaata
     1441 tttactaggt aaccaggaaa taaatccaac aactcatttt atgagtaaca aacttacaat
     1501 tgaattagat aattcgaata ataaaaatac tgtcttgacg acacctattt atcgttataa
     1561 agggcaagtg gcttctatta atggcaaact agtacaaaca aaattatcta agtttggcac
     1621 aactgaatta actattcctc caggtataaa taaagtcgtt attacttatc agtacacaaa
     1681 actagcaatt gcttcaagat atttatctat cgttactttg attttatttt tactttatcg
     1741 atttactttt tctaaacaaa aacaaccgag aagagaagta caacattctc actagatttt
     1801 tatttaagaa agacttttta gctgtacaca taaacaggct aaaaagtctt tttttatatt
     1861 tctttaattt gcttcataca ttatttaaca tcgatattta tactgtgaaa catgcgtttc
     1921 atccattatc aaagtatttt tatcgataat aaacttcatc caaaaacaga ccttgtgcag
     1981 ggacggtttc tcctgcatgt tgccgcactt tgctttcaaa tatttcatcg attgtttcta
     2041 tcggcatggc acctgtgccg atttctagta atgtccccat gataatccgc accattttat
     2101 ataagaatcc gtctccgaca aaagtaaaat gaagcatttc tccctttcgt tcgatagata
     2161 attcatcaat cgtacgcact gtggattttt ttgatttttt caatgcagaa aaaccaatga
     2221 aatcatgtgt acccactaat ttttcgcaag ctgcattcag cttgtccata tccaactctt
     2281 gagggaaata aaagctgtgg tttcgctcaa atgctgaagg aatcacgttg ttccaaacat
     2341 aatagctgta ttttttgccc accgcattat atcttgaatg gaagcgctca ggcatttctt
     2401 cgattctttt gatgacaata tcccgcggaa ggtaacgatt cataaagtcg atcatttctg
     2461 ccagtccaat ttctgtgttt gttttaaaat ttgctacttg tcctcgcgca tgggtgccag
     2521 catctgttct tcctgatcca ataatttcta tctcttcatt tgtcatctga tgcaatactt
     2581 tttcgatttt tccttgaatg gttttatcgt catctccaag tctttgccat cctgaatatc
     2641 tttttccgtc atattcgatt gtcaacttga tgtttctcat attttagtgg ctcctctgtc
     2701 ttccatttgg tcttctctct acctttttaa gattgtagca caaaatatag ctccttgctt
     2761 ctctaagaca gaagctatac aagaaaactg tggaaatata tatttcagaa aaacaaaaaa
     2821 cagccaccgg atgaagtatc cgctgactgt tcaatttgac ggctcaagtt ttactgtttg
     2881 agatgcaaga tactcaacca ggcaatgggc acataattaa agaaaaaact gattctttgg
     2941 caaaaaccta ttttattcag taatggccaa gaatttatag aaggaattct tgccagtcca
     3001 taaatcgccg caaaaagcaa actgatcaca gacagtccaa ggtataagtg gcttacgtcc
     3061 atttgcccat tttttcgata gatcaagaaa agaagaactg gaaacaataa aaaaccactg
     3121 taacctaaac ctgatccgat attatggacc catgtcgaga cattccacga agattcatgg
     3181 gtattgatac tgaacaaacc tgtaaagata caatcaccta taccatagaa cgcgatggct
     3241 gctgtaacta gggtagctaa cacacgagat gtacttacaa atgcagcata caatgcaggc
     3301 aatgacagga tgaaaaagat ccctgaaacc acagaccaaa taagaaacgc ttttcttaca
     3361 ggactggaaa cctctccaaa tacactgatc acgctcttca tttgatctaa ttcaggataa
     3421 tacaatccta atacgtacgg agtcaaaaaa tcactcagca cgcctaataa taaaaagaaa
     3481 aaaccaaatt tacgtaaaaa atccatgata cgcctccaat aacaattacc tatgctttta
     3541 attgttatta taaacgactt tatcttgatt taacagaaac ctctatttac acgaacatct
     3601 atataactca ttaaaaactc gaatcatttt aaaaatacta aatatctaac gttcaacgtt
     3661 cacttaatcc atctaaacaa tctttcaatt tgattttttc cccacttctt ttacgcgcgc
     3721 ctagctctcc tattgcagta aacaacagat cacttgacga atttaaggca gtttccattg
     3781 aatcctggat aacaccgata ataaatccaa taccgacgat ttgcatcgcg acatcattag
     3841 agataccaaa caaactacat gctaatggaa tcaataacag tgaaccgccc gcaacacctg
     3901 aagctccaca agcagctaaa gcagacaaaa tacataaaag gactttcaaa acaaaaggtg
     3961 cttcaatccc taaagaatgt acagcagtca acgtcatcaa ggaaatggta atcgcggcac
     4021 cgcccatatt gatcgtagca cctaacggga tcgatatgga ataagactct tttgtcaatt
     4081 tcaatctttc agccaacatc atgtttatcg ggatatttgc ggcagaactt cgggtaaaga
     4141 aagcgggaag tgcgctttct ttcaaacaaa agaaaatcaa tggataggga ttttctttaa
     4201 taaacaaaaa cgtgaggaat gggtacacaa ctaatgcgac aaacagcatc gtaccgatca
     4261 aaacaagtaa aagctgcaga tactctgcca atccagcgat tccagtcgta gcaattgatt
     4321 gataaaccaa tcctaaaatc cctatcggag caaatgcaat aatcacttgt acgacttgtg
     4381 acaacgctat agatatctga tccaccaact cttttgtcga ttctgaacgt gctcgaaaag
     4441 ctagtcccaa caatacagcc cagaataaga cgccaaggta attcgcatca ataagagatt
     4501 gaacaggatt ttgtgtgaca ttcgtcaaca tagtagataa aacactttcc aaactttgcg
     4561 gtgctttttc agatactgtt tcttgtaaag taataggaac agtaaaaatt gaacttacaa
     4621 taactgctac aagtgcagaa agcaatgttc ccactaaata taagatcaaa ataggcttca
     4681 cgaatgtctt attcccaatt ttatgcttgg caattgacgc catcgtcaag aaaaaaacca
     4741 gaataggtgc gattgccttc aagcaactga caaacaacgt tcccaatatt gaaataaatg
     4801 tccatgtggg gaaaaagata ccaaacaata ttccgactaa aataccaaca acaatacgtt
     4861 gaactaacga catatccaaa aacttctcta acaaatgacc accctttcac aattttaaca
     4921 atcattgtat cattttatgc aaagaaatta aaggtataat tttctaaaaa ttctaacaat
     4981 acaaacaacc atttcttgct aattttgtac tttttctgtc tacacgttgt tttttgagat
     5041 attctaagtt aggatgttat taatgggcaa cttatcagga aacgttttct tatgtttttg
     5101 tccaattttt ttgtgaggtg tttattcttg aaaaaaataa agaaggagag ttatggcttc
     5161 ctgacaatct ttgtcgcttg tataatgatg gttttacagc gtatattgat ttcagcagtc
     5221 actattccta catatgcaac aaaccctggt cgattctaca tctatacatt tttacaaact
     5281 tccgttgtta ttggtgcaaa tttgatccca ctttacatgg gttaccaata tcaaaaaatc
     5341 gcccgtttaa aagttttaca ttatttaagt cgttttgcct ttatattcct aatagccaca
     5401 ctagtttgca atatcttttt ttacgtacaa gctggcagct tgaatattcg tgattattgg
     5461 cttatcctaa cacctatcag tcagaattac tttacttatg ctgtttcttg tactttgctt
     5521 ttttgtagta ttccttatac agttggattt ttaaataaat tatcgaaaac aacaattaaa
     5581 tatctactat tatttttaac aattggatta gttggctgct ctgctctatt taataaagac
     5641 ccgtttgcat tacagaatgg acagagtatt ttatggattt tttatttatt tttaatgggt
     5701 tatggtttga aagaatggaa ctggaaagaa aaggtccgct ataagcttcc tcaagtattt
     5761 atcagcttga ttgttttatt cggtttgatt attttgatga cacaaatttc actcatcatc
     5821 agaggaaata cagatacagc gctgaggttt tctaaaccat attctctctt cgcaatgtat
     5881 tatactttct cttcgttttt atttttagaa atgctatctg ataaattagg tataaaagga
     5941 agaggaaatt ttcttgctgt atttttgatt gtcactcaag ttatatgtaa tttcccttta
     6001 gtttcttatc gcgtatctac atttatgaaa cgtgaatttc caaactcagg gaaggcatgg
     6061 ctgataaata tcgggcagtt ttttatttgc tatttattag cagtgtccct cctagttatc
     6121 tttttatttt atatacaaaa aatccgtttc attcatcgtt tcatcgaata cttcgctttt
     6181 gattcgattg aacaattaac tcagcgttgg aaaaaaagaa ttcattggct ttttgttcat
     6241 aaaagttatt ttttagttgc agcatttttt tactgcttta ccttcgtgca gattttcgtt
     6301 ttagaaccaa aagaaggttg gaaagaaact atccaggttg ccttaaaaat atttactcag
     6361 cgacaagcac ccatgatcct gacaatcatc attattatgg gatttttctt cttactattg
     6421 ttgatttcca atcgtttttg gtacgccttg actgctacct tgatcattaa cttactgctc
     6481 actatttcga cagtaatcaa aatggaaatg cgggaagagc ctgtctttcc ttcagattta
     6541 aaaatgctga ccggattgtc agaactatta tcgatggtta gcccagtctt attaatcgtg
     6601 ggcggattga tccttctcct attattgatt actagtatta ttgtacaacg tagactacaa
     6661 aaacagtatt ccttgaagat tcattggaaa cgtcgattta tcagtatcgc tgtattatta
     6721 ggatgttttt cgggtgtctt tttcattaat cacaaaaatt cgccttcttt cttgctgttt
     6781 aatttattta aagtaaacaa gactttcttt aaccagcaag gcgccgtaaa agataacgga
     6841 ccagtcattc aattcctaaa caatatcgac attaaaatca tggaagaacc tgctggctat
     6901 tcgaaagaaa aaattaatgg aataatgaaa aagtacaaca caaaggcaga tgaaatcaac
     6961 caaacaagaa gtgactggtt aaacaatcag acgatcgttc tcaatttaag tgaaagcttc
     7021 agtgatcctg cacgtgtccc taatctgaca gtacctacag accctattcc ttacatcaaa
     7081 agcattataa cagaaggaac aggaggattg atgttatccg tcggttatgg tggcggaaca
     7141 gctaacatgg aatgggaagg actgacaggc ttgagtatca gtaacctgtc tgcttcgtta
     7201 gttacacctt atacccagct agtggagcgt cagaaaataa gtccaaatat taccgatcta
     7261 ttcgatgaaa aaatagccat ccatcctttt acagctgctc tttatcgcag aaaacaagtg
     7321 tttgataaat ttggttttga caggttctat tatttagaca gtccggataa attaacttat
     7381 acggataaaa tagaacaaag cccacgcata tcggatgatt cagcatacaa agaaaccttg
     7441 aaagctttac aaagcaatac agaaacgtca caattcatcc aattatccac tatgcaaaac
     7501 catatgcctt atgatgatta ctatagtgaa ttgaactaca ctgctgaagg aactgctgtc
     7561 attcctaata gaagaaaaga attacaaact tatatgcaag gccttcacta taccgataca
     7621 gcagtaaaaa aattcatcca agaaatcgac aaaatcgata aaccgattac ctttgttttt
     7681 tatggtgacc atttacccgc aatctatagt gggaatgatt cgaaaaaata tggattagaa
     7741 cagcaccaga cagattattt catttataac aacaagtata gtcgtgaaca agcagctacg
     7801 ttaaataaag aagttgttgc tcctaataac tttggtgcta tggctcttga gcatgcgaat
     7861 atcaaagtaa ctccttacta tgcgttgttg acagaagtat ctaatcatct acctgcatta
     7921 acaattgatc ctacagaaag cttgtctaat cgttttaatg gaaaacaggt ctttgttaca
     7981 gaggatagtc aaattatcca aaaaaaaaat ttaactgaga aacaaaaaga aattctagaa
     8041 gattatcgtc tgatacaata tgatcttgtt gcaggtaacc agtacagtgc aacatgggcg
     8101 acacaaaaaa taaaagacta acaaataaac tgagcaaggg gttgtgataa aagacatgtc
     8161 acaacccctt gtttatctca ttaatttgct tgtccataaa cgatttgatt aaagccattt
     8221 ttcaattcat tattcgctgt ctcgttgaag taaagataat tatcgggatt cccctgattt
     8281 tgttgtgtca ccaatacttt tggctgattt tgctgaaacc cgaatagata gacgtgcttt
     8341 ttcagatatg gttctgtctc tgcatcggaa tagacggctg tgatttgata gtctgcttga
     8401 cctgtcccac tttctgacca ttctgcagaa acaggcactc caccaatagc catcttccac
     8461 tccccgttca aaatagcttg agggacttta agaccatata agtccacact catttgattc
     8521 gtataacttt tgtattgttg tcccattgtt tggccccatt tggtcataaa tgcctctaat
     8581 tgtgcggatt tgtctgcatt ccacagtgtt tgttcaactt gttcgctgct ttctgaagag
     8641 gaagatgaag cctctacaga agaacttgaa gtagatgttt gagttgtttc agtcgttttg
     8701 gaagactctg acgaagtaga ggtatctgct gtttctgtac ttgctgcagt tgacttgctc
     8761 tctgtgctag ttgttgtagt agttgcaact gttgctttcg ctgtttcttt tgttgattct
     8821 tttttcgatt cattcccgtt ggtacaacca gctaacaaaa aagctagacc aattgttact
     8881 aaaatccttt ttttcaatta aaaaactcct ttcgattact cccacttctt tattctatca
     8941 aataagcaat gtgattttat tgatttttcg tattttctca tcaatctttc aaatcttcta
     9001 aaaactgaga agcacccgct gatgttgtct gtaatacatc agcgggtgct tcttttattt
     9061 taatcttcgt gattttccaa tttacctcac gctttatatt tgtatttgaa gaaacataga
     9121 cttttagtca tcatcgtctt cctcatccaa atcttgtcgg acagtttttc cttggagtct
     9181 ttgtacttgg tagatgattt ctttattgaa acttaaaagt tcaacaagca attgttgcaa
     9241 gccatatttt tcttcttttt cagctaattt gattgcccgt gtgataaaga cattttggaa
     9301 atcaaaatct tttgcaaagt tttccaacat tgttgaggct ggtaaatatt tgttttgacc
     9361 atgttcgtaa atttttccat aacgcatgac ttcttctaaa gtagacggaa cgatttcatt
     9421 ttcaatcact aactcatgag agattttatt gaaccatctc gcttcttgat cagcatattc
     9481 tgaaaaaagg gtacttaatt cttgcgaaga tgtacccaaa acatacaatt gtgcttggat
     9541 caatttcatt tcttctaatt taaaatttgc taatacatgc cccaccatgg cgccagcagt
     9601 tggtttatga tggtcgatat ctgcttgttt tgtttcttga acaaattttt cttccactat
     9661 attcattgat ttacgcctct tttacttttg ctgattggac ttcataacct aatttttcca
     9721 caactgcagc taattgttca gggtggactt catctggatc aaagtttgct ttgattttgc
     9781 tcgcattaaa caaaactttc gtattgctta ccccaggctg ttgagaaacc gcatgttcga
     9841 ttttttgcat acagcttgga caagctagtg tatctaattg cataataatt tttttcatcg
     9901 tagttcttcc ttcctttttc ttgatagtct tattctagat tacagcttcc ataaaaaaat
     9961 tgattcttat caagttttac gatttttctt ctttgctttc gtgtccaaaa tacaataaac
    10021 gcatcgcgtt aaagataacg atcaagatac tagcttcgtg aacgaacatc ccactagcca
    10081 tgtaaataaa cccggcaaat aagccgatga gtaaaaataa gacgacacca atggcaatca
    10141 ctacattttc acgggtattt aaaacagttt tcttagccaa gcggtaggca tgaaccaacg
    10201 attcaaaatt agattgcatc aagacaacgt cactcgtttc gatagcaaca tccgttcctg
    10261 atcccatagc aatcccgata tcagccgcag ccaaagaggg agaatcattg attccatctc
    10321 caataaaagc aacgcgcaat ccttgttctt ggtatttttt cacaaattga accttttcgt
    10381 ctggtaataa ttcagcatgg acttcatcaa tgcctaacat atcagctaca taatcagcag
    10441 tcatctgatt atcacctgtc agcatcacta actgttttgc tcctgcttgt ttcaacttag
    10501 ctaattgttc agcgacttct ggtcgaatca catctgacac acctatgatt tgagtgattt
    10561 ttttgtcgat tgctacaata atgaccgagc tgcctgtttg ttgtaattga tgtaaatctt
    10621 gtacttgctt tggtgtgaga gaaagtttgt gagcggcaat caatttggtg ttgcccgcac
    10681 aaactgtatg accatcaatc tcagctgtga ttccttgtcc cttgattgtc tgattatctt
    10741 tgactgctaa ttgttgaaaa tcaatgtctt tctcctgagc ataatggatc acggcgcgcc
    10801 ccagaggatg atcggataac gtttctaatc gagccactaa agctaagctg tccccttcat
    10861 cctctgtata gttcttgata gtggatacag cagtagttcc tttggtcaat gtaccagttt
    10921 tatcaaagac aaaagtatcg attttggaaa aagtatccat tgcttcaccg cctttgacta
    10981 agatgccccg tttcgctcca ttaccgattc ctgctacatt cgaaacaggc gcaccaatga
    11041 ctaaagcgcc aggacaacca agaaccaaaa cagtgattgc taaacgaaaa tcacgactaa
    11101 tcagaccaac aaccaacgca ataagtaaaa cggttggagt ataatactga gaaaatcgat
    11161 caatgaattt ttccgcatgg gatttggtat cttgtgcttc ttctactaat tcgataattt
    11221 tggcaaaggt cgtatcatct ccgaccttag ttgcttcaat cgtcacgtag ccatctgcta
    11281 aaatcgttcc agagaagact tcatctccac tgattttgac gacttgtcgt gattcaccag
    11341 tcactgcggc ttcatccgca taccctttcc cgtcaatgac tcgtccgtcc acaggaatag
    11401 ctccaccagt ttttactaag acgatatcgc cttcttccac gtcatcgatt tccacttctt
    11461 cagtcgttcc atcttcttgg acaacactag cagtagatgg ggccatctcg gtcaaagcct
    11521 tgattgactc ccgcgttttg gtcaatgttt tttgttccag atagctacca aacaaaaaca
    11581 agaatgtaac gatagctgac tccttgtatt ctccgatcat aaaggcacca agcacagcaa
    11641 tagtaacgag taaatcaatt gagataactt tgactttcac tgcctgatac gcatggatca
    11701 taataggtat gaccgcaata atagaagcta tggctagagc aatgttatag aacaagacaa
    11761 aaccagtaaa ttttccgata aaaccaaaaa taatcaacac agctgataag gcggtgatat
    11821 gatttcgatg ctcttggatc cacttttgta atttcatctt tctccctctt ttcctttcat
    11881 tcttacgaga ctaagtatag atacttatcc atcataaaaa attgaccact atcaagttta
    11941 gtcaaaaagg cggttttctt ttggtagaat acactacaaa aaatagttag caagaagact
    12001 cactaaaagt gatcttgcca actattgtcg tttctatcga aattttcatt gaatgatttt
    12061 cctgtacagc atacatcgga gatctatttc tttatctaag atttttatct tgctaatttt
    12121 ttcctttagt ctttcaaatc cacgtcgttc atagaattga tacgtacagc cactatctgt
    12181 aaaaaggaat atttcttttc ctttctctct tttttcaaat tctgttagca acttggtacc
    12241 gatgcctttt gttttcaaat caggatttgc agctaaaaaa atgatctctc catctggcga
    12301 atgcttcttt aaatatgctg caagcatttg ttcatttgct gtctcgtaag ctcctttact
    12361 ttctttagca aatatatttt gtaaaaaatc gaatatctta acatatattt gctgtccaaa
    12421 agaacgatgt ttcttttttt ctccttttat ctccgccagc aaaacacctg ccaactcgtc
    12481 ctctgcatac aaagcaagaa tttgagttgc tcgattcaac tccatataca aaaaatatct
    12541 tccgtaaaga ttcagcagaa gttcattttc catatatctc tcaaagttca ttccttcaac
    12601 agcaaaatag atagcttttt tgtaatcttt cttttctaag ggtttaaaag ttagtttcat
    12661 catttcgcct cctcaacttc aatgattgtg ttatacttac attataatag acacagtgtc
    12721 tactatctat aatcaatcat ctataagttt gtcgattaag cgacagcttt gctttgatat
    12781 gtcggataag gagataggta aatgaaaaag acagatagac gcgtcaaaaa aacagaaaaa
    12841 gctttggctg aagcactatc cacattatta gtaaataaaa agattcaagc aatcactata
    12901 cgcgaactaa cagaaacggc agatgtacat cgttcgactt tttatacaca ttataaggat
    12961 atctatgatc tatatgatca attagaaagc aacttttttc gggatctaaa tgaaattctc
    13021 tcttatgatc cagcccatag ctatgaggaa ctatacactc gcttgattga ctatgtttac
    13081 gcaaatactt ttttatatcg cttatttatc ctcaaccatg gtgatatttc gtttcagaag
    13141 aaagttaaac aagttattga agaaaactat ctgaacatat ggctatatga agacaagaaa
    13201 acgattatca cagaagaaat gagattcttt actacgtacc acgtccaagg ttgtctatcc
    13261 gttctcaatc ggtgggtaga aagcaattac aagtactcca aaaaagaatt attagaactc
    13321 ttacagaaac tgaatgatca catagagaaa atcatgcctt gaataaaaac atactaagct
    13381 tagttaagaa gaaccgtaac cttctcgtat aacaactgaa tcttatttag ccaaattgtt
    13441 tactgaagat tttttgagta aaatttctat aattcctact tattgaaaag tagtttgtag
    13501 tgctgcttct ccctctctaa gtgatatatc ttgttgtacc aatcaaattc acctcatcat
    13561 taataaatta cttttaggct aatttattac aacttatagg ttgaagctgt aaccaattta
    13621 tagtcatatt atatttttat catccagttt attgaattct tctcgtttac ctcaattcat
    13681 tttcaagggg aatcagctca tcatctaaca ttttacgcca tgctcgcttc atttcttgtg
    13741 ggtatttaaa tggtttcgct tctggatgtt tggctttata tccttcgtaa tccttactgt
    13801 tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc agattagaag
    13861 catgtaattt tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac
    13921 ttacttgtgt aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac
    13981 cttcatttct ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag
    14041 taagattcct ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc
    14101 ccctctttgc cattaaaaat agcccaattc tctataaaga agattaagct tcattattat
    14161 aatataaagt ttaaatttgg tttattactt actttttctt attctctagt aagctaatat
    14221 tgaaaaaaat gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta
    14281 attaaaagaa agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga
    14341 taaatggaag aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct
    14401 tcactattat caatctctct tttttgtcca tttacttttg cgactgttcc ccaataatca
    14461 aatacaggaa gcgttatttt ttgtccttta gattcatgta ctgtatagac tattttcgaa
    14521 tccatcactt gataaggcat agtatgccat tccttgccaa ttcttacttc atgattctgc
    14581 aaatgttctt tgtattgtaa tgcttccgtg ggtgcatagt cataaagtcc tccgggaagg
    14641 agtgtcgtgt aacgaataag attttcttga ttcattacca agtgatctct ctgagtgaca
    14701 actttatgtt catagtttgc ttttgttgca gcagatacag ccaacagtaa taaaacaatt
    14761 gccgttatat taactacttt tctttgctta gctgatgtgc tcttcatgaa aacgaacgat
    14821 aaaatcaacg agccaaacag tacttgaaag cctaagattc gccaaggaaa ttgaatcatt
    14881 tgaatgggtg tattttgtaa taaagaccat ggaaaaaatt tggtggataa gagccaagag
    14941 actaccgcac taaggtaaat cactttttct ttgcgattaa atcctttaaa aaagaagagt
    15001 ggacagatca aagaaataat accaatgatt cctaccgtaa aggttgtcag gtcattattc
    15061 atcgcaccaa tcaaactatc cgagacattt aatgcttggt gttgtaaggt cgttttatct
    15121 ggctcattaa tcgattgaac catgatttgt cggaacatcg gataccaaaa gtatgctgtt
    15181 aaaagaatcg tcattcctac tgcctttgac aactccacaa tcaaccggat gctttctttt
    15241 tttcttgttt cacttactac aaagccgcct aagactagta agccaacggc tattacactc
    15301 ataaaagtgg aaagaacatg tgtataaatg agtaagctca tactgactgc taatggaatt
    15361 gcacatggct ttttctcata aatcagtgcg taagcataat acataactat cggtataacc
    15421 gtaagtgcaa tcgcttcagc aatagcagaa cgatgataaa tatcaatcgt acggtaccca
    15481 taaaatgtat ataaacagct aaatataaaa gccgcatcga ttctttttaa aaattttagt
    15541 ccataatgaa acatcagcat gatagtacaa acattcaata gccaaacata taagatataa
    15601 ctaacgatta aattattact tatccaataa aaaatgactg ccggaaaaaa ggtcaagtag
    15661 ggataaaaat aattcactcc gctcccataa tgattaaacg tcgtaaaatt gattggacca
    15721 gaaaaaattg aagataaccc tttgactcta gcaaaatgga aggcaatatc ccccgatgta
    15781 gaaatagtat gaacatttct aaaataattg attgaataaa gtccagcaat aattgctagg
    15841 agaatggaca atattatttt tttctttact tgcattactt aattatatac tcctttcatg
    15901 gattgcttta tctagtataa cttaaatttt aaataaatcg ttatttattt ttactctcac
    15961 ttaaaaaagc actaaaatta taagagaggg aggtaatggt ctgaacacca agaattgttc
    16021 caacatcgaa ttgcttaact aatgagttca aagcttaatt atgtggctct tatatttacc
    16081 gagcgtggat tgcgatctct ttagcgaaac aaacatcttt cataaaaaaa acagccatat
    16141 ccgctttctt ggatatgact atttactact actttactat ctttcattct tcttgtctca
    16201 cttatgataa ggcgctgcgt atcatgagtg aatgatatta ggaaaacttc ataaaataaa
    16261 tctttt
//
LOCUS       DAFFSV010000058        16137 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFFSV010000058 DAFFSV010000000
VERSION     DAFFSV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.058"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..394
                     /locus_tag="LR110_001664"
     CDS             <1..394
                     /locus_tag="LR110_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL4822627.1"
                     /translation="STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSS
                     TSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQS
                     NNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(549..1535)
                     /locus_tag="LR110_001665"
     CDS             complement(549..1535)
                     /locus_tag="LR110_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL4822628.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1558..2124)
                     /locus_tag="LR110_001666"
     CDS             complement(1558..2124)
                     /locus_tag="LR110_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBL4822629.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2138..2554)
                     /locus_tag="LR110_001667"
     CDS             complement(2138..2554)
                     /locus_tag="LR110_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL4822630.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3011..4945)
                     /locus_tag="LR110_001668"
     CDS             complement(3011..4945)
                     /locus_tag="LR110_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL4822631.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5226..6182
                     /locus_tag="LR110_001669"
     CDS             5226..6182
                     /locus_tag="LR110_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBL4822632.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6272..7375
                     /locus_tag="LR110_001670"
     CDS             6272..7375
                     /locus_tag="LR110_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBL4822633.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7388..8341
                     /gene="menA"
                     /locus_tag="LR110_001671"
     CDS             7388..8341
                     /gene="menA"
                     /locus_tag="LR110_001671"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBL4822634.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9155..10501)
                     /gene="gor"
                     /locus_tag="LR110_001672"
     CDS             complement(9155..10501)
                     /gene="gor"
                     /locus_tag="LR110_001672"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBL4822635.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10635..11888)
                     /locus_tag="LR110_001673"
     CDS             complement(10635..11888)
                     /locus_tag="LR110_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL4822636.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11894..12817)
                     /locus_tag="LR110_001674"
     CDS             complement(11894..12817)
                     /locus_tag="LR110_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL4822637.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13038..15530)
                     /locus_tag="LR110_001675"
     CDS             complement(13038..15530)
                     /locus_tag="LR110_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBL4822638.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15537..16004)
                     /locus_tag="LR110_001676"
     CDS             complement(15537..16004)
                     /locus_tag="LR110_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBL4822639.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4737 a         3371 c         2868 g         5161 t
ORIGIN      
        1 cagtacagat actacctcta ccacaagtga tagctcagat tcatctacca ctacaagtga
       61 tagcacggac acgtcagctt caagtgacag tacagataca tcctctacca caagtgatag
      121 ctcagattca tcaacttcaa gcgatagtac agacacgtcg tctaccacaa gcgatacacg
      181 tcatgcaagt gtctccttct caaaaacaag ttcggataac aggacgaact ttaacttaac
      241 taataaaaca agcaatgaca atggaaacaa atcaaaaaga gcacttccta aaactggaag
      301 tcaatcgaat aattggataa cactcgcagg cgttatatta cttgtaatcg gtttaagaat
      361 cacttttatg agttataaga aatcgaaaag gtaaaaaaat attcaagata aaaaagagca
      421 acgtcgctaa cggacgttgc tctttatttt tgctttttat cataaataca agaactacaa
      481 ttcaatttgt caaaaaacac gtagatttcc ttctgagaaa tttatttacg tatcgaacct
      541 ttccctattc agtctttgcg atccaaaatc agttgggtaa gggaaaagag attctctttt
      601 gttcgaagag aggtggcagg taatttcttt atttccttca aagccttttc tgtgtaagag
      661 gccgctaact tcctagcttc ttccacgcct cctgtatgtt caacgatttt gcgtacggcg
      721 tcgacatctt cttctgtcat tgccgctttc ttctctaaat aaggtagtag ctttggtttg
      781 tttttttgca aagagtagat caacggtaaa gaatagaccc cttgacgcat gtcttctagt
      841 acaggtttgc cgatttcctg gctctctttt gtataatcta aaatatcatc gataatctga
      901 aaggccatcc cgatagaaag tccgatatct cctgcttttt ttgcgaagcg ctcgcttgtt
      961 ccactttcaa aagcgccaac agaacaactt aattggaaca gttcagctgt cttacctgaa
     1021 atattttcca aatattcttt tacagaaagt tcataattat agcgatcatc catctgccct
     1081 aattcgccat tcaatacttt ttccatactg cgagaattca attggagact tttcaaggaa
     1141 gtggagtaat cagacaatag tttaaaacag cagacaaata aatagtctcc tgcatagaca
     1201 gaaacgacat taccaaactg actacggata ttaggctgac cacgtcgcag atccgcttca
     1261 tcaacgatat catcatggat cagtgtggcc gtatggagca tttcgataga agctgccaaa
     1321 gcgatggctt tgtctcgatc ttgctctggt ccaaattgtg agaacaatag ctgataggca
     1381 ggacgcaaca gctttccgcc ggcatggatc gtttctttga ttgcttgttc gacaggtttg
     1441 tttttcaggc ggacagtttc ttccatccgt tttagtgttt ctgtcaattc tttacttaaa
     1501 gcaggatagt tttcccacat tggatgtagc ttcataaaaa aactccttgt gtgacgatta
     1561 ttttttggta aacattgggc tccatttttg tttggttccc tgctcataat ctttttggaa
     1621 tcgttgaagt gtctgcacat gtcgtaaaag ataattcgct gcgatcccga ttgcaatccc
     1681 tgataaaatc cctaaaactg aaaggactgg caagtacaac atgactgtcc atgattgtgc
     1741 aatgaaagaa gccatcaaaa gctgtccgac attatgcatg aaaccgccgg ccgcactaat
     1801 tccgatgata ctgacacgtt ttggtcctaa ttgcttgacc aataacattc caaaataact
     1861 aagcaaagca ccactcatac tataaaggaa tgtggaaatc gttccgccta gcaaagtagt
     1921 caggaatagt cgtagccaaa gtagtaagaa gctgtctcgt tttttcatgg taaaaatggc
     1981 aacgatcgtg attagattgg ctaagcctag ttttgcacca ggcgcaaaag aaaaaggata
     2041 agggatcata ttttccaata aaccaatgat cactccttgt gcaacaagta gcgaaatata
     2101 aatcattttt tgtaatttac tcatggtgtg tgaaggtcta ataaatcaga ctgtcttcgt
     2161 cacctccatc tgttgaacgg acttcgatca ctaatttatg tggcaaacag acgatggttt
     2221 cgccattttt cgttgcccag cctcgacgta cgcagatctg gtcatcgcaa tctgcttctt
     2281 ttatccgtac tcgatcgcca tctatctcga tcaagttata atctccgtgt tcatctttgt
     2341 aagtatatgt gttgctctct tctccagcca tcaaagggaa tgttttgatt tctgtgccat
     2401 ccacacgcag taccgcttct ttttctgctg ttgcggtgtt ttgctgaaga ccaaaaatca
     2461 tgactggtaa aaaagaaaga acagtcaaaa gcaaaataat aaaaacatcc caaggtttga
     2521 gcagactttt ttcagcaaat tctttaaggc tcatttttgt tctccttttt ccagattaac
     2581 tcacccaatt ttagcataaa aacaagaggt tgtgacacct gtgtcttgct ttgagtaata
     2641 agaacgaaga atagaaaaca gctttccatg ttttctaatt ctttgggctt attaccgaga
     2701 agcagcaggt ggaacaccgt ttataacaag aggttgtgac agaagtgggc aacttcaaga
     2761 aataagaagg aatttacgaa aattactttt caagtttttg tgaaatttcg gcttattttt
     2821 cgaagaagct acttctggaa caccgtttat aacaagaggt tgtaacagag gttgtgacac
     2881 ctgtgtcttg ctcatggagg cttatctcgg gcatcttatt cctttaattt tacataaaaa
     2941 ggaggctgtg acggtttgtc tcagcccctt tctattgaaa tcttgcattc aaataacgtt
     3001 ctttatctat ttatttcttg ccgtactggg aacttggtct accgtaccac caattaccta
     3061 gtgtacgttg tagccatgga attacccagc gatccaaccc aatcgcacgt ccggaaccgt
     3121 tcatcaatgc gaaagcaaca aaaatgaacc aaatgtttac ccagtaaaac atgcctgata
     3181 agcagaacgt gatcaccaat acaatcgttg tcgcactgca tagccatgtg aataatccag
     3241 caatcaatgc taatgccaag gcaatttcaa agaataccat gaatttttgg aagaacattg
     3301 ctacatcatt gtttggcatg atcacttgca tgatctttgt gaaccagtct ggtgacttct
     3361 caatcacagc cattggttct tcaccgtatg cataactcaa accaaaatgt gcagcttgag
     3421 tcgctgcttc tccggcactt gcccctgctc cgctggcagc tgtggttgca tcaccagcag
     3481 cagaagcacc actggcaaca tctgttacat tgtattccca tgtccaaggg aacacactgt
     3541 ttccagtgaa ccatgatcct tcaccaaacc aagtacttgg tttgaacact tcaccattcc
     3601 caatgatttt tttggaagct tcaacgaacc aaactaaacc atagaaaata cgaagtggca
     3661 cactccataa gacattgccg taacgagaag aatgtcctcg agtcacgttg cgatcatctt
     3721 tgatatggaa aaactcatgc attaaatatt ggaacatata ataaccagaa cgaatatcaa
     3781 agaagtattt caaattcacg atatgtttca ttacgatagc taaaaatccg cttaaatgga
     3841 tcttatcaaa taggttagct accccccatt tggcaccaat agaaaccata aatccttgat
     3901 agtttccttt aaatttatgc ttttctccgc ctttgatcga agccacgata ttcgctgcgg
     3961 ctgtgtgtcc tgtttgttca gcagcttgaa cgatctgcgg tgtcggtgtt cctgggaatt
     4021 cttcataaaa gactaaatca ccaatgatat agatgttttt atcttcgtag ccttttgcct
     4081 gcatatattc attggcgatc aaccgctgtc cgcgtgctga ttctaatcca aagtcagcag
     4141 cgtcagaagt tcctttgaca ccagctgtcc aaatcagtgt gtgagtcgga atgttgcgtc
     4201 cgtctttcaa tttgatatga tcagcagcca cttctacgat tggtgcattc aataataatt
     4261 ccacgttgtg cttacgcaaa tagcgttcag cttttgcagc atcatttcga tccaacatat
     4321 tcaagatcgt cggcattgcc tctacgacca tcagtgtgat ttcgtttgga tctaatttat
     4381 aatctttggc taggcgattt ttccagtcga tcagttcccc aatcatttca ataccggtaa
     4441 atccagaacc acagacaacg aaagtcagca tggctttacg cagttcatca tctggttcca
     4501 atgcagcttt tgctactgtc ttctcaatat gttcccgaat ccttacagaa tcttcaaatg
     4561 accaaagggt gaatccgtgt gctttgacac caggtgtacc aaagtcattc ggctctccgc
     4621 ccattccaag gatcagctga tcaaaaggat aggaacctaa tttggtcttg acgatttttc
     4681 gttctttatc gataccgatt acagtgtctg tgacaagttg tacattcttt tgacgagcaa
     4741 acaaatgctg cagatcgtat tggattgctt ctggttctac acgtccgcca gctacttcat
     4801 gcaactcggt catcatagta tggtacgaat gacgatcgat cagagtaatt gctacatctt
     4861 cttttttcag tttctttgcc aagtattttg tagctgaaac accggcatat cctgctccta
     4921 caacgacaat attttgcttt gccattgatc atccgctcct tatagactat ctatataatg
     4981 aatatatgat aattctttta gtttgatact tattacacaa tctccatata ttatatagat
     5041 gttctgttct tttgtctaac tccttttgga tttttttcaa attttaaaat aaagcgtttt
     5101 ttcacgaaat ttgctgaaag aaaacgtttg aaatattaca tgggttattt atcaaaaata
     5161 gttgacttat aggctaaaaa gattagaatg tataatgaat cacaaagaaa aattgtggac
     5221 tagaaatgga atggtggggt attaaaatga agaaaaaacg ttttgtaact ggatttgccg
     5281 tattagcatt ttcagctcta gtgttaggcg cttgcggcgc agatgacaac ggaagctcaa
     5341 actcttcttc tgaatcatct acagcacaat cttcgactgc aaaaagcaca acagaatcat
     5401 ctgcaaaagt tgtagctggc ggagatttgc aagacggcac atataaatta gaagaaaaaa
     5461 attattcaaa tggttaccgt gcagtctttg aaatggttgt aaaagacggc aaaatcactg
     5521 aatcaaaata tgataacgtc aatgaaaacg gcgaatcaaa aacaaaagat gcagaataca
     5581 acaaaaacat ggaagcaaaa tctggcacaa acccagaaaa atacattcct gagttgaatg
     5641 accaattctt aaaagcacaa agcgcaagcg gtgtggaagt cgtaactgga gctactcact
     5701 catctgaaag cttccaaaac tacgcacaac aattgatcca agctgctcaa gctggtaaca
     5761 cagatacaat cgaaatcgac aatggtgctg atttgaaaga cggcacttac aagcttgaag
     5821 aaaagaacta ctctaacggc taccgtgtgc aattcgaaat gacagttgct ggcggcaaag
     5881 taactgaatc aaactttgat tatatcgaca aagacggtaa atcaaaacaa gatgatacag
     5941 aatataatga aaatatgaaa gcaaaaagcg gtacagaacc aaaaacttac atccctactc
     6001 tgaacgacga attcgtcaaa gcaatgggcg aagaagatgg ctcacctgct aatgtagaag
     6061 ttgttacagg tgctacacac agctcacatt cattcatcat gtacgcacaa caattagtga
     6121 atgcagctga aaaaggcgac actcaaacaa tcgaagtaga taatatcgta acagaaaaat
     6181 aatcacataa tcatactaat aaaagttgga gggtgggacg atagccagat taggcagtcg
     6241 tctcaccttt caaaatgaag agaggaaaaa gatgcggaaa aaactactat tttccttaat
     6301 cacactgggc atctctttta tggtgctaac tggctgcagc aactcagcaa ataaaacaac
     6361 cactgaaagt tcagcagcta ccaagatatt gaaagatcca tattctgatg aacagttctt
     6421 gttagggact tacgttcgta tccgtgtgta tgatgaaggc aaagaatcag cattgaaacc
     6481 tgccttcgat cgagtaaaag aacttggtga taaaatcacg atcaatcaaa aaggttctga
     6541 gatcgatgaa gtgaatgagc aagctgggat caaaccagta aaagtttctg atgacgtcta
     6601 cacgttagtc aagcgggcct acgaatatag ccaagattct caaggcggat tcgatatggc
     6661 gattggtgca atcacacaat tatggcgaat cggttttgat gacgccagaa aaccttccca
     6721 agaagaaatc gatcaggcat tgaaattagt ggattatcat aagatcgaac taaatgacaa
     6781 agaaaaaact gtttatctaa aagaaaaagg aatgatcatc gatttaggtg caatcgccaa
     6841 aggttacatt acggacgaag tagtgaaagt cttgaaaaaa caaggagtga ccactgccat
     6901 cgttgacttg ggcggtaatg tctatgtctt aggccacagc ccaagaggcg aaaaccaaga
     6961 ctggacagtc ggcatccaag atccgaacat ggctcgcgga tctgttttag gttctattaa
     7021 agaacggaac aaaacattag ttacctctgg aatttacgag cgttatttag aagtagatgg
     7081 caaaaaatac catcatttgt ttgatccaaa aacgggttat ccgttcgaca atgatatcgc
     7141 aagtgtcacg atcataacag ataaatcaat tgatggtgac gggttgtcta cagcagtctt
     7201 ttcaatgggt gtgaaaaaag gactggaata cgtcgaaagc gaattgaata acgggacgga
     7261 agcgatcttc gtgacaaaag acgataaagt atatgtgacc gatccaatca aagacacctt
     7321 caaattatcg gaggactccc attacacgat gggtgaccgt tctgaactaa aataaggagt
     7381 acgaaccatg acactaaaaa tctttttaga agtagttgaa atacgaacaa aggtggctag
     7441 tgtatttcct tttgcggtag gggtattatt ctctatcgct ttttttcatg agatacattg
     7501 gctaaacact tttttatttt ttattggaat gctagttttt gatttagcga ctaccgctat
     7561 caataattac atggattatg aaaaggctca ttctgaagta tataagtatg aagaaaatgt
     7621 cattggccgc agaaatctat ctcctgtatt gattcgaaac atgattttag ctatgattgc
     7681 atttgttttt gttatcggcg ttattctaac gatcaatacg ggttggcttt tacttttgat
     7741 gggatttatc tgttgtttta ttggagtatt ctatacattc ggaccgattc ctttatctcg
     7801 aatgccttta ggggaaattt tcagtggggt gactatgggc cttggaattt ttgccatggt
     7861 cgtttatgtc aatacattcg gttcagatgt ctatgatctg acagtcaact tttcatctgg
     7921 acaatttgct ttggtaggaa atctttgggc tatagcagca atcgtattag cttccttacc
     7981 cttggtgttt acgattgcca atatcatgtt ggctaataat ttgcgggatc ttgaccgtga
     8041 tattgaaaat catcgctata cactagtttt ttatatcgga cgtcctgcgg gaacagtctt
     8101 attccagcta ttgatgtatg cttgctattt cgtgatttta atggggttgt tgagcggtat
     8161 ctatgaatgg ccgatcttgg tcaccttctt gaccttgcca attgtgaaga agcatttgga
     8221 tcaattcaag caaacgttac cccagcctat cagtttcagc tattccatca aaaatatggt
     8281 cttattcaat gccagttatg tattggggct attactaaca atcattatgc aagccattta
     8341 acagcatgaa gaggttgtga aaaaactgcc ctgcatcaag taataagaac agccaaataa
     8401 aaatagctcc ttatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa
     8461 caccgtttat caggaggttg tgaaaaagtc gcttagtttc gagtattaag aaaaattttg
     8521 tgaaaatggc tttccacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg
     8581 aacaccgttt atcagaggtt gtaaaatcag tgttttgtcc tgagtattag agaaacaaat
     8641 ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgaagga cagctgattg
     8701 aacactattt gttcggaaaa agtgactaac tatgacacaa tttttttcac agtccctttt
     8761 ttcacttgat ttgattcgta ccagcgaact tgacgagaac tttcttggtt tggtaactat
     8821 atggtattct tttaacatcc tcgtttgctt cttctaattt atttatacaa agtcacactt
     8881 ttttaaacat ctgttcttcg atacatttct atttctttta aacatcgata ccaatatttt
     8941 gaacttaaga gtgcatacca aatggattca tttactcttc gtcagttacg aagataatca
     9001 aaatctccaa cttccctact tatttcgttt tgagaattat cgaagcaatc ttacataaat
     9061 aactaaaaaa caaaaaacca gaaaatcagt atactgattt tctggtttta catggtttgt
     9121 tcaataaaaa gctgttggaa ctgttaatat taaattatcg catcgttacg aattcttccg
     9181 ctcctgttgg atggatggct actgtattgt caaagtcttc tttagttgca cccatcttga
     9241 tagccacggc aaaaccttgc agcatctcat cgacaccaac accaatagca tgtaagccga
     9301 cgattctttc ttcttttccg acacagatca atttcatttc gcatttttgg cgataatcat
     9361 ttaatgcaaa gtacattggg gtaaagcgtg aacgataaac tttcaattca ttttcgccat
     9421 atttttccaa tgcttcttct tctgtcaaac caattgttgc aattggtgga tgcgtaaaga
     9481 cgactgtcgg aacaagttta tagtcaagat aagaatcttt ctttccatta aataaacgtt
     9541 ctgataaacg tctaccagct gcgattgcaa caggagtcag atctaattta ccgatcacat
     9601 caccaacagc aaaaatatgt tcatccgttg tattttggta tttatctact ttgataaacc
     9661 cttttttgtc caattccaca tttgtatttt ccagacctaa tttttcggta ttcggtacac
     9721 gtccaccggc aaaaagtaca caatcaccag ttaaggtttc accattttca aatagcaccg
     9781 tatattcgtc attgttttta gtaatttctt tagcaatatg atgtgcgtaa gtttttattc
     9841 cagcttcttg gtacatttgg atcaaattgt ccgaaagcat tttatcaaac gttctaagtg
     9901 gtcgttcttt tcgatatgcc cacatgactt cactacctaa accacgaaat acacctgaaa
     9961 gttcagccgc aatgtatcca gcacctaaaa cgattgctct cttaggtaac tctgttaatt
    10021 caaaaaatcc atttgaatca attgcgtatt ctccaccagg aatggtcatt ttacttgcac
    10081 ggcctccagt agcgatcaag attttaggag cacgataggt ttcaccgttt acttcgatcg
    10141 tttgactatc gataaaagta gcgtaacctt ttatagcctc gacatgattg ctatctaatc
    10201 ctctttgata agcaccgtgt agaaaatcaa tatatttttc tcgtctttct actagaccgg
    10261 cgaaatcaac attttttaaa tctgcttgga taccgtaact tgaggcatct cgttcaattg
    10321 tttccaaaat cgtacttgct tgccacataa cttttttagg tacacaaccg acgttgacac
    10381 atgttccgcc caattcattc gcttcaatca gcaaaacttt tgcgccatgc atccctgctc
    10441 tgtttgctga agcaataccg ccagagccgc caccaattac gatgtaatca tatatttgca
    10501 ttttgttcct ccttattgaa gagatcaatc aaaaccttta tttaacgatc ctttttctac
    10561 agaaaacaag tgtaacactt ttcttgaatt ttgtatgcga agatgcttaa aacagggata
    10621 tatcagcttt ttccttattt acgttttcgg atcatatctc caggtttcac cggctgtgcg
    10681 actggcatag ttaaaatcat cattggattt ggtgctcggt caatggattc gccttcttca
    10741 ttatgcataa cttcaacggt ttgactgaaa tgactgaatc ctggaccata aaattcgatt
    10801 tcatctccta cagaaaacaa gtttctctga cgtatcgtag caatctgtgt tgcctcatca
    10861 taagataata cttcaccgac aaatttatat actgggattt tacgacgttc accgaataat
    10921 tgttcgtttt cagttggtgt attgtagtaa aaacctgtag ctaattctcg ttgtgctact
    10981 ttccataact cgtcgatcca ttcttgcttg cagacatagt tttcaggatc ttccatataa
    11041 ctgtctacag ccgctttata gacatttgac accgtagata cataatggat cgatttcatt
    11101 cttccttcga tcttgaagct gtctacgccg ttttcaatca attctggaat atgttcgatc
    11161 attgacatat ccactgcact catcgagaat tcttcttcca cttcgcctgt atcagttaaa
    11221 ctgtttcgtt cttgaccaaa aggcatatcg aacaatccat acttccagcg acaagattga
    11281 gaacatccgc cacggtttgc atcccgcatc gacatatggt tggacaatgt gcatcgacca
    11341 gaataagaga tacacatggc accatggata aatgcttcga tttcggtatc cgtattttta
    11401 cgaatctctg ccacttcctc cattgaaact tcacgagcta aaacgacacg ttccaaccct
    11461 tcttctttcc agaattccaa tgtctcaaaa ttagttgctg aagcttgagt ggataaatgg
    11521 atcggcagac ctggggcttc tgtcgcacaa atttcgatca aagctggatc agaaacaatc
    11581 actgcagaaa taccgacatc acgcaaactg cggaaaaatt cccctgctcc atcttcattt
    11641 ccttcatggg tgaccatatt agcagcaacg tataccttcg catcatgttc ttttgcaaaa
    11701 gcaactcctt cagccatttc ttcataagta aaattacctg cacggcttct taaaccatat
    11761 gcattcccgc caatatatac tgcatctgct ccataatgga tcgcggtttt taacttttcc
    11821 agtgttcctg ctggcgctag gatttctggt cgtttcaatt ttctttttac tgtcatttct
    11881 ttcatcatac tccttatttg atagcagaag gatcaatata aaagaatcct gtatctaatt
    11941 cacggttttc aggatgcaat ttactgattt cgctagtcat tttttccgct ttttcttgat
    12001 cccagctacc ttctaaaatt tgtttttttg cttcggcgaa tagtcgagtg atgtttacga
    12061 atttttcgcc tggagtaaat aatccgtcca atttccatgt acagtaatga tgctgataaa
    12121 gttcattcaa ttcattcgag agattcagat cattgctggc aaaaatatgt gtgccatgac
    12181 tatcttcata aatagaataa tgtgtctctt cttttttagg ttccgagata aaaagacctc
    12241 tttcacgatc tttttgttcg tcttgtttcg tatagttata gtaattctgt agcagtggac
    12301 gtttggattg atggatgcat gtagcaccat acacaagggt ttctacagga atatcgagtt
    12361 tttcttccat cgccttcatc tcttcaaaag gaacttcacg agcaagtact gctccaactg
    12421 cgcctttttt cgcccagaaa ttgatttgac gtgcgctcgt tactaatgtt tcagcatcat
    12481 aaatatacgg tatacgcaat tcaggatttt ttttcatcgt ataaataata cctgtatcac
    12541 caactgtgat cacatctacg tttatttctt ttaaaaaagc caaatattca ggtacatttt
    12601 tcattttttc aggatgaatg atcccattta cagcaatatt cgctttttta ccatacttgt
    12661 gtgctaattc taccaattgt cgctgttctt ctcgtgtaaa tgaagcaggc agacgcaatc
    12721 caaattgctc ttcaccgaaa tatagtgtat ctgcacctgc agcaagcaac tgttctgctt
    12781 gttcaatagt ttctactgtg gttatgagtt cgatcataat tttctcctcc tacaacttca
    12841 taatagtagc atagctctaa aaactttcca atacttcttc tatttaaagt cggatttttg
    12901 aaaattattt cacaaaaaat caaaataaag ttcgatatat tctgtccaaa tataaaaaga
    12961 gattgtgaca aaaatcttgt cacaacctct taatcaagtc ttactattat aaaaattcag
    13021 ctacacaggc caagtaacta tagggaacct actgtttcct ttttaggtcc gcgaacattc
    13081 aatgtgatct taccttttga agcacctact gttacgtggt cacctaaacg aatttctccg
    13141 ccaagcaaag cctcgctcaa tcgatcttca atttcttttt gaagtgcgcg acggattgga
    13201 cgtgcaccat attcaggatc aaatccagct ttcccgatca catcgattgc agatggtgtg
    13261 attttcactt gaatatcttg ttctttcaat cgtttgataa tcgctttgct catgatttgg
    13321 acgattgtat ggatttcatc tttactcaat gaatggaaga cgacagtctc atcgattcga
    13381 ttcaagaatt caggtcggaa agctttcttt aactcttcta agatacgttt ttgcatggct
    13441 ttgtgatctt tagtaacatc ttggacattg aatccgacat ttttttcttc tcgaatttgt
    13501 gtcgctccga tatttgaagt cataatcatg attgtatttc taaagtccac ttttctacct
    13561 ttagaatctg ttaaatgacc atcatccaat acttgcagaa gtaaattgaa aacgtctgga
    13621 tgtgctttct ccacttcatc taagagaatc actgaatatg gtttttgacg aattttttct
    13681 gtcaactgtc caccttcatc ataccctacg tatcctggag gagacccgat taatcggctt
    13741 gtactgtatt tttccataaa ttctgacata tcaacacgga tcagggcatc ttcacttcca
    13801 aacatgactt ctgaaagtgc tttggccaat tctgtttttc cgacaccagt tggtccaagg
    13861 aacatgaatg aaccaattgg acgatcagga tctttcaatc cgcttcgtgc acggcggatc
    13921 gcacgtgaaa ctgctttgac agcctcatct tgaccaacga cacgttcatg cagaagtcct
    13981 tccagttcta acaagcgttc gctttctttt ttctccaact gctgtagagg gacaccagtc
    14041 cactccgata caactgtagc aacatcttct tctgttacac gatcagaata accttgctcc
    14101 tgttttgctt caattgcaga aactttttgc agctttttac gtagtgcttt ttcttgtctg
    14161 cgtaattgag cagcattttc aaagtcttgt ttttggatcg ctgcttcctt ttcttgtact
    14221 agttcatcga tttctagttt gatcacagtt gattttgtta agtgatcggt ttgatccaaa
    14281 cggactttcg ctgctgattc atcgatcaaa tcaattgctt tatcgggtag ttgacgagaa
    14341 gtgatataac gaacagaaag attgactgct gctcgtacag cttcatctgt gatctccact
    14401 ccatggtgct cttcatatct tgtacgtaat ccttgaagaa tcacttctgc ttcttcagga
    14461 gttggctcat ctacttgaat gcgagcaaaa cgtcgctcta atgcagaatc tttttcgata
    14521 tatttttgat attcatctag ggtcgttgct ccgatcgttt gtaattctcc tcgagctaac
    14581 gccggtttca agatattcga agcatcaatt gctccttctg ctccacctgc accaattaag
    14641 gtatgcaatt catcaatgaa taagattact tgaccatcat tatagatttc gtcgatgacc
    14701 tttttcattc gatcttcaaa ttcaccgcga tatttcgtac cagcaaccaa agatcccata
    14761 tcaagcatca tcaggcgctt ttcttgcatg tcttctggca cttcacctaa aatgattttt
    14821 tgagctaacc cttcagcaat ggctgttttg cctacaccag gttcacccac cagtacaggg
    14881 ttattctttg tccgtcggct aagaatctgg ataagccgtt tcacttctct cgaacgacca
    14941 acaactgggt ccagcttgtt ttcttttgct aatttcgtca aatcacgtgc taatgaatct
    15001 agcgtaggtg ttccttgcgg tgtttgccgt ttatcttgta catttacatt tctacgacga
    15061 ttcattcctt ttccagcaga atcaccagct agccccattt tttgtttcag aacttgacgg
    15121 gcttttgaaa ggctgatacc taagttgatc atgattcgag tagccatgat actttcatct
    15181 tttaataagc tcatcaaaat atgttctgtt ccaacttttt gagaaccagt tttcttcact
    15241 tcgctgctag ctaaagcaaa aacttgtttc attcttggtg aatatggaag aaagactcct
    15301 ttagggtaag aacggatacc accataacca atcagatgtt caatttcttc ataaatttcc
    15361 gtttcattca aatccatttc acgtaatact tttccagcaa ttccattttg ctcgaccact
    15421 aaggctagta aaacatgttc tgaactaact gattgatgtt tcaaccgttt tgcttcttct
    15481 tgtgcaattt ctaatactgc ttgggctctt tcagtaaaaa gttcttccat ctaatctcac
    15541 ttcccatctt cataacttaa acgttccaga aatgctcgga gaatccgagc acgtgtatga
    15601 tcttcatatt cattgaaatt caatgtgttt tttgcaattg cactcaacat caagtttcct
    15661 tctttttttg taattatttg gtcttcataa agtttttgaa taatagcaaa agcatttttc
    15721 tcactgattg tatcatcaaa taactgattt atctgctcaa gtaattgttt tttatctgaa
    15781 atccttactt ttgcaattcg tatataacca ccaccaccac gtttgctttc tactagataa
    15841 ccacgttgga tggtaaaacg ggtattaatc acatagttga tttgtgaagg aacacagtta
    15901 aagaggtctg ccatttcaat ccttcgaatc tcgatcattt cactattttc cagaattttc
    15961 tttatatatg attcaattaa atccgaggta ttctgatgtg ccatcttatt cattcccttt
    16021 gactaatatt gaccttaatt ataacgaatt tgataataga aagaaagaag aacgcttttt
    16081 gttaaataaa tcctttagaa tcaacaaaaa aaaatacgga atatactatt ccgtatc
//
LOCUS       DAFFSV010000059        16134 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFFSV010000059 DAFFSV010000000
VERSION     DAFFSV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.059"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            69..1496
                     /locus_tag="LR110_001677"
     CDS             69..1496
                     /locus_tag="LR110_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4822640.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1588..1953)
                     /locus_tag="LR110_001678"
     CDS             complement(1588..1953)
                     /locus_tag="LR110_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL4822641.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1950..2315)
                     /locus_tag="LR110_001679"
     CDS             complement(1950..2315)
                     /locus_tag="LR110_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL4822642.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2507..2755)
                     /locus_tag="LR110_001680"
     CDS             complement(2507..2755)
                     /locus_tag="LR110_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822643.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2754..2882
                     /locus_tag="LR110_001681"
     CDS             2754..2882
                     /locus_tag="LR110_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBL4822644.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2939..4633
                     /locus_tag="LR110_001682"
     CDS             2939..4633
                     /locus_tag="LR110_001682"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBL4822645.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4637..4756
                     /locus_tag="LR110_001683"
                     /pseudo
     CDS             4637..4756
                     /locus_tag="LR110_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5284..6417
                     /locus_tag="LR110_001684"
     CDS             5284..6417
                     /locus_tag="LR110_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBL4822646.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6448..6768
                     /locus_tag="LR110_001685"
     CDS             6448..6768
                     /locus_tag="LR110_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBL4822647.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6797..7261)
                     /locus_tag="LR110_001686"
     CDS             complement(6797..7261)
                     /locus_tag="LR110_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4822648.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7393..8646)
                     /locus_tag="LR110_001687"
     CDS             complement(7393..8646)
                     /locus_tag="LR110_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4822649.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8675..8992)
                     /locus_tag="LR110_001688"
     CDS             complement(8675..8992)
                     /locus_tag="LR110_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL4822650.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9018..9338)
                     /locus_tag="LR110_001689"
     CDS             complement(9018..9338)
                     /locus_tag="LR110_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4822651.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9802..11217)
                     /locus_tag="LR110_001690"
     CDS             complement(9802..11217)
                     /locus_tag="LR110_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL4822652.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGRKYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11234..12550)
                     /locus_tag="LR110_001691"
     CDS             complement(11234..12550)
                     /locus_tag="LR110_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4822653.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12699..13397)
                     /locus_tag="LR110_001692"
     CDS             complement(12699..13397)
                     /locus_tag="LR110_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBL4822654.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13686..14642)
                     /locus_tag="LR110_001693"
     CDS             complement(13686..14642)
                     /locus_tag="LR110_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL4822655.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14755..15588)
                     /gene="msrA"
                     /locus_tag="LR110_001694"
     CDS             complement(14755..15588)
                     /gene="msrA"
                     /locus_tag="LR110_001694"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL4822656.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15763..>16134)
                     /locus_tag="LR110_001695"
     CDS             complement(15763..>16134)
                     /locus_tag="LR110_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822657.1"
                     /translation="YDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTN
                     EKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTII
                     SYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         5023 a         3255 c         2747 g         5109 t
ORIGIN      
        1 tttcaaaaag aaaacatgtc ataatatttc ccatattgtg aaaaaaaata taaggcggag
       61 gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg
      121 ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt
      181 gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg
      241 cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat
      301 gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt
      361 tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca
      421 aagcttattc attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg
      481 accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt
      541 atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc
      601 gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa
      661 tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag
      721 attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat
      781 gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac
      841 tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac
      901 agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg
      961 aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc
     1021 tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa
     1081 atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc
     1141 agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga
     1201 gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg
     1261 ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg
     1321 caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc
     1381 gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc
     1441 ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg
     1501 ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat
     1561 ctcttgatac acgtcaggct cacaacctca cttattttgg acatctaggg gtccgatctc
     1621 tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat
     1681 atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa
     1741 acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc
     1801 gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac
     1861 accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata
     1921 tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct
     1981 ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt
     2041 gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg
     2101 actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta
     2161 cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga
     2221 acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc
     2281 cattctagaa attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt
     2341 tgttcctatt cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct
     2401 aatgataacg gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca
     2461 taaatcgtgg aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca
     2521 gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac
     2581 catacacctc ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag
     2641 acacttcttc ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta
     2701 ataaaaacga actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta
     2761 aaaaattaat caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag
     2821 gaaaaaagac aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct
     2881 agcatcctat ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat
     2941 gagaaaaagt aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta
     3001 tttgattcaa gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca
     3061 cggttcaaat gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca
     3121 tccattagaa aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac
     3181 ctatgtagat ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac
     3241 tgctgaagaa gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt
     3301 gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg
     3361 tattcttctg actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat
     3421 cactatcgaa gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat
     3481 gtgggaaaca acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat
     3541 gcatcaaatg atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg
     3601 ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg
     3661 caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga
     3721 tgaaatcact gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt
     3781 tgtggtcgat gaagatacag cagttatttt cactaatggg tccattacag attcagctac
     3841 tttaggagat tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct
     3901 ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga
     3961 tcgagatgca agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa
     4021 tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac
     4081 cacaccgatc acgccgttag gccaaaaaga tgtcaatatg tctatcgtcg tgcatcatca
     4141 accgcatttt acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc
     4201 aagacgacgt ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct
     4261 agaagaactg attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa
     4321 agaaacagaa attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc
     4381 cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac
     4441 caatctggcc tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca
     4501 aagtgctgta cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg
     4561 tcttgtcaaa acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa
     4621 aatgtttgaa taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac
     4681 caggctaaaa cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc
     4741 ttttgaacac cgtttaccaa gaggttatga aaaagctgcc ctgcatcaag taataagagc
     4801 agccaagcta aaacagcttc ttatgttttt gacttggtta gacttattac cgcagatgca
     4861 agcttttgaa caccgtttac caagaggtta tgaaaaaggc gtttagtttc gagtatgaag
     4921 aaaaattttg tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa taccgagaaa
     4981 ctgatccctt ctgtcataac ctctttttat ttatgtctgt ttctttttga aaacaactat
     5041 tttttcaacg tttactctct tgccaatcca acatcatcta acggatacaa ctatttcaat
     5101 gattaccatt acactaaaaa atcggaaaca cacctctccg aacacaaata cataagacta
     5161 tagaatgttt catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa
     5221 aatgaaatta tctgtagtac taataatgca gacaatttta tgaatctgaa aggagcatct
     5281 ccaatgaaag cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat
     5341 ccaaaaatat tagaaccaac agatgcaatc atccgtatca cagcaacagc catttgcggc
     5401 tccgatctgc atctttacca tcatggtgaa gcagttttgg aaaaagggta tgttgtagga
     5461 cacgaaccaa tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg
     5521 caacgtgtgg ttatcccttt taatattagt tgtggtcatt gtcatttctg cctgaatcac
     5581 atggaaagcc aatgtgatga atcaaatcca gatcagttat atggcgggct tttcggattt
     5641 ggcaagttga acggtaatca ttggggtggt caagcggaat atctaagagt tccttatgca
     5701 gactctactt catttgttgt accagataat gatcttcctg atgaaaaagt tttatttcta
     5761 tcagatattt tgcccacagc ttattggagc gttgaaaacg caggagtaaa aaaaggcgat
     5821 acagttgtga ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg
     5881 gctggagcag agcgcgtaat cgcagtagat ccagtacagc accgtctaga taaagcggct
     5941 aattacaatg gggttgaaac ttatttctta gaagacacgg cacaagctgg aacagaattg
     6001 tatgaactgg ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat ggacggatta
     6061 gagcctgtca aagaaaaagc gaaaaattta gtaagcttac aaagcggcac aatctctcca
     6121 ttacagatgg catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat
     6181 atgactccag cttcttctta cccgctgcaa gaatttttta tgcgggatat cgctgtcaag
     6241 catggacagg caccaattat ccatctaatg ccaaaaattt acgcaatgat tgcagaagga
     6301 ctattcgatc ctacacaaat cattactcat tcgctgccac tatcagaagc agcacaagca
     6361 tatgagatct ttgacaaaaa agaagataaa aatataaaag ttgttttgaa accttgaatt
     6421 cagtaaaaaa tacaggtgat tttctttatg cccaaaatca aacgaaatac ccatgagatc
     6481 gaaataggaa aagaagaaga tatcgaaata cgaggacgtc gcttccgctt ctatttccaa
     6541 aatcgttaca cattgatctc actggctgtc gatttattaa ccgggatttt ctatattttt
     6601 ggaagccttg cttccttaac taaaatcccc gatcgatatg gcacctattt ttatttggct
     6661 ggtgctattt tcttgacgat tcgtcctatt ctcagaatcg taaggaacat ttttatttac
     6721 gacgaaaaaa aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa
     6781 tctcatgcct cttttttcac cctatcgtat cactttgata cagacgagcg gtcgtcccac
     6841 tttcttcctt gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat
     6901 ctgtaattaa gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg atttccgcta
     6961 atatccgctt ggctatcccc tgcttacgct tttccggttc aacaaacagg gcacaaagat
     7021 ttggtgtcag atcagggcgg ttatggaagt cgttttcaat gacgcccgct cctccgacga
     7081 tggtttcttt ttcatctaag accacatacc attgaggaat actttcctta ttagcgattc
     7141 cagtacgaat actttcctca tacacaacag caggaactcc ccattttgat gaaaaccaat
     7201 tcgcgctttg ttttactaat tctgggtgtt ctcttaattt gatccagtta tatgtaacca
     7261 atgtttaaac ctccgactaa aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa
     7321 ttagcaatga caacaatagt gatgtaaaaa aaaaaacgga aaacaatcac ttgttctccg
     7381 ttttttgatt aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt
     7441 gacgaacggt agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc
     7501 tgcaatactt tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat
     7561 aacggtagct gctggcacta aacctgtaga agtcgcaaag taagcaacac taactaagac
     7621 cattggtgtc aaaataaatg gaatgaacat gatcgggttc aacacgatag gtaacccaaa
     7681 catcgttggt tcattaatat tgaagacacc tggcgcaatc gacaagtttg ttacaaccat
     7741 atatggtttg tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc
     7801 tgttcctcct aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct
     7861 atgtccagct gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat
     7921 caaaggatcg accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa
     7981 tgcaagcagt aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac
     8041 tgtatagaaa gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa
     8101 agcaaacgca gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc
     8161 cggtggcaca ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa
     8221 gatttctgtt gaaataatcc caacaataat agcaataaac aaaccgtttg agctcatacc
     8281 taatgctccg cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc
     8341 ttttcggtag ccgttagcta aatgataagc aagcaagaaa gcaatcaaga cagaaagaat
     8401 ggcaaatgta ccgttccaag cagcagcccc gaatcctttc catgattcac caccaaaaat
     8461 tgagttcatc aattcttgga acccgggaat cggtaagtta ttgatcatta ctgctaatgc
     8521 tcctaagatc attaacggca tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg
     8581 ttgtgcacca atttttgatg ctacaggtat aaaatgtttc tccataaatg caacaaattt
     8641 atccataaat cttccctctt ttctttactg tagattacgc gttcaatcga cgatacagat
     8701 caatgatctc ttttgctaaa tctgtaaaag ctatagatgt catcagatga tcttgactgt
     8761 ggaccgtcaa aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg
     8821 tttgagagtg atgtgcttgg actaaagatt cctctgcatc tttgattttt tcatctgcta
     8881 aagtgaaatc accttttttc gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc
     8941 cgccaaacat gatcagaccc atgatggctt ctagattttt ttgatcttcc atttattgta
     9001 tcctccgata atttttatta tttgtttatt agtttttctg cttggtcgag tactttttca
     9061 ccattcatca tgccatagtc agccatattg attacatcta aaggaatgcc tttaggtgct
     9121 agcttttcag caaattgatt ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct
     9181 acatttttag attctagttg attatccgca tctgaagcag atacagcaaa gatgtctgct
     9241 tccattcctt ttgcttctgc tgccttttgc atttttgtga ctaggagact tgtactcatt
     9301 ccagctgaac atacaagcat gattgttttc ttttccattt taaacacgtt cctttctata
     9361 cttacacttg tatataagca agtagcgtgc caactttaaa agcgctttaa aaagttgata
     9421 tcatcacatt taaggtctat accaagttgc atacacacta aaaaagaaag cgcacactaa
     9481 acacacaaat taatttttat tacctttttt cttttaacgt gcacaaaaaa agttgtgaca
     9541 caaaacttgt cacaactttt ttcaaataaa cggtattcaa aagcttgcat ctgcggtaat
     9601 aagtccaacc aagttaaaaa cataagatgt tgtttttact ttgctattct tattacttga
     9661 tgcaggacag ctttttcaca acctctcaat aaacggtgtt caatcagctg accctgggta
     9721 ttagctcaaa acgctggatt cacaacctct tttatttcaa ataaccagta aatacaatga
     9781 acatctacaa aaaacttcta tttatttata gatttcgtca ggcaaatcca attctctctt
     9841 atcaatcaaa gtctgatacc acgaagcaga ttttttcaat gttctgcttc gttcatcgtt
     9901 caattcgatt cgaactaatc catatcgatt cttaaatgca ttcattggtg acacacagtc
     9961 agtgaatgcc cataacatat agccttcgca atttgcgcct tcttctgttg ctttgatcaa
    10021 gtaatgcaaa tgctgtccga taaattcgat acggtaatca tcttgcacga ttcctttctc
    10081 atccatatac tgttcttcat attttcttcc cataccgttt tctgttatca gccaagggat
    10141 attatgataa ttttctttta gatacatggc tatatcatac atgattttcg gatagatttc
    10201 ccatcccctg gaattattca ttttcttccc aggtaaatca aagacttcat aatatcgttc
    10261 aggatggaaa ggtgtctctt cattccattg ataacgaggg gcgcgaacgc gtttaggata
    10321 atattgattg attccaagga aatctactgt atgatgctga atcgtttcta aatcctctgt
    10381 tgcaggatcg aaataaatcg attgtttttg acacaactcc aggaactctt ctgtgtagta
    10441 tccttttacc atcggatcaa aaaatactcg attataaaac aaatcataca tataagcagc
    10501 tttttggtct tctggagaat tggatcgagc atagaccatt tcaggattta aaatacagcc
    10561 aatccttcca ccataatttc cgctgcggta aatctctact gctttagccg ttgctaaaac
    10621 tttatgataa ttccacatca tccacttttt tgtgttttgt tcatgcggcc agcgaacagc
    10681 atccaaatag caacgcgttt ggggaacgat tggttcgttg aatgtaaacc agttttttac
    10741 cctatcaccg taacgatcaa aggcaatttg agcatactta gcataaaaat caactacttt
    10801 tcttgaagac cagccatcat atttttccaa aagaaaagct gggacttcat aatgttccaa
    10861 acaaatcatc ggttctacgt ctgcttcgat caaagagtcg atcatttcac ttatatgctg
    10921 agcatattct tcatctacta tacagttctc ataatcagta aaaaaacgag cccaattgat
    10981 agaagtccga taatgtgtca gatggatgga tttcatcaga tccacatcct gctggtattg
    11041 ttccataaaa ttagttgcta cggctgggcc atacccatta tgccaaacaa aatgttcatt
    11101 tttataccag ctatctataa acgaatcctg tccttctttt tttcctttcc aaccttccgt
    11161 ttgccaagca gaagatgcag caccaagaat aaaatttttt ggaatagtaa ttttcatgat
    11221 ttcctcctgt tttttagatc ccatcatttt ccacacgatt tgctactttt acaaatggca
    11281 gatagatcaa aatagaaaca ataatacaaa caagttgcgt aataactgca ccgatatttc
    11341 ctgctgttgc cagataagca ttgacgatcg gcggcattgg ccaagggacc attacaactg
    11401 ctttcgccgc aaatcctaaa gcggttgcaa aataaccaat cgttccagtg ataagaggcg
    11461 ttaagataaa cggaatagct aaaattggat tcagcatgat cggcatacca aaaataactg
    11521 gttcattgat gttgaagatc ccaggagcaa gggataactt ggtgatttgt ttcatatctt
    11581 cccgttttcc aactaacaat actgcaatca gtaaaccgat cgtgactcca gacccgccca
    11641 tactcatata catatcccaa aatggcatag taataatgtt cggtacttct tttccagctt
    11701 caaaagcttc cgtatttaca gcaattgccg ctaaaagcaa cggttcccgt atcggcttca
    11761 ccatttgatt tccatggata ccaatcaccc aaaagacttg cgcaataaac atcaatagca
    11821 aaatacccgg taacccttgt acgacacctt ctaatgggcg ttgtacgaca ttgtaaatga
    11881 tatcataaag gtacattcct gtaagttctt tgatgataaa tcccgcagcc gcgatacccg
    11941 atacagtcag aatcgtcggg aataaagcag aaaaactagc agaaacattc gttggcacgg
    12001 tatcaggcat tctcagctgt agtttcttct gtttcctcaa ccagcaataa agttcaatag
    12061 aaaggatgga gaccaacatt cccaaaaata accctttcgt atctgtatat tgtttagcca
    12121 atacgttttc tactataaca gtctgatttt cattcactaa ttcataaaca gttggattaa
    12181 cagataaata agacatcact gcgatgatac cagggaatac accatccact ttattcagct
    12241 tggctaattc gatccctatc agaaaaacag catagatggt caagaaactt agtgtgacat
    12301 agctgatggc agatgcgata ggctttaatt cttctaaaaa acgcaagaaa cttatctgtg
    12361 ctaagccatt ttccgagtca aagaccattg ctgaaaacaa tgtgccaaaa gcacccgtaa
    12421 taattaccgg aatcaatgca gcaaatgcgt tttttatcac catgatatac cttaaattat
    12481 tgattctcgt tgccacgttc cctaaaacat tggctaattt atccataaaa ctcattccag
    12541 atcccctcat tcctgcttct tctcttgacg tcatctcttt ttgtctatta gtcagcgctt
    12601 acatttcgat gaacagaaac tatttactcc tcttttttac caaatataaa acaaaaattc
    12661 atctgatgta tttatcataa aatacatcag atgaactgtc aattagtttc tttttctcct
    12721 attattttct gtaattctct ccgatttatt gccatatgga cagtcattgc atcaaacgca
    12781 gcaagtgcat tccgcctttc gatggcctga acgatctctg tatgaatatc gatcgtctcg
    12841 attttcagat gctggatatc ataattttta ttaaacaaag caattgattc attaatgatt
    12901 gggataatat gcagcaaggc tacgtttccg ctagcttctg caatcatcgt atgaaaatcg
    12961 atgtctaatt gaacatgttc ttgtgtgcct tctcgaaaag aagtttcaat cttttgcgtt
    13021 aattttttca tttggacgat ttgattcttc gttgcatatt ccgctgccaa acctgccatc
    13081 ttaggctcca ataaatatct cagttcaaat aagtctcgta tcatccgatc ctgatcttca
    13141 atcaaagaaa aaccgagtgg gtcattggca atccctcgct tatcactgac aaatgtcccc
    13201 gctccttgcc gaacttcaag gatattcctg gaagctagag cccgcacagc ttctcgcacc
    13261 gtgcttcgcc ccacctctaa ataatcggac aattcatatt cattcggcag tttatctcct
    13321 acttgcaact gcatttcctt taaatatcta aggatcttgt cagtagttac ttctactagg
    13381 gttttatttt cttccaaacc acatacctcc ttttcatctg atgtattttt tcgtaattat
    13441 tataacacgt ttgaatcatc tgatgatttt ttgtatacaa aattgattaa tccgttcata
    13501 gacatacaaa cgaagaagat atttaccata aaaaagacta ggataaaatt cactcctagc
    13561 cttcgatcat ttttattttg aacgacaaaa aatatgatgt tcaataacag acgatcttca
    13621 ttcgattctt gatgtcatct tttgtttttt ttaatatatg gataaaaagt ttgattacat
    13681 cgtttctatt ctagaatttg gtcgattctc aattcttctt ctggtgagaa atctaaatta
    13741 tccatcattc gaacactttc ttgtaactga cttaaccgac ttgctccgac cagtacactt
    13801 gtaacagatt tttgtcgcaa attccatgca agggccattt gcgctaatga ttgtcctcgt
    13861 gatacggcga ttgtttgcag tgctttgatt ttttccagag ttgatccaac ttgttcttct
    13921 gaaaggaatg gtatttcctt acgatgtgct ctagaatctt caggaatacc atgtagataa
    13981 cgatttgtca gcaacccttg agcaagcgga ctgaacgtga ttgcccctag cccttcttct
    14041 tctaatactg gagatagatc gtcttctagt agtgcacgag aaaacatatt gtagcgcatc
    14101 tgatgaataa tgaacggtgc ttcctttctt tttaaaattt cagtcatttt ttttgtatct
    14161 tctccattat agttggatat accaatatat aatgcttttc cttgtcttac aagttgcatc
    14221 aaagcttctg ctgtttcttc caaaggtgta tcagggtctg ggcgatgatg atagaagatg
    14281 tcaacataat ctagccccat tctttggaga ctttggtcac aacttgaaat gatggattta
    14341 cgagagcccc attccccata tggcccttcc cacatatgat aacctgcttt gctagaaata
    14401 atcagttcat cacggtaggc ttgaaaatct tctctaaaaa tacgcccaaa gtttttctct
    14461 gcagctccag cagggcctcc ataattattc gcaaggtcga aatgggtaat tcccatgtcg
    14521 aatgcgcctc ttaaaatctc tctttgatta tggataggat cataatcacc aaaattttgc
    14581 cataatccga gagataaaat cggtaatttc aaacctgaat gaccggattt acgatattcc
    14641 attttttcat aacgtgcact atctgcaata tacaattttt ctcctcctca tcaaatcttt
    14701 ttaaaacgct ttcttttttc tatttttagt ttactacatt ttaaagaaaa ggagttaatc
    14761 aaaatgattt tttcgtatat ttttttgcca tttgtttttt actcgttgat agatcagaaa
    14821 ttgattacgt gcttgctgga tttttttata acgtttcggt tgttttttgt aaaattgctg
    14881 atgatagttt tctgctggcc aaaacgtacc ggctggttcg atttttgtga cgattggttg
    14941 gtggaaagtt cctgaactgt ctagtttctg ttttgattgt tccgctaatt ctttttgtcg
    15001 ttcgccttga taaaaaatca ctggacgata ttgcttccct ctatcttgaa attgtccaaa
    15061 tgtatctgtg ggatcagtaa tttgccaata gattgtcagc aactcgctat aacttatttc
    15121 tcttgtgtca taaatgatct ccacagcttc cacatgacct gtgtaaccac cacttacttg
    15181 atcataattt ggtttttcaa catgtccgcc cgtgtatcct gacaaaacag ataggatccc
    15241 ttttttcgat tcaaacggct cgaccataca ccagaaacat ccccctgcaa atactgcacg
    15301 ttcttgatag tcagcatctt gcatggcatg cttggaaaag tcatattttt gctccttatc
    15361 aaatccttcc gtaatcaata aataaaagtc cattacatca ggcgtcagat tcattcggat
    15421 agctaatgga cgtagtgttg cttctagttt tgataactgt tctttaacgg atatattttc
    15481 tcttatgtca ttctttgtct gaatcaatac ttttcgttcc caatctcgtg tattcggatt
    15541 caaaatcaga ttataaagtg tttgaatgaa tatttgttcc tcatccatag attctcctcc
    15601 tttccgaaga agacaagcta gtgactttag ctgtaattta tcataaagtg aggaaataag
    15661 cgattacttc atccccctac agatttttcg tataaaaaag agagaatcct tatcgacaga
    15721 ttctcctttt taatcaattt attttataaa tgaaaagtta atttaccttt tatggaaaac
    15781 tattttaaaa aataaacata aatatgcagt taatgtaaca agataagaaa taatagtcgt
    15841 tactgtataa cttgtgctta ctttaatatc agcaactcct ttgactccgc ttatttgtac
    15901 caatccatta gcattcacct gctcctttac aggttgatga tttatttcta cattcgttcc
    15961 taaataagca tatacaggaa gtgtgattac ttggctcttc tcattagtta ctctatatat
    16021 gatagaacta ccagttgttt tataaggaag gtttacccat tgactgccaa tctttgcttc
    16081 gtgttttatt atatgctcct tattctccaa tgaagcgata ggagaataat cata
//
LOCUS       DAFFSV010000060        15900 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFFSV010000060 DAFFSV010000000
VERSION     DAFFSV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.060"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(52..522)
                     /locus_tag="LR110_001696"
     CDS             complement(52..522)
                     /locus_tag="LR110_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBL4822658.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="LR110_001697"
     CDS             complement(681..1139)
                     /locus_tag="LR110_001697"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBL4822659.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="LR110_001698"
     CDS             complement(1310..2455)
                     /locus_tag="LR110_001698"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBL4822660.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="LR110_001699"
     CDS             complement(2468..3385)
                     /locus_tag="LR110_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBL4822661.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="LR110_001700"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="LR110_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBL4822662.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="LR110_001701"
     CDS             4477..4647
                     /locus_tag="LR110_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822663.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="LR110_001702"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="LR110_001702"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBL4822664.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="LR110_001703"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="LR110_001703"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBL4822665.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /locus_tag="LR110_001704"
     CDS             6911..7792
                     /locus_tag="LR110_001704"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBL4822666.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /locus_tag="LR110_001705"
     CDS             7789..8505
                     /locus_tag="LR110_001705"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBL4822667.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="LR110_001706"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="LR110_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBL4822668.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="LR110_001707"
     CDS             9222..10223
                     /locus_tag="LR110_001707"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBL4822669.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="LR110_001708"
     CDS             10220..11581
                     /locus_tag="LR110_001708"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBL4822670.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="LR110_001709"
     CDS             11541..12692
                     /locus_tag="LR110_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBL4822671.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="LR110_001710"
     CDS             12667..13836
                     /locus_tag="LR110_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL4822672.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPVAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="LR110_001711"
     CDS             14011..15390
                     /locus_tag="LR110_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL4822673.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..15900
                     /locus_tag="LR110_001712"
                     /pseudo
     CDS             15671..15900
                     /locus_tag="LR110_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5200 a         2803 c         3386 g         4511 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaagaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tacagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 gggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gttgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DAFFSV010000061        15797 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFFSV010000061 DAFFSV010000000
VERSION     DAFFSV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.061"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..173)
                     /locus_tag="LR110_001713"
     CDS             complement(<1..173)
                     /locus_tag="LR110_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4822674.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="LR110_001714"
     CDS             complement(593..1714)
                     /locus_tag="LR110_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBL4822675.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="LR110_001715"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="LR110_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBL4822676.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="LR110_001716"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="LR110_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBL4822677.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="LR110_001717"
     CDS             complement(4304..5215)
                     /locus_tag="LR110_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL4822678.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="LR110_001718"
     CDS             complement(5232..6239)
                     /locus_tag="LR110_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL4822679.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="LR110_001719"
     CDS             complement(6236..8365)
                     /locus_tag="LR110_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL4822680.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8513)
                     /locus_tag="LR110_001720"
                     /pseudo
     CDS             complement(8370..8513)
                     /locus_tag="LR110_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(8666..9853)
                     /locus_tag="LR110_001721"
     CDS             complement(8666..9853)
                     /locus_tag="LR110_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBL4822681.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(9946..10764)
                     /locus_tag="LR110_001722"
     CDS             complement(9946..10764)
                     /locus_tag="LR110_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL4822682.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
     gene            complement(10898..12724)
                     /gene="ltrA"
                     /locus_tag="LR110_001723"
     CDS             complement(10898..12724)
                     /gene="ltrA"
                     /locus_tag="LR110_001723"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL4822683.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            complement(13469..14971)
                     /locus_tag="LR110_001724"
                     /pseudo
     CDS             complement(13469..14971)
                     /locus_tag="LR110_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(14958..15347)
                     /locus_tag="LR110_001725"
     CDS             complement(14958..15347)
                     /locus_tag="LR110_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL4822684.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            complement(15397..>15797)
                     /locus_tag="LR110_001726"
     CDS             complement(15397..>15797)
                     /locus_tag="LR110_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4822685.1"
                     /translation="LNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQ
                     LINNLIVRSIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPN
                     IKPRICTVMEGGEDAFTNRAKRYGFKQIVE"
BASE COUNT         4614 a         3323 c         2741 g         5119 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttttcagtca agtccacaat cttgtgtaaa
     8521 agttcaacct catcaaaagc acaatgtttt gcgttgtgaa cctaactgcc atgcctttct
     8581 ttctgaggag agaggtcagg agcagtagcg cagcgactct tgatgtctct cctcagaaag
     8641 aagagactcg cacaggttca tggtgctaaa tgttattcga attaaattct aaatattttc
     8701 gtggagaagc cagccactca gtgtcttttt ccatcaaaag tgttccaatt atccgagtga
     8761 tggaattgat atttggaaaa atccgaatca ctcgttctct ccggcgaatc tcttcgttca
     8821 accgttccaa cacattggtt gtacgaatcc ttcggcggat attttcagga aaagacaaaa
     8881 tttgaatagc atcttcaaag ccgttttcta aaatgtcgca agcagcactt aattttgaat
     8941 cacaaccata tttttccaag aagtgctctt ttctttcacg tgtcaattca agttcagagg
     9001 ctttaaagat actcttcaac tcgtctttta catcagaaga aactttcttt ggcagcttat
     9061 caaaaatatt tctcaaaaag tgggcttggc atctttgcca gctcgcacct aaaaagttct
     9121 tgcgaacagc cttaactaac cctttgtgtg catcagaaat gaccagtttt acaccgaaca
     9181 aacctcgttg aacgaacgat tgaaaaacgg tatcccaatt atcttccgat tcgccgtctt
     9241 gaacatcaaa accaagaatc gtacgtcgtc catcagaatc aatgccgata ccaatatata
     9301 caccttttga gacaatacga tgattttccc ttactttcat atacagggca tcacacatga
     9361 aaaatggata ttccgagcct tctaggctcc ggtttctcca ctcaaaaaca aatggatcca
     9421 actgtttcat cagatttgaa acgaatgatt tagaaacagt cgcaccatcc gttagcagtt
     9481 caactgtttt agttactttg cgagtggaga caccagaaat gaccatttca atcatggcag
     9541 cgatcaaagc tttttcgctt cgctgatatc tttcaaagat agtaggacta aacttaccgt
     9601 ctctggttcg tggaacatga agttctaacg taccaattcg agttgtaaac gaacgatcgt
     9661 agtaaccatt tctttgtgat tgccgttctt ctgaacgttc ataggcattt gcctgaatat
     9721 attgatttcg ttgttcttcc atcagctgat taaacatgat agtcataagt gtttttgcgg
     9781 tatcattcgc accgctttcg tttataattg cttgaatttc ttcgctttca aaagtaaaat
     9841 gtacttgagt cattgatatc agtcctattc gagtatgttt tctgcaaaaa acattgtact
     9901 cctaactgag atcaatggct tttttgatgc ttcactttta cacaattata tggacgtaat
     9961 cccgttttcc aaatctcgca ggcgtttttg attgctttga tcctcctgat ccaaaccaaa
    10021 gaattccaag gttttcttga tttcatgaat atcggtactg cgaaaagcaa acttcaaccc
    10081 gatattattt tttaaacgtt catctaataa atcgtcgcta ttttgagtga cgaaatagac
    10141 cccagcattc atggcccgac cagcacgaac aagcttattc gataacgttt ttccttgagc
    10201 cacttgaaga aaactccaag cctcatccaa atcgacaatt ttgaagattt ctcggttact
    10261 atggataaag tccaaggcaa aagtggaaat gacgattagc atcgaaacac taagcaattc
    10321 catggtggta tattcttcaa aagtcgtttc tgcatccggt aaaaccaaat ccgccacttg
    10381 aatgatattt agctgtttat ctaaactaat cgattgggta atcgagccgt ctgagaaaag
    10441 caaatgagca aaatcataat cggtaaagga ctcgatatga tcggcgatat tttccgcgat
    10501 tggtgacccg tcttttcgca attcgttaat aacttgtaat aacccccgtt gatcactttg
    10561 agtcaccgtt cgaatggctt tacgtaacac aggaaacttc tccccgtctc gacttgaaat
    10621 tcctgttaaa aaagtcagaa tatcaattgc taaactttcc gagtctttct ttttcttcat
    10681 aatcacataa ggatcaagca gaccttgatt ttctgactta ctcgtcaagt tgaggatact
    10741 gatttcatcc gcaatttctg gcaaggtttc tttccaccct cctcgctata gcgataggta
    10801 actttttgat gttatctcca agttttcccc cgcttcacac cgtacatgaa actttcgctt
    10861 catacggcgt tccatcatat ccaaatagat ttataagtta tttcccagtt attgtctggt
    10921 atccgtaatt cgtccgtatt ctgttcagtt ttttcttctg ctgactatta agaacataac
    10981 cttttaacag tgattttgct tcgtctgctg attctgttag gattgccttt gcaatgggaa
    11041 tttccacaaa tgccagatta gaatatctgt caaggttatc tcctttgtct gatttgtgaa
    11101 tacatatcag agtggacgga gttcctcgac gattcattac atagcagttg ccttgttgtg
    11161 cgatatatgc cgaaatacga ttgtcgttaa actctacgct atcggcaggg ttttcatttt
    11221 ccaacaacgc tcggatttct tcttttgtta caactacaac gtcctcatgc accttgtttc
    11281 tcccttgctt tgtaaaatta caaatatctt gcgagaaatt cataggattt ttgtgttgta
    11341 ctccttgaat cggcaatagc ggattatccg caatcatcac tatttttgtt tttggtctga
    11401 ttccttttgt cctgctctga aattctgaac ttgtactttc aaatggaact gattttgcaa
    11461 tgttccttaa acggattcgt atggtcggta agagagcgta acttacttct gtcaggttgt
    11521 tatatacgtt agttgctatg cagtagtaat tctgcatacc gataactgtc agattatatc
    11581 tgctgatatt gaagccagtc gtatgtctgg ctatatcttt tacctttaac tttagatttg
    11641 ttttcgcttt ctttaatgct ttttggttca tatctgtttt ggcaacataa ccgtgtttcg
    11701 ttcttccttt tggaatgact ttgattttga accccagaaa atctgatgaa tttttcttca
    11761 gatttactac ctttgatttc tctggactga tttctaattt cagcctttta ttaagaaaat
    11821 ctacggtact gtggtaaaat ctttgtgctt ctccataggt tgaacacatg attttaaaat
    11881 catcagcgta acgtacaata aaaccgcttt tcagtttggt atatttcttt gcgtattgaa
    11941 gccacccatt tttactgcgg ttcttcggct ggaatgtttc ccactgactg cttacccacc
    12001 agtctaattc attaaggact atgagggaga gcagtggact taacagaccg ccttgtggcg
    12061 ttcctttttc tgggacaccc tcgccgtcaa tttcggcttt cagcattttg ctgataatgc
    12121 aaatcaatct tttatcccga ataccaagtg tccagatttg ttttaatagt tttccatgat
    12181 tgacattatc gaaaaatcct ttaatgtcta cgtcaacaca gtaatgatat tttgataaat
    12241 ttatcagtgt cgttactctg ctgactgcat ggtgggcact tcgattcgca cggaatccat
    12301 aggaatgatt atggaacttt ggttcgcata tcggctctag gacttgcagg atacattgtt
    12361 gtaccagcct gtcccagata cacggaatcc ccaatggacg cattttatcg ctcccagctt
    12421 ttggaatgaa aacacgtctg actgatttgg ggcggtagtt gctaaggctt tcccgaacgg
    12481 tagcaacaat ctcagcattg ctgagttgtt ttatatcgtc tatcgtcatt ccatccgttc
    12541 cagccgtttt actgccactg tttgatttca gattacggta agctaaacaa atattttgtt
    12601 ccgaaccaat aatttcaagt aacttataaa agttattacc gttcttgctc tgtgcgtata
    12661 actcgtcata aatatgttgc atatcataat actcgttata gcggagcttg tttttcttta
    12721 gcatggtctg taccctcctt gtttgcagga gcaagtacat ctcttagtct tactcgaacc
    12781 cataccattt ttcttagtaa ttctattctt catatgacta gtggctatcc ctccaccgct
    12841 tgttatcacg gtttcatagg tactgtgcca ccactctcac tgacattaac acaccttata
    12901 tcattgctaa tctcacaata actttctgat gtgaacactt cacagacagt aaaatctcca
    12961 tgttgtcagc tttcaacgtt ccaaacacac tatctgtaca tatatcctta ggtttcctct
    13021 ataagcctgc taccttgata acgcctttaa cgttatatgg attttcataa atgcggagtc
    13081 ttactcaccc acagtagcga cactgtttgt gtcactggac atttctatcc agccccgata
    13141 tagacctgta cattcaagga ttcgtcagta gacctctcat ctacattcta accatagata
    13201 ttttatagac ttccggcata taggacacaa cgcccacctc tgggaatctt tcgtcagcat
    13261 tatctgttac ggtgtctgtc tgccgacttc accgagcttc tgacagagcc ttgtttactc
    13321 aagtttctgc cagtcagagt attaaagtag cctttcaggg cgttaccccg tcattcgact
    13381 attcgtgtgt ttagttctcc aacacacatt aaatattgat ttaatgaagt gctgtgccta
    13441 ctcttttcaa gtaggaacgt ttcgcacccc cttttggatc gacaatcaca gcttgaccac
    13501 cgaacaaaac agcatagtag actaagagat tgttactaaa ggatttacct ccaccaagag
    13561 aacccaggaa agcggcggcc agcgcattgg ttactgaacc cttgactcct tgtgaagcca
    13621 atgccggttt caggtaaaca ttacgacctg tatccacatt gtagccaaag taaatccctt
    13681 ctaattctcc tagcatctga gtagccccaa aaccaagccc cgccaaaaaa tcagacgtaa
    13741 catactgaat atagtcgttc atatatcgtt tacttaaggg taagaattct tcatgtagcc
    13801 ccatcatatc gccaaagggc cggactagtt ttacattggt atcatcataa aaatcgagca
    13861 cttcatcaca gcgtcgcttt agttcatcca ctgattcggc actcactcga accacataac
    13921 tcagtttgta catagattct tttgactgat ccaaggtggt ttccaattca tccacggaat
    13981 ccaaagcatc taagacattg gaattcgttt cattgtcaga ttggtaagca tgattgtcta
    14041 aatctttcag ttcttttttc ttattacgaa cggtagctaa agccttttta ttcgtcacaa
    14101 tctctacatt catacttgtg tcgattggga atgtaaattg ttgttgctgg taatagaaaa
    14161 gttctgagga aggaaattcc atctccccca caattgtatt aattgttaaa tacgctacgt
    14221 acgactcatg attctcatgt tccatccgta agcaccttgg tttttcttca attaaacaac
    14281 gatttggacg taataagtca taacgtttca ccagtgtttc tgattttagc tttttctttg
    14341 gtagctgaaa ttcgtattct tcaaacggta tccctttttc gccataaata tgttcaatca
    14401 aatacgtcag gtcactcggt gtgactcgcc tgactttaaa tcttcgagcc aatttgcttt
    14461 ccattaattt ttcaagctta gaatagcagc gaatttcttc attggataag gaaacaaaat
    14521 cgcccatcaa atgatgattc actccataga caaattcttg aagcccagaa aagaaagatt
    14581 tcttgagact ctttagattg acttcttcat ctgtcgcaat tagtttaaag ccaataaaaa
    14641 agcgataatc aatctgacta tcgccaatca tggatactaa tgcatctgtc tgtatatcaa
    14701 ttcgttgttt cgctacttct ttgagtctac cggtaatttc tttttttgat cgttcttgtg
    14761 tggcgcgaat actactttct gtggcaattt gtaacgcatg gatcttgcct tcacgatttt
    14821 gcgcaattag ttggcgaaag ttatcatgca cttgaaattt ctgttcgggt gacaaaaagg
    14881 agtaattgta aggcactaat tcatagtagg caaaacactc gccttcttgg ttaaagacga
    14941 gattattttc aatatactta atcggataca tagttcacac tccttacaag agtactgggt
    15001 tcatcccatc gtttcttctg ttctttgact ttctttcctg caaaagtcac cttaaatcgc
    15061 aagtgatagc tgataaagga tcgtaaaaat cccagtggtt tcttgccatc aaacgtcttt
    15121 tgcgacataa accaagtgac agccaccggt atgcctaagt attttaaaaa gacctcctct
    15181 atcatagata gtggaggcac atgggcaaat acgatgacaa acagtaaaga taaaacgaac
    15241 caagccattt ggttaaaagt cacaggaaaa ggcaactgaa aatcattgat tgcgtaaatg
    15301 actttttcaa ctgcccagat actggtatag ctttttattt ttttcatttt tcctcctaaa
    15361 aagggcggac ataataatat gtctcaccct ctagtgttat tctacaattt gtttaaaacc
    15421 atagcgtttc gctctatttg taaaagcatc ctcgccacct tccatcacag tacatattct
    15481 tggcttgata ttgggttcat ctaccgaccc gcactgatca gccgatattt cttttgaatc
    15541 tcccatgcta atcaaccatt ctgaatcacc attaactggg atatttgcat tatgagttac
    15601 aattaaaatt tgtctgtttt ccttttttgt aatgatactt cttacgatta agttattaat
    15661 taattgacta tccaaatcat cttcaggctg atccaatatg agtggtgaat ttccatacgc
    15721 caaaatcata gaaagaattg cagatgtctt ttgaccagca ctagcattcg ataaactaac
    15781 tttttttcca tttagtt
//
LOCUS       DAFFSV010000062        15783 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFFSV010000062 DAFFSV010000000
VERSION     DAFFSV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.062"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(27..725)
                     /locus_tag="LR110_001727"
     CDS             complement(27..725)
                     /locus_tag="LR110_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBL4822686.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="LR110_001728"
     CDS             complement(718..2070)
                     /locus_tag="LR110_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBL4822687.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="LR110_001729"
     CDS             complement(2293..3693)
                     /locus_tag="LR110_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBL4822688.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="LR110_001730"
     CDS             3854..4591
                     /locus_tag="LR110_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBL4822689.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="LR110_001731"
     CDS             4716..5195
                     /locus_tag="LR110_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBL4822690.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="LR110_001732"
     CDS             5192..5959
                     /locus_tag="LR110_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822691.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="LR110_001733"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="LR110_001733"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL4822692.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="LR110_001734"
     CDS             complement(6862..7464)
                     /locus_tag="LR110_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL4822693.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="LR110_001735"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="LR110_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="LR110_001736"
     CDS             8145..8729
                     /locus_tag="LR110_001736"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBL4822694.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="LR110_001737"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="LR110_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBL4822695.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="LR110_001738"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="LR110_001738"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBL4822696.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="LR110_001739"
     CDS             10630..10809
                     /locus_tag="LR110_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822697.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="LR110_001740"
     CDS             10831..11856
                     /locus_tag="LR110_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBL4822698.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="LR110_001741"
     CDS             12031..13275
                     /locus_tag="LR110_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBL4822699.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="LR110_001742"
     CDS             13428..14375
                     /locus_tag="LR110_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL4822700.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="LR110_001743"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="LR110_001743"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBL4822701.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="LR110_001744"
     CDS             complement(15277..15519)
                     /locus_tag="LR110_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822702.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="LR110_001745"
     tRNA            15640..15712
                     /locus_tag="LR110_001745"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
BASE COUNT         5361 a         2828 c         3201 g         4393 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aat
//
LOCUS       DAFFSV010000063        15781 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFFSV010000063 DAFFSV010000000
VERSION     DAFFSV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.063"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            123..605
                     /locus_tag="LR110_001746"
     CDS             123..605
                     /locus_tag="LR110_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822703.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            802..1692
                     /locus_tag="LR110_001747"
     CDS             802..1692
                     /locus_tag="LR110_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBL4822704.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2017..4488
                     /locus_tag="LR110_001748"
     CDS             2017..4488
                     /locus_tag="LR110_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL4822705.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(4591..6327)
                     /locus_tag="LR110_001749"
     CDS             complement(4591..6327)
                     /locus_tag="LR110_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBL4822706.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6324..7028)
                     /locus_tag="LR110_001750"
     CDS             complement(6324..7028)
                     /locus_tag="LR110_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4822707.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7159..7662
                     /locus_tag="LR110_001751"
     CDS             7159..7662
                     /locus_tag="LR110_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBL4822708.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7622..7959
                     /locus_tag="LR110_001752"
                     /pseudo
     CDS             7622..7959
                     /locus_tag="LR110_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
     gene            7980..9398
                     /gene="ffh"
                     /locus_tag="LR110_001753"
     CDS             7980..9398
                     /gene="ffh"
                     /locus_tag="LR110_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBL4822709.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9510..10088
                     /locus_tag="LR110_001754"
     CDS             9510..10088
                     /locus_tag="LR110_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBL4822710.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10092..10613
                     /locus_tag="LR110_001755"
     CDS             10092..10613
                     /locus_tag="LR110_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL4822711.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10692..11246)
                     /locus_tag="LR110_001756"
     CDS             complement(10692..11246)
                     /locus_tag="LR110_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL4822712.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11499..11774
                     /gene="rpsP"
                     /locus_tag="LR110_001757"
     CDS             11499..11774
                     /gene="rpsP"
                     /locus_tag="LR110_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBL4822713.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11786..12037
                     /locus_tag="LR110_001758"
     CDS             11786..12037
                     /locus_tag="LR110_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBL4822714.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12162..12953
                     /locus_tag="LR110_001759"
     CDS             12162..12953
                     /locus_tag="LR110_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822715.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13123..13644
                     /gene="rimM"
                     /locus_tag="LR110_001760"
     CDS             13123..13644
                     /gene="rimM"
                     /locus_tag="LR110_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBL4822716.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13634..14395
                     /gene="trmD"
                     /locus_tag="LR110_001761"
     CDS             13634..14395
                     /gene="trmD"
                     /locus_tag="LR110_001761"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBL4822717.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14531..14878
                     /gene="rplS"
                     /locus_tag="LR110_001762"
     CDS             14531..14878
                     /gene="rplS"
                     /locus_tag="LR110_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBL4822718.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(15010..15771)
                     /locus_tag="LR110_001763"
     CDS             complement(15010..15771)
                     /locus_tag="LR110_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL4822719.1"
                     /translation="MIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKNPIDEVVIDY
                     KRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGEAIALSKDDV
                     HITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNP
                     NGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKLAELGTPLYA
                     IQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         5189 a         2852 c         3211 g         4529 t
ORIGIN      
        1 ttctagatta aatgaataaa ctctcatgcc attatctcta aaaaaaaagt ttttccccct
       61 aattttttta gatagaaaat tgtatagtat gatgagagaa aaagtagttt gggggaacgt
      121 taatgagaag aaaagcagta atcgatcggc tttatttatt tttttggttt tttcctatcg
      181 ttttgctacc ttttacatgg gtaaatgctg ttgtacaagg tgcggagata ttaggcctga
      241 tccaaggaag cggatttgtg gtttctagga attttccagc tttgactgtt ttctatttag
      301 tgataagtgt aggtctctta ttaccaaaat ctctgatcaa tcactacaat tggcagctgc
      361 tttacttttc ttgcttatta gtatttacat ctatgttttc tttgctgcct cgcttgatta
      421 tcggccctaa tggtttgagc gagagtggat tgaaggattc agcttattcc tccatgtatt
      481 tcttgacgat caaaccatgg ttctatttag taattgcctc atttattctt gcaacatttt
      541 tttacttctg gacagaaatc aggataaaaa gtgaacgatc tgtatctata aaaaataact
      601 attgatggtg gatcatcagt aaaaaagcat atgagctgcg taaaatgcat gtttctcata
      661 tgcttttttt attattatgt aaagaatgcg aaaaacgttt acatgatttt tacataactt
      721 aactatattt ttacagcaac catacgaggg atttacagtc gactgataaa ctttagttgt
      781 aaataagaaa ggagcaataa aatgaaaaaa gtattgttta ctctaggtat gatgggaatt
      841 cttttagccg gatgcggctc aggatcagga aatacagatg gctctgccac acaaaataat
      901 tcaagtgact caaatgaaca agtaaaaatc gttgcggtag gttcgacagc attacagccg
      961 ttagtcgatg cagcacaaga aagctttgta caagaaaatc caaattatca aatctctgta
     1021 caaggaggag gaagcggaac tggtttgagc caagtcgaag ctggagcagt tactatcgga
     1081 aactctgatg tatttgcaga agaaagagac ggagttgatg cttcaaaatt agtggatcat
     1141 aaagtagcag tggtcgggat ggcgccaatc gtcaataaag atacagatgt caaagatatc
     1201 accaaacagg aattgattga tatctttaca ggaaaaatca ctaactggaa ggaagttggc
     1261 gggaaagatc aaaaaatcaa cgtcgtgaac cgtgcaaacg gcagcggtac ccgcgcgaca
     1321 ttcgaaaaat ggggactgga tggtgctacc cctgtgcagt cccaagaaca agattcatca
     1381 ggaacagttc ggcagctggt aagtcaaaca ccaggagcaa tcagctattt agctttctca
     1441 tatctcgatg attccacaca agcactaagt atcgacggtg tagaaccaaa agaagagaat
     1501 gtagcagata acagctgggg aatctggtca tatgaacata tgtacacgaa cggaaaacct
     1561 tcccctgaag tccaaaaatt cttagactac atgatgacag aagaaatcca agaaggacct
     1621 gtcaaagaat tgggctattt accaatcaca atgatggaag tagaacgtga tcacgaagga
     1681 aatatcaaat aactagcaga taaacatgcc acggtagtcg ttactattaa ggaaaaatca
     1741 actatgtttc ccacatatac atttttcctt taattgtgaa aaatagttcc tcatattttg
     1801 ccgttttttg agctaatacc gagggtcagc tgattaaaca ccgtttattc gaaaaaaaga
     1861 gctacgacat agaactgtcg tagctctttt tttgctatat gacaggaatg aaaggtgttt
     1921 gcaagcttaa tgtgaggaac actttccata tctgttctat actggatagg agaagtttgg
     1981 aaatatccag aaaacgaaat caatcgaggt agccacatga agaataaatg gataaatcga
     2041 agtaaaaaaa agaaagtaac acaaagatca tcagataaag tcatccgtct ttttccatcg
     2101 tataaagaag gattaacggc agatcaagtc aaggaacgaa tagaaaaagg tgcagcaaat
     2161 aattcagtcg atcccacatt caaaacaaat cagcaaatcg tattggaaaa catatttacg
     2221 tatttcaatt tgatctttct tatcttagcc attttgcttt gtttagtaga atcctataag
     2281 aacttgactt ttttaccagt tatcatagcc aatacaggaa tcgccatttt tcaggaaatt
     2341 cgatccaaaa agatcttgga cgaactaaat gttttaaata cagcaaaagt caatgtcata
     2401 cgggacggga tagaacaaac aatagatata gaagaccttg taatagatga cattgttatt
     2461 ttagagactg gacatcaaat tccagcagat gcagaggtcg tggaaggaaa actgcaagtt
     2521 aatgaggctt tattgacagg cgaagcagat gaaataacca aagaaatagg cgatccatta
     2581 atgtccggca gtttcattgt ttccggaaaa gcttacgccc gattagacaa agttggagca
     2641 gattcgtata tttcccagct gacagtcaaa gccaagacaa tgggagaagg agaacagtcg
     2701 gagatgattg cttctcttaa tcaattgatc aaatggattg ggatcatcat tattcctatc
     2761 ggtatcctgc ttttttctca aagttacttt tttaatggaa acacgatcaa agaaagtgtc
     2821 gttgcaatgg aagcagcatt gatcggtatg atacctgaag gactgtactt gctgacgacg
     2881 attgcattag cattaagtgc gactaaacta gcaaagcaaa gagttctttt acacaatatg
     2941 aaaagcattg agacattggc aagggtaaat gtcctttgtg tagataaaac aggaacgatc
     3001 acagaaaata aaatgtctgt tcaaaaagtg atcgtctcta aaaaacaaga aataatcagt
     3061 cagtcagatc aattggaagc atgcattgct gactatgcaa aagcgatggc aggagacaat
     3121 gcaacaatgg aagctgttaa agcatatttt acaaaaacca ctgatcaatc ctatcaccat
     3181 gttattcctt tttcttctgt ccaaaaattc agcagtgtag cgttggcaga caaggtgtat
     3241 gttttaggag cgcccgaaat ggttttgcgc gatcaattgc cggaatatgc ggaagagttt
     3301 gtttctttcg ctgaaaaagg ctatcgcgtg ttggtctttg gtgtgtacga tgggattctt
     3361 gaagaaccag tattgacaga agcagtcaca ccattaggct atattctgat tgctaatcct
     3421 atcagaaaag aagcaagagc cacatttgat tacttcaaaa aacagcaagt agccatcaaa
     3481 gtgatttctg gagataatcc gctcaccgta tccaatgtag ctgtccaagc agggatttca
     3541 catgctgatc agtacgttga tgcatctaaa ttatcagaag aagaatatga agcagctgtc
     3601 gaaaaatata cagtttttgg acgcgtcaag ccagaacaaa agaaaatctt cgttcaattg
     3661 ataaagcgta acaatacagt agctatgaca ggtgatggcg tgaatgatat tctagccatg
     3721 aaagaagcag actgcagtat agcgatggct tccggaaatg aagccgccat gcaggcctca
     3781 caagtggtct tacttgattc tgacttttca cgcatgccag aagttgttgc agaaggacgg
     3841 cgcgttgtga acaatattga gcgatcagcc agcttgtttt tagtgaagaa tattttctcc
     3901 tttttgctct cactgttttc agtgatcttt gctctgacct atcctttaga accatcacaa
     3961 atcacattga ttagtttgtt tactatcgga cttccatctt tcttgcttgc tctagaagaa
     4021 aacaaaaagc gaatcagagg gaaattcatc atgaatgtga tggaaaaagc tgtgccaggt
     4081 ggactaacag atatgatcgt agtcggtgcg ttggtgatct gcggggtgac tttgaatcta
     4141 aataaaacag atgtttctac agcatctacc atgcttttga ttgcagtagg gttcctcgtt
     4201 ctctataaaa tctgttcacc gctgaataaa ttcagaagtc agatcattct tttctgtgct
     4261 agcggtaact ttttttcggt catctttttg cataagctgt tttcaatcac cggtatctca
     4321 gctgtatcac ttttgctgtt gagcattcta tttttctcgg cagattcgat ttttaggcat
     4381 ttgaccacat tggtagaata tttgtttgaa cgtaaaaatg aaaaagagca aaagccgttt
     4441 agtcgcgtag ctaaaaaatt tctatcgata tttaaacgag aaaattgacg aaaagtatct
     4501 caaaaaagag taaaaaagaa caaccactcg tgtatctttc gaagcaggat gagtcatcca
     4561 actttaaaac gcacgagcag ttgctctttt ttacattttc ggaatggtaa cggtgaaagt
     4621 cgaacctgtt ccaagatgac tgtctacagt aatcgtacca ttaaggagtt gcacgtagtc
     4681 ttttacaatc gctaacccta atccggtccc gcctgaatgt cggcttcgcg ccttgtctac
     4741 gcggtaaaaa cgctcaaaga tccgttcctg atcctcaatg tcgataccta taccaaagtc
     4801 ttggacagaa aacgatagat catctgttgc ctgatagcgg ataataatct cgctgtcgct
     4861 ttttgaatat tggacagcat tttcaatcag atttttgata attggataaa ggatatctgt
     4921 tttagtagta aatggttcgt tctttccaat caatcgaatc gtcaaccgtt tcttttcgat
     4981 gattggctga taattttgaa cgatttgctg gaaataaggt tctgtataaa ttgtctggat
     5041 ctcgtaagaa ttctcgccat cttttgacag ctggatgatt tcccgaatca gtttatctaa
     5101 ccggatcgca tctttttgca tgatctcaag aaaagaagtc agagtttgcg ggtcatcttt
     5161 tgcaccatcc agtaaagtct cggtaaaacc aatcagtgaa gtcacaggag tttttaattc
     5221 gtgagagacg ttgccaacga aatccttttg tagcttttct aaacgtcgca ctttcgttaa
     5281 gtcatacgcc acccctagaa tctgtccggt accttctgaa ttagtaaagt agcgcaaact
     5341 caagtctaat gtctgccgac cttctgtaag ggtgatctcc tgatggatca gcggatgctc
     5401 aggagtcact tgatgaatca gctgaatgag ttttggctct tgaatgactt ctgtgtaccg
     5461 ctgttcaggt tgatagctta ccacgtttaa atgtagctgc atctttggat taagcagtag
     5521 taaacgagaa tcagcatcat caatcaggaa tacgccaatc atcagttcgt tcagtaacgt
     5581 atataattgc tcttctgtgg tagtacacgc ccgatacatc ttactcattt gttcgctcaa
     5641 cgcattaatc gtctgataca attcttccca ttgatcgggt gtttgcatga ttacttccgc
     5701 ctgattagga ttttgcacca ttttttttag tacgggcagc acggttttca atggctggtt
     5761 tttttgatag atcagataat agatcattcc tgcaagaaca aggaagaaag acagaaagaa
     5821 gaaaaagacc caacggcgaa aactctcagt tctcggtaaa aaaccgcttg tcggttctgc
     5881 gatacgtaaa atggcttcta actggccact ttttttgact ggtaatgcga catataaaag
     5941 atcctcattc aaagtagtac ttttgcgcaa agcgctacct aggttcccac cattcaatac
     6001 ggctttgatt tcgggacggg agtttcgctg ttcattcaat gtttcattgt ttgtgtcaaa
     6061 taagatcttt ccagtagcat ccattaaagt aatccgttcg ttcgactgat ggacgaattc
     6121 ttccaaagca gttttgtttt gcgctgcttg gaggttttcg acatctaatt gccggatcag
     6181 cagttgtgct ttttttgtca ggtagctttc ttgctgttcc acgatctgct gttgaaaata
     6241 atgggaaatc aattgccagc caccaaaaaa caaactaact aaaagaataa gaaagaagag
     6301 acgttcataa atttttttcc atttcatcgt tttggttcct gaaacttata gccaaatcct
     6361 ctaatagtca ccaagtactt tggatgtttt ggatctcgct cgattttttc tcgcaaatga
     6421 ctaacgtgta catctacgat tcggctttgt cctgcgaagt caaaattcca aatgcggtct
     6481 agcaatgtgt ctcggtcgat cacacggtct tttcgtttca tgaaataaac aagtaattca
     6541 aattctttcg gtgttagttc aatcagatgg tcgtctacgg tcacttgata attggtcaaa
     6601 tcagcagtga tttgtccaat cgatagatat tcaggctcag cttcatcttt tgattgctta
     6661 cgaggttcaa tccgtctgaa tattgctttc atacgggcta atacttcgcg aggactaaat
     6721 ggtttggtca aatagtcgtc agcacctatt tctaggccaa tgatccgatc cacttggtca
     6781 tctttagccg tcagcatcaa aatcggtgtt tcattttttt cttgacgcag ccgctgagtg
     6841 atctccatgc catccatcgt tggaagcatc acatctaaaa tgatgaaatc aaatgattgt
     6901 tctaatgcta attctaatcc ttcgcctcca tctgtggcac tggttacctt gtagccttct
     6961 ttttccaaat tgaacgtcaa gagcgttaca atagaaggtt catcatcaac aactagtatt
     7021 tttttcattc aaatctcctt taaataggtt tcattctgtt tatattttag catagaatca
     7081 gtacaaaggg ctattttaca aaagaaataa atggtataat aggaaggcac tttgctgaat
     7141 agaaaggaag agcagtcaat gattggaatc agtgcatgtt taggcggtct ggcctgtcgc
     7201 tatgatggac agaaaaaaga agtaccagaa ttgatggaac tagttgaaac tggagaagct
     7261 gtcatggtct gtccagaagt gatcggcgga ttaccaattc ctagagatcc cgctgaaatc
     7321 attggtggaa atggattcga tgtgtggaaa gatcaagcaa aagtatggac gacaagcggc
     7381 gaagatgtaa cggaagcgta caaaaatggc gcgatcaaag cctatcaaaa attgcaggaa
     7441 aaacacattg atctagtcat catgaaggaa aaaagccctt catgcggaac ccattcgatt
     7501 tatgacggaa gtttttccgg aataaaaaaa aatggtgccg gagtggctac tgcttatttt
     7561 attcagcaaa aaatgactgt gctctctgat gaagaatggc tgaaaactag aggtgagcag
     7621 aatggaaatc gaaaagacca atcgaatgaa cgcgctcttt gaattttatt cgaccttatt
     7681 gacagaaaaa caaatgaact acatggaatt atattatgca gatgattttt cattaggtga
     7741 gatcgcggaa gaatacgaag taagccgaca agcagtatat gacaatatca agcggacaag
     7801 caagatcttg gaggattatg aaaaaaactc catctttttt ctaattatat tgttcgagaa
     7861 caggtattgg aaaatatgac tgcatatatt acagataagt acccggaaga taaaaaacta
     7921 cttgaatatg tacagcaaat tcaagcaatc gaagaataaa taaaaggaat gaagaaaata
     7981 tggcatttga aagcttaact gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag
     8041 gaaaagtatc agaagctgac gtaaaagaaa tgatgcggga aatccgcctt gccttactag
     8101 aagccgatgt taacttgcaa gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg
     8161 taggggcaga agtgctggaa agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg
     8221 aagaattgac gatcacactt ggttctgaaa cagcagagtt aaataaatca ccaaaaatac
     8281 caactgttat catgatggct ggtcttcaag gggcaggtaa aacgaccttt actggtaaat
     8341 tagccaatta tttaaagaaa aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat
     8401 atcgaccagc tgcgatcgac caattgaaag tattaggtca acagctagac gttcctgtat
     8461 ttgatatggg aactgaagtc agcccggtag agatcgtccg tcaagggatg gagttagcaa
     8521 aagaaaagaa aaacgactat gtattgatcg atacggcagg acgtctgcat atcgatgaaa
     8581 cattgatgga tgagttgaaa caaatcaaag aattgacgca accaaatgag attttgcttg
     8641 ttgtcgatgc catgactggt caagatgccg tcaacgtagc tgatagcttc aaccagcaat
     8701 taggtattac aggggtcgtc atcactaaac ttgatgggga tactcgaggc ggggcagcac
     8761 tatcgatccg ctcagtgaca ggcgcaccaa tcaaattcat cggttctggt gaaaaattga
     8821 ctgacttaga agtattccac ccagatcgta tggctagccg tatccttggt atgggggata
     8881 tgctgacatt gattgaaaaa gcgcagcaag actacgacga gaaaaaagcg gaagaacttg
     8941 cacagaaaat gcgggaaaat tcattcgatt tcaatgactt tattgaacag cttgatcaag
     9001 tgatgggtat gggcccactg gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc
     9061 ctggaatcga aaacgtcaag gtagatccaa aagatgttga acgcaaaaaa gcgatggttt
     9121 attctatgac gccagcagaa agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc
     9181 gaattgctgc cggttctgga aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca
     9241 aggaatcaag aaaaatgatg cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg
     9301 accaaatgtt tggttcaggc gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga
     9361 tcaagaaaaa taagaagaaa aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta
     9421 cagactgaaa atgtctactg ccggagcttt tctatcagca atttttattc tttactaata
     9481 caattactct aaacgaagag gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa
     9541 gtatgcggca atatcatgat actgtctggg gtgttcctga atatgatgac caaaagctgt
     9601 tccgaaaatt gatgcttgat attaatcaag ccggattgag ctggcagacg attttaaata
     9661 aaagtgaagc attcgatgaa gcatatgatc gattcgatat caaaaaagtc gcaaattatg
     9721 gagaaacaaa aatcagtgaa ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa
     9781 tcgaagcagc cgtccaaaac gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt
     9841 tttcagagta tctttggtcg ttttctaatg gaaaaatcgt tgattcttct tatcattcac
     9901 aagaagaagt cccaacaaca agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa
     9961 gaggatttaa atttatcgga agcacaacaa tctatgcttt tttagaagct gtcgggatca
    10021 tcaatgatca ccttgacagt tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg
    10081 gcgaataaag catgcgaaaa atcatatttt atggtgctat aagtttagat ggctatttgg
    10141 caacgaaaac ggatgatctt caatggcttt ttgacactcc aacaggtaag caaactactt
    10201 atgaagcttt ttatgaaaca atcgatacga ccatcatggg cagaaaaact tatcaggaag
    10261 caaaaaaata tttggagacc gaagcaattt atccagagaa aaagaatttc gttttttcca
    10321 cccacgcaca attacaattg ccagatgctg aggtcgtaaa cgaagatcct gttgcttttc
    10381 taaaaaatct acaaatgaca gaaggaaaag atatctgggt tgtgggtggc ggtcgattga
    10441 tcaaaccgct attggaacat caaatgattg atgaatggta tatccagatt acccctgtat
    10501 tattagggga cgggattcct ttattccaaa aaggaacata tgaagcgcgt tttgaaatgc
    10561 tagacacgac acgattcggt gaatttatcg aattgcatta tgtacgaaag tagaagtaaa
    10621 taaaataaaa aaggcaacta tatgcagaaa tgtcctgtac atagttgcct tttttattac
    10681 cgttatgctt tttactctgt taatttgtta gaaaaatcta gatacgcatc taaaatcgtt
    10741 tggaagaaac ttaatgttcc ttcaacgata ttaccatttt catcaattag attcgcaacg
    10801 tttccgatat atgcttccgg ttgctgcaag gttggcacat tcaaaaagac aagtgattgg
    10861 cgtagatggt ggtttgcgcc aaagccgccg atcccacctg gtgatgcagt aacgactaaa
    10921 cctggtttat gatcccaaac actgtgtcca taaggtcttg agcctacatc taaagcattt
    10981 ttcaatactg ctggcacaga acggttgtat tcaggagtaa cgaaaacgac accgtctagt
    11041 cccctcactt cctcacgaaa acgagtccat tcagcaggag cctgttctgg agtttcgaga
    11101 tcctcattgt aaaatggcag atcatcgatt tttacaaata cactttcgta cccttcagga
    11161 agcaatttcg caaaagcttc tgctactttt ttgttgtaag aatcttttct taaactacca
    11221 ataaaaaatc caattttttt tgtcatacga tttttcctcc ttcaaactat ctataataat
    11281 cttacaaaag ataagtaact ggagcaaata aaaacactta ctgacaaaag ctacatagaa
    11341 gatagattac ttatttttaa caaaaaagaa gtctaaaatc ttctattttc taaaaaaaat
    11401 cgaatttaat caagctgtaa agaaaaaaag ctttacaggg taaaaaaaaa ctgttacact
    11461 acatcttgtg aataaaacaa tggaggtgga aataaataat ggcagttaaa atccgtttaa
    11521 aacgtatggg ttctaaaaag agtccgtttt accgtatcgt cgtagctgat tcacgttctc
    11581 ctcgtgatgg acgtttcatc gaaactgtag gtacttacaa tcctttgaaa gaccctgcag
    11641 aagtagtttt aaaagaagac ttagttcttg actggttatc aaaaggtgca caaccttctg
    11701 acactgtacg taacatcctt tcaaaagaag gcgttatgca aaaacaccac gaagctaaat
    11761 tctcaaagaa ataaggtgac cggatatgaa agatttaagc gacttagtct taacaatcgt
    11821 tcgtccgcta gtcacttatc ctgatcaagt caagttagac gtcgtagagt cgaaagactt
    11881 ttacgagtac aatctaacag tggctcctga agatattggc cgtatcattg gaaaacaagg
    11941 gcgtgttgct aaagcaatcc gcacaatcgt ttacggtgtt cggatcaatg caccgaaaaa
    12001 agtgcgtttg aatatcatcg atgataagga aaagtaaata aacgtgctgt actcgaattt
    12061 tcgagtgcag cttttttgtt ttttttatta attgaagaaa tgattatttg gcaaaacaag
    12121 caagaaagcg tatccttata ggaggaggga gtgaacagcc aatgagaaag aaaatgttat
    12181 ttattccatt tgctctattg ttaagcgctt gtagttcggt tactcaagaa tcagacaata
    12241 cacatctatc aagcagtata tctgaaactg ttaccagtcc agaaaaaagt agtaccaaaa
    12301 cggcttccac cactgaacaa acaatggcta cctcaaataa tgagaaaaag actgctttag
    12361 accagttgaa agaacaacag ccaaatgttc ccatgcctct agatgtacca gtctcttcag
    12421 gctatctcaa tatcgcagcg acacatacaa agcaaggtta ttctatttta tattacagaa
    12481 cagatcgtcc cctcgggttg aatgctgacg aattgaatca agaaacgcct attgcaacct
    12541 atctatatca atatgggttt gcttctagtc aagaaacaat ccaagttttg cagccatttg
    12601 agatcgacac aaatggtcag caagtggact taggttctcg aatcacaggg tatcagcaag
    12661 gtgcagcagg ttcaagtttt cttgaatggc aagaaggaaa ctggcgtatt cgaatccgtg
    12721 ggaataatat agaaggccaa gatcccttac tattagctaa agaaatcgta gcttatttgg
    12781 aagaaaacag tctaccagca cctgaacaat ttggtaaaat tactgttgat atgggtgata
    12841 cgacgaatcg agccgtagaa gtaagctggc aagaaccgaa aaatgcctat acgatcactc
    12901 atcaagatcc tatgtctgcg ttgaagatgg cagtttctat gaagaggttg tgaaatcagc
    12961 gcgctgatct gagcccatca tttaactcca agtcacagga agtaatcaaa aatagcacgc
    13021 cgcatttttg cataattttg gcttaatacc ttaaaagaaa actagaattg cctcttcaca
    13081 catgctaaaa taaaacagac aaaaaagaaa tggggatata agttgactga atacttgaat
    13141 gtaggaaaaa tcgtaaacac acaaggtatc aaaggcgaag tccgtgttat ctcaacaacg
    13201 gatttcccag aagaacgtta caaaaaagga acggtgctaa cactattcca agacggaaaa
    13261 gcacctgtgg aattaacagt caaaagtcat cgcaaacata aaaattttga cttattgagt
    13321 tttgaaggac atccttctat caatgatgtg gaaaaatatc gggatggcat attaaaagtg
    13381 tcaaaagaca actctgtaga tctagctgaa aatgaattct attaccatga gatcatcggt
    13441 ctgcatgtat tagaagatgg aactgagtta ggtaaagtaa aagaaatctt gtcaccaggt
    13501 gccaatgatg tttgggtcgt tcaacgtccg aaaaaagctg atatattgct tccatatatc
    13561 gcttctgtcg tcaaagaagt cgatttagaa gctggagtgg tccgtgtaga aattccagaa
    13621 ggactgattg acgatgaaga ttgatgtatt gactttattt ccaagaatgt ttgaaggtcc
    13681 tatgggggaa tccatcatcg gtaaggctgt agataaagga ttattagata tcaatatctc
    13741 taacttccgc gattattcag ataacaaaca tcagacagta gatgattatc catacggcgg
    13801 tggagcaggt atgctgctca aagtccagcc catctatgat aacatccaag ccattgaaaa
    13861 agctgcaccg gatgtcaaaa aaagagtaat tcttcttgac ccagctggta aacgattcga
    13921 tcagaaaatg gcagaagaat tttcacaaga agaacactta gtattcattt gtggtcatta
    13981 tgagggatat gatgaacgta tccgcacatt agtgacagat gaagtttcct taggagatta
    14041 tgtccttact gggggagaat taggtgcgat ggtcatgatc gatgcaacag ttcggctttt
    14101 gccagatgtg ttagggaatc agacttcagc tcaaacggat tcttattcaa ccggtttatt
    14161 ggaacatccc caatatactc gcccggcaga atataaaggg atgaaagtac cagaagtatt
    14221 gacaaatgga aatcataaat tgattgaaga atggcaatta aaagaatcac taagaaggac
    14281 ctatcaacgt cggcctgatc ttttggaaac acttgaatgg acacctcaaa tgacgaaatt
    14341 tttagaagaa atcaagaaag aagaacaaga aaaaacagca gatgaagcca agtgactttt
    14401 tctaaaatct tctttacagt aggttgataa tatgttagta ttttatggtg agtgcaaaag
    14461 cacttaacta ttacgatatt ccgctgtgag aaaagctcat aagaatattt ggaataagga
    14521 gaagaaaacc atgaatccat taatcgaaga attaacaaaa gaacaattac gttctgacat
    14581 cccagcgttc cgccctggtg acactgtgcg tgttcacgca aaagttgttg aaggtactcg
    14641 tgaacgtatc cagttatttg aaggtgttgt tatcaaacgc cgcggtgctg gaatcagcga
    14701 aacttataca gtacgtaaag tttctaacgg agtgggcgtt gaacgtacat tcccattgca
    14761 tacaccacgt gttgcaaaaa tcgaagtagt tcgctacggt aaagtacgtc gtgcaaaatt
    14821 gtactaccta cgtgcattac acggaaaagc tgctcgtatc aaagaaatcc gtcgttaatc
    14881 atcgttatca gacagagaaa gaacccttgg tattcaaggg ttcttttttt attatctgtt
    14941 tttttatatt aacgatttct atttggtaca tttttgatta ataaaaaggg gcaaaaaagg
    15001 ggcaacaagt tacagttttt ctatatcttc tttcaatttt tcttttactc cttttgtcac
    15061 atgcaaataa attttttcag tgatatcact attttcgtga ccaacgcgat cctgaatggc
    15121 atacagagga gttcctagtt ccgctaattt tgaaatatgg gtatgtctaa aaatatgtga
    15181 actaagtttt ttatcaatct tcatgtcagc tttatgactt ctcaaatagg tgttaattgc
    15241 tgttagttgg aaaggagttc ctttcgttgt ttgaaataga aattgaccat ttggatttaa
    15301 ttctagaagc tcattgtaaa tagctatcgc tttttttggt aaatcaattt ctcgcattcc
    15361 ggcagctgtt tttgtagaat cagatttttt catatcagct attgaacgtt ctctatacat
    15421 catcgtccca tttataacta ctgatgcatt attattggta atgtgtacat catctttact
    15481 taatgcgatt gcttcaccag acctcatccc agtcaaatat agccactgga aaaggagaga
    15541 atatctttta ttgtgtgaag ttgtgaaatc tactagtcta ttatattcat catcttctaa
    15601 aaatttatct ttaattttta tcgtttttga ctctctttta taatcaataa ctacttcatc
    15661 gataggattt ttttctacat aacctttttt catagcatat gaaaaaagaa gattcaattt
    15721 ggatttaatt acactgacgt acttatttga caagtcatct ttatatatca tgtcttcaaa
    15781 a
//
LOCUS       DAFFSV010000064        15076 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFFSV010000064 DAFFSV010000000
VERSION     DAFFSV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.064"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="LR110_001764"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="LR110_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL4822720.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="LR110_001765"
     CDS             complement(322..891)
                     /locus_tag="LR110_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBL4822721.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="LR110_001766"
     CDS             1077..1979
                     /locus_tag="LR110_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL4822722.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="LR110_001767"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="LR110_001767"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBL4822723.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="LR110_001768"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="LR110_001768"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBL4822724.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="LR110_001769"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="LR110_001769"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBL4822725.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKIDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="LR110_001770"
     CDS             8547..8732
                     /locus_tag="LR110_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBL4822726.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="LR110_001771"
     CDS             complement(8794..9990)
                     /locus_tag="LR110_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL4822727.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="LR110_001772"
     CDS             complement(10160..11596)
                     /locus_tag="LR110_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL4822728.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="LR110_001773"
     CDS             11889..12698
                     /locus_tag="LR110_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4822729.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..15037)
                     /locus_tag="LR110_001774"
     CDS             complement(12884..15037)
                     /locus_tag="LR110_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBL4822730.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4399 a         3195 c         2666 g         4816 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtca attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac
    15061 attcatgctt cattgt
//
LOCUS       DAFFSV010000065        14864 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFFSV010000065 DAFFSV010000000
VERSION     DAFFSV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.065"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            64..705
                     /locus_tag="LR110_001775"
     CDS             64..705
                     /locus_tag="LR110_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL4822731.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            748..1440
                     /locus_tag="LR110_001776"
     CDS             748..1440
                     /locus_tag="LR110_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBL4822732.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            1437..2339
                     /locus_tag="LR110_001777"
     CDS             1437..2339
                     /locus_tag="LR110_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL4822733.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2497..4203)
                     /gene="efbA"
                     /locus_tag="LR110_001778"
     CDS             complement(2497..4203)
                     /gene="efbA"
                     /locus_tag="LR110_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBL4822734.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            4393..5016
                     /locus_tag="LR110_001779"
     CDS             4393..5016
                     /locus_tag="LR110_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822735.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            5466..6515
                     /locus_tag="LR110_001780"
     CDS             5466..6515
                     /locus_tag="LR110_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4822736.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            6512..7393
                     /locus_tag="LR110_001781"
     CDS             6512..7393
                     /locus_tag="LR110_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822737.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            7396..8166
                     /locus_tag="LR110_001782"
     CDS             7396..8166
                     /locus_tag="LR110_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4822738.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            8445..9299
                     /gene="aroE"
                     /locus_tag="LR110_001783"
     CDS             8445..9299
                     /gene="aroE"
                     /locus_tag="LR110_001783"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBL4822739.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            9477..10640
                     /locus_tag="LR110_001784"
     CDS             9477..10640
                     /locus_tag="LR110_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL4822740.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            10757..11167
                     /locus_tag="LR110_001785"
     CDS             10757..11167
                     /locus_tag="LR110_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL4822741.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            11341..13560
                     /gene="topB"
                     /locus_tag="LR110_001786"
     CDS             11341..13560
                     /gene="topB"
                     /locus_tag="LR110_001786"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL4822742.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLINSLEKEAIYEGFKNLQPGKNYFPKY
                     KEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIKHFK
                     KETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEVTKK
                     EKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITENEH
                     QYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFAKLS
                     KLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILRIKS
                     KKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEAQEI
                     LKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSAEMT
                     ARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIKAME
                     KKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSKGKT
                     SKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENEVE"
     gene            13731..14015
                     /locus_tag="LR110_001787"
     CDS             13731..14015
                     /locus_tag="LR110_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822743.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            14175..14411
                     /locus_tag="LR110_001788"
     CDS             14175..14411
                     /locus_tag="LR110_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL4822744.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            14408..14806
                     /locus_tag="LR110_001789"
     CDS             14408..14806
                     /locus_tag="LR110_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBL4822745.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         4996 a         2522 c         3159 g         4187 t
ORIGIN      
        1 cgaatatcag ttatttaatg atatgatatt cggaaaaaga aatctcaaag ggaggtaact
       61 tttatggggt tgaatgaatt tattttagga ttgaacaatt tagagacgat cacaagggca
      121 cctggatttt ttaaatatac agagcataca gttgcagcac attcgtatcg tgtagcatct
      181 attgcgcaag ttctaggaga cattgaagaa gcgaatggac aaccgatcga ttggaaactg
      241 ctctatgaaa aatcattgaa tcatgactac acagagcgtt ttattggtga tatcaaaacg
      301 ccggtgaaat acgcaagcaa agagttaaga gggatgctgc aaacagttga agaaaagatg
      361 acggacgaat tcatcaacca agagattcca gctgagttcc aagaagttta tcgccgtcgg
      421 ttgttcgaag gaaaagatga aaccgtagaa ggagaactat tagccatttc agataaggtt
      481 gaccttcttt atgaatcttt tgatgaaatc atcaaaaata acccggaaaa agtttataag
      541 gagatgttca ttgaggcagt gactacgatt aaaagctttg aacatcggcc ttctgttcag
      601 tatttcttta aagagatatt tccggaacta ttgaaccagg aattttatgg aaaagaggcg
      661 tttttagaaa ccgtctcaac gattgtaaag aaagaagaaa aataaaaatg atatgagatg
      721 cagggggaag tatagttgaa aaagaatatg gatgtcgaat ggggctattc tggagacaaa
      781 ggtccggaaa attgggcgag tttatgtgat tggtttgcga gaggtgcgga atttccattg
      841 cagtcgccta ttcatttgac caagggggaa gaaaccaaaa tagaaggaga cacgctttcc
      901 tttcattatc aaaagcaacg gtttactgac aaggaattca aaaacacgat tcatttagta
      961 ccttttgacc aactcagtta tgtagagttc aagaaagaac gctattattt gacagatatt
     1021 catttccact tgccttctga acatatcatc aacggaaaac aggaagatat tgaatttcat
     1081 tttgttcata tgaacagcaa aaaagagaat ctagttgtgg gtgtcatgta ccagctaatg
     1141 gtacaggaag gttggctgta taatcaagaa aatggggaaa gttggaactt agaaaaacat
     1201 gagcattggg taaatccgga tgtgttcttt ccggagcaaa aaagccattt ccattatatt
     1261 ggctcattga caactccgcc taccagcggg ccaatccaat ggtttgtaat ggatcatgta
     1321 ggaaaactga accaagagtt tcttctcccc ttcgatggtc agtatgctcg accgaacaac
     1381 cgaccaatcc agcccttgaa tggaagagaa atttattatt tcgaggagtt tgagcaatga
     1441 atattcagca gatgaaatac gtggtcgctg tagcaaacaa tggtagtttc agagaagcgg
     1501 caaagaaaat gtttgtctcc cagccaagtc tttcagctgg tatcaaagaa ttagaaaccg
     1561 agttagggat cacgctgttt actcgaacga atcgaggggc atatttaaca gaagaagggc
     1621 aagacttctt aaatcgtgcc gagaaaatat tggtccagct tgatacatta gaaactcact
     1681 atctaacgag tgaaaagaga gacaattttt cgatcgctgc ccagcattat gatttcttag
     1741 gtcctttgac cgcacagcta ctcgaggaat tcaaagacga gatcaaacaa tttcggatca
     1801 tggagacgac aacagcgaga gtgatcgaag aagtcaaaga gcagcacagt gagatcggca
     1861 ttatttatat gaatgagcat aatcaagctg gtatcagaag atatttacaa caaggagagc
     1921 taagtggccg cagcattgga aaattcaaaa cgcatatttt tttaaggcaa ggacatccat
     1981 tagctgaaca cgcagagatc accaaagaag aattgttatc ttatcctcaa gttcgtttta
     2041 cacaggatgg aaataatttc ccgtattttt atgaagacct tatcgagatc cctgatcaag
     2101 aaacagtgat ttatacgagt gatcgaggta ctttgatgaa tatcgttctt gaaactgatg
     2161 cttacgcttc aggatctggt attgtgattg gagggattcg cgagcacttg cggttaatcc
     2221 ctttggcaga aggcgaacta aatgaatttt atatcattta ttcggcgaaa cgtccgctca
     2281 gtgaaatcgc tcaaagattt atcaaagagc ttactcggtt actggaccag cagagctaag
     2341 aaaagcagat gagaacgctg tttattgaac accgttgatc aaaaaagagg ggttgtgaca
     2401 gaattttgtc acaactcctc tttttgtagc aaacaattta taactaattg ctgttgtggg
     2461 aaagtacgca aatatgattc tagaaacact cattgattac ctgttttgtt tcatagctgt
     2521 aattttttct tcttctggtg tcacgataat cgtcttttgg ttttcataaa taacataacc
     2581 aggcttggcg ccatttggtt ttcggatatg ctttacctgt acaagatcaa ccggcacttg
     2641 ggcagagtat cggtattttg aaaaataggc agctaactcg gcagcttctg taattgtttc
     2701 atctgagggc ttgtcacttt taatgatgac gtgcgaaccg ggtatatttt ttgcatgaag
     2761 ccaatagtcg gtttttttag ctgtcttcat tgtcagctgg tcattttgaa gattgttttt
     2821 accaacaaga atgagcgttc cgtcagaaga aaagtattga tcaggctgag atggtttctt
     2881 acgtttttgt ttttttaagt tcttcttttt caggtatcct tctgaagtca attcttcttt
     2941 aattgcttcg atatccattg ggccggcaat ttccagttga gataagacag attcaagata
     3001 ttggatttca tctttggctt cttggatttg ttcgccaatc agcttaacag cgttcttcag
     3061 cttttggtat cgatggaaat atttctgtgc attttgattt ggtgtcaatg ccggatctag
     3121 tgctattttg attggccgat cttcttcata ataattaggt aaaacaactt cttttgcgcc
     3181 gcgtggtact tgtgtcagaa aagtagtaag tagttcgcca tttctacggt aattctcagc
     3241 gttttcggat tctttgagtg tttgttcacg ttttttcaat ttatttttat ttcgttttag
     3301 ttcattttcg atttttcgaa tcagttctcc gccttgttgt tttgcccgat ctttctctgc
     3361 tttttcgtga taatagctat ctaataattg actgagtgtt ggataagctg ttacagcaga
     3421 tgcctgttcg gacaatacct gataagcaat cggtgtgaaa aaatctttat tttccgtttc
     3481 gtaaaaagta ggaataggct gatggctgat ggctgagaaa aattcgtccc atacaaccat
     3541 cttttcatta ggacgttcca gtaatctttt ggtcagctct tgagcgatat cgaaaccgat
     3601 tccctgaaac tgacgttgga ttgcttttgg atctagttca gtttgggaaa gccgctgaaa
     3661 gattttttct ttctcggagc taaatgggtt caagacctct tgttttggcg gagcaacata
     3721 ttctacacca ggaagcaaag aacgataagt gttttgagag cttccaatgt gtttgatcgc
     3781 atccagtatt tttccagttt ctcgattaac taagacgatc gtactatgac gtcccattaa
     3841 ttcaacgatc aatatgatat tttgtagatc acccaactcg tttcgtttag caaagtggaa
     3901 atggatcact cgatcatttt cgatttgttc gatgtcttct aaaatagcgc catccaaata
     3961 ttttcgaagc atcatcacga agttaggcgg attatcagga ttctgataat cgatttgggt
     4021 aatctgtaca cgagcataac ttgggtgcgc agataataat aatcgatggt ttttgccgcg
     4081 tgaacgaatc accagcacga tttcattttc ataaggctga tggattttcg agatccggcc
     4141 agatagtaac gtttcacgca attcgttgac catcgcgtgg gtaaatacac catcaaagga
     4201 catggttcat tcctcatttc ttttcataag tatattcatt ataacgaaaa aaagaagaaa
     4261 aaccaatttg gccgaatagt aaagcaaaag tctagaaaaa tcttggacta attacataaa
     4321 aaacagtgtt tctatcgttg aaatccaaat ttttaggtat aataaaaaat aggagaaaaa
     4381 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
     4441 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
     4501 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
     4561 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
     4621 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
     4681 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
     4741 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
     4801 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
     4861 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
     4921 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
     4981 ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat gaaacggtgg
     5041 gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc aaaataacaa
     5101 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
     5161 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
     5221 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
     5281 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt gactgccgca
     5341 tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc cattgtcttt
     5401 ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt tgcaggagga
     5461 aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt tatttagcag
     5521 gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg aaccagacaa
     5581 aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta gatgagattt
     5641 atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa aatcttgaga
     5701 tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt caacagctag
     5761 tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa tcattagcaa
     5821 acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg ggggctggat
     5881 tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat cagccgcctg
     5941 ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa gtcggaatct
     6001 tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa gaagctgcaa
     6061 aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag attgcacaaa
     6121 cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat aataccattg
     6181 ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc gtcattccat
     6241 ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat caatatcaat
     6301 tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa aaaccggaaa
     6361 cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct caagctgatc
     6421 gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt acagatgact
     6481 cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt cacaaggcat
     6541 gctttgggca atattaggat tagggatttt catgacttat cggatcttag actttcctga
     6601 tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag ctattactca
     6661 tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga tagcaggact
     6721 cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag ggattttagt
     6781 gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc tttctttatt
     6841 gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt actttgacac
     6901 agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat tcttcttcaa
     6961 tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg ctcggtcgtt
     7021 agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg gtgtcattgc
     7081 attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata aagggattgg
     7141 tgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc aagaattgac
     7201 gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc tattgatttt
     7261 agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg ctatcatctt
     7321 agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa cgggttttga
     7381 gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa agtcgtaaac
     7441 aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat cagagaaggg
     7501 gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt caatacgatc
     7561 gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct gatcacaaaa
     7621 gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc caagatggga
     7681 acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag aggtcaaaaa
     7741 cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag actatgtcaa
     7801 gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt atctggtggt
     7861 caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt actattatta
     7921 gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg attaacaaat
     7981 cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat ggaagacgcc
     8041 ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca agatatttct
     8101 gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga agaagtagtg
     8161 gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca ttgacatctt
     8221 cctttgattc atcctatact taaaacaata aacaattaaa catatttggg tgttttgtag
     8281 gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg acaagtaaac
     8341 gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc gtatcaaacg
     8401 ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt tctggacaaa
     8461 ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca ttgatgcaca
     8521 atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa gtaaatccaa
     8581 tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt gtaaatcttt
     8641 cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag gaagcgcaat
     8701 tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc tataatactg
     8761 atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa aagaaaaaaa
     8821 tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagca gcgttagacg
     8881 gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa gtaaaagaaa
     8941 cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac tttgccgata
     9001 cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca acagatatcg
     9061 ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg catgagaatc
     9121 tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa gaagcaaaaa
     9181 aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga gcaatcgcgt
     9241 ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg tttgattaaa
     9301 aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa aagtaatcct
     9361 ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa tgataggctt
     9421 ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga accacaatga
     9481 gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag cgggatacgg
     9541 ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag gaagatgcta
     9601 cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac gagcaacaga
     9661 tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt ttcaatcggc
     9721 aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa atcattctat
     9781 cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt aaatatgtag
     9841 ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg caaaaagcga
     9901 cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa gatgccgggc
     9961 gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg aaactgattt
    10021 cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag atcaattcag
    10081 cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat tttggaatcg
    10141 attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc gatgccttat
    10201 tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt gtagatatta
    10261 aaaaaggggg ccagaagatg gatggacatg ttcttttgca gtacgcacgt ttccgaatgg
    10321 atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac gctattttca
    10381 gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt aaagctttag
    10441 gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt tcgattgcac
    10501 gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg aattatgggc
    10561 aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa tctgctatta
    10621 cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca aggcttttgt
    10681 catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga tttaaccaag
    10741 agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc gtgaagccat
    10801 cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat acggtgaaat
    10861 gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca cgaatgcacg
    10921 gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa ttggtcctga
    10981 attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag aagaaacgaa
    11041 gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg atgctgttcc
    11101 gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc agattaacca
    11161 agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg gatcatcgtt
    11221 tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt tattgccaga
    11281 tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg aggtcagaag
    11341 atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc cgaaaccttc
    11401 caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt tgctggtgaa
    11461 gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg agcttatgaa
    11521 gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac cttccgttac
    11581 gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt gcagtcagcc
    11641 gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc gtggtcgatt
    11701 attcgacaag caaacgcatt ttctaaggaa aagaactatt tgatcaattc tcttgaaaaa
    11761 gaagcaatct atgaaggttt taagaatctc caacctggaa agaactattt tccaaaatat
    11821 aaagaagcgc aagcaaggca gaacgctgat tggctgattg gaatgaatgg aagtccatta
    11881 tatagcttgc tacttcaacg aaaagggatt gatggcagtt tttctcttgg tcgtgtacaa
    11941 acaccgacac tctatatgat ttatcagctg caagaagaaa tcaaacactt caaaaaagaa
    12001 acgtactttg aaggagaagg tttaattacg acaaacaaag ggcagttttc tggaaaaatc
    12061 gtaccaaaaa aagcatttaa aacccaagaa gagttaacga aggaaataga aaaacttgga
    12121 gcgcatttag gtaagcaaaa cggacggatt cttgaagtta cgaaaaagga aaagaggttg
    12181 catagtccac gtttattttc gctatctagt ttgcagacaa agatgaatca gctaatgaaa
    12241 gcaagtgcca aagctacact tgatgctgct caaggattgt acgaaagcaa gtttctaagc
    12301 tatccaagaa cggattcctt ttacattaca gaaaatgagc atcagtatct ggttaaacat
    12361 ctgcaaaatt ataaaaaatt tttaggaata gaagaagtac agacaactga gatcaaagca
    12421 aagaaacgat atgtcgatgc gaaaaaagtt caagaacatc atgcaattat tccgacaaag
    12481 acgattccga ctcctgcacg ctttgcgaag ctttccaaat tacagcaagc aatctatttg
    12541 caagttttgc gcactactgt ggctatgttt gctgcagatt atatctacga agagacgacc
    12601 gtaatgacgg gcgtacagca attaaagcta aaaagcatcg gaaagatccc attacagcaa
    12661 ggttggcagg aaatcttacg catcaagtca aaaaagaaag aagctcagac tcttcctcct
    12721 atcgtagaaa atgaagaagc agaagtagaa ctgaaaagta tggagaaaga aacacaacct
    12781 ccaaaaccct ataccgaagg aacgttgatc acagcaatga aaactgcggg aaaaacgctg
    12841 gataatgatg aagcgcaaga aattctcaaa gaagtagaag gaatagggac agaagctaca
    12901 cgagccaata tcattgaaaa tcttaagcag aagcattaca tcgaagtcaa taaaaacgaa
    12961 ataactgtta cgccaaaagg aatcactctt tgtaaggcag tagccaatga accattactg
    13021 acgagcgcag aaatgactgc gcgctgggaa gggtacttga gaaaaattgg taaacaagag
    13081 gggacaccat ctgttttttt agagaatatc aaaaaattca tcctacattt attggcagaa
    13141 gttcctaatc aaatcgacaa ggtagacttt tcagatgaga ttagcgggac acgtcagata
    13201 aaagcgatgg agaagaaaaa cgatcaatta ggaagatgtc caaaatgtaa aaagggaata
    13261 gtcatgcttt atcctaaaat cgctacatgc ttgaatccgg aatgcgactt caaattgtgg
    13321 ccaacagtag ctaaaaagaa gctgactaaa acgaatctca gagatttgct ttcaaaagga
    13381 aagacaagta aagttgtcaa aggttttact ggtaaaaaag ggaaattcga tgcagtttta
    13441 gtattaaaag aagatatgac aatcggtttt tcttttccat ggatcgaaaa cagtggaaca
    13501 aaagcagttg atggaaacaa agaaacatca agaaaaaata atgaaaacga agtagagtaa
    13561 atgaaaacaa tgaaatagcg gaatatttat tccgaaaagt gagaatataa ataccaataa
    13621 aactaaaaaa tatcagtgaa tcaagcaaaa acgttggggt aggctgattt tatatagata
    13681 gcaaggagtt tttttatttg aaaaaagtgc attcattttt aaattatctt atgggagcaa
    13741 tcacgattgt ggctgtgtac caagcatttc taaagtatcc tgattacggg acgaagttgt
    13801 tgtcattgac gcacaatcaa acagcatatt caccaacgtt gattttatcg actttgactg
    13861 caggagctaa aagcgctact tatttttttc ataaaacgac aagcatgcta aagcgaaaga
    13921 aacagcgaaa acaattgttg gtggtggtag cggctatgat cattatttat tttctaccag
    13981 ttattacaca agtatttcat caatattttg gttaagaata cgagagtttg tgacaaaagt
    14041 catgtcacaa acttttttct ttgtcatttt ttcgtaagaa aaagcaaaat aaaagtatat
    14101 acattgatta aaagtatata cttggatata atgtacttac tatagaatga gaatggtata
    14161 gagggtggcg aaatatgaaa aaaatgaaaa agtataaaat tagaaaatct ggcaatagcg
    14221 atgtaacaac tatcccacca gaagtaaaag aattcatggg tgtgcagaca ggtgacgcaa
    14281 tcagttatgt ttttcaatct gatggttctg tacgaatgat caaagcacaa gaagaacctg
    14341 atattgattc attagttgat tcgatcatga atcaatatga agacgcgtta aaagatttgg
    14401 ttgatttatg aggtatcttt cgactaaaga aattatcaaa atcaacgcga aagttatttt
    14461 acgtcactcg ccaggcgaaa tgatcggtgt aaaagatgca aatgcattag atatggccgt
    14521 caaacagcct tcacaagcag tattcaatca agaactctat cctgaaatct atgaaaaagc
    14581 ggcgatttta gcaattaatt tagctaaaaa acatcctttt cataatggca ataaacgaac
    14641 tgctcttgtt gccatgttgt tgtttcttca attgaataat tgtccagttg ctttttctcg
    14701 tcaagaagct gttgatttta tcataatgat taccactagt tcattggatt tcgattcact
    14761 aaagtccaag ataaccagtt atttacgaca aaccgcaata aaatgaacga attaaaaact
    14821 ttatttctca attagatcag catgtcatta aggaatatgc tgat
//
LOCUS       DAFFSV010000066        14623 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFFSV010000066 DAFFSV010000000
VERSION     DAFFSV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.066"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            4..816
                     /locus_tag="LR110_001790"
     CDS             4..816
                     /locus_tag="LR110_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBL4822746.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="LR110_001791"
     CDS             813..1613
                     /locus_tag="LR110_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBL4822747.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="LR110_001792"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="LR110_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBL4822748.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="LR110_001793"
     CDS             3110..4819
                     /locus_tag="LR110_001793"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBL4822749.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="LR110_001794"
     CDS             5031..9389
                     /locus_tag="LR110_001794"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBL4822750.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="LR110_001795"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="LR110_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBL4822751.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="LR110_001796"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="LR110_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBL4822752.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="LR110_001797"
     CDS             11227..11520
                     /locus_tag="LR110_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBL4822753.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="LR110_001798"
     CDS             11517..11828
                     /locus_tag="LR110_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBL4822754.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="LR110_001799"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="LR110_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBL4822755.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="LR110_001800"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="LR110_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBL4822756.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4982 a         2598 c         3254 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctc
//
LOCUS       DAFFSV010000067        14563 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFFSV010000067 DAFFSV010000000
VERSION     DAFFSV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.067"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(172..1119)
                     /locus_tag="LR110_001801"
     CDS             complement(172..1119)
                     /locus_tag="LR110_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBL4822757.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1309..1578
                     /gene="rpsN"
                     /locus_tag="LR110_001802"
     CDS             1309..1578
                     /gene="rpsN"
                     /locus_tag="LR110_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL4822758.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1754..2116)
                     /locus_tag="LR110_001803"
     CDS             complement(1754..2116)
                     /locus_tag="LR110_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL4822759.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2127..3248)
                     /locus_tag="LR110_001804"
     CDS             complement(2127..3248)
                     /locus_tag="LR110_001804"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBL4822760.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3264..3614)
                     /locus_tag="LR110_001805"
     CDS             complement(3264..3614)
                     /locus_tag="LR110_001805"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBL4822761.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3744..5255)
                     /locus_tag="LR110_001806"
     CDS             complement(3744..5255)
                     /locus_tag="LR110_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL4822762.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5577..6929)
                     /locus_tag="LR110_001807"
     CDS             complement(5577..6929)
                     /locus_tag="LR110_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBL4822763.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(7082..8158)
                     /locus_tag="LR110_001808"
     CDS             complement(7082..8158)
                     /locus_tag="LR110_001808"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBL4822764.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8354..10099
                     /locus_tag="LR110_001809"
     CDS             8354..10099
                     /locus_tag="LR110_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBL4822765.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10379..10816)
                     /locus_tag="LR110_001810"
     CDS             complement(10379..10816)
                     /locus_tag="LR110_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBL4822766.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(10800..12173)
                     /locus_tag="LR110_001811"
     CDS             complement(10800..12173)
                     /locus_tag="LR110_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822767.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12495..12983)
                     /locus_tag="LR110_001812"
     CDS             complement(12495..12983)
                     /locus_tag="LR110_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4822768.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(13005..13607)
                     /locus_tag="LR110_001813"
     CDS             complement(13005..13607)
                     /locus_tag="LR110_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL4822769.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(13676..>14563)
                     /locus_tag="LR110_001814"
     CDS             complement(13676..>14563)
                     /locus_tag="LR110_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBL4822770.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT         4171 a         3101 c         2590 g         4701 t
ORIGIN      
        1 tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg aaagataatt
       61 ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca ttttttgtct
      121 ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt cttatttttc
      181 gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat gtgcttggat
      241 gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg ctcctttttg
      301 gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc ttggaacaaa
      361 cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt catttaatgc
      421 aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga tacttccttt
      481 cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat ttgttttatc
      541 ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt tactggatgc
      601 actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg acccgaccgc
      661 acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca aatggttgat
      721 tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc aactcatttt
      781 atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt aagtcccgcc
      841 gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg aaccaatctc
      901 tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg ttcgtatctc
      961 tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg cttttatttc
     1021 tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt caaaaacatc
     1081 ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct ccctttttct
     1141 ttaagtttac gtttatgcct ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg
     1201 attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta tctaaaagcg
     1261 taaagattac gatttacgct aataaaaaat aattggagga atttgttgat ggctaaaaaa
     1321 tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc tgctttacgc
     1381 caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt agatgctcgt
     1441 ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata catgcgtaaa
     1501 ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat tccaggagta
     1561 aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg cgcatggacc
     1621 actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc tggtactaga
     1681 agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag gctgcatggt
     1741 tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca ctaattttca
     1801 atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt tctttcaatc
     1861 ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt tctattcctg
     1921 ttccagtagc tttgatccca atgtgagtcg gaagcttagg tttagccatt tttgccgtta
     1981 tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga ataaccaaaa
     2041 ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca aaataaatat
     2101 ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat ccgctgtcct
     2161 aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc ctgcatagtg
     2221 atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg aaattcttga
     2281 ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca gtagttccct
     2341 tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata accgatcggt
     2401 atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc gattccttca
     2461 cctgctggga gttttttgac ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat
     2521 ggataaactt ccggtagtgc gtgcccagag ggattaagtc cgtacattgc tactccataa
     2581 cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt gctgacatgt
     2641 acatatcgag gaagttcggg aagttttttc agcacttcta taaagcgttc tttttgcaga
     2701 ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa accttcccat
     2761 tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc aggtgtacaa
     2821 aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg cgtctctagc
     2881 ttacttttct ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc tgcagttact
     2941 gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt caagataagg
     3001 atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt tgaaacacaa
     3061 aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc atgaccgtat
     3121 ccgttcgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc aacttcattt
     3181 gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg gtgccaagcg
     3241 acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa agctgtttca
     3301 tttgtatgag taatactgac ccatacattg ccttcatgag gtgaacgcgt cacaacaggc
     3361 tgaccatttt cattttttaa aatctcgaca tcttgaaagg taacttttcc gatcccagtt
     3421 ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg
     3481 cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat tcgtgcaatg
     3541 aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc atcgatccca
     3601 attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc attctaccaa
     3661 attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt ttctcttaaa
     3721 aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt
     3781 ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct ataaccgcct
     3841 tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt tccgccacca
     3901 cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc tggtgtgatc
     3961 ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa
     4021 tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt atctagacca
     4081 ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc tttgaatgct
     4141 tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca
     4201 tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc ttttccggca
     4261 cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat atcatagttg
     4321 tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt cgctacaagg
     4381 atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa
     4441 tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc caattcgtct
     4501 acacggcgtt ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac atcaaacaag
     4561 cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg atcgatcaag
     4621 tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat gaatttaacg
     4681 ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca
     4741 ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa catttcatct
     4801 gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg
     4861 tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa gccacggatc
     4921 tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt gtcgcgccct
     4981 aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc gataactaaa
     5041 ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc aaatgcagct
     5101 gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa tgctagtgga
     5161 atcgtttctg cttggatagg cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac
     5221 aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata tggttgttaa
     5281 ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt
     5341 tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc accgaaaaat
     5401 cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct ttatcattat
     5461 tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt tctcttcatc
     5521 tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac agatttttat
     5581 ttcatttgct gaagttgttc taccacttca aataagccca tgctgttgct gccttttagc
     5641 ataatactat ctgttgcttc aagattttgt ttgatagcat caatcaattt attcttttct
     5701 ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc ttttagtgca
     5761 tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat atgttcactc
     5821 atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc taacacagcg
     5881 attctgcgtc cttttgttgg cagattttta aaagtatcta ataccagtcc catggctgtt
     5941 ggatttgcat tgtagacatc gctcaaaata gctgcaccat ttcctgccgt tagccattcg
     6001 gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg tatggacagt
     6061 ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata acttccaggc
     6121 agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt tccttttgct
     6181 gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt ttgtactaat
     6241 gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa tccgtctgat
     6301 acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga cccaagattt
     6361 tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc cataagtgaa
     6421 agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt ttctgtatct
     6481 tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc
     6541 ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt ggtggtttta
     6601 ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta ataggttgcc
     6661 aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatt ttttggagct
     6721 tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc ttgctggata
     6781 aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc ggttatcttc
     6841 cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct gcccacggct
     6901 tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa
     6961 agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat ttctctattc
     7021 gtatttagaa gctagaatga acgcggttac gcaatcactc cagcctcttc tttatttctc
     7081 tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt cctaactgga
     7141 tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag agtgggtaca
     7201 tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat tcatttttat
     7261 ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg taagctaact
     7321 tcgcgtactc ttgcgctttt tgataaactt cttctggcac ttcggctgga atctgcattt
     7381 cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct tttacgactt
     7441 caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg atttcgcgcg
     7501 cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt gctaaagcat
     7561 tttgcagctc ttctcggttt tctgcttttg taatgccgac actagaaccc atattcgccg
     7621 gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact tttttaggat
     7681 tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca ccagcagctt
     7741 gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat acgcctgcgc
     7801 cgacataagg catattcaat gtctctaaga agccttggat cgttccatct tccccgtttg
     7861 gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc ggattgatca
     7921 cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat
     7981 ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct tcttttgtaa
     8041 taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact gaaaatgcgg
     8101 aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta atcttcaaaa
     8161 gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta gcacaaaaca
     8221 aaaataaacg aataacttat ttataaaaaa acgagccagc ttctaaaaga acgagcgtca
     8281 tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat gataaagaaa
     8341 aggagggatg atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt
     8401 gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa
     8461 aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc tttctgttcg
     8521 agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag atagtgtacc
     8581 tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc tttcattatc
     8641 agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg ggcaggctca
     8701 tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag atccaacacc ctaacatcgc
     8761 cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga tcaaatacat
     8821 tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac gttccttgat
     8881 caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc aaaagaacaa
     8941 tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga aaaaagtgat
     9001 cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta atgaacgccg aaaacaaaac
     9061 tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg caaagctttt
     9121 agttgagaac tttatgaaac aggtgtttga tgatggtttt ttccatgctg atcctcatcc
     9181 agggaatctg ctattccatg tactaacaaa agaagaacag acacaagcta gccgaaaaac
     9241 tgaaacagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca cttctgcaga
     9301 agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg ggcatctttc
     9361 tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga aagatgctta
     9421 tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg
     9481 gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta ttgatgaaat
     9541 caacttgcaa gaagtattca cacatgtcgt gacgatttgt catcaaaata atctgcagtt
     9601 cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg tgatccgcgt
     9661 cctcgatcct gaagtttccc tcatggaagt cgcttcgcct tttgctcaac attattttct
     9721 cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat tggaagggat
     9781 gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt ggacttcggg
     9841 acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag
     9901 tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag ggtcctccct
     9961 gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag ggatatttac
    10021 gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga tccaaatgta
    10081 taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca agaaataaga
    10141 aggggtttta cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga
    10201 ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata
    10261 agaacaatca aaaagggaac atggaacaca tttatcctgt ttccctgttc cctttttgat
    10321 gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt ggtaaacctt
    10381 agtcaattgc cttttttaac gcttgacgaa aagacatatg atctttctcc gtaaagggtt
    10441 ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa cgttgttcta
    10501 gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac actcgcacac
    10561 ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag tcattcttct
    10621 taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg tagacgattt
    10681 ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg ataaccacgt
    10741 caagaccata ggattctcct aattttatga catcttcttt tactggagaa ccatctccat
    10801 caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt tactaatccg
    10861 ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac actttctatc
    10921 ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc catttctttt
    10981 gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc tttattttga
    11041 gtagtatact tgacaacgat ccggcgatcc attggaatag ctgtatcagt ttgataatat
    11101 tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa gacacctatc
    11161 gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag caattgcata
    11221 gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt tgctgcaatc
    11281 aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc gtacagtatg
    11341 ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt tagaaaagta
    11401 aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa aacaaacacg
    11461 aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct ttcatcgaat
    11521 aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa taaaacaagc
    11581 tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta acgcggataa
    11641 tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca
    11701 atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca gatagctgca
    11761 aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg aaaattgacc
    11821 gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact tgaaaaataa
    11881 aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc accaatcagc
    11941 agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt gactaaaaat
    12001 agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag ccaattatgc
    12061 gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt acttaagata
    12121 aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa catgacaatg
    12181 gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa ggggaaataa
    12241 accaatttta cagaatatat tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt
    12301 ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca aaagtcttgc
    12361 ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg tgcctccaaa
    12421 atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa tttactttaa
    12481 cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat ttgaaatcct
    12541 tgaccgatat ctgaaataag ttcttctgta atctcaaatc cttcatgctt ataaacctcg
    12601 attgcttgat gattcccctg attcactcgc aaaaagatct ctctttcctt tgtttgcgca
    12661 gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg tactttattt
    12721 agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc agtgtaaccg
    12781 acagtctttt cttcgctttt taataaaaag taggacacac cttctgtcag ttctttttga
    12841 atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg accaatgatc
    12901 ggcacaaaaa cttccggcca tacttcttgt atgactgcca tcagctcatc cagttcattt
    12961 tctgttactt ttattttttc catacaccca cgtccttttt tacattataa atgaccttgc
    13021 gcttgatgga ataaaaagag catgatcatg ctatacacac ccaccgacca cggtttcaag
    13081 aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc tactagacag
    13141 atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt tttaatatct
    13201 ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga taaaataagc
    13261 tggacaaagg ctacgccata gtaacggaca agcaaaaatc cgattgcttc cccaatcact
    13321 aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat gcccgctact
    13381 gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcaa cagaacaaat
    13441 atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt gctcgatcga
    13501 aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaaaaacag accaataatg
    13561 ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta tattccccac
    13621 atcttttttc atttattaaa actgcctcct tatgaactga accccaaaag ttgaactatt
    13681 taatggactg tttccgatat tctactggag atagtccatt taactttagt tttatccttt
    13741 tgttattata ccacctaata tactcattta atttactttg aaatatctca attgatcgga
    13801 atttttctcg ataaaaaaac tctgatttaa gcacaccaaa aaaattttct attacagaat
    13861 tatctaagca atttcctttt ctagacatgc tttgaataat gttattctct tttaattttt
    13921 tttgatattg tggcatctga tactgccatc cttgatctga atgtagaatc agtgaaccct
    13981 cagttccctt ttttttaatt gcttgttgaa gcatttcttc aatcagttta tatgttggac
    14041 tagttgatat actataacta attatttctc cgttaaataa atcaagtata ggagatagat
    14101 agatttttct tccttttatc ttgaattcag tgacgtctgt cacccatttt ttattgggtg
    14161 tatctactga aaaatttcgt tttaacacat tcttggcaat ttttcctact gttcctttat
    14221 aagatttata tcgctttatt cggatttgac aggtaagtcc catttgggac attaattttc
    14281 taactgtttt atgattgatt gtatatcctt tcatctttaa cgctaaagtg actctacgat
    14341 aaccataaga gtttcttgat tcttttacaa tcgctgtaat ttcttgcttt atcttgctat
    14401 atttatctgg cttatctaat ttttttaccc agtaataata agttgacttc gctaattgtg
    14461 caattgaaag taatagattc aatttaaatt cttttttgag ctgaaggatt gttttaacct
    14521 tgatttcttc ttgctcaaat cttgttcttg aatcaaggtt tct
//
LOCUS       DAFFSV010000068        14306 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFFSV010000068 DAFFSV010000000
VERSION     DAFFSV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.068"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            35..1375
                     /locus_tag="LR110_001815"
     CDS             35..1375
                     /locus_tag="LR110_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4822771.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1593..2036
                     /locus_tag="LR110_001816"
     CDS             1593..2036
                     /locus_tag="LR110_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBL4822772.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2540..3958)
                     /gene="nhaC"
                     /locus_tag="LR110_001817"
     CDS             complement(2540..3958)
                     /gene="nhaC"
                     /locus_tag="LR110_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL4822773.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4361..5584)
                     /locus_tag="LR110_001818"
     CDS             complement(4361..5584)
                     /locus_tag="LR110_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBL4822774.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5615..6454)
                     /locus_tag="LR110_001819"
     CDS             complement(5615..6454)
                     /locus_tag="LR110_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL4822775.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6471..7151)
                     /locus_tag="LR110_001820"
     CDS             complement(6471..7151)
                     /locus_tag="LR110_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822776.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7153..8226)
                     /locus_tag="LR110_001821"
     CDS             complement(7153..8226)
                     /locus_tag="LR110_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4822777.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8282..8520)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8563..8796)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8873..9544)
                     /locus_tag="LR110_001822"
     CDS             complement(8873..9544)
                     /locus_tag="LR110_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL4822778.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9631..12309)
                     /gene="mgtA"
                     /locus_tag="LR110_001823"
     CDS             complement(9631..12309)
                     /gene="mgtA"
                     /locus_tag="LR110_001823"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBL4822779.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12968..13405
                     /gene="ndk"
                     /locus_tag="LR110_001824"
     CDS             12968..13405
                     /gene="ndk"
                     /locus_tag="LR110_001824"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBL4822780.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13427..14293)
                     /locus_tag="LR110_001825"
     CDS             complement(13427..14293)
                     /locus_tag="LR110_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL4822781.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
BASE COUNT         4091 a         2996 c         2539 g         4680 t
ORIGIN      
        1 ttttgattgt gaatttaaaa gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca
       61 acttagctcc agacatatca ccatgttagc tttaggtgga gcaatcggtg caggtctatt
      121 taaaggaagt ggcgaagcga ttgggatcgc cggcccttct gtattgattg cttttttgat
      181 tggtgggctg attttatata tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg
      241 cgacacgcat cacggattat ctggtttgat acgcccttat ctaggtgctc attctgctga
      301 ttttactgat tgggtctact ggtctatgtg gattatcaat atcatcgctg aagctgttgc
      361 agctgcttcc tttttgcagt tgtggttccc gcacattcca gcctgggtat ttgtttttat
      421 gttggctgtt ttaactacga tcatcaatct ttattcggta cggttattcg cagaaacaga
      481 atattggctg gcttttgcta aaatcagtgt cattatctta ctgatcattt ttgctactta
      541 tcttgtcggt cagcaaatgt tgggcacagg cgttttccct acccttcagc aattgactga
      601 ccatggtggg tttactcctc atggtatgaa gggaatcatc agttctttgc ttgtagttat
      661 ctattcttac ggcggttctg aactgattgc tatcacggta agcgaagcag atgatcctaa
      721 acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga atcatttcct tttacatcat
      781 tcctttgttt ttgcttttga ttctttttcc ttggaatact ttagcaggca cgaatgtcag
      841 tccttttgtg atggtatttg aaaaaatgaa tatcccattt gcagcggata tcgtcaattt
      901 tgtgattgtc cttgctttgt tctcatctat caattcgggt gtgtatgcat cttcaagaat
      961 tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga aaactagctg ttttaaacaa
     1021 gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt gttctttatc ttggtgtagc
     1081 tctttcttat tttgtcggtg acaaattatt tggctatctg gcaggttccc tttcgtatac
     1141 agtattattg atctggatca ttatcagtgc ggcatccttt gtactggcac taagaaaagg
     1201 aacattatgg gataaaggaa tcagcttttt tgcattagct gtcttaggat tgattttcct
     1261 tggtatcctg ttcacgaact ccattggggt aactgtattg actggcctgc tttacctgtt
     1321 catttatttc agttatcaaa aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg
     1381 atagaaaatt ttattttatt tatgtggaat agatggagct attgaagaaa ggagttgtga
     1441 caaaagtctt gttgcaactc ctttttccga ataaacagtc tcatgacctc ttggtaaacg
     1501 gtgttcaaaa accctctgtg caaaataatt gattgttctc catgctcatg acatgataga
     1561 agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa gtattggaag tttttgttga
     1621 tgatcggtgt ttcaacggta gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg
     1681 atcatatctt cttcagtcgt acacgagtat tcatggcgtt gatgatgggt gcagtcatgg
     1741 cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa caaaaagata aatgttatga
     1801 tcttagccgc tagtcttctt gtttttgccg gttccttgtt tatggtacga agccaaacta
     1861 ctgtcaacga cacagcttgg atgaaggcca tgattcctca tcattctatc gctatcctgg
     1921 caagtaaacg agctgattta aaagatcctg aagtaaaaaa attagcggaa gaaatcattc
     1981 aggcacaaga agaagagatc gacaaaatga aaaaaatgat caaagaaatg gaatgaccat
     2041 tatccgtcca aataaaaaaa gaggatgttc cagaagttat cagcttcaag aaataagaag
     2101 aagctacttc tggaacgccg tgaataaggg gttgtgaaaa agctgccctg catcaagtaa
     2161 taagaacagc caaacaaaaa tagcttctcc tatttttcgt ttggttaggc ttattaccgc
     2221 agatgcaagc ttttgaacac cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag
     2281 tattagagag acaaatggaa aaatagttcc tcatattttg ccattttttg agctaatacc
     2341 gagggtcagc tgattgaaca ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc
     2401 gtattaagaa aaattttgtg aaatggcttt ccacattttt gtataaattt ggcttaatac
     2461 cgagaaactg gcttttgaat accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc
     2521 acaatccctt tttatgtttt tattgttcta attgcgctgt cttgcttact gcttcttgcg
     2581 gtttcacaaa tttttttgag attccccaac ctgtgaaggc actgaccatc gaaaacacag
     2641 gagaaagcaa actgaaaaat acgaatggca agtaagtgag tgtaggaata ccaaatgtac
     2701 ttgctacgaa acttccggca attccccaag gcactaagta gttgattact gttccaccat
     2761 cttctagcgt tctacctaat acagttggat caaatcctgc tttttcataa gtttctttaa
     2821 atgcattacc aggcagaata acagatagga attgttcacc aataaaaata tttactccga
     2881 tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt cgtcatcttt cttgatactc
     2941 gttccatcac ggttgagatc aagccaaatt cgatcaacaa tccacctaat gacaaagtta
     3001 gaataatcaa tgacacagta ggcatcatac tttcgattcc tccgcggcta agcagtgaat
     3061 cgatttgttg gtttccagta tttgaaacaa accctgattc gatgacccca gccaacttcg
     3121 ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac tgctacagcg atattcaaca
     3181 aaatcgttaa aattgccggc acctttttcc atgcacacag taacattaaa aataaaggaa
     3241 tcagtgacca gattgaaata gtaaagtttg cttctagaac atcagtcact tcacgaatag
     3301 atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa
     3361 caaatgccgg gacagttgac cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa
     3421 caatagctgc tgctagattt gttgattcgg ataatggaga catcttatct ccaaaaacgg
     3481 cacctgaaac aatagctcca acaaccagcg ctggatttaa gcctagtgtt gtcccaatac
     3541 cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc actccctaca gcgctaccta
     3601 cgattgcaca cacaacaaat acagaaggga caaaccactg gacactgatc aatttgaacc
     3661 caatgaccat tagagttgga atgatccctg cttgtatcca tacagcaatc aatgaaccga
     3721 ctaaaataaa aataaagatt gggatgattc ctggtttgat gccattgatg attccttcgt
     3781 gcagccaatc tgtcgggtat ctttttatca ctgcatacaa catgaccatt ccgatcgctg
     3841 ctaagatcgt gatattaggt gagaccttta atccgacaac gccaatcgca atgcctaaaa
     3901 caataaccat taatacaagt gttgcttcta aaaaacttgg tgttcctttt tttcccatga
     3961 taatctctcc ttttttagtt gttcttatgt tctaaaaaag ttaagatatc ggattactcc
     4021 gccgcaattc atccgcgatt cctcctttat aaaaataaaa agacctgttt taaactaagg
     4081 acgaatgact ccgcggtacc accttaatta aaaacagatc tgtttttcac tttcaaactt
     4141 ttattcaaat gtatggactt tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg
     4201 accaccgact ttctgaacaa aacacctaac cgctactgcg tgaaaagttg acttgtacga
     4261 agtttacctt ccataagatc atttgtcaat gaaaaagaaa atattttttc atttttctga
     4321 aaattatttt tataactttc acacacaagt ttatttttta ctagaaagta gcgccctctt
     4381 gtttttttct tgctactatt tcttgatgtt cttgcttgat tctttctaat agctttggct
     4441 ctttcaacag atcaagagct gtattggcga gtagttctgc tgcaatcgca atagaagcaa
     4501 ggcctttgtc acttttagca gcagctttga attcttctgt gtgtccggca atatatgtct
     4561 cagagattgc taccgtaggc tggattgttg gtatgacttg gctgacattt cctacatcag
     4621 aagaaccgag acttgttctt tgttccgttt ctatttcttc atctggaaca cctgcttctt
     4681 ttgcatgctg gaagaataag tcatcaaaag aaggagtaac gatgacatca tcaactgcat
     4741 tttggaacaa tccaaactca aaagtcgtgc ccgtacttat cgcagcacct cgtacgatat
     4801 tttccacttt ttcatatact ttatctaatg tttgccgatt tcctgcacgt aaatagaaac
     4861 gtccagcagc gtaatctgga atgacgttgg cagctactcc tccgtctgta atgatcccat
     4921 ggatattgac atcttttggc aattgcaagc gtagagcgtt gattccatta aacacttgga
     4981 tcaatgcttc caaagcattt actccttttt caggtgctgc tgctgcatgg gaagcgacac
     5041 cgtaaaattt gatgtcgact ggatcattcg ctaaagattc tgtcgttttc ccataacgaa
     5101 aagctggatg gacgcataac gctacatcga catcctcaaa aaaaccttct cgaacaaagc
     5161 tgcctttagc tgatccgttc tcgccccctt cttcccctgg ggtgccatat acacggattt
     5221 ctccaccagt ttcatcaatg aattgcttca ctatgctggc ggctaatacg gagtaagtac
     5281 caaacaaatt atgcccgcat gcatgaccaa tacctggtaa agcatcgaat tctgccaaaa
     5341 aggcaattgt gggaccgggt ttattggaaa tataacgtgc atcaaaggct gttcggtgtc
     5401 cagctacttc tttttctacc caaaaccttt ctttttctaa ttgtttaatc aacgctgctg
     5461 aggaataaaa ctcatagtta cttacttcag gatgttcgtg gatatctaga gcaagtgctt
     5521 gatattctgg taaacgatat tggattagat ccttcaattg gtttgttgag attgttgttg
     5581 tcataaaaaa ttcctcctat gctagtcatt tttcttattt tctcccgaat gtttcccatg
     5641 caggcacgct tgatcctttt gatgttttct cgattacttt gactgtgtct tctgtttgat
     5701 aagcgtctac tattttttga tacgttttat tgttttcatc ttcttttctt gcaacgatga
     5761 tattcacata aggacgtgca tggtcatcca ctggttctaa gaagatggca tcagaagcag
     5821 gattaaatcc agcatctaca gctacaccgc tattgatcac tgaaatgtct acatctgaca
     5881 aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc tagatttttt ctattctcgg
     5941 taatatcaga aacagtgggc gcttgtcctt ttgccttatc cactttgatc aatccagcag
     6001 tttgcaataa cagaagagct ctgccgccat ttgtaacatc attagggatt gctacttttg
     6061 ctccgtcttt gatatcagct acatccttca aagtgttaga ataaattccc agcggtgcat
     6121 tgacagtatt tccgatcgat actaaatcag ttccgtgttc tttattgtaa ttgtctaaaa
     6181 agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc tgctaaagcc acatttggtt
     6241 gattgtaatc tgtaaatttc acgtattcca aattaatgtt ctcctttttc aaacggtctt
     6301 tcacgttgtc ccatacatct gtatcttctc caataatccc tagttttacg atagtttctt
     6361 ttttgtcagt tgtatctgct tttccttggc tacaccctgc caaccaaata attgctacaa
     6421 atgtaatgac tacaaaacga ataatttttt tcattttttc tcctccactt tcaatgtgta
     6481 atttttttaa tcagttgatt actgataaat tgacttaaaa aaactaagat tagaatcaat
     6541 aatgtagcca caatcgttac atctgtttgg aatcgattat accctctcga tatagcaaga
     6601 ttccctaatc cgcctgcacc tactgctcca gccatggtag tcagtccgat cacgttgatg
     6661 atagtcaacg ccgagacccg aatgatacca ggcaaacctt ctaacaaata cacacgtaaa
     6721 atgataccga ttggactggt tcccatcgct tctgccgctt cgatcacccc aggatcaact
     6781 tctagcagtg cgttctcgat ttgacgagca aaaaacggaa caactccaaa aatcaatggc
     6841 actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga aacgagtagt caatgctaaa
     6901 agagaaagca agataatgaa agggatggaa cgaatgatat tgatcagctt atcaagtatt
     6961 tcaaatagca gacggttagc tagtaatcca ttagggcgtg tcactactaa cgtcacacct
     7021 aagatgattc caatcatccc gccaatgaat gctgtccaaa aagacatata tagtgtttgc
     7081 ttcgtcgctt cgacaaattc atccggaatc aaataaacat tgggcaaata tgttttaaag
     7141 aaatcagtca ttttatcttc ctccttcgat cactttgata cgactatcta aatcgttttt
     7201 ttgtaaaata tttattttta caccagattt gatcaaataa tccaatgctt ttttccgctg
     7261 tttggtttct ccagctaaag taacgatcaa actccctaac ggcgtttctt gtaataactc
     7321 tacattgcca tataaaatat tcgctgttac ttgatatttg ctgtacagat gagcgattag
     7381 aggttcgttc gtctgatttc caatataact taattccacc agccatttat tggcgatctg
     7441 atcggcaaaa gcagaatggg caatgatcgt ttctaatgct tggtcaagat gagtagctgt
     7501 tcgaataaaa tctttggtca attcttcttc aggattgctg aagatctgga tgctgctacc
     7561 tttttcaacg attcttccat cttccataac cgctaccttg ttgcagattt ctttgacaac
     7621 ttgcatctca tgagtgatca ggacgattgt taaacctaac tggcgattca atttttttaa
     7681 caatgcaagg atctgaaggg tcgttttcgg atcaagtgca ctggttgctt catcacataa
     7741 taatacctta ggttcattcg ccaaagctcg tgcgattgct actcgttgtt tttgtccccc
     7801 cgacagttgt ttaggataag cagatgcttt ttcttccaaa ccgacaagtt ccagtagttc
     7861 attcactttt tgacgacgtt cctgtttcga ttttccagaa tactttaaag aaaaatcaac
     7921 gttgtcaaaa atagttcggc tttccattag attgaaatgc tgaaagatca taccgatggc
     7981 ctttctttct tttcgcaatt ctttgggaga gagctgactc agattcgtct gattgacaat
     8041 gacttctcct gctgttggtt tctgcagcaa attcatcaca cgaactaacg tacttttccc
     8101 tgcacctgag taaccgacga ttccataaac atcgcctttt tcaatcgtta aatctacaga
     8161 atctactgcc tgtatctctt tttctttttg tttgaaacat acagaaacat ctcgcaattc
     8221 aatcattaaa attcctcctt atctaaaaaa agcgtcctca agaaaatgaa aatgtcattt
     8281 tcttgaggac gcttttttgc gtgttaccac ctcatgttca agctgtgttc acacacaact
     8341 cctcttcaag tacgatcata ctctagcaag atatcggtcg cagccgtcat cgccttacat
     8401 ctatgctcgg gatgaattgc tcgatggcca ttttccaata gtcgttcctt gcttcttctc
     8461 agctaccgaa gctctctgtc aaaggttctt ctatcgtact taccatttca atgcaaataa
     8521 tattcaaaaa aaaagtcctt ttactaacat aaactatgtc agtaaaagga cgacgtatcg
     8581 cggtaccacc ttttttcgga agtttccttc cctcaagccc aacatctcaa aaagagagat
     8641 tggcgttcgt tttaacggtc gaacagttcc gaatttgcct acttatcgac aaattattct
     8701 ccaaggccat tttcagcttt tcttacttga ctcttctcac cagccagagc ctctctgcaa
     8761 agtttccaaa cttactttcc ttttcaacga aatcgatatt ttgttgttgg gattaatcta
     8821 catgaggaaa actgaaaagt caagtacgaa attcaaatta ttttttctat atttattttt
     8881 ctgttttttt atttaacatt tcaataacag ttcgaagatc ttttccttcc gcaagaagtt
     8941 tcaatttttc cttttcttcg acggtataat ttcggacaaa actggaatca ggtatcaaaa
     9001 caacgcctaa tcctgctcgt gaagcagctg ttgctcctac taaagaatct tccactgcaa
     9061 aggctgaact agcttctaat cctgttcgtt ccaaagctgt cagataagga atcggtgaag
     9121 gcttgttctc cgacacttcg tctccgaatg taaacgtgtc caagtaatca gctaatttaa
     9181 agtgatccaa gatatctgtt gctcttttcg taacagtggt cgtagcaatg cctagtttca
     9241 tatgacgttc ttttaagtct gccaatattt cttttgcata tggttttaag tggatggctc
     9301 cgcttgtcag ctgttgataa aaatattctt cacgcttcgc tcggacttcc tgtacttttt
     9361 cagggctacc tgctgctttc actaaaatat caaaactttg tttccagttt tttccgcccc
     9421 aagcgtcaac gatttctttt gggatatgga tatcaaattg ctgaaatccc caaagccagc
     9481 catcacgata cgttttttct gtgtcaacta acaaaccatc taaatcaaat attgcacctt
     9541 tcatctatac attaatcctt tccaaaacaa tacacatagt ttagcacgac tggtccaaaa
     9601 agaacagaag caaagaacca gccttctcgt ttataagagt tcttggaatc gacggatata
     9661 gattttcttc acagctgtca caagcgccag ataagcgatg actgtagcaa ataaccataa
     9721 ccagtaattg ccaggaagtg gcatcaagcc taattcagca ccaaaacttg taaaaggaag
     9781 gacagaaccg acaataattc ctaatgtagt gataacaaac atggcaaatg ttgcgttgct
     9841 ttggataaat ggtactttcg gtgtacgcag tgcatggagc actaaggttt gtgtccatag
     9901 tgattcgaca aaccatcctg catggaataa tgcgatgaaa atcactcgct gtgaactatc
     9961 caatgtatgg aaacttccgc ccactacagc aggacaaata acaaaataca tcaacaagta
    10021 agtagtaata tcaaatatcg aacttgttgg accaaagtaa accatgaatt ccccaatact
    10081 agatgcattc catttttttg gctcgtccaa atattcttta tccatccgat cccatggcaa
    10141 ggaaatgcag gaaacatcat agatcagatt caaaaacaaa agttgtaaag gcagcattgg
    10201 taaaaatggc aggaacgtac ttgcaatcag cacggaaaac atattgccaa aattagaact
    10261 tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt tctcgtccag aaagaaggcc
    10321 tctttccaaa atcgtcaaat ctttttctaa aaggatcacg tctgcagatt ctttcgcaat
    10381 atccacagct gtatctacag aaataccgat atcagccact ttcattgcag gagcgtcatt
    10441 gataccatct cctaaaaatc cgacagtatg accattttta cgtaaagctg tcgtcaaacg
    10501 tgctttttgc tgaggattca attttacgaa tacttcatgg tcttcagcta cttgtgctaa
    10561 ttcatgatca ctcatttccg agattttctc accggtaacc aattcattga tctctagacc
    10621 tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc ccagtcagaa cttttacagc
    10681 tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt gtttcttttg gtggatcaag
    10741 gaaagcaaga taaccaatca aaaccatgtc tgactcatct ttgactgaaa attctccaac
    10801 gacgcttgga ttggtctttt gtgcgacacc aatcactcgt aagccatctt cattaagtga
    10861 ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt aatggtgtaa cagtaccgcc
    10921 aagatcaata aatttagaaa tgttcagcat ttcttcaatc gcgccttttg tgatcatttg
    10981 tgttttacca gctggatcag caaccacaac actcatacga cgacgttcaa aatcaaaagg
    11041 aatttcatcc actttttgat aattgatctt atctgtatct aaagtttctg ttgcttcatc
    11101 aatgatagcc acatctaaca aatttttcag ccctgtttga tagtagctat ttagatacgc
    11161 atggcgcaaa acacgatcat cttcatttcc ttcacaatcc atatgatatt caagaatgat
    11221 tttatcttgt gtcaatgtcc ccgttttatc cgtacataac acatcgattg caccaaagtt
    11281 ctggatggca ttcaaatttt tgatcactgt tccttttttg gccatcacac ttgctccttt
    11341 gaccaaattc gtcgtaacga tcattggaag catttcaggt gttagaccaa ctgctacaga
    11401 tagtccaaaa agaaaagctt ctagccaatc cccttttgta agcccattga tgacgataac
    11461 tgctggagcc atcaatgaca taaatttgat cagtagcatg gatgttttat ggatgcctaa
    11521 ttcaaagctt gattgaattg gtttttcaga tagcgtttct gctacatgac caaatagtgt
    11581 ttcatttcca acagaaacaa tgattccttc cgcacttcca ctgatgacat tacttcccat
    11641 aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg tcttcacata ctttcgcatg
    11701 cttttctact ggatagcttt cccctgtcaa tgctgcttgg gaaatgaata aatcctttgc
    11761 ggacaataaa cgcatatccg ctggtatcat atcccctgct gaaagccgaa caagatctcc
    11821 acagacgatt tcttccatag gaatctctgt gtactttcca ttacgtttta cagctgccgt
    11881 tactttcaca agtgatttca gtttttcagc cgcttgattg gaacgtacgg actgaatcaa
    11941 cgtcattgtc ccgctgacga taaccatagt gaagataatg gctacaccga aaagatcttt
    12001 atctccagga gctgctaata catattccgt gatgaatgaa ataattccta atgcgatcaa
    12061 cactaatgta aatggcgtga tgtatgcttt caatatttct acaatgaatg gtgttttttt
    12121 gccatatgaa atcgcatttt caccgtaatt ttctcttgaa cgttcgattt gttcttctgt
    12181 caatccattt gctgatgcat ggaatagttc aaataattct tgtttcgttt ttccagcaaa
    12241 agctgcatag attttttgtg tatctttctt tttttccatc tcaatctctc ctttgtgtcc
    12301 aaaagacagc tgttctcgct gcttttggtc tttagctatt catcggatcg atagagaaaa
    12361 aactgaaagg ggctgagaac agatagataa tagccctggc ctttcctcta gatccttgtt
    12421 tttcgaatga ctgtcatcca tttgttctca tctcctttac taagtaattt taattcatga
    12481 cttccaatca tttttcgaat agttattttt ccaattagac cacctccttt ttatttttca
    12541 ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa tactcagcga aaggtttgaa
    12601 aaagcatatt aaaaaaagct tctcagatct catcgttgag ttttgacact gtacaacgta
    12661 aaaagcagat acctgctttt agctacctta tgaaaagcct tatctcgaca attcctgttc
    12721 tacccattgg cgtctctcga cgtttttggg cagtagccta tgtttgtata ggagcctcac
    12781 ctaacaaatc ttattcggtt ttccttcgtt aatataacaa ctaaaaaaga ggatgtcaaa
    12841 tcaattttcc atgtttttca tacttttttt aaaatacaca cgttatattc ctgtctcacg
    12901 cggttttatt tgtctcattt tccacctccc gctataatag aaatatagta ggaaacgagg
    12961 tgctgttatg gaaagaacac ttgttattat caagccagac ggcgtgagac gccatttagt
    13021 cggaagcatc atccaacgtt ttgaagcaaa gggactggcc attgctgaaa tgaagtttga
    13081 aactatgact cctgaattag caaaagaaca ttatcagcac ttgactgagc gcagtttttt
    13141 tgatgaactg atcgattaca tgacttccgg tcctgtcgtt tatcttgtgc tggtcggcga
    13201 ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca aaagctgccg atgctgttcc
    13261 aggtacgatc cgaggagact acgcgctgcc ggggactgaa aacatcattc acgcttctga
    13321 ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc ccgccattcg atataaacaa
    13381 accgtctgaa aaagcggcga agtgatacaa attctaaact gctggtctag ttgctgggtt
    13441 cagcaatggc cagcagtttt ttctctaagg acctccgttg attctttcct aacatatatt
    13501 cgagcactgt gaagaattct tggtagtctt tcgtcttttc ttcccctaaa gcagcagaaa
    13561 tcaatctttg ataataatct gccatcaatg tttcatagat cactgtactt ctttctttat
    13621 gtgataagct ccgtttcata tcttcattca cactacgtgc ctttttcaac cgaccatttt
    13681 ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag atactgctca tgattgttca
    13741 actgtgctaa tcgtcgcatt ctttttttga ttttcaaata attgttctca atgaaccgat
    13801 cactgaatat ccaaggaaaa tcagctagaa tagatagttc aaattgacca tagtctggta
    13861 tctgcatcaa gtattcttca atgattcggc attcataacg taaaaattca aattctgtct
    13921 cagatacttc tgttttttcg accatgtgaa gattcaaaac caaataatac agatcttctg
    13981 ttttcttaaa atgcctacgg ttattgctga tctctttata aggatcaaag ctgtcttctt
    14041 gtacccgatc tagcattcgt ctttttttct ttaactttat tggttcatcc agctggtttc
    14101 gcatccattc atattctgca agtgtcatgt tcattcgctc taaaaatgat ctcaatgtta
    14161 caaatgacat ttttttcact gaatttgtct caatcccgac tagagaagtc ttaaaacaga
    14221 ttccttccgc taattgggac tgtgtcaact tcttgttgac tcgaagtttc ttgatcaatt
    14281 caccatcata cataatagtt ctcctt
//
LOCUS       DAFFSV010000069        14269 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFFSV010000069 DAFFSV010000000
VERSION     DAFFSV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.069"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(324..893)
                     /locus_tag="LR110_001826"
     CDS             complement(324..893)
                     /locus_tag="LR110_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL4822782.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="LR110_001827"
     CDS             complement(967..2256)
                     /locus_tag="LR110_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822783.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="LR110_001828"
     CDS             2610..3458
                     /locus_tag="LR110_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBL4822784.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="LR110_001829"
     CDS             3472..4545
                     /locus_tag="LR110_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBL4822785.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="LR110_001830"
     CDS             4535..6706
                     /locus_tag="LR110_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBL4822786.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="LR110_001831"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="LR110_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBL4822787.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="LR110_001832"
     CDS             complement(9009..11195)
                     /locus_tag="LR110_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBL4822788.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="LR110_001833"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="LR110_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBL4822789.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="LR110_001834"
     CDS             complement(11417..11857)
                     /locus_tag="LR110_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBL4822790.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12404)
                     /locus_tag="LR110_001835"
     CDS             complement(11931..12404)
                     /locus_tag="LR110_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HBL4822791.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12714..14180)
                     /locus_tag="LR110_001836"
     CDS             complement(12714..14180)
                     /locus_tag="LR110_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4822792.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4080 a         2989 c         2530 g         4670 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaataaa
    12541 tggtgttcaa tcagctgacc ttcagcatta gctcaaaaaa cggcaaaata tgaggagcta
    12601 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    12661 cggtgttcca gaagtagctt ctgtcacaac ctcttgtaac aagtatttct attttaaggc
    12721 tgaaccactg tttctggttc agtaatttct ttgtttttgt ctcgacgagc aattgctggc
    12781 aagatcatac ctaataagat caagacaatt ggtgtgatga tattcgtgaa taattggaac
    12841 caccatgctt taggatcagc agtataatct acttttggca ccattcctag gatacatgca
    12901 aacgcagtga aggcaaagca ccaagcacca aagataaaac cgatttttgg atttttaaca
    12961 aatttatatt cagaattgaa tttcttataa gctttattca acatcatata tgccaagaac
    13021 acccataggt aacgcattgg catgacaact gagttcaagt tcgtcaacca ttttactaat
    13081 tcattcatat ctttgatacc aaagatcggc aacaaaataa tgattcctac tagtatacct
    13141 gtcagaagat aaccattcac caatgtacct ttacgattac gtttacgtag ccagtttggt
    13201 acaaattctg gatccgcatc tgctaacaag atttttagtg gtgcatcaat tgaaaaggct
    13261 aatgcggaga tttgtccaat catgtttgtc aatgcgtaaa tgatcatcaa tgagttaccg
    13321 ataccatagt aattacctaa aatttgaaaa gcactgtatg ccccatttgc catcaagtct
    13381 ttagggatat tatcgctagc aaacagcata cccattgcga ctgagcctaa tacagcacaa
    13441 atccctacca ttgctgctaa gaagatcatc cctttaggaa attcctttgc aggatttctt
    13501 gtagagttga cataagggga tattttttcc gcccctccaa ctgcaaatac tagcatcgaa
    13561 acggttgtga aataactgaa atcaaatgtt ggaatatatg ttttcacttg attcatatgt
    13621 ggtgtggcaa attccatcgt tggattgatg aacggtgccg ttacagctaa aagaataaat
    13681 aagatcgaca tgacgaacat cgctgttccg gccagtccgc caatcacttt caaagtagaa
    13741 agtccttttg ttgataaata taaaaaggca agaaatataa taagtgaaat aactgctacc
    13801 atttgtactg acatcgtatt tacgatattt ccatttcctt ggactgccca accaccagca
    13861 atcagaattg cttgaggctt ttgtgccaaa taaggaatat ggacgaccca gtaagtccaa
    13921 gcggcatagt atgccagtcg tttattagat gtcgtatttt cgacccatga actgacgcct
    13981 cctttactat ccttgaaggc tgatcctaat tgtccaacga tcaatgcgta tggaataaaa
    14041 taaatggcca aaatcaatag ccaagaggtg acaactgata ctccttgctg agcatagtta
    14101 ttaactacat tccccatgcc ccaaaccatg ttaaatgcaa ttaacgcaac agtaaaccag
    14161 cgtaattgtt tgttgttcat tcgttctctc ctttatgata aaaatttgtt atccagatgt
    14221 aatcatattc aatttttaac aaacttacaa gacagtgtta ttttatgtg
//
LOCUS       DAFFSV010000070        14038 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFFSV010000070 DAFFSV010000000
VERSION     DAFFSV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.070"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(24..254)
                     /locus_tag="LR110_001837"
     CDS             complement(24..254)
                     /locus_tag="LR110_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL4822793.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            541..879
                     /locus_tag="LR110_001838"
     CDS             541..879
                     /locus_tag="LR110_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822794.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1084..1323)
                     /locus_tag="LR110_001839"
     CDS             complement(1084..1323)
                     /locus_tag="LR110_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL4822795.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1299..1646)
                     /locus_tag="LR110_001840"
     CDS             complement(1299..1646)
                     /locus_tag="LR110_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL4822796.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2378..2959)
                     /locus_tag="LR110_001841"
     CDS             complement(2378..2959)
                     /locus_tag="LR110_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4822797.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3142..3768)
                     /locus_tag="LR110_001842"
     CDS             complement(3142..3768)
                     /locus_tag="LR110_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL4822798.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3790..5121)
                     /locus_tag="LR110_001843"
     CDS             complement(3790..5121)
                     /locus_tag="LR110_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBL4822799.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5276..5677)
                     /locus_tag="LR110_001844"
     CDS             complement(5276..5677)
                     /locus_tag="LR110_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL4822800.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5763..5969)
                     /locus_tag="LR110_001845"
     CDS             complement(5763..5969)
                     /locus_tag="LR110_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822801.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6064..7566)
                     /locus_tag="LR110_001846"
     CDS             complement(6064..7566)
                     /locus_tag="LR110_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBL4822802.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7584..8039)
                     /gene="argR"
                     /locus_tag="LR110_001847"
     CDS             complement(7584..8039)
                     /gene="argR"
                     /locus_tag="LR110_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL4822803.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8137..8754)
                     /locus_tag="LR110_001848"
     CDS             complement(8137..8754)
                     /locus_tag="LR110_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBL4822804.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8975..10348
                     /locus_tag="LR110_001849"
     CDS             8975..10348
                     /locus_tag="LR110_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4822805.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10423..11535
                     /locus_tag="LR110_001850"
     CDS             10423..11535
                     /locus_tag="LR110_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBL4822806.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11607..13244)
                     /locus_tag="LR110_001851"
     CDS             complement(11607..13244)
                     /locus_tag="LR110_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBL4822807.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLLDEAKESLENLIQKLN"
     gene            complement(13412..14020)
                     /locus_tag="LR110_001852"
     CDS             complement(13412..14020)
                     /locus_tag="LR110_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL4822808.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3806 a         2953 c         2490 g         4789 t
ORIGIN      
        1 aatataaatt ctttcttctg acactataaa atttctctcc cttcaggagt taaatcttcc
       61 cattcgtctt tctcataata ctcggcctct tccccaccac taaatggatc ttcaattttt
      121 ggaactaacg taattgttcc atcatcagaa tacacaacga tatattcttg gttttctgaa
      181 ggtttctttc cgttatcaga aggtaaagtt accactacag aacttccttg taatctagat
      241 tttgtaacaa gcattatcat cactccctta ataaaagtat actatataac actaagtaac
      301 gcaaaattcc cctttgtaaa ctatctagaa aattgttttg tttaaagtat atcttattgg
      361 atataaatag aaataaattg tagccagttc attttttctt taactaaaaa aataatccaa
      421 gaaacattga acctatggat ccaaaagcta cccaaatact atagctaacc ccccgctacc
      481 ttcaattttg cagataaaac gctttgggag aaaatttcaa agacaacagg ccatcctatc
      541 atgcccgatt ataaacaaaa agctggctat tccttttgct ctgacaaatt tcaattggga
      601 aagttcacga aactagcagg tgaagaagtt tcaactgttc ggatcaaaga atgcccgctt
      661 caaatagaaa cattggttca acgaatttca actagacaag actttgctat tgtagaacgt
      721 gagatcgtag ctatttttgt agaagaaggg attttacatg atccaactca tatcaatgtg
      781 gataaatggc aacctcttat ctataaattc agagaatata caactgccaa ccagtcgtta
      841 actttgaatt ttagattcca agaattcaaa gagtcataat gataaacatc gttaggtata
      901 aataaaaaag actcgcagca cgagtaaaaa acaaatagaa gctatacatt agaaaggtcg
      961 actgtgaccc tatagatgca gtatatgcag cttatgagca tttcttctgt tctctttgtt
     1021 tttgggatct gtgctgaagt ctttttcgtt ttttgccgct ctttctttga aaaatcgatt
     1081 atgttatacc ttctccatta ctaattcagg catctgctga gtaatgattt ttcgcgcaaa
     1141 agaaatatct gttacccaag gttcgatttg ttcttcagtc aagacaagcg gcattcggtc
     1201 atggattggt gcgactgtgc ggttaggttc agtagtcatt atgattgatt ctgccatttt
     1261 ttcatcctga tggctacgat aaaaaccgcc aatatacact attttttcct tatttgaaaa
     1321 cataaatttg tctttttctt cattccactc attgggaaaa cacaacgatt ttgcaaaaat
     1381 ggttgatgaa aggtcttttt ttcttctact gtctctgcac gggcattaat catcaattgt
     1441 ccttttttaa atccggtaaa tccccacttc atgatttccg gagtgaggat gttttcttcc
     1501 tcttttctta acgtcacaac aagatttgac ggaaaaactt ctccagctgc taattctgct
     1561 gcctgcttcg gttgtttcgt ctgggcttct cgataataat tctgaaggag ctctgactct
     1621 agatcaaaaa aataacgacc acacatccta atccctccca atcttttttt ctatcataca
     1681 ctgcaacaag aagttcgcac caacaaaacg ctttcaattt ttgtagaact aaaaaagttg
     1741 tgacaagtga caaactctcc agtgaatggt attctcctgc caactcctcg gtcacaagtc
     1801 acaatattca acaaacatga gaagctgctt tttgaaattg ttccttgtga ctcggagtga
     1861 aacggcaggc ttacgacctc tgggtaaacg gtgttcaaaa gccagtttct cggtattaag
     1921 ccaaatttat gcaaaaatgt agaaagctat tttcacaaaa tttttcttaa tactcgaaac
     1981 taagcgactt tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
     2041 taagcccaac caaacgaaaa atatgagcag ctatttttat ttggctgttc ttattacttg
     2101 atgcagggca gctttttcac aacctctcca ctaaacggtg ttcaatcagc taaccttcgg
     2161 tattagctca aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact
     2221 caggtcaaag cgctgatttc acaacctcta atacacggtg ttccagaagt agcttcttcg
     2281 aaaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
     2341 tcttgaagct gaccacttct gtcacaacct ctaattatta aagaaccttt tgcgaagtta
     2401 acaatctttc tttgatcaat tgaaaattct gcctaagtag tgatagatcc tgtgtgactt
     2461 tcaacaataa gatacttccc tggatttcag caatcaacgc tttggcttga cggtctgctt
     2521 cttcatcgga cagtttttct tcagaaagtg ccaggatatt ttgtttcgta tacttgaccc
     2581 attgattgac taattctagt agcaacagac ggtattcttc atccactgta ctcaactcaa
     2641 taatcaaatt acctacagga cagccatgat aagtgatctg tgtctcatga tcagcgaaaa
     2701 tccagtccaa catttcttct aatgcttctt ctggctgttt tccagcattt aagatgtctt
     2761 cgaacaattc tgtctgccat acgtccatat gcgcatggat caccgctaga caaacttctt
     2821 tttttgaaga aaagtaataa taaaattgac cttttcctgt atcagaagct gctagaatat
     2881 ctcggattga cgtttcatga tagccttttt tataaataag atctttggtc gcttccaaga
     2941 tcttttcctt tgttttcaat gatattctcc tttttcattt ctcatagtcc tatattatac
     3001 agtaaattca tcattttttc ttcttaatcg tttaaaaaag actgtgccaa attatctgac
     3061 acagccctca ctataaaact gttctcatat agctagatgt tcattgattc aagaactgcg
     3121 aggacaaaag caattcctat cttatgatag ttgctctaat acttcctctg ttgtcatcac
     3181 ttgatctgca ataaagccaa agttcgtcaa cgctgcatcc attgcttctt ggccaggagc
     3241 accaattgca tctgtcgcta ccacaacatt gaatccactc tctaccaatt cacgcatatg
     3301 ggaatcgcta cacaagtttc cgttcattcc accaagaatt actgtgtcga ttccattttt
     3361 tctcaattgg agcgccaaat cattgctttc tggtccaaag attttatgag gagagctgat
     3421 gatcgtattt tcatccaaat aaggtttcaa ttcttcgata aaatcggctc ctgaaccttc
     3481 ttccactgat tcatattgac tttttctagc aaacatctgc tgctcaagca tcatatgttc
     3541 tcctgcaccg ccaaattgcc atttatggtc atgtgggtaa aaataatgag gagaaataaa
     3601 gagtttgtat cctttttctt ttgcttgttt taccatttca atcatatttt cgatcgtatt
     3661 gtatttttct aagatatctt ttgtcaaacc aaagccaccg cctgctggtt tcaagaactc
     3721 gacttgcggg tcagtgagaa ctactgctgt atttttatta ttgaacatgg tggattctcc
     3781 attcgttatt tattttaata catcatttag agcttcactc attgtcggat gagaatagat
     3841 ttgatctctc aataacgtat aaggaagttt tccattcata gctagcgaaa tcaagttgat
     3901 gacttcatga gaatcttccg catagatact tgcccctaga atcaagtcag tttcagggtc
     3961 gactaaaact ttgaatacac ctttaggatc ttctaatact tgtgctttag ggacaccgct
     4021 tgtcatatac ttgaataatt tgtaattgac gccttgtgct ttggcttgtt tttctgttaa
     4081 acctacattg gataaagtcg gagtgataaa tacactgtaa ggcacagctt tccggttatt
     4141 cgttgttcgt ttattctcgc ccttcaactg atccataatg attcgataat catctagaga
     4201 gatataagtg aactgcaatc cgcctttgac gtctccaatc gcccaaatgt tgtctgcatc
     4261 tgtacgaagg tattcatcca cagcgattgc tccgcgatct gttagtttaa catctgtatt
     4321 ttctaatcca agtgatgctg tacttggttt tcttccagtt gcagccaaaa cttttgacgc
     4381 ttggattgtt acttcttggc catcttctgt aaaggtgacg gcagctgttg attcttggtc
     4441 tgctacttga tctacagata cacctagatg gaattcgatt cccgcatttg tcatgtcttc
     4501 taaaatcatt tgcgcgatat cttcatcttc acgtggaaga aattcagaat gggcatcaag
     4561 aacgacaact ttagatccgt attcattgaa cattgatgca aattccaagc caatatatcc
     4621 agccccgatg atcactaatt tttccggtaa ttgttcctga tccatcgctt gcgtagaatc
     4681 aatcaagtag cgacttgtat tcaatccttt gattggtaaa atgattggtt cagaaccagt
     4741 attgatgaaa atccgttcgc cttcaacaat cactttttca ccattattcg tcacttcgat
     4801 ttgatggtta gaaagaaaac gtgctgtacc gtccatcact gtcccagttg cctcatcgct
     4861 gatcatatga taatttttgg cgcgaagcat gcccgtcaat ttttctttcc gagcaacagc
     4921 ctctgtaaag tcgattcctc tttctccatt gaaaatcaat gatttagaag gaatacagcc
     4981 gatgttgata catgttccgc catacatacg tgttgacttt tcaacgacta aaaccgattc
     5041 accttttgaa gctaacgcct tcgctaaagt cttaccgccc tttccaaaac cgatcacaat
     5101 attttgatat ttttccatca tttttccctc gcttcttttt tacctaccga ttggtacaaa
     5161 aatatgataa ccgataattg tctattctcc aaatcatctg ctttatttgt caaaaaaaac
     5221 cagacaaaaa gcggtccttt cacgttttta gtctggttcg atttccttta ttcttctaaa
     5281 caatcgttac ttctacatcg atatttcctc tagttgcttt ggagtaaggg cagaattcat
     5341 gcgcatcttc tgcaattttt tgggcttctt cttcgctgat gccatcgata tgcacttcta
     5401 atactacacc gatctgatag ccgccatcat ctaaattata aagagagaca cgtgctttta
     5461 ctgtggactc tgctgtcatt cctgcttctt tgatcatatg ttgtaccgcc ccattgaaac
     5521 aagaagcata acctgctgca aataattgtt ctggatttgt tccttctgca tggattcctg
     5581 gaggagtgat tttcatatgc atcgagccat ttggtgcctc gacttctcca ttgcgcccgc
     5641 catggttgat cattgctgtt tcgtatgctt ttttcattgt actgctcctt tcgattgacc
     5701 atttgctact tggctataat tatagtgtat gacaagtaag gcgctttcta caaaaattct
     5761 gcttattcga ataccagaat gccgtcttct actatttttc caaaaaacaa aaaaatacta
     5821 ctcgtgaaca acagcgtcat atttgttcca aaataaatag caatcttttc tacataatct
     5881 tttatatgat tgattttaag atacatatat accagaaagt tgaccccata aaccaatact
     5941 gttaaaaaaa taaccatatt cgttaccatt ttttcctcct tttgctggtc tccctaccag
     6001 cactcgattt aaccatacca ctttttttgc cgtccaagca actagccttt caaaaagact
     6061 ggtttattct gctgttaatg attcaagatc agaaaaatgt ccgaataatt ttgctaattt
     6121 tactaacgcc aacttgttct ttccgaattc atcattttct atctgtaata ctagtaacgg
     6181 ctcatgtaag cttaaacgga tcaaaaacca gccgtttccg tatggctctt ctagatcata
     6241 tcgaatacct tcttcatttt ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg
     6301 cttcatttct tctaatatct tgtttccttg ttcgcgtggt tcatcgctga acaattttaa
     6361 tcggatttct tgtacttcta cgggctgttt caactctttg atttgttcat ctaatgtgat
     6421 accttttctt tttaattctg gcaaaagcat caggatcttt gcgatgacat atgcaccatc
     6481 atccagaaaa tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt
     6541 atctttccca agacggttaa gttctaatgc tttattgatt acatttcgat agccggaaat
     6601 ataacgaact tggatgcctc ctttttcttc aatgaatctt tgtagatgag aagaagtagg
     6661 ggaattcgtc acgattcttg ttcctggatg ctcttttaat acgattgtac ttaatactgc
     6721 gatcaaatta tttcgattga tcactgttcc agaagcactg acaactgcag aacgatccac
     6781 atctgtatcg aaaatgatcc caagatctgc ttcatttgct aaaacagcct gcttgatact
     6841 ttccatcgct tctttattat caggatttgg gatatgatta ggaaatcggc catctggttc
     6901 caaaaattgg gaacctgttg aatccgcacc taagctattc aatacttttt cagcaaagaa
     6961 tccaccagca ccatttccgg catcaacaat gattttccaa ccatttagtg gtttgctttc
     7021 tagtgttcca atcccctttt taatttttgt gactaaatct tgcgcataag gtgtcaaaag
     7081 gtctgctttt tctacgcttc cagtttgttc gctttttatt ttttgctgct gtgataggat
     7141 aaaagcaata tcttcttttt ctgctcctcc ttctgaggta aagaatttta ttccattata
     7201 ataataggga agatggctag cagtcagcat aaccgcagca tcgcacgaga actgttcaaa
     7261 ttgagtggac ataaacatag caggcgtagt agctaattcg aaatcgatga catgtatccc
     7321 ttgttctttc agactttgga tcaacgcctc tttcagtgct ggtccgctta tcctactgtc
     7381 atgaccaatc ccaatcgtcc attttttatt tggatcggtt gatttcttct ttttctttat
     7441 ccagtttgct agtccccagc cgatcttttt taccatgtct gtcgtcaagt ttacttcata
     7501 tccttcaact ggtaatgcga ttcctcgaat atccgatcca ttttgcagcg ctttcatatc
     7561 tttcatataa aagtcctcct catttattcc ctcatttttt ccagcttttc tgcaaatcgc
     7621 tgtgcttctt cttctgagcg gcaaataata atgaatgtat ctacaccggc catcgttccg
     7681 acaacttcag ggtaagccac ttcatctagc caattcgtta ccacatcggc accatttcgt
     7741 tcagtcaata cgataaccat gaactgaaca gactggatac gcaaaacctc gcgtctaatt
     7801 gctgtacgca aacgttcttc atttaaagaa acagatggct ggttaaataa tgtatagcgt
     7861 actattttgt tttcattttg actttttact aacttcattt ctcgaatgtc tcgagaaatc
     7921 gttgcttgag tcacatcggc gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc
     7981 ccaataggtt gttctgtaat gatctggcgg atcagtgctt gtcgctgttt ctttttcata
     8041 ttagacacct ctcaatgttg attatagcaa aagataagta atatttcttc atattttggt
     8101 attttttact tttatttttt gttttcattc aggttgctaa cctttttcta ttttaacacg
     8161 caaaatcgtc ttcatttttt caggtgctga ttttcttgga tcgctgatgt aaatctcttt
     8221 gtgttcttta gaagctcttt catagccttc ttttgcgatg aatgcttcta atttctcaaa
     8281 cgttgcaggt tcttcggcat agggcccgac gtgtaagatt tgggcacaaa gaccttcttc
     8341 catctttttg aatactactt gttctttcaa gtcttccgga agtttgctag aacgctgaat
     8401 ggcttcttgt gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca attgataaga
     8461 aaaatgttct tttttcatca ctggttcatg caaatacttt tcttgcagtc cccattgtcc
     8521 ttccagtgga tagacagtat acatctgata atcaggaatc agccaatttt ttttaggcga
     8581 catgcgtatt gcatagctga ctgcatatag acaggctact cttcgtttaa agtcttcctg
     8641 attcgggtcg ccgatccctc tgatgctaaa gaatggtttt ttaggaacct ctagtatttg
     8701 cgggcttttt ttcatactat agctcttgtc tttacgccat tcaatttttt ccatttttct
     8761 tcctccgcga aattccattt agttatttat tatcatacct ctttttgagt gacttggcta
     8821 atcgtgttga cactattttt cagtttgtgc tagaataagt gaagtaaata aatcgtacag
     8881 ataacacgtt tcggcgtgtc gagagagagg cggtttctat ttttataata gaaatcactc
     8941 tcttttttta cgaaattttt tagaggtgat gtaaatgaca aaacaaaaac agctgctttt
     9001 agtacctgca gtaattgcaa cagggatgat gtcttttgcg ggcgtattga tcgagacggc
     9061 catgaatgta actttcccaa ctcttatcag acagtttgat ttaacgactg cacaagtcca
     9121 atgggtaaca acgatttatc ttttgatgat ttcgatcatt gttccgctgt ctacttattt
     9181 gaatcgcaat tttagtctca aaaaactctt tttagcttca aatctgcttt tcttggcagg
     9241 tataatgatc gatttcttct ctcctacctt catggtccta ttgtttggtc gattgctcca
     9301 aggtgcagcg acaggtatcg ctcttccgtt gatgttccat attatcttga catttgcccc
     9361 cctcaatcga cgaggcgcga tgatgggtgt cggtactttg acaacggcca ttgctccagc
     9421 aattggcccc acttatggag gcattatgac ttcacatttt acatggaatc atatcttcct
     9481 gtttcttgtg cccgttttga ttctttcttt gattatcggc tggtttgcta ttcccgagat
     9541 ggcagtacaa aaaagtggtt atttggattg gataagcgtt ttaggaattt gtcttttgtt
     9601 tagtggaagt ctaacatttt tgagtacgtt gggaacagtt tccagctgga ttgctttaat
     9661 catcggcggt attggctggt tcctttttta ctatcgaagt aaaaaagtgg agcatccact
     9721 gatccgactg tctattcttt caaataaaac attccgcttg tttttgattg gcttcttagt
     9781 ttatcagttt ttattacttg gtgtatcttt tgtcttgccg aatttccttc agatcgttca
     9841 aggaagtagt ccttttgtcg ctggattagc gatgcttccc ggcgcagcgg ttggtgcttt
     9901 gctttctcct ttttccggga aaatgctgga tcaatatggt ccgaagaagc caatttttgc
     9961 tgggctgatt ctttctttga ttggttggct agcattattc ctactcatag gacacgcttc
    10021 agtgggtgta ttgattgctt gtcatgtgtt ttatatgatc ggtatcggat tatcttacag
    10081 caatatgatg accaccggaa tgaatgcact gaatgaagaa ctacaaggag acgggaacgc
    10141 aatgttcaat accttgcagc agttttctgg cgctgtggct acttcattag tagcggtcat
    10201 tatcaattac gtccagcatc atacttccca taactatgaa gtgtctacta cattaggatc
    10261 aaaagcagca ttaggcgtct tgttgctgtt gcttctcgta agctttgctc gattcgccta
    10321 ttatctgttc tttgctaaaa aagcgtagat tccatatacg gcaggtgttg catctgccgt
    10381 ttttttgtgt gaaaatggtt gtttgtaaaa ggaggctttt ttatggaaaa aattgccatt
    10441 atcggtggcg ggatcattgg tatgacccta gccagttact tagatacatc aaaattcaat
    10501 gttgctttat ttgatgaagg gacaggacag gcaactaaag ctagcgctgg gattatctcc
    10561 ccttggttgt cgaaacgcag aaacaaacaa tggtatcgtc tggctaaaga cggcgctgct
    10621 tttttcccta aacttataaa agatttccaa cttgatcaaa cgatttacgc acaatcaggg
    10681 acacttttgc ttcgacctga aaaagattta gctgaattgg ctgttcttgc cgagaaacga
    10741 aaagaagaag cgccagaaat tggaaacatc gaatggcttt cacctgaaca gacatctcaa
    10801 gcacttccgt tattgaaacc agcagtttca ctgcgtatca gtggtggtgg tcgtctggat
    10861 ggaaaagcat atttagaaca tcttggtcag cgtttagcac aaaaacaaat ggagcagatc
    10921 caggcaaaag tcactttgca taagaagagc gattcttggc tcatcaaggg accgtttgga
    10981 gaatacacag ctgatcgtgt aattttgaca cctggcccgg cacttaagcc tttattacaa
    11041 gaattaggct atgctgcaga tatacgtccg caaaaagggc agctcttagc atttcagaca
    11101 tcttataaga tgagtcaaga ctggcctgtt gctatgcttg acggagaagc tgatttcatt
    11161 cctttcatgg atggaaagat ccttttaggt gctacccatg aaaacgatca aggctgggat
    11221 ttggctccta ctagcgaagc ttataaggga ttgactaaga gtacagaaca gttcttgact
    11281 gatccagatc agataaaaaa ctggccatac gagttccgtg taggtacccg tgcttatacg
    11341 tcagattttt ccccattttt tggcgtactg ccagatgatc caagcttact ggctgctagc
    11401 ggtctaggct cttctggctt gaccacaggt ccttatatcg gctatctatt agccaacttc
    11461 ttaaatactg gtcattggtc tacggaagat tatcaaaagg acatctccac ctatattacg
    11521 aaaaatccat catagaaaaa cagctttttc aagtaaggat cccgagcgat attgcgcatg
    11581 gattacttct tgaaaaagct gttattttag ttcaatttct gaatcaaatt ctccaaactt
    11641 tcttttgctt catctagcaa gagatctttt cctatctgag cttcaatgaa tcgtaaacga
    11701 atatcaagtc gttgtttcac cagattttta taggacgtat cctcaaaaga aacaggaaaa
    11761 tctggtatag gtaaatagtg gatgtcttca aatggaccaa acgacttgaa ttctgccgtt
    11821 tgttgcacga ttctttcaac gattgacaat gtcgtttcag gtttgatttt tttatcatcg
    11881 aaatatccag tattcaggac atagcaggct gtttgctgtt tcaaaaacaa ctctcgaaaa
    11941 gcttgatagt cttccctaag aggatagaca cggaaaggat tagcaaatgg ttcaatgact
    12001 agctcatcta gctgtctttt tgtagtgata ttttccgcgt ctgatcgttt tgttgctaat
    12061 gttgcaccaa aagacgctgc taaaactggg tcattgatct tgataattgg cggaagcgaa
    12121 tcatctttca tgatccaaaa aacagcatcg atcttttcgc taagatgatc gactcggtcg
    12181 ggtgtcgcat aacgtgattt gactgtccgc ccatttccgt ttcgtatatc tcctgttact
    12241 aggacttttt ctcctttact tgtacatgtc acgcccacat tctgacaagt aaggaaagaa
    12301 gaaatggccg gatcatcaga cggatagtct tgtgttttat caaagtaaga aggttccaag
    12361 gcagtagcaa aaccattttt tcgagaaata acgaaagcat cgtcatgcaa gatcgaaaca
    12421 gagtgacctt ccgtatgttt tgctaaggta atcgttgatt ttccagaacc ggacaaccca
    12481 aatacagcca tcgtataggt ctggtcatta ctagcatatt gtttcatccc accgtggcag
    12541 gcaataaagc cattgcgatt tcccaatgcc caagcaagcg tcaaagtcgc tttttttaat
    12601 tctccaaagt aacgtaatcc tagcacagcc gctgtattat ggagagcatc aaacaaaatg
    12661 agtccatttg gaaatgtttc atgtgtccat tcgggatcac aaaagatata aagatctccc
    12721 tcatcaaagg aaacagactt ttgataagtc tctaagatct tcgaattcgc tggctgaaaa
    12781 ttcagcaaat aagaatacaa attgttctca aatccttctg gaatcagcaa atgactttgc
    12841 acagaaaatt cttcagatag gccaacgatt acttttgtat ggtaaaactg gcgcttgcga
    12901 ctttgataca cagcttctct taaaattttc tcgtatttgc tttgttcctt gtctaagtcc
    12961 tcaaagatcc gccgagcact tgctgttctt cccacaatgc ttccatcatt gaatactagt
    13021 gtcctaacat cagctggtaa atctagttct tcagcatgtg agattgttaa atctgtgatg
    13081 atcgttcctg gactcccttt tgctagttga taagctgtct tcctgtcaaa aacttcatga
    13141 acattattac cgtaaaatgt cgtttctaca atcgatcgaa tactggataa cagtggattt
    13201 ccacttgtga tttcgtttaa ttcgagactc tcttttgtac tcatggctcc tcctcattcc
    13261 ctaaacaaaa aaacgttttc tttattatac attattcgag tttttatttg tactgctgcg
    13321 ctgtgggaag caaacgagtt atttttcaca caaaaaaacc gagaacttga ataaacaagt
    13381 ttctcggttt catcattttc cttttatcgg cttagtacca gccgtttgct aaccagaatg
    13441 ttttagcagc atcccatgaa ccataacgag atgttacata ttgttctgct acacgttctt
    13501 gattggcagc tgaatagtcg ccgtttaagt atgaagcatc taattgataa cggccgatgt
    13561 aacgtccgtt agtagctgtg tatgaaccgc ctgattcttt ttgtgcgatc cattcttttg
    13621 cagagcttga agttggcgct gttgcagcag ctggggcagc aggagctact tcaactgttt
    13681 caacaggtgc aacgtatgta tgttcttgcg ttgctgtttc agttgtagat gctgtatttt
    13741 ctgttgtttt gttttctgtt gttgctgaag aatcatttga agttgggatt gttaattgtt
    13801 caccaacata aatgcggttg atgtttgcga tcgaattttt ttcagcaatt gcatcgatca
    13861 ttgagttgtc tcctgcaaat ttttgtgaga tcttagataa tgaatcacct gattttactg
    13921 tataaacttc atcagcgtga gcatttgtag ttcctacaaa cattgcagta gcaccagcag
    13981 caaaagttgt acctaaaaca attttcttaa atgaattcat attaaactcc tttttttc
//
LOCUS       DAFFSV010000071        13959 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFFSV010000071 DAFFSV010000000
VERSION     DAFFSV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.071"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..135
                     /locus_tag="LR110_001853"
     CDS             <1..135
                     /locus_tag="LR110_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL4822809.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="LR110_001854"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="LR110_001854"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBL4822810.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="LR110_001855"
     CDS             complement(2412..2663)
                     /locus_tag="LR110_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBL4822811.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="LR110_001856"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="LR110_001856"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBL4822812.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="LR110_001857"
     CDS             3613..3831
                     /locus_tag="LR110_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822813.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="LR110_001858"
     CDS             complement(4120..4632)
                     /locus_tag="LR110_001858"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBL4822814.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="LR110_001859"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="LR110_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBL4822815.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="LR110_001860"
     CDS             complement(7085..7528)
                     /locus_tag="LR110_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBL4822816.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="LR110_001861"
     CDS             complement(7529..8317)
                     /locus_tag="LR110_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL4822817.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /locus_tag="LR110_001862"
     CDS             complement(8314..9255)
                     /locus_tag="LR110_001862"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBL4822818.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="LR110_001863"
     CDS             complement(9432..10019)
                     /locus_tag="LR110_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBL4822819.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="LR110_001864"
     CDS             complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="LR110_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBL4822820.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="LR110_001865"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="LR110_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL4822821.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="LR110_001866"
     CDS             complement(12936..13871)
                     /locus_tag="LR110_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL4822822.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3863 a         3126 c         2454 g         4516 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAFFSV010000072        13889 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFFSV010000072 DAFFSV010000000
VERSION     DAFFSV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.072"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            153..1196
                     /gene="hrcA"
                     /locus_tag="LR110_001867"
     CDS             153..1196
                     /gene="hrcA"
                     /locus_tag="LR110_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBL4822823.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            1290..1853
                     /gene="grpE"
                     /locus_tag="LR110_001868"
     CDS             1290..1853
                     /gene="grpE"
                     /locus_tag="LR110_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBL4822824.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            1904..3733
                     /gene="dnaK"
                     /locus_tag="LR110_001869"
     CDS             1904..3733
                     /gene="dnaK"
                     /locus_tag="LR110_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBL4822825.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            3884..5050
                     /gene="dnaJ"
                     /locus_tag="LR110_001870"
     CDS             3884..5050
                     /gene="dnaJ"
                     /locus_tag="LR110_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBL4822826.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(5132..5797)
                     /locus_tag="LR110_001871"
     CDS             complement(5132..5797)
                     /locus_tag="LR110_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4822827.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            5773..5946
                     /locus_tag="LR110_001872"
     CDS             5773..5946
                     /locus_tag="LR110_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822828.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(5991..6728)
                     /locus_tag="LR110_001873"
     CDS             complement(5991..6728)
                     /locus_tag="LR110_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL4822829.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(6730..8232)
                     /locus_tag="LR110_001874"
     CDS             complement(6730..8232)
                     /locus_tag="LR110_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBL4822830.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            8491..10485
                     /gene="uvrB"
                     /locus_tag="LR110_001875"
     CDS             8491..10485
                     /gene="uvrB"
                     /locus_tag="LR110_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBL4822831.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            10495..13311
                     /gene="uvrA"
                     /locus_tag="LR110_001876"
     CDS             10495..13311
                     /gene="uvrA"
                     /locus_tag="LR110_001876"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBL4822832.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            13574..>13889
                     /locus_tag="LR110_001877"
     CDS             13574..>13889
                     /locus_tag="LR110_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822833.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQE"
BASE COUNT         4526 a         2476 c         3051 g         3836 t
ORIGIN      
        1 aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg
       61 gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgga ttagcactta
      121 gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt
      181 tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattga
      241 tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag
      301 atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct
      361 atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc
      421 tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg
      481 ctgagatatt atctgagctg acaagctata cgactttttc attgggacct gaaataaaag
      541 accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg
      601 tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa
      661 gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga
      721 tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaaa
      781 cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagataagg
      841 tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt
      901 tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg atcactccga
      961 tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata
     1021 tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt
     1081 tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag
     1141 aactagcagc taaacttgct gattactacc gttcgcttga cttgtcaaac tcgtaatgtg
     1201 aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc
     1261 tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag
     1321 aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta
     1381 tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa
     1441 tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa
     1501 atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat
     1561 caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt
     1621 tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa
     1681 ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa
     1741 ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct
     1801 acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg
     1861 gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg
     1921 tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat
     1981 catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga
     2041 aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc
     2101 aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac
     2161 accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt
     2221 aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg
     2281 tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga
     2341 accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt
     2401 agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt
     2461 ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa
     2521 aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga
     2581 caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg
     2641 cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca
     2701 tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg
     2761 tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat cagaaatcga
     2821 cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa
     2881 agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc
     2941 tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac
     3001 accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa
     3061 tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc
     3121 agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg
     3181 aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac
     3241 attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa
     3301 agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat
     3361 ggtaaaagat gcagaagcaa atgcagaagc agataaagct cgtaaagagg aagttgacct
     3421 acgtaatgat gtagatgcat tgctattctc tgtggacaaa acattgaaag aattagaagg
     3481 caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc
     3541 tgttgaagca aacaacatcg aagaaatgaa aactaaacgc gatgcattga acgaaatcgt
     3601 tcaaaacttg actgttaaac tatacgaaca agcggcacaa caacaagctc aagaaaaccc
     3661 agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg
     3721 cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt
     3781 tttcttttga aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata
     3841 acaagcgacg tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga
     3901 ttattatgaa gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta
     3961 tcgtaagctt tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa
     4021 attcaaagaa gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata
     4081 tgaccaatac gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg
     4141 atttggtgga ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga
     4201 ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga
     4261 tttgcaatat acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat
     4321 caagtataat cgggaagaaa tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac
     4381 acaaccaact acttgtcata aatgtcatgg tgcaggtacg attaatgtag aacgccaaac
     4441 accacttgga cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga
     4501 aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt
     4561 taaagtgaat gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg
     4621 agaagctgga gaaaacggcg gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga
     4681 aagtgatatc tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt
     4741 acaagcagct ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa
     4801 gatccctgct ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa
     4861 gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa
     4921 tctaaatgaa caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt
     4981 tgaagaacag caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa
     5041 gcggaagtag tttttctagt aaaaacgcta agtcattaat aattaaaaac cagacgaaca
     5101 atcatatgtt cgcctggttt ttttgtagtt gtcatgtttg taccaacaaa aaagccagct
     5161 ttagaatatg ataatcgtca tagtttctag gggtataacc agtgagagaa tggaacttgt
     5221 tcaatcgata ttgcaaggtg tttttatgaa taaataattc ttctgagcaa tttttgatgc
     5281 ttccgttatt tttactgaag catgccaaag tcttcttgaa caaatgtact tcttcttctg
     5341 tcaggctttt caaaatacgt tttacatata atgttcgttg atctgacggg attgctggta
     5401 aaacgatccc gaggtctaaa tcttcaaaaa agcggatctc gttcacagat tgatggaaag
     5461 ctatccattc gcaaacttgt ttggcatgaa ggaaagattg cggtgtctct tgctctgtat
     5521 aacaaagcga cccaattcca aagtttagct gtaactgata cattttatta aactttgtct
     5581 ggattttatc aagcaatgat agcaatgagg ctctggaaat ccgattgact aggataatga
     5641 tttgttgtct atgtacagtg agcaaagcct gtccgtaata agagaagacc ctttcgagca
     5701 actccaataa aggattgatc cgttcttgac acaattgctg attgtcagaa agttgtccca
     5761 caactacata atatggagaa gaaaaatcaa gctcaattaa gtgagggtgg tatcgactat
     5821 gtacttgata tcatcaatga accgatggta ctttcttatg cgatggaaca ggtagagcga
     5881 ttgcttgttt gtgcaggaaa acaagcgatg cggctgatac gattaggtga gaaaataaga
     5941 agataaaaat acacggtccc ttcatggcga ccgtgcattt ttggttttta ttaaatattt
     6001 aatactttat tcaagaaatc ttttgtccgt tcattttgcg gattttcaaa gatttgttga
     6061 ggcgttccat cttctaaaaa gtttccgtcg tcaatgaaca tgacgcgatt ggccacttct
     6121 ttagcgaacc ccatttcgtg cgtaacgata accatcgtca taccttgttc tgctaacttt
     6181 ttcatgacgt tcaagacatc accgaccatt tcaggatcga gtgcagaagt aggctcatca
     6241 aaaagcatga tgtcagggtt catcgctaga gcacgagcaa tagccacacg ctgtttttgt
     6301 ccaccagaaa gcatttcagg atacatatct tttttatctt ctagaccaac tgtctgcagt
     6361 aatttgattg cttttttctc tgcttcttct ctagattctt tcttcaggtc catcggtgct
     6421 aaaatgatgt tttccaaaat agaaagatga gggaataggt taaagtgttg gaataccatt
     6481 ccgatatgtt gtctcaccaa gttgatatct gtgcttttat ccatcaagtg ggcgccgtca
     6541 ataataattt caccgtcaga aggttcttct aaacgattca agcaacgcaa gaatgtactt
     6601 tttcctgaac ctgaagggcc aatgatacag acgacatctc cttcgttgat ggatacattg
     6661 atgtcgttta atactgtgtt atcgccgtat tttttgacta aatgctcaac taagattttt
     6721 tctgccatct tacttcacct tcttttctaa aacttttgct aattttgtca atgctgtgat
     6781 caaaatcaag tacatgatcg caatgatcaa gtaaacatac gtactttgtg ttgtacgtgc
     6841 aacaatgatc ttaccagttt gcagcaattc gactacaccg attgcggaaa tgatcgttgt
     6901 gtcttttaat gagataacga actgattgac gaatgatggg atcatgatgc gtatcgcttg
     6961 cggcaaaatg attttttgca ttgtccgatt ataagaaaga cctaaacttc tggaagcttc
     7021 catttgtccg acaggaacgg cgttgatccc gcctcgaaca atctctgcga tataagcact
     7081 tgcgttaaga gtcaaggtga taacaccagc aaggaagtca ggaatcgtaa atccaacgat
     7141 accaggcaac ccaaagaaaa taaagaacgc aagaaccatc attggaattc ctcggatgat
     7201 atcgacataa atactagcaa ttgtgcgaag agcgcgtatt ggtgctacac tgaataatcc
     7261 aaaaatcaca ccgattacta tagccaaagc aaaggagatc aaagttagga gaattgttct
     7321 ccaaagtcct ttcaacaatg cttgatagtt gttttggata agtccaagaa atgttgattc
     7381 atcagcagtg gaagactcac tggaagtttc tgaaccatcc gctacatatt ctttaataat
     7441 cttatcgtat tcgcctgttc tttttaattc tttcaatcct tcattgaaca tattaagaag
     7501 ctcaggattt tgtccttttt taacggcaaa tgcatatttt ccgccagatt cttttttgat
     7561 cggtgtttcc aattcttgtc cttgtttgat cgcataaccg attaccggat aatcatccat
     7621 cattgcatcg attgcgccaa cttttaaccc gtcgtacaat tgatcagcag catcaaaata
     7681 tttgatagaa tagccatatt cagattcatg gtcgcttaaa aattcagcac tttcggtacc
     7741 aacttttgct ccgactgttt ttcctttcaa atcttcataa gacttgatcg aagtatctcc
     7801 ttttttgaca gctaattgga tcccgctatc aaagtaagaa tcagaaaaat catagctttt
     7861 ctttcgttca tctgttacag tcattccggc aatcattccg tctgcttgtc ctgattccaa
     7921 agcttgcaaa gcagcagaga aaccacggta gtccattcgg atgttaaatc cttgcagttt
     7981 ggcgatgttt tccatcaatt ttacatcgat gccttcgtac tcgccctttg cgttttgaaa
     8041 ttcaaatgga gcaaaagcag agtcactggc aattacgtat acgtcttttt tgggctctat
     8101 tttttgcatt gtctcgcttg tgcttgactc tgccgttgta gtgttgtcag cttgtgcaac
     8161 atgaccaaac gcaagtgtca caagtcctgt cattaagaaa acaaatgagt gaaataaatg
     8221 ctttcttttc ataatcaacc tcctaaattt ccaatatttt attattatag gcgaaaagtg
     8281 ctgaatcaga caagactgta agctcgaaaa aatgaaaaaa atgcgagttt ctaacatttt
     8341 atgttataaa acataacatg tataataaga taaaaaagtg gcgaaagtat gttcggtgtg
     8401 gtataatttt ttagtaagga atgcctttgc tttccttttc tttttattgg tcgtaaaatg
     8461 aaaaggaatg aatgaaaagg aggacgaaga atgattgaga gagatacctc ccgacacttt
     8521 gagcttgtct cgaaatacca accggctggt gatcagcctg aagcgattaa ccaattagtg
     8581 gatggcgtag tcggcgggaa aaaagcacaa atcttattag gtgcgactgg aacagggaaa
     8641 acatatacga tttccaatct tattgagaaa gtgaataaac caacactgat tattgcccat
     8701 aacaaaacat tggctggtca gctgtacggg gaattcaagg aatttttccc taacaatgct
     8761 gttgaatatt ttgtttctta ttatgattat tatcagcctg aagcttatgt gccttcaagc
     8821 gatacgtata tcgaaaaaga ttcaagtgta aatgatgaga tcgacaaatt acgacattcg
     8881 gctactagtt cattattaga aagaaatgat gtgatcgtca ttgcatccgt ttcatgtatt
     8941 tttggattag gctcgccttt tgagtaccaa aaacaagtag tttcgattcg acagggagca
     9001 gaactagatc gcaatcagtt gattcgtgac ttagtcagta tccaattcga aagaaatgat
     9061 atagattttc agcgcggtcg ttttcgagtg cgcggtgatg tcgtggagat ttttccggct
     9121 tcaagagatg aacgtgcctt acgtgtggag ttttttggag atgaggtcga acgtatccga
     9181 gaagtcaatg cactgacagg tgaagtttta ggagaaacgg aacacgtagc aatcttcccg
     9241 gcgactcact ttgtgacgaa tgatgaacat atggagcatg ccgtagcgaa tatcaaagag
     9301 gaattagaac agcgtttaac tgtcttgcga aatgaaaaca aacttctgga agctcaacgt
     9361 ttagaacagc gaaccaatta cgatattgaa atgatgttag aaatgggcta cacgtcggga
     9421 attgaaaatt attctcggca tatggacgga cgtaaagaag gtgaaccgcc ttatacgcta
     9481 ttggatttct ttcctgaaga ttttttgatc gtggcggacg aatcgcacgt aaccatgcct
     9541 caaatcagag gaatgtataa tggtgaccga gcgagaaaac agatgctagt agattacggt
     9601 ttccgtttgc catctgcttt agacaatcgg ccacttcggt tagaagagtt tgaaaaacat
     9661 gtcaatcaga tcatttatgt ctctgcgacg ccaggaccat atgaacatga acaaacagat
     9721 accgttatcc aacaaatcat ccgtccgaca gggctgcttg acccagtgat cgaagttcgt
     9781 ccaatcatgg gacaaatcga tgatctagta ggcgaaataa atgaacgagt cgaaaaagac
     9841 cagcgggtgt ttgtaacgac attgacgaaa aaaatggcag aagacctcac agattatttc
     9901 aaagaattag ggatcaaagt caaatatttg catagtgata tcaaaacgct ggaacgaaca
     9961 gaaatcattc gagatcttcg attaggtgaa tttgatgtgt tagttggaat caatttgctt
    10021 cgtgaaggat tagacgtacc tgaagtttcc cttgttgcta tcttggatgc ggataaagaa
    10081 ggctttttac gtagtgaacg ttccttagtc caaacgattg gacgagctgc tcggaatgaa
    10141 gaaggtaaag taatcatgta cgcagacaaa gtaacagatt ctatgcgtct agcgatggat
    10201 gaaacgtcaa gacgtcgtac gattcagcag aagtacaatg aagaacatgg gatcgttcca
    10261 aaaacgatca ttaaagaaat ccgagatttg atttctatta cgaaagaatc ggaagatgat
    10321 acaaaagaag cggtacaagt ctcgtacgaa gaaatgacga aggaagaaaa agacacgtta
    10381 ttgatgaaat tagaaaaaga aatgaaagat gcggcaaaag ctttggattt tgaaacagcc
    10441 gcgaatgtgc gagatatgat tttagaatta aaagcttcaa agtaggagga caacatggct
    10501 aacgataaaa ttacgattca tggtgcacgt gcccataact tgaaaaatat agatgtaacg
    10561 attccacgag ataaattcgt ggtcgttaca ggtttgtccg gttctggaaa aagttcccta
    10621 gcatttgata cgctgtacgc tgaagggcag cggagatacg tagagagcct ttctgcttat
    10681 gctcgtcagt ttttaggaca aatggataag ccggacgtag atagcatcga cggattgagt
    10741 ccggcgattt ctattgatca gaaaacaaca agtaaaaatc ctcggtcgac agtaggtact
    10801 gtgacggaaa tcaatgacta tttacgcttg ttgtttgcac gtgtaggaca tccgatttgt
    10861 cccaacgatc atatcgagat taccagtcaa tcagtagatc aaatggcaga tcaagtaatg
    10921 gctttgcctg agcgcacaaa gatccaagtg ttggcaccga tcgtggtaaa gaaaaaagga
    10981 caacataaaa aaatattcga acgaatccaa aaagagggat acgtccgcgt tcgagtcgat
    11041 ggtgagatct atgatttaag tgaagtgcct gagctggaga aaaacaaaaa acatgatatc
    11101 gcgatcgtca tcgaccggat cattgtgaaa gaagggattc gttctcgcct atttgattct
    11161 ttagaggcgg ccctgcgatt agcagacggc tatgctctgg ttgacgtgat cggtgaagaa
    11221 gaaatgttgt ttagcgaaca ctatgcctgt ccttattgcg gatttactgt tggcgaacta
    11281 gaaccaagac tgttctcatt caatgcgcct tttggagctt gtccagactg tgatggttta
    11341 ggtgtcaaat tagaagtcga tcaagattta gtgattccag atagaacgaa gacacttagt
    11401 caaggtgcta tcgttccatg gaatccaatc agctctcagt attatcctca aatgttagcg
    11461 caggcttgtg aaagtttcgg tatcgattta gatacaccat tcgaagaatt accggaagag
    11521 catcaggaga tcatcttgaa tgggacagat gatcttttcc atttccatta tgaaaatgat
    11581 tttggtggcg ttcgcgatgt agaaacgact ttcgaagggg tattgaagaa tatcgagcgt
    11641 cgttatcatg atacgaacag tgatttcacg cgtgagcaaa tgcgtcttta tatgacagaa
    11701 ttaacttgtc agacatgtaa aggttatcga ttgaatcccc aagctctatc tgttcaaatc
    11761 aatggaacga atatcgggca aacaaatgag ttgtctattc aaaaagcagt agatttcttc
    11821 tcaaacttaa cgttatctga acaagagaaa gtaattgcca aaccgatttt aaaagaagta
    11881 aatgacagac tttcattctt ggaaaatgtg ggactagact atctaacttt gagtcgcgct
    11941 tccggaacat tatctggggg agaagcgcag cgaatcagat tggctacaca aattggatcg
    12001 aatctttctg gagttttgta tattttagat gaaccatcga tcggattgca tcaacgcgac
    12061 aacgatcgac tgattggctc attaaagaaa atgcgtgatc ttggcaacac cctcgttgta
    12121 gtcgaacatg atgaagatac gatgtgggca gcagattatt tgattgacgt cggacctggg
    12181 gccggagaac aaggcggaca aatcgtggct gcaggcactc ctgaagaagt agctaatgat
    12241 gaaaattctt tgacagggaa atatctttca gggaaaaaat caatccctgt accaaaagaa
    12301 cgccgtaaag gtaatgggaa agcaatcaag atcactggtg cttcagaaaa caatctgaaa
    12361 aatattagtg ttgaatttcc attaggagaa tttgtagcag tcacaggtgt gtctggttct
    12421 ggaaagagta cattggtcaa tagtatctta aagaaatcgt tagcgcaaaa attaaataag
    12481 aattctgcta agccaggtaa attcaagaca atttccggct acgaaagtat cgaaaagatc
    12541 atcgatatcg atcaaagccc aatcggccgg acgccgagaa gtaatccagc gacttataca
    12601 agtgtatttg atgatatccg tgggttattt gctcaaacga acgaggcaaa aatgcggggt
    12661 tataagaaag ggcgttttag tttcaacgta aaaggcggtc gttgtgaagc ttgtcgcggg
    12721 gatggaatta ttaagatcga aatgcacttt ttgcctgatg tctatgttcc ttgtgaagta
    12781 tgtcatggta aacgatataa ttctgaaaca ttagaagtgc attacaaagg aaaaagcatt
    12841 gctgatattt tggaaatgac agtagaggat gctgtagaat tcttcaagca cattccaaag
    12901 attcatcgca aactgcaaac gattgttgat gttggcttag gttatgtgac gatggggcaa
    12961 ccagcaacga cattgtccgg tggtgaggca caacggatga aacttgccag tgaattgcac
    13021 aaaatctcta atggaaagaa tttctatata ctagatgaac caacgacagg acttcatagc
    13081 gatgacatcg cccgcttgtt gcatgtatta caaagattag tagatgctgg taacacagtt
    13141 ttagtgattg aacacaatct agatgtaatc aaaacagcag attatatcat tgatttagga
    13201 ccagaaggtg gagaaggtgg aggaacgatc cttgcgactg gaacaccaga agaaatcatt
    13261 aacgtaaaag aaagttatac aggtcactat ttgaaaaaaa taatggtata aagaggatag
    13321 atagctgaga tagttaaaaa aacataaaaa aatgaatttt tgcttgtttt attaatatca
    13381 gtgttctaga atcattacat ggtagttctt tctacactga ttggtataga taaaaacttc
    13441 ctataagttc aatccgttca aaaaagaatt gagcaatcgg aggactttcc tttgattgct
    13501 tttttcttta tggtatgata ttccttgtgt agttggaaaa gaaaaacaag agattatgag
    13561 taagtgagag ataatgatga acagtattga aaatgaagaa tttatcccta tattattagg
    13621 aagcgacatg aacgtttatg gtatggcacg ttcttttaat gaagcatacg gcaaaatctg
    13681 ccaagcatat gcttcggatc agttagcccc tacccgctac agtaaaattg tcaatgtaga
    13741 agttattcca ggatttgata aagaccctgt atttatcgag acgatgcttc gtttggcaaa
    13801 agaaagatat tctgataaga gtaaaaaata tcttttgatt gcttgtggtg atggatatgc
    13861 agaattgatc tcccaacata aacaagagc
//
LOCUS       DAFFSV010000073        13834 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFFSV010000073 DAFFSV010000000
VERSION     DAFFSV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.073"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            11..694
                     /locus_tag="LR110_001878"
     CDS             11..694
                     /locus_tag="LR110_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL4822834.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            691..1464
                     /locus_tag="LR110_001879"
     CDS             691..1464
                     /locus_tag="LR110_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL4822835.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1461..1859
                     /locus_tag="LR110_001880"
     CDS             1461..1859
                     /locus_tag="LR110_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBL4822836.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            2044..2715
                     /locus_tag="LR110_001881"
     CDS             2044..2715
                     /locus_tag="LR110_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL4822837.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2774..3214
                     /locus_tag="LR110_001882"
     CDS             2774..3214
                     /locus_tag="LR110_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822838.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3226..3894
                     /locus_tag="LR110_001883"
     CDS             3226..3894
                     /locus_tag="LR110_001883"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL4822839.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3958..4581
                     /locus_tag="LR110_001884"
     CDS             3958..4581
                     /locus_tag="LR110_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBL4822840.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4637..5023
                     /locus_tag="LR110_001885"
     CDS             4637..5023
                     /locus_tag="LR110_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBL4822841.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5236..6627
                     /locus_tag="LR110_001886"
     CDS             5236..6627
                     /locus_tag="LR110_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBL4822842.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            6974..7621
                     /locus_tag="LR110_001887"
     CDS             6974..7621
                     /locus_tag="LR110_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL4822843.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    7750..7966
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8014..10659
                     /locus_tag="LR110_001888"
     CDS             8014..10659
                     /locus_tag="LR110_001888"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBL4822844.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            10969..12282
                     /locus_tag="LR110_001889"
     CDS             10969..12282
                     /locus_tag="LR110_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBL4822845.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            12272..12925
                     /locus_tag="LR110_001890"
     CDS             12272..12925
                     /locus_tag="LR110_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL4822846.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            12980..13651
                     /gene="radC"
                     /locus_tag="LR110_001891"
     CDS             12980..13651
                     /gene="radC"
                     /locus_tag="LR110_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBL4822847.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
BASE COUNT         4466 a         2303 c         3010 g         4055 t
ORIGIN      
        1 aggaggatac atgctaaaaa attttaaaga agtgattgct gctatattgc cgatgactgt
       61 cctgattgtc attttaacgt ttgtttttgc accgttagaa ggagaagacc tcgcttcatt
      121 tctggttggt gcggcaatca tgatgatcgg aatgacgctc tttttgtttg gagcagatta
      181 ttctatgatg gaagtcggag atctagtagg gaaatatatg atcaaaaaga aaagtttgac
      241 cgttttggtc agtttgggat ttgctatcgg gatcgtgatc acgattgctg aaccttccgt
      301 gcaagttttg ggacaacaag tatatgatat atctgacgga gagattggca gagtgttgct
      361 gattgggatc gtcagcgtcg gcactggagt atttctagcc tttgcgctgc ttcgggtcgt
      421 attcaagcta tcctattatc aattgatggc tataggctac ataggcgttt tgatcgcctc
      481 atttttcacc agttccgaat ttatgccgat tgcttttgat tcggggggag tgactactgg
      541 tccagtaact gttccattta tcttggccct tgccggaggg atgactagta tgatcaaaca
      601 aaagaaaaat gaaaatgaca gttttggaat ggtcggtatt gcttcgttag ggccgattct
      661 tgccgtgatg attttggggg tgatcttcca atgagtttct ggcagcaagt gatagatgga
      721 atcgaaatcg ttaccatgga agtcattttg gcagtggcac ctatcgtaat cctatttgtt
      781 ttaatgaata tcattagttt tcgtatgcgc aaaaagcgtt tctttgacat tatgaaaggt
      841 ttcttgataa cgactattgg gttgatcctt ttcctgcacg gagtcaatat ggcttatgta
      901 ccggtaggac agcatttagg cagtgctatt gcgggattgc cttacaatta tatactggtt
      961 ccccttggct ttttaatggg gttcttagta gggtttgctg agccggcgat tcatgtaatg
     1021 atcaaacagg ttgaagaatt gagcgagggg agaatccgat cgaaaatcat gctgatggta
     1081 atttcaattg ggatcggcgc agcagtagga ttgtctatgt ggcgtttgct cgtcggattc
     1141 tctctttatt atttcttgat cccaggatac gggctagcat ttattttagg acgaaaagtg
     1201 gacaagatct ttttagcaat ggcttttgat aacggtggag tagcaacggg cccgatgtgt
     1261 tcgacattta ttttatcaat gagcgtggca atcgctgcac aaattgaagg aagagatccg
     1321 atcattgacg ggtttggtgt ggttgcaatg attgcattga caccaatatt atccacttta
     1381 gtactaggat atatttataa acgaaaagat gaaaaagaca gaatcgaaaa agaaagattg
     1441 caaaaattgg gggaaagcaa atgaaagatc ttgtagcatt gaatcttcac atgattgtga
     1501 cgatcgttga cggaggaatg ggcgaagatg ttatcgactt ttcaaaagaa gcaggtgcag
     1561 caggcggaac gatcttacat ggaagaggtt ctggtgtaca tgattcaggg aaatttttag
     1621 gtttggacat ttcgccagaa aaagatattg tgttgacatt agtacccgat agtttgacga
     1681 ataaagtaat ggatgcgatt ggtcaaggca tcaacatcga tattccaggt aatggtattt
     1741 gttttagtat tgatgtagac aaagtcattg gtatcactaa gattcatcaa tatcgagaaa
     1801 ttatcggaat gcaagaactt ttagatgaaa ataacgatag agaaaagtct gaatcataga
     1861 caagcgtttg aagaggaaat aaacacaaac aagaaggcgg atgagcgtta tttccgcttt
     1921 tttgtttgta tttttctcgt gaatacttat gaaatagtaa atttttatga aattctactc
     1981 taagcatcag actttctttt caaaatatgc tattttaaaa atgatgaaaa aagggggaga
     2041 aaaatgaatt ttttagttgt tttaaaagat actctgattg agcgatctac atttctatat
     2101 gttcaattgc aggaaaataa aacattgctg catttttcac cagagttcca tctagagggg
     2161 ctaacattag gagaaatcta tcaagcagaa gggctgtcgg ctgttttgca cttttttaag
     2221 gtagaattgg aattaccagt aaaagattat atcgagcttt ctcttgaatc ttgggatcga
     2281 gcagtggttg aactgtttcc agaaggttta atgatcgata caaacgatgg gaagatccat
     2341 ctgactgcag cagaactgca aaaacaaatg cgttatcaaa tagatgacga aaattctttt
     2401 tctattttta gacgtcagca aaagattcta aaaagctttt taaaagtgat cagtaaaaaa
     2461 cggaatctga tcaaaacgac acagctgttg aaaaaatatc caggtctgct tcacacgtct
     2521 attcaatttc ctcaattgat tacgatgatc catcgtttta tacgtatgca acagctacca
     2581 tctaagaagc tgtttcttcc attgacggac acatttcgag tagtcaacag agaagataaa
     2641 aagaaagtga ttgtgattga ctttaccaga aatcggaatg tccttcacca agttgcaatg
     2701 gaaaaggcca attaaggctt tttctttttg gatttttttg tcataataaa tattagatga
     2761 tggaggagat aatatgatgc aaacatggtt agaaaggcta atacgatttc agcagaacta
     2821 tcacaaactt atgcagggaa gatatgcgaa atttgatcag ttgaatcgcg gtctattgat
     2881 cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc tatagaatcg gacaattgtt
     2941 gttcatcgtc tttttcggat ggatgatatt tcgtttccta tcaaaaaaaa tctaccccag
     3001 actaaatgag aatcaacgtt atttgaaata tcttgataga gtcaaagaaa aatatcatca
     3061 gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt tatacttttt ttgagtgccc
     3121 gaattgtcat caaaagcagc gtgcacctaa aggaaaagga cgaatacgtg tgacatgcaa
     3181 aacatgtggt ataaaattcg aaacgaatgt ttaagggaga aaagaatgaa attagtatta
     3241 ttacgtcatg gcgaaagcga agcaaatttt gaaaattact ggacaggatg gctagatgtc
     3301 gccttaacag aaaaaggaaa agaacaagca gcagttgccg gtcaaaaaat gcttgaagcc
     3361 ggacttcaat tcgatgccgt ttatacctct gttttgaaac gtgcgattct taccggacaa
     3421 gtcgcactag aagaaatggg tcagttatgg cttcctataa taaaaacatg gcgtctaaat
     3481 gaacggcatt atggggatct tgtgggtaaa aacaaagata agatgaaaga aatatacgga
     3541 aaagatcaag taaaaacatg gcgcagagga tatacggaag ttccgccgtt aaccgcagaa
     3601 aatagatttg accgcagata tgatcacttg gatccgcatt tgatcccata cggagaaagt
     3661 ctggaaatga cagtgaatcg gatcattccc ttatggcaag atcatctagc accaaaacta
     3721 aaagatcggc aagatttact agttgtgggt catggtaata gcatccgagc actgacaaaa
     3781 tatttagaag atgttcctga agatcaaatg gatacgatcg atatcccaaa tgcacaacca
     3841 atccagtata cattagcaga tgatttgaca attctggaca aaaaaatact ttaaagaaca
     3901 cctttatcta aaagtgagag atagaaaaaa gaagcaaagg aatggaacaa aaggaacatg
     3961 aaaaattata tatgggactt tgatggtaca ttgtttgata cgtatcccgc aatggtagat
     4021 ggtgcatggc aagcactaaa agattttggt atcagtatgg acaaaaaaga aatctacttc
     4081 aaaatgaaaa agtattctac tagttatttg atcaatgaat caaacttgaa tgccagagag
     4141 tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg aagtttcccg tccatttcca
     4201 gaaacgaaac aagtactgga aatgctaaaa gacaacggcg gtcgtcattt catcctgacc
     4261 caccgtttga cagaatcaac atggggcctg ttaaaagaac atcgattggc acatctgatt
     4321 gaggaagtag tagggatcga tcaggacttt cctcgtaaac ctgatccagc atctttaaat
     4381 tatttgatcg atacttttca tttagaaaga acagacacga tgatgatcgg cgatcgtcgt
     4441 ttagatatcg aagcagggaa aaatgcagga gtcgctactt gtctttacga catcgatcat
     4501 tttctgggtg agatcccggc agattatgtg gtaggaaatc tgaatgaaat ccttaccttg
     4561 cagagcatca ttaaaaagta gtatcccctt tcaaaggaga tatgttataa ttttcataaa
     4621 ggagtgataa aattatatgg aaatcagaag acaaaaagaa gttgtagaag catttcatta
     4681 tgatatgaga ttgccgaacc aagaagtaga aacagatttg aaagtaggct tctcgccttt
     4741 aaaagaaacg atcgaaaact atccgaaaga acatagcatc gtaggagcac gcttagagtt
     4801 ccgtttagtg tttgatacat atgttttatc tggaagtgtg agtcagatca atcatatcct
     4861 caatcggaaa attgaaggtc aaaatgatgt cacacaagaa gaagtggacg agctagtagc
     4921 accgttattc gatatcgtac gccgaatgtc ttatgaggtg acagagatcg ccttagatca
     4981 gcctggggta aaattgaatt ttcaatcaga aaaacaagaa tagacagtaa aaacatacct
     5041 gcgatagatt aaagctatca caggtatgtt tttttattac taattgtaag caaaaagaat
     5101 gtcatttcga taaaaatcgt ctaaaatata aaagtcactg aaaaaaattt tttcaggagg
     5161 atggttgaag aatcattctc gggaaccttt acttccttcg atggacttgt cattaataat
     5221 atgaggagga gaaaaatgac aacactcatc acagttttag tggttctagt ttttgcggca
     5281 ttattatatg tgttctatcg tatgcaaaag cgccatacaa aattttcaac acgcgtcttt
     5341 acagcattag gagttggtat cgtctttggc ggtctgcttc aattgatctt tggtgtagac
     5401 gacaaagtcg ttactcaatc aatggaatgg atcgggattg taggtcaagg atatgtatca
     5461 ttcttgcaaa tgctagttat gcctttagtg tttatttcga tcattggtgc attcacaaaa
     5521 atgaaagttt ctgaaaagct agggaaaatt agtgcgactg tattgacttc tttacttgga
     5581 acaacagcta ttgcagcatt gatcggaatt ggttcaacaa tgctgttcgg actccaagga
     5641 gctaaattca cacaaggagc tgcagaaaca gcacgaatct ctgaactggc aacacgtcag
     5701 gaaacagttg aaaacttatc gattccacag cagattttat cctttatccc taaaaacgtt
     5761 tttgctgatt tagcaggtag tcgtgcaaca agtacgattg gagtcgttat tttcgcggca
     5821 tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc caaaagaagc agcattcttt
     5881 tcagatttag tcgacagtct gtataaaatc attatgcgga tcgtgacgct tgttttgcgt
     5941 ttgacacctt atggagtatt tgctttgatg acaaacgttt tggcaacaag tgattttgca
     6001 gctatcgtca atttaggtaa atttgtcgtt gcttcttacg tagcattgct tgctgttttg
     6061 attgtccact cactgatttt gattggtgca aaagtcaatc caatcaacta tttcaaaaaa
     6121 gcctttccgg tattgagctt tgcgtttact tcacgttcaa gtgctggagc tttgccattg
     6181 aatatcgaga ctcagacaaa agctttagga gtagatgatg caacagctaa tttctcagca
     6241 agttttggtt tgtctattgg acaaaacggc tgtgctggcg tttatccagc tatgttggca
     6301 acgatcgtcg caccagcagc tggagtgaat gtctttagtt tggaatttat tttgatgctt
     6361 gtggcaattg taacaatcag ttcttttggt gtagctggag tcggtggggg cgcaacattt
     6421 gcttcactga tcgttttagg ggcaatgaac ttgccagtag caatcgttgg gttagttatc
     6481 tctgttgaac cattaatcga tatggcacgt acagcagtaa acgtcagcgg tagcatggta
     6541 gctggtatcg tgacaagtgc acgcatcaaa gatttagatc gcgatgtcat caatgatgat
     6601 tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt gtgatagaag tggtcagctt
     6661 taagaaataa gaagaagttt cctaaaaata gtttctcaaa tttttgtgaa atttcggctt
     6721 atttccgaga agctacttct gaaacaccgt gaataagagg ttgtgaaaaa gctgccctgc
     6781 atcaagtaat aagaacagcc aaacaaaaat agttcctcat attttacgtt tggttgagct
     6841 tattaccgca gatgcaagct tttgaacacc gttttatttg tctcacaccc ttttccttcc
     6901 tacttgtaac cttgctcccg tgttagaaac atgttacact aaatatgtga aattattaac
     6961 aaggagcgtc gttatgcgag aaaaaacaga tacactgcca aaagcaactg cgaaaagatt
     7021 acctttatat ttacgctact tgaagatgct ggatgactca ggtataagta gaatcaaatc
     7081 aaatgagttc agcgaaatca cccaaattcc ttctgcaacg atccgcagag acttctcgca
     7141 attaggcgag ctaggcagaa gcggatatgg ctatgatgtc ccttttctta tcgatgtatt
     7201 caataatatc cttaatacta aagaagaaaa acgaatcgca ttagtaggat atggaaatct
     7261 tggcaaagca ttgaaacaca acaattttag aagaaatgaa aatctaaata tcgtgtgcgt
     7321 ctttgataat gatccagcac tgatcaatcg agtaattgat ggagaaatga tctatccgat
     7381 tgatcgtttc gcggagatag ccaaagcaaa aaatgtgaca gtagcaatct ctacggtacc
     7441 aagtaaatat tcacaatctg ctattgatga aattgtcaaa ggaaatgtaa ccgctatact
     7501 taattttgcg cctgacagag taactgtccc agcttatgtc aatgttcaat acattgattt
     7561 gacaaccgag ttgcagacgc ttatttactt tgatgagaat tactcagaag ttttttctta
     7621 ggaaaatttg agtaggctta ttgatcttgc gagaactcgt cgtaattctt tcaaaaattt
     7681 tttagtacta aatgaagtgg ttttttcacg accacttgtt gactaaaaaa aaaaaccatt
     7741 gtagaatgaa gcataacgct gaacaagagg agtaactggc acattcattc ttagagagag
     7801 aactatctgc tgaaaggttc tcaatgacaa ccagtgaagg tagcttggga gtttctgtct
     7861 tgaatggagt agagatagac gagtcgtgat cgttaactca cttgagaggc aataatgatt
     7921 gattattgca atttaggtgg taccgcgtaa atcatacgtc ctagaatacg aaagtatttt
     7981 aggacgtttt ttagtttaaa ggaggaaaac agcatgacgg aagaaaataa cctatcaaca
     8041 aaatataatc cgcaagaagt tgaagctgga cgttatcagc attggttaga acaagatttg
     8101 tttaaaccga gcggagataa aaaagcaaaa ccttattcaa tcgtaatccc gcccccaaat
     8161 gttactggaa aacttcattt agggcatgct tgggatacga ccttgcaaga tatgctgatt
     8221 cgtcaaaaga gaatgcaagg atatgatact ttatggctgc ctggtatgga ccatgcagga
     8281 atcgcgacac aagcaaaagt agaagcgaag cttagagagc aagggatttc tcgttatgat
     8341 ctaggtcgtg aaaagttcat cgatcaagtg tgggaatgga aagaagagta tgcgagccac
     8401 atccgcgaac agtgggctaa aatgggctta tctttagatt acagtaggga acgttttaca
     8461 ttagatgatg gattatctga agcagtacgt aaagtttttg ttaccttata cgaaaaaggt
     8521 ttgatttatc gtggtgaata tatcatcaac tgggatcctc aagcacgaac agctctatct
     8581 gatatcgaag ttattcataa agatatcgaa ggcgcctttt atcatatgag ctatgaacta
     8641 gcagatggat caggcgtagt agaaattgcg acaacccgtc cagaaacgat gttgggagat
     8701 acggcgattg ctgttcatcc agaagacgag cgttatcaag cgctcatcgg caaaaaagtg
     8761 attctacctt tagtcaataa acaaatccca atcattgcag atgaatatgt agatatggaa
     8821 ttcgggacag gtgtagtaaa aatcactcca gcacatgatc caaacgactt tgaggtaggt
     8881 aatcgtcatg atcttccacg cgtaaatgtt atggatgatg atggtacgat gaatgaacta
     8941 gcaggaaaat atgctggaat ggaacgtttt gctgctcgta aagcaatcgt gcaagatctg
     9001 aaagaaatcg gtcgattgat taaaatcgat acgatgatcc acagtgtggg tcattccgag
     9061 cggacaggtg tcgttgttga acctcggctg tctactcaat ggtttgtaaa aatggcgccg
     9121 ttagcgaaaa aagccattga taaccaagaa acagatcaag cagtggaatt cttccctcca
     9181 agattcaatc aaacgttttt acgttggatg gaaaatgtcc atgattgggt aatctcacga
     9241 caattatggt ggggacatca aattcctgca tggtatcaca aagaaacagg tgaaatgtat
     9301 gtagggatgg aagcaccagc ggatagtgaa aactgggtac aagatcctga tgttttagac
     9361 acttggttta gttcagcttt gtggcctttc tcaacaatgg gctggccaga tacggaaagc
     9421 gaagattata agcgatactt ccctactagc actttagtaa ccggttatga catcattttc
     9481 ttctgggtaa gcagaatgat tttccaaagc ttggaattta ctggcgaacg tccatttgag
     9541 aatgtcttga ttcatggatt gattcgtgac gaacaagggc ggaagatgag taaatcatta
     9601 ggtaatggaa tcgatccgat ggaagtaatc gaaaagtatg gagcagatgc tctccgctgg
     9661 ttcttatcga atggttctgc accaggacaa gatgtccgct tcagctatga aaaaatggat
     9721 gcttcttgga actttatcaa caaaatctgg aatgcttcac gtttcgttat catgaatatc
     9781 gaaggaatga ccgtagaaga gatcgattta tctggagaaa aatcagtggc tgaccggtgg
     9841 atccttactc gtttgaatga aacgatcgaa cgagtaactg agctatttga tcgttttgaa
     9901 tttggtgaag caggtcgaca actgtataac tttatctggg atgatttttg tgactggtac
     9961 atcgaaatga gtaaagaagt actttatgga gatgatgctg tttcaaaaca aaccacccgt
    10021 agcatcttag tccatacact tgatcagatc ctacgtttat tgcatccaat catgccattc
    10081 gttacagaag aaatatggga acatattcct catcagggaa attctctagt agtggcagaa
    10141 tatcctgttg tacgtccaga gtttgatgat gaaacagctt ctaaagggat ggaagtattg
    10201 aaagaattga ttcgagccgt tcgaaatatt cgttcagaag tgaatacacc attgtctaaa
    10261 ccaattacgt tgctaattaa aacaaacgat gctaaaatcg atcaattttt agttgaaaat
    10321 acaagctata tcgaacgctt ctgtaatccc gaagagttga cgatatcaag tgagatcact
    10381 gcgccagatc tagcgatgtc agctgttctg acaggtgcag aaatcttctt gccgttagca
    10441 ggattgatca atattgaaga agaaatcaaa cgattagaaa aagaactgga aaagtggaca
    10501 agcgaagtaa aacgtgtcca aggaaaactt tctaatgaac gttttgtttc taacgcgccg
    10561 gaagaagtag tagaggcaga gcgtgcaaaa gaaaaagatt atttagagaa gcaaacggct
    10621 gtcaaagaac gaatcgaaag tttgcgtact attcaataag aatagaaaaa gagagtgtga
    10681 aatcagctag ctattactaa aagctagctg attgaacatc gttacaggaa agagtttgag
    10741 cctcactctt agcttcgatt cataaggaga tgtgacataa attttgtcgc atctcttttt
    10801 tagtctactc ttattatgta ggagaaggag ggaggtggat catgtatcaa gagtcaataa
    10861 agtagttgat aaaatcgttc ctctctctgt ttcttttctc tttgtcatac ttttattttt
    10921 cttatgtgtt ctatttgtta taataaaaga gaaatggggg aattgttgat gacgattgaa
    10981 gaagcaatag aatggatcca tagtcgatta ccatttggtt cacgtcctgg ccttgaccga
    11041 gtcgaagctt tattagaagt ggtagggcat ccagagagaa aagtaaaaac gatccatatc
    11101 gctggaacaa acggaaaagg ttctactgta gcttatttga gaagcctatt agaagaggct
    11161 gggctgacag taggtacatt tacttcacct tatatcgaaa cgttcaatga acggatcgca
    11221 atcaatggca aaagcattcc agacaatgat ttgatccagc ttgtaaaaaa atatcagcca
    11281 ctgatcaaac aacttgatca atttgaagca gtagctggta ttactgaatt cgagacatta
    11341 acagcaatgg catttgatta ttttttgatg aagaaagtgg acgtagcgat tatcgaagtt
    11401 ggcttaggcg gcttattgga ttctaccaat gtagctgtac caatgctgac gggaataaca
    11461 acgattggac ttgatcatac cgaaatactt ggagatacaa ttgaagaaat cgctgcacaa
    11521 aaagctggaa tcatcaaaca aggcgttcca gtagtaacag gaaatattgc cccagaagct
    11581 tttactgtca tccaacaaac agctaaagca aaaaatgcac cgctttatgt atacggggaa
    11641 aattataaaa taaactatct gcatcctgat gagatgtggg gagaagtatt tgaatttttt
    11701 aatgaaatag gaaagatccc tcaattaacc actccgcttt tagggcaaca tcaagctgaa
    11761 aatgcaggag tagccatcga attgtatttt gtttactgtc aattggaaaa gctgcctttc
    11821 cgagaaaaag atgttttgaa gggattgaag aaatctcgat ggccaggaag aatggagcgt
    11881 ttgagtaacg agccattagt tgttttagat ggtgcccata atacgcatgc aatgaaccgg
    11941 cttgttgaga atttgaaaaa agaattcaaa ggatatcaaa tcaacatttt gttttccgcc
    12001 ttacgaacta aaaatgttag agaaatgcta gaacgattgc agcaagtacc aaatgtccat
    12061 atttatttga cgacctttga acatccaaaa gccatcgaat taagtgaagt atcagattta
    12121 gcaagtgaga gagtatcaat cgtttcattg tggcaattcg gattagcaga tatattggaa
    12181 aagatgacaa atgaagacgt attagttgtg accggttcac tttatttcat ttcagaagtt
    12241 cgccatttat taatacaatt aggaggagat catgaagtat aatgcagtga tttttgacat
    12301 ggatggtttg ctgttcgaca cagagttagt gtattacgaa gccacacaag tagtagcaga
    12361 caagatgggt tttccttatg ataaagacct gtatttaaaa ttcttaggtg tttcagatga
    12421 agaagtatgg gcgaattatc atatgatttt ctcggaattt ggcaaaacaa gtgtacaaag
    12481 attcatagat gattcatacc aggaaacgct tgaacgtttt tcgcaaggag cggtacaaat
    12541 gaagcccggt gtgttggaac tcctctcttt tttagaagaa catcagattc ccaaagttgt
    12601 cgcttccagt aaccaacgag cagtcattga acttttattg aagaagaaca atctgtttga
    12661 acgatttgag acgattgttt ctgcagaaaa tgttcaacga gcaaaaccag acccagaaat
    12721 tttcttgtta gctaatcaga agctaggtac agcgaagaaa gaaacattga ttttagaaga
    12781 ttcacaaaat ggtattttag cagcttctgc ggctgagatc ccggtagtca tggtaccaga
    12841 tctgcttcca ccatcccaag aattgatcaa aaaaacagct ggggttgttt cttctttaca
    12901 tgaaatccct gcattgttaa aataaaaatc catctctttt cgtatcttat tttagataag
    12961 atagaaagga gatgtttttt tgggaaaacg attgatcaaa gaagtaccta ctagctcttt
    13021 accaagggaa cgtatggaaa tttatggcgc ggaagcttta tcggatcagg agcttttggc
    13081 aatcttactg cgtaccggtc aacaccctta tagtgtgatg tcgattgctg gaaatctgtt
    13141 aaaaacgttt ggaggactgg cctctttacg acaggctact ttgcatgagt tagaagagat
    13201 tcgagggata ggacgcgtga aagcaatcga aatcaaagcc ttgatcgaat taggtcgacg
    13261 aattcagacg gatcatgaaa gagcggctcc accggttcgt accagctacg aattggccca
    13321 gcaattgatt ctggaaatga aagatcacaa acaagaacat ttggtttgta tttatttgga
    13381 cacgaagaat caagtgatct taaaaaaaac agtgttcatc ggttcgctga atcagtcagt
    13441 cgctcatcct agagaaattt tccattatgc tgtacgttat tgtgcagcaa ggatcgtttt
    13501 agcgcataat catccttccg gaatatataa gttagtacac ataaattata taaggaaatt
    13561 tactgttata ttgccatctg gaaatactat tatgttatcg ataattgaac gccataatgc
    13621 atttttttct tcttccgaag attctttata aatttcatcg aattcttttg atagaataaa
    13681 ttcgacattt tttatatcta ttttagctgg ctctacttca atttcattta attctttcat
    13741 taaagcttct cgatcttttt tgtattcctc taagtctata acttcattca agtaggcttt
    13801 cttcaatcta tctaatttat tctcaatatt tttc
//
LOCUS       DAFFSV010000074        13806 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFFSV010000074 DAFFSV010000000
VERSION     DAFFSV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.074"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..210)
                     /locus_tag="LR110_001892"
     CDS             complement(<1..210)
                     /locus_tag="LR110_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822848.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="LR110_001893"
     CDS             complement(216..446)
                     /locus_tag="LR110_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822849.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="LR110_001894"
     CDS             1063..1725
                     /locus_tag="LR110_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822850.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="LR110_001895"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="LR110_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL4822851.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="LR110_001896"
     CDS             5145..5459
                     /locus_tag="LR110_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL4822852.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="LR110_001897"
     CDS             5472..5846
                     /locus_tag="LR110_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL4822853.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="LR110_001898"
     CDS             5856..6200
                     /locus_tag="LR110_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBL4822854.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="LR110_001899"
     CDS             6271..7623
                     /locus_tag="LR110_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL4822855.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="LR110_001900"
     CDS             7683..8825
                     /locus_tag="LR110_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBL4822856.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="LR110_001901"
     CDS             8850..9104
                     /locus_tag="LR110_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822857.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /locus_tag="LR110_001902"
     CDS             9360..10544
                     /locus_tag="LR110_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL4822858.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="LR110_001903"
     CDS             10541..10678
                     /locus_tag="LR110_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL4822859.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="LR110_001904"
     CDS             10683..10904
                     /locus_tag="LR110_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822860.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="LR110_001905"
     CDS             10917..11417
                     /locus_tag="LR110_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL4822861.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="LR110_001906"
     CDS             11514..13361
                     /locus_tag="LR110_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBL4822862.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..>13806
                     /locus_tag="LR110_001907"
     CDS             13364..>13806
                     /locus_tag="LR110_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HBL4822863.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDA"
BASE COUNT         4666 a         2209 c         3023 g         3908 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcaca
//
LOCUS       DAFFSV010000075        13645 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFFSV010000075 DAFFSV010000000
VERSION     DAFFSV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.075"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            21..263
                     /gene="yaaA"
                     /locus_tag="LR110_001908"
     CDS             21..263
                     /gene="yaaA"
                     /locus_tag="LR110_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBL4822864.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            250..1374
                     /gene="recF"
                     /locus_tag="LR110_001909"
     CDS             250..1374
                     /gene="recF"
                     /locus_tag="LR110_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBL4822865.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1371..3317
                     /gene="gyrB"
                     /locus_tag="LR110_001910"
     CDS             1371..3317
                     /gene="gyrB"
                     /locus_tag="LR110_001910"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBL4822866.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3337..5808
                     /gene="gyrA"
                     /locus_tag="LR110_001911"
     CDS             3337..5808
                     /gene="gyrA"
                     /locus_tag="LR110_001911"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBL4822867.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6044..6343
                     /locus_tag="LR110_001912"
     CDS             6044..6343
                     /locus_tag="LR110_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBL4822868.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6393..6905
                     /gene="ssb"
                     /locus_tag="LR110_001913"
     CDS             6393..6905
                     /gene="ssb"
                     /locus_tag="LR110_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL4822869.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6931..7167
                     /locus_tag="LR110_001914"
     CDS             6931..7167
                     /locus_tag="LR110_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBL4822870.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7310..9283
                     /locus_tag="LR110_001915"
     CDS             7310..9283
                     /locus_tag="LR110_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBL4822871.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9289..9741
                     /gene="rplI"
                     /locus_tag="LR110_001916"
     CDS             9289..9741
                     /gene="rplI"
                     /locus_tag="LR110_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBL4822872.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10015..11382
                     /gene="dnaB"
                     /locus_tag="LR110_001917"
     CDS             10015..11382
                     /gene="dnaB"
                     /locus_tag="LR110_001917"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBL4822873.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12162..13454
                     /locus_tag="LR110_001918"
     CDS             12162..13454
                     /locus_tag="LR110_001918"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBL4822874.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4543 a         2475 c         3008 g         3619 t
ORIGIN      
        1 atgaaaatga gggatgggca ttgaaacaac aagtcattct aaatacggaa ttcatgacgt
       61 tgggccaaat gttgaaagaa gtcacagtta tcggtagtgg cggacaagca aaatggtatt
      121 tggcagaaaa cactgtgtta gtcgatggcg aaccagaaaa tcgtcgtgga cgcaaacttt
      181 atcccggcat gatggttgaa gttccagaag taggaacttt ttttatggta aaaaaaggaa
      241 acgacgataa tgaggctgaa taagctgtat ttaaaaaatt atcgaaatta cgaagagctg
      301 aacatcgaat tttccaagaa cctcgtcatc tttttaggag aaaatgcgca aggaaaaacg
      361 aatcttttgg aaagtatcta cgtattagct atgacgcgaa gtcatcggac aagtagtgag
      421 caggaattga ttggctggac tgacgatcag gcgatgattc aaggagagat cacaaaaggt
      481 tcatccactc ttccattaga aatcttatta tctaaaaaag gccgaaaaac caagatcaac
      541 catatcgaac agaaaaagtt aagtacttac gtgggacaaa tgaatgtcat tttatttgca
      601 ccagaagatc tttctttagt aaaaggcagc ccgcagatca ggcggaagtt tttagatatg
      661 gaattaggcc agatcaatcc aatctatctt tatgatcttg tccaatacca agcaattttg
      721 aaacaaagaa accagtactt gaagcaattg aatgaaaaaa aacaaactga tttgctttat
      781 ttggatattt tgtccgaaca actagcagcg tttggcagca aagtattgaa agcaagagca
      841 cagttcgtca agcgattgga gttttgggct aattctttgc atcagcaaat cacccatcaa
      901 aaagagcagc tggaaatcga gtatctcacc gcagtcgatt ctttagaaac acatacgcaa
      961 gagcaaatcc aagaacaatt tctcgcactt ttgaaccaaa ataaaaagaa agaccttttt
     1021 cggggaacaa cgacagttgg tcctcatcgt gacgatcttt ccttttttat taatcagaaa
     1081 aatgtgcaga cttatggatc gcaaggacag caaagaacaa ctgcactaag tgtcaaatta
     1141 gcagaaatcg atttgattaa agaagagaca ggggaatatc caatcctttt gttagatgat
     1201 gtgatgagcg agctggatga taatcgtcaa ctccatctgc tagaaacaat cgaaggaaaa
     1261 gttcaaacgt tcttaacaac aacgacactt gaccatgtaa aaaataaaat gactgttgaa
     1321 cctgagattt tttatgttca acaagggaaa ctaaaaggag aagaaactga atgacagaag
     1381 aaagaagttt agtagaacgc gctaaagagt atgatgccag tcagattcaa gtacttgaag
     1441 gacttgaagc cgttcgtaaa cgacctggca tgtatatcgg ttcgactagt tctgagggtc
     1501 tgcatcacct tgtttgggaa attgtcgata attcgatcga tgaagtatta gcaggttttg
     1561 caacgaaaat ccatgtgatt attgaaaaag acaacagtat caccgttatt gacgacggtc
     1621 gggggatacc tgtagatatc caggcaaaaa ctggacgtcc tgcagttgaa acggtcttta
     1681 ctgttttaca tgctggagga aaattcggcg gtggtgggta caaagtttct ggtggacttc
     1741 acggagtagg atcttctgtt gtaaacgcgc tttctacaca gcttgatgtc aaagtataca
     1801 aagaaggaaa tgtgtactat caagaatatc gtcgtggagc tgtagtggac gacttaaaga
     1861 ttatcgaaca aacggatcgc catggaacaa cggttcactt tacgccagac cctgaaattt
     1921 ttacagagac aactgagttt gatttttcaa aattggctac ccggatccgt gaacttgctt
     1981 tcttgaaccg cgggatgcgt atatcgattg aagacaaacg agaagaagaa ccagtcatca
     2041 atgaatacca ttatgacggc gggatcaaaa gttatgtcga atacttgaat gcaaacaaaa
     2101 ccgtgatttt tccagaacct gtatatttgg aaggtgaaca gcaagatatc gcagttgaag
     2161 tctctatgca atatacagac ggctaccatt ccaatatcat gagttttgcc aacaatatcc
     2221 atacgtatga aggtggaaca catgaatcag gattcaagac tgctttaact cgtgtgataa
     2281 atgattatgc ccgaaaacaa aagctgatga aagaaaatga cgacaacttg accggtgaag
     2341 acgtgcgaga aggattgaca gctgttattt ctatcaagca tcctgaccct caattcgaag
     2401 gacaaacaaa aacaaaattg ggcaattccg aagtacggac agtcactgat cggttattta
     2461 gcgaacattt catgaaattc ttgctggaaa acccaagtgt gggccggcaa attgttgaaa
     2521 aaggattact agcttccaga gctcgtctag cagcgaaacg tgcgcgtgaa gtaacgagac
     2581 gtaaaggtgc tttagagatt agtaatctgc caggaaaatt agcggactgc tctagcaacg
     2641 atcctgaaaa atgcgaattg ttcatcgtcg aaggagattc ggccggcgga tcagctaagc
     2701 aaggtcgtaa tcgagagttt caagccatct tgcctattcg tgggaaaatc ttgaatgttg
     2761 aaaaagcaag catggacaag atcttggcaa atgaagagat ccggtcttta tttactgcta
     2821 tgggtacagg gtttggagaa gattttgatg tatcgaaagc tcgctaccac aaattagtta
     2881 tcatgactga tgccgatgtc gacggcgcgc atatccgtac attgctgctg actctttttt
     2941 atcgttacat gcgtcctatt gttgaagctg gatacgtcta tattgctcag ccacctttat
     3001 acggggtaaa acaagggaag aatattacgt atgtacaacc aggaaaaaat gcagaagaag
     3061 agttgaaaca agtcgttgct tcacttcctg cctcaccaaa accaagcgtt cagcgttata
     3121 aaggacttgg agaaatggat gatcatcaac tatgggaaac aactatggac ccaagcaatc
     3181 ggatgatggc ccgcgtcagt gtggacgatg ctattgcagc agatcagata tttgaaatgt
     3241 tgatggggga tcgagtagaa ccccgtcgtg cattcatcga ggaaaatgca cactatgtga
     3301 agaacttaga tatttaatca tgaaggagga aaaagaatga gtgaagaaat cagagaaaac
     3361 atccatgatg tcaatctaac cagcgaaatg aaagactcct tcatcgatta tgcgatgagt
     3421 gttattgtcg cacgtgcact tcctgatgtc agagatggtc tgaaacccgt tcatcgccgt
     3481 attttatacg gcatgaacga attaggtgta acaccggata aagcccataa aaaatctgcc
     3541 agaatcgtcg gagatgtcat gggtaaatac catcctcatg gagacagagc gatctatgag
     3601 tcaatggtac ggatggcaca gccattcagc taccgttata tgctagtgga tggtcatgga
     3661 aactttggtt ctgttgacgg tgacggtgcc gccgctatgc gttataccga agcacggatg
     3721 agcaaaatcg ctacagaaat gctgcgtgat atcaataaga acactgttga tttccaaagc
     3781 aactatgatg atacagaaaa agaaccagtt gtactacctg ctcgcttccc gaatctttta
     3841 gtgaacggaa caacggggat cgccgtaggt atggccacga atatcccacc tcataacttg
     3901 tctgaggtag tagcggctat cgatttgctg atggataatc ctgatgtgac gaccaatgaa
     3961 ttgatggaag tattacctgg accagatttt ccaacaggtg gtttggttat ggggaaatca
     4021 ggaatccgaa gagcgtatga aacaggtaag ggttctatta ccgtacgtgc aaaagttgag
     4081 attactgaga tgccaaatgg taaagaacgg atcatcgtga cagaattgcc ttatatggtc
     4141 aataaagcaa aattgatcga acggatttct gaactgcaca gagacaagcg tatcgaaggg
     4201 atcacagatt tgcgggacga atcttcccgt gaaggtatgc ggatcgtcat tgacatccgt
     4261 cgtgacgcca gcgcatccgt tatcttgaat aacttatata aattgacatc cttgcaaaat
     4321 tcatttggat tcaatatgtt ggcgatcgaa aaaggtgtgc cgaaagtttt gagcttgaaa
     4381 caaatccttg aaaactatat cgaacatcaa cgcgaagtca tcacacgacg gacggtcttt
     4441 gaaaaagaaa aagcagaagc aagagcacac atcttagaag gtttgcgtat tgctttggat
     4501 catatcgatg aaatcattgc gattatccgc gggtctaaat cagatgacga ggcaaaagca
     4561 acaatgatcg aacggttcga tttatccgat cgccagtctc aagctatttt agatatgcgt
     4621 ttacgccgat tgaccggttt ggaacgggaa aaaatcgaaa atgaatacca agaactctta
     4681 aaactgattg aagatttaac tgacattctg gcaagaccag aacgtgttac agaaatcatc
     4741 aagaatgaat tagcagactt gaatcaacga tttggtgata aacgacgcac agaattacta
     4801 gtcggtgagg tcttaagtct ggaagatgaa gatttaatcg aagaagaaga aatcgttatt
     4861 acattgacaa acaatggtta catcaaacgt ttggcaaata atgaattcag agctcaacga
     4921 aggggtggac gtggtgtcca aggaatgggt atccacaatg acgacttcgt caaaaatctg
     4981 gtttcttgtt ctacccatga tacattgtta ttctttacga ataacggaaa agtgtatcgg
     5041 gcaaaaggat acgaaattcc agaatacggg cgtacagcca aaggaatccc aatcatcaat
     5101 ttactaggta tcgattcgag tgaaagcatc caggcaatca ttgcggtgac gggagaagca
     5161 gaagaaggcc actatctctt ctttacgacg cgtcaaggta cagtaaaacg tacttctgtc
     5221 actgcttttg ctaacatccg aagtaatggt ctgatcgcaa ttggtttgaa agaagacgat
     5281 gagctcgtca atgtgctact gacagatggt caatctaata tcattattgg aacacataac
     5341 ggctactctg tcactttcaa agaagaagca gtccgcgata tgggacgtac agcttccggg
     5401 gtcagaggga tacgcctgcg tgaggaagat tatgtcgttg gggcagcctt aatgaaggaa
     5461 aatcaagaag tattagtgat cacagaaaaa ggttacggaa aacggacaaa agtatcggaa
     5521 tatccagtga aaggccgcgg aggtaaaggg atcaaaacgg cgaacatcac tgaaaaaaat
     5581 ggtccactag ctggattgac cactgtttct ggagatgaag acatcatgct gattacagat
     5641 aaaggtgtca ttatccgttt caacgtatcg actgtttctc aaactggacg ttcaacatta
     5701 ggcgtacgtt tgatgaagat ggaagctgat acgaaagttg tcacgatggc agcagtcgaa
     5761 ccagaagctg cagaacaaac agttgaagaa caaggatcag aagaataacc atcaagacta
     5821 agtgtgtaag ctagcaagaa taaccgatta aaaaatctgg ttattcctgc tggctttttt
     5881 gattagttga atttctctta aaatattcga aactgttcat tctgtattgc ttttgaccaa
     5941 aatagagtgt ataattagag cttgtgagta gtctaattaa ttagataact ctccttgctc
     6001 ttaggaacct aagagccgaa agtccacagg gaggtgaata tcaatggaaa acacgaaata
     6061 tgaaatcatg tacattattc gtccaaacat tgatgaagaa gcaaaaactg ctttaatcga
     6121 acgtttcgat gcaatcttga aagataacgg agcagaagtt atcgaatcaa aagattggga
     6181 aaaacgccgt ttcgcttatg aaatgaacgg ttaccgtgaa ggtatctatc acatcgttaa
     6241 agtatcttct ccatcatcag caaatgctgt caatgaattt gaccgtcttg ctaaaatcaa
     6301 cgacgatatc attcgtcaca tgattgttaa agaagaagct taattgttgt ttcatgtgaa
     6361 acaatagaaa acgatgagag gagatgacta ttttgattaa taatgttgta ttagttggac
     6421 gtctgacgaa agatccagat ttgcgttaca cagcaagtgg tacggcagtc gcaacattta
     6481 ctttggctgt gaatcgtaat tttacaaacc aaagcggcaa tcgcgaggct gattttatca
     6541 actgtgtgat ttggcgcaaa tcagcagaaa ctttagcaaa ctatgctcgt aaaggaacac
     6601 ttttaggtgt tactggacgt atccaaactc gttcttatga taatcagcaa ggacaacgtg
     6661 tatacgtgac agaagtagta gctgacaact tccaattgct ggaaagtcgt tctgcatctg
     6721 aaaatcgcca acaaagcggc ggattccaaa gttctggtca atcagctggc ggatttggcg
     6781 gaaacaataa ctctaatcaa acatctcaat catccaacgg tatgcctgat ttcgaccgtg
     6841 atacttctga cccattcggc tcaagttcaa cgatcgacat ttcagacgat gatttaccat
     6901 tctaaacgaa tttgatagga gggaaataga atggcacaac aaagaagagg cggacgtaaa
     6961 cgtcgtaaag tagactacat tgctgctaac catattgaat atatcgatta taaagatatc
     7021 gaattactaa aaagatttat ttcagaacgc ggtaaaatct tgccacgtcg tgtaactggt
     7081 acaggcgcta aaaaccaacg taaaattact gttgcaatca aacgcgctcg tatcatgggc
     7141 ttgctacctt tcgttagcga agactaataa atcctaggaa ttgaatgact gatccttttg
     7201 atcagctatt caattccttt ttttctgtaa aaataacctc tattcgttta gtcgctcgaa
     7261 tgacgaaagt tatggtaaaa tcaaattagt catatatggg aggaaaatta tggaaaataa
     7321 aaacaatcat ttatccccga aacctctctt ccttatcctg tttatattaa tcgaagtatt
     7381 ggtagtctta attttaccat ttcgttttgt tagcgcttcg gtcattctat tgatgaattt
     7441 atggggtgtt cgctattggt tgaaaatgaa acgttggctt attttaaata aacgtgaaaa
     7501 aatccagcag gcttcgactg tagctgaaac caatctagct tatgtctcag aagtcatgcc
     7561 tgtaggagtg attgcttatc agccatcaag ccaaaaaatc gaatggctca atccattagc
     7621 attgaaaatc aaagatcaaa gtggattaac agaggaagaa ttgctagata ctttttttac
     7681 tgcaaaagaa aaaaagaaaa accaagtcta tctagcagat aaaacatttc agtttgatat
     7741 ggatacagta aagggtgttg tttattttaa tgatattacc caaaaccagt tgttaaaact
     7801 ccatcagacg aacagtcagc cggtgattgg tattttatct attgataact atgcagatgc
     7861 cattgataaa ttagatgaca aagagatttc ttatttgaac tctttcgtca ctacactagt
     7921 ttctgactgg atgaatgaat accatgtctt tttcaaaaga atcaacgcag aacggttttt
     7981 cttcgtagca cagatgatcg atctgaaaaa gatgatggat aaaaaatttg atttgctgga
     8041 tcgctttcga gcagaagctg aaaaacagag ctcgcctatc acccttagta tggggattgc
     8101 ttatggctca agaaccctcg atgaaatcgg tggagaagct cagaataatt tagatgttgc
     8161 tttagtccgt ggaggcgacc aagttgtcgt gaaagaggta aaagaaggag caaaacctgt
     8221 ttattatggg gggaaatctg ctaagaatgt caaacgcacg cgtgttcgtt ctcgagcgat
     8281 gagtaccgct ttgcgtcgga tttttgatga aacaggcgat gtgtttatca tggggcacaa
     8341 gtttccggat atggatgctt taggctctgc ttttggtgtc agttgtctgg cacgcttcaa
     8401 ccagaaaaaa gcttacatcg ttatcaatga gaacgagatc attccagatg tagaacggtg
     8461 tcttgaagaa atccataagc atcctgaatt agaaatgcaa ctaatctctc cagaacgtgc
     8521 catagagctg aaaaaagaag aggacctcct cgtgatggtg gattaccata aaccttctct
     8581 atcgatctct cagggcctat atgagcaatt tgataagatt gtgatcattg atcaccatcg
     8641 tcgtggggaa gagtttccaa gtaaacctct gctgacttat attgaatctt cagcttcttc
     8701 agcttcagag ctagtttctg agctgatcca ataccaaagc agcaaaacaa atcgcttaga
     8761 tcgttttgag gccacattat tgcttgcagg gatggttgta gatacaaata gtttcaatgt
     8821 ccgtacaact gcacgaactt ttgacgttgc cagctattta aggtcttgtg gagcagacgc
     8881 atccttagta caatatttat tgagctcgga tttagcgaca tacttggaga tcagcaattt
     8941 gattgctgga agcgactatg tgacttctga tattgttgtg gcggcaggga cagacgaaaa
     9001 agagtataat agtgttatag cagcaaaaac agcagataca ttgctttcga tgatcaatat
     9061 caatgcagct ttcgtcatta caaagcggac agacggcttg attgggatca gtgccagaag
     9121 taccggcacg atcaacgtcc aaatcatcat ggaatcctta ggtggcggag gacacttcac
     9181 aaacgcagct actcaactgg aaaatacgac agttgaggaa acaaaacaga agctatttgc
     9241 tgcaatcaaa gaaaatatga atcaaatata tgataaggag taatccaaat gaaagtaatt
     9301 tttttacaag atgtaaaagg aaaaggtaaa aaaggggaca tcaaagaagt tccagcaggt
     9361 tatgcacaaa attatctaat caaaaataac ttggcaaaag aagcaacgaa aagcagtatc
     9421 agtcagttag caggacaaaa gaaagcacaa gaaaaacacg aagctgaaat cttggaagaa
     9481 gctaaaaagt taaaagaatt cttggaaaaa gaagatactg tagtcgaatt gaaggcgaaa
     9541 gcaggggaag acagccgttt gtttggttcg atcccttcaa aacaaattgc tgaagggtta
     9601 aacaaacaat acaaaatcaa attagataaa cgaaaaatcg aattagaaaa tccgatccgt
     9661 tcactaggct acacaaacgt acctgttaag cttcatcatg aggtgacggc tacgatcaaa
     9721 gttcatgtcg tacaagaata acaaatcttg atagcgatgt tcagctggta agtataacca
     9781 gaaggaaaaa agacaaaccg aaatttatct gagaaaacag ctgcagcaat gaaagcaaaa
     9841 gctgcaaatc gatttcagat tgatttcggt ttcttttttg ggaacagcaa aatagcataa
     9901 agaaaacgag tataataaaa gagaactatt tttttattac actttcggta cgaatgatga
     9961 cttttctctc attttcttgt aaaatgaagc aattgaagac aaggagcgat acgtatgaat
    10021 gaagtgtggc aggatcgaat cccaccgcaa aatattgaag ctgaacaagc ggttttaggc
    10081 tctgtttttc ttgacgcaga aacgatcatt gatgccatgg agtacataga gccaaaagac
    10141 ttttatcgtc gtggccatca aattattttt gagacaatga tcgagttaaa tgatcgtaat
    10201 gaagcgattg acgtggttac tgtcaaagac cggctggaac aagccaattt gttagaagat
    10261 gccggtggtt taagttatct ctcagacttg gcattagcag ttccgacagc agcaaatatc
    10321 gtctactatg cgaaaatcgt tgaagaaaaa tcattgctgc gtacattgat ccagacagca
    10381 acaggaatcg ttacgaaagg attcgagcaa ggagaagaag ttcaaacaat tttagatgat
    10441 gccgaacgca gtatcttgga ggtttcggaa aaaagaaatc gaagcggctt tttatctatt
    10501 gctgaagtac tgaactccac gattgaaaat atcgatcgac tagctcaaaa caatgaagaa
    10561 atcacaggtc taccaactgg ctatcaaggt ctggataaaa tgacagcagg tttccagccc
    10621 gaagaactaa tcattcttgc tgcacgtcca gcggtaggta aaacagcgtt tgcattgaat
    10681 attgctcaaa atgtaggaac aaaaacagat cgttctgttg cgatatttag tttggaaatg
    10741 ggggcagaat ctcttgtaaa tcggatgctt tgtgcagaag gttcgattga ggccagccat
    10801 ttacgtacag gtcagctttc ggaagaagaa tggtcgaatc tgatcgttgc gatggggagt
    10861 ctttctcgag caaatatttt tatcgatgat acaccaggga tcaaaatctc tgaaatccgt
    10921 gcgaaatgtc ggaaactagc gcaagaaaaa ggcaatctag gcttgatatt gatcgattat
    10981 ttgcagttga ttgaaggtaa cggtaaagag aaccgtcaac aagaagtatc agatatctct
    11041 cgccaactga aaaaactggc gaaagagctg aaagtacctg tcattgctct ttcacagtta
    11101 tctcgtggag tagaacagcg acaagacaaa cgaccagtgc taagtgatat tcgtgaatcc
    11161 ggatcaattg aacaagatgc ggacatcgta gccttcttgt atcgagatga ttattatgaa
    11221 agagaaggcg gagatgacga tgatggaaac gagccaaaaa atgacaacgt catcgaagtc
    11281 atcatcgaga agaaccgaag cggtgcacga ggaacagttg aactactgtt tatcaaagaa
    11341 tacaataagt tttcgtcgct ttcgccaaga gaagaatttt aaagaagagg ttgtgagcct
    11401 tgagcaatcc cgagagctct cgtaagaaga tgcaaggagt aaccgcagga acgactagag
    11461 tacttaattt aaacagatct ccaaagggat tgtgacaaaa gtcttgtcac agtccctttt
    11521 tccgaataaa cggtgttcaa tcagctgacc cttggtatta gctcagcaaa cgtcaaaata
    11581 tgaggaacta ttttgacgtt tgtttctcta atactcaggt caaaacgctg atcttacgac
    11641 ctcttttcct taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatcatca
    11701 aacatgagaa gcagtttttt gaaattgctc cttgtgactc agagttaaac ggcaggctca
    11761 caacctcttt cttaaacggt gttcaaaagc ttgcatctgc ggtaatacgc ccaatcaaac
    11821 gaaaaatatg agaagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
    11881 ttcacaacct ctgattcacg gtgttccaaa agtagcttct tcgaaaaata agtcgaaatt
    11941 tcacaaaaat ttgagaagca attttcgtac attccttctt atttcttgaa gctgaccact
    12001 tctgttacaa cctctttttt aataaccatt ctaaaaagat aaagttcgtt ttttaagcgt
    12061 ttttataaaa taaaaataaa aatcattcga ttttatttga aatatgtatc tattattgat
    12121 acaatggttc aggattaaat aatatagaac gaggtgttca catgtcatct gtagtagtag
    12181 taggtacgca atggggcgat gaagggaaag gaaaaatcac agactttctc agtgaaaatg
    12241 ctgaagtgat tgcacggtac caaggtggcg acaatgcagg gcacacaatt aagtttaacg
    12301 gtgtgactta taaactgcat ttgattcctt ctgggatttt ttataaagac aagatcagtg
    12361 tgatcggaaa cggcgttgta gtcaatccta aatcattggt taaagaatta gcttatttac
    12421 atgaaaaagg tgtagaaaca agtaatttgc gtatttctga ccgcgcgcac gtaatcttgc
    12481 cttatcatat caaattggac caattacaag aagatgctaa gggcgaaaat aagattggta
    12541 caacgattaa aggaatcgga cctgcctata tggataaagc agctcgtgta ggtatccgta
    12601 ttgcagactt attagataaa gaaatttttg aagaacgtct tcgtttgaat ctagatgaca
    12661 aaaaccgtca atttgtaaaa atgtttgatt cagaaccact tgattttgac gatatttttg
    12721 aagaatatta tgaatacgga caacaaatca aacaatacgt cacagatact tccgttatct
    12781 tgaatgatgc tttggatgca ggcaaacgcg tgttgtttga aggcgcacaa ggtgtaatgc
    12841 tggatatcga ccagggaact tatccttttg ttacttcttc taacccagta gccggtgggg
    12901 taacaatcgg aagcggtgta gggccttcaa aaatcaataa agttgtcggc gtctgcaaag
    12961 cctatacgtc acgtgtcggt gatggaccat ttccaactga attatttgat gaaacaggag
    13021 aacaaatccg cgaagtagga cgagaatacg gtacgacaac tggtcgtccg cgtcgtgtag
    13081 gctggtttga ttctgtagtt atgcgccatt ctaaacgagt ttcaggtatt accaacctat
    13141 ctttaaactc gatcgatgta ttaagtggat tggaaacagt aaaaatttgt acggcttatg
    13201 aattagatgg cgaactaatc taccactatc cagcaagctt gaaagagttg aaccgctgca
    13261 aaccaatcta tgaagaattg ccaggctggt cagaagacat tacaggatgc aaaacacttg
    13321 cagaactgcc agaaaacgca cgtaactatg tacgtcgtat ctcagagctt gttggtgtac
    13381 gtatctcaac attttctgtc ggacctgata gaacacaaac aaacatctta gaaagcgtct
    13441 gggcgcaaat ctaaggataa aaacgacgac tgaatcagga cgttcttacc agagcgagtc
    13501 gaagtgttta tctatacgat aataataact tgaagaggta gtgaaagaga cgtttagcct
    13561 tattgccgat aggctgtctc ttgaacactg tttattcaga ataagggact gtgacaaaaa
    13621 ccgtgtcaca gtcccttttt ccgaa
//
LOCUS       DAFFSV010000076        13576 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFFSV010000076 DAFFSV010000000
VERSION     DAFFSV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.076"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            214..1332
                     /locus_tag="LR110_001919"
     CDS             214..1332
                     /locus_tag="LR110_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822875.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1633..1953
                     /locus_tag="LR110_001920"
     CDS             1633..1953
                     /locus_tag="LR110_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822876.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            2405..2605
                     /locus_tag="LR110_001921"
     CDS             2405..2605
                     /locus_tag="LR110_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL4822877.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            2752..5541
                     /locus_tag="LR110_001922"
     CDS             2752..5541
                     /locus_tag="LR110_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL4822878.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDSSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAIYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKEIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            5588..6115
                     /locus_tag="LR110_001923"
     CDS             5588..6115
                     /locus_tag="LR110_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBL4822879.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            6136..7326
                     /locus_tag="LR110_001924"
     CDS             6136..7326
                     /locus_tag="LR110_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL4822880.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            7366..8664
                     /gene="asnS"
                     /locus_tag="LR110_001925"
     CDS             7366..8664
                     /gene="asnS"
                     /locus_tag="LR110_001925"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBL4822881.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            9459..10133
                     /locus_tag="LR110_001926"
     CDS             9459..10133
                     /locus_tag="LR110_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL4822882.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            10130..10471
                     /locus_tag="LR110_001927"
     CDS             10130..10471
                     /locus_tag="LR110_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822883.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            10501..10860
                     /locus_tag="LR110_001928"
     CDS             10501..10860
                     /locus_tag="LR110_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBL4822884.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            11384..13468
                     /gene="cadA"
                     /locus_tag="LR110_001929"
     CDS             11384..13468
                     /gene="cadA"
                     /locus_tag="LR110_001929"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL4822885.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         4534 a         2345 c         2925 g         3772 t
ORIGIN      
        1 ctttttctta gtttatcagc atttcatgaa gaatagttga agactggggc taaataattg
       61 aggaaattat gaagataact taaaaaaata gctaaattta aaaccgtaga aaccgtttta
      121 tttttttaga tacgttttat tgtatggaaa aatagtgttg tggtagactt tagatagaaa
      181 caaaggaatg atttttttag gaggggtacg tttatggcaa aaaaggataa agaattattg
      241 acacaattga cttcagaagg cgctaaagga ccattcgtca cgatcatgct gaatacgcat
      301 gtcgcccatc aagatgtgga aaaagatcag atcaaattca aaaattttgc gaaagaagca
      361 aagaaacgtt ttgagaaaaa atattccgaa catgactggt ctccgttcca agaaaaaatt
      421 gaaaatttgt taggagatcc ttcgttttgg cgtagtgcaa ctacgagtgt agcggtcatt
      481 ataactgctg accagaccta tatccatcgt ttgagtatcc cggtagacga tcaatactat
      541 gtaagtgatc ttccttattt actggctatt atcaaaaatg ggcagttcaa ctattcttat
      601 tatttattgg gattgaaccg cgactccatg aaattgtatg aggtcagcaa caaacaagta
      661 aaagaagtat ctctaccaga aggggcaccg gttgatgtcg ttactgcatt gggatctgaa
      721 ctgacaggtg gcggtttgaa cttctcttct aatggaggag gcggagcccg agatggagta
      781 actttccatg gcgtaaatcc aaaagatgaa gaagtagaga tcgattgggt caactattac
      841 caagctgttg atacgtactt aaaggattct ttcgaaaatc ctgaacaatg gccgatttat
      901 ttatttgcat taccagaaaa tcagacgatg ttcaaaaaaa ttgctaaaaa tccctattat
      961 tgtgcagacg ttgcgatttc tgcttctcct gcgcagctat cctttagaga tatcgaagaa
     1021 ggggcgaaga agctgtcaag tgaactcgca gctcaagaat caaaagaata caataaactc
     1081 ttagataaga aattcatcga tcagttagtt gatatcgttc cagctgcgaa agaaggaaaa
     1141 gtgtcacatt tatttatcgc tacgtcaaac ttagctgatg gtttcggtga agatcctgaa
     1201 acagaatatg atcggcgtca agtgttgaat gcattagcag atgatgtgat cattaatggc
     1261 gggaaagtgt ttattttgga tcaaaaagat gcgccagatg aaaagagtct gacagcgatt
     1321 cttcgttatt aaaagaggtt gtgaaatcag tgttttgacc tgagtattaa ggaagaggtt
     1381 gtgacagaag tggtcagctt caagaaataa gaagagattg acaaaaatta tttctcaaat
     1441 ttttgtgaaa tttcggctta tttccgaaga agctacttct ggaacaccgt ttaaaaaaga
     1501 ggttgtgacg tggtcacgac ctctttttga cgattatgat acaaaaaaca tcatttatga
     1561 acaaaatgtg aataacgggc gttcttgaga tatgatttta attgggaaaa acttatcgat
     1621 aaggaggtct ttatgaaaaa aagactgtta tggtgtttgc tgtttctatc cctaagttta
     1681 acaagtcagc cagtttttgc tgaagaaaca cagcggtctc ctattacagg cacacgtctg
     1741 tacaaaaatg tttatgtaca gacaacagat cttgattctg cgattcctga aacagaagaa
     1801 cggtctttgc agccaagatt tggttacttt tggtactgtc cgacctgtga ttatgaaagt
     1861 tgctggcatt tcttacattc tacagctgca gcttttgctt ttactcatac tcagaaatat
     1921 gatggacatc atcctgttat ttatgaggta tagatatgag caaaaaagaa aaactaatct
     1981 cgttgatcgt attggtttgc cttgttcttc tggtcactgt cataggctat cagaaatacc
     2041 atgagaacat cagacctact aaagaatctt ccccagcctc gaccgttgaa acagaaccga
     2101 taagtagtca gaaagaataa tttttttagt ttaaagaaga acatcagagc aaaaaaacac
     2161 caactgaaca atatgtcgtt tattttaaaa aaagttcgtt ttttcacttt atgtaatcgc
     2221 tgagaaaact ttttttaata aaaaaagtcg aaaaactcta gcttttttgt aaacggtatg
     2281 atatactata ggagtagttt ttgttcggta atctgtggta gcttgtttca aataaacatc
     2341 ttccagatat acaccattca agtaattgca aaatatgtgt tgatacacaa ggaggatttt
     2401 tattatggaa acaggtacag taaaatggtt taactcagaa aaaggatttg gattcatcac
     2461 tgcagaaaac ggaaacgatg tattcgttca tttctcagct atccaaggcg acggattcaa
     2521 aactttagaa gaaggccaag cagtaacttt cgatgttgaa gaaggccaac gcggacctca
     2581 agcaacaaac gttactaaag cttaattaac ctatactaaa aaataaacgc ccagacattt
     2641 gcgtgtctgg gcgtttttgt ctattttctt taaaaagcga tgagagtatt tttctgcact
     2701 ttgtagtata atttcaaaag aacattagag gcaggagaag aggaagatat tttgaaacca
     2761 agaacttatg cagtcgttga tatcgaaacg actgggacga atccaaaaga agatcggatc
     2821 atccaatttg gctgcgtcat gattgaatca ggtcggatca cttcacgttt ttcgatcgat
     2881 attcatccag ggcggaagat ttcaaaacaa atccagcatt taacagggat cacgaatcaa
     2941 cgcgtccaaa aagcgcctta tttcgaagat gtcgcccaaa cgatttacaa tcttttggca
     3001 gacaccatat ttgtcgcgca taatatttat tttgattata attttttgaa ccatgaactc
     3061 atgcgatgcg gacttcctag tttgaagata ccaggcatcg atacagtaga acttgcacaa
     3121 attttcttgc cgacggaacc aagctttcgc ttagctgatt tgtcagaaag ccttggattt
     3181 attcatgaga atcctcatca ggcagatagt gatgccgaag tgacaggtca acttcttttg
     3241 ctgatcgaag aacggatgag aaaattgcca atcatcacgt tagagcagat tgcgcgatta
     3301 agtagacaca caggtatgga tacgagtcgt ttcatttatc atgtgattga agaaatgaaa
     3361 gaaaaaccag agccacttga ttcctcatta gaaatcgtcg atgggctagc tttgcagaaa
     3421 aaagaagtgg aactattcac atccgtccat tacggcgagc ggacatatcc aagaaaaaaa
     3481 caagcaaaag aaaagatgtt tggtaaaaca ctgatgtatc gaaaagaaca gaacagactg
     3541 atgaatgcga tctacgacca tttcacaaaa gatgaatcaa aagatttgat gatcgaagca
     3601 gctacaggaa tgggtaaaac aatcggttat ctgttgccat tgagttattt ggctacacct
     3661 gaaaaacccg ctgtcatcag taccgtttcg ctagttttgc agcagcagat catagaaaaa
     3721 gaaattccat tgctgaatca attgctagat cagcccatac aacctgtaat tatcaaaagt
     3781 tatcgccact atatcgacct gcaacgattt aaaggaacac ttgtggaacc accagaacaa
     3841 aaacaatatg ctttgtatca aatggcggtc ttagtttggc tgacgcaaac gaagaccggt
     3901 gatttggatg aattacattt aacaaacttg aaccatgcat tctttgcaga tatcgcgcac
     3961 agaggtactg ggtttttagc aagaaatcag tcgttttacg aacaagactt tgttcgtttc
     4021 ttgcaaaaga aaatcaggca aagtaatttt ttgattgtca atcatgcgtt tttagtacaa
     4081 gaaacacagc gaaaagaacc attgcttcca gctagtccat ttttgttgat cgatgaagta
     4141 catcatttgc cggaaatcgc tgaaaaagta aatgacaaac gattaagtat taactatttc
     4201 cataaacaaa tccagcaatt agaagaacca aatcagctgt ttgaccgaat acaaagctat
     4261 ttaccgaaag aacatgaagc cttacgctgg ttatcgattt atcgagaaga actttctgcg
     4321 ctatgcgaag cacagatttg tatggcagaa ggattaatga gtttagtgag cggcaagcaa
     4381 cccaacggac agtatccaga agaagtcatg atgacaaaag agatgctgga tcagctgcca
     4441 ctggaatctg ggacagccat tgatcacctg cagctttttt atcaggagat tcagcagcta
     4501 cagtcacaaa tgcgtctgac ttttgagatt atccaagaaa gctggacaaa ccagatgcgg
     4561 atcgatttag caagtttata tagcttattt gatcaattag acaagcagta cttgctgatg
     4621 gatcaatggc tggaagactg gcgagatcat ttgatccatt ggttcgttcc tgggaacaca
     4681 gcgcagcaga tgatttttca tctccatgat ttccaagcgg ctatgttgcc gtcgactgtt
     4741 tggtatcctc gatatgatcg tattctttat attggaggaa cgctgaagat cggccctaat
     4801 cgacactatc tctctaagag gcttggcatt gaagcagcac ctcttaaagt gattgcctct
     4861 ccttatgatt atgagaaaca ggcaaggatc tatgtgccaa aagaggcacc tgccgtccat
     4921 caagtcagca atcaagcata catcgattat ttagagacag cacttacagg tctgatcgaa
     4981 caagaagatc ggccaatact tgtgttattc acttctcatg atgttttgca gaaggtctat
     5041 cagaaaatgc atctgtctat gctagaaaaa ggacgagaaa tactagctca agggtttggt
     5101 ggaagccggg agaagctttt gaaacgcttc attttgtcag aaaaaagcgt attgtttggc
     5161 gctgacagct tttgggaagg tgtagatttg cctggagatg ctttgcagat cgttgtagtc
     5221 acacgtttac ccttcgataa tccgcttcgc ccgatggtca aagctaggaa cgcttattta
     5281 gaatcagagg ggatcaatcc tttttaccag gaatccgtac ctaaagcagc tttgaagtta
     5341 cgccaagcat taggtcgctt gataaggacg gagacagatc gtggcgtatt gcttattttg
     5401 gatcgaagac tagtcactgc aaaatatggc gcacggatca tccatgcatt accgaaaaaa
     5461 gtaccgatca aagagctagc tatggaggaa atctatcagg atatccatga attcttaaag
     5521 aaaaatgaag aaggaagtta gaggagctgt cttttttcat aattaaaatc tgctataatg
     5581 aaaaggaatg aagataaagg agcgacgacc cttgaagaaa aaacagacaa aggaaaaaac
     5641 gattaatcgc gtgttacttg gcttgatcgt atttttatta gcggtcatcg tgatcagctc
     5701 gattttttac ttgcgttcca gtcgtccggc ggtgcaggcg aagaaagaag cagtagaaat
     5761 cgcaaagaaa tatgcaaatc ttgaggaaac cgatcatttt tattggttta ctcgaaagaa
     5821 gacatacttt tcactgacag gtacaaatga taaaggaaaa gaaatcgccg ttatcattcc
     5881 taaatctggt gaaaaagtaa aagtattgga tcaaacaaag ggactgactg aaaacgaagc
     5941 aaaacagaca attgctgcgg cccatcctga aatccaaatc gaaaaagctg ctttagggat
     6001 gtatgatgat cagcctgtgt gggaagtagt aggaaaagaa acaaatggga aactgaatta
     6061 ttacctgatt ggctttgaat ctggagatga agtcaagaca atcaagggaa tttaaaaggt
     6121 aaaggaatga atgggatgga gttatcaaaa agaacaaaac gattagaacc gtctgtcact
     6181 ttggcagctg cggcaaaggc caaggcatta aaagcaaaag gcagagatgt gctgagtttg
     6241 actgtgggag aaccggattt tgcgacacca gaaaacatcc aagaagcagc aatcgaagca
     6301 atccgcaatg ggaaagctag ctattataca ccaacagcag gaatcccaga gcttcgacag
     6361 gcaatcgttg attatctgaa gaaaaactat ggacttgtct atgaaccttc acaaacagtc
     6421 gtaacagacg gagcgaaatt tgctttgtat acccttttcc aaacgatcct tgacccgcaa
     6481 gatgaagtca tcattcctgt gccttactgg gtcagttacg gtgagcaagt aaaactggca
     6541 gaaggacttc ctgtctttgt gaaaggggaa gaatccaata gctggaaagt tactgttgaa
     6601 cagctagaac aagcacgttc agaaaaaaca aaagccgtaa tcatcaattc tccatcgaat
     6661 ccgactggaa tgatttatag caagaatgaa cttcaggcaa tcggtgaatg ggcagtaaaa
     6721 aatgatattt tgatagttgc tgatgatatt tatggtcgtt tagtttataa tggcaatgag
     6781 tttactccaa ttgcaacgat ttcagaagca atcaaaaatc agaccatcat catcaatggc
     6841 gtgtctaaaa catatgcgat gacaggctgg cgcattggtt atgcggtggg gaattctgag
     6901 atcatcaatg gaatgattgc tgttgcttcc cagtcaacca gtaatccgac agcagtcagt
     6961 caatatgcag ctgttgaagc attgaaaggt gagcaagata cagtagaaga aatgcggaac
     7021 gcctttgaag aacgcctgaa tacgttatat ccgcttgtgg cagaactgcc aggtgtttct
     7081 cttgaaaagc cgcaaggagc tttctatctt tttcctaatg taaaagaaac gctacgcatg
     7141 tgtaaatatg aaaatgtcac acaatgggta gaagatcttt tagaagaaac aggggtagcg
     7201 cttgtgactg gcgaaggctt tggggcacca gaaaatgtcc gcatgagtta tgctactgat
     7261 cgcgctacgt tagaagaagc ggtacgcaga atcaagcaat ttattgagag taaaagtcaa
     7321 aattaatcct gaagaaattc aaggaatata aaaggagagt catacgtgga acaaattcaa
     7381 atcatcgatt cgaaaaacca tgtgggagaa acagtaacaa tcggcgcttg ggtcgcaaat
     7441 aagcgatcaa gcggaaaaat cacattttta caattaagag acggaacagc ctttttccaa
     7501 ggtgtagttg taaaaagcga agtgtcggaa gaagttttcc aaacagctaa aaatctacac
     7561 caagaaacat ccgtgttagt aactggagaa atccgtgaag acagtcgttc aaaatttggt
     7621 tatgaaatcg gaattacaaa tgtggaagtg gtaagcgaga gccacgatta tccgatcaca
     7681 ccaaaagaac acggaacaga cttcttgatg gatcatcgtc atttatggct gcgttcttca
     7741 agacaacatg cgattatgca gatcagaaat gaaatcattc gtgcaacgta tgaattcttt
     7801 aatcaacatc attttgtcaa aattgatccg ccgatcttga ctggttctgc accagaaggc
     7861 actacggatc tattcgaaac aaattacttt gatcaaaaag cttatttatc tcaaagtggc
     7921 caattataca tggaagctgc tgctatggct ttcggaaaag tcttttcatt cggtccgact
     7981 ttccgtgctg aaaaatcaaa aacacgacgt catttgatcg aattctggat gatcgaacca
     8041 gaaatggctt tcactcatca agaagaaagt ttagaagtac aggaacaata tgtagcattc
     8101 ttagtacaaa atgttttaga tcattgcgaa tacgcattag atgtactagg acgagacaaa
     8161 gaagtcttaa aaacttatac aaaattgcca ttccctcgta ttacttacga tgatgcaatc
     8221 gaattactga agaaaaatgg atttgatgac atcgaatggg gcgaagactt tggttcacct
     8281 catgaaacgt tcattgcgaa ttcattcgaa cagcctgtct ttatcctaaa ctatccaaaa
     8341 gccatcaagc cattctacat gaaaccacat ccaacaagag aagacgtggt aatttgtgca
     8401 gatatgatcg caccagaagg ttacggtgaa attattggcg gaagcgaacg ggcaacggac
     8461 tatgatttct tgctagaaca aatccgtaat cacggtttgg atgaaaaaga atattcatgg
     8521 tatcttgatc tacgtaaata cggttctgtt ccgcactctg gttttggtct aggtcttgaa
     8581 cgaacagtca cttggattgc tggtatcgac catgtacgtg aagctattcc attcccacgt
     8641 ttgttgaacc gaatttatcc ataagaggtt gtgcgcctgc cgttcagctc cgagtcacaa
     8701 ggagtaattt caaaaaacag tttttcatgt ttgatgacta ttgtgacttg tggccggaga
     8761 gcaacgttta tttaaaaaaa agaactgcga actctcgcag ttcttttttt taaatacggg
     8821 ataatggaag tttccagcag acaagagaag aaagcaaaag aaattgctat ttgattcaca
     8881 aggttctata acgaaatcgc aatcaatcaa acatttacaa agaaggtaat tcaaatggaa
     8941 atctgatttg atgatgcttc tgctgttgaa ataaagcagc atctagcacc tgaaaacaaa
     9001 ctaatagtaa cattcgaaga tggagtaggt ccgtattttc agcatgcaat aatccatatg
     9061 taagttcaat tcacccttga aatcatcgga ccagataaag ttatgaccaa tctatcccaa
     9121 gcaatctaga agagatatta gtaaagggac atttactaga agtcttagat gagcctataa
     9181 atatatgttt cgatggcgtc atcagtggtc tgattgacgg aagcttgggt gtgatcgatc
     9241 tctctggacc aaacaacgaa ggaatcaagg ggaatccggc acgttaagaa aaaaatagca
     9301 taaaaaacta gacacttgat gagcgttcat tcaattgttt gaaggaacta ggctggtgtc
     9361 attttttata catatatttt ctctttcgtc ttgatagggc ttatgggata atataaggaa
     9421 gaaaatcatc gttttattca aaaaggagtt tttgacaaat gaaaaaacgt atattattta
     9481 tcggtgatag cattacagac gcaggaagat accgagcaga agcaactgat ctaggtaaag
     9541 gttatccact tttgattgcc ggaaaactaa tctcccaata tccaacgatg gaatttgaat
     9601 gcatgaaccg tgggatagga ggagacaaaa ttgcagatct gcaagtacgc tggcaagaag
     9661 attgtttagc tttacaacct gactatgtat cgattttgat cggaatcaat gatacctggc
     9721 ataatactag cgatcaggct atattcggaa ctgagaaatc tgctcagcaa tttgaatccg
     9781 tttatcgaaa attactaaat gaacttcaaa cagcggaaat ctgcaacatc atcttgatgg
     9841 agccgtttgt acttcctata accgacgacc gtcatggctg gcgtaaagac ttagacccta
     9901 aaatccaaat tgttcgtaaa cttgctaagg aatatggcgc attgctggtt cctttagatg
     9961 gtatattcaa tgctttaggg attgcttatg gtttttcgaa atatacaaga gaagatgggg
    10021 tccacccaac gattggcgga catgagatca ttgcacaaag ctggatagat gcaattgtcg
    10081 cagtcgattt ttttagtgga gataaaaatg agcgaaagaa aagtgaaaaa tgatggaaat
    10141 aaggtgggca gtaaaagaag atttacctca aatcaatcag ctgattactg atcatccaga
    10201 atcattcgta aaagcaaaaa cggcagctga cctaaatgat aagaataaac tattgctcgt
    10261 tgctgtaaca aaagaaaaac ggattatcgg atttgcgcaa gcggacgtgc aacagtcaga
    10321 agaacgattg gatcagcgac catcaggaaa actaatccaa cacacattag taacaaaaga
    10381 aaatacggaa gaaatccaaa aaaaatttgc agaatacttg atgctggaag ctaaaaaaag
    10441 aagaataaaa tcaatttttg cccagtttta atcaaaaaca gtaatagaag gagaaataca
    10501 atgagttgtt taggaaatgt catctggttt atattcggcg gactaatcgg tggtcttagc
    10561 tggttagcag cggggatact ttggtgtatc acgatcatag gtatcccaat cggtatgcaa
    10621 tgttttaaga tcgcctccct ttcttttgcg ccttttggca aagaagtaat ttatcaccag
    10681 tctggcatgt cattgatcgt taatatcatc tggctgatcg tttcaggcct accaattgct
    10741 ctcgcacatg tcgtcagcgg catccttcta tgtatcacga tcatagggat accttttgcc
    10801 aagcaatctt tcaaacttgc tcgattggcg ttgatgccat tcggtgcaga aattgtataa
    10861 aagagaataa taaaggttag ataaagcata gagccaggtg ggatacatgt atttcacctg
    10921 actctatgcg ttttcctgta taagttcttc tatgtgactt ttacgtcttt tatttatatg
    10981 gggttactgt ttatggaaag ttgaaataat tttcattaaa gaactctttc tatatttttg
    11041 aaagcgtagt ggagcttcgt atatctggga aggacatttt gcagggattg aaaaaagata
    11101 ttgtcctatc gaaaaattaa tgaatttaga caaattgcca gcaacaggct tcaaacttag
    11161 ttgctatcct gtcaagatca agaaggcaag tgcgggttgg ataagagcag tggcaatgat
    11221 cgaagagaaa aagaaagaat aagccaaaac atctgtatgg aagaaatttt ccgatgcaga
    11281 tgtttttcat tttttctttc ggataccttg acatatgaat aaccattcat ttataattag
    11341 ttatgaagag atattcatat gttgaggcga ggaggtcatt aaaatgaaaa gttacagatt
    11401 agaaggttta gattgtgcga actgtgcaat gaaaatcgaa aaaggagttc agaaaatcaa
    11461 tggagtcaag gaagcaacgg taaacttcac ttctggaaaa ctaacgattg acgcagagga
    11521 agatcatttg gctacgattg aacaagaaac aaaaaaagta gtgaaggaat tagaaccaga
    11581 tgtcaaagta acagaaatag ataaggaaaa agtatctgaa catggaaatg aaaaaaagag
    11641 aaatacgctg ttccggattt tattttccct tgcaggtatc gctttattgc tattatttga
    11701 ttttaatgaa ccaattcgct tgatagggta tttattgatt tatctgctga taggatatga
    11761 tgtagtgaag aaggcagtga tgaatatcgt caaaggaaaa atctttgatg agaattttct
    11821 tatgtctgtt gctacgattg gcgctatgtt gatcggtgaa tatccagaag ccgtagcagt
    11881 gatgctattt tatcaaatcg gcgagtattt ccaaggattg gcagtcagtc attcaagaaa
    11941 atcgattcgc gaattgatgg cgatccgtcc agaaacagct cacgtgcaga cggcggaagg
    12001 attgatgacc gtgaaccctg aagacgtgct gatcggtcag tttgtacttg tgaagcctgg
    12061 tgaacgtgta ccgttagatg gaacgatcat cgaaggagaa tcattagtcg atacgtcagc
    12121 acttacagga gaatcagtcc ctaaatctgt atatgttggt gaaacagtat taagcggatt
    12181 tatcaataaa aataaaccat ttttagttca agtagaaaaa tcttatgaaa attctactat
    12241 cagcaaatta ttagaattag tagaaaatgc cagcagtaaa aaagcaccag ctgaaaattt
    12301 cattactaaa ttcgcgcgct attacacacc agtcgttgta ggattggccg ttttattagc
    12361 tgttttacct ccacttgttg tttctagagc ggcgttttct gaatggatct atcgagcatt
    12421 gacattttta gtgatctcat gtccatgtgc attagtgatt tccgtgccac ttagtttctt
    12481 tggcggtatc ggaggagcaa gtaaaatcgg tgtattagta aaaggttcaa attacttgga
    12541 gttgcttgca caaactgaga cagtggtatt cgataaaact ggcacattaa cgaaaggtga
    12601 cttttcgatc cagacgatcg atccaacagg aattgatcca aagaaattta tgcaatatgt
    12661 tgcaagtgca gaacagtttt ctacacatcc gattgctcaa tctgttttag aaggctacgc
    12721 aggtccgctt ttaccaacag ctaatatcca ggaatttgca ggtgaaggga tcttagctga
    12781 agttgatggc aaacaagtat tagtaggtaa tcacaaattg atggaaagat ttgaaatctc
    12841 ttttccttca agtcaagaaa tcggtacatt gctttatcta gcgatcgatc aaagttacag
    12901 tggttatctt gttattgcag ataccttgaa agaagatgcc gtagacgcat tggttcaatt
    12961 aaaacaggct ggtgtgaaaa acacagtcat gttgacgggc gactcgaaaa aaattgctga
    13021 tcatatcggg aagcaagtag gcgtggacaa gatatatagc gaattgcttc cagaagataa
    13081 agtgcagcgg ttagaggaaa tcttacaaag taacaataaa aagaccgcat ttgtcggaga
    13141 tggaatcaac gatgcaccgg tgctagcaag agcagatgta ggaatcgcaa tgggtggatt
    13201 gggaagcgat gctgcaattg aagcagcaga tgtagtcatc atgaatgatc aaccatcgaa
    13261 gattgcagaa gcaatccatt tagcaaagaa aacattgaaa atcgtcaaac agaacattgt
    13321 ctttgctatt gggataaaaa ttttggtact ttccttaggt gcttttggtt ttgcatcaat
    13381 gggagatgca gtatttgcag atgttggggt gacagtatta gctgttttga atgccatgcg
    13441 aagcttgcat gtaaaaaata aacgttgatc aatgaagagg acgaatgaac gtcctctttt
    13501 ttttatatcc ttttcttaat gaaaaatgta aagaaaatag ataggattat aaaataacta
    13561 atgtttttaa ttttgc
//
LOCUS       DAFFSV010000077        13489 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFFSV010000077 DAFFSV010000000
VERSION     DAFFSV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.077"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(130..708)
                     /locus_tag="LR110_001930"
     CDS             complement(130..708)
                     /locus_tag="LR110_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4822886.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(728..1396)
                     /locus_tag="LR110_001931"
     CDS             complement(728..1396)
                     /locus_tag="LR110_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822887.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1411..2481)
                     /locus_tag="LR110_001932"
     CDS             complement(1411..2481)
                     /locus_tag="LR110_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822888.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2652..4016)
                     /locus_tag="LR110_001933"
     CDS             complement(2652..4016)
                     /locus_tag="LR110_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL4822889.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(4013..5572)
                     /locus_tag="LR110_001934"
     CDS             complement(4013..5572)
                     /locus_tag="LR110_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4822890.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5628..8108)
                     /locus_tag="LR110_001935"
     CDS             complement(5628..8108)
                     /locus_tag="LR110_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822891.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(8092..8427)
                     /locus_tag="LR110_001936"
     CDS             complement(8092..8427)
                     /locus_tag="LR110_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822892.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8441..9475)
                     /locus_tag="LR110_001937"
     CDS             complement(8441..9475)
                     /locus_tag="LR110_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL4822893.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9565..10245)
                     /locus_tag="LR110_001938"
     CDS             complement(9565..10245)
                     /locus_tag="LR110_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL4822894.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(10415..11374)
                     /locus_tag="LR110_001939"
     CDS             complement(10415..11374)
                     /locus_tag="LR110_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL4822895.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(11595..12698)
                     /locus_tag="LR110_001940"
     CDS             complement(11595..12698)
                     /locus_tag="LR110_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL4822896.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(12920..13390)
                     /locus_tag="LR110_001941"
     CDS             complement(12920..13390)
                     /locus_tag="LR110_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBL4822897.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
BASE COUNT         3823 a         2779 c         2337 g         4550 t
ORIGIN      
        1 taccctgata atcatgagag aataacaaat ttgtatttat gaaaaactac ctatataaag
       61 aagcaagata taacgatctt cgtcatatct tgcttcttca cctaactatt ttctccctat
      121 ataacagctt tacgcttctc ctaatacacc attcaaatat aaatcgatca attgcgccac
      181 atttagcggt tcttccacag gtcttttcga ccgagtgatg taaggtgcta acactaagaa
      241 aaaatgcctt gctgcgattt cttttgaaat cccatttctt agcacagatt ggtttgctga
      301 aaagatttct tcaaaaggtt cacgataatt tttcttccat agaaagaaaa gatgatctct
      361 tgtttccttt gaaagattgc tttcaagatc gtgaagcatc agaaacaaat cgaccagatg
      421 tgtctcaatc aaaaactgcg ccatttttgt caaggttact tgaaatcccg tttcgcttgt
      481 ttgaacgatt tctctcaaag cttttccaac atcttttaag tggttttcta aaactgccgt
      541 atatagtttt tctttatcac cgaaataatg atagagattg ggctgggtga tcccaacttt
      601 ttgtgcgatt tctcttgttg aagtgccgag atatcccttg gacataaaca agtcagtcgc
      661 ttctttcaaa attttttgct gtgtatcttt gttgtatcga tggaccattt ttccacctgc
      721 tttttggtta ttctttttct ttcaatacac catcttccat cataaatatt ctatcgcatt
      781 tgtcaatcag ccgtgtatcg tgagtaacca tgatcgttgc tttatttttg tcttttgatt
      841 cttttgccaa gatatcgact acttctcgtg ccttttctga atccaaacta gcagttggtt
      901 catctgctag aataatggac ggatcattgt acaatgcacg agcgattgcg accctttgcc
      961 gttctccacc agatagttct tctggatact tgttgatcaa cttatccacg ccaagctgct
     1021 caaataattc gtttgcttct ttttgacgat gttcatgttt aatcttatct actaataata
     1081 actgcttttt tactgtcaga aaaggaacca gattggaagc ttgcaaaata aagccaattt
     1141 ctttaaaacg tagtttaacg cgttcgcttt ctttttgatt actgaattct tgatcattga
     1201 taaatacaga gccactgcta ggcgtctgca atccgcctgc caaagtcaaa aaagtacttt
     1261 tacctgaacc agaaggaccg atgattgcaa caaattcccc tttatcgata ctcatggttg
     1321 tttctttcaa tgctttaact acttggtcgc catcttggaa acttttttct acttctttaa
     1381 attctattgc tttcatgttc ttcctccttc ttatccaatt gccttcaacg gatcgatttt
     1441 tacgattgtc cgaacagaaa atagtgcgcc taacacagct actactaaca tcaacaagct
     1501 aatgacgcca aaaaacagcc aattgctttg gaatggaacc gcatcaggta gaacaaggga
     1561 tgttcccact gttcctaata gaccaagaag aataccaaca aatgataaaa tgaatgtttg
     1621 tgccactaca gaacgcgcaa taaatcctcc tgtgatccct tgtgctttca ttacaccaaa
     1681 gatattaatc ttttgcatcg tcagtacata aatgaaaatc ccgatcacaa tagccgcaat
     1741 aacgatcaaa aatccgatca taaatccaaa agtcaatact tgcgcattat atccaggtaa
     1801 ttcattgatg aatttagaaa ttttgatctg ttctaaatct tcaggtaaat tactgatttt
     1861 acctctgaca actattgcgt taatccgtgc attttcactt gtatcttctt tttcgaagcg
     1921 gatttcttgg taagcattga tggttgtata gagaactggt gagacattga atttggcatg
     1981 atccgtaaag ccagttagtt taaacgtctt gtcactacca gataacttaa ctgtatctcc
     2041 tatggctaga ccatattctt ctttcaaact aatatcgcct actgcctcat catcgttatc
     2101 aaaagctttt ccttcaacaa gattgggcat gatgaattga tttttatcga taccgaagaa
     2161 gctaacattg atttttcctt cttctgtact gtctttacta gtcacgactc caggtgtttg
     2221 ggcaagatac gccactttcc caccttcgac ttcttctgct atcttcttag taatcatgga
     2281 catccctaga tttgaatttg catctttcga aagcacaatc gaatctgcct cccacttgtc
     2341 gactgctgtt cgattatctt gagctaagcc ataagctaac ccagtcaaaa aaaataccag
     2401 gtaggcgatc aaaaacatca cccctgcaac taacgcataa cgtaactttg aatgcataat
     2461 ttcatttaat gctaaaaaca tttaactcct cctcctgaaa taatcatttt atacaacaat
     2521 tttatcactt gataaatttt tggcgaatat tatgctcaaa aaattcttcc gctatttcat
     2581 tatatgaatt ttttattctc gattgattaa tatttattat atccccacac aataaaagac
     2641 cgtgatattt ctcacagtct tttatctact ctattgctat ttgctctctt agctagtact
     2701 ttaaaagcta actcattgag gtagtgaaaa cgagaggtgt attcagacag aaatccaaaa
     2761 tcctcaactg ctagctgtgg aatcgtagtt ttgtgtttca cgatatccgc aagcaggttc
     2821 aaatctgata ataagaattc ttggcttatc agctgtccgc caattatttc taatgtatcc
     2881 ggatggtaga tcagctttgc atttactgtt ttgttttctt cgtcgtattg tggaaataga
     2941 tcaaattctc ctgaacacga accggtattt tgttccaaga atggcgcttc atcttctgtc
     3001 agccctacag ttcctaaaaa gtagtctata atgactgctg ctgttatctt ttgtctgagg
     3061 tcgtaagcgg ttggctgacc actcaaagtt agcgctactg ctcttgccat atgtacagct
     3121 cgagtaacta aaggtaaaaa gagatgccgt ttatttttgt caaaactgac tgggacgata
     3181 tctccaatcg catagatgtc cggatcactt gtctgcagat attcattgac tatcacagtc
     3241 tcatcctcat acaaagccac tttttctttt aacaatgtcg tattaggacg agtttgggca
     3301 gaaaaaatga ccatatctgc ctttattgtt tgagacagaa gttgtacgga tactactttc
     3361 tcttctgatt ttttccaatc gatcaagaat tcatttggat aaaaatttat ttgcttagtt
     3421 tgcagctgat tttgaaaact gttgatcata tccttatcga aatatcttga taaaacattc
     3481 ggcatataat caactagatg aataatgtat cctttatttc tcaaagcatc tgctaattcc
     3541 aatccaatgt aaccagcacc gaagatcact acttcttttg cttcttttaa agcggcaagt
     3601 acttgcttag attgcactag atttttatag gtgtagacat tttcagcttc aatttgttta
     3661 tacaaggtga caggagagga acctgtcgct acgattaatt tatcataggt tatggtctcg
     3721 ttttttcctt ctttggatgc gaggatgagc tgatgtttgt ctggatcaag ttcagttact
     3781 ctccattcgg tgatcagttt tgccccactt tcttgaagag tccgcatgtt atgggcaact
     3841 tcagctaaac tacgtatctt tccttttaaa taaaggttga ttccatttga aacatagcct
     3901 agtccatctt tatgatcttc atcgatcagt aaaacttctg ctttcggttg aagttttctt
     3961 agatttaggg cagctgtgat gccttcatgg gaagcaccaa tgatcacgat tttcatgttt
     4021 tattcatcct taaattcaat ttttctaaat aacattcttt taaaaattgg ttcaaatagg
     4081 ggaaatcata attatattcg ttgctagtga atacaaaaac tttagcggac ttctgtgacg
     4141 ggtactcttt caattggcta gtcaaaatga tatcatactg cttttctttc tcgtattcga
     4201 ctaaatcata tttgatcgta ttcagcaatt gttcctggat gatttctttc acgatatccg
     4261 ccattagcct ttcataagga agatcataag caatttgcag acgtaattct aactgcttga
     4321 tcagcgtccg taaaatagat gcatagcgag caaataattc aaagcgggca ttttcctcgt
     4381 ctacttcagt aaaatcaaca atagcaaatg tttgtctgac caatctttca cataaaacca
     4441 ggaattgttt atcttctact tcagttaacc gctgttttaa tccttcttgg ccaaaaatat
     4501 tcatccatcc tctaaaatag gcaaaatgag cgtgattttg catcactgtc aacgcaatct
     4561 tttgcgaaat ctcttctgga aaaacataaa aagagaaata ttcttctatc gattgtttaa
     4621 acgttcgatt caattgagct actacgtcgt ctttgtattt ttcgcttatc aaaaactcct
     4681 ggacatgcga gttatgtgta tctaatgtaa agttactgat catgaaaaga taaaaaacca
     4741 acgtttcatt ttgattccaa ggaaaggcat attgaaagaa gtaacgggtc aaaaatgtat
     4801 ggaggtccaa taaaaattgt gtcgtttgaa acaatccaac aggaagattg ctataatctg
     4861 gtttggattt tttcgtacag cgcattcgct tcttcataat ggaaagccat atttcaaatt
     4921 tcaatcgtcc ttcttctgta atagcaaaac taaactcttt ttgcagcaga tctaaaaaag
     4981 tgtttcctga taaatcactg tattcttttt tgttttcaag atagggacgt ccaaaccaaa
     5041 ataattggaa ataaaaataa cgaatttgac tttctggacc ggtcagccgc ccgcttttta
     5101 ttttcagatg aaactcattt aaagaatcat ttaattcctt cactctgcga gacaaagtag
     5161 ccgaactgat attaagttcc atgacaagtt tctgccgatc aaatgagcca ttttccaaca
     5221 gataaagaag tatctgatat tttgtagaat agcgcaagta acaatgataa taaacatcga
     5281 ctgctgctat atttccatgt ctgattaata aaatattccc ttcttggata acaatttcca
     5341 attgtttgat attttccaag ttagcttgta aataaaacag atcttctttc aaggtagccc
     5401 gatcaactga taagcgggta agtacgttat taacggaaac ctctcctttt tcctctaata
     5461 aaagttggaa aagtttgatt tgccgttgtt catttttttc aagcaaactg aaaagttcca
     5521 agtccactcc ttcaagcatt tctgatttta acagcagaag tttccttctc atccttgaaa
     5581 aagaggatct gtgacagtta tcacagatcc atacaaattc taatcactta gctggttggc
     5641 ccagctgtca gttcccatgt gaccatgcct tgatacgttg cgccaggttg agcattcgta
     5701 ctgctattta cctctaagag taaaccatcg gtatcgggaa agtcaacgag aacctctcct
     5761 ttagctgttc catcactctc ataaatcaac gtgttttgac cttgctgaat caattgcttt
     5821 gatccagaat gattgaagta taattttgtt ccgcttaatt cttgaccttg atcattctta
     5881 aataaagaag tcaatgtacc tgtcagttgc caaggagacg tgcttggaag ccgagtatcc
     5941 cgcacatcaa aagaaaaatc gccttttgat tcataataac ttgtcttcaa cggtttgggg
     6001 agatcgttac caaatgtaaa atcatctggt acattaacta atgttaattc ttgtttgggg
     6061 acaagaattg ttttagcaga tgtttggaca actgggttag gaagcttagc accatctgct
     6121 aatgtaatat tgctggaaag tacagctgcc tttgtcagta tgctgacgga ttgtaaagaa
     6181 gtatctacag tatttaattg atagttgtaa gtaagatgaa tggtatctcc tattttgctc
     6241 aatagatctg ctgggacaga aactccttgt tgaatgccgg tatctgcgat agttgtacca
     6301 tttaatttta aggaaccatt gatataagta gtattttcag gaagtgagat cgtgattttt
     6361 ttgtcggtta tcgcatcccc agattttgtt aacgtgaatg tcacattttc ttgaatcgta
     6421 tctcctgata gagcagacag gggatctcct gtctcaggtt gtctgctaag gttttttagc
     6481 tgactatctg cactcacaga tggaaaatta gccactgtta tttgaggaag agtggtgatt
     6541 ccagcttgtt cagactgcca tttgtacgca aaagacggat acatgttagt aagtacatcg
     6601 tccaccaagc gataatcaaa ataatattgc cctgaagcca atgcaggtag tgtagaagta
     6661 gctgtattta cacccccgtt atttgattgg atcgtttgga aaagttgatc tgtccatcct
     6721 gtcgctaatt tttgggttcc tttcgaataa agcctgtaat ggatcgaacc cttttttagt
     6781 tcagttgctg accaattggc cgtgacttgg tacgatgcag aagaaaggag ctttccccac
     6841 tgactatcgg ctgttaagtt tgttggtaaa accgttccgt ctagcttgta ctgctccaat
     6901 acctttaaag caacaggaga aagcaatccg ccttgcgcat ctttcccact gtacacgata
     6961 ttggctgtct gattcaatcc acttgtgctt gctggagtat gagtcagtat tgctccttgt
     7021 gtaacgtttt ttcctgtaaa atagttgact acgcgatcaa agcgactaaa tggcaactga
     7081 tcaaaacgtc cttcagtcag ccccatcgtc aatgtccgac tattggtaga gcctggaagg
     7141 ttctggttat ttccatccac gtattctgtg gtatcagcaa atgtattata ttttcctgtt
     7201 gaatcacttg gtgagatgta actattccac gtatttatct ctgcacttgc agaaaatgca
     7261 tatgctcctg gattcaagtt tccgccagtt aatttaggat aaaatctacc tgcagctgga
     7321 gctaaaatcg aattgaacat acgatcttgg ataatattat tcgaatcata aaaatcaaca
     7381 gaaaaaacgg cctttttaga catagaaggt tgtaatttac ccttggtcaa aattttgttt
     7441 ggactcgtat catatttatc tccgttttct gtcatcttcg cgacattagc tgtaacatca
     7501 aattgaagcg ggatatcgtc cacgttgaat tcgcctcgcg taataatagc aataatatct
     7561 gtcaatagag tatttgtatc ccacgacccc atcccaaaac gcaaggtatg gcttcctgct
     7621 gataataaac ttccgctagt aaccttgatg ggggcaccgg caatatacaa gtaggcgttg
     7681 ttccagtcaa ttgcttttaa tacatcactg cttgatacat gttccggcaa attgatgatc
     7741 gccacaaatg caaatccttg tgcagtgata ttcaccaaat caagtacatt tgttagtttc
     7801 acatggcttt cccaatgaaa acggaccgtg ccacctttcc aaggatcatt tgaaccatcg
     7861 ttcaaataag tagcagtcaa tttaggcaat tggttagccc cagttagttg gacaagcttc
     7921 ccgccattga ctgcactacg tccaccattt ggaggtacga tattttcata cgtgtatgga
     7981 tcataagaaa tggcaggagg cgttgctcca acagcttctg ccgaaggtga aaagataaat
     8041 atacttaaaa caaaaagcga agtaaaaaag aaggagcgaa aaaatgaaaa tttattttgt
     8101 agcaacatgt tcatcctcct tttcctttct tctccataac aacaaccacg caagaagcca
     8161 taattctata ccaatgatca aataaagaaa acggatcttt tgttcattcg tctccgggaa
     8221 tacgccatgt tgattagatt ctttataaat cggtagctgt gtctgctgtt ttgatgcgcc
     8281 attgactgaa ccacttggat gcggaattgg acttagatca taaaatgtaa tcgtacctga
     8341 acttttggta tctgcttgga ctggctcttg ccagctgaag aacagcaaac ttgcaataat
     8401 ccctcccaat aagatcgatg ttttcaacga aatcacctca tcatgttgtt tctccttttt
     8461 tctttttctt gtggacccac aaaaacagca aaatcaacca taacactaga actgcagcaa
     8521 ccaatcctgc ataaagccat gggaaagatt gttcttgtac tgcaaaaacg gaattttcat
     8581 ttactttttg tgctttttct tttttgatcg taaagttttg tgtaaatcgc cattcattac
     8641 tgtcgcctga ttttgctatg atttctgcgc ggtaatcgcc ggcttttgct ttatcaccag
     8701 ctaaatcaag attcaagtga aaattcgaat ttggtgcaac taaaaatgca ttagacgtat
     8761 tttcaatata aagctgattt tcataaaaga tcttgctcgt aaatatcaaa ttgctgataa
     8821 tcctaggctg aggattttgg aaacgaagct gaacagttgg ctttgcgttg acctcgtcca
     8881 aatctcctga taaaagtttc agatccggtt ctatcgtctc ttgactttct tgtaaaacga
     8941 ctgcgagatt ataagcaata cgatttttga tttgtgctcc ttctgtagca ttttctgttt
     9001 cagcttcttc tttaggtgaa acatgaatac ccgctaatac acgtccctta aagctttttg
     9061 ctgggatttt cacttctata ggtacagttg tttctgcatg tgctgataca ttgatttcct
     9121 ttttcgcaat gagcaaaacg tcacggacat caaacggcaa tgtctcatct aacgttgggt
     9181 ttttttgact ataatcgatc gttccaccat cactcgttac cgccggattg acagagacct
     9241 caaatgttcg ttcttcatct gaagaattgc tgataattac ttctagtgtc tgattttgtt
     9301 ccggtgccac taataaatca aaataaccga ttgctttatc tatttggttc tctggaaata
     9361 ccggtttgat tccaaagtct cctgctgaat cctttgcttg gctgattgac ggaaaaatga
     9421 aaaatagact taaaatagaa aaaaacgaaa caaacagtcg tttactttga ttcattgtcc
     9481 accacatcct ttcaacaaca actgcggtaa aaaatccttc acctatagta ttttcaccgc
     9541 agtttgttga atttatttca tactctatgc tactgggcca gccacgaggt tccaagtcaa
     9601 atcagccgtg aatgttccag ctgctgcttt cggcactttc agtgtgatat ttgctggatc
     9661 ataaacagaa agccatgttc ccagaccttg atcttttgct gctgtaaata ttggtgtcgc
     9721 tgcacttgct ttactcaacc cagttacagt ttgactagtt ggtccagtgg attcgttatc
     9781 tgacgtactt ttaacagtgg atgttccaaa atctaattca gcgccttgca aaacttgtga
     9841 accgtttaca aaatctgaaa ccgaaactgt cacgttccac ccttgtgctt ggccatctgc
     9901 acctacacct cgacgatctg atacttggag ataaggtgta tcgttttttg aagcgtctac
     9961 ttgatacgtt tttgttccgc tttcaatctc gtgtgtacca aatggcaatt cgggaactac
    10021 atccagtgtt aaaggacctg tctggccagt tgctggattt tccggatctg ctggctctac
    10081 tggttttgaa ggatcagtag ggttaagagg ctccacggta tttgttggtt cagtaaaggt
    10141 aatatctcct tttgaagtcg cttgaccctc agcaaaagct ggtgttgcca agccgcctag
    10201 aagaagtgtt gaaagtaata atgtcccaat catgttcttt ttcataataa atcactcctt
    10261 tttcttagtg acctaagtat agtagagaca gtatgcaaaa aatcctcatg aattcatccg
    10321 cttacttgaa attatgaccc tttttgtata aaagagaaaa aaacaataaa atcgtttaca
    10381 tgaatattct tcctttttta ttaagtttcg tttgtcaaat taagttagac aaatcatctt
    10441 ttgtgacgta actcaaaaat acttccattg gtgtccgata gtgtaatgat tttctaggaa
    10501 tatgatttcg tttcgatgcc accgattgga taaaggtttc agatacacca ttaaaatcca
    10561 tagctttata caaaccatct ttacgtaata agccatttga atgttcattc aatcctcgtt
    10621 gagaaggtgt tcctggatcc gcaaaataaa ttgaaatatc gttggtatta ctaatctttt
    10681 tccaatttga gaattctttt ccacaatcaa atgtgatgga tttaaataag ttttttggga
    10741 cacaacgaaa ccattgattc agcctatttt caatgtcttt agcctgtctg ccttcgggtt
    10801 tcaaggtaat gatgacttta gatagccgtt caactaatgt gatgaccgcg cttttatgtt
    10861 ttccacccac gatagtgtca ccttccaggt gaccaaattc atgatcaaac tgggtatagt
    10921 ctgtctttct gtctcggatt gaccggcgaa acccttgttt tcctcttttt tctttatgac
    10981 cgtttggttt tctttttccc ttcattggta aatcgtgagt gtcaaaggtt ttatctttaa
    11041 acattcgata cagtgtgcgt accgaacaag caataggaaa ctctgctcga ccaataagga
    11101 catcaggagc ccacccttga acaacctttt tttgaatgta ttcctgttgc tcatcaggta
    11161 aaacaatcgg atgtcttccg cagttacgtt tgtttttctt atattgttta tagtagctca
    11221 ttgcgcttcc accagattta agaaaacgat agaccttata aatagtttgt cgagaacgtt
    11281 gtaacagatt ggctactttt gaaacaggtt gtgattgatt gaaataagct tctatcatta
    11341 cgagttcagt tggtgtaaga tggttatagg tcatttgtac tcactcctta taattctttc
    11401 gtcggaacta ttttgagtgt agcacaaatg acttttttta gttgtctagc ttaattttac
    11461 aatcggcgaa gtatgaaaaa atatttcaat tttatttttt cgtttcaaat tattctatta
    11521 gtgacatcct tgaactataa aaataggagt acggcatcat ctgccgcact cctatttttt
    11581 tcgtcatttg tttcttaaat aaaacgattc tcacgcaatt ttattttttt gatatcttct
    11641 actgtttgtg ttccagctaa ttgcatgacc atttccaatt ctgttttaaa gaaatcaaac
    11701 acttgatgga ctcctgtact tcctcctaaa gacaatccat aaataacggg tctgccaata
    11761 gcaacaagat ctgcacctga tgcgattgct ttgaaaacat gctgtccgcg tcgtacaccg
    11821 ctgtcaaaca cgattggtac acgtttatct actgcttctg ccacatattg caacgaatca
    11881 aacgctgctg gaccaccatc tagttgacgg ccaccatgat tagataccca gattccgcca
    11941 gcaccagatt ctaatgaacg atagacatct tcttctgact ggacaccttt cacatagact
    12001 ggcaaatcag aatgagcagc aataaattct acatcttttg gactaagctt ctgtttggaa
    12061 gatttgtaca cagcatccat cgtttggcct actcctgatt gataagcctg aacgatcggc
    12121 attggcagtg ggaaggtaaa cccatttctg cggtctgttt cacggtttcc gccaactgtt
    12181 gcatccgctg tcaatacgat tgcttttgct ccattacgct tggccacttc aagaatatcc
    12241 aaattgatcc catcatcttt gctcatataa aattgaaacc attgcggtgc tttctccccg
    12301 cctgcttctc ttatctcttc taatgtacaa gaagcgtaag aacttgctgt atagatcgtc
    12361 ccaaaatctg ctacaccttt tgcagaagct ttctccgcct tcacatgagc taagccatga
    12421 gcagcaactg gtgccatgat gattggtgca gtcagcgttt ctttgtcaaa atgtgtagtt
    12481 gtatcgggta attctacatc tcttaataca tgaggaataa tcagctgatg attgaatgcc
    12541 cgttcatttt cttgataagt aaataagtcg ccagcaccac tgctgatgta accataacca
    12601 ccagctggaa tcacttgtga agcggctatt tctaaatctt ccatattgat aaaatccact
    12661 attccttcat ttgtacctgc ttgatacgtt ttttccattt caatgccttc cctctataaa
    12721 aaattgaatg aataatatga tagcgtttac acactttata ttactcttcc gtattgatac
    12781 aggcaaataa tctgtttaca tttcagtcat tccagttgtt actactctga aagaaatgtt
    12841 tagaacacaa atagataaaa aacgatggga ttttgacaaa agtcgtgtca aagtcccttt
    12901 tacgaaatac tgtggatgcc tattgaaata agtgcgggtt gtttctttgt atttcaatcg
    12961 tttcttgcca ttcttcgttt aattttggat taccgttttc ttccttgaac gcataaggtc
    13021 ccaatccaaa aagctcagtt agtatttgat tcacgattcc atatagctcg gtgtgatcgg
    13081 acacatgatt attgaagatc atctcattat caccacgttt gaatctcaca ttcgggctct
    13141 catgatgaac ctctatgcag ttttcccctt tttttaactg acggatttct gaaaacatct
    13201 cataaatatc ttctccaata aaaccttcag caagcgccag actgtccaca agaccgccta
    13261 ctgtcaactc agagattatc gctttgttgg caccaagctt taatgtatag cttgttgctt
    13321 ctactacgcc gcatgcattg caaccacctt ctgatagttc gctttttctg atcaattgaa
    13381 cttctttcat tttctgttcc tcctcttagt tttctactta tgtatttttt atcacctgac
    13441 gtaagtgtat tttatcgtca cgatcacgtg atttattctt cagagattc
//
LOCUS       DAFFSV010000078        13482 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFFSV010000078 DAFFSV010000000
VERSION     DAFFSV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.078"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(164..388)
                     /locus_tag="LR110_001942"
     CDS             complement(164..388)
                     /locus_tag="LR110_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBL4822898.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="LR110_001943"
     CDS             complement(586..2166)
                     /locus_tag="LR110_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBL4822899.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="LR110_001944"
     CDS             complement(2567..3943)
                     /locus_tag="LR110_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL4822900.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="LR110_001945"
     CDS             complement(4071..5189)
                     /locus_tag="LR110_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL4822901.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="LR110_001946"
     CDS             5331..5765
                     /locus_tag="LR110_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4822902.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="LR110_001947"
     CDS             complement(5843..6376)
                     /locus_tag="LR110_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBL4822903.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="LR110_001948"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="LR110_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBL4822904.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="LR110_001949"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="LR110_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBL4822905.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="LR110_001950"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="LR110_001950"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL4822906.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="LR110_001951"
     CDS             complement(10101..10679)
                     /locus_tag="LR110_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBL4822907.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="LR110_001952"
     CDS             11001..11471
                     /locus_tag="LR110_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBL4822908.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="LR110_001953"
     CDS             complement(11579..12805)
                     /locus_tag="LR110_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL4822909.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3954 a         2718 c         2248 g         4562 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gc
//
LOCUS       DAFFSV010000079        13212 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFFSV010000079 DAFFSV010000000
VERSION     DAFFSV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.079"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            147..1016
                     /locus_tag="LR110_001954"
     CDS             147..1016
                     /locus_tag="LR110_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBL4822910.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="LR110_001955"
     CDS             1225..2250
                     /locus_tag="LR110_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL4822911.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="LR110_001956"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="LR110_001956"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBL4822912.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="LR110_001957"
     CDS             3333..5036
                     /locus_tag="LR110_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4822913.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="LR110_001958"
     CDS             5038..7071
                     /locus_tag="LR110_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL4822914.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="LR110_001959"
     CDS             7082..7522
                     /locus_tag="LR110_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4822915.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="LR110_001960"
     CDS             complement(7722..8849)
                     /locus_tag="LR110_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822916.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="LR110_001961"
     CDS             complement(8852..9529)
                     /locus_tag="LR110_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822917.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="LR110_001962"
     CDS             9793..11559
                     /locus_tag="LR110_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBL4822918.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="LR110_001963"
     CDS             11574..12011
                     /locus_tag="LR110_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4822919.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="LR110_001964"
     CDS             12024..13175
                     /locus_tag="LR110_001964"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBL4822920.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAFFSV010000080        13117 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFFSV010000080 DAFFSV010000000
VERSION     DAFFSV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.080"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            152..1093
                     /locus_tag="LR110_001965"
     CDS             152..1093
                     /locus_tag="LR110_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBL4822921.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1162..1893
                     /locus_tag="LR110_001966"
     CDS             1162..1893
                     /locus_tag="LR110_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBL4822922.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2037..4217
                     /locus_tag="LR110_001967"
     CDS             2037..4217
                     /locus_tag="LR110_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBL4822923.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4237..4719
                     /locus_tag="LR110_001968"
     CDS             4237..4719
                     /locus_tag="LR110_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBL4822924.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4735..5496
                     /locus_tag="LR110_001969"
     CDS             4735..5496
                     /locus_tag="LR110_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL4822925.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5627..6961
                     /locus_tag="LR110_001970"
     CDS             5627..6961
                     /locus_tag="LR110_001970"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBL4822926.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7268..7753
                     /locus_tag="LR110_001971"
     CDS             7268..7753
                     /locus_tag="LR110_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBL4822927.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7746..8429
                     /locus_tag="LR110_001972"
     CDS             7746..8429
                     /locus_tag="LR110_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBL4822928.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8555..11200
                     /gene="polA"
                     /locus_tag="LR110_001973"
     CDS             8555..11200
                     /gene="polA"
                     /locus_tag="LR110_001973"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBL4822929.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11245..12081
                     /gene="mutM"
                     /locus_tag="LR110_001974"
     CDS             11245..12081
                     /gene="mutM"
                     /locus_tag="LR110_001974"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBL4822930.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12045..12674
                     /locus_tag="LR110_001975"
     CDS             12045..12674
                     /locus_tag="LR110_001975"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBL4822931.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12726..13058
                     /locus_tag="LR110_001976"
     CDS             12726..13058
                     /locus_tag="LR110_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL4822932.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
BASE COUNT         4430 a         2319 c         2893 g         3475 t
ORIGIN      
        1 gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac aaaaaacttt
       61 ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc tttatactgt
      121 ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc tttattacaa
      181 ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct tttgcaaatc
      241 tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta ctgtgtctgg
      301 aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc gatttgctga
      361 tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt ttgtaagata
      421 ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc aagtgacagg
      481 tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga cagtggtgat
      541 tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg tacgcccaga
      601 tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag aatttatgga
      661 aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt ctggttggct
      721 tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta cttatggtaa
      781 ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg atgaacggat
      841 cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt ttgacggaac
      901 accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga ttcttgcttc
      961 cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga aacatccgcg
     1021 caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc ggtttacagc
     1081 tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg cgctatacta
     1141 gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga tgtccatggc
     1201 aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca tcagcttgtt
     1261 ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt attaggaaag
     1321 caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga atattttttg
     1381 caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg tggaaaagaa
     1441 acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac tgaaatctcg
     1501 atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg ccccttgtat
     1561 tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa gaaagactgg
     1621 cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga agggaaaaac
     1681 aatacaggta agacaattgt atttggacat acgattacac caatgctcca tggagatatg
     1741 cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg ggctgtattc
     1801 ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga tattgaatat
     1861 cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa ggagctgtga
     1921 ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta gtttaaccca
     1981 ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc attttaatga
     2041 cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca gactatcgtc
     2101 ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt ccttttatcg
     2161 cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt gaaatcgaag
     2221 agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt cggctaatcg
     2281 atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc gtgaaaatgc
     2341 aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa gcaacgattg
     2401 ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca gatggagagg
     2461 tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct gccgaagaag
     2521 cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca aaatttcgga
     2581 cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc aaggatgagc
     2641 aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc gtccataaaa
     2701 tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt ctgaaagtat
     2761 tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata gtcaaaaatc
     2821 ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat aaacgcttta
     2881 ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca gatgagcaag
     2941 caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca ctaaaaggta
     3001 aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca gtcgtagatg
     3061 cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca gcgcaagcaa
     3121 aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa gtagatatgg
     3181 tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct gagcagttga
     3241 aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct tctgtttact
     3301 ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa agaagtgctg
     3361 tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc gatcctaaag
     3421 ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct gaacaattgg
     3481 attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca gcaagcccgc
     3541 agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt gtcatttatc
     3601 gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca cgcttaggac
     3661 caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa aacattttgg
     3721 acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta acttcagtcc
     3781 aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca cgattatccg
     3841 tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat attttagctg
     3901 ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta ttacgtacag
     3961 atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg gtccgtaatg
     4021 tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt gtccatatat
     4081 cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta ggagatatcg
     4141 taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg acgatgcttt
     4201 ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga actgcagcag
     4261 ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca agcaatgttc
     4321 aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca tttagatttt
     4381 aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat caaacatgaa
     4441 ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga tatggatttc
     4501 aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac gaagaaacag
     4561 ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg caagagaaga
     4621 atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg gattgagaca
     4681 aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag gcctttggaa
     4741 aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt agccgcacct
     4801 gaagagggaa attttgagga actgacggca cctacactta ttttagctgg aaactgtctt
     4861 cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga tcaattgttt
     4921 ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga aaagcaagga
     4981 tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct aaaagaagtc
     5041 tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc tggagagaac
     5101 gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa aatcttattg
     5161 atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt atggcaaaat
     5221 gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca cttttctgaa
     5281 gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata tttccatgcg
     5341 ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg tccgaaagga
     5401 aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta caccagaatt
     5461 aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc gaatcctcct
     5521 ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga gcgtgttgaa
     5581 tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg aaaatcaaaa
     5641 taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg ctcttgtttt
     5701 acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct ttacacaaag
     5761 agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg taaaaccagg
     5821 gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc agcgagcaaa
     5881 agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta tccaaaatta
     5941 tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac tgctctctca
     6001 tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag gacatggtga
     6061 tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt tccttgccta
     6121 ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt attatacaag
     6181 tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa aagctatttt
     6241 tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga tttattatta
     6301 tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa cttctggttc
     6361 tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac cagcattcgg
     6421 taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg aaaaactgga
     6481 tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc gattcagtga
     6541 aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg cagagatcaa
     6601 agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg cagttttcca
     6661 accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag cacttgattt
     6721 agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac aaggaaacgt
     6781 taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca aagaaaataa
     6841 tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg ctggagatgt
     6901 tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa atgtactgta
     6961 atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa aaatcttgcc
     7021 aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc tgcggtaata
     7081 agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt attatttgat
     7141 gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc aagggagcag
     7201 aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa actggagggt
     7261 ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg aaatcgagga
     7321 aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc tgtatttggg
     7381 attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa aaacagaata
     7441 tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg caatttcaaa
     7501 gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta ttgaaccggt
     7561 tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata agatgaagga
     7621 tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt tggatgctgt
     7681 cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa gtgaaggtga
     7741 attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat gcaaaagttt
     7801 atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg gctctttggg
     7861 tatttccttt gcaattagca acttttatca ataattttcc actaggcttt gtgggtctgt
     7921 ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag aatccgagct
     7981 tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta gcggttactc
     8041 ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact ttgatgttca
     8101 tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt ggaagagcag
     8161 gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc attttgagaa
     8221 gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca gggattacag
     8281 cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca gcacatagtg
     8341 gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg ttcttggcgt
     8401 ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt tggggctata
     8461 ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt ctttgttaga
     8521 atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat tattactcgt
     8581 tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc ttgaacgttt
     8641 caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga tgtttgaaaa
     8701 tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag gaaaaacgac
     8761 ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac ctggcgaatt
     8821 caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac agtattacga
     8881 attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag tagacaaaga
     8941 tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag ctagtaaaga
     9001 agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata caccagaaca
     9061 tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga agggactagc
     9121 cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa cagctatcaa
     9181 gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg aaatgaagca
     9241 aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat cgaaaaaatt
     9301 ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga agtatgaagg
     9361 gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc agttcctttc
     9421 taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg aagtcgttca
     9481 tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa tgatgggcga
     9541 caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga aaatttatgt
     9601 cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca ctgatccgac
     9661 tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc gctatactgg
     9721 aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg atacgaatga
     9781 aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata tccaatcaga
     9841 tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg aactcttttt
     9901 tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat tgagccaaga
     9961 actggcagat aaaaaccaag aagatctttt ccggaaaatg gaactgccat tgtctatcat
    10021 tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac aagaaatgaa
    10081 gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag aagccggcga
    10141 agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa aaatgggatt
    10201 acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac tggaacagct
    10261 tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg caaaaattca
    10321 atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag tgcatacccg
    10381 ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta atttgcagaa
    10441 catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc ctagagaaaa
    10501 agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac ttgcgcatat
    10561 ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc atgccagcac
    10621 agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata tgcgtaggca
    10681 ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac tttcacaaaa
    10741 tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg aaaaataccc
    10801 aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag gctacgtcga
    10861 aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt acaatatccg
    10921 ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg ctgatatttt
    10981 gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc aagctacgat
    11041 gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac ttgaatcatt
    11101 agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac cactgatcac
    11161 tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta agacagagca
    11221 atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac cgttcgtaaa
    11281 ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt gtggcccaag
    11341 atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga aaccatccaa
    11401 tccattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga actgatctca
    11461 cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat cgataaacat
    11521 acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga tgtacgaaaa
    11581 tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg aatcatgaaa
    11641 ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa tggactgaaa
    11701 aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac tggtctaggt
    11761 aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca accggcgaac
    11821 acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt tttggatcgt
    11881 gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg agaatcaggc
    11941 ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat acgttgtgga
    12001 acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc gaattgtcaa
    12061 aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata gctaccggta
    12121 aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat ggcgatgtga
    12181 ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta tcagtgtttg
    12241 gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg atgattgttt
    12301 ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt atccgaaaag
    12361 cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt gtagatatcc
    12421 cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc gtttatacta
    12481 cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgcta gaacaagcgc
    12541 agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg gatattttgt
    12601 tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg ctttttgcta
    12661 atcgctttct ttgagtatct gtgtcaattt tgctatcctg agtatataga aagtgaggga
    12721 tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca aaagaaaaaa
    12781 tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa tcagaaacag
    12841 cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac cagcaggcag
    12901 aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca cctgatcgta
    12961 gtaaaacacc gctcccagaa agtgaccaca tttccgatga gcaaaaaatc gaggaacagc
    13021 gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa tcgagatgtg
    13081 acttgaagca gatgagaagg tgttcatctg cttcaag
//
LOCUS       DAFFSV010000081        13074 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFFSV010000081 DAFFSV010000000
VERSION     DAFFSV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.081"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(287..649)
                     /locus_tag="LR110_001977"
     CDS             complement(287..649)
                     /locus_tag="LR110_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822933.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="LR110_001978"
     CDS             complement(723..1103)
                     /locus_tag="LR110_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBL4822934.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="LR110_001979"
     CDS             1167..2360
                     /locus_tag="LR110_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL4822935.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="LR110_001980"
     CDS             complement(2437..3021)
                     /locus_tag="LR110_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL4822936.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="LR110_001981"
     CDS             3222..4220
                     /locus_tag="LR110_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL4822937.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="LR110_001982"
     CDS             4332..4841
                     /locus_tag="LR110_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBL4822938.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="LR110_001983"
     CDS             complement(4871..5530)
                     /locus_tag="LR110_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBL4822939.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="LR110_001984"
     CDS             complement(5532..6296)
                     /locus_tag="LR110_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBL4822940.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="LR110_001985"
     CDS             complement(6304..6981)
                     /locus_tag="LR110_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBL4822941.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="LR110_001986"
     CDS             complement(7018..8403)
                     /locus_tag="LR110_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL4822942.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="LR110_001987"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LR110_001987"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="LR110_001988"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="LR110_001988"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBL4822943.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="LR110_001989"
     CDS             9342..10199
                     /locus_tag="LR110_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4822944.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="LR110_001990"
     CDS             complement(10280..10615)
                     /locus_tag="LR110_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822945.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="LR110_001991"
     CDS             complement(10682..12052)
                     /locus_tag="LR110_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBL4822946.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="LR110_001992"
     CDS             complement(12130..12666)
                     /locus_tag="LR110_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4822947.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3947 a         2631 c         2159 g         4337 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attc
//
LOCUS       DAFFSV010000082        13008 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFFSV010000082 DAFFSV010000000
VERSION     DAFFSV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.082"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            45..1712
                     /gene="yfcC"
                     /locus_tag="LR110_001993"
     CDS             45..1712
                     /gene="yfcC"
                     /locus_tag="LR110_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBL4822948.1"
                     /translation="MIFGVVIFAWILTFVVPAGKFSTQDIEYKDANGETSTRTVLRQD
                     SFRYAYELDKSYVFDQLEELQDHPAEREKLDVPEKGLEKVIADGEKNLTQEKLDEISL
                     TDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGGFGFLNFVFEGLVSGDKYGSAVGIA
                     ALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLERLALPLLFFAFSFGGATFGMAEEV
                     IPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNATSWMSPFSVAVAQGIAGIPVLSGA
                     TFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLTYKSDDYFRSHIKKTSDENKKFMLG
                     HKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFFVMGLVAGIIAVLFKLDGMKVNDIA
                     SSFQSGAADLAGTAIVVGMAKGILLVLGGSDASVPSALNTILHGIGTALTGVPAVIGA
                     WAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADLVGVSRQVAVLAYQLGSGFMDAFTP
                     VSASLIGVLGVARIEWAKWARFQIKMQGFLFVLGTIFIMIAIAIGLQ"
     gene            1869..3053
                     /gene="iadA"
                     /locus_tag="LR110_001994"
     CDS             1869..3053
                     /gene="iadA"
                     /locus_tag="LR110_001994"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBL4822949.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3164..3367
                     /locus_tag="LR110_001995"
     CDS             3164..3367
                     /locus_tag="LR110_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL4822950.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3468..4130)
                     /locus_tag="LR110_001996"
     CDS             complement(3468..4130)
                     /locus_tag="LR110_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4822951.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4143..5645)
                     /locus_tag="LR110_001997"
     CDS             complement(4143..5645)
                     /locus_tag="LR110_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822952.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6193..7872
                     /locus_tag="LR110_001998"
     CDS             6193..7872
                     /locus_tag="LR110_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL4822953.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7999..8478
                     /locus_tag="LR110_001999"
     CDS             7999..8478
                     /locus_tag="LR110_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBL4822954.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8697..9377
                     /locus_tag="LR110_002000"
     CDS             8697..9377
                     /locus_tag="LR110_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBL4822955.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9385..10716
                     /locus_tag="LR110_002001"
     CDS             9385..10716
                     /locus_tag="LR110_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL4822956.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10731..11693
                     /locus_tag="LR110_002002"
     CDS             10731..11693
                     /locus_tag="LR110_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBL4822957.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11804..12142
                     /locus_tag="LR110_002003"
     CDS             11804..12142
                     /locus_tag="LR110_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBL4822958.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12221..12928
                     /locus_tag="LR110_002004"
     CDS             12221..12928
                     /locus_tag="LR110_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBL4822959.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4117 a         2307 c         2867 g         3717 t
ORIGIN      
        1 cgaaacgcga cttcactaaa ttatcgacac cccatacgta tgtcattatt tttggtgtag
       61 tcatctttgc atggatactg acttttgtcg ttccagcagg aaagttcagc acgcaagata
      121 ttgaatacaa agatgcaaat ggcgaaacaa gtactcgtac tgttttacgt caagattcct
      181 tccgttatgc ttacgagctg gataagtcgt atgtgtttga tcaattagaa gaactgcaag
      241 atcatccagc agaacgtgaa aaactggatg ttccggaaaa aggattggaa aaagtcattg
      301 ctgacggaga aaaaaatctt acgcaggaaa aactagatga aatctccttg acagatgatg
      361 tgctttacga tcagtacggc gaaaatatct atgatacttc taaaaaattg cataaaacag
      421 ccaaaatatg gggaacagat gattttggcg gctttggttt cttaaatttt gtttttgaag
      481 gactagtatc tggagataag tatggatctg ctgtagggat cgctgcattg atccttgttg
      541 ttggaggtgc ttttggtatt attatgcgta ccggagcgat cgacgcaggt atctatgcgt
      601 ttatcagtaa aactaaggga ctggagcgtt tagctttacc gctattattc tttgcttttt
      661 cattcggtgg cgctacattc gggatggcag aagaggtaat tccattctca atggtgatgg
      721 ttccatttgt cattgcgtta ggatatgact cgatcgtagc agtaaccgtg acttatgtcg
      781 catctcaagt cggaaatgcg acctcttgga tgagcccgtt tagcgtagcg gtggctcaag
      841 ggattgctgg aatccccgtg ttgtctggtg cgacattccg attgattatg tgggtagttg
      901 ttactgcatt agccgctggt tatatgatga tatatgcaga aaaaatccgt aaaaaaccag
      961 aaagttcgtt gacttacaaa tcagatgatt atttccgttc tcatatcaaa aagacatctg
     1021 atgaaaataa aaaattcatg cttgggcata aattgatctt attagagatg ttagttgtcc
     1081 ttgtctggat cgtttggggc gtgactcaaa aaggctatta cattccagaa atcgcttctc
     1141 aattctttgt gatgggatta gttgctggaa tcattgctgt tcttttcaaa ttagatggaa
     1201 tgaaagtcaa tgacatcgct tcttctttcc aatctggtgc agctgattta gctggtacag
     1261 caatcgtagt aggtatggcc aaagggattc ttcttgtctt gggaggatct gatgcatctg
     1321 ttccttcagc tttgaatacg attttgcatg ggatcgggac ggcgttgacc ggtgttccag
     1381 ctgtcattgg tgcttgggcg atgtacatct tccaaagttt gttcaacttg gttgttacat
     1441 caaattcagg acaagcagct ttgactatgc caatcatggc tccactggca gatttagtag
     1501 gtgtatccag acaagtagca gttcttgctt atcagcttgg ttcaggtttt atggacgctt
     1561 ttacacccgt atcagccagc ttgattggtg tgctaggtgt tgcacggatc gaatgggcaa
     1621 aatgggcaag attccaaatc aagatgcaag gattcctgtt tgttttagga actattttca
     1681 tcatgattgc cattgcaata ggtctgcagt agtactggag acctgtaaat aaaaaagtaa
     1741 tccaaacagt agataaggac tccaagaacc gtcattttac ggtaaaatca aaggagtaaa
     1801 gatcctatcg tttggattat tttttaagat cctatcatca gttatactta aagaaagagg
     1861 aaaaatgtat gaaaatcatt cgacaaattg aagtgtttgc accagattac ttaggaaaaa
     1921 tggatgtact gattgccggt gggaagattt tggctgtaga agaacagcta gaaggcggct
     1981 acgaaggagt ggaagtagaa gaactttcag gtgagggaaa agtactaacc ccgggtttca
     2041 ttgactgtca tttccatatt ttaggtggag gaggagaagg cggttatcaa aatcgaacgc
     2101 cagaagtcac attgagccaa ttgactacag ctggtgtaac gacagttgtc ggttgtttgg
     2161 gaacagacgg agaaggtaga gatatgactg cgttgatcag caaagcgaaa ggtctggaag
     2221 cagaaggaat ctccacttat gtatacgaag gttcgtaccg attgccggta aaaacagtaa
     2281 cagattctat catcaaagac tttttgacaa ttgataaaat tatcggaatc ggagaaatcg
     2341 cagtatccga tcaccgttct tctcagccaa gttttgaaga gttcgctcgt gcagtagcag
     2401 atgctcgtgt aggaggcatg ttatccggaa aagctgggat catcaatgtc cacttaggcg
     2461 gaggaaaaag aaagctagag ctattgacac gaatcgtaga agaaacagaa attccgatca
     2521 cacaattttt gcctactcat gcaaatcgca caccagaatt atttgaagct tgcgtggcat
     2581 ttgccaaacg tggtggcacg attgacttca ctgctagcga agatcctgat ttttgggaga
     2641 agacagatgg tgaagttcgc ttcagcaaag cactgaaacg attgatcgag gaagatgttt
     2701 ctttagataa tttcacgatg acctcagatg gacaaggaag cttgccttat tttgatgaaa
     2761 acaatcattt cctagggtta ggagtaggaa gtgccaaggc attgctagta gggatcaaag
     2821 aagctgtgca aaaagaaagt atccctttgg aaatcgcctt acgagctata accagtaatc
     2881 cagcacgtat attgaaattg gataaaaaag gaaaaatcga aattggtgct gatgcagatc
     2941 tgtgtatctt ggataaagaa acgcttgata ttgatacagt tatcgctaaa ggagaaataa
     3001 tggtccaaga aaaagaagtc aaagtatggg ggactttcga aaaaagtttt taatcaacag
     3061 ctctaactat cttttttaga aaacgagcgt tctgtttgtc tgtatctgtt gttgatgtta
     3121 ccctgaaggt agatcaataa acaaactaag aggaggaagc acaatggcag atttaaaagg
     3181 acgttctaaa gatatgaaag ataaagtagt cggtgaagga aaagaagcat acggcaaagc
     3241 aacagacgac aagagcaaag aaacagaagg gaaagcacaa tcactaggtg ctgacatcaa
     3301 agaaaaagct cgaaatttag gcgacgaagt caaagacaag ttttctcgaa aagacgaaaa
     3361 aaaataaaaa ccacttatta ctaattgaat caagcaaata aaaaatccga accatatctg
     3421 cgagacagat atggttcgga ttttttattt gatcattatc ctgtttttta aacttcgatt
     3481 ttttgaaatt ttttattttt tagttcaaag acttcatcac agctttttgc tacttcaatc
     3541 tcatgagtca cgatgataat acagcgattt tgttcatggg cgatttcttg gaaaagtttg
     3601 acgatatctt gtgaagttgt ttcatctaga tttccagtag gctcatccgc tacgattagt
     3661 tgatgttcac agcagatagc acgaacgata gcgactcgct gctgctgacc tcccgaaagt
     3721 ttggtcacct gtttttttgc taatatttca tcaattccta cacgagccag tcgatctaaa
     3781 gcaacttgtc gataattctc ttgcttggtc ttagtgattt ccattgctgc tatgacgttg
     3841 tctagagccg ataaataagg aagcaagttg taagcttgga aaataatcga gacatcattt
     3901 ttacgataac ttcttaaccc gattttgttc agtgaacgat cttgatagta tacctttcct
     3961 tcttttggtg catctagtcc agaaataagc gaaagaaaag tcgtttttcc agaaccactt
     4021 gctcctaaaa tagcgtacat ttttccagct tcgaaattca gttctacatc tttataaagc
     4081 gcatcttgcg gatcttgata ccaaaatccc aattgttctg tccgtaacat caaattcctc
     4141 ctttagttta ataggatttt tcttgggttt aatcttaata tccctataga agctaaaaag
     4201 acagaaaaca gactgattcc aaatgctact ccggctaata gtcccaattg tgcaggtttg
     4261 acattgattt ccaattcgct gacttgatca gaaacagcaa atggattatt cgtggttcct
     4321 tgttgaccat cgcgttgacc gccttgttct tgtctgttac ctggcatttg tccaccgcca
     4381 ggagcttgct ggttttgtgt cgtggtttct gtctgttggt caagtagctg ctgtcccacg
     4441 gcattcgcga cgacgtttcc gctaagcgaa gcaactccta atgcaagaat catacaaata
     4501 gccacttcgg taaacaattg gagaatgatt ttacttctgg attctcctaa agatagcaag
     4561 acgcctaact catgccgacg ctcccgaatg gtaatcataa tgatcagagt aagtatgata
     4621 ataccggcta cagccactag taaaacaaca tttttagaaa aacttgccac gttgtttaaa
     4681 ggttccaaca ttgactgata catggaatca tttgattgta agctaaatgt atctgtgtcg
     4741 atcagcttct cagcttcttt gagaaactca tccattttat ctggatcaga tagagtgtaa
     4801 atagcagaat ctatcgtgtt atcgtcagat gtcccattta gttcatttgc aaaagtgtaa
     4861 gaagtgtaaa gtgtattcga cggattcatg aagttgaatt tcatacccat actgccgctt
     4921 gtttcgctgg attcataaat tcctttgatc gtcatttcat aagtggtatc ttcatcttca
     4981 gtactcgtga ttgtgaaagt atctccgact gccaggtcat ttgtttctgc tagagtagaa
     5041 tcaatgacta cattgtttgt gcctttgtcg ctgtcatcta ttccttcacc atccgtgatt
     5101 tttgctgttc catcagaaaa attactattt tgtgcagttg ctgacacacc actgatttga
     5161 aagtctgctt gagtcatttg cggattcccg cccattcctc cagcaggttc accgcctgca
     5221 tcagtagttt ctgaactgtc ttcacttgaa tcagaactag tgatggcagt tattccatct
     5281 tttgccatag ctgaggcgga actttcaaag gaataactct ttacattatc taattcagct
     5341 attttttccg cgtccgttaa ggaaactggt gtaagagaaa aactgcctgg atctggacgt
     5401 tcttcagtgg tagaaccgct tgaagaagtt tgttcctgtt ttttaaatga agcttctcga
     5461 ttggcactta aagtcactgt tgctccaact tctttttgtg cttgattcgc tgcttcttct
     5521 gcagcgcttc gaatcgtgag gccagctagc acaaaaataa gaatggctgt gaacacggca
     5581 aacaataaca atgaacgtcc ccattttact tttgtacttt tccatgcacg tttcataaaa
     5641 ttcatatttt gcatccctcc tagtagatgc atttgataat agacaaccta tcttaaagct
     5701 agcttaaatc ttatgctgtt tgcacaccat caacatgagg ttctgtatat ataaaaagtc
     5761 actatagtta ttgcaaaaaa tgtggtaaaa tgaaaaaagt atgtttcaaa ccgacgaaga
     5821 agaaatgaaa gaggaatagc aaaagaaatc ctagatgcta cagagggatt tttacgtgca
     5881 tagttgttct aacaattgct gatactagcg atttagttta tttgttgtat aataatgggg
     5941 aacagctgaa tacttatata agtattctgg ctatagcgtt tcgcaaaaga tccattttgt
     6001 taatgaagta tatccaaaat aaaatgccca tccaactgtc agaacgcgga tgtgctggta
     6061 attttcctgc tgattctcca taagttgaga aatagtgatt ttgcatatgc ttcattggaa
     6121 tggctcatat atttagataa agaagaaaat cgacatcttt aaacttggat gtcataagaa
     6181 gaggtgaagt ttgtgagtac cattaaaatt atcccattag gcggcgttcg tgaaaatgga
     6241 aaaaacatgt atgtcgcaga agtgggcgag gatattttta ttttagattg tgggctaaaa
     6301 tatcctgaaa atgagttgtt ggggatcgac gtcgtaatcc ctgattttac gtatttagaa
     6361 gaaaacattg atcgtgtagc tggtgtcttt ttaacacatg gacatgcaga tgcgatcggg
     6421 gcacttcctt acttgttgtc aaaaatttct gtaccagtat tcgggacaga gttgacgatc
     6481 gagttagcaa aattgaatgt cagtcgtaat gaagcagcca aaggatttaa ggatttccat
     6541 gtggtagatg agcatacaga aatagacttt ggtgaaacag tagtgagctt tttccgtacc
     6601 actcatacga taccagattc agtggggatc aatctgaaaa caaaagaagg tagtatcgtt
     6661 tatacgggag attttaaatt tgaccaaacg gctatcccaa tgtatcaaac agattatgca
     6721 cgattggcgg aaatcggcaa agaaggagtc ttagccttac taagtgaatc ggcaaatgca
     6781 gaaaatcctc agcctgtagc ttctgaagcc caaatagcag atgaagtatt tgatacgatc
     6841 aaatattggg aagggcgtat catcgtcgct tgtgtagcaa gtaatttgca acgagtgcaa
     6901 caagtattgg atgcagcaga aaaagcacaa cgaaaagttg tcttgactgg tcaagatttc
     6961 gaacgaatta ttcgcacagc gatgcgttta gaaaaacttc aattaccaag tgaagatctg
     7021 cttgttacgt tgaaagacat gaaaaactat gatccagaag aactgatcat tttagaaact
     7081 ggccgaatgg gtgaacccat caagtctctg caaaaaatgg caaacggtac acaccgtaat
     7141 gtcaagattg aagaaggaga tttggtttat atcactacta cgccaagcat cgcgatggaa
     7201 accatcgtgg cgaaaacgga agatatcatt tatcgagctg gtggaacagt gaagttgatc
     7261 tctgagaata tgcgcgtttc tggccatgcc aatcctaatg acttgcaatt aatgatcaat
     7321 ttgatgaaac caacatattt tgtgccagtt caaggagagt atcgtgaatt agctgctcat
     7381 gcagatttgg ctcatgcagt agggatgcca tacaaaaata tctatattac tggacgcgga
     7441 gacgtgctgg aatataagaa caagcgtatg agtgttgcag gaactacaag tgcagaaaat
     7501 gtaatgattg atggattagg cataggtgat atcggaaaca ttgttttacg tgatcgaaaa
     7561 atcttgtcag aagacgggat tttcgtagcg gttgtcacta tcagtcggcg agaaaagaaa
     7621 atcatttcaa aacctcaaat tacttcacgg ggattcgttt acgtaaaagc tagccgtgat
     7681 ctgatgaaag aaagcagcaa tatgattgaa acaatcgttg agaaacattt agaaagcaat
     7741 gattttgagt ggagcaaatt aaagcaagac atccgagatc aattaggccg ttacttattt
     7801 gaacaaacaa aacgccgccc agtgatcttg ccagtcatta tggaagctac tcaacgtcga
     7861 aataaaaaat aaacaaaaag agtaagatga acctctctat agagtgctcg tcttgctctt
     7921 tttttgcgaa aaacaaataa tttttgaata cagcatccct cgctttataa taaacgatag
     7981 aataaaggga gtgaatcaat gatgagtcaa acaaaagtcg aacgttattt agaagaacaa
     8041 aagatcagct atcaaccact tgattttagt catgtggtag aagaatcttt cacagaagaa
     8101 ctacaaaaaa gagggattga tccagctttg atatgtaaaa ctctcgtagt gaaaggaaac
     8161 aaaactgggg tgatcatcgc attgattcct ttgaacgatc acttagatta caaaaaaacg
     8221 agacaagtga cgaaagatcg taaagttggt tttcctggaa tggattttgt attaatgcat
     8281 acaggatatc cccacggagc aaacacccct attggaataa aactcgcaca tcctgattat
     8341 tgttttttag cagacagttc tttaaaagaa aaagaagaag taattgtgtc aagcggtgaa
     8401 atcgggagaa gccttcggat aaaagtagcc gatttagaaa agatcgtcca tcctatttac
     8461 gctgatttaa ttagttaagg gacgagaaga gaagctaagc agagcagaga cctcaaactt
     8521 gagattatat ctgcttggta cttggtgcat cctgtattat ctgttacaat tgctttgtac
     8581 atactgtact atcaatccaa tgaaaatata tttagcagct atattttttc attgatgtgc
     8641 aagtgatgaa atcgttttta ataaaaaaga tacagagaaa acgcgaggtg taaaatatga
     8701 aaattacgta tcatggacat tcctgtttaa caattgaaat ggaaaatggc caacgcgtga
     8761 tgattgaccc gtttattaca ggaaatccat tgacagactt aactgcagaa gaagtaaaga
     8821 cagattggtt gctgattact catggtcata gtgaccacat aggagatatg attcctattg
     8881 caaaagaaaa tcaagccaca attatcagta ttgttgagat tgctgattat gcgaacagca
     8941 gaggagtaga ttcattcggt atgaatatcg gtggaaaaca tgcgtttcca ttcggcgtgg
     9001 taaaattcgt acatgcacag catagttcaa gctatgaagt agacggtatc gttcagtata
     9061 tgggcgagcc tagcggaatt attattcaag cagaaggaaa aacgatttat catgcagggg
     9121 atactgctta tttcagtgat ctaggattgc ttgcagaaga attcgatatt gatgtcgctt
     9181 tcttgccgat tggcgataac tatacgatgg ggccagaaga tgcagcaaag gctgcgcaaa
     9241 caattcgtgc caagaaagta gttccaatcc attacaatac tttccctgtc attgaacaag
     9301 atcccgaatc ttttgtagag ttactggaag atagtgaagg aaaaataatg agcgtctcgg
     9361 aaacgctgga agtgtaggca gaaaatggcg acaaaacatg atttgatttt agaatatatt
     9421 gaaagtttac ctgttggcaa tcgaatttct gtaagaagca tcgcaaaaga attgaatgtc
     9481 agtgaaggaa cagcgtaccg tgcaatcaaa gatgcagaaa atgtcggact agtttcaaca
     9541 attcaacgag tagggaccat ccgtatcgaa cgcaaattaa aaaaacatat tgaacgtttg
     9601 acatttggcg aggtcgtcag gatcattgaa ggggacgttt taggcggggc ggcaggatta
     9661 gacaaggtct tgaataaatt cgtcatcggc gcgatgacta aacatgccat ggaacgctat
     9721 attacgccag gatcactaat gatcgtcggc aaccgggcgg aagtccaaaa attagcattg
     9781 gaagatggcg ccgcagtatt gatcacagga ggattcaata cgactgctga gattgcaaag
     9841 ctagctgatg atcttgaaat gccgatctta cgcacctctt atgatacatt tacagtcgca
     9901 accatgatca accgagcgtt aagcgaccaa ttgatcaaaa aagatatcat gcttgtcagt
     9961 gatatctata tgccagctga aaagactcat tatttgcagc agacagatac gatttctgat
    10021 tatcagcgtt tgtcagaaga aacgcagcat tcgcgttttc cagtcgtcaa ccgccatcat
    10081 cgtttgatgg ggattgttac ggcaaaagat gttttaggga aatcgccaaa ccagatcatc
    10141 gatcgggtga tgacgaaaga accaatcagt gtaaagaaaa caatgagtat tgcctctgtt
    10201 agccatcaaa tgatctggga tgggctagaa gtcatgccgg tcgtttccga tgacttgaca
    10261 ttggaaggct tgattacccg gcaagatgta atgaaagcga tgcagcttgt ccagcgacaa
    10321 ccgcagattg cagatacgat ttcggatcag atatctggaa atatccatac gattgatact
    10381 gaccgtgaag gaaatcgttt ggaaaaccct aagttcaaat tcgtagtagc accacaaatg
    10441 gtcaatggcg ttggaaccat ttcttttggc gtacttagcg aaatcatttc tgatgtcgca
    10501 caaaaaacaa tggtgatgaa ccaaaaacga aatatcttga ttgaacaagt gaacctacat
    10561 tatttgcggc tgatccaatt ggaaagcgaa ttagacattc gtccacgtgt attggaaatc
    10621 ggcagacgtt ccgctaaact ggatatcgaa gtgtttattg aaaacaccat cgtttccaaa
    10681 gcaatcgttg tttgtcaagt aatggaacgt tcttagaaag caggagtgaa atgacagtac
    10741 agcaagaaat agtagataca ataaaaaaat acgataaaat cattatccac aggcatttgc
    10801 gccctgatcc tgatgcatta ggatcacaag taggattagc cgaactatta cgggaaagtt
    10861 tcccagaaaa agaaatacgt caagtaggtg aaacgattga agggcttcgc tttttagcag
    10921 aaatgcaaga agttgaagat gtcttctacc aaggagcgct agtcatcgtg actgatacag
    10981 ctaattcacc cagaatcagt gatcaaagat atcagttggc agaaaaactg atcaaaatcg
    11041 accatcatcc caatgatgac ccttatggag atcttgtgtg ggtaaataca aaagcaagta
    11101 gctgtagtga gatgattgca gaatttgctt tgatgtttcc tgaatcttta aagatgaacg
    11161 ttaaggctgc acggttactt tatgcaggaa tcgtcggaga tacagggcgc tttttgtatc
    11221 cctccacgac gtcccgtaca ttagagattg cttcgatatt gagaaattat ccatttgatg
    11281 caagtgcttt gaacagagag attgaacaaa tgccgatgaa agtcgctaaa ctttccggtt
    11341 atctttatca aaatatccag gtggatgaaa atggtgcagg aagaataatc cttccgcaag
    11401 aaattcttga taaatatgga atcgatgatt cagagacagc ttcgatcgtt tcacttcctg
    11461 gagtgattga cgaagtgctg gcttggggga tttttgttca gcagccttca gggtattacc
    11521 gcgtacgttt acgttctaag gggccgatca tcaatgaatt ggctaaacgc catcatggag
    11581 gaggccatcc attagcaagt ggtgcgaatg caaaagattt agcagaagta gaggaaatct
    11641 ataaagagat ccaagatata tgccaatcgt ataagagcag aaaaacagaa tgatacttcg
    11701 ttcgaaaacg tgccttttgt agtaaaaatc acaaatgtat cttttcaaga tcttcttttt
    11761 ccgttacaat agtgctcact aaagatttta aaggagaaag aagatgacat taccaaattg
    11821 cccagaatgt ggctcagagt acacatatga agatcgaggc ttgttgattt gcccagaatg
    11881 cgggaacgaa tggaacaaag aagaacaaga aactgtcata gaagacggat tagtggtatt
    11941 agatgcaaat ggaaaccgtc tgttagaggg cgatagcgtg acagtgatta aagacttgaa
    12001 agtaaaaggt gcgtcaggtg ccattaaaca aggaacaaaa gtcaagaaca tccgtttagt
    12061 agaaggcgat cataatatcg attgcaaggt agaaggattt ggaccgatga aactgaaatc
    12121 ggaatttgtg aaaaagaatt aaagaatatt caaggtcttt tcacattctt ttcctactac
    12181 taatgataaa ataaagtaaa cgaaaaggag tgaaacgaaa atgtatggtt atggaatgta
    12241 tggtttttta gatccgacat ttcttttggt cattcttgga ttagttattt ccggaattgc
    12301 gtcagcgtac gtcaatagta ctttccgaaa atacgatcaa gtgagaagca aaaacaatgt
    12361 gactggtaca caagcagcac aatatatatt acaaagtcaa cagataaata atgtcggtgt
    12421 tcaacaaatt gccggagatc tcacagataa ctacaattca ggtaataaga tgttgagttt
    12481 gtctcaagca actgcacagt ctacttcagt tgctgcgata ggtgtagcag ctcatgaatg
    12541 cggacatgcg gtacaagatg cttcgggtta tgtcccattg aagattcgtg cagcacttgt
    12601 tccagcagct aactttggtt cgatgatttc gttccccttg attatggtag gtgtattgct
    12661 tagctggaac tctacgttaa tcaatatcgg gattttcgct ttttctttag cattactttt
    12721 ccaattggtg acattaccag tcgaatttaa cgcgtctcgg cgagcaatcc aaattttgag
    12781 tgaaggcgga ctactgacgg aagaagaagt accgatggca agacacgtat tatttgcagc
    12841 agcactgact tatgttgcag cagcgctttc gacattcctg cagctgttgc gcttgatctt
    12901 attgttcggc ggcaaccgaa gagattaaaa taaagaagtt gtgaaatcag cgctttgatc
    12961 tgagctaata ccgagagtta gctgattgaa taccatttat ttggaata
//
LOCUS       DAFFSV010000083        12969 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFFSV010000083 DAFFSV010000000
VERSION     DAFFSV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.083"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(185..1633)
                     /gene="cls"
                     /locus_tag="LR110_002005"
     CDS             complement(185..1633)
                     /gene="cls"
                     /locus_tag="LR110_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL4822960.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1785..2678)
                     /locus_tag="LR110_002006"
     CDS             complement(1785..2678)
                     /locus_tag="LR110_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4822961.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2662..3348)
                     /gene="ftsE"
                     /locus_tag="LR110_002007"
     CDS             complement(2662..3348)
                     /gene="ftsE"
                     /locus_tag="LR110_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBL4822962.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3453..4554)
                     /gene="prfB"
                     /locus_tag="LR110_002008"
     CDS             complement(join(3453..4481,4483..4554))
                     /gene="prfB"
                     /locus_tag="LR110_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBL4822963.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4680..7214)
                     /gene="secA"
                     /locus_tag="LR110_002009"
     CDS             complement(4680..7214)
                     /gene="secA"
                     /locus_tag="LR110_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBL4822964.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7436..7987)
                     /gene="raiA"
                     /locus_tag="LR110_002010"
     CDS             complement(7436..7987)
                     /gene="raiA"
                     /locus_tag="LR110_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBL4822965.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8115..8834)
                     /locus_tag="LR110_002011"
     CDS             complement(8115..8834)
                     /locus_tag="LR110_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBL4822966.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(8831..10144)
                     /locus_tag="LR110_002012"
     CDS             complement(8831..10144)
                     /locus_tag="LR110_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL4822967.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(10225..10545)
                     /locus_tag="LR110_002013"
     CDS             complement(10225..10545)
                     /locus_tag="LR110_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4822968.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(10653..10940)
                     /locus_tag="LR110_002014"
     CDS             complement(10653..10940)
                     /locus_tag="LR110_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822969.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            11067..11711
                     /locus_tag="LR110_002015"
     CDS             11067..11711
                     /locus_tag="LR110_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBL4822970.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(11770..12477)
                     /locus_tag="LR110_002016"
     CDS             complement(11770..12477)
                     /locus_tag="LR110_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4822971.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(12697..12867)
                     /locus_tag="LR110_002017"
     CDS             complement(12697..12867)
                     /locus_tag="LR110_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822972.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3587 a         2813 c         2251 g         4318 t
ORIGIN      
        1 gtgtctcttt taatagcata tattagctta tttcttgctt tccccaaaaa acaagagata
       61 ccccaaagtt aatttcgctc atgcgagacg ctttctccat ttctgacgta ctctcagaaa
      121 tggccccgtt agaaaaggac acgagatctc catctgttga tggttctcat gtccttttta
      181 tgttttaaag gagcggcgaa atcaatcttg ctaatccttc tttcatcttg atgattattc
      241 ctcggttttc atacatttct tttgtgagat gcgtagaaat tttcgagtct tcaaaaaaag
      301 cttttcttac acgtgaagcc attcgttcat cataaaccag tgtattgacc tcaaagtcca
      361 agcgaaatga acgaacatcg atatttgctg agccaactga aacaatgcca ccatcgataa
      421 tcatcgtctt tgcatgaata aatccattct catatgtttc gatccttgct ccatactcga
      481 tcagctctgc agcgaaagaa tacgttgccc agtaaacgag aagatgatct ggtttgttag
      541 gtatttggat atgaactttt acgcctgaaa gaagagccaa cttcagtgct tcatgaatcg
      601 atccatccgg tatgtaataa ggcgtttgga tcaatatctc ccgtttggct aacgaaatca
      661 ttttcaaata agtcattttg atttgttcat gttcggtatc tggtccgctt gtcacgattt
      721 gaacagctat atcaccagta gtgtgaatgg acgggaaaaa tttgggagaa tagcctactt
      781 cttttgtatg ctgagaattc cagtccatca aaaaacggtt ttgcaaggta tacaccgctt
      841 ctccgtaaat acgcaaatga ttgtctcgcc aataaccaaa tttctttacc aatcctagat
      901 attcatttcc aacattgaac ccacctgtat atccgatttt cccatcaata actacgattt
      961 tacgatgatt gcgataattg atccgaggat taatatacgg aacaaataga ggaaagaaaa
     1021 atgcgacttc tccgcctgct tttttcaaat tctgaaaaaa gctggaagaa acttgtgtgg
     1081 aaccccatgc atcaagaagg actctgactt ttacccctcg tcttgccgcc tctgtcaatg
     1141 catctcgaac ttcttcacct aatgcatcac tgcgataaat ataatactgg aagtggatat
     1201 ggtcttctgc ctgcttgata tcttcaatca gcgcatcgaa tttttcacga ccatctgtaa
     1261 ataaagtgat ttcattttta gtcgtataga gcgtactttc aaataccgtg agcatataga
     1321 tcaattgttt cacatctact tgtccagtag gcggatgagg ataaagccct cgcatcaatg
     1381 cctgtttgtc ttcttcaatt tccaggttct taccgatttt cccttggatc ttcaaatcaa
     1441 agattttctc tttggaaatc cctcttccta aaaaaatata taaaataaat cctacaactg
     1501 gaatgaacat caaaacgagc agccatgccc atgtttgagc agtttccttt ctttctcgaa
     1561 aaacgatgat aaaggaaagc aaaatattca taatcaaaag gatcgtaaaa aaattgtcaa
     1621 cgataatttt cacggcttca cctcttcttc taccaactta agaataccca agttgttttt
     1681 taaagtaaag ggaaaaaagc cgcttccttc tattctccta gccaataaaa aaagaaagct
     1741 ggaacgaaac tgtccaactt tctcatttta tttatattgt ggtattagaa tttcaaaaaa
     1801 cgtctcatag aaatgatcga gccaagtgaa ccaatgatca caccaatgcc gaccattaaa
     1861 agattgattt tccaaacgat attttgtgga cgaatcaatg aataatggga acgtaataat
     1921 gatggattga tcaattggaa aaaccatgca taaccaaatg tgatgatcaa aatcggaaga
     1981 atcgcaccga tcagaccgat ccagccacct tcaaggaaaa acggccagcg aatatatcca
     2041 ttttttgcac cgactaaacg catgatttga atttcacgct gtctagacaa aatcgtgatc
     2101 cgaatcgtgt tcgaaatgag aaacatcgct acgaacagca gcaatgccgc tgcaactaat
     2161 ccccatgtcc gaactgcacc agcgatttgg aaaatccgat ctgaggaagc tccaccgtaa
     2221 tcagaacgag aaacattctt caaatctgct gcttcttctg ctacttgttt tgtataagaa
     2281 ggatcagtag cacttactac atacacatca tataacgggt tattgtcacc ttcaaacaat
     2341 ttccaggaat cacccatttc gttttggatt tttttcaatt cctgttcttt gcttgaaaat
     2401 tcaacttttt taacatgatc taagtcattc aattcttttt ctagcttttt catctcttcg
     2461 tcttttgtac cgatatctac gaatacagat acatcaacgt ttccttctat atcctgagct
     2521 aatttagttg cattcatgat aactgccatg aatacaccga ctaaagtcaa cgtgatcgtt
     2581 accgcactga ctgaagcaac tgtcatccag ccgttacgtt tcaaactctt gatactttct
     2641 aataaatggc ggaaaaaggt tctaatcatc gtatccatat tccccttccg cttgatctcg
     2701 aataatgcgg ccgttttcga tagcaattac tcggtgccga atcgtattta cgatcgtact
     2761 gttatgtgta gccatcacga cagtagttcc ttgggcattg atccgatcta ataatttcat
     2821 gatttcccat gagttttctg gatccagatt tcctgtcggt tcatccgcga taagtacttt
     2881 aggcgtattc acgattgccc gagcaataga gacacgttgc tgttccccac cggataattc
     2941 gctaggaaag actcgaactt tgtgtttcaa accaacaagg tccagcactt ccattactcg
     3001 ttttttgata tctcgaggtt ttctgccgat aacttgcata gcgtacgcaa cattttcata
     3061 tactgttttt cttggtaata atttatagtc ttggaacacc acgccgattt ctctgcgtag
     3121 ataaggaact tcgcggtttt tgattgctat tagatcatga cctgcgacgt tcaaagttcc
     3181 ttttgaagct ttttcttctc gatacatcaa tttgatgaat gtagatttac cggctccaga
     3241 cggaccaacc acatagacaa actctccttg ttcgatgaca atcgagagat tgcggatagc
     3301 ggtcgtacca tttgagtact tcttcattac atccttcatt tcaatcatgg cgttctctcc
     3361 atatcttttt cagtctttag ctattatagc atggcaatgt ttcaacatgc ttgcaaaatc
     3421 ttacaatttt atttcaattt aacaatgagt tcttattgta gatgcatttt taaatagccg
     3481 tcaataaata aatcaaggtc accgtccata actgcttgga catttcctgt ctctacgttt
     3541 gtccgatggt cttttaccat agaataggga tggaaaacat aagaacggat ctgtgaaccc
     3601 cagccgattt ctttttgttc tcctcgaagt gcagctgctt cttgttcttt cttttccact
     3661 tctaattgat aaagcttagc ttttaacatc cccatcgctt gctcacggtt tttcaattgt
     3721 gagcgttgtg cctgactagc tactactgtt cctgtaggca agtgagtgat ccgtacagca
     3781 gattctgttt tattgatgtg ctgaccacct gcaccacttg ctcggtacgt atcgattttc
     3841 aaatcatctg gattgatttc tatatcgatc gtttcatcaa gctctggcat cacatctacc
     3901 gaacaaaaag aagtatgacg tcgtttcgct gagtcaaaag gcgaaatccg tacaaggcga
     3961 tgaacacctt tttctgattt tagataacca aaagcgttat gtcctttaat cagcagggtc
     4021 acactcttga tccctgcttc atcacctgct tgataatcta gtgtttctac ttgatagcca
     4081 tgctgctctg cccaacgtgt atacatacgt aaaagcatgc tgccccagtc ttgggactcc
     4141 gttccgccag cccctggatg caattcgacg atcgcattgt ttcgatcata aggtccgtct
     4201 aacagcatag ataattcata cgtcttcatt ttttcatcta atgcattgat acgtgtttct
     4261 gcttcttctt gcattgctgc atcaggttct tcttgcagca tctccaccat caattcgatt
     4321 tcttctagtt cttcggcaag agaatggaat tggtcatagg tttctttatt agcgttattt
     4381 tcgctgatca ctttttgtgc ggcttcgctg tcatcccaaa aaccaggttc agccattttt
     4441 gcttctgctt ccgcgatgtc ttcttccagc tgatctaagt caaagagacc tcctgaaact
     4501 tgtgaccttt tcctgcattt ctgtcaggag atttcgaatt tcggatattt ccatgttttt
     4561 tccttctttc actatacttt tttcattata tctagtctaa cagaatcagc aaataacaga
     4621 aagaaggtga gacgaattct taaaaaaaga atttgtctca ccaatcctgc tgtcagaaat
     4681 tagtttgcat tttttccatg acagttctta tattttttcc cgcttccaca aggacaagga
     4741 tcatttcttc cgaccttgtt cacatggatt ggctgttttt tcgcgctagt attactattt
     4801 tctttggcat ctccgtcttc tgagtgagaa gctgttcctt gagaaacctg ttcacgctgt
     4861 acgttttgac ggatctcagc tttcataaag attcgagtga cttcatactc gatggcgcct
     4921 accatatctt caaacatctt atagccttct gtttgatatt cgactaatgg attgttctgt
     4981 ccataagcac gtaagccgat tgactgacgt aattgatcca ttgcatcaat atggtctgtc
     5041 catttagagt ccacaacacg caagatcacg actttttcaa attccaacag ttgctctgga
     5101 ccatttagtt gagccgcttt ggtattaaat acttcttgtg ctcgttcgtt tagatactct
     5161 ttgatctcct gagatgtttt tccttcgata tctgcaagag taatactatc ttcatggaca
     5221 agagtactgc cggcaaaatc gatgatactt tccaaattcc aattttcttt ctctaattgt
     5281 gtatgcgcat ctactacacg actgattgtc cgtttgacca tgttcattag tggcttggat
     5341 aagctttttt cttccatgat tacttcttgt cgctgtttat agattacttc acgttgttca
     5401 cgcatcacat catcgtattg taagacgttt ttacgtgtgt catagttatt tccttctaca
     5461 cgtttttgtg cagattctac ttgtttcgtc agcatcttgc tttggataac agcatcttcc
     5521 tcaccgatct tcatccgatc taagaaagct ttgattcgtt ctgaaccgaa tcgtttcatc
     5581 aaatcatctt ccaatgacaa gtagaattgt gacataccag ggtctccttg acgtcctgca
     5641 cgtcctcgaa gctggttatc gatacgacga gattcatgtc gttctgtccc gataactgct
     5701 agaccaccaa gttctcttac cccaagtcct aacttgatat ctgttccccg accagccatg
     5761 ttggtagcaa tcgtcacagc acctttttga ccagcattca tgatgatctc tgcttcttta
     5821 aagtgatttt tcgcattcag cacttcatga ggaatacgtt cttgattcag catatctgat
     5881 aataactcag atgtttcaac cgcaacagtt ccgactaaca caggttgacc tttgcggtag
     5941 cgatctttga tatcttgcac gacagcgtgg aacttgcttt gtaatgttgg gtaaagtaaa
     6001 tctggacgat catcacggat aaccggacga tttgtcggaa tctggtatac ttggatgtta
     6061 taaatttctc tgaattcttc ttcttctgtt ttagctgtac cagtcattcc cgcaagtttc
     6121 ttgtacatac ggaaatagtt ctggaaagtg atcgtcgcca tggttttcgt ttcgtcttcg
     6181 atctctacgc cttctttagc ttcgattgct tggtgaagtc catctgaata acggcgtcca
     6241 tccatgatcc gaccagtgaa ctggtcaaca atcagtactt ttccgtcttg gacaacataa
     6301 tcgatatcta aaagcatgat atagttggct ctcaatgctt gatccaagtg atgggtcaat
     6361 gcagtatttt caatgtcgta taagttatcc aaaccgaatg tttcttctgc tttttcgatc
     6421 cctgcttcgg ttaaactgat tgtttttgat tgtacatcga ttttataatc ttcttcttct
     6481 ttcaaacgtt tcacgaagtt gtctgctctt gtatataaag ctgtggattt ttctgcttgc
     6541 cctgaaatga tcagcggcgt tctcgcttca tcaatcaaga ttgagtcaac ttcatcgaca
     6601 atggcataat tcagcggacg ttgtaccatt tgatggcggt aaacgaccat attgtctctc
     6661 aagtagtcaa atcctaattc attattcgta ctataagtga tatcacaatt ataagcctcg
     6721 cgtttttcat ccgaagattt tgagttgata ttcaacccca ctgttaatcc taagaaatta
     6781 taaagttcgc ccatttccgt cgagtcacgg gttgctaaat attcattgac ggtaactacg
     6841 tgtacccctt cgccagtcag agcatttaga tatactggca tcgttgcggt caaggtttta
     6901 ccttcaccag tacgcatttc agggatattt ccatcgtgaa gcacgattcc acccatcaat
     6961 tgtacgtggt atggatataa ccccaataca cgttttgctg cttctcggac gactgcaaaa
     7021 gcttccggaa gtaattgatc taatgtttct cctttttggt atctgccctt gaattcagca
     7081 gtttttgcct gtaattgttc atcagttaat gctgccattt ggtcagcgta tgcttcaact
     7141 ttttttgcaa tgctatctaa acgttttagt tctttcttat cgttttcgat aagtgaacga
     7201 atcaaattgg ccatgcgttg tgtaatctcc ttttccttct gtgtattatc cagactatgt
     7261 agactttact tttcatttta tcattagtta tagataattg aaatatctta agcgtattaa
     7321 agtaagaact tcataattta ttcatatttc atctaaaaga ggaggaaact caaaaaaact
     7381 tgcggcagct gtcagtagct gctgcaagtt ttcgttgtta tttaagtaag gctgatcagt
     7441 ctgtttcaat cagtccgtat tttccatctt tacggcggta aacgatactt gtaccatttg
     7501 tttctgcatc ttcaaaaata aagaagttat gtcccagcat gttcatttgt aagactgctt
     7561 cttcactatc cattggtttc aatgaaaggc gtttcgtacg tacgatatct aattcagaat
     7621 cattttcgtt ttcgtcctct tcattgaata aagcagcagc atctgcagta ttcatgcccg
     7681 tttcacggga tttacgatta atttttgttt tgaatttgcg gatttggcgt tccaatttat
     7741 caacaaccaa atcgatactt gcgtataaat ctggagaagt ttcttcggca cgtaatacaa
     7801 gataaggtaa tgggatcgtt acttccactt ttgctgtttt ttctgtataa acttttaagt
     7861 tcacatgcgc cgttgcatcg ggtgcgtcac tgaaatagcg ttctagtttg cctacttttt
     7921 tctcaacgta gtcgcgaatc gcttcagtta cttcgatatt ttcgccgcgt acattatatc
     7981 taaacataaa aattacccct ttcgtctacc acaatcaaga aggagaagga ccactctttc
     8041 cctcttctta attctattat atcgaactct aggttatttg caaattaaat cgctatcaaa
     8101 tttccacatc taattcatct cgccaaagaa atggttcgga tgattttcgg ctgtgcgacc
     8161 tgcaaacaat ctgctgcgtg gaaaagagtc tgtcctgttg tatagacatc atcaataatc
     8221 aatatttttt tattttgtat cttagaaaag gtgactgcaa gttcaaaagg ttgaggcatc
     8281 tccaatcttt cctttcgcgt gttttttgct tgagccagca gatgcttctc tcttttgagt
     8341 agcatctgaa aaggaattct tgctgctttc aacatctcgc ttacttgatt gaatcctctt
     8401 tctgttcgcc ttttttcaga taaaggtatt ggacaataga tgtatgtcgg gtattctttt
     8461 tttatctgcg tccatttata tgcaaaggtg cctgctagtc ggatatctcc cataaacttg
     8521 tattgattga gccaggcctt catattttca tcgtacacaa aaaaggcacg gtgctgaaaa
     8581 tcataggtcg gatatttctt cttccattct tgacagtcac tgcagtaaac gggaatagac
     8641 tttactttct ttttcgtact ctctagattg aaatggattg gtttttgaca gccagaacag
     8701 cctcttgttt ccaataaatg aaattgtgcg cgacaaactg aacaagtctg aggtaatctg
     8761 tatttcgaaa caccaataat ctcgctgaat gttaaatttt ggaagatatc tttactgcag
     8821 caactgcacc tcacagcatc ttcttccctt cttcattcat ttctttgatt tcctgacaag
     8881 cttttcgaat agctgcagtt ttttcttcat agaaaaagta tacttgactg ttcgtataag
     8941 gccctttacg atcggctctt cctgcgattt gtaccaaaga agatttagta aaaaccggat
     9001 gatccgctcc taaaacgata acggatattc tttcaaatgt gactccacgt tcaaggatca
     9061 tcgtggttaa cagtatatca tattttgctt ctcgcatttt ctggactttt tccgcccgct
     9121 gattgtctct tgaagaaaca tctgtgatct catatctcgg aagagcctct tgaagtgtgg
     9181 ctttcaaacg atgcatcatc gtgatacttg gacaaaataa aaggatatgg ttttctttaa
     9241 ttaattgatg aatgatccgc accaaagatt ttatcttctt cttgttcttg catttttgtc
     9301 gccaattatt catgaaaacc aagacgggtt caggaagcaa acgccgatga aagcgtaaag
     9361 caagtttgtg gatgtcaaac gtctgcttga tttcctttag taacttttta tcaggtgtgg
     9421 cacttaagta gatgaatcgt ccgtctggtt taatcgctgt ttgcacagca tgagctaatc
     9481 ctgcatcccc gttataagga aacgcatcga tctcatcgat cactattaaa tcaaaggacc
     9541 gataaaaacg gtatagctga tgaatcgtac agataacaaa agaagtaaag gtataagccg
     9601 ttgctgtatt cccatggagc aacgtgattt tttcttctgg aaaaacataa cgatagcgta
     9661 aaaacaattc attacaaaca tcgactcgcg gtgtacaaat cgctactctt cctccagctt
     9721 ttaaagtttc cactaaaata ggataaagca tctctgtttt ccctgcacct gttacagccc
     9781 aaataagatg atgtcttttt tctgaatggc aaagtattct ctttgagatt tccgtttgtt
     9841 tttcagtaag ctgtcctgtc caaaaaaaga cgacttctcg aggttttgct tcaggctgat
     9901 caaacaaata taaatcttgt tgagtgtcgc atcgccctaa atatagacaa tgtgtacaaa
     9961 aataaccgta atctgcaagc tgtccatcaa tgacggacgt cctcattcca cagcgatgac
    10021 aaagaatcca gttgcccaaa cgttccactg ctgcgatctt ctcataattt ggtaaattta
    10081 cctcctcaca gtctattgct tcaatatctc tcgtcagaag ccttctaccc caaaaatgat
    10141 tcatattctt cgccctaaaa aaatatacga aaaaacaagc gatctatttt ttggatcgct
    10201 tgttcgatag ttatactgtt ctatttattc acctatgagc gtgattgctt gatctaacac
    10261 tttttcgcca ttcatcatac cataatcagc catattgatg acatctaaag gaatgccttt
    10321 tggttccaga cgtttctcaa aatcagcttt cataaagcga acttgtgggc ctaataacaa
    10381 cacatccacg tctttatttg caaggttgtt atctgcgtca gaagcggaca cagcgaaaat
    10441 atccgagtcg attccttttg cttcagcagc tttttgcatt tttgttacta gtaaacttgt
    10501 gctcatacca gccgaacata caagcatgat tgttttttta gccatacttt tctctccttt
    10561 ttaaaacaaa ctatgcaaac gttttcttcg cgtctgttac tctattatat aaaaagaaag
    10621 aaatttgtca acgttttcta aacgcggttt atttaatcaa atagccaact agatttcact
    10681 aatttattga acacgacata ttctttgtcc aaattgatga caactacttc atagcctgca
    10741 tttatccagc cataggcacc tttttcaggg tgtttataat catttgccca ccaatctgta
    10801 ctttcccttg ctgttttggg aagtccgtta ctgggaaaca gcagttcgtc aaattgggta
    10861 aaggtcaacg ttacttgaga gccgccattg ttcttcaaat aatgcgtaat tgcatcgtac
    10921 tttttcattc gttcctgcat ccccagatcc acctgccttt gtctttatta tcgcatacta
    10981 cagcaattgc gcaaccgtta cagtgttgaa acaacctaga actttattta tactgaataa
    11041 agaaaatgaa aaaaagaggt gacaagatga tcgagcgcta tcgtacaata aaagaaaatg
    11101 gacaaagtga gattgagatc aaaaaatcac gctttatctg ttctcttaga cgaatagaat
    11161 cagaggacca agcaaaaagt ttcattcaag aaataaaaaa agaacattgg aaagcgaacc
    11221 ataactgcag cgccttcatc attggagaaa aaaatgaaat ccagcgaagc agcgatgatg
    11281 gagaaccaag tggaacggcg ggcgtaccga tgttggaagt cctcaaaaaa aatcaactga
    11341 tcaatgtagc tgcagtagtc actcgatatt ttggaggaac gaaactcggc acaggcggat
    11401 taatacgagc atacactcat gcggtctctc aagcattgtc tgacattgga atcgtagaag
    11461 gtaccttgca acaggagatc tctgtgatga tcacgtatcc gcaactagga aaattacaga
    11521 attatcttga actccaacat ttttttataa aagaaatcca atatactgat caagttagcg
    11581 tcatctgtgc tgtagatgaa aaaagtgctg accactttaa agaatccatc attgagctgc
    11641 tgaacggaca agtccaattt gaagagggtt ctgtttctta tcacgagcaa ctgataactg
    11701 atcatatata aaaacaggtg attctgattc tcttttattt ctaataagga atcagggatc
    11761 acctgttttt tatttttcca aataaaactc aaaacgattt cctacgtatt gcgtacgcac
    11821 atactcaaac ggcgtaccgt cttcaaaata agaaatctgg cgcaaacgaa gaactgcatc
    11881 tcctcgtttt acctctaaat agtcagcaat tttctcagaa gccaacacag cagtaatcgt
    11941 ttgattcgct ggaccgatct ttttcccgcc tttttcttct agcgtgcgat acaacgattc
    12001 agtaatctct gctttgctgt agccatcgat cagcttttgt ggaacactgg caacttcaaa
    12061 acaaataggg acatcatctg cgaaacgaat acgttccatt cgcaagatcg tgtcatcttt
    12121 actcaagttt agtttttcca tttcacttga gcttggcgaa gtcaagaaat aagagattgt
    12181 ccgactagac ggtactcgat tttgtgattc cataatttct gtaaaacttg ttgcaccaga
    12241 cattttttct tgtacttttt ctcgcgcaac atacgtaccc gaaccgatct ttctttctaa
    12301 gattccttca tctgccaagt tttgaattgc ttgtctcaac gtcatacggc tgacattgaa
    12361 tttcactgct aattctcttt ctgaaggaag acggtctccg actttccata taccctgttc
    12421 gatttcattt tttaattggt catgaatctg tatatataca ggtaattgat tcgacataac
    12481 gtacacccgt cctttcgttt cttctattat aactggtata tacctgattc acaactgaaa
    12541 aagataaagg tttctatcca tcaacagagg atttagacac acatcgttct ccctactcct
    12601 attgtcagaa acaatttttt agatatgttc aacctagaaa ataacaaaag accctaaaat
    12661 cgatgaaaat gccgacttat ggatcttttg ttattattag cggtttttag ccatccattt
    12721 ttttgtatga ttcgttgggc gttttgattt tacctggacc gcttttccgc cttttttcgg
    12781 aatattcact tcaatattgt atttttctga tttgaccaaa tcatataact tttcagctgc
    12841 ttcgtctact ttttctttcc atttcatgtg gatccctcca attcttgtag ttaacttaac
    12901 tctatcttaa ttcagcagaa tcttcaagga acaaggtagc aaagcaaaaa aaagagctct
    12961 cgaactaac
//
LOCUS       DAFFSV010000084        12911 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFFSV010000084 DAFFSV010000000
VERSION     DAFFSV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.084"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            133..3660
                     /locus_tag="LR110_002018"
     CDS             133..3660
                     /locus_tag="LR110_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBL4822973.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="LR110_002019"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="LR110_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBL4822974.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="LR110_002020"
     CDS             7448..7789
                     /locus_tag="LR110_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4822975.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="LR110_002021"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="LR110_002021"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBL4822976.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="LR110_002022"
     CDS             9146..11566
                     /locus_tag="LR110_002022"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBL4822977.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="LR110_002023"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="LR110_002023"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBL4822978.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDTSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4385 a         2243 c         2738 g         3545 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaagtatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac t
//
LOCUS       DAFFSV010000085        12694 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFFSV010000085 DAFFSV010000000
VERSION     DAFFSV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.085"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(85..1614)
                     /gene="ahpF"
                     /locus_tag="LR110_002024"
     CDS             complement(85..1614)
                     /gene="ahpF"
                     /locus_tag="LR110_002024"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBL4822979.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1624..2187)
                     /gene="ahpC"
                     /locus_tag="LR110_002025"
     CDS             complement(1624..2187)
                     /gene="ahpC"
                     /locus_tag="LR110_002025"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBL4822980.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2331..2993)
                     /locus_tag="LR110_002026"
     CDS             complement(2331..2993)
                     /locus_tag="LR110_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4822981.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3177..4823
                     /locus_tag="LR110_002027"
     CDS             3177..4823
                     /locus_tag="LR110_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBL4822982.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4814..5896
                     /gene="uxuA"
                     /locus_tag="LR110_002028"
     CDS             4814..5896
                     /gene="uxuA"
                     /locus_tag="LR110_002028"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL4822983.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(6004..7557)
                     /locus_tag="LR110_002029"
                     /pseudo
     CDS             complement(6004..7557)
                     /locus_tag="LR110_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7705..8846
                     /locus_tag="LR110_002030"
                     /pseudo
     CDS             7705..8846
                     /locus_tag="LR110_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8972..10051)
                     /locus_tag="LR110_002031"
     CDS             complement(8972..10051)
                     /locus_tag="LR110_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL4822984.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10154..10594)
                     /locus_tag="LR110_002032"
     CDS             complement(10154..10594)
                     /locus_tag="LR110_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4822985.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10651..11061)
                     /locus_tag="LR110_002033"
     CDS             complement(10651..11061)
                     /locus_tag="LR110_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL4822986.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11241..11675)
                     /locus_tag="LR110_002034"
     CDS             complement(11241..11675)
                     /locus_tag="LR110_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL4822987.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11775..12623)
                     /locus_tag="LR110_002035"
     CDS             complement(11775..12623)
                     /locus_tag="LR110_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL4822988.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         4249 a         2360 c         2247 g         3838 t
ORIGIN      
        1 tattcaaaaa aacaaaaagg attgtgacaa aattcttgtc accatccttt tttccgctct
       61 ctgacttttt acaatcttca tagctcaagt gcgcatcaga taatcgaatg ctcctaaagc
      121 tgcagtagct cctgaaccca ttgaaacaat gatttgtttg tatggactat ctgtacaatc
      181 accagctgcg aaaacgccgg ggatattcgt tgcgccattt ttctcggtca ctatttcatt
      241 tcttggattc atttcgatcg ttccatctag ccaatccgta ttagggagta agccgactag
      301 aatgaatact cctgaaacag ctagtgtatg catctccatc gtacggcgat cgatatagga
      361 gagagattct acctgattcg taccgctgat ttctttcgtc gctgcatgtt tgataacagt
      421 gacattcgca agagagacta gtttatcctg taagatttga tctgctttta attctggtaa
      481 gaattctaga acatatacat gttttgctaa cccagccaaa tcgattgctg cttcaacacc
      541 agaattacca ccaccgataa cagcaatctc ttttccttta aataaagggc cgtcacagtg
      601 tgggcaataa gcaattcctt tatttttaaa ctctttttcg cctggtacat tgatagaacg
      661 ccagtgcgca cctgtcgcga tgatggcggt ttttgcttta agtgatatac cgttagctaa
      721 gccaatatta ataaaatgtt gctctttatt taaggaaacg gcctgttgat tcatcataat
      781 gtcgataggg taactacgca catgactttc tacttgctgc atcaattttg gaccttctgt
      841 gtaagaggta ccaatcatat tttcaatccc tagtgtttca acgacttgtc cgccgaaaga
      901 atcagcgaca agtcctgtac ggattccttt acgtgctgca tagatagctg cactactagc
      961 tgctggtcca ccgccaatca ctaatacatc ataaggttct ttttcaaaca actcttcttc
     1021 aatcaaaggg ccagtgattt tttcaacaat ttgttcgatc gtcatgcgtc cgctactaaa
     1081 ttcttctcca tttaaaaaca cagttggcac tgccatgatt tttttatcat cgatttcttt
     1141 ttggaacatc ccaccttcaa tcatcgtatg agtaatattt ggatttaata cagctaatat
     1201 attcaatgcc tgtacaacgt cggggcaatt atgacaagtc aagctaacat aactgtcaaa
     1261 atgcaatgtt tgtgttattt ttttgatttt aagactcaaa gattcactga ttttcggtgc
     1321 ccgtccacta atttgtaaca tagccagtaa aaaagaggaa aattcatgac cgagggggat
     1381 accagcaaaa acgattccac tttctttgcc aactggattg atagaaaaac taggtgtgcg
     1441 catcgaatct cttttttccc aagagatttt aggagacatt tctgctattt ccttcgtaaa
     1501 ttcagctagt ttttgactat tttcactatt atccaaacta acagaaaaaa caataggaga
     1561 ttcaatcagt gctaaatatt gttcgagatt ttgtttagtt tctttgtcta acatatccac
     1621 cacctaaatc tttccaacaa gatcaaaact tggtttcaat gtttcgccac tttctttcca
     1681 ttttgccgga caaacttcac ctgggtgggt acgtacatat tgagcagcgc gaatcttatc
     1741 aatcagtgca ctagcgtcac gaccgattcc atctgcattg atttccatag cttgaacgat
     1801 tccgtctgga tcaataataa atgttcctcg ctgagctaat ccttgctctt catccaaaac
     1861 atcaaaaata cgagaaatct gatgagacgg atcaccgatc atcacgtatt cgattttacc
     1921 gatagcatct gatgtatcat gccatgcttt gtgagtaaag tgggtatcgg tagagcaaga
     1981 aaagacttca acacctaaag atttcaatgt actgtattgt tcttgtaagt cttctaattc
     2041 agtaggacaa acaaaagtaa aatcagctgg atagaagcaa agaatactcc agttccccat
     2101 aagatctttt tcactgactt ctaaaaattc tccttgatga taagcatgtg cattaaattc
     2161 ttcgattttt ttcccaatca atgacatagt aaaacctcca ataataatta tttatttata
     2221 attattgtaa tttagttgga gttgcaaagt caatggatat tttgttattt aataaagatt
     2281 actcaaaaac gaaggttaat tataaaaata gaagaggtcg caagaaaatg ctactgctgg
     2341 aaatactcgg gataacgttg agtaacagtt tcctgttctt tcagcatcat gtgtaagtgc
     2401 aatcccgttt gaaatcgagc ttcttctggt tttttatcaa ctaaagcctt gagtaaatca
     2461 taatgttgtt ccatgatcgt ttcccatgga agcccctgaa tggtcactcg taaccagcgg
     2521 aaacgttcta aatgggtatt atggtcatct aaccagttcc aaacttcttt tcttcctgca
     2581 atttcgaaac aagcttcatg aaataaatta tctgcacgga aaaaagcgcg ttcgtcattt
     2641 attttgactg ctttttcttg ttcttctaaa atcgtttcta gcactattcg tgtttgtgtg
     2701 tccaatttgg aacaacattc aatcatgatt tgctcttcca actgttctcg gacaaagcga
     2761 gcattttctg cagaaacaag attaattttg gaaacatacg taccactttg aggtatcgta
     2821 tacacgagtt cttgctgacg tagttgaatc aatgcttcgc gaatgggagt ccggccgata
     2881 gcaagatgtt cctccagtgc cttttcagat attttccttc caggttctaa gtcagcatag
     2941 ataatttgtt tacgaatcgc ttgataagct tgtgattgca agctgctttt catctttatt
     3001 cctccaaaat catttctatg aacgtagtat aacagaagag gtctgaaaaa caatctggta
     3061 tgtcacatta actagtaaat ttgtataaat agtaaaattt tggttgacat agcgattttt
     3121 attgcataaa attcacttga atacaaaaac taatatgtta gtttaggagg ggaaaaatga
     3181 ttgatttaaa caactggagg aactttatcc ctgaattatc tcaaaaagaa gtagaaatac
     3241 ctcattttga ccgtgcatca ttaagagaaa atggacaaaa aaatcctgtc tggatccatt
     3301 tcggtggcgg aaatctttat cgaggatttc atgcagaaat cgctcaacgt ttagcggatc
     3361 taggcgaatt gtccactggt gtcattgttt gtgaaacatt cgatgaagaa ataattgaaa
     3421 gggcttacca agattttgat gatttgattc ttgaagtggt catgccaagc agtggagagt
     3481 tcgtcaaacg tatattagct gcgacagctg acagtctgta ttgtcatcca gattgttctg
     3541 aacactttga taagatgatt cgacactttg aaaatcctag tttacaatta gttacttttg
     3601 ctattactga aaaaggttat cgattgaaag atacaaataa taaatatata ggaccgattg
     3661 cagtcgatat aaaaaatggt ccaaagaagc catcacatac aatgagtatt cttgctgtgc
     3721 tactttataa acgtttttta tctggagctt ggccgattgc acttgtctct acagataatt
     3781 tttcacaaaa cggtagtcaa ttacgtactt ctttgctaac gatcgcaaaa gaatggcaaa
     3841 aggaaggtta tgtacaaaat acatttatcc aatacttatc gaatgaagaa attgttgcgt
     3901 ttccatggac gatgattgat cgtatcaccc cgaatccttc cttgtcagtt agcaaaatgt
     3961 tggcaaaaga gggcttttca aatatgtcga ttttccaaac cgcaaaagga aataatattg
     4021 ctccatttgt caatacagag gacatccatt atttaatcat ggaagatcat tttcccaatg
     4081 gacgtccgaa attcgaaaaa gctggggtat tcttgactga tcgtgcaacg gtagataaag
     4141 cagatacgat gaaggtgacg acttgcctta atcctttgca tacgtcttta gctattatgg
     4201 gttgtttact ggggtatagg agcatagctg aggagatgaa agatccagat ttatcgctgc
     4261 tagtttctga aatcgggtac aatgaaggcc tcccagttgt ggagtctcca ggtatcattg
     4321 atccaaagat ctttctggaa gaagtaatta ctgtgcgctt ttctaatcca aatattccta
     4381 atatgcctca gcgaatcgcc acggatacct ctcaaaaggt ggcgattcgt tatggtgaga
     4441 cgattaaaaa atatatggaa atagaagtta aagacccgca gacacttgta ttcataccat
     4501 tgactctcgc tgcttggctt cgatacctta ttggcattga tgatcaagga aaaccattca
     4561 tcccgagtcc agacccacta ttgacagacc ttcagcataa gctttccgat attcaactgg
     4621 gtacgcaaga tacatcacat attcatgaga aaatggctcc tattttgtca aataaagaaa
     4681 tttttggtgt agatctttac caagcagagc ttggcagaaa aatagaagaa atgttcatca
     4741 agatggtagc aggaccaaat gccgtacgaa aaacattaca ggattactta caagaacatg
     4801 gaggaaattt atcatggaaa tgacttttag atggtacgga tcacaggaag aaaagattac
     4861 cttagatgaa atcagacaga ttcctgcggt caaagggatt gtaggcacat tgatggatat
     4921 accagtagga gaggtatggc ctagggagag gatcaaagaa ctaaaagaag aaattgagtc
     4981 tcgtgggtta acactaaaag taattgaaag cgtcaatgtg catgatgcca ttaaaattgg
     5041 attaccttcc cgtgagcagt atatcgaaaa ctataagcag acaatccgaa acttggcaga
     5101 atatggaata gaagtcatct gctataattt tatgcctgta tttgactggg tgaaatctga
     5161 ccttgactac cgcttagaag acggctcatc cacgttagct tttatatctg atgatattcc
     5221 agatgatcca agagaaatcg tcgcgcgcat cgaacattcc agcaatgaat ttggattgcc
     5281 gggatgggaa cctgagcgct tagctcctat caaatccttg cttgaagcct atgcatcagt
     5341 agacgaagaa aaacttcgag aaaattttgc ctattttctc caatcaatca tcccaacatg
     5401 tgaagaggta ggggtcaaaa tggctgtaca tccagatgat ccgccatact cgatttttgg
     5461 attagctcga atcgtaaaga atcgagaaga tttagattgg ttgtgtcgca tagtagacag
     5521 cccttataac ggaatcactc tttgtacagg gagtattgca gaagatccag aaaatgatgt
     5581 atatgggatt ttagcagaat tttgccaaag agatcgaatc ccatttgcac atgttcgaaa
     5641 tattaaattt ttagctaatc gagatttcta tgaggcacca cataaatccg aatacggttc
     5701 attagacatg taccgcatac taaaaacaat gtatgacaac ggatttaacg gatacatccg
     5761 tcctgatcat ggaagaatga tctggaatga gacaggaaga ccaggatacg gcttatatga
     5821 tcgagcatta ggtgctgttt atctaaatgg attatgggaa gcaatcgaaa aaggaaacaa
     5881 aagtaaagat aattagtgaa aaaaactgta attttattat gtaaataaga aggatgttca
     5941 aattcttgat acataaatcc ggaggttgtt aataagcatt cggatttatg tatcttattt
     6001 ggtctaggaa gtaacttgat tttctttttt cgattgatcc atgagtgctt cttcttttaa
     6061 ttcaaagata tattctgaga tcctcataat cacttttttc cagtcttctg gtgtgatgat
     6121 tccttttaaa atatagattt ttttgatatg agaaggcaat tgtttcaaag ggcagtcgct
     6181 gataacgatg ttgcagttct ccataaaatc attttgtatt ttgatcgatt catttgaaaa
     6241 aatttgttct atcttaacga tcagatcttt actgatataa aaatcacgcg tatagttcac
     6301 accaatcaca attcgcggct gttttttgta gcgatcatac aaatgtacta ggtcttcaac
     6361 catccattgc acattttcct tttctaaaat aactggtaac aagtgttgat tttgttctct
     6421 gaaatccata taaaattgtt caatggcggt ttggacttct ttcaatacgc tatgttcttg
     6481 ataaagcatg aaagtaggaa ggggcgtatt tggttgattg aagttaagaa atccattata
     6541 aagtttattt cgtgcgaatg ccaatgagta aagaatcttt tctttttcag gtataaacaa
     6601 atcaaataca gtacaaaggg actccaccat ttctcttgaa taaagaacat gaggaacgtt
     6661 tgcttttttt aaaagtttgt attgctccaa gcttgtttct tccgattcga ttttcgtgat
     6721 agaaagtcga gctaaaatat tcactaagat gcgttgttcg cgacaaattg gcagattagg
     6781 tcgcaaaata tctacagaga gatcaataaa aaagtcaata tattcatact ctgtgaattc
     6841 tttttgtacg tctttcacag aagttgcagg aaaattctct ttacatattt ttaatagaaa
     6901 atataattta tctatcatac cgctatttag tcggtcaagt acttcttttt taaaaaaagt
     6961 attcatcata aaagccacta cttgcggatc ttcatcataa aattctgtag cagtacttga
     7021 aaagatgttc caataaatat ctaatgctgc atattgaatt tgtacctctt ttccagaaaa
     7081 agactttctg ccattattag aaaaggagat agacaaaccg tactatgcta aaaatgcatt
     7141 aatctttttc attttcgtat acaaataaga ggcactatag tttgtttgct gactgaaact
     7201 gataatcgaa tttgtctgac agctgaacaa agaagtcaag atctgataac tgactgattc
     7261 gttgcagtat cttccaaata gacgataata gcagtaagcc ggatcaattc cttcaaaatt
     7321 caactttaaa aaaggttttt gcttatcaat atgcagttct gttagattga gacgctcaaa
     7381 gtcttgttct aattctttga tgtatagtaa aaaatttgat tcagacatat ggaaatgatc
     7441 tgctaaatat tgtgtagtaa taaagaaacg atgttcagat aggaaaagaa aaatatctaa
     7501 ttttgtattg ctttttttct tcaagataaa cgttttaatc gcttcttgag tttgcatgaa
     7561 agaggctcct ttttacttaa attgttaaaa cttgaaaata ttactgtttt tttcataaat
     7621 ttagacaaaa aaacagtaaa catataggta gaatctctac tatcttattt cttgcataca
     7681 aaaaaatcaa ggagtgagag agttatgaga tattcaacca tcttaatatc aacatttgca
     7741 gccttaagct tatcgcttac tataaatcag acagtccaca cagaggagac agaaacagcc
     7801 aaatggacag aaaccaagtg ggccaacgtc aatcgaatta cctttcagga tgaattttca
     7861 tccttcaatt caggagtcgc aaaattaaga aaaggacaaa cagcgaccat caaagcaaaa
     7921 attgactatt cgggagactt agcacctata ctacaatcag ctgttacatt tgagaatgtt
     7981 gatcccactt tgtcgattgg ctatgacgag aacactttga ctatccaaca caagcaagcg
     8041 atatttacct tcaccgttac attaaatcag gacttgttaa aaccagcgct ttttactgtc
     8101 aaggtatcag atggactacc gaacgatcat catttaacgc cgtatagtca gcggcagaca
     8161 attgagcaag attcagccat cgactcagga ggagatcttg ttgaagaacc aacagataaa
     8221 ccagaaaatg aaaataagcc agaagtaccg cctacagaaa atccagacgg agaacagaaa
     8281 ccggaaatag aacctggaga agaacccgat acagaaactc aaccagagcc agacaataaa
     8341 tcaaaacctg aaataacccc tggagaaaaa ccagatgtaa ctccagaacc agatacggat
     8401 tctggaaacc aaacagtacc ggaaacaaat ccagacacag ataatgaaac tgaaaatcca
     8461 gaaaaaccag aggtagatcc agaagaaaaa ccagatgtga ctccagaacc agatacggac
     8521 gcaagagacc aaggaatacc agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa
     8581 aaagagagta aaaaatcaaa tctagcaatt ctaaaaataa atgaagagca gctgaataaa
     8641 aaatcacgaa tatttgatag cgcccaatca gctgaaacct taaaaagtag caaggataca
     8701 acctttgctg tcctgaaacg aaaaataaac agctaccaaa atccggagag tcacaaaaca
     8761 aagttatact gtggagcgga atcattctat taagtatagc aacaatgctt tctgcaaaaa
     8821 gatttaaaca aaacagatct ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa
     8881 taaaaagaaa tatccagaaa atgaccccaa aataccagat tgaaatctga tatttggggt
     8941 cgttcatact ttatctttct tcatatttat tttaagggat atctgtttct tttggaagat
     9001 ggttcgggat ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga
     9061 caatcaagcc ccaaacgcca aagaaccgtt cgctaacaag taagaccaca aatgtataaa
     9121 aaatcggcag ttctgttttg ctggacatca acttcggatt cagcacataa gactcaatta
     9181 gatggacaat caaaatcgta atcaggatat aaacaacatc tcggatgcct ccttgggaat
     9241 aagctatgaa agatagagga atacaagaaa tgataacacc tgctactggg atcagactta
     9301 aaatgaaaat catgatcgct aaactcagca gctgatgaaa gccaaataca gccaaggcga
     9361 ttgtagtgag caacgtattt aaaagagcga caacaaattg agcttctaat accaaaccaa
     9421 atgtattgac aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac
     9481 tttttaaaaa caatcttgag aatacaattg tttttttctt ttcaatcata aaaaagaaac
     9541 ttaagatgaa tgacatagca aacgccactg ccatttttcc tatatcctgt aaatagccaa
     9601 gcgcaatcga agcgccattt tgaagctgtt caaaaaaatc gtttcgctca atataagtat
     9661 ggatatattg taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa ttaataacgg
     9721 actggatcat gtcaaatgtt tgatgaataa gcatcgggat atactttgtg aatgccagat
     9781 agataaggta aacgatcagt gtataaatca tgatagctaa aacacctgta gggattcgga
     9841 caaatcgctg aatgatttga acaagatgaa tcatcaaata agtaaagata aatgtgagca
     9901 aaatggttgt aatcatactt ctaactaaga aaagaacaaa aacgacaaat aataaaacag
     9961 tgagacggcg tattttttca ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct
    10021 ccaaataaca aaaaataaac acgttattca ttatatgatg gaatatgaaa ataagaaaga
    10081 gcaaaaagaa aaaccgctaa atgacgttat cttatgagtt ggttttcctc ataaaatcgt
    10141 tcatctagca gtttcagccc tttttctggt cttgatgaaa attatctagc atttttcggg
    10201 tcagatgttg cagtgtatcc aattcttcct gttcaagtgg aagataagac ttgatctttt
    10261 gtggaatacc ttctgcttgt ttttgcaacg cagcaccttg agcagtcagc gtaacattta
    10321 cgattctttc atcacccgtt tcacgctgac gattaatata atttttttct gctaactttt
    10381 ttagtaaggg agtcagcgta ccagaatcca gacacaaatc atttcctagc tctttcactg
    10441 aagtcttttt cttttcccat aaaactaaca tcacaagata ctgggtatag gtaagattca
    10501 gcggcttgag gaggggagtg tagtgtctaa tgatttcttt agaggcggca tataaaggaa
    10561 aacatacttg attaactaaa tatagatctt ccatttttcc acctccgctg ttttactata
    10621 gtgtatcata gcggatttac acaaaaaagt ctattcacta actgctttca gagtaacttc
    10681 tatatttcca gctgtagctt ttgaatacgg acacacttta tgcgctgcct ctaataattc
    10741 ttgcgtttct tccagagaaa gaccttcgat agatccttca atctctacac ctaagacaaa
    10801 gctcgtttca ctttctgaat aaagggaaac ttgtgcactg acagtagaag ccccagagat
    10861 atttttttgc tgtttgatca attctaatgc actgttaaaa catgcactga aaccagctgc
    10921 aaacaattgt tcagggttgg ttgcattttc gacgcgttta ccaggagctg caatattcaa
    10981 ttcgaatgta cgatcgggat tatgcacttc tccagagcgt ccgcctgtgt tgatcatttt
    11041 cgtagaatac atttttttca taaaattacc acctttaatt atattttata caattgaatt
    11101 ttacacaata taattgtgga aagcaaagaa atggattggc ataagataaa taagagaaag
    11161 cacatgaaac agacgcgctt tctcgaatag caaacatata caaaaaatat cagagacaaa
    11221 aaataatctc atcataaaca ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa
    11281 attacttcac gatggacctg atgaagcatc cataaataac caaaaggatc tctgaacatt
    11341 gagttagtca caccaaagtc catcatttct gtcatttctt gaatctctac acagccattt
    11401 cgtaatgctt tattatgtgt gtcctcgata tcagagacga tgatattgaa ccagatcgta
    11461 tttggatgtt cagcagtagg agcagtcaaa ccaagagacg tgttttcatc cagcatatgg
    11521 aatctcgtac catacaaggt aaaaatgact tcattattgc ctctagggta atctgttgcc
    11581 tcaatcagtt gtatttcatc aaagattgtt tgatacagtt ctagtgcttt caaactatca
    11641 tttaatacaa aatcgatttc cgttccttta tgcataaaga tctcctctcc ttttcagtcg
    11701 aacagttact agattctttt attataccag ctaaagaaaa cgcattcaaa aaaatagcgg
    11761 atttacttac caactcattc gattggatga taagtaaatc cgcgtcccat tacttctgct
    11821 acgctgctga caatgacaaa ggcacttgga tcgatttctt ctatcaatgg tttcattcgg
    11881 gaaaattcat gatcagtcac aacgatcaat aaaacctctt ttgactttcg attatacccg
    11941 ccttttgcct ctagcagggt aaggccacgt ccaatctcag cacttaagcg aaggcggatg
    12001 tcttccaaat aatgttcact catgaccata acagatttct ttcgattgat acctgtttcg
    12061 atataagtca tgacaattga agtgattgca atagataaaa tagcataaag aaactcttct
    12121 attccaaaaa taaacagatt aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc
    12181 gaagtattca agtggaaata ttttttaaaa atgaggggag ggatcgtcgt accgccgcta
    12241 gaagagttgt ttttatataa aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg
    12301 acagacaaca accgatctga agtaatcatt gtttctggaa ctattgcaat gattaaagga
    12361 aaaaccaagc ttccgaaaag tactttaaaa aagggttttt tacctaaaaa tagaagagct
    12421 agtaccaaca taacgatatt caacactaaa ataactatag ctcggtcaaa gcctaaagca
    12481 gcttccagta atattcctag accgctgaca cctccagcag caatatgatg aggccctaaa
    12541 aacatattga tagcgactcc taatatgaag agggcaaaaa taatcttaat gtatgtccat
    12601 ataaattgtc tcgtttcttt catttattca cttccttaat tcattaataa tactcttatc
    12661 ttattataac ttacttttca ccttaagtta actt
//
LOCUS       DAFFSV010000086        12667 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFFSV010000086 DAFFSV010000000
VERSION     DAFFSV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.086"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            186..755
                     /locus_tag="LR110_002036"
     CDS             186..755
                     /locus_tag="LR110_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBL4822989.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            742..1602
                     /locus_tag="LR110_002037"
     CDS             742..1602
                     /locus_tag="LR110_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL4822990.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            1792..2496
                     /locus_tag="LR110_002038"
     CDS             1792..2496
                     /locus_tag="LR110_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4822991.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2501..4336
                     /gene="walK"
                     /locus_tag="LR110_002039"
     CDS             2501..4336
                     /gene="walK"
                     /locus_tag="LR110_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBL4822992.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4333..5649
                     /locus_tag="LR110_002040"
     CDS             4333..5649
                     /locus_tag="LR110_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4822993.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5650..6519
                     /locus_tag="LR110_002041"
     CDS             5650..6519
                     /locus_tag="LR110_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBL4822994.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            6574..7383
                     /locus_tag="LR110_002042"
     CDS             6574..7383
                     /locus_tag="LR110_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL4822995.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7486..8115)
                     /locus_tag="LR110_002043"
     CDS             complement(7486..8115)
                     /locus_tag="LR110_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBL4822996.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8285..9577
                     /locus_tag="LR110_002044"
     CDS             8285..9577
                     /locus_tag="LR110_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4822997.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9653..10315)
                     /locus_tag="LR110_002045"
     CDS             complement(9653..10315)
                     /locus_tag="LR110_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL4822998.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10540..10824
                     /gene="groES"
                     /locus_tag="LR110_002046"
     CDS             10540..10824
                     /gene="groES"
                     /locus_tag="LR110_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBL4822999.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            10875..12500
                     /gene="groL"
                     /locus_tag="LR110_002047"
     CDS             10875..12500
                     /gene="groL"
                     /locus_tag="LR110_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBL4823000.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4210 a         2179 c         2755 g         3523 t
ORIGIN      
        1 ctcctttccc gccgtctccc cggcgggtct cctgttctta acgataaaag acaagaaggc
       61 ttagccttct tgttttttgt tttcatggaa tcccgctaat caaatcacct agttaaagca
      121 actagattga atgtttatca taagtctgat atcatgttta tgaaatgaaa aaaggagcgg
      181 ttatgatgga aaaaatgcgt cagcaattaa tggagtgggg gaaggaacta gaaacattcc
      241 gtctgcctca ctgggaagaa ctccctgact tagaacttta tatggaccaa gtcagaacgt
      301 tagttgatcg ttatctttca cctgtgatac aaggagaaaa gcatccattg cttacctctt
      361 ctatggtaaa caattacgtt aaactaggtt tgataccagc accagttaaa aaaagatata
      421 acaaagaaca cgtagcattt ttacttgcaa tcactacgtt gaaacaagta ttgactattc
      481 ctgaaatcaa agaagggata ttgttccaag gaaaaacagt aggaattcgt gaagcctata
      541 atctattttg cgatgaacaa gaagcagctg tgtggatggt cagccaatta gcacaaggta
      601 aatctcatcc gcaaaaattc gaccaggcaa cccctgtaga atatattgct gtaagagcgg
      661 caactttatc gtttgcaatg aaacttcttg cggaaaaaac gattgttcta gaaaccgaat
      721 atttaaaaga ggagaaaaca aatgaaaaac aataaaatcg caattttagt agattcaggt
      781 acggatgttc cagaagaatt gataaaaaaa tacgagatgt atatgatccc attgaaaatt
      841 atttataaag atcaggtata tacagataaa attgacatta cccctgaaga gatctatcaa
      901 cgtttgccag aggaaatccc tggtacatct ttgccggatg gtgagtcgat cacagaaatc
      961 tttgagcaaa tcaaaaaaga cggttatgaa aaggtacttg cggtaacaat ttccagtgga
     1021 ttgagcggga catataatgt ggttcgttta attgcacaac agcaagaaga attggaagca
     1081 tatgtactag atacgaaaaa tattggtatt ggtgcaggtt tttctgcgat ccaagcagca
     1141 aaatggctgg aagaaggagt agaatgggat caactgatca gtaatctgaa tgagttagtc
     1201 gaaagaacaa aagttttctt taatgttgcg acattggaat atcttcaaaa aggtggacgg
     1261 atcggtttag ttgcttctat tgtaggaacc gctttaaagc ttaacccaat catctcatgt
     1321 aatgaagaag ggatttatta cacagtagga aaagctaggg gacgtaaaaa aagtttggat
     1381 aaaacggttg cttacgtcaa agaaaaaatc ggtaatgcaa aggtcttcaa cttagctgtt
     1441 gcccatgggg atgcgaaaga agaagcaatt gaaatggaag cgagacttaa aagagagttc
     1501 ccacaagcaa accaaatcta ctttggccag atttctccgg cattagtagt ccacactggt
     1561 ccgggattat tgggtgtcgg tgtacaagtt ttagaaggat aaatcaaaaa aaggaaacac
     1621 tgaggtgaaa atcagtgttt tcctttttta ttgaagttag aacaaaaaat aataaagtat
     1681 tttgcacttt cattttaatt ttctcttttg ttttataggc aaagatgaaa caaaagcaga
     1741 aatacggtat aatgagaagg tatgacaata atatataaga ggtggctctt tatgaaaaaa
     1801 gttttagttg tcgatgatga gaagccgatt tcggatatcg tcaaattcaa tttagccaaa
     1861 gaaggctatg acgtttatac tgcatatgat ggggaagaag ccttggaaaa agttgctgag
     1921 gtagaacctg atttgatttt acttgatttg atgctaccta aaatggacgg cttagaagtt
     1981 gctcgtgagg tacgcaaaac ttatgatatg ccgatcatta tggtaacagc caaagattct
     2041 gaaattgata aagtcctagg cttagagtta ggcgccgatg attacgtgac caagccattt
     2101 tccaaccgag aattagttgc acgtgtcaaa gcaaatcttc gtcgcggtgc cacggctgcg
     2161 aaggaaccag aagaggcagc acctgcggaa ttgacgattg gtgatttgac gatccatcca
     2221 gaagcttaca tggtgacgaa acgaggagaa acaatcgagt taacacatcg tgaattcgaa
     2281 ctactctttt acttggcgaa gcaccttgga caagtgatga caagagaaca tttattacaa
     2341 acggtttggg gttatgatta ttttggagat gtacgaacag tggatgtaac tgtacgtcgt
     2401 ttgcgtgaaa agatcgaaga taatccaagc catccaaatt atttagtgac acgtcgtggt
     2461 gtcggttact atttgcgtaa cccagaacag gagtaattgg atgaaaagaa aagtccgctt
     2521 ttttcgatca gtcaatttta agatcgctat tacctttatt ctgattttat tgatctcgat
     2581 cgaaattatt ggtgcctact ttatacgtgg tttagaacgt tccacgatca atacatttat
     2641 taaagatatg aatcaaacgg tggaatcatt agcaacaaca atcagcccgg aattgaatcg
     2701 aaaagataat gcagatgatg aagaggtaaa tgcgaatatc aaaagattta ttgaaaacag
     2761 tgcgactagc gatatcatcg aaatacgggt cgttgacgaa aaagggatca tccgaggaac
     2821 aaccgatgtc aatgaacaaa gcgctgtggg aaagaaaaat gattatgttg atatcaatga
     2881 tttcacgact aaacgttatg ttgctttaga taacgacgat aaacgggtga atatcaatgt
     2941 tcaaccgatc ctgtccccga ccggcgatgc cgttatcggg gctctttacg tcaaaagtaa
     3001 tatcgaacaa aaatattcgg aaatcaataa cacagcagtc attttcttta ctgcctcact
     3061 aattgcggca tttatttcaa tgatcgtatc tgttttagtt gctcgttcga ttacacagcc
     3121 gatcggtgaa atgagagaac aagccattcg aatcgctaga ggagactaca gtcggaaagt
     3181 caaagtgcat ggacaagatg aacttggaca gctggctgat accttcaatc agttagctga
     3241 acgtattgaa gaagctcaag atacaatgga agctgaacgc aatcgtttgg atagcgtgct
     3301 atcccatatg acagacggcg tcattgcgac agatcgcagg ggtaaagtca ttacgatcaa
     3361 tgatatggct gtttccttgc tagatatcaa aaacgaggaa gcaatcggac aatcgatttt
     3421 gacactttta gatatcgaag aagaatatac gttgcggaaa ttattagaag atccaaacga
     3481 aatggtactt gaccgttcaa ccagtacatt ggaaagtgat cagatgatct tgcgagtaga
     3541 cttttctatg atccgtcgcg aatcaggttt tatcagcgga ttagtagctg tcttgcatga
     3601 tgtgacagaa caagaaaaaa cagaacggga acgtcgtgaa ttcgtctcta atgtttccca
     3661 tgaacttcgt acaccgttga caagtatgcg tagttatatc gaagcattga gcgaaggtgc
     3721 gtggaaagac gaggaagtag caccaaattt tttgaaggta acgctagatg aaacagaccg
     3781 aatgattcga atgatcaatg acctcttaaa tctatctcgc atggatacag gtaataccca
     3841 gcttcaatta gaatatgtca atttcaacga gttagtgaat tttgttcttg accggtttga
     3901 tatgatggtg ggaaatcagg aaaaaaatta taagatccgt cgtgaattta cgcaaagaga
     3961 tctttgggtc gaagtagata cagataaaat catccaagta gtcgataata tcatgaataa
     4021 cgcgatcaag tattcaccag atgggggaac gattacttgt cgcttattag aaacgcataa
     4081 taacgtgatt ttaagtatca ccgatcaagg attggggatt cctaaaaaag atctgaaccg
     4141 agtgttcgag cggttctatc gagtagataa agcacgtgca agagcacaag gtgggacagg
     4201 acttggatta gctatttcta gagaagtgat taaggcgcat cgaggcgcta tttgggcaga
     4261 gagcaaggaa ggaaaagggt cgacttttta tatctcgctg ccatatgaac cttatgagga
     4321 ggaatggtgg gaatgagaat ctctgaaaaa gtcgttcgta taggactcat tttgatggtt
     4381 gcgttaagca tatacttctc ttatgcaatc tggctgagcc cagcaggaaa aagttcaatc
     4441 aacgtagatg agacaaacac tcaaatgatc gagtctcaaa gcaatcgaaa aacttccgaa
     4501 gtctttcttc cgcttcatgt tacatggcac cattcgggaa tgatccaaga aaccaacagt
     4561 gaaaatctcg tgtaccgatt gcaattgatc atcgataaag caagatttgg aaagctttct
     4621 gaagtagttt caggagacaa atcacagttt gaacaggtga aaaatctgaa ctctggtttt
     4681 gagttggcct ataatgcgcc ttttttgcta agagaataca aggaagcatt tgatttagat
     4741 ttagatgtgt cgaacttggc aaaagaaagc gaaaatatgt attttactag aatacaattc
     4801 gatcaaggaa ataaaaaaat ccgatttttg aattattcga accatctcgt atatgaagca
     4861 aatatttcgt tgaattggac agatatcaat aaagaattaa cagcaaaaga cacaaaatgg
     4921 acagaaatga caacagaacc atcgacgatc gacacgcaat atgctacgaa acattcgatc
     4981 aaactgaaga agtatagtta tatcctgtca cagcaaccct atacactttt tagaaatgct
     5041 tttttccaga aaccagatag cgtgaaaaat aatgatgaaa gcaatgaatt gattttttac
     5101 ggtgggaatg aaacaatgtc tatccattcg gaaacgcaag tagttgattt tcgtggagag
     5161 ctgccagaga aaaaagaaaa tgctagtgta ttttctgaaa gtttccccta tattagcaaa
     5221 ttaggcagtt ctttagggaa cctcagatat tttgaccgca gtgacaatac gatcgattat
     5281 cggatattcg tggaaggctt cccggttttt gggacagaca gcagaggaca gattggtgtg
     5341 gaagttggcg gtgaaacgaa tggttctgtg caagtgaata tccaaaccag tttgaatacg
     5401 attcaagtgc cgataccttc agacgaagaa gtagaacttg caagtacgaa tgaaatcatc
     5461 cagcagttaa aagctaaggg agcagatagc aacaagattg gttcagtgat cgttggctat
     5521 acatggcaaa atgtcaaaga aacgaatcgt gtcgttgatc tcttacctga atggtatgtg
     5581 aaatataaag ataactggta ttcagctaac gaccttctgg aaaaattacc tggattggag
     5641 gcgaattaat tggatttcaa aaaaatcgaa tggatatttt tcgtcgcttt tttagggttg
     5701 aatgtgtttt tattcagcag ttatcatgac gcggtatacc aagaaaacaa tgtcattcgc
     5761 tctaatcaga ctgaatcaat ccaacaacgt ctggctagcg acgatatcac ttacacgggg
     5821 aagatttcgt ctgagaacaa gcaaggctat tatcttagcg cggagcagac caatcttagc
     5881 acttcattag cgaattatcg caaggctggt aaaagcggct tgctggcaag tggtgtgttt
     5941 ttagacggtg atgttttgac gaaatcttta tccagttcag atgctgaaaa gaacgtgatc
     6001 gatccagata aaattagtag ttccttggaa gattttttat ctaacaaaga caatatttta
     6061 ttcggtaatg attatgtgta cacgaaaaac ttttctcatt tagaagaaga accactagaa
     6121 atccaagcaa gccaagagta tgaaggcatc ccattcaatg atgatacagc aaaaatcgtt
     6181 ctttctttag aaaaaaacga taatgattat agcatcacga aatacatgca gacgcattta
     6241 tctgatatcg agcagctgcg cgaaaagacg gaactgtata cggaagagga agcaatcaat
     6301 acattgtaca tcaataacaa aataccaaga ggatcaaaaa ttttatggcg tcagctagct
     6361 tattcctgta ttctgaaagt ccgggaaaaa aatgtgtatg ttcctgtttg gcatgtagcg
     6421 atcgaaacaa gcgataaagt ggtccaaatt gagagcatca acgctttcag caatacgatc
     6481 gtgacaaaca atactgtacc aaaagtggaa gatcaataaa aaagcgagta taatgaaaaa
     6541 agtttacaaa gtaagaaggg aagtaggagt caaatggctg aagaaaaacc agcttttaaa
     6601 atcagtatcc tagcaagtgg aagttcgggt aattcacttt atatcgaaag tgaaaaaaaa
     6661 cggcttttgg tcgatgcagg acttagcggt aaaaaaatca cttccttgat ggctcaaatt
     6721 gatcggacac cggaccaatt agatggaata cttgtgacgc atgaacatcg agatcatata
     6781 catggcgtgg gtgtcttggc tcgtaagtat catttggatg tttttgccaa tcagcagact
     6841 tgggaagcga tggaaccact gattggaaat gtaccagtgg aacaaaaaca tctatttgag
     6901 atggggaaag tcagaacatt cggcgatatc gatattgaga gtttcggcgt ctcccatgac
     6961 gcagcggctc cgcaatttta tcgtttccat aaagaaaatc ggtcattcgt catgttgacg
     7021 gacaccggat attgcagtga ttatatgcga ggtgtcattg aaaatgccga tggttatttg
     7081 atggaaagta atcatgatct tgagatgtta cgcatgggac cttatccgtg gaatttaaaa
     7141 cagcgtattt taggagatag aggacatctt tccaacgaag acggtgcact tgtcatgact
     7201 gatgtgatcg gtaatcggac caaacgaatc tatcttggcc atcttagcaa ggaaaacaat
     7261 atgaaagaac ttgctcattt aacaatggaa aatgttttga aagaaaaaga ttttggcgtt
     7321 ggacatgatt ttgaaatcta tgatacagat ccagatgaag caactgagct attttcgatt
     7381 taaacagttc aatcaataaa aatataaaaa gcagaagata aaatcaatca gggttggttt
     7441 tatcttctat ttttattttt ttagcatatt aaaaaaataa ccgtattatc gactgactac
     7501 actgatgtgc tgcttttcat gtttctcatc cagtatctca tctactaatg caatcgcgta
     7561 atctgccatg ctgatcgtac tgtcgctagc agcgtttctt tgtaaataat catcacttaa
     7621 caaataagta cctgtacgtt tgccatgtgg atcaaataaa gaagctggac tgatataagt
     7681 ccaatgtaga tttttagatt gtttcaattt taacaatcct tcgtacatat tgaaagctgt
     7741 tggatagaag ggtgcatttt taggtacttg gtcaatcata cgagtcgtct tttctgtatc
     7801 taagaataac gaagaggcac ccccaacgac aattaagcgt gtatcggttc cttcaagcat
     7861 ctcaatcaaa tgggacaaag aagtttgatg catttcttct tttcctttgg gagcgttgaa
     7921 agcatcaacg acaacatcaa aagatgcaag atcagtagtt gtcaactcaa ataaatcctt
     7981 ttgtataaac ggaacatctg atggcaattt ttctggatga cgcacgattg ccgtcacgtc
     8041 taaatctctg gacaatgctt cgtctaaaat aaatgaacct gcatggccag tagctccaat
     8101 gagtgcaatt ttcataaaaa acctcctttt ttattcctgt ccattgtaag cagttgctga
     8161 atattcgtca aagaatccga tttttgaaaa gctcagtgcg cattcctttc atggttcgtg
     8221 atcattgggt atagttaaat taagaaaact gattgataaa aatagaagtt gaaggagata
     8281 agaaatgaaa gagtggttag atcgtgcctt tttaccaatg ataacatctg ttgtgaatct
     8341 tagaccctta actgctttgc gtaatggaat gacttacacg atcccaataa ttattgttgg
     8401 ctcgctgttt ttcttatttg ctcattttcc tgtggattac atcgctcagt ggatagacaa
     8461 aacaggattt tctatgtatt ttacacaagt ataccaggct acatttgatg tgatagcctt
     8521 ttggatcgtt tttgccattg catacgctta tgcaaaagaa gaagacatcg atcgcttacc
     8581 agtcggcata acagccttgt ctgcttatct actgcttatt cagccactaa atactcagat
     8641 agatactgtc tggacaggtg ctgaagggat actaggagca gttacaactg ggctcatcat
     8701 cggatggggc tatgcacgtt tagtaagact tgcgaaaggg tggaagcaaa gagagaagat
     8761 acctgaaaat gtttggcagt cttttacttc attgattcca atcattgtta tagtcacttt
     8821 tacgcttatc ggttcagcgg tttttcaaca tttgttcaga atgactgttg ttcaaggagt
     8881 atggacatat gtccagcttc ccattcaatc gttcatagac acgttgtttg gtgtgctttt
     8941 attaggtttt tttgttccgt ttttatggtt atttggtctt catggatctg cgatggtcaa
     9001 tgggttggtt agtcccgtct tgcaagcgaa ttcattagca aatgcagaaa tcttagcctc
     9061 aggacaagag ttgacggtgg caaatggcgg acatatcgtt actcagcaat ttctcgatca
     9121 gttcatgacg gttacgggtg ctggattgac gttaggggct gtatttttta tgacggtttt
     9181 tgcgaaatcg aagaaataca gagaattagg aaatcttgct cttttaccag cctttttcaa
     9241 catcaatgaa tcaattattt tttccacacc gatcgtgatg aacccgatga tggctgttcc
     9301 ttttattttc gcaccggtcg tttcaggatt gattacctat tccgctttat attttggatt
     9361 cgttccttta tttacagcag tccaagtccc ttggacaaca ccgccgatcc tctcgggttt
     9421 cttagtaggc ggagtgcctg cagcgatttt acaaggtatc gtgttgtcaa ttagtttttt
     9481 catttatttc ccatttttga aaaaaataga ttctgcaaac tgcaaaaagg aaagacagac
     9541 aaaaaatgat ggagcagcag aaaagatgaa cgactaaaaa acaaaaatac tcctgtcgct
     9601 ttagattggg aaaatcacca atctaggatg acaggagtat ttatagtcat gtttatacta
     9661 gccctttttg gacagctaat tgaacaagaa caactaatgt gttcattcct acgtgtgtga
     9721 tcatcgatgt ccagatccga ccagttgctt tgtactctag gctgaagaag aaacctagga
     9781 aaaagtagat tagcagatga ccatcattat gagcaaacgc aaacagcaaa gagctgatca
     9841 ctgctccgac ccaaaaattt gaatatcgac tgatgatccc cagtatactg cggcggaata
     9901 cgaactcttc catgatgggt ccagcaacag ttgtcgccaa aataaaagcg ggtgcttcca
     9961 tgatcatctg gatgatattt tgtgtattct cggaaggggt tgctttgcca aacaatacag
    10021 attctaacat gacagcgatg ctttgcagaa tcaatgcgac aaagattcca accaatccat
    10081 aaataacaat ctttggagca gaagcttttt ttaccgctga ttcgatcgct aagggttcac
    10141 gttgcttaaa ataaaaaata gctaggacaa cggcgcctag tatatacatg actgcagttg
    10201 ctgtgatcaa ttcagacgta gattttacaa gtcctatcga agctaggatc aagggtgaga
    10261 taaaaattag accatagcaa aaaatgctga ggaagctata tttttttaaa gacatgtaat
    10321 tcctcctttt aagggataac aagcatgcaa ggcaagctta tatcgattga tttcaattat
    10381 atcataattt caataagcag ctgtcagaat aaaatttctt ttctttgatt taaaagcttg
    10441 caaaaaagag ggagaatgag tattattaaa aatgtaggtt agcactcgaa ggttaagagt
    10501 gctaacagat taaaaaaaga gttatcggag ggatttatcg tgttaaaacc attaggtgat
    10561 cgcgtcatta tcgaagtcgc aaaagaagaa gaaaaaactg tcggaggaat cgttttagct
    10621 tctgctgcta aagagaaacc ccaaactggg acagttgttg cagtcggtga aggccgtttg
    10681 ttagaaaacg gtgaaaaagt tcctgccgct gtaaaagcag gcgaccaagt tatgttcgaa
    10741 aaatacgccg gtacagaagt aaaatacgaa ggaaaagaat atctgatcgt cgcaggaaaa
    10801 gatatcatgg cgattgttga gtaaatttgg aagataaaaa aataatcaaa aataatcaat
    10861 gaggtgaatc aatcatggca aaagaattga aatttgcaga agacgcacgc gcagctatgt
    10921 tacgcggggt agataaatta gcagatacag tgaaagtaac attaggacct aaaggccgaa
    10981 atgtcgtact agaaaaatct tacggctctc cattgatcac aaatgatggt gtgacgatcg
    11041 caaaagaaat cgaattagaa gatcattttg aaaacatggg tgcaaaactt gtatcagaag
    11101 tagcatcaaa aacaaatgat attgcaggag acggtacaac gacagcaact gttctgacac
    11161 aagcaatcgt tcgcgaaggt ttaaaaaacg taactgcagg tgctaatcct ctaggcattc
    11221 gtcgcgggat cgaattggca acaaaagcag cagtcgaaga attgcataat atctcaactg
    11281 ttgttgattc aaaagaagcc attgcacaag tagcagctgt ttcttcgggt tctgataaag
    11341 ttggacattt gattgctgat gcaatggaaa aagttggaaa cgacggtgtc atcacaatcg
    11401 aagaatcaaa agggatcgaa acagaactag atgttgtaga aggtatgcag tttgatcgtg
    11461 gttacttatc acaatatatg gtaacagata acgacaaaat ggaagctgtt ctagaaaacc
    11521 catatatctt gatcacagac aaaaaaatct ctaacatcca agatattctg ccattgttag
    11581 aacaaatctt gcaacaatca cgtccattgt tgattattgc tgacgatgta gatggtgaag
    11641 cattgccaac tcttgtattg aacaaaatcc gtggaacatt caacgtagta gctgtaaaag
    11701 ctcctggttt cggcgatcgt cgtaaagcaa tgcttgaaga tatcgctatt ttgacaggtg
    11761 gtacagtaat tactgacgat ctaggtttag aattgaaaga tgcaacaatc gaaaacttag
    11821 gaaatgcttc taaagttgtt gtagataaag acaatacaac gatcgtagaa ggttctggag
    11881 aaaaagaagc aatcgaagct cgtgtgcaat tgatcaaaaa ccaaattgct gaaacaactt
    11941 ctgattttga ccgcgaaaaa ttacaagaac gcttagctaa attagcaggt ggtgtagctg
    12001 ttgttaaagt tggtgcagct actgaaacag aattgaaaga attaaaatta cgcattgaag
    12061 atgcattgaa tgctacacgt gccgcagtag aagaaggaat ggtctcaggt ggtggtacag
    12121 ctctagtgaa cgttatcagc aaagtttctg ctgtagaagc agaaggagac gtggcaacag
    12181 gtatcaagat cgttgttcgt gctttagaag aaccaattcg tcaaatcgct gaaaacgctg
    12241 gttatgaagg atcagtgatt gttgataaat tgaagaacgt ggaactaggc actggtttca
    12301 atgctgctac tggagaatgg gtaaacatgg ttgaagcagg tatcgtagac ccaactaaag
    12361 taacacgttc tgccttgcaa aatgccgctt ccgtttctgc cttgttatta acgacagaag
    12421 cagtcgttgc agataaacca gaaccagcag cgccagcagc tcctgcaatg gatccatcaa
    12481 tgatgggcgg tatgatgtaa gaaaaagcaa cttgcttatc tacaactgat cattagtgaa
    12541 agacagataa aaagaggctg gaacagaagc gtttaactcc aagaaataag aagaaattca
    12601 cggaaattgc ttttcaaatt tttgtgaatt tcagcttatt tccgaaggag ttgcttctgc
    12661 ttccgct
//
LOCUS       DAFFSV010000087        12573 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFFSV010000087 DAFFSV010000000
VERSION     DAFFSV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.087"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="LR110_002048"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="LR110_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBL4823001.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="LR110_002049"
     CDS             808..1242
                     /locus_tag="LR110_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBL4823002.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="LR110_002050"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="LR110_002050"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBL4823003.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="LR110_002051"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="LR110_002051"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBL4823004.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="LR110_002052"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="LR110_002052"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBL4823005.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="LR110_002053"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="LR110_002053"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBL4823006.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="LR110_002054"
     CDS             9513..10439
                     /locus_tag="LR110_002054"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBL4823007.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="LR110_002055"
     CDS             10621..11514
                     /locus_tag="LR110_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBL4823008.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="LR110_002056"
     CDS             11648..11773
                     /locus_tag="LR110_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4823009.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="LR110_002057"
     CDS             complement(11907..12539)
                     /locus_tag="LR110_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823010.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         4517 a         2129 c         2636 g         3291 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aag
//
LOCUS       DAFFSV010000088        12516 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFFSV010000088 DAFFSV010000000
VERSION     DAFFSV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.088"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            14..751
                     /locus_tag="LR110_002058"
     CDS             14..751
                     /locus_tag="LR110_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL4823011.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            765..1580
                     /locus_tag="LR110_002059"
     CDS             765..1580
                     /locus_tag="LR110_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL4823012.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1593..2240
                     /locus_tag="LR110_002060"
     CDS             1593..2240
                     /locus_tag="LR110_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL4823013.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2256..2912
                     /locus_tag="LR110_002061"
     CDS             2256..2912
                     /locus_tag="LR110_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL4823014.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3074..3895
                     /gene="racE"
                     /locus_tag="LR110_002062"
     CDS             3074..3895
                     /gene="racE"
                     /locus_tag="LR110_002062"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBL4823015.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3898..5253
                     /gene="rph"
                     /locus_tag="LR110_002063"
     CDS             3898..5253
                     /gene="rph"
                     /locus_tag="LR110_002063"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBL4823016.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5254..5772
                     /locus_tag="LR110_002064"
     CDS             5254..5772
                     /locus_tag="LR110_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL4823017.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5879..6379
                     /locus_tag="LR110_002065"
     CDS             5879..6379
                     /locus_tag="LR110_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL4823018.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6659..7579)
                     /locus_tag="LR110_002066"
     CDS             complement(6659..7579)
                     /locus_tag="LR110_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBL4823019.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7744..8757
                     /locus_tag="LR110_002067"
     CDS             7744..8757
                     /locus_tag="LR110_002067"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBL4823020.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8867..9622)
                     /gene="fabI"
                     /locus_tag="LR110_002068"
     CDS             complement(8867..9622)
                     /gene="fabI"
                     /locus_tag="LR110_002068"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBL4823021.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9842..10282
                     /gene="fabZ"
                     /locus_tag="LR110_002069"
     CDS             9842..10282
                     /gene="fabZ"
                     /locus_tag="LR110_002069"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL4823022.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10397..11158)
                     /locus_tag="LR110_002070"
     CDS             complement(10397..11158)
                     /locus_tag="LR110_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL4823023.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11347..12441
                     /locus_tag="LR110_002071"
     CDS             11347..12441
                     /locus_tag="LR110_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL4823024.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         4029 a         2173 c         2641 g         3673 t
ORIGIN      
        1 ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg gataaatatt atggcaagtt
       61 ccacgcatta aaaaatatca atctcgaatt cgagaaagga gaagttgtcg ttgtcattgg
      121 tccttctggt tcaggaaaga gcacgatgct tcgctgtatc aatggattag agacgatctc
      181 ttcaggtaag ctgctgatta atgatactga cttgcatgat aaaaagacaa aactgacaga
      241 agtccgaaaa aacatcggca tggttttcca acatttcaat ttgtacccaa ataaaactgt
      301 tctggaaaac atcaaattag caccaatcaa agtgttgaag caagaccagg caactgcagt
      361 caaaaacgcg gagaaatttt tgaaaactgt caatatgtta gataagaaag attcttatcc
      421 ttccatgtta tccgggggac aacagcaacg ggtggcaatc gccagaggtt tagcgatgaa
      481 tcctgagatg ctgctgtttg atgaacctac ttctgctttg gatccggaaa tgattggtga
      541 tgtattagac gttatgaaaa aattggctca tgacggtatg tcaatgattg tcgtgacaca
      601 tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc ttcatggctg atggagaagt
      661 attggaagac agcagaaatg tgagagactt tttcgaaaat ccaaatgaag tccgagcaca
      721 gcaatttatc agtaaagtta tcaatcattg ataggaggaa aaagatgcgc aggttaaaaa
      781 aaatacgcct gattttcctt ttaggcattg cttttcttgc actagccgga tgtaaaggaa
      841 atgggttggc aaatgaagat attttagagc gaagcaaatc taccaatgaa atcatctggg
      901 gtgtcaagta tgatactcga ttatttggaa tgatggatat tgagagcaga actgtacaag
      961 gatttgatgt agatattgca aaagcaatca ctaaaaagat ccttggtgac aatggaaaaa
     1021 cagaatttgt tgaagtcact tctaaaactc ggattccatt attgaaaaac ggaaatatcg
     1081 atgcaattat tgcaacgatg acgatcacag atgaacgaaa aaaacaggtt gacttttcag
     1141 atgtctattt cgatgctggc caagctttgt tggtaaagaa aggaagccaa atcaaaagtg
     1201 tagatgattt gaatgcttct acaacggttc tagcagtcaa aggttctaca tcggctgcga
     1261 atatccgaca gcatgcacca gatgcaaaaa ttttagaatt agaaaactat gcagaagcat
     1321 ttactgcttt acaatctggt cagggagacg cgatgactac ggataatgcg attttgttag
     1381 ggattgcaga tgaaaatcca gagtatgagt tagttggtgg aacgtttact aatgagcctt
     1441 acgggattgc gatcaacaaa gggcaagaaa acttcttgaa agctgttaac caagcgctag
     1501 aggaaatgca tgcagatggc acatacgata aaatctatca aaaatggttt ccaaatgaaa
     1561 ctgaaggaaa agtagagtaa gggggagagg caatgagcga attgatacaa acttatggac
     1621 caatctttgt cgacgggttc aagatcacat tgttttcgag tgtcttagca cttgtgttta
     1681 gtttgattat cggaacgtta atggcaatct tccaattatc aaagaataaa gtagtgagcg
     1741 ccttagcaaa ggcatatgta gaatttttta gaaacattcc attattaatc atcgtgatgt
     1801 ttttttatgt tgtcgtaccg atttattggt ttaatctaga tggattccaa gcaggtacga
     1861 ttggattaac gatctataca tctgctttta ttgctgaaac agtacgttca gggattcaaa
     1921 ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg ttttacctat tcagaaatca
     1981 tgcgttatat tgttttaccg caagcattca aaatcgttat cccgccatta ggcaaccaat
     2041 ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt agctggttta gatttgatgt
     2101 atcaaggaga cttgatcgct agtgaaacat tcaatacctt cgatacgtat atcatcgttg
     2161 ggttatttta cttagttatc acgctaccgt tatcttactt aatggtttat cttgagaaaa
     2221 aatgggcggt tcgttcatag aaggagggga aatacatgga ttttagcggc gctttttcat
     2281 ggatgaatat ccgtttttta ttggaaggat taaaggttac tgttgaagtt tcattgtttt
     2341 cgatcatttt tagtttcttg atcggtggtc tgacaggtgt gattcgtttt gcgaatatcc
     2401 ctttcttttc caaaatcctt ggtgtagtga ttgatattat acggaatctt ccattattat
     2461 tgatcatttt cttcacttac tttgccttac ctcaaatcgg cattcagatg aacatcttct
     2521 ggtccgctgt cgcagcatta acgatttttg agtcagctat gttgtcggaa attttccgag
     2581 ctggactcaa tgctgttcca aaaggacaga tggaagcagg attatcaacg ggactgactt
     2641 atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt taaatcaatg atcccggcaa
     2701 ttgtgagcca attgatttca ctgatcaaag atacttcatt agcagtaatc atttctttac
     2761 ctgaactttc tcatcaggca cgtattattt atggacagaa caccaattat gtgcttccga
     2821 tgttcatcat catgacactt atgtacttcg tggtatgtta cgcgctttct ctgctttccg
     2881 gttacttgga aaagagacgg tacagttatt aatagatgat tcttttagcg gttgtggcaa
     2941 actaaagtca caaccgcttt tcctctttcc attcgaaaaa aagagatttc gattgtttta
     3001 agcaagtaag agcacttgaa aattcacgct aaaaagtaca caatagggag gatagaattt
     3061 attgaaaaaa aggatgatac gattgacaga taatcgccct atcggattta ttgattcagg
     3121 tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa ttaccgaatg aaaatatttt
     3181 atttgtagga gacacagcac gctgcccata tggccctaga cccgcggaac aggtaatcca
     3241 gtatacttgg gaaatgacgg attatctggt ggagcaagga atcaagatgc tggtgatcgc
     3301 ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa gctgctcttt ctattccagt
     3361 catcggtgtg atccttcccg gtactagagc ggcagtaaaa aaaacacaaa ataaacaagt
     3421 tggcattatc ggtacgattg gtacggtaaa aagtcaagct tatgaaaaag cactgaaaga
     3481 gaaagtacca gaattgactg tgacaagtct tgcttgtccc aaatttgttt cagttgtcga
     3541 aagtaatgaa taccattcat cggtggcgaa aaaaattgtg gcagaaacat tagctccttt
     3601 aaccactaaa aaaatcgata cattgatttt gggatgcacc cattatccat tattacgccc
     3661 catcattcaa aatgtaatgg gagaaaatgt tcaactgatc gattctggag cagaaacagt
     3721 aggtgaagta tctatgctgt tagattattt caatttgagc aattcaccgc aaaatggtcg
     3781 gacattatgc cagttttata caactggctc tgccaaactt ttcgaggaaa tagctgaaga
     3841 ctggcttgga atcggacact taaatgtaga acatatcgaa ttgggaggaa aataaggatg
     3901 cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg agcttcaaac aaatgttttc
     3961 aaatatccag aaggatcggt agtgatccgt tttggtgata caacagttat ttgttctgca
     4021 acaatagaaa attctgttcc cccttttttg agagaaacag gaacaggatg ggtgacagca
     4081 gaatatagta tgctgcctcg agcaacgagt acgagaaatc gtagagaaag cagcaaaggg
     4141 aaactttctg gtagaacaat ggaaatccag cgtcttattg gtcgttcctt gcgtgcagta
     4201 gtagacttgg aaaaattagg agaacgtagt atcatcgtag actgcgatgt gatccaagcg
     4261 gatggcggta ctagaacagc aagtatcact ggggcatttg tggcattgaa attagccatt
     4321 gaaaaacttt taagagaaaa agaattgtca gaagatccaa taaaagaaca tcttgctgca
     4381 gttagcgtag gaatcttacc agatgggact tgcgtcacag atttggatta ccaagaagat
     4441 tccgcagcac ttgtagacat gaatcttgta atgacagaat ctggaaaatt cgtggagatt
     4501 caaggaactg gagaagaagc aacgtttgat ggtgaacagt tgaatgaaat gctctttttt
     4561 ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac atgctttgct aacagagttt
     4621 gctcaaaatg atgagcggat agaagaaaca aaaacaatca tcattgcgac aagaaatcca
     4681 ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg gatatcatgt aaaaacatta
     4741 cttgactatc ctgaattgcc agatgtggaa gaaactggca gcacatttga agaaaatgcc
     4801 cgattaaaag cagaaacgat tgcacaattg cttgatcagc cagtcttagc agatgattca
     4861 ggtttgaaag tggatgcttt aggcggtatg ccaggaatct attctgcacg ttttgcaggg
     4921 gaacaaaaaa gtgatgcggg gaataacgcc aaattattat atgaattgac agatgtgccg
     4981 gatgaaaaaa gaacagcaca attccattgt acgttagtat tcgcggcacc caaaaaagac
     5041 agccttgtag tagaagcgga atggcccggt cgagtagccc gtataccatc tggcgaaaat
     5101 ggctttgggt atgatccttt atttatccca gaaggtaaga aacaaacagc agcagaatta
     5161 tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag caatgaaaaa attgagtgcc
     5221 gagtggaaac aatggctgga aggagagaga tagatgcggt atttagtagt aagtgataat
     5281 catggagaca gagatatcct cgttgactta gtagatcgtt atcgttctga agtggatatc
     5341 tttttccatt gtggtgattc ggaattagaa gcttctgacc cattatggga tacattccaa
     5401 gtggttcaag gtaattgtga ttatggacca ggattcgaac agaaaaaagt gatccaaact
     5461 ggtcaggata cagtattcat gactcacggt catttatcga atgtccgatt tggtttgaca
     5521 cagttagcaa tcgaagctcg agctgcaact gccgatatgg tgttttttgg tcatacccat
     5581 caaataggct gtgagatgga tcagaaaatc ctgtttttga atcctggtag catttctcag
     5641 cctagaggcc cgatccaaat cccatcatat gctcttatcg aaagtttaga tgaccaaatt
     5701 tctgttcaat actataaccg tgcccatcag gcagttgaag agttggcctt ttctttttcg
     5761 agagagcgct gaataaaaaa actctaattt ttaataaaaa taagctttat tttatgaatt
     5821 ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat agaaatggag gccatcagat
     5881 gattggacct atagtaagag aaatgctgct ggaaaatcag gaaacgtttt tgattcctgc
     5941 agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc catggtttac ttgtgttatc
     6001 gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat gatcactttg taggactagt
     6061 aagtttatca gatgtggtca ataaaatgat tgacttgcaa acaatcagca tggacccgtt
     6121 agaaggacta acggttgctg atgtgacaga aacagatgtt ccgacaattg atgaaaattg
     6181 ggaactagaa gaagtactcc atttgttagt cgattcgcct tttttaccgg ttgtcacaga
     6241 agatcggatt ttcaaaggaa tcattacaag aaaagagctg cttaaagccg taaacttcat
     6301 ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa atcgaagaat taaaagaaca
     6361 aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata atcaaaaaat aagtagaaaa
     6421 aacagcgtac cagttttgac tttatcttga aaaagaggct gggacaaaag cgtttaactc
     6481 caagaaataa gaaggaattc acgaaaattg cttttcaaat ttttgtgaat ttcagcttat
     6541 ttccgaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg ggacaaaagt
     6601 gttctttact tttgtcccac gctcttttta gtgagattcg ttgcatcgtt tgtccaaatt
     6661 attttccgac agcaattgga ctgttcggaa tcgtgaaacc agaagctgtc aattctttga
     6721 cataggagct caagaactct tcttttaagg catattgttt tccgttcaat gcgtagcaga
     6781 ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa tccaaagata gaaggttcag
     6841 tttggagctc ttcttggtat ttttcagcta atgtacgatt tactcgatca atgatctcat
     6901 agattttgtc atatccttct tcaggaacaa tccgaatatc taaaagtact tgcatatttg
     6961 cacgagagag attgctgatc gtggtgatgt ttcgattcgg gatgaaatgg acagtaccat
     7021 ccgttgcttt cagctgaagg gtacggatac caacagatac gacggttcct tcaatcgtta
     7081 agtttgctag cttgatgtag tccccaacat ccatctgttg ttccatgata ataaaaaatc
     7141 cggtaataac atcactcatg aacccttggg caccaagacc gattgccaca cctgcaattc
     7201 cggcaccagc caaaagtgat ccgatcggca caccgatagc agataaaatt gcatagataa
     7261 aaaagaaacc aagggtataa tgaaaaatag tatttaaaag actatgtaaa gtctttaagc
     7321 ggctctcgtt tagaatggct tttttagaat actgattaaa agacttatca atcagatatt
     7381 ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa aatcagatat aaactttttt
     7441 ggataaaggc agccaaaatg gcgtcccaat caatcccgtc ccaaaaccgc tgaaacgcat
     7501 ttactttgct gacagcttga tctgtcaatg tctcgccaga agagtctgtc gtaccggcag
     7561 ttgctaataa tgaaaacata aaatcctctt ttctaaaatt tgattctttc tgtattgtac
     7621 caagaagttt tcaaaaaagg aaatagcaaa aataactata atgaaggaaa aaagctgaaa
     7681 ataaaatgac aaataatatg gataatgata aaatgtggag aaaataacga aggtggtaag
     7741 tatatgcaaa tcgattggaa caatctaggt ttttcttata tcaaaactcc ttggcgtttt
     7801 attgccaaat ggaaagacgg acaatgggaa gaaggagaat tgacagaaga taattacctg
     7861 acgattcatg aaggctctcc agcgcttcat tatggtcagc aatgttttga aggtctaaaa
     7921 gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac ctgaacaaaa tagtcgacgg
     7981 atgaatcaaa gtgctagacg attgctgatg ccaatggtcc ctgaaaagat gttcgttgat
     8041 gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc cgccctatgg aacaggcgct
     8101 acattatact tgcgcccttt actgattggg gtcggtgaaa acattggtgt ccatccagct
     8161 ccagaatatt tatttgtcgt cttctgtacg ccagtaggag cgtatttcaa aggtggacta
     8221 aaaccaacga attttattgt atctgattac gatcgtgctg ctcctaaggg gactggtgca
     8281 gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag gaacagaagc aaaagaactg
     8341 gattactcag attgtgttta tcttgatcca gccacacata cgaaaatcga agaagtaggt
     8401 gcagcaaatt tctttggaat tacaaaagac ggaaaattca tcactccgca atcgccctct
     8461 atcttaccaa gtatcacgaa atattccttg ttgcagttag ctgaagaacg actaggactg
     8521 caagcaattg aaggagatat tttcattgat gatctagatc aatttgcaga agctggtgca
     8581 tgtggtacag cggctgttat ttctccaatc ggtgggattt atcacaataa caatcttcat
     8641 gtattttata gtgaaacgga agtaggacca gtaacaaaac ggttgtatga tgaattgaca
     8701 ggtattcaat ttggagacat agaagcacca gaaggctgga ttgaagtcgt ggaataattt
     8761 caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca ggaagagact ttaaaaagtt
     8821 ttttcctgat ttttatatgt tttactttaa agaatgcaac aaaagtttat gaaagatgga
     8881 cacctttgtc aacgtagata atatcaccga tgattccgct ggaaagtggg ctgattaaga
     8941 aagcacaagc attaccgact tcttcgatcg tcacaccttg ctgatcaggc gtacgagatt
     9001 cagataactg gatcagtttt tgatagtctt taacacctgt gacagctaat gttttgattg
     9061 ccccagctga gattccattg acccgaattc ctttaggcga gaactcagct gccaaataac
     9121 ggatagcagc ttcaagagaa gctttcgcaa tacccatcat gttataattc gggattgcgc
     9181 gttctgaacc tagataagtc atgctaacga tcccgctgtt tggtgcaaga aacggttgtg
     9241 cgtattttga tacagcaatt aaagagtagc tgctgatatc ttgagctaat tgatagccgt
     9301 ctctggaaat atcagacaca tttccggaca attcttcttt gttggcgtaa gcaactgcat
     9361 ggacaaggcc gtggatctcc ccagcatatt caccgatggc attcattgct ttttcgatgc
     9421 tttcgtcgct agaaacatca cattctacag tgaatgcttc ttcgccgacc aatttgacta
     9481 atgatttttt cattcgttca ttttggtaag tatagataat ttctgcccct tgtttcaaca
     9541 ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc gacgcccata atgacgattt
     9601 tttttccttc tagaaatgac ataatgactc tcctttgttt ctgataaaaa tttgatagtc
     9661 gtaaaaaaat agagaataat agctaaaata acttcttgat ttcttaatta ttagtcaaaa
     9721 tgttttgatt gtcaaactat tttttcttaa attaaaaaaa gattgctaaa tatttaggaa
     9781 tatgatactt tgatagtcaa agtaatgaaa atggcaattg cctgaaagaa ggagtttaat
     9841 tatgtcttac gtattatctg ctactgaagt aatggaattg attcctaatc gttatcctat
     9901 ttgttatatt gattatgtag atactttaga accaggaaaa aagatcgttg cgacaaaaaa
     9961 tgtaacaatc aatgaagatt ttttccaagg tcactttcct gacaatccag tgatgccggg
    10021 tgcgttgatt ctagaaacac tggcacaagc aggttcgata cttatcctaa aatcagaaga
    10081 gtttctagga aaaagagctt atatcggtgg gatcaataag gccaaattcc gtcaaaaagt
    10141 tgttccagga gatgtgctga aatgtcattt tgaaatcacc aaaatgaagg gatcagtcgg
    10201 tacagcgtta tctgcagcat atgtagatga taaaaaagtc tgtgagtgcg agttcacgtt
    10261 catcgtcaac caaaatgctt gactataaaa cctgatatag caaaacaaaa ggaagcttac
    10321 ttgcttttta tgtaaaaaga agacgcctga atcctagcat acgttgtcat ctatgcagga
    10381 ttcagacgtt atttttttat ttttgttttg cttcttgcag tatattgatt ttatcgattt
    10441 ttacttcttc ttttggtttg tctgaactat ctgtttcgat atttgcaata gcatccacga
    10501 catccattcc ttcaatcact tggccgaaga cagtataatg gccatctagt gaaggataac
    10561 cgccgttttt ataagcgtcg atgattgctt gaggatagtc gtcttttaaa agaccatcat
    10621 gcatatcatc tgtattttga acgataaaga actggctgcc gttactgtta ggatcttgtg
    10681 agcgagccat agataacgca cctcttatat tataaaggtg gttgttgatc tctgtttcaa
    10741 atccttctcc ccaaatgctt tcccctccag tgccatcgcc tttaggatcg ccaccttgga
    10801 tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt gtagtaaccg tcttttgcat
    10861 gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg aaataatttg attttgattg
    10921 tgcctttatt cgtcaccatt tcgaccagat cttcattttc ggcgacatct ttcgtcaatt
    10981 gtggtaattc caatgcgttc aaatcgacgc tactggcagt cgtgctttcc gcagtagatg
    11041 tactttttga actttctgta gacgaactag aagaagtcgt gttgtttgca gaacaaccag
    11101 cagcaaacag caatgctgct gctaaactaa tacttaaaat aactttattt ttcttcataa
    11161 ctgttccttt ccaatttact attttgttat ctttaatgat aacaaaatag agggaaaata
    11221 cctacttaaa ttattggaat ttatcattta ttttaaaaga aaaagaaaag gtgtagacca
    11281 tttactttgt gtttgctata atgtaagtgg atacaaaaac gaaaagatgc aggaggaaat
    11341 ggtaatatgg gaaaattagg aatctcgatt tatccagaac ggtcaacatt tgaaaaggac
    11401 aaagcgtact tggatttagc tcataagtat gggtataaac gggtttttac cagtttgctg
    11461 cagatcaatg atgataaaga aaaagttctt tcagaattta aagaagtagt tgattacgcg
    11521 aatagcctag gcatggaagt aatggttgat atcaatccag cgttatttga acagttggag
    11581 atttcttacg atgacttatc ttttttccat aagatgggtg cctacggggt ccgcttagat
    11641 atcggattca caggagcaga agaagcaaaa atgacgcgca atccttttgg tatcaaaatc
    11701 gagatcaata tgagttcggg cacgaattac gttgacaaca tcatgagtta ttcgccaaac
    11761 acggataatt tattaggttc tcataatttt tatcctcatc gatactctgg tttaggatac
    11821 gaacactttg ttttttgttc agaaaaattc agaaaatata atttgaatac gatggcattc
    11881 gtcaattcgc aatctgctga gtttggccct tggcctacgc aagatggctt gtgcacgctg
    11941 gaagaccacc gagatcttga aattgcgaca caagtgaagc atttaatcct tacaggattg
    12001 attgatgata tttctattgg aaatgcgtac gcaagtgaag aagaattgaa agaaatggca
    12061 gaagcattta acgcggatta tccaacattg aaagtagata cggaagaagg tattacagaa
    12121 aacgaacgaa tctgcttgtt tgataacctt catagttatc gaggggatcg ctcagaatat
    12181 atccttcgtt caacgatgac tcgtgtatac tacaaagata aagatttccc gcctcataat
    12241 acgcgagata tgcatcatgg ggatgtactg attgacaatg aagggtacgg tcaatacaaa
    12301 ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg gacgtgtcaa tgttgtagga
    12361 agaatctccg atgacgaatt attcctcctt gactttttga aaccatggtc aagctttaaa
    12421 ttaattgaaa ataaaaaata gataattagt tattgcagaa ataatccgct agaaatagcc
    12481 agttatttct gcttttttat tttgtttttt tatagg
//
LOCUS       DAFFSV010000089        12336 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFFSV010000089 DAFFSV010000000
VERSION     DAFFSV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.089"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            80..1012
                     /locus_tag="LR110_002072"
     CDS             80..1012
                     /locus_tag="LR110_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823025.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1233..2996
                     /locus_tag="LR110_002073"
     CDS             1233..2996
                     /locus_tag="LR110_002073"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBL4823026.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3080..3538
                     /locus_tag="LR110_002074"
     CDS             3080..3538
                     /locus_tag="LR110_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBL4823027.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3647..4330
                     /locus_tag="LR110_002075"
     CDS             3647..4330
                     /locus_tag="LR110_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL4823028.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4477..5091
                     /locus_tag="LR110_002076"
     CDS             4477..5091
                     /locus_tag="LR110_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBL4823029.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVLALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5107..5313
                     /locus_tag="LR110_002077"
     CDS             5107..5313
                     /locus_tag="LR110_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823030.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            5854..6984
                     /locus_tag="LR110_002078"
     CDS             5854..6984
                     /locus_tag="LR110_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL4823031.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSVA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            7076..7831
                     /locus_tag="LR110_002079"
     CDS             7076..7831
                     /locus_tag="LR110_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL4823032.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            7889..9928
                     /locus_tag="LR110_002080"
     CDS             7889..9928
                     /locus_tag="LR110_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBL4823033.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(10037..11188)
                     /locus_tag="LR110_002081"
     CDS             complement(10037..11188)
                     /locus_tag="LR110_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBL4823034.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(11342..11872)
                     /locus_tag="LR110_002082"
     CDS             complement(11342..11872)
                     /locus_tag="LR110_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL4823035.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(11971..12321)
                     /locus_tag="LR110_002083"
     CDS             complement(11971..12321)
                     /locus_tag="LR110_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBL4823036.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         4315 a         2103 c         2443 g         3475 t
ORIGIN      
        1 cttgctagct ttagtacgtt atgtcaattg ggaaggctat gaaaataaaa atccgtatgt
       61 agaagaagct tgtaacgagt tgattgatta tgtagagact cattttttta aaagaagata
      121 ttcggaagat gaaaagtcct ttatcatacg agaaatagag atcgcaatcg gaagaaaggc
      181 atttcccctt ggactgaaag aagaagataa gaaaagcgca gaaacgaagc cgttatttca
      241 attagtctgt caaggattgg aagagcaaaa aggaattcta gacttacaag aagaagatgt
      301 ctattatatt ttttctttgt tcaacagtag aaattatacc aatgagaaca tggagctgtt
      361 aagaaaagat ttcgaagttg tttataaaaa ttttgttctc aatgacctat cactgaatga
      421 attgatttta cagattatta gtatcgctga aatcgacgat gcagaaaatt ttattttcaa
      481 gcaatccttt ttacaatttg tccgtacgtt gtgggcagat ggacaagtat ttttaccgga
      541 aaggatctat cttctatcag aaaaagaaca acttctgtac ggaaaaattg tagaaatatt
      601 ggaagagtgg aaaaagaaaa atcaaatcca actaagatgg aatgaaaatt tcattcgaaa
      661 attcgtcaaa agtctttctt tgatcaacag tgaacaagcg gggagacagg aaatagaaat
      721 ttttgttgtt tcagaaagtt cagtcaaaca atttttttat cgaacccaat ttcatttgta
      781 tgcggaagaa ggcatcgctg aaataaatcc tattttattt cgttcattag aagaattgcc
      841 tgatgaatgt ctatgtgcga aaaaaagagt ggttttgtgc gatgctgcta gctatcaaaa
      901 tggattagaa actgaaaaga caaagatcta tccaatctca ttaaaaggtg caaacatcca
      961 tttgtatcat attgctcagg aacttcacct attaaaaaaa catatttctt gatgagacct
     1021 taattaggag accttctaca aagcttttta attgtctgta gaaggttttt atttgagtcc
     1081 tgattcgtgc gaagatctac ttatataaca agctttctct gaaatttttt taaaatgcta
     1141 ttgacttaga gtcaactcta agtagtagaa tagataaagt gtaacaaatc tattttcatt
     1201 aaaagaagat agtacggagg agagaatgta ttatgagtac agtattacat gtattggatg
     1261 caatcaatag ccccgcagat gtcaaaaaat tatctgttga ggaaatgaaa cagttggcag
     1321 aagagatgcg ccaaaaaatc ttgcaaaagg acagcgatgt aggtgggcac gttggtccga
     1381 atctaggtgt agtagaatta acgattgcgt ttcattatgt atttgattca ccaaaggata
     1441 aaatcgtttg ggatgtttcc catcagtctt atggtcataa gcttttaact ggtcgaaaaa
     1501 atggttttga aaaaggacat taccgtgaag tgagcggata ttccaaccaa catgaaagta
     1561 aacatgacta ttttacagtg gggcacacat ctacatctat cagcttagca acaggtttag
     1621 ctaaagcgag agatctgaag aaagaatcag gaaatgtggt agcatttatc ggtgacggct
     1681 ccctaagtgg tggcttagct tttgaaggat tgaacaatgg tggtgtttta caatccaatc
     1741 taatcgtatt agtaaacgat aatgagatgt ctattgatga gaatcatggc agtttatata
     1801 aaaacttagc tgagcttcgt caaaccaacg gtaaaagcag caataaccta tttaaagcaa
     1861 tgggatttga ttatcgttac gtggataatg gaaacgatct ggaaacggtg atccgtttat
     1921 ttgaagaagt aaaagatatt gatcatccga ttgtacttca cgtccatacg gaaaaaggtc
     1981 gcggttatca gccggcaatc gataataaaa tgaaatatca ttggcatgta ccatttgatc
     2041 tgtccaccgg ggaggcaaag caagtaactt ctggagaaac atttaatatg atcatcatgg
     2101 attatttgga taagaaaatg caaacaagta cagctcctat tgccgcagtc aatgcagcga
     2161 taccaggcgc ctttggactg aaaacattcc aagaaaaaca tccagatcat tattttgacg
     2221 tggggatcgc tgaacagcac tctatcactt tcgcttctgc tttggcttct cagggagtac
     2281 gtccagtcgt cttccaatca agtactttct tgcaacgggc atatgatcaa ttatcacatg
     2341 atttagcgat caacgagaat ccagtgatta ttttagtcgg tggcggaacg atctcagcta
     2401 gtgaccgaac acaccaagga acatttgaca ttccttactt atcaagtatc ccgaacatca
     2461 aatatcttgc tccagcaaca aaagaggata tgatctctat gctagattgg gcgttcgaac
     2521 aaacagcgca gcctgtagca atccgaattc ctacacatgg tgtagaacat ggtacagcta
     2581 tcgcaaatga ttatagtatt ccagcctatc aaacagtaaa taatggagga aaagtagcaa
     2641 ttttagcttt aggcggtttt cttgaattag gaaaagaaac agtaaaagct tttagaaaaa
     2701 aaggaatcga agcaacgttg atcaatccag gaacgatcac agaaaatgac gtagaaacat
     2761 tagataaatt aaaagaaaca cacgaattag tcatcacatt agaagatggt agtttgtcag
     2821 gaggatttgg cgaaaatatc agtcgttatt atggtgattc ttcaatgaac gtattaaatt
     2881 ttggtgcaga gaaagaattc acggatagcg ttcctgtagg gcagctttat gaacgttatc
     2941 atttaacacc aaaacaaatc gttgaagata gtctgcgtgt cttgaatatt caataattca
     3001 ttatgctcac catccagata attgtttata ctagtatctg gatggttttt tcattataaa
     3061 aagcaaaggg gtggatgtta tgtatcagca agtagaagaa tatgtcaaaa agttccaaga
     3121 aaaatatcct agttatcaat cagaaaaatt aggtttcttt tcatttgggg atacagaaaa
     3181 aatggcagac gagttgagcg aactagttgt aaaaaagata aagactggta catcaagtgg
     3241 ttacgatctg tataaacaag agaatgagcc tttaccaaaa gttggtcaat tagacgtggt
     3301 cctgaatgga aaaaatgaac cagtttgtat catcaaaaat ataaatgtga ccatcttgcc
     3361 ttttagtgaa gtcacgaaag agcatgcttg gaaagaagga gaaggggatc ggacattagc
     3421 ttattggcgg aaagtccatg ctgagttttt taaacatgct tataaagaag cgttgaacat
     3481 agaatttaca gaagagaagc tggtcgtata cgaagaattt gaagtaattt tcttttagta
     3541 agaaaggtct gctgaaagaa atagctcatt attgtctcct gaatagaaaa gggttacatt
     3601 tttatatgaa gctgtatttt tatataacat tactggagga gatattgtgg atacactgga
     3661 acaaatcaaa ggcggagtcg tcgtttcttg tcaagctttg gaaaacgagc cgctacacag
     3721 ttcttttatt atggggagaa tggctttggc tgcaaaagaa ggcggagcag ttggtattcg
     3781 agcaaatacg acagaagata tcaatgaaat caagcgaatc gttgatttac cggtaatagg
     3841 gattatcaaa agagattacg atgggtcaga agtgtttatt actgcgacag aaaaagaagt
     3901 ggaagaactt ttggagacgc aagcagaaat cattgcactg gatgcaactt ctcgtaaacg
     3961 tccgacagaa aaaacaactg ctgaacttgt ccaaatgatt cacgaacacg gtcggttagc
     4021 tatggcagat atttcgactt ttgaggaagc gcttcaagcg caagaagatg gttttgatct
     4081 tttgtctacg actttagcag gctatacaga atattcacga aagacgcaga cacctgattt
     4141 tgatttattg aatcaaatca tagaacaagt cgatatccca gtaattatgg aaggacatac
     4201 tgactctcct gaacaagtag agaaagcata tgaattaggt gctttttcag tcgtagttgg
     4261 atctattatc acccgtccgc aattgatcac taaaagatat gtcgaagccg caagtaaagc
     4321 aaataactaa aaaacacgca cggcacagat tcttgacaaa aaagaaaaat tgatagatag
     4381 tttaaagcag attctaagaa gatgaaaaaa gcattacttt tcattttgtc ttcaagatct
     4441 gctttttaac gaattttata gaaagaaggc gaaaccatgt ttgaacggag tccttcagaa
     4501 ttgacagaac gtgaaaatta caagttgctg attggttcag tcatccctag accagtagca
     4561 gtagtaacga ctaaatcagc aaaagatata gtgaatatcg cacccttcag ttacttcaat
     4621 atcgtcagtt cgaatcgacc catcttatcc ttggcgatcc agagaaaaca agatgcagta
     4681 aaagacacag caaagaatat tcttgagaca agagaagctg ttatccatat ccttgatgag
     4741 gacaacgtag aagaagtaaa taaaacagca gccaatctgc ctaatgagga aagcgaactg
     4801 tacctgactg acttgacttt aactgattca gtaatcattt cagtactagc attgacagaa
     4861 gcaaaagttc gttttgaaac atccttatat gaacatgtcg agatacggaa taaaaataaa
     4921 gtaacagctg atttattttt gctagaagta cagaaatatc atttaaatga aacagtctat
     4981 gatgataaga caggaaggat cgatccaaga aaattgaatg ctgtaagccg tttggcagga
     5041 aatgattatg cagcaatagg agaaattttc acgattgcac gtcccgaata aaaaggagca
     5101 ctagttatgt taaaaataag ctataaacca tcgacagatt caaaagaaat gaaaaaagaa
     5161 tatgaaacag tgaatgattt tctgcaaggg cagtacttag aagttccgcc attgcaagac
     5221 cattttgtcg ttactactgt tactttagat ggtaaggaaa tagaaatgcc agatcaaacg
     5281 attagcggat tgtttaatta ctttaacaaa taaatattta ttccgaaaag tgaaaaaaca
     5341 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
     5401 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
     5461 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
     5521 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
     5581 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
     5641 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
     5701 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
     5761 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
     5821 gagatgcggt gaaaaaagat cgattggtgg attttgggtc cttatttgac tttgtccatg
     5881 atcggtcttt tagaagtata cagtgccagt tcttatcgtt tattgcaagc cgatgaaaat
     5941 acaaaaagtc tattattgcg tcaactaatt ttcatatttt tgagttggag cgtgatcttc
     6001 ttagctcgtt cagtcaaact acactattta cttcacccta aaatagcagg atacggttta
     6061 gccttatcga ttttcttttt agtattagta agaataggga tattcggtgt cactgtcaac
     6121 ggcgcacaac gttggatctc tctgtttggc attcaattcc agccttctga actggcaaat
     6181 ctttttttga ttttttattt aagctggttt tttcgtgacg gaaatagtag cccaaaagat
     6241 ctaaaaaaac cattcctgat tacagtaggt ataacttttc tgattttatt tcagccaaag
     6301 attgctggag cattgatgat cctttcggtt gcgtgggtca tattttgggc agcggcggtt
     6361 ccatttaaaa aagggatcta tctaatcgtt actttttctg cattgctgat tggagcagca
     6421 ggcggggtat tatatttagg aaataaaggt tggcttccac agatgtttaa tcatgcttat
     6481 gaaaggatcg cgactttaag agattctttt atcgatagtc atggtgcggg ctatcaaatg
     6541 acccattcgt tttatgcact atataatggt ggcatatttg ggcggggatt aggaaatagt
     6601 atcacaaaaa aagggtatct tccagagaca gaaacagatt ttattttttc gattattaca
     6661 gaagagcttg ggctaatcgg tgcattgtgt gtgcttttct tattgttctc attatgcatg
     6721 agaatctttt gtttaagcag tcgctgtaaa aatcagcaag ctggattgtt tcttttagga
     6781 ttcggaactt tgctattcgt tcagacgata atgaacgtgg gaagtatcgc ggggctgatg
     6841 ccaatgactg gtgtgccatt gccatttgtt agctacggag gaacaagtta tctgatcctt
     6901 tctttaggaa tcggaatcac gttgaatatt tcttcaaaga tacaggccga agagttggcg
     6961 ctctatcgac ctgaaaaaca ataaagagaa actgagaaac tctcagtttg aattatcata
     7021 aagtacaatc ttttattttt caagcgagag gttcttttag aaagaggaat tcgcaatgaa
     7081 agtatctcaa aaaatcatat tatctatttt gttactgctt acattagtag ttggctattt
     7141 ttcgattgag tttatccatg gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt
     7201 agagccgaaa agtatcccta ccacactaaa tgtggctttg attggttcag atgctcgttc
     7261 gaaagacgaa aacggtcgct cggattcact tctggttgca caatacaacc agaaaacaca
     7321 acaagcaaaa ctgatctcta tcatgagaga ttcatatgtc gatattccag gttacggaat
     7381 gaataagata aatgcagctt actcttatgg gggagttgat ttattaaatc aaacattaaa
     7441 ggaaaatttc aaatttgaag ctccctatta tgcaagtatc acatttcaag attttatcga
     7501 ttgcgttaat gaactgtttc ctggtggagt caagatcgat gcagaaaaat ctctagattt
     7561 agatggcgta tatataaaca aaggagagca agtgatggat ggtaatactt tactccagta
     7621 tgctcgattt cgggaagaca aagaaggaga cttcgggagg atcagaaggc agcagcaagt
     7681 gatcaaagct atctctcagc agctgaaaga cgtaacttca atattcaagc tacccaaagc
     7741 ggttgggaaa ttacttggta gtatccaaac gaaccttcca gaaagtgtgc ttctggattg
     7801 tggtatggat ttcctcaaag acatatgtta gaataaacag gtataaatag tgaaataaag
     7861 gaatgacaag caagagaagg aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg
     7921 aacaggcgct tatattgccg gcgcagtgat cttaatagca gctgcgggtg gcggttattt
     7981 ttactaccag cactatcaag aaacccaagc agtagaagct ggagaaaaga cagttgagca
     8041 atttgtccaa gctttgaaca agggagatta taacaaagct gcagaaatga catcaaaaaa
     8101 ggcggcaaat aaaagtgcat tatctgaaaa agagatctta gataaatacc agaatatata
     8161 cggtgctgct gatgtcaaag gacttcagat atcgaatcta aaagtagata aaaaagacga
     8221 ttctacttac agtttttcat ataaagcaaa gatgaatacc tcattaggtg aattgaaaga
     8281 tctttcttat aaaggaacat tagacagaaa tgatggtcaa accacgatca actggcaacc
     8341 caacttggtt tttccagaaa tggaaggaaa tgacaaagta agcctgacca cacaagaagc
     8401 agcaagaggg aatattatag atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa
     8461 acaattagga gtcgttccaa gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa
     8521 agcgattgct tcctccttcg acttaacaga agatgctatc aatcaggcga tttcacaaag
     8581 ctgggtacaa cccgattact ttgtcccatt gaaaatcatt gatggagcaa cgccagaact
     8641 tccagctgga gctaccattc aagaagtaga cggcagatat tatcctttgg gtgaagcagc
     8701 tgctcagctg attggttacg tgggagatat cacagcagaa gatattgata agaatccaga
     8761 attaagcagt aatggaaaaa tcggtcgctc aggtttggag atggcttttg ataaggatct
     8821 tcgtgggaca acaggtggga aattaagcat tacagatgca gacggtgtag agaaaaaagt
     8881 tctgatcgaa catgaagttc aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc
     8941 acaaaaaaca gcttttgaca gtctaggagg aaaagctgga tcaactgttg cgacaacgcc
     9001 aaaaaccggt gatcttcttg cgcttgctag ctctccaagc tatgatccaa acaaaatgac
     9061 aaacgggatc tcacaagaag attacaaagc ttatgaagaa aatcctgaac atccattcat
     9121 cagccgattt gcgacaggtt atgctcctgg atctacgttt aaaatgatta cagcagcaat
     9181 cggtctcgac aacggcacta tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg
     9241 gcaaaaagac agttcttggg gatcttatca agcaacgcgt gtcagtagtg atgtatcaca
     9301 agtagactta aaaactgctt tgatctattc cgataatata tatgcggcac aagaaacgtt
     9361 gaaaatgggt gagaaaaaat ttcgtacagg cttagataaa ttcatttttg gtgaagacct
     9421 tgatttgcca attagtatga atccagcaca aatttctaat gaagatagct ttaactcaga
     9481 tatcttgcta gctgatactg gatatggaca gggcgaactt ctaattactc ctatccagca
     9541 agcagcaatg tattctgttt ttaccaacaa tggcacactt gtctatccta aattgattgc
     9601 agataaagag acaaaagata agaagaatgt catcagcgaa acagcagtac aaacgatcgt
     9661 gccagatctg agagaagttg tgcaagatgt aaatggtaca gcacattctc tttctgcttt
     9721 agggattccg ttggcagcga aaactggtac agcggaaatc aaagaaaaac aggatgtaaa
     9781 ggggcaagag aacagtttct tgtttgcttt caatcttgat aaccaaggtt atatgatggt
     9841 cagcatgttg gaaaataaag aagatgatga ttcagcaacc aaacgagcat cggaactatt
     9901 gcaatacctc aaccaaaatt atcaataaaa aattgctttt taagtacagt aaaaggactg
     9961 tggcgttatc gtcacagtcc ttttttcaaa taaacggttt tcacgatgtc tctttcacaa
    10021 cttctttttg atttactcac acttcataaa ctttttttgt aaaatatttg agaacaagtg
    10081 gagaaataag tgtacttaat aaaatcacga taactaaagg tgaatagtac tgtggttcga
    10141 tcaattgact ttgctgtcca atttgtaaaa tgatcaaagc catttctcca cgcgaaatca
    10201 taccagcacc gaccatcaat gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat
    10261 aacctccaag caatttggtc aaaatcgcta caagcgtcaa tatagaaata aagagtagtt
    10321 gttcactaaa gtcagaaaaa tcaacttcta atcctacact gacaaaaaat actggtataa
    10381 atactgcata tcctaaagcc tctacattgt ggtatacttc ttctttaacc tttgtctgac
    10441 tgacagctat cccagcaaaa aaagcaccaa tgacagagct aagcccaaca agatccgcta
    10501 cgtaagacat tcctaaacaa ataactagcg atacgatgat aatagaagaa tttgcgtaca
    10561 ctttttctgc aaatgaaagt aggaaaggcg caatccattt gactaataag aaaataaaaa
    10621 caaaataaat tagctgttca atcaaaacaa gcggtaaaga agcattgcta gaagcttccc
    10681 ccgttagaaa agataggctg aaacttagaa ctaaaacgac taaaatgtca tctaccacag
    10741 aagcacccaa aattgttgat ccttcttttg tattcactac gtttagttct tttaatactt
    10801 ctactgaaat actgacagaa gttgccgcaa gaataatccc aaagaaaatc gcttcatttc
    10861 ccgaaacttg gaatgctttg cctgtcaacc agccaaaaac aaccgggaag atgatcccaa
    10921 gcaaagcaac gaacatacca ggtcggaaat attttttcaa aagagataaa tcactttcca
    10981 gaccagctaa aaacattaat aagatcacac caatctccga aaagtcatgg actagaatat
    11041 caggtttgac ccaattcaat ccagcttggc ctagcaaaac accaacaagt aattgtccaa
    11101 tcactgctgg gatacctaat ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata
    11161 tcaggcataa aataccaata aattccattc tcatcattcc tttgtataaa atatagcccg
    11221 aacttagcat aacatacatt attgaaaaaa taggaaaata tactcacaaa aaaaccaaca
    11281 atccaaaaga tttttttgga ttgttggttt ttatacggtc tattttaata actaaaaatg
    11341 attaacgtat agtcattttt cttattctga tgaaattgat aatctcctgg atgcccatga
    11401 aaatcaaaat aaagccgaat atttggaata gaagcatcaa acttttgaat ggattgaaca
    11461 ataagacgaa tccgccaatc aacaacagaa tactactata aacactccaa cttttagctt
    11521 ttgtattcat gctgaagaac aactggaaca agctgttgat gataacagct aatccaagaa
    11581 gaatcggcag gatagaaacg attgcaggtg caaaagcata gacgatcaga gctaagatca
    11641 ggaaaaaaag accactgaac agaccgatac tccaagaacc tgttttcctt ttaaaacggt
    11701 aagcctcaaa aagattcaac aacccaagca aaacaagata ggcggtgatc agataaccga
    11761 taaaatgaaa aacagcagaa gggtttagca caatcgcaat cccagaaata atataaacag
    11821 cagctcttaa taaggcgtat ttttgaatat tttgtttaat cgtctcaaac atataagaaa
    11881 acctcctttg acttatttta acgtgaacta gtcattttac caaattaaat gttacaaaat
    11941 gagcgaagga gaagaatatc tctggaaaaa tcaacttctg aggatctttt ccattttttt
    12001 gccttttgct agctcatcga tcattttatc taaataacga atttctttca tcaagttatc
    12061 ctcgatgtct tctactcgta caccacaaac aacacctgta atcaaggaac gtgaaggatt
    12121 ggggacaggg gcttccgcaa aaaattgttc gaaagtcact tgtttttgta gttgatattc
    12181 tagttctgtt tgggaataac ccgtcagcca acaaatgatt tcatctactt cctcttttat
    12241 acgatctttt ctctccgctt ttgctacata cataggataa acgcttgcaa aacttgtcgt
    12301 aaaaatacga ttttgcgcca taatcagtca cctttc
//
LOCUS       DAFFSV010000090        12321 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFFSV010000090 DAFFSV010000000
VERSION     DAFFSV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.090"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            122..577
                     /locus_tag="LR110_002084"
     CDS             122..577
                     /locus_tag="LR110_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL4823037.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            918..1520
                     /locus_tag="LR110_002085"
     CDS             918..1520
                     /locus_tag="LR110_002085"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823038.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            1614..1946
                     /locus_tag="LR110_002086"
     CDS             1614..1946
                     /locus_tag="LR110_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HBL4823039.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(2097..3076)
                     /locus_tag="LR110_002087"
                     /pseudo
     CDS             complement(2097..3076)
                     /locus_tag="LR110_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3081..3794)
                     /locus_tag="LR110_002088"
     CDS             complement(3081..3794)
                     /locus_tag="LR110_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL4823040.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVSANA
                     VTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYDFFNFYNLDQLDTIITDNQ
                     ISPQHLEEFSQYTTILKAD"
     gene            4284..4709
                     /gene="lacA"
                     /locus_tag="LR110_002089"
     CDS             4284..4709
                     /gene="lacA"
                     /locus_tag="LR110_002089"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HBL4823041.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            4726..5241
                     /gene="lacB"
                     /locus_tag="LR110_002090"
     CDS             4726..5241
                     /gene="lacB"
                     /locus_tag="LR110_002090"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HBL4823042.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            5252..6184
                     /gene="lacC"
                     /locus_tag="LR110_002091"
     CDS             5252..6184
                     /gene="lacC"
                     /locus_tag="LR110_002091"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HBL4823043.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            6187..7167
                     /gene="lacD"
                     /locus_tag="LR110_002092"
     CDS             6187..7167
                     /gene="lacD"
                     /locus_tag="LR110_002092"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL4823044.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            7195..7512
                     /locus_tag="LR110_002093"
     CDS             7195..7512
                     /locus_tag="LR110_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL4823045.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            7512..9218
                     /locus_tag="LR110_002094"
     CDS             7512..9218
                     /locus_tag="LR110_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4823046.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTLDGQGALAFVQQQ
                     FD"
     gene            9360..10766
                     /gene="lacG"
                     /locus_tag="LR110_002095"
     CDS             9360..10766
                     /gene="lacG"
                     /locus_tag="LR110_002095"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL4823047.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            10789..11061
                     /locus_tag="LR110_002096"
     CDS             10789..11061
                     /locus_tag="LR110_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HBL4823048.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            11082..11981
                     /locus_tag="LR110_002097"
     CDS             11082..11981
                     /locus_tag="LR110_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBL4823049.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3951 a         2085 c         2441 g         3844 t
ORIGIN      
        1 ttgttaagtg atataataaa ccataaaaga agtttagaaa tacggtcctg gtcatcaata
       61 aataaacaat ttaattaatt atttatttaa ttatttaatt tcagaaaggt atacatgaat
      121 catggtgcaa caaattgtcc tacccatcaa agactccagc gtcttaaaga tggtacaaga
      181 tacacttctc gatagtttcc gtgctggtcg ccgaaattac accgtttttc aagttggtaa
      241 ggccacgcta ctacgtgtga gtgatgtcat gacattaaaa aaatcagatg tctataatcc
      301 agacggctct gtcaaaaatg caacctttat tcatgataaa aagaccggta aagcaaacac
      361 gttatattta aagcctgttc agcaagactt gttgcaatat catgattggc tggcccaaga
      421 aaatatcaat tctgagtggt tatttccctc gacagcccat tatgatcttc atatcactga
      481 gaagcagttt cataaggtga tggcgcatgt tggtgatctc ttaggaatca acttggaaga
      541 caaagagaaa aaattgcgag tcagaaaacc gttttgagat atgaacagca tttgccttca
      601 cgccttagcg attttaaata atagccttga aagtgattat ggaaaagccc tctacgctca
      661 atgcaaaatt gacgtagaaa ctatttttga tcgattgaaa ggtgtatttg gtatgtgaag
      721 aacccatgtc agaggcaaga acgttgcacc tactgacatt ggaatgatgc ttatgagcat
      781 gaatctaacc aaattatccc ttgaggctag aagaaaagcg gaagcttttc aacacaaagc
      841 agccaaaaat aaaaaacgcg acgaagcaat cacatttatg attattcatc gcgtttttta
      901 tatttagagc tagttatttg ctagacccac tatttacaga tgaaacgttt atcacctttg
      961 gttttgggca tttagtggaa ttagcagaac caggtcatta tgacgaaaag tggcaaaatt
     1021 ggaaacttga atctttgccg atttttcctg atcgatacga ttttgaagtt gcaaaagata
     1081 agggaaagca gtttaaaatt gttgcagaac ttctcaaaaa ggcaaataca attattgttg
     1141 caacagatag cgacagagaa ggtgaaaata tcgcctggtc gattatccat aatgcaaatg
     1201 ccttttcaaa agataaaaca tttaaaagac tatggatcaa tagcttagaa aaagatgtga
     1261 tccgaagcgg ttttcaaaat ttgcaaccag gaatgaatta ctatcccttt tatcaagaag
     1321 cgcaaacacg ccaaattgcc gattggttga tcggcatgaa cgcaagccct ttgtatacgt
     1381 taaatttaca acagaagggc gtacaaggta cattttcact aggacgtgtt caaacgccca
     1441 ccttatacct tatttttcag cgccaggaag ccatagagaa ttttaaaaaa gaaccttttt
     1501 tcgaggtgga agctaagtaa aacaagggtc atttaagggc gttctaagcc ccacacagcg
     1561 ttttaaaacc caagaggagc tttttagctt ttgtttcttc taaacaagct aaaataggca
     1621 atcaagaggg gataattgct gatgttcaaa ccaaagagaa gaaaacgaat agtccgagtt
     1681 cgttttcttt aagtagtttg caatcaaaag tcaatcagct ttataaagcg acagcgagcc
     1741 aaactttaaa agctatgcaa ggactgtatg aagcaaaatt attgagttat ccaagaacag
     1801 atacaccatt tattacagag aacgaatttg cttatttaaa agcgaatttt ggcaaatata
     1861 gcggtttttt aggacttgat cttgaaatgt tgttatcatt gataatgata cgttcaaaca
     1921 acagcaccca aattttgacg aattagtgaa actctatgaa aaagacgtag taaaacatgc
     1981 aacatcctat tctaatcagc tggtaaagct taattaggtt atataagctt gaaaataaag
     2041 ggtttgtcca atatggacaa gcctttttct tatgttttgc cacagatttg ccacacttat
     2101 tgtgtaagag tatcaaggat aggtataatt tgatactttg tttttttagt gatgtgcaaa
     2161 taaatttctg ttacacgact acctctagaa tgccctacac ggtcttgaat tgcttctagg
     2221 ggtattcctt actctgctaa gaaactaata tgcgtatgac ggaatatatg ggtcgtaata
     2281 tgtttattaa aactaagttt attatcaata tcaattcctg ctcgttttgg attacctttt
     2341 cgatttaatt ttccattttc aggtgagttg aggaaattgt taaggacact ataatgaaga
     2401 ggtgttccat tatagtcggg tttatgacga cttttggaag taaaaatcca tccattatct
     2461 ttataatcag gattgaatct catatgttgg tgatttaact caatttcctc tttaagaatt
     2521 tctatagccc ttttagataa ttggacattt cggtaacttt gtaagttttt tacattacct
     2581 tcatagctat tttctgcagc tcctccgtga gtggactgac taatgatgat attttgcttt
     2641 tcaaaatatt gataggcacc aatattttgg agtttcgact ctgtaagtca acagggccat
     2701 tagttagaat acctattggg atgcggtttt tatgtagagc cagcagtagt tgatgtatct
     2761 caggaatcat cgtaatctga tcgagttcat attcatagtc agcttggaag tctagagctt
     2821 ctgtcctaga aatactatcg atgccaaatt cttccatagt cttctgacag cgaaaaattc
     2881 taagctgctc aatactgatg agttttttcg tgtatttagg aaaagcaaca tcagaccaat
     2941 atctgaaacg tttgtaaatg ttatctattt gttggatatc aatcgttgga aaacatttct
     3001 caacgctacg tttaaagggg agttgatggt cataaagagt gtcatcaagg tcaaaaacaa
     3061 tatttttata agtcataatt ctagtccgct tttaaaatag tagtgtactg gctaaattcc
     3121 tctaagtgtt gagggctaat ctggttatct gtaatgatgg tatcgagttg atctagatta
     3181 tagaagttaa agaaatcgta tcgatcgaat ttagtactgt ctactaataa gaatttttct
     3241 acagcattgt ttagggcaag ttgttgaatc acaccttcct tgtcactata tgtagcaata
     3301 gaattatggg taacagcatt tgcactaaca aaagctccag ttatttctct atattcaccg
     3361 ccaagaagca ataaatcaat ggtttcgcta tcatttagaa tcaagaacac agggagacta
     3421 tttgtaatga cacgaatttt ataaccttta cgacctttca attctaatgc cagttgttca
     3481 agtgtagttc caggtccaat aaaaatagta tcgccatctt tgattaaaga gcaggctttt
     3541 ttagcaatgt caattttttc ttttgtattt agacttttct tctcgatatg tgtcttttca
     3601 agtggctttt tagaggaaag caattgtgca ccaccatgag tacgtgttaa aagtccatca
     3661 gcttctagag catcaaggtc tctacgggct gtcatatcgg aaatatctag ttcatctatt
     3721 atttctttta tggttatggt cccatcaatc tttaacatat ctaaaatttt ttctaatcgt
     3781 cgttttttgt tcatatgaag acccttcttt cataaagtaa tttttttcca aagataattc
     3841 tcttttaatt gtatcataaa agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa
     3901 aacaaatgtt agatgatgaa ataagaacag aggattgacg tatattagct taggtcagat
     3961 tttgtataag acgaaaataa agtaggacct cttaatcagt aagttataga aagtaaaaga
     4021 cttttgtaat acttgaatag atatttcacg tccattttgt gatggattaa atgaacaaaa
     4081 atgaacaata atttaacggt gttatctatt ttttaaaaaa acaaataaaa aaaacaaaaa
     4141 ataaacaaaa aaagtgttgc tttttgtttg aatgcttgat attatataaa caaagaaatg
     4201 atgaaaacgt tttttcgaac attttgcaaa atgttttcta cttctacgta gcatttcttt
     4261 ctaaaattta ggaggtggtc aaaatggcta ttgttgttgg tgcagatctc aaaggtacga
     4321 gattaaaaga tgttgtgaaa aattttcttg tagaagaagg ttttgaggta attgatgtga
     4381 ctaaggacgg acaagatttt gttgatgtta ccttggcagt tgcctctgaa gtaaataaag
     4441 atgagcaaaa cttaggtatt gtaattgatg cttatggagc tggtccattc atggtagcaa
     4501 ctaaaattaa aggtatggtt gcagcagaag tttcagatga acgctcagct tatatgacac
     4561 gtggacataa caattcacga atgattacgg ttggcgctga aatcgtcggt gatgagcttg
     4621 ctaaaaacat cgctaaaggt ttcgtcaatg gtaaatatga tggcggacgt catcaaatcc
     4681 gagtagatat gcttaacaaa atgtgttaag aaaaggagtt aaggaatgag aatcgcaatt
     4741 ggatgtgacc acattgtcac agatgaaaaa atggcagtat cagaattctt gaaatcaaaa
     4801 ggacatgagg tccttgattt cgggacatat gaccacgcac gtactcacta ccctatctat
     4861 ggtaaaaaag tcggtgaagc agtagtaagt ggtcaagcag acttgggagt atgtatctgt
     4921 ggtacaggtg tcggcattaa caatgcagtc aataaagttc caggtgttcg ctcagcgttg
     4981 gttcgtgata tgacatcagc tatatatgca aaagaagagt taaatgcgaa tgttatcggt
     5041 ttcggtggaa agattactgg tggtcttctt atgaatgaca tcattgaagc tttcattgaa
     5101 gctgagtaca aaccaacaga agaaaataaa aaattgattg caaaaatcga acacgttgaa
     5161 acacataatg cacatcaagc agatgaagat ttctttacag aattccttga aagatgggac
     5221 cgtggtgact accacgatta aggtataaca aatgattctg acagtcacac taaatccttc
     5281 agtagacatc tcttatcctc tagaaacatt gaaaattgat acagtgaata gggtgaaaga
     5341 tgttagtaag acagctggtg gaaaaggctt aaatgttact agagtcttgt atgaatctgg
     5401 tgataaagtt actgctacag gtttcttagg tggtaaaatt ggtgaattta ttgaaagcga
     5461 attagaacaa tctcctgtta gtccagcttt ttataaaatc tcaggaaaca ctagaaactg
     5521 tattgctatc ttacacgagg gtaaccaaac agaaattttg gaacaaggac caacaatttc
     5581 tcatgaagaa gcagaaggct ttcttgacca ctatagcaat cttattaaac aatcagaagt
     5641 tgtgacaatt tcaggaagtt taccttcagg acttcccaac gattattatg aaaaacttat
     5701 ccaacttgct tcggatgaag gagtagcagt cgttttggat tgttcaggtg cacctcttga
     5761 aacagttcta aaatcgtcgg ctaaacctac tgccatcaaa cctaataatg aggaactttc
     5821 acagttgctt ggaaaagaag taacaaaaga tattgaagaa ctcaaagacg ttcttaaaga
     5881 gtcacttttt agtggtatcg aatggatagt ggtctcatta ggtcgaaacg gtgcctttgc
     5941 aaaacatggt gatgttttct ataaggtaga tattcctgat atcccagttg ttaatccagt
     6001 cggatcaggg gactcaacgg tagctggtat tgcatcagct ttaaatagta aaaaaagtga
     6061 cgctgactta ttaaaacatg cgatgacatt gggtatgtta aatgctcaag aaacaatgac
     6121 agggcatgtg aatatgacta attacgaaac actaaactca caaattggag taaaagaggt
     6181 ataaaaatgg tacttacaga acagaaacgc aaatctttgg aaaaaatttc agataaaaac
     6241 ggtgttatct cagctttggc atttgaccaa cgtggtgctt tgaaacgttt gatggcacag
     6301 tatcaagata cagaaccaac tgtagctcaa atggaagaac taaaggtttt ggttgctgat
     6361 gaacttacaa aatacgcttc ttcaatgttg cttgaccctg aatatggact tccagcaaca
     6421 aaagcattgg ataaagaagc aggtcttctt cttgcctatg aaaaaacagg ttatgataca
     6481 tcaagtacaa aacgtttgcc tgactgcttg gatgtttggt ctgcaaaacg tatcaaagag
     6541 caaggcgctg atgccgttaa gttcttgctt tactatgatg tagacagctc agatgaactt
     6601 aaccaacaaa aacaagctta tattgaacgt gttggttctg aatgtgtcgc tgaagatatt
     6661 cctttcttct tggaaatcct tgcttatgat gaagaaatct cagatgcagg ttctgttgaa
     6721 tatgcaaaag tgaaacctcg caaagtcatt gaagcgatga aagttttctc tgatccacgc
     6781 tttaacattg atgttcttaa agtagaagta ccagttaacg ttaaatatgt cgaaggtttt
     6841 gctgatggtg aagtagttta cagtaaagct gaagctgctg acttctttaa agcacaagaa
     6901 gaagcgacta accttccata catctaccta agtgcaggtg tatctgctaa attgttccaa
     6961 gaaacattgc aatttgctca cgactcaggt gccaagttta atggtgtgct ttgtggacgt
     7021 gctacttggg caggatctgt agaaccttac atcaaagaag gtgaaaaagc agcacgtgaa
     7081 tggttgcgta ctactggatt cgaaaatatt gatgaactta acaaagttct tgttaaaaca
     7141 gctagtccat ggactgataa agtatagtaa aaacataaaa cggaggatat tgttatgaac
     7201 agagaagaga tgactctctt agggtttgaa attgttgctt atgctggaga tgctcgctct
     7261 aaacttttag aagcgcttaa agcggctgaa aatggtgatt tcgctaaggc agatagtctt
     7321 gtagtagaag caggaagctg tattgcagag gctcacaatt ctcagacagc tatgttggct
     7381 cgagaagctt ctggggagga acttccatac agcgttacca tgatgcatgg tcaggaccac
     7441 ttaatgacta ctgtcttatt aaaagatgtg attcatcacc tcatcgaact ttataaaaga
     7501 ggagcaaaat aatgcataaa ctcattgaac ttattgagaa agggaaaccg ttctttgaga
     7561 aaatctctcg aaacatctat cttcgtgcta ttcgtgatgg atttatcgct ggtatgccag
     7621 tcatcctttt ctcatctatc tttatcctta ttgcctatgt cccaaatgct tggggattcc
     7681 actggtcaaa agacattgag accttcttga tgactcctta tagctattcg atgggtatcc
     7741 tagcattctt tgttggtggt actaccgcta aagctttgac ggactcaatg aaccgtgacc
     7801 tacctgcgac caatcagatt aacttcttat ctacgatgct agcgtctatg gtagggttcc
     7861 tcttgatggc agctgagcct gcaaaagaag gaggcttctt gacagccttc acgggaacaa
     7921 aaggtctttt gacagcattt attgcagcct ttgtgacagt taatgtttac aaggtctgtg
     7981 tgaaaaataa tgttactatt cgtatgcctg aagaggttcc accaaatatc tctcaagtat
     8041 ttaaagactt gattccattc actgtagcgg ttgttctcct ttatggattt gaactcattg
     8101 ttaagggaac tcttggtgta accgttgcag aatcaatcgg tacccttctt gctcctctct
     8161 tctcagctgc agatggttac cttggaatta cacttatctt tggtgcttat gccttcttct
     8221 ggtttgttgg tatccacggt ccttcaattg tcgaaccagc aatcgctgct atcacttatg
     8281 ccaatatcga tgccaacttg catcttatcc aagctggaca acatgcagac aaagttatca
     8341 cttctggtac tcaaatgttt attgttacca tgggtggaac aggagctaca ttgattgttc
     8401 cattcttgtt catgtggatt tgtaaatcag aacgtaaccg tgccatcgga cgtgcctcag
     8461 ttgttccaac attctttggg gttaatgagc caatcttatt tggtgcacca atcgtattga
     8521 atccgatttt ctttgtaccg ttcattttcg ctccgattgt caacgtttgg atctttaaat
     8581 tctttgttga cacattgaac atgaactcat tctctgccaa ccttccatgg gttactcctg
     8641 gtccgttggg aattgtactc ggtactaact tccaagttct atcatttatc ttagccggac
     8701 tcttggtagt tgttgatact atcatttatt acccatttgt taaggtatac gatgaacaaa
     8761 ttcttgaaga agaacgttct ggtaaaacaa acgatgctct taaagaaaaa gtagctgcaa
     8821 acttcaatac tgctaaagca gatgccgttc ttggaaaagc aggcgttgct aaagaagatg
     8881 tagcagcaaa caataatatc acaaaagaaa ctaatgtcct tgttctttgt gcaggtggag
     8941 gtacaagtgg cttgcttgcc aatgccttga acaaagcagc agcagaatac aatgttccag
     9001 tcaaagcggc agcgggtagt tacggtgctc accgtgaaat gttgccagag tttgacttgg
     9061 ttatcttggc tccacaggtc gcttcaaact tcgatgatat gaaggctgaa acggataaat
     9121 tgggcattaa acttgcaaaa actgaaggag ctcaatatat caaattgaca cttgatggac
     9181 aaggtgctct tgcatttgtt caacaacagt ttgattaata aatttgtaaa agttatttga
     9241 gacgagatta ttctttcaat tccttataat attgttaacc atatcattgc atttcatttc
     9301 cttggtttcc ggaactcaga atatcgtctc aaatgctttt tttgaaagga tttatagtta
     9361 tgactaaaac acttcctaaa gattttattt ttggtggtgc tacagctgct tatcaagcag
     9421 aaggtgcgac tcacacggat ggtaaaggcc cagttgcatg ggataagtat cttgaagaca
     9481 attattggta cacagcagag cctgcaagtg atttttacca taaatatcct gttgatttag
     9541 aattagctga aaaatatggc gttaatggta ttcgtatttc aattgcctgg tctcgtattt
     9601 tcccaactgg ctatggcgaa gtaaatgaaa aaggtgttga attctaccat aatctttttg
     9661 ccgagtgcca caaacgtcat gttgagccat tcgtgacttt gcaccacttt gacacaccag
     9721 aagttcttca ctcagatgga gatttcttaa accgcgaaaa tatagaacac tttgtagatt
     9781 atgccgcttt ctgttttgaa gagttcccag aagtaaacta ctggacaact ttcaatgaaa
     9841 ttggcccaat tggagatggt caatacttgg ttggtaaatt ccctccaggc attcaatatg
     9901 atcttgccaa agtcttccaa tcacaccaca atatgatggt ttctcatgca cgtgctgtga
     9961 aattgtataa agataaaggt tataaaggcg aaattggtgt agtacatgcg ttgccaacca
    10021 aataccctta tgatccagaa aatccagcag atgttcgtgc cgctgagtta gaagatatca
    10081 tccataacaa atttatcttg gatgcaacct accttggaca ctattcagat aaaacgatgg
    10141 aaggggtcaa ccacatccta gctgaaaatg gtggagaact tgatcttcgt gatgaagact
    10201 tccaagcact tgaagcagct aaagatttga acgatttcct tggtatcaac tactacatga
    10261 gtgattggat gcaagccttt gatggtgaga ctgaaatcat tcacaatggt aagggtgaaa
    10321 aaggaagttc taagtaccag attaaaggtg ttggtcgccg aatcgcacca gattatgtgc
    10381 ctagaacgga ttgggactgg attatttatc ctgaaggctt gtatgaccaa atcatgcgag
    10441 tgaaaaatga ttatccaaac tacaagaaga tttacatcac tgaaaacggt cttggataca
    10501 aagatgagtt tgtagataat accgtttatg atgatggtcg tattgattac gtgaagaaac
    10561 acttggaagt tttatcagac gcaattgcgg atggtgcaaa tgttaaaggt tacttcatct
    10621 ggtcacttat ggatgtcttc tcatggtcaa acggatacga aaaacgttat ggactattct
    10681 atgtagactt tgacacgcaa gaacgctatc ctaagaaatc agctcattgg tataagaaag
    10741 tagcagaaac tcaagtgata gagtaatatt acttgaaaat gtaaaaaatt gttatccgat
    10801 actttagaaa aagtacaaca cttatttaga ccaggctttg aaaaggtata tattgttagt
    10861 ttcgaagatt gtcctatgat tcctgagctt gaagctaccc cgttgctaaa atgtggtaaa
    10921 tggtatgttt caactggaaa agagtggatt tgccattctg atttagagtt atcagcattt
    10981 gaatgggaat ttttacaatc actcgatgta gaaatacgtg agactattca cttcgaggta
    11041 aattatttgc ctttccaata ataaaaagaa aggaattagt tgtgactatc gaattaaaaa
    11101 acgagtatct tactgttcag tttaaaacac ttggtggtca actgacttcg attaaagaca
    11161 aggatgggat agagtatctc tggcaagctg atccaaatta ttggaatgga caggcgccta
    11221 ttctatttcc tatctgtggt agtttacgaa atgattgggc tatttataga cctcaagaaa
    11281 gacccttttt tacaggtctt attcgtagac atggttttgt tcgaaaagaa gaatttattc
    11341 tggaagaagt caatgataat agcgtgacct ttagtattaa accaaatgct gagatgcttg
    11401 ataattacct atatcagttt gaactgagag ttgtttatac cttgaatggt aaatcaatta
    11461 gaaccgaatt tcaggtaacg aacttggaaa tggaaaaaac aatgccttac tttattggag
    11521 cccatccagc attcaatagt cctttagtag aaggtgaaaa ctatgaggat tattatattg
    11581 aatttagtga ggttgagtct tgctcgatac caaagagttt tcctgagaca ggattattag
    11641 atttgcaaga tcggacacct tttttggaaa atcaaaaaat tctcgatttg gactactcac
    11701 ttttttccca tgatgccatt acacttgacc agctcaagtc tcgtagcgta acgcttcggt
    11761 caaggaagtc tggtaaaggt cttcgagtag attttgatga ttttccgaac ttgattctgt
    11821 ggtctacaac taataaaagt ccattcattg ctttggaacc ttggagtggt ctctccactt
    11881 cacttgaaga aggtaatttc ttagaagata aaagacaggt aacgaaaatt tcacctcaag
    11941 aaacttctag aaaaatctat gatattacta tccttaatta aaatttgtta ttatttgagt
    12001 cttcaataag atttgaatac tcttaccttg tgatgataga ttggtcttct ccgtgccagt
    12061 cttacatcgc aaggttttta agttttttgt gggtctatgt caactgtagt gggtgacgaa
    12121 aagctaatat ctaaagacag tctggtagta aaaaataaga ttatttacta cttcttgatt
    12181 taaaataata gtggagccat taatttgtgg caaatttaat ttaatatttt gcctcaaatt
    12241 agccacactt tttatagaac taagaggaaa aatgaaattt tcttcctatt aatatagttg
    12301 tttgaaatct taaatatagc g
//
LOCUS       DAFFSV010000091        12278 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFFSV010000091 DAFFSV010000000
VERSION     DAFFSV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.091"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(398..985)
                     /locus_tag="LR110_002098"
     CDS             complement(398..985)
                     /locus_tag="LR110_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823050.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1160..2482
                     /locus_tag="LR110_002099"
     CDS             1160..2482
                     /locus_tag="LR110_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL4823051.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2637..3413
                     /locus_tag="LR110_002100"
     CDS             2637..3413
                     /locus_tag="LR110_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL4823052.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3406..3975
                     /gene="rnmV"
                     /locus_tag="LR110_002101"
     CDS             3406..3975
                     /gene="rnmV"
                     /locus_tag="LR110_002101"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBL4823053.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3968..4852
                     /gene="rsmA"
                     /locus_tag="LR110_002102"
     CDS             3968..4852
                     /gene="rsmA"
                     /locus_tag="LR110_002102"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBL4823054.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5303..5932
                     /locus_tag="LR110_002103"
     CDS             5303..5932
                     /locus_tag="LR110_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823055.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6056..7165)
                     /gene="ugpC"
                     /locus_tag="LR110_002104"
     CDS             complement(6056..7165)
                     /gene="ugpC"
                     /locus_tag="LR110_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBL4823056.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7374..7796
                     /gene="mgsA"
                     /locus_tag="LR110_002105"
     CDS             7374..7796
                     /gene="mgsA"
                     /locus_tag="LR110_002105"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBL4823057.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            8119..8655
                     /locus_tag="LR110_002106"
     CDS             8119..8655
                     /locus_tag="LR110_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL4823058.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8695..10458)
                     /locus_tag="LR110_002107"
     CDS             complement(8695..10458)
                     /locus_tag="LR110_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4823059.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10461..12176)
                     /locus_tag="LR110_002108"
     CDS             complement(10461..12176)
                     /locus_tag="LR110_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4823060.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3843 a         2258 c         2491 g         3686 t
ORIGIN      
        1 tattacttga agccaatcat ataatacaga aacagagaac tatccaaaat aaatcctaga
       61 aaaagaagta cgagcttcgc tgatttttgt taatttagaa taatactaat aggaatcgta
      121 aaaacagaaa aaaagcataa tgataaaggg tcgggcagat tttaattgat tcatctggta
      181 ctctttttta ttgtccttac attcataaag tttgaatgca atgactttat tacatctata
      241 tttatatcta acagtagcgt tgatgtgtta attataagtt ctatcacttt atgttcaatt
      301 agtttgtgct cttatatgtg tgattgaaaa tatagataaa aaaacaatca tcggctttga
      361 cttcgatgat tgttttcttt attttgtaaa aatcctatca cttgatttta tggtaacctt
      421 ttatttttgt cttcaaacgg cttggtaaga gagcagtaag cagtgcaaat gggatcgaaa
      481 agacatacca gaaaatcgta cgtccgatac cataatgttg atagagcttt gtcaacatca
      541 cgtgtaacaa gatcaaccct aaaagcaagg agccagctat aaaccaatga ttatcccaat
      601 gcatgacttc actaagtgcc atcactccaa aaggatagaa ataaaaatag atcccgacta
      661 aggtcatccg ccagttcttt gtaaatgttc cgatattcaa taaaaagaaa atcgctgaaa
      721 gcaaaatacc gataggcgga aaaagcccaa ccaaaaagga atacaacatc ccccacaaga
      781 tcatcacagg acctttgatc aatgcaacga ttgcgatgaa caaagtcagc aatattagtt
      841 tttgtgataa cgttgcaaca gtcattaatc caaataaaat taatgtgaac caatgccaat
      901 gggactgctg ttgttcttta cgggaaaaat cgacccggtt ggataaataa tctgctaatt
      961 tcggataaga acgcttaaaa tccataaact gttcctcctt cgtggaaaag gtaggtttag
     1021 tatacgaaaa ataaaaaaga aatacatccg tcttaagagg gagtttttca ctgaaagtgg
     1081 agaaaaaaga atggtaaatt aaaatattct cagttatact gaaaattata ccaaatttca
     1141 aagaagaaag aggatgggaa tgaaaggcaa actaaaacga gaaatcaatc tgtttggcgc
     1201 attagctacc gttatgggaa ccgtgatcgg tgcaggcgtc ttcttcaaaa ctgctgctgt
     1261 tactgctagt actcagtcta caagtttgac attgctagct tggcttttgg gtggtttctt
     1321 aacgatctgt gctggattga cagtggctga attagctaca gcaatcccgg aaactggtgg
     1381 agctgtaaaa tatattgaag ctgcgtatgg aaaattgccc agctttttat taggatgggc
     1441 gcaaagcttg atttactttc ctgctaatat tgctgcatta tcgattatct ttgcgacaca
     1501 attgacgaac ttattacaat tatctaccga ttatcttttg ttgattgcag tgatcaccgc
     1561 tgtttctgtg acagggttga atttgttagg gacaaaagtc ggtgcttctg tacaatcagt
     1621 gacgttgatt gttaaattaa taccaattgc tgtgattgtc atatggggat tattgacccc
     1681 aggacaagga actgtccagt tatttccaat cgaagctgga aaagatgtta cttttgtcga
     1741 aggattgagc agtgcgttgt tagcaacgtt gtttgcttat gacggctggt taggtgtcgg
     1801 tgcaatggca ggggaaatga aacggccgga aaaagatttg cctaaagcaa ttattcttgg
     1861 tttgagtttt gtaacagtcg tttatctatt gatcaatttt gtttttttga aaactttgcc
     1921 gattgatcat cttgccggta atttgaatgc agcttctgaa gcatcagacg tcatttttgg
     1981 aggaatcggt ggaaagctgg tcactattgg gatcttgatc tcggtatacg gtgcattgaa
     2041 cggttataca ctaactggga tccgcgtgcc ttatgcgatg gctttagaag acgagctacc
     2101 tttcagtaaa cagctgacaa agctttcaaa gaagtttacg gttccttatg ttcctgcggt
     2161 tttccagtta gcagtagctt gtattatgat gagccttggt tctttcgact ttttaacaga
     2221 catgttgatt tttgtgatgt ggctattcag tttactgatt tttatcggtg tctttatttt
     2281 aagaaaaaaa gcgccggaat tgccacgtcc atataaagta ccactttatc caatcgttcc
     2341 gattattgcg attctcggtg ctattttcat tttaggtatg acgatgatga cgcaaacgaa
     2401 attagcattg atcggtatag gtgttacctt gataggtatc ccagtctatt atcaaaaaaa
     2461 gaaaaaacgc tcagaagagt aaaatatttc aacaacagac agaaaaagta cgaactaggt
     2521 cctctagatc ttcgtgcttt ttctatttca ataaaacaag ttcttctatt ttgatttttg
     2581 ctacttctgc aagtagtgct tatgtgctaa agtaagaatg aaaaggagag ataaacatga
     2641 tatttgattc tcatacccat ttaaatgctg aacagttcaa tgaagatatt ccagaaacga
     2701 ttcagcgagc acaagaaatg ggggtaacga aaatggcggt cgtgggcttc gatacgccaa
     2761 cgattgaaaa gtctttgcaa ttaagccatg actatccaaa tatatacagt attatcggct
     2821 ggcatccgac agaggctggc agttatacga aagaaatcga aaataaatta caagaacaat
     2881 tgactttacc aaaggttgta gcattagggg aaattggctt ggattatcat tggatggaag
     2941 acccgaaaga agtccaagat aaagtatttc gcagacagat tgctatcgca aaagaaatga
     3001 acctaccaat cagtattcat actagagaag cacttgaaga cacgtatcaa atcctaaaag
     3061 aagaagatgt tcgagatatc ggtggaatca tgcatagttt cagtggtgat tatgaatggg
     3121 caaaacgatt tcttgactta gggatgcata tttcatttag cggtgtcgtc actttcaaaa
     3181 aagcattaga cgtccaagag gctgcagcaa atgtaccaat cgaacgatta cttgtggaaa
     3241 cagatgcccc ttatctggca ccagttcctt ttagaggaaa gcgaaatgaa ccaggctata
     3301 ctcgttatac agtagaaaag attgctgaac ttcggaatct tccgacagaa gaagtggctg
     3361 cccaaacatg gacaaatgcg caccgattat ttggtctaag cgaacatgga taagatgaag
     3421 atccaagaga ttatagtcgt tgaaggaaaa gacgatacaa gaaggctgcg ggaagtagtt
     3481 gatgcagata cgatagaaac aatagggtct gcaatcaatg aagagatcct gatgcaaatt
     3541 gaacatgccc aagagactcg tggagtgatc gtttttaccg atcctgattt ttcaggagaa
     3601 aaaataagaa aaatcattat ggaagctgtt cctcaggcaa aacatgcttt tttgccgcgc
     3661 acacaagcta aaccaaagaa aaagggaagc cttggtgtcg agcatgcaag tgatgctgct
     3721 attttagaag cattgaaaaa ggtcgtgacg ccagacattt cccaaacggc tgtttctgaa
     3781 atcactagac aagacctttt agactatggt ttaatagctg gggcacaagc aaaagaaaaa
     3841 cgagaaaaac taggtgacga gttgcgcatc ggttacacga acggcaaaca attggaaaaa
     3901 cgtttgaaga tgtttcgtat tacgccgaaa gaactcgccc aagcaatgaa aatagtggag
     3961 gaacaacatg agtgaatatc gtgaaattgc gacaccctct agaacaaaag agattttatt
     4021 gaaacatggt ttttcattta aaaaaagttt aggacaaaat tttctgactg aaccaaatat
     4081 tttaaggaaa atcgtagaaa ctgcgcaaat agatgaacat acgaacgtga tcgaagttgg
     4141 accaggaatc ggtgcattga cggaacaatt agccaagcat gcaaaacaag tcgtagcatt
     4201 tgagatcgat gatcgtttga tccctgtatt ggctgatacg atgcaaccct atgacaatgt
     4261 gactattgta catcaagata ttttaaagac cgatttgtca acagctgtta gagaaacatt
     4321 tcatgaggag cttccgttaa aagtcgtagc gaatttacct tactatatta cgacaccgat
     4381 catgatgcat tttcttgaat cagatttggt tgtggatgaa ctagtcgtca tgatgcaaaa
     4441 agaagtagct gatcgtatct ctgctgaacc aggaacgaaa gcttatggtt cattatcgat
     4501 tgctgttcaa tattatatgg aagccagttt agcattcatc gtaccaaaaa cagtatttgt
     4561 ccctcagcca aatgtagatt cagcgatttt gaaactgaca agaagagata taccggcagt
     4621 cgaagtgaca gacgaaaaag catttttccg attgacaaaa gcggcatttc agcaaagaag
     4681 aaaaacgctt tggaataact tgcagcattc gtatggcaaa gatgaccaga caaaagcatg
     4741 gttagcaaaa agcttggaaa cagctggaat cgatccaaaa cgtcgtgggg agactttgtc
     4801 attacaagaa ttcgccgcat taagcaatgc gatgtcagaa aatatgcagt aaaaaaagag
     4861 gttgtgacag aagttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa
     4921 aatagttcct catgtttttc gtttgtttgg gcttattacc gcagatgcaa gcttttgaac
     4981 accgtttaag aaagaggttg tgggcctgcc gtttaactct aagtcacaag gaacaatttc
     5041 aaaaaacagc ttctcatgtt tttgagttag ttggtcttat tacttgatgc aagcttttga
     5101 ccactattat gccgaacaag gagatgtgac acaaattttg tcacatctcc tatttttgtt
     5161 tcttgatcaa aaaattctta tacttgatac tcacactcat tttgtatgga tttagaaaag
     5221 aatatgtgtc ttgacagtta ttagtaagtg agttatacta agtcgacatt tatgtgtcaa
     5281 gacatattag gagagagaaa aaatgagaaa aaattcagtt cgaaaactaa cgatttctgc
     5341 tttattgatt gggatggggg tcattatccc aatggtcatg cctaaaatca tgattccacc
     5401 agcttccttt actttggcgt ctcatgtgcc attatttatt gcgatgtttt ttacaccagg
     5461 agttgcagtg gctgttgcgc taggtacgac atttggtttc tttttaacaa cgccggttat
     5521 catcgcattg cgagcattgt cacatcttgt gtttgcgttg attggggctt ggtacttgca
     5581 aaagcatccg agtatcgttt taaaaaatgg acagtttact tttgctaatt ggcgtttcca
     5641 agggttcaat tttgtgatcg gtgtgattca ttctttagca gaaatgctag tagtcagcat
     5701 cttttacttt attggtaata tgcctgaaac atattattct caaggatatt tttatacagt
     5761 attcatcttg atggggattg gtgggcttat ccacagttta gttgattata atattgctta
     5821 ttttatagca ggaacactca gcaaatattt tgatatcccc gtatttacgg cagcaaaaaa
     5881 agaaaatcgg aaagagtcga ttcaagggta cgcgaagaaa acgacatatt agacatgatc
     5941 tctcaaaaaa aaaactctca tctagtaatg gataataaat cctctaagat gagagttttt
     6001 tgtcctaaaa agagctgcac caacatcttc tggcacagct ctaacagggg gcaatttatg
     6061 gaagtttgat gacttgttct gtttctttat caaagaagtg gcctttgctg atgttgaagg
     6121 caaggtcgac tatttcgcct ggtttatgga aatctcttgc atctactttt gagatgaact
     6181 cagtagaacc agtttttgtg taaagcatcg tttcagctcc aagtaattcg gaaacgacaa
     6241 cttctgattt cactacggct tcaggtgaag cttcaagggc tacttgttca ctgtggatgt
     6301 cttctggacg aataccgaag ataacttctt ttccttcata acctttttca actaatactt
     6361 tgttttttcc ttcaggaata cgaagcttaa gcccatgtcc atctgtgata acaccatttt
     6421 tcaatgtcac attaaagaag ttcatggcag gagatccaat aaagccggca acgaacatat
     6481 tgacgggtgt atcataaact tctttcggtg taccgatttg ttggataaat ccgtctttca
     6541 tgataacgat tcggtcagcc atagtcattg cttctgtctg atcgtgagta acatagatgg
     6601 tcgttgtttc aagacgctgg tgaagtttgg caatctcagc acgcatggct acacgtaatt
     6661 tagcatctaa atttgataat ggctcatcca ttaagaagac ttttgcatcc cgaacgattg
     6721 cccgtcccaa tgccacacgt tggcgttgtc caccagataa agcagctggt tttcgtttca
     6781 agtattctgt cagacctaag atctctgctg cattgtccac acgttttttg atgtctgctt
     6841 tatcatattt acgtaatttc aagccaaaag ccatattatc aaaaacagtc atatgaggat
     6901 acaatgcgta gttttggaaa accatggcaa tatcacggtc tttaggtgcc acgtcattca
     6961 tgacttttcc gccgatattt agttctcctt cagagatatc ttccagtccc gcgatcattc
     7021 gcaaagtagt tgactttcca caaccggaag gaccaacaaa aacgataaat tcacggtctt
     7081 tgattgatag attaaaatca gttacagaat aattttctgc attgtcatat tttttatata
     7141 cgtttttcag agccatttct accataatac gttccacact ttctttttta gttttattaa
     7201 ctatttaaag tataaatgaa agcgttaact aacttcaatg tcatgttgaa caagaatttt
     7261 ttcttttttt tgtcaatgtg ctagttttaa gctttaataa atatccataa gaaataaaaa
     7321 ctgatacact ggagtaaata agtatgttaa caatatcaaa aggagtgtct cttatgaaaa
     7381 tcgctttgat cgctcatgac cgaaaaaaac ctatgatggt gaaattagca acagcgtata
     7441 aaacaatatt ggaaaaacat gaactttttg ctaccgggac gactgggcag aggatcacgg
     7501 aagcaaccgg cttgccgatc catcggttta aatccggacc gttaggcggg gatcagcaaa
     7561 ttggagccat gatttcggag gatcagttag atttggtgat ttttcttcga gacccgctgg
     7621 ctgcacagcc ccatgaacca gatgtaacag cacttatccg tttatctgat gtctatgaga
     7681 ttccgttagc tacgaatatc ggaacagctg aggtgctact aagaggactg gaagccggct
     7741 tcctagattt tcgaagaatc gtccatgaaa tagacaagca tccactttca ttttgatgga
     7801 taagcgtatg gaaaaataac gtttaatgtt cgcctatatg tagctataaa agctaaacaa
     7861 actgataata tgcgatataa tacgaacgta ttggataata taaacaataa tattagtttt
     7921 tcgtatttgc agtaaatgtg aaaatatgct atagtttctg tgggaaaaga tcccaaaaat
     7981 aatacagagt agaaaataaa gcgttaagtg ctaagaggat gggatgttgc ctcttggacg
     8041 aaggacgtaa tgttcgcggt tttattttcg cattcgctgc atgatacgat gtagcagctc
     8101 ttaaaggaga tgctaaaaat gaagaaacac cggttagacg cacgtatgat tgctattatg
     8161 ggcttattga ttgcattgat ggtcactttg tcccgactgg tagcaatcga gaccccattt
     8221 attaaaatca gtgtcacatt tattccacaa gtgatcatgg gcattctttt tggtcctttt
     8281 tggtcaggga taggcgcagt tttagctgat ttagtaggaa tggccctctt ctcgaaatct
     8341 gcattcttta ttggatttac attgaatgca tttattgaag gagcaatcta cggattcttt
     8401 ttctatcgaa aagagatcac ttggaaaaat gccattctgg ctacgcttag tgtcacgctg
     8461 atcatcaact tatttttgac accattatgg cttgcgttga tgtaccatgt gccattgttc
     8521 agctgggtcg tttgggcgcc ccgcctgtta aaaacagtaa tatggttacc gattcaatca
     8581 attgccattt attacgtagg acgtagtatt ccttacaaaa aaattttaag aagtttagcg
     8641 atccacgcta aataaaaaac aagcagtctt cccatcccag aagactgctt gtttttattg
     8701 tgctgtgaat tgtgcttgat agagccgtcg gtaataacca tctttttgaa gcagctcatc
     8761 atgggtgccc atttcgacaa tagctccctg atccatcact aatatctggt ccgcattttt
     8821 gattgtggac aggcgatggg caatcacaaa actggttttt ccttccatca ttgctaagaa
     8881 ggcttgttgg atttttttct ctgtcaatgt atcgactgag ctggtagctt catctaagat
     8941 caacataggg ggattactga tcatcgttcg agcaatcgtc aacagttggc gttgtccatc
     9001 tgatatcttt aacccttgac cgccaatctt agtatctaat ttttccggca aacgttctac
     9061 aaattcatac atataagagg cttttagggc atcattgatt tctgcgtcac ttgcttccgg
     9121 attcccaaaa cgaagatttt ctcgcaatgt actgtcaaat agccaagtat cttgtaacac
     9181 cattccaaaa cttttacgta atgtatcacg ttggatagag cggatgtcat ggtcatcgac
     9241 tgtaattgtt ccttttgttg tgtcataaaa acgcatcagc aaattaacaa gtgtggattt
     9301 tccggcccct gtttttccta ctatagcaat ggtttgacct ggataagcag taaaattgaa
     9361 atcttcgatc aaacgctgct tggggtcata agaaaaagaa acatgatcaa aagagacttt
     9421 tcctttgata tttttgggtt ccaaggcata ggcatctggc aaatcaggct tttcttctgg
     9481 ctgatcgatc aaatcaaatg ctcgttctag accagaaaaa gcggtttgca gttgtgtcgt
     9541 gattccagaa agttcgataa atggtttaga aaactgactt gagtagatcg taaagctgga
     9601 aatcacacca acagtgattg acgaaccact cttcaatgcc aataggccgc caactaaacc
     9661 aatcgataaa taagcaaggt gatctacaaa acgagaagac gggtttgtca aggaagaaga
     9721 aaattgtgcc cgttgtcctt tttcatatag atcagcattt agcagttcaa attgctgttg
     9781 actagcttct tcttgctgaa aagctttcac gattttctgg ttaccgattc gttcggtcac
     9841 aaagcccgag atatccccaa taatcctttg ctgtctatcg aaatttgttt gtgaagcacg
     9901 agcaacgatc caactcacaa ggaaaataat tggtgtagaa caaagaacga ccaatgtcag
     9961 aagtggactt agacgcatca taaaaataaa cgaaagaatg atgattgcca ctccagaaaa
    10021 taattgattg aaggccgcag aaattgcaat ggaaatattg tccatatcat ttgtaaatcg
    10081 actgacgata tttccgtggg agttttgatc ataataacga agtggcaatc gattcagatg
    10141 agcaaatgca tcttttctta attttgcaac ggataagtaa gctaaccgat tgcttaacac
    10201 ttggatgagc cattgactga ctacgttgat taaaacgata ccaccgaata gaataagtaa
    10261 atggaacagt cgattgaagc ggacctgtcc ttctccaatc atttgatcga tactttgacc
    10321 aatttgtaac gtcatcacaa ctgttgcaat cccattgatg attcctagta tcacagctag
    10381 ccaaatgtct tttgcatagg cagtaaggta aggaatgaat cggcggagcg tctgcaagga
    10441 taattttttt actttcataa ctattcttcc tcctgtgatg cgacgatttc ctgatattct
    10501 gctgattgag caagtaattc ttcatgggta ccctgagcag caatcttacc ttgatccatc
    10561 actaagatat gatgtgcttc ttgaatagag cggacacgtt gggagataat aatcactgta
    10621 ctgccaagat aagtctgtag cgccttacgc aaatccaagt ctgtttgata atccaacgca
    10681 cttaaagagt catctaaaat cagtaatgct ggcttggcaa tcaaagctct tgcaatcgtc
    10741 agtcgctgtt tttgtccgcc agagaaattt ttcccatttt ccatgacttt tgtatctaaa
    10801 ccatttggaa gctgacggac aaattcttct gcctgagcga ttttcagagc ttcccaacat
    10861 tctttttctg tggcgtcttt cttaccccat tgaaggtttt cacgaatagt cccggtaaat
    10921 agtacagaag tttggggaac aagagcaatt tgctgacgga gatgatccag ctgccaagaa
    10981 cgaacatcca ttccgtccat tataactgta ccggaactga catcatagaa acgcggaatc
    11041 aattgtgtca aagtcgtttt cccgcttccg gttggaccaa tgattccaag gactgtattt
    11101 cgcttcagct gaaatgtaat attttggaga gaaagaccag aatcaggagt ataacggaag
    11161 tcaacatggt caaaagcgat aattgcttca gaagaaaaat cgggagtagt actgacctca
    11221 gcttcaagaa tcgaattttc tgtgtctagc acctctttca cccgatttcc tgaagcttcg
    11281 gcacgtgtga agatcacgac gaggtttgaa acaacgatca gtgccaaaag catttgattc
    11341 atataattga tcaaagcaag aacttgtcct tgttgcaagc taccaatatt tactttgatc
    11401 ccgccaactg tcagcaaaca gatgatcccg acattcatga tcaaggttgt agcaggtgat
    11461 aatagggcag agatattgga aacacgtaaa taattgttcg ctagatcatt tgtgctttta
    11521 tcgaacttct ctgtttcttt ttttgtctgc gcaaatgcgc ggatgacacg aacaccgcta
    11581 agattctgac taacgtgttc attgagctga tctagttttt cttgcacttt ttggtagaga
    11641 ggaacggttt ttttgatgat aaaaaataaa atgattgaga aaataggcaa taatgctaag
    11701 aaaaacagac ctacttccca gtcgatcaca aaagccatga ccactgaccc gatacttaaa
    11761 aacggtgctc tgatcaataa acgaatgacc attgctaaag ctagctgaag ctgattgatg
    11821 tcgtttgtca ttcgggtgat caaggtatct gttccaaaat gattcaactc gttgtgagag
    11881 aaagtattga tttttttgat tagctgatta cgtaactctg taccaaatcc ttgtgaagct
    11941 actgaagcat agtattggca aatgatagca caaatcagac caatcacaga catagcgacc
    12001 atccataagc ccatttcaat gatttttcct cggtcattca tttttagtcc attatcgatc
    12061 aaggaagcca tcattaaagg caggacaagt tcgaagacag cttcaagaaa tttgaagaac
    12121 gggcccaaaa tgatttgctt acgatagtct tttgcataac gtaataactg aatcatatga
    12181 ttcctcctat tatttatttg tccacaattg aaaaaacaag tactaccaca ttgtagctta
    12241 ggaaatttag aatgaaaaga gaacaagcct tttcataa
//
LOCUS       DAFFSV010000092        12211 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFFSV010000092 DAFFSV010000000
VERSION     DAFFSV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.092"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..340)
                     /locus_tag="LR110_002109"
     CDS             complement(<1..340)
                     /locus_tag="LR110_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4823061.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
     gene            992..1963
                     /locus_tag="LR110_002110"
     CDS             992..1963
                     /locus_tag="LR110_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBL4823062.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(2041..2277)
                     /locus_tag="LR110_002111"
     CDS             complement(2041..2277)
                     /locus_tag="LR110_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL4823063.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(2246..2377)
                     /locus_tag="LR110_002112"
     CDS             complement(2246..2377)
                     /locus_tag="LR110_002112"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL4823064.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2453..2836)
                     /locus_tag="LR110_002113"
     CDS             complement(2453..2836)
                     /locus_tag="LR110_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL4823065.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2869..3654)
                     /locus_tag="LR110_002114"
     CDS             complement(2869..3654)
                     /locus_tag="LR110_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBL4823066.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3832..4932
                     /locus_tag="LR110_002115"
     CDS             3832..4932
                     /locus_tag="LR110_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL4823067.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4922..5746
                     /locus_tag="LR110_002116"
     CDS             4922..5746
                     /locus_tag="LR110_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBL4823068.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5743..6558
                     /locus_tag="LR110_002117"
     CDS             5743..6558
                     /locus_tag="LR110_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL4823069.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6555..7385
                     /locus_tag="LR110_002118"
     CDS             6555..7385
                     /locus_tag="LR110_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4823070.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            7392..8423
                     /locus_tag="LR110_002119"
     CDS             7392..8423
                     /locus_tag="LR110_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4823071.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8530..9803)
                     /locus_tag="LR110_002120"
                     /pseudo
     CDS             complement(8530..9803)
                     /locus_tag="LR110_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            10030..10215
                     /locus_tag="LR110_002121"
     CDS             10030..10215
                     /locus_tag="LR110_002121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823072.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            10339..11055
                     /locus_tag="LR110_002122"
     CDS             10339..11055
                     /locus_tag="LR110_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBL4823073.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10957..11477)
                     /locus_tag="LR110_002123"
                     /pseudo
     CDS             complement(10957..11477)
                     /locus_tag="LR110_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11765..>12211
                     /locus_tag="LR110_002124"
     CDS             11765..>12211
                     /locus_tag="LR110_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4823074.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNA"
BASE COUNT         3508 a         2272 c         2777 g         3654 t
ORIGIN      
        1 ggcgaacata attctttcca aaagttgaga aatgggggat tttctcctcg aaggagtagc
       61 ctaaaaacca gcggtaagcg acattggtat cgacttcctt aatggtttgt cgcatggaac
      121 ggataccgaa taaatactga ataaacacca atttgatcag aatcactgga tcgatacttg
      181 ggcgacctaa atcggagtaa gtggcttcga cgattggata aataaattca aaatcaagcg
      241 cggcatcaat ctttcgaacg agatgatctt gagaaactag atcatcaatc gtaagcatcg
      301 caaattgatt tctaccattc gtattgtttt tagacatcat agctatcact ccttgcattc
      361 gttttcataa atctattata aaagaaaaga ctgtagacat catccataaa atggactttg
      421 tctacagtct ggataacgtg attttttgct gtaatgacaa ggttttagct gtttcagata
      481 ccatgtatcc aaaaatagtt tattgagaaa ataggagagt atttgatact aactagtagg
      541 aaaaaatgag ataaaaagta aatggaatca tttaaggtaa tctgaaataa aaaatataca
      601 gccctgtggt actttaccta caaggctgta tatatttttt actctcagcg tttttgactc
      661 cgtgtctgac tccgttttat tgattttgaa tgttattcaa tgaccattta acaggtcaaa
      721 tttgatgttt ttgattccgt atgacgccgt ttgatatcta gtgatatagt gatttgagtc
      781 tacatcgaat aagggaataa accaataaaa aatgatttag aatattaaaa taacaaaaaa
      841 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacatcc
      901 tagtatattt atatgagcta attttatgat taaatggtaa gtttttcctt gtgtgtttta
      961 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga acagtattaa gaatagaaca
     1021 gggttctcta catgatggtg ctggtttacg gaccgttgtt tatttaaagg gctgcccatt
     1081 acgctgtgcc tggtgttcga ttcccgaatc acaaagcaag cagattgaaa aaggatttgg
     1141 ccagaccatg accgctgaag aggtaatgga tgagattgag aaggatgctg ttttttactt
     1201 tcactcggat ggcggagtga ccattagcgg aggcgaggca ctggttcagg cggattttgc
     1261 taaagaaatt ttgcaaaaat cgaaatacat tggaatcaat accgtactgg aaaccagctt
     1321 ttgtggtgct tataatgaaa tccaaaaggt agctccctat gtggatacat tgtttgtgga
     1381 tgtaaaaatg tttaccagca agctccacaa acaatggacc ggcttagata accaacaaat
     1441 tttgaagaat attcgtcgct ttttaattga gtatcctaac tgcgaagtgc gaattcgagt
     1501 tcctgtggtt ccaggtatca atatgaacct gaccgagctg ctgacgattg cttgttttgt
     1561 ggcagatctt gatcggttcg taccgttaga attactaccg taccactgct atggtatgca
     1621 cggttaccaa gcattgggac tggagtatcc gttggcagac acgccagcgc cttcacctaa
     1681 agaaatgttt gcattggcag atcaactggc tcgcacggtt cctcatttgc tggtcacagc
     1741 tttaaccaaa acatttattt atttattgaa tccaagcttt atcttatgga taaatgaaag
     1801 acactcacta gctgcagcca gctcaagaat agagcttttg ctgaagtcag tgagtgtctt
     1861 tttacgatgt tttttcgaag ttaatttgtc gaatgaacac tggctcaatc ggtacatctt
     1921 cactgtttgt cggctgagct gcgatcgctg ctacgatttc taacccttgg tatacctgtc
     1981 caaaaacagt ataacgacca tccagctccg gtagtcctcc aagctccaag tatgcttgct
     2041 ttacattagc aggtcagcct gcgcctttgt gttttgcaca ataaaaaact ggttgccgtt
     2101 ggtgtggggg ccgtggtccc ctaaggccaa ggcgccatta aaatgataaa gtccgcgatg
     2161 cacttcatca gcgaaaaagc cctcatagct ggaaacagct tcatcaccag aaccatccaa
     2221 agcgccacct tgaataacaa attccttaat gacacgagcc gaaaggtgtg ttgtcataaa
     2281 cgccttgctc agctagggag cgccagttgg ctacagccag gggagcttgg tcaggaaata
     2341 agcggagacg aatttcacca gcatcggtga ccagcacaca aacttcttcc tgctgcggtt
     2401 tttctaattg aaagagagga aggtttgcaa ctgaaacgcc ttgatcctgc catcaaatat
     2461 cgaaaatccg ccactcgacc ttcgcctgat caccaaggcg agccattccc aataaaacgg
     2521 tataggcggt ttcatccgct tgaaaggtca attgcagctg tcctcgtaaa tcgttgtagt
     2581 catctggggc ggctacttga taaaaaagat gaaactgcgc atgcaaatat tccaaaggct
     2641 gatctttaaa ggctgcaaaa taagcctgaa ctttttcccg ctgcaagctg tcagccacca
     2701 aaaaattttc cagttgttgt tttggaacgg ctgctaacaa ggatcggcaa accgcctcaa
     2761 ttttcaacgc atcccaatgt cgcgcaatta aataatggat gggtgcctct tgattgtagt
     2821 cccacaggtt gctcattttt ttcaccactt tcttctatta tataggaatc acaaaatcgg
     2881 cagcggcgtc aggcggcgtt cttttccttg aatgactagc aacttttcta cctgacagtc
     2941 tgccaacagc tgactcattt ttttaaagcc cagctgataa tcttcggcaa catgagcatc
     3001 cgagcctaag gtaaagagct ttccgccgac actttgatac aaaggaacgg cataacggta
     3061 aaggtcttcg ttgccgtatt tcaagaagct cttggcattt aactccatcg ccaattctcg
     3121 ttcaacaacc tttttgaaaa tcgttagcag ctgagcctca aaatgctccg cgaattcttc
     3181 cgctgtgaag gtgaagcggc gtaatccata atcaaagtga gtcaaaatat ctccttctgg
     3241 aaaatccgtc aagacctgca acatctgatc aaaatattcc ttggtagttt gaatgggatc
     3301 tttggttaaa accacatcgt ccatataatc aaagcggcca ttttgatgaa tacttaccag
     3361 ctttaaatca taaggatgct gagcgaggta atccacgatc cgctcttctt gtcctggaac
     3421 aacgccaatc tcgatacctc gcagaaattt ggtggaatag gtttctttta aagagtccag
     3481 tttgtttata taggctggat aatccggaat gtcatctttt ccggtagatg gattgtttaa
     3541 atcaaagtga tccgtagaaa cgaaaaactc cggctgatag gctaaatagt tttcaaattt
     3601 ttcctctgaa tcaaaggaaa aatacgtgtg gagatgctga tcataatact gcatagggag
     3661 cctccttttt tctactatca taccgcaaaa gctttagaag gttaacggga attcgtcgaa
     3721 atttacaaac catatacaac ctttacattg ttcatgaaac cttttcaaga aactgattct
     3781 ctcattacaa agctttgtta gaatggaatt atcgaaaggg gagaagaatg aatgaaaatg
     3841 aatgaaaaaa ttcgtaacat gactatcggt ttattttgtg cagcaacagt aagtatcctg
     3901 ctgacaggct gcgtctcagg tgaagcaacg aagcaatcgg ctgcggcaga cgactcttta
     3961 gatacgatta tagaaaaagc caaggaagaa ggagagattg ccagcttggg aatgccggat
     4021 acttgggcaa attgggtagg cacttggaac gaccttgaaa aagaatacgg cttaagtcac
     4081 gtggatacgg atatgtccag tgcagaagaa ttggctaagt ttgaatcaga aggaaaaaat
     4141 ggaaccgccg atattggtga tgtcggtatc agcttcggtc cgttggctga aagcaaagag
     4201 ttaaccttac cttataagac caactattgg gaggaaattc cggattgggc aaaggatgat
     4261 gatggggatt ggttgttaag ctatacggga accatttcct ttatcaccga taaagagaat
     4321 gtgaagcaag caccgactag ctggaaggag ctgcaagagg gtgactatca agttagcgtg
     4381 ggggatgtga cgattgccaa ccaagcacaa tttgctgtat tggctgcggc gattgccaac
     4441 ggaggtgacg aaaaggatat tcagccaggt ctgaattact acaaaaaaat ggcagaaaaa
     4501 ggccgtttgt ccaccgttga tgccagcgta gcaaatcttg aaaaaggtga aatcgatgtg
     4561 gcagttgttt gggacttcaa tggactaaat tatcgtgacc aaattgactc cgagcgtttt
     4621 gaggtaactg tgccaacaga cggctccatc attagcgggt atacaacctt aatcaataaa
     4681 aatgcccctc atcccaatgc ggcaaagcta gcacgggaat atattttatc tgatgaaggg
     4741 caaatcaatt tggcaaaggg ctatgcgcga cctattcgca aggtagagct gcctaaagaa
     4801 gtagctgaaa aattactgcc ggaggatgct tatgaagcgg cggtgccagt tgaagacaat
     4861 gaagcttgga acaagacctc tgtagagctt ccagagcttt ggcaggcgga ggtactgacc
     4921 tatgcaaagt agacttgaca aactgattgt agttatacta gacggctgtc gctacgacac
     4981 agccgtcgcc cagcttgggt tcatgaatca tttagttgag caccagcaag caagcttatt
     5041 gaaggtaaaa agtgagctgc ccagcaattc acgtccgtta tacgaagtac tgatgaccgg
     5101 cgttccagcc tatgaaaatg gcatctatag taattaccat gctcagcgat ccaaagagtg
     5161 gtcgctgttt gaattagtta aggaagctgg cggaaaaaca ggagccgccg cttaccactg
     5221 gtacagcgag ttgtacaatc aagcccccta taatcgtttg gttgatcgca tccagcatca
     5281 ggaggagcga ctgattcagc atggcatctt ttatagtgag gacacttacc ctgattcgca
     5341 tttatttgcg gatgcccatt atttagtgaa tcagtatcag ccagactttt tggtcgtaca
     5401 ttcgatgaat attgatgatg tcggtcacaa atttaccgcc gattctcatg aatatgcagc
     5461 agcggttaat caggcggaca gattattggc tatttacctt cctgaatggc tgcaaaaagg
     5521 ttatcaagtc gtggtgactg cggatcacgg gatggatgcg cacggactgc atggcggcag
     5581 cttggctgca catcgtgaag ttccttttta tttgatttca aataaacgcc aaaagctgaa
     5641 ggaacaaact gtaccacagc tgctggcggc tccattgttt tgctggttat taggaattgc
     5701 accgtcagag aaaatgcagg acattcaaga attgatgagg ggatgaaaga gatgaaaaaa
     5761 cgcttggttc tggttcctgc ctcctttatc ttctgtgcat ttttactgct gccgctgctt
     5821 tttatggttc tgtgcagctt gcacagtgat ttaactaacc agtggacatt ggccaattac
     5881 agtgatattt tcagcaacaa atattacaca caggcctttg ttaacagcgt actgattgct
     5941 gtcagctcca gtgtgattgg gttaatcggg acgtttattt tatgtttatg cttaatgaat
     6001 ctatcgacta aattgcagga gaaaatcacc tttgtttcca atttggcagc taattttgct
     6061 gggattccgc tggcattctc ttttattatt ttgttgggga atgtaggtgt gctgaaaatg
     6121 attttgccat ttctagcgga cttcaatttg tattcttggt ggggattgac catcacgtat
     6181 acctatttcc aaattccgtt aggggtcttg tttttgtatc ccagcatgaa ggaagtgaaa
     6241 aaggaatggc tggagtccgc acaattgctg ggcgcttcat cagcgtatgc ttggttcaag
     6301 gtggctttgc cttttttgcg accatcgatt gccagcacct ttgtcatctt atttgctaat
     6361 ggcatgggga cctatgaaac agcgtatgcc ctgaccagca gtaatgtaaa tcttttaacg
     6421 attcgaattg cggctttagt gtcgggagat gtgttcgcca agcctaattt aggcagtgcg
     6481 ttggcagtcg ctttaggtgt cctgctggta acggcaatgt tgattattca aaagaaaggc
     6541 aaggtggtgc agacgtgaaa aagttaataa aaccgatttt gttctgcttt gtcctgtatt
     6601 acttgctgcc gattattgga acgattttgt atgccagctc cactaaatgg tcaaaatcgc
     6661 ttttacctag tgattttacc ttgcaatggt tccaacagct gctgacggac agggaattta
     6721 ttgctgcggt gggtcgctca cttttattag caggtgtggt cttggtgacg attctgttgt
     6781 tgatgattcc caccattatt tggattcatt tgtatttccc tcgtttgaat cgttggctgg
     6841 aaaagctgct cctgctgccg tatgctttac caggagtgat tttggtaacg gctttgcttc
     6901 gaacgtatgc tgaaactggt atcccaatgt ttgtagtgtt ggtaggtgca ttgtttatta
     6961 cggcgttgcc gattgtctat cttagtctta acaaccagat gcgcttaatt aatctgaagg
     7021 aattagtgga tgcagcagaa accttgggcg ccccgatgtc tacgattatt atccaggtgc
     7081 tgtttccaaa catccgtatc ggtgtgaccc tagtttctct gatgattttt tcttcggttt
     7141 ttggggagta catgctgacg aacctgttga ttggcggtcg atttcagacg gtacgcatct
     7201 atatgctgcg gcggatgaac gaaaacggcc atttggctag cgctgtgatg gttttgtatt
     7261 tgctgtttat ggtgggaatt gctttaacta cctttatgct gacttatcga caaaagcaac
     7321 agctgtccct taagcagacg agggaagaga agcagtcgaa aaagaagccc ttaaaggagt
     7381 tgtgacggaa aatgttttta gaaatacatg atctcaatct gttttttgat gaaaaacaga
     7441 ttttagctat tgatgaaatt cagattgaaa agggtgagct ggttacttta ctaggtccca
     7501 gcgggtgcgg aaagtctacg ttgctgcgct gtatcacggg attggaacaa ccaaagagcg
     7561 gtgagattgt gctggacaat gagaatattg ccgataagcc tacgaaggat cgcaatatcg
     7621 gctttgtttt tcaacagtat gcattgtttc caactatgac tgtttttgaa aatgtagcct
     7681 ttggtttgaa ggtaaagaag ctgagtcctg aggtgattca ggaaaaggtt tttgagatgc
     7741 tatcattggt ggatatgacg gagcaggcag ataaaaatgt ccagtttctt tctggcggtc
     7801 aacgacaacg ggtggcactg gctcgctcat tggtcactga accgaaagtc ctgctgctgg
     7861 atgagccatt aagtgctttg gatgcccgaa ttagaaaaca gctgcagcgg gatttgcgcg
     7921 ctatccagca atcgctaggg atgacgatga tttttgtgac tcatgaccaa gaagaggcga
     7981 tgcgaatcag tgatcggatt tttgtgatgg aagctgggcg ggtggctcag gtttccacct
     8041 cgaaggaatt gtatcagaat cctcagagcc gatttgttgc ggaatttatc ggcaactata
     8101 atcgtttcga atggtatgag ctgaatcagt atacccacga gttttccgct aagggcaatc
     8161 gctgcattta ttatttaaga ccggagctga ttcaatttga accggttcct cagggggtta
     8221 agattccggt tctatggaag gaatcattta ttttaggcaa cattcaacgg tatgtttttc
     8281 aaacgctgga gggcaaagaa attttagtgg atcggttgaa cgatcgggaa gagaaaatta
     8341 gcgatgcctt atacattcag ctgaaagata ttctagcaat ccctgtggaa attgatactc
     8401 agcattttgc acaacaaagc tagtgtatag tcggcaaaca tttctggctc tttgtcaaat
     8461 aggactgata gagtgatata agaacatcta aggtagaatt gattttctgc tttagatgtt
     8521 tttttatgtt taataagtga tcagatgatt gattaagaaa attctggttc tcatcctaac
     8581 ccgtaagcta ttcgtttcaa tgctttgatg tgggtatgca gattttctat tttgccattt
     8641 gagtaaggca actctaaggc taaacgaatg ccggattcat agtttataaa cgcttttttt
     8701 atatgatgca gttctcttgg aaccgacttg ggcattgttt ttacaagctg aaagaagatg
     8761 tccgcttctt tgtcataaaa agcttgaaga atttcttgat agtaagcata agctattcgt
     8821 agttcatcag aagtagaaag tagacgttct acgacttcac tttcggttaa ataggcccaa
     8881 ccaaaattac gccattttgt ccatttatca taaacaagcg tgtcctgtcg tttgatcaga
     8941 agtcgagata aagccttcac ttgtcgatag tgtttggctt gtattggatc gtttcggtcg
     9001 aagcttttca tgactcatag tcaaatatcc ttaaaactgc gaatgaggtg cttgacgatg
     9061 tgaaagcgat ccacaacgag tttggcatga ggaaagcact cttttatcag caaagcataa
     9121 gaggcgttca tatctgttac gagtattttt acgtttaaac gtgcacgtcg ttcaaattgc
     9181 ataaaataag aaatgagatc tttcttctta cgtgaaggaa gaatatccag taatttccca
     9241 gtatcgccat ccatacaaga aaaagaatag ggaccgactt gatttttcaa tgaacgaaat
     9301 tcatctacca atgaacagcg aggcaaccaa tttattttca cgtttgtcat ggggagcaga
     9361 gaagcgagtg ttcgcgaaac ggttgaccaa gaaacgccgc aatgtttaga aatcgtcgtc
     9421 attgcttgtt tttcagtcaa ttcctccaat attttataat gtacttgttt ggcgataaaa
     9481 cagttttttt caacaatgta ggtattcgcc gtaaaatagg tgtgacagcc acgacagagg
     9541 taccgttgct ttgccaaagc aagataagtc cgttgattcg cacacttatt gagaaggacc
     9601 agcgattttt tctttccgtt cttaacgatc agcccatgtt gattcacctg tttacactga
     9661 ggacagggct gaatcgtttt aatgagcgtg gcagaaacaa cgatactgtt gattgaatgt
     9721 atttttttct tctcgacatg agtgatttga atgtttccat ctttgattcc tagtaatagt
     9781 ttgataagat gaagatcagg catacagaac gctcctttga ttgtgttgtg gtgattcaat
     9841 tctaacggag cttctgtatg ttttgttttt ttatgctcaa aaggactgat agaatgattt
     9901 actatcagtc ctagaaatta tagcgccaca tttctttcac ctttgacttt tcagacggca
     9961 gtttttatgc tgatactgta gagagagcag gacacagctt ttgcttgttt tgggtaaggg
    10021 ggaaagaaga tgaccaaagc acttattttt gattttgatg ggacgatgat tgacagtgaa
    10081 tggatgtttt acgagagtat tcatcactat ttactgaagc ctatttactg gagcagttcg
    10141 gctataaaat tccttttgca gactatcaac aaggtgttgg gacggcagca gaactgtcct
    10201 tttatgaagg gataagtcaa gcggttggga gaccagtgga tgctgaacat tttgagcagc
    10261 ggatcagcag cactggaaaa tcggagtagt ctcaattctg aactgcggga gctagactat
    10321 ttttttgaag cacatccgat gattaaggaa aaaattgatt cctttgtgac gatcgatgat
    10381 cttcaggtcg gcaaaccaga tccagaaggt tatttgttgt gcttgaaacg gctgagggtt
    10441 gaacaggctg ttgtagttga ggatagtcca gtcggtgccc aagcagcacc gcatatgaca
    10501 gttgatgtac aagcgttgga tgcagatttt ttcgccttta gcgggcataa aatgtgcggg
    10561 ccgacaggaa tcggtgtatt atatggcaaa cgtcaattgt tagaacaaat ggagccagtc
    10621 gaattcggcg gtgaaatgat agattttgtc aatctttacg atagttcatg gaaagagctg
    10681 ccatggaaat ttgaagctgg tactccgaac attgcaggag caatcgatta cttgaatgag
    10741 attggtatgg agaatattca tcgttatgag caagaactgg ttgcctacgt tttacctaaa
    10801 ttacaagcga tcggcggtat caccatttac ggaccgcaag atcctgaaca tcatactggc
    10861 gtggttgctt ttaatttgga tggcatccat ccccgtgatg tggcgactgc tctggatatg
    10921 gaaggtgtcg ctgtacgttc aggacaccat tgtgcacaac ggttcatcga cgatttctgt
    10981 ttgactaatt ggtcgtttaa acagtcgatg ataatgccgt ttgagcggct ccaatgtcgt
    11041 ggaatctttg agtaagagtt tccaatatcg cttgagccga cggtacttct tctgattttc
    11101 agattacgtg attgattttc gtacagttct atgagaatcg cctgcttcag ttcctttgtt
    11161 agttgatgat gttcatcaac taagtccgtt ttcgcagaaa aggtctgccc acaatcatag
    11221 catagaaaac gggtacgttt taattctaaa atagtcaact tttctttgac tttcaacatc
    11281 tgagtacgtg tcgtatagga accgttgcgg ataatacgaa tcgatccaca gtgttcacaa
    11341 tgggaaggtg agtcatccaa tctcccttta ataaactgcg cttgaacgcc tttatattct
    11401 ttttcaatca gccattcttc atcaaaaaat aaatgtttat ctgttaatcc gagtaatttt
    11461 ctagtatgat tatccataga gcgactcctt tggtagggtg atgttttggt cgactaaatt
    11521 ttaccaaaag tgagtctctt tttgagttga aacgaagaaa aagtgttagt agaaaatcgc
    11581 cttcgatttt ccaacaacac aaaatattat agaagccttt tttctaaagg atttcaaggt
    11641 gtctgtttgc atttattaaa aaaaagaaaa agtattggca acagaaggcg tcaccaacac
    11701 tagatattat agagccataa tatgctgtat aatatctagt gtataggctt aagtggaggt
    11761 atatatgaaa atattaactg ttgaagatga taatatgata agagagggaa taagtgaata
    11821 tctttcagaa tttggatata ctgttattca agctaaagat ggaagagaag ccttgtcaaa
    11881 atttaatagc gatataaatt tggttatctt agacattcag atacctttta taaatggttt
    11941 agaagtgttg aaagaaatta gaaagaaaag caatttacca attctaatct tgactgcatt
    12001 tagtgatgaa gaatataaaa ttgatgcatt tactaattta gtagacggat atgtagaaaa
    12061 gccattttca ttgccggtct taaaagctag aatagattct ttaattaaga agaattatgg
    12121 acatttagag aagtttgaat ataaggatct atcggttaat tttaatagct atacagctaa
    12181 aataaatggt gaagaaatag atgtaaatgc a
//
LOCUS       DAFFSV010000093        12171 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFFSV010000093 DAFFSV010000000
VERSION     DAFFSV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.093"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(133..297)
                     /locus_tag="LR110_002125"
     CDS             complement(133..297)
                     /locus_tag="LR110_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823075.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            592..1920
                     /locus_tag="LR110_002126"
     CDS             592..1920
                     /locus_tag="LR110_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBL4823076.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="LR110_002127"
     CDS             2122..2955
                     /locus_tag="LR110_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBL4823077.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="LR110_002128"
     CDS             2971..3708
                     /locus_tag="LR110_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBL4823078.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="LR110_002129"
     CDS             complement(3863..4831)
                     /locus_tag="LR110_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBL4823079.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="LR110_002130"
     CDS             5063..5896
                     /locus_tag="LR110_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBL4823080.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="LR110_002131"
     CDS             5930..6769
                     /locus_tag="LR110_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4823081.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="LR110_002132"
     CDS             6795..7355
                     /locus_tag="LR110_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL4823082.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="LR110_002133"
     CDS             7554..9275
                     /locus_tag="LR110_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBL4823083.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="LR110_002134"
     CDS             9440..10582
                     /locus_tag="LR110_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL4823084.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="LR110_002135"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="LR110_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBL4823085.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4148 a         2042 c         2527 g         3454 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DAFFSV010000094        11898 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFFSV010000094 DAFFSV010000000
VERSION     DAFFSV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.094"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LR110_002136"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LR110_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBL4823086.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LR110_002137"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LR110_002137"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBL4823087.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="LR110_002138"
     CDS             complement(2169..2906)
                     /locus_tag="LR110_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL4823088.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="LR110_002139"
     CDS             complement(2997..3524)
                     /locus_tag="LR110_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823089.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="LR110_002140"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="LR110_002140"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBL4823090.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="LR110_002141"
     CDS             complement(4401..4886)
                     /locus_tag="LR110_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBL4823091.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="LR110_002142"
     CDS             complement(5039..5236)
                     /locus_tag="LR110_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823092.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LR110_002143"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LR110_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBL4823093.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LR110_002144"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LR110_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBL4823094.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="LR110_002145"
     CDS             9211..9423
                     /locus_tag="LR110_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBL4823095.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="LR110_002146"
     CDS             9425..11104
                     /locus_tag="LR110_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL4823096.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALREYLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="LR110_002147"
     CDS             complement(11358..11558)
                     /locus_tag="LR110_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL4823097.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..>11898)
                     /locus_tag="LR110_002148"
     CDS             complement(11708..>11898)
                     /locus_tag="LR110_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HBL4823098.1"
                     /translation="YFYTCTNLYYPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLI
                     IGRKRYFSLREEGMMEEK"
BASE COUNT         3447 a         2570 c         2070 g         3811 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat atttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatc
//
LOCUS       DAFFSV010000095        11792 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFFSV010000095 DAFFSV010000000
VERSION     DAFFSV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.095"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(193..678)
                     /locus_tag="LR110_002149"
     CDS             complement(193..678)
                     /locus_tag="LR110_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBL4823099.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    768..1113
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1158..1799
                     /locus_tag="LR110_002150"
     CDS             1158..1799
                     /locus_tag="LR110_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL4823100.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1818..2453
                     /locus_tag="LR110_002151"
     CDS             1818..2453
                     /locus_tag="LR110_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL4823101.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2471..3286
                     /locus_tag="LR110_002152"
     CDS             2471..3286
                     /locus_tag="LR110_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4823102.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3605..5041
                     /locus_tag="LR110_002153"
     CDS             3605..5041
                     /locus_tag="LR110_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL4823103.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5230..7677
                     /locus_tag="LR110_002154"
     CDS             5230..7677
                     /locus_tag="LR110_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL4823104.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7907..8974
                     /gene="rlmN"
                     /locus_tag="LR110_002155"
     CDS             7907..8974
                     /gene="rlmN"
                     /locus_tag="LR110_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL4823105.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9167..10270)
                     /locus_tag="LR110_002156"
     CDS             complement(9167..10270)
                     /locus_tag="LR110_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL4823106.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10823..11371
                     /gene="aac(6')"
                     /locus_tag="LR110_002157"
     CDS             10823..11371
                     /gene="aac(6')"
                     /locus_tag="LR110_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL4823107.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3907 a         1986 c         2532 g         3367 t
ORIGIN      
        1 ttttatatat gcttcttttt tcgtagaatc aataaatatg gatttggcta actatggaag
       61 aatactcgta ttagttttca gcgttcatac tagttgttac attgtttgat tggccaaaag
      121 aatttctgat atattttgtg ctaaataaag aaaaagacgc ttgaattggg aaataagaaa
      181 actactctat cattacaaat tttcataaac tggcagatgt tctaagtaaa caagtatctt
      241 ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg atatttggat gaaagcctac
      301 ggtatcttga cgatcaatag cagcatcgat cacgacttgg atttttttct ctttgtcgtg
      361 catcaatgct aatggagtca ctgtacctgc tggaacgcct aggaggtcag gaagctgttc
      421 ttcagaaaca aaagataagc gcttttcttt taattgttca gctagttgtt ttaaatcggc
      481 gtgcttttca tcaggcaaga tgacaagata gatttgtttt ccttttcttg atttcaataa
      541 tagatttttt acttgcggac caggtaggga aaaagggaca tttttgactg acgtaatcgc
      601 aggatgatcg actttttgaa aagagatacc cagttttttt agtagttcat aagcttcttg
      661 ttcggttgca cgactcatcg cgcatcaccc tccttttttt attagtgtat cataaaaaag
      721 agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa atgacatcga
      781 aaagaagagt agccgttagg tgtttttaga gagtctctgg ttggtggaaa gaggcagcag
      841 ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg tatagtagga aaatccgggg
      901 agcccgttac agctgcggtc gttgctagat gaccagataa ggctggcttt tccgtgagga
      961 gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga tcaaaggact
     1021 ttttgttttc atctgaacgg caacgactgt gtgaggtttt ttctgcaagg aaaaaataaa
     1081 cagaggtgga accacgtata atacgtcctc ttggcatttg ccaagaggat ttttttattt
     1141 taaaggagga aaagaagatg gagtatttga ttgaagttat gccgcaatta ttagtaggag
     1201 cactcacaac attgaaatta tttttattta ccttgattgg ctcattacca ttagggattg
     1261 ttttagcatt aggattgtct ttgcatttta agccgctacg ctacttgctt caaacatatg
     1321 tatgggttat gcggggaaca ccgctgttgt tgcaactgat ttttattttt tatgggttgc
     1381 cgacgattgg gatcgttttt gatcgtttcg aagcagcact cattgccttt atcttaaact
     1441 atgcagctta ttttgcagag atctttcgtg gagggataca atcgattcct caaggtcaat
     1501 atgaagcagc gcaagttttg caattaagtc cctttcaaac ggtgacgaaa atcgtcatcc
     1561 cacaagtgac aaagattgtt ttaccttctg tagggaatga agttatcaac ttggtcaaag
     1621 attcttcctt gatctatata ttaggtttag gtgacgtaat gcgtgctggg aaaatcgcga
     1681 tggaacgcga cgcgacttta ctgcctttgc taggcgtggc aatcatctat cttacattga
     1741 ctggtattct aacaatcata atgaaacaag ttgaaggaaa attaaattat taccgctaac
     1801 tgataggagg aagaaatatg attgaattga agaaagtgaa caaagcattt ggtgaaaaaa
     1861 aaataatcaa agatttgaat ctaatcattc cagatgggaa aacattagct atcgtaggtc
     1921 cttccggtgg cggtaaaacc acgcttttac gtatcttggc tggcttagaa agttctgaca
     1981 gcggagaatt ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa atgaaagaac
     2041 aagttgtagg agtcgtattc caagacttcc agctatttcc acatctgaat atttttgaaa
     2101 acattacctt agcaccaaaa atggtattga aacagacaaa agaagaatac accaaaaaag
     2161 cagaagaatt agcggcttca ttagggatta gtgatttatt gaatatctat ccttatcagc
     2221 tttctggcgg acaaaaacag cgggtagcga ttgccagagc attggctatg aatccacggg
     2281 tattggctta tgatgaacca acaagtgctt tagatcctga attacgccag caagtcgagc
     2341 gtttgatcct atcattgaaa gaagatgggg taacccaaat tattgtcacc catgatttag
     2401 tatttgcaga aaatgtcggt gatcaattac taaaagtttc cccaattcat taagaaagga
     2461 gaagatagag atgaaaaaaa ccagttttct attcgctttg atcgcaggtc ttcttctttt
     2521 tgcaggctgt tcgaacaaaa aaacttcagc agataattgg gcgactatca acgaagagaa
     2581 aaaaatcgtt ataggtttag acgatacatt tgttcctatg ggatttagag ataaagacgg
     2641 gaagctcaca ggatttgata ttgatttagc caaagcagtc tttgaacaat atgggattac
     2701 ggtagacttt cagcccattg actggtcaat gaaggaattt gaactgaaca atggaacgat
     2761 cgatttgata tggaacggct attccaaaac gccggcaaga gaaaagaaag tgcaatttac
     2821 aaaaccttat atggaaaacg atcaagtgct gatcacacct aaagaatcag gaatcacaaa
     2881 ctttggacaa atgaataata agagattagg tgcgcaaaat gggtcatctg gttatgatgt
     2941 atttacgaaa caaccagaag tattaaaaga gatagtcaaa aaccaagatg ctgttttgta
     3001 tgatagtttt aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg gtctgctgat
     3061 ggataaagta tacgcagact attatttgaa acaaagaaac gaactagaag acttcaacat
     3121 aacaaaaggt tcttatgaga gtgaggattt tgcagttggt gcacgcaagt cagatagtac
     3181 attagtgaaa aaaatcaatg aagccctaga tcaactagag aaaacaggtg aattccaaaa
     3241 aatatccgat aaatggtttg gcgaagatgt tacgccgaga aattaacaaa taggggactg
     3301 tgacaaaaga cgtgtcacag tccctttttc cgaataaaca gtgttctcct gccaactcct
     3361 tgtgactcgg agctgaatgg caggctcaca acctcttggt aaacggtgtt caaaagccag
     3421 tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt
     3481 cttactactc gaaactaagc gactttttca caacctcctt tacataattt tattttgttg
     3541 tggaaggggt ttcgtgttat tctaaaagag gaacgattga aggttatagg aaaggaagag
     3601 atgaatggaa cacaaagaac ttacctcatt tccaaaagat tttctgtggg gctctgcttc
     3661 tgcagcgtat caagtagaag gagcttggca agaagatggg aaaggagaat cagtctggga
     3721 tcgatttgta cggatacctg gaaagacatt taaaggaacg aacggtgatt tggcagtaga
     3781 ccactatcat cgttataaag aagatattgc tttaatgaaa gaacaaggtc tgaaagctta
     3841 ccgcttctct gtagcttgga cacggatttt tcctaatgga cgaggagaag tcaatcaagc
     3901 tgggctcgct ttttatgaga gattgatcga cgaattgatt gaaaacgata ttgaacctat
     3961 gttgacttta tatcattggg atcttccaca agcattgcaa gatgaatata atggttggga
     4021 atcacgtcag atcattgaag atttcactca ttatgcagaa acattatttg aggcattccg
     4081 cgggaaagtc cattactgga ttagtttgaa tgaacaaaat atcttcactt cattaggtta
     4141 tttgttagca gcacatccgc caggtgtgac agacccgaaa cgaatgtatg aagttaacca
     4201 tattgctaat ttagcaaatg catctgtcat caataaattt cacgagttag aaatgcctgg
     4261 aaaaatcgga ccaagctttg cgtatacgcc aaattatcca attgacagta atcctgaaaa
     4321 tgttctggct gctgaaaatg cagaagacct gatggccaat tattggatgg atgtctatat
     4381 gtggggaaaa tatccgattg ctgccatgag gttcttagaa gaaaaaggat gggcaccaac
     4441 gattgaagct ggcgatgcag aattgctaga atctgctaaa ccagattttc ttgggatcaa
     4501 ttactatcaa acggcgacga atgcattcaa tccattaaac ggagttggtg ctggaaaaat
     4561 gaatacgaca ggaaaaaaag gttcatcaga agaaactgga gttcctggaa tgtataaaaa
     4621 agttgagaat ccatttgtcg aacgaacaaa ttgggactgg gagattgacc cagaaggatt
     4681 aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga ttacggaaaa
     4741 tggcttagga gagtatgaca agttgacaga agataaacaa atccatgacg actatcgaat
     4801 caattactta caaagccacg tcaaagccat caaagaagca atttcggatg gagcagaagt
     4861 cctaggctat tgcacctggt cttatacaga tttgcttagc tggctgaacg gttatcaaaa
     4921 gcgatatgga tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt tggaacgata
     4981 taagaaagac agttattact ggtaccaaaa actgattgaa gaaaatggag agaaccttta
     5041 gttcacatac ttaaaacaag aaggtacggc tggatatggc cgtgcctttt cttgataaaa
     5101 tcatttagaa attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa gcgtcagttc
     5161 tttcaaacaa aactttagtt cccttacttt tttttgtata atgaaacttg atgaaatgag
     5221 ggataataaa tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac gaaaaaaacg
     5281 aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg gtgttgggtt tgccttgatt
     5341 ttatttgctg tcttcggctt tttaaaatta ggttttttag gaatattgat tgcaaacggc
     5401 tttcggatca ttgcaggaaa tacttaccaa atcctctgtc ttttattagc cgtattagga
     5461 ttttggattg tcattaagaa tacagagttt tcaattggta aaaaccgacg ctggtttggc
     5521 ggcatactct tttatttcgg tatcttatta ttgttacatg cccatttatt tggcaaactc
     5581 catactgggg aaccaaatat catggggacg acatgggatt tgttagccag tgatataaaa
     5641 caaagtcaag tagacaataa cgttggtggt gggatgattg gagcaatcct ttatcatttt
     5701 acttacttct tgattgcaca gcctggaagt tatttagcag cagtgctatt actggcagga
     5761 ggagcattcc tgttcagtaa tctggaaggc tatcagctgt taaatggtat ccaatcaatt
     5821 ggagaacgag tacaggaatt attggaaggt gatcctgcca agcaagctcg aaagcaagcg
     5881 gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg cttagctgca
     5941 aaagaagctg cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt gagccagcaa
     6001 agggaaaata ctgctgatga atggcaggaa ccagaaaata cagagccgga gcagctaagt
     6061 tttgttccaa tcgacagttt tcaagaaaat atccatcctg cgaatctaga gaaaccagta
     6121 ccagatacac caaaacagac caatacagca gaaggttttt cagatgagtt gccagaggat
     6181 gacggtacct cattagaatt tgagattgaa gcagaacagg aaaatcaaga ctatgaatta
     6241 ccgacagttg atttgcttga ttctatacca actgtggatc aaagtgatga gtataaaaaa
     6301 attgaaaaaa atatcggcgt attagaacaa actttccaaa gtttcggtgt cgatgccaag
     6361 gtcgtcaaag ccagcttagg accatccgtc acaaaattcg aagtccagcc ggctgtaggg
     6421 gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt agcggcgaaa
     6481 gatgtccgga tggaagcacc gattccaggt aaatcactga ttggtattga agtaccaaat
     6541 ggaaagatca gtatggtttc ctttagagaa atcatcgaag cacaaccaaa ccacccagat
     6601 aaattactag aagtaccttt aggacgtgat gtttctggac gggtacaaac ggctgatctt
     6661 tcgaagatgc ctcacttact cgtggctggt tcgactggta gtgggaagtc agtagcaatt
     6721 aatgggatta tcacaagtat attaatgcgt gctaagcccc atgaagtaaa actgatgatg
     6781 atcgatccga aaatggttga attgaatatg tacaacggca tacctcatct gttgacgccg
     6841 gtcgtgacga accctagaaa agcagcgcaa gccttacaaa aagtagtaca agaaatggaa
     6901 gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata tttctggtta caatgaattt
     6961 gttcaacaga aaaacttgga aaacggaacg aaacatccaa ccttaccatt catcgtagtg
     7021 atcgtagatg aattagctga tttgatgatg gtagccagca atgaagttga agatgcgatt
     7081 attcgtttag cgcaaatggc tcgggcagcg gggattcata tgatcctagc tactcaacgt
     7141 ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg taccttccag aatggcattt
     7201 gcggtatcaa gcggtacaga ttctcggacg atcattgatt ctaatggggc agaaaagctc
     7261 ttagggcgag gagatatgct attcttgcca atgggtgaga ataaaccgat ccgtgtgcaa
     7321 ggagcattca tttctgacca cgaagtagaa cgggtcgttc aatttgttac agatcagcaa
     7381 gaagctcatt atgaagagaa aatgatgccg acagatgaag ttgaaacgac aggtgctcca
     7441 gaacagccac aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga aatgcaaacg
     7501 gcaagtattt cccttctcca aagaagattt cggatcggtt acaaccgagc agcacgttta
     7561 gtagatgaac tggaagctca tggagttgtt ggcccgtcag aaggaagcaa gcctcggaaa
     7621 gtattcattg agcaggaaga aacatcagaa acgttgccgg acgataatga catgtaaaac
     7681 ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa gacaaggcat gatgatacca
     7741 aaagaaaaaa tggttttttt ccatcttttc taatacggta tgatgtcttg tctttttagt
     7801 ttttgtgaga ttaaagttct attcgattta tttgtgaagt attgcattaa ggaaatgaat
     7861 catgtatagt atgtagcgaa actttgagaa aaagagggaa aatactttgg aaaaacagtc
     7921 catttatggc ttgacgaatg aagaattaat caattggttt atagaaaacg gcgagaaaaa
     7981 attccgagct gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta atttcacaga
     8041 aatgacgaat ctatcgaaac aattgatcga aaaattatcg gagcatttta tcatcaatcc
     8101 tctaaaacag atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt tgtttgaact
     8161 ccctgataaa aacatgattg agactgtact gatgagacaa gaatatggct tgtcagtttg
     8221 tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg ggctgctcaa
     8281 aaagaaccgt gatctgactg ctggagaaat tgttgcacag atcatgatgg tccaacatta
     8341 ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg ggatcggtga
     8401 gccatttgat aattacgaca atgtaatgga ctttttgcat atcatcaatg atgccaaagg
     8461 attggcaatt ggtgctcggc acattacagt ttccactagt ggattagcac acaaaatcaa
     8521 ggaattcgct aataacggct tgcaagtcaa tctggctatt tccttgcacg cgccgaataa
     8581 tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc ccaattgaga aattgatgga
     8641 agctgtggat gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat atatcatgtt
     8701 gaatcaagta aatgatagac cagagcatgc gcaacaatta gcagatctat taaaagataa
     8761 gaaaaaatta gcatatgtca acttgattcc atataatcca gtcagtgaac atgatcaata
     8821 ttctcgcagt ccaaaagctg atgtattgag attttatgat gtattgaaga aaaacggcgt
     8881 aaactgtgtg atacgtaaag aacatggtac agatattgat gcagcttgcg gacaattgcg
     8941 aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt ttttcaatta
     9001 ccataaaagg agcagttttc ttaattaagg aactgttcaa cttaaataag agaaaagatt
     9061 ctatagcgaa agctataaag ctttttttga caagaaaaac cctcatcaag caaagaaatg
     9121 tcctcaattt gatgacagcc tgcttgatgg gggttttagc aattatttaa ctgattttct
     9181 tatatccttg ctggctttct tgtgtagcat gataagtttc tacgtcaaga atggcctgtt
     9241 taaatttttc tcgggtgatt tcataaggca catgtttaat atcatttgtt gccatggctt
     9301 tatctaagat tgcttctaaa tcggtcgtga cattgagatt catgtcggtt aacgatactg
     9361 gaagttggtt ctttttcata aacacataga ttttatcaaa caaatcgaac tgctgatcca
     9421 tcatcaataa ggtcagtaca ccataactga cagatgctcc gtgaaggtat ccctccattt
     9481 tttctagaac agtcgtaccg taatagaatg agtgtgccat agcagaatta tagttattgt
     9541 taactaaaac agaaaccaat ccggtagtaa caatgatatc cgaaacgact tgttcaattg
     9601 catatgaggg acgatcgtcg gcactatctt tcaatgcttg cgtaccataa gtcaagagac
     9661 gatcaatgca catttttccg atttggattc ctaatctatc cgtgtagcct agatcatcgc
     9721 cacgagcaga aaaagtagat tcgacttctt tggataaagc atcgccaata cctgcccata
     9781 agtatttatc aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt acaggtggtt
     9841 ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt atagatgaca cataccgcag
     9901 tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa gactggttta gctagttttt
     9961 caccaatgac ttttacagta tcgcaagcac ggccaccacc gactgcaaac aggacgtctg
    10021 ctttttggac actttcatgc tggatcaact gttcaatatt tccataagtt gaattacccc
    10081 cgtaccagag actggcgaca acctctattt cctgtgtcaa agctactttt attttgtttt
    10141 ctgcctttga aagtgcagtc ttccctccaa tcagcactac ttttgtccca tattctttga
    10201 cgattccatt tgctttttca tacgcatctg ttccaatggt ataatttggt aagaaaacac
    10261 tttgactcat attcatttcc ttctttcttc gttgaaacgg gttaaaattt taaagagtga
    10321 tcgatctgac tgcggctgta gatggagtag aggtgaacct aagacaggat aagtgatgaa
    10381 taaggtggca tgaacacctt gaaatcatca ataagtatcc taaaaacgaa caagtacaaa
    10441 aacattgaaa acgacaataa tgaatttttg ttcctcttag tatttctacc gattcccttt
    10501 gcttttttgt aattatctaa aaattctaaa aattaagatt attatatgaa aacaggtttt
    10561 atttgtcaat tcataattgg ttttttttaa agcaaaagat aatctatatc atgatgaaca
    10621 aacagagttt tttcctccaa ttgatggctt tcctgatctt tcctattttg aataaagtgt
    10681 ttctgtataa tttttttatt ttataagtaa gataaataat gaaagaatag agatactgat
    10741 tggtagctaa agatttttga gcgagaacat ttcgtttttt caaaaggact gttagaataa
    10801 gcaagaacaa gggggaagac gtatgataat cagtgaattt gaccgtaata atccagtatt
    10861 gaaagatcag ctttctgatt tactgagact gacttggccg gaagaatatg gagacagctc
    10921 ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag cggtagacca
    10981 agatgagtta gtaggattta ttggtgcaat ccctcaatac ggtatcacag gttgggaatt
    11041 gcatccatta gttgtagaaa gctcccgacg aaagaaccaa ataggtactc gattagtcaa
    11101 ttacttagaa aaagaagtag cttccagagg aggaatcacg atttatttag gtacggatga
    11161 tttagaccat ggaacaacgt taagtcaaac ggacctgtat gagcatacat ttgataaagt
    11221 ggcttccatc cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat taggttataa
    11281 aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa
    11341 aacgattatt cctcgaccaa attctcaata ataaaaagag gttgtgacag aagtaatcag
    11401 cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg aatttttgtg aaattccaac
    11461 ttatttccga agaagctact tttggaacac cgtgtaccag aggttgtgaa aaagtcgctt
    11521 agtttcgagt agtaagaaaa attttgtgaa aatggctttc tacatttttg cataaatttg
    11581 gcttaatacc gagaaactgg cttttgaaca ccgtttattc agataaggga ctgtgacaag
    11641 acttttgtca cagtttcttt ttgtttattc ttttttatct ttgtatggca ataatagaca
    11701 atataacgaa aattatttaa tggcttttgg aagaccatgg aaaaccttgt acaaagctat
    11761 tttttctgaa aaattccttg aaacctcacg aa
//
LOCUS       DAFFSV010000096        11356 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFFSV010000096 DAFFSV010000000
VERSION     DAFFSV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.096"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(73..1413)
                     /locus_tag="LR110_002158"
     CDS             complement(73..1413)
                     /locus_tag="LR110_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL4823108.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(1580..3700)
                     /locus_tag="LR110_002159"
     CDS             complement(1580..3700)
                     /locus_tag="LR110_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL4823109.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            3930..5108
                     /locus_tag="LR110_002160"
     CDS             3930..5108
                     /locus_tag="LR110_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBL4823110.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            5124..5882
                     /gene="aroD"
                     /locus_tag="LR110_002161"
     CDS             5124..5882
                     /gene="aroD"
                     /locus_tag="LR110_002161"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBL4823111.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            5905..6390
                     /gene="ybaK"
                     /locus_tag="LR110_002162"
     CDS             5905..6390
                     /gene="ybaK"
                     /locus_tag="LR110_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBL4823112.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(6461..7714)
                     /locus_tag="LR110_002163"
     CDS             complement(6461..7714)
                     /locus_tag="LR110_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL4823113.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            7831..9180
                     /gene="gdhA"
                     /locus_tag="LR110_002164"
     CDS             7831..9180
                     /gene="gdhA"
                     /locus_tag="LR110_002164"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBL4823114.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            9292..10638
                     /locus_tag="LR110_002165"
     CDS             9292..10638
                     /locus_tag="LR110_002165"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBL4823115.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(10946..11332)
                     /locus_tag="LR110_002166"
     CDS             complement(10946..11332)
                     /locus_tag="LR110_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL4823116.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3551 a         2081 c         2349 g         3375 t
ORIGIN      
        1 ctgaaattag aggctcttcg tcaatgagtg ttggtgaaga aattcaatag aataatcgag
       61 atactcacac ggctagacag ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag
      121 ttgggtagtg ttcttagttt tcttgaaata ttttcctcga tataaaaaga ttcgttctcg
      181 gtagttttga aagtttctaa agccataggc aattcgtttt agtactttga tatggttgtt
      241 caaacattct atagggccgt tggaataggg aactcgcaac gctcgtttga tttctgttcg
      301 atatttcttg aaggtcgtga acactggttg atagtgctct ggcaactcct tgtagtcttc
      361 ttttaggagc gcgacgaatt caggaacatc ttgtcttctg atagcggaaa gaaaaacctg
      421 gtagacttcg tagcctcgct ttaaggagtc atcataatat aatagccgat cgactatctc
      481 cgcttctgtc agatgatctc gaaatgacgg acgccagtag cgatgctgac cattcaattc
      541 ccacgcattc ttttgaagta gtctccagta tttctttaag tgcttcgacc gacgatcccc
      601 ttttttaaag gtcttcatta cttgaattcg gtggttggta aaggcccgac cgatgtgttg
      661 gctaatatga aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt
      721 gcgatagggt gtatagaaat ctgaaaccac tagacgtact tgttcacgga cttttctagg
      781 gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag
      841 ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct cccgagacgt ttttcaccga
      901 tttgaattca tcgaaacaca ccacttctgg taacggctgg ttcaaagaaa cggttttagg
      961 ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcaat
     1021 caaagacata gaaataggct cacttaatag ctcaagaatg gcttgtttga ctctgcgggc
     1081 aatggagcag cgacgatcgc taactgaatc ttctgctaaa aaagtctttc cacagtgttt
     1141 acatttgaag cgctgtttgt taatccgcac aatggtcttg tagttactga catcattcaa
     1201 taagatggat acttttttcc agccccattt aatgatcgtt tgcttatttt gatagtggca
     1261 atggaagcat tcctctggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc
     1321 attaatgcgc tctttgcgaa aggcatcttc atgaaaggta agatttaggt ctagaatatc
     1381 tagtaactct ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc
     1441 gtcgacttta ttctaaagga tttcaaggtg tctgtttgcg tttattagaa aaaagaaaaa
     1501 gtattggcaa cagaaggcgt caccaacact agatattata gatcctaatt ttgtcgctgc
     1561 tccttttaaa tttattttat cattcggaag aacttgtggt cgttccgtga agttcttggt
     1621 acgtttgcgt tttataaaga tcaacagttg attgtccgtt gtgttcacta aagacactag
     1681 ttgacttgtc accaagagat ttttcagctt cttttaattt ctctaagcca ttcttgtagt
     1741 cataatcact ggtatcaatt gggtctaatc cactattcgt atagaaccga agaagatcac
     1801 cattattgat ttggtcagaa acagctaact gtttatcaac tttttctttc agtgaatcaa
     1861 tttcattctg tacttcctca gtaggttcag taatagcttc accagtttcg ttactataga
     1921 tcgtaccacc gtagtaagta tattttggtg taacaaaatc accatcacgg aaagaaacaa
     1981 gctgttcatg atctttcgag aatagatctt gtcctaactg aatataattt tttgtatcta
     2041 cacctagtaa gtgaagtaat gtaggcaagg catcaacttg tccgccgtaa gtgtgattga
     2101 ttccgccctg tgtttgtcca gggatatgga tcatataagg gacacgctgc atttgtgcat
     2161 tgtcgtagtt tgtccaagta gaacttgttt ttccaactaa ttcagctaga tttttatttc
     2221 ttgaattcga gacaccgtaa tggtcaccgt aaaggacgat gactgaatta tcatacaagc
     2281 cgctttcttt caaataattg aagaattctt gtacggcggt atctaaataa ttggctgtcg
     2341 caaagtaacc gttgatcgtt tcatcatttg tattggctaa cgggaatcct gcttcgtcat
     2401 tggtgaattt tgaataagga taatggttag atacagcaat gaattttgaa taaaatggtt
     2461 gttgcagacg ttccaagtat tgtacggatt gttggaaaaa tggtttatca tgcaacccgt
     2521 attggaacga attgtcatca ttaacatcat aataagaaga atcaaagaaa taatcatatc
     2581 ctaaattctt gtatgtctcg tttcgattcc agaatgtacc agcgttccca tggaataccg
     2641 cactaatgta accttgtgtt tgacttaaaa tatcaggtgc agcttggaaa gtattttttc
     2701 cgcctaattg tgtgaacaag gagccttgat tcaagccaaa caatgaattt tccatcagcg
     2761 tttccgcatc gcttgttttt cctgctttta cttgatggaa aaagttgtca aaactgaacg
     2821 tgctgttgct gtggtaaaga ctattgatga aaggtgtaac ttcatgctgg agaccattct
     2881 catctgtcaa tttataatcg atcaggaatt gctggaagct ttctaaatgg atataaatca
     2941 cattttttcc tctagcgagc ccgaacaaat cgtcatttgg tgctgcataa tgttctttta
     3001 cataattttc tacttctttc atatcattgg gacttgcttc tgcccgcact tggtttgtct
     3061 ggtaagtttg aacaccgtca taaaccatga aagcattcaa gccaagatat ttgaccaaat
     3121 aatcacgaga gaatgttcga gtcagcaatt ctggacgatc agcttccgcc ataaaaagat
     3181 ttcctgagaa aatcataact gctaatgtag agatcgcaaa agccattctt gctcggatcg
     3241 ggcgagggtc caacttgatt ttttttgcaa tcagcagaac agtcaagata ataaaatcga
     3301 tccaatagat cacatcatat gggcgaaaca gtcgaatggc actttctccc agaccactcg
     3361 ctactttccc agcacctaaa atcgtgttga tcgtaatgaa gtccgtgaat tctcgataat
     3421 aagtgacatt cgaaaaaaca agcaatgtca aaagaaaata aatgaccatc atcgtgatat
     3481 aagcaactct agggcgtctg atataaagag caattccaag taataacaat gtagttgcaa
     3541 tagggttgat aaacaagata aagtattgaa tgccactttc gattccgaga tggaaatcaa
     3601 ctaaataggc aaaaatcgtt ttcaaccaaa gtaaaacgac tagtaaagaa aaaaatccga
     3661 gcctcttatt caaaaaggca ggtgttttta tacatttcaa gataatcttc ctttctgcac
     3721 ttcttaatca cagaagtgtc ctgaaaccat gttacacagc tcacttattc tcatttattt
     3781 tactagtatg acaatatagt gtcaattgaa accagaagtt tatgctgttt cattaaggtt
     3841 ttctttaagc ttcatgaaaa aatgagcagt aagacaagtc ctatgttttt tgttatactt
     3901 gggaagagaa agctaaagca ggtgaaggaa tgaaagtaaa agtaaaaaaa agagcaagca
     3961 atcgattgaa aaaaggatat ccactgatcc aaaaagaaga ccttgctcat ccccaaaaag
     4021 aaaccacgca atgggtggaa ttagttgatt cgcaaaacca atttatcgca aaaggatatc
     4081 taggaaagca gaataaggga atcggttggg tattgagcca gcagaacatc ccatttgatc
     4141 aatctttttt tgaagaattt tttgtgaagt caaaagaaaa aagaactttc tttttttcag
     4201 atgattctac taccgctttt cgtatattca atggagaagg agacgggatc ggcggtctga
     4261 ttgttgatta ttatgcaggt tatgctgttt tttcttggta taacgaaacg ttatatgcac
     4321 atcgtgaatt attgattcaa gcttttcgtg aaatctaccc ggaaatcata ggaggctatg
     4381 agaaaattcg atttgaatca gtaaatctgc ctgagagcca gttcatttac ggaaaagagg
     4441 caccggaacc cttgatcgta aaagaaaacg gagtaaaatt tgccgtttat ttaaatgaag
     4501 gattaatgac tgggatcttt ttagaccaaa aagaagtacg gggatcatta gcagaaggtt
     4561 tcgcttctgg gaaaacagtt ttgaacatgt ttagttatac cggcgccttt tctgttgctg
     4621 ccgcaatggg aggggcaatc gaaacaacaa gtgtcgatct agcaaaacgc agtttaaaaa
     4681 aaacacagga acagtttgaa gtgaatggtt tggatgtcga tcagcaaaaa atcattgtta
     4741 tggacgtatt cgagtatttc aaatacgcca aaagaaaaca atttttatat gacatgattg
     4801 tattggatcc accaagtttt gccagaaata agaaaaaagt ctttcgcgta gcaaaagatt
     4861 atggggaact ggtcaaggat agtctggata ttttagcaga agacgggata cttattgcgt
     4921 ctacaaatgc tgcgaatgtc tcgatagaac aattccaaac aatgattgaa gaggaattcc
     4981 atgccaaaaa tgtttctttt gaacaacttg ctttatatcg tctgccaaaa gatttccaga
     5041 cgacggatac ctttttagaa ggcaattatc tgaaagtgtt tgtttatcaa attaaaaata
     5101 accattagaa ggagcgatgc agaatggttc gggtaaaggg attgaatata ggaagcggta
     5161 gaccaaagat ttgtgcgcct cttatcgggg ccgtccgaga ggaagtatta cgtgaagcag
     5221 ttcttgcacg tgaagcaggg gccgatttag tcgaatggcg agtcgatcat tatcgtgaag
     5281 ttttttcttt tgatgaagta gcagaaacat tggttcttat ccatgagatg cttgatggac
     5341 tgccgttatt atttacgttt cgtaccttag ctgaaggcgg agaacaagac atatcgatcc
     5401 gtcagtatcg cgacctttat gaacatgtga tacacacagg attagtagat atggtcgata
     5461 tcgagttgtt caaagtagaa agtctaggaa aaagactgat agaggaaatc aagtctttaa
     5521 acattccttt aattatcagt agtcatgatt ttaaagaaac accagcagac cctgtcttgc
     5581 tttaccgatt gaatgttatg gaacattttg gagccgatat cggaaaactg gcagtaactc
     5641 ccaataatga acgagatgtt ttgcggttga tggagttgac tagacgggca aatgcttttt
     5701 tctccatgcc tattattacg atgtccatgg gaaatcttgg taaaatttct cgtctggttg
     5761 gcggaacgac gggttccgtt atgacatttg gttccttaag cagcgattct acttctgctc
     5821 caggacaatt accggtcaaa gcattgaagc aaattattca attattaaat gaaccgttgt
     5881 agaacaagtt tgaggtgata ttttttggca aagaaaaaaa tagtaaaaac gaatgcaatc
     5941 cgtatggtcg aacaaaaaaa gattccctat acagagcacg aatatgagtg ggacgaaagc
     6001 cacctaagtg cgtcatcggt cgctgaacag cttccagaaa gccaatctcg tatatttaaa
     6061 acactggttg ctgtgggcaa tgtgacgggg cctcttgttg ctgtgatacc gggagaagcc
     6121 gaattgaatc taaaaaaatt agcaaaagtt tcagggaata aaaaagtaga aatgctccat
     6181 ttaaaagatt tggaagcaac gaccggatac atacgtggtg gctgctcgcc tataggtatg
     6241 aaaaaactat ttcctactta tttagatcaa attgcagaaa gctatgaaca aatcattgtc
     6301 tcggctggca gaagagggct acagatggag ttagcacctc aagatatctg tgcattaacg
     6361 agcggacaat ttgcagatat aaaacaataa atcatacgta taaaagaaag aaggaggccg
     6421 ggatgttgtt tgtccagtct ccttctttct tttttataca ttatgctctt tgcccggcat
     6481 tcattcctgc tacatgccct gtagcgaaag ccgctgtgat attaaagccg ccagtataac
     6541 cattgacatc cagtaattct cccgcaaaat acagaccatc aatgatttta ctctccaatg
     6601 ttttaggata aatttccttt agagaaaccc ctccgccagt tacaaatgat ttctcaatag
     6661 gaaaagtttt gttgattgat agcttaaact ctttgcatag ttgaacaaaa tcttgaattt
     6721 gtttttgaga ggcttgttgt cctgtcagat gatccatttc aagccgctca agaaagaaaa
     6781 ctaacaatcg ctcaggcaat ataccatgcc aagcattgac caagttcttt ttggttgctt
     6841 tacttttttc tgtcaatata tgaataagct cttgtttggt ttgtgtaggg aaacaatcca
     6901 gagaaagtgt cactggttct cccgttttct ctaattcttt attgacaaaa cttgaacaac
     6961 gcagtgcagc cggaccagaa ataccgaaat gggtgaaaag taaatccatc gtatgttcag
     7021 tcaatacacg acctttttta tttaaaacgc gtaaagtgat gtcttgtaaa gaaagccctt
     7081 gaagtgtttt ctcctgaata tacggttcat ccgatatcaa gggggactct gtcgcataaa
     7141 gaggcgtcac cgtgtgaccc actcgcttca cgatcttgta cccatctcca gtagaaccag
     7201 tagatggata agtccgtcca ccagttgtta agataatggc aggagcatgg aactcttcga
     7261 attccgtgcg tataccgtaa attttatgtt ctttgtggat caatttctct actcgagtag
     7321 agaatagaag cgtcacatct agttctttca gacgattgag caatgcttcg ataatcgttt
     7381 ttgatttatt tgttacagga aacatccgtc catgatcctc ttcttttaaa tgtacgcctt
     7441 gtgattcaaa gaagttcatg atatcgaaat tattccattg cgcaaacgta ctatataaaa
     7501 actttccatt tccaggaata tgtgcaatca aatcatcaac aggacgattg ttcgtaacat
     7561 tgcaccgacc accacctgtc atcagtagtt tttttcctgc ttttttgttt ttttcgatca
     7621 agagtacgcg tgcgccttgt tcagctgcac tgatcgctgc catcatcccg cttgttccag
     7681 cgccgactac gatcacatca tacagttttt ccattccctc acctcaagga aagtgtagca
     7741 tatctgaaga aagaaaacag aaaaaaatgt tttgagccaa aagagaaaat gagctaaaat
     7801 gagggaagat aagcaaagga gcgtggaacg atgacaaacg caacagaata tgttcaagca
     7861 attcaagaaa agctacatca aaaggatcaa gggcaagaag aatttttaca agcaatcgat
     7921 gaattcatgc ccacggttat gtcttttttg gacactcatc ccgaatatat cgaaaagaat
     7981 atcttagaac ttttaacgga accagaaaga gttattcaat ttcgagtgcc ttggcaagat
     8041 gatgaaggaa actggcgagt aaatagaggg taccgtatcc aatacaactc agctatcggc
     8101 ccttataaag gtggattgcg cttccatcct agtgtcaatc taagtatctt gaaatttttg
     8161 gcttttgaac agattttcaa gaatagttta accggtctgc caatcggtgg aggaaaaggc
     8221 ggaagtgact tcgatcctaa aggaaaatct gataatgaga tcatgcgttt ttgtcaaagt
     8281 ttcatgctgg aattgtcaaa acatatcggc ccatcgattg atgttcctgc gggagatatc
     8341 ggtgtaggag ctagagaaat cggctatttg ttcggcgaat ataaacgatt gaaaaaatat
     8401 gatactggtg tgttgacagg taaacctttg gatttttggg gaagtaagat acgtacggaa
     8461 gctactggtt atggattagt gtattatgtc aaacatctat tgaacgaaga aaaagattca
     8521 tttaatcaaa aaacagtttt tgtttccgga agcgggaatg tagccattta tgcaatcgaa
     8581 aaagtgcaag agctaggcgg gaaagttgtt acatgttcag attctagcgg gtacatctat
     8641 gatccagaag gaatagatgt gaaactattg aaagaagtca aagaaatcaa tagacaacgt
     8701 ttgacggaat acgcaaacca acgtccttct gctatttatc atgctggaga atctgtttgg
     8761 acattgaaag aaaaagcgga tatcgctttg ccatgtgcta ctcaaaatga aatagacgaa
     8821 agcttggccg aaatcttagt agaaaatggg atcaaaatcg tagcagaagg cgctaatatg
     8881 ccatgtacag taaaagctgt ttctgttctt catgaagcag gcgtgtggta ttgtccagga
     8941 aaagctgcta atgctggagg agtagcagta tctgctttag aaatgagtca gaacgtccag
     9001 cgattgactt gggaaagaga gcaagtggat gagcagctgg atcaaatcat ggaaacgatt
     9061 taccatacgt gtcgccaaac agcagccgag tacggcgacg aaaaaaatct gcttcttgga
     9121 gcaaatgttg ctggatttga aaaagttgcc aaagcaatgg cagcagaagg tttagtttga
     9181 aaaatgaaac tttcttggaa atattgtcag cattttgttt atttgcttga aaaataataa
     9241 aaaaaaacgt aaagtattac ctgtagagta aacctataag gaggagaaca aatggcacac
     9301 attcattttg actattcaaa agtagcaccg tttgtcaacg aacaagagtt agggtacatg
     9361 caaagccaag taacagcagc ccacaacgaa ttacgggaag gtactggcgc aggaagcgac
     9421 ttccgcggct ggatcgattt accaacgaac tatgacaaag aagaatttgc acgtatcaaa
     9481 gaagcagcta aaaaaatcca atctgattct gaagtattag ttgttatcgg tatcggtggt
     9541 tcttatttag gcgctcgtgc agcgatcgac ttcttgaatc acacattctt caatttatta
     9601 gatagcaaca caagaaaagc accacaaatc ttctttgccg gaaattcaat cagctcaaca
     9661 tatattgctg acttgatcga agtaatcggt gatcgcgact tttcagtaaa cgttatttca
     9721 aaatctggaa caactactga accagcgatt gcgttccgtg tattcaaaga gcttcttgtt
     9781 aagaaatatg gacaagaaga agcaaacaaa cgtatctacg ctacaacaga taaagcaaaa
     9841 ggtgcagtta aagtagaagc agatgcagaa ggttgggaaa ctttcgtgat tcctgatgac
     9901 atcggcggac gtttctctgt attaactgct gttggtttgt taccaatcgc tgttagtggt
     9961 gcagatatcg atgcattgat gcaaggtgcc gctgatgcaa gcaaagcata ctcaagcgac
    10021 aaactagaag aaaacgaagc ttaccaatac gcagcaatgc gcaacattct ttaccgtaaa
    10081 ggcaaagtaa ctgagttgtt gatcaactat gaaccaggaa tgcaatactt ctcagaatgg
    10141 tggaaacaat tgtatggtga atctgaagga aaagaccaaa aaggcatcta tccttcaagc
    10201 gcaaacttct caactgattt gcactcttta ggacaattca tccaagaagg acgtcgcaat
    10261 atttttgaaa cagttgtgaa ggttgaaaaa cctcgtaaat caatcacaat tcctgaacaa
    10321 gcagaagatt tagacggtct aggctacttg caaggaaaag aagtggactt cgtaaataca
    10381 aaagcatttg aaggaacatt gcttgctcat acagacggtg acgtgcctaa cttgctagtc
    10441 aaaattccag aaatggatgc ttatacattg ggttatacaa tgtacttctt tgaaattgct
    10501 gtaggtattt caggttactt gaatggaatc aacccattcg accaaccagg tgtagaagct
    10561 tacaagaaaa acatgttcgc acttctaggc aaaccaggct ttgaagatct tgctaaagaa
    10621 ttgaacgaac gcctgtaaga taagggtttt tcgctagatt taaataatca gtgatagaaa
    10681 catgttcgct ttaaaatgaa aactggtacc catttaggta ctcaaacata ctgcatcgtt
    10741 tattcagaat aaggggctgt gacaagacgt ttgtcacagt cccttgtttg tatagaaatc
    10801 gattgattgc cttgctcttt agtttgtttt aggactttaa gaacagtgag gctgacttcg
    10861 aaggttaatt ctgttttttt gttttctaga atcagtaagc aaaaaagcac tcacttcgag
    10921 tgcttttttg cgatatattc ttagcttaag ataggcctaa tttttctttt acagatgagg
    10981 gtaccttatc gctggtaatt acttttttct ctatgatccg cttttcgctt gctttcaact
    11041 gcaagatcgt tccttcgggg taagcagaac cattggcaga gtcaccagaa gtaatgacat
    11101 cgaaatctaa ctgtcttttt tcaccgtttt cagcataagc atctacgttg tatttaaaac
    11161 ctcttccaat tacttcccca ttatctgctt tgatatcagt ttctgcaggc attggagctt
    11221 tgactaccgc ataataatct ttacctacat aagtagactg atagtagtcc caacctttcc
    11281 atcctataaa agcaatcaat atgataacga tcaaccctaa aatttttttc atgaaagatc
    11341 tcctttttta ttttta
//
LOCUS       DAFFSV010000097        11240 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFFSV010000097 DAFFSV010000000
VERSION     DAFFSV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.097"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            85..1131
                     /gene="gmd"
                     /locus_tag="LR110_002167"
     CDS             85..1131
                     /gene="gmd"
                     /locus_tag="LR110_002167"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBL4823117.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1211..2224
                     /locus_tag="LR110_002168"
     CDS             1211..2224
                     /locus_tag="LR110_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823118.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            2261..3205
                     /locus_tag="LR110_002169"
     CDS             2261..3205
                     /locus_tag="LR110_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBL4823119.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWKNGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3273..4586
                     /locus_tag="LR110_002170"
     CDS             3273..4586
                     /locus_tag="LR110_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBL4823120.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVISASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4670..5572
                     /locus_tag="LR110_002171"
     CDS             4670..5572
                     /locus_tag="LR110_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBL4823121.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSVSFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            5577..7229
                     /locus_tag="LR110_002172"
     CDS             5577..7229
                     /locus_tag="LR110_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBL4823122.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEKVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKADSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7253..8119
                     /locus_tag="LR110_002173"
     CDS             7253..8119
                     /locus_tag="LR110_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823123.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(8161..8352)
                     /locus_tag="LR110_002174"
     CDS             complement(8161..8352)
                     /locus_tag="LR110_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823124.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCGKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            8382..8921
                     /locus_tag="LR110_002175"
     CDS             8382..8921
                     /locus_tag="LR110_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823125.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGADVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            9049..9402
                     /locus_tag="LR110_002176"
     CDS             9049..9402
                     /locus_tag="LR110_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823126.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            9869..10345
                     /locus_tag="LR110_002177"
                     /pseudo
     CDS             9869..10345
                     /locus_tag="LR110_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            10345..10539
                     /locus_tag="LR110_002178"
     CDS             10345..10539
                     /locus_tag="LR110_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823127.1"
                     /translation="MAETRPKGYPKLKEYTPNRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            10545..10830
                     /locus_tag="LR110_002179"
                     /pseudo
     CDS             10545..10830
                     /locus_tag="LR110_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            10844..11210
                     /locus_tag="LR110_002180"
                     /pseudo
     CDS             10844..11210
                     /locus_tag="LR110_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
BASE COUNT         3155 a         2303 c         2855 g         2927 t
ORIGIN      
        1 aattatcgtg tattcttgaa aagtattaga aattacgagc catttatatg gtcttatcac
       61 ttacatagga aaggataatg tatcatgaaa aaagcgctga ttactggtat tactggtcag
      121 gacggttcct atctggcaga tttcctgcta gagaagggct acgaagttca cggtatcact
      181 cgccgtgctt ctatttccaa taccgctcgc attgaccatt tgatgggcaa gatcaccctt
      241 catgacggtg acctgtccga ctcatcttct ctgattcgta tcatcagcat tgttcagcct
      301 gatgaaatct ataacctcgc tgcacagagc catgttcagg tttcttttga tgttcctgag
      361 tactccggcg atgttgatgc tttgggcgtt atgcgtattc tggaagcttg ccgtattctg
      421 ggcctgacga agaagactaa gatttatcag gcatctactt ctgagctgta tggcaaggtt
      481 gaagaagttc ctcagcgtga aaccactccg tttcatcctt acagccctta tgctgttgca
      541 aagcagtatg gcttctggat cactaaggaa tatcgtgaag cttatggcat gttcgctgtc
      601 aacggtattc tgttcaacca tgaatccgaa cgtcgcggtg agaatttcgt cacccgtaag
      661 atcaccctcg ctgccggccg cattgcagaa ggcctgcagg accatctgga actgggcaac
      721 atggactccc tgcgtgactg gggctatgca aaggactatg ttgagtgcat gtggatgatt
      781 ctccagcatg agactcccga ggacttcgtg atcgctaccg gcgagcagca cactgttcgt
      841 gacttcaccg agaaggcatt cgcagcaaat ggcatgacca ttcgttggga aggcactggc
      901 attgatgaaa agggctacga tgttgaaacc ggcaagatgc tcgtttgtgt aaatccacag
      961 tggttccgtc cgactgacgt tgacaacctg tggggcgacc cgactaaggc aaagaccgtt
     1021 ctcggttgga atccgcagaa gacctcttac gaggaactgg ttaacatcat ggcaaagcat
     1081 gaccgtgaat tggcaaagcg cgaagctgca atgaagaacg cagtcaagta agctgacatt
     1141 gaataaacat atctgtttct atctttccag tcacggcttc ggacatatcg cgcggaatct
     1201 gcccattatt atggcaacac tctgccgatg cgaagcgaag attacactgg tttgcggcgc
     1261 aaatcatctg gattttgcaa agcgcaattt ggagcaggaa ctgcccaaag agctgataga
     1321 ccgcgtgcgc tatcgtccaa taaacacaga tatcggtctg attaaccgtg agggcaccct
     1381 actggtggac acgggtaagc tgaccgagat ctgtgaatat tttttggcaa aggttccttc
     1441 tttggcaaag acagaagccg aatggctcaa gcgagaagct gtcagcgcag tgctttgcga
     1501 tatgccaatc tgggcaatgg atgcctgcga tatggcagga gtacctttcc tgtatgtagg
     1561 aaacttcact tggacagagc tgtatcgcga atttttaccc gaacatattt ggaaagaata
     1621 tgccaagcgt tacggtaaaa ttcagcacac catgctttat gcgctaaata acgaagaaat
     1681 gttggaattt ctccaaaaca gcgaaaaaac accgacttcc gttgtggcgc gtccgattca
     1741 taacgacgaa gtgcaacgca ttaaatcaca gcatgaaaaa ccgcctgtat ttgtcgcact
     1801 cggtatgtcc gcgcggttca aacagccaat tgatgtcagt cacgcgccgt atgatttcat
     1861 tacaacgcta ggtgttccgc ttgtgggaga aaacgtaacc gttatttcta acagcacccc
     1921 caatacacag gactatatag cggcggcaga ttacgttatt acaaaggctg gatggggaac
     1981 tgttgcggag tgtattctcg cacagcgtcc gatggcactg ttcgcccgcg atacagtttt
     2041 ggaagataga acaactattt gtaaattgca aaaactgggt ctggcaattt ccgtcacacc
     2101 ggaaaacctc caccgcgtag agcgggtgat tgaggacctc aaacagctag acacctcaaa
     2161 ttttgataca ttttatgatg ctgcgagcga aatcgcagac aaattgattt ctctcatgca
     2221 atgagagatg ctctaaattt agaaaggagc aagcattatt atgatggaaa aagacgcaaa
     2281 aatctacgta gccggtcatc gtggcatggc tggttccgca atcgtgcgcg agctgaaccg
     2341 tcaggactac aacaatatta tcacccgcac ccataaggag cttgacctgt gccgtcagga
     2401 cgctgttgaa gcatttttcg cacaggaaaa gccggattat gtatttctcg ctgctgcaaa
     2461 ggttggcggt attatcgcaa accagaacgc tctggcagac ttcatgtatg aaaacatgat
     2521 tctggaaatg aacgtcatca actccgcatg gaagaatggc tgtaagaagt tacagttcct
     2581 cggttcttcc tgtatctatc cgcgtatggc accgcagccg atgccggaaa gctgtctgct
     2641 gacaagtgag ctggaaaaga ccaacgaggc ttatgcactg gcaaagattt ctggcctgaa
     2701 gtactgtgag tttctaaaca agcagtacgg tactgactat atctctgtta tgcctactaa
     2761 cctgtatgga cctaacgaca actatcatcc tactcacagt catgtgctcc ccgctttgat
     2821 tcgtcgcttc catgaggcta aggaagcagg tctgcctact gttacctgct ggggtgatgg
     2881 ttctcctctg cgcgaattcc tatatgttga cgatctggca aacctgtgtg tgttcctgat
     2941 gaataactat agcggtgacg agactgttaa tgcaggtact ggcaaggaac tgtctatcaa
     3001 ggaactgacc gagatggtag caaaggttat tggttacgaa ggcgagattc tgtgggatac
     3061 cagcaagccg aacggtacac ctcgtaagct gctggatgtt tccaaggcaa ccaagctggg
     3121 atggacatat aagactgagt tggaagatgg catccgtctg tcctacgagg acttcctgaa
     3181 taacccgatg cgtgctgaac gctaatttac cgaggttgct gtgatttttg aggattgcag
     3241 cagcctcttt cttataaaaa ggagagatca ttatgaatat tgttttactg tctggcggtt
     3301 ctggtaagcg cctttggccg ctgtctaatg acatccgttc caagcagttc attaagattt
     3361 tcaaaactga agatggatat gaatccatgg ttcagcgtgt ctaccgccag attaagaagg
     3421 tagataacga cgcaactgtt acgattgcaa cctctaagac acaggtttct gctatccata
     3481 accaacttgg tgaggatgtg ggtgtatctg tagagccttg tcgccgcgat accttcccgg
     3541 caatcgcatt ggctaccgct tatctggtag atgtgatggg cattgatcca gaagagtccg
     3601 tagttgtttg cccggttgac ccttacgtag aagatgacta ttttgaggcc ctgaaatctc
     3661 tgtccgatca ggctgacaaa ggcgaagcta atctggtgct catgggtatt gaaccgactt
     3721 atccttccga gaagtacggc tacatcattc ctgagagcgc agagcagact gctttcgtaa
     3781 ataccttcaa ggaaaaacct tctgaggctg tggcaaagga atatatcgca cagggtgcac
     3841 tgtggaacgg tggtgtgttt gtctacaagc tgaagtatgt catggagcgt gcacatcagc
     3901 tgattgactt tgtagattat caggatctgt tcaacaaata cgatactctg actaagattt
     3961 ccttcgacta tgccgttgtt gagcatgagg acaagattca ggtacagcgt tttgctggta
     4021 tgtggaagga tctggggact tggaatactc tgaccgaagg tatggaggaa tccattatcg
     4081 gtaaaggtgt tatgaatgag aattgcgatg gcgtacacat cctgaacgaa ctggatgttc
     4141 ctgtgttggc tatgggtctg catgatgtgg tcatctctgc atctcgggaa ggtattctgg
     4201 tttccgataa ggaacagtcc agctatatta agcctttcgt tgacaagttc gagcagcaga
     4261 tcatgttcgc agagaagtct tggggtagtt ataaggttat ggatgtagaa gcagagagca
     4321 tgaccattaa ggttactctg aatgctagtc actccatgaa ttatcacagc cacaagaacc
     4381 gtgacgaggt gtgggtggtt ctggccggta ctggtaagac catcgtggat ggatatgagc
     4441 aggttgttaa ggctggcgat gtgatcacca tgagtgctgg ctgtcggcat accattatgg
     4501 cagatactga gctgaagctg attgaagtac agcttggcaa ggatatcaat gttaaagata
     4561 aacagaaatt tgagttagag tattaaaaaa cggaggaggg ctcgttatac agaaaccgtt
     4621 tttattgaag ccttccgcaa aggattatct gtggggggga accgcctgaa tgatgaattt
     4681 gcaaaaggaa ttgagatgtc tcctcttgcg gaaacttgga atgctctacg cacccggatg
     4741 gaccgagctt tgttgtgtct ggtcagagta tcaggggatg accttgacgg aggtgttgca
     4801 agaacaccca gagtttctgg gaacgcatcc gcagaccaaa ggagaattgc cgatcctgat
     4861 caaattcatt gatgcgaaaa aggatttgtc tgttcaggta catcccacag atgaatatgc
     4921 aattgaaaaa gaaaacgggc agcttggaaa gacagagatg tggtatgtac tggatgccag
     4981 cttaatctat gggctgcaga aaataacaga agaagctgtg atccgtgaag cgattgctca
     5041 gggaactgtt gagcgttatc ttcagaaggt tccggtcaaa aagaatgatg tgttctttat
     5101 tgaggcggga acgattcatg cgattggtgc gggtgcacta attgcagaaa ttcaggaaaa
     5161 ttccaatctg acttatcgtc tctacgatta tgaccgtgtt gacaaaaatg gtaaaaagag
     5221 agaacttcat gtagacaaag cgctagaagt agcgaacttg aaagggttgt cagaaccgaa
     5281 acagccgctt cgtgttctaa aatatcgtgc tggtgtagct gcggaattgc tttgcagatg
     5341 caagcatttc gaggtctctc gtatgattgt caacactgag agaagacaga cggtacacta
     5401 tcgtgcggat tcagtcagct tccgtgtgtt gctgtgtgtc aatggctgtg gtaccgttcg
     5461 atttgataat gaatcgttgg acatctataa gggggactgc ctttttgtgc cggccgattc
     5521 tactgaaatc agcattcacg ggtcgattca gtttctggat gtgagaggat aataatatga
     5581 attataaagt agaatatgag cgttggctga atcatgccaa tgcagatatt atagaagaac
     5641 tgcgttccta ttctgagaag gacattgagg actctttcta taaaaatttg tctttcggta
     5701 ctggtggtct gcgtggtacc atcggtgcag gcactaatcg catgaacgta cacaccgtag
     5761 gtaaggcaag ccaaggtctg gcagactatc tgaataagaa ctatgaagcc gcttccgtgg
     5821 tggttggcta tgattcccgt atcaaatctg atgtgtttgc aaagactgcc gcagacatct
     5881 ttgctgcaaa tggtattcag gtgcacttgt ggccagaact gcttcctgtt cctacagtat
     5941 cttttgcgac tcgttatctg aaggcagatg ccggtgttat gattacggca tctcataacc
     6001 cttctaaata taacggctac aaggtatacg gtgcagacgg ctgtcagatt actaccgagg
     6061 ctgctactgc aattctggca gaaatcgaaa agctggacat ctttgctgat gtaaagatgg
     6121 atggcgatgc ttctaagatt gcatccattc agtcggaagt tctgactgct ttcatcgaag
     6181 aagtgaaggg gcagtccgtc ctgtttggcg aaaaagtgga caagaatgtt gctatcgtgt
     6241 attctcctct gaatggtacc ggtctggtgc ctgttacccg caccctggca gagatgggat
     6301 acagcaatgt gaccgttgtg gaagaacagc gtctgccgga cggtaatttc ccgacttgtc
     6361 cttatccgaa ccctgaaatt aaggaagcta tgtctctggg tatggagtat gccaagaagt
     6421 acaatgcgga tctgctgttg gccactgacc ctgactgcga ccgtgttggt atcgcagtta
     6481 agagcgcaca tggtgactat gttctgttgt ctggtaatga gactggtctg ctacttctgg
     6541 actacatctg ttctcagaga attaagcatg gaaaaatgcc tgctgaccct gtgatggtca
     6601 agaccatcgt caccatggat ttgggtgaac gtatcgcatc caactatggt gttcgtacca
     6661 tcgatgtgct gaccggcttt aagtttatcg gtgagcagat tggtatgttg gaaaaggctg
     6721 gtaaggctga cagctatatc ttcggctttg aggaatctta cggctatctg tccggtagct
     6781 atgtccgtga caaggacggt gttaacgctg cttatctaat ctgtgaaatg ttctcttact
     6841 acaagactca gggtatcagc ctgctggata agctgaatga gatttataca aagtatggct
     6901 attgtttgaa caccctgcat tccttcgagt ttgacggtgc ggcaggtttt accaagatgc
     6961 aggaaattat ggttgaattc cacaagggtc tcgatataat tggtggtaag aaggttattg
     7021 aaactttaga ctacagtaag ggcctgaatg gtctgccgaa gtctgatgtt ttgaagttta
     7081 tgctggaaga taactgctct gttgtggttc gtccttctgg tactgagccg aagctgaaga
     7141 cctatatcag cgtcagcgca gagaacaagg aagccgccga agctatggaa gctattatgg
     7201 ctgctgaact ggcggaaatg ttcgagtaag aaaagaaagg agaaatcttg taatggagga
     7261 actgttttct aacgctttga aatcggtgaa gtcaaatcgt gtgctgtaca cggcatcgtt
     7321 ctttgcaaga tcctctcttt tgtatttgca ggaaattgga cagctgactg ctctgaaggc
     7381 acatacatct aggcgtacag gattgtcatc ttacctttgt ttcctggtag aagttggttc
     7441 aggcacattg gagtataagg ggcagaaata tacgcttcaa gtaggagata tggtgtttat
     7501 tgactgtttg aagccgtatg tccatgagtc atcagaagat gatttgtgga ctttgcggtg
     7561 gtgccatttt aatggcattg gtatgacgga agtctacaaa aaataccttg aacgaggtgg
     7621 tcaaccggtc tttaaaacat caaaaacaga taagtatgct gacttgctga ggagccttca
     7681 tatggtggca gagggacagg agtatgtccg ggatatgaaa atcaatgttc tactcagtga
     7741 actgctgatg catttgatgg aagattcttg gaatcctgac aaagtgccga atttctctac
     7801 aaaaaaaatg gaattgcaga acataaaaca atatctggat gaccattaca cggagaagat
     7861 tacgctggat cagttgagtg cagagttttt tatcaataaa cagtacttag ctaagatttt
     7921 tcgtgaaact tacggtacaa ctgttgtcgg ttatctgaat cagaaacgaa tcacacaggc
     7981 caagcacttg ctacgcttta caaatctaag tatggaagag attgcaacaa aaattggcat
     8041 aacagatgca aactattttt ccagaacttt tttcaaggtg gaaggtgttc gtcccagcga
     8101 attccgcaag atgtggtaat aaaaagccgg cggtgttgag ccgccggtca gtacctgtcg
     8161 ttaacccgca ttcaaagctt taatctcagc tttgatttca gcacgaacac gatagtaatc
     8221 ttcagtttcg atttcggtat ccagccgagt tgctgtcttt ccgcagtcag gacaacgctc
     8281 tggctgttcg tcggcaggga agctgtagtg gcaggaatca caggtgtaaa tgtgatcgtc
     8341 acgtataatc atcacctacc aatccagtgc ttagattttt catgcaatta ctattatatc
     8401 gaattggtga ttccacaaaa atccgcttct tgataaatgt acgattcaga gtgatatata
     8461 gtactgtact gccaaaggga ataccacaac ggcagaaatg gatgattcac atggaagaaa
     8521 ttatgcagat gattttaggg aacgcaaaat tccagctcgg acaggtggtt tcgacccctg
     8581 ctgtattgga aactttcaac cccctgcaga ccgcaaacgg cattcccgaa gccttgagca
     8641 acctttggtt tagacaggaa attagtattg cggagatcga ggacgcagtt ctggatgcca
     8701 agaacggcaa ggagctagca aacagcctca accgcatcaa gcttttccgc aagttcaccc
     8761 tcgaccgaga gctggacggc atggtcagac tgaagggcgc ggacgtctgg aagaacatca
     8821 gctacctcaa ggtctacacc gccacgccgc ctaagaaacc gaccccccca aggttccagt
     8881 cgaacggctg gagccttttc ttctctcata aatcggcata atgtatacaa atacagctcc
     8941 aaatgattgt gtagtatatg cctgtggatt gacttgatat agtcgagaga ctatggcaat
     9001 atacacacaa ccaaagggga aaatcccaaa aacaaggagg aaacgctaat ggcaatgagc
     9061 gaaaaggcaa agaaatattt gaaggagatc aagggtgcaa agaccatccc ggagctgaag
     9121 gatgtggaga tcgcaatcaa gcgagacggt atccttgctt gggcagagtt caccaagttg
     9181 aacgaggcgg tggaagagaa gaaggtcgcc cttcgcaaga aaaagcagga gaccagcctt
     9241 caggagatcc ttttctgggc ctacaaaaag gaatcagaca agctgctgaa gatgatggac
     9301 gagggagcag acaaggatgc catccagatg caggtccagc gttacgacag catcggccag
     9361 atcattgcag aggcagacct cgaagatgag tatgaggttt gagaatacag cctgaaccac
     9421 taagaaccaa ataccgaaag ggacttctga aacagacaga ggtccctttc ttttgctcac
     9481 tttggcggtc aattatgaac caaaaaccag tcgaatgatt gtgtacatta tggctttcaa
     9541 acgacttgat aataatcact tttagagtga tatatgtact acaaaaaaca aaagactgga
     9601 gaaaacaaca gatgagcgaa tacagaatca tttatatcag cgacgaggac ggcggcgagg
     9661 tggaaggatg tggctacacg gctacgatgg ttatgatgcg gtcagaaact tcttggaatt
     9721 tgcctgcggc atcttgaaaa tcctgcgggt gcgattcatc agaagagtgg actgaaatac
     9781 aagttagcag atagagaaga gagtcaggtt tcgacttggc tctgttctct gtctactggc
     9841 agaaatacat tgaagggagg tgacgactgt ggcaaccaga ggaagaaaat caaccccgac
     9901 agccatgaag aagctggagg gaaatccggg caatcgccca ctcaatgcaa aagagtcaac
     9961 gccggaaaag aaggcaccga cctgtccgaa gtggctggaa ccagaggcaa agaaggaatg
    10021 gaagcgtctg gcaaagcaga tggaggccat cggaatcttg acagatgtgg atatggctgc
    10081 ctttgccggt tagtgccaag cctacgcccg ctggaaggaa gccgaggaat tcattaccca
    10141 gcacggtatc atcgtgaaaa ccctatccgg ctattggcag caggtaccac aggtgtttat
    10201 tgcccagacc tatctgaaaa ttatgaaccg attttccgag cagttcggtc tgacgccttc
    10261 gtccagaagc cgtatcattg ccggtgatgg cggtggtgtc ggagcagaag atgcgatgga
    10321 ggctctgttg ggaggtggta gctgatggca gaaaccagac caaagggcta tcccaaatta
    10381 aaggaataca caccaaaccg attcatgctt tcggaatgtc actacgacaa agccagagcg
    10441 gatcgggcgg tgaactttat cggacagctc cgacacacca aaggcaaatg ggcaggcaac
    10501 cgcttctggc tcttgccttg gcaggaacag atcatctagt gtaatctcgc ttaactgtat
    10561 taacggacgt agcgacttta ttaagagtgt tcatggtagg agcattagtt gcaacaatga
    10621 ttgatattaa atttttatgg taccaacgat tggcgatgtg acattgaata tgcaaaacta
    10681 tatcgatatg atattatcta aaatcttact cgctgggatc gtcgaggtgt ttattggttg
    10741 ttaggaaaga aaaatatgac ccaactcgag cgatttttat cgtattactt gtgtgtggct
    10801 ttatctgcat tgtgtgtgat ttcgaaataa gggagagcca aaaatgagta gaaaattgat
    10861 attgattagt cacggaaaat tttgtgagga attgaaaaaa agtacaaaaa tgattatggg
    10921 acctcaagag aatatctaca cgttaccttt attaccagaa gatggcactg aaacatacag
    10981 acaaaagctc ttactgtcat agattcgtta aatgattttg tggtactatg cgatctttta
    11041 ggcggtacac ctactaatat catttctaaa ctgataatgg aaggacaaca gattaaactt
    11101 tatgtcggat taatcttcct atggtcattg aatttatcaa ctcgcaagtg attggttcag
    11161 agcccgagtt tgttcgtgca ggatgtgaaa gcattgtatc tgtcaactaa ttaattagtg
    11221 gaatagtaga agatgaagag
//
LOCUS       DAFFSV010000098        11210 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFFSV010000098 DAFFSV010000000
VERSION     DAFFSV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.098"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..291
                     /locus_tag="LR110_002181"
     CDS             <1..291
                     /locus_tag="LR110_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4823128.1"
                     /translation="RLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSIQNE
                     LLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            364..1725
                     /locus_tag="LR110_002182"
     CDS             364..1725
                     /locus_tag="LR110_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL4823129.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1747..2007
                     /locus_tag="LR110_002183"
     CDS             1747..2007
                     /locus_tag="LR110_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823130.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2021..2773
                     /locus_tag="LR110_002184"
     CDS             2021..2773
                     /locus_tag="LR110_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBL4823131.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2867..3472)
                     /locus_tag="LR110_002185"
     CDS             complement(2867..3472)
                     /locus_tag="LR110_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL4823132.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3525..4499)
                     /locus_tag="LR110_002186"
     CDS             complement(3525..4499)
                     /locus_tag="LR110_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBL4823133.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4877..5596)
                     /locus_tag="LR110_002187"
     CDS             complement(4877..5596)
                     /locus_tag="LR110_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4823134.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5723..6994)
                     /gene="celB"
                     /locus_tag="LR110_002188"
     CDS             complement(5723..6994)
                     /gene="celB"
                     /locus_tag="LR110_002188"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL4823135.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7147..8544
                     /locus_tag="LR110_002189"
     CDS             7147..8544
                     /locus_tag="LR110_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL4823136.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8625..9065
                     /locus_tag="LR110_002190"
     CDS             8625..9065
                     /locus_tag="LR110_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL4823137.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9213..9539
                     /locus_tag="LR110_002191"
     CDS             9213..9539
                     /locus_tag="LR110_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL4823138.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9540..10289
                     /locus_tag="LR110_002192"
     CDS             9540..10289
                     /locus_tag="LR110_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823139.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10369..10824)
                     /locus_tag="LR110_002193"
     CDS             complement(10369..10824)
                     /locus_tag="LR110_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL4823140.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3548 a         1960 c         2301 g         3401 t
ORIGIN      
        1 cggttaattg tcgcatgtgg aagtggagta gcaacgagtc agacaatcgc tagtaagatc
       61 caaagtatgt tagaagatga cggcatcgca tttcctgtag aagcagttga ttacaaatcg
      121 attcaaaatg agcttctaac tgctggaatc tatgtgtatg tcgcacaacc tgatgaggaa
      181 gtacttgaac aagcaaaaga tttgggtatt gaagtatttc caggaattcc atttttaact
      241 ggaatgggtg tagaacctat ttatgacagt atcaaagagt tagtacagta aaggaggatt
      301 gttggataaa aaagttggtt catagtttta tttactatat ttagaaagaa agaaggttta
      361 tttatggaag ttttccaaac cattatcaat tatgttttga acttaggatc agccattttt
      421 gttcccctga ttattttgat acttggattg ttggcaggga tgaaattaaa aaaagcattc
      481 atgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat tggctatatg
      541 tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat cagtttgcct
      601 gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag ttatgctttt
      661 atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa ttggacgaag
      721 acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta tttagtttat
      781 tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt tatattggaa
      841 ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat tccattagta
      901 acagtgactc attttatgac atctgccgct gtcttgttgc tgcctttcaa tatgatcatg
      961 gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa gaaaattggg
     1021 atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg ctttgctgct
     1081 gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc attaagtttg
     1141 tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga cgctatgtca
     1201 gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga ctggccgata
     1261 ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt gtttattggt
     1321 tatgccatta ttttgccagg aaaccaagta ttgccattag cagggattat caactattcg
     1381 attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct gatcttggga
     1441 atcatcacta tgcctattta tttgtacggg gccacttacc ttgcgccaat actcagtgat
     1501 ttagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat cacatggtca
     1561 tccatagaag ggccggagtt cagggtttta tttgcggagg catttaatag gaatattctc
     1621 gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa atatatgata
     1681 aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg aaatggtggg
     1741 aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct ttgtggtaca
     1801 cttctgctat ttctatatgg actctttact cagaatatca tcatcaatct attggtcatt
     1861 tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga gtacgatgaa
     1921 aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa agaaatattg
     1981 agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg gacgtatgtt
     2041 atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg aaaggaaaaa
     2101 cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag ggaaagacta
     2161 catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt ctcctgcaca
     2221 ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc atttaactcg
     2281 tgaaatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag ctgtcctaca
     2341 tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc ctaatttgac
     2401 agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta attgctctga
     2461 agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat taccgcatat
     2521 gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag gaatcgaagc
     2581 tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa ttgcctacaa
     2641 gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag gtgagaaaat
     2701 cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg ttattgcagc
     2761 tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc tgatccagat
     2821 cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat ttagttaaga
     2881 ggtggtctgt ccattccctc gcttgcttga tttcgtccat cgtcaactga tgacctgtta
     2941 cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc aattctttaa
     3001 aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg gttttatcct
     3061 ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac aaccctttat
     3121 ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca tttgaatagc
     3181 cgacaagcag ccagtcactt aacggtatca gttctttttc gctgatttct ttgatttttt
     3241 catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc aatccattac
     3301 gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa agcaatgtac
     3361 tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca cctgtgccat
     3421 gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc attttgttcc
     3481 ctcttttctg tttctataga tgcaaatgaa gtagcatgaa gagattattc acgtttttta
     3541 tttgcatcac ttgtatcgaa aggacggaca taagcttcaa tggcttcacg acgttcttct
     3601 aagaatggag gaagagatag tttttctcct gctgattctg ctggttcatc ttcaaggaat
     3661 ccaggaccat cagttgctaa ttcaaacagt acgtgatgag cggctaggaa gtactcagat
     3721 tggaaataga aacgatcgac gaaaccagaa tttggcaatt caatcttatt gattacttga
     3781 atccagtatt ccaatgcttc tttgtctgct acacgcaatg ctaagtgatg gatatttcca
     3841 aatccttcga ttgcttttgg caagtctgtc cgatgttcaa taatgatgct tgcaccattt
     3901 cccccttcac cgatttcaaa taagtagaaa tctcctgaat ggtctgtttc tttgaatcct
     3961 aagatttttt caagaatcaa gcggatatgc tctaattgac tgacacggac aaagacaggt
     4021 ccaagtcctg tgatcgcaaa ctctgtcggt acattgctgt ttttccaagg aatgccagct
     4081 gctacacctt gattgttttc gtcagagatc aattgataat actgattatc gaaatcttca
     4141 aattcgatat attttttgcc gaaacgttca ttgattgctg aatgagtaac gtcatatttt
     4201 tcaaaacgtt ccaaccaaaa gtctaatgct gcgtcacttg gcacacggaa agaagcacga
     4261 gagatcgtat ctgttccttt ttttcctttt ggctgatttg gaaaatcaaa gaaagtcata
     4321 tctgttcctg gcgcaccagt atcgtctgta aagtaaagat ggtaagtttc gatgtcatct
     4381 tggttaactg tttttttgat taagcgcatt cctaaaatat ctgtaaaaaa gcggtagatt
     4441 tttgtagcgc tagatgtcat tgctgtgacg tggtgaattc ctctgatttg tgtatccatg
     4501 ataaatttcc tcctgttaac ttacaaaaag taagtagttg atttagacat agtatagagg
     4561 gtgcatctct attttgcaat taatttgtct tctaaagtat caggaaggaa tcaagaatgg
     4621 aaaagaggag ggacggatca ataaaataaa aagacttacg actagctgta tcgatttcaa
     4681 aaaaactagc tttaaaaagc taactttaga atcattacgg gtggtcataa gtctttttat
     4741 gctgagttat tttttcattt ttgagctaat gcttaggata gaatagaatg ttgatttcac
     4801 gatctcttct tgttacttga aactgcttac cactgtcacg atctcttggt aaacggcttt
     4861 ttcacaacct ctttggttaa gttgaacgca catccagcac agaatagttc cgaacatcaa
     4921 atcggtttcg gctggaagaa tattcaatcg gctgtccatc tttgagatag acgacttgct
     4981 gaacttccag tacaggatcg ttttctgcac agttcaaata ttgctgatca tattcatcac
     5041 ttttatctgc agaaatagca cgataagccc ctgcaaaatg gacattcaat tcttgatgaa
     5101 gatgatcata gatagaggct aacaaatgtt ctttttttaa acccggcaca agatggactg
     5161 gcataaaggt atgctctaaa acatatggct cgccatctaa gatccttaac cggtgaatat
     5221 tgtagacagg ttgctcggca tcgatcatca atcgttcttg gatatattga ttagggaatt
     5281 caacatcaaa atgaatgatt tgactcttca aagaacgatg tctgcgtttc atttggagac
     5341 tcaaaccttc atactcgtta gcgggcgatg tgtctttttg cataaatgga tgattcaaga
     5401 ttttcgttcc tgatcctcgc tgcgaataga caagtccttc cattgctaag atattgatgg
     5461 cttttttgat tgtcatccgg ctcgcaccaa attcttttac caagtcgact tgattaggca
     5521 aaaaactatc gagaggatac tcttttgaac ggatacgttc tcttaaaata tttgcgattt
     5581 cttcgtattt tggcacgtta ttcacctctt ctagagtata acgaaagatt atgcttccga
     5641 caactcgaag tagcatctgg aacattgttt attcgaaaaa agaggtcatg acaagttttt
     5701 gtcacaaccc cttaatgatc tattaagcaa tatctaaatc tggttgttta tctagtactt
     5761 tcaggaatgg gtaccagata aaggcaacga tgacaaagtc gatgacttgc agcataccac
     5821 caagccatga atttgttgct aagacaccgc ttgcaatgat tggcacagtc catggaacag
     5881 agacgccagt tggtgcaggg acaagtccag cagccattac caaataattg aacgtcgtta
     5941 cgattagagg tgccacaacc caaggaataa ggattgttgc attcaatacg atcggtagac
     6001 cgaagatcgc tggttcattt acattgaaaa taccaggtgc taaagctaat cgtccaacat
     6061 cacgatactg tttcttttta agaacaaagg ccatcaataa aacgacagct aaagtcattc
     6121 cagagccacc aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg atgtgtggta
     6181 attcttttcc atgttgataa gcttctaagt tatcaagcat cagtgtattc caaatcgggt
     6241 ccattacaga gttgacgatg atttgtccgt gaagtccgaa gaaccataag aattggacaa
     6301 agaacaaagc aatcaatgtt gcaggtaaac tacttcccaa accagttaat ggtttttgga
     6361 tcacttcata aacgacgtca tgtaagttag cattgaaaat accagtcatc acagcattga
     6421 ttactaagaa aatagttagt gtcactacag cagggatcaa agcggcaaat gattttgtta
     6481 ctgcaggcgg aactccatca ggcattttga tctgccagcc gcgtttcgtg attctgcagt
     6541 agatttctcc agccaagaaa gcagcgatca ttccaatgaa cattcctttt gcgccaagac
     6601 gatcaagtga aagcacacca gtcacatctt caccgccagc ggttttcatt gcaaaaggcg
     6661 taagaatcaa gaagctggca aaagacacgg caccaccgaa aataccttca acgtcataag
     6721 attttgataa ataatagccg ataccaaaag taacaaaaac cgacatgatg ctcattgttg
     6781 cattttgccc attaccgaat aaattcgata aagttccctt tgttgcgtca ctgaaaaaag
     6841 gcaagttatt gataacgaca acgatcgaac cgaacatcgt taaagggaag gaaagcatga
     6901 aagcatcccg cagcactgta aggtaacgat tttgccctaa taaattagct aagggcatga
     6961 ttttctcaga aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa tttatttgca
     7021 atcgattaca tacaaagtat aacgccaaaa attttaaatg tctagtcatt ttcataaaaa
     7081 gtattgattt attttttagg gttcgttatg attacggtat caagaaaaaa gagaggaagt
     7141 aaaaaaatga aaacctacac attcccagaa aatttttggt ggggttctgc tgctagcgga
     7201 ccgcaaacag aaggaagagt aagtgatgat ggtaaaggag aaaatatttg ggatcattgg
     7261 tatgaattag agcctgaaaa attcttcgat caagtaggac ctgaaaaaac ttcgcaagtt
     7321 tacacaaaat acaaagaaga tgttcagtta atgaaagaaa ctggccataa cacatttcgt
     7381 acatctatcc aatggagtca tttgattcca gaagggacag gtgaaatcaa tcaaaaagct
     7441 gtttcttttt atcgagatta ttttactgaa ttgctggata atggtattga accttttgtg
     7501 aacctttacc atttcgatat gccactacca ctgcaagaaa aaggcggatg gttatcaaga
     7561 gaaacggtcg acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt tggagatctg
     7621 gtcaaaaaat ggtttactca taatgaacca atcgtacctg tagaaggagg ctacttgtat
     7681 caattccact atccaaatca tatcaatatg aaagaagcgg tccaagtagg ctatcatgag
     7741 aatctagcaa gcgcaaaggt aatcaaagcc taccatgaag gcggacatga tggtgagatc
     7801 ggaatcatct tgaatctgac accaagctat ccgagagatg aaacgaaccc agaagatgta
     7861 aaagcggcga agatcgctga cgcattcttc aaccgatcat tccttgaccc tgcagtgaaa
     7921 ggagagtttc cacaagagtt gatcgaaatt gtcaaagaat tggctataat gccagagatc
     7981 catgaaggag acctagaact catccgtgaa aacacgattg acctgctagg aatcaattac
     8041 tatcagcctc gtcgaatcaa agcaaaagaa actccaatca atcacgaaca tagccccatg
     8101 ccagatgatt attttgataa ttacgacatg cccaataaaa aaatgaatcc atatcgtgga
     8161 tgggaaattt acgaaaaagg aatctatgat attttaacga atacaagaga aaactatggt
     8221 aatatcaaat gctatatttc tgaaaatggg atgggggtcg aaggagaaga acgatttgtt
     8281 aacagcgaag gaattattga agatgattac cggatcgagt ttgttacgga tcatttgaaa
     8341 tacgtccatc aagcaatcca agaaggaacc aattgtgtgg gttaccacat gtggacatgt
     8401 atggacaact ggtcttggac aaatgcttat aagaatcgct atggctttat cgctgttgat
     8461 ttggcacaag acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa aacggtctct
     8521 gaaaaaaacg gatttgatgc ctgatatttt ttgataaaac gttcacaaaa tgtttgttct
     8581 aacactgtaa tcgtgctaca ctaacagtga aggaggcgtt tacaatggta gatacttatc
     8641 gtaatatttt agtagcaatt gatggttctg aaaaggcaga aaaagcattt cttgaggcaa
     8701 tcacgattgc caaacgaaat caggcaacgt tgcacatcct gtatgtcaat gaagtaacag
     8761 gcaattactt tggtgatttt gcatttgtta caacaagcct tcaggaagaa ttggatgagg
     8821 ttgcggaaaa ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc ctgacagata
     8881 tcgagacata tgttctttac ggttatccaa agacactgat tgctaatttc catgaatcga
     8941 aagaaccaat cgatctgatt gtcatggggg cgacaggtct gaatgcggta gaacgtgcac
     9001 tagtaggttc cactacttcc tatgtagtca accacgcatc atgtaacgtc atggttgtaa
     9061 aataaataaa ttaagggaac agaaagcaaa aaagcataac gcttcttttg ctttctgttc
     9121 cttttatttt tttgtaaaaa attactttac ctgatgaagt aatcatgcta ttctaattac
     9181 aacgacagat ggagtaatat ggaggaaaga aaatgatttc aagtgatgga atacgcggat
     9241 acaatgatgc gattatttta tcgatcctta acaaaaacga ttcttatgga tatgaaattt
     9301 ctaaaacgat tcgtgagagg acaaatgatt cctatgtgat caaagagaca acactttact
     9361 ccgtttttgc ccgtttagaa aagaatcaac tgattgtttc gtatcctgga gaagtgactc
     9421 atggaaaaaa gcggacatat tataaaatca ctgaaatagg gcgtaatctt ttagaagaaa
     9481 aaaagcaaga gtggctgacg acgaaagacg tagtcgatca atttttggag ggaaattaaa
     9541 tggatacaat ccgtaattat ttagacagtc tttttatcgg agtacctcaa agtaccgaga
     9601 tagataagct gaaaactgat ttgttggcaa atatggaaga ccattatcat gaattgatgg
     9661 gagaaggaaa aaatgaacag gaggccatcg gtacagtaat ttcgacattt ggttcaatcg
     9721 atgaattatt agaagaacta gatgtcgaaa aaaaacatca agctgacgag acggaaacaa
     9781 acacagcttc tatttattta agcgaagcag aaaactactg gaaagaatac agagcggcaa
     9841 gtctccaagt ggcatcaggt gttctattca ttagcttatc ttttgcaagt tttcttttct
     9901 tttgttcagc aggatatgtg ttcatgggta tttcttgtct gattttcggt atagcattag
     9961 ctgtcggttt ttttattgct tctggtatga aaatcacacg tttaaatcac tttttgcacc
    10021 atcggaaaat acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa gagtatcagc
    10081 gaagttttgg cttctcactg attgcaggaa ttggattatg tattttttca cttttcccac
    10141 ttttggcttc tttaatgtgg tatatggatg gtagtatcgg tgcctctata ttttttgtta
    10201 cggtaggaac gggtgtcttc ttgatcattt atggcagtct agtccgtcat tcttatcgtc
    10261 aattcactca aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa tattctattt
    10321 atccggcgta gaaaaaaaga ctgtgacacg cttttgtcac agtctttttt atatataata
    10381 atttaaaaat tcatcatcta aatccgtaga attttctaga ttgcttaccc atgtaaaatc
    10441 gttatagaca gatttcagtt gacttctgcg ccattgtaca gggataaaaa gacgcatgct
    10501 atcttcattg aatccgacta ataatttctt tccgtcagta atgatgggac gttgtaacaa
    10561 agtaaaatgc gagacgatca gatcgacaag ctctgaggtc gtacattggt cgatttggtc
    10621 tttcaaattt ttataatcaa tcgagcgttt agctaataga tcgtcaaatc cgttttctgt
    10681 taatgacaat aaataaaata tctctttcct tgttaaaggc tgtttaggtg tgaaaattcg
    10741 ttctctgtaa ggcatttgat ggtctttcag ccagtttgcc atcagatggt aactatgtgt
    10801 gcgagaagaa tagatcgtaa gcataaaaaa actccttaaa tagtccatta atttgttaga
    10861 tctgttgaga taaataatag gtaaggagct gtgcataagc attgatcaaa acaacccaaa
    10921 catcattaga aataactttc taaagtctta aacttgcgac gagaaacaac tagactaata
    10981 gaaaaaatgt cttagccatt tggcttcctg tttgtttttt tgcttatgct gcagctcctc
    11041 gggaaagggt tctaacaatt tgaagaaggt tgatgattga tctagtttaa attgtgtgaa
    11101 atttgaaatc tggtgttttt actagatgaa tcacctaaca actatgatta taacttatag
    11161 aacttctgtt ttgcattcgg ttttgttata gttgttataa agttttattt
//
LOCUS       DAFFSV010000099        11063 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFFSV010000099 DAFFSV010000000
VERSION     DAFFSV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.099"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(87..2096)
                     /gene="metG"
                     /locus_tag="LR110_002194"
     CDS             complement(87..2096)
                     /gene="metG"
                     /locus_tag="LR110_002194"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBL4823141.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2364..2642
                     /locus_tag="LR110_002195"
     CDS             2364..2642
                     /locus_tag="LR110_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823142.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2739..3560
                     /locus_tag="LR110_002196"
     CDS             2739..3560
                     /locus_tag="LR110_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL4823143.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3845..4855)
                     /locus_tag="LR110_002197"
     CDS             complement(3845..4855)
                     /locus_tag="LR110_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4823144.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4867..5820)
                     /locus_tag="LR110_002198"
     CDS             complement(4867..5820)
                     /locus_tag="LR110_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4823145.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5810..6880)
                     /locus_tag="LR110_002199"
     CDS             complement(5810..6880)
                     /locus_tag="LR110_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4823146.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6886..7929)
                     /locus_tag="LR110_002200"
     CDS             complement(6886..7929)
                     /locus_tag="LR110_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4823147.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7930..8871)
                     /locus_tag="LR110_002201"
     CDS             complement(7930..8871)
                     /locus_tag="LR110_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4823148.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8955..9302
                     /locus_tag="LR110_002202"
     CDS             8955..9302
                     /locus_tag="LR110_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBL4823149.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9372..11033)
                     /locus_tag="LR110_002203"
     CDS             complement(9372..11033)
                     /locus_tag="LR110_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4823150.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3104 a         2268 c         2028 g         3663 t
ORIGIN      
        1 taataagaag gaatttacga aaattgcttc tcaaatttcg gaaaaagggg gtatgacaca
       61 actagtgtca tacccccttt gaatttctat gcgatttctg agccatttgg catggatttt
      121 ggtgcttcga ttacttgtaa agagccatct ggtgcttctg cagaaaggat cattccttga
      181 ctgatttgtc cacgcatctt tcgtggtttc agatttgcta caatgacgac ttttttcccg
      241 atcaattcgc ttggatcagg ataaaattca gcgattcctg acaagatttg acggtcttgg
      301 ctgtctccag catctagtcg gaattgcagc aacttatctg ccccttctac tttttgacag
      361 ttgatgactt ctgctacttt tagttctact ttttcaaata catcaaattt gatttgtttt
      421 tctttcgttg aaacaagttc tgtttcttct ggatcccatt tgactgtatc ttcgtttgtt
      481 tgagtacctt cagacatttt ttcttgaata aaagcaactt cttcttccat atctaaacgt
      541 gggaagattg gtgtaccttt tgcaactact tttgttcctg aaggaaattc gccaaagtgt
      601 aatccttcaa gattcattgt ttcagagtcc aatcctagtt gattgaagat cttctctggc
      661 gtttctgtca tgattggctg aagtaatatc gctgcgatac gcaaagattc agctaaatgg
      721 atcatgacac tattcaattc agctttcttt tcttcgtctt ttgctaatac ccatggttct
      781 gtttcatcaa tgtatttgtt tgcacgagaa atcagcaccc agatttcagc caatgctgta
      841 ttgaattcca ttttttccat tgcatcatgg taacgtccga caacgttagc agccgtcgta
      901 gacagttcac tatcaaatgg agtgacaagt gaggcatagt caggaacgag tccatcacaa
      961 tatttgttga tcatagcaat cgttcgattc aataagttcc ctagatcatt tgccagatca
     1021 taattgacac gggaaacaaa atcttctggc gtaaagacac catctgaccc aaacggaaca
     1081 gcacgaagca aataataacg gagtgcatct aaaccataac gttccacaag catttcagga
     1141 taaacgacat ttccttttga tttagacatt tttccgtctt tcatcaatag ccagccatgc
     1201 ccaaatactt ttttaggtag aggcaaatca agggccatca gcatgattgg ccaatagatc
     1261 gtatgaaagc gaacgatttc tttaccgacc atctgaacat ctgcaggcca gtatttttcg
     1321 aatagacttt catcttcgga gccatatcct agtgcagtaa tataattgga taatgcatcg
     1381 atccatacat acaccacatg tttcggatca ctcttgactg gtactcccca agtaaaagtt
     1441 gttcgtgaaa ctgcaagatc ctctaatcct ggtttgataa agttgttgat catttcgttt
     1501 ttacgagatt ctggctggat gaattcggga tgttcttcat agtattccaa tagacggtct
     1561 gcgtatttgc tcatgcggaa gaagtatgat tcttctttta ccaattctac ttcgtgtcca
     1621 cttggcgctt taccaccgat cacgttacct tcgtcatctt tgtacacttc tgcaagttgt
     1681 gtttctgtaa agaattcttc gtcagaaaca gagtaccagc cttcatattc acctaagtaa
     1741 atatctccct gttctaataa acgatcaaat attttttgaa cagctgcttt atggtaatca
     1801 tctgtcgtgc gaatgaattt atcataactg atatcaagcg ttttccataa tttcttgaca
     1861 tcagccgcca ttttatctac atattcttgc ggttttacac ctagttcttc tgcttttgtt
     1921 tcgatttttt gaccatgttc atccactcca gtgagataaa acacatcaaa gcccatcaac
     1981 cgtttataac gagcaattgc gtcacatgca atcgtagtat acgagttgcc gatatgaagt
     2041 ttgccgcttg gataatagat tggcgtagtg atataaaaag tttccttttc tgccacgggg
     2101 ctcttcctcc tttatcgcat gatgcgaatt cgtttccttg ctagtatacc ataaaaaaag
     2161 catgctgtta gctttttttc aaaatgaaaa agactggata aacgacttct atctacgttc
     2221 aatctatcct ttaaaaggat gaataggttg aataaatgcg ggtttttgtt ttgtttcata
     2281 tctactaaaa aacggctttt tatttctccc ctcacacgtg gtataataaa gactatctat
     2341 cttagtaagg gactggaaca atcatgcaga agaaacatct tttttttaca cttagtattg
     2401 cttttctttc attagcacat ttaatattca gctattttta tattcgtatg tatggttatt
     2461 ttaacctcca tgggcatttg aacagtttta tgacagcagc ttggattttg cgttttatca
     2521 ttgacgtata tatcgttatt tgtggcttct ttgccattcg tgaagagcgg tataaagtat
     2581 tgccgtttta tctgcttttc tttttattca atctcatact tccatttatt tttcatattt
     2641 aaatgataac ctagattttt caagtaaatc cttggttttt tttcagagtc tgctacaata
     2701 aggtaaaaag tacttttact gtcaatggag gtggtcagat gaaccgtttt gcttctaaag
     2761 aagaagaaaa attatattat gaacataaag caagtgaaga agaatttctt aactggtatc
     2821 aaaaacagga acgtccagaa tatgacaagc cctctttgac tgtcgatatc gtactgatgt
     2881 gctataacaa agaagaagat cagctgaaag ttttgttgat ccaaagaaaa gggcatcctt
     2941 atcgaaattc atgggcattg cctggtggct ttgtccaaaa ggatgagtca actggagaaa
     3001 gcgtcttaag agaaacaaag gaagaaactg gtgtggttat ttcaaaagaa aatattgagc
     3061 aactgcatac attcagtacg cctaatcgag atccgcgagg ctgggtcgtc acagtcagct
     3121 acttagcctt tattggagaa gaaccgctaa tcgccggaga tgatgccaag gaagtacgct
     3181 ggttcacact agaacgtcat agtaacaaga tccatttatc tagcggagaa gtggctatct
     3241 cgcttgattt agtcactgga gagtcttctg gcaaagatac tttggctttt gaccatagtc
     3301 aaatcatctt gaaagcattc aaccgtgtag tcaataaaat ggaacacgag ccgcaagttc
     3361 tacaagtttt aggtaaagat ttcacgatta cggaagctcg aaaagtattt gctaaattct
     3421 tagggatcga ttataaaacg atcgatcatt ccaattttaa gaaagctatg ctccaatatt
     3481 tcgatgagat cggtgaacgt cctgtaggaa tcggccgtcc ttcaaaaatc tatcagctca
     3541 agccggggca ccatgaataa ggttgtgagc aaaagattca gacctaatca ttcaagaaaa
     3601 aacgcaaaaa caactctttg tgagttacgt tttttgtttc agaattttga tgtattcgta
     3661 aactttctga ataaaaaatt aacaatagca gaaaaagctc gtacgaactt tttctgctat
     3721 tgttttattg gtttgtcata attagtgagc ttttcttcaa aagaaaaatt cctagaaaca
     3781 aaagtcatta agacttttta ttaacaaggc taaccctaac gctcctatca gtttcattca
     3841 ttttttatcc cttgataacc gaatgtctgc ccattttttc tactacttgg ctctttctta
     3901 cggaattgct ttcttctgct ccgtcggtat tgatcaaaag aaatttttta ttataccatt
     3961 gggacttata acgcaatttt gttattaatt tatgccagtc gtgaatagaa aaatcagcat
     4021 gtgcctcttc ttttggaaaa gattctacaa ataagtagtc atattgtttg accaatttcc
     4081 cagataattg ttcaagataa tcttctcgct ggttcttttg tttcagataa agatccatta
     4141 cttttctctt ttgtttctga taattgcttg cctgatctaa cctgatcttt ctatggtgag
     4201 cggagcggac tttcacattc aatttccgct gtgcatgttg cagcttttct tctgtctggt
     4261 gaaattttgg taaagttact tctagaggtc gagatgtttg gatcaatgtc ttcggatgat
     4321 aggcgatccc gatccattta gctaaagatt ctttttttac tggcgaaaca tcacaaagta
     4381 gtgcgacata aaattcttca ttatatcgag cagaaatcgt agcggagcga atcgatcctt
     4441 caactggccg atgctgattt atcttgatta gttctttttg ttttggtaac tttaagtagc
     4501 cattttccag atagatcgta cgggtttggt tgttggtcgt ataagattgc cacatacttt
     4561 ttttcgtttt aagctttggg aagctggcac gtcccttgaa ataattgcga aaagcccgat
     4621 ccaaattaag ctgtgcattt gctaaagcaa gactgtctac ttcttttaag aatgggtatt
     4681 ctttttttaa tgtagcgggt gtgagcgaaa aattggctac tcctgtttgt tgccaacttt
     4741 tctgccgctt tttcagcaaa tggttatagg taaatcgagc acatccaaaa gtacgaatca
     4801 attgctgttt ttgttcttct gtcggataca tacggaattt aaaagctttt agcatctatg
     4861 tccctcctag ttagcaacca gaagagattc cttttgtcgt tttagttttt ttgcgtaata
     4921 tgggacttcc tgctcagttg catagacaaa atgttctggt gcgatttcac gaagttgacg
     4981 tacttgtccg tcatctggtt caggttgata tttgattctt ctgcgttttc tctcatggtc
     5041 tggatctggt agaggaatag cagataacaa actttctgta taaggatgaa caccgtgatt
     5101 atagatttca tcacttgttc ccatctctaa taacaagccg ttatgcatta caccgattcg
     5161 gtcactaata tgtttaacca tcgacaaatc atgtgcaata aatagatagg tcaattgatg
     5221 ttctttttgg agatcttgca acaaattgac cacttgggct tggatcgaca catctagtgc
     5281 agaaattggt tcatcacaaa tgataaaacg cggttttact gccaatgctc gtgcaatacc
     5341 aatacgctgg cgttgcccac ctgaaaattc atgaggataa cgagtaccgt ggcttggatt
     5401 caatccaaca gttctcaata gatcatcgac tttttttgca cgctcttcag atgatttagc
     5461 tagtccatta acatcaattc cttcggcaat gatatctctg actttcatac gaggattcaa
     5521 cgaagcgtat ggatcttgaa agatcatctg aacatcatgc cggaatttcg tcaaaccttt
     5581 tttatctttg atcttcgtta tatcttgacc gtcgaataat atctcaccat cagtaggttc
     5641 attcaagcga atgatcgtac ggccagtagt ggattttccg cttccagatt ctccaactaa
     5701 accgaatgtt tctccttcat aaatgtgaaa gctgatgtca tttacagcat gtacttcatt
     5761 cttttttcct acattgaaat actgtttcaa tcctttaact tcaagtagtt tatttggcat
     5821 cagcaattcc tccttgaccg taagtatgtt tttgtttctc tttaaagata gcccagcgac
     5881 gttggatttc ttctggcggt gtcactttgg gtgcctgcgg agcaagtagc caagttgctg
     5941 ccttgtgtgt ttttgatagt tcaaaaaatg gtggttcttg ttctgcatcg attttcatcg
     6001 cgaattcatt tcttggatag aatgcgtctc cttttggcgg gttcaataaa tccggtggag
     6061 aaccaggaat agcatacaat cgatcgttcg cactatctaa tgtcggcatt gaaccaagca
     6121 gtccccaagt atagggatgt tgcgggttgt agaaaatttc ttccgatgtt ccaacttcaa
     6181 cgattcttcc tccatacatg acagcgacac gatcagcgac attcgctact acaccaaggt
     6241 catgagtgat aaagataatt gatgtatcga tttttgtctg aatttctttc agcagctcca
     6301 aaatctgtgc ttgaatagtc acatcaagcg ctgttgtagg ttcatcagca atcaggattt
     6361 ccggataaca taccagtgca atcgcgatca cgatccgttg acgctgacct cctgagaact
     6421 gatgcggata actgttgata cgtttctcag cttcgggtat tccaactaat ttaagcaatt
     6481 ctaatgcagc tttttgtccc tctttttttg aaactttatt atgctttcta agggactcag
     6541 cgacttgctt cccaatgggc atcgtcggat ctaaagaggt cattggatct tggaaaatca
     6601 tcgctatttc tttaccacgg attttttgca tctctctttc tgattttcca acgatatcgt
     6661 tacctttaaa gaggatttga ccagcatcta tattggcgtt gcttgacagc aagcgcatga
     6721 tgcttctggt cgtcactgat tttccacttc cagattcccc aacgatcgcc aatgtttctc
     6781 ctttattcaa atgaaaattg acaccgcgaa tcgctcgtac ctttccagca tacgtatcaa
     6841 aagagatttc taagtctttc acttctaaaa ttttcgtcat cgtcttcact ctttcatttt
     6901 aggatcaaag gcatcacgca atccatcagc aagtaagtta aagccaatca tgataaccga
     6961 taaagttact gccggaatcc ataatagata aggcagatac aggaatgtct tgtacccttc
     7021 gctcaacatc gttcctaatg aagctgacgg aggcgtaaga cctaaaccga taaagcttaa
     7081 aaaggcttcg aagaaaatag cagatggaat actaaacatc atttgaataa taatgacact
     7141 agagatattc ggcaaaatat gtttgaatgc aattttcatt cttgattcac ctaatgtcaa
     7201 tccagctaaa acatattctt gatcttttaa tttcattgtt tgtgcacgaa cgatacgtgc
     7261 cattggtatc cagtttgtga tggccatagc agccacgatt gaaaaaattc ctggttcaaa
     7321 aacgaccaac atcaaaatca tgacaacgag gtttggaatt cctgataaga tttccaaaaa
     7381 acgttgcatc acgttatcga cacgtccgcc taacaaacca gaaaccaacc cgtaagctac
     7441 accaatcgtt acatctagta atgcagcaat aaatgcgatc agcaacgaga ttctcattcc
     7501 cataaataag cggcttaata catcacgtcc caaaccgtct gttcctaaaa agaaatttac
     7561 gtttgaaggg acgtttgctt gagcatattt atcaacaagt tcaccaccaa ccattgcttt
     7621 tccattcaat ccatcgatat tgattcccgg aatacgagga ggcaaattga tataagccac
     7681 gttttgggca gttggatcat gaggagaaac aaagattgtg atcacagaga tcaaaatgat
     7741 gaccaatagt aaaaccattg aaataacggc agctttgttt ttctttaaac ggcgccatga
     7801 atcttggata aaattcaaag aaggtgcagt aatctgttca cgttcttctt cggtattttt
     7861 ttgtaatgga agaaattcat ccgcagggat atctgcaact gtttcataac gctttggtat
     7921 catttccatt tatcctcggc tcccttctga aacacgaatt cttggatcaa ctagaccgta
     7981 caatagatcc acgatcaaaa taacaaaaat cagcataaat gagtaaagga tcgttacagc
     8041 catgatcgta ggataatcat ttgttgtgat tgacttaacg aattgttccc cgataccagg
     8101 aatagcaaag atattttcta ccaccagtga tccggtcatc aatgctacag ctaacggtcc
     8161 taataaagtc atcaatggaa tcaaactatt tcgaagacca tgtttaaagg cgatttccca
     8221 acggctcaat ccttttgctt ttgctaattc cacataatca ctatgcagta cttccaccat
     8281 ttccgtacgg ataaaccgag cagagtcagc aagcggcgac atggccaacg caattgttgg
     8341 taagattgtg tacacaaagc catcccattt tgcgattggc aaaacttgta atttgattgc
     8401 aaagatatat tgtaataaaa cagcaaatac aaagtttgga atggaacgac ctaatatcgc
     8461 tacgagtgta gaagatgtat ctgcccatgt attttgcttc atggcagaga tcgttcctaa
     8521 aatcgtccct aggactgttc caaagataat tgcctgtaac cctaactgca aggatggtcc
     8581 gattcttcca gctaacaaat gtgcgactgg ctggttttta aattggaagg aaattccaaa
     8641 atctccttga agcaaattag aaagataaat cccatattgt acgataactg gtttatccaa
     8701 accgacttgt ttatttagca tctcgattgt ttcaggactc aaacgttcct gattcgtata
     8761 tggcgtgccc ggcagcaatt gcatcaagaa aaaggtgatt gttgcaatta accaaagcgt
     8821 gatgatcatg aaaaagacac gttttaatac atatttgata taactattca ctaatgtttc
     8881 cccctagctt ttgacttctg acagatgttt cgctacatta ttagataatg aaccaaaaat
     8941 aaaggagcaa tcaaatgaaa aaaaaatcta tcccctacgc agtagctttt ctactgattc
     9001 tggttatttt aataaaaaat ctcatcaacc attcctttac actcattcaa ttatctaatg
     9061 atctattctt atggtcattg ccgtttctga ttatcggcgg ttttttatgg gtcttttctt
     9121 cgggtttctt cgatcatttt cagcgatcgg tccatcttgc acgaacaagg aaccgaaaga
     9181 aaaaaccaga attttcctcg ctctcgtctg ccagctacgg tatgtacagc ttttggctga
     9241 tcattgccgg aatactaatt gctctttctg ctattttcat gcttttttct ttgttaggat
     9301 aagtgaatat gagaacgagg ttgcgacatt tgccgcagcc tcgttttttc tcatgctcac
     9361 ggtattaacg attattcttc aacgtatgtc catttgtaat cccatgaagc gcctgccgca
     9421 tggtgaacga tacctttgat tttttcattc actaggtgac cttctgcttt ttggtacacc
     9481 ggaacgacac ccatatcatc agagatgatc ttgattgctt tttggaaatc atcccaacgg
     9541 gcttgttcat cgccagcgtc tgttgatgaa gctgctttaa ctgcttcgtc atattctgca
     9601 ttgctccagc gaccacggtt gtatgaatta cctgtgacaa acaaatcagt aaagctgctt
     9661 gggtctgcat agtcagcagc ccatccgcct agtactgcgt caaaatcacc cgaagttgag
     9721 cggtcaagac gtacagagaa tggaacaggt gttggtttga ctgttacgcc atccaagttt
     9781 tcttggattg agttttgaac atattcgatt acttttttcg atgaatcatc atcagaagcc
     9841 atcaaatcaa attctaaaga atcaacgccc aattcttttt tcgctttttc ccaatattct
     9901 tttgctttat ctttgtcata agaaactaat tttccagctt cttttgcaaa gtcttcgttc
     9961 gttgttggac tttttaccat atctgatggg atcaatcctg tagagacaac tgatccgtca
    10021 cctagtactt gttttactaa agcttcacga tcgatagaat aagaaatagc ttttcttagg
    10081 ttcacattgt tgaatggtga attcttttca cgttgattga attcgatata gcttgtccgc
    10141 gcttctttaa cagaagtata agctggatcg tttgcgtttt gctgtgctaa ctcaccagac
    10201 aagatcacgt catctgcttg gccatctttg aataagttca atgcagtaga agattctttt
    10261 actacactga ctttgatttc agaaagtttt actgtatctt tgtcccagta atgatcattt
    10321 ttcttataag tccattctgt atctgttcct ggtccatcaa aatcttctaa tgtgaatggt
    10381 ccattataaa cagctttatc acttgcagat gcataggcat ccccattttc ttccactacg
    10441 gattgttttt gtgggaaaaa tgatgggaaa gcaagcagat attggaaata tggtgtttgt
    10501 ttcgtcaatt tgatttctaa ttcatagtca ccaactgctt tgatccctag ttctgattta
    10561 tctttctttc cagctgtaat gtcttctgca ttttcaactg gcgcatacag gtaagcatat
    10621 tcagaagctg tagctgggtc tactgttcgt tgccagccat aaacgtaatc cgctgctgta
    10681 acaggatcac catttgacca ttttgcgtct tctcgtaatt tgattttgta agttaatccg
    10741 tcttcactga cttctgccaa ctcgctagct cctgccggct gtggtttgct gtcttcatca
    10801 agacggtaaa ttccttcata aacgttattc aatgctgtaa aactgattgt atctgttgca
    10861 actgataagt cagctgtagg catttcttga ggcacgacta agttaaatac gcctcctcca
    10921 tctgctgaac cgctttttgc tgaactactg cttgtggctg aactgccgcc ataacatcca
    10981 gcaagtacaa ggccgcatac cgcaaccaaa ccaaatgtaa atttcttttt cattttacgt
    11041 acccctttat tggaattttc aga
//
LOCUS       DAFFSV010000100        10887 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFFSV010000100 DAFFSV010000000
VERSION     DAFFSV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.100"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            142..720
                     /locus_tag="LR110_002204"
     CDS             142..720
                     /locus_tag="LR110_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL4823151.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            720..1649
                     /locus_tag="LR110_002205"
     CDS             720..1649
                     /locus_tag="LR110_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4823152.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1646..3382
                     /locus_tag="LR110_002206"
     CDS             1646..3382
                     /locus_tag="LR110_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL4823153.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3445..4281)
                     /gene="pheA"
                     /locus_tag="LR110_002207"
     CDS             complement(3445..4281)
                     /gene="pheA"
                     /locus_tag="LR110_002207"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBL4823154.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4278..4787)
                     /locus_tag="LR110_002208"
     CDS             complement(4278..4787)
                     /locus_tag="LR110_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBL4823155.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4780..6075)
                     /gene="aroA"
                     /locus_tag="LR110_002209"
     CDS             complement(4780..6075)
                     /gene="aroA"
                     /locus_tag="LR110_002209"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL4823156.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6088..7179)
                     /locus_tag="LR110_002210"
     CDS             complement(6088..7179)
                     /locus_tag="LR110_002210"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBL4823157.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7196..8362)
                     /gene="aroC"
                     /locus_tag="LR110_002211"
     CDS             complement(7196..8362)
                     /gene="aroC"
                     /locus_tag="LR110_002211"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBL4823158.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8364..9428)
                     /gene="aroB"
                     /locus_tag="LR110_002212"
     CDS             complement(8364..9428)
                     /gene="aroB"
                     /locus_tag="LR110_002212"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBL4823159.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9461..10483)
                     /gene="aroF"
                     /locus_tag="LR110_002213"
     CDS             complement(9461..10483)
                     /gene="aroF"
                     /locus_tag="LR110_002213"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBL4823160.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         3028 a         2398 c         2003 g         3458 t
ORIGIN      
        1 aactatgaca aaaggaataa tcttagcatc tagaccaaag atcgaaatgg tgaggtttct
       61 ttaaaataaa aatatccttg cattttctcc tagaaataag tatttttaaa ttacaagact
      121 attcttaagg cgggtaaaaa aatgccaata cgcgtaattc ttatcgatga tgaacagacg
      181 attttagatg gaatgaaaca gttgatcaat tggtctgagc acgggtttca attaataggg
      241 actttttcca gtccgctgaa agcgcttgat ttttctttgg tacatgcaat cgatatcgtg
      301 gtcacagatg taatgatgcc tgaattaaat ggaatcgaat tatcgcggct attaaaacag
      361 caacaacctg aaacacagat ccttatcctc tctagctatg atgaatttga tatggttaaa
      421 gattctttta aagaaggcgt cgctgattat cttttaaagc ctcgattgag tcctgatttt
      481 tttctaaatt ccttaactgc cgtttcacaa aaaatcaaaa aaaagcccaa caaaagccca
      541 atcaccaatc gttatgagca gatctgcgag aatctttcac agcagattgc tggacaaact
      601 cgtccattgc ttgaccctct tctttttgaa cacaccttgt ttttctgtta tataccgaaa
      661 aacggccatt cagttcaaaa caaggctcta aacaaaaaca gtcgatcacc cattcattga
      721 tggatagcga cgattcgttt actatttatc ctgtttctac cttttcggaa gaatccggct
      781 atgtaatcaa tactgattcc aaagatacac ttaggaagtt tgttcaaaag tttcgtcaag
      841 aagtttttcc aggtgatttt tttgttgttt ctgattcttt tgcaatccaa gatatcaatg
      901 aaatattcca agagatcaga aattgcacca aaggacaacg tttctatcat aagggaaaga
      961 accttgctta tcagcatgaa cttcttccgt tattaaccaa ccacgagcag caagcaagta
     1021 gttatttaac aaaaatcgtt cataaagact acgctttagc tgtcaaagaa cttgagaatt
     1081 tcttcacgac gatattggaa cagcctattg atccagctta tttgaaacat gaggcaatca
     1141 atcgtttcta tgcattactc aacgctttgg ctgacgagca tgttgggaat gaagaacttt
     1201 ctttgctcaa gatcgccctt ccttctttat tagccgatgc tgagtatttc gaagattttt
     1261 ctattcttgt caaagaaaca cttgagaaac tcacccactt ttctgcaaat gcaaaaaaag
     1321 acaatttcga tatcttgact ttgatctacg agttcgtcca ggaaaattat gagaaagaaa
     1381 tcaatttaca acttttatcc gaaaaatatc atttttcata tagctatctc tcagccatct
     1441 ttactgaaaa atttggtatc aacttttcaa aatatctaaa aaaagtccgt atcagtaaag
     1501 ccaagctctt tttgacggaa acacggctta ctttgacgga aatctgtgag aaagtcggct
     1561 ataccgaact gggttatttc tccagagttt ttaaagaaga gacaggaatg acaccttctc
     1621 aataccgtaa aggaactttt gtaaaatgag aaaaaaaatc aaagagcatg aactctttgc
     1681 caaacttctg ttcattttgt tcatcggtct ttttctgcaa gccatcgtca tttctttgtt
     1741 tatttatcat cgatcaaggg atgcctatat ccagctgttc aatcaatcga atgatgtggt
     1801 actaaaaaag attcaatctg aatttgaaag tttgaatgat acaatcgaga atacattagc
     1861 cgcttttgat tctaatccag ctgtcaaaag ctatttttcc aatgatccag cccaacatat
     1921 ggaacagctt cagcaactaa gaacaatcca aaaaatgaat gactctcttt caaaaatcca
     1981 tccaatgatc gattacgatg tattgatctt tggagaaaat ggaagaactt ttgtcggaaa
     2041 cgacatgctc actgccgtat ctgccgattc tttttttcaa tctgcgattg ctcagagagt
     2101 gaatgaacgt gctgctgata cacaaatgct ttttgcacac cacggattga ctcttcgcga
     2161 taagaaagca cccagcgtct tctttgtcaa aaaactgaaa aatacattga atcacgttta
     2221 cggatatgct gttttatcga tctcatccaa gcaactagcg aatctttttc aatctgtagt
     2281 aaatcctgag atatcgaaga tctccttcat gaacgaagaa cagacgatca tcgcatcaaa
     2341 tgaagaaaat acaattggga gccgatcaat gattttcaaa aaactaacag taggtgaaac
     2401 aacgatttca aatggacagc agttgacccg tcttcctctg taccgccagg attgtacatt
     2461 actcagtcaa gtcgatcttc attctttatc taaccaaatg ggcgtgattt ttccgattat
     2521 tttgtataat atcgtgacac ttgcttttgc tggctcattg gtatttatgt atttggatcg
     2581 acacacaaaa tcgatctacc gcttgattga ttcgctaaaa cggctagagg aagatggctt
     2641 aaaagctact gttcctgtac aaggaagcca tgaagtccaa ttgctaggga atacgatcaa
     2701 tcaattgcta agtaatgctc agcatcatta tgaacagacg atccaagacg aaaagaagaa
     2761 acgttcgctt gaaatccaaa ctatgcaagc acagatacag ccccatttta tttataatac
     2821 gctgactagc ttgaaatttt tgatttggca gaaaaaaaca gatgaagcag ttcaaggaat
     2881 cgacagtttc attactttac taagaagtac gattgggaaa aaagaagaag tgattccagt
     2941 caaggaagaa atcaaaagcg tacaaagtta catcgatctt ttgagtatgc gatatggcga
     3001 aagaatctcg gctaaaataa tgattcctga tgaactgcag tcattatcta tgccgaatat
     3061 gatccttcaa ccaattatcg aaaacgccta tcttcatgct tttcacaata aaacgcaagg
     3121 attcatcacg gtgtacggca aaacaaaaaa tcagcttctt ttattcgaag tgattgataa
     3181 cggcgatggc ttcgatactg aaaataccaa acaaaaaaat gatcattttt caggaatcgg
     3241 catcagccat gtagatgagc ggatacgcct aatgtatggc gatgcatatg gcttaacgat
     3301 ccaatcagtg attgggacag gaacgactgt aacgatttgt ctaccgcttc ttcctaaaaa
     3361 tcaatcagaa tatcaaaaat aaagaatagc caagcgctga tttttttatt ttaagttaaa
     3421 taaatcaatc agtattcttg actttcatct acgcgtctct tctactgtca gcactggata
     3481 acagcccaat atttgacttt ctcctccgag catcttgatt tcttggagcg cattctctac
     3541 taatgtcatt ggacggtcca acaacaaatc gatcacgaaa aaatattctc ccaagctagt
     3601 tttcaatggt cgggattcaa tcttacttaa gttgatttcc cgccagccaa aagctgccag
     3661 cattttatga agcatccctg gccgattagc aggtagtgtc aagatgacac tcatttttac
     3721 tggagctgac tgctggctgg tcatctttcg gtcaccgacg atccaaaaac gtgtctggtt
     3781 caactcatta tcttggatat tctcagctaa gatctccaat ccaacatgct gtgctgtttc
     3841 taatgaagca atcgctgctg cgtcttcttt cggatgttct gctacataca ttgctgcagc
     3901 agttgttgat tctgttgcta ctaaaggaac attaggataa tgcgtttcta aaaactgttg
     3961 tgattgtgct agtgcttgtg gatgtgaaag gatttttgta atctttgttg ctggattgcc
     4021 taaaagctgc tgcttgatag gaagaacgat ttcactttta acttcgactt ctggatgttt
     4081 ggaaagaaga tcgattgtgt gatgaacagc tccttccaat gaattctcta caggaacgac
     4141 agccagatcg acttgtttgc gaaacaaagc gcgcaaacaa acagggatgg aagcatacgc
     4201 agtcaattgt tctgtaggaa atagctgaca agccgcctga taagtgaatg aactttctgg
     4261 ccctagatag ctgacgttca tatgcttttg atcctttcta taatggtatc gatgatcttt
     4321 tctggtgttt gattggtcgt gttgattcga tgtgtcgcac tttcttcata ccaagaaagt
     4381 cgttgtgcca ataatgcttt tatttcttct cttgtttttc caagagctaa aggtcgaata
     4441 gacgtttcat cttgttggat acgatcaact agacagtccg cttctgcctc taagtaaaga
     4501 acaatcggct gtgtttttaa aaaacgccga ttttctgcac tttggacaat ccctccacca
     4561 gtcgcgataa ttttgtcttc atttgacagc tttcttaaaa tatctgactc cacttttcga
     4621 aagctttttt caccgtaata gtgaaaatat tcggcaatcg acaattggat atgttcctca
     4681 atctttgtat ctaaatctac atactctttt tgtaaacatt gagctaaccc tttaccgatt
     4741 gttgtttttc cagcgcccat gaaaccaatc agcaaaatgc tagccatgag cttctcctcc
     4801 tgacacaagt tctgctaaat cgtcaaaaaa tgccggataa gagattgaaa cagcttcagc
     4861 tccttctagt tcgcaaggtc catcagctat caaagcagct acttgcagca tcataccgat
     4921 tcgatgatcg cctctgctgg acacttttgc gccatgcaga ggtgtcggcc catggatgat
     4981 cagaccgtct gctgtcgctt ttatctttgc tcccattttc tgtaattctt ctgccaccgc
     5041 atcaattctg ttggtttctt ttacttttaa ttcttctgcg tctttgatga tcgtgattcc
     5101 ttctgcttga gtagccacta aagctagaat gggcaattca tcaatcagac gcggaatgat
     5161 ttctccacca atcgttgctt tcttcaaatg actcgtcttg acacttaaat cagccgattg
     5221 gttctgttca ttgtgattcg tctgggtaat cgctgcgccc atctcctcca acacatctaa
     5281 gataccagtt cttgttggat tgaccccgac attttttaac aacagttggc tttccggaac
     5341 aagaagtcct gctgctagaa agaaagcagc tgatgaaata tcacctggaa cagttatctg
     5401 ttgaccagtt aatttttgcg gtccggtcac tatgatcgtt ttatcttcca ctgtgatcct
     5461 tccgccaaat tgacgaatca tttcttccgt atggttacgg gaacgttctt tttcaacgat
     5521 tacggatgtg ccttctgctt gtaatgccgc aaacagcaaa gcagatttga cttgcgcact
     5581 ggctacaggc atattgtatc ggataggtga taaagagcgg gttcctgtca cagtgattgg
     5641 tagatactgg tcattttctt ctccttgcaa gtcagcgccc atctcgcgta aaggttccat
     5701 cactcgcccc attggacgtt tagacaacga agcatccccg aacagagtag tcgtaaaagg
     5761 ctgtccagcc aaaataccca tcaataaacg tatggtcgta ccggagtttc ccatgtcaat
     5821 tgattcggtt ggttggacaa atgagtggct accgcgacca tgaatcttga tcacctcttc
     5881 ctcttcttcg atctcgacac ctaattgttg gaaggcatgt aaagtactca gacaatcttc
     5941 tgcgcgcaaa aaattttgta tagtcgtcgt tccttcagca attgctccaa acatgatgct
     6001 gcgatgggaa attgatttat ctgctggtat cctaacagtc cctcttaatc catgtatttg
     6061 ttgtaataat tgcatttatc cagcctccta tttgatccag caatgataac tggtattaga
     6121 cataatgatt ctttttgctt gtaacaaatc ctgttggttt ttaaaactga tttgcagtat
     6181 tcctaaaata tcttccctgg tctcttggat cttcaaatta atcaaagaaa ctcccgcttt
     6241 tccgatcagt ccagttactt ctgcaataac gccaggttca tctggcacat cgataaataa
     6301 atcaaaaaag gcaggaatcg cgccttgctt atggacaggc aactggtctc gactttcttt
     6361 tgcccgattg aaaaaagaaa agatttccga ttggttctca gtcatgatcc attctgctat
     6421 ttgtgtcatt tcttgttgcc aatctgccaa tcgttccaat aatatctgct tattgctcaa
     6481 caaaatatct gtccacatga caggatctga ggaagcgatc ctagtgatat ctcgaaagcc
     6541 tcctgctgcc agttgtttgg ctcgcggata ctcttgattg aattgttctg cctgattgac
     6601 taagccagct gccagaatat gtggaaaatg acttagcatt cctgttatac ggtcatgttc
     6661 tcttgctgta agagttacat attttgcttt ggttccgcaa aatagttctt gcagtttttc
     6721 aacatctttt ttcgtctttt ccaaaggtgt aaagatgtaa taggcatttt caaatagttt
     6781 agcatctgct gcctgtatac ctgatttatg agaaccagcc attggatgtc caccgacaaa
     6841 ccgaagattt ttctgctcag cggatgtcac gacttgctgc ttcgtactgc tgacatccgt
     6901 aatgatgacg tgatcagaca agttgattct ttcaaggaca tcaagataag ctaaggttgt
     6961 acggacggga acggctaata agataacgtc tgcttgttct gcacctgttt caaaatcact
     7021 tgcaacgtaa tcaacgatct gctgttctac tgcttgctca cgggtttttg actgactatc
     7081 ccaaccaact actatcagtt ctggattggt ctgacgaata caaagtgcca tagaagcacc
     7141 aatcagtcca agccccacga taaaaacatg ttgtgccatc ttatttgccc cctttttagt
     7201 aatgtttcaa gtagtcacgg tactccttca ctgcagcttt caactcaaca agcgaatcac
     7261 ttgggaattt tgacaatagc tctgtcgcta ccacagtggc tacaacattt tccgcaacga
     7321 cgctagctgc aggaactgct gtactatccg aacgttctac acttgctttg taaggttctt
     7381 ttgtatcgat atcaacactt tgaagtggct tgtacaatgt aggaatgggt ttcatgaccc
     7441 cgcgaatgac aagtggttcg ccattggtca ttccgccttc gaagccgcct agattattgg
     7501 tacgtcggaa atagcctctt tcttcttccc ataggatctc atccatcact tctgagcctg
     7561 gtaatgaacc agaagtgaat ccgaccccaa attctacgcc tttaaaggca ttgatactaa
     7621 ccacagcttg tgcgattttg gcatctagct tcgtgtccca ttgtacgtag cttcctaatc
     7681 cagctggaac gcctccaacg agcacttcta caattcctcc gatcgtatct ccatttttct
     7741 ttgtctgatc gatcaaatct tttattcttt gctcaactgc tggatcaaca actcgtactt
     7801 ctgacgcttc agaaatttct ttgatttctt gaatcgagat atttccaggt atttctgctt
     7861 ggattccacc aagacaaatc acgtgtcctg ctacgatgat gtcaagttct tccagaattt
     7921 tcttggcaat tgcgccgatt gctacacgca tggtcgtctc tcttgcagag gaccgttcaa
     7981 gcacattccg aagatctcga tgatggtatt tcataccgcc taccaaatcc gcgtgtcctg
     8041 gacgcggacg tgcaactcgc cgcagtgatt ggtctttttc aggcacttct gctacagaca
     8101 tgacagttgt ccaatttttc caatctttat tttctaccac taaagtgaca ggtgaaccaa
     8161 ttgttttgcc gtgacggatg ccagacgtga tccgaacacg atctttttcg atcttcatcc
     8221 ttcctcctct gccgtaacca gcttgtctgc gagcgagttc gtgattgatt tcttcttctg
     8281 acagaggtaa tcctgctggt agtccttcaa taatggctgt taattctggt ccatgggatt
     8341 ctcccgcagt catataacgc atcttacagt cctcctaagt aatctttgaa tgtctctgtg
     8401 ggaacaggaa cgatttttgc ttgcccaatc ttttctagca agatgatatt caattggctt
     8461 cctcttgctt tcttatcatg ggttatcgcg tggaataatt catctggatg acttagatcg
     8521 tacgtaatcg gcagatggaa tttttgaagt atcctttcta aagcagctgt cgatccagtt
     8581 ggagacaagc cgattttttc tgcatgagaa gtgatggcta ccattccaat agctactcct
     8641 tctccatgac tgactacccc atatcctgct gtattttcaa ttgcatggcc aattgtatga
     8701 ccaaaattta aaataagtcg atttccttgg tcaaagacat cttcttccac tacttttcgt
     8761 ttgatttcca acgtagcagt aatgattgga acagcatgtt ccagaagatc tttttcgtct
     8821 ttcagctcgt tcagctgttt ccaaagcgtt gcatctgcta tagctgctga tttgatgatt
     8881 tcagcgattc cttctcgtaa acgacgtacc ggcaaagttt ttagaacctc aggatcaatc
     8941 aatacaccag ctggctggct aaatgttccg acaaggtttt tcgctttgtt tgtattgact
     9001 gctgtcttcc cgccaatact agaatctact tgtgcaagta gcgtagtcgg aatttgaacg
     9061 aaatggatcc cacgcatgaa cgtcgaggcc acgaatgctg ctagatcacc caccattcct
     9121 ccaccaagtg caagtatccc atcgctgcgg gtaaaatcat tatctgctaa aaaatcgtat
     9181 aactcagctg cttgttctaa agatttactg gtctcacccg ccggtacgat tgaaaggact
     9241 ggttcaaaat tagcttgtct caattggaac atgatttttt ctgcatatag tggagcaacg
     9301 tttgtatcag tgatcacagc tactttttga ggtctccaaa tcgtttgaag ccatgatcca
     9361 gcctgttcta gtgcgccttt ttcaatatga atatcgtatt tttggttatc aacatgtaca
     9421 gtaagcattc gtttcacttc tttcttttaa aaagctggaa ttaatcgagt gtctgaatct
     9481 tttccattgc ttctttcatg atcgcaactt ctttcatcat gcgtgagtaa gtcttttcat
     9541 tcaacgattg gggaccgtct gaccaagcat ttgcaggatc aggatggatc tctacgatca
     9601 tgccatctgc tccgcttgcc acacccgcac gagccattgg tggtacaagt tcccaaattc
     9661 ctgtaccatg acttggatca acaatcacag ggaaatgagt taattttttc atcaatggga
     9721 cagcacttaa atcaaacgta ttacgtgtcg ctgtttcgta agtccgaatc ccacgttcga
     9781 taaaaatgac aggagatttg tcttcgactg caatgtattc tgcagcatta agccattcat
     9841 tgatcgtacc ggaaattcca cgtttcaatc caacaggttt acctgtttta ccgacagcag
     9901 atagcagttt aaaattttgc atattacgtg cgccaatctg taggatatcg ctgtactcag
     9961 ccaccatatc gatatgccct tcatccatca cttcagtaat cactttcatt ccaaattcat
    10021 cagccgcttg acggatgtat ttcaatcctt cttcttctaa accttggaaa gcatatggag
    10081 aagtacgtgg tttgaaggca ccacctcgca gaatttttgc gcccccggct ttggccattc
    10141 gcgcacattc tctgatttgg tctaatcctt cgatagaaca aggaccagcc atcgtcacga
    10201 aactgccgtc accaattttt actccgtcta catccacgac tgtattgcca ggatggaatt
    10261 ctcgagaagt caatttgtat gtgtttaaaa tacgcacagc attttctact ccttcatagc
    10321 gtgtaaatgc tccttcttgt aaacttcgcg tatcaccttt tagtccaatc actactcgtt
    10381 ctgttccttg atcgattgag actttgaatc cttctttttc gatatgatcg acgacttttt
    10441 ttatttgttc ttccgttgct tcttttttca taattactat cattgttttt cccgctttct
    10501 tttgtttact tatctatctt ggctgatttt ctctactttt tcaaggtggt tttacattaa
    10561 ttcctactaa atgaccgtgt cctgttgttc tcattttcgc tccttctttc ctacttatga
    10621 cccagataat caacaaaaaa agatgtccct gtaggaaatt gcctctacag tgaacatctt
    10681 caacgagcct taaaaagcgc tgcaaaaata taaaatagcc actatagatg aatttctcta
    10741 cgtggctgta agtcgtgttt aaaaatcttt agccatcata gatacaaacc tggtttgttt
    10801 gtacccacga agtcatgaat acaaacctat ctaaaatagc taaagtaata cgtattcagt
    10861 tttgtaagtt ttctagattt cataaaa
//
LOCUS       DAFFSV010000101        10629 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFFSV010000101 DAFFSV010000000
VERSION     DAFFSV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.101"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            287..694
                     /locus_tag="LR110_002214"
     CDS             287..694
                     /locus_tag="LR110_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823161.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="LR110_002215"
     CDS             708..1109
                     /locus_tag="LR110_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823162.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="LR110_002216"
     CDS             1111..1482
                     /locus_tag="LR110_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823163.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="LR110_002217"
     CDS             complement(1518..1820)
                     /locus_tag="LR110_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823164.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1916..2710)
                     /locus_tag="LR110_002218"
     CDS             complement(1916..2710)
                     /locus_tag="LR110_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HBL4823165.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
     gene            3356..6163
                     /locus_tag="LR110_002219"
     CDS             3356..6163
                     /locus_tag="LR110_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL4823166.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            6354..6845
                     /locus_tag="LR110_002220"
     CDS             6354..6845
                     /locus_tag="LR110_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4823167.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            6948..7937
                     /gene="manX"
                     /locus_tag="LR110_002221"
     CDS             6948..7937
                     /gene="manX"
                     /locus_tag="LR110_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBL4823168.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            7970..8773
                     /locus_tag="LR110_002222"
     CDS             7970..8773
                     /locus_tag="LR110_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBL4823169.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            8794..9708
                     /locus_tag="LR110_002223"
     CDS             8794..9708
                     /locus_tag="LR110_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBL4823170.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            9791..10165
                     /locus_tag="LR110_002224"
     CDS             9791..10165
                     /locus_tag="LR110_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBL4823171.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            10304..10549
                     /locus_tag="LR110_002225"
     CDS             10304..10549
                     /locus_tag="LR110_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL4823172.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
BASE COUNT         3533 a         1687 c         2141 g         3268 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc
     1741 cacaccttat aaaaatcttt gacaaatcta ttcgaaggat cgaatttaaa ccataattgt
     1801 gtttttcctt ccattaacat ataaagttca cctcaaaaag agtatacgaa caaatgtttg
     1861 tattgtaaat agaaaaagat gagctaaatt aaatgcccat cttctttgat agatattata
     1921 aatctaataa ttcttgtttc tttttattaa attcttcttc tgtaatgatt ccatcatcca
     1981 atagttcttt atattttctt atttctgaag ctgaggaaat tgttgatgta ttggaatttg
     2041 tagcagaaat atttcttctt tttaaattat aaataatttc gtttaaagaa tcacttatcc
     2101 tatcaacagt atctttatca actgcaacat tcaatacttt accaagagta tcaatagtta
     2161 atgtacccaa cagcactcct ctttttttac taatattatt taaattgtct aatataatac
     2221 tttgtacatt attgcctatc attttttgtt gagctacaat taaacgttta ttagttaatg
     2281 cgtatgcata attattgtca tgcttagttg atgagatata attatgcaat cctacaaagc
     2341 agaagattac gtattcgtca ctgttaagtt gatctgatat taagttaaaa tgatgagttg
     2401 tccatttttt agtcattcct ttaccgtagc cgtatttaag acaaaattct accatctctt
     2461 cagtggttct cattctatca ttacctatat catgattgtt ttgtgcagta tgatcagata
     2521 tgtttaattt tcttttttct gatggatcct tatctaaatc ttctaaattc aaagatctta
     2581 ttattttata aggagtagtc ataccgtaac cagctttttt catacaattg ccgcaaacaa
     2641 tttgtttatc tttgatttta tatgaagtta agccaatttt acctccgcac tttgcacatt
     2701 gtttactcac attagttcct cctagatatc agctttctac tactattaac attctatcaa
     2761 tagtgaccat actttgacca tactttttag aaagaataga aaattccaag atatttaata
     2821 atagaagaaa agcactaaca tcatgggttc agaagaattg agagcttatc aggaaacagt
     2881 ataattgaag tgtctgttta tccagtagct caagaagctt aattgaatct tatgattaaa
     2941 gaggttgtga caagactttt gtcacagcct tttttgttta taaaaataaa agttgtagtg
     3001 gaggctacga agtttacttt gtctttttac acttaatatt tttttgccaa tttggaatca
     3061 ggatgttttg ataactatcg ttttcaattg atggaagcta tattgtttgc tatcagtgaa
     3121 ataattatat ggtaaacagt gttctttttc ttgtttccat tataaaaatc gtatcgggat
     3181 ttttcgttct tttaaattga acatttctat ctatccgcgt gttatcaaca aaacaaaatc
     3241 aggtaaatag ttgacgaaac agaaataaat acatagtatt atgttttgaa gcctaagtac
     3301 aatagtctat agaaaaggag cgataccatt ggcaaaacgc attgaccgaa tttatatgta
     3361 tgtcaaagaa aaaaccgctc atttaacaaa ggcagaatat gatcaaggag taacaacaca
     3421 ggaaattgct gaagtgttag ggattcagcg aacaaatagc agtaaagatc tgaatcagct
     3481 agtgcgcgaa ggaaaactga taaagacaga cggacgacca gtacgttaca tatatcaaac
     3541 actcgttacg cacgtaaagc cagttacaaa acatgtagtc agttacaaag aaacgccggt
     3601 ctctgaagaa aaaaatacac tccaaataga tactaaggat atctttgcaa aaatcattgg
     3661 agctaacggc agcatgaaaa attcagtgga acaggcaaaa gctgcgattt tgtatccgcc
     3721 aagaggatta aattgtttga ttacaggacc taccggatca ggaaagactt attttgcaca
     3781 cgcgatgtat cattttgcaa aagcgcatca tgtaatagat gaggaaacgg aactgattgt
     3841 tttcaattgt gctgattatg ccaataaccc tgagcttttg atgagtcatt tatttgggta
     3901 cgcaaaaggt gcttttacag gcgcagatga agaaaaaatg gggattatcg atcaagcgga
     3961 tggcggtatg ctgtttttgg atgagattca ccggttgccg cctgaaggtc aagaaatgat
     4021 cttttacttt atggatcatg gaacttacag tcgtcttggg gaaacaacga aatcccatga
     4081 agcaaatgta cggatcgtag gtgcgaccac agaagaccca ggttcttctc tattagaaac
     4141 ttttgttcgg cgtatcccaa tcaatatcaa acttccggct tttgaaaaac gtcctgcaaa
     4201 tgagaaagtc gatttagtaa aaatcatgat tgcccacgaa gcaaaccgca cgcagcgaaa
     4261 gatttctttg acagaagatg tagcaaaagc attgatcggc agtgtgacct atggaaatat
     4321 aggacaattg aagtcaaata tccagttagt atgtgcacga ggatttctaa atcatatgca
     4381 atcacccgaa atctcgatca caatcgatga tttgacagaa gggattcgta gcggattgat
     4441 tcaattagca agcaaccgca gtgcaatggc agagctttct aaaatattgg agccaaagat
     4501 aactgtagca ccaaatgata cgatcatgaa aatccaatcg gactcctatg agttacctta
     4561 taatctatat gatatcatcg gagataaagc agctttactg aagtcggatg gtttggatca
     4621 agaagcaatc aatcatttca tttctacaga tatcaacatc catctgaagt ctttctataa
     4681 agaccatggt ttttctttta atgcagacag caaattagca gaatttgtag atccgaaaat
     4741 catagaagtg accaatcaga tttatgtaat ggtcaaaaga tctttaccat atgagtttca
     4801 gcaaaacttt atttatgcta tgagcttgca tatcagttct tttttgaaaa aaatcaactt
     4861 aggagaagaa cggcatacga atgataacat ccgtgagatg gcttttgatt ttccagatga
     4921 atatgctgtt gcgaaagaag tgcgtagtta tatcgaaacg tattttcaag tggagattcc
     4981 agaaagtgaa gactattact tgaccgttct actcgtctcg ttacgggcaa atcaagcaag
     5041 cggcagaatc ggtgtagtcg ttgcagctca cggaaacagc acagcaagca gtatggtcca
     5101 agttgtccaa cagttgttag atgcagagaa cgtacgagca gtagacatgc ctttggatat
     5161 ggatccgaaa acagcattgg accgaattga acgcaatgta caagaagtag atgaaggaag
     5221 cggggtcata ctcttagtag atatggggtc ccttgcgtcg tttaattcgc aaattcaaag
     5281 agacactgga attccagtac gtactgttga tatggtaaca acttctctag ttttggaaac
     5341 tgtacgaaag gtctcagtat tagggacaga tctagatatg ttgcatgaat cattgaaaaa
     5401 ttttcgaggt tacgcagaaa tatcttctgt agaatctgtt caggaagcaa aacctattca
     5461 taagaaaaaa gctgttttgg ctatctgcgc atctggcgaa ggtacagcac aaagaatcaa
     5521 agaattgatc gaacgagcgg taaacaagcg tcaagaaaca gaattagaag ttctagcttt
     5581 gtcgattgtg gaagtgaaag aagaactgcc agttatccaa aaagaatatc agatcatagc
     5641 tactactggg attactgatt tgaatatcca agccccattt attcctttag aacgttttat
     5701 tgatcaaaat atcgaagtca ttttggatca gttgctgatg gaatcagaat tggaggaaac
     5761 tgaattcatc tccctagatg aagagtcagc taaaaatacg tgtgttgagt ttatctctga
     5821 taacttcatc tttattaatg gtagtaagtt aatcgatcca atgtggcaat tttctacaca
     5881 aatctctcag acgacgggaa tcggcgatga agaatatgga ttcaaaatca acctagtgat
     5941 gcacacagca ggaatgatcg aacgtattat tcggaatgaa ccattgactg tggaagaaaa
     6001 cgaattgaca aatacaacga atgatcctct ttatagccaa ttggcagcct cagttgttct
     6061 gttggaagat cagataaaag taaaagtgcc gatagaagaa atgtattatc tgctacgtct
     6121 agtccataat cagttagata aaaaagaata cactgtgcca tgaatacaca aaaacgatac
     6181 acttttatag tgtatcgttt ttgtgtattg gttaagaatc ctttaaattc aattagtgta
     6241 tcgtaaaaaa gttggcacgc tttttgctta tagtagtgtg tgctaggagg tggttgttca
     6301 cacagaaatg ataggggttt ctgtgaagta gtgaggaaga aggagttttg cagatggata
     6361 ttcgattggc acgtatagat gatcgtttaa tccacgggca agtcgcaaca gtttggtcga
     6421 agcaaacagg tgttgaacgt atcattgtga tcagcgatgt agtcgctcaa gataaattga
     6481 gaaagtttct actaaaagaa gctgcaccgc cggggatcac agcaaatgta ataaccgtgc
     6541 agaaaatgat tcaaatctat catagtggat tattatcacg agcaaaagtg atgcttttat
     6601 tcaccaatcc ccaagatgtt gaagcggttg tccgctgcgg tgtacagttg aagtcgttga
     6661 atatcggcgg tatgagtttt actgaaggca aatcaatgat taccaatttt gtttcagtaa
     6721 atcaacaaga tattgattcc tttcgttact tgaacagtca agaagtggag ttagagattc
     6781 gaaaagtacc tgctgacagg aaaatccttt tgatggaact actagcgaaa atcaaaaagg
     6841 tgtagcaaga atggttgttg gaaaaggtca aataattctg ttgtaatggt tagcaattat
     6901 taatattttt gaatttgtta caacaaaagt aaataggagg tataaatatg gtagggatta
     6961 tcctagcaag tcatggacaa tttgctgaag gaatcttaca atccggttca atgatctttg
     7021 gcgaacaaga aaacgtcaaa gccgtgattt tgaaaccaag tgaaggtcca gatgacttga
     7081 gagccaaatt ggaagaagcg gttgcttctt ttgataatca agacgaagtg ttattcttag
     7141 ttgatttatg gggcggaaca ccgttcaatc aatcaaacac attattcgaa gagcacaaag
     7201 ataaatgggc aatcgtcagc ggtttgaatt taccaatgtt gattgaagcc tacgcatcac
     7261 gcttttcaat ggaatctgct catgaaattg cagcacacat cattgaaaca gcaaaagacg
     7321 gtgtaaaagt aaaaccggaa gaattagaac cggcagaagc accgaaagca gcagttgaag
     7381 acgcacaacc taaaggagca cttccagaag gaactgtggt cggagacgga aaaatcaaat
     7441 atgtattagc acgtgttgac tcacgtctgt tacatggaca agttgcgaca gcttggacaa
     7501 aagcagtgca accaaaccgt attatcgttg tttcagatgc agtatcaaag gatgatctgc
     7561 gtaagagact aatcgaacaa gctgctcctc caggtgtaaa agcaaatgtt attcctatta
     7621 gcaaaatgat cgaagtagca aaagaccctc gttttggaaa taccaaagca ttgcttttat
     7681 ttgaaaatcc tgaagacgta ttgacagctg ttgaaggtgg cgtagacatc aaagaattaa
     7741 atgttgggtc aatggcgcat tcggtcggta aagtcgttgt aagtaaagta ttgtctatgg
     7801 gacaagaaga cgttgaagcg tttgagaagc ttgaacaaaa aggtgtgaaa ttcgatgtac
     7861 gtaaagtacc taatgattca cgcgataaca tggatgatat tttgaaaaaa gcaaaagctg
     7921 aattagcaaa agcgtaataa ataatattaa aatttatagg aggcttatca tgtctattat
     7981 ttcaataatt ttagtcgtat tagttgcctt tctagctggt atggaaggta ttcttgatga
     8041 atttcaattt catcaaccac tagtggcatg tacattgatc ggtttagtta ccggtaacct
     8101 cgaagctggt atcgtacttg gcggtacgct tcagatgatc gctttaggtt gggcaaatat
     8161 cggtgccgct gtagcaccag atgctgcatt agcatcagta gcatcagcaa ttattttagt
     8221 tttaggtgga caaggtgtta aaggtgttcc ttctgcgatc gcgattgccg ttcctttggc
     8281 tgttgcaggg ttgttcttaa caatgatcgt acgtacaatc gctgttccaa tcgttcattt
     8341 gatggatgcg gctgctgaag aaggaaacat ccgtaaggtt gaaatgtggc acatcattgc
     8401 tgtatgttta caaggggttc gtatcgcggt cccagcagca gcgttgctat ttatcccagc
     8461 acaaactgtg caatcattcc ttgaatcaat gccagcatgg ttaacagatg gtatggctat
     8521 cggtggggga atggtcgtag ctgtaggtta cgctttggta atcaacatga tggctacaaa
     8581 agaagtatgg ccattcttcg ttattggttt cgttgtagct gcaatttctc aattaacatt
     8641 gattgctcta ggtgctttag gtgttgcatt agctcttatt tacttgaacc tttctaaaat
     8701 gggtggttca tcaaataatg gaggcggaag caactctggt gaccctttag gcgacatttt
     8761 gaatgactat taatcttgaa ggaggaaaat aaaatggcag aagaaaaaat caaattatca
     8821 aaaaaagatc gtttagctgt tgcgtggcgc tctaccttca ttcaaggttc atggaactac
     8881 gaacgtatgc aaaacggtgg ctgggcattt tctatgattc cagcaatcaa aaaattatac
     8941 aaaacaaaag aagaacgttc tgcagcatta aaacgtcact tggaattctt taatacacac
     9001 ccatacattg cttctccaat tttaggggtt acattggctc tggaagaaga acgtgcgaat
     9061 ggcgcgcctg ttgatgatgt agcgattcaa ggggttaaag taggtatgat gggtccttta
     9121 gctggtgttg gtgacccagt gttctggttt acagtacgtc caatgctagg tgcactaggt
     9181 gcttcattag ctatgggagg aaatatcctt ggaccaatca tcttcttctt aggttggaac
     9241 attattcgtt gggcattcat gtggtataca caagaatttg gctacaaagc tggttctaaa
     9301 attactgatg acctttctgg cggattgtta caagacgtaa caaaaggtgc ttcaatttta
     9361 gggatgttcg tattagctgc ccttgtacaa agatgggtat caatcaaatt cttaccagtt
     9421 gtatcaactg taaaattaga taaaggtgct tacatcgaat gggataaatt gccttcaggt
     9481 ggtgaaggaa tcaagagtgc atttgaacaa gttaataatg gcttagccct ttctcctgaa
     9541 aaggtaacaa cattacaaga caacttagac caattgattc ctggtttagc tgcattagga
     9601 ttaacattgt tatgtatgtg gttgcttaag aaaaaggttt ctccaatcgt tatcatcctt
     9661 ggtttattcg tagtcggtgt tgttggacac gtcatcggac ttttataatc cataaaaata
     9721 tagctcgggc gttgtcccga gcttttttta tatagtatac tgttcataaa caaatatctg
     9781 gagggagaaa atggttcaat caatcaatac aaaagtagac ttggtaattg atgctacttc
     9841 gtttacgggt ctgacagatt acgggcaaat catgataggt gataatggtt ttgagtttta
     9901 ccatgcgcga gatagacgca aatatatcca aattccatgg gaagaagtgg attatgtact
     9961 agcatcagtc atgctaaagg gaaaatggat tcctcgatat gccattcaaa cgaaaaaaaa
    10021 tggtacatac acattttctt ccaaagaacc taaaaaagtg cttcgtgcga tcagagaata
    10081 tgtagatgaa gatagaatgg tacaatcatt gagtttcttt gatgtagtga aacgtggtat
    10141 tcgtagtatg tttaaacgca agtaaaaaat aaagaaaact tctaagagga ggttttcttt
    10201 atttttttac tttgcagaag aaaatatgaa atagcaaaac caattatttg tcccctttaa
    10261 atcccatatg ctaaaattga ttagcaaaga aggaggttat attatgagtt ggttatggac
    10321 attgattgtt ggagccgtga tcggagcaat tgcaggtgcg atcaccagta aaggaaattc
    10381 gatgggctgt gtaacgaata tcattgctgg actagtcggt tcaagtattg gacaagcgat
    10441 tttcggcgaa tggggaccaa aagctgcagg tatggcactt attccatcta ttttaggcgc
    10501 agtgattttg gtagcagttg tctcattttt ctttggaaaa cgatcataag tttttacaaa
    10561 atagtaaact aaaaggagtt gcagcgagac tgtgactcct ttttggcttt atttatttaa
    10621 aataaaaag
//
LOCUS       DAFFSV010000102        10259 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFFSV010000102 DAFFSV010000000
VERSION     DAFFSV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.102"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            335..1522
                     /locus_tag="LR110_002226"
     CDS             335..1522
                     /locus_tag="LR110_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBL4823173.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1503..2807
                     /locus_tag="LR110_002227"
     CDS             1503..2807
                     /locus_tag="LR110_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBL4823174.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2833..3846
                     /gene="citC"
                     /locus_tag="LR110_002228"
     CDS             2833..3846
                     /gene="citC"
                     /locus_tag="LR110_002228"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBL4823175.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3850..4143
                     /gene="citD"
                     /locus_tag="LR110_002229"
     CDS             3850..4143
                     /gene="citD"
                     /locus_tag="LR110_002229"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBL4823176.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            4148..5053
                     /gene="citE"
                     /locus_tag="LR110_002230"
     CDS             4148..5053
                     /gene="citE"
                     /locus_tag="LR110_002230"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBL4823177.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            5046..6605
                     /gene="citF"
                     /locus_tag="LR110_002231"
     CDS             5046..6605
                     /gene="citF"
                     /locus_tag="LR110_002231"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBL4823178.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6602..7159
                     /gene="citX"
                     /locus_tag="LR110_002232"
     CDS             6602..7159
                     /gene="citX"
                     /locus_tag="LR110_002232"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBL4823179.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMTEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            7152..8015
                     /gene="citG"
                     /locus_tag="LR110_002233"
     CDS             7152..8015
                     /gene="citG"
                     /locus_tag="LR110_002233"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBL4823180.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            8249..8658
                     /gene="tnpA"
                     /locus_tag="LR110_002234"
                     /pseudo
     CDS             8249..8658
                     /gene="tnpA"
                     /locus_tag="LR110_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9295..>10259)
                     /locus_tag="LR110_002235"
     CDS             complement(9295..>10259)
                     /locus_tag="LR110_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL4823181.1"
                     /translation="TRIGTLELKVPRTRDGHFSPTVFERYQRNEKALIASMLEMYVSG
                     VSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIK
                     VREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEYLKERGLQGTELV
                     ISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDIN
                     LAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQE
                     VRRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         3510 a         1609 c         2045 g         3095 t
ORIGIN      
        1 ttcacgtgca tcttcaatat attttaaagc tttatatctg gtgatcccat attttttaga
       61 aatttcacta atattgatat cctcgagata atagtcttca caaattttta caaggagtga
      121 ttcatagtca gagaattcca gatgcatagt atagctccta actttttcaa atagatattt
      181 tttctcatta taccaaaaaa attgaattta gtcttttttt aaatttttaa aaataagcat
      241 cattttttta aaatttaaaa tgaaagggtt tttttatttt aaaaaaagtg ttaaagtggt
      301 tttgtaatta aaaaatgtaa aaaggagtga aggtatgctt gatgaagttt tagaaatcca
      361 taaaaaaaat gtaggtgttt tacggatgga aacaacagaa gccgtgcaag atgatcaaga
      421 tttagcaaaa atgtacaccc caggtgtagc agaactaagt ttaatgattg agaaaaatca
      481 tgctttggca agagaattaa caattagtgg taaattagtg gccgttatta cagacgggtc
      541 ggcggtatta ggtcttggaa atgtaggaaa ccaagctgga ttaccaatcg tcgaagggaa
      601 agcgctgctt tataaaaatt tagcaggtgt caatgcgatt ccattagcca tcgaacaaaa
      661 atcagtagac gagattgtac aaacaattgt caatttgcaa aatagttttg cagggatcca
      721 tttagaagat attgcagcac cgaagtgttt tgagattgaa gaaaaattac aagaaaaact
      781 ttctattcct gtgtatcatg acgatcaaga aggaacagcg atagttgttt tagctggatt
      841 gatcaatgct gctaagatcc aaaggaaaac attaactgaa cttcgagttt tgatcaacgg
      901 catgggagca tctggggttg caacagctag actattaatc gctgctggta tcaaaaatct
      961 aacattggtg gataaacaag ggagattaaa gaagcaagat agtacattaa ataaatatca
     1021 aagagagttg ttggatcata tacatgatac aaataataat gcaagcgttt tggaagatgc
     1081 aatcgttgga caagatgttt ttatcggtct ttcaatagga aatgttttaa cgcctgaaat
     1141 gattggaaaa atgaatgata atccgattat ttttgcttta gccaatccag taccagagat
     1201 cacacccgat ttagcaaaag aatctggtgc aaagctaatc gctacaggtt cttcgaaata
     1261 tcctaaccaa gtaaacaata ttttagtatt tcccggattg ttcaagggac tattactatc
     1321 gaaagcgaat aaagtcgatc tttcattaca acttgaagta gccaaacaat tagctgctaa
     1381 ggtcgcagag cctacggtag aaaaattcat ccctaatgtt tttgatgaag gagtcgcaaa
     1441 cgtcgtttca caggcagttt tggattattt acaaaaacta aaagaaaacg aggataatat
     1501 acatgctgaa aacattaaat agaattaaaa ttggttttat tcctttatct ctttatatca
     1561 ttttggtact cgcgtatctt gcaatgacga taagcaatat cgttccagaa aatgatatgt
     1621 tagtttcttt gggtattatg actgtttttg cttatttatt agaagaaatt ggtaaaacta
     1681 ttcctatttt aaaagattta ggtggtaaag tattagttgt tacatttttg cctgcatttc
     1741 tagtctataa acattggcta cctcaaagta ctgtaactgt ggtttctgat tttatgaata
     1801 ataacaattt cttgatgttc tttatcacac tactagttgt cggcagtatt gcttcaatga
     1861 atcggcaaac attggttaaa gcaacttcac gtattatcat tattttaatt ttatgcgatg
     1921 tgattggttc tttgatagga acaggtgtgg gaatactcct tgggctatct gcttggaatg
     1981 cgtatttctt tatcgttgcg ccgattatgg ctggtggtgt tggggaagga gcgatgccat
     2041 tatcgattgg ttatgcagca atattatctg gtatgggaca aaatgatatc tttgggaaga
     2101 ttctaccgtg tgtgatgttg ggaagtttag tcgcagttct actgtctggt atgctcaaaa
     2161 aaattggaga gaaataccca caattaaatg gtcagggtaa tttgatcgat ggggatgatg
     2221 aacatttaga gaacttgaag ggttcagcta aaccagtcaa tattgaaaaa acattgttag
     2281 caggcgtggt agcaataacg atttattttg ttgctgtcta tgttgagcac ttattaaaat
     2341 cactccttgg cttttctatt ccttcacctg tgatcttatt agtctctctt atgggggcta
     2401 aaatgcttgg ttttatccct aaaaatatcg aagaaggtgg aaattcgttg tatggattca
     2461 ctgttcgtgg gatcacacct cccttacttt ttggcgtagg ggttgcaaaa acagattggt
     2521 tagagctgat cgaagtattt aaaaatcctg caacgatagt aacaatcatt gtgacggtag
     2581 tagctattgt cgctacagct tttatcgccg caaaattttt aaaaatgtac ccagttgata
     2641 ctgccatcgc agtttcatgt tgtagcggac aaggcggtac aggtgcttta gctatcttag
     2701 ctgcgggtga tcgcatggaa ttaatgccat ttgcacaagt tgctgttcga ctagggggtg
     2761 ctatgaccgt aacgttcgct atctttttaa tgggtctgct atcataagaa cataaagaaa
     2821 ggacgtgacc atgtgtttga tcaaacagat atccaacgat tatttttaaa aaataactat
     2881 atcaaagacc aatgggaaaa tcttttgctt gattcaggaa tacaaaagaa gcaaatcgaa
     2941 gattttcaac atttagatca aacattgggt atataccata aagaaaagtt agtaggaaca
     3001 gtatcttatc aaaacaacgt gattaaatat atcgccgtgt ctgaaaaata taaagatggt
     3061 ggacatacat ttaatgtact agtctctgca ataattcagg caatgagtca acaagagatt
     3121 tttcattata ttgttttcac gaaaccgcgc tatcaaaaaa gttttaactt tttaggtttt
     3181 gaaacaatcg tatctacccc aaaaatagct gtattagaaa tgggagatag ttctataaaa
     3241 cagtacctag agaggattcc taaagtgaat ggtgaaaaaa aagcagcaat tgtcatgaat
     3301 gcgaacccat ttacagaagg gcattattat ttagtaaaac aagctgccaa taacaacgat
     3361 tgggtatatg tatttgtact cgaaaaagaa caaaatcttt tttcgacgaa ggaccgactc
     3421 aaattagttc aacaaggatt acaaaaactg aaaaatgtca ttgttctatc aggtggcgag
     3481 tatattatta gccctgcaac gtttcccacc tattttttac gagaagatga tgaaatagca
     3541 aaggagcaaa tggtcgtaga tgctacttta ttcaaagaaa gaattgccac ttatttagat
     3601 atctctgcta gatatgtggg agaagaacca ctttccccaa tgactcgttc atataacgac
     3661 atactggaaa cgattttacc ccctgaaata aaagttcatg tccttgcaag gaagaaaacg
     3721 gaacaacacc aagttatttc tgcttctcaa gttcgtaaag cgtatcttgc tgggcaattg
     3781 gaaaaaataa aatatatggt tccagaaaca acctatcaat acttaaaaaa taaaagggag
     3841 cgttaagtaa tggaaataaa taaatcagcg attgctggaa cattagagtc aagtgatgtc
     3901 caaatcatga tttcaccgaa tacagaagga atagaaatcg atttagacag tcaagtgatt
     3961 gagcaatttg gaaaacaaat caaaaaagtt atctatgaca cactagcagt ttatcagatt
     4021 aaaaacgtaa aactcaaggt agtagataaa ggggcattag attgcgttat tgttgcacga
     4081 ttaaaaacag cgatccatcg ttccttaggt atccaacacc aagaaacacc gtgggaggtt
     4141 taattagatg atgaaagaac gtttgagaag aacaatgatg tttgttccag gaaataatcc
     4201 agcgatgatc aaagatgcag gtatttatgg tgctgactcg attatgttcg acctggaaga
     4261 tgcagtgtct atggcagaaa aagacagcgc aagaatgctt gtggctgagg cattgaaaac
     4321 agtggattat ggtgacacag aattaattgt tcgaataaat gacctgcact cagaatttgg
     4381 acgtgcagac atttttgcaa tggttaaagc cggcgcgcat gtattacgtt tacctaaaac
     4441 agaaacctct gaagatataa cagaagtaga aaaagtgatt atagaagcag aaaactatca
     4501 taatgttccg atagggcgaa cattgatgat ggcagccatt gaaggtgctt ctggtgtatt
     4561 gaacgcacct gcgatcgcca aagcatctga tcgattgatt ggaatcgcca ttggggcaga
     4621 agactatgtg acaaatatga aaaccaaacg atatccagat ggaaacgaat tgttttttgc
     4681 tcgcagtatg atcgttcatg ctgcacgtgc agcaggcatt tccgcatttg acactgttta
     4741 ctctgatgtg gatgatatgg aaggattttt aaacgaagta cgtttaatcc atcaattagg
     4801 ttttgatgga aaatcttgta tcaatccacg gcaaattcca gtagtcaata ctgtttatca
     4861 gccgtcaatc aaagaaatca aacgatcact agacatcatc tcggcaatcg aagaagcaaa
     4921 agaaaaaggt tctggcgtaa tttctttaaa tggaaaaatg atcgataaac caattgtaga
     4981 acgtgcacaa cggacattga tgttagcaaa agcatcacga ttgattgatg aggagggaaa
     5041 tatccatgca taataaacaa agacgtagca ttccatcaag tgttttaaca aagtataaaa
     5101 aagaattcca aagtgtttca ttatcgccac aaacgattca acgttgtgca ccaaaagtaa
     5161 cgactgtaac taatcagaca aataaattga tgcactcgat caaagaagct atagaaaaat
     5221 cgggcttaaa agatggaatg acgatttcat ttcatcatca tttgagagaa ggtgattttg
     5281 tttttaacca agtgatggcc gtgatccgtg aaatgggcat caaagaattg accttagcac
     5341 caagttcttt aacgaacact atgaatgaga tggttattga atgcatcaag gacggtacag
     5401 taactcatat cacaagcagt gggatgcgcg gcagcttagg tgaatttatt tctcatggag
     5461 gaatggacaa tcctgtgatt ttacgttctc atggtaatcg tccacgtgca attgaaagtg
     5521 gtgaattgaa aatcgatgtt gcatttctag gagtaccaac atctgatgat tatggaaatg
     5581 ctaatgcgac tgttggaaaa tccgtttttg gttcattagg gtatgcaaaa gtagatgcac
     5641 aatatgcaga taaagtcatt cttctaactg atcatttagt tccttaccct aatacgccca
     5701 ttagtattcc gcaaacacaa gtcgactatg tcgtggaagt agaagcaatt ggtgatccga
     5761 ataaaatcgg tgctggtgct acacggttta ctaaaaaccc aaaagaatta aaaattgctg
     5821 aaatggtcaa agatgtcatt gttcactcac cttatttcaa agatggcttt tcttttcaaa
     5881 caggaacggg tggtgcagct ttagcagtaa cgcgttactt agctagtgaa atggaagaaa
     5941 aacagatcaa agcttctttt gcattaggtg gcatcactaa accaacggtt gatttattgg
     6001 aaaaaggtct tgttgaaaaa gtgttagatg tccaagactt tgacaaagga gcagctgaag
     6061 gaatggcgcg gcttgagaac caacaggaga tcgatgcttc cttttatgct gatccaagta
     6121 ataaaggagc ggttgttaac caattagatg tcgctatttt atctgcatta gaaattgaca
     6181 caaacttcaa tgtaaatgtt atgactggtt cagatggtgt gctaagaggt gcgattggtg
     6241 gacatcaaga tgcagcaacg gctaaactta ctatcatctc agcgccgtta gtacgtggcc
     6301 gaatacctac agtagtaaat gatgttacta cattgatcac accagggaaa agtatcgatg
     6361 tattagtcac agaagtaggg gttgctatca atccccaacg taaagatcta atagctattt
     6421 ttgaaagaat tccacaaatt cctgttttta caatagaaga attacagcaa aaagcagaaa
     6481 aaatagtagg catcccggaa cctttgcaat tcacagatcg gatagttgcc tatgtagaat
     6541 accgagatgg ttcaatttta gatgtcgttc gtcaggtgaa agaatttgaa gaagagagat
     6601 catgatgttt gatactggag aaattcaaga aatcgaggat attttgatcc ataaagaaca
     6661 acgtgctaac ttgcaaagca acctgctttc caagtattca aatagttcat tgatggtctt
     6721 tacattgaat attcctgggc cgatcaaaaa taatccaatg actgagcagg tatttcgtca
     6781 gggacagcag tcaattcagc aaatgttttt aaaaaaaaag gtggaaatga cgtacacaaa
     6841 agaaatcaat ttgccaagtg gattggactg gtacgtaata gtaacagaag cacctttgaa
     6901 tctcaaaaaa tggttggtca cgttagaaga gcaagacgca ttaggtagac tttttgattt
     6961 agatgtcttg ttcaaaaaag gagagcagat aacaattgtc agtcgccaag ctcttaatca
     7021 tggggaacgt ccttgtttta tttgtgggaa atcagcaaag gtatgtgcac gtaatcgtac
     7081 acatactgtg aaagaaatgc aaaagaaaat cgaacaaata gtagccgatt ccttaaaagg
     7141 agcattgaaa catgcatgat aatattcaaa aaatagcaca agaagcacaa ttagcgatgc
     7201 tttatgaagt tacatgtatc aataaaccag gattggttga tccagtagac cccgggtctc
     7261 atcaagatat ggacattttt acttttctac aaagtagtgt agttttgact ccctattttg
     7321 aagaatttat ccaaacagga ttggaccgac aacatcaacc aattgaagaa acctttatgg
     7381 cgattcgaca gattggttta gaggctgaaa ctgcgatgtt ctcagtgaca aatggtataa
     7441 atacacataa aggtgctatc ttttcattgg ggatttttct tgctatttgt ggccggttaa
     7501 ctatttggaa agttccttgt aaaaaaagtt tctttcaaaa agaaattcaa aaaatgacga
     7561 aaaatctact taacgatttt gggaagattg atcaaacaaa accaaaagca tggacttggg
     7621 gagaatatct atttgttaac catggattga cagggatacg aggggaagcc aaaagaggtt
     7681 atccttgtgt ttttgaccga ggattgcctt attatcaagc atatcaaggg tcgcaacaag
     7741 ataagatgat agacacttta ctttttctat caatggaagt agaagatacc aatttgatca
     7801 aacgaagtaa taatagacat gtttttgaag agtatcaatt acttattcgt ccttattttg
     7861 aattaggggg tgtaaaaacc atacaaggta aaaaatattt ggattaccta aatcagcaat
     7921 ttaaaaagaa aaattggagt attggtggaa gtgcagactt attgatcctt acaatctttt
     7981 tagataaaat catggacaaa ggatggttat gttgatacta taatacatta ttttgtatta
     8041 gtaagctaat aactaaaaaa tggttcaata caaatatttt ataaaaaatg gtagtgccta
     8101 tcctttcaga ttgtagacaa agaaaaattt ctttgtctac aatttttttg ttaatgactt
     8161 cagtcagttt cgtgcgcaaa gcgtgcgaaa caagaaaacc atgcgtttct acacgtggaa
     8221 gaactagcat atcgaaagga gttttttcat gtcaaatgac gataaaagtt tagcccatac
     8281 tcgttggaat tttaagtatc atattgtatt tacaccaaaa tatcgacgaa aagtaatcta
     8341 taggcaatta cgaaaagata ttggaaaaat acttaggaag ttgtgcaaaa taaaaaacgt
     8401 ggaaattata gaagcacatg caacgccaga tcatattcac atgcttgttc aagtaccatc
     8461 aaaaataagt atatccagtt ttatagttat ctgaaagatc gtagtgctgt gctaatccac
     8521 gagcagcacg taaatcacaa atatagatac ggaaataaat cattttggtc ggaggaatat
     8581 tatgtgagta cagtcgaatt gaatcaaaag acaattgaaa aatacatccg tgaacaagaa
     8641 gctgatgatc gcatttgaga tagtataagt aaaagagagt agatgttgca ataacaaaag
     8701 ggtaaaaatg gactacctcc agtaaaatac cgaaaaatag tttaactttt ggagataagt
     8761 ccatttaagg gtctatagaa tgacaatttt tttaggggat tttactatta ttacttcaag
     8821 acccgcttgg attcgcaaag tgagtgaact cttaaaacta cctgatagta ggttgtttta
     8881 agagttatct gaaagtaaaa attgcattta tatttttgat aaatacgtaa atttatatat
     8941 acgtatttat caaagtttta ggccaaagga atattgtgta aatacactag gattgaatga
     9001 atagaccaga agaaattgcg tccaagaaca aaagaagcat gatagtgctc tatataaatt
     9061 gggcattcaa gaatctgaaa tttaaaggta gaattttagt tagactttta gattttaaac
     9121 acaaaaataa ccatacaagc aacaagaagc aaagtgctat attataaatt gtccgtccgt
     9181 ttacttacat aagatcattg gggctatttt taaaagagaa taatagataa taaatgaaaa
     9241 aaaagtcaag tccacaatcc tgtgtaaaat actatacaat gctctcacta ttttttactt
     9301 ggcaaattta atatattttc ttgatgaaga aagccactca tcgtgttgat caataaggac
     9361 tgctccaatt aaacgattgg ctgaaacgca gtttggaaag atacgaataa ttttttctct
     9421 tctgcgcact tcttgattca gtcgttcaat tagattggta ctctttagtc gattgtggga
     9481 atttccttgt acggtatatt gaaaggcgtc ttcgaatcca tcatccaatg atgcgcaagc
     9541 ttttgaatat tttggttgat cgatataatc atgaatcaat cgatttttag cctcacgagc
     9601 taagttaata tctgtgaact taaaaattcc tttaacagct tctctgaaag attttgaatt
     9661 ttttttagga atggtggtaa agatatttct taggaagtga acttggcatc tttgccaact
     9721 tgcgttggtg aaggattttc taatggcaga gactaatcct ttgtgcgcat cagaaataac
     9781 gagttctgta ccttgtaaac cgcgttcttt taggtattca aaaaatgtta tccaggtctc
     9841 ttcgctttcg ccactttgaa tcatgaagcc gataatttca cggtcgccat ctttggttat
     9901 tccaatcgct atatgacagc tttttgagag tactcgattt tcttctcgta cttttatata
     9961 gagtacatcg gtcattaagt aaggataatt tttttctgat aataaacgat tctgccactc
    10021 gttaaccatt ggttctagct gttctgttaa gctagaaacg aaggacttag agacggattt
    10081 accacaaagt tcttccacaa tttttgatac tttacgagtt gaaacgcctg atacatacat
    10141 ttccaacatt gaagcaatga gggctttttc gtttcgttga taacgttcaa acactgtggg
    10201 tgaaaaatgg ccatcacgtg ttctgggtac ttttaattct agcgtgccta tacgtgtcg
//
LOCUS       DAFFSV010000103        10167 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFFSV010000103 DAFFSV010000000
VERSION     DAFFSV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.103"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(46..366)
                     /locus_tag="LR110_002236"
     CDS             complement(46..366)
                     /locus_tag="LR110_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL4823182.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="LR110_002237"
     CDS             complement(514..2748)
                     /locus_tag="LR110_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL4823183.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="LR110_002238"
     CDS             3227..3493
                     /locus_tag="LR110_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBL4823184.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="LR110_002239"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="LR110_002239"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBL4823185.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5384..6430
                     /locus_tag="LR110_002240"
     CDS             5384..6430
                     /locus_tag="LR110_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL4823186.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6447..7670
                     /locus_tag="LR110_002241"
     CDS             6447..7670
                     /locus_tag="LR110_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL4823187.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7759..8646
                     /locus_tag="LR110_002242"
     CDS             7759..8646
                     /locus_tag="LR110_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBL4823188.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8732..8962
                     /locus_tag="LR110_002243"
     CDS             8732..8962
                     /locus_tag="LR110_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBL4823189.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9019..9984)
                     /locus_tag="LR110_002244"
     CDS             complement(9019..9984)
                     /locus_tag="LR110_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL4823190.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3243 a         1789 c         2049 g         3086 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttc
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtagtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 acaacgtatc aaaagctggt gtggcttgta aaagagctac gccagttttt ttgttctttt
     5281 ttaagaaaac gtttttgtca gtaagaatag acaaaaatag ggcttaagtt tgagtgacag
     5341 tttgtaggct atgttatact atataagtga agggggacgt aaggtgaagg tattattata
     5401 ttttgaaagt gaaaagatgc ttgcaaaatc tggaatcggg cgtgcgcttg atcatcaaaa
     5461 acgtgctctg acggaagtcg gtatctctta tacactagat gaaaaagaag attatgacat
     5521 cctgcacatc aatacatacg gaatcaatag tcataatatg gtcaataaag ccagaagaga
     5581 aggaaaaaaa gttgtctatc atgcgcattc tacagaggaa gatttccgaa attcctttat
     5641 cggatcgaac cagctttctc ctatcgtaaa aaaatatctg gtaggattgt accaaaaagc
     5701 ggactatctg atcacgccaa caccttattc aaaacagttg ttggaaagct atggtatccg
     5761 tgtgccgatt caagctattt ccaatgggat cgacttagag aaatatcacc ctgatccaat
     5821 gaaagaacaa aagttcagag aatatttcaa actgtctccg gatcaaaaag taatcatttg
     5881 tgtcggtttg tttttcgaac gtaaaggaat cattgatttc gtagaaattg ctaagaagat
     5941 gcccgaatac acatttattt ggtttggtca tgttcccatg tactcgattc ctcggaacat
     6001 ccggaaaatt gtaaaagaag atcatcctga aaatgtcctg tttcctggtt acattcgtgg
     6061 agagatcata gaaggtgcgt attcaggtgc agatctattc tttttccctt cttatgaaga
     6121 gacagaaggg atcgttgttt tagaagcttt agcaagcaaa caaaacgtac ttgttcgaga
     6181 tattccagtg tataatggct ggttagaaga tagcaagaac tgctatatgg ggaagacaaa
     6241 cgaagaattt atccgactga tccaacagat taccaatcaa gaactaccga gtacgacaca
     6301 agccggttat caaacagcaa aagagcgaag tatcaataaa atcggtgaag aacttaaaaa
     6361 tgtatacgaa agtgtactga atgaaaaagt ctctagcaaa ttaaaaaaag taaatcaggt
     6421 caaggactag tttggaggcg aaaaacatga aaatcggctt ttttaccgat acctactttc
     6481 cgcaagtcag cggagttgct acatcgatca aaacattaaa acaagaatta gaaagacatg
     6541 ggcatgaagt ctatattttt acaacgaccg atcctaatgc ggataagttg gaaaaagatg
     6601 tgattcgaat gccaagtgta ccatttattt ctttcaagga tcgaagagtt gttgttagag
     6661 gcatgtggta tgcttatttg gttgccaaag aactagaact agatcttatt catacccata
     6721 cagaattcgg agcagggata cttgggaaga tggtagctaa gaagttgaaa atacctctga
     6781 tccatactta ccatacgatg tatgaagact accttcatta tatcggtaaa ggaaaagtga
     6841 tcaggcaatc ccatgtaaaa tacttgtcta gattatttgc aaatcatacg acaggtgtag
     6901 tttgtccaag tgaacgtgtt attgatacat tacgcagcta tggtgtaata gcaccgcttc
     6961 gcgtgattcc tactggtatc gacattgaaa aattcaaacg ttctgatatc actcagcagg
     7021 atatcagcaa attaagggag tcactaggtc ttcaggaaaa ccatttgatg attctttctt
     7081 taagcaggat ttcttatgag aaaaatatcc aagccttgat taatgggttt cctcaggtca
     7141 tctccgctta tcctaacgcc agattagtta tcgtaggaaa aggaccttat gtagaagagt
     7201 tagaggaatt gatctcagaa ctgcagttag aagaatttgt gcagttcaca ggagaagtgg
     7261 acaacaatga tgtagcactt tattataaag cggcagatta ttttgtcagt gcttctactt
     7321 cagaaacaca agggcttact tatacagaag cgatggcagc cggaacacct tgtgtcgtgg
     7381 aaggaaatgc ttatttgaac cgattgttcg atcatccttc attagggaaa acttttgaaa
     7441 ccgacgaaga ctttgcacca gcacttattg attatatttc cagtgaagtt cctaaagatc
     7501 caaaagtttt agaagctaaa ctttatgata tttcatctac acatttcggc gaagctatga
     7561 tcgaatttta tcaagacatg gttgtttatc atgaagaaga gatgcgaaga aaagaagaag
     7621 aagactcgat tgaacgaatc aaaataaaat taaattcatt taaaagataa aattcaaaca
     7681 ttttttatga aaaaatcctt gtttctagcg tatactagca tagaagcaag gatttttgat
     7741 ttttaggagg tcgaaaaaat gaaacttggt tttatcggaa caggcgtcat gggaagtgct
     7801 gtcgcacgcc atctactaga agcaggacac gaagtagctg tctataatcg gacaaaagca
     7861 aaagctgatc ctttagtaac agaaggagct atctgggcag atacaccaaa agcggtagca
     7921 gaacaaagta atattctctt tacaatggta ggttacccca aagatgtaga agaaatctac
     7981 tatggacagt cggggatttt ttctgcagat atctcaggac atattttagt tgatttgact
     8041 acaagtacac caagcctggc agaaaaaatt gcaaaaacag caaaagaaaa aggcgcagat
     8101 gcgttagatg caccggtatc cggtggtgac ttaggagcaa agaatggtac actgacgatc
     8161 atggtcggtg gagaagaagc cgtttatgat caagtactgc ctttattcaa agaatttggt
     8221 acgacattta cattgcatgg ttctgctggg aaaggtcagc atacaaaaat ggctaatcag
     8281 atcatgattg caggtacgat gacaggaatg acggaaatgc ttgtctatgc acaaaaaaac
     8341 ggacttgatt taaagaaagt gattgagacg ctttcaggtg gaagtgcagc taactggtct
     8401 ttaagcaatt attctcctcg tattttaaaa gaagattaca cacctggttt ttttgtcaaa
     8461 cattttatca aagatttaaa gattgcatta gaagaagcag aaaaaatgga tcttgtattg
     8521 ccagcaacta cccaagcatt gaaactatat gaagagttgg cagataaagg ttttgagaat
     8581 gacggaaccc aagctttgat caaactttgg tggcctgaag gaaaaatacc agaaaaaaaa
     8641 tcataaaaaa cgagtttttt tagagaaaat caatgcaagt aggctatgct tttgatacaa
     8701 tataacagaa ataagaaaag gaggacctcc gatgacacat tcacaattag tcgcaatcat
     8761 aaaacgactg gaggctatgg tcgagtcagc agataatgaa ctgcaagttc gccgttttga
     8821 aaaagaaggc gtagagaagt gtacagtaac ttatgataaa gcgaccgata catttgaatt
     8881 aactgaaaca gatactcatc agcaatacga atttgataat atcgatatcg tagcgatgga
     8941 aatctatgac ttaatccaat aaattttagg ataattgaca tttgaagttg aagatgccta
     9001 aaagaagccg agaagcaact aatttagttg cttttcagct tcttttttgt ttaacagttc
     9061 aatttcaaac tgttcttttt ctaatttttt gcggtaactg atcaagattt gtacaggatc
     9121 gatttcttct tttgacaatt gacgtttcaa agataaatga gcttctttct tttcataata
     9181 gaagtcagga gaaacgtcac cagaggtaat cattgtttct gcaagttgac cataacaaac
     9241 gaggtagtgc tttttctgac tctcatctgc tgctaatggc atgctgaagg tgagcgatgg
     9301 tgttcttaaa aactgctttt gttcctggat ccgtctaata aaataagtaa ctaattcgat
     9361 atctttttga agcataccgg ctttttcgaa attcaactga tagatgaaat gttgttgtct
     9421 ttttttcaag taagtcagta gctttttatt tcggccttga aacagccctt ggagttcctt
     9481 ttttttctgt tcaaaagtca tttcctgtag gtcaggaagc tggactcgca aagctaacaa
     9541 actaatttca gacaaccaag gcatttgata agtttcttcc attatacgca aaatagacgg
     9601 cattttttta tagagacgaa aaggcccgta tgttcgagcc gtaggagtat ctgttaatac
     9661 aaatccctta ttggtaatat gtacataatt ataattactg aaataattca tttgtcggtt
     9721 ataaaaagga cggtaatgtt ggatcaattg acactcgagc aataaagcat ctaattccgt
     9781 gtctactacg acgaaatcaa aatctgttat atgatgaatc atacgtaata ctttttttga
     9841 atgctgttta ttctgatgaa aatagctgct tactctattt ttgagccgtt ttgctttacc
     9901 aacatagatg accttaccat ctcggtcttt cataagatag acacccggca gcatggggag
     9961 agttttcatt ttttctttta acataggatc tccttttacg ctttgaataa aaagactata
    10021 acaaattttg aaataatgga caagttttag ggaattctat agttctgttc acagaaaata
    10081 ctttgataat caaaatatgt gagatagatg atttttctaa taaaagaaat tataaatgct
    10141 gatttatcag ttgttttaaa tgagtaa
//
LOCUS       DAFFSV010000104         9836 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFFSV010000104 DAFFSV010000000
VERSION     DAFFSV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.104"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="LR110_002245"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="LR110_002245"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HBL4823191.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="LR110_002246"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="LR110_002246"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HBL4823192.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="LR110_002247"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="LR110_002247"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HBL4823193.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="LR110_002248"
     CDS             complement(4028..5044)
                     /locus_tag="LR110_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4823194.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5343)
                     /locus_tag="LR110_002249"
     CDS             complement(5146..5343)
                     /locus_tag="LR110_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823195.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="LR110_002250"
                     /pseudo
     CDS             complement(5315..6634)
                     /locus_tag="LR110_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7375..8355)
                     /locus_tag="LR110_002251"
     CDS             complement(7375..8355)
                     /locus_tag="LR110_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBL4823196.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8368..9246)
                     /locus_tag="LR110_002252"
     CDS             complement(8368..9246)
                     /locus_tag="LR110_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBL4823197.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9262..>9836)
                     /locus_tag="LR110_002253"
     CDS             complement(9262..>9836)
                     /locus_tag="LR110_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBL4823198.1"
                     /translation="FLEKLEKSVKNDLSFLTKNLGMIKEKDEEEMLWKVDGIEYVLDK
                     VEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFVLILFSSTHEYDLPLEKYY
                     IHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDVTWTKNHAMVNLAKEYVKH
                     DAYTLKVIAESPEELKAKYGEGFEGIRKIY"
BASE COUNT         2649 a         2301 c         1678 g         3208 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac actctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcattt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
     6781 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg
     6841 gtaattttta ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta
     6901 atttcatttc tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata
     6961 tttttcaact tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact
     7021 aacttcttta acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat
     7081 ttcatcaaat gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa
     7141 ttttagtata acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc
     7201 cacccaaaag ttagattttt tggtctaagt tttagggtcg cttcattact agaattcaac
     7261 aagagatgtt ttttatttta tttaaattat ggggtcagtt tccatttgat tacaaacttc
     7321 tttgttttct ttttttaaac gcaataataa aggttttaat ttcagccttt ttaattagaa
     7381 aatcaatttt cccaaattat tttctttagc tttcttaacg atttctgtcg caactaccac
     7441 atctaataca gccgaaccaa ctgttttgaa gatcgtgatt tgttctccac ttgtccgacc
     7501 gatcaattct tcattgatca gttggcctag ttccccatga tagctgtctc tttgaatata
     7561 cccatcttgc agcggtgaaa tgaagtcccc agcttctttt agaacaccgt ccatggtatc
     7621 aaaaataaca acatctgcgg ctttgattat ttctcgtgga atttcacaca tttccggtgt
     7681 gtaagcaccg actccattga tgtgcgcacc ttttttgacc cactcagcag agaatgtcgc
     7741 acgttttgaa gtggtcacac ttgtaatgat gtctgctcct tcaacgcatt cttgactggt
     7801 ttttgttggg cgcatcgtta cagaaaattg ttgcatcatt ttttcagcaa attggctggc
     7861 acgctcaaaa tcaatatcaa aaatccgaac ttcttctaat ttacgaacgg tcaacattgc
     7921 ttctaattgt gactgtgctt gtccgccggt tccaatcagc gcgccaattt tagcatcttc
     7981 tcttgcaaga agttctgttg ctgctccttg aacagcgcct gttcttaatt gtgtcaaata
     8041 agtaccatcc aaacaagcgg ataccatacc cgtttctgga tccaatacga tcatcgttgc
     8101 tggtacactt ggtaagtttt tcttgatgtt ttcaggatac acggaaacga ttttgacacc
     8161 tagcgcgcct tcgccacctt ctacataagc aggcatatat aagctttgtc cgttgctttt
     8221 tggcacatcg atattggtgc gaagaggcac acttgccttt cctgctgaat acaatgacag
     8281 tgcttttttg tccgcatcga tggcttctct cattgaaaaa cttttgataa tatcttcttt
     8341 tttaagaaat aacatttgaa tcgctcctta attagctgct gtaacttttt ttaattgact
     8401 gcccaacatc gtatatgcac tgtatagtaa gaccaaaatc acgatccatt tcaggatata
     8461 ggtatctaaa gatttgacta ggaatacagc gaccaatacc cccaatacac cgaaggttga
     8521 agtaaaaagt gtgatttttc tgctgtattc attaaattta atgaattgaa cactgccgat
     8581 cggtactgag aaggtacaag cccccatcat aataggaaag gctgcaactg gattcaagcc
     8641 tagcaagtag acagttacca tcgttaacgc ataagaacca atgccgatat tgttcaatgc
     8701 accatagaca aagagtaaaa atcctgccaa gattaatttt ccgccataca attcactggc
     8761 tgttccgttt gatggaatcc agttcatttg tcctgcaaaa atcaacaatg cagcaatgac
     8821 caatcctatg ccgacaaaca gtttgatcgt tttctctggt aacttcacga caaatcttgg
     8881 tcctaaatat gcaccgataa tttggcagat aatacaaaca gctagtgtct tgatccctac
     8941 gttgatggca gtgatgtaag acaatgccat gacggctacc ggtactacac attgggtatt
     9001 caacgttccg ggtagttttt tcatgctgac ccatttcaac tttggataaa ggacggttcc
     9061 gatagcaaag tcggaaacgc caaatgttga aagaaagaag atgataaatg acgtgaaggg
     9121 caacagacgc atatcccctg gttcttcttt gaaattttgt ttgtttttga aaaggtctct
     9181 tagaaagtaa ataataaaat atagattgat tgcgacaatt agtgtcagca aaatattccg
     9241 aatcataaaa aattcctcct actagtaaat ctttctgatt ccctcaaagc cctcaccata
     9301 ttttgcttta agttcttcag gagattctgc aatcactttt agtgtatatg catcatgttt
     9361 gacatattct tttgctagat tgaccatggc gtggtttttt gtccaagtga catcggttga
     9421 ttgcagttct tcgttaatgg taccaaacac gacttcttgc tcatcggcga cgatgttgat
     9481 ccatcttgct ccaaagtcag caagtttgtc tgtttcaaaa ccatgaatat aatacttttc
     9541 taagggaaga tcatattcat gggtagaact gaataaaatc agtacaaact tatcaactct
     9601 tttttcagct tgctgcaatg cctttagtag tgaatccgtg aaattctcat gccaaacttg
     9661 aatgagcaaa ctttcttttg cgtttttcac caaatgctct actttatcca gaacatactc
     9721 gataccatca actttccaca gcatttcttc ttcatctttt tctttgatca tccctagatt
     9781 cttggtaaga aaagaaagat catttttaac acttttttct aatttctcta aaaact
//
LOCUS       DAFFSV010000105         9833 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFFSV010000105 DAFFSV010000000
VERSION     DAFFSV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.105"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            278..787
                     /locus_tag="LR110_002254"
     CDS             278..787
                     /locus_tag="LR110_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4823199.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="LR110_002255"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="LR110_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL4823200.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="LR110_002256"
     CDS             2322..3302
                     /locus_tag="LR110_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4823201.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="LR110_002257"
     CDS             3424..4842
                     /locus_tag="LR110_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBL4823202.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="LR110_002258"
     CDS             4913..6382
                     /locus_tag="LR110_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4823203.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="LR110_002259"
     CDS             6392..6859
                     /locus_tag="LR110_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL4823204.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="LR110_002260"
     CDS             6895..7767
                     /locus_tag="LR110_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL4823205.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..>9833
                     /locus_tag="LR110_002261"
     CDS             7849..>9833
                     /locus_tag="LR110_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL4823206.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFD"
BASE COUNT         3400 a         1429 c         1966 g         3038 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttt
//
LOCUS       DAFFSV010000106         9798 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFFSV010000106 DAFFSV010000000
VERSION     DAFFSV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.106"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..815
                     /locus_tag="LR110_002262"
     CDS             <1..815
                     /locus_tag="LR110_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBL4823207.1"
                     /translation="LLSGCSKWIDRGESITAVGSSALQPLVETVAETYQTENPGKFIN
                     VQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRVAVVGITPIVNKDIGV
                     KDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRSTFEKWVLDGETAVQAQ
                     EQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTDENVTTNKWTIWAYEH
                     MYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVERDWQGNIVKE"
     gene            930..1850
                     /gene="pstC"
                     /locus_tag="LR110_002263"
     CDS             930..1850
                     /gene="pstC"
                     /locus_tag="LR110_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBL4823208.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1850..2734
                     /gene="pstA"
                     /locus_tag="LR110_002264"
     CDS             1850..2734
                     /gene="pstA"
                     /locus_tag="LR110_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBL4823209.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2749..3555
                     /gene="pstB"
                     /locus_tag="LR110_002265"
     CDS             2749..3555
                     /gene="pstB"
                     /locus_tag="LR110_002265"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL4823210.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3571..4329
                     /locus_tag="LR110_002266"
     CDS             3571..4329
                     /locus_tag="LR110_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL4823211.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4342..5019
                     /gene="phoU"
                     /locus_tag="LR110_002267"
     CDS             4342..5019
                     /gene="phoU"
                     /locus_tag="LR110_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBL4823212.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5242..6273
                     /locus_tag="LR110_002268"
     CDS             5242..6273
                     /locus_tag="LR110_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL4823213.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6270..7037
                     /locus_tag="LR110_002269"
     CDS             6270..7037
                     /locus_tag="LR110_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL4823214.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            7137..8705
                     /gene="liaX"
                     /locus_tag="LR110_002270"
     CDS             7137..8705
                     /gene="liaX"
                     /locus_tag="LR110_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBL4823215.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            8881..9189
                     /locus_tag="LR110_002271"
     CDS             8881..9189
                     /locus_tag="LR110_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBL4823216.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            9189..9539
                     /locus_tag="LR110_002272"
     CDS             9189..9539
                     /locus_tag="LR110_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBL4823217.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            9677..>9798
                     /locus_tag="LR110_002273"
     CDS             9677..>9798
                     /locus_tag="LR110_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823218.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNV"
BASE COUNT         3159 a         1688 c         2105 g         2846 t
ORIGIN      
        1 ttcttttatc aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct
       61 cttctgcttt acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg
      121 gaaaattcat caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag
      181 gagcagtcga catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct
      241 cagcattagt cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata
      301 ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa
      361 actggagtga agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa
      421 gcggaacccg tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac
      481 aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca
      541 gctatgttgc tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag
      601 aaccgacaga tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt
      661 acacaagaga tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg
      721 aagtacaaga taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg
      781 aacgtgattg gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta
      841 gctaaacttt tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta
      901 tgttaaacct gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa
      961 aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc
     1021 tgatcgtgtt ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct
     1081 tttttactga taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc
     1141 aaatcggagc aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta
     1201 ttgtgacctt tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca
     1261 tgacggagat ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg
     1321 taggtattcc ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca
     1381 gaagtatttt tggcgggaca gggtttggta tcttagctgg tacatttgtg ttgttcgtta
     1441 tgatcttgcc gactgtcaca acgatgactg ttgatgcatt aaaggcagta ccacggcact
     1501 atcgggaagc ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc
     1561 tacgagcagc cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg
     1621 gtgaagcctt agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggactta
     1681 ctactcctgc ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg
     1741 gaacagttga aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat
     1801 tctttaatat cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa
     1861 acgctcagat aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat
     1921 tttagcagct ttactacttt atatcttagt tcggggaata ccgcacattt catggtcctt
     1981 cttgaccgaa ccttccaaag cctaccaaga aggtggtgga atcgggatcc agctatttaa
     2041 ctcgatttat ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg
     2101 tatctatctt tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat
     2161 tgaaatcttg agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt
     2221 tattcaattc cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa
     2281 cttgcctctt ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg
     2341 agaagcagga ttggccttag gtttgtcacg ctgggaaaca gtgatgaaag tggtggttcc
     2401 agaagcgaca ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga
     2461 agcagctgca ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg
     2521 gaatccatta agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt
     2581 ccatatttgg aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg
     2641 cgcttctgcc gtgttgatca ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg
     2701 caaacgatta taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat
     2761 aactggaatg aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg
     2821 gatgatatac atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt
     2881 gaaaaaaata agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt
     2941 cgttcgttga atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg
     3001 tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt
     3061 ggcatggttt ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt
     3121 gctttgaaac agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc
     3181 ttgaaacaag ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct
     3241 ttatctggcg ggcaacagca acgtttatgc attgctcgtg cgatcgcgat gaaacctgat
     3301 atcttgctgc tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa
     3361 gaaacacttg taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa
     3421 caagcagctc gtatcagcga ctatactgcc tttttctatc taggaaaagt aatggaatat
     3481 gacaaaacta acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc
     3541 ggtcattttg gttaagacgg aggcctcaac atgacaaaag aaattatttc atctaaagat
     3601 cttcatctgt attatggaaa gaaagaagca ctaaaaggga tcgatctaag tttcaatcaa
     3661 ggggaaatca ctgcgatgat cggtccttct ggttgcggaa aatccactta tctgcgttct
     3721 ttgaaccgga tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa
     3781 gacaaagaca tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg
     3841 gtattccagc agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg
     3901 aaattaaaag gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag
     3961 gcagcgtctg tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc
     4021 ggcgggcagc agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc
     4081 ttattggatg aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg
     4141 ctgttagaat taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct
     4201 tcacgtattt cagataagac ggcattcttt ttagatggtc acttgatcga atacaacgat
     4261 acgaagaaga tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga
     4321 tttggataag gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc
     4381 cataatcagt tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt
     4441 gcatacatca aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc
     4501 aataacatgg aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca
     4561 gttacaactg atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt
     4621 atggccgatc atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga
     4681 ataccggaaa tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac
     4741 aacgtcctag ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg
     4801 gatcaaagag tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag
     4861 gaaaatcctg agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa
     4921 cggatcggag actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa
     4981 atcactgagt taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg
     5041 tttgtttatg aaaagaggtt gtgaaaaagc tgttctacat caagtaataa gaacagtcaa
     5101 acaaaaaata gctcctcata tttttcgttt ggctggactt attaccgcag atgcaagctt
     5161 ttgaacaccg tttattcggg aaagggtttg tgacaaaaca tgtcacaaac ccttttcatc
     5221 atttcataga aaggataaag aatgcaaaaa aaaattagca tcatcactat attttttatt
     5281 tgtgtgctta cttcatgggt tattcagcat caagccgctt tatatgaagt acctgtcatc
     5341 gagatagaaa aagtacgaat aaaagaggat catcaattga ttacaggaaa actgatgaac
     5401 tcggatcaaa aaggagaatc gatttattta tcggttcctt atcaaaaaaa tcaaatagaa
     5461 acagcaaata taaaagcgag acaaatgtat ctagtccaaa tgcatggaga tgaatggcag
     5521 ctagtagcaa aaaaaaatga tggctggctt ttcttcttca caagtatctt cattggcgcc
     5581 atgttgctta ttggcggaaa acagggtcta tttgctttag gaagtctcat catcaatatc
     5641 gttttacttt tacttttgtt gctcatcttt actaaaacag aagggatttc tctattaaat
     5701 aaatctatcc tgttcgtcgt tatagggatg attattagca tattggcttt agatggatgg
     5761 aatagaagca gtataatcaa aatttcagct gcagttacga gtgttttttt agcatttttt
     5821 atttgtcttt tgacaatgaa tcattggaaa gaccaaggac tgcgctatga ggaactgaat
     5881 tatctgacac gaccgtatcg ttctgttttt ctttctagcg tactgattgg aacagctgga
     5941 ggaacactcg ataccgttat cacagtcatt gcgacattag aagaattgac taagcgacaa
     6001 ccagaaataa cagcgaaaca gttatggctt tcaggaaggc aagtgggtcg agatgtgatt
     6061 ggctcgatga ccaatatcct tttgttcgtc tatatcagtg ggtcagttcc atcgcttctt
     6121 ttatatttag gaaatcaatg gtctttcaaa gaaacgatag aacagcattt atcattggaa
     6181 tttttacgcg tcattgctgg cagcttagga atagtattat cgatccccat ctcggtcctc
     6241 ttttttctga ctgtaaggag gctgaaacga tgaatgtact tattatactt gttgggattt
     6301 tagcaatcag cgtgctactt attggcggta cgcaaggtat gtatattctt ttaggcttat
     6361 tcatcaattt agggatcttt ttcctgttgt tattcggtta tcatcagaag tggccgatac
     6421 tggcattgtc ggtcatagga tttttattga ttgctgtggt tatcttattt tttatcaacg
     6481 gttataatct aaaaatgcgt gcagcttttg tgagtatact gatcttttta ttttgtttcc
     6541 tactattgct tcctatcact gattttttag cgatccaagg ttttacatcg gtagaactag
     6601 aagaattatc tggcttagat aagactttag cgattgattt tagattactt acgagatcgt
     6661 tgcttctgat tagtcttagt ggtgctgttt tagatgcgag tgtagcaatt tctagtggga
     6721 cattcgaagt atatcaggca aatccgcaat tatcttttta ccaattgcgc cattctagtt
     6781 tcgctattgc taaaaaaata ttggcgtcaa cagtaatgac acttcttttt gcattcatgg
     6841 gaagttcttt agcgttgatc ctctggttta ttgatttaga cattcctttt cagcaagtga
     6901 tcaatgaaaa atcatttgtt ctagaatata cgatggctat tcttaccaca ttgtccgcac
     6961 taattgttct tcctttaacc tgcgtgacta cagcatatct ctttacaaaa aagaaagaac
     7021 aactcaagat ggagtaaaag ttacaactta agtcctatga catttttact gaatcagtgc
     7081 ataatatgga tgtaaggaaa agcttgcaaa caattttgct taaggaggca atttccatga
     7141 aagaaagaga acgtatttta gatttagtga aaaaaggaat cctttcgact gaagaaggct
     7201 tagatttatt agaaagcatg gcaactgaaa aagacgaaaa gcaaatcaaa aaagaagcag
     7261 acaaggtaac tgctagtcat aaagaaaaag ataaggcaag tcaattgatc gataaacttg
     7321 aaaacggcga agaagaaatt tctgagccag ttgatccaaa ggaaagagaa agacaagatc
     7381 aagaaaactt agaaaagatc ttagatgaac tagcaactga agcgaataaa acttcggcgc
     7441 gtctagatga agtaaatgct caattcgctg acaataaggc agcacgcaat gagaaacagg
     7501 aagcattgat gcagctgaat acaaaagaag aattaggcga gttgacagaa gaagaattag
     7561 cacagcgtca ggcgttagag gcagaaatca aagagttaga agctagtggc gatacactgt
     7621 tagaagaaca attgaaatta gaagcagaat taaaagatat ccgtaagaat caatggtctg
     7681 agaaaaaaga aacgtttact gataagtttg aattgccaga tgattggaaa gatcaagcaa
     7741 cagatacact gaatcaagta ggcgaaaaaa tgtcagaagc tggatcacag ttaggcaaat
     7801 tcttgaagaa aacattccaa actgtttcag aaacagtgaa tgacaacatg gaatggaaag
     7861 atgtcagctt acgtgtccct gggattgcga caacgaaatt tgagcatgaa ttttactatg
     7921 aagctcctgc agcaagtatc cttgatatca aggcagcaaa cggaaacgtg acgttaaaaa
     7981 cgtgggacag caacgatgtg aaagttgaag ctaagatcaa actttacgga aaaatgggtg
     8041 ctgaaccatt tgaagcattc tctgaacgga gccagattga ggttaatgaa gatcatatct
     8101 ccttccaaat tccaaataaa cgtgtgcgtg cagaccttgt tttctactta cctaaacgag
     8161 tatacgacca cgcagctatt aaacttctaa atggcaacat catgattgaa acattggaag
     8221 ctaaagatat ttatacaaaa tctacgaatg gtaacatcat cgtggaccaa ttaactgcaa
     8281 cgatgctgga agtagaaggt gtaaacggca atatcgatat tcgaaacggg aacatcttag
     8341 attctattat cgaaacagta aatggtacag tgacatttgg tgcgacagca gaaaatcttt
     8401 ctgtgtcttt agtaaatggt gatgtacgtc ttacgattaa agaagataat ttgaaaaaag
     8461 tagaagcaag ttcagtgaac ggaaatgtca aagtcgcttt accagatggc atcggtttgg
     8521 aaggacatgc taaaacaagt cttggaagta tcaatagccg tctgtccaat tacgaagtag
     8581 ttcgtgagaa aaaagaacgg acaaaccaaa tgttgcaatt ccgtcgtgtt tcagatgatg
     8641 agatcgcgca agtgcaactt tcaacgacaa caggaagtat ctatttaaaa gatacagata
     8701 aataaagagg ttgtgaaaga gatgttcagc cttgagcaat aagaagcgat tttgtaaaat
     8761 agcttcccct attttacaaa attgtgcctt attgccgata ggctatctct tgaacaccga
     8821 ttaagaagaa ggttgtcaca acgattttct tcatttaaaa tagtagaaca gaggtgagac
     8881 atgatgaaaa aaaagttgac aaaatccaat aaaaatgttg ttttgacagg tactttagca
     8941 gggattgggg aatatatcgg gattgatccc actgttatcc gtgtgcttta tgtattccta
     9001 agcttagttg cttttggaag tccaatcatt ttatacattt tattagcttt gattatccca
     9061 tctgcgcgtt ctgcacgaac aaactatgga cataacaatg catattatga aaaaaacaat
     9121 tatcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa tcgatgatga cgaatggagt
     9181 gacttttaat gtcgtacttt cagcgtattt tggttaatac gttaacattc atatcgttgg
     9241 ctgttctttt tcctgcgatg gtccatgtga gcagtatttg gacagccgtc cttgccagct
     9301 tcgtattatc tgtgttgaac atgtttgtca aaccgattct gacgatcctg tccctgccct
     9361 ttacattagt cacattggga ttgtttacgt ttgttatcaa tgcagcgatt cttaaaatga
     9421 cttccttttt tgtaggagca gaacatttta gtttttcttc atttggtgca gcgttattcg
     9481 tagctatctt gatgtcactt gtcaatgtaa tcgttagtga acataacagc agaaagtaaa
     9541 aaaagctgtg ccgagatctc ggcgcagctt ttttgtttat cgcgaattgt aaaattaagt
     9601 tagaaaaata aaaaggcatt tatggtacac tcaaattgta tttccgacga aagaaaacaa
     9661 aggagtgtaa ccataaatga cctacaaaca tcttaccata gacgaactga caatgataga
     9721 atcatattat cttcaacata ataagccggt tgaaatcgct aaccgaatgg gacgtgctat
     9781 acaaactatt tataatgt
//
LOCUS       DAFFSV010000107         9745 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFFSV010000107 DAFFSV010000000
VERSION     DAFFSV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.107"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            461..1696
                     /locus_tag="LR110_002274"
     CDS             461..1696
                     /locus_tag="LR110_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL4823219.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2695..2955)
                     /locus_tag="LR110_002275"
     CDS             complement(2695..2955)
                     /locus_tag="LR110_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL4823220.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2982..4283)
                     /locus_tag="LR110_002276"
     CDS             complement(2982..4283)
                     /locus_tag="LR110_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4823221.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4575..5033)
                     /locus_tag="LR110_002277"
     CDS             complement(4575..5033)
                     /locus_tag="LR110_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL4823222.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(5046..6377)
                     /locus_tag="LR110_002278"
     CDS             complement(5046..6377)
                     /locus_tag="LR110_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBL4823223.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6388..7830)
                     /locus_tag="LR110_002279"
     CDS             complement(6388..7830)
                     /locus_tag="LR110_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4823224.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(7817..8581)
                     /locus_tag="LR110_002280"
     CDS             complement(7817..8581)
                     /locus_tag="LR110_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBL4823225.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8685..9560)
                     /locus_tag="LR110_002281"
     CDS             complement(8685..9560)
                     /locus_tag="LR110_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL4823226.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         3155 a         2014 c         1466 g         3110 t
ORIGIN      
        1 ataatattca aattaaacaa ctggcgtatc tgctccttga tttctttttg tgttatctga
       61 ataattatcg tttacagtta taacacatag tgaaaattgt catggcgaga aagtctaaga
      121 atatttttaa acataactat ttctttctta cgtgtgtata atgacgacag cacacctatc
      181 aattaatata attattttat ttttttatgt tctcatgttt atttttaatg acacaacttc
      241 tcgtatttat ctggcacaac atataaatcg atcaaaaaat tgttgcgtgt ttttttgaaa
      301 attggagttt caacttgatt acaaaattta aatttagaag ttgtgaaaac agcgagagac
      361 agtttaatat tcaaaatata cataggctac tcgaacgctg tgttttttag tatactagta
      421 agaaacgaag ggatagactc attccaataa ggaggatttc atgaagacaa ttctttttgc
      481 tcctgcagta ttcaatttag ctgagacaac acgaatgatt gaggttgcca aagctatgaa
      541 agaccgttat tactgtgtat tctttggctt ttctaaaact tttgctcctt tgatcacaga
      601 aagtggcttt gagtatcatt tactagcgcc gatccttacg aaaaaacaag aagaacaaat
      661 cataaaattt gaccagttgc gtacacttaa aaaccctttt accaaagaaa tggttgcctc
      721 tcgtatcact agtgaacaga gtctgattgc taagtttgag cctcgactga ttgtgacagg
      781 ttcgaatgtg acaatttttt tatcggcccg caataaaaag attcctttag tatatgttaa
      841 accctatgct ttgagtgaac ctttttttca atcagagcat gcgctgatgc cccacgagtt
      901 acgtttcttc ggccctttaa gcaatcccct ttggcactgg acaaaaaagc gtatagtaaa
      961 tacccgatgg atccccaatg ccttcaaaca cgctgcacaa aaagaaatgc ttcctttacc
     1021 aaaaacaagt ttacaaatga tggatgctga tctaaatctc atcacaacgc ctaaactatt
     1081 tttggaagaa gaacagctgc cggaaaatta tcgaatcgtt ggtccaattt ttgctaagct
     1141 cagtacttcc ttgcctgaag aagtgctaac ttttatacag cgaaagcaac aagaaagaaa
     1201 acgtattatc tatttcgcaa tgggtagttc aggcaatccg cgtatgttac gcaaaatttt
     1261 gttcttctta caaaaacaac cggatctagc ggttattgca cctattgact atctgcttga
     1321 tgacattcaa tctgactcag aagatctttt aataacagaa tatctgtcta gtcatctttt
     1381 gcatgaattc attgattttt cttttatcca cggcggggaa gggaccgtcc aaactgcctg
     1441 tgaatccggc aaaccgttta tcggtgtggg gatgcaattt gagcaaagct gtaatattga
     1501 ctattgtgta aaatacggaa acgcaatcgc cctcacaaaa ccagtgactg aaagaaaact
     1561 gcagattgct ctgcgcgaag tttcttcacc caagattcgt cagcaggcac gcacgctgca
     1621 agccgttttt ccaataaatg gtccacaaca agcaattcaa gaaatcgaaa ccttcctgag
     1681 caaccaccca atatagtttc aagtagaaaa agctttttta atagagactc atcagtaatt
     1741 tcgctattcg agacccagac agtgaaaata caaaaaaagt aaatagtgga ttgaaaagtt
     1801 agagttactt aaattacggt gtctaaaaaa cacaaaatca tccttaaaat aaaagatctg
     1861 acgctatcca aatgagcata ggtagcgtct gccatttatt cttagtcaaa ttctttgaca
     1921 cctttctctg gttggatcat ctcaacgttt cttcaaaaga aagaccataa taattaggca
     1981 aatacatttt cttaaaatgt gtcatataga tctagaagca tcgctttgta gagatttgtg
     2041 ccgctagtcc cctcaacaat tactaaatca aatgtaggat ttaccacttt atctttgata
     2101 atcaacctcc ctcttctgtt gagacaatga tctcgcaatc cgatgcatac tcctatataa
     2161 actgccagca atccccacat attgagtaga tcatgtcaga atgttctatg tcgtcagcaa
     2221 cgatacacta aaagtcacgt tcaccatgac tgattgctat tcccagcaca attatttcta
     2281 tacacgtttc atgatttgaa taaacactca catcatacat attaccattc atacaccata
     2341 ctgcagcccc tactatatgg ttgtaagctc ccaaatctat atttgacttg atcactttgg
     2401 tagtagatct actaattcta aatccattgc tgataaaatt tccatttttt cctccttctt
     2461 tctgccaaaa cagttaaaac aataataatc tttatgtaaa aaagggctgt gacaaaagcc
     2521 ttgtcacagt cccatattcc gaataaacgg tggtcaaaag cttgcatctg cggtaataag
     2581 ttcaaccaaa cgaaaaataa gccaacattt cacaaaaatt taagaagcaa ttttcgtaaa
     2641 tttcttctta tttcttgaag ctaaccactt ctgttacaac cacttttaga acatttaaag
     2701 cttcgatgtg acaaaccatc tatgctgctc gatcataaaa ttgatagcaa aaattccaca
     2761 aagtacatat gtcccccatg aaaacaaaac attcatatgc ggtacaatga tctttggaag
     2821 aatcacgatc aaaaatgatg ccagtgccca aatccatcga ttgatttgaa tatgatatgt
     2881 agctagcata ttcaatataa ggtgactagc cgttaataaa aagataaaaa caaaaaataa
     2941 aatcagccaa tacattgttt cccaccttct aaaaaccaaa cttagacagt ctggacttcg
     3001 ttttctccac gactttgttc atctgacagc atctttttat cataagcttt tacaaatgga
     3061 taatagataa taaatgctat caatgtgttg atcatgatca agataacaac acgccagtca
     3121 tttcctgctg aaagataacc gccgattgga gctggtaatg tccatgttgg caaaacagta
     3181 aatctattga ccatttctaa tttgactgca aaataactga taatagtcaa aactaaagga
     3241 actaagttga acgggatgaa catgtaagga ttcaacatca taggagctcc aaaaattatg
     3301 ggctcattga tattgaagat accaggaata atagaaaaac gaccaacctc tttcaagaat
     3361 tttgatttac agaagaatat aaacaaaaca gataccacga tcgttgctcc tgcaccacca
     3421 attgttaccg tccattgata aaactgttca ggagcgatat agggtaattg ggtggcagct
     3481 gtaccatctg ccaatgcctt tgcattctca tccaacatga tcagccacat tggacgaaca
     3541 acagccccta caatactcat gccatggata ccaaatgacc acaacaaatg gatcaaaaac
     3601 attggtagta aaacaccaag caaattattc cctaaaatat tggttaaagg ctcgaataac
     3661 gataataagg cagcgtttac atcaaagccc gcgacgcttc gaatcagcca agtaattgtt
     3721 aaaatgactg cacctggaat caatgcctca aaactacggg caacagcagg tggcacttgt
     3781 tctggcattt tgatagtaat atttcgattt ttaaagaaac gatatacttc cactgcaaaa
     3841 atcatagata agatccccgc aaacatccca gatgctccca aagaactcat aggtaagacc
     3901 cagccaagag gtgcctcttc aggagttaaa acgtttacag gaacttgtaa cataaagaac
     3961 gttcctaaag ataatatacc acctgtcaca ctatctaatc catatgattt agctagatcc
     4021 gaacccataa caaaagttgc ataaatagcc atcaatccca tcgtcatcct aaagggtaaa
     4081 acaatcgttg ccgaataagg ggcaataaat tcattccatc caggaaacgg taaattgata
     4141 aagaccataa aaaatgtccc aatcaaaatc aatgacaatg ttgaaacaac cccattacga
     4201 attgctaata aatgacgctg tgtaccaatt tttgccattg gtgggacaag tttctcttcg
     4261 acccatttca tcagcttttc cactaaaacc actccttttc aataaaatat gaaccattca
     4321 ttgcatgaca acgcaaagaa tgttcataac taattatata attaagaaat tttatttcaa
     4381 tatattctct taaataatca aatttatttt taagaatcct gaaaataagt tttctaaatc
     4441 ataggagcaa caaaaaaatt cgataattaa caaaaatcaa aatgattaaa tctagtgatt
     4501 gaaacatatc atttcaaaaa aattttgtaa attcacccat aaatatcacc ctatttaact
     4561 tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa tctttacttc ttggttttgt
     4621 tttaataaat ttccttcaaa aatgcctgga gaaactacaa tgatcaccgt atctttttgt
     4681 gcttgctcca acttcttcaa atctataata gctaacagtg tcccttcttt tacctcctgg
     4741 ttactttcaa cccgtatctg aaacggactt ccttcgagtt caactgtatc taaacccata
     4801 tggatcagta tttttctgcc agattcactt tcgatcgtta tggcatgtcg ggtaggaaat
     4861 atattatcga ttattccttt aactggcgca tggatatatc catcatgatt cgttatagcg
     4921 tatccaactc ctatcatttt tgaagaaaag acctcgtctt ttactgctgt taatggaata
     4981 atttgaccac tagctacagc ttttagaatg tttcttgttt taaagaaatg cactttttat
     5041 cacctctact ttaatgctgg ccaatatttt ttgtttactt tgatcatttc atctagtatt
     5101 ttttttgcga ctctggcatt tggtacggtt tgattcaatg taaatgcttg taaagctttt
     5161 tgataagaac cttcaaagta agcatctact agtaacttct ctactgcaac ttgtgcctcc
     5221 atcaatcctt tatgaaaatc actgattggt tttcttagtg taattggctc cacaccagtt
     5281 gcaccaacat atgccggtat ttcaacaact gcatctgacc gtagattcgg aatcgcccct
     5341 tgattaggaa cgattagcat aaagcgacga cgatcatcat tcaacaagga aatagctata
     5401 tcgacgatat attgtccatg ctccccaaaa ttaaagttca atacttcttc cacaataccg
     5461 cggcgtattt tatcagccat ttctttggta tttttctctc gtccatccat gaccatatta
     5521 gcacgtgtat actctttgtc agtatgctca acgaccatat caggatataa gtaatactct
     5581 aagtaattat tcgggatatt acttggaaaa tttttcgtga tgactgacat catttgaaag
     5641 gttttttgcc aagttttatc tcctatatta aaatcggcga cttgcatttc ttttgttatt
     5701 agtttttcaa taatctctgg cataatttct cgttgcaatt ctttatcata aatcgatgta
     5761 taccagccaa agtggtttaa accataatac gtaacgatcc aattcttacg atcataaccg
     5821 taattcactg caatcgtttc ttcaattgaa atagtcatat cacaggcatt aatcattttt
     5881 acattcggat atttacgacg aacagcttca gaaacgatcg actctggatt tgtataattc
     5941 aaaatccatg ctttgggggc atagtcttgt atatgatcga caatttccaa taaacctttg
     6001 attgagcgca tcccataagc aaatcctccg agtccacacg tttcttgtcc tactaatcca
     6061 tgtttaagtg gaatcttttc gtccttttct cgcatctcca atcctccaac acgaatttgt
     6121 gagaaaacaa aatcgcatcc tgtaaaagca agctttggat catccgttgc acgaatgcga
     6181 atatttgaaa aaccttttct tttcagcata aatttaacaa tcaagtacat atccatattt
     6241 ctttcttcat caatatcata taaacgtatt tcagataatg gcaaccgttt ttcattgttc
     6301 aaaatggctt gaacgattcc tggtgtatac gtactcccgc ctccagctat agtgataatc
     6361 tgttttttca tcatcatctt ttcctttcta ttcttctaat aatttattga attctgccat
     6421 aacataagtc acttctaagc cgatgataat ttgataattt ttttcagttg gtttaatgat
     6481 cccgcttatc cctaattgct tgattcgctg ttcctcgacc atggtgggat ccactacctc
     6541 tacccgtagg cgtgtattac aactatagac atccaggata ttggcttttc ctccaaacgc
     6601 ttgtagcatt tttgttgcca tatattcata atttttactt gctaacccta actctgtttc
     6661 ttctaaggtt acttcattgc caaactctgc cttagcttca cgtcctaatg tctgaatgtt
     6721 tctcttattg atcaaataat aaaaagtaac gtaatacaag gcaaaaaaga aaattccgat
     6781 tggaagaatc aaaatagcat tggtagcgat tcgaaaattg atgagataat caacaataca
     6841 tgctccgaat gtaaaaccaa ttcgtatatt gaataagtaa cagacaatcc ctgctaaacc
     6901 ggcaaaaagt gcatggatca cgtacaacat aggagaaaga aacatgaaac taaattcaac
     6961 aggctcagtg atatttgcaa taaatgaagt tcctgcaccg cttcccatga ttcctttgac
     7021 catctcttta ttttctttat aagctgcttt atagatagct agacatatag ctggtatacc
     7081 aaacatcatt actacaaaaa aaactgataa aaacagtcca gcctgaggat ctccgttgat
     7141 aaagcgtgtc atctctcctc tgaaaacttc cttgctagga gatgtatagc ttcctaaatc
     7201 gtaataaata tacgtattca acacatggtg cagtccaaag gggatcagca aacgattcaa
     7261 aaacataaat atccccacac ctaatgctcc catatcaacg agcagttttg caaaattatc
     7321 aatcccaatt tggatgagcg gccagataaa gcccaaaaaa acagctgcga ttgtttgtag
     7381 aatcatcacc atagtcaatg ggaatttttc gcccgcaaaa taagaaaaaa ctactggtaa
     7441 tttttgttct ttaaagcgat tatacgtcca tgccgctact aaaccggaaa taattccacc
     7501 aaaaatcccc atattcactt ctgggtccat aatttctaag ccttttttca aggtagcaat
     7561 cgctaaaaaa ccagtaagtg caggtattcc tttgtcctta tttttgacaa aaccgattgc
     7621 aactcctata gcaaataaga tatccaagtt tccaaaaacg gcatttccag ctgcaccaat
     7681 tactgggatg ttcagcatat cgtcagctga taaacgcatg atcaatcccg ctaatggcat
     7741 cgctgcaact ggaagtagca tcgctcttcc aagcttctgc acatttgatt taacattcat
     7801 ttttattcct cctctattaa atatcctcat atgttactaa ctcaatcaaa ggatcatttt
     7861 tcactttttg agcaaatgct gaatgaatta aaaattcgac ttggtgggta cgaggataca
     7921 caaaacttga accatttaac aattcctgat ccaaatacgc tgcgtgacac atgacttcga
     7981 tcgttttatc ttctctcaat atcatgagta aacttgccaa atattcttcc acactcgtat
     8041 tgatttgtcg ttttttttca tcagtttctc ctacgacatc gaaacaaggc tcaaaataat
     8101 cgacatgacg aacttcatct tttcgttcaa agttgctccg aaccggcaaa tcatattttt
     8161 ttgctaatgt aacaactacc tcctgatttt catccaaagt atgagtatga tgatgactgt
     8221 ctaaatgagt tggtatgatc cccgcatgat aaattttttg aatctgagca ttccattcct
     8281 gatacagttg atctttgtca atgttaaatg gatgctgata ataagataag gatctgaact
     8341 ctccgctttc gataagagta tcgacagttt tgaggagtgg tttgccacac gtcaatgtta
     8401 aatggactcc tacacctagc ctaggcattt cctttcttag tttcacagca tgctcgaaac
     8461 ccggcatatt tgccatcaat gtagttgatg tcaaaattcc tcgctgataa gcatccataa
     8521 tcccataatt cacaccatgg ctatagccaa aatcatccga attaaaaatc acttttccca
     8581 tttcattctc cttctttttt ctactaaaat aggcgcaacc aaataatcat agtccattta
     8641 ctatgtgtca tttggttgcg cctatagata gttacgccct tattttaata gattcttgct
     8701 agatgaattg ccaaatagat gatttcattt tcttgaatgg caagttgaaa ttgctgttcg
     8761 atgatatctt taatctttag tgctgtacaa tacgctttgc tatgtttaaa tgtttcagta
     8821 aaaaaaccaa tattttctac tggcgtttct tcctgataaa tcaagcgttt aaccaggaat
     8881 ttgatatgaa ccattaagcg atgataagtc agtgattctt tatcataaac gtttgaatag
     8941 gatttttcta gatgggacaa aatcgtggtc gtcatttcta ctatttttaa gctctttaaa
     9001 ctattaggct ctcctagctc agcattaatg atatgcatag tgatgaatcc tacttcattt
     9061 gaccctaact cggtttgata tcgatgattg attcgctcga ccacttctac tgctatatca
     9121 tattcatttg gataaaggaa tctcatttct ggcaacataa ttgctgtgac atctctatct
     9181 tctctttttc gttgaatagc aaaataaata tgatctgtca atgtaatata gatatttgaa
     9241 tctaatggag aggagatcct agctgttatc agttcaatca cttcttccgc aaaatccata
     9301 aattcgattg gaatctgcga gactatctca ctgaatcgtt tgatcttttc tttagaatct
     9361 aatacaaata atttgtcagc atcctcaaca tcaaatggct tactcacttt agcagaaaag
     9421 ccaatccctt tccccatcgc aattttttct attccatcgt catcgatcaa tacagcatta
     9481 tgattcagct ttttcatcac atacaccgtt aattcacctc ctctatatga taaaacaaaa
     9541 caacttgcag actccttcat acatattttt gtctccaaac aatttttaaa gacaaaaaac
     9601 gacctaagaa agaacccttt cttaggtcgt gcctaattga atagtagcac cctacataat
     9661 ttacaaaaat agtttaacac ataataaaag cgttatcaat atctttttta ctcgttttat
     9721 gataatactt gtatcgaatt gattt
//
LOCUS       DAFFSV010000108         9296 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFFSV010000108 DAFFSV010000000
VERSION     DAFFSV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.108"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(27..695)
                     /locus_tag="LR110_002282"
     CDS             complement(27..695)
                     /locus_tag="LR110_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL4823227.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            829..1116
                     /locus_tag="LR110_002283"
     CDS             829..1116
                     /locus_tag="LR110_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823228.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1532..3070
                     /locus_tag="LR110_002284"
     CDS             1532..3070
                     /locus_tag="LR110_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBL4823229.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3067..4032
                     /locus_tag="LR110_002285"
     CDS             3067..4032
                     /locus_tag="LR110_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4823230.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4019..5341
                     /locus_tag="LR110_002286"
     CDS             4019..5341
                     /locus_tag="LR110_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL4823231.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKNPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5404..5508
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5582..6325
                     /locus_tag="LR110_002287"
     CDS             5582..6325
                     /locus_tag="LR110_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4823232.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6340..7332
                     /locus_tag="LR110_002288"
     CDS             6340..7332
                     /locus_tag="LR110_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4823233.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7344..8081
                     /locus_tag="LR110_002289"
     CDS             7344..8081
                     /locus_tag="LR110_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4823234.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8071..8793
                     /locus_tag="LR110_002290"
     CDS             8071..8793
                     /locus_tag="LR110_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL4823235.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8877..>9296)
                     /locus_tag="LR110_002291"
     CDS             complement(8877..>9296)
                     /locus_tag="LR110_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBL4823236.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3046 a         1602 c         1965 g         2683 t
ORIGIN      
        1 ttaaataaag tagtgaaaaa taagaatcag tgctctaact gttcaagagg atcaagtggt
       61 tcaaatgttt ctgtagtctg ttttgcttcc aattggggca aaacagatcc aatttccaga
      121 tacttcactt tattcaatgt ataagataga atagaacagc taagtaatgt atacagaaaa
      181 tgtgtgagat ccaaataaag caaggagaca actaaaatac caaaagtaat caagaaaaga
      241 cttttgccaa tcttaatatg aaaatatttt tcaatggctt gataaatgat atctgttccg
      301 cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag aatacctgtc
      361 atcaaactat tgatcagtag actatctaca gcaaaatgta tatgaaggct ctcccataaa
      421 tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc gtgttttcca
      481 aaaagaaaca atgagataag caataaaggt aaattaaaaa gcagtgaagt gacagaagga
      541 gcgaatccaa aaagattatg caaaagcaaa gcaataccag taaccccacc atctgccagc
      601 ttagatggaa tattgattcc tgtcacagct aatccgtaga ttcctgcgcc taaaatgatt
      661 ccgcacaatt ctgtaaataa atttaatttt ttcatttcta gcctcttttc ttttgaaaat
      721 tttcagcaac ttctttatta tactgactta tactcttact tcaagaggaa aatgaaaaaa
      781 aattattttt ggctgtttct ttttatttat caaataagga gtggaaaaat gcgagcagca
      841 gacttattga caattcttac aaaatcaccc aaaaaaacag agtatcaagg agtttttttg
      901 gctcaagaac aaaaactggt gcctgtgaca atggtccaga taaataaagc aaatcaattt
      961 atctttttat ttgaacacgg caaagcacca cttgctatga aagacatctt tctttttttg
     1021 atgaagaacc gtgagaaagc tgtttattat cagtcaaaag aagaactcat tcctctttat
     1081 ggagtgaaag agatagaaaa taaagtggtg atataagtaa aaataagaaa acaagacgtt
     1141 tatgatgtca aaacaaccat tttttacatt tttggcaaaa aagtgtcatg atacagtata
     1201 atattcgttg aacaggccaa taaaggtgtt ctcccccacg agatttctac tttattggct
     1261 gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag atatagctga ttccaagtga
     1321 caatttcgta tagattttta tggtattcgt agtgaaaaaa cgagaaccaa aaagttgatc
     1381 cgactttgtt gcttggattc tttttttgtc agcatattta gaggtatttt tttctttttt
     1441 ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg gaaaaaggaa agaaatgttt
     1501 atacttaaag aataacagaa aggaaggtcg catgaataat tttatacaaa cattaagtga
     1561 aagaaaaggc gagttgctgg aagctgcatt tcaacatctg tccatttctc tcatttcttt
     1621 gctgattgca gcactgattg ctattccttt agcgatttgg gcagccaatc ataaaaaaat
     1681 ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg attccttcac ttgccttatt
     1741 aggtttactt atcccatttg tagggatcgg aactgttcca gcattgattg cacttgttat
     1801 ttacgctttg cttccgattt tccaaaacac gtatattggt ctagctgaaa ttgacccgtc
     1861 tattgaagaa gcagcggtag catttggtat gtccagaatg agacgcttgc tgaaagtaga
     1921 gcttcccatc gcattacctg tcattatctc aggtatccga actgccttag ttttaatcat
     1981 cggtacggct actttggcag cattgatcgg agcaggtgga ttagggacat ttattttgtt
     2041 aggtattgac cgaaatacac ctgttttgac attgattggt gcgatcagtt ctgccttgct
     2101 tgcaattata ttcagcggac tgattcgctg gttgcagcat aagaaacctc gatattccat
     2161 cattacttta gcagcagtgg tgtgtttatt tggcggcgtt tctttatatc agtctgcctt
     2221 gtttaaaaat gaaaccatca cgattgctgg aaaattagga gcggaacctg aaatcctgat
     2281 ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca gaagttgaat taaaaccgaa
     2341 ttttggcaag acgagttttt tgttcaatgc actggatcaa ggtcaaatcg atatctatcc
     2401 tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta cctgaagcaa tgaaaagtgc
     2461 tgatttgaca aaagaaaaaa catatgacga agcagaagaa ttattgaata agcaattcga
     2521 tatgacatta ttaaaaccaa tggcttacca aaatacgtat gccttggcag tcaagagatc
     2581 ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg gcagctatct ctgatcagat
     2641 caaagcggga ttcacactgg aatttattga tcgttcagat ggatataaag gaatccaaga
     2701 agcatatggc ttgaattttt caagtgtaca aagcatggag ccaagtttgc gttaccaagc
     2761 aatcaataat ggagatgtca atattgtgga tgcctattcc acagatagcc agctcaagga
     2821 gtacgatcta gtgactctag aagatgatcg agggttattc ccagcttatc aaggcgcacc
     2881 gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc gttaaagcac tgaataaatt
     2941 aagcggaaaa ataacagagg atcaaatgat tgatatgaat taccaagtca atgtcgaaaa
     3001 gaaaaagccg acagaagtag cacgaatctt cctgaaaaat gaaggattag taaaggagga
     3061 agatcagtga ctaaattaat cgagttcaaa catgtgcaaa aaaagtatga tggaaagtat
     3121 gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa tctttgtttt ggttggtcct
     3181 tccggtagtg gaaaaacaac tactttgaaa atgatcaatg gattatctaa accatcagca
     3241 ggagacatct acttcaaagg aaaatcgtta aatgaatata atctgcagaa aatgcggtgg
     3301 aatatgggat atgtgctgca gcaaatcgct ttgttcccga ccatgacagt caagcaaaat
     3361 attgaagtca tccctgaaat gttaggttgg gaaaagcaga aaagagcaga ccgtgtagat
     3421 gaacttcttc aaaaagttgg cttatctcct gatatttatc gagatcgtat gcctagagag
     3481 ttgtctggag gagaacagca acggatcggg atcatccgag ctattgctgc aagcccagat
     3541 gtgattttga tggacgagcc atttagcgca ttagatccga tttcaagaaa ctcattacaa
     3601 gaattggttt tgtccttaca cgaagaattg ggcacgacca tcgttttcgt tacgcacaat
     3661 atggaagaag caatcaagct aggtgatcgt attgcattta tgaaggatgg agaaattatc
     3721 caatgtgaca caccagagca gttattgatg aatcctaaaa acgactacgt tcggcatttc
     3781 tttgatgaac cgaaacagac gaaagagtgg cgagtagaag atttagtcgt caatggatat
     3841 ttcttaaatg aaataccaga aaatgctcgt caacaagttt gctttgatac gcctatgaaa
     3901 gaagtatatt cgcttctttc ggtgtatcca agcattgtga tagtagaaaa gcaaagaagt
     3961 attgggtcgc tgacaagtaa agaaatcttt gcttttttaa gtcgagagga ggaaggaaat
     4021 gaaaacatat gatgcaatca tcatcggttc aggtgtgagc ggcttaagtg ctgcttatgg
     4081 attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa gatctatggg gcgggacttg
     4141 tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt gcagtagagg cacgtaatcg
     4201 ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca actgccaact gggaagaact
     4261 tcaaaaattc aaacgaacat ttacggatcc agtcccagaa agccgaaaaa aacaattagc
     4321 agaagctgaa atcgatcatc tttcaggaac cgctcgtttt ttggatgatt catctattga
     4381 agtcaacgaa gaagtcttcc atgcggatta cttagttttg gcaaccgggc aaagacctac
     4441 gatcttacca gtggagggaa aggaatactt gaaaactagt gcagactttt tatcattgcc
     4501 tgtactgcct aaagaaatca tttttatcgg tggtggttat attgcttttg agctagctac
     4561 gattgccaat gctgcaggga gcaaagtaac cattgtccat cataatcaac ggccattaaa
     4621 agaatttgag gcgtctttgg tagaggaagc cgtccatcaa atggaagcat ctggcattca
     4681 gtttgctttt ggtgttgaaa cccaaaagat catttcagaa ggtactcgct atcgtttagt
     4741 cggaaaagag acggaattag tagcagatat gattttttgt gcgactggga gacagccgaa
     4801 tacagaatct ttagcacttg agcaagcgaa tatcgtcttt gataaacacg gaattgcagt
     4861 caatgattat ctgcaaacga gcaatccaaa aatttttgcg tgtggagata ttgtttcaag
     4921 gaaaaaccca aaattgacac cagtcgccac ttttgaagga aattatgtag ctaagcggat
     4981 aacggatgca acaagcgaac ctatcaaata tccgattata cctacgatcg tctacgccag
     5041 tcctaaactt gctgaagtag gagttacaaa aagtcacgcc tcttcgtcag atcaagtggt
     5101 agagatggat cttaccagtt ggttcaccta tcatcgtgtc aatgaaccag ttgctaaggc
     5161 tgaattgacc tttgaccagc aaaattattt gattggtgct gcagtgatta gcgagcaagc
     5221 agacgaattg atcgatgatt tgacactcgt catcaatcaa aaattgacta aaaaagagtt
     5281 ggatagctat atcatggggt atccgacatt ggcaagtgat ctctcctatt tattgaaata
     5341 atcagcatga taactaaagc gcttgttgct ttttccttct tgtgctacac tttaaaaaat
     5401 gaatacctgt ttctggggat gtcgtagacg acatgagagt aagttgtgaa cttagaccca
     5461 ttgaacctgt cagttaatac tggtgaaggg agaaacatag cagacgcttg aagcgggtta
     5521 ctatgtcgtg cccatttcaa gcgttttttg ttattcatat tagaagaaag gaggtaacaa
     5581 aatgaagaaa aatctttatc cagcagtcag tatggcattc attttaatta tttggcagat
     5641 tgttgtcata gtttggaaaa tacctgcctt tattttaccc ggacctgctg ctgtattttc
     5701 tgcattagcg gcagatgcac aagtattact ttcccattct gtcacgacat taaatgaagc
     5761 agtgcttggt ttggtaatag ctagcgttct ttcttttatt actgccttgg caatggatta
     5821 ttggaagttt ttagaaatgt ctttttatcc gttactagtg atttcacaaa tgttgccaat
     5881 catggtgtta gggccattac ttaccctatg gtttggtttc ggtatgcttc caaaagtgat
     5941 ccttgttgtc ttgataagct attttccgat cgtagtttct ttttcggata cattgaggaa
     6001 agtatcaaaa gagcaaatca tctttttgaa aacgatgggg gcagatacag ggaagattta
     6061 tcgaatatat aaaataccag cgggtttatc cggcttcttt tccggtttaa aagtggctgc
     6121 tacttattgt gtcagcggag cagtcgttgg agaatggcta agcgctcaag caggcttagg
     6181 gtattacatg atacgcgtaa aaaacagcta ccagatcgat aaagtttttg ctgcgatcat
     6241 ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt tcattattga aaaaagggta
     6301 ttatcgtatg ctttatattt tttaagagaa ggggatcaaa tgaaaaaatt atggctttta
     6361 ttaccgctac tgttgctttt atccgcttgt ggcactgcaa aagaaacaag tagcaaacaa
     6421 gaaataaagg atttgaaaaa agtgacactt gttttggatt atgtaccgaa tactaatcat
     6481 actggcatct acctagcaaa agaaaaaggc tattataaag aggctggttt aaatgtccaa
     6541 atcatcgaac caggagataa ttcgacaagt attggtctag taggtgcgga caaagcacaa
     6601 tttggtgtaa gttatcaaga agatgtcact tatgctcacg cagatggaca gaatatacca
     6661 gtcaaagcaa ttgctacagt gatcaaacat aatacttctg gattcgccac cctatcagac
     6721 agtaacatcc attcaccaaa agattttgaa ggaaaaacat atgcaggttg gcaaagtcca
     6781 agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg gcggggactt ttcaaaactg
     6841 acaatggtag gaagcaatgg agaagggcca gaaagcttag gaaaatcctc agatatccaa
     6901 tggtactttg aaggttggga tatgatcaaa gcaaaagaag cgggtattga actaaactat
     6961 atcccattga aagaattaga tgaacggtta gattactata ctccggtaat tattacgaat
     7021 gatcaactga tcaaaagtga tccagaattg gtacagtctt ttatggatgc cacgaaaaaa
     7081 ggctatcagg aagctattaa ggatccaaat gacagtgcta aactgttgca aaaatatgca
     7141 aaagaaaatg atcggacgtt tttagaagaa tcacaagcat tcttgtcaaa aaattatacg
     7201 gatgatcctg agaattgggg attgatggaa gaaaaagttt ggcgcaatta tacagctttt
     7261 atgcaggaaa acggattgat caagcaagat gtaccttcgc aagaactatt cactaatcag
     7321 tttataaaat aggagtgata gatatgtcaa tccaaatcag ccacttgaat gtagtcattg
     7381 aaaacctgaa aatattagaa gacatccatt tcactatccc taaagatacc tttacatcga
     7441 ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa aactttaaca ggagtccagc
     7501 ctatgacttc tggaacgatc aaagtagacg atcaaaaagt aactggtctc catacagcat
     7561 ttagttatat gccgcaagaa gatatgttac ttccttggct gagcgtctat caaaacgtta
     7621 cgttatatca aaaaatcaat catctaacaa tcaatgaaga acaagtaagg gattatctgg
     7681 atatctttgg gttaaacggc tatgaacatt ttttgccgga acaattatct ggcgggatga
     7741 agcagcgaac ggccttgtta cgaacaatca tgaatccttc ttcttacttg cttcttgatg
     7801 aaccatttgg agcgttagat gctatgacca gagggcagat gcaagactgg atgttgaagc
     7861 tgcctaaaaa agcaaagcgg actactttac ttgtgacgca tgatatcgaa gaagcaattt
     7921 atttatcaga caggattctt gtgctttccg cccgtccggc acatgtgatt gctgagattc
     7981 aagtgcctga aaaacgacgg tcacgtgaat ggctactgca gcaatcagag ttgaagcaaa
     8041 cgatttatca gttattggca ggtgaattag atgttaagtg atgctcattg tcatttagat
     8101 gggagtcaga gtttagcact ccttcagcag gaacacacga ttttgacgat catcaattgt
     8161 gattcgccag aagaatggaa ggaaaacaga cagcttgcag caagtaagac tcaagcctta
     8221 agttacggta tccatccttg gaaagcagat agctatacgt ttgaacaagt tgagcctttt
     8281 ttgaaaaaag caaggatcat cggtgaaata gggttagata atatttggac gaatgtaccg
     8341 atgactacac aaaaaaaagt atttgagcga cagcttgctt ttgcagccat aaatgagaaa
     8401 cctgttgtac tgcacacaaa aggatgcgaa aaagagatct taaattatat ccaagcttac
     8461 cctaatcact atttgatcca ttggtattct tctttggact atcaacaaga ctatattgat
     8521 ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga atccagcagt ctggcaacta
     8581 gctaaagcag tcccaatcga ccacttactt attgaaacgg atggactaca tgcagcagag
     8641 tgggctttag gggggatttt ttcagaaaaa gattatgtct cactgcttga agaacagtta
     8701 aagacaatcg ctgagatcaa agagctatca gttgaagaag ttaaaaaaaa tacttatcaa
     8761 aacttggaac gtttcattag attaggtaaa taaaaaagag atgtgacaca actagtgtcg
     8821 cacctctttt tggctttctt tgtataaaag taaactgttt gattaataaa tgaatcttag
     8881 taccaaccgt ttgcttccca gaatgctttc gccgcttccc aagaaccata acgtgaagta
     8941 acatattgtt ctgctactct ttcttggttt gcagcagagt agtcaccatt taagtaagaa
     9001 gaatctaatt ggtaacgtcc gatgtaacgt ccgttcgttg ctgtgtaaga accgcttgat
     9061 tctttttgtg cgatccattc ttttgcagag cttgtgcttg ctggagtagc agcagtttct
     9121 gttacagcag gagcaggttg tgtttcagca acaggcgctg cttgtttttc cgtttgaacc
     9181 gtttcttgta caacaggttg ttcagcttgg actggtgctt cttgaacggc tgcttgaggt
     9241 tcagcagctg cttgtgcaga gccttctgtt gggattgtta attgttgtcc tgagta
//
LOCUS       DAFFSV010000109         9295 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFFSV010000109 DAFFSV010000000
VERSION     DAFFSV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.109"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..2122
                     /locus_tag="LR110_002292"
     CDS             <1..2122
                     /locus_tag="LR110_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823237.1"
                     /translation="KKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYK
                     DDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKY
                     GTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFD
                     TIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTL
                     SIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYW
                     ERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENN
                     KEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYI
                     LVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSF
                     NTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQIN
                     PYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVS
                     FTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF
                     LINLITSIIIYKKNSFKYFKENIL"
     gene            2119..2739
                     /locus_tag="LR110_002293"
     CDS             2119..2739
                     /locus_tag="LR110_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4823238.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            2853..2990
                     /locus_tag="LR110_002294"
     CDS             2853..2990
                     /locus_tag="LR110_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823239.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESY
                     C"
     gene            3432..3812
                     /locus_tag="LR110_002295"
     CDS             3432..3812
                     /locus_tag="LR110_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBL4823240.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            3794..4708
                     /locus_tag="LR110_002296"
     CDS             3794..4708
                     /locus_tag="LR110_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL4823241.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4948..5637
                     /locus_tag="LR110_002297"
     CDS             4948..5637
                     /locus_tag="LR110_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823242.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5758..5844)
                     /locus_tag="LR110_002298"
     CDS             complement(5758..5844)
                     /locus_tag="LR110_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL4823243.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            6467..7204
                     /locus_tag="LR110_002299"
     CDS             6467..7204
                     /locus_tag="LR110_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL4823244.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            7232..7771
                     /locus_tag="LR110_002300"
     CDS             7232..7771
                     /locus_tag="LR110_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBL4823245.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            7787..7957
                     /locus_tag="LR110_002301"
     CDS             7787..7957
                     /locus_tag="LR110_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823246.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(8269..8730)
                     /locus_tag="LR110_002302"
     CDS             complement(8269..8730)
                     /locus_tag="LR110_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4823247.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            8863..9237
                     /locus_tag="LR110_002303"
     CDS             8863..9237
                     /locus_tag="LR110_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HBL4823248.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         3588 a         1411 c         1449 g         2847 t
ORIGIN      
        1 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat ttttttctac
       61 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
      121 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
      181 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
      241 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
      301 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
      361 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
      421 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
      481 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
      541 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
      601 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
      661 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
      721 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
      781 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
      841 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
      901 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
      961 aaatacccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     1021 atacaaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     1081 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     1141 cgcgccatac aattacgcaa ctatccaaga gaataataaa gaatttttta ttggacaaaa
     1201 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     1261 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     1321 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     1381 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgagg actctcctcc
     1441 tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     1501 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     1561 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     1621 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     1681 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     1741 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     1801 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     1861 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     1921 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     1981 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     2041 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     2101 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     2161 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     2221 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     2281 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     2341 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     2401 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     2461 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     2521 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     2581 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     2641 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     2701 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     2761 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     2821 aaataatcac ttaataaaaa ggaggcaccc tagtgatttt attgaaaaaa gagtatgaaa
     2881 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     2941 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     3001 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     3061 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     3121 tacacaaaaa agcatttatg ttttttgttg caaagtaaca atttcatctt cggtgaaata
     3181 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     3241 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcactaa
     3301 atacgtaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     3361 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     3421 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     3481 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     3541 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     3601 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     3661 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     3721 atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     3781 agctacaaaa gatatggcaa caattaaggt aagccgaacc acttcttgta gtcgtgcaat
     3841 taattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     3901 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     3961 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     4021 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaaca gctatttaca cacatacaga
     4081 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     4141 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     4201 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     4261 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     4321 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     4381 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     4441 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     4501 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     4561 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     4621 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     4681 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     4741 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     4801 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     4861 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     4921 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     4981 gttagtattt caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta
     5041 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
     5101 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
     5161 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
     5221 gcattagcac aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt
     5281 gtgaatcaag aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa
     5341 aacttgccag aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac
     5401 gtgcaatatg taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca
     5461 aacccttggt ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa
     5521 cgctatgaaa tcttacttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc
     5581 gccttaaact atctaaaaag tgtcaaggaa tggttagatc agcaacaaca aaattgaatt
     5641 tgatttaaaa tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc
     5701 ccagagaggt cggattctct ggggcttttt ttcaggttga tgtcgccttt attgatttta
     5761 cttcttgttt cggttattca accaataact gaataaagag acacaaactc ctacaagaag
     5821 ggggcaaatg aacagttcaa acattagata cacctccttc cagtcgcaag actgtcggat
     5881 aggcgacaag ttattatatc ttaaaaaatt aaatttgtgt ggtgttttta agtatcactt
     5941 gttttagggt tgttagggct tgccctgacc gcctgtaagg cgcttctcga ctagattaag
     6001 ggaaattaat caagaattgt cttagaacgg cttagacaag ctttcagagt ctcacacctc
     6061 ctctttgaac aacggtttta gcaaaggagc cggaggcgac tgcgcagtat aatatgagag
     6121 catagcgatc acaccttttt agcgaccgta gggagcaagt tttccagttt agaaaaattc
     6181 gcgaaccttt ttggaggaaa aagtaggagt tttttaggcg ggaaattcaa tgtgctctta
     6241 agatatactg ttagatatac tgttagatat actgttaata ataactgtta gggattagca
     6301 accttatggg agagtaagta ttgacatagg tagtggggga taaattgtca cactatgggg
     6361 gataaattgt cacactatgg gggataaatt gtcacactat gggggataaa ttgtgtttta
     6421 catcctctta tgtctatgat aacataattt attgaggtga tttttattga gtaacgatat
     6481 agttcgatat aataatggat taaattctgt ccctttaaga aaatttaccc ctatagatat
     6541 ggatttattt tggggaatat gttctaaaat gaaaagaaaa ggtacaaaag aagaaatttt
     6601 tacatttaaa caaataaaag aattaattga ttacaatata aatgcacgtg gcgaccgttt
     6661 tgctaatgat ttaataaaaa tgtcaaaaaa aatcattact ttgtctttta tttatgaaga
     6721 cgaaaaaaca tatgaacaat taaatctgtt tcaaaaattt aaaatcgata aagtgaatga
     6781 aacattatta ataaaggttt ctgaagaatt tgaatatata cttaattcaa tcggaactaa
     6841 ttttactcgt ttcgaattag aaaatatgac aaacttaaag agtagctacg ttaaagagtt
     6901 atatcgccaa cttatggctc ataaagacag aagatctaaa aaaggtcact ggtttgtaaa
     6961 aatagaagat tttagagctc tattagcaat accagaaagt tatcgcatga gagatattga
     7021 taaacaaatt tttgcccaag cgaaaaaaga atttttaaac gttaattcaa ctcaccctgc
     7081 tatttttaaa acttttgaaa tcaaaaaaat taaagcctac aaaggaaata agataggatc
     7141 tctgcaaatt tattttgaag aaaatatacc ttcaattacc ttagataatt ggttagagaa
     7201 ataataccta acaatttagg aggcaattat catgcaaccc aaagaaacct ttacaataaa
     7261 ggaattatct gaattatttg aaataagtag gcaagcaatc tctaaacatg ttgataaatt
     7321 agacccgtcc ttattagcta aaaatgagcg tggttataag gttgtattac gttctggagt
     7381 attgcaactt gctagcaact taggtaacca aaggttacta tataggttgc atgagtatga
     7441 acaagaaaac gataaaaacg tttatatatc aagcaaaaac gtgattgtaa acgagttgct
     7501 agataagttg cgtgaagaaa atgaattttt acgagaacaa ctttccctca aagagcaaaa
     7561 taattctaat atgcaaaaat tactcgacca gcaacaacag ctcaccctac aggcaaatca
     7621 acagatcgaa aaactgcaag aacagttaca attagcttat acggaagaaa ctccagaaga
     7681 tgaatcaact actattcttt ctgaagaaac agaatcgata gaaaaacaca caaaaaagaa
     7741 gaagcaaaaa tggtggcaac tttggaaata actggaggga acattcatga caagaaaaaa
     7801 tcttctattc ggttctattt tactaggaat tctaggcttc agtccgatgt tattgtcagt
     7861 aatcaataaa gggaataaac atggtgaatt gaaattgaat ccaaattatt acaacagtcc
     7921 taacgaagaa ttgtttgtaa aagagacgga taaataataa aaaagactgc tcttccgagc
     7981 agccagttaa aaaataatga gtgtttattc ttgaaaaagc acgcgcttat gaagcgctac
     8041 caaaaattta ccatatttta caaaaaataa caagtcttaa aaaaatgaca gataaaaatg
     8101 cacaaagaat tagatataga taaccgcgaa aaatcaagct ttagaaagtt gcttttagct
     8161 agaccaatta aaaatcacaa agtgagcgtc tctatccatt ggtagagggg ctacttggca
     8221 tagtgtacat tatgtcaact atgcaattaa ataactaact acttcatatt agcttaataa
     8281 aataatcaaa ataactaggg ggaaaaacat tgtataaaaa aatttttcat tttttaaata
     8341 agaatagtaa atataaaaat aatttaagat atctattact attaatccag atagagcaaa
     8401 ccaagtaaat attccatgtg taaagctaaa aaaattcatt tgaatcaatt caaataaacc
     8461 taatatagta acaaatatat taaaaataat agctgtcaat accagtaaat tatttttttt
     8521 attttttgaa ttatcttcat ttaaaataga atttatgtta gtactataaa attcacaaat
     8581 tttttttagt gtctctatat ctggtgttgt tttgttattt tcccactttg aaatagtttg
     8641 acgtgtaacg ttaaattttc ttgccacata ttcttgagta tacccttttt tcactcttaa
     8701 ttctcttagc ttagatccta aatcctccat tttttctcct atgtaaacga ttatttacat
     8761 aaaattttat cataaaaata ctaacgcgcc aaaaaagttc tttattttat aaaacacact
     8821 aaaataaaat aattctagta aaaaagatag gagaaataag ttatgaaaaa atttttatgt
     8881 ctttctgctt taatgggtgt cttattaact agtggaggaa tagctagtgc cacagaagct
     8941 ttaaatctag atgtacccga agaccatacc gccatttacg gaggaggaat ggaagctaca
     9001 gaagaaggat tttcttctag taaattgaga tatgctgctg gcggcgggga tttcaactgt
     9061 ggggttaatg gatttaaagt ttatgcaaat tactaccatg ctagagctaa acatagtgca
     9121 acagctaaaa atggacgagg cggtcaagta cgatcagttc agaaagctgg agtaagagct
     9181 tatgcaacat gcaatgccac acttactgga aatactggtt ggtggaatgt ttattaaaaa
     9241 agagagttta aactctcttt tttatttaca tttgaaagga aattcaaaat atgtt
//
LOCUS       DAFFSV010000110         9135 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFFSV010000110 DAFFSV010000000
VERSION     DAFFSV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.110"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            14..2791
                     /locus_tag="LR110_002304"
     CDS             14..2791
                     /locus_tag="LR110_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL4823249.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            2872..3291
                     /locus_tag="LR110_002305"
     CDS             2872..3291
                     /locus_tag="LR110_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBL4823250.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3303..3773
                     /locus_tag="LR110_002306"
     CDS             3303..3773
                     /locus_tag="LR110_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4823251.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3790..4569
                     /locus_tag="LR110_002307"
     CDS             3790..4569
                     /locus_tag="LR110_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4823252.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4556..5377
                     /locus_tag="LR110_002308"
     CDS             4556..5377
                     /locus_tag="LR110_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL4823253.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5394..6443
                     /locus_tag="LR110_002309"
     CDS             5394..6443
                     /locus_tag="LR110_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL4823254.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6456..7460
                     /locus_tag="LR110_002310"
     CDS             6456..7460
                     /locus_tag="LR110_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL4823255.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8093..9112
                     /locus_tag="LR110_002311"
     CDS             8093..9112
                     /locus_tag="LR110_002311"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL4823256.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3042 a         1532 c         1938 g         2623 t
ORIGIN      
        1 tgatggagga ggggtgaata agttgttgaa gaaggaaatt ttatgttact tagagaacca
       61 gacagccttt ttcgatttag aacatatgaa cgaggtcttt acagcacaaa ggttagctga
      121 tcattttggg gtaaaaagaa acacagtcag tcattatttg aaccagttaa atgatgagca
      181 actactagtc aaaatcaatt ctcgaccagt gatttatttc cacaaagaag cttttgaaaa
      241 acaatttttt aagttacgca cgaattttta tttttctatc gatgatctca aagaggaaca
      301 gccttttttc gcgcaaccaa aagacttatt ctcttttatg atcggatacg acgccagttt
      361 gaaagaaagt atcgaacaaa tcaaaacagc tctttactat ccagatggcg gattaccact
      421 tttgattaca ggcgaaagtg gcacaggtaa aagttattta gttaatttgg tctatcagta
      481 ttgtctgctt catgatctgc tagaagattc tgcaccattc atcactttta attgtgcaca
      541 atatgcagat aatcctgaac ttttgactag caatttgttt ggacatgtta agggagcgta
      601 cactggtgca gaagaaaata aaaaaggggc ttttgaagca gcagatggtg ggatcttgtt
      661 tttagacgag gtacatcgct taacaccaga aggtcaggaa aaactgttca cctatcttga
      721 tcaaggtgtg atttatcgta tgggagaacc taatttgtca cgccggatca aaacacgtct
      781 cttttttgct acgactgaag agattactag tcatttctta acaactttca ttcgaagaat
      841 acctatccga atcgaactac ctgcacttaa tcagcgtagt cgtaatgaac ggctagaact
      901 gatttactct ttttttctcg aagaacaaag aaaaatgggg agccctttgt cagtcagtgg
      961 accagctttg tcacttttaa caagtcagaa attatccgga aacataggag agctgaaaaa
     1021 tatcgtcaaa gtttcagcag caaaagcata tgctgaacag cgtgaacagc ctgaaatcca
     1081 tgtcacgatc catcatttgc aaaaagaact acttgcgcag cctattacta aaaatacagc
     1141 acagcaagga atctatatta cgcaagacca gaccttagag caattaatgg agaagcaaca
     1201 gcccgaacag cagcgaatcg ttaaaagttt tgagcaaatc ctaattgatt tcaagaaaaa
     1261 taactgtctg ctatattctt gcgaaggaaa attaaagcaa gaagtgattc aattatttga
     1321 ttttttgctg tttgagacca gtagacaaga aaaacacgag ctgctggttt attggacaca
     1381 atctatccga gagacctttc gacaaatgga aactgcctat caaatcaaat tcgatggaaa
     1441 cagtgtctat gccttgagtt actatctcta tcagcgaagc actatcaaat ggttaccgga
     1501 agaacatgat attatccaac taatcaaaga attagaacaa caagtttctc agtcttatcc
     1561 atctagttat cactatgtcc aacgaattct ggaattaagt aagatcaaat tagatattga
     1621 agacacaagt atggatcgga ttttactgac gatctatcta aaaaaagcga aatggtcaaa
     1681 agaaaaacga ctgccaaaag cgatgattgc tgctcacggt tatgcaactg ctagcagtat
     1741 ggcaaatgta gtaaatcgtt tattacaaga ggatctattt gaatcatttg atatgccact
     1801 agatgtcaca ccacagcaga ttgcttcaga aatccttgat tattgcgaaa acagcgatgt
     1861 ttcaaatgga ttgatcatat tagtagacat gggatctctg aaagaaatcc atcagttttt
     1921 taaaaaacag ttatccgtac cgcttttgat tttgaataat gtgacgacac ctttagcaat
     1981 cacagtaggg gaatgcctgc aaaaaaatgt atcattagaa gagatagcag aagagactgt
     2041 tcgacagatc cagccagaat ggcgactgct ctatccagag gaaaacaaac ccaaagcact
     2101 cattactact tgcttcactg gaatcggtac ggcggtccac ttaagtgaac tgctagaaaa
     2161 aagcttacct acggcttgtc agttgaaaat catcccatat gaatatcagc aattaaaaga
     2221 taagaaaaac agtgacccat tattttccat atacgaagtc gtaggaatga ttggcaccac
     2281 agatcctgat atcaccaata tcgactatct ttcattagaa gacctcatct cgggggaaaa
     2341 gatgtctgta ctagctgaat ggttggacac tactatgaat gcttcagaga aggaaatatt
     2401 caatcagcgc atcattcgta atttttcatt ggaaaaagta ctggattccg tgactattct
     2461 agatacagag aaggtgatgg gagaaatcga tcattttatg agagaattag aggtgcagtt
     2521 gaatcgaagc atcagcaatc ccaaaaaatt agcgttgtat gtccatgtga gctgtctcat
     2581 tgaacgattg atccgacaaa tgccaatcga aacctatcaa ggattagaga aatggaagca
     2641 gtgtcaaaaa gaagggttat ctgctattaa atctgcgttt agtgtcattg aagagcatta
     2701 tagtgtcatg atccccaatt ctgagattgc ctatatctat gatattttga gtgaagatac
     2761 agagtttctc tcgatagaag aagaattttg aacataagca agttgttgcc actaattttg
     2821 gcatgcaact tgctttttta gtatgtgtaa gtgaaaagag gagatgaaaa agtggataga
     2881 gaaattatct tagcttccca tggaaaattt gcttcaggga tattagattc attagagctg
     2941 atctatggga aaaatcattc tattactgta ttcgattgct atactgatga gaattttgat
     3001 ttagcggaaa cagtaaggca gctttttatg aggtacaaag ataaagaaat cattgttctg
     3061 acggatcttt ttggcggaag tgtcaacaac gaattcttac aatatatcaa tcgggatcat
     3121 gtctatctcg ttgccggatt gaatttgcca ctattgattg agctagtatc tcggttggac
     3181 gctgaagtag agacaactgt tttgatccag caggtgctaa ctgattcaaa aaaaatgatc
     3241 cagttttgta acgagcggct caatcagaaa atagaagaag acgaatttta ggaggaagaa
     3301 aaatgattgt attgacaaga gtcgaccatc ggttattaca cggacaagta gctttttcat
     3361 ggacacaaac aataggcgca gactgtattt tgattgctaa tgatgatgta cctacgaatg
     3421 agatacgaaa gacaacgatc aaattagcca agccacaagg agtcaaacta gtgatcaaat
     3481 caattgatga ctcgattgcc gcgttgaaaa gcggggtgac agataaatat aagttattca
     3541 tcgtagtgga atcgatcgag gatgcatata agctggcaga agcgtatcca gaaatcaaaa
     3601 ccatcaactt aggagggatc aaggcaaaac ctggtactcg cagcatcgga aaagcggtaa
     3661 atattttacc agaagaggag gaacgcttga aacgattaat tgacaaacaa atagaagtag
     3721 aaatccgtca agtaccagca gataaaaaag tggatgtaag agaagttttg taagaaagga
     3781 agaacctcaa tgattgtaca agcagtctta ttaggaattg ttgcatttgt cgcacaatca
     3841 gaatatgcac tagggacatc gttgctttct cgtccgattg tgacggggct gtttacagga
     3901 atcgtgttag gtgatgtaaa aactgggatc attatgggcg caactttaga gttagcattt
     3961 atcggatcgt tctcagtagg tgcttcgatt ccacctgatg ttgtgaccgg tgggatttta
     4021 gggacggctt ttgctattac agctggagcc ggtacagaaa ccgcgctttt actaggtttg
     4081 ccgattgcta cgttgacatt gatattgaaa aatatttact tagggctttt gattccaatt
     4141 atgaaccata aagcagacaa ttatgcagaa gaaggaaatt ataaaggagt cgagcatatg
     4201 cacttgcttg ccggtatcgg tctttccttg atgcttggtg ttgtggtaat ggtttcttat
     4261 atggtgggaa gcaatacgat cggaaatcta ttaaatatga ttcctgactt cgttcaaaag
     4321 gggttgtcag tagctacagg gttgattcca gctcttggtt ttgcgatgct tgctcgtttg
     4381 ctgatcaaca aacaagttgc accatatttt ttcttaggtt ttgctatagc ttcttactta
     4441 gagattcctg tgaccggagt agctattttt ggtgcgattt tagcagttgt ggtagtcaat
     4501 attatgaatg tccgtcaact accacttcaa acaactgcag gggaggtaga agaagatgaa
     4561 gaattctgag atcaaaaaca gcaaaataac gaaaaaagag ctaaatcacg tattttggcg
     4621 ttcttttcaa atggagtttt cttggaatta tgaacgtcag atgaatatgg cttatgttta
     4681 tgcaatgatt ccgattttga aaaagttata tcagtcgaaa gaagaaatgg cagcagcttt
     4741 gaaacggcat ctagagtttt ttaacacgac cccacatatc gttacactga ttttaggaat
     4801 cacggctgca atggaggaag aaaataagga tgatcctgaa tttgatgtga ctgcgatcga
     4861 tagtatcaaa acatctttga tgggacctct tgcagggtta ggagattctt tcttttgggg
     4921 aactttgcga ttgattgcca caggtgtggg tacctcttta gccatgcaag gaaatatttt
     4981 aggccctatc ttgttcattt tgattttcaa tattccgcac attctgtttc gctatctagc
     5041 aacaggttgg ggatatcgct taggaacggg ttttttgaaa aagatccagg caaacggtat
     5101 gatggaaagt ttgacattag gcgcgtctat tattggactg atggtcgttg gagcaatgac
     5161 tgccacgatg atcaccatca atattccttt aaaaatcggt tcaggagaaa atgctgtgac
     5221 tgttcaaagt atttttgatg atatcgttcc aaatatctta tgtcttggag catttggcat
     5281 agtgttttat ctattgaaga aagaagtgaa accattaacg atcttgatcg gactagcttt
     5341 ctttggaatt tttggttcgt ggatagggat tttttaaaaa aggagcagag aaaatgttta
     5401 cgatgcaaga ttatatctat gaagaaaagg aagtactaag tacgatcttg aaaaagaatg
     5461 atttttcgac aagagaacat atgaaaaaaa cagtgaattt actgatctta gcaacgggtt
     5521 catcttataa cgcttgttta gcagcgaagc cagcattgga atcttatggt gatcttactg
     5581 tcgatattca agaacctttt tattttttca attatggaaa actttctcct tcaatcgata
     5641 ctgtgattgc tgtgtcacaa agcgggaaaa gtgcttctac tgttgaagca gtaaaaatga
     5701 tacaaaaaca agggttacct gttgttgcta tcacaaatga cgtgcaaagt ccacttgctt
     5761 tagaagctgc acaaattatt gatttaggtg ccggggtaga atcagtaggc ttcgttacaa
     5821 aaggctattc tgcgactgtt ttacagttac tactcttagg gataggaatt ggaataagta
     5881 aaaataaaat cagcaaaaaa atagaacaag actacatgca acagttgcgc aaaataatca
     5941 accatttacc agcgattatt caaaaaacgg aagaattttt tgatggatat caaagtttat
     6001 ttcgtttagc acagcgtttc gtcaccattg gatatggtcc aaactgggga acagctaaag
     6061 aagcagagac gaaattgact gaaacaatcc gcgttccgtc acaaggattt gagttagaag
     6121 cttatatgca cggcccatac ctggaggcag atgcttcgca tttgctattc tttattgaag
     6181 gggaaagtgt aaataaggaa cgttcgcaaa agttgcaaag atatatgagt cggtacgttg
     6241 gagagactct caccattacc acaaaaaaag caagaaacga gaaaacactt ggattagcaa
     6301 tagaatgtga tgaatatctg tctgtattag cgcttgtcgt tccgtttcaa ctattcgctt
     6361 ataaaaccgc agtagctaaa ggtattgact tgaacaaaaa gatttttgaa gactttgata
     6421 cagtcttgaa aagtaaatta taagaggtgg aaatcatgct gaaattcaat gaagaagaac
     6481 aaatcaaagc aataaaaggc gcactagctt tacgtccgca agtagaagaa atcatcgata
     6541 aactttatgc ggaaaaattt gacgctgtct attatctagg aatcggtgga acgtatgctt
     6601 cctcgatgca agctgtcact tatatgaatg ggaaaagcaa cttgcctgtc tttgtacagc
     6661 atgctgcaga atattacact actggaaaca aaagattaac aaaagattct atcgttattt
     6721 tgtcctctgt taccggtaca actcaagaag tagtgaaagc agtagaacaa atcaagaaag
     6781 taggagcgac actgattgga tttatcgata aagcgaacag taagttgtct cagctatgcg
     6841 attttgtggt cacttatcca gctcctggaa cagaacaaat caaatttttt atggcagcag
     6901 atcgattgat gtatttgaat ggagaattcg aagcttattc agaatactat gaacagctcg
     6961 aacgttattt accaacaggg ttagtggaag cagagaaaaa agcagatgca tttggcttat
     7021 catttgcaga aaaacatcga cgtgatagta tgcattattt tattggagca gggaatcaat
     7081 ggggagctgt ttattcatat gccatgtgtt actgggaaga acaaagctgg cttccatcga
     7141 aatctatcca tgctgccgag tttcttcacg ggacgttaga gatcgtagaa gaaacaaccc
     7201 cagtcacatt atttttaggc gaagatgaac agagagtgtt atctgaacgt gtggctaaac
     7261 ttttgccaaa aatttgcagc aattatacat tgatagacac aaaagactat ccagtagaag
     7321 gtatcagtga aaaatatcgc ggacgtgttt tgtctttctt attgatgcat gtagtgacac
     7381 aacgcatcga tgctcatgtg gagcagttga actgccatcc attagagata cgccgctact
     7441 atcgtcagtt tgattattag gatagactgg ataaaaagaa tataaaaaat aaagagagca
     7501 gcgataactg ctccctttat ttttttaaaa tattggcatc aattacgtta taaaacacat
     7561 tttttgcatt atctacagtt caacttataa tctgtttaat gggacatgtg ggtgccaacc
     7621 attttttgtt atgcaatagt actatttagt tgttgcatgg atccgtagta aaagtccaag
     7681 tacaacgagt tgcgccagtt tacatagcgt tttgtatcca gaatgttgac taaaacaaaa
     7741 gttcctatgt caaccgtcaa cagagacaat ctgtccgttg caggacctga cagatttaga
     7801 aatgttcaat ctgtctctta tgtattttaa atcaatactc actaggagaa gtttcctaac
     7861 ctcctaatag taatgctggt agtaacattg tcaaaactgc ttcctcttag gaacaagcaa
     7921 agcataacga gtgtaaaaat aaaatgtaga ctattgtaac atatcattga ttatttcaag
     7981 taaaaacgat aaaagaagta aacaaacact taaaaacaat ggaaattagt cccaacttta
     8041 agaaatgtat actataatgg aaattatcaa ttatttattt ggaggaatat ccatgaaaca
     8101 aaaaatcgca gttcttggtc ctggatcttg gggcactgct ttagcacaaa cattggcaga
     8161 aaacgggcat gatgtccgta tttggggaaa tgtacctgaa cagattgatg aaatcaataa
     8221 gtatcataca aatcagcatt ttttacctga tttgacgatc ccagaatcga ttatcggata
     8281 taaagaattg gcagaagcag tggataacgc agatgctatt ttatttgttg ttccaacaaa
     8341 agcgattcgt tcagtcgcac aggaacttat tggtaaaatg aatactaagc caatcattat
     8401 acatgcaagt aaaggactag aacaagatac gcataaacga atctcagaag ttttggctga
     8461 agaaattcca gaagaaaaaa gacaggcaat tgttgtatta tctggtccta gccatgctga
     8521 ggaagtggca gtacatgata tcacaacaat tacagctgca agtattgatc aaaaagcagc
     8581 agcctatgta caaaaactgt tcatgaatga ttatttcagg atttatacaa ataatgatgt
     8641 gattggcgta gaaactggtg cagcactgaa aaatatcatt gcaattggtg ccggcgctat
     8701 ccatggatta ggattcggag atgatgcaaa agcagcaatc atgactcgag gattggcaga
     8761 aatcagccgt cttggtgtcg cgatgggtgc caatcctctg acatttatcg gtttgagtgg
     8821 tgtgggggac ttgatcgtta cttgtacaag tgttcattct cgcaattggc gtgccggtaa
     8881 cttgttagga cagggacata agctggaaga agttctagaa aacatgggaa tggtcgttga
     8941 gggtgtggca acgacaaaag cagcagtaga gctagctcaa cagctaggtg ttgagatgcc
     9001 aattactcaa acaatctata atgttttgta taatggagaa gatatcaaaa aagcagcaaa
     9061 agaaatcatg ttacgtgacg gaaaaatgga gaatgaattt tctcttcgtt aaatagatga
     9121 tcaatagtta atcgt
//
LOCUS       DAFFSV010000111         9072 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFFSV010000111 DAFFSV010000000
VERSION     DAFFSV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.111"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            50..274
                     /locus_tag="LR110_002312"
     CDS             50..274
                     /locus_tag="LR110_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823257.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            289..726
                     /locus_tag="LR110_002313"
     CDS             289..726
                     /locus_tag="LR110_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823258.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            719..1426
                     /locus_tag="LR110_002314"
     CDS             719..1426
                     /locus_tag="LR110_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823259.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            1807..1986
                     /locus_tag="LR110_002315"
     CDS             1807..1986
                     /locus_tag="LR110_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823260.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            2079..2264
                     /locus_tag="LR110_002316"
     CDS             2079..2264
                     /locus_tag="LR110_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBL4823261.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            2298..2729
                     /locus_tag="LR110_002317"
     CDS             2298..2729
                     /locus_tag="LR110_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBL4823262.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            3666..4940
                     /locus_tag="LR110_002318"
     CDS             3666..4940
                     /locus_tag="LR110_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4823263.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            4994..6421
                     /locus_tag="LR110_002319"
     CDS             4994..6421
                     /locus_tag="LR110_002319"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBL4823264.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            6434..7306
                     /locus_tag="LR110_002320"
     CDS             6434..7306
                     /locus_tag="LR110_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL4823265.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            7401..8396
                     /locus_tag="LR110_002321"
     CDS             7401..8396
                     /locus_tag="LR110_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4823266.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
BASE COUNT         3174 a         1314 c         1756 g         2828 t
ORIGIN      
        1 tgtcttatgg cgattacatt ttgattgatg agaaaggaaa gaaacgctga tgaaaacaca
       61 acacaaatta ttgaatgtaa aagaagtcat ttctgaaaaa gtagcagaag ctttacttat
      121 tccgcggtac tggacgatag aaaccccttt agtagaggac gcaccgacta tgctatttga
      181 agacctagtg aagattgcag gatatgaact gcattatcaa gaaaacttgg aaacttatga
      241 aaacgaatat tggttaacca aattagagaa ctaaaaagga gaaaaaatat ggctcagaat
      301 tactatgacg aatttgtcaa attacctttg gataaaatgg cacaaaaaat ggaggatatg
      361 acgttcctat accatgaaac acgtgtaccc aaaaaacatt acaaggaaaa attgtcagtc
      421 gcagtggaag aaatgattga atcaggtgtc gaaatgaatc tcattgcgac gtactatcga
      481 acattggaag aactgaaaaa acaaaatgga aaatggtttt tccaagcctt actatgcttg
      541 gaagcaggcg tcaaaccaag cacaatcaaa ccttctgaat accaagcttt agaactaacg
      601 tatggaaaat ttatcgaaac gaaaaaagcc aagactgttt caagtgaatg gttagactac
      661 tttgaaaatg tcaacaagta tggcgcatgt tacatcatga aaaaggagga caacgaaaat
      721 gagtaatgtg gtttatcaac taacagtgga agatgtttct cactttttaa caaacaaaga
      781 acatttgaat gaagtagtca gacggattca aaaagacttt agttgtatgg aagaaatgaa
      841 gtactttatt caaagagtgg aagaacagtt gtatatggaa agcgaaacga gtcaaacgaa
      901 ggtcacagtc agccataatc atttagacgt gtataaacgt cgtcctgaaa cggtaaaaaa
      961 aggaacagag cttctacgac tagaaacaga aaaatacatc atgaatgtgt acctagaaag
     1021 gagtgagctg gtcgaaaact ttgtcagcta cacgatttgt aggaaacact attactcaga
     1081 atcagaatat tattcaattt ttgagagcat ggaattaatg gaagaagaaa tggaagagct
     1141 aaacagcaaa aatgcgacgg tagacgatta ttaccttgta tttactagat ttctgacgtg
     1201 tgggacggaa tctatcgtgt taatgggaat ggattatgaa ctcgctgtac ccgaagcatt
     1261 tttgagggaa tatgtagaag aagtagataa cccaggcgac gactggtaca cgactttaac
     1321 tgatttttta gatacctaca cttacgacga tacagaagaa atccaaaatg agcttgactg
     1381 taaacgaatg ccatatcaac gatttgaaag gaaaaaaggt caataaccat ttataccgaa
     1441 aagtgagaat gtgtgaaata agaacaaaaa accttgaaat taagggaatg gttcttattt
     1501 cgcaccaatc gctccaaccg cccccgccgc tcaggtggtg cgaaataagc gggcaacgac
     1561 cgataaccta agagaaagaa ggaaatccat atgaataaaa tcattcctct gttgttcgta
     1621 ctgtttgttg gcgagatact tatgcgtcat gctgcagctg tacatttaaa cttcgtagta
     1681 ttcttaggaa tactggtatt ttgtttgatc ttttggcgat ttgtattagc ccttgtgagt
     1741 ctactattca agatgattgc agccatattt ttcatagggt tatttcgatg aaaggagaga
     1801 gtactaatgc caattgcaat aggaaataaa cgtttacccg tgacgttgga tgaaaagaga
     1861 caaaaagaat tgcaggaact caaacagaag tacggcaaaa gtgaatccag gattatgtgt
     1921 attgcgttag atttattgat tgcacaagaa aaagcagggt ttgatgtacc agcactcaaa
     1981 aagtgacgtc acttttcatt tgaagaaaca aaagtgatag cactttacgc acgtgtcgat
     2041 acgtgttatg attattttgt aaggaggtat agtgcacaat gccacttacg ggaaaagaaa
     2101 tgcttaagtt attgaaaaaa aatggctggg ttgagcgaag gcaagaggga tctcatcatc
     2161 atctttataa ggatggtgtt agaatcactg ttcctgttca tgccaatcaa gatcttggcc
     2221 gaggacttga gcaaaaaatt ttaaaggatg cgggattgaa ataatctcgc attcgattaa
     2281 agaaaggatt gactgaaatg ttaagtgagc ctttagcaaa aacctatcct gctattttta
     2341 gcccagaaga aggtggtggg tactttattg aatttcctga tgttcaaggt gcttacacag
     2401 gaattaacga agacgatata tcttatggaa ttgctatggc acaagaggtg ttaggtatgg
     2461 ttttagcaga ttatattgag catgaagatc tacttccaga accaacacca atcaataaga
     2521 tttctgttga agatgattct tttactactt taattcgtgt agatgttgcc aaatatttga
     2581 aagacacaga acttgtcaaa aaaaccttaa caattcctca atgggcggat aagctaggca
     2641 aaagggctgg cataaatttt tcagtccttc taacagaatc aattgcagat aaagcagata
     2701 acattcttcg ttctggaaga aataattaga actcaaaaga gtcagaaact taaaaatctt
     2761 tggcttcttt ttttactaca aagatggctt ctttaaatta tttgcctgat tgatttaaaa
     2821 aaattgcgca aagactacga gctaattaag ttgctcgtgg tctttcttta tgaaaacttt
     2881 ttagatctag ataaaaacat tgagacactt tgaaaaattt ttttaatagt aatgggcgct
     2941 tctatgtcaa gctgattctt ctttcggtaa attaatttta cttagaatat cactatcttg
     3001 ataaaaaatg gtgtattcac aaattctggc ataaaaaata cctactttct agttaaggtt
     3061 cctgaataag tcgcaatcca attatttcca gctgatttat aactttttaa atataatttt
     3121 ccataatacc aataaccatt acttttttct gtccaatttc gttattgaga agatctagaa
     3181 ggccagccaa ctttatcaaa aatcatcatt cctgtcactc gtatcgtttt agaagctgct
     3241 cgagtaagta atggagaaac tgttaaataa ttttaagtaa cagttaagtc gttagttggg
     3301 ttatcaatgg ctattccgtc cttatctgaa cctcgaaacc caatcataga aaaagttttt
     3361 tgaacaagtt ttaacagata aatcaagtta tatactagaa aattcgtaaa atatattttt
     3421 aatgtcataa tcgtttgatt tttctggatt gacatttatt tataaaaaat agtgccatta
     3481 aaaaagaaaa gtgacacacc aataaaagtc atatcctgtg acattatatg gcattatcca
     3541 aatagaataa ccggttgaca tgtaagcgga ttccttttat aattttagga gtataagtcc
     3601 ttttctaaaa atattaaccg gttatcatga aaggggatac aaatatatcg ttggaggata
     3661 aaaatatgtc aaaggttaac gtatttaaaa atcaatccta tttacaaagt tcagctacat
     3721 tattactatt ttttgcttct tggggtgttt ggtggtcatt ttttcaactt tggctaacat
     3781 ctgaatcaaa tggtttaggg ttatctggca gtgctgtagg aacagtattc tcggcaaatt
     3841 cgttagttac cttaattttg atgtttattt atggaacatt acaagataaa ttgtatatta
     3901 aacgaaattt attaattttt gcttctgtat tagcgacact tgttggacca ttttttatat
     3961 ggatatacgg gccattgcta gataacaatt ttaatttagg cattattatg ggagcactat
     4021 ttttgtcagc tggatattta gcttctgtag gagtttttga agctgtgtca gaaaggttta
     4081 gtcgtttatt tggctttgaa tatggacaag caagggcgtg gggatcattt ggttatgcct
     4141 tggtagcgct tttggcagga tttttatttg taaaaaatcc tcatttaaac ttttgggcgg
     4201 gatctttctt tggttcttta ctattgttaa atttattatt ttggaaccct aaagttgaac
     4261 gggaagcaaa tcaaaatttt aatcaagaac aagctgaatc aaatagtatt ccttctttaa
     4321 aagaaatgtt tgatctaatg aaactgcctc aattatggac gataatcatc tttattgttt
     4381 ttacatggac attttatacg gtattcgatc aacaaatgtt tccgggattt tatactggtt
     4441 tgttttcaac atcagctaat ggtgaaaaaa tatatgggac attgaatgct attcaagtat
     4501 tttgtgaagc gttaatgatg ggaattgttc caatcattat gagaaaatta ggggttcgaa
     4561 atactttgtt attaggtgta accattatgt gtgtacgaat tggattgtgc gggtttgcct
     4621 cgacaccatt atctgtttca tgcataaaaa tgttgcatgc tttagaagta ccattattta
     4681 cattaccaat gtttcgctat tttacacttc attttgatac aaagctatca gcaaccctct
     4741 atatgatagg atttcagata gctgctcaaa ttgggcaagt gattttatca acaccattgg
     4801 gaatattaag agacaacgtt ggctatcaac caacatttaa aattatttct cttattgtat
     4861 tactagcagg catatatgca ttctttattc ttaaacaaga tgatagagat gttcaagggg
     4921 atccatttat tcgaggataa tggaaaccaa cagaataaaa aacaatttta tctagaaagt
     4981 gagcgaatat attatgacaa aaagagctat agaattaaca aatacacgtt atcgtttggg
     5041 atatcatgtg tctgcaccat caggatggat taatgatcca aatggatttt gctattttga
     5101 tgggtattat catgtgtttt atcaacatca tccatatagt gctgaatggg gaccaatgca
     5161 ctgggcacat gctagaagta aggatttagt tcattgggaa tctttaccac ttgctttgac
     5221 tcctggagat caagaagatg aaggtggttg tttttcagga agcgctattg aaaaaaatgg
     5281 tgtgttgtat ctgttctata caggacatca ctattatgga gacaatgatc cagatcattt
     5341 ttggcaaaat caaaacatgg cgtatagtac ggatggcatt cactttacta aatatgaaaa
     5401 aaatcctgta atagcaaaag caccagaaga taacacacat catttcagag atccaaaagt
     5461 atggaaacac gatgaaaaat attacatggt tttgggtagt caagaaaaag atggattagg
     5521 tcgtgctatt gtttatcagt cgttagattt attaaattgg gattacattg gaccaatttc
     5581 taaagcgaag gaagtactta cagaaggatt tatgtgggaa tgcccagact tttttgaatt
     5641 aaatggaaaa tctattcttt tattatcccc tcagggaatt gaagcaaatg gaaaagatta
     5701 tttaaatcta tatgaaacag gctactttat cggtgattat gattatgaga cagcaaaatt
     5761 cattagagga aatttttatg aattagataa gggacacgat ttctatgcaa cacaaacaac
     5821 tttatctcca gatggtagac gtattgttat ggcttggatg gatatgtggg aaagccctat
     5881 gcctgaaaaa gtagacggat gggcaggagc attaacgctt cctcgagaat tagagttaat
     5941 tggagatcat ttgtatatga aacctgtgag agaattagaa caacttcgag ttggcagcgg
     6001 cgttgaaaca aaagttaatt tggcaggaga aataataatc gctcaagatg cttcattcaa
     6061 agaagctcta attgatgtac tattgactaa atcaaaaaat caagaaatca aattttcatt
     6121 aaaaacaaaa gatgaagagt taattaatat tttttattct aaggataaaa atgaatgtac
     6181 cttaactcgt aacggcaaaa atgatccaag gtatggaacc attcagatgt gtgatagatt
     6241 atcacttcga gtgtttatcg ataaaagttc ggcagagatt tttattaatg atggcgagct
     6301 tgtttttaca gagcgttatt atacagaaaa tcaaccaagt atgcatttga gtgttagtga
     6361 agaagctttt gtctcgagta caatttatca tctaaaaaaa gatgcaattt cttacgagta
     6421 aggaggtttt atgatgttaa ttggtagcat agaagcaggt gggactaaat ttgtatgtgc
     6481 cgtgggagac gaggattacc gagtacaaga ccaaataatt tgtccaacga ccacccccag
     6541 ggaaacattg aaaaaaactg ttgactattt taaacagttt gatataaatg cattaggtat
     6601 tgcatcattt ggtccaatag aaattcgaaa aaattcacca aaatcaggat atattacgtc
     6661 tacaccaaag gtgggatggc gaaatgttaa tgtgttaggt tatttacaag atgagctgaa
     6721 tgttccaatg tcttggacaa cggatgtgaa cggatcagct tatggagagt acatcatgtc
     6781 cactttatcg aatgaaaaaa taaattcatt agtttattac accgttggta ctggcgttgg
     6841 tggtggagct gttgttgacg gaaaactatt aggatcgttt ggtcattcag aaatgggcca
     6901 tacgtttgta aaacgccatc ctgatgatat tgattttaaa ggaatttgtc cttatcatgg
     6961 tgattgttta gaaggcttag ttgcgggacc aacgtttgaa gcacgttttg gaaaaaaggg
     7021 tgaagacgtt tctttaacgg atcctatttg ggatgtaatg gcctattatc tagcacaagc
     7081 tgtgattcaa acaacattga ttatacgacc ggataaaata gtatttggtg gaagcgtagt
     7141 aaatgaaaaa ttgttagaaa aaattcgtgt taatgttaat gaattattgg atgattacgt
     7201 ggaaatacca ccgttaagta aatatattac gcgtccttta attaaaaata atggatcggc
     7261 aacactagga aactttgctt tgggcttgaa tcaattacaa gaataaaaca tagaaaaaat
     7321 atcgattatc aggtatagta gttctataga gttctttttt tcgatagtta attattgcat
     7381 tatttaggag agacaagaga atgcgcccaa aattagaaga tgttgccaaa agagcaaatg
     7441 tatcaaaaac aactgtttca cgtgtattaa acaatcgtgg ctatttaagt caaaaaacga
     7501 ttgataatgt ttataaggct atagaagaac tcaattatca accgaacgtt gtagcgcgtc
     7561 aattatttca aaagaaaact aacattgtcg ggttactatt tcctacagta gccaatcctt
     7621 tttttagtga actcgttgag gcattggaaa aaaagttata tgaaattggt tataaggtat
     7681 taataggcaa ttcgatgaat aataaagaaa aggaaacaaa ttatttaaat cagttattgt
     7741 ctgatcaagt agatggttta attgttggta cacataacca agggattcag gaatataaat
     7801 atcagaattt accgattgtt gcaattgatc gagtgatgaa tgaagatatt ccagttgttg
     7861 aatccgataa ctacaatggt ggaaaacttg caaccaagtt attaattgca caaggtgcaa
     7921 aaaatattat tcatacaaac ggtccaattg acttacagac tccggctaat cgtcggagac
     7981 tagcctatga agatactatg aaagcatatc agttaattcc acgaactgtg acattagatt
     8041 tcaatatttc ttatgtaaag aaaaaacaga tttttttcca aatgtttgag gattatccta
     8101 aaatagatgg tatttttgct tcaaatgaca tcgatgcggc cctaatatta caagtagcaa
     8161 aagaaaaggg gttaaatgta ccagctgatt tattagtagt cggatatgat ggtactttaa
     8221 tgactcggag tatattacca gatctgacaa cagtaataca accaattaat gatatagcag
     8281 acacagctgt tgctattttg atgaaacgaa taaataaaga agaaacaaaa aaggaatata
     8341 ttttacctgt cacattatgg atggggaaga catcagtaaa gaaaaaggac aattaaattt
     8401 ataaagacta tcaatgataa tttcattatt aattcgtgat ataaaaagtt gttaaatctc
     8461 ttataataga ctatgataca aaatcatgct tttaatctgt gtagatgttg ccaaatattt
     8521 gaaagacata gaacttgtca gaaaaacctt aacaattcct caatgggcgg ataagctagg
     8581 caaaagggct ggcataaact tttcagtcct tctaacagaa tcaattgcag ataaagcaga
     8641 taacattctt ctttctggaa gaaataatta gaactcaaaa gggtcagaaa cttaaaagtt
     8701 tttgatcctt tattttcaag tctgatttga cagattttcg gcagctcatt tatttatggt
     8761 cagaaatagt tgctgaaatt ttaatctatt agcaaatagt ccagaacgta tgaagaggat
     8821 tcggatatca tttgtcatta acaccataaa tatttataaa attcgtgttc acctacaatt
     8881 aaatttaata tataatattc gttgtaaggg cttataattt tttgttaatg aacaagatat
     8941 gtaattgata gtgtcaatag ctattcaaaa atgtaggctt ttggcccata aataacaagg
     9001 ttacttatat gataactttt tttctttata atctttaatc cgatagagct ttccagtagt
     9061 ttgaataatc tt
//
LOCUS       DAFFSV010000112         8808 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFFSV010000112 DAFFSV010000000
VERSION     DAFFSV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.112"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..294
                     /locus_tag="LR110_002322"
     CDS             <1..294
                     /locus_tag="LR110_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823267.1"
                     /translation="YTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVR
                     TRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(391..825)
                     /locus_tag="LR110_002323"
     CDS             complement(391..825)
                     /locus_tag="LR110_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4823268.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1057..1767)
                     /locus_tag="LR110_002324"
     CDS             complement(1057..1767)
                     /locus_tag="LR110_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL4823269.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1757..2617)
                     /locus_tag="LR110_002325"
     CDS             complement(1757..2617)
                     /locus_tag="LR110_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBL4823270.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2622..3260)
                     /locus_tag="LR110_002326"
     CDS             complement(2622..3260)
                     /locus_tag="LR110_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBL4823271.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3275..3574)
                     /locus_tag="LR110_002327"
     CDS             complement(3275..3574)
                     /locus_tag="LR110_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4823272.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3617..5101)
                     /locus_tag="LR110_002328"
     CDS             complement(3617..5101)
                     /locus_tag="LR110_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL4823273.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5121..5582)
                     /locus_tag="LR110_002329"
     CDS             complement(5121..5582)
                     /locus_tag="LR110_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4823274.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5600..6667)
                     /gene="ulaG"
                     /locus_tag="LR110_002330"
     CDS             complement(5600..6667)
                     /gene="ulaG"
                     /locus_tag="LR110_002330"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBL4823275.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6885..7655
                     /locus_tag="LR110_002331"
     CDS             6885..7655
                     /locus_tag="LR110_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL4823276.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7928..8755)
                     /locus_tag="LR110_002332"
     CDS             complement(7928..8755)
                     /locus_tag="LR110_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4823277.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2666 a         1858 c         1381 g         2903 t
ORIGIN      
        1 tatactgttg aatttgacag tgaactactt aagcaatttc cacaagaaga acatttcatg
       61 tataaagaag caatttccca caaagtgaca gagtatttgg agcataattt ttttcgtata
      121 aagccagtgc gtactcgttc ggcaaaagga atatacgaga tgaaagtcaa aattaataaa
      181 aaaaattatc gcatagcatt tgctttaaaa aaaaatcatg tgttcatttt ttatattagt
      241 tcgaatttgc aaaaaattgt atttgataaa gaggtgagta aaaaaataaa atagctgagc
      301 attggaggat agattgagtg atactaagtc tatcctcttg tttttctaaa gaactaagct
      361 agaatttttt cttttgtacg ttattatatg ttatttgtca ttaccaaaaa gcaccttttt
      421 tcttctagta tctatgacat aagaaatacc acccatttcc tttacttgaa caaatcctac
      481 tgattcataa aatttttttg ttttctcagt atcatctgtc aataattcga tttggactgg
      541 tccataacta gtgatcttct ccaaagcttc attcaaaagc actttgccta ttccttggcg
      601 ctgatacctc ggatgaatga ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat
      661 ccaacgaacg attccgataa tcttcccttc cccattacgc tttatcagag attcagattg
      721 ttcaaatgct ctagctaacc ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt
      781 atataattca gaaatctcat cgatcataag ggaatcaata ttcatttttt tcttcctttc
      841 ttatacaatt ctccctctaa taatacagtt ctttgactat ttaatctatc acatataact
      901 ctcctgtaaa gattctctcg attccttaaa attcctcttt ccttttagaa tgcatacttt
      961 ttgataaata aaaaggttgt gataaatttt cttcacaacc tctgtttctc tctatcaagt
     1021 gttatctttt aattttctac ccttacccta gcatctctat tgcccgtagt atgcatactt
     1081 tccatgtttg cgaaaataat gtttatctag taaatattgc gggatttcac atatattggg
     1141 gttgatcagc tcgttttctt tggccatcag acaaatttca tctaagatca aactgttttc
     1201 tactgctttc ataggtgaat ccccccatgt gaacggtcca tgtccataga caagtactcc
     1261 agggacttcg tttggatcta attttctttc cttaaacgtc tcgacaatca cattccctgt
     1321 atgaacttcg taagcttctc ttacttcttc tttcgttaat tggcgtgtac aaggaacctt
     1381 accataaaaa gcatcagcat gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc
     1441 ccacatgacg gagtgcgtgg aatgagtatg agtaatagca gttatatctt caaaagtttg
     1501 atacaaaaca acatgtgtgg ggagatctga agatggtttt aagctatctt cttctattat
     1561 atttcccgac aaatctgtta ccaccatttg atctgcttgc atgcattcat atctgacacc
     1621 gcttggctta atgacgatga ctcctagctc acggtccact tcactcacat tgccccaagt
     1681 caatttgact aatccgtatc gcggcaatgc cagatttgct tcaaggacac gttgcttcat
     1741 tgctgcaatt ttttcttcac aagacatcat agccagcctc cttcaattta gaacttaagt
     1801 agtcttttgc ctgctggatt tctcttcgaa agtcttggct tttttcactc cacatttcaa
     1861 tcagaaatgt tccatcataa tctagacgtt tcaaatgtct taataatcca agaaaatcaa
     1921 cacaaccgct accaaaagga acatcacgaa actggcctga atattctttt gttacagggt
     1981 aagtatcttt taagtggata gcagtgatat aatcgatccc tttctcaagt tctcgagccg
     2041 gattattttc cggccatgcg gacaaattgc ctaggtctgg atatacttgt agaaaaggtg
     2101 aatgaatctg ttctttgatt tctaaaaatt ttgaaatgga gttcataaat ggatcatcca
     2161 tgatttccac ggaaagaatg atgccatatt tagaagcttc ctttacgctt tcttttattc
     2221 cttcgataaa gtcttctctg gaattcattg atttctcttc ataataaaca tcgtatccag
     2281 ctatttggat tactttgacg cttagattat gcgccaaatg gattgctttt ttcattagtt
     2341 gtttcgctgc ttttttcttt tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac
     2401 ttaaacaaat cgaatggata cgtacaccgg agctgaacat ctcttctctc agctcagcta
     2461 tcttttcttc agaccaatct aatcgtgcta accgctcatc cgtctcatca atcgacatct
     2521 cgataaaatc gaagtccatt tcttttacaa gggaaaaacg ttctttccat gaaatatctt
     2581 tgggtaacgc tttttcataa atcccaattt gagccatttc cttaccccca gatacgaatg
     2641 atctcatctt tgaagttttg tgcggccttc atcggatcat ttgctgctgt aataccacga
     2701 ccagtgataa aggtatacac atcgatccct tcaaataagt gtaatgtgtg tggatttaat
     2761 ccaccagtta cagaaacatt aaagcctagt tcaatcagtt ttttgatctt cgataaatct
     2821 ttttcacccc aagtttcacc agataacagc gcatcacggc tttgatggta gattacctgc
     2881 cggataccga tgttatgcca atccatggct tgttcaaaag tccaattgcc gtagagttct
     2941 acttgcaatt cacctatttc tttttgtgct gcctccatag ttggaatcgt tgcacaacaa
     3001 atgactgtca tccagtctgc tcctgctttt gcaacattac tcgccactgt tcctccagcg
     3061 tctgcacatt tcgtatcagc gactagtttt ttatctggga acatactgcg aatacagcga
     3121 atcgcttttt gtccttcctg taagcaaaga attgtgccca cttcaataat atctactaca
     3181 tcaccaactt tcataacatc tcctaatgca tgttctaagt cattgtgatc taacgcaatt
     3241 tgcaaattcg gcctgctcat cttagttcct tcctttactt gtttttttgg taccactttg
     3301 tttcagcaaa tttttcactg atttctttga ctgacatcac atttttgata ccgataactg
     3361 taactccttt tttctctgcc tgagtaaaca tattcaaaaa attgactggc gtaaaaacta
     3421 catcgtattg gccagctgaa ctttttccct ctgaaatggc acaatgatgg attttggtaa
     3481 ttggtacgtt tagttctttc attgcttttt ccacacttct catcatcatc aagcttgttc
     3541 ctgatccatt tgcacacgat actaaaattc tcatcttaat ccctccatta tgagtgttgt
     3601 ctaatcagat tttcttttat actctctgct tttctgcata tgtgtcatag tcttctacaa
     3661 tcaaaaaata accatcagga tttttccgat attgtaactg cggaatagcc aataaaccaa
     3721 tgacaactac ggcaattcca gcataaccta ggactttcat gattactgta aacaatggcc
     3781 aagctgttgc ccagtcgaac atcccgatat aaccaccgta tgttgacatc ccaatccatg
     3841 tagcgaataa tgctgaaccg cctacttgta tgattcctga aagaaacggg aacaggcaag
     3901 cggctttgaa tccacctcgg ttattggcat aaactgctat aacggcatta tcaaagaaca
     3961 acggaatgaa accggctatg acgatcgttg gtgatttcaa taaaatcagt aaaccaatca
     4021 tcaaaaactg tcccaaagca ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa
     4081 agccaaaagt agcagctacg tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga
     4141 ttccttggaa agattgagtt agttcatcga cgaatgtccg aacacctaat tgcaagatcg
     4201 ccagatatac cgcaaaattt aaagaagtcg tcataatgta gaataagaaa ctctgccctt
     4261 cttgcatgaa ttgcgcttga atcaaataat ctttgcctag tacaatcaga atgatcccaa
     4321 agaaaaatag catgagtaac gaagtagcaa ccatattctc attgaaaatc gacaagaatc
     4381 ctggtaattt gatatcttca atttttttgt cttgcttgat tgatctattt ttcttttcat
     4441 cccgcttttt catccactca gctaattttg caaatataaa gattccgaac atttgctgat
     4501 gtgccactgc aaatccggca ccttctgtca attcctgcgt gattcctact gttaagttag
     4561 aagctactgt ccaataacaa cctaaaatca gtcccataaa aatcaatgct tgtatcctac
     4621 ctagatcagg aaagcaaaac agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa
     4681 cgtttccggt agtgaaaacg gaacgcagtt tcgtatattt ctgaaaacgg actaataaga
     4741 tatttaaaac aaatgcaatc agtaataaaa tcatcacatc tccaaaggta cggccaaatg
     4801 tctccattaa tccagcatcc acagcatttt gtccaaaata aggatctgta accatggcat
     4861 ccaaattgaa ccgttccttt agtccaacca agattggccg aaaattattg accaaaccac
     4921 cagagccaac agtcaaaata aagtaaccga ccgttgcctt taagaatcca gcgatgcatt
     4981 catgaagcgg gcgtttcagc aaaatatagc ctaataacac gataaaacca atcaggtatg
     5041 ctggctgcgt caagatattt gttgcaaaat acgtccaaat gcttaacaaa aattccccca
     5101 tcttttttct cctctatcaa ttaattatat tctgccatca caacttggta atcatcgatg
     5161 gagttcgtag agatgagacg ctcaaccaat ccatctgtca ttaataattc ggataatttt
     5221 tggatatttt ctagatgttc ttctggatct tttgctgcga gtgtaaaaaa caaactagcc
     5281 gttttctcct cattctcccc ttcactttca aaacggattg gctgtttcaa cttggtaaat
     5341 gaaatagctg ttccaaaaac accatcactt ttttctgaag aatgcggcat tgctacttga
     5401 ggtgcgatca caatataagg accgtatttt tctacacaat ggatgatctc ctccacatac
     5461 gtttcatcaa taatttcctt atcgatcaat acaagacagc tttcggcgat agcttcacgc
     5521 caatccgtga tttctttatc tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc
     5581 aagaaactcc ccttctttct tataaaaacg acggaaacgg tgtatcattt tcaatcgaga
     5641 agacatcttc aaatcctcga taaaaattaa actcaagatc atctttgttt gtcggataga
     5701 tgaatttccc gcctacttgc cagatataag gtttaaatcc atacttcagg cgatcctttt
     5761 tgaatttcca aatttcagtg atctcttttg gatcagccat aaaattagac caaatatcgt
     5821 agtggacagg aatcactact tttgcattca atgactccgc catgcgcagc atatcaacag
     5881 aagtaacttt gtctgttatc cctcgtgggt tttcaccata ggcccctaaa cagacatcga
     5941 ttttgtgttc attaccatgt ttggcaaata aattggagta atgtgaatct cctgcatgat
     6001 aaagattccc tcctgatgtt tcgaataaat agtttactgc aatttgatcc atctcctgag
     6061 gcatttttcc ttttaatacc tcttctggat cttcacaagt gaccaacgct gtccgatcaa
     6121 acgcttctaa agctactaca ttgatgtctt tgattttgac tgcatcacct ggctttactg
     6181 taatcgtttt ttcttcagga attccccatt tttgccacgt gcgaaccact tcttttggcc
     6241 caatgaatgt tgcttcagga caattttgat gaactgctgc ggctgtatgg atatctagat
     6301 gatcggaatg gatatgtgta acgattaaag cgtctacatt ttttatcgca aaaggatcga
     6361 ttacgaaagg ctgagtacgt aaattaggct gcatattttg aacgccactc atacgcatca
     6421 tctgatggcc ttttttcatc atgccattcc catgactacg ttttcccgtc ccacaccata
     6481 gatcacataa aatattcgtt ccttcatgac ttttcagcca aattcccgta cagcccagcc
     6541 accacatagc aaaagttcca ggatccacta cttctgtttc gatttcttca ttcaagtatg
     6601 tgccccactc aggaaatgtt tctactaacc acttttcttt tgttacttca ttgacagttt
     6661 tactcatttg cttgctcccc ccatatcgtt gttccaactt acaagtaaat attaatgtgt
     6721 atcagcatcc atgaaaaccc ttttatatga ccgtttgttt tttatatgat tttctttcag
     6781 taatcaatga ttgtttcagt ataataaaga cagatatgaa tgtttaaatg aacaactagt
     6841 caaataaatc catggatcaa gatgtccctc aggaggcaaa agtcatgaaa aactcacttg
     6901 tcaatattga aaaacgtcaa aaagaaattt tggaactgct tcagcagact gaacaactat
     6961 caactttcga attagctgaa tcccttgatg tatctattag tacgatacga agagatttaa
     7021 atttattaga agaaaaaaac gagatcatac gcaattacgg ttattgttct tataattaca
     7081 aaaaccagac tgattttgat caatctggtc ctgaaaggat taaacaagcg attgccagag
     7141 aagctagtaa atacattaac gattatgata cgctgtttat caattctagc tcgactgcgt
     7201 tgaaaacact ggattttctt caatctaacc atttgacagt cattacaaac aacgtgaaaa
     7261 tcaattactc tccgcacaaa gataattgta gttatatttt aactggtgga gagatgcgct
     7321 ttccaaaaga agcactagtg ggcgacatcg cattaaatac gatttcttca gtcaatgcag
     7381 acatttgtat cattggctgt agcggagtcg atgtagtaca tggggtcaca acaaagatat
     7441 taaatgaatc aacgatcaac gaaatgatgg tcaacaagac aatcaaatgt aagattttag
     7501 tcgctgatca tcgaaaaatc ggactgacat caaagttcaa gattgctgat atttcagtct
     7561 ttgattattt gatcactgat aaattttgtt ctgaaaaagt attagaggag atcagaaata
     7621 cggggataaa aaccattcaa gtagattcat tttaagaacc aggacgtaaa tatgtaaata
     7681 ggattatcaa aaaaatgaat aacaaattta tcgataaatg aaaaacacgc tacgctcaaa
     7741 ttgataaagg aatcccaaga tgtatccatc caatcgatac catcataatc tattgcttct
     7801 tttataaccg ttatatgttg cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg
     7861 acaaaagtca cacctccttt tttcgaataa acggtattca aatcagaaca ctgatttcac
     7921 aaccacttta tttcaggtcg ttttcaatca cctttaccgc gtaatcattt ccgttgaaac
     7981 gcgctatttt ttttgcgtcc atatatcttt ctaaataatc atcttctcct aattgcttat
     8041 ggcaagaagc aatttcataa agcaagtatt ccaatgcata agtcgattta tgtttcttat
     8101 tatactcaat acccttctca cataattgga ttgctttatc ataatcctcg attttacggt
     8161 aataggtggc gatatcataa tatacaatgc agaccatcag attatcaaaa tcagtctcac
     8221 tcaatatttt aagtgtacgc tccacataaa ctttcgcaaa atctaattct ccttttgaca
     8281 agtagatcat tgccacgttt gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt
     8341 gttccaacac ttgattaaaa taaaataatg ctgcatctgg ttttttcgca taaagatgat
     8401 caagcagtcc ttgataaaat aaaatttgat ttaaaagatc agaattactt actttttcct
     8461 gatcgatcgt ttgtaaaatt ttataagatt cttggtattt tgatacagaa ttcaatgatt
     8521 gaatctcatt tatttttttc ctgattttgt cttcttctgt ttctactaat atatcggaga
     8581 tctcgacatt cagctttttg caaagcgcac ttaagatatt gatgcctggc gacatattct
     8641 tgttttcgat attgctgaca gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg
     8701 ttaacccctt tttctttcgg tattctttta gtaatgctcc atctacttta atcaaaacaa
     8761 tttccttccc tttaaaaaat tatttctcct ttatatcata cacaaaaa
//
LOCUS       DAFFSV010000113         8797 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFFSV010000113 DAFFSV010000000
VERSION     DAFFSV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.113"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            3..1457
                     /locus_tag="LR110_002333"
     CDS             3..1457
                     /locus_tag="LR110_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBL4823278.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPASMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1533..2693
                     /locus_tag="LR110_002334"
     CDS             1533..2693
                     /locus_tag="LR110_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBL4823279.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2806..3813
                     /locus_tag="LR110_002335"
     CDS             2806..3813
                     /locus_tag="LR110_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL4823280.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3837..4862
                     /gene="iolG"
                     /locus_tag="LR110_002336"
     CDS             3837..4862
                     /gene="iolG"
                     /locus_tag="LR110_002336"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HBL4823281.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4879..5769
                     /gene="iolE"
                     /locus_tag="LR110_002337"
     CDS             4879..5769
                     /gene="iolE"
                     /locus_tag="LR110_002337"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HBL4823282.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5792..7387
                     /locus_tag="LR110_002338"
     CDS             5792..7387
                     /locus_tag="LR110_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBL4823283.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7403..7717
                     /locus_tag="LR110_002339"
     CDS             7403..7717
                     /locus_tag="LR110_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL4823284.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            8178..>8797
                     /locus_tag="LR110_002340"
     CDS             8178..>8797
                     /locus_tag="LR110_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL4823285.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILL"
BASE COUNT         2675 a         1607 c         2075 g         2440 t
ORIGIN      
        1 aaatgacaag atttttaaga aattatattg atggtcgttg ggtcgctagt cgctcaacag
       61 agtttgaaga agtaatgaat ccggctacag aagaagcgct ggcgaatgta ccgttgtcta
      121 gcaaagcaga gacagccgaa gcagttaaag ctgccgatgc tgcgtttcaa acttggaaaa
      181 aagttcctgt tcctgttcgc ggacgttatc tgttcaagct gcatcagctt ttaacagatc
      241 ataaaagtga attggcgcgt ttgatcacgc tggaaaatgg gaaatcattg tccgagtcac
      301 tgggagaagt tcaacgagga atcgaaaatg tggagcacgc agcgggaatc accaatttaa
      361 tgatgggaga ttctttatca acagcggcga ctgatattga gatcacgaat tatcgttacc
      421 caatcggagt aatcggaggg atcacaccgt ttaattttcc aatgatggtg cccttctgga
      481 tcttcccgat ggctctggcg gcaggaaata cggtggtgct gaagccttca gaaaaaacac
      541 cattgttgat ggagaaaatt gttgagctgg ttgaaaaagc aggctttcct agcggtgttt
      601 ttaatgttgt ctatggtgcc cacgatgttg tcaacacatt gctgcaagat ccactggtaa
      661 aaggcatcag ctttgtagga tcaaaaaatg tcggtgaata cgtttaccgt gaaggaacaa
      721 aaaatctaaa acgggtacaa gcgttgactg gagcgaaaaa tcatgttgtt gtgctgaaag
      781 atgccaatat tgatcgggca gtcaaggacc ttattggcgg agcttttggt tctgctggtg
      841 aacgctgcat ggcagcctcc gtcattgttt tggaagagga aatcgcggat cagtttgttg
      901 aaaaatttaa ggctgcggca aaagcgatca agatcggcaa tggcttggac gaaggggtct
      961 ttttaggacc agtgattcga aaagatgcac agcagcggac ctttgactat atccaaaaag
     1021 gggtcgatga aggagcgaaa ctggtactag atggtcgcga aaacattcca gaaaaaggct
     1081 attttgttgg accaacgatt tttgataatg tcacgatgga tatgaagatc tggcaggatg
     1141 agatttttgc cccggtcgca tcaatcgtgc gggtaaaaga tttgatggca gccgttgagc
     1201 tgacgaatga atctgaattt gcgaatggtt cgtgtctgta taccaacaac gcatcagcga
     1261 ttcgctactt cagagaaaat gttgatgctg ggatgctggg ggtcaatctt ggggtgcctg
     1321 catcgatggc ttggtttgcc ttttcaggct ggaagcactc tttctttggt gatttgcatg
     1381 ccaatggcaa agacagtgtt gagttttata cccatcggaa agttgttaca gcacgttatg
     1441 cgcaaagtga gatttaaaga tttgcttcga agctctgtta aagagcttcg aggattctgt
     1501 tataggctat aagccagaga gaggtttttt ctgtgaatat tcaactgaaa atgcctgcca
     1561 atttaagaat tggcggcgga acaatccaat ttcttatgga agaatcagaa caactaggca
     1621 acaagccgtt aatcattagt gatacggtta tggaacagac cgggcatctt caaaagatca
     1681 aggagctgtt gggaaaaaca gatgatcaat tagcggagta tacagacatt caagccgaac
     1741 cgacgcaaga aaatgtttat gatgcgctga agctttttca gaagcatcaa tgtgatcata
     1801 tcattgcttt tggtggcggc agctgtctgg atacagccaa ggtggtagcc gttttggctg
     1861 ctaatcaatt aagtctcgaa gcgattcatg caagaaattt tgatcagctg caagcgggca
     1921 ttccgctgat tgcagtgtca acaactgctg gaactggttc ggaagtaacc gatgtggcag
     1981 ttattaccaa tacaaaaaca gcagtcaaaa tgatgatgaa ggatctcgtt ttcatgccac
     2041 agatctcaat cgttgactct gattttacga aaactgtacc accaaaagta accgcggcaa
     2101 cgggagtcga tgcgctatgt cacgctttgg aatcttatgt ttcagtcaaa gccaatgaag
     2161 taacgcaact attttcactg gaagcgatga agaaaatttt gaacacgctt tctcaggcat
     2221 atgaaaatag cgacgacact gaagccagag aacaaatgag cttagcttca acggaggcgg
     2281 ggattgcctt ttcgaatgcg tcagtcacat tgattcatgg aatgagtcga ccggtggggg
     2341 cactttttca cattcctcat gggatatcaa atgcgatgtt attgccggtc ttttttgaat
     2401 tctcaaagga tgctattcag actgagttag cgacgatcgc cagatttatt tatccggcag
     2461 ccgaagattt atcgctggaa gaggctgcgg attatctgat taaaaaaatc caagagctct
     2521 gtgagcagct gtcgatccca acgattcgag attatggtat tgatcagcaa gcgtttatgg
     2581 cagccttaga caaaatggcc gcggatgcgt tgaccagcgg aagtccgcaa aataataaaa
     2641 aggtgcctag tcatagtcaa attaaggaat tatacgtaca agcttatggg tagtaaaagg
     2701 cggagcaaag agagggctct accttttaag aataaaagct acacaggaat aattttgtaa
     2761 gcgcatacta tgtgtgctta aacaacagaa cagggggata agtaattgag agtaaaagta
     2821 ggcgttatcg gaacaggtgc aatcggcatc gagcatatcg acaggatcaa tcagaaaaca
     2881 caaggggctt atgtgacagc agtctcagat atcaatgtaa aagctgctaa agaggttgcc
     2941 aaacgtgtcg atgctaaatt ctttgaaacc ggagaagcat tgatcgcttc tgaagatgtg
     3001 gatgtcgttg ttgtgacttc ttgggacccg acccatgagc agtatgtgtt agaagcaatc
     3061 aagcatggga aatatgtttt ctgcgaaaag ccattagcga tggatgcagc aggctgccgc
     3121 cggatcgttg atgcggaagt tgcttatggg aagaaattgg ttcaggttgg cttcatgcgc
     3181 cgctatgacc gcggctacat tgaactgaaa gaagcaatcg aatcgaagca atacggagaa
     3241 gcattaatga tgcattgtgc ccatagaaat cctagcgcag atgaaaacta cacaacaccg
     3301 atggcgatca gcaacaccgc gattcatgag attgatgtgc ttcgctggtt gctaaatgaa
     3361 gattatgaat ctgttcaaat gatcttgccg aaaaaaacca gcaactccca tgccgatttg
     3421 catgatccgc agctgctgat ctttcaaaca aaatccggtg taacgattga tttggaaatc
     3481 tttgtgaatt gccgctttgg ctacgatatt aaatgtgatg tggttttcga aacggcagaa
     3541 gtaggaatga cggacccagc ctatacacga attaaatcgg aagaaaaaaa ttacacacct
     3601 gtttcaccag attggcagac ccgctttttg gatgcctatg attatgaatt caagctgtgg
     3661 atcgattcga tcaaaaatga tgcggttgtc ggtcctaccg cttgggatgg gtatgtagca
     3721 gcgatcacca tgatggcttg tcatgagtct cgtgaaacaa aaacaaaagt aatgattcaa
     3781 atggatgaac gaccagcact atacaatgag taattctcag aaaacggagg tatttcatga
     3841 acaacattag agtaggcatc gtcggtttag gaagattagg aaaagtacat gcacaaaatt
     3901 tagcaacagg tgtcagttgt tgtgagctta cggctgcgtg cagtttagtt gaaacagaat
     3961 tagcattcgc acagaagaaa ttaggcgtca agcatacttt ttccagctat gaagaaatgg
     4021 tcaaaagttc cgtgatcgat gcagtcttta ttgtttcacc atcaggcttt cactgtgacc
     4081 aagtccgctt ggcgatggaa aatggcaagc atgtctttac tgaaaaacca ttaggccttg
     4141 aagttgaaga tattaaaaag acccaaaaag tcatcgaaga gcatcctgaa caggtcttca
     4201 tgttaggctt catgcgccgc tatgatgagt cgtatcagta tgcgaagaaa atggtagaag
     4261 atggcgaaat cggcgaaatc acactgattc gttgttatgg gatcgatccg agcgaaggct
     4321 tggaaagctt tgtcaaattt gccatgaata acgacagcgg cggcttgttc ttagatatgg
     4381 cgatccatga tattgatttg gttcgctggt ttaccggcca agaagtcgat caagtgtggg
     4441 cgattgggaa aaatgcggcg tatccagaat tggatcaggc tggtgagctt gaaactggag
     4501 ccgcaatgat gagcctgagc gataaaacaa tagctttact agttgccggc agaaacgcag
     4561 cccatggcta tcatgtcgaa acagagatca tgggaaccaa aggaatgctg cgggtagcgc
     4621 aagcgcctga gaaaaatctt gtgacgatca tgaacgatca agggatcatc cgtccatgtt
     4681 cacaaaactt cccagaacga ttcagagaag ccttcattcg tgaagcccaa gaatttgtct
     4741 catgtattca agaaaaacgt caaccagcgg ttaatgcaga agatggattg caatcaacta
     4801 agatcgcgtt ggcttgtcaa gaatcattcg atacgaagag tttagtacaa ctaaaaaaat
     4861 aagcaggagt gtgagaatat gaccaaacaa attcaattag ccatcgcacc aatcgcgtgg
     4921 accaatgatg atatgcccga gcttggtagt gaaaatacct ttgaacaatg tatcagtgaa
     4981 atggcccttg ctgggttcac cggaacagag attggcaaca aatatccaaa agatccagag
     5041 gtgttggaac aatatttgaa tattcgcggc ttaaaggttg ccagtgcatg gttcagtgcg
     5101 tttctgacaa ctaaaccttt tgaagaaact gccgaagcct tcaaagcaca tcgggatttc
     5161 ttacatgcga tgggcgctaa agtcatcgta gtagctgagc agggtcatag tatccaaggg
     5221 attatgacag cgccagtctt tgatgataaa ccagttttca ctgatgaaga gtggaaaaaa
     5281 ctggcggaag gattagaaaa attaggggat ttggcccacg aaaaggatat ggagatcgtt
     5341 taccatcatc acatgggtac cggcgttcaa acgacggaag aaatcgatcg tttaatggaa
     5401 atgactgatc cagacaaagt taaattattg ttcgatacgg gacatttggt cttttctggt
     5461 gaaaatccaa tcgcggttta caaccgtcat caagatcgta tcaaacacat ccatttcaaa
     5521 gatattcgtc aagaaacggc tgaagaagta gaaaaggaaa aagacagctt cttaaacggc
     5581 gtgaagaaag gcgtcttcac ggttcctggt gatgggatga ttgatttcac tccaatctgg
     5641 acagcaatcc atgccagcga ctatgaaggc tggatcgtag ttgaagcaga acaggacccg
     5701 gctaaagcca atccatttga atatgcagtc aaagccagaa gctacattcg agaggtcacc
     5761 ggtctttaat ctattgaaaa ggagctagac gatgaatctt ttttctataa tcagtttcct
     5821 agtgattgtc atctgtgtat ggttatttgc ctattctaga tccagaagtg tcgatacttc
     5881 tggatcagaa gggttcttca tgggagggcg gagcttaact gctttgccaa tcgccggcac
     5941 gatcatcatg acgaacctgt ccaccgaaca aatagtcgga caaaatggtc aaagttatca
     6001 tgcagggatg gaagtaatgg cctgggaagt aactgctacg attgcgatcg tagctttggc
     6061 tgtgatattc ttgccaaaat attttaaata cggcatcaat acaatctcag atttcatcga
     6121 gattcgttat gatacggtta ccaaacgaat tatttcgatc ttatttattg tgacctatat
     6181 gacttctttc ttacccgtgg ttctttattc tggatcgctg gtgttcaata agatcttcca
     6241 tattgatgag ctgctgggcg tccagccgat cgtcgcgatc ataattgtag cgatggcaat
     6301 tggtattgtc ggcatcctct atctgctgat cggcggcttg tcgttaagtg ccaatagtga
     6361 ctccatttat ggagtcggtc tgctggcttg tggtctgctg gtcccaattt taggcttaat
     6421 gaagcttggt gacggcagct ttgttggcgg gatcgaacac gttgtggtaa acacgccatg
     6481 gctgcttaac tctgtgggat cgatcgactc ggctgttgtt ccatggccga cactatttac
     6541 agggatgctg tttaacaatc tgtatttctg gtgtaccaat caaatgatcg tccaaaaagc
     6601 attgtccggc aagaatttag ctgaggcaca aaagggcgct ttcctagtcg gcgtcttcaa
     6661 aatcttaggt gcgttattcc ttgtctttcc agggatcgtc gctcgcaata tttttggcga
     6721 tagtttaatg tcaaatccag ataacgcgta tcctttttta gtcagtgaag tattgcctaa
     6781 aatgatcttt ggtattttcg ccgctgttat ctttggcgcg atcttatcat catttgcggg
     6841 agctttgaac gcgacatcga ccttgttttc actggatttt tataaaccgc ttatcaataa
     6901 gaaagcaaat gataagcaaa tcgctcgtgc tggtaaaaca gtaacggtcg ttgtggggat
     6961 tatctccatt atcgtagcgc cattcatttc ttttgcaccg gctggattgt atcagttcgt
     7021 tcaagaattc aatggactat acaacatgcc gctgctcgtg atcattctct ttgcctttta
     7081 ttcaaaaaaa gcaactgctt ttggggcaaa aacgacgatt gccgtgcata tcgtcttgta
     7141 tgcactatca aaaatgcttt tccaaaatat ccatttcctt tatgttttga cggcattatt
     7201 cgttctggac attatcattt tgatggtggt ttccaaattg aaacctgatg gagaatttgt
     7261 gttgaactca ttcgatacaa aggtcgatat cgagccttgg aaatacacca aggtgactgc
     7321 tatagcattg gtagtcattg ttattttaac ttatgtggca ttttcaccaa ttggctttgc
     7381 aggctaaaaa caggagggta ttatgcataa ggatctattg attatttgcg agaccggaat
     7441 cagtgcgtcg ctgctcgtat caaaaatgct tgaaacgatc agagccaaaa atctatcgta
     7501 tgagatagat tatgcaacga tccgaaacgt tgaagaaaaa ctgaattata aagactatga
     7561 cgttttactt ttaacgccgc aggtcgcacg ttctgaggaa aaagtaaaag aaatcatcaa
     7621 aaaggaaaaa tgtaccgctc agatcgttta tatcaaacaa gaagattttc gttatatgaa
     7681 tattgaaaac atcattgcaa gcatcgaaga agattaacga gaatctttga ttattgagtg
     7741 tcgaggaggt ggtatatgaa aagatggcat gttcgccccg atcaccccaa tatgaagggc
     7801 ggacttatta gaaataccca atcttgtatt tattcaatag ggtaagcgga cctcaatagc
     7861 cagctcatcc ccaaaaagtc gaatggagac acttaaatga gtgtacctat tcgacttttt
     7921 gagactctaa gtcaaatcgt gttgttctca ttaaatcact gaaaggaaat gcttcggcgt
     7981 ttcctttttt ctttttgttt ttcttcgtgt tttgaaaatt acttactatt tactatacga
     8041 gtagtttcaa ataattttga catcctaatc ttcaacaaca gaaaaaaatc taaattataa
     8101 aatttagggt tgactaaaat ataatttttg tataccatat gatcgtgata atcattatca
     8161 tttagaagga gaaagttatg ttagaagtga aaaagcttaa tgtttgttat aacgattttt
     8221 tagcattgaa cgagatttct ttaaaaatac aagaaggtgc tttaacaggt attattggtc
     8281 caaatggtgc tggaaaatct acattgctta aagcaatgtt aaatattatc cctcatcagg
     8341 gagaggtctc cattaataat gaagatatca ataaaaaact ggtgaagatt gcatatgtag
     8401 agcaaaaagc tgatattgat tttacttttc caataaaagt aaaagaatgt gtatctatgg
     8461 gaacatatgc cggaatgaaa gtttttcaaa gaattaaaaa tgcagaatgg caaagagttt
     8521 caaaggcttt ggaaaaggtc gatatgggaa aatattctaa tcatcaaata ggagaattat
     8581 ctggaggaca atttcaacgt gtacttttag caagatgtct tgctcaaaat gcggatttca
     8641 tatttttaga tgaaccattt gtagggatag acttagttag tgaacgaata attatggata
     8701 cgttaaaaga attgaaacat cagggaaaaa cagtgctaat tgtgcatcat gatttaagca
     8761 aggtaaagaa gtattttgat aatattattc ttttgaa
//
LOCUS       DAFFSV010000114         8774 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFFSV010000114 DAFFSV010000000
VERSION     DAFFSV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.114"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            38..3265
                     /gene="fss3"
                     /locus_tag="LR110_002341"
     CDS             38..3265
                     /gene="fss3"
                     /locus_tag="LR110_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL4823286.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3377..3691
                     /locus_tag="LR110_002342"
     CDS             3377..3691
                     /locus_tag="LR110_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL4823287.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3704..4078
                     /locus_tag="LR110_002343"
     CDS             3704..4078
                     /locus_tag="LR110_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL4823288.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4079..4432
                     /locus_tag="LR110_002344"
     CDS             4079..4432
                     /locus_tag="LR110_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBL4823289.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            4501..5517
                     /locus_tag="LR110_002345"
     CDS             4501..5517
                     /locus_tag="LR110_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL4823290.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6111..7118
                     /locus_tag="LR110_002346"
                     /pseudo
     CDS             6111..7118
                     /locus_tag="LR110_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7321..8640)
                     /locus_tag="LR110_002347"
     CDS             complement(7321..8640)
                     /locus_tag="LR110_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBL4823291.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT         2948 a         1520 c         1866 g         2440 t
ORIGIN      
        1 ttattttttt gtgcctaatt tgaaaggaga acatttcatg tataaaaaac aaaaaaagaa
       61 attatgtgta ttaactattt tgagtttggc tgttcaatca attgtaggag caattgctcc
      121 tacgattgct tttgctgatg aaattaccca tccacaaacg gtaaccgtag aactagattt
      181 agcacatcaa tatgctgttg aaggaacttt tagtgatggt cgtccaatgt cagaagtcac
      241 tgttccgcat tatgcagtgt acaacggcgt taaacaagat attttctgtg ttgaaccagg
      301 agtcccgatt tacaatgagt tcactcctgg atatgagaag aacccgttac cagatatgtc
      361 cgataaagcc aaattagttt ccgttttatg gaaaaaagta ggtacagatg cagatactca
      421 tattgtggca cagaagatga tctggcaaga agttaatggg tatacccttc actcaataaa
      481 acgatcagat ggtagtgccg taaatatcgc agccattgag gcaaaaatca atcaagccat
      541 tgccgattat cagaagaaac caagcttcca taatagtacg gctaaaacag tattagggca
      601 atcgactact gtgacggata cgaataatct gaatttgtca gagttcgatg aggttgtgga
      661 aaataccgca aacattgatt atcgtgtgaa tggcaatcag ttgataatca gcccaaatgc
      721 caattcaaaa gaaagtggtg tgttgacgct taagaaatct gctggtaccg gaacacctgt
      781 cgcctataaa aaggctggcc aacagacttt aatggccggg gcgatcgata agccgaacac
      841 ttatacggtc aaaattgatg tggaaatgga gggttctttg aagattaaaa aagttgataa
      901 agaatcgggt gatattatac caggaacggt tttccattta gactttggaa aaactttacc
      961 cgcaaaagac gtgaccaccg ataaagaagg cattgcgacc ttagatgaga ttcctcatgg
     1021 tacaaaggta accattactg aaaaatcggt gccggcgcct tatacgattg ataccacacc
     1081 catgactgcc acaattaaag caggtgaaac catttcagtg acttcaaaaa atacgcgaga
     1141 aaaaggtcaa attattctgg ataaaaccgg aattgaaaca gggagtgatc tttggaacga
     1201 taattactct ttagcgggca atacgtttgc cattcgcaag gatagcccaa ctggtgaaat
     1261 cgtccaagaa atgaccacgg atgaaaaagg tcatgcagaa acaccaaaag agattgccaa
     1321 tgcgttggaa ttgggaacct actatgtgac tgaaaccaag gctagtcatg gctttgtgaa
     1381 tactttcaaa ccagtaaaag tcgaattgaa ttatgtcaat caaacagtgg ctcttgttac
     1441 cagtaacgta aaagggcaaa accaagaaat tactggtgaa accactttga caaaagaaga
     1501 caaagatacc ggtcatgaaa ctcaagggaa agctgagttt aaaagagctg aatacacctt
     1561 gtttactgtt aaggatggaa aagcggtcaa atggaccgag aactttaaac cagaattgtt
     1621 gaagggaacg aaagcttctg atgaaaccgt aactttgact ttagatgaaa agaatcaagt
     1681 tgccgttaaa cacttagcga ttaatgagta ttactggcaa gaaaccaaag cgcctgaagg
     1741 atataccttg gatgaaacaa agtatcctgt atccatcaaa aaggtggatg atagcgaaaa
     1801 aaatgctgtg attactcgag atgttgcggc aaaagaacaa gtgattcgct ttggctttga
     1861 tttctttaaa tttgctggat caactactgg cactgccgaa actggcttca atgatctaac
     1921 ctttaaagtg tcgccattgg aaggaaccaa tgaaatcacc ggtgccgaag atgaagcgac
     1981 cacagcttat aacgagcaat tagggtttga tggttatggc aagtttgaaa atcttcctta
     2041 tggggattat ttacttgaag aagtagaagc cccagaaggt tttcaaaaga ttacgccatt
     2101 agaaatccgt tctactttta aagaaaataa agaggattat accaagagtg attatatctt
     2161 tactattacc gaaaaagggc aaaagcaacc gattaaaacg gtaacggtcc cttatgggaa
     2221 tctaaccaat gagaatttct cagtcagttt gaaccgtttg atgctttatg atttgcctga
     2281 ggaagaagat agcttaactt ctcttgcgac ttggaaagaa ggagataaag aattgactac
     2341 tcttgattct accgagctag tcgataaatt gagctataat ctacatgaaa tcaaagaaga
     2401 ctggtatatc gtagctcaag ccatcgatgt agatgctaca aaagctgccc aagaaaaaga
     2461 taaaaaagcc aaaccggtgg cgattgcgga aacaaccgca actttagcga ataaagagaa
     2521 aacaggaact tgggagattc tgcataaatt aactgtcgaa caagttttag acaagaccat
     2581 tgtcttattc aactatgtgt atgaaagcaa agaagcctat gaagctggtg atgagccggt
     2641 agcgaaggat gctagcttga acaatcaagc gcaaactgtt aattgtatgg tggaacgtca
     2701 tgtttccatc caaaccaaag cgcacttaga agaaggttct cagaccttta ctcatggtga
     2761 cgtggtagat atgtttgatg atgtatccat tacccatgat gtactagatg gctcaaaaga
     2821 agcttttgaa acaattctgt acgctttatt tccagatggt acaagtaaag aaatttggaa
     2881 atctggcaag attgattacg aagtgaatga caaagaattt accaaaaccg tacttgcgga
     2941 aaaagtggat acaagtaaat atccagaagg tacaagtttt accttcaaag aaattaacta
     3001 tgacaaagat gggaatatca atggaaaaca caatgaagat ttgaaagaaa aatctcaaac
     3061 tttaacgccc aaaaaagttc cgacaacacc aagtacacca gaacaatctg aaatgccaac
     3121 tgttccaagt ggctctcaag aatctagtcc tacagtgaag gcattccctc aaaccgggga
     3181 gaaaacttcc aatgtgctac tgttcattgg ctttagcttg atctttgcga cggctggcta
     3241 ttatttttgg aatcgtcgga actaaggtga taagatgaaa catgtaaata gtagaaaaca
     3301 atacgcgcga ccgcctccta aaaaagtggt cgcgctattt ttgtattcaa aactaacaat
     3361 gaaaagagga aaaaatatgg aattgaaatt tgttgtacca gatatggtcg agacgttcgg
     3421 aaaactaaca tacggtggtg aaggtgaagt tctatcggaa gggtatggtc gaaatactac
     3481 aattgttggt cgaagttacc atctgttttc aaataaacaa cgggccgatg atattgaagt
     3541 ggtggtagct gcggaagccg gcgaaaagga ttttgaccaa gatcaaccac taaaagctgt
     3601 aaatccccac ttaattgcca aaggctatga aattgagaat cgtgggttta ccgattacgt
     3661 gttgtatgtt gatgatttag tgaaagcata ggaggaaaag aagatgagat taacagaagg
     3721 tattgtcgta gattcaggat taacctttgg caagttgcgt ttttccgcat tacgtcgaga
     3781 agtacgcaag caaaatgagg acggaacggt tagtaacgag gtaaaagaac ggacctataa
     3841 tttgaaatcc tccgcgcaag gtcgaatgat ccaagtcagt attccagcaa atgtaccctt
     3901 acgcgagttt gcttatgatg cagaggtaga gctggtcaat ccaatcgtgg atacggtcgc
     3961 caattatgtt tttcgggaag gaaccaccgt taattggttt atcaaggcag atgatttagt
     4021 cttgaaacga caactgaatc aaggaaaacc tacgaaccaa aatgaaggaa agaaataggt
     4081 gaagaatatg gaaattaatg ttgaacatat tttagattgt ctcgatcaat ttggcaaagg
     4141 tgagctaacg gaggaacaac tcactaacgc attaacctat gatgaaaaaa tgtttctgat
     4201 tatgcatcaa gggttactgg aagtaggtaa cgatgccaaa gaagactttg atgtactgga
     4261 ttggttaggg gaagaagagt ctttctttat ggttgtcgag gtaaatgaaa gtttatgtcg
     4321 gcaggcagaa tctgttttgg aagaaatcga tgtggagatg ccggatgcca tcgaaagatt
     4381 tttaaaacaa ttagtggaaa cgaaacaact tcccgtagta gtaaatgact agcataactt
     4441 tttgaaattg cccaatgaaa tcctattggg ctttttcgac tggaggtgga aaccatcaaa
     4501 atgtacaaag gacatcgcat acgcgcagga gatcaacact tgatttatca ctttgttctt
     4561 ggctggctaa tctttttgtt cattggttgg atgagcgtct tttatttaca agaacttaga
     4621 caatttgata tttctaaggt atcattttcc actagtgaaa ttggctggtc tatgaaggat
     4681 gtaatctgct tgctaggaag tctcgctctt tcaggaagca tgatgttact ttatatacac
     4741 ttttttcagg atcattggcg aagtctttgg catcgacaaa agttagctcg gatgattcta
     4801 gaaaaccatt ggtatgaagt aaagcagact caaggtgaag gttttttcaa agatttgaat
     4861 agtagttgga ccaaagagag tatcagttac ttccccaaaa tttattatcg aatgaaagat
     4921 ggcttacttt ctattcgcgt tcaaatttca ctgggaaaat atcaggatca gctattgaag
     4981 ttggaaaata agttagaaag cggactgtat tgtgagttag tagaaaaaga actcaaagac
     5041 tcctatgtgg aatacacctt gttatacgat atgattgcca atcgaattgg gatagaagaa
     5101 atagtggcag aaaatggggc gttgcgattg atgaaaaatc aagtatgggc gtatgattcc
     5161 ttgccgcata tgttaattgc tggcggcaca ggtggtggga agacctattt cttactcacc
     5221 attattcaag cactattgaa gtcagatgca gaattgttta tcctcgatcc caaaaatgcg
     5281 gacctcgccg atttaggtac gataatgcct cacgtatact cccaaaaaga agaaatttct
     5341 gaatgtgtgg aagattttta cgaacgcatg atagctcgta gtaggtcaat gaaagaaatg
     5401 cccaactaca aaacaggaga gaattatgcg tatctcggac ttccgcccaa ctttttaatc
     5461 tttgatgaat acgtggctta tatgggtgcg aatcgttttc ctacaagcat cgaatagatg
     5521 tgaaatggaa gcctattgag attatcaata gtgacaacta atgttttgag agatggaatg
     5581 aaagccttca agttgagctg aaacatcccc gctaatactc ctacatgctc taatcaatga
     5641 agtagattgg ttttggtatg aagctaggtg aagtcgtctg aaatctgcct aagtgcttga
     5701 gcataaggct gactataggg cgggcggctg aaaaagataa ctatggtaag aatgtctgaa
     5761 aatgactgac gaatctgcga atgtacgggt ctaaaaggct aatcttattg aaagatagga
     5821 acatacggac gtatggtgag ataccgacaa gtaaggttcc atgatatgaa aatcgggata
     5881 gctaacgaag aagctataat ttcacaggct catagtagat acctaaggtt atacatgata
     5941 gctaaaaaca ttggaacgat gtaagcagaa aactatcaat taaatgacta cttctagata
     6001 ggtgcatata agtaataacg aaattgcttt gtttctgtga aagtaagggc acagtaccga
     6061 tgaagcgtct aacaaatgtg gagggatagc cctaagtcta tttaataaca atgaatgttg
     6121 aaacagatga agttcaagga tctggtaaga acgtgggaaa atccgttatg gaggtgccac
     6181 gcttgaacac aaaaatacgt tactgggaat attatgatct acaagaaaca ttcgatcagc
     6241 tatttactca aagtagaaac gggaaaaagt tttatcaatt atatgagtta atcatttctg
     6301 aaaataatat tcttttagca taccgtacta tcaaggcaaa caaggggtct tcgacaccag
     6361 gaacggattc cttcacaatt gataactata aggaaatgaa ccaagcagag tttatccatc
     6421 ttattctcag tcatttagaa aattacaaac caaaatccat aaagcgagta atgataccta
     6481 aacccaatgg tgagaaacgc ccattaggaa ttccatgcat gattgatcga atgatccaac
     6541 agatgttcaa gcagatactg gagccaatat gtgaagctaa gttttatgaa catagctacg
     6601 gtttcagacc attgagaagt gcaaagcatg ctctaggtcg cattatgtat ctcataaaca
     6661 tcagtaaaat gcactatgca gtagacattg atatcaaagg tttctttgat aatgtaaacc
     6721 accgtttact gataaaacaa ttatggaata taggaatttg tgacaaacaa gtcttagcta
     6781 ttctttcaaa atctctgaaa tcacccattc aaggagaagg tatcccgagc aaaggaacga
     6841 tccaaggagg tatcatttct ccactattat ctaatgttgt tctaaacgac ttagaccatt
     6901 gggtctcaaa gcagtggcat acctttgaaa caaagtatcc ctatactaaa ggttacaata
     6961 aatttcgtgc tctaagagat acaaatctaa agcaaggcta tattgtcagg tacgcagatg
     7021 actttaaaat catgactaat gattatccga cagcattaaa atggttccac gctgtcaagc
     7081 tttacctaaa agacagactt aaattagata tttccaacct gaataattca tagctttaat
     7141 tctttggcac attttattga aatatgtatc actaatatcc ctatcaactg attttgacta
     7201 aaaagttact tcaaacattc actgttcaaa aatattgagg aataaatttt gggtgtaagg
     7261 cattttgagc atacttctcc ctggataact tcccagtaaa aaaatcgtta gattgttgga
     7321 ttagatccat tgactggctc gcctcaggcg agcaaagacc ctgtagaatt cattctgata
     7381 cacatgataa ctagataatt tgagatagac tcgtctaccc gtttggacta atttcccagc
     7441 aactttcaga aacttcaatc gaatagaatg aatggtcatt cccttttgga cttcttcaaa
     7501 gccaatcgtt cttaagaagt tgactaagtt gtaagctatc aagctgagca tcattcggac
     7561 atgattctcc aaaaagcgag gactgtctgt tttgtcaaag taaaagccag ctttcgcttc
     7621 tttaatgaag ttctccattg tgccacgttt ggcatagata gaaaagatgg tatcaggaga
     7681 aacattttct gatagattcg tcacgataaa ttcatgtcga aagagtagtt cgcccgcttc
     7741 acgtgttgag cgtatacaca cgcgacgact ttgtgaccag gattgtgctt gataagtggt
     7801 cgagaagtac tgaacttctc gttcttccca cttttgatta tcgccataaa gtactgattt
     7861 ctcagctatt tgacctagtc tacgattatt cttcagtcga ataacataat gactcttttt
     7921 tgattcacac gaatcataca caccaggtgt agcgaacccg ctgtctccac gaaccaagat
     7981 gtcagtgttt ggtagagagt gattatagtg ctctaataaa ggtgtgagaa actcctttac
     8041 gccttttgat gtatattgat ttcctgaacg tagttcagct tttaagaagt caccagtcaa
     8101 tccgtcaaaa gctaccaatg gatggtaacc ataggtttgg tagtgtgtat tataattcgt
     8161 ttgttcttgg tgaccaaatg tatctgaatg ggtcgaatct aaatcaatga ttaattccgt
     8221 atcattacgg atgaggcgtg ctttatcgat aagttcttgg ttcaaagctt gaagttcatg
     8281 gatattctcc tcagatagtc gatctaaaaa tcgggaaagt gaagattgag aagcgagttc
     8341 tttcctatct aaaacagctt taaacacagg atcttgtctc aggagattag ctgcagaatc
     8401 agctgagtaa ccagcaatta attgcataat gaactgctca agtatggata agttgtcatg
     8461 agtaaaatac gctcgttcgt cttcaatgtg aatgtgttgc ttagccagtt cagaaaaacc
     8521 aaagatgttc atcagctctt tcactaggac gagacccgaa tcactcgata attgaccacc
     8581 tgtatgagaa atagtgatat ttgaattgaa ttttacttgg tttttgtgta agctagtcat
     8641 gaaaagaact cctttctttt ggttggttta gtcactttaa ccatagcaga aaggggttct
     8701 tttttcatca cttaacgggt gaagatgaaa aagtaattgt aagctgccgt gaggcagttt
     8761 cacatggttt taat
//
LOCUS       DAFFSV010000115         8514 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFFSV010000115 DAFFSV010000000
VERSION     DAFFSV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.115"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..431)
                     /locus_tag="LR110_002348"
     CDS             complement(<1..431)
                     /locus_tag="LR110_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823292.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKLCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPL"
     gene            complement(728..3109)
                     /locus_tag="LR110_002349"
     CDS             complement(728..3109)
                     /locus_tag="LR110_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL4823293.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3099..3746)
                     /gene="recU"
                     /locus_tag="LR110_002350"
     CDS             complement(3099..3746)
                     /gene="recU"
                     /locus_tag="LR110_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBL4823294.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3810..4349
                     /locus_tag="LR110_002351"
     CDS             3810..4349
                     /locus_tag="LR110_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBL4823295.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4439..4858
                     /gene="gpsB"
                     /locus_tag="LR110_002352"
     CDS             4439..4858
                     /gene="gpsB"
                     /locus_tag="LR110_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBL4823296.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4880..5247
                     /gene="rnpB"
                     /locus_tag="LR110_002353"
     ncRNA           4880..5247
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LR110_002353"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5456..6622
                     /locus_tag="LR110_002354"
     CDS             5456..6622
                     /locus_tag="LR110_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBL4823297.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6668..8164
                     /locus_tag="LR110_002355"
     CDS             6668..8164
                     /locus_tag="LR110_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBL4823298.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8245..>8514
                     /locus_tag="LR110_002356"
     CDS             8245..>8514
                     /locus_tag="LR110_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL4823299.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISM"
BASE COUNT         2726 a         1512 c         1779 g         2497 t
ORIGIN      
        1 tccaaaggat tatataaata agtgacgtat ctatctaaag gtgtaataaa aatgataagt
       61 gataataaaa aaataccatt tataactaaa cataaactaa tggatttggg cggtttcagg
      121 ctcatcagaa aaatatagat tctggtgaac aacaaccata aaaaagtgag aatcattgca
      181 tgagagcttc tttccataaa ttttccttta agagtagtgc ttaaaagtac agaaaaaata
      241 ataaatgcaa ggataggagc aattaaagta aatttattta ataataaaca taatttttca
      301 gcttttttat tcttggaata acgaggtatt gttttcgata aaaaaagatg gtgcagacaa
      361 attatgataa ttaagcatac tatcagataa ctcaaaatga agccttcttt caaaaaatta
      421 ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt aagaagtggc
      481 actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta aatggataaa
      541 gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat gttagatcac
      601 acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt acatgatttt
      661 tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag acgtggtttc
      721 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
      781 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      841 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      901 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      961 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
     1021 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
     1081 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
     1141 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
     1201 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
     1261 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
     1321 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
     1381 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
     1441 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
     1501 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
     1561 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
     1621 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
     1681 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1741 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1801 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1861 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1921 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1981 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     2041 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     2101 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     2161 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     2221 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     2281 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     2341 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     2401 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     2461 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     2521 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     2581 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     2641 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     2701 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2761 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2821 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2881 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2941 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     3001 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     3061 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     3121 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     3181 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     3241 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     3301 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     3361 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     3421 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     3481 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     3541 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     3601 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     3661 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3721 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3781 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3841 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3901 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3961 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     4021 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     4081 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     4141 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     4201 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     4261 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     4321 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     4381 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     4441 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     4501 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaaac
     4561 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     4621 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     4681 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4741 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4801 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4861 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4921 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4981 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     5041 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     5101 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     5161 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     5221 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     5281 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     5341 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     5401 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     5461 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     5521 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     5581 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     5641 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     5701 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5761 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5821 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5881 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5941 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     6001 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     6061 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     6121 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     6181 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     6241 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     6301 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     6361 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     6421 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     6481 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     6541 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     6601 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     6661 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6721 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6781 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6841 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6901 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6961 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     7021 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     7081 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     7141 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     7201 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     7261 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     7321 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     7381 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     7441 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     7501 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     7561 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     7621 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     7681 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7741 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7801 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7861 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7921 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7981 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     8041 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     8101 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     8161 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     8221 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     8281 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     8341 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     8401 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     8461 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatg
//
LOCUS       DAFFSV010000116         8427 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFFSV010000116 DAFFSV010000000
VERSION     DAFFSV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.116"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..231)
                     /locus_tag="LR110_002357"
     CDS             complement(<1..231)
                     /locus_tag="LR110_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823300.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIAL"
     gene            complement(439..1365)
                     /gene="glsA"
                     /locus_tag="LR110_002358"
     CDS             complement(439..1365)
                     /gene="glsA"
                     /locus_tag="LR110_002358"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBL4823301.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1388..2599)
                     /locus_tag="LR110_002359"
                     /pseudo
     CDS             complement(1388..2599)
                     /locus_tag="LR110_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(2634..4247)
                     /locus_tag="LR110_002360"
     CDS             complement(2634..4247)
                     /locus_tag="LR110_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4823302.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(4359..4568)
                     /locus_tag="LR110_002361"
     CDS             complement(4359..4568)
                     /locus_tag="LR110_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBL4823303.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            complement(5557..6771)
                     /locus_tag="LR110_002362"
     CDS             complement(5557..6771)
                     /locus_tag="LR110_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL4823304.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(6899..7369)
                     /locus_tag="LR110_002363"
     CDS             complement(6899..7369)
                     /locus_tag="LR110_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL4823305.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            complement(7366..7608)
                     /locus_tag="LR110_002364"
     CDS             complement(7366..7608)
                     /locus_tag="LR110_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBL4823306.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(8154..>8427)
                     /locus_tag="LR110_002365"
     CDS             complement(8154..>8427)
                     /locus_tag="LR110_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBL4823307.1"
                     /translation="QQIHFTSENPVLLLERLKQEAGKDIWICGGANLVQQLVSKNMID
                     KYYISVIPTLLGNGVRLLGNIDREIKLRLCKTQTYNGITDLIYMRR"
BASE COUNT         2394 a         1798 c         1347 g         2888 t
ORIGIN      
        1 aagagcaata ccatgtttga atacgagttt atacttatgc ttctctctta cttgttcttt
       61 tatacaatat tctagacact cggctagact ttctagatcg tgaaggatcg ttggtgtagt
      121 cacccttaga gaaatagctg tttgttcgat gtctatttct tggaattcaa ataattgttc
      181 acacagatac acttttctaa atatatttcg ctcgatgtaa ttttccagca ttcctgatac
      241 acgcctccag tttttcttat gattttattt aatcatatct gtaaatcgta tttttgtttc
      301 ctttttattt agaaaagccc tttcaaaaac caataaaaaa agaaactggg acaaaagtca
      361 aacaagctca caatttcagc ctcattgtag gtagctttga gccgtttgga cttttacccc
      421 agcctctttc aagaatggtt attcaaagat gtcagcgtct agttctttga ctacatcttt
      481 caacaattgg ataccagctg cgctgttacc aaatggatct agtgccgggc tgaagacacc
      541 tatgccgtaa cgatttggca cagctgccat caacccgccg cctacaccgc tttttgcagg
      601 tacaccaaca tggacggaaa actctcctga ttcatcatac aaaccagcag ttgtcataat
      661 tgatttgacg attgtagcac tttcttcagt aatgatttgt ttgccattcc aaggagcaat
      721 tcctttattt gccagcacag cagctaattt tgctatgcct gtagcggtga cattgaggga
      781 gcattgtctg aaataagcat cgagtatctc cggtacgtct ccttcgatca tttgattacc
      841 tttcatatag taagcaagag agcgattgat atcacctgtt cttgattcag aatgatagat
      901 ttcttcattc aatgtgatat taggatcatc acagatctct ttcataaatg acaagattcg
      961 ctcaaatttt tcatctgcat ctttaccggc aatcaacgag gttgttgcaa tcgctcctgc
     1021 attaacaaaa ggattcattg gatgtttgcg atgattgatt tccatattca tgatagaatt
     1081 gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag actttttcta ttccgttgtc
     1141 tttgattgct aataacaata cgggaacttt tgacatgctt tcgatggcaa agcgcacatc
     1201 ggcgtctcct ccctcaatca gttgttttgt atccaaatca taaatcgcca tacctaattg
     1261 tttgggatcc acttcagcta atgctggaat gtaatccgcc acttgtcctg tttgataata
     1321 gtggaaattt tttccaatca attgttctaa ttcttttact tccatttgaa taaccagctt
     1381 tcatttttta tttttctgca tgctggaatt tatgacggca tgcgtagatc gtaaatggaa
     1441 taactaagac gataagataa gaaacaatca gaatcgtttc ataaatccca tgctgtccag
     1501 ctgcgatact agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa gtcaaaagac
     1561 ctaaacaagc aacgattact tttacaactt ttccgcctgg aacctgatag gctctttcaa
     1621 gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa aaacataaag tacaacatca
     1681 ggtagattac aacagtcaat gacatcgctg caagaaatga aagattattc cccccgccgc
     1741 ttaatgtaaa gacggctgcc cagattgttg caacgatccc atttgcgatc atcaaatgag
     1801 caggaacacc gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct tcttctgctg
     1861 ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc aatttctccg atgacaccta
     1921 aacaaacaag aatcgcaaga attttcgcaa tccatgcaag atttggatca atgtgtctta
     1981 ataggaattc taacgattga atcacccctg tattaagaga caaaccatgt tgaggtactg
     2041 tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata accaatacaa
     2101 acattgctaa tggataatcc tttttagggt tttccatttc atttgcatga ctggcagatg
     2161 tttctacgcc catatatgct aaaataaagg aaacaaaaac aaccagcgta ggcaatttcg
     2221 aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg cctgaagcga
     2281 cataatggat ccctaagcca aacaagacta atgctggcaa caagatacca gctacaaaac
     2341 cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt aaaatagccc
     2401 agaaaataat caataggatt gccaatttca gatatttatt gttttgtata gcaccaatac
     2461 ccgttgcata ggataatgca ccagttaaga aatacagcat ggtcaagtag ccaactgtta
     2521 tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat gtgcgactga
     2581 cccaagtata gacaccgccg gtttccctta gttaaaaata ttactcttgt tgattactca
     2641 actcttccaa taaatcggta atatatctca atttcctgta ttctttcgtt atcctcattc
     2701 gttgttgctt catgtatgag gattttttct atcatggcat ttaataatgg tgctgtcagt
     2761 tcctttggta cggaatattc cttgattaac tcaatccact tttcagcacc tatcatatcc
     2821 tgttccattt tacttagttc ttctcttagg cttgttatct gctgttccag ttctatctgt
     2881 tctttttggt acttgctgga caacatcaca aaatttcgct ctgttatctt ctcacaggct
     2941 ctatcttcat acattttcgc aaataaacgg tcaatctcat tttgtctgtt ctcggctttc
     3001 ttcaatgtac ttgccttttt cttcttttcc cgtattctct ctgcattgcc agctttctgt
     3061 atcttgttca ataccttttc ttcgtcctgc tgtactgcct tagcccaata ctgcaagcgt
     3121 tccaatacgg cttgatacag cacatcataa cgaatgtagt gcatagaaca attacctttg
     3181 ccaaactgcc cgtagtaact gcaagcataa taactgtaag gcgttttatt tgtcttattc
     3241 gttccaaatc tcatagacca accacaatcc gcacacttga caagccctgc aaaaatcggc
     3301 gttgcctttt cctttgtctg tctgcgtctt gattttatct gctcctgcac acgatagaac
     3361 acttccttgt caataatcgg ctcgtgagtg ttttctactc gaaaccattc actttcgggc
     3421 ttacgcactt tctttttgct cttgaacgat accgtagact gcatattgtg aacactgttt
     3481 ccgatataaa cctcactttt caatatcgcc ttgacatgag caatcgtcca ctcataacgc
     3541 ttactttcgg gttttccctc aaagatatgt gcaaaagtac cgtatcttgt aaaatttagc
     3601 catgacgctg tcggaacttt ttctgctctt aactgctttg tgatttttgc acttccgtaa
     3661 ccttgatagg ctagggaaaa gattttttca ataatccatt ttgtttcatc atcaaccaac
     3721 agttttcctt tgatgtcagg gtgtttctta tatcctaacg gagcataagc accgtaatga
     3781 gccccctccg caaactttgt cttaaatgct gatttgactt tacggcttgt atctcttgcc
     3841 acccattcat tgatgatgtt cttaaatggt gcaatctcac tttcgccttt ttcgctgtct
     3901 acaccgtcat caatcgctat gtagcggaca ttatgactag ggaaatacaa ttctgtatat
     3961 tgtcctgtca taatataatt tctgccaaga cgggaaaggt ctttcgttac aacacagttg
     4021 atttttcctg cctctatatc ctcaatcatt ctttgaaaac tcggtctgtc aaaatttgtt
     4081 cccgaccagc cgtcatcaat atattcatca atgacattca aatgatgttc ttttgcatat
     4141 aaacgcaaca tacttctctg tgtggtaatg ctggaacttt cgccctgtaa ttcatcatct
     4201 cggcttaacc taagataaag tgcagtattg taaatctgtt gtttcattgt cagttatcct
     4261 cctttataac taacccgtgt ttacaatacc tgcttgcaag taaagtataa cacgggtact
     4321 ttttgtcatt caatctattt cttacaactg caccgatttt atgcgattgt ctgctttgct
     4381 ttttccagca tgacatcagt tagaagttct tccattgttt tgcccttttc ggaaaaatgc
     4441 tcttttacta caatctttgt ctttcctctg ttgccaatgc cacacttgcc atttttcttt
     4501 tgaattgttt ttacagcatt atcaataaca cataccccct ttctgtaaaa tctttctaag
     4561 caattcaacg gcttttgtca tagcccacag gagttccacc tctgcatagt tcttatgtag
     4621 ccgtacccat tgcgtgcgac gctcttaatg ctcaagctgt ggctgtacgg gagtatcatt
     4681 atctgacagt acaggtcatg gcgataaaag aggacaaatc ttttacaaga acacaaatag
     4741 gttttcgctc gctcttgcag gggaaaggtc gtggcgtatt tgtttcaacg gctagctgtc
     4801 tgtcaaacgt attgaatagc tttattcagt tgtcaaagaa cgattgaaag aaaaaaatcg
     4861 tctttcctat atatcgcgtt ggaaaagagg agaaacgtaa ccaaaatcca aaactttttc
     4921 cattattagt acaaatggaa cggttcattt tggcaatcaa cggttgattt tctccttttt
     4981 cactactatc tgtacagcaa acgtccattt gctaagttta aatggaaaaa ttttaacttc
     5041 ttttcatgca ccatataggg atagaaaatg tcattttgct aagttgatga tgaaaaaaga
     5101 atattttcct tttcacaccc taactaccca aaccataccg ttactgccaa caatagaatg
     5161 aaatattttc tttgcaccat atagggaacg gaaaccttaa attgttaagt tgagaacaaa
     5221 gtattttcat cttttcacat caacactcaa ctgctaatgc ctgtttgaat acaaaatgat
     5281 gatgtttttc ctcttttcac agaagactgt ccactaatca cgaatttccg tcattttttc
     5341 aaaacttttt tcttctttca cttcatatag ggacagcaag ccccgattta atgacctatt
     5401 ttcaaaaaaa gtttagattt ccttttctcg caccatatct gtacgcaagt agccattttg
     5461 ttgcagattt aataaaaaat ttatttttct tctttcactt cacataggga atgaaaacag
     5521 ctctttgctg actaaatctg aaatttttca attacattaa aaaataccgt cattctcttg
     5581 tggaacagcg gtatctatac gttcttctat ggttgatttc tctaattgta ataaatgttt
     5641 atgcttgtct tttagttctg cctgctcaat catatctttc agtatcgttg tacgattttc
     5701 tctgtctaat gcctgtacca tttttatggt tggtgcgact tgtctttgta gccaatgcaa
     5761 cgctttttgt aaagtgtaag gctctggctt tgtggttaag cgtatcggct ctctgttttc
     5821 ccctacaaac catgcccaat catcatttgt tatccattga ctttttggct tgtcgtcctc
     5881 tctgtcaaca aagcggacat aatgattgat gatagaaaaa gcggtgcgtt cagcgtcccg
     5941 ataggtcagt aaatctacaa ccgcataata ggctcgttcg ttcttcatgc gtatttcaaa
     6001 acggttctta atctccgtat cttcaatttc tgtgccattc ttgacatact gctcataatt
     6061 tttctgataa gcacacatat ataattcgct acttgttgaa ccgagatata aggtttctcc
     6121 tgtattcttt ggtaaatcgc taccgcattt ttctgtaccg ctgtaatctt tctgcatacg
     6181 gaagtaggac atacattcgc cagccttgta tttttccttt aactttggaa tatccaatat
     6241 tcctgcttta tcattgatag ccaaatcaag ccgtttcatc acaccaccag ccgtcataca
     6301 atccagcatg aagtcatacc atgagcgttc ctgtgccagc agataacatt ccatttgccg
     6361 acagcctttg cctttcagtt ccaataaaac acctaaatgc tcctgcatgg aacacatgat
     6421 attgatgtcg ccaagtacat atttgctttc atatccgtat tttccataat cttcataaag
     6481 catgtaatta gatttcagtt gtaatacatc acggataatc gccaatgcgt ccgtagtagg
     6541 aaagcggaca cgaaagtaat caatcaataa gaatagtggt tcttgcggat tgaaacgctc
     6601 aatctgtttt tctatgattt ctcttaattc gtcattcaat ggctgttttc ctttttcaat
     6661 gcggttgtaa tattcacggc tgataccaca ggcaacagca agccttgttt gtgttactcc
     6721 gtattcctca cgcttttgtc ttagttcttc aataaagttt ttatcattca ttcttcaatt
     6781 cctccaatca aaaaaggcaa ctaccataat tgataattgc ctgtcattaa aaatctgtat
     6841 agaaaaagca gtcaatccta agactaactg ctttgtgtgt atggatataa aattgcttct
     6901 aatcttgaat aggtatccaa atttcaataa ctgaattatc actattccaa tgaaaacgat
     6961 aatcgtattt ttcaaagtgt aggataatat cttttatagg aatatacgat gtactaggaa
     7021 taatttcttc ataaatcttg ccataagttt catatatttt atccatagca ccaatatgct
     7081 ccacaaccaa atatttataa gacggtattt ctttatacag aaagccctct ggggtaatgg
     7141 ttctttgggg aatagaacag aaataataaa gttttccatt tcttctttcc ataaaagcat
     7201 attttaccca atttcctgat tgtgaaagat acgctttttt caaactactg ttaaactttc
     7261 tccaaaactg tgtactaatc tgaatattct ttttttgata attagtcagt tctacttcct
     7321 gcccaataac tgcaaatgcg tttaattcct ttatagaaaa atttatcatt ttaccttgtg
     7381 agcagtgata acggtataac tgtacgcatt taccgttata atacaattat caatcgttat
     7441 ataccagttt tttccttttc ttgtgatttc cgcattagaa aggctaattt tttctttgca
     7501 ccattcaatt acattttcta cattcaaaga taagttcttt ttaattctaa taacgccaag
     7561 ttcagttgtg tgaagtcttc ctaagttttc cagtaattca ttttccatat tttgatacct
     7621 catttttcag caaatttatt tgtatgtttt ataccccaat tataacattc tcatatcagc
     7681 actacaataa aaataattaa gcaccaataa tcttattgaa catctccaac cgtaaatctt
     7741 tcacaccatc agtgataaac actgagctga cagcaattac gacaactacc ataattgcct
     7801 gcacttaaat ttctgcaaaa gaaaacgaca atcaatttct tattttttga aatcaactgt
     7861 ctttctcata tttactattc aattttcata gtgatatagg tacaatttat atcactactg
     7921 taaaaatggg gttttgcctt ataaatacag cgttttccgc ttgttagtgt atagtgtttc
     7981 ttgttttttg tgcccccctt gttagataac gggggctttg aacggctcgc aagctcgccg
     8041 aaccgccgaa gcgaaccgcc cccttgcaag catggcggaa aaatcttaag gattttcccg
     8101 ccaacgtggt cggatcgctc cgtcggttta acagcttttc tgtttctctc tagttatcta
     8161 cgcatataaa tcaaatcggt tattccgtta taagtctgtg ttttacaaag ccttaacttt
     8221 atttccctgt ctatgttgcc caaaagacga accccatttc ctaacagagt aggaataaca
     8281 gaaatgtaat atttatcaat catatttttg cttaccaact gctgaacaag atttgctccg
     8341 ccacaaatcc aaatatcctt tcctgcttct tgttttagcc gttccaataa taaaacagga
     8401 ttttcacttg taaaatgaat ttgttgg
//
LOCUS       DAFFSV010000117         8398 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFFSV010000117 DAFFSV010000000
VERSION     DAFFSV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.117"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(89..766)
                     /gene="nth"
                     /locus_tag="LR110_002366"
     CDS             complement(89..766)
                     /gene="nth"
                     /locus_tag="LR110_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBL4823308.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(773..1471)
                     /locus_tag="LR110_002367"
     CDS             complement(773..1471)
                     /locus_tag="LR110_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL4823309.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1739..2923
                     /locus_tag="LR110_002368"
     CDS             1739..2923
                     /locus_tag="LR110_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBL4823310.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2929..3372)
                     /locus_tag="LR110_002369"
     CDS             complement(2929..3372)
                     /locus_tag="LR110_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4823311.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3598..3885)
                     /locus_tag="LR110_002370"
     CDS             complement(3598..3885)
                     /locus_tag="LR110_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823312.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4056..5408)
                     /locus_tag="LR110_002371"
     CDS             complement(4056..5408)
                     /locus_tag="LR110_002371"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBL4823313.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5681..6127)
                     /locus_tag="LR110_002372"
     CDS             complement(5681..6127)
                     /locus_tag="LR110_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL4823314.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6363..7289)
                     /gene="cysK"
                     /locus_tag="LR110_002373"
     CDS             complement(6363..7289)
                     /gene="cysK"
                     /locus_tag="LR110_002373"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBL4823315.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7429..8316)
                     /locus_tag="LR110_002374"
     CDS             complement(7429..8316)
                     /locus_tag="LR110_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL4823316.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2278 a         1731 c         1401 g         2988 t
ORIGIN      
        1 tttttcatgc aaaaaagata aacgaaacag cttttctcca taataacaag ataattcata
       61 catttatttg caattaaaga aaccacgtct acaggcgtgg tttctttttt agcgcctttt
      121 cttttagacg catccgattt ttcccatctt gacatattga tagcaaagga catacctcgc
      181 acttgggatt tcttgccgtg caatgatatc taccaaaaaa aatcagtgta tgatgtgtct
      241 ttacccataa ttcttgtggt actttccgca tcagtgtttc ttctacttcc atgaccgtag
      301 catctagttt gcagatcctc aggcgttttg agacacgttc cacgtgggta tctactgcga
      361 ttgctggtat cccgaatgca tcccccaata cgacatttgc tgtctttctg cctacaccag
      421 gaagagacat caattcttct cttgacgttg gcacttgccc atcgaaacgt tcaatcagct
      481 gctgtgcaca cgctttgatg ttttttgctt tatttcgata caaaccaatc gtttttattt
      541 taagaatgat ttcatcgatt gaagcttctg ctaacgcatc gggtgtcgga aaagaggcaa
      601 acaaatctgg cgttgcttta ttcactgata catctgtcgc ctgggcactt aagatcactg
      661 caatcaataa ctcaaatgga ttattgtgct tgagttctcc atgtgcttcg ggaaacattc
      721 catacatcgt ttctagagct tccattgttt tttgtttgct taacatattc cgctactcct
      781 ccaaccaatt tttcagtgag actttcggca attcttcttg ttgagctgac tgttctactt
      841 ctctttgaag aagctgctgt tttcttcgtt tttggtcttc ttccacttgt tgttttgtac
      901 gtagattttt tctttcccac gatagcaaaa tgcggtcaac gtaattaaaa ctataagcct
      961 gatttaatac ggcttcccgc agtgctagct tcaaaatttc cggctgataa tggtcttctt
     1021 ctagccattg tccgatccgc tggaactcga tcgaagaaag cgggcgtcca aattcttgtt
     1081 caaataattg atacactgac tggatttctt tttcttgtgt tttttcttcg tatttttttt
     1141 cttgtgtttt taaatactca cctaatagat catagatggg gtaaagatta tatcgatctg
     1201 cttttttacc gttgacaatc gtcgtttcta tcttgataaa tccatttgtt acgagacgat
     1261 ccagtatttg aaaaatctga tcttgtttga ttcccatatc catagcaatc atctgcaaat
     1321 cagggaaagt atctccttca agctgtgcca tatgcaactg tagaacaaat aaaaattctt
     1381 cagattgtag accgatccta tgataatttt taagtaaaag attagatatg actgtttgtc
     1441 cagcgcttaa gtaattttct aaattcaaca tgatttctcc tccgctcaat cacagtatac
     1501 gtaaaaaaaa catgggaagc aagaacaggt ttcttttcta tttacttcac tgatcggtca
     1561 gatttacaat gtcgtctact tgatttttgt ttgttcatgt ttttgatgcg tatcttcact
     1621 tttcggaata aataaattaa gtagatttcg ctgttcctta gaatgttgac aaggaaacaa
     1681 aattactata cactattttt gtttccaagt taacattatt gcataaagga gtttttacat
     1741 gtacagtttc acaaataaac attctaaagt ttttggatac gtctctgtct tattcactgg
     1801 attaggattt actatcgtca gtcctgtact tccgttttta acgctgcctt attctcactc
     1861 catacatcag caagctttct atatcacttt gctcatgtct gtttatgctc tagctgcctt
     1921 cttatctgcc cccattctcg gtagcttaag tgatcatttt gggcgtcatc cgatccttat
     1981 tatcagttta ttaggatcaa gtatcggata tttgcttttt ggtttaggaa attcgatatg
     2041 gatgttgttc cttggtcgta tcattgaagg attgacggca ggagaaatct ccaccttata
     2101 tgcttacttt gccgacatca cggaacctaa tgaaagaacc aaagtttttg gatggatggg
     2161 tgctttagtc ggtataggaa caacgctggg tcctattata gggggtctat tagctgagtt
     2221 ggggaattct gttcctatat ttattggagc actctttact ttcttaaatg ccgtttatgg
     2281 ctacacattt atgctagaaa gccttccaat aaaaaaacgt tcagtagatt taagtttctc
     2341 ccatgtaaga ccttttcacc aattacaaca attattcaag atttcatccg ttattccttt
     2401 attgacagca ggttttgctg tttggttggc agctggctct cttcaatcta tcttttctca
     2461 attttcgatt gacgcctttc agtggaaggc tgggttggtt ggtttaagct tctctttgat
     2521 tggaatcttg gatactgtct cgcaattgtt cattatgcct cgattgctca aaaaattttc
     2581 agaacagcag atcacaagaa tagggatgtt cagtgaaatc ctcgcctatc tattcatcac
     2641 attatctgga attcttctac tgccgatttt gtttttattt gggattatct gttatggttt
     2701 cggtgattct atttttactc ctgtattcaa tggccaacta tccaattctg tctcagataa
     2761 ccagcaaggt cttgtaatgg gcggtaccca aagtatacaa tctctatcgc gcgtcatcgg
     2821 cccgctaatc gcaggacagc tttatgctgc agcaccattt cttccgacgg tattcggatt
     2881 tttgcttatg atttttgcct attatcacta caataaaaaa tagtagcttc atttgctact
     2941 tcgtataaat tctctgtatc gaccgataaa acgaaatact gtgtcaaatt cttcttttga
     3001 catattttca aatacagtag catctcgctg tacccatttt tgatggagtt gctcgtgtct
     3061 attccttatt ttttgtccga ttggcgtcaa ttgaaaatag atttcttttt tattctcttc
     3121 gctttgataa gtgtttatga tccctctagc tatcagtttt tttgttagct tactgatggc
     3181 tcctctagtc atgtaactag cattcgctaa ttttgtcacg ttaggatcag ggattttccc
     3241 gatatgttca atcacttcta gttcatttag agtaaatcct gcaaattctt tttccatttc
     3301 tggtttatta cgatgaataa tctcattagc taaatccaga tattcttcta ttagcagttg
     3361 tcttttatcc atttctacac cttctttttc tttcattata ttgttttttt gtttcctatt
     3421 caacaaagat aaaaatattt tgtaggaaaa cccaatctga aaccaagaaa aaaaggggtt
     3481 gacaaagctt gtcataaccc ctttttctga ttaaaagata gtaaagagac agtcatcggt
     3541 aattgtgatg attttttgtc atcacttaag gatgagcggc cttttcaaaa actgtcttta
     3601 aagatcatca tcagtttcta gcacatatgg ctgaccttca tatgtgtact ctgtattcaa
     3661 tgtgccgtca ttttcatctg agtagatgcg tctactgaaa ggaaaacgat gagaaacatt
     3721 atctttggcg tcatttgctg gcatcgtgta atattcctct tgcgtttcat tcaactgaga
     3781 gagtacttga tctgccaact catcaatatt gatcgttaat ttcttcgaag cacttttata
     3841 atcttctaaa aatgcatctt gttggacaaa atattcttta ttcatcttct ttctcacctt
     3901 tcttttcaaa atcaaataaa gctgcttgtt cttttatgta acagctttat ctttgctgct
     3961 tattgtgaca aaaaattcga agaaagacaa ctaattgtac ttttcacaaa aaaagacaga
     4021 gggtcgtgac aaaattgtgt cacaaccctt tttctttatt gtgcagtttc gatttcgcct
     4081 agcgctgctt gtgcaagtag gttcaagtaa ttccatgggc ggtcaaaatg tggttggaag
     4141 aagaaatctg aaagtgcgag atcttctaca gtcattttat tttgaacagc taaagacatg
     4201 gtatttgctg attgtgtaat gtcgtatttg gacatgaact gagcacctac gatacgattc
     4261 gttccttttt catagactag ctccatcatt actttttcag tagttggcat gaactctgga
     4321 cgataattat cttcaaatac agtcgcttgg acttctagat cgttcaaagt tgcgctttct
     4381 ttggtcacac ctgtcgaacc gatcgtccag ccaaataggt aaagtcctga agttccttga
     4441 gtaccacgat acgccatttt atgatctgtc aagttatttc ccacaagcaa accttgacga
     4501 acggcatttg ttgccaaagg aatatagttt tgcgtgccac ttggattgta gttgactact
     4561 gcagagtctc cagctgcaaa aatgtctgga ttacttgtct gcatgtattc attcactttg
     4621 attgccccgt ttggcagtgt atctactttt ccttcgatca gttttgtatt tggacggaag
     4681 cccacacata aaatgaccat atctgtatca aatgtatctg ttggtgtagc tacttgtttc
     4741 acttttcctt cttcatctgt gataaattca gtaacatttt cgcctagcgc aagagtaaca
     4801 ccgcggtctg tcaattcttt ttctaagata tctgtgaatg gtttatctag atatttattc
     4861 aagatgcgat ctaatccatc aatcaatgtc acttgttttc cagattcgac aaaagcttct
     4921 actaattcaa taccgatata tccaccacca acaattacta cacgttttgc ttctttcgct
     4981 tgtgcaataa tttcatttgc ttgattgtag tttttgcata ggagtacatt tttcgcatcg
     5041 attccactaa tcggtgggat aataggccat gaaccagttg tcatcactaa tttatcataa
     5101 gaaaccgttt ctgtttctcc tgttttcaga tcttttgccg ttactgtttt attttctgta
     5161 tcgacgtctg ttacatcgtg ttccatttta actgttgctc ctaatgaaga taattcttca
     5221 ggatttgagt agaacaagcc tgctgggtcc tttactactc cgccgacata aagtgcgata
     5281 ccgcaagata agaaagaaat attgtcattt ctttcatata ctgtcacttc tgcttctgga
     5341 tgatttttta aaatactttt tacagctgca gtacctgcat gagtacagcc aattactact
     5401 actttcattt atggtccctc cgattaacat tttgtccttc gacaaaacta ctataacaga
     5461 aataaaacgg atacaacagt ttaatgcttg tgaattcaca aaggtttgtt tgtagtttca
     5521 ttccttttca agcataagaa cccaatatct acaagattct tattttaata atattcactt
     5581 ttatatctaa gattgtgaat aaaagacaaa aaaataaaaa agctagcgtt ttttttacac
     5641 agctagcttt ttttaggtat aacacttgta gaaactgttt ttacatttgc gccttttttg
     5701 ctgactcttt acaatccgga cagagtccat acaattccaa ccgatgttca ttaatctcaa
     5761 aatcagtcag tctgcttgca gccatttcta tatcttcaag ccctgggtaa tgaaaatcaa
     5821 caatcttacc gcagttttca caaattacat gataatgacg ctttgaactg aaatcaaatc
     5881 ggctagaagc atcgccgtac ttcatttctt gaacaaatcc aatcgaagta aataagcgaa
     5941 ggttattgta aacagtcgca acactcatat ttggaaaccg atgttcaagt gctttataaa
     6001 tctcatcagc cgttggatgg gaatgatgtt caatcaaata ttctaaaacc gcgtaccgtt
     6061 gaggtgtgat tcgaatgttc tcttttttta acttttcgat aacctcttcg actaacttat
     6121 tttccatgta aacaccccac gttgttattc tcgtcttatc atactatttt tacattttaa
     6181 aatccactta acatgaaaag aaaattcaat tttattgtat tttaatgaaa aaagacgtaa
     6241 aacagtcgcg ttcactattc tacgtctcta ttcaaataat ttttctcatc ataaattttt
     6301 gtgtgttctt ttcatcttta aaagtttttt acataaacct atctggagaa aaaccaagtg
     6361 ccttatatct cttggtaaag ggctgtagat aaataacgtt caccattgtc gggaaccaca
     6421 gctaatacct tcttgccttt tcctaattct tttgcgactt ttagcgcggc agcaattgca
     6481 gcgccagatg agatgcctac taaaatccct tcttttcgcc caacttctct agctgtttcc
     6541 atcgcttcgt ctccactaat agaaaggact ttatcataca cagaagtatc cagtgtttta
     6601 ggaacaaaac ctgtaccgat tccttggatt ttgtgaggtc ccgcttcttt cccttctaaa
     6661 atagcagatt ctgctggttc taccccgatt aattcgattt tcggatacac acgttttaat
     6721 tctcggccag cgccagtgat cgttcctcct gtaccgatac ctgcaacaaa cgcgtccaag
     6781 ccatttactc caaatgcttg atgtatctca ggaccagtcg tcttctcatg cacaagagga
     6841 tttgcttcat tttcaaattg taatggtaaa aagtagccgt tttcttttgc caaacgttct
     6901 gcttctctga ttgatccaga aataccatcc gcaccaggag tcaaaatcaa ctctgcgccg
     6961 taccctttca tcaataaacg tctttcgata ctcatcgtct caggcatcac gatgattact
     7021 ttatatcctt ttgcaactcc taccatcgac aatccgattc ctgtatttcc agaagtaggt
     7081 tcaatgatag tatcacctgg tttcagtaga ccatcgtgtt ctgctttttc tatcatactt
     7141 aaagcaatac gatcttttac acttcctcct ggattaaaaa actcaagctt tacaaaaaca
     7201 tctgctgaat cttctggtac gattttattt aatttgacga ttggcgtttt gccaatcaat
     7261 tcggtgactg agttatagat agctgtcatc ttcaattcct ccttgtcttt gctcttctta
     7321 cttatcataa tacaatctgt tcatttatgg cgagtgattc gtctttgact agcaaaaaaa
     7381 agacaaagat ataccatata tggtaatttc tttgcctttt tatctaaatc aaacgtttag
     7441 ttcttgacca actaataaag aataactgga catagacatg ccatttttcg ccattagatc
     7501 atcaacagac atgccaaatt tttgtgcaat atcccatagc gtatcacctg acacgactgt
     7561 atatgtctga ccattatttg tatttacatc tgttgttgaa gatgatgacg ttgaagtcgt
     7621 cgtagtagtc gttgttgttg tacttgtggt tgttgtagcc gaacctgttg atggcgtatc
     7681 aaaacgggtt aaattataag tattaatcaa tgagttcaat ttggccccat aatttggatc
     7741 ggtagcataa cgacctgtca agtacgctgt tgcatcataa aaacttgttg tattgctttt
     7801 ccacacgcca gcataatgat aatcgcccgt ggaaaaactg gttgaacgaa gaacattcgc
     7861 atggtcttgg aaggattccc aataagaaga atattttcgg aatggctcat tcatcgttac
     7921 ccattgtccg tttacatatt ctttggttgc catataaacg acctgtccgt tatagcttcc
     7981 tttcacaccg aatagattat aatttggcgc actagctaat ccggaagtgc catatgcact
     8041 ttccaacaac gcttgagcga tcatgactga agcatacaag tcattacttg cagcaacttc
     8101 tgacgcagac cagccgattt gatcgataaa cgcttgagga tcagcgactg tttgagatcc
     8161 ttgttcatct gcatgaacat gttgcggtac cgctgtaacc atcatcggcc caactaaaag
     8221 actcgttcca attaaaggag caatcgcttt tttattgatc accatttttc ttttaggtct
     8281 atggtgccgg gcactgcggg tcttcagctc ttgcattcaa attcctcctc aaaataaaaa
     8341 aacttaataa aaagttctct tcttaaaaca aacttaatta attaaattat acttttaa
//
LOCUS       DAFFSV010000118         8273 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFFSV010000118 DAFFSV010000000
VERSION     DAFFSV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.118"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            476..1339
                     /locus_tag="LR110_002375"
     CDS             476..1339
                     /locus_tag="LR110_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL4823317.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="LR110_002376"
     CDS             complement(1468..3090)
                     /locus_tag="LR110_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4823318.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="LR110_002377"
     CDS             3339..3860
                     /locus_tag="LR110_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL4823319.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LR110_002378"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LR110_002378"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBL4823320.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="LR110_002379"
     CDS             5941..6759
                     /locus_tag="LR110_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBL4823321.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="LR110_002380"
     CDS             complement(6862..7059)
                     /locus_tag="LR110_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823322.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="LR110_002381"
     CDS             7228..7806
                     /locus_tag="LR110_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL4823323.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="LR110_002382"
     CDS             7901..8038
                     /locus_tag="LR110_002382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823324.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2489 a         1485 c         1632 g         2667 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ata
//
LOCUS       DAFFSV010000119         8069 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFFSV010000119 DAFFSV010000000
VERSION     DAFFSV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.119"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(133..306)
                     /locus_tag="LR110_002383"
     CDS             complement(133..306)
                     /locus_tag="LR110_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4823325.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="LR110_002384"
                     /pseudo
     CDS             754..3116
                     /locus_tag="LR110_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="LR110_002385"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="LR110_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBL4823326.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="LR110_002386"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="LR110_002386"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBL4823327.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="LR110_002387"
     CDS             5704..6489
                     /locus_tag="LR110_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL4823328.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="LR110_002388"
     CDS             6502..7353
                     /locus_tag="LR110_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBL4823329.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="LR110_002389"
     CDS             complement(7477..7929)
                     /locus_tag="LR110_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823330.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         2714 a         1373 c         1710 g         2272 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattc
//
LOCUS       DAFFSV010000120         8038 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFFSV010000120 DAFFSV010000000
VERSION     DAFFSV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.120"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            22..1176
                     /locus_tag="LR110_002390"
     CDS             22..1176
                     /locus_tag="LR110_002390"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBL4823331.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1198..1950)
                     /locus_tag="LR110_002391"
     CDS             complement(1198..1950)
                     /locus_tag="LR110_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL4823332.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2314..4980)
                     /locus_tag="LR110_002392"
     CDS             complement(2314..4980)
                     /locus_tag="LR110_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL4823333.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLLINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5321..6772
                     /gene="cls"
                     /locus_tag="LR110_002393"
     CDS             5321..6772
                     /gene="cls"
                     /locus_tag="LR110_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL4823334.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7101..7916)
                     /gene="thiD"
                     /locus_tag="LR110_002394"
     CDS             complement(7101..7916)
                     /gene="thiD"
                     /locus_tag="LR110_002394"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBL4823335.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2328 a         1682 c         1445 g         2583 t
ORIGIN      
        1 cataagtaaa gaggtgttga aatgaaaata gggattgatc gtctttcctt ttttattcct
       61 aatttatatt tagatatgac agagttggca gaaagccgtg gggatgatcc tgcaaaatac
      121 catataggga ttggtcaaga ccaaatggca gtcaatcgtg caaatgaaga catcattaca
      181 ctaggagcaa atgctgccag caaaatcgta acagaaaaag accgtgagct aatcgacatg
      241 gtcatcgtcg gaacagaatc cgggattgat cattcaaaag cgagtgcggt aattatccac
      301 catttactga aaatccaatc ttttgctcgt tcttttgaag taaaagaggc ttgctacggt
      361 ggcaccgcag ctttgcacat ggcgaaagaa tatgtcaaaa atcatccaga acgaaaagta
      421 ctagtcatag caagtgatat tgctcgttac ggcttggcaa gcggtggtga agtgacgcaa
      481 ggtgtcggtg ctgttgcgat gatgatcact caaaacccgc gtattttatc gattgaagac
      541 gacagcgtat ttctgacaga agatatctat gatttctggc gtccagatta tagcgaattt
      601 cctgttgttg atggtccttt atctaattct acgtatatcg aatcattcca aaaagtttgg
      661 aatcgacata aagaattgtc gggtcgagga ctcgaagatt atcaggcgat tgctttccat
      721 attccgtata ctaagatggg aaaaaaggca ttgcaaagcg tattagacca aacagacgaa
      781 gaaaatcagg aacgtcttat ggctcgctat gaagaaagca tccgttacag ccgacgaatc
      841 ggtaatcttt acactggttc attatacctg gggctaactt ctctactgga aaattcgaaa
      901 tcactacagc caggagatcg catcggtcta ttcagctacg gttctggtgc agtaagtgaa
      961 ttttttacag gttatctgga agaaaactat caagaatatc tcttcgcaca atctcatcaa
     1021 gagatgctag attctcgaac acggatcact gttgacgaat atgaaacgat tttcagtgag
     1081 acgcttcctg aacacggaga atgtgcagaa tatacttcag atgtaccttt ttcgattaca
     1141 aagatcgaaa atgatattcg ttactacaaa atataataaa atcataaaaa gaacccttca
     1201 cgagtcgtga aaggttcttt ttttatctgc ggaccaaagg agcagtctat aggtcagctt
     1261 agtcgtacta aggatttctc gtttttcact gaatagtttg cgaatgtacc tggtcacaac
     1321 aggatctttt gcgtacgcgt catagtatag cttcatcaat tcttcagtaa ttgattcctg
     1381 tgtaaaataa gtaaaatctt ttttcttcac tttgaatcct tcatcaatta agtatgtttc
     1441 aaattgatcc attacaactg gcgatgtatt aggatcttct tctatcattc ctagttgctt
     1501 ttcgatagga taaaaaacat catcgattga ttccactact ctgattatca cgcaccattt
     1561 gcttgacttt ttcatcaatt tttctaattg aataagatgt accaatgctg gatttgctgc
     1621 tgtaaaaacc atttcatatg tctcatttga ttggataaat tcttctaacg actgtttgat
     1681 aaaggaaaca tttggcaact catttttttc tgcttctttt tttgcatttt ctagcattcc
     1741 tgaagaaaaa tcgatcaaat catagcgatt aatttctttt gctaaggaaa gaaatctgcc
     1801 agatcctcca gcaacgtctg ctgctgtttc agcaggcagg actttctccg ttttcagata
     1861 attttttaca tcttcagcaa tagttacttg cgattcttgc tggatttcat aatattcttc
     1921 agcaaattca tcccattctt tttcattcat ttgatttcct ctaatttttt gttatatgtt
     1981 ttcacttttc ggaataaatc attctcctct tttatctatt ctatctcttt tgatgaataa
     2041 taaaatgatt ggctttccgt ctatcataaa taaaaaaagg gactgtgacc aaagtcgtgt
     2101 caaagtccct ctttccgaat aaacagtgtc caatcagatg accctcagta ttagctcaaa
     2161 atgctgattt tacaacttct tccttaaacg gtgttctcct gccaactctt cggtcacaag
     2221 tcacaatatt caacaaacat gagaagctgt ttgttgaaat tgcaccttgt gactcagaat
     2281 taaacggcag gctcacaacc tcgttgattc gttttattta tttccttgta ctcgtacaat
     2341 gatcaattta gtgatttcca tcaaaacaat tgtcacaagt gcgattccca tacatgtaaa
     2401 gacttctgtc aatccaaatg ctgatgggat agagaagact tctttcagtc ctgggatcat
     2461 tgttaagcag taaagcgcgc tacatacaac tacagctagt aagacacttc tgttagagaa
     2521 gaaccctgct tggatagctg tttgtgtgtt tgaacgtgct gggaatgttt gcaatgtccg
     2581 acaccaaatc aatgttgaaa atgacatggc tacaccgaat tcaggtgaat gctgcatgcc
     2641 aagccattga gcaaagataa cagcaatccc aatcagcaca ccacgatacg ttacagaaat
     2701 caatgttttg tccgtaaaga ttcctgaatt tggatctctt ggcgggcggt tcatcgtagc
     2761 aggttcagct ttttctgtac ctaatgcaat tgctggcagt gaatcattga ctaaattgat
     2821 gagcaacagt tgtaatgctg taaatggatt ggaccagtca gcaactaagg caaagatgat
     2881 cgcaatgatc gcacctaaat ttcctgcaaa tagataagaa atagctttct tgatattatc
     2941 aaacacatta cgtcctactt ctactgcgct gacaatagaa gcaaagttat catccgtcaa
     3001 taccatcgct gctgcatctt ttgccacatc tgtacccgtt cccatagcaa ttccgatgtc
     3061 tgcttgtttc aatgctggtg cgtcatttac accatcacct gtcatagctg agatatgacc
     3121 tttattttgc caagcacgaa cgatacgaat tttattctca ggtgaaacac gtgcataaac
     3181 agtgactttt tctaaaactt cgtttagttg atcttctgtc aattcatcta gttccgtccc
     3241 ggtcaaagcc aggtttcctt cttcaaaaat cccgatttgt tttgcgatag caacagctgt
     3301 tgttttgtgg tcaccagtaa tcatgattgt tttgattcct gcacttcttg cttctgttac
     3361 cgcctgagtc acttcagcac gtggaggatc gatcatagca agcaaaccaa caaggatcaa
     3421 atcgtcttca tcttcaaatg ataaatcaaa attatcaagt ggacggtaag caaaagctag
     3481 tacacgtaat gcgcgtgttg aaaaagcttc attttgctct tggaattttt tcatcaattc
     3541 atccgtcatc ggaacgactt caccatttag caatactttt ttactgcgtt ggaagacgat
     3601 atctggtccg cctttaacaa acaagtaatt tttgttatca attgtgtgtc cagtagacat
     3661 taattttcga tctgagtcaa aaggaagttc tgcttgtctt gggtagttgt ttcggatttc
     3721 ctgataaggc tggtttaatt tttcactaaa gtcgataaac gctacttccg tagggtcgcc
     3781 caatttagta ccgtcttcac tgatcgatgc atcattcgct aaaacactga tttcgatcaa
     3841 gcgacgttcg tcagctgtcc attttgaagg gtcatttgtg aaatccccag tatcgccatt
     3901 acctaaaaag tagtcaacaa ctgtcatttt gttttgtgtc aatgtaccag tcttatctgt
     3961 acagatgata cttgtcgaac caagtgtttc aactgctgga agcttccgaa taatggcatg
     4021 acgttttgcc attttattcg ttcccaaaga taacacgatt gttacgatag actgtaatgc
     4081 ttctggaata gcagctacgg ctacggctac ggcaaacatc actgcgctga cgatatctgc
     4141 agtcatatct cctgtacctt ctccaagata aatacgaaac agttggatac ctaaaatcaa
     4201 taaagacaat gccaaaatag ccaaacttag ttttttactg aatttatcca gacctcgttg
     4261 taacggagtg acttgctcag ttgttgattc caaaaggcca gcaacttgtc cgatctccgt
     4321 attattccca gttgaagtca ctaagaattt accgcgtccg taagttacga tcgttccact
     4381 gaataccata tttgtacgat ctccgattgg taccgtactt gtgatatccg ttacttcttt
     4441 ttctgcagaa gtcgactctc ctgtcagcat accttcatct acttttaatg atcccgcatc
     4501 tagtaaacgt ccatcagcag gtacataatc gcctgcgtct aacaacacga tatctcctac
     4561 cacaagttcg tttgccggaa tcgttttttc cttaccatca cgaatcactc gagcatcggg
     4621 cgctgaaagg tttttcaacg cttctaaaga accttctgct ttttttgttt ggacaacact
     4681 gattatagaa ttcaacatca atactgcaaa aataactaag aattcgatgt agtcacccat
     4741 gaccatctga atcagtgcga caatcagcaa tacgataacc attgcatctt tgaaagtttc
     4801 taagaacaat ttccaagttg gcttcttcgg cttttctttt agctggttca agccatctct
     4861 ttctaatttt tccttcgctt gcgtggtgct caatccatcg gttgagctct gcgtttgctg
     4921 catcaacgag tcgatcgttt ctttatagac cgcttgttgt gcttgttcct tgttttccaa
     4981 atttcatccc ccattttttg atcttggccc tagtcctgtc tgaaaaaaaa gagacctaag
     5041 acaaatacgc atttgtctta ggtctcacca tttcatacaa taccagaaaa ccatttggtt
     5101 aacttactgt tgatattatc gcaacaaacg ttgccggtta ctcccctaag gagatattca
     5161 actgtactaa tatcctacct tattaaccta caaaaagcaa ctactttagc ccgatttacc
     5221 tacattttca tgaaaggagt gaaaagtata ttcaccttgg taggcttctt tcttctatca
     5281 tgctatacta atgttattgt atgtaaggag gaacattttt gtggtatcta gtattataac
     5341 cgccctttat ctattaaatg cacttattgc tttggtggct attttgatca aaccccgaga
     5401 tgtagcagcc atttgggcat ggctcttagt attgtttgcc cttcctgggg tgggctttgt
     5461 attatattta tttttcggac gtggattaac ggataagaaa aaattctatc tccgacaaag
     5521 tgacttgaaa gaattagaaa actttcagtc ttttagagga gataccattg aacattacga
     5581 tcctgacatg ggcgataaag acaaacagca atttgttgac ttcttctctt cattaaatcg
     5641 tatgccgctg acaagaatga attctgtcac tcttctcaca gacggacaag agaaattgga
     5701 ttcactgctt caagatctaa aaaaagccaa acattcgatc catatcgaat attacgcatt
     5761 tgtgacagat aatatcggcc agcaagtctt acatgtttta gaagaaaaag ccgcagaagg
     5821 cgtggaagtt cgaatattat atgatgcatt tggctctcat ggcacaaaag caaaagattt
     5881 caatcgtcta atcaaaaatg gtggacatgt ccatacattt gttacctcac aaagggcatt
     5941 acttcgtttc cgattgaatt accatgatca ccgaaaaatc gttgtgatcg atggaaagat
     6001 tagttatacc ggtggtttca atattgccaa tcaatatgta aatacaacaa aaaaattcgg
     6061 ctattggcgc gatacgcata tacggatttt cggtgccgct tcttctttgc tccagcttcg
     6121 cttcttaaca gactggaacg tctcggtacc tgaagaaaaa aaggtcggct atcatttgaa
     6181 ttatttcttt aaaaaagcag atcgagatga atctaagctt gctgatacat ccatccagct
     6241 tgtttcaagc ggaccgaata acgaaaggga acaaatcaag ctttcattta tcaaattgat
     6301 tacttctgct aaaaaacgtg tttggataca gacaccttac cttgttcctg atgaaagtgt
     6361 cattgctgct ttaaaaatcg caactgcctc tggtgtagat gtgaaaatca tgattcccaa
     6421 caaaccggat catcctttta tttatcgagc aacacaatat tatgctcggc agctgatcaa
     6481 ggaaaatgta caaatccttg tctatgagaa cggcttcctc catgcaaaaa cattgataat
     6541 ggatgatgaa atctgcatgg taggttcagc aaatcaagat attcgtagct accgattgaa
     6601 ttttgaaaca agtgctgtca tttacgatcc tgagttttta gaagaacttg ctactcagtt
     6661 caaagaagat gagacacatt gttcatccat gacaactgaa acagtcaagg aaatgtctaa
     6721 ctggctatta ttcaagcaac aaatttctcg attattttct ccaatcctat aattttttta
     6781 ttcaagtaga gaaaaatata ctaagattaa agggactgtg acaaaaatct tgtcacagtc
     6841 ccttcttccg aacaaacggt gttctcctgc caactcctcg gtcacaagtc acaatattca
     6901 acaaacatga gaagctgttt tttgaacttg ttccttgtga ctcggagctg gacggcaggc
     6961 tcacgacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata agtccaacca
     7021 agttaaaaac ataagaagct gtttttactt ggctgttctt attacttgat gcagggcaac
     7081 tttttcacaa cttctttctt ttactctaat tcaccagctg cccaatgatt gatggggcca
     7141 tatttgtgcc ctacatttat ttcttcacga attgcacggt ttacatagtc ttttgccaat
     7201 cggatcgctt gttcaactgt ttttcctttc gctaactcag ctgcaataca tgcagatagc
     7261 gaatcgccag ttccgttgat ccggtcggta ggataaaatg gtgcatttaa ccaaaatgat
     7321 tttcctgatt cgaacaatac gtagtcattg actgtttctt gctgcgcatt tttagcatga
     7381 tgtcctttga tcataacgtt ttttgcaccc atatcttgca gttcttttgc tgcttgcaca
     7441 aaatcttctt cagctgctaa tgtccgtcca gtcagctttt ctgcttcata aaaattaggt
     7501 gtcagtactt cggccaaagg gacaagcttt tctttcaagc tttgcaaagc agattcttct
     7561 agtaaaagat ttccatgttt cgttacgatt acgggatcca cgactaatgg tccaaaatca
     7621 tatttttggt aattttttac aactgtctca atcaactttg aatctgctag catacctgtt
     7681 ttagctgcac gtatttgaaa gtccttcgca atcagttcaa attctttgtc gataaaatct
     7741 gttggcaatg tcacacttgc tccgattccg taagaattac ctgcaacaca tgcggtcatg
     7801 acagaaacac cgtaaacatt tcttgtaaag aatgtatgca tatctgcttg catccctgcg
     7861 cttccatcgc tatctgatcc tgcgatcgtc attgcttgta catactgatc ttccatcaaa
     7921 tcgattcctc catttttata aaaagatttt tcataatact gttactgttt ctatcatatg
     7981 tttttcaaag tatccctcag taaatatacc actacctttt cttggtaatc aaatttat
//
LOCUS       DAFFSV010000121         7875 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFFSV010000121 DAFFSV010000000
VERSION     DAFFSV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.121"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            111..812
                     /locus_tag="LR110_002395"
     CDS             111..812
                     /locus_tag="LR110_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL4823336.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1421..2437)
                     /gene="tsaD"
                     /locus_tag="LR110_002396"
     CDS             complement(1421..2437)
                     /gene="tsaD"
                     /locus_tag="LR110_002396"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBL4823337.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2434..2898)
                     /gene="rimI"
                     /locus_tag="LR110_002397"
     CDS             complement(2434..2898)
                     /gene="rimI"
                     /locus_tag="LR110_002397"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL4823338.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2905..3447)
                     /gene="rimI"
                     /locus_tag="LR110_002398"
     CDS             complement(2905..3447)
                     /gene="rimI"
                     /locus_tag="LR110_002398"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL4823339.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3431..4153)
                     /gene="tsaB"
                     /locus_tag="LR110_002399"
     CDS             complement(3431..4153)
                     /gene="tsaB"
                     /locus_tag="LR110_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBL4823340.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4344..5324)
                     /locus_tag="LR110_002400"
     CDS             complement(4344..5324)
                     /locus_tag="LR110_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4823341.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5348..6832)
                     /gene="galT"
                     /locus_tag="LR110_002401"
     CDS             complement(5348..6832)
                     /gene="galT"
                     /locus_tag="LR110_002401"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBL4823342.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6844..7833)
                     /gene="galE"
                     /locus_tag="LR110_002402"
     CDS             complement(6844..7833)
                     /gene="galE"
                     /locus_tag="LR110_002402"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBL4823343.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2203 a         1696 c         1397 g         2579 t
ORIGIN      
        1 atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt tacaaattct
       61 ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta atgcgtgtta
      121 ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa catttctcgg
      181 atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa aaaggacaga
      241 tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa ggactcgttc
      301 gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg aatacaaata
      361 acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca gctcaagcga
      421 tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta gcacgaaaaa
      481 atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa atacatgaat
      541 tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa aatttattca
      601 ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt ccttatccga
      661 tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct ggacaagtaa
      721 tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt actttctgta
      781 agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca gtgaccctga
      841 taaatatcag ggtcactgct tattttcctc cctaaaactt ctgaaagatc cggcagtttt
      901 agatcactgg acaagactca aagattttct atgtgtgttt cagttatgta atattcactg
      961 tttgcaaaaa atactgttag attaactaga tgaagcgtct tgtcctcatc cgatctagat
     1021 tattcgatat tcctcgacaa taaacgatta ctcagtgagg gcaatcagag gaacggataa
     1081 tgataaataa gctgtaaaaa atagcaaaat aacaataatg attaaaagta tgtaaaaaat
     1141 agggctttga caaaaacgtg tcacagccct atttcgatta aacggtgtgt acctgtcaac
     1201 tcttcggtca caagtcataa tgttcataaa catgaggagc tgttttttga aattgttcct
     1261 tgtgaatcag agttaaacga caggcttacg aactcttgat taaacggcgt tcaaaagctt
     1321 gcatacgcgg taataagaac agccaggtca aaaacatgag aagccgtttt tcctgggctg
     1381 ttcttattac ttgatgcagg gcagcttttt cacaacctct ttattcagat aaaaccaagc
     1441 ttggatcagc attcaactga tagctagcaa aatttctttt ttgcatttcc acatgtgctg
     1501 ctgctccaat cattgcagca ttatctccac aaagtcgaag agggggaatc actagttcta
     1561 cctcagggag tttagttgcc aaagcatcct gcaatccttc acgcaaccct tgattggccg
     1621 ctacacctcc agctataatc agttgcttga ctggataatt ttgacaagct cgaagtgttt
     1681 tagtaatcaa tacatcgatc acacttgctt gaaagctagc tgctaaatca tttttgtcta
     1741 gatcttctcc acgctgttgt gcattatgca ccaaattgat aaatgcactt tttaatccgc
     1801 taaaactgaa atcataatta tcttcatgga tcatcgctcg cggaaaatgg taattatctt
     1861 ttccctgatg agctagctga tcgatttctt ttccgcttgg gtaagaaaga cctaatactc
     1921 ttccaacttt gtcataagct tctcctgctg cgtcatctct tgtctcccca atgatttcat
     1981 atgatccatc ttcttgcata tagactaatt ctgtatgtcc tccgctaaca agaagtgcca
     2041 tcaatggaaa ctggaatggt ttaaccagac gggctgcata gatatgccct gccatatggt
     2101 ttacaggaat aagcggcaat tggtgtgccc aagcaaaagc cttagctgca gaaataccaa
     2161 tcagcaacga gccgacaagt cctggtccat aagtgacagc gactgccgat aaatcttctg
     2221 ccgacacacc agcttctacc agtgcatctt ccaagcataa ggtgatttgt tctacgtgat
     2281 ggcggctagc tacttcaggt actacgccac caaaacgttt atgactgttt atttgagaag
     2341 caacgatgtt agataagatc tctgttccat ttctaacgac cgctacactc gtttcatcac
     2401 aacttgattc aatcgccaga atcaattctt cattcatttc atcgtctccg tttttaactt
     2461 tctgctcata atgatcgcat cttccgtcgg attgcgataa tatgatttcc gtttacctaa
     2521 tatacgaaac ttttctgatt cataaagttt tcttgctgct tcattagata gacgtacttc
     2581 taaaaaaaca tgcagcgaat cttttatttg ttccgtaatc agtaattgta acaaatgccg
     2641 tgcatgccct tttccttgtt ctgaaactgc aacagcaata tttgtgatct cgacttcatc
     2701 tattactttc gagtatccaa taaagccaca gatctggtta tcttcagcta aaagcagata
     2761 atcagagtga ggctgttgga tgtctgccaa aaactgcatt ttcgtccaag gtgaaccatg
     2821 tggataagca ttttcgctaa tcatccatac gcattctgcc aagtacgctt catcaaaatc
     2881 tttttttctg caaatcaact aatctcactc attttctaaa agttttatca tatccaacgc
     2941 atcttctttt gtttcagtgt aatatctttt tttgatcgtt gttgcttgaa accccaattt
     3001 ccgataaagt ctttgtgcat cttgattact catacgcact tctaatgaca tcgtctcaca
     3061 gcgattcatt atagcaaagt tctcgatttc gtcaataagc agcgaaccga ttcgtttacg
     3121 ctgatatgcc ggtcgaacgg caatattcgt gatatggcaa tctaaaccga acgctcttga
     3181 accgataaaa ccaatcattc ttccattatc aaagatacct agatatagat gaggaagcgg
     3241 tgacttcaat tctgatataa aggcactttt tgtccatggc aattcgccat tatagacttc
     3301 tcgttcgaca tccagcagct ctttgatatc ttctgtagtc aattctctta gtacgtaacg
     3361 acgatcgatt tgttcgactg ttttttctgt ataaatcatt ttatcttgaa aaaagcttct
     3421 ggcatacgct ctaaattttt tcaacataac tttcgtctcc aggtgtgtgc gtctccaacc
     3481 atttttcttc tgcttctact cgttttaaat agcgaggaag aaaagcttgt ttctctgcaa
     3541 ctggttcaac taaagcacct aattgagcca cagttacacc acttggaata tcccatgctg
     3601 gaactgtatt aatcatcgca ctaggcaata tttcctttat ttcagagaag aatttgtgac
     3661 aatcactccc gacgaaataa atagcttcat tcagcttttg tagttgtgtg aacaaatcag
     3721 aagcagaaat atgtacatct tcaagtacag tttctaatct attttctttc cagcggtatg
     3781 ccccagcgta tacatttttt cttcgagcat caaacatggg cacaataata ttatccacac
     3841 cgatgcaatt agccgcgatg gttttcagac tagatactcc tactagctct ttatctaatg
     3901 tataagcaat cgtttttgct gtcgttacac ctatacgaag ccctgtataa gaaccgggac
     3961 catcagaaac cgcgattcgg tcaatgtctt ttggacttat ttgaagatct gcaaacaatt
     4021 ggtcgattgc aggcatcagt gttacactgt ggttgcgttt gatatttgtt tgtatttgtc
     4081 caagaatttg ttcgtcttct accacaccga tcgccaaagt ttggtttgca gtatctattc
     4141 ctaatgtaat catatatttg atcctttctg ctatgacatt ctcgccttcc attgtagcat
     4201 acttttttat tctgaggatg aaaataccaa aggcaaaact agtacaataa tacatgatta
     4261 tctgctggcg ggcaaatttt gccccttcct gaaacacaaa aaagctgcgg tatcactcca
     4321 ttttcccaca gcttttccat cttttatttc gttgattctc gaataatcaa ctctgtttcc
     4381 acaataatct ttcttgaaac cggtgctttg gaattactta attgaagaat cgtctccact
     4441 gctagctctc ccatccattc ggtataaact tttacagtag aaagcgaggg cgttacatat
     4501 ttcgctacac tgacatcatt gaagcccatc acagaaatat cttctggtac cactaaacct
     4561 ttttcttgaa tcgctttcag tgcgccaatc gctaatgcat cattagctgc aaaaagcgca
     4621 tcaggaacat gcggattcgt ttttaaatac tgcttcactg cttgatatcc tgactcgaca
     4681 gaaaaatctg cttcgataat tggaccttcg tcgtagcctt tttctgccaa aacttgttga
     4741 aatgccataa ttctgggatc ttttagccgc tgatggtttt tcttcgtata ttctgttcca
     4801 gcaagcatag cgatatgttg cgctttatgg gagacaaagt gttcaatgac tttttgaaca
     4861 cttgacttaa agtcgacaac tagagagtcg attccttgag aagtgccgtc agaatctacc
     4921 aaaagcaagg cattttggga tcctttcatt tctaataact gagcttcgtc gaattttcct
     4981 agtgcgatcg ttccatctgc cggaagatca ctaatggaat cccacgtttc tttcaataaa
     5041 gtaacgtttg cttcttctgc ctttttttcg attcctaggc gaatagataa ataataaaga
     5101 tcttcaagct cttccgtatc gtcgtaccac tggatcaggc gaagcgtttg cttagtattt
     5161 gctgcatttt tacgatgttt cgtataattt aagcgttctg ctgcttcaaa aattttttgc
     5221 tttgtggcta agccaacgga aagatcagga tcatagttga gaactcttga aacagtagca
     5281 ggagaaactc ctgccaactg cgcaatgtct ttaattgttg ccatttgtct ctcctccttt
     5341 tttacgttta taaaatcgca gtaaatcgtt caaacgctgc ctgtcctttt tcatctcgct
     5401 tgaatactcc agcgtcttcc agtacacgtg caaaaacctg acctacagat ttttgtacga
     5461 catcggtgac cgtatctttc gtcaaatcag gatgttcttt ttttaattgt tctgcccacg
     5521 gacgatgata ttcagccatc tggttttctt ctcctaataa gaaatgctct acttctacta
     5581 gttctggttt caaacgaggt ggtaaaacag caagccccat tacttcgatc aacccaatgt
     5641 tctccttttt gatatgttgg acatctttat gtggatgaaa aatcccatca ggatgttctt
     5701 ctgaaacatt gttgtctcga aggacaaggt ccaactcaaa gtaattgtcc cttcttcgtg
     5761 caattggcgt aatcgtatgg tgtggtgtac catctgctga aaaagctctg atagatactg
     5821 tttcatccga atatgctttc catttttcca atactgagtt agctgcttca ataagcaagt
     5881 ttttctctgt tgattgcaaa cggatcacag acatcggcca tttcacgact cctgcgatga
     5941 cttgcggata atcttctagt ttgataagtt tttctatcgg tgcttttgcc ataggaaatt
     6001 catgacgtcc tgcctgataa tgatcatgag aaagaatcga tcctccaaca attggtaagt
     6061 cagcattcga accaacaaag taatgaggca agacttcagt aatccgaagc agtctagcaa
     6121 atgtttcctt tgtgattttc atcgggcggt gttgttctga taaaatgatc gaatgttcat
     6181 tgtaataagc ataaggggaa tattggaatc cccagctttc accgtctaga ttcattcgaa
     6241 taatccggtg attggtgcgg gctggatagt tcaagcgacc tctatatcct tcgttttcca
     6301 tgcaaagcat gcaggtagga tagtctactt ttaccgcctc gcgttctgct gcgatttgtt
     6361 ttggatcttt ttctggctta gataaattga ttgtgatttc cagttcacca tactccgact
     6421 ctgccggaaa tttgatgttt tttgcgatgg ctcttgtttt gatgtagtca tttttcttac
     6481 ttaaattaaa gaaatactca gttgcttcct ctgggctttt cgaatagtgc tgtgcaaaga
     6541 atgcattgac gactgaaggt ggcggtgtga gaaaatccat taattgcgct tcaaaaatct
     6601 cttgttctgc tggcaattct gaaagaacac cattttctct tgccttagcg acaagctgat
     6661 ccaataagtc tacagattct tccttaactg gtcttacttc aatgctttct agagcatctt
     6721 ctcctaccat ccctaaaaga cgattctgta aatataagcg gtccatttcc atccatccgc
     6781 cagcttcgat cgcaagtgtg gcaaaatcac aaatcgtttg actgattgac atatctcatt
     6841 cccctattct tcataaccat ttggatggct gacatgccaa tcccatgctg ttttgataat
     6901 cgctttgata tctgtgtatt caggtttcca gcccagaatc tctctcgctt tttcacttga
     6961 agcgactaat cggctaggat ctccagctct tcttggtgct atttctgctg gaatttcttt
     7021 tcctgtcact tctctagctg cttctagcat ttccttgact gagtatcctt tgctgctgcc
     7081 taaattgaag aagttgcttt catttccttc tttcaagtat tccaaagcta aaatatgtgc
     7141 agcaatcaaa tcttctactt gaacataatc tctgatacat gttccatctg gtgtatcata
     7201 atcgtctcca taaacagcca aggcttttct ttgaccaaga gctacttgta aaatgattgg
     7261 gacaagatgc gtttctggtg tatgatcttc tccgatagat gcatctgctt ttgctccggc
     7321 tacattgaag tagcgcaaag cgacatagcg catgccatat gcttggtcac accacttcat
     7381 cattttttcc atcatcaatt tactttcacc atacggattt ttaggattag ctggtgtatc
     7441 ttctgtgatt ggagactctt tcggctctcc ataagttgct gcagtagaag agaatacgat
     7501 tttattcacg ttgaactcgt gcatgacttc aagtaagatt tgcataccgt atacgttgtt
     7561 gttgaaatac atcaatggtt tttcgacaga ttctcctact aaagagcttg cagcaaaatg
     7621 gattactcct tcaatcggtt ccttttcaaa tacggaacgt agaaattctt tatcccggat
     7681 gtctccttca taaaaatgag catctggatg tactgcttgt ttgtgacctg tgagcaaatt
     7741 atctaccaca gctacttgat accccttttg aactagctgg tctacagcgt gcgaaccgat
     7801 ataaccggct ccgcctagta ctaaaattgt cataaaaaac actcctttga ttagtaaaat
     7861 tgtgtttgtt gattc
//
LOCUS       DAFFSV010000122         7841 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFFSV010000122 DAFFSV010000000
VERSION     DAFFSV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.122"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            172..690
                     /locus_tag="LR110_002403"
     CDS             172..690
                     /locus_tag="LR110_002403"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL4823344.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            818..1501
                     /locus_tag="LR110_002404"
     CDS             818..1501
                     /locus_tag="LR110_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBL4823345.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1810..1883
                     /locus_tag="LR110_002405"
     tRNA            1810..1883
                     /locus_tag="LR110_002405"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1844..1846,aa:Arg,seq:tct)
     gene            2042..2401
                     /locus_tag="LR110_002406"
     CDS             2042..2401
                     /locus_tag="LR110_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBL4823346.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2524..4155
                     /locus_tag="LR110_002407"
     CDS             2524..4155
                     /locus_tag="LR110_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBL4823347.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4217..4858)
                     /locus_tag="LR110_002408"
     CDS             complement(4217..4858)
                     /locus_tag="LR110_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL4823348.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5044..5120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5140..5448
                     /gene="rplU"
                     /locus_tag="LR110_002409"
     CDS             5140..5448
                     /gene="rplU"
                     /locus_tag="LR110_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBL4823349.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5475..5819
                     /locus_tag="LR110_002410"
     CDS             5475..5819
                     /locus_tag="LR110_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBL4823350.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5832..6125
                     /gene="rpmA"
                     /locus_tag="LR110_002411"
     CDS             5832..6125
                     /gene="rpmA"
                     /locus_tag="LR110_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBL4823351.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6218..7366)
                     /locus_tag="LR110_002412"
     CDS             complement(6218..7366)
                     /locus_tag="LR110_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL4823352.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(7378..7689)
                     /locus_tag="LR110_002413"
     CDS             complement(7378..7689)
                     /locus_tag="LR110_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823353.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
BASE COUNT         2376 a         1276 c         1549 g         2640 t
ORIGIN      
        1 gttttttaaa ctaacgtcaa ttatttataa taaaaaatga gataaagatg aggaaataga
       61 attagaaaca tataattatt aaagggaaac atttttacag ctataatttg aaaaaaattt
      121 atcgttatta tatgattaag aaggattgaa taaagaaaga agttgaaaac gatacctgat
      181 tacaaaaaaa taacttttat aagttctata acgttgatta tatttttaat tcttattctt
      241 ctcgtcgtct tggttccccg aatttttggt ttgactcctt atattattga ggacaaagaa
      301 atgagtccat attatgcaca aggaaatctg atatatgtac gaccgaaaaa tacaaatgag
      361 attttagtcg gagatattat tacatattat gaaaattcag gaaaaaaaat ctcgacgcgt
      421 cgcgtaatag ccgttgaaaa caaccacgaa gatttttata caaaagctga tacaaagact
      481 tatattgagc ctggagtagt aaaaaagaga aatattatcg gtagtcccat ctttcagatt
      541 ccatatattg gactagtcct ttcaaaaacg gcatttgcat ggattcaggg gttattcttt
      601 acgattgctt tttgcctaac aggaatcaca gcatggaata cctttgtaga gttgaaaaag
      661 aagcggagac gaacacagaa atttttgtaa gaatgttaaa aacataaatg tattccagag
      721 atccaacgac tcttctgaac atgaaacacg agaaaacact catgcagctt gtagatttag
      781 tcagttgttc gtataatgtg tgaagaggag gagtagtatg cgcaatgaaa tgatgcatcg
      841 tcctgtagtc aaagaagaac tattggattt tatgcgtacc cagcaaaaaa aattaccggg
      901 tgagctaggc aaactggaag aagaagctca tgtagcagaa gtaccagtta ttccccatga
      961 aacagttgta tttttgaaat ttttacttgg acagcttcag cctgaacgaa tcttggagat
     1021 cggagcagct atcggttttt catcaagtgt catggccacg acaatcggag aaaacgctca
     1081 tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa gctaaaaaaa actatgaacg
     1141 tctaggactg acagataaag tgactttgct tgaaggtcaa gcagcagata tcttgccgga
     1201 gttgtctggt ccgtatgact ttatttttat ggatagtgca aaatcaaaat atattgaatt
     1261 tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt ttgatggtag atgacatctt
     1321 ccaaggagga acgatcttgc tcccagatga agagattccc cgtggcaaac gagctatcca
     1381 tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat cccgatttga catcgacaat
     1441 cttgccatta ggtgatggtg tgatcttaat gacaaaggaa tcagaaacaa tcgagttgtg
     1501 atacatgatt atggcagcct gtagataatc gattgaatcg agggctcaat tcgaacttga
     1561 tgtaaaagtt tctatatttg taagtcaatt aaaggataca ggaaatctaa ataaacagga
     1621 gtagcatgaa gtcaatgtag ctcctgtttt caattgataa ccttagaaca gctgaaggag
     1681 aatgtctcgt tttaaaagat tgatttagta gttgggccaa aataattcat tatttgctaa
     1741 ttaaataact atttcgtatt gacagaagca atttaattat ctataattat tgatgttccg
     1801 ctgattcttg tcatagtagc tcagctggat agagcatccg ccttctaagc ggacggtcgg
     1861 gggttcgaat ccctcctgtg acgctaaaaa accgttagaa actgaggttt ctaacggttt
     1921 ttctttgttt tttgtgccaa tattgtttgt tttcaaaggc aaataaaata aaaaaacatc
     1981 cttatcgagt acaattataa ggagcaaaaa aataaaactc atcagaaaag aggaagaaga
     2041 tatgacgata ctatctagaa ttttagtgac tctagttgca attgaatttt tttatatcat
     2101 gtatatcgaa acggtgagaa ctgattctga cacgacaagt agggttttta aaatgagtaa
     2161 agaagagtta agcagaaaat ctgttcaaac attgtttaag aaccaaggag tgtacaatgg
     2221 actattagga gtgggactac tttatggagc ttatctttct tccgcatcta aagaaatcac
     2281 aagtatgcta ttaatatcta ttttttttgt tgcattatat ggcagtttga ctagcgatcg
     2341 ttggatttta ctcaaacaag gtgggctagc tctgttggca ttgctttcta ttttcttata
     2401 aatatagaaa aaataagaat ttatctaaca ggaaactttg tgaaaaatat tactttatct
     2461 catgaaatat gatattctaa tgaatggtat tttagaaaat ttcatgaaaa aggagttaga
     2521 taggtgttca aaaattatgt ttcctcacta aaaaaagaat tttcaggtta tgatctttca
     2581 agatttcaga aagatctttt ggctgggatc acagtagctg ctgtagcatt gcctttagcc
     2641 ttggcttttg gggtatcaag cggagctgat gcggccgcag gtttgattac ggctatcatt
     2701 gccggtatcg ttattggcgg tttatcaggc ggtttttatc aaatatcagg tccgacaggt
     2761 gcgatggcag ctatcttgat gtctattgcg gcaacaaagg gaatgcaagg tgtattgctt
     2821 gctacttttt tagcgggtct atttttactt gcagcaggac ttttccgatt aggaacactg
     2881 acttctttta tcccagcacc cgtaattacg gggtttactt caggtattgc tatcattatt
     2941 gcattaggtc agatcgataa tttatttggt gttcactcgg aaggtacaaa cgttattgaa
     3001 aaactaagta gttatcaaac gataggattt ccgataaaca cagcctcttt gatcatggga
     3061 gggctagtca ttttagggat gtttatctat ccaaaaaaat gggcgaaacg aatgcctagt
     3121 tcgctgctgg ctatcatttt ggcaacagca gttatgctat tgttccattt accagtagca
     3181 actgtgggta agattccaca aacattgatc agtagtaatc gtttgaatat gggagatttt
     3241 agcttttctg ccttgcaaca agtggcagtt ccagctatta gcattgcttt attaggaatg
     3301 attgaaagct tgctatgtgg tgcttcggct ggccgaatgg caaataagcc cttagatagc
     3361 aatcaagaat taatggcgca aggaattgga aatctgttgc ttcctttctt tggcgggatt
     3421 cctgctactg cggccatcgc ccgtacaagc gtggccatca aatctggtgc acaaactaga
     3481 ttagctggta tgatacatgc agttgtctta ctgctctcca tgcttgtttt tgcgccagtg
     3541 atgtcacaga ttccaatgcc agcacttgca ggtgttttga ttgtgacggc ttggcgtatg
     3601 aatgaatggg aaacgatcaa ggagctattt actaataaat attggtcagc agttctattg
     3661 tttatcctta cgatgatttg tacagttatc tttgatttaa gtattgctat cgtgatcggg
     3721 gtgatcggcg gctgtgtatt ttttgttgtc aaaagcgctg ctatcacgat ttcattagaa
     3781 acgatcgatt ggcaaagaat ggggctgcca gaaacggaaa agctgaataa ctggacagta
     3841 gtgtatatta gtggtccact gttttttatg tcttcagaaa aactcaaaaa taaattggaa
     3901 gaactaaatc atcacgatgg tgtcattttc tccatgcgag gcgtaccaag tattgatttg
     3961 acggcacgtg cagtttttga tgaatttcaa gaaaaagcag agcaaagagg acaaacgatt
     4021 atctatactt ccttgcagcc agaagtagaa aaacaacttt ctcacttgtg ggaacataca
     4081 ttcacgcatc aacatcaaac agttgctcat gcgttgacag ctttacatgc gaaatatact
     4141 gcaattagcc attaaaacaa tgaaaaagaa cagacaagac tgtaaactga agattttatg
     4201 gtcttgtctg ttctttttaa tattgctgta aaagtgaagc tagtacttgc cagtcttgat
     4261 ggataacagg tacaagggag cgattgtaga aaatagctgc tggatgaaaa agtggaagaa
     4321 ttcgatagtt tttttctgac cattcgtagg catccttttg ttctgttaat tgtaagacag
     4381 gccctgtata gagttgacca tgatttttgg taaccgtata ttctttgcca agtaaacgtt
     4441 gaagaccaat gtttccaagt gtcgcaatta attttggctg gaccttcatc agttcgtaat
     4501 ccaatattgg tgcatgcgca aatacttcgg aacgagtagg tgtccgattt ggataaacag
     4561 tttctgactg ttgtgtccgt ttattgatcc gatgagtcac gcgatacggg cggctcctta
     4621 cagcactggt gatatacacc tcttctcgtg ttaagccagt tgaagaaagt gcctgcatca
     4681 attcttttcc agcttgaccg ctaaaaggaa catgcgtatc aatctctgtt ttccctgggg
     4741 cttctcccac gagcatcagt ttaggatgaa gaggtccttg cccagcgata aatccttcta
     4801 caggacggcc gttcattctt aaagaagctt cttgtatcag ttcttttggg tattccattt
     4861 atgctcctcc tttttctttt attataagaa taaaagaaac acaatgcgac tattttattt
     4921 ggtagaactg tgaggataga aaagcagaag tattgcttga atttttgttt tactttttga
     4981 ttttaaattt agttgactaa cggttgctgt tctggtaagc tagtactgtt gtaattgtgg
     5041 actccacagc tacaaccgct cagaacaagt aataagcaca gcatatgctg tcacttcgga
     5101 tggcgagtct aagtatatca gaaggaggtg ctttatagca tgtacgcaat tattaaaact
     5161 ggtggtaaac aagtaaaagt tgaagtggga caagcaatct atgttgaaaa acttgacgtt
     5221 gaagcaggag aaaaagttgt ttttgacgaa gttatcttag taggcggaga atctacaaaa
     5281 gtaggcgctc caactgttgc aggtgcaact gttgaaggta ctgttgaaaa acacggcaaa
     5341 caaaagaaag tcgttacttt caaatacaaa cctaaaaaac attcacaccg taaacaaggt
     5401 catcgtcaac catatacaaa agtggttatc gacgcgatca acgcttaata gcgtttgaga
     5461 aagaaggcct atatatgatc aaaggaacat ttaagcgaaa tgacgcaggt cagatcgttt
     5521 cattcacttt gaccgggcat gctgatgctg gtccttatgg aagtgatatc gtttgtgcgg
     5581 gtgtttctgc gttagcgatc agcactgtca acgggattgc ctcattagcc ggttttgagc
     5641 cgattgttga aatgaatgaa gaagaaggcg gttatcttta taccgaagtc acttcaggaa
     5701 tgacacaaga acaaaacaat attgctgaga ttctattaga aaatctttta ttaggattac
     5761 aatcgataga agctgaaaac tcagaatata tccaaattaa aacaattaat gataaatagg
     5821 aggtgcagac catgttgtta tctatgaatc tacaattatt tgcccacaaa aaaggtggcg
     5881 gttctacatc taacggccgt gactcagaat caaaacgtct aggtgctaaa cgtgctgatg
     5941 gacaaacagt tacaggtgga tcaattcttt accgtcaacg cggtacaaaa atctatccag
     6001 gtgtaaacgt gggtatcggt ggagacgaca ctttatttgc aaaagttgat ggcgtagtac
     6061 gtttcgaacg taaaggccgt aacaaaaaac aagtgtctgt ttatccagta gctcaagaag
     6121 cttaataaat aaactgctct atctcttgtg tagcaaggga tggagctttt ttgtaaatta
     6181 tgggattttt tttgaccata cttttgacca tactttctta gaagttaata taatttgtta
     6241 gtttttgtgt tgtttcttct tttttgctgt tagtaatgtg tgtatatgtg tccatagtag
     6301 tttttatatt tgcgtggccc attctttctt ggacttcttt gtgatctgcg cctgcctcat
     6361 atagcattga tgcatgtgta tgtctaaaac catgcagacc gatataaggt aagccggcac
     6421 gctcacgata tatattgtat ctttctgcca ctgactgatt aattgtgaaa ttattttcgt
     6481 cattggtaaa caaataatta gctttagtaa atcctaattg aaataagtac tttctttgtt
     6541 cgagcttcca aaattttagt tgctttatag ttttttcatc taaataaatt attcgatttg
     6601 attttttggt tttaggagtt tcagatatat aatatttatc tgaacgagct acagtttttt
     6661 taatggccat ttcatttgtt ttaaaattaa tattgtccca agtgagtgct aatatttcgc
     6721 cgattctaca tccgctgaat gctaatagtc taaagagcgt atagtctctg tttttaaaat
     6781 atggattctg ttcttgagat acttcattta gaaataattc caactgttct tttgtatata
     6841 attttaattt cttttctgtt ttaatttgtg gctttggaat aagtaaattt aatgttggat
     6901 tatcagatgt taaaccaata tttatagcat acttaaaaac tttattcata tagtttatga
     6961 aaagagggta ctgctttgaa gttccttttt cactccaatc atttactacc ttttgacaat
     7021 atgccgtatt aattttagaa agcttaatat ttccaaattt gggatatata tgtttattaa
     7081 agattatttt agtccttgaa tatgaacttt cttttacagt ttttttgtag ctttctagcc
     7141 ataattctgc tgcttctttg aaagtagtat ttgtacttgt gggcattcct gttttttgta
     7201 attctagttc taacctagaa agtgctgttt ttgcttcttt ttgtgttcta aatcctcgtt
     7261 tagtagtata ttttttcttt ccagttagcg gatcaatacc gagataagtt ttaaagtacc
     7321 atgctttttc gccatttttc tttttatatt gttttatcat tgccatcaat attaccccta
     7381 ttcaaattct aattctgcca tgctatcatt gaaaaattcg atcagtcctt gttcctctat
     7441 aaattcaagc catatttcct tcccgttaat taatggaact cctttttctt tacgaattgt
     7501 ttgatacaag ttagtcacga cttgtttcca catttctaat acagctttat ctattttctt
     7561 atcatcccag cgtttttttt ggggtctatt tttgtttttt tcaatttgat aattagtagc
     7621 tttgatgata aatgtttcgt atccggttgt gcttttttct aaccattctt tgaattctgt
     7681 atatatcatt tttgttcacc tttctatttt tgaacgtatg ttcttttttg cttaaaaaat
     7741 tttagagagc tgcataacaa tagatgcgag cttttaataa ttctttcaca agtggttcga
     7801 agcgaacgct taaattgtta tcttctataa atttcatgta a
//
LOCUS       DAFFSV010000123         7776 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFFSV010000123 DAFFSV010000000
VERSION     DAFFSV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.123"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            213..1520
                     /locus_tag="LR110_002414"
     CDS             213..1520
                     /locus_tag="LR110_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBL4823354.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFDNLF"
     gene            1639..2838
                     /locus_tag="LR110_002415"
     CDS             1639..2838
                     /locus_tag="LR110_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL4823355.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(2865..4133)
                     /locus_tag="LR110_002416"
     CDS             complement(2865..4133)
                     /locus_tag="LR110_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBL4823356.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLITLTL
                     FGTITTHLFGGSVGREGTAVQMGGSLADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(4296..4943)
                     /locus_tag="LR110_002417"
     CDS             complement(4296..4943)
                     /locus_tag="LR110_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HBL4823357.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILNHELPEIDTFLTTVLDVKLTKAQAERALEKLKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(5351..5854)
                     /locus_tag="LR110_002418"
     CDS             complement(5351..5854)
                     /locus_tag="LR110_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL4823358.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(5977..6741)
                     /locus_tag="LR110_002419"
     CDS             complement(5977..6741)
                     /locus_tag="LR110_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBL4823359.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSADQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            6848..7123
                     /locus_tag="LR110_002420"
     CDS             6848..7123
                     /locus_tag="LR110_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBL4823360.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         2337 a         1606 c         1421 g         2412 t
ORIGIN      
        1 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacacg
       61 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
      121 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
      181 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
      241 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
      301 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
      361 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
      421 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg gatgataaag
      481 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
      541 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
      601 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
      661 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
      721 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
      781 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
      841 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
      901 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
      961 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     1021 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     1081 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     1141 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     1201 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     1261 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     1321 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     1381 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     1441 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     1501 ttttcgacaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     1561 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     1621 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     1681 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     1741 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     1801 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     1861 ttcacagaaa aacgaaggct gctgaatggt cttttattca atgttttcct catcagtttc
     1921 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata ttttccttgg cggactgatc
     1981 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     2041 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     2101 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     2161 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     2221 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     2281 cgatacaatc aagacttcat cgttgtgtta ggcgctgggt tgatcaatgg agaaacagtt
     2341 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     2401 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     2461 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     2521 cttgtcgaaa ccaattcgac aaccactttg gagaacatgc tctattcaaa ggaaatcatg
     2581 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     2641 agagccggtc tttatgcgtg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     2701 gctttctact acttgcctaa tgcgttttta agagaataca tcgccatctt gatgatgaac
     2761 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     2821 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     2881 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctgg
     2941 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     3001 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     3061 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     3121 tgaagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     3181 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     3241 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     3301 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     3361 cgccaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     3421 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     3481 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     3541 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     3601 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     3661 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgctttatcc
     3721 aatcggaaca aacgtgctgt attatctgct aacgatcccc ccatttggac tgctgttcct
     3781 tcacggccaa cagacccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgta
     3841 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     3901 ccacgactag aaagaccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     3961 ggtagtagat aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     4021 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     4081 cacttcagca tgtacactaa aatgcgcatt ggactcggta attgtttttc cataaaaaat
     4141 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     4201 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     4261 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     4321 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     4381 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     4441 aaaagcgaca cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     4501 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     4561 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     4621 gctggttgtt tgagtttttt tacttttcgg aataatttct cgatttgtgg tttgctaggc
     4681 tgcataaacg gctgaacgat ttgcttcagc ttctccaatg ctctttctgc ttgcgctttt
     4741 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga tttctggcag ttcgtgattt
     4801 aatatgtctc gaatcttttc agctgctagt gcctgaactg tttgtaccgt ttttggatca
     4861 ttgacagatg agtacgcatt taataactgt tctacttctc tttttataaa aaaatattgg
     4921 tatggtttta ttgtttgttc catggtttta aagtcctttc ttctaaccaa agtgcatact
     4981 ttgtagaaaa aagtgatctt ctgcaaagta tgtgttacct gaagatcccg gactctttta
     5041 aataagatac gttttgcatc ttatcatcac cccctttaat gcttattctt ctatgtacat
     5101 gattcattcg ataaataaaa gtaaaccgta tcttgaacag atagtcaact gcaaacagaa
     5161 aaaaggactg tgacaaaagt cgtgtcaaag tcccttgttt cgaatgaatg gtgttcaaaa
     5221 gcttgcatct gcggtaataa gccgaaactt cacaaaaatt tgagaagcaa ttttcgtaaa
     5281 ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcaatatgg tctattattg
     5341 gttttacctg ttaaatcgag gattctttcc caaagtaacg ttgccatctc ttacgcatac
     5401 ttgcagacat gggctgagcc gcttctttga ctgcgcagta tttgtccacg aatgtcacaa
     5461 tatatccttc tttatactta ggcggtgcga tcgttgcacc ccacatgtgc ttcaaaatga
     5521 tatctcgctc aagatccgac aaatcggtga ttttttctgc attctttaca gcaattcttg
     5581 gatggatata cgcatgtgtc ccctcgtcaa atttcgtcgt acgccaatca tagtagaaca
     5641 gatcatgcaa aagtccagca cgcgctgttg ctttagcatt gccaccccat ttttttgcga
     5701 gcagataact atagtaagaa acactgatgg aatgctccaa acgagtggaa tgaacatgct
     5761 gtgtgtaatt cgctaatttt ttcactgctt ctgtttctaa aagatcttgt acataagaca
     5821 tatattcttc gtcttcacgc catttttctg tcataattat cgatctgcca cctttttgaa
     5881 tttcattttc ttctgaaatt gatctaagta taccaccagt gttcggatta ttctagtcgc
     5941 tctagataaa atagctaaag cttaatagtt ccttaatcat aaagaaaaat gataaagaac
     6001 gatccctgct gcgactccga cattcaatga ttccgcattt cctttcattg ggatatatac
     6061 gttctgatca gccgaagaca agatttcctg acttactccc tgaccttcgt ttcccatgat
     6121 tatagcgaaa tcctctgttt tttctacttt gtctaatgca tgtgcttctt catttaattc
     6181 tgtaccgtac accgtaaacc ctttgccttt cattacaggg atcacttctg ccaaatcttt
     6241 ttgtaacaca ggcaaatgat aattgcttcc ctgcatgctt cgcagcacct ttgtactata
     6301 aatatctgcc gttcctttac ctaataaaac accagccata ccaaacgcat ctgccgtacg
     6361 gatcatcgtt cccacatttc caggatcctg tacattatct agtaaaagcc agcctcctga
     6421 aaaatctggc gtatcttcag atgttaacgt catcactgct aagatccctt gcggtgtagg
     6481 tagatctgac atagcggtca tgacctcatc tgaaactaga aaagtagaaa tctctttgtt
     6541 tacagccagc cagtctcccc attctttcat acctctctgt gtcacaaaaa gttcttttat
     6601 ttctgcatca gacgacgcag cttcttcgat cagatggaac ccttccaaaa gatattctgc
     6661 cttttcttct ctgtatttct ttttatgtag tttttttaat tctttgattc ttggattttt
     6721 tactgattgg atctctttca cattcctcac ctcaactgat tatatcatgg aataggtgct
     6781 tgagcttgtt tcatcctaaa aaaatcggta tcattgattt ataaaacagg aaagaaaggt
     6841 gacgaacatg cgcaaagtta aaatgaatgt ccaaggacgt gtccagggcg tgggatttcg
     6901 ctatatgacc aagatggttg ctgatcagtt aggcgtgacc ggaacggtaa aaaacgaaga
     6961 cgacggctca gtcacaatcg aagcaatcgg aaatgacgac atcatccaaa aatttatcga
     7021 agaagtcaag aagtctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     7081 tccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtt tttgaagcgc
     7141 tttgcctcga gtattaagga gaaacatttt ccaaatagct tctcatattt tgaaatgtaa
     7201 tgacttaata ccgagaggca gctgataaaa caccgtcaat aaggttgtga aaaagctggt
     7261 ctgcatcaag cattaaggac tggttgtgaa aaatagctct ccatattttt caacaggctg
     7321 gacttaatgc cgtagatgca agcttttgaa caccgtcgat aaggttgcga aaaagctgat
     7381 ctgcatcaag cattaaggac tagttgtgaa aaagtcgctt agttttgagt attaaggaaa
     7441 attttgtgaa aatggctttc cacatttttg cataaatttg acttaatacc aagaaactga
     7501 cttttgaaca tcgtcgataa ggttgttttt gaggcacttt gcctcaagta ttaaagacag
     7561 gtagtaaaaa agctgatcta taacttgcaa taaatttgct taacccatat tttccagaca
     7621 gaacaaaaaa gtttttagtt ttttaaataa gaaagaaaca cttggaatca ctcttttctt
     7681 tctaagtatt tctttcttga ttttttgtct tttttcaaaa tttttcgcta cgaaaaagaa
     7741 cgaatattta tatgaaattt ctatgaagtt gattga
//
LOCUS       DAFFSV010000124         7517 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFFSV010000124 DAFFSV010000000
VERSION     DAFFSV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.124"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(861..1274)
                     /locus_tag="LR110_002421"
     CDS             complement(861..1274)
                     /locus_tag="LR110_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBL4823361.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1730..2215)
                     /locus_tag="LR110_002422"
     CDS             complement(1730..2215)
                     /locus_tag="LR110_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823362.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2354..2599)
                     /locus_tag="LR110_002423"
     CDS             complement(2354..2599)
                     /locus_tag="LR110_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBL4823363.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2792..3592
                     /locus_tag="LR110_002424"
     CDS             2792..3592
                     /locus_tag="LR110_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL4823364.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3611..5479
                     /locus_tag="LR110_002425"
     CDS             3611..5479
                     /locus_tag="LR110_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL4823365.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5472..5963
                     /locus_tag="LR110_002426"
     CDS             5472..5963
                     /locus_tag="LR110_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBL4823366.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5951..6505
                     /gene="srlA"
                     /locus_tag="LR110_002427"
     CDS             5951..6505
                     /gene="srlA"
                     /locus_tag="LR110_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBL4823367.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6523..>7517
                     /locus_tag="LR110_002428"
     CDS             6523..>7517
                     /locus_tag="LR110_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBL4823368.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKNKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2672 a         1204 c         1432 g         2209 t
ORIGIN      
        1 ctttcaaaaa tatgttttgg aagttgggac aaacgagtta atacatttta atacattatt
       61 ttttattcaa aaaaaatagc acaatggcaa agatcgtaag agagatcata agccccaatg
      121 aatgactaaa aagaaaagca gaatcacatg ccatatcatc ccatatttct tcaagaaaat
      181 aaataagcgg gaaaaaggga aggaatagta gaacatcgat ccagcaccat ttttttcttc
      241 ttttgaaata acataaaaca ataaacatga acgtgcaggg aagtaaaaac aaagctttac
      301 tcataactaa caaaatacca gctcctttta tttttttctc ccccacattc aaagagtaac
      361 aatggtatgt acttaataat agaaaaatgg atcaacttgt atgaataaca gctgaaaaga
      421 tctgcaccaa ataaaaaact tccagccaaa actgactgga agtttatttt tttctgaaat
      481 attctaacac aaatatgaaa aagatggccg aagctaataa gccgatttcg tagcaaaaca
      541 atgagatacc agagtcgtaa taataaaatc ctctaatctt atggataaaa tagatcatcg
      601 gacaagcaga taagagtaag tatttgttag gccttttgaa taagtaaaag aaagcactgg
      661 tcattatgat aaatggtatc caaaacaaaa gtttacttaa gacaatcaaa tgatcaactc
      721 ccatattttt taccaagaga gatcataaca tctttatcgt aaaaacaaaa tatttttttg
      781 ctcattttga aaatttaaac atactcgttc ttatccaaat atataaaaat cgcccctcat
      841 tcatcgagag gcgataagtt ctaatttaac tcaatttttt ttgtttcttt tggctgataa
      901 gctgttttag gtaataccaa tttcagcaca ccgtttttaa aggttccagt gattttttct
      961 tcatcaacat taggaagata gaatgaacgt ttgtaagaga ccgaatgtct ttctttccgt
     1021 acatagttgc catcttcttt atcttctgta tgggactcat gattggcgga gatcgtcaaa
     1081 gtattatctg aatattcaac tgtcgtgtct tctttgtcac agccaggcat atcagcagtt
     1141 agtttgtatt ctttttcatt ttcgactaaa tctacttttg gataacttga aacacctaaa
     1201 aaatcattga atgctggact aaacacgtca ttgaaatcag ggaacatgtc gcgaatgcta
     1261 ggtacatttg ccatttttga tcatcctttc aataagtatt ttttatttat ccctatttac
     1321 gtaataactc tattttgaaa aaaatgcaag tataatggtt agctttgtga atatttcatc
     1381 taatacgata caaaggctat ctttctaatt tgaaaaaggg atcattaaaa taataagaag
     1441 aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca aatatagaaa aattgaatct
     1501 agaaaaccgc ccgacaataa atttcattaa ctcagttgac cgaaaatgaa gtagatacca
     1561 aaaatttatc aagagagcaa gtagagaagt gagtatattc ataagagaaa tagctgtaac
     1621 gaattattag gttaatgcaa atgcttattt atactccgta taactttctc agaaaaaatg
     1681 ataccaataa tgacttgcta gtactgtaaa tccgtataat atcatctaat caaaatggtg
     1741 ttccatttgc atctgtcatc gtaatcgatc cttcgggtga cactcgataa aaaccaacgc
     1801 ttccagatcc tccttgggaa cggatttgtt tgctggaagc tgtgaacaaa taatctcgat
     1861 caattttttg ataaaagcta ataacaatat cgtcactttg taattcggaa cggtgggcaa
     1921 taagaaatag aagtgctttg tcagagtttt ctaatggaag agaaggtaca gtgctttctt
     1981 gcatttcaga tgttgaagaa atgctaaacg actgaatact tgattggcta tgttgcgaat
     2041 ctacattacc agtagaagta ttagtaatgg aagtagtaga agtactttta gttgaaccaa
     2101 cctcagttgt tgaaatggct gctgaagatt ctttaacaga attctttgtt ttatatccac
     2161 aggcagaaaa aagtattaca gttcccaaca agcctgcgta aatcccttta tgcatgatag
     2221 ctcctcctaa aatagattat ctttattcag tatatcactt aaacaaagaa aagcagatgg
     2281 tttaaaatga aaaaaccttc ccaataaagg gaaggttcat gaagataata acttttttaa
     2341 taattatagc tttctaatat ctttcatcaa cttttctagt aatcattggg aaaaatttca
     2401 agcagtagat cgcacaaata cctacgacga tataaatgat cttcgcaaaa atagttgttg
     2461 aaccaccagc aatcatagct actaaatcaa gttcgaacaa gcctaccaac agccagttca
     2521 accctcccac aatcaataaa gttaatgcaa tagcatctaa gttttcatta gaattctcct
     2581 ttcttaaatg ataaaacaat tatatatcaa aaagagaaac attgcttata taacgctcag
     2641 gaattttagc aatctatgca aaaagatgaa aatttttttt gctcttccat tagtacacat
     2701 tttttagctg aagctgtaaa tttcatgtct taattttttt tagtgagctt ttatactgga
     2761 tttgtaagaa aaaagaagga gaggatcaca tatgtcggat tggttggata ttgcaggaaa
     2821 agtagtaatt gtaactggag gttcctcagg aattggaaga agtatcgtcg aaaatttgtt
     2881 gaagcagaat gcacaagtag cgaattttga tgtaactgaa tgccgtatac agcatgaaaa
     2941 cctactatct ttaaaagtcg atgtgtcttc taaaacggac atagaagaag ggatatataa
     3001 agtaatgaaa catttcaaga cgatcgatgg tctagtcaat aatgcaggaa taaatattcc
     3061 ttctcttcta atagatagaa atcatccaaa aagcaagtat gaattatcag aacaagtttt
     3121 tgataagatg atcgctgtca atcaaaaaag tgtatatttg atgagccaag cagttggaag
     3181 aatcttggtg caaaagggat caggagtgat cgtgaatctt tcttctgaat caggattgga
     3241 aggatcagaa ggacagagct gttatgctgc tacaaaagca gcgatgaaca gttttactcg
     3301 ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg gtaggtgttg caccagggat
     3361 actggaagaa accggtttga gaacacaaga atatgaagaa gctctttctt atacgagagg
     3421 tataagcgtc gaacagctga gaaacggtta cagtcgaaca agtacgatcc ctctaggaag
     3481 gagtggtaag ctgcaagaag tggcggatct agtctgctat tatctttcag aaagatcaag
     3541 ttatatcact ggagtcacga ctaatatttc aggaggtaaa acaagaggat aaaggatagg
     3601 ggggacgcac gtgacgctag taaacagatg gtaccaatta ttgaaattac tagtcactca
     3661 caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa accagtaatc aaaccataaa
     3721 aaaagatatt ggggagttga atgaagaaat tgccggtatt gcaaaaatca tccaaaaaaa
     3781 caaacatttt gaacttgaaa tttatgattt tgattttttt gatgagatca tgcaaggaaa
     3841 attgaaaaca gccagtgatt tcaattcggc atccaaaaga atcgcttatt tactaaatag
     3901 gctgatcgaa acagaggatt tcttacgtat ggatgatttg tctgaagaag tcggtgtcag
     3961 cagaggaaca gtggtcaagg atctaaagac attaaaagag atgatcaatg attttgatgt
     4021 aaagattaca ggcacaccaa ataaaggact aagaataata ggagatgagc tagaattacg
     4081 actactttta ttttattttg tttctcccta cttttcagag acgaaaacag aacagtttct
     4141 agaagaaaaa caagcaatca aacattttca gaagaagacg aatgctagta aacaagaaat
     4201 caccctttta acgaaagtta tcggtatttc tttgaataga atctctagta actatctgtt
     4261 gaagaagccc atcaatcgtt acagtgggtg ttttgaatat atccaagaat tcaatgaact
     4321 gatcaatcag ttggaagaaa tctatgaact aactttgagc caatatgaaa aagattttat
     4381 cagttttcct ttgaacatca tcaacacaag tataacgatg ataaggaaag ctaactctga
     4441 tttgcgctta tatttcgacc ccatgatgat gcgtattcga cgattagatc tcattgatct
     4501 ggatgagtcc ttcttatatc aagagatgaa acagcacctc tctttattga taaatcgggt
     4561 aatttttcgt tatcgattga ctgatttatt ttatggtgag atagaaaaaa agtatccttt
     4621 ttcttaccaa atcgctagcg aatgcataca agcattagaa gctatcttgc aacataagat
     4681 ctctaaagta gagatcagct acctaacgtt gtatttggag ttagcatttc gatatcaaaa
     4741 agagcatttt tcctctaaaa aagtagccat tgttcatacg acaggaaaag gaacagcatt
     4801 aatgatccaa agacaaatca aaaggatatt ggggagggat atacaaataa cacatgtttc
     4861 agaagaacaa tatgaaaaag tagatctaaa caaatatttc gctgttttta caacgattcc
     4921 gttaaaaaat gtaaatgaag agacaccggt gatccatttg actaatcttt ttgatgactc
     4981 ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga ttagccgggt ttcattctac
     5041 tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat caagaatatt taaacaaatt
     5101 agtcgctcct ttgatcagag gctattatgt ggatgaagaa tttgaaaaaa ggatcttgaa
     5161 aagagaatat cagcaaagca catttgttaa caaagaattt gcttttcctc acacaaccaa
     5221 ccagctatct caacacattg ttttatcagt agggctaata actgatcaag gtttagcaga
     5281 aacaggagga aatggagtta tttttttatt tgccatacct gaagaaatga cggctgaagt
     5341 tgagaatgaa ttatttgagc tatacgatat cctatttgaa ctcataaaaa tgggaataga
     5401 tgagtttatt gaattagcac cgacacaaga atcattttta aaactaatca aaagtaaagg
     5461 agagagactt catgaatgat atgtatctat tcatagtttt cgcagcttca gcttatgtgc
     5521 tgcaagcagt tatcagttgg aagcaactaa agcatttcaa tcaaacatat agcgaactgc
     5581 gaagaaaagg aaaagtagcg attggtaaac gacctgggaa aatccgttca ggcacgattg
     5641 ttatgctagc tatagacgat catgatcaag ttttagaagt cagaaaaatg caaggggtta
     5701 catctttggc aaaatttcat tcattacctg tctatgcggg tgaagatatc cattacatcg
     5761 atcgttacca tccctcagtt cgagcggaag atcgtttgac acaagcagct ttagaaaatg
     5821 ccagagaagt atacttacga atagaggcag gaaatgaaaa aagtgaacaa ccttcattgc
     5881 cttttattgc tatgctcaat aattttcgga tatggaaaag tacgatcaaa gaaaaaagga
     5941 gtgacaaacg atggatatca taacaaaatt tgctgaggga tttatgaggc tgttccaaac
     6001 aggtgcagaa acattcataa gctggatgac aggaatcgta ccagttgtct tgatgctgat
     6061 ggttgcaatg aatacgctga ttgctttttt aggtgaagac cgagtaacga aagtagccaa
     6121 attatcagcg aaaaatccgc ttactcgtta ccttgtgtta ccgttcttat ctgcttttat
     6181 gttgggaaat cctatgtcat ttacaatggc tcgtttcctt ccagaatact acaaaccaag
     6241 ctattatgct gctcaagctc aattttgtca tacgagcaat ggtgtattcc cccatattaa
     6301 tcctggagaa ttgtttgtat ggatggggat tgctcaaggg atcgaaacct tagggctaaa
     6361 ttcgatggag ctggctatcc gatacttgct tgtgggactg ctgatgaact ttattggtgg
     6421 ttggataact gattttacta ctggatttgt atgcagacag caaggaatcg ttcttagcaa
     6481 aaaagtagag ctttcagttg attagagaaa ggagagtttt aaatgtatgg tgatattaag
     6541 atcgtaagag gaaacggcgg gtatggaggt cctttgatta ttaggccgac aaaagataaa
     6601 aacaagttca tctatgtcac aggtggaggt gagaggccaa tgatcgtaga taaaatagaa
     6661 gaactgacag gtatggagtc tatcaatggg ttcaaaacat caatacctga tagtgaaatc
     6721 gcgcttgcaa tcgttgactg tggagggact cttagatgtg gaatttatcc taaaaaaggg
     6781 ataccaacca tcaatatcgt tcccacagga aaaagtggcc cattagcaca atatatcact
     6841 gaagatattt atgtttcagc agtagggtta gaacaaatac agccactaat ggaaactgat
     6901 gataacaaag aaacagtaaa tactgcatct ccagataaaa agtacaaata tgatacttct
     6961 aaaaagataa ccgagcaaaa agcagagcag caaaactgga tagcaaaaat tggaatgggc
     7021 gcgggaaaag tagttgctac attcaatcaa tcagcaagag aagcagtaga tacgatgatc
     7081 cggacagtga tcccttttat ggctttcgtt tcccttttga ttggtgtgat acaaggttct
     7141 gggatcggta atgtatttgc taacattatg gcacctttag caggaaatgc agttggatta
     7201 acaatcattg gctttatttg ttcactgcca tttttatctc ctttgcttgg tccaggggca
     7261 gttattagcc aagtgattgg aacattgatc ggtgtagaga ttgggaaagg aaatattcct
     7321 ccccaattag cattaccggc actatttgct atcaatactc aaaatgcatg tgattttatt
     7381 ccagtcggtc taggtttgga agaagcaaaa gctgagacag tagaagtagg tgtaccatca
     7441 gttctttatt caagatttct taacggcatc ccacgagtta tcattgcttg ggtagcaagt
     7501 tttggattat atagctg
//
LOCUS       DAFFSV010000125         7397 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFFSV010000125 DAFFSV010000000
VERSION     DAFFSV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.125"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..142)
                     /locus_tag="LR110_002429"
     CDS             complement(<1..142)
                     /locus_tag="LR110_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4823369.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="LR110_002430"
     CDS             complement(183..437)
                     /locus_tag="LR110_002430"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL4823370.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="LR110_002431"
     CDS             complement(548..802)
                     /locus_tag="LR110_002431"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4823371.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="LR110_002432"
     CDS             complement(995..1270)
                     /locus_tag="LR110_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBL4823372.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="LR110_002433"
     CDS             complement(1242..2195)
                     /locus_tag="LR110_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL4823373.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="LR110_002434"
     CDS             2807..4300
                     /locus_tag="LR110_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBL4823374.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="LR110_002435"
     CDS             4435..4731
                     /locus_tag="LR110_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL4823375.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="LR110_002436"
     CDS             complement(4755..5714)
                     /locus_tag="LR110_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL4823376.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..6748
                     /locus_tag="LR110_002437"
     CDS             5897..6748
                     /locus_tag="LR110_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823377.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6769..7052
                     /locus_tag="LR110_002438"
                     /pseudo
     CDS             6769..7052
                     /locus_tag="LR110_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2442 a         1199 c         1330 g         2426 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     6481 tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac
     6541 tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag
     6601 aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt
     6661 gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga
     6721 tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta
     6781 tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc
     6841 aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa
     6901 taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa
     6961 caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat
     7021 tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga
     7081 aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa
     7141 aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt
     7201 aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt
     7261 ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt
     7321 gatcgaagat gtgacgaacg gttctgttgc aaagttttaa atctactatc aaataaggta
     7381 gaataataga aaaagat
//
LOCUS       DAFFSV010000126         7338 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFFSV010000126 DAFFSV010000000
VERSION     DAFFSV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.126"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(38..190)
                     /locus_tag="LR110_002439"
     CDS             complement(38..190)
                     /locus_tag="LR110_002439"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL4823378.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(381..1259)
                     /locus_tag="LR110_002440"
     CDS             complement(381..1259)
                     /locus_tag="LR110_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL4823379.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1298..2416)
                     /locus_tag="LR110_002441"
     CDS             complement(1298..2416)
                     /locus_tag="LR110_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBL4823380.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2413..4512)
                     /locus_tag="LR110_002442"
     CDS             complement(2413..4512)
                     /locus_tag="LR110_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBL4823381.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4563..5384)
                     /locus_tag="LR110_002443"
     CDS             complement(4563..5384)
                     /locus_tag="LR110_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL4823382.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5381..6190)
                     /locus_tag="LR110_002444"
     CDS             complement(5381..6190)
                     /locus_tag="LR110_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4823383.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6211..6705)
                     /locus_tag="LR110_002445"
     CDS             complement(6211..6705)
                     /locus_tag="LR110_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4823384.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6717..7205)
                     /locus_tag="LR110_002446"
     CDS             complement(6717..7205)
                     /locus_tag="LR110_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4823385.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
BASE COUNT         2234 a         1691 c         1226 g         2187 t
ORIGIN      
        1 agaattacga tttattttat acggctgaaa aaatctatca ccaaaaaaat tcaaacatta
       61 agccaaggat tatgaagtca acatttcgaa aaatgaacat acattggtaa gcaactataa
      121 ctattaccac agacataaag taacacaata tattactatt taagtgtacg ccaccagtga
      181 atatcctcaa ggtatacagc gcacaacaaa aagaaaaaag gaaacgcaga agcatttcct
      241 ttcagtgatt taatgaaaac aacacgattt gacttagagc ctttttattt gaattcattt
      301 tcgctttatc caacttcatt tgatcatttg attactcggt taaatacttt tgacatgagt
      361 atcaatgaaa cattcattaa ttatggttgc ttatttctta ccgcttttct ggccaatgct
      421 aatccgccga ttattccggc attatcacct aattcgggcg taataatgta gctatcaatt
      481 ggtggtattt caacatatcc atttataagc tcaacaaatt tatcctgaac ttttttcttc
      541 atgtgttctt gtttcattac accaccgcca aaaataattc tatctggcga aaacatcagt
      601 gttgtattgt atgcacattg ggcaaggtaa aaggcttcaa tctgccaaaa cgaatcgtca
      661 gccaataaat tctggccttt tacacccaac cgtttttcaa tcgctggtcc tgctgccatc
      721 ccctctaaac aatcttggtg gaagggacag tttcctgaaa aattgtcctt tggatgtcgc
      781 ttgatcaaca tatgccccat ttccggatga ctgaaccctt ccacgaaagt cccattgaac
      841 aaggcaccac ctccgatacc tgttcccaca gtgaagtatg caacacctgc taaccctttg
      901 ccagaaccag caacatattc accatagcaa gccgcattga catctgttgt ccattcaatc
      961 ggaatattga aatattgctt tagcgttcct acaaaattga aattttgcca agcaagtttt
     1021 ggtgttgatg tgatatgacc gaaactcgtt gatttgcatt gaatatcaat tggaccaaag
     1081 cagccgaccc cgatgcctgc caattgttcc ttaaaaggtt taaaaaaatc aatgacctgc
     1141 ttcattgttt caactggagt tgttgtggga aaactgactc tttccaaaat cgttaattcc
     1201 tcagtaccga ctgcacagac aaatttagtc cctccagctt caattgcccc atatatcatc
     1261 ataggttcct cccttcgaat atgtgaaaaa cacttcatta acgcggcaag cgttctcgat
     1321 cagtgaaatg acgtttagta tttcctgaag cttctgttga ataccagtaa gcgacactag
     1381 caacatcgtc ttgccgctca aataagcgaa catcatcgtt gccgatttgt tgtaaagtga
     1441 ccgataaatc ttcggtaaaa cagatggggt cttgaagatg ccaacgataa agaccatgtc
     1501 gtggcattgc gtcgtcacca aaggcatgaa cggcattcaa ttttcccgtt tcaaaacgat
     1561 ctcttgtggc atctctattt gattggtaag gataccctaa aaaggctgtt gaatagtttt
     1621 tcgcatgtgg acgtccgaac tcatcgtact catgaaaggc ccacgccccg ccaaaataat
     1681 cttctgaacc tgttgaggtt acggtcggat actcactatc cccatcaatg tagaatttaa
     1741 actcgccttc gccccaccaa taacgttcta ggcaggtaag cgcaaggtaa gtacccacgt
     1801 aatagccttg accctttaat cgatcaatta acttataatc ctcctgtaat ttggtaatgc
     1861 gttcccgatg ccaatttgcg tgaaaatata ggctattttc ttccaattcg tccaccaaag
     1921 cgtaatttat cgtataaaaa aatgaggaca tatccttcgg atgtaaattc tcgatcgtaa
     1981 ttttagcgga tgtttggaaa ggcatttcga aataggaatt aaagccccct gtaggattaa
     2041 ccactatcgg caacgagttc acctcgcaac gctctccaaa cccattacaa aagaaatccc
     2101 ctaaaggact ttcaacagca gggctatccg acccatccca ataaattctt aagattaaat
     2161 cacgtaaaac aaaactgcca aattctgttc gatcacgaat cgtcatccac atatgacgaa
     2221 ttattcccgt tccagtaata tccgcaatca cagtttcttc accagaagtc agcggaattg
     2281 cgccacgacc tttgcggctt ggtcctagct cgcttggcaa catcgcagct ttacctttct
     2341 cgcctgttgg attttcggga gtgattgctc gactctggat ttttttaaat tgggtaatat
     2401 tttttagtat actcatcttt ttctcctttg aatgtgaatc gtttttccct tttctacgtg
     2461 aagaattttt ttcgtatcac ttattttcaa tggcttttct gacaaatttg aaatactcaa
     2521 ctcggattgc gtacattcaa tcgaaatgaa ggcttgaccg aacttcaaat tcttccaaga
     2581 aaacgttaaa ttatcaacag gatcaatttg caatactcct tttttgaatt tcaatccgaa
     2641 atattgactg atcattaggc tgacaaaaag acccgatgcc cagccgcatt tgccgcagcc
     2701 aaagtctcgc acgacttctc cttgttgagc ctctaagcga tacggccacc accaccacga
     2761 accgtctaaa tctgccaaac aaattagttc ttctaatcgg gttacgaaag cttgagggtt
     2821 gtgcagctcg ctcattaaaa cagtgataaa gccaggaaaa gtggcgccgg attctaatcc
     2881 ccaagtgatg cctttgattt cctcgctata ggttggattg aaagaactag cagaaaaggc
     2941 catggtgttc tgatataaat gatctttatt atctaaaaat tgataaaaag gcatcaacgt
     3001 agtgtctgat tcttctccat catgcatcaa taaattaatt tttccatcca caatcacatc
     3061 acttaaaaag attaaccctt gctcaagaat cggtttttga tagtgcttca ctgaataggt
     3121 tgttttctcc tcatcaccga ttccctccaa aaattgctca ccaaaaggac catcgatagt
     3181 catttgttta caaattgatt cctttagttt acaggcaatg tttttgtatc gcttgctaag
     3241 actcagttgt tctagggctt caaaaatttc tgcaagtcca atacaggcct tccaaagaca
     3301 aagattgctg ccggtatgat actttccaag tgcaaaggcg tctgataacc aaacagatgg
     3361 aaaaagcatt ggttcccctg accgacgttg agctaatatg tcctcaataa ttcttgaagc
     3421 attcagtaga acctgcggac accgctcaaa gaattttgta tccccagtat gttcaaaata
     3481 aagactagcc agtaacgcac tggataacga attactcaga gaatgattga tacctcctgt
     3541 aaattttgaa ccaggaaact taacgccata ttgatcaaac catagaaccg ttttttgaca
     3601 taactccggt tcaaacatgg tgaatggcaa tgatgcgtaa tacatatcct tattccaaat
     3661 tcgattagta gcaggatagc tgccccaatt gctgccgacg acctgtccct gtcgattcat
     3721 accgaaacta gaaaagcttt gataaagtgc acgacgatag agatggacaa ctctatcgtt
     3781 tggcattgac agctgcccaa atagctgagc aaaatagttc aatgtttcct tcagccattt
     3841 ttcgatatca gattcctgaa aagtcattac ttcttcaaaa gcatttgggt ccacaaaagc
     3901 aatggcgaag cttgccactt cctgttgtgc gatcgtgtcc ttatttcctt tttgagaaat
     3961 caacacattt tgctgatccg aatacttctc ttgatacgaa ggcacatctt tcactgaaac
     4021 ttgaaggctt tcgtgtgttt cattcacaat gtaaacctgt tgaacaagca tacttaaacg
     4081 cttattagcc agtatgggag caaaggatac tacaatgacc ttaaacgtcg aataatgatg
     4141 aatatatcta ggcaaacagt cttgaatcac atcgacttca atacgatgat ccgattctga
     4201 caaatggtag acaatcccgt ttacttcaac ctttaaggat aacgaattgg tttgagagag
     4261 ctgtttattg acccaaacac ctggtttttc ctcagttagt aaattttctc gataaaaggt
     4321 caacgtttga atcgtcccgc aaacatcaag gtttgctaat gtcttttgat tacttacatc
     4381 gaaggtcaat ccctttacat caggaatatt ccgatagtag ctgtattcgt ttgaagtagt
     4441 cacatttccg tcgtttaact cagcattaaa aaatgggtgt ttgggtgaat aaattacttc
     4501 attaatagtc atcttttccc tccttgctaa aaaaactgca gcctagtagc tgcagcttcc
     4561 tactatgcga ttccaagtaa tgacaataaa attcccaaga cgaccattcc aaaaataatt
     4621 ttgatgacgc cgactttttt atctagtaat ttgaaacagc tcaatgttac caataacggt
     4681 acaattcctt taaaaatact atcgaggatt tcttggacat tcattagttc ttcgcccttc
     4741 attgaaaagc tcaaaacggt attgaatttg accatactga tcgtcattgc acctaccatg
     4801 atcagaccca agatactagc agcttttgtc agaatttcaa ttaacccgtt cgcatacagc
     4861 ttttcaatat atttagagcc taatgaatag cctaaaacgc cgccatagta tcgcatcaag
     4921 taattaggta tattgaacat aactaaaaag ataattggtg ccaaaatatt tccggcattg
     4981 gctaagttga tcgctagtcc cgcggtaacg acacgccaaa ctccccagaa caatgaatcg
     5041 ccaattccag ccaatggtcc catcaaactc gttttgatcg cgttgatact ttccacgtca
     5101 aaaccagctt tttcagaatt ttccttctcc atcgatgccg caatccccat gacaaaggac
     5161 gcgcaaggaa tggtcgtcga aaaatactgt gtatgacgtt ccagcgccgc agctcgatct
     5221 tcttcacttt tgtagaaatg attaatgaag ggcatcatag aataggcgaa accaccgccg
     5281 cccatcgtca ccggattcac cgaagcatac atagtcatcg agcgccaaaa catcttccga
     5341 accaattttt tctcatcttt agaaattcca tcagctaatt tcattgaaag aaatcctcct
     5401 cttcttggtc aagcgttgca tttacagcag catcgctttg aactgcatga acagatatat
     5461 tttttagttg tagatctctt tgggctataa caattgcaat aatcgttcca actgcggcaa
     5521 ttgcaatcaa tggtaactcg agatacgcca taacaacggc cccaaagaaa aaccacacag
     5581 caatattttt actccaaagc atccgtaaca gcatcgccat cccaactgcc ggcagcagat
     5641 ttccagcgac tgttaatccg ttcatgatga cctctggaat attatccaag acgcttttga
     5701 caacatctgc tccaaataaa acggcaaaga atggaataag agcataaagc gcatagtgga
     5761 caaaccacat aaagaagtgc agattgatga ttcctttttg atcaccagat tttgcaaatt
     5821 tgaaaaattt agcagccatc ggtccacata caaaagaaaa aatcatattc attaactgta
     5881 atcctaatac tgctagtgga aagacaatgg gaataatcgc atccacgttt cctcccattt
     5941 tgattgcgaa cgctgtagcc agcccagcag ccaaagaagg ctccgccgta ctagagattc
     6001 cgatattgac gacacccata aagatggctt ctagtgacgc cccgacaatc aaccccgtct
     6061 gcaaatcccc catcaacgct cctgctaacg gagcaacaac aatcggacga ctcagcatgg
     6121 acatcccgaa catttcatgt ccagcaactc caataaaaac ggccaaagcg actacaaatg
     6181 catcatacat aatttttctc cttccttttt ctaaaaatca ctcaaaatag agatagctga
     6241 acgtttttcc atcgccggtg taggctgaaa aacggtatct ggataaagtt caactaattc
     6301 ttttaagtct tgaatttctg acgtcgttaa catcacttcc tttgataagg ttttcttatc
     6361 ttccgatggc ggtgtcattt tcccagcatt ccccacgttc attaattcga tttccgttgg
     6421 gaaagctttc gcaactttta ccgcatcttt gacattaggg actaaaacca tcaagcgcat
     6481 gtccttcgcc cgcggatctt ttaacaaatg aatcgcgtca tcaactgttt taataatgac
     6541 tttgacattc gatggtgcag ccatcttcaa tgtcatcttt tgaatatcgt tggtagccgc
     6601 ctcatcgctt gctactacta acccggttaa atttagactc tttgtccaag tcatcgctac
     6661 ttgtccatga attaatcgtt catcaattcg cactgcttta atcatttttc ttcctcctaa
     6721 aaaaaatctt cctcttcatt tactacatgt acctcatcaa gaatggtcat ctttaattcc
     6781 tgttggcatt tatttaccag ttcctgaata tcctcctttg ttaattcctt ggttgccaat
     6841 actatttcga gcagaattgg cagattaaag ccagacaaaa tagttacagc aatctctttc
     6901 tccaaaaagg ttttggtaat cttctgattg acacttccgc caaataaatc ggtaaaaaca
     6961 aaaatctgat cctgctcgtt cacctggttt acaaatacat tcaactcctg ttcaaaatca
     7021 cactcatcca cataagcatt aatcaccgta atctcttgaa ccttccccgt cagtaattga
     7081 atactgctaa gaaagcctga agctaagtga ccgtgagtcg ctattagaat tttcttcata
     7141 tggttaattc ctttctttct gttcactttt atataagcaa ttcctgtgcc aacttttaaa
     7201 aacattggtt taatcacgtt aattcacatt aaaaaaagtg tctaacctaa aaaaaacaga
     7261 ttagacactt ttttagacac tttatttttg aatatacggt cctaataatt catagagcag
     7321 cgaaagctca taagcatt
//
LOCUS       DAFFSV010000127         7216 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFFSV010000127 DAFFSV010000000
VERSION     DAFFSV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.127"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            234..503
                     /gene="rpsN"
                     /locus_tag="LR110_002447"
     CDS             234..503
                     /gene="rpsN"
                     /locus_tag="LR110_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL4823386.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            518..697
                     /gene="rpmF"
                     /locus_tag="LR110_002448"
     CDS             518..697
                     /gene="rpmF"
                     /locus_tag="LR110_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL4823387.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            729..1106
                     /locus_tag="LR110_002449"
     CDS             729..1106
                     /locus_tag="LR110_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823388.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1127..1276
                     /gene="rpmG"
                     /locus_tag="LR110_002450"
     CDS             1127..1276
                     /gene="rpmG"
                     /locus_tag="LR110_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL4823389.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1300..2283
                     /locus_tag="LR110_002451"
     CDS             1300..2283
                     /locus_tag="LR110_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL4823390.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2313..2435
                     /locus_tag="LR110_002452"
     CDS             2313..2435
                     /locus_tag="LR110_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL4823391.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2453..3976
                     /locus_tag="LR110_002453"
     CDS             2453..3976
                     /locus_tag="LR110_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL4823392.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4000..4239
                     /locus_tag="LR110_002454"
     CDS             4000..4239
                     /locus_tag="LR110_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823393.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4432..5067)
                     /locus_tag="LR110_002455"
     CDS             complement(4432..5067)
                     /locus_tag="LR110_002455"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL4823394.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5088..5306)
                     /locus_tag="LR110_002456"
     CDS             complement(5088..5306)
                     /locus_tag="LR110_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823395.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5432..6636
                     /locus_tag="LR110_002457"
                     /pseudo
     CDS             5432..6636
                     /locus_tag="LR110_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            6631..7038
                     /locus_tag="LR110_002458"
     CDS             6631..7038
                     /locus_tag="LR110_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL4823396.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
BASE COUNT         2548 a         1078 c         1415 g         2175 t
ORIGIN      
        1 ctaataaaag tgaattctta tttttcagag cttaaactgc atattctttt aaatgatttg
       61 attatctcta tgtaccgata aattcgtttt caaatagtta tctattcata tgagttttat
      121 ttcatgttat ttattgttga caagagcaac tcttgttggt aaagtatcag tgtcaaaacg
      181 taacgattac gatttgagat gtctaaatca aaataaaagg agtataaagg tatatggcaa
      241 aaaagtcgaa aattgctaaa gcaaagaaac aaatggcgat gattgaaaag tacgcagata
      301 aacgtcagga attaaaagcc gcaggagatc gcaccgctct cgctaaattg ccaagagatt
      361 caaatccgaa tcgtctgagg cttcgtgatc agacggatgg tcgtccaaga gggtatatgc
      421 gaaagttcgg tatgtcccgt attaagttta gagaacttgc ccatcaaggg ttgattcctg
      481 gagtaaaaaa agcaagttgg taaaaggagg aaatatcatg gcagtaccag caagaaaaac
      541 atctaaggcg aagaaacggt taagaagaac tcatcaaact gtagtaaaac cagaaatttc
      601 atttgacgaa gcgaatggtg attatagacg tagccatcat gtatctttaa aagggtacta
      661 taatggaaag aaagtaatta aaggagcttc aaaatagttt aatatataac ttttaggagg
      721 gaatcaaaat ggaaattatt tacccacctt tagttgaaga aggattaaag tattatcttg
      781 aaacgaccca gcaatcgcta gataaatgca ctttttatcg ttcgatggta gagagaggaa
      841 ttattactga aacgggttta ccgacccaac aggcaattga aaatggatta gtcaaagatt
      901 actatgagga ccaaggctta tcatttgatg attttttaag aatctatcca atttttgaag
      961 agtatgacga ggagttattt caatgtattg atggttattg ggagatacct gttgatatga
     1021 aagaaaattt agtttcacaa ttggaatctg gcgagttaac ttttgaagat gcacaacaaa
     1081 ttcaagcata tcttgaagat cgataagtat tgaaggagga agatttatgc gtcaaaatat
     1141 tattttggaa tgtgtcgaaa ctggagaacg tctgtatctc accagcaaaa ataaacggaa
     1201 taaccctgaa cggttagaat taaaaaagta ttcgccaaaa ttgcgcagac gggcaatttt
     1261 caaagaagtg aaatagctaa gtggtaaaaa ggtgacaaaa tgggcatacc agttacaatc
     1321 gtttctgggt ttttaggggc tggaaaaaca acgctaatca atcaagcctt gcaagtctca
     1381 ccctacccca gggaagaaat tattataatt gaaaatgagt ttggtgaagt tgggattgat
     1441 cacaaattat tagttcatag cgaggaacgg gtgttacaac taaataatgg atgtatgtgt
     1501 tgcagtcttc gcagtgattt attagcaata ttgatttctt tactggaaac aatagaagaa
     1561 catcaacaac caatttcaca aatcatcatt gaaactacag gtatagcaga tccccaacca
     1621 atcattcaaa cacttttaac agcgcctcaa ctaagaggaa gagtatatat cgatagttta
     1681 ttgactgtta ttgatagtga cagttttgaa cttattgaaa gagaaccaca agcccttaag
     1741 caacttgtca tagcagatcg catttttgtg tctagaaaac aaaacttgaa tttatctgca
     1801 ctaaaaaaat tacagagaga aatcaaaagt attaaccctt tatccgatat tcgaatgttc
     1861 acacatagtg aaacgattca aaaaaaggat ttttataatt tggaaaaatt caatcatccc
     1921 ttagtttttg aaagtgattc atcagaaaat cactataatg gcaaacacta tcatcaacat
     1981 catcaagaac atgagtttaa atcaattctc ttaacttcag atcaagacat aaaaaaagat
     2041 tttttattcc aattaattga ccggctgatt ttaaccaacc aagggagcct gtatcgtttt
     2101 aaaggattga ttgctttgca agatcaagat tttatagttg ccatacaagg ggtaaacgaa
     2161 caggttaatt ttcaatacac cactcgacaa aaagaccaaa ccactattat tctaattgga
     2221 aaaatgttgg atgaagaaag gatcaaacag agttttgata aattactggc ggaaagcaaa
     2281 taatgataag tattgattag gggtaatttc taatggagaa aacggatata tctagtgcat
     2341 atcgacgact taaaagcccg aatatcaaaa ctagaaaaag ggcattaaaa attatcaaag
     2401 aacataaaca aaataaaatg aagaaattag cataattaag gaaggaaaaa gaatgaagaa
     2461 aatcagtgta ggacttattg ggatagcagc tctaggatta ttgggagctt gctccagcac
     2521 taatgatgca aaagtatcga atgataaaga tggaaaatta gaaattgtaa caacattcta
     2581 tccaatgtat gatttcacca aaaacatcgt cggcgatgaa gcgaacgtgg atttaatggt
     2641 tccagcggga tcagaacctc atgactatga gccctcagct aaggatatgg caaaagctca
     2701 tgatgctgat gtttttgtat atcataacga aaacatggaa tcatgggtac caaaagctaa
     2761 agaaagttgg aagaaagctg ggcctaatgt agtagaagga acaaaagaca tgattttatt
     2821 gccaggtagt gaagaagaag atcatgatca cggagaagaa gatcaccatc atgaactaga
     2881 tcctcatacg tgggtttcac ccaaaatggc aattaaggaa gtttcaaata tcaaggatca
     2941 gttagttaaa ttgtatccga aaaaagcaaa agtatttgag acaaatgctg aaaagtattt
     3001 gactaaactg aaacggttgg atgctgacta taccactagt cttaaagaag cgaaacaaaa
     3061 aagttttgtg acacaacatg cagcctttgg atatttagct ttggattatg gattgataca
     3121 agttcctatt gctggactaa gtcctgaaga agagccatct tctggtcgat tagctgaatt
     3181 aaaagagtat gttaaaaaaa ataagatcaa ttatatttac tttgaaaaga atgctaatga
     3241 taaaatcgca aaaactttgg ccaatgaagc tgggataaaa ttggaagtat tgaatcctct
     3301 tgaaagtctt acaaaagaac aaatggataa tggtgaagat tatgtttcag taatggaaga
     3361 caatctaaaa gcgttagaga aaacaactat ggttgctggt gaagaggtag ttccagaaaa
     3421 agaagcgaag gatgaaaaga cagttgctaa tgggtacttt aaagatgccg atgtgaagga
     3481 tcgcgaattg agtgattata caggcgaatg gcaatctgtt tatccgttgt taaaagatgg
     3541 aattttggat gaagttttcg attacaaagc aaaactcaat aaagacatga cagctgccga
     3601 gtacaaagat tattatacta ctggctataa gacagatatt gatactatca acattaaaga
     3661 caacaccatt gattttgtag tcaacggtga acatcatcaa tacacataca aatatgttgg
     3721 atacaaaatt ttgaattatg aaaaaggaaa ccgtggtgtt cgttttaatt ttgaaactga
     3781 tgatgcaggt gctggtcgtt ttaaatatat tcaatttagc gatcatggaa ttgcaccaag
     3841 taaggcagag catttccata ttttctttgg tggagaaagt caagaaaaac tttataatga
     3901 aatgcataat tggcctactt tctatccagc ttcattatct gagcatgaga ttgctcaaga
     3961 aatgatggcc cattaaaatc caatctttgg aggaaataca tggtaagaaa agtattattg
     4021 tctatggatt ccaagaatat tgatgcttat cggcgttatc aatttgcaaa gcaaatgaga
     4081 ttaaatggga gtacattagc tgatattgct gcaactttac atgttagtat tgacacagtt
     4141 catagagtat taaaacggga tcctgattgt tgttttatca aggtcaatac tggaagaagt
     4201 agacgtttag caaaacggca taatagcttg gccaattaat tgataatacc agagtagatc
     4261 atttttccct tatttggatt tgatctgctt tttattattt cttgaattgt caaatcaagc
     4321 gcaacacttt atcaaagaag acttcataag gtgttctcca atctaaacac tttctaggtc
     4381 tgttattcag tttttcaatc atctgattta tcgttgagtt atctaccgta gctatctctt
     4441 tcgattttgg caagtattct cgaatgagtc tattcgtatt ttaatttgtt cattgttgcc
     4501 aagggcatga ggatctggaa aataaaaggg catattattc aatacttctg taactgcgaa
     4561 atgattggag aattcttttc ctctatctgg agtgatcgtt ttgaccgcct catttggaat
     4621 actgcttaaa agttcaatca ttttctcgct cacataagcg gctttctttc tggggacctt
     4681 ctttcctgct aaaaggaagc atgattttct gtctgtcaaa gtcactaaac aagtaccact
     4741 agttttgcca gctacaatat cggctttcca atgaccaaaa tcacttctat catctgcgga
     4801 ttttggatgg ccatggatcg aatgagtaag gcaaatcttc ccacgagact cgacatgatt
     4861 ttttgtatgt cttgtttttc ctcgatggtg caatgaacgg atcaggcctt tattgccgtg
     4921 tgagaggggt tctgtatcga aaagatcatt gcaaattgca cggtagatcg tgataaaact
     4981 aattgaaaag tccatatgtt catgcttcaa acgattcgag attttctctg gcgaccactg
     5041 aaaatcgaca aataaatgac gaacaattga ccaaactcac ggatcattta gaatgtgata
     5101 acgtccgcat ttttttcttc gataagcata atttttttcc gctttggaag gtgattagtt
     5161 tttttcttgt ttcttctcag ttcatgtaaa atagtaaacg ggctatgctg aatgtcgttg
     5221 ccaattatac taatagaaaa tccttcttcg tgaagcagaa agattctttc tcgtttgagt
     5281 atagtaagat gacaatactg gctcataatt attttccttc taggagtgtt ctcgacaaac
     5341 ttatactagt tggatatcga ttatgactca tttttatgtg ttgcacttaa agtgtaaatt
     5401 caaggtatag aaagaggttc cctatgaaaa aatacctatt acaatcttaa ctggctatct
     5461 tggttcagga aaaacgatac ttctaaatca tattctgtac ggtgatcacg gaaaggaaat
     5521 tgctgtcatt gtaaatgaca taggggaagt aaatgttgat ggggtattag tcgacaagaa
     5581 aggttttaaa agaactggag aaaaacttgt ttcgatgccg aatggatgca tttgttgtac
     5641 attacgcgaa gatttgattg ttgaactaga tcaattagtg caattttctg acttgaatca
     5701 aattgttatt gaagcttcag gaatcagcaa accaattcca attattcaag caattatatt
     5761 agctaaaaac cctttagaca ttgatttgac ccaacgatta gagattggtg ccattattat
     5821 agtagtggat gtaaaacgtt tttgggataa tttcgaaacg ggtgaaaaga ttcaggaaag
     5881 aaaagctgaa ggtaatgtag ttgaagaaga acaggatatt cgtggcttgt taattgatca
     5941 attcgagtgt tgtaacatat tacttttaaa taatagtgac ttagtgtcaa ccaagacgat
     6001 ttacaaaatt aacggtctaa ttgctaagtt acagtccaat gccaaaataa ttaggacaat
     6061 taaaggtgaa gtagacttat cgaaaattct aaatacaaat ctttttaata tagatgagac
     6121 ttcctattca atgggctggt tcaaagagtt agagcttggc tatcaaaatc atattcctga
     6181 aacagaagaa tatggtatct caagttttat ctatcgaaat cggttactcc ttgacgcaca
     6241 aaaatttgcc catcttacgg ataaaaattt tcctggcaat attacaagag caaaagggtt
     6301 tctttggttt gcccaacata gtgatttttc gacgatcttt gaaacatcgg gtagccataa
     6361 aagtattgaa tcattaaatt attgggtagc tagtttacct aaaattgagt gtcaaatgat
     6421 tatacaaaaa aacccgaatt gaaggaagcc tggcatccgt tgtttggtga acgcattaat
     6481 aaactagttt ttattggtat gaagatggat caacaatgta ttaaggaaca acttgagggt
     6541 tgtttaactg attctcttaa tgcagataat ttccgaaaaa tttcaccatt tccatggaaa
     6601 aattctaata tgtttagcca gatatcgtta atgaaatttt taagaaaagc tggtgtcaag
     6661 ataggcgatg tcataattat tggcattttg attattccct cttttattcc cttatttgta
     6721 ttctctatag aaaaggagaa tgaaacaact gaagatgcta tcaaatatgc catcgttcaa
     6781 attgatggca aggaaatgga tcgcttcgat ttggataaga tcaatcataa actcgtgaat
     6841 tatcatcctg cgaagggaca atataatatt gttgagatta aagatggaag aattcgagtt
     6901 aaagaagata atagtcctga tcaaatcgcg gttagaactg gatggatctc aaaacccggt
     6961 caaacgagta tttgtttacc ccataagtta gttattagta ttgagaaaaa agaaagcaaa
     7021 gattactaca tttactaatc tggaaataga tgaggaggtt tttaataaga aaaagatatt
     7081 attattaata atagcagggg ctttattaag tgtgttaagc gcttgtggac aagaaaaaga
     7141 agcggtgaca acaggcacaa gtatctttaa cgggacctgc aatggtttag ttaacagtcg
     7201 gttagattac ttattt
//
LOCUS       DAFFSV010000128         7072 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFFSV010000128 DAFFSV010000000
VERSION     DAFFSV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.128"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            78..263
                     /locus_tag="LR110_002459"
     CDS             78..263
                     /locus_tag="LR110_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823397.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            768..962
                     /locus_tag="LR110_002460"
     CDS             768..962
                     /locus_tag="LR110_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823398.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1084..1402
                     /locus_tag="LR110_002461"
                     /pseudo
     CDS             1084..1402
                     /locus_tag="LR110_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1646..2476)
                     /locus_tag="LR110_002462"
     CDS             complement(1646..2476)
                     /locus_tag="LR110_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBL4823399.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2684..4018
                     /locus_tag="LR110_002463"
     CDS             2684..4018
                     /locus_tag="LR110_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL4823400.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4059..4970
                     /locus_tag="LR110_002464"
     CDS             4059..4970
                     /locus_tag="LR110_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4823401.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4957..5820
                     /locus_tag="LR110_002465"
     CDS             4957..5820
                     /locus_tag="LR110_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4823402.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            5817..6434
                     /locus_tag="LR110_002466"
     CDS             5817..6434
                     /locus_tag="LR110_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL4823403.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            6436..6954
                     /locus_tag="LR110_002467"
     CDS             6436..6954
                     /locus_tag="LR110_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL4823404.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2124 a         1212 c         1408 g         2328 t
ORIGIN      
        1 attttgagtc actttttgtc aatattatta tttacaatac cttctataat aaaagttatt
       61 taaggaagtg tctatgaatg agtaaaaatt ctaaaggatt cctaaccatc ttattagctt
      121 ttatagggta tatgttggtt gggctgctaa aatcatacag taatgagttg ctgaattttt
      181 caacatttat taatgataca ttagttccaa gtttgttttt cattgtattt tttgctgttg
      241 gttattttat aataaagata tgatgactaa tttagctcaa ctattccagc gtcagctgaa
      301 gatattgcaa ttcccgattc cttaatcatt tatctggatg agacagaaag tgaagttgca
      361 acagatgtgc atgtagttga ggataaaaat gatagtttaa aaattaatag ttcagatact
      421 ttactaagag taagtcctat cttttcagct gttatttaga tgcaagtagc aactaagtta
      481 ggtagtactc ctgcaatgga taggtatttg tgtgttcgat atataagtgg tcgaagtatc
      541 aaggttatgt atactggatg ggagatagaa gagttaaaaa ttggaggtca ccgccacaaa
      601 tggaatttag atttagaggt accttagatt tggtatagtt attaaacaca agatggcctg
      661 gtttgtaggg aaagtcgaag agtatttttc cacttctgcg cccctttaat ttttctagta
      721 atgccgtgaa tttgtgatta attggaaata tgaagaaagg agagtctatg aaaacaacac
      781 tagttctatt ctataaaaag catccttatt ttacacttct gataaatata ttgttggcta
      841 gtgttatcgg gatttctgtt gaatatctaa taaataaaga tttcataggt tcttgttttt
      901 atacggcttt atttcttgga cttcttgaag cattttccat ttataaaaaa tcaaaaaaat
      961 aaaatagatt gccgtaagag atctttttca tttcattttt ttctgaaaac ccgttgtttc
     1021 tgtcagtgaa gcagcgggtt tttagattta ttgtctttag acctaattga gcgcgccgag
     1081 cgattgaaat acaaaaccac ttgctatgcg ggtggagcaa taaagggaaa tagtttagcc
     1141 cgtacaaagt agatgtgtaa ataccacatt gtattcacac caaagtatag aagaaaaata
     1201 atttataatc aatatcgagc caatattcag gagatcatca agagattgtg caaatctaaa
     1261 atataaattt gggtatcgac acttttgggt ggaaggatac tatgtgacaa aagtaggaca
     1321 aaatgaagcg accatcaaaa agtacattca agaacaagaa aaacatgata gtgcgttaga
     1381 taagctgagt gtacgagagt aagacgatcc ttttaagggc taaggagtag tacataaacc
     1441 ttttaagagg tgggcaaaac tggcaaaggc aatatggctt gaacataaaa tatgaaagcc
     1501 agcgtcttta ggcgctggcc ggtgatttga gcttatagct ctagagaaaa ccaccctttt
     1561 gaagggtggt tatgattaat gactttagtc agttttgctc acaaagcgtg tggaacaaaa
     1621 aacgggtgtt tctacgggta gatatttatt tcttgaatgg aggtgtccca tacatttttg
     1681 gtgttactgg ttctaatttt ggtgcagcgc cttcgatctt aggttctttc aaccattgat
     1741 attcgccttc gccatccagt gctttgccac tagcccaaga accttcacta ctttggtctc
     1801 cctctgagaa gttatagaag tcataagcaa tgtcttttcg ttctttgtca cgcgggaaag
     1861 tacttgggac taagattgaa ccttcttttt cttctaattc tttcactgca cattcccata
     1921 gattttgatg atagctatct cttgcaagga gaacagacag catatctttt acgccacgat
     1981 catcgatcat ttcgtaaata cgtgctactt gaagtcttcc ttgtgattcg gcatttaaat
     2041 tagctctaaa gtctgctaac aagttgccac tcgcaatgat ataggcaccg ctccatggat
     2101 tgcccacgct gtcagcagga cgaggtccta agccagctac gatcgcgtgc tgtggattca
     2161 ttccagagat aatggcaccg attgttggat ctttttgcat cgctgcttct tgatcttcaa
     2221 caggcgcacc ctctaataaa cgagcaacca ttgtcgccaa catttccacg tggccgattt
     2281 cttctgttgc aatgtccatc agcatatctt tatatttttc ttctcctcgg cagctccagc
     2341 cttggaacag gtactgcata gctacactca tctcgccata ttggccgccg atcagctcct
     2401 gcagatattt cgcgattaat ggattcggac gatctggttt tgcttcaaac tgcaattctt
     2461 tctggtgtct aaacatcaaa attcctccta atctattttt aatacattct cattctgcta
     2521 cgaaagatta aaaaattcaa agaaagcgct cgcgaattat ataaagtatt ggtatcatta
     2581 ataatttttt tagaatcata ccttataaat tgttattttc agaacaaaat gatatcgtta
     2641 tattaaaacc ggcatcggtt acattaaaat ttggaggtaa aatatgaaaa aattattgtt
     2701 tacagggttg ctggcaagtt cagcattgtt tttattagga ggatgcggat caagtaatgc
     2761 aagcgatgcc agttcaagtg cagcaaattc ttctgaaaaa acaagtgatg gaagaaaaaa
     2821 tattgatttc tggtcctttt ggggatccgg tgcacgaaaa gaagctatcg aagaaatcat
     2881 tgaggatttc aatcaatcac aaaacgagat ccatgtaaat tataagtatc agccatgggg
     2941 tgacatctgg actaaatctt tatctgcaat aacagctgga aatgcacccg atgttattgt
     3001 acaggacata aattccgttg cccaaagagc agaagcacag caagcaacta atctctccaa
     3061 atatgtagaa aaagacactt ctaaggattt ttatccgcag ctttgggata cagtagaata
     3121 tgaaggtgat ccttatgcga tcccattcaa tacagataca caagtcattt tttataataa
     3181 aaagatattt aaagaagctg gaatcagtga acaagatcta ccgacaactt gggatgaatt
     3241 agaagaaaca gcacgtaaac tagataaaaa agaaggaaaa gaatttacac gcatcggttt
     3301 ttatccatta tggaatttag gggctgatgt atgggcgcta aacgcagata acgggactag
     3361 ttggtttgac aaagatgaaa aagtcaagat tgatactaaa aataaagtgg aagccttgga
     3421 gtggatccaa gactggcaag attattatgg gaaaaatacg atcaataaat tagaagccga
     3481 atttgggtct ggtgtatctg atccatttat ttccggatta gtagccatgc gtgcccaaaa
     3541 tatcaattat tactctagtt tgatggaaaa tgcaccggat gattttgatt ttggcgtgat
     3601 gcagctccca gaaaaagaag aaggttcagg ccattggtcg tggggtggcg gattcgtctt
     3661 ggaagtacca tacggagcaa aagatcctga ggcctcttat gaattcatca aatatctttc
     3721 ttctccagaa gtacaagaaa aattcggcga aaaaagtttt gatatcatgg cgagcaagac
     3781 agccaatgag aatttagtga ataatgacaa cctaacggat aaaggaaaaa tgatctatca
     3841 aatggcggac gaaaacttta aaaatacggt gatcacacca gttcctttag atgctccgga
     3901 ttacacgaca ttagtgaatg aacagattga ccagattctt ttaggaacaa aatcaccaaa
     3961 agaaggattg gcagatgcac aaaaagcagt agaaaactta gtcaagcaaa atcaataatc
     4021 catttttgac tgtctaggta cagaggaggc aagaaaattt gaagcgttta tctatccgaa
     4081 agaaaaaaga agcaagatgg gcctatttat ttatctcgcc ttttattatc gggttcttgg
     4141 tatttatgct agggcctatg cttttttcag ttattggtag tttcaccgat tataatttga
     4201 catccaagat gaatttcatt ggtctagcaa atttcaaaag aatgttcgtt cacgatgatt
     4261 tattttggaa atcgttatac aacaccgttt atttcgtcat cttcaatgta ccgctgacga
     4321 cgattttcag tgtgttttta gccacagtac tcaatcaaaa aatcaaagga atcagtgtat
     4381 tccgtacagc tttctattta ccggcagtct tatcaggagt agcagtctac atcttatgga
     4441 tgcagctgct ttctccatct gcaggattga tcaacacatt tctgggctgg ttccatatcc
     4501 ctggaccagc ttggctgttt gatccacaat ggacgaaacc tgcgttgatt ttgatgaaaa
     4561 tatggagttc tggaggagca atgctacttt acttagctac attacaaaat gttcctagat
     4621 ctttatatga atcagcagaa atcgatggag caggcgtttg gggaaagttc aaaagtatta
     4681 ctttgccatt aatcactcct attatttttt acgatgtgat cacctctacg atcggttctt
     4741 tccaaatttt tcaagaagct tatgtcatga cgaaaaatgg aacaggcgga cctgcaaatt
     4801 ctttgctttt ttataatctg catatgtgga acaaggcgtt cgtagctttt gatatgggtt
     4861 atgcgatggc tatgtcaatg atcctgtttc tgatcgtgtt ggttctgacg tttgtcaatt
     4921 tgaagcttgc acctaagtgg gtctattatt ctggaggtga cggcaaatga tggattattt
     4981 caaaaagacc agtgtgaatc ggcaaacaaa aaaatactaa gttatgtcct catgacagta
     5041 ataggaatca ttttgatcat tccattgctt tggatggtgt ttacttcatt gaaaccaatg
     5101 gaagaaatcg ttcgttatcc tccgaccttt ttcccagaaa aaatcgtatg ggaaaactat
     5161 ctggatacga ttgccgcctt tcctttttgg cgctatgcaa gaaatacatt attcatcact
     5221 gtgctagtag tgatcggtaa tgtcctttca aattctttta ttgcttatgg atttgcgaag
     5281 cttgattttc ctgggaaaaa gctgatgttt gctttggttc tttctacgat gatgatcccg
     5341 gggtttgtaa cgatgatccc tcaatatgta ttattctcca agatcggctg ggtaggtact
     5401 tatttgcccc tgatcgttcc atcgttcttc ggcaatgcgt tcaatatatt tctgatgcgt
     5461 caattttact tatcgatcaa taatgaattg atcgaagctg ctgaaatcga cggagcgaat
     5521 catttgtata tttggagtca tttgatgctt ccattgacga aacctgcctt gattaccatt
     5581 gcgatcaatt ccttcaacgc agcttggaat gatttcttag gtcctctgtt atatatccaa
     5641 gatcaagaaa aatacacgtt acagattggc ttgcaagttt ttcagaacca agcaacgaca
     5701 caatggaatt atctgatggc aggagcaaca cttgtcttga tcccgacgat tttgctgttc
     5761 ttttttgcac aacgttattt cattgaaggg atggatttga caggaggttc aaaaggatga
     5821 tgaatcgatc gattggtaaa aaagccaatg attatttctt agaaatcatt gaatggattc
     5881 cacgtcttat ccatttacag ctactatggt tcctgtgcgt cttgcctgtt ttcacattag
     5941 gaactgcaag tcgaacagtt ctgtatatga tttatcatta caaaaaaaat gaagagaaaa
     6001 agctcggttc actattctgg aaaaagtttc gtgagaactt ctctctttat ggaaagcaag
     6061 atagtttagc aagtgtttat tttcttcttc tattgatcga ttatcagatt ttccgctatt
     6121 ggggtggggg aattggctat acattgatgt atactacgtt gtttttgctt ctattaagcg
     6181 cgcttctctt tagttacaaa atcttgcttc aactggaaca aaaagaagct tcttggatta
     6241 ctgcattttt cttgtttttt aatgatttca aaagcgcgct tttctatttg gcaggcacgt
     6301 tgatccttct tgtcgcattc tggtttgcag gaccaatcta ttttgcttta ttaggtttca
     6361 gtttcttgtt ttattttcaa gtcctgcttt ttatccatcg tacgcaagaa aaggttctta
     6421 aaaaggagga ataacatgtt gcgtttgatt gctaaaatcg atgatagtat tatcaacaag
     6481 ttcgccaagc aacggaaaac ggatagtcga aaaatcaatt ttttgaaatt tatcagtaat
     6541 atctctaatg gtggttttat ttggttgctc cttgctgctg ttttagtctt gatacctgat
     6601 actagagaat atggtctagc cattcttatc ggcagtttga tctatttttt tgttggtttg
     6661 ctttttatca aaaaaacgac aagacggaaa agaccttatg aaaaaaatcg tcggcttgtc
     6721 ctttacacaa gtaaaccgaa gacttcttct tttccttcag gacatacagc gctagccttt
     6781 ttatcagtag ctgcgttgtt cgaattaagt ccagttgcag gaatattagc ctttttattt
     6841 gcttgtttga tcgcttattc gaggattttt ttacttttac attatcctac tgacatttta
     6901 ggaggagcta ttttaggaag tgttattggc atcctatgcg ctgccgttat ctgaattaat
     6961 tgtacgaata aagcatgagt gcgtaaatgc gcatttgtgc tttatttttt tgtttcgagg
     7021 aaagcgtagt tgtttattca tttgtcttaa taacttaagt tttgttaaat ga
//
LOCUS       DAFFSV010000129         6994 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFFSV010000129 DAFFSV010000000
VERSION     DAFFSV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.129"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            177..1034
                     /locus_tag="LR110_002468"
     CDS             177..1034
                     /locus_tag="LR110_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL4823405.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1027..1929
                     /locus_tag="LR110_002469"
     CDS             1027..1929
                     /locus_tag="LR110_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL4823406.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1962..2312
                     /locus_tag="LR110_002470"
     CDS             1962..2312
                     /locus_tag="LR110_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL4823407.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2361..3284
                     /locus_tag="LR110_002471"
     CDS             2361..3284
                     /locus_tag="LR110_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL4823408.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDASDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3362..4255
                     /locus_tag="LR110_002472"
     CDS             3362..4255
                     /locus_tag="LR110_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL4823409.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4293..4856
                     /gene="amaP"
                     /locus_tag="LR110_002473"
     CDS             4293..4856
                     /gene="amaP"
                     /locus_tag="LR110_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL4823410.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4870..5058
                     /locus_tag="LR110_002474"
     CDS             4870..5058
                     /locus_tag="LR110_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL4823411.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5071..5973
                     /locus_tag="LR110_002475"
     CDS             5071..5973
                     /locus_tag="LR110_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL4823412.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6005..6247
                     /locus_tag="LR110_002476"
     CDS             6005..6247
                     /locus_tag="LR110_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL4823413.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6503..6874)
                     /locus_tag="LR110_002477"
     CDS             complement(6503..6874)
                     /locus_tag="LR110_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823414.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2548 a         1189 c         1391 g         1866 t
ORIGIN      
        1 acctttgaat aaaaagccat gaaaaacgaa tagttagcgc ttaccgatgc gttttgtgat
       61 aaaaaaattt taaaatattc aatgaaaaca aagcatatat tttgctttaa aaccaaatga
      121 ggaatattct agatgtagaa caggttagaa aaagattaga aaaaggagtg attttgatgg
      181 agaagagata cggcgtagtt cctgtggtaa ctgcttcaga cgaaaattat gctccttact
      241 tgagcgtgat gatcgcaact gcattagaaa attgcaataa aacaagacgc ataaaatttt
      301 atgtgattga cgatggtcta tcagaatata gtaaagaagg attggaagaa acggtaaata
      361 aatattcaag taatgcaagt atccaattcc tgacagtgga aaaagatatc tacgaagact
      421 tcctagtcag tgaccatatc acaactaccg cttacttaag aatttcttta ccaaacttat
      481 tagctaaaga ggattataag aaagtccttt acttagattc agatgtgtta gtactagacg
      541 atatcgtaaa actatatgat gagccgttaa atggaaaaac aatcggcgct attatcgatc
      601 caggccaagt gaaagctttg gaaagattag gaatcgattc cgatgacttg tacttcaatt
      661 caggagtgat ggtgattgac attgatcaat ggaataaaaa agaaattaca gaaaaaacta
      721 tccattacct cagtgaaaat ggcgatcgaa tcatctatca cgaccaagat gctttgaatg
      781 ctgtacttta tgaagattgg gaacaacttc atccaaaatg gaacatgcag acttcgctga
      841 tttttgaacg tcatccagca ccaaatgaaa aatatgaacg tcagtataaa gaaggaaatg
      901 aaaaaccttc gatcgttcac tttacaggac atgataaacc ttggaatacg ttgaaagatc
      961 atccgtatac caatttatac ttaaaaaaat tagcacacag tactctaacg aaagtaggcg
     1021 agatcaatga ataaaaaaga aatagcagta gtcgcaagtt gtaacacaaa attcgtacca
     1081 catttagctg cattatttgt ctcagtatta gataattgca acccgtctaa atttgttcgg
     1141 ttctatgtga ttgacgatga tatcgatttt gaaagtaaac agcttttacg cttctcagtg
     1201 aaaaacgcac gaatgaactc agatgtggaa ttcttgaaaa tcaataaaga attctttaca
     1261 aatgtggtaa tcagcgatcg tattccagaa acagcttatt atcgtattgc gattccggaa
     1321 ttgttccgcg gcacagaagt tgaacgtatc ttatatatgg attgcgacat gatcgcttta
     1381 caagatatct ctaaattatg gcgtcttgac ttcggtgatt caatcgtagc agctgttgaa
     1441 gacgctgggt tccatcaacg actggaaaaa atgaaaattt cagctaaatc aatgcgttat
     1501 ttcaactcag gtttgatgct gatcaatgtg aaaaaatggt tagatgaaaa tattacgcaa
     1561 aaagtattag atttcatcga acataatcca gaaaaattac gattccatga ccaagatgca
     1621 ttaaatgcta tcttacatga ccgttggtta ccacttcatc cgcgttggaa tgcacaaggc
     1681 tatatcatgg caaaagcaaa aaaacatcca acagcagcag gtgaacgtga atacgaagaa
     1741 actcgcaaca atccttacat tatccatttt tcaggacatg tgaaaccgtg gagcaaggat
     1801 tttgaagggc caacgaagaa atattacgaa aaatatgcag ggatgacggc tttccgttgt
     1861 gtagctaaat tccctaaata tccaaaatat gcaaaagttc aaccaaaaac agatgtagtt
     1921 gttaaataat agaagacaac aactgaagag ggggagtagg aatggaaatt acaatgtcta
     1981 aaaatgcagt agagacgtta attgaaaagg ttggggaaaa tacaaaaatc gcactcgcgc
     2041 ttatcaatga ttccgatcca tttctaaggg acaaaggtgc ttttgcaaaa ggcagctttt
     2101 ttcaaatcat tccttttgtt tcggaatttg gagaatatgc gactaaaatc gagcatccat
     2161 tattggacat ctatacatct aaattagagc aaaattattt cggtaaacga ttgaacatgg
     2221 acttcaacaa acagcttgac agcttttcat tagaaaatga aatcgcagta ctcgattata
     2281 atatcaaatt aaagaactgc ttcttcagct aatagtttcg agaaagtcca aaaaggaggt
     2341 atacattgag atactacttt gtggatgaag aaaagaatgt aatagaaaca gatgaaaaaa
     2401 aatataattg gctcgtactt gatagtgcaa agcccgaaga gattgagcaa gcaatcgaag
     2461 aattcgagct tcctgatgat attttcgtag gaaccgaata tccggaagaa gtttctcgga
     2521 tggagcgcct ttatgagacg aaactagagc atccattttc tttggtagtg attaatctag
     2581 atcagtcaga taatcgtatc gaaaaaaaat taacgccaat atcttttgta ctttccgata
     2641 atctcttgat tacttgtatc gatagaaaaa ccgaatttgt ggatcggctg ctgacaaagc
     2701 atggaaatca tttaaactcg tttgaaaaga tcatcacgta tacattatta gatatttaca
     2761 cccattacgt gaaagaacta agagagatga aaaagcgaat tgacgcttcg gataaagcag
     2821 ctcgtaaaac aacggaaaat gaagaactgt ataaacaggc agatcttgaa agagacatcg
     2881 tatatatcga tcatacctta agagatcaac gggaaacaat ggatcaatta tggaattctg
     2941 aaactttcat caaacgttta gacgatgaac agctactcta tgatgtccat ttgagacagc
     3001 gacaaacaga aaaaatgatc gagatctatc gagatttatt agagagtatt ggtgatctat
     3061 tcaacgggat gatggacaat aatctaaatc atatcatgaa atatctagat tcagctgcat
     3121 taatcatttc cgttccggcg ttgttcgcag ggatttgggg aatgaataca ggaggattac
     3181 cgggtaaaag ttctgctcta ggctttatac tcgtagtctt tggctctttg atagcagcag
     3241 tagttttagg ttaccattta taccggaaag attatacaaa ttaaagaatc tatttaaaac
     3301 aggtatcaaa caaagaaatt tgttttggca atacaattta aaaactagga ggaatacagc
     3361 aatgacagaa ccaaaattaa tcgacccgcg aaacttgtac catacggaga aatttcctaa
     3421 acaagatcag gatacaccag ctttacagca gaatatgatt cctgttcctg attgtggaga
     3481 agaatcctat gaaggcaata atcaactaga aaaccgccgt gtactaatca ctggtggaga
     3541 ttcagggatc ggtcgtgcag cagcgattgc atttgctaga gaaggggcag atatcgctct
     3601 tcaattcttc ccaggagaag aaaaagatgc agaggaagta gcaaaatacg tgaaagttgc
     3661 aggacgtaag attgtcttac taccttttga tcttcgtgac gaacaagcac ctaaagaaat
     3721 catagataaa gccgtgagtg aacttggcgg gctagatacg atggtactaa acgcaggaca
     3781 gcaaatttct tgtcagtcta ttgctgagtt accacttgag caagtcaaag acacatttat
     3841 gataaacatc atcgctatgt ttgctttagt gaaagaagct gttccgcata tcccagcagg
     3901 aggagcaatc gtaacaacaa cttctgttca agcattcaat ccaagcgaac atttattaga
     3961 ttacgcagca acaaaagctt caatcgctaa ctttacgatc ggtttagcaa aacagcttgc
     4021 acctaaagga atccgtgtaa atggtgtagc acctggtcca atctggacac cattacaact
     4081 agacgaagga caaccttctg attcgatccc tgaatttggt cagcaaagtt tactagaacg
     4141 tgcaggtcag ccaggagaat tagcaccagt ttatgttttc ttagcatcga ataaagcaag
     4201 ttatgttaca gcccaaattt acggagtaac tggtggagaa gcgatcaact tataagaagt
     4261 tgatcgtttc acccctatga ggaggtgcga aaatgaataa aggtgtaaaa acgatcggag
     4321 tgatcggaac attacttttg ctatctgtgc ttttgtttgc gatactatta agtgcaacct
     4381 atccattgcc ttttcagcta gagaagtttc gattcttttc catcactaat tcatacgtgc
     4441 aacaatatgt attttggctt gccgttgcat ttgctatttt gacaattatc acgttacttg
     4501 ttttcctgtt ttatccaaaa acaatcggaa actttctact gaaacatggt gatggggagt
     4561 tgacgttgga taaaaaagca attgaaggaa tggttcgttc tcacttgcat gaagatgaat
     4621 tcatccattc accaaaagta aatgtcaaag caacgaaaaa caagatcgcc gtcaatgtaa
     4681 aaggggagtt gaaacgaact tcagcactca ttggtaaaac aggaacattg atgaaagaaa
     4741 tcgaaaatga agtacaacac atattgggaa caaaagagcc agtgaaagta tcggtgaaat
     4801 acacaggctt tgaagaacca gctaaacgaa gcaatgaccg caaacgagta gaataggggg
     4861 ggaccaaaaa tgaaagaact attttcgaca tacaagctcc ctatcatctt tggggtactt
     4921 ggattgatat tagccgtttt atggatcacc gtgggctttt tcaagacctt attgatcttg
     4981 atcatggcag cgatcggcgt cgctatcggt ttttatctga aacaaacgaa aatcttagat
     5041 cactatttta aatcttagga ggaaacatcc atgaataaca ataacaaaaa taataacaat
     5101 aaaatgaaca accaaaaacc agtgtctgca catccagacc caacgacacc aaaccaatca
     5161 aatggtgtga acgatgaaaa gaacaaaaca gcaccagtat ctgcacatcc agatccagta
     5221 gtccctaacg ttcaacaagg agtagaagag aagaaacatg atgcagaaaa gcatgcacct
     5281 catcatccag gaactccaga atctaaagtt ccagaacaac atgatgcaat cgatccttct
     5341 aaacctgtgg aagagcaaaa acatccttct gaccatgcga aagcacaaga aaaacttagt
     5401 tctgaccaca caaacggaca aggaaaactt ggttctgatc atacaaataa ccaaggaaag
     5461 catgattctg aacaggaaga ttcaatgcgc ggtgagctga attatgaaga taaagttgtg
     5521 caaaaaatca tcggaatcgc aattgaacaa gtagacggtt tattatctgc aaacggtggt
     5581 ttcttctcta atgtggcagg aaaacttgtg aatacagata atgtaacggc tgggatcgaa
     5641 acagaagtcg gtaaaaaaca agtagcagtt gatatggatg tcattgtgga atatggcaaa
     5701 gatatcgaaa aaatctttga agaaatgcaa gaagtaatcg gcaaagaagt aaaaaatatg
     5761 acacatttag aagtgatcga agtcaacgct aacgtagtgg acatcaaaac aaaagaagaa
     5821 tttgaaaaag atcaagaaac ggtgcaagac aaagtaactg acgcagcgaa aaaaaccggt
     5881 gaatttgctt cgaatcaaac agataaagta aaatcagcag ctggaagcgg cgcacaaaaa
     5941 gtaaaagaaa atacagaacc acgggttcag taataaggaa gtaaaacatt aggaggaacg
     6001 aaacatggga tttatttggt cacttatcgt cggaggaatt atcggagcaa ttgcaggagc
     6061 aatcacgaat aaaggttctt cgatggggat tattgcaaat gtcgtagcag gtttagttgg
     6121 atcagcactt ggtcaagcat tattaggttc ttggggacca agtattgcag gaatggcaat
     6181 cgtgccatca ttgattggtg ccatcatttt agtactaatc gtatcattct ttgctcgaaa
     6241 actgtaaaaa gacttttaaa aagaggtcgt gaaaaagctg ttcttcatca agtaataaga
     6301 acagccaaac aaaaatagct tctcatattt ttcatttggt tgggcatatt atcgaagatg
     6361 caagcttttg aacactgttt atacggaata agaggctgtg acaagacttt tgtcacagcc
     6421 tcttatttat gcgggcaaat tcccaattag atttttaaaa gtttttcctt aaatgtgtct
     6481 ttattgatac agagaagctt ctttaataaa acaatgcctc cctaaatttt cttgagatag
     6541 gaaaggtgaa catacaaaat aatacgacca ctttgaaaac ccagatgatc cacaaatgga
     6601 tcgtatgata ctgatgaagt aaaaagcaat ggatcagaaa ctcaaagaga ttgaaaaaga
     6661 caaggaagaa ataatatctc ttttttactc ttgtctgttt tagccaataa tagctcgtaa
     6721 agtaatctag acaaacaaat agaagtaaac aaagtttcgt aagagcagca atcgtatcat
     6781 gttgctctgc tagacgattg acgagcagga aaaatgttgg taatggaata tagatcacga
     6841 gtaatgaaag aaaaaagtaa acaataaatg gcatactatt cctcctaatt gtgtgagttc
     6901 gccctcattt taaaataacg atatttgttt gtgaagcttt ttcgtgtcgt aacaaatgtt
     6961 atctaatgta tttttaaata aataatcatt atta
//
LOCUS       DAFFSV010000130         6952 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFFSV010000130 DAFFSV010000000
VERSION     DAFFSV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.130"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..380
                     /locus_tag="LR110_002478"
     CDS             <1..380
                     /locus_tag="LR110_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL4823415.1"
                     /translation="KSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFVIVFA
                     HVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTFAGKK
                     VKEQKKRWDEPITLVRSVNYVPD"
     gene            364..2814
                     /locus_tag="LR110_002479"
     CDS             364..2814
                     /locus_tag="LR110_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL4823416.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWK
                     ETLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGS
                     ITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNRE
                     IFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGL
                     KFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVF
                     EELFHAFDTRPPVQERME"
     gene            2819..4927
                     /locus_tag="LR110_002480"
     CDS             2819..4927
                     /locus_tag="LR110_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL4823417.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            4924..5928
                     /locus_tag="LR110_002481"
     CDS             4924..5928
                     /locus_tag="LR110_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL4823418.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            5947..6858
                     /locus_tag="LR110_002482"
     CDS             5947..6858
                     /locus_tag="LR110_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL4823419.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
BASE COUNT         2239 a         1185 c         1540 g         1988 t
ORIGIN      
        1 taaaaagcta caccagtatt tgggcagttg aaaaagtcat ttatgcgatt aatgactttc
       61 agctgccttt tcctgtgacc tttaaccaga tggcttggtt tgttttatcc ttgatgtttg
      121 tcattgtatt tgcccatgtg cctccgctat ccatgataga gggggctttt ttaaaatact
      181 tgggcattcc gatagcggtc acttggttca tgtcgcaaaa gacctttgac ggtaagaaac
      241 cattgggatt tttacgatcc tttatcagct atcacttacg gtttaaggtc acttttgcag
      301 gaaagaaagt taaagaacag aagaaacgat gggacgaacc aatcaccttg gtaaggagtg
      361 tgaattatgt acccgattaa gtatattgaa aataatcttg tctttaacca agaaggcgag
      421 tgttttgctt actatgaatt agtgccttac aattactcct ttttgtcacc tgaacagaag
      481 tatcaagtac atgataactt tcgccaatta attgcgcaaa atcgtgaagg taagatccat
      541 gccttacaaa ttgccacaga aagtagtatt cgtgccacac aagaacgttc gaaaaaggaa
      601 attaccggta gactcaaaga agtagcaaag caacgaattg atttacagac agatgcatta
      661 gtatccatga ttggcgatag tcagattgat tatcgcttct ttattggctt taaactgatt
      721 tcgacagatg aagaggtcaa tctaaagaat ctcaggaaat ctttcttttc tggacttcaa
      781 gaatttgtct atggagtgaa tcatcatttg atgggtgatt ttgtttcctt atccaatgaa
      841 gaaattcgtc gctataccaa gcttgaaaaa ctaatggaaa gtaaattggc tcgaagattt
      901 aaagtcaaac gagtaacgcc gagtgacttg acgtatttaa ttgaacatat ctatggtgaa
      961 aaaggcacac cgtttgaaga atatgaattc cagctaccaa agaaaaagct gaaatcagaa
     1021 acgctggtga aacgttacga cttattacgt cccactcgtt gtttgattga agaaaagcct
     1081 cggtacttac ggatggaaca tgaaaatcat gagtcttatg tagcgtactt aacaattaat
     1141 accattgtag gggaaatgga gtttccttcc tcggaacttt tctattacca gcaacaacaa
     1201 tttacgtttc cgattgatac ttcaatgaat gtggaaattg tgacgaataa aaaggcccta
     1261 gctaccgttc gtaataagaa aaaggaatta aaagatttag acaaccatgc ctatcaatct
     1321 gataatgaaa cgaactccaa tgtattagat gctttggatt ccgtggatga attggaaacc
     1381 accttggatc agtcgaaaga atccatgtat aaactaagct atgtggttcg agtgagtgct
     1441 aaatcagtgg atgaattaaa acgccgctgt gatgaagtgc tcgattttta tgacgatacc
     1501 aatgtaaaac tagtccggcc ctttggcgat atgatgggtc tacacaaaga attcttaccc
     1561 tcaagtaagc gatacatgaa tgactatatt caatatgtta cgtctgactt tttggcggga
     1621 cttggttttg gggctactca aatgctagga gaattagaag gcatttactt tggctataac
     1681 gtagatacgg ggcgcaatgt ttacctgaaa ccggcattgg catcacaagg ggtcaaaggc
     1741 tcagtaacca atgctttggc atctgctttc cttggttcgt taggtggggg gaaatctttt
     1801 agcaacaatc tcttagtcta ttatgccgtc ctttttggtg ggcaagctgt gattgtcgat
     1861 ccaaaaggag aaagaggcgg ttggaaagaa acgttgccag aaattgcgga tgaaattaat
     1921 attcttaact tgaccagtaa gccagaaaat caaggcttac ttgatcccta tgtgattatg
     1981 aagaaaaaga aagactcaga aagtttggcg attgatatcc tgaccttttt aacagggatt
     2041 tccagtcggg atggggagaa gttccctgtg ttacgcaaag ccattcgaac ggttacccaa
     2101 agtgatcagc gaggattatt acaagtaatc gttgaattgc gtaaagagga ctcaccagtc
     2161 gcggaaaata tcgcggatca catcgagtcc tttaccgatt atgattttgc ccatttgctt
     2221 ttctcagatg gttcgattac ccagtcgatt agtttagata agcagttaaa tatcattcaa
     2281 gtagcggatt tggttttacc agatgcagaa accacttttg aagaatatac caccatggaa
     2341 ttgctgagtg tttccatgct aattgttatt tccacttttg ccttggactt tatccatagc
     2401 aaccgagaaa ttttcaagat tgtggacctg gatgaggctt ggagttttct tcaagtagct
     2461 caaggaaaaa cgttatcgaa taaattagtg cgtgctggtc gggccatgaa tgccggtgtt
     2521 tacttcgtca cgcaaaacag tgatgacttg ttggatgaac gtttgaagaa taatattggg
     2581 ctaaagtttg catttcgcag taccgatatt catgaaatca agaaaacctt ggaattcttt
     2641 ggattagata aagaagatga aagtaatcaa aaatgcatgc gagatttgga aaatgggcaa
     2701 tgcttgatgc aggatttgta tggtcgagta ggtgtggtcc aagttcatcc agtctttgaa
     2761 gaacttttcc atgcttttga tacccgtccg ccagttcaag agagaatgga gtgaggagat
     2821 gaaaaagaaa ctttttcgta tctttggaat tgttttaatc agtttggtca ctctgataat
     2881 acttttgagc ttagttggaa cggtagctga ggcaacaggt ctggttgatg atacagttgg
     2941 agccggaaat ctttattccc aatattcact tggtaactat cagttggatt tctttgtgga
     3001 tagttcgtgg gattggctgc cttggaattg gggtgatggc ctagggaaga gcgtaatgta
     3061 tggactttat gccattacta attttatttg gacagttagt ctctacttat caaatgccac
     3121 gggctacgtg gttcaagaag cctataagct ggatttcatt tcagatacgg ctgaaagtat
     3181 tgggaagaat atccaaacct tagccggcat taccgaaaat ggactccaga cctcgggctt
     3241 ttactttggg tttctattat tgatgatttt agctctagga gtttatgtgg cgtatacagg
     3301 attattaaaa cgagaaacta ccaaagcagt ccgggctgtc attaattttg tgatgatttt
     3361 tctactttcg ggttcgttca ttgcgtatgc gcccacctat atcacaaaaa tcaatgattt
     3421 tagctcagat gtcagcgaag cggctcttag ccttggaacc gagattgtcg tgccgaattc
     3481 agaaagccaa ggaaaggaca gtgtggattt aatccgagat agcttgtttt ccattcaagt
     3541 ccaacaaccg tggttactct tgcaatttga tgattccaat atagaagaag ttggcgaaga
     3601 tcgtgttaac aaaatcctgt ctgtaagtcc agatgaaaat aaaggcaagg atcgggaaga
     3661 agcggtcaaa gcggagattg aagataatga caatgcgaat ctcagtatta ccaagaccat
     3721 gaaccgctta gggaccgtgg tctttttggt gttgttcaat attggcatat ccttctttgt
     3781 ctttctccta acagggatta tgttgttctc gcaaattctc tttattatct ttgcgatgtt
     3841 tttgccgatt agtttcttat tgtccatgtt gccgacttat gagagtttgg gcaagaaggc
     3901 gattattcgc ttgttcaata cgattatgat gcgagcagga gttactttgg tgattacgac
     3961 tgcatttagc atctccacga tgtttttcaa tatttcggca acctatccat ttttcatggt
     4021 tgcttttctg caaattgtga cctttgccgg tatctatttc aaattgggcg acattatgaa
     4081 tatgttcaat ctccagagta atgatagcca atcaatggga agacgggtca tgcgaaaacc
     4141 gcaaatgtta atgaatcgaa cattgcggca actcaatcga aatgtgggac ggactttggc
     4201 ttttggtggt ggtgcagcag ttggaaataa gttggcaaag gaacaatcaa aatccaaatt
     4261 taagccatcc ggttcttctc tacgaaagaa ttcacgatta ccgaacgatc acgagatacc
     4321 atctgacttg aataaggaaa agccaattcc aaataataag aaacaatccc gaatgaattt
     4381 ggccgggaga aaagtcggca aggttctgga tacccaagct ttggtgaaag ataagaccaa
     4441 acaagtaaag gatggagtac ggaatacgcc aaccaacttg aaatacaact tccataaagg
     4501 aattgaaaaa acgaaaaaag caccggaaga gtttaagcga ggactggtcc aagaaaaagc
     4561 aaatcgagga gagttgcgag aaaaacaaca acaacgtaga gacgaaaaaa tagctgaaaa
     4621 gcgcaaagta ttgaacgaaa tagggaatcc tcatgggaaa agaagggaaa atgttccgat
     4681 aaaagcaaag gtccaatctc aaaaggatag agcaccaaag cggattgtga gagcaaattc
     4741 gaatcccgaa atcaaacgaa aattcgctag ccaagaaatc atttcaaaag gaataaacca
     4801 atcctcaagt aagaaaaatt cttttcaaca agtgaaacgg cggtctactc tgtccaaacg
     4861 aaccaatcga aaagtacaaa aaagtttgaa ccctcatccc aaaccaaggt caggtgagaa
     4921 aaaatgaacg taaagaaatt aagtgccatt tcgttaactt tcctaacaat ctcttttatg
     4981 ggtatcttga tgtgtgtggg cttattgttt ggtgaagaca gtgaaggaag tagtagttca
     5041 ggtgctcctc ctggggggag tagtgtctca gaagaggttt tgaaacaccg tgcaatggtg
     5101 gaaaaatatg ccaaagaatc ggatataaca gagtatgttc cgattctttt agcgattatt
     5161 caagtggaat ctggtggcac aatggaagat gtgatgcaaa gttctgagtc agccggttta
     5221 ccaccgaatt cattaagtac cgaggcatcc atcaaacaag gatgtctcta ttttgcgtct
     5281 ttggttaagc gttccaaaga attaggttgt gatcaagata gtatcattca ggcctataac
     5341 tttggcggtg gttatttgga ctatgtggca aaaaacggga agaagcatag ttttgcttta
     5401 gctgaatctt tttcaaagga aaaatcttgc ggtcaaaaaa tcgattaccc taatcccatc
     5461 gcgaataagg aaaatggtgg atggcgctat aactatggca atatgtttta ttgcttattg
     5521 gtgaagcaat acctaactac aacacagttt gacgataaaa cagtccaagg gatttttgac
     5581 gaggcgttta agtatgaagg aacggcttat gtatttggtg ggtcaagacc cgaaacagga
     5641 tttgattgta gtggcttgac tcaatggtgt tatgccaaag ttggtctgaa acttcctcgg
     5701 gtggctcaag atcagtatga tgccatgacc catattgacc taaaagatgc caaaccaggg
     5761 gatttggttt tctttcattc gacttatgat gccggtactt atgtgaccca tgtcggtatt
     5821 tatgcagggg agaatcgcat gtttcatgca ggagatccag taggctggac gaatctaaca
     5881 gaaagctatt ggcagcagca tcttatcggt gctggacggt acaaataaga gaaaagagga
     5941 aaaagaatga agataaaaat tgaacggaat caaaaggaaa agagcctaaa aaagaaaaaa
     6001 gaacgagtcg tatccgtggg taagcatcga aaaatggtgc ttgccctttg gcttttatta
     6061 tcttgtagtc ttacttttgg cgtttataaa aatttcaccg ccattgatca acatacaact
     6121 catgaaaaag tggttattaa agagaaagta gtgaatactt ctggtattga aaactttacg
     6181 aaggattttt cgaaagaata tttttcgtgg aaaaataaca aagaagcaat tgaaaagaga
     6241 atgaacaacc tcgaacagta ccttacagaa gaaggtttcg ctttaagtca agatatggtt
     6301 cgagcagata ttcccactaa ttccgaagtt cagtcagtaa aagtcttaga tgttgaaaag
     6361 agctcggagg aatttgtcgt ttcattttta gtggagcaaa agattacaga gggaaaaaag
     6421 gcacaatcga tttcttctgc ataccgagtg acgatctttg aagatgaaaa caggaattat
     6481 atagtaacga gtttgccaac tatgattgca aaacctgata gggcaaaata caagacaaaa
     6541 caagtagaaa atgattcgaa aattgatgcc aaaactacag aagaaatcgc tgagtttttg
     6601 gaaacgttct ttaaattata tccatcagct tctgaaaaag aattggagta ttatgttgaa
     6661 aatgatgcga tgcgaccaat caatattgac ttaaagtttt cgaagttaga ggatccagtt
     6721 tacctacaaa acggaaaaaa aattctagtg agtacaaaga taaaatacct tgataatgta
     6781 tcaaaaacca ctagctactt tcagtacaaa ttaatattac aaaaaaatgg aaactggaaa
     6841 atcattgatg ttaaataata atgactcaat ctaaatttat gagaagttat aagtagttta
     6901 acttatttca ggcagaagtc gcccatcctc tataggtggg tgatgaatgc cg
//
LOCUS       DAFFSV010000131         6931 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFFSV010000131 DAFFSV010000000
VERSION     DAFFSV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.131"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            1..261
                     /locus_tag="LR110_002483"
                     /pseudo
     CDS             1..261
                     /locus_tag="LR110_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400658.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maturase"
     gene            381..872
                     /locus_tag="LR110_002484"
     CDS             381..872
                     /locus_tag="LR110_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL4823420.1"
                     /translation="MKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKS
                     LQSLVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLP
                     KSLGGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQ
                     NIQ"
     gene            998..1354
                     /locus_tag="LR110_002485"
     CDS             998..1354
                     /locus_tag="LR110_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBL4823421.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            1399..2541
                     /gene="dcm"
                     /locus_tag="LR110_002486"
     CDS             1399..2541
                     /gene="dcm"
                     /locus_tag="LR110_002486"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HBL4823422.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            2531..2820
                     /locus_tag="LR110_002487"
                     /pseudo
     CDS             2531..2820
                     /locus_tag="LR110_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3076..4260
                     /locus_tag="LR110_002488"
     CDS             3076..4260
                     /locus_tag="LR110_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL4823423.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            4257..4370
                     /locus_tag="LR110_002489"
     CDS             4257..4370
                     /locus_tag="LR110_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL4823424.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            4399..4620
                     /locus_tag="LR110_002490"
     CDS             4399..4620
                     /locus_tag="LR110_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823425.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            4633..5136
                     /locus_tag="LR110_002491"
     CDS             4633..5136
                     /locus_tag="LR110_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL4823426.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            5262..6299
                     /locus_tag="LR110_002492"
     CDS             5262..6299
                     /locus_tag="LR110_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823427.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            6314..>6931
                     /locus_tag="LR110_002493"
     CDS             6314..>6931
                     /locus_tag="LR110_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBL4823428.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKK"
BASE COUNT         2267 a         1079 c         1564 g         2021 t
ORIGIN      
        1 ccaatgagaa gtctaaaatc gtcaatttgc gtaaatgtaa gtcggaattt ttaggattcg
       61 ctatttgcgt gaaacaaaaa gggaaaaaat gggtttgtaa ttcacgtatt tctaacaaaa
      121 aaaaggatca gattaaagaa gagattaaac aaagaatcaa agatattcaa aagagtccta
      181 cagcacaaaa tgctctcctg tttaatgctc tcgtgctcgg tgttcataat tatttcaggt
      241 atgccactca tattaattta gatttgaata ggatgaacta cgttctttca ttcactactt
      301 acaaccgatt atcaaattgt agtacaagat gttaccccaa caaagcttcc ccaacttaca
      361 aaaagtttta ctcaacaacg atgaaaacgt ataaagtagc tggagtctat ttgtaccctc
      421 tttgcgacgt atctacaaaa acaatttacg gatttaatac ggaggataca ccgtttactc
      481 cgtttggtcg tcaaagattg gagcacaaaa gcctacagtc tcttgtgttc caagaattaa
      541 gaaaattgat ggaatcgaaa ataccgaata gaacggttga gtatttagat agtagaatat
      601 ctcgttactc aatgaaatca ggcaagtgtg agattacaaa acaatttttg ccagcaaaag
      661 ccgttcactg tcatcactat ttgccaaaaa gtcttggtgg agacgacaaa ttcgataact
      721 tgagaattat tcataaggat atccatcttc tgatccatac aacaaataaa atgataatcg
      781 atcactacgt gcatgaactg aaactggtgc cagagcaaat agcaaaaatc aacctttatc
      841 gaaagatgtg taatctgcaa aacattcaat agaaaataaa tagatggaac gccgtatgag
      901 gtgaaagtct cacgtacggt gtggagcggg ggaaaagatg gagattatat caaattctta
      961 cctatcgcta tagatgttaa cgacaaaaga aagtgcggtg attttgaata agcttaaaca
     1021 aatcgtgatg ttaggccgtc aatctggttt ctttctgatt ctagcttgtc aaaggccgga
     1081 tgcgaaatat ctaggagatg ggattcgcga tcagtttaac ttccgtgtgg ctttgggtcg
     1141 tatgagtgag cttgggtatt ccatgatgtt tggagaagtc gataaaaatt tttttatgaa
     1201 acgcatcaaa ggacgaggtt atgtagatac agggggcagt gtgattagtg agttttatac
     1261 gccacttgta ccaaaagggt atgatttctt aagggaaatt agcaacattg ttggattaac
     1321 cgttcatacg gaaagagaaa ataatagtgt gtagcctagg aatatcaata atcctgggct
     1381 tttttattga ggtgatttat gacattttta gatttattcg ccgggattgg tggctttcgc
     1441 ttaggtatgg aacaagccgg tcaccagtgt atcggttttt gtgaaattga tgaatttgct
     1501 cggaggagtt ataaagcaat ccatgataca aggaaggagg tggaaatgca tgacatcaca
     1561 agtgtatcag acgaatttgt tcgatccctc ggaccagtgg acatcctttg cggcggcttt
     1621 ccgtgccaag ctttttcaat tgcgggaaag cggcaaggat tttccgatac tcgaggtact
     1681 ttattctttg aaatcgctcg gttcgccact cttctccaac ctaagtttct attccttgag
     1741 aacgtccggg gattgctcaa tcacgaagga ggggctacgt tcgagacgat cctccgaacg
     1801 ttggatggat tggggtatga tgtggaatgg caagtgctta actcaaaggc ctacgttccc
     1861 caaagccgtg agcggatctt ccttatcgga cattctcgag atgcatgtac cgaacaagta
     1921 tttcctatca ttggatcgtc ttcaacatct gatcaaaaca tcagaaactt ggtaaatatc
     1981 aatccttcca atcgaggaat gggtggccaa gtctatggtt cagatagcgt agcaccaacc
     2041 ttgaccggtg atgaaggaat taaaattgcc ttaccggtaa aggatgggat ttctgtagcg
     2101 ggtatgttgt cgggaaattt tgaacaaggc aataggattt atgaggtaac cggaacagcc
     2161 cccactttgt caacgaaaca agggggaacg aagattatga ttcggaaaaa tgaaacagaa
     2221 tatcaagagg tgaagccagg ggacagtgtg aacctggctt ttcctaattc cacaagtcgc
     2281 cgaggtcgtc tgggaaaaca aagtgtgcat accttactaa caggagacca acaagcggta
     2341 gtcacggatc agtaccagat tcgaaagtta acacctaggg agtgctggcg gcttcagggc
     2401 tttccagatt gggcttttga tcgagcttct caagtcaatt cagatagtca gttgtataaa
     2461 caggccggaa actcagtaac agttccggtc atttttgata ttgctagaag attaaagggg
     2521 gtaaaagcat atgatttatg acgagttgat tggagaaatc tactgggtga ttggcaagat
     2581 tcaggcagat ccgaaattgg aagaagaact gtaccggctc aattttgata ttcgcaaaaa
     2641 cggggtgaaa gtccaggtga ttcgtatgta gtggacgagg aaaccgatgc tcgaatggag
     2701 ctcaatcaag tcatcggtga attggaacga attgcggacc ttgccaaaga acctgatatc
     2761 cgccaatact tgtttgagat aaaagcagag ttggaaattg agggtatcac cgcggagtaa
     2821 tagggaaata acccactgat aggcactgac cgcgggggat ttgcgaagca gaaatcctcc
     2881 gtggtgctgt gcctgttcat ggacggcggc agccggccat gatgtctcac cccccgtatc
     2941 taacagaggg gtacaaaaac acaaaaaaca agcaagtaac cataaaaaat gcttgttgta
     3001 tcaaggatat taagtaattt gtataggtgt taactcattt ggaaaagtta acaccttgaa
     3061 tttggaggtg aaaacttggc gcaacgaaat ttagattatc ggttattaaa agaacgacgc
     3121 aacgaatatg gcgtttcaca aaataaatta gctactgctt gcggacttag ccgcccatac
     3181 ctaaatcaga tagaaaacgg tggcgtaatc gcctctacaa agaccatgag aaaaattttt
     3241 gatcaactgg aaagtttcaa tcccgattta cctttaacgt tactatttga ctatgtaagg
     3301 attcgctttc ccacaacgga tgtgcggaaa attattcaag aaattctcca tttgaaattc
     3361 gagtatatgc ttcatgaaga ttacgccttt tactcctatc aagaacaata tgtcatgggc
     3421 gatattgtag tgatgttatc tcatgaagaa gataaaggtg tccttttgga attaaaaggt
     3481 cgtggttgcc ggcagtttga aactttttta ctcgcccaaa agcgtagctg gtacgacttt
     3541 tttgaagatt gtctaaaagc cggtggcgtg atgaaacgct tggatttagc aatcaatgat
     3601 cgagtggggc tactggatat tcctgattta acgaaaaaat gtcagaagga agaatgtatc
     3661 tccttgtttc gtaccttcaa aagttaccgt tccggggaac tgttgaaagc tgatgaaaag
     3721 gatggtatgg gcaatacctt atatattggc agcctcaaaa gcgaggttta tttttgtttg
     3781 tacgaaaaag attatgaaca atatatcaag ttagggattc cattggacca gaccgaaacg
     3841 aaaaatcgtt ttgagattcg gttgaaaaat gaccgtgctt attatgcgat tcaagatttg
     3901 ttaaaaggtc gtagcattga aagtaccacg ttttccatta tcaatcggta tttgcgattt
     3961 gcggataaag tagatggaaa acggcgaacc aattggccct tgaatgaaca atggggtcgt
     4021 tttattggac ggaatcggaa agagattcaa ttaacttctg aaccgaaacc gtataccatt
     4081 gaacgaacat taaattggct aggtagacaa gtcgccccta cgtggaaaat ggctaaggag
     4141 ttggatcgat tgaatcaaac aacgtatatc caggatatgg tacagaatgc gcgactctcg
     4201 gaccggcata aaaagatttt ggagcaacaa agtatggcaa tcgaaaactt gattgtatga
     4261 aaagaggaat tttgatggaa attgtaaaag atatgttatt ggtacttggt ggcagtatgc
     4321 ttggtgttac cgtgatgtgc ttgatgaatg caagtgcagc agcggattga gcaatggaaa
     4381 agaaaggaaa gtgacaaaat gaatttcgga caaaatttat atcaatggtt tttaaccaat
     4441 gctcaatctt tagtcttact agcgattgtg gtcattggtt tgtttttagg atttaaacgg
     4501 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
     4561 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
     4621 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
     4681 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
     4741 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
     4801 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
     4861 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
     4921 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
     4981 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
     5041 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
     5101 gttactagtc gtggagtttt tgagtatcca gaatgaatca acactatata tagtgtttga
     5161 ttagtatatg taagcaaaaa aacacaatat atagagtgca aactgaaggg taatgtgata
     5221 taatatttgc cgaagaaata tttttgaaat gaggtaatat tatggcaaat attcaaacaa
     5281 gttttattga tttccataat tcaattagat tggacgtaga agacaatacg ctacttaagg
     5341 actataaaga ccaagtaatt gatggattaa aagattactt acctgatgat gtgaagtttg
     5401 aaactttttt acagggaagc tattctgttt ataccggaat aaaaagctgt gatgaaaaaa
     5461 ttgattttga catcgatatt gcagttgctt tcgaaattga tcatactgtt tatgaagatc
     5521 caagggagcc taaactgtgg gtaaaagagg cgctagttga aatttttcct aatgctcaag
     5581 ttaatttaaa agtaccttgt gtaactgcta catttactgg aaaaaagaca aaaaaaaatg
     5641 tgcacgttga tgttgcggtt tatgctaaag aagatgaaaa ttatttctta gcaaaagcaa
     5701 aagaattcag tgctcccgaa aaccgatgct gggaggaagc ggatcccaaa gtattaaaag
     5761 agaaaattaa tagtcatgta gcagattcag atgatcgaaa acaatttcga agatgtattc
     5821 gctatttaaa aagatggaaa gataacaatt ttaaccaaga atataaacca acaggaattg
     5881 gacttacaat taatgtgatg gatacgttcc tagttaataa gtccactgat tttttaacta
     5941 gaaaagttca gtataatgat atggaatgta tgaagcagat tgtttcttcg ctgaaagatt
     6001 catttgtgta tgagtacagt gaaacagatg gttggcatta tcgtctacat gcatatcttc
     6061 cagtgaagcc taacagtgac acctattcaa aaatgacagt gaatcagatg agtgatttta
     6121 aaaacaaact ttctaaatta tatgatgatt tgattttcgc tatcgatact gaagatgagt
     6181 atgaagctac aaaacgatta aataatcaat ttggggagga ttttttaatt atttcagaag
     6241 aagaagttac agaaaaaaac ttaagaaatg catttgttac agactatccg agtgcttagg
     6301 ttggtgttaa agattggatt atactgatat tttaaaagaa aagctaacag atgttgtgcg
     6361 tttattcgat ggtaattact atggggattt ttcatttgct acaattggaa agttgcgttt
     6421 aaaaattgaa ataccaaaac attttccatt ggtacttcct aggatttaca ttcataattt
     6481 tgatcattta ggactttacc taccacatgt agaaaaagat gggttggtat gtttttcttc
     6541 agatataaat tatttagtag atgaagaaaa ttcagaattg gttttattgc agtgtgttaa
     6601 taaaactata aaaacaattg aggaaggttt gaaagggtta aataaaaatg attttcgaaa
     6661 agagtttgtt gctttttggg acaaacaaga gaatcaaatt tcgttattct acctttgtga
     6721 accagcgttg tttatcaagt cagttgatac aataatgtac ggtaagtctt tggttgcttt
     6781 ggataaagag aacgaattac aattaaatgc agcgagtaga ttgtttaatg gagagaaaga
     6841 agatactcat gaattagatg catactatgt tccaattaga aacaagaata atattatgcc
     6901 cccgaagaat aataaagcat ggtcaaagaa g
//
LOCUS       DAFFSV010000132         6848 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFFSV010000132 DAFFSV010000000
VERSION     DAFFSV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.132"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(88..1854)
                     /locus_tag="LR110_002494"
     CDS             complement(88..1854)
                     /locus_tag="LR110_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4823429.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1814..3568)
                     /locus_tag="LR110_002495"
     CDS             complement(1814..3568)
                     /locus_tag="LR110_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4823430.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3678..4247)
                     /locus_tag="LR110_002496"
     CDS             complement(3678..4247)
                     /locus_tag="LR110_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBL4823431.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4265..5680)
                     /locus_tag="LR110_002497"
     CDS             complement(4265..5680)
                     /locus_tag="LR110_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL4823432.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5691..6359)
                     /locus_tag="LR110_002498"
     CDS             complement(5691..6359)
                     /locus_tag="LR110_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL4823433.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6490..>6848)
                     /locus_tag="LR110_002499"
     CDS             complement(6490..>6848)
                     /locus_tag="LR110_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL4823434.1"
                     /translation="SWRENFNELDPFINYIFENKRIFDLVLFQSAGTKYQDIVEKITS
                     FVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLT
                     RRLYQFSVPGWIELLS"
BASE COUNT         2332 a         1246 c          912 g         2358 t
ORIGIN      
        1 ttagatgctg tttaataaaa ttattctttt tgaaaaagaa ttataactat atcccaaaaa
       61 ttgtaggtta atttaaagcc tatttcatca tcgtgattga gcttcttttt catagacgaa
      121 tttctcagat agtttagttt tctttatgag atttttgtat atttctgacc ttcgatacaa
      181 ctcactatga gtaccttgat caactatttt tccttgatcc atcactacaa ttttatttac
      241 cttttcaata gacttcaagc gatgcgatat aattagtact gtcttatctt gtattaaacg
      301 atttaaactt tcctgaatac gtttttcatt ttccgcatct aaactggcag taatttcatc
      361 taaaattaaa attggcgcat ttttcaaaaa agcccgtgct atggataggc gttgtcgctc
      421 tcctccagaa agtgaacttc cgttttctcc gaccaatgta tgataaccgt caggtagttt
      481 ttcaataaag tcttcgcagt tagctaaacg agctgctttt ttcacttcct catctgacgc
      541 ttgactattt ccaatacgta tattttccaa aatacttgta ttaaacaaag ttacctcttg
      601 aaaaacaata gcgattttgc taaacaaaga agctggagat acacgtttaa tatcataccc
      661 atcaattaaa atacagcctt catcataatc atacagtcta gacactaatt tcaagatact
      721 agttttcccg gaaccactag cacctactaa cgcagtcact tctccttgtt tagccgtaaa
      781 agagatatga tctaaaattg gggtattgtt atcatatgag aaagagacat ctcgcaactc
      841 aatgtcaaaa gatttcagag gactatctga tccctcttgg atactggttt ccttcatagc
      901 tctaattcgc tgtattttag gagctaaata aaatatttca agcactgctt ctttcataga
      961 atcgaaggaa tcctttattt ttattgcggc tagtaagtaa ccaacgacgt agagtatagt
     1021 cacttcaccc gttaacagta agtgaacacc tactagaatg actactgcta aactaacata
     1081 tgagaaaata gaggataagg ccatgactga aaaagtactt aattctacct ttaaatgaat
     1141 cctttcactc tcttccattt ttttgaaaag tctgtcttga acttttttag atagattaaa
     1201 gctattaatc tcttgctgca attctatagt ttcttgaaat tcttccgagt tttctctcaa
     1261 agtatcgtaa tatttagtat tggctttcgt ttgggatttc tttgataaca agattaagac
     1321 aaaactaaat aacgttggca aaataacagc taatcccatt ttgacattac caaccaaaag
     1381 catcactgaa ataaaaggga aaaacagagc cataccgccg gctttaggta ttgcatgact
     1441 catcgcatgc tcaatacctt caacgtcaga catgatagtt tgagataaat ctgataaatt
     1501 atgttttgaa aaataggata atggtaaatt tgacaaatcg tctgcaattt gcattcttaa
     1561 atgcgcactt tctttataag tactattata taagttttca tactccctat ctaacaacca
     1621 atacatgaaa agcaaagtaa cgaacgatac tgtaaaataa agccaaggcg gctttacatt
     1681 ttctaaaact aattcgtcta tcagcatcat tataatgaat gctggtacca tattaatgca
     1741 gtatacaaaa aagctagaaa tagatgcttt aaccaaatct tctgcgccct gattagttaa
     1801 agcgaatttg tttttataaa aacttttcat tgtcaagcct ccaatcattt gcattctcat
     1861 aattagtgac caattgttta tacacttgat gtttagccat taattcatta tgatttccac
     1921 gttctactat tttcccttct gacaagacaa gaatttcatc tacagattta atgctggata
     1981 atcgatgagc tatcataata actgtttttt ctttcatcag atgttgaaaa gccttttgta
     2041 gttcatactc attatctgga tcaatagatg cactggcttc atccataata acaatctttg
     2101 catcttttaa aattgctctg gcaatagcta tacgttgttt ttctcctcct gacagataaa
     2161 ctcctttact accaattaag gtatgctctc tttcgggaaa tttttctaaa atcgattcgc
     2221 aaccggctaa tcttagcgcg tcaaatactt tacttgctgg cgcttgagga tttgctatag
     2281 aaacgttatc gtaaatactc tgattgaata gtttcgtatc ttgaaagaca aaagcaattg
     2341 tgttaataat tgtgtctttt gaatagtgat ttaaaggctt accaccaatt ttaatctgtc
     2401 ccttatccac attgtagaaa cctgaaatta gtttagctat cgtactcttt ccgcttcctg
     2461 attttcctac taatgcataa attctttttt gttctaaaac taagttgaaa ttttctaaaa
     2521 cacactcttc tttgtatgaa aagctgacat tttcaaatgc aatgtcacaa tttttaatct
     2581 gtgtctcgtt cccatatttt agttggtctt gcttcatttc taaataaagg gagtctagtg
     2641 tttcgactgc atatcttgct tcaaacaagt gcatacttac atacattatt ctcatgaaag
     2701 aaacaaaaat aattccactt aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg
     2761 gagtggtaag agaagttata aaaaaggtta taggaactat caaaatgcaa actatgccaa
     2821 aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact caaagaatac ttatatgcaa
     2881 acttagcata atcgttaatt gctttattca aggctttaaa tgatgttact ttagcaccaa
     2941 atatctttat aacttgaatt cctcggatat attctaccgt ttcagagctt aacacatcta
     3001 acgattcttg atatttttga ataaaatttg tattctctgt cattttcttt aacaagaata
     3061 caccaattac taacatggcg attagaacca atccaatttt aaaattaatg aaaaagccta
     3121 aggcaactga taaaacaggt gttactatgg cttgagcact atctggaatc aagtgagcaa
     3181 ttgccatatg tgttttagcc gcgttgtcat caattgtctt tcgaatatat cccgaagaat
     3241 ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct aattcccttt tttctcaaat
     3301 tagtctctaa gcgaaaccca aggatatgag aaaataagcc agaaaaaata taaaataaag
     3361 ctcctaaagt taaccaagca acagtttgaa ccgcataact cttagctaaa tattcatctc
     3421 ctacaataat tagagactta agaaatttgt aaatactgta atatccatag actgttaata
     3481 cgacagaaaa atcagaaaat aaagtggcta accatccgta gtatttcatt ttagggacat
     3541 aactaaataa ttttttgtat actttcattt tttcctcccc cgcattcaat ttcttctctt
     3601 attcgtttac aactgttaat gctttccgta atagcgtaaa ttagagatgt acaaacagat
     3661 agaaggtaaa ctacgttcta ttttttgtat ttttttaaga cttttcttcc aatcaataca
     3721 ccaatcatag ataaacttgc tacaactaac actgaaatga ttccccatat acctaccgca
     3781 tagtggatca ttgcatccat tgttgcagca gattgtcctt tatctatcca atcttgacga
     3841 aaacgatctg taaagaacca tataggaatc cactgtccgg cagcactgcc taattgcatt
     3901 agagcccaag acgcaacttg aggaattgct cgatcatatc caaatacaga tagaataata
     3961 tcagctaaga tcgccatact aatcgaactg ataaaccaag gtaagtacgc cccgcccatt
     4021 aacgtaaaaa ataacaataa aacaattgtg taaatagtaa aaattccttt tctagggtag
     4081 ttatagctaa tataaacaaa tatgattcca gataataatc caaaaatccc tggggatatg
     4141 ctgtgaccaa acgccccacc aatactacta accattattg aaaaaaagta aattactagg
     4201 gaaataactg tagctaaacc aataaaaata taatcttttg ttttcatata tattctcctc
     4261 aattctattt cgttatgtca ttaaaaatct gttctatcaa agttgaatct tctactctaa
     4321 gttttatatc tttttttatt ccaccatttt ttaaataaat aattctattg catgttttat
     4381 aaagaaattc cggatcatga gtggctataa ctactgcacc cttttctttt aagtctttaa
     4441 taaggtcagc aatcctttcc atattaatat aatccaaccc acttgtaggt tcatcaaata
     4501 gaaataaatt agtttttgac aatgaagcta tagaaaccaa taatctttgt ttttctccac
     4561 cggataaatc aaacggtaat tgatctttca ttttataaag ttccattttt tttaaaattt
     4621 tttcttgctg atctttacta actatttcat tccccagggc taactcgctc ctcactgatt
     4681 ctgtgaaaaa ttggtaatcc atttcttgca ttactaaaaa cggcgatgac aaattttttt
     4741 tattttcgat tatctttcct ttggtagggc gaataatctt catgagtatt cttagtaagg
     4801 ttgttttccc cacaccgttt ttccctacca aaccaataac atctccaact ttgacatcaa
     4861 aatttatgtc ttttagaata ttttcataag taatttcttc aacttttaat acatctgcac
     4921 ttaccttttc gatataggga acttcaatcg aactattaaa aatgtcaaat ttccttaatg
     4981 cgttattgtt ttcagttttt aacaactgaa tagttgtagt ttttacagtt ctatttttaa
     5041 caaatagaac ttgatcaatt aattctttca aatagtaaaa acgatgttca gctataacaa
     5101 tagtaaatcc tttatttttt aaatcacaaa gtaagtcagc aagtttctta gtcgctttga
     5161 agtccaaatt tgaagacggt tcatccaata taattaattt agagtttagc gataaactac
     5221 atccaataga aattttctgc cgttcaccac ttgatagcga aaaaatgttt ttatctaata
     5281 tatttgaaag atctaataat tcagctaatt cattgattct tttatccata tgctcttttg
     5341 taaatccaaa gttttccatc gaaaatacta actcactcgt tgtatttgtt gtaaagaatt
     5401 gtcctctagg atcttgactt acgtagccaa tattttttga tatactttct tgtgttaagg
     5461 attgtagcga gttattttca aataaaattt ccccaaccaa ttttccgtca taaagtcttg
     5521 gaataagtcc attcattaac tttaatagtg tggatttccc actcccagaa gaaccagtaa
     5581 tcaatgtaag actacctttt tttattgaaa aattaaggtc aacaagcgct tgttccttaa
     5641 catccttata tgaaaaagaa acattgttaa attcaaacaa ttctttcacc tcaaatcaaa
     5701 atacttaaga aaaaaatagt aacggaaaac aggatgatat agtctaataa gctcatgttt
     5761 gttttatagt aagaacttct tttcacagga gattctatcc cctttgttat tgcattagcg
     5821 gtcatttctt cagcaatcat agttgcacgt ataagaacgg gaactaacag aaattccaaa
     5881 tattgcagag gctgaaaaaa agacaagcga atgtttctca tttttaaaga ttctttaatc
     5941 attcttattt cagaacgata agtcggaaag aatctgatca ctactgtaat acttaacatt
     6001 atttttttcg gtaaatgtat tttttctaaa gaggataata attcattgct agtatagaca
     6061 ctaaccaaca accatgagat cattacgacc ggcaacattc gaaatacaat atataaggat
     6121 gtcccaaaaa atatagtaaa aaactctatc ttgatagcat caataatttt cattactaaa
     6181 aacagaactg aataaatcaa gatgaaataa atacatgtct ttattttacg aaacatcaat
     6241 actaaaactg tagcaataat gaaacacccc acttgaaata tatttccctt tcctaaaaaa
     6301 ataatgaccg gaaaaaaaat atttaaagta atcaaggtta acggattgat ttttgacact
     6361 ctttcacctc caaaaaataa gatcgcactg ttatcttaag ctgataaaaa tgcaccgtta
     6421 ttcagataac agtgcgatct tatcaaaaag cataactgag aacaattctc aatgcaagct
     6481 tttttttaat taactcagta attcaatcca cccaggaaca gaaaattgat acaatcttct
     6541 ggttaatagc atagtctttt cttttgaata gctatgcttc aatatatcat agaatgtagc
     6601 gtagaatgag taaatgtaga agtggatttc ttcttcatta aagactaaat tttgattgat
     6661 cattccgata gtctttaact tatctagaca acagactgtc ttttttgtca caaatgatgt
     6721 aattttttca acaatatctt gatatttggt tccagctgat tggaataaaa ccaaatcgaa
     6781 tatacgttta ttctcaaaaa tataattaat aaatggatct aattcattga agttctctct
     6841 ccaacttt
//
LOCUS       DAFFSV010000133         6840 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFFSV010000133 DAFFSV010000000
VERSION     DAFFSV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.133"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(718..3867)
                     /locus_tag="LR110_002500"
     CDS             complement(718..3867)
                     /locus_tag="LR110_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL4823435.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3881..5125)
                     /locus_tag="LR110_002501"
     CDS             complement(3881..5125)
                     /locus_tag="LR110_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL4823436.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(5118..6710)
                     /locus_tag="LR110_002502"
     CDS             complement(5118..6710)
                     /locus_tag="LR110_002502"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL4823437.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
BASE COUNT         1906 a         1406 c         1109 g         2419 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccctcc ctaaacaaac atcttttgta
     3901 agaagccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga gcgatcgtgt
     3961 cgtcgagttg tttgaagaaa gcgccgattt tttgttgttc ctctttattc ctagtaaaag
     4021 gaaacttgat attagcgaaa ttctcataga ataagtattc tcgtacgctt ccttcagtgt
     4081 tgcgcttgac ctctttgata aatgttggtg acttgataaa ctgaagtatg aactcattat
     4141 ctaagtcctc gctcattttc acaactacat aaagagagct aacaatgacc gtcataccaa
     4201 gattgttaaa tgcaattgat cctacgttta ctcttgctgg attataggcg aattcatttg
     4261 gttcaacgaa tttataattt gttttttcta aatcatccaa cctactacca tcaaactgtt
     4321 ctgtttgaga aattaaacct gctttattac ttaccgaata ggcagggaag tctaaatctt
     4381 tcgtgttctt ttttccagtc ttcttactaa actctttgaa cttacgctgt tcccaatctt
     4441 ccgtaaatcc aggaaaccga atttctggca cttttgctcc gtttttaggg aacatttttt
     4501 gtaagaatcc ttttttcgtt tcctttaata aatctagctt acgttgttga agagcgatag
     4561 tgtcgtccag ttgtttgaag aagttcccaa ttttcttttg ttcttctaat attggaaatt
     4621 gtactatcca ttttttcact tctgacattg gtacgagatt tgtaattgct gaacctggat
     4681 tagctcctgt gagttttctt tgcataaaat ctgcacgaat aaattgaata aaatattctg
     4741 aatctgtgat ttctttattt attctaagtc taatcaaagc ctgattgaat acacccggtt
     4801 ttatttgctt tggtactctt gaaattctcc caattgttcc agcgccactc atgataaaat
     4861 cgcctggttc taatttaaat ttgattaatt cattatattt tttttcagta attttatatc
     4921 ttgtttcgta attatcataa atagcatttt gttgttcata gacaacaaaa ttgctattag
     4981 atacaaagaa ttctttcttt aaggcactcc caaatggtcc cctccttact ccatcttttt
     5041 tatcaagtaa gcattcaaac ttacgctgtt cccaagcttc cgtaaatcct gggaaacgaa
     5101 tttctggttg tatatcatta ctcatgtttg aacacctcca gcgcaccttg taaccacgct
     5161 tcattttcag gtgttgtttg cagggaagag atagcttcta aaagttcttt ttctagcact
     5221 tgctgttctt gtcgtatctt cttgatgtca tttccgacat gaaccatatc aattggttct
     5281 tcttcttcaa acgtatctac atagcgtgga atatttaagt tatagtcatt ttctttgatt
     5341 tcatcaaagt ttgccaaatg agcgtatttc tcgacatctt ttctttcagc ataggtttcg
     5401 aggatttttt gaatgttttc ttccgataat ttattctgat tttttccttt gacaaattct
     5461 cgactagcgt caatgaataa tacgtctcga gtttggcggt tctttttcaa tacaatcacc
     5521 gtcgttggaa tcgatgtccc gaagaacaag ttcgcaggca taccgatcac agcataaata
     5581 ctgccgtctt ctagtaattt ttgacgaatg accccttctg cagcgccacg gaataaaact
     5641 ccgtgtggca agacgatggc catagtccct gtttccttca aatgatagaa accatgaaga
     5701 agaaacgcaa aatctgcttt ggattttggc gctaattttc cataacgatt aaaccgagaa
     5761 tcgtctaaga aggtcgtatc tgctgaccaa ttagccgaat aaggtgggtt cattactact
     5821 gcatcaaatg tgtaaggttc atctgttggc caatccttat tcaacgtatc cccattgcgg
     5881 agattcattt cttcggcatc taccccatgt aaaatcaagt tcatttttgc tagattgtat
     5941 gtcgttgtat ttaattcttg cccatgatat ttaacgttat ctgggtgagt taagtagtta
     6001 cgcacattta acatcaatga acctgaaccc atggttggat caaaaacact aaagaatcgt
     6061 cgttcttttt gatccaacgt cacgatttgt gccatcatat cagaaaccat atgaggcgtg
     6121 taaaattctc cggctttctt ccctgcttct gaagcaaatt gactgatcag aaattcataa
     6181 gcatccccga tcacgtcccc atcgtgacct aaaacttcca cgtcattcaa tttcttaatg
     6241 acttcggtaa tcgtcacatt tcgttgttgc tcatctgttc ctagtttttt tgattggaga
     6301 tccacatcat caaataaccc attaaattga ttgtaggtag atgccaattg cacaaaggct
     6361 ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa gacgttgaaa
     6421 agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat cagatcttct
     6481 tttgattctt catctgaaag taattcgcga tacaacatcg tttgtttgct taccgtatca
     6541 tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg gtcggataaa
     6601 tatttataga aaatcagtcc caataaataa tttttatatt ctgacgcatc cattttgcta
     6661 cggagattat ctgccgcact aaataatttt tgatttaatt ctgcacccaa aaagatacac
     6721 tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc ccttttagaa
     6781 aaagcaaaaa aaaaaagaaa gaaacttatt cttgctaata aactacgcga aaggagtcta
//
LOCUS       DAFFSV010000134         6765 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFFSV010000134 DAFFSV010000000
VERSION     DAFFSV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.134"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            52..942
                     /locus_tag="LR110_002503"
     CDS             52..942
                     /locus_tag="LR110_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBL4823438.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1015..1839)
                     /gene="nadE"
                     /locus_tag="LR110_002504"
     CDS             complement(1015..1839)
                     /gene="nadE"
                     /locus_tag="LR110_002504"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBL4823439.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1851..3323)
                     /locus_tag="LR110_002505"
     CDS             complement(1851..3323)
                     /locus_tag="LR110_002505"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBL4823440.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3960..4322)
                     /locus_tag="LR110_002506"
     CDS             complement(3960..4322)
                     /locus_tag="LR110_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL4823441.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4545..5297
                     /locus_tag="LR110_002507"
     CDS             4545..5297
                     /locus_tag="LR110_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL4823442.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5437..6360)
                     /locus_tag="LR110_002508"
     CDS             complement(5437..6360)
                     /locus_tag="LR110_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4823443.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6375..6647)
                     /locus_tag="LR110_002509"
     CDS             complement(6375..6647)
                     /locus_tag="LR110_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823444.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2068 a         1350 c         1156 g         2191 t
ORIGIN      
        1 tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata tatgcaacta
       61 caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac aaggatcaag
      121 atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa agaacttgga
      181 gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa catcatcaaa
      241 acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat cgatattagt
      301 tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc tattggtcac
      361 tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga ttttatcggg
      421 aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg cattctctgg
      481 tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga ggatacattg
      541 gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa tgacaattgg
      601 ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac gagccctgga
      661 gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa tcttaatcaa
      721 tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa tggagaaccc
      781 ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca tttgcgaatc
      841 gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa aggattcggt
      901 aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc ttttctattt
      961 tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt attgttattt
     1021 ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct tgttccacca
     1081 tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat aatcatcaat
     1141 ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc catcttcaag
     1201 atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt gaagcaattg
     1261 tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac catcaccaaa
     1321 tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata cggctcctgc
     1381 tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga tatttccttt
     1441 gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg catcaacagc
     1501 tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag cagcttttgc
     1561 atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt gatagttttc
     1621 atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc ctgctaaagt
     1681 agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat attttttcaa
     1741 atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa ttgttggttt
     1801 gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc ctatgcttct
     1861 gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt atggttccag
     1921 cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt atactcttcc
     1981 catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc aggtaattcg
     2041 taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt aaagtctcga
     2101 acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc ttcttctcga
     2161 gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa atttcgagtg
     2221 atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct tgataacttg
     2281 atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa tactgcacca
     2341 agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac atcaatttta
     2401 gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga agcatagatt
     2461 tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga tatataggcc
     2521 atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa ttctttcgcc
     2581 acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac taagaacacg
     2641 cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc ataagattgg
     2701 actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc agcacgtaca
     2761 ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac tgctgcatcc
     2821 agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat gacagatttg
     2881 atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa cagtgctgtt
     2941 tctactaatt ggcagtgcgc taaaggccct tccacttgga tcaatggttc atttgcaaaa
     3001 actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt ttgtaaatac
     3061 tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc ggaatcattg
     3121 aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac cgcgtatccg
     3181 tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc agctcgacct
     3241 aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag cgtcaaactg
     3301 tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt catttcctac
     3361 ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg aaaaagcaca
     3421 tgttttacgg atatttcttg ctaaaaatga actccatctg ctaaaactat cctttcttat
     3481 tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg agacacgaag
     3541 aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct actacaatcg
     3601 tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca acataatcaa
     3661 tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat ccccaaagaa
     3721 acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga caagatatac
     3781 cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat acaaagtaag
     3841 aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt ctcgccttgt
     3901 tttttattgt atacattaga tatggagcag gttaccataa agatgattat tagtcgttat
     3961 taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt tggacgtttg
     4021 aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt gacatcttct
     4081 cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt gatttttttc
     4141 ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag cagttgtgtt
     4201 cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa gaattccact
     4261 actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc gattagcttc
     4321 atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat gttaccatct
     4381 ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga tttacctttt
     4441 atcacttaat agtataacat aatttcatga gaaaaaaata caattttttc ttgaccatct
     4501 cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag tacacaataa
     4561 agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat gatgagattg
     4621 gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc aaaaaagaag
     4681 tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg gatgatatcc
     4741 aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag catcagcaaa
     4801 tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa cagacacttg
     4861 atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg tttaaacaac
     4921 aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa tacggaaaaa
     4981 aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg tatgaagaaa
     5041 tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa gaaccagaag
     5101 atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttatct tacagctggc
     5161 ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc gatgaacggt
     5221 tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta aatgaaatta
     5281 tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta aaatgaccta
     5341 ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg tctgctccat
     5401 tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa acgtataagg
     5461 tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt ctccgcaagc
     5521 ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca aattatcgcg
     5581 tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg ctgcaaccat
     5641 atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc ctgcttgttt
     5701 tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag atggaaaaag
     5761 ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac ttcgccccaa
     5821 cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt ctgtaattga
     5881 ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt cacaaaaaac
     5941 tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac gagacgtaat
     6001 gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct ctttttcttt
     6061 ctgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg cttcaatgat
     6121 cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca ctccaatgct
     6181 tttcgttttt ccataactta actgtctggc atttgcatta gggatataat cgacttcttt
     6241 catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc cattgattac
     6301 acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa tgtttgccac
     6361 tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac agctggaccg
     6421 cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg cccgccccaa
     6481 tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac tatattccta
     6541 ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac aaacgcataa
     6601 aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc aagccctcct
     6661 tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt gttttgccaa
     6721 agaaaagttt tattcctctt tcattattac caaagtctct tattt
//
LOCUS       DAFFSV010000135         6643 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFFSV010000135 DAFFSV010000000
VERSION     DAFFSV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.135"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(1..859)
                     /locus_tag="LR110_002510"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="LR110_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="LR110_002511"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="LR110_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBL4823445.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="LR110_002512"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="LR110_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="LR110_002513"
     CDS             complement(2603..3421)
                     /locus_tag="LR110_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL4823446.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="LR110_002514"
     CDS             3582..4271
                     /locus_tag="LR110_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL4823447.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="LR110_002515"
     CDS             4285..5787
                     /locus_tag="LR110_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL4823448.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="LR110_002516"
     CDS             5800..6276
                     /locus_tag="LR110_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL4823449.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="LR110_002517"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="LR110_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAFFSV010000136         6576 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFFSV010000136 DAFFSV010000000
VERSION     DAFFSV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.136"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..561)
                     /locus_tag="LR110_002518"
     CDS             complement(<1..561)
                     /locus_tag="LR110_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBL4823450.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="LR110_002519"
     CDS             complement(748..1398)
                     /locus_tag="LR110_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823451.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="LR110_002520"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="LR110_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBL4823452.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /locus_tag="LR110_002521"
     CDS             complement(2910..3908)
                     /locus_tag="LR110_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBL4823453.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="LR110_002522"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="LR110_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBL4823454.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="LR110_002523"
     CDS             complement(4924..5655)
                     /locus_tag="LR110_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBL4823455.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..>6576)
                     /locus_tag="LR110_002524"
     CDS             complement(5659..>6576)
                     /locus_tag="LR110_002524"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBL4823456.1"
                     /translation="YSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
BASE COUNT         1970 a         1405 c         1023 g         2178 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaata
//
LOCUS       DAFFSV010000137         6463 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFFSV010000137 DAFFSV010000000
VERSION     DAFFSV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.137"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(105..560)
                     /locus_tag="LR110_002525"
     CDS             complement(105..560)
                     /locus_tag="LR110_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823457.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="LR110_002526"
     CDS             complement(714..1922)
                     /locus_tag="LR110_002526"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBL4823458.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="LR110_002527"
     CDS             complement(2034..2366)
                     /locus_tag="LR110_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL4823459.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="LR110_002528"
     CDS             2514..3389
                     /locus_tag="LR110_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL4823460.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="LR110_002529"
     CDS             complement(3471..4052)
                     /locus_tag="LR110_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBL4823461.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="LR110_002530"
     CDS             complement(4058..5317)
                     /locus_tag="LR110_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL4823462.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="LR110_002531"
     CDS             complement(5319..6365)
                     /locus_tag="LR110_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823463.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1801 a         1337 c         1102 g         2223 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata aaa
//
LOCUS       DAFFSV010000138         6377 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFFSV010000138 DAFFSV010000000
VERSION     DAFFSV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.138"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            1..816
                     /locus_tag="LR110_002532"
                     /pseudo
     CDS             1..816
                     /locus_tag="LR110_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            832..981
                     /locus_tag="LR110_002533"
     CDS             832..981
                     /locus_tag="LR110_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823464.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            1062..1658
                     /locus_tag="LR110_002534"
     CDS             1062..1658
                     /locus_tag="LR110_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL4823465.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1671..2570
                     /locus_tag="LR110_002535"
     CDS             1671..2570
                     /locus_tag="LR110_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL4823466.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            2573..2704
                     /gene="ssb"
                     /locus_tag="LR110_002536"
     CDS             2573..2704
                     /gene="ssb"
                     /locus_tag="LR110_002536"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL4823467.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            2726..3058
                     /gene="ssb"
                     /locus_tag="LR110_002537"
     CDS             2726..3058
                     /gene="ssb"
                     /locus_tag="LR110_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL4823468.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            3103..3336
                     /locus_tag="LR110_002538"
     CDS             3103..3336
                     /locus_tag="LR110_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823469.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(3495..3617)
                     /locus_tag="LR110_002539"
     CDS             complement(3495..3617)
                     /locus_tag="LR110_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBL4823470.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3807..4031
                     /locus_tag="LR110_002540"
     CDS             3807..4031
                     /locus_tag="LR110_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823471.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            4474..4680
                     /locus_tag="LR110_002541"
     CDS             4474..4680
                     /locus_tag="LR110_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL4823472.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4680..4931
                     /locus_tag="LR110_002542"
     CDS             4680..4931
                     /locus_tag="LR110_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823473.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            4947..5102
                     /locus_tag="LR110_002543"
     CDS             4947..5102
                     /locus_tag="LR110_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823474.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIE"
     gene            5112..5384
                     /locus_tag="LR110_002544"
     CDS             5112..5384
                     /locus_tag="LR110_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823475.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            5829..6008
                     /locus_tag="LR110_002545"
     CDS             5829..6008
                     /locus_tag="LR110_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823476.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
BASE COUNT         2290 a         1072 c         1308 g         1707 t
ORIGIN      
        1 taaaggcaat cgaaaaggtg ccgctagatc taggttggaa gaagttattt ggaaaagaga
       61 gcgacggaaa agagaaggaa gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg
      121 tgacggtgga tctacaagtt cttgaaaaag aaaccaagcc accacaacct tataccgaag
      181 gtacgttgat cactgcaatg aaaacagctg ggaaaaccgt ggatagtgaa gaagcgcaat
      241 ccatattgaa agaagtggaa ggaatcggta cagaagcgac acgggcgaat attattgaaa
      301 ccttgaaaca aaaggaatat atcaaggtag agaaaaataa gctcgtggtg acgaataaag
      361 ggattttgct ttgccaagcc gtggaaaaag agccgctgct tacgagcgcc gaaatgaccg
      421 ccaaatggga aagctacttg ttaaagatcg gtgaacgaaa aggaacacaa accacttttc
      481 tagccaatat ccaaaaattt gtctctcact tattggaagt cgtaccaggg caaatccaat
      541 ccactgattt tggatcgacg ttacaagaag tgaaagcagc cagtgaaaag caagaagccg
      601 cgcgtcattt aggaatttgt ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg
      661 tggcagcttg tacgtctgaa gcctgtgatt ttaaaaggtc agaagggttc atttgaagca
      721 acgattgttt taaaagaaga cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg
      781 aacttcaaga aaagaacacg ccgcactaca aaataatgga gggttaatct tatgcgtaat
      841 accacacaca aacaacaaga aatcgaaaat caaaaagcca aaattcgttc agaacaggaa
      901 aaaatgcgtg ccagcaaacg aagactgcag caattgaaga accagcagag gattcaaaaa
      961 gaaatcgaat tagatcgata aaatcttcct aactattgta tacgtataaa cttatgttat
     1021 actgttttta taagtttata cgtatacaaa ggagggagaa aatgacaaaa gcaatggtat
     1081 acaagctgct agaagaattt aatggaactt tggctttgaa ggatgccaaa aaagcgggca
     1141 tttctcctgt aacgatcaaa cgtatggtag atcgagggga gctggatcga gagtatccag
     1201 gttttttcac cttacctggt caatttcctg atgaattgtt tatggcgcaa aaaaaatatg
     1261 aacgcggcat tatttcacat attaccgcgt tagatttgta tgatttaaca gatatgatcc
     1321 ctagacagat cgatctgact gtaccttacg gttatcacgt ttctgaaaaa ggattgaagg
     1381 aattcgcagt tgagctacac tatacaaaag cggaatggta tgaattaggt aagattgaaa
     1441 taaaaagtcg ctatgggaat cctattatag cttatgatcc agaaaaaacc ttgtgtgaca
     1501 tttggaatcc ctggtataac gttgaggatg aaatcaaagt aaaagcaatc aaaaactaca
     1561 tggagtcgga cagaaaaaat cttagaaaat tgaatgagta tcgacgaatt ttgcctacag
     1621 ataaaacgat gcgctcttat attatggctt taaattaaag gagaatacac atgacaccta
     1681 cgcaattaaa agcaaaagtg aagaatattt ctagagaaaa agaagttgat ccgcagcttg
     1741 ttttaagaca ttttatgatg gaaaaattct tggaaaaaat ctcagagtct ccttatcgtg
     1801 aaaattttgt gttaaaaggc ggcttcttaa ttggctcaaa gtatggcatt gaaaatcgaa
     1861 caactaaaga cattgatacg actttacggg aaatgaaagt aaccaaagaa acattgacaa
     1921 cggttctaaa cgatattttt tcaacgccga cgaaagaagg cattcaattt gagattcaag
     1981 gaatgaaaga aacgagggaa gctgattatt atccagggtt tagcttacgt gtactggcac
     2041 atttggaaaa catgcggcca gattttaagg tagatgttac tacaggtgac tcgatctatc
     2101 ctgcgacgat cactcacagt cataaattga tgtttgagga tcgaacgatt cctttggaat
     2161 cctatcctac agaacaaatt attgctgaaa aattaagtgc aacctttgat tttttaacag
     2221 ataattcgag aggaaaagat ttttatgatc tctatactat tcctaaaatg gagaagatcg
     2281 atgagaagac cttgaaagat agtgtgagaa atacatttac acgtcgagga aaaactgatc
     2341 cgttaaagat atactatgaa aaggatatgg atgtactgaa aaatagtcac gagctaaaag
     2401 atacctggaa gaaatatcaa aaaagcaatc cttatgctgc gtcgatcacg tacgatgaga
     2461 caatggattc gattagtaat ttgatggaaa aagtgattca cgtcgaagaa aaagagcgaa
     2521 aactagctta tcaaagacac caacagatga atagagaaat ggagagataa agatgattaa
     2581 taatgtgaca ttagtaggac gcttaaccaa agatcctgat ttacgttata ccgcaagtgg
     2641 tactgcagta gcgactttta ccctagccgt caaccgaaat ttcacgaatc aaaacggcaa
     2701 ttgagaagcc gattttatta actgcgtgat ttggcgcaaa ccagcagaaa caatggcgac
     2761 attagctaaa aaaggtagct tgattggtgt agttgggcgg attcaaacgc ggacttacga
     2821 caaccagcaa ggacaacgtg tatatgtgac tgaagttgta gcagataact ttcaactatt
     2881 agaaagtaaa gcagcgacag agagccgtgc gcacgctgat caatcgtcca ccagtccaag
     2941 caccgccacc tttgaacaac ggaatacagc gacgcctaac aacaatggat tgaacgcttc
     3001 acagaatcca tttggtggac aatcaatcga tattagcgat gatgatctac cgttttagtt
     3061 tgagaaagaa ggagtaagca tggcaaaaaa attagtctga cgatggatct attgataccc
     3121 gtaattgcat ttatagacgg gatttctttg ggcgtcactg aaaatccttt ggtaaataga
     3181 attgcgaaag gatcactatt tcttgtattc gtgtatcttg tctgttctta tcttttaaaa
     3241 agagcaggaa aggataattc cttttggcga ttgactaaag tcacagtagc agcgacagct
     3301 gttatactta cggctattct accgctttat atgtagagtt aacgggaaat cgttgaactt
     3361 cttgcgaaat attgctataa tgaacatgaa aaagaggagt tgcgctaaca acccctcatt
     3421 aacagcaccg attaaagaac ggtggcagaa tattagttat ttggaaataa ccgctgacct
     3481 gctaaagtag acggttattt ctttttgtca tttttaagca tatttacaat caaactaatc
     3541 aaagcaatgg taaacatacc aaaaccaaga atggtttgaa ttgtctcata tgcggacaaa
     3601 aggcttactc ctttcataga ttttagcact tacattcata agcaccacca cctttccaga
     3661 agtcgccacc gtcttcaaac tttactgtct tgactattat agcatatcta aaatcgaata
     3721 aaaattctca attttagctg tttttgctat aattttagcg aggtgatcgt gatggaacaa
     3781 tatattggtt tactcgataa attagggtga tcaagacaca gccattgctt gtgcgattta
     3841 cattacgcac gagtcatgaa ccaatcaatt gtgtagtggc caatatagaa atagcggata
     3901 aattgctgat tttggaagaa ggcaagtata ctatttcagt gacaggtcac ttcaataaac
     3961 gaaagcaact agtcattgaa tcaatgcaaa ttagaaaccc tgatcatttt acgcgatcga
     4021 tgagcatata aaagaagagc tgcagtccta aatgaggatt gtagctcttt ttgttttaca
     4081 tagaaacagt aaaaatgaaa aggcctgttt aggaaggcac aagcgccttc tcaattgaaa
     4141 gacctttctc tcactccaac cgccaccgcc gttcgagaag aaaggaagtg aacgctacgc
     4201 gttcactaag ataaagggtc ttgcatagca agacaaaata gaaaaaattt cctctcacca
     4261 aaaggttcgc tcctgttcgt tatctcttta ttgatttgtc agaccggcaa caagttgcct
     4321 tttatcacca aaaagcgaca aggacaaggg taaatggcac gctgcgcgcc cttgtcttgt
     4381 cgcttttcgt tgattcattc tggccaacaa atcaataaag aaagaactaa cgaaaggaag
     4441 taaaaccttt cctaagagga ggcagaatca atcatgcgaa agaaatttga ttattggggt
     4501 gttcccttca gcgaattatt cccaaactat catgcaccac atactgtcga atgcgattgt
     4561 ggagaaagag cgaaatgtat caaaagctat tgtttgtatc aatgtccaac atgcgggaaa
     4621 aaatatacct tgtcttatgg cgattatatt ttaattgatg agaaaggaaa gaaacgctga
     4681 tgaaaacaca acacaaatta ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag
     4741 aagtcatttc tgaaaaagta gcagaagttt tacttattcc gcggtactgg acgatagaaa
     4801 cccctttagt agaggatgca ccgactatgc tatttgaaga cctagtgaag attgcaggat
     4861 atgaactgca ttatcaagaa aacttggaaa cttatgaaaa cgaatattgg ttaaccaaat
     4921 tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta ctatgacgaa tttgtcaaat
     4981 tacctttgga taaaatggca caaaaaatgg aggatatgac gttcctatac aacgaaacac
     5041 gtgttccaaa aaaacattat aaagaaaaat tgtcggtagc tgtggaagaa atgattgaat
     5101 aaggtgtcga aatgaatctt attgcgacgt actatcgaac actggaagaa ctgaaaaagc
     5161 aaaatgcaaa atggtttttc caagccttac tatgcttgga agtaggtgtc aaaccaagca
     5221 ctattaaacc ttctgaatac caagctttag aactaacgta tgcgaaattt atcgaaacga
     5281 aaaaagccaa gactgtttca agtgaatggt tagattattt tgaaaatatc aataagtatg
     5341 gcgcatatta caccatgaaa aaggaagaca acgaaaatga ataaggtgat ttatcaacta
     5401 acaacggatt ccatatcaac gatttgaaag aaaaaaaggt caataatcat ttataccgaa
     5461 aagtgagaat gtgtgaaaca aggacaaaaa aagcctagta actaaggaaa tggttcttat
     5521 ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa acgggcaaag
     5581 cccgataact taagagaaag aaggtgatcc atttgaacaa gattattccc ttattgttag
     5641 tactgtttgt tggcgaaata cttatgcgag atagtgcagc tgtacagtta aacctcgctg
     5701 tatttttggt aatactggtg ttttgtttga tcttttggcg gtttgtatta gtcctcgtga
     5761 gtctactatt caagatgatt gcagccatat ttttcatagg gttatttcga tgaaaggagt
     5821 gagtaccaat gccaattgca ataggaaata aacggttacc cgtgacatta gatgaaaaga
     5881 gacaaaaaga attgcagcaa ctaaagcaga agtatggcaa aagtgaatct aagattatgt
     5941 gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatgta ccagcgctaa
     6001 aaaagtgacg tcacttttta atttgatcta tataagtgat atcactttta ccaaggaggg
     6061 aattaaatgt taaaaactaa aaagaattcg tacaaattaa agcttagtat actatctatt
     6121 atttgtcttt taggcatgat aattagtggt catttgtata gagtggcata tacgcagttt
     6181 attgagtaca aggttcactc tttaggatta gctggggatc gatacgttga atatgtccat
     6241 tattcattaa aatgttcttc atatattctt ctttggctta ttttttcatt gtttgaattg
     6301 ctaaatttaa tagcaatatc ttcatatcac ttagataagc taaataccaa atctagccgt
     6361 tataacgaag tgtttaa
//
LOCUS       DAFFSV010000139         6211 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFFSV010000139 DAFFSV010000000
VERSION     DAFFSV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.139"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            160..1317
                     /locus_tag="LR110_002546"
     CDS             160..1317
                     /locus_tag="LR110_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823477.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1343..1783)
                     /locus_tag="LR110_002547"
     CDS             complement(1343..1783)
                     /locus_tag="LR110_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL4823478.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1943..2449
                     /locus_tag="LR110_002548"
     CDS             1943..2449
                     /locus_tag="LR110_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL4823479.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2617..4530
                     /locus_tag="LR110_002549"
     CDS             2617..4530
                     /locus_tag="LR110_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL4823480.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4535..5536
                     /locus_tag="LR110_002550"
     CDS             4535..5536
                     /locus_tag="LR110_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL4823481.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5738..5944)
                     /locus_tag="LR110_002551"
     CDS             complement(5738..5944)
                     /locus_tag="LR110_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBL4823482.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2292 a          945 c         1231 g         1743 t
ORIGIN      
        1 agaaaacaag atgataaaag tcgagttttc ttctcagttt ttactgagta aagtttattc
       61 tatactttta gagggaacga ttggttatat tttactggat tcactttaca aaggtactta
      121 tcaatcctta gaaaatctta gtcaaaaaat tatataagtt tgcgtacgat tcaaagaaaa
      181 ttaagagaac ttaatactat tttgaaaaac tatcatttat cttgcagctt gaaaaaaggt
      241 aatccgatag aaggagaaga ataccgtatc cgatactttt ttcacttgat gtattggcag
      301 tcattcgacg aaaatcattc tgatatatta tcaagtataa aaaataaacg tcaactatta
      361 gaaaaatttg cgaaacatgc gccatatacg cgtagtatag atagaaagaa atggatcagc
      421 ctactaagaa tttcgttgca acgaatgaaa caaggccact cgattacgta tgtcccagaa
      481 gagatgaaca aatttgtgca tccattgata acaaaagaag agtttacgca ttacatacta
      541 gagccttttt ttgaagcaaa cttctggtcg atgaatcaca tcactccaac agagcaagct
      601 tacctttatt ttatgtttgg tgtaatgaac acctatttgg aagaagattt gttggatact
      661 ccagataaag tacaagaaag ttggggctgg attgaatcgt ctattgtaga aaattttgaa
      721 aaaaacttca agatttgttt tacaacttca gaaaaaaatt atctatatgt caatttggca
      781 atgatccatt tgtattcaag ggtttttggg acaaaaaaga aggtggatgc ttttggaaaa
      841 tcagcagaag acagagattt tcagcatata tttccagtga tgtatccaat catgcaacag
      901 ttttttagtg gggtagctcg cagaattcca gagcttaata cttattatga aaagaacaga
      961 cggttgattt ttcaatactg tatgttagtc agggatattt ttataaaaca cgaacaacct
     1021 gtcatattct atattcaatc aaagtatggt aaaacacagg aattatgggc aaagaaaagg
     1081 atattatcta tgactgaacg cccaataaaa tatagtgcat ccgctgatca gttacctgat
     1141 attgtcatta gcgattatcc tatcgacaaa aaggcatatg atcaaacgaa tacacagata
     1201 ttttactgga atggacaacc tacaaaaaat gattgggcgc gtctgaaagc tgaaatcata
     1261 aaaatcagga atgagaaaaa tagatccata ttggaaaaat tggatttcgt taattaagaa
     1321 aagcagattg tttatgccaa aattaagaca ttgcaaaaag attattttct ttaaaatcga
     1381 caagatgacc agaaagcaag agttcgttga gctcttgctt tcttttagtt ttagaaagga
     1441 acatcctgat tttatcttcg ctaaatccag tcaaaagttt tgcagattgg tcgtagataa
     1501 tcggtttttt caataaagaa ggggatgtac gaatctcaat atatagttca ctcaaactga
     1561 gttcatctat tctgtgaaca agatttttat aactaataga atgagtagag agcatttcgg
     1621 tcacgccgtt gtttgtcaat tttagtaagt ggataaattc atttttggtt agtggttgac
     1681 gaattaagtt ctgttcgata aacggtatat catggacgat aagccaacgt tttgcgcgtc
     1741 ttgaagacga gcagccagaa cctgtataca atttaatcat catacattcc tccaatagct
     1801 aagtatttat gaaataataa catgaagaaa tagtcgaaaa gtgccattta gaggtgtaaa
     1861 ataagaaaat gttgtcacgt ataatggaac tagctagata agaaatacta ttaaatcaag
     1921 aaaaaagaaa gaaggcacaa gtatgtcaaa cacagccgac gcaatagtag tgatcgattt
     1981 acaaaaaggg gtatgttttg attcttcgcc gatacacgaa ttagaaacag tgacagcatt
     2041 agtgaataaa agaatcaaaa catacgaaac gattgggaaa ccagttattt tcgttcaaca
     2101 cgaagatgct caattagtgc cacaaacaaa accatgggag ctattgtcgg aattatctat
     2161 tcctgagcac agttattttg tgagaaaaac ccatgcgaat tcattttata agactaattt
     2221 gagtgtttta ttaactgaat tggactgtca ttcgatcgaa ttatgtgggg cacaagcaga
     2281 gtattgtata gatacgactg tgaagtttgc tcatggatta ggataccagc ttatgatgtg
     2341 tagaggagca acgacaactt atgataatcc ttttatgaaa gcaaaggaaa caatcgcttt
     2401 ttacgagaaa atttgggata aacgatttct gacatttttg gagcaataag ttgtttcttc
     2461 gtttaaaaaa agcagaatgg atactggttt atccattctg ctttattacg taaaatgaga
     2521 aaataatcct gttctatttt tatgggataa accatttatt ttttgtataa aaaatgtttg
     2581 cgtttacata taattctcct agaaacataa aaaggagtga atagcttgag aacaaaacaa
     2641 gtattaaagg gagcgttggt catcttattt gctgtaagta caagcgtccc gcaatggaca
     2701 gtatatggag agagcatttc ttccgaatcg gaatctcggg aggtagaaac cacagaaact
     2761 actgatgctg gaaatgaaac gaaatcgagt acttcacaaa cagaaacaaa tactgaaaca
     2821 acggttgaga catctgttac tagcacagat caagaaacga ccgctaccac agataaccaa
     2881 caaacagaag aagcaacagc tacaaaaatg tcaaacgacg aatcctcttc tgctaaagta
     2941 agtgacaaag agaatgggac agaaaatgat gatatcggta aaggtgaaaa tttcaatggt
     3001 aaagaatatt tgaattactt cggtaaacag atgacagaaa tacttaatgg accaacagaa
     3061 gacatcaagt caaaattaat agagttatta gtcactgagc ttgctgttaa tgattatggt
     3121 gtagataaag agattatcaa acatctgatt gctacttatc tattgtacga tatcgctttt
     3181 gcaaaaaaca gtgtgttgat gtcgatgctt ggaggtacag atgatgggac aacgcctaca
     3241 gaaaacacag gcttctatcc aaatacaaaa caacttcatc aaactgtact aaacaaacaa
     3301 gatggcaaaa tgatggatgt atatgcatat tatgtcgatc aaggttcaga taaaacagtc
     3361 atgatccatg gcggatttcg agggaattgg aataacggga tcgttacaga agaatacaat
     3421 gatttttata aggccgggta taatttatta tttgttgatt ccagagcgac tggtaatagc
     3481 ggaggcgatt atgtgaccta tggacaatat gagtcagatg acgtccttta ctggataaat
     3541 caagaagttc gagagcggcc atctcaaaag attttacttt atggtggttc gatgggagca
     3601 gctacgatga tgtctgtatt agcgaaagat attcctgtca atgtcaaagg aattattgaa
     3661 aattgcggat ttgcatcaat tgatgaacaa ctacgtttta cgtacagcca gacagtagcc
     3721 cccgcactac cggatgccat aaaaaatcag ttagacatca ttggggatca agagcatgaa
     3781 gatttattta tgggcctttt gaaacaatac tattttgatc aggagatgca tttggataca
     3841 aaagctgctc tacctacgat tggaatgtca gattcattgc ctaaattgat tatccatgga
     3901 acagcagatg atgtagtacc tgtgtctaac gcgcaaaaat tatatgagtt atcaggaggg
     3961 tataaagatc ttttattagt agaaggagca gggcatggaa aggcacagga agttgaccat
     4021 gctgcctaca cgaaacatgt aacggacttt ttgaaagtag tgttccatga tcagataaac
     4081 gtaaagtatg tggatgaaaa caataaaagt ctactcaaag accaggacga aatccagctt
     4141 tacggagctt atggagaaaa ttatatgaca gagcaaaaga cattcgaagg atacgaatta
     4201 gcaaatgttg aaggaccgac tgaagggatt tttaatgaga cgataccaac tattattttc
     4261 tattacaaaa aaatcccagt agctccacca aaaaagcaag atccgacaga gaatgcagac
     4321 aataagggaa agaatgaacc tcgagaggat caatcaagtg atgatacgca accgattcag
     4381 atgacagtga aagtacaaaa aaagacagat gcaaaaaaaa gaatgttgcc gaaaactgga
     4441 gaaaagaaac attcgcttct catttattca ggtggatttt tatctgttag cacgattatc
     4501 acatggattt ggcgaagaaa gagagggtaa atgaatgaaa tggttcaagc gaatagtgat
     4561 tgcagtacca gttatcatcg tagttatttt agtcactgta tttttgatta atcaaatcac
     4621 accaaagccg attagcttgc ttgttagaaa acaatttgac acaagtgaaa aagaacaagt
     4681 atatgcccga ccagatgatt tccagcaaaa aatcgaacaa atcgatataa aaaaagaaat
     4741 atcctatcct tcaaagtaca aaaataataa tatggatatc tacacaccga aagtggaaaa
     4801 gaaaaaatta ccgattcttt tctggatgca tggaggcgca tatgtaggag gggataaaaa
     4861 tgattgtcgt gattatttgg aatatctatg ttcagacacg cagcaaatca tcgtaaatat
     4921 agattatgaa cgatcaccgg aagcaaaaca tcctacacca gttatccaat tgaacgaagc
     4981 cattcaagca gctaaaaaag agataaaaga tcaagcagat tggtcgaata tttcgattgg
     5041 tggaaattcg gcaggtgcac aaattagtgg agaatatttg ttagcacttc aaaatgaaga
     5101 aattaaaaaa gctgatcatc ttgatccgac tttggagaac aagcagatta cgaagtttat
     5161 ttctttgtct ggtctgttgg atccttctgc acttactcaa gtaagtgata aaatctctag
     5221 ctttctttat gaaaaatgtg gatgggctta ctttgataat aaggactttg aacaatcaga
     5281 gaaggtcaaa agtttagcct tggtcagaca tgcggatcgt tggacgcaaa acacattctt
     5341 aacggatgga aatacaaata cgtttacgaa acaaatggaa gaagtaactt ctgtatttga
     5401 gaaagcgggg gtgaaagtca caaaagtaga ttatgcggag aaaaatgctg ttttgaacca
     5461 tgaatatcag tttgatttca gcaataaaca agcgcaagaa acatatcaaa agttagtaat
     5521 gtttttcaag gagtaaagtc ttatgatgca aaagaggttg taatctgtca cttagctcca
     5581 agtcacaagg agcaatttca ataaacaagc ttttcatggt ttatgaatat tgtaacttgt
     5641 gactgaggag ttagagatgg caaggggcac cttttattcg aaatttggga atatcacacg
     5701 attattgtca cgatctcttt tacgtaaatt ttataagtca gatttgttgt tttgatcgtt
     5761 taaaaaaagc aacaaatccg aagcctgcgg aggcaagaag ggacaaaaag ccgagtggta
     5821 agcaaaagaa tgctggttcc actaatattc cgttgttatt gatataaggc tgggaacaaa
     5881 gatgaatagc aaataaaatc aatgcactag cgaaaaaaag caaagaaata tggatagctt
     5941 tcataagcag cacctccttt tttataattg tatgataaat agattaagaa tctataaatg
     6001 tgacaagttt gttaggatgt gaataaaatg atcatctagc gaacttgaaa caaagatggc
     6061 taatgataag caattaatga ttcagcaatc agataggtac aaagtttacc atcatacgtc
     6121 tagaccaata atagaaaata tgggaatata atagaaaaga tttaggatac ccccaaaaga
     6181 atttccccaa aaattcgatg tatcctaaac t
//
LOCUS       DAFFSV010000140         6154 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFFSV010000140 DAFFSV010000000
VERSION     DAFFSV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.140"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(89..1219)
                     /locus_tag="LR110_002552"
     CDS             complement(89..1219)
                     /locus_tag="LR110_002552"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBL4823483.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1279..1506)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1868..3070)
                     /locus_tag="LR110_002553"
     CDS             complement(1868..3070)
                     /locus_tag="LR110_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL4823484.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3130..3333)
                     /locus_tag="LR110_002554"
     CDS             complement(3130..3333)
                     /locus_tag="LR110_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBL4823485.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3475..4707)
                     /locus_tag="LR110_002555"
                     /pseudo
     CDS             complement(3475..4707)
                     /locus_tag="LR110_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4870..5682
                     /locus_tag="LR110_002556"
     CDS             4870..5682
                     /locus_tag="LR110_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823486.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1821 a         1267 c          984 g         2082 t
ORIGIN      
        1 tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg actttttggg
       61 acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg acttgctgca
      121 ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct tctgttgaag
      181 caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt tgtgcttctt
      241 ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa gtaactaatc
      301 ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa tctcctgctc
      361 ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac tcttctgcaa
      421 tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg cagatatgtg
      481 ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc tctgtacaaa
      541 tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt ttagtttgat
      601 cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct ttttcataaa
      661 aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca gatagatcag
      721 gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt cgtaaaatca
      781 tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc actacttctt
      841 tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat ttatctacta
      901 cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat ccagtttctg
      961 gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa aatccacgcc
     1021 gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc actaattttt
     1081 ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct tcttgaactt
     1141 tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga tctactgtaa
     1201 atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa aaaaaatcct
     1261 tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt ttttcagaaa
     1321 aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga aagactgttc
     1381 gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt caacttctag
     1441 ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc ttctcatcgg
     1501 atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag ggaataagaa
     1561 caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat aaagcttttc
     1621 taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa atgatcatac
     1681 ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa aactaagtcc
     1741 tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac atcttgaaca
     1801 ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg gtagaaccgt
     1861 ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc tcttgaacat
     1921 tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg taagttccta
     1981 aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt tgaatattct
     2041 gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc ggtttgacac
     2101 ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc gtaaatgttc
     2161 cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca taccactgtc
     2221 tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat ttagttttgg
     2281 gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca atcgttatcg
     2341 tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca atccgaatgc
     2401 cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc ttatcatcct
     2461 tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact ttcttattct
     2521 cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc tctttaacag
     2581 cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat tttgataacg
     2641 tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt tcaaataacc
     2701 aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa aactgcatac
     2761 gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt ctctcgtacc
     2821 attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta gaacacagtt
     2881 gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa gcaattttgg
     2941 catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca ttttcatcta
     3001 tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa atatttttaa
     3061 tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat agtaccgcgc
     3121 ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc gggatggcac
     3181 cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa gatatgcctt
     3241 gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta tcaaaattgc
     3301 acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt ctttactcct
     3361 tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt tatactcgtc
     3421 ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc ttttttagta
     3481 gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa cctcctcctg
     3541 ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg gattcatttc
     3601 tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga cctgtgcaat
     3661 aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct caaaactttg
     3721 aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg ctgcccattt
     3781 ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa ttgctccatt
     3841 aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct ttgccaagct
     3901 ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg cctcttctaa
     3961 aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa aacgacactg
     4021 actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta tgatatccgt
     4081 accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta ttaccttatg
     4141 cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat caatccgtgt
     4201 aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg tttttcgtgt
     4261 ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca cagttccttt
     4321 aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt attggccaat
     4381 atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt caataatctc
     4441 ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat caatcaaaac
     4501 acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca atttttcttc
     4561 acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa tccccacttt
     4621 ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa tggcttcacc
     4681 gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt acttgtcaaa
     4741 gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta ttttaagtca
     4801 atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt atcctgagga
     4861 ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca gattcgccaa
     4921 gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc gcgaagtaca
     4981 gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca ccaaatcgca
     5041 gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa tcaatacatt
     5101 ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg tctcatagta
     5161 gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat gattgaattt
     5221 tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca atgtatcgat
     5281 gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca gctctatttg
     5341 caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc caactttagt
     5401 gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt tgggaaacat
     5461 ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca attgaccagc
     5521 gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca gtacacagag
     5581 gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat ggaagtcgag
     5641 aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa aattgataag
     5701 cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca cagtttcctg
     5761 ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg ctacgccttc
     5821 ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg acctggagtt
     5881 tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc ataatcagcg
     5941 cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct ggttagttaa
     6001 tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc aacctttaaa
     6061 aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca ttctcaaatt
     6121 ctagttcttt aattgatttc agttgaggag cagt
//
LOCUS       DAFFSV010000141         5907 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFFSV010000141 DAFFSV010000000
VERSION     DAFFSV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.141"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(95..667)
                     /locus_tag="LR110_002557"
     CDS             complement(95..667)
                     /locus_tag="LR110_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL4823487.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="LR110_002558"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="LR110_002558"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL4823488.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="LR110_002559"
     CDS             complement(1352..1867)
                     /locus_tag="LR110_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBL4823489.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="LR110_002560"
     CDS             complement(1869..2153)
                     /locus_tag="LR110_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4823490.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="LR110_002561"
     CDS             complement(2187..3515)
                     /locus_tag="LR110_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL4823491.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="LR110_002562"
     CDS             complement(3529..3984)
                     /locus_tag="LR110_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4823492.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..>5907)
                     /locus_tag="LR110_002563"
     CDS             complement(3987..>5907)
                     /locus_tag="LR110_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4823493.1"
                     /translation="KLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLEDYVVNYQVTI
                     ERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHL
                     SQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYAN
                     DMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLL
                     KGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVT
                     NNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQN
                     PLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNII
                     IICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIP
                     LVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTG
                     VQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIE
                     SNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQV
                     HDSEKLLRIFQQKGE"
BASE COUNT         1919 a         1147 c          836 g         2005 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taatttt
//
LOCUS       DAFFSV010000142         5706 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFFSV010000142 DAFFSV010000000
VERSION     DAFFSV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.143"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..213)
                     /locus_tag="LR110_002564"
     CDS             complement(<1..213)
                     /locus_tag="LR110_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBL4823494.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYN"
     gene            complement(395..1051)
                     /locus_tag="LR110_002565"
     CDS             complement(395..1051)
                     /locus_tag="LR110_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL4823495.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1178..1651)
                     /gene="dfrF"
                     /locus_tag="LR110_002566"
     CDS             complement(1178..1651)
                     /gene="dfrF"
                     /locus_tag="LR110_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL4823496.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2070..2288)
                     /locus_tag="LR110_002567"
                     /pseudo
     CDS             complement(2070..2288)
                     /locus_tag="LR110_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2319..3533)
                     /locus_tag="LR110_002568"
     CDS             complement(2319..3533)
                     /locus_tag="LR110_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL4823497.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAERLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(3874..4116)
                     /locus_tag="LR110_002569"
     CDS             complement(3874..4116)
                     /locus_tag="LR110_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL4823498.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4148..4705)
                     /locus_tag="LR110_002570"
     CDS             complement(4148..4705)
                     /locus_tag="LR110_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL4823499.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(4718..4906)
                     /locus_tag="LR110_002571"
     CDS             complement(4718..4906)
                     /locus_tag="LR110_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL4823500.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(4919..5485)
                     /gene="amaP"
                     /locus_tag="LR110_002572"
     CDS             complement(4919..5485)
                     /gene="amaP"
                     /locus_tag="LR110_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL4823501.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1708 a         1202 c          842 g         1954 t
ORIGIN      
        1 attatatacc cattcttgcg gagaaagttc agtaacaacc tgatgagaag tattccaccc
       61 cattaagaca gtatctatat ctttggtaaa ttctgtataa gtgtcgatgt tttcattgtc
      121 atttccatga ccatttagcc aagctactcc accattaccg tccgcaatat atccgtcaag
      181 gctcatggca ataaataaaa ctacttttct cattttttct cttttataaa tacaaacagg
      241 aatttatttc tcttttttac tcataaggga acaactgatt gctacaaata aacccattaa
      301 aatccatgga aatgctgctc ttataaaatc actaaccata ctttattcct ccttaaattc
      361 caatttcctt agttctacaa actgaaattt ttgcctatga atgtatcttg ttctttatac
      421 gcaaacaggc ttctgtgtgc ataatccaat gtcttgaaaa cggcatttga attagtccac
      481 aaatatcttt attacaccaa taatcaatca gccaaattgc tttttcattg tcgtttacta
      541 cattcaacga ttctaagtat ccttttcttt ctttcggtat ttttcttttc aattcatcat
      601 aagataaccg ttcaagcatt ttctcagtgc tttcccatac ttcgaaaata tataaatata
      661 attcttccag attaagctgt tttgaaaagt ctgcaatctg ctgtttcatg agttcatttc
      721 ctgttgtaat gataggtgaa ttgatacgct cttgataatt gcctgcaaaa aatacttgtt
      781 catcttcatt tatcactgta tgcacaacta tatcttcaat gcggaaaata tgccaaatag
      841 aataagcaat cgtcttacta tgataaccat cagcgtttat gaaggggatt gagttaaaat
      901 cttctctgca taattcttct ttgaaagata ctaaggtttg tattaactga cttcgtaaag
      961 taagtaaagt atcaataccc ctcttataag tatctttctt ttttatttgt gcctgcattg
     1021 ttttattaag ttcagaccat tccttattca tattcaatcc tctctcaaat tctgatttta
     1081 tcgccccata caaactggaa tttgtcttac aacaatttat atatgcataa aaagcaatat
     1141 atttatgaaa acaccctaaa tccaaaattt actcaattca ttttttcctt acataaaatg
     1201 ttctcgtata tttcacttcc tcaccaagtg tttcccctat caaaacttca aaatcattga
     1261 tatcaaattc tggaaagaat gtatctccat cttcaatatt taaatctact tctgtgatat
     1321 acattttatc tactatttgc aaagcttcct taaatagtcc atatccacca gatatgtata
     1381 catctcgtcc tttagccaat aataatgcat cttctaatga tttaactgaa actaaattat
     1441 ctccttgata ctctgttgtg gtggaaacaa caatattcat tctattaggc aacggatgac
     1501 cgatttcttc ataagacttt cgccccataa taaccacatt acccgttgtt aactctctaa
     1561 attgcttttg ttctcccttt attttccatg gtatattacc attcttgcct ataacattat
     1621 tctttgacct cgcaacaatc aaacctatca tctatttcat cctccataat atgatgtcgt
     1681 acctgtgatt ccatcaagtg caccacatct taacaaactc aaaattatct cgaactaacc
     1741 agctcgacaa attccttttt tgtcttcatc tgcattatat catattgtta taagcattgc
     1801 catttctttt ctgcttttga aaagttactt atcagcctat gttttgtaaa gggtgatatt
     1861 cagcatttct cacgcaacaa gcccttgaca aaccatagac taataagttt taaggtaatc
     1921 aagcagaaat gaaatagtct gctgtttcac ggcaacaaaa aagagatacc aacacactta
     1981 tgtagtatct cttagtctag tagcaatatt cttttgtttg caagcaggta aacatgggct
     2041 gtttcttaat atctttttgt ttgggaaaca cctttttccc atcctttcac cgttgccatc
     2101 tctgctgcgc acaatgccac agggataaac catagcaaac cgcctaataa caagaaaaat
     2161 actaagctga agcctgaggt cgcgaatgtg ggatattcat ataatgaaac gaccattgag
     2221 atggtcatag ccaaaaagcc aaagaacgtt aatttttttg caggaatttt cgttttgctt
     2281 tgttgcatga atcttcctct tttctatttg aatttgtttc aattgagttt gttgccaaag
     2341 aagaaaaagt ccaatccttc ttcttgttct tcttttgtag tagtgatcgt tgtaaagtgc
     2401 atcgccgatt ttaaaatcac tgttgtcagc acataggcat acacggcagt aatgatgaca
     2461 ccaatcactt gaatccctaa gaagtaccag ctgcttggat tcgttagtaa atggctgtta
     2521 gatgcaaaga tccctatcag aatcgtacct gtgaatccgc ccatgccatg aacaccccac
     2581 acatcaagtg catcatccca ttgtttcttt ttacgaaaat caacagcagc atgacagacg
     2641 atacccgcaa tgatcccaat cagcatggca ctttttgctt ctacataacc ggcacaaggc
     2701 gtaatggctg ccaatccagc aacggatcct gttagtacgt cgacaaaatc aaagccattt
     2761 ccttttactt ttgcaatgat cagccaagta atcatcgcaa atgcaagagc gatgaatgtt
     2821 gaaccaaatg cattgactgc ttgttcatct gctcgaagtg caccaccgga attaaaaccg
     2881 aaccagccaa accaaagcag tcctgtaccg atagcagctg ccataagact gctatgctga
     2941 cttctttctc ctaaaggaat ctttctttga ccaagtgtga ggatacatgc taaagagccg
     3001 aatccagctg tcgtgtggat gactgctcca ccagcgaaat ctctaaatcc catctgatcc
     3061 aaaaatccgc caccccatat ccagtggcaa acagggaaat aaatcagcag attccaaaaa
     3121 ataacaagta taatatagcc ttttaaattc aatctctctg caaaagctcc tgtcatcaaa
     3181 ggaacggtaa tcacgataaa catcagttgg taaagaaaga acattaaaaa tggaatcgtt
     3241 gcaccatgaa acatgttggg gaagcttgta acatgtctca tcaaaaagaa atctgccggg
     3301 tttccaataa caccgccgat atcccgtcca aaggccaatc cgaatccacc aaatacccac
     3361 ataatcgttg ttatcccaac ggagagaaaa gattgagaca tgatcgttag tatattattc
     3421 ttccgtgcta atccgccgta aaaaaaagct agaccaggag tcatcaggca aaccattgct
     3481 gtacataaaa tcatgaatgc tgtatctcct gtattgatag gtaatgtatt catttttttc
     3541 gcctcctttc tacgattacg agtataaaag aaggtgtagt tgtttttttc ttctgttttt
     3601 ttgaattaat acttgcattt ataaaagaca gacaattaat aaaagcttga tgtgttgtgc
     3661 tctttttcgt ttttcacaca aataaagaca aaaaataaac ggtcttcttt ttatagaaaa
     3721 ccgtttattt tcgtcttttt ttatttttaa agatattttt tcataaggaa taaagaatac
     3781 ttcatataaa atatataaaa aagaaccgca gcgagtgtcg ctacggttct caaatttgtt
     3841 cttcatttca acataagttt ttcttcaatt tgcttataat ttacgtgcaa agaatgaaac
     3901 gactaatact aaaatgattg caccgatcaa tgatggaatg atagccattc ctgcaattgt
     3961 tggtccccaa gttcctagta aagcttgtcc aattgctgaa cctactaaac cagcaatgat
     4021 gtttgcgatg atccccattg atgatccttt attcgtgatt gctcctgcaa ttgctccaat
     4081 gataccgccg acgattaatg cccaaataaa tcccatttta aattcctcct aactgacttc
     4141 ttacttctta ttgtacacgt ggttcgttat tttctttgac tttttgtgca cctttgccta
     4201 cagcagattt tgctttatct gtttggcgtg aagcaaattg tcctgtggat tttgctgctt
     4261 ctgttacttt gtcttgtact gtttcttgat ctttttggta ttcttcttgt gttttgatgt
     4321 ctgcaacatt cacattcact tcgataacat ctaaatgtgt catattgttt acttcagtac
     4381 tgatcaattc tttgatttgg ttgtaaatat cttcgatgtc tttaccgtat tctacgatca
     4441 catctagatc cacagctact tgtttcttac ctacttctgt attgatacct gctgttacgt
     4501 tatctgtgtt gactaatttt tcagccatat ttgagaaaaa tccgccatct aatgtcaata
     4561 gaccatctac atctttcaat gcataaccga tgattttttg gatgactttg tcttcataag
     4621 tcaattcacc attcatcatg cggttttctc ctttttcatt tgccaaaact gatccatttg
     4681 ttgtattcat gtttttgttt tccatctttg tttcctccta tgatttaaaa aattgatcaa
     4741 ctattcctga ctgtttcaaa taaaaaccga ttcctgcgcc aattgctgtc atgattatga
     4801 tcaacaatgt cttgaaaaat ccaatcgtta accataaaac agctagcagt agtccaagac
     4861 ccccaaagat aatagggagc ttgtatgctg aaaatagttc tttcattatt tggccctcct
     4921 attccactcg agagtgcttg tagtctcttg tatcttcttg ttcttcataa ccgctgtatg
     4981 tgactgccac tttgaccgtt tcttttgttc cgagtacttt agttacttct tcttcgatat
     5041 cttgcatcaa tacacctgtt ttccctacta atgaagaagt tcgtttcaag tctccgttga
     5101 catggatatg gattcgattt ttcgttgaac gtatccgtac tttaggtgaa tgaatgaact
     5161 cttcttcgtg taaatgggaa cgtaccaacc cttcgatcgc ttttttgtcg attgttagtt
     5221 tgccatcttc ccgtttcata acgaaagttc ctcttgactt tggatagaaa agaacgacta
     5281 gcaagatgat aatagcaaga acggcaaata ctacagctgc ccagaaaata tattgttgca
     5341 tgtagtaatt cgttaaagta aagaagcgaa atcgttccaa ttgttcaggc ataatatacg
     5401 gcgcattgat caacgcaatc gctagaaata ctgaaagtag cagtaaactc atgataacac
     5461 cgattatttt taacccctta ttcattttcc acctcctata aacttatttt taccctgtaa
     5521 ataactctac cgacaatcac agtatagata gcgaacagtt agcatagcat cagtaaataa
     5581 agcgctacca aacattgctg gatcataacg gcgttttatt gccagtctga tactgaaata
     5641 acaattgctt acttattctc tatttacttt tgattgctgg ctatacttaa aggatattat
     5701 tcaaga
//
LOCUS       DAFFSV010000143         5670 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFFSV010000143 DAFFSV010000000
VERSION     DAFFSV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.144"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(58..351)
                     /locus_tag="LR110_002573"
     CDS             complement(58..351)
                     /locus_tag="LR110_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBL4823502.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(379..1044)
                     /locus_tag="LR110_002574"
     CDS             complement(379..1044)
                     /locus_tag="LR110_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBL4823503.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1184..1816)
                     /locus_tag="LR110_002575"
     CDS             complement(1184..1816)
                     /locus_tag="LR110_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4823504.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1823..2890)
                     /locus_tag="LR110_002576"
     CDS             complement(1823..2890)
                     /locus_tag="LR110_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL4823505.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2887..3618)
                     /locus_tag="LR110_002577"
     CDS             complement(2887..3618)
                     /locus_tag="LR110_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBL4823506.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3787..4266)
                     /gene="greA"
                     /locus_tag="LR110_002578"
     CDS             complement(3787..4266)
                     /gene="greA"
                     /locus_tag="LR110_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBL4823507.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4402..5577)
                     /gene="mltG"
                     /locus_tag="LR110_002579"
     CDS             complement(4402..5577)
                     /gene="mltG"
                     /locus_tag="LR110_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBL4823508.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         1615 a         1200 c          862 g         1993 t
ORIGIN      
        1 caatattttc aaaagaaaaa agagaaggtg acaaaaattg tgtcaccctc tcttttttta
       61 ttgtttttta aagtgatagg ttggctcctc caggttggga tcataatcaa caagtaaatc
      121 atatccttta aagaaccatt catctgcttc ctcgataaaa aaaagtgtcc catcaatttc
      181 cactttcttt acaggatgtt ctggctgttc aactgacatt ccaatcgaaa aaccatcatg
      241 aacctctgtt ttgccgtaga cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc
      301 aatttctgag tcaaaccact tttgtgcttt tggcgttacc gttaatttca tcccaacaac
      361 ctactttctc ttttctttct attgattcat cttatcgtct agaatatcca catcgcctgt
      421 ctctccaata accagtagat tatcattttc acgaacatat tcatctgctg caggagaagc
      481 gataaccgtt tgatctactc ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa
      541 atttaattca gctaatgttt tgttgaaaaa tttaggattg gagactcgga tttctgctaa
      601 agaatattta tctgacaact ctaggtaatc taaaatattc tttgacacta aattatgcgc
      661 aatccttatt cccatgtctc gctcgggatg gacgacgcga tcagctccga tcttttcaag
      721 tactctggca tgatactcat tttgggcttt tgctaaaata tttggtacgc ccatttcctt
      781 taccatcaga gtaactaaga tactggcttg aatatcttct ccaatagcga caattacatg
      841 atcgaaattt cttatgccta atgatcgaag tgtcatttca tcctgtgcat ttgccacaac
      901 agcatgagtg gctatattca tatattcatt gacccgatct tcattactat caatagccag
      961 tacttcttga ccagcttcga tcagtgttcg gcagatactt ccgccaaatc gtccaagacc
     1021 tacaattgca aaattttgtt tcaccgtttt cctcctttag tatcttcttt tctcagtata
     1081 acaaagcctt tacaaaaaca aaagcaagca tcattattgt aaaaaagcca agaaaaataa
     1141 tagttatttt ctcagctttt gattatcctc tttatcatcg tttttatttc accaaaccat
     1201 gtttgaatgc atagatagcg gcttgtgtac ggtcttccac ttctagctta gccaatatat
     1261 tggaaacatg tgtttttact gttttcagtg taataaacaa ttcgtccgct atttcctgat
     1321 tactttttcc ttcagaaatc agcattaaaa tttcattttc ccgattcgtt agttcttcat
     1381 gtaatacagg ctcatttcgc cgactcattt tttccatcat ttttgttgtc acttctggtt
     1441 ctaatactcg ctcgccacgt tgcgttgccc ttatagcatc agcaatttca tgtgcggtag
     1501 aggtcttcaa caaataccct gctgcacctg cttcaatggc aggataaact ttttcgtcat
     1561 caataaaact ggttacgata atgattttag cttctggcca atcttttaag attgcttttg
     1621 ttgattcgat accatccatt tcttccatga ctaagtccat taaaatgacg tccggtcgaa
     1681 gtgtcatcgc cttctcatag ccttgtcgtc cattttcagc ttctccaatt acttcaatgt
     1741 cttcttgaat agataaataa gaagagacgc ctaagcgcac catttcatgg tcatctacta
     1801 ataaaacttt tatcatcctt atctatgcct ccttcatcaa agggaccttg atttctacac
     1861 ttgtaccttg acctttaaaa ctaatgattt ttactgttcc accaatacca gctactcgtt
     1921 ctttgatatt gttcaatcca taacttcccg ttttcgtttc attcatatta aaccctgttc
     1981 cgtcgtcaat aatacgtaac aaaagattat tgtctatttt gtgcaaatat acctctaatt
     2041 cattggcttt agcatgtctt aatgtatttg ataacaattc ttgaacgatt cggaacaagt
     2101 gatcctcaat ggaactagat agttttacat cttctacatc ccatttcagc gaaatctgga
     2161 ttttattttg caattccttc aataactgct cgataccttg ctttaaactt ttctcttcta
     2221 aattgactgg acgtagatga agaagcagcg cacgcatctc agactgggaa gcgttgatga
     2281 tctctgctac cattttcaac tgcttgcgaa acatttctgg tgtctcgctt ttttctgctt
     2341 gctccgtcaa tgccgacatc atcatcattg ctgcaaacaa ttgttgcgaa actgaatcat
     2401 gcagctcacg ggctaatcga tgtctttcta attctaaaat ttcttctttg gtttgtccat
     2461 ctacatattg gggacggctc gttacaattt gtaattcact agatacttca atcatttttt
     2521 cttttatctt agtgatttcc ttatcaatat ctttgatgta taaatcgtcc gatgcacttg
     2581 ggatcggaag aagcaacaat ttggattcat aattgccaga agaaagtgca cgcagctttt
     2641 cttcgatttt cccgtatttt gtcttttgaa taagtgataa taatagaaag acaattaatc
     2701 ctacacctaa ggaaatggct aacaaatgga agataagcgg aacatataat aaacgtgcac
     2761 gcagtaattc tccccaccaa tgactttgat tgctggcata aaaataagtg aaaagtgaaa
     2821 atagaataat aaggaaagcc gcaatccccg aataaacagc tagcatcgct ctggatattt
     2881 ttcccatcat attcgtatca cctcgacatc tccaatcaag gtattagtaa taatcttcaa
     2941 tctacgaggg ttttcatcat aatcactgct gtagattttg atttgttcat ttttcaaaga
     3001 aaattgctct tcttcaaaca agacatttcc tactaatgtc gcatgctcta atcggatggc
     3061 tacgcccaaa ggtactaaga tccgtgttct gcctatccct tttcttacaa tcacaatatt
     3121 atcatctttt ggcaaaagcg tattgcccaa atctatgata gtatcaccag aaatgacggc
     3181 gatgttgata tcgtcccatt catagacgtc gtttccaata cgttggttac cgaataattg
     3241 ctgtcttttc cgctcattat tatgtgtttt taattgctct gtttggatca tcatgatctg
     3301 tttctttcgc caaaaagcat ttttggttaa atctatccct gaaatctcta cacctttcag
     3361 accaataaat aaaacggcaa atacagccat catccataaa gcaggactgt tgactaagct
     3421 aaaaaaaatg accaaacttc ctaatatcaa ttgaaaattt tgaaatttag tacgaggatg
     3481 tttgcgcata gctagatata tattgaaaat accgaatatc actaaaagca aaagtccagt
     3541 attgtttacg atttgccaaa cagcaaataa cagcagtagt gcttcaacta ccacaaaaaa
     3601 acgccaagaa ctattcatcg ctctttctcc ctcctgacac attctcattt catcatacct
     3661 atttttttaa ctgttggcta taggacttta gatgtaagat cgtttgttga aatctaaaat
     3721 acacaaagag aaaaagatct agagtgtatc atcagtaaga tgaactctct agatctttca
     3781 gtcagactac gcttgttcta ctttgacgat tttaactaac atatcgccac ctggagtagc
     3841 aatagttact tcatcatttt tacgtttgcc aatcaatgct tgcgcaatag gagaatcatt
     3901 ggaaattttg ccagaaaacg gatctgcttc agcgcttccg acaatcgtgt actcttcttc
     3961 ttctccatct ggtagttcga tgaatgtaac tgttttacca attgatacct catctgcatc
     4021 tacaccgtcg ttatcgatga tttgagcaaa acgaatcata ttttcaattg ttgtgattct
     4081 tccttcaaca aatgcttgtt catctttcgc tgattcatat tcagagtttt cggaaagatc
     4141 tccgaaaccg cgggcaattt tgattcgttc aatgatttct ccgcgtttga ctgtttttag
     4201 ttcttctaat tctttttcta atttttcttt accttctaga gtcattggat aaactttctc
     4261 taccatgtgt aacgtctcct tttttcgata aaataccgca aaaggtaatc ttttgaggaa
     4321 tatagtccaa aaaagattac cacgcttttc ttcaaatgta aatgtttttc taaaaatgat
     4381 caatgtctag agcctgtttt tctaagaatt atttacatat ttttccacta attccatatg
     4441 ttcttcataa gttttagcga aataaacatt tccagtgctc gtatctgcta cgaaataata
     4501 atagtcattt tctgctggtt ctaatactgc cttgattgcc tcttcgcttg gattgtcaaa
     4561 aggtccaggt ccatagccgg tattggtata caaattatat ggtgaatcta cagctgtatc
     4621 ttcatacgtc actaattctt tgtgttcacc taatgcgtat aaaacagaga tatctgactg
     4681 aagtggcata tttgctttga ttcggttaaa gaagacttgt gcaatgtttt tacgatcatt
     4741 ttcttttacg ccttcttttt caactagaga agctagagtt aaaacttctt gtacagtcaa
     4801 atttttctgc ttgatagctg aataatagtt ttgcatgacc gtattcgtct tgttcaccat
     4861 ttcaatcact agatccttaa gcgaagtact cgtgtaataa tcatatgtgg ctgggaataa
     4921 gtaaccttcc aaacggtatt ttgtgttctc agctttagat gcactttcca acagttctgg
     4981 gaatttttgg tgcaactcgt taaagaaagt ctcatcattc actaaatcta aaaattcttt
     5041 cttgtcttta ccagttacct tcgctacccg ttcagcaatc tgatcgatat cgtacccttc
     5101 tggaatggca attttcccat ctgcaacttt tgttggctca gaagtaccgc cttcttgcaa
     5161 ctgtttgcca atctcgtcaa gagtcatatt aggcgctaac tgataatagc cagcttgaaa
     5221 gccagttaaa tttttaaatt ttgtataata attgaaaaca atcccgcttt taataatatt
     5281 gtccttctcc aaaatctcac caatctgctt gttagaagag ccactcggga tctcgacttg
     5341 gactaattgg tcatctgatt tatctaaagg cttcaacccg ctatctacat aacgatacac
     5401 ggtaaatcca agaaatccgc caataatcaa taatgtaaga gcaatcacta aaatgatctt
     5461 cctcacaatc ttatcttctt ttttacgcag atttctccgc ttgtcgatta tatctgctgt
     5521 agctgcctta ctggcttctt ttttatcttt acttgggtgt tgtttgtttc ctgacaaaac
     5581 aactcctcct tactatcaag ttcgtaactt tattcattat acatgatatt tgaattggcg
     5641 tgaatgattt tatttaaata ttctatcaaa
//
LOCUS       DAFFSV010000144         5636 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFFSV010000144 DAFFSV010000000
VERSION     DAFFSV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.145"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(26..189)
                     /locus_tag="LR110_002580"
                     /pseudo
     CDS             complement(26..189)
                     /locus_tag="LR110_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
     gene            complement(542..1231)
                     /locus_tag="LR110_002581"
     CDS             complement(542..1231)
                     /locus_tag="LR110_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL4823509.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYIGHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1294..2058)
                     /locus_tag="LR110_002582"
     CDS             complement(1294..2058)
                     /locus_tag="LR110_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBL4823510.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIVKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(2086..2784)
                     /locus_tag="LR110_002583"
     CDS             complement(2086..2784)
                     /locus_tag="LR110_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBL4823511.1"
                     /translation="MARTQKQKAAQTHVVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTIAANIAVVFAKSGQRVLLVDADLRKPVVYETFQLNNAS
                     GLSTALSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYRN"
     gene            complement(2796..3566)
                     /locus_tag="LR110_002584"
     CDS             complement(2796..3566)
                     /locus_tag="LR110_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBL4823512.1"
                     /translation="MEETISLKEIFGTLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTAGQI
                     KDAISVNQSENSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIISGAV
                     ANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILGTVP
                     EMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(3582..4526)
                     /locus_tag="LR110_002585"
     CDS             complement(3582..4526)
                     /locus_tag="LR110_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL4823513.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4572..5351)
                     /locus_tag="LR110_002586"
     CDS             complement(4572..5351)
                     /locus_tag="LR110_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823514.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKERVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNINY"
BASE COUNT         1509 a         1161 c          946 g         2020 t
ORIGIN      
        1 agatattcct caattttatc tcttattatg ttattatcag ttgataccat tccattaatg
       61 tttcagaata attacaatat tttgggaccg gataactccc ttttgctcgt aaccactcat
      121 atccgttagg attcactaaa agtttaccgt ttatttttcg attctttttt tgaaccgtcc
      181 catgaacata ccgattcaac aagctaaatt ataaaaattg gtgtgctgtc attatttttt
      241 ggaaaattta ttaatggcta tcaatatact tttgtatcat actaaatagc tcgtcagttc
      301 ctatatcgga tataccaata ttaaagcatt tagatcatta tatttaagag tggtatcatc
      361 ccaaaatttc gaatattttc ttttatataa gaataatgat actgaattgc cccattcacc
      421 tttctttttt tagattttct acaagtgatt caaaccctcc ataattacag ttattacttt
      481 tgaaacgata atatgaacat gtgtaatctt ttttattaac aatccatcat tctatcccta
      541 actaatatgc tccattgggc ttgacaataa cataaaacgt ttttaaaatc aacctcaagt
      601 cgtacattaa acttcgatga ccgatatact ctaaatccaa ctcaaccatt ttatggaaac
      661 taacattatt tctatcacta acttgccaca aacctgaaca accaggtttt acatacaagc
      721 gttgaagatc atatctcgtg tactcagcta cttccctctc caaaggtggc cgtggaccaa
      781 ttagactcat atcgcctttt aaaacattcc atagctgtgg caattcatca atgcttttgg
      841 cacggataat tttacctatt ttagtgacac gcggatcttc tttcatcttg aacattgcac
      901 cttctatttc attttgatca agtaattctt ccaatttttc ttcagcatct aaacacatcg
      961 aacggaattt atgcattttg aatgtttttc catttttacc aacacgtgta tggctaaaaa
     1021 atattggact tcctggctct tcttttctga ttcgataagc tacatagaac atcaaaggag
     1081 aagctaaaat caaaccaata atgcttccta caatatcaat cattctttta aaaaataaat
     1141 agatatatcg tttttcaact ttttctttat ccaaccaaac ttgattagga tgtacttcac
     1201 taatacgaga taataaatct tcactttcca tactttcagt ccctttcact atcttcactc
     1261 tctcctcatt tgtttttatc tacgaataaa gaactaaaat aaaccaaact tcttcttttt
     1321 tacctcttca tagattggat aactgatctc atccccgttg atcagatcct ttgcgacttg
     1381 tttcatctgt actacttttt ccttaccaaa gtccttgaca atctgctcat agcactcttt
     1441 taagtaaaag gtgcgcttag agaccccgtg tgcatcagaa gcagccatct gcacgagatt
     1501 atgtttgacc attgttttgg ctgttttctg gatgtctttg ccaaatacac caacataact
     1561 tggcgcagta atttgggcca aacatcccat atctaggaac ggaagcaaat gatttgggtc
     1621 ttctcgaaaa tgcgcgttgc gttctggatg gacgatcact ggtgtctttc ctagttctaa
     1681 caactcaaaa aaaagccatt ctgcatataa cggcacttcc agtgtcggaa attcgatcag
     1741 aagatatgta tcatctaaat ctgtaaataa aatttcatct cggtggatgt cttcgatgag
     1801 cgttccggta atacgtactt cttgtccttc caataacgtc aaaggcaact gtcttttctc
     1861 taattcggct tgtaacgaag cgactagaga aatgacttgt gatttttcat tgctgtattt
     1921 tccattattg tggtgtggtg tgcataaaat atgcgtgatg ccttgctgaa tcgctttttc
     1981 agccattgca atacttgctt ctaagttttc tgccccgtca tcgattcctg gtaggatatg
     2041 gcaatgtaga tcgatcattt ctcttgtctc ctctcttctt attccttaat ttctgtagta
     2101 ataaccagca tctttgctat gttctgctcc gttgtataca acacctaaga ctcgtgcttg
     2161 taccatctct agcagttctt ttgctttagt caaggattct ttacgtgatg tattttcacg
     2221 tacgaccaat aaagtgccat ccgtttttga agacatgatc tgtgcatctg ttacggctac
     2281 tactggtggc atgtcgaaga tcactacatc aaatagttgt cttgcttctg ctaaaatctg
     2341 atccattctt ggcgagctta ataactctga tggatttggt ggtttaggtc cacttggcag
     2401 aatcgacaaa ttctcgactg gtgttcgttg gatctcatct gctacacttc ctgatgaact
     2461 aagtgctgta cttaatccac tggcattgtt cagctggaat gtctcataga cgacgggttt
     2521 acgtaaatct gcatcgacta gtagaactcg ttgaccggat ttagcaaaaa cgactgcaat
     2581 gtttgctgca atggtggatt ttccttctcc aggacctgat gaagtcacga caatcgtttt
     2641 gatttgctga ccagctgcgg aggcaaactg gatattcgtc cggatcgtac gatattgttc
     2701 ggcgattggt gaagatgggt ttgttaatgt gatcaaactg actacatgag tttgtgctgc
     2761 tttttgtttc tgtgttcgtg ccatttttct tcctcctaga ctcttgagcg gcttctgcgt
     2821 gatggagctg ttttatcgtt tgtagctttt ggtcgtgcag gagctgatcc ttttttacgg
     2881 ataccagcgt tcaattcttt cgctgacatt tctggaactg ttcctaagat tgggaatcct
     2941 aatgtatccg tcacatattt ttcatctttc actgtccgat caagtaattc gaataagaat
     3001 gccaaaccta cgcctaccat cattcctaat actaagccaa tcactagatt caatttatta
     3061 tttggcgaaa caggtgtcgt attggcaact gcacctgaga tgatcgagat tttatcgaca
     3121 ttcatcacgt cttttgcgtt ctcttggaaa acttgtgaag ttgtattagc gatctgttga
     3181 gcagtcactg cattcgtgct tattgcctgg atcgagaaca tttgtgagtt ttctgactga
     3241 ttgacggaga tcgcatcttt gatttgtcca gcggtcattt tcacattgtc ttctgtttct
     3301 agacgatctt tgacttcatt taatactaga tcactaacga tcatatcttt atacgtattg
     3361 atcatttgca ggttcgtatt gacattcgct gtgtctgatt gcggcagtgt cacgatcaat
     3421 tgtgcttgtg aactgtattt tggtgtcaag atgaaaaatg ttacgattcc tgagatggcc
     3481 aagccagcaa acattgagat gatgattgtt gccaagtgtt tgcgtaatgt tccaaaaatt
     3541 tctttcaagc tgattgtctc ttccattttt tctccacttt cctatttatt ctctagattc
     3601 atttgatctt ttaattcttt tttcacacga tcaagttctt gatcgctgac tttttgatag
     3661 gagatattat cttgcatgaa gccttctcct tgcagctgat cttgcttgat cgtaccaaat
     3721 gcctcccgat aatctaaagc gatttttttc atttcatcaa agctcatgtc tgttttcata
     3781 ttcgattcta gagcattcaa aacggattga tagttgctga caccgtttag actcaatact
     3841 tttttcgcta caccttctac gatctttctt tgacgctctt gtctaccata gtcaccattt
     3901 ggatcttcat aacgcatacg agaataagat aatgcctgat ccccgttcaa tgtgatttta
     3961 ccgattgtaa agtcatagcc atcttgtgag aaagtgaggt cattattgac ttcgatacca
     4021 ccaactgcat caacaagttc ttttagccct ttcatattga tagagacata gtgatcgatt
     4081 gggatgtcta catatttttg tacggtatcc atcgccatgg cagctccgcc gaatgcgtaa
     4141 gcgtggttga tcttgtcttt tgtgtcacga cctacgatgt cgacatacgt atctcgagga
     4201 atgctgacga tcgttgtctg tttgtcttca ggactgactg tggcaaccat catcgtatct
     4261 gatcttcctt gatcgacccg acctaaatct ccagtatcaa tccccatcaa tagaacagag
     4321 aaagattctt gtttgtccag atcaactggt ttcgttcgtt ctccttgggt tcgttccact
     4381 gattcgtaag ttttctcaat agaatgtgag agatccagat ataattttgc tcctactcct
     4441 acaattgcta atactaatac gataagaact gctaaaataa tcagaaatat ttttttccct
     4501 cttgattttc ttcttcgtct tgccatagtt tcctccgttt gctaaccttg cttgaatcat
     4561 cgctttaact gtcaatagtt tatattgcta tagcccattt gctgagcaac agtttgtgct
     4621 tttgttgcgt atgctacgct tattttttca ttccggatct gctctatttg actgagcgcc
     4681 gtcaaatatt gatcgtaagt agcggtggca gtcacttgac catcctttaa catcgtatcg
     4741 aataactgat ctagttgcgt gacttgcgcg atctgggtat cagcgaatcc tagatattgc
     4801 ataacaaccg ttttccaagg actatcttta aaaaagctca tattttcacg tacacgagcg
     4861 acatctgcac ttgtcaccct ttctttgatg atgacatcgt ctacagcttg ctgccagttt
     4921 gatacatttt tagtaaataa atcgttgaca gctgtctgga tcttcagttt tgattctgca
     4981 ctatctaact gtgtcacgat tgctttcttc tcatcttgga ttgcaacagc ttctttaggc
     5041 aattctgatt ctttgatgtc gtaatcatct gctgaaagtt tgacagagga tagcttattt
     5101 gacaaacttg tgattgtttc ttctgttatt tcagggttta gaaaaatttt ttcttttgta
     5161 ctatacagct tgtctacttc ttttgataaa gaagtcaagg tgtctttttg attggtgatg
     5221 gcagattctg cataattgat ccgttgctga tgttgccgct gattattgat atggtatcca
     5281 aacaaacaga tcgcaatagc acctgcacct accgccacag cagtcaatga cttgacgaat
     5341 ggcttttcca tagattcgct cctaagtatt gatgatagat aaattaagat ataattcact
     5401 tacataaaaa tgacagtttt ttatttaaaa aaacataatt aaactaatat attcacagaa
     5461 atatttgtaa acaagttttc cctatataat cataccacaa actgctttct tgactaggga
     5521 aatttacgat ttaaaagtaa ataaaacatt aataagataa caattaatcg attttacttt
     5581 tatttcctta attttcatag gtgtttttaa aagttacaca aaaaataaaa taacaa
//
LOCUS       DAFFSV010000145         5577 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFFSV010000145 DAFFSV010000000
VERSION     DAFFSV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.146"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            4..2136
                     /locus_tag="LR110_002587"
     CDS             4..2136
                     /locus_tag="LR110_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HBL4823515.1"
                     /translation="MQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTDLEGTSAF
                     GLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKTKGNVLWI
                     ADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWKQKGLVFH
                     IVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVLFEYSRSY
                     LLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDALASATLSI
                     FLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEMAAGAAVN
                     SSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIKHLIQLIS
                     SALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQVANNRLNE
                     IFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPSHSKTAIV
                     GVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQESFFFSGTI
                     IENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGGQRQRLAL
                     ARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAKACDQILV
                     LHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2105..3520
                     /locus_tag="LR110_002588"
     CDS             2105..3520
                     /locus_tag="LR110_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBL4823516.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3553..4587
                     /gene="sppA"
                     /locus_tag="LR110_002589"
     CDS             3553..4587
                     /gene="sppA"
                     /locus_tag="LR110_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL4823517.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4589..5110
                     /locus_tag="LR110_002590"
     CDS             4589..5110
                     /locus_tag="LR110_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL4823518.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5107..5493
                     /locus_tag="LR110_002591"
     CDS             5107..5493
                     /locus_tag="LR110_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL4823519.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         1999 a          761 c         1060 g         1757 t
ORIGIN      
        1 tttgtgcagc aacaagatga aaaagattgt ggtgttgctt gcctttcgat gattttaaaa
       61 tattttaaaa ctgatatccc gattcataaa ttacgggacc tatctgggac tgatttagag
      121 ggtacttcag catttggatt gaaaaagaca tttgaaaaat tgtcttttga ttgtatggct
      181 attcaagcag atagtgagat ctggaaagaa agagatctta tcttaccatt aattgcacat
      241 atattggttg ataacagcta tatgcactac gttgtaattt ataaaacgaa gggaaacgta
      301 ctatggattg cagatcccgc taatggaaaa aatagaaaaa cgattgaagc gtttcaaaaa
      361 gaatggacag gtattctttt attacctatg cctagagaaa cttatattcc tattaaagaa
      421 aaagtagctg gattaagttc ttttttgcct ataatttgga aacaaaaagg attagtattc
      481 cacatcgttt tagcatcgat tttcattacg ttttttggca taggaagctc atattatttt
      541 caaggtattc ttgatttttt catccctaat caagcacgtt caactttaaa tataatttcc
      601 attggcttga tcgctgtcta tttatttcgg gtattgtttg aatatagtcg tagttatctt
      661 cttattattt taggacagcg aatgagtatg gcaattatgc ttcagtattt caaacatgtg
      721 ttaactttac caatgaattt ttttgctacg agaaaatcag gggaaattat ttctcgtttt
      781 cttgatgcca gtaagattat tgatgcttta gcaagtgcta cgttatcaat atttttagat
      841 attggtatgg tgttgcttgt tggggttacg ttagctattc agaatagtac gttgttttta
      901 atcactctat gctctttgcc tttttatgta gttgcaatat tcgctttcgt gaaaagctat
      961 gaaaaatcga acagggaaga aatggcagcc ggtgcagctg taaattctag tatcatagaa
     1021 agtcttaaag gaatagaaac aattaaagca tatagcggtg aagagaaagt atataaccgt
     1081 atagaccatg aatttgttaa attaatgaaa aaatcatttc gtacggttac gcttgataat
     1141 attcaacaag gaattaaaca tttgattcaa ttaattagca gtgcattaat tttatggcta
     1201 ggttcttatt ttgttatgga taatagaatt agtctaggtc aacttatcac atacaatgct
     1261 ttactagtat ttttgacaga tcctttacaa aacattatta atttacaagt gaaaatgcaa
     1321 acggctcaag ttgcaaataa tcgtttaaat gaaattttcg cgattgattc agaaaaatcg
     1381 aaccagtctt ttgaatatat gctttctgaa caattatttc aacaagatat cactgtaaaa
     1441 aacgtttcat tctcgtatag catgaaggcc ccaacgttga acgaaatttc atgtacaatt
     1501 ccttcgcaca gtaaaacggc tatagttgga gttagtggtt ctggaaaatc aactcttgca
     1561 aagttatttg ttcgttttta tgaagcatca gaaggatcga tccattatgg aaacattaat
     1621 cacttagaca ttccttttca gatattacgc gatcgagtga catatttgcc tcaagagtca
     1681 tttttcttta gtggaacaat cattgagaat cttatattcg gtttagatag cccaccaagt
     1741 ttcgaacaaa taatagaagt ttgtgagata gtccaactaa atgaatttat taatcaacag
     1801 ccattacgtt acgagacatt gttggaagaa ggtggaaata atctttcagg agggcaacga
     1861 caacgattag ccttagcacg ggctttatta aaaaatccgg acatattaat tttagatgaa
     1921 gcaactagtg gattagatac cttgttagaa catgcaatta tgaattatct cctcaattta
     1981 aaagaaaaaa cgattatttt tattgcgcat catttaccaa tagcaaaagc atgtgatcaa
     2041 attttagttc tccatgaagg gcatttggta gaaaaaggaa cgcacgatga acttcgtttt
     2101 gccgatggta tctatcaaaa attatgggaa atctaggaga gggaaacaat ttatgcagaa
     2161 taataattgg ttcgaacatt ctagtatcta tactaaacaa catcatagct tctaccgttg
     2221 ggtgttatac cctgtaattc tttttttatg tattattgga ttgtttttaa cctttgctaa
     2281 aaaagaggtt gttattcgga caacggctca attgaacccc gaaaaaatag agaagttaca
     2341 agtgccatta gaagctaaaa taatggaaaa cttcctaaaa gaaaatcaat ttgttcataa
     2401 ggacgatgtg ttggttagat tggattgttc tttaatagag aatgaaaagg ctcagataga
     2461 acaagaaaac caacgtatta ctcaacaaat aaagatggct cagctattta ttgaaagtat
     2521 aagtaaagga aaaaatttgt tttcaacgga tgacagtttt ggctacagta atcaattaaa
     2581 gagcatgttg tcagaaaaag aatcactccg ctacgctttg aagcaaagtg aattaaatga
     2641 tcaaaagcaa ttagaagtat acgaaaagac aaaaagacaa ctagaaaaac aaattgagag
     2701 ttcagatagt aaattacaag aatggcaaca agtacaggta gcttggagta ataatcaatc
     2761 attaaaagat ttttcaaaag aaatgatggc aaactatgag aattggcaag aacaactaaa
     2821 taatgtttct gatgatcaaa aaaatcaagt gaaactgaca atttcagcaa gcataaatga
     2881 acaaattgag caactaaaaa aagaagtaga acagtatcag tcagaaaaag ctaaattagt
     2941 taaaccaact acttctgaga atgacagaat tagtcaaacg gaaaaaggaa agcaagagct
     3001 agaacaagca ataacaactg taaaacaaac agttacagag ctaaatgaaa aacaagagaa
     3061 gaaccagtca gttatcaaaa gtttggacga tcaactttca aaaggaatac taaaagctcc
     3121 tgtatctgga acggttcatt taaatgaaga aacaaaggga caagcagaac tagccaaagg
     3181 aacagtgctt gcagaaattt atccgatcca cgaaaaatca aaaatgaaat ttacagcact
     3241 tttacctgcg aatgaaagta cctatattaa agaagggatg aaggttcatt ttaaattgga
     3301 tcaaaaaggt ggggctccga ttacgatcga cggaacttta gatgaaattt cagaaaattc
     3361 tactgccact gagaaaggtg ttttttatgc agtgaaagga acgttaaaaa caactcaatc
     3421 gtttccgttt cgttatggat taactggaga gttgtcttta attgtaggag aaaaaacata
     3481 ttggcaacaa attgtcgatg tgtttctaaa taaaaaataa ttatttttac taagaatata
     3541 ggaggagttt atatgaataa aagaagatgg attgctgtgg gagtagcgtg cggattattg
     3601 ttactatcca ttatcgtcgc gctaattcca ggaaaagata aagaggaagc atcaaataca
     3661 accctaacag gaataaataa gattttttac ggatctaacg aaatcacaga agaaacgtta
     3721 gaagaaggcg catcaaataa aaagattgtc aaactatccg tcaatggagt tattgcagat
     3781 acaggtgaaa gcaatctttt tagtagggaa caatataacc atcagaattt tttaacacag
     3841 ctaaagaaaa ttcaagaaga caaagcggtt aagggagtgt tattagaagt aaattcacct
     3901 gggggaggaa tatatgaaag tgctgagatt gctaaagaaa tggcaaaaat taaaaagcta
     3961 gatattccta tctatactgc atttaaaaat acagcggcaa gtggagggta ctacatttct
     4021 gctggttcag ataaaatatt tgctacggaa gaaacaacaa ctggatcaat tggtgtcatt
     4081 atttctggtt taaattattc tggcttatta gaaaaacttg gcgtaactga tgcgacgtat
     4141 aaaagtggtg cattaaaaga tatgatgtca cctcaacaca aaccttcaga ggaagagaat
     4201 aaagttatac aggaatttgt gatgagtgct tatgatcgtt ttgtcaatgt tgtagcaaaa
     4261 ggaagaaata tggatactaa tgcagtgaaa gaacttgcgg atggaagaat ttatgatggt
     4321 aaccaagcag tagaaaatgg gttggtggat caaattggtt actctgaaga tgcattagat
     4381 tccctaaaaa aagagaagaa attaactgat gcgacggtta tagaatataa aaatgacaca
     4441 actggctttg cttcttcttg gttaggtaat aagattgctg aatggcaagg gctaaaggca
     4501 actacatcag atcgtgtaat ttcagttttt gaaaaacttg gattttctga tactccaaaa
     4561 ccaatgtatt actatggagg gctataaaat gaataagaag agtggcattg aaactttgcc
     4621 taattatgta tttgcgggat tttggttacg attttttgct tttcttattg atttaatttg
     4681 tattagcatc atcacccatg ctactatagg tctcttttac aaaatcatgg gactggaatc
     4741 tgacaaatcc attattactt tttatggtat agcgtcatta gctatttatt taacctattt
     4801 tgtacttttg actaagctga attcaggaca aacaattggg aaaatgattt ttgggataag
     4861 agtagtgagc tttgaggaaa aagagctaag ttggcaaact atctttattc gtgagtgtat
     4921 atgtaggttt atattaaaaa attttgtatt tcaaattggg tatttggtgg ctgctttcac
     4981 gcccaagaaa caacatattg gggattattt ttctaacacg tcagtggttg ttattcgttc
     5041 attagaagct attaactatg gtcaaaaaat ttctaattct aaaacactta atctggagag
     5101 aattaaatga ataagagaaa aacagagttt attttgattt taattagtgg aataacaggg
     5161 attttatgta gtcttttagc attgatgggt ggtatgtttc tatcttctat tgattcaact
     5221 actttaaata ctacagaact aaccagtaag gatatagata tgttagtcaa tctaagcggg
     5281 aatgttgttt tatttggagg aattacccta gttttttcga taatgatttt cataataggt
     5341 tttttagtaa gaaaaaatca aaaaacagtg gtctatggta ttagtttact cattttatct
     5401 attattccat tttttctttt aacattattg tgggtaattc ctggagtttt aggtattatt
     5461 gcgggattga tgcttctaat aagaaaatct taattaggca atgtgtagac aacgtgaaac
     5521 gattaatcaa ttaaaagcta aatacgtttt gagaaatgat aacttgtagt cgatgga
//
LOCUS       DAFFSV010000146         5481 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFFSV010000146 DAFFSV010000000
VERSION     DAFFSV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.147"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="LR110_002592"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="LR110_002592"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="LR110_002593"
     CDS             complement(580..1353)
                     /locus_tag="LR110_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4823520.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="LR110_002594"
     CDS             1497..1847
                     /locus_tag="LR110_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL4823521.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="LR110_002595"
     CDS             1828..2544
                     /locus_tag="LR110_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4823522.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="LR110_002596"
     CDS             2544..3992
                     /locus_tag="LR110_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL4823523.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="LR110_002597"
     CDS             complement(4062..4571)
                     /locus_tag="LR110_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823524.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="LR110_002598"
     CDS             complement(4561..5286)
                     /locus_tag="LR110_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823525.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DAFFSV010000147         5425 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFFSV010000147 DAFFSV010000000
VERSION     DAFFSV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.148"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..928
                     /locus_tag="LR110_002599"
     CDS             <1..928
                     /locus_tag="LR110_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4823526.1"
                     /translation="NNSSLIKNETKQKIRQLAKEMNYSPNYSAKGLVNQKKYLIGLFF
                     SSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDVVDLTEINLQRYDGLIVMSQSDD
                     DQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAAGVMQAVDYGIGCGHTKIAYIGG
                     KKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYSLESGLQNMEYLLSLSDLPTLVF
                     CGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVVSYLNPPLTTVRKPLKEMCQEGT
                     KLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            988..2409
                     /locus_tag="LR110_002600"
     CDS             988..2409
                     /locus_tag="LR110_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBL4823527.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2402..3901
                     /locus_tag="LR110_002601"
     CDS             2402..3901
                     /locus_tag="LR110_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBL4823528.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            3940..5334
                     /gene="uxaC"
                     /locus_tag="LR110_002602"
     CDS             3940..5334
                     /gene="uxaC"
                     /locus_tag="LR110_002602"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL4823529.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1771 a         1013 c         1123 g         1518 t
ORIGIN      
        1 gaataacagc agtttaataa aaaatgaaac aaaacaaaaa atacgacaac tagctaagga
       61 aatgaattat tcgccaaact atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat
      121 cggactattt ttctcaagtt tgcataaagg aacttcatca aatttcctag cagattccat
      181 tgcaggcatc cattcagtat tagacacaaa ttacagttta tctgttgaaa gtatcgatgt
      241 tgttgattta acagaaatca acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag
      301 tgatgatgat cagcctttca tcgactatct caaagaaaaa gaatttcctt tcgttgttgt
      361 caatcgtcac atcaatgatc cagcggttat caacgtcact gccgatgatg cggccggtgt
      421 tatgcaggca gtagattacg gtattggatg cggacatacg aagatcgctt atattggcgg
      481 aaagaaaaat tttcgttcga caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa
      541 agcgagcatc cctgtcaatc tagcctattt tttaacagga gattacagct tagaaagcgg
      601 cctccaaaac atggaatacc tattatcact ttctgacctg cctactctgg ttttttgcgg
      661 caacgacgac atagcgattg gtgcattgag agctgtacat ttagctggcc tccgtgttcc
      721 ccagaacatt tctctgatcg ggttcgatga ttctccagtc gtctcctatc tcaatccgcc
      781 gcttaccacc gtacgtaagc ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact
      841 agatttgatc gaaggcagga cgatcgaaca atctaaaatt gagctctcca ccttgttgat
      901 ccttcgtgag tcgatcaatt acttgtaaac tacaagtaat tttatataaa aatgttaacg
      961 tgtgcataac acagaaagga agtttatatg aaaaaactga caaaagaatt agtgaaaaaa
     1021 aacgaagcac ctgtaaaagt tcttcagttt ggtgaaggca attttatgcg tggatttatt
     1081 gattggcaga ttcagcagct gaataaccaa caattgttta aaggtaatgt tgcaatcatt
     1141 caacctttag aacaaggtct tggaaatatg atgaaccagc aagataattt atatacggtc
     1201 attttacaag gactcagcaa tggcgaaatc gttgatactt ccgagattat caccgtagta
     1261 gaacaaatga tcaatcctta tgatgaatgg gatgcttatc tatctctagc agaaaatgac
     1321 gacttggcct atatcgtctc caatacaaca gaggcaggaa tccaattcgt ggagactgat
     1381 aatttaacag atactccccc acagagcttc ccgggcaaac tgacagcctt tttataccga
     1441 cgtttcaaat taaacaaagc tggctttacc atcattcctt gcgaactgat tgacagaaac
     1501 ggcgaacgtc taaaagaaat cattttacgc tacgcagaaa aatgggattt agaacctgct
     1561 tttgttgagt ggttagaaaa agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc
     1621 ccaggttatc ctcgcgaaga tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa
     1681 ttgatggtca aagcagaacc attcatgctt tgggtgatcg aagggcctaa agaattggag
     1741 aatcaactac cattcaaaca ggctggcttg aatgtcatca tcacagatga tatgactcct
     1801 tatcgtgaac gtaaggtgca tttgcttaat ggaccgcata cagcgatggt ccctcttgct
     1861 ttgttggcgg aattggaaac tgtagaagaa gtaatgaaag atcctttatt ctccgcatat
     1921 gttgatcagt tattcaatct agaattgatt cctatgcttt ctttacctaa agatgaatta
     1981 gccatttatg ctgaccaaat caaagaacgt ttcttgaatc cttttgccca tcacaaatta
     2041 gaagcgattt cattaaatag tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag
     2101 aagtatatcg aagaacaaaa tcaggtacca ccgttaatca ctgtttcact agctgcgcta
     2161 cttcttatgt atcgaggcga tcaagtaaag ccacatgatg atgaaaagac gataagcgaa
     2221 tttactgatg cttggtctga taatggaaca gctattcctc gattattgca aaacgctgcg
     2281 ctttggggag aagatttgtc tcaaataccg aatgtgacag atacagttca agaaatagct
     2341 gcaaagatcg atcaaaaagg tgtccggtca gtattacaag aaatgaaagg aacgatggtc
     2401 catgcttgaa accctgcaac caacaatgaa attaaatcga cgagacagtg tggtcgtcgc
     2461 attaacccct attccaaaac aaacacaact gacgattgat cagcaaacga tcaccactct
     2521 tgaagatatc ccgcaaggtc ataagattgc attgggtgat ttgaaagctg gagacaacgt
     2581 gatcaaatat ggctatccaa tcggtcatgt gacgacagac gtaactgccg gtcaatggct
     2641 gcacactcat aatgtaaaaa ccaatctttc tggtgaattg gattatcgat atgaaaaaga
     2701 tgtccatcca agtcattacc tttttgagaa ccgaacattt caaggttact tgcgaaaaaa
     2761 tggaaaagta gggattcgta atgatttgtt tttagtacct accgttgggt gtgtgaatgg
     2821 cattgctgaa ctgatcgtca aacagttcaa ggaaaaacat ccagatctgg gacagtttga
     2881 ccatattacg attttaaagc atccttatgg ctgttcgcag cttggaaaag atcatcaaaa
     2941 tactcgagaa attttagcgg acgcagtaaa tcatcccaat gccggaggag ttttagtatt
     3001 tggattaggt tgtgagaaca atacagttcc tgaattcaaa aaaattttag gtgaatatga
     3061 tgaagaacga gtgaaatttc tagtggctca agatgtttac gatgagatcg aacaaggtgt
     3121 ggctctatta gaagagcttt taacagcggc ggaaaatgac cagcgcatat cagtcccgct
     3181 ctctaagttg aatgtcggtt tgaaatgcgg cggttctgat ggtctatcgg gaataactgc
     3241 taatccttta cttggtgcat tttctgacta cttgattgct caaggaggca gcacgatctt
     3301 aacagaagtg ccagaaatgt ttggcgcaga acaagtattg atggcacgag cggaaaatga
     3361 agaagttttt gactcaatcg ttgacttgat caatgacttc aaacagtatt tcttgtctta
     3421 cggggaacca gtttacgaaa acccatctcc tggtaataaa gcaggtggga tcactacatt
     3481 agaagataag tctttaggct gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct
     3541 acaatatggc gaaaaaatca gaaaaccagg gcttagttta ttgcaagctc ccgggaatga
     3601 tcttgtagct gcctctgctc ttgcttcatc cgattgccag ttggtcttgt ttaccacagg
     3661 aagagggact ccgtttggct catatgtccc aacggtcaag gtatctacga atacaactct
     3721 ttttgaacgc aaaggtcatt ggatggattt taatgcaggc gtcttattag aacaaccaat
     3781 ggaagtaatc ttagaatcgt ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac
     3841 gaaaaatgaa caaaatgacg ttcgagaaat tgccattttt aaaaacggag taacactttg
     3901 attttcataa aacagtataa aaagaaagaa ggaagattta tgtttttatc tgatgatttt
     3961 ttattaaaag acgattgggc gaaaaagctt taccattcct acgcaaaaaa catgcccatt
     4021 attgattatc actgtcactt agatccaaaa gaaatttacg aaaacaacaa tttcagcaat
     4081 ctgacagaag cttggttagg aggcgatcat tacaaatggc gcttgatgcg agcttgcggc
     4141 gtgcctgaag aaaaaatcac aggaaatgct tctgattttg aaaaattttt agcttggtgt
     4201 caaaccgtac caaaaattat cggaaatccg ctttacagtt ggtcacatct tgaattgaag
     4261 cgtttcttcg gtattgactt gttgataaat gaagaaaatg cagagaaaat ttggacacaa
     4321 gcaaatgaaa ttttggcagc tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat
     4381 gtagaagtga tttgtacaac agatgatccc gcagacgact tgcactatca tcagttgctt
     4441 gctcatgaag aaccagattt gaaggttcta ccttcattcc gtccagataa agcactcaat
     4501 attgaaaaag aaggatttaa tgcatgggtc acactactgg aagaagcagc tgaccaaacg
     4561 atcaccacct atcaagaact gatagaagtc ttagggcagc ggatagaata cttccatcaa
     4621 catggatgtc gtgtttccga ccacgcttta gacatcttac gctataaaga agcggatgaa
     4681 acagtattag aagagatttt tcaaaaagca cgagctaaca aactattatc ttcagatgaa
     4741 atcgatgctt atcttacaga aacactgata catttgaccc acttctatca ttctcataat
     4801 tggacgatgc agctgcacat ccatgcttat cgtaactgta atacacaaat gttggaaaaa
     4861 ttagggcccg ataccggcta tgatggaatg aatgatcttt ctttgactat tcctctgcaa
     4921 aaattattga accgtgcaga agaaacggat cagttgccaa agacgatcct ttattcattg
     4981 aatccgaatg actatcctgc gattttagct ttgatgggct gtttccaaaa agaacagaat
     5041 ggaaaactgc agttaggttc tggatggtgg tacaacgata ctcgagctgg tatgcgcgat
     5101 caattgacac aatttgctga tggtagtgct ttggggaatt ttgtcggcat gctgacagat
     5161 tcaagaagtt tcctgtccta cactagacat gaatatttcc gtcgtgttct ttgtgaattt
     5221 ttaggcgaaa tggtcatgcg aggagaagca cctgaagatg aacaactttt aggaagtcta
     5281 gttcaagcga ttagttatac aaatgctaaa aattactttg gcttttttgc ctaattgatt
     5341 aatagtaaca gtaacgaatc aaaaacagga tagtgaaata acggaatccg tttatccacg
     5401 ttttagagaa gaagacggac caaat
//
LOCUS       DAFFSV010000148         5411 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFFSV010000148 DAFFSV010000000
VERSION     DAFFSV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.149"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(489..863)
                     /locus_tag="LR110_002603"
     CDS             complement(489..863)
                     /locus_tag="LR110_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL4823530.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(886..2286)
                     /locus_tag="LR110_002604"
     CDS             complement(886..2286)
                     /locus_tag="LR110_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBL4823531.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2290..2700)
                     /locus_tag="LR110_002605"
     CDS             complement(2290..2700)
                     /locus_tag="LR110_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL4823532.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2718..3209)
                     /locus_tag="LR110_002606"
     CDS             complement(2718..3209)
                     /locus_tag="LR110_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4823533.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3219..4010)
                     /locus_tag="LR110_002607"
     CDS             complement(3219..4010)
                     /locus_tag="LR110_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL4823534.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(4003..4776)
                     /locus_tag="LR110_002608"
     CDS             complement(4003..4776)
                     /locus_tag="LR110_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4823535.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4932..>5411)
                     /locus_tag="LR110_002609"
     CDS             complement(4932..>5411)
                     /locus_tag="LR110_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL4823536.1"
                     /translation="TTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELV
                     KLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERI
                     MIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1723 a         1084 c          720 g         1884 t
ORIGIN      
        1 agaaaacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg
       61 tactgttcta tggggcgctt actctaggag tgagcatttt tcgaaaaata ctaaaaattt
      121 ttcaaaataa aaaaaggcat acagacaata aactgtctat acgcctcaca gactctattc
      181 tatacatcta attttggctg tcctgcaaaa aatatgtgtt agcctccatc gaaagatcaa
      241 actttggaaa acatcattat aactttgctt ggtaagagct ttgctaaaac atgatatgac
      301 ttgtatacaa aatgtggagt gtattccaat aaataggaag gattacaaaa tttgaaacca
      361 aataagtaaa aaatactgtc actttgaatt aatttgaaat agtaaattct cattaaacta
      421 ttgaaatgct gataccaaaa aatgaagtac ttttcgttca taccaactta gagcacttcg
      481 ttatctattt atttcttcat ttggcttatc tctataatct catgatttgg tccgtagaaa
      541 ttaaagtaac ggattccatt ttcccagaac ggtaattcca taatattttt atctaataat
      601 tcatatccta aatcttgaat tgaattgaat acctcatcta tgtcatccgt atctaatgct
      661 agatgatcaa tggctcctct acactcagta gttttatctt cccatatttc tattatcgtt
      721 ccattcaact cgaaaaagcc gactttactt cccacattca tttcttcatt aatcagacta
      781 aaccctaagg attcataaaa gatttttgaa gctccaaaat cttttgttgg aattccaata
      841 tgttgtaaac catttaattt cacttacatc atcccttcaa ttgttttaaa atccacattc
      901 ataatttatt gaatagtcta gtttatctac cttgattgct ccctccgtaa cagtcatact
      961 taatgctgtt ccgactttat catacattct agtacttaaa gcaacattat caatatataa
     1021 aacagctatc gtatcatcaa caattaactt tagttcatat tctttgccta tttccaaaaa
     1081 tagtggtcgt tgcagcccct tatcaaacat ttgaggccaa ggatagttcg gagtttttcc
     1141 gaaagtaagc tgattattaa tcaatgaaat acgaaattcg tatccttctt gagtatcact
     1201 atctacaaat aattttaaag caaactcctt tgttaacgat gtaatagtca aagttgtttc
     1261 aaatagaaaa taatcttcga ttttaggaac aattactctt tctttttttc caaattcttt
     1321 ctctaactta aaacttgaaa atggagtgat ttctttaaaa ttctcaatta ttttttcagg
     1381 cagtttaaca ccaagagttt tgtctgtctt ttgaataatt tgatgtggaa caagtgcacc
     1441 gccccaatcc cagttatttt gatcgttctc cccttctttt gttgccaccc agccagttag
     1501 aaaacggcca gtttcattag ttccgactgt tcttgctgca taataggctt ttccgtcgaa
     1561 agcttcgtct actggtgatt tccaatctga aaataatcct gctccgactc gatatttagt
     1621 ctttttatct tcacaatact ctgaaaaaag taaataccac gttccatcaa tttcaaatat
     1681 atcaggcatt tcaatcatgt taaattcatt attagcccag tagtctcctt tgaaattcca
     1741 atcttttaaa tcttcggatt caaaatagac caatctcccc gtcggctttt tcttacttgc
     1801 tggttttcta gctcctagta tcaaaacgta ttttccatga tcttgatcaa aaattgcaaa
     1861 aggatcgcgc caatcattct tatcatatcc ttcttgagga acaagttttg ctgcttcgcc
     1921 atcctttttc caatgaatta aatctttaga acttgcaatc attaacactt ctgaagtttt
     1981 tccatccaac ttagctttgc gattatgtcc agtatacaaa gctaaatact cattgattcc
     2041 ttggacaata cttccagcgt aaatgtattg atcaatgtca tcgttgccac cacgatgaat
     2101 tacttccccg tattcttcaa atttcacaaa atcagtggtt gtaactagtg accacccaaa
     2161 aggatcagtt aaaggtttag gatttctcgt atctcgttga tgaaatagat aaaaagttcc
     2221 atctttataa aaaggcatgc aatccccaaa ccaaaaatct ttaggcttgt aaaaaatttt
     2281 ttgcatttat cactcatcct cttcgttagt atgtctttgc atatctgcaa gacacgcaat
     2341 tgtattcata tttcttagat tttcgatgat atcatcgctt ttttttcctt gtaatctcaa
     2401 attgacagcc tcaatcaaaa ttcctaaatt catgccacca agaagttgaa acccattttg
     2461 aaatacttca cttcttttag caaattcgtt gaaaggtgtt ccaccaatca agtcagcaaa
     2521 taccataact tcttgatttt ttacttgaga aactattttt tcaatgtttt cacggaattc
     2581 ttcaatgcct gcatctccaa gtgttaccac atgacaattt aaattatcgc caaaaaacat
     2641 ttttaatgta tctacaaatc caatggcaat atttccatga gatgtaatta taaactccat
     2701 ccactcatcc tctgttctta ataaataaaa tcctttaaaa attcttttga atctctagga
     2761 gtttgtatca ctgtcacttc aactccatca ctaattagtt ctttaaaagc ctctctctct
     2821 tcttcagtaa tatttatcgt cggacggatg cgttctgttc cattccttct tgataaattt
     2881 cctacgttta cacttgttat tggtaaaccg ttatcaatca aaaatttaac atcgattgga
     2941 gaaactgcaa caataagaac tttttgtcct tcatatttac cagaaagaat atttttaact
     3001 gctacttccc ttgtaattat tgaagttcta acattaggtg gtgcagcgag gcgaactaca
     3061 gcttttttta cgtcatcatt ggctacttca ttattaataa ccataattct gttagcactc
     3121 aattgatttg tccattgtgt tgctacttgt ccatgaatca ttcgatcatc aatacgaaca
     3181 tgtataatac ctttcatttt ttcatccata ttcaacacct acttcaaaat atttaacgct
     3241 cttaacgcaa tagaaactac taagattaat agaattacat aaactggttt aacctttcgt
     3301 cctaacaaat aatacacaat tgctactaca catgcaggta ataaaccagg cataacttga
     3361 tcaaagattt catttccggt cattgttaag tcgccttgtt taaattcaaa gccaacattc
     3421 acttttacaa ctgtggcaat gagtgcaccg accaccatta accctaaaat tgatactgca
     3481 tcagtaatag actttaattt tgtttcaatt tgagttagta ggctcgcacc cgattctttc
     3541 ccagcaaggt ataaaggata agcagtaatc cttaataaaa ttgatgccag tatccacaaa
     3601 ataattccaa caaaactacc ttccaatgcc atattagctg caattgctcc aaaaatagtc
     3661 catggaataa ctacaaatac actatctcca attcccgcta aaggacccat tagacctgtt
     3721 ttcagagcac ttactgcttc caaagactta tttttttctt ctttttcaat tccaacattc
     3781 ataccaataa tgtaaggcgc tgtatacggc gtagtgttaa aaaattgtag gtgagctaaa
     3841 acggattgtt tcattccttc aggatcatct ttatatgtgt ccttgataaa aggaagcata
     3901 gaatacgcat aacccaaccc ttccattttt tcataattta atgaagttgc accattgaga
     3961 atccaccgga aaattacttg tttgtcgatt tttgtcagtt tcttattcat cttcgtcaaa
     4021 acctcctgtt gcagctggtg ctacttgagt attatttgat ctatttttat atacgattag
     4081 tgcgattgca gcgccaagta acgaaatccc taaaattggt atttggaaat aaactgccaa
     4141 aacgaaacca actaacaaat aggcaaagta atcttttgta ggtaaatatt taagtagcat
     4201 tcctatccca acagcaggta atagtccccc tgctaccttt aaaccaccgg taacccattc
     4261 aggaatataa ttgattgcac ttcccactaa cgttgggcct aatatcacag ttaacaaaac
     4321 aggaattcca gatacaaagc ttgtattaat tgatgccaaa tattgcatcc actctaattt
     4381 ttgataatct tctttttgtg cgaatttttc tgccttttgt tgaaaaaaaa tgtttgttga
     4441 ccactttaga acatctagtt gtacaattaa cattgctacc ggtattgcta aagttattcc
     4501 tatagaagca tcctggttag ttgagattgc taaataagta ccgatcaatg ctgctgtttg
     4561 ataatccggt actgatgcac caccaaaact agtaatacct agtgtcatta attgcaaagt
     4621 cccaccgata taaagtccag tttgaatatc tcccataata agtccggtca agaaacctgc
     4681 aacgacaggt tgggaggttc cgattttggg cccttcctta tcccagttaa tgaaaaatcc
     4741 atacaacaca actaaaaatg tttgccaaaa cgccattttg ttctctcctt tcttcttata
     4801 cttttatata agcaattact gtgccaacta acattatcca ataaagcggg aaaatacagt
     4861 aaatattaat taaatgagcg tctaaatata ctttggacac cctttcattt ttttagacgc
     4921 tagtatgcta tttagaaaat agtctgctca aaaattgtat agattaactc atactctcct
     4981 ttgggtattt caatacagta aatatctttg atttctgaaa aaaaatcttt tgatacattt
     5041 aaaaaagtat caaatagttg cgctccctct ttcaaatgtt catttgttat tccatcacca
     5101 atcataattc gttcaatcat tccagaaaga tgtagaaata gattaattct cagaaaactt
     5161 gggattttga catcgtaata tttttcatac aaatatagta ctttttctat ctcattgaat
     5221 acttttttgg aatctaaaaa gcgaagctta gtactagcgc cttcaatagt aaagagttta
     5281 actaattcat ttaagcagaa ctcacagcta tccgagtcca tatacttatt taatttttct
     5341 aaggtttgat tcccattaat tagattttct aaatttaaac aatcgattcc ttgaatgtaa
     5401 aattcagtgg t
//
LOCUS       DAFFSV010000149         5140 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFFSV010000149 DAFFSV010000000
VERSION     DAFFSV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.150"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(266..1114)
                     /locus_tag="LR110_002610"
     CDS             complement(266..1114)
                     /locus_tag="LR110_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL4823537.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1113..2180
                     /gene="gnd"
                     /locus_tag="LR110_002611"
     CDS             1113..2180
                     /gene="gnd"
                     /locus_tag="LR110_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBL4823538.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2182..3183
                     /locus_tag="LR110_002612"
     CDS             2182..3183
                     /locus_tag="LR110_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBL4823539.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3180..3464
                     /locus_tag="LR110_002613"
     CDS             3180..3464
                     /locus_tag="LR110_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBL4823540.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3497..4294
                     /locus_tag="LR110_002614"
     CDS             3497..4294
                     /locus_tag="LR110_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL4823541.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4308..>5140
                     /locus_tag="LR110_002615"
     CDS             4308..>5140
                     /locus_tag="LR110_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4823542.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
BASE COUNT         1654 a          838 c         1101 g         1547 t
ORIGIN      
        1 tttaattttt tagaaaataa gaaacaaaaa taataaaaaa aagaaaagat aaatttttta
       61 ctactgtata ctaactttgt tatcgcatct aacaagtaac tcgaataata ccaatacaac
      121 cttctccaaa gatgcgatga catacgttct aacccaccga ctattgatgt tctgtaccac
      181 ccttgcagaa ccaaaaaagc cactaccaaa tatttggtag tggctttttt tgttttatgg
      241 atttttcgct ttttctgtta tggtgctatt ctttgatttc agaaatagta tgtcgaactt
      301 tagcgataga acgacgtgat tctttttcgt tgtaaaacat caaaatcaca tatagagagt
      361 ccaaaatgct tagttgagaa atacgggaag ctaaagcttc agatcggaat tctgtttctt
      421 cagaaataga aataaaaacg acatcgccaa gtttagcaag ggaagaatta gcttggctag
      481 taataacgat tgtttttgct ccagtttttc ttacggtttc agcaatatgg attgtttctt
      541 ttgattgccc tgtgtgagaa atcaaaacag cacagtcttt ttccgtcaat aaagaagctt
      601 ccatcaattg aatatggtaa tccgtgctgt gagaagttgc gataggggaa cgcaaaaatt
      661 tatgataagc gtcagttgct acaatctctg aaccgccaac accgaaaaaa tagacacgtt
      721 tagattcatt gataatagta gccgcttttt ttagatcctt ttgttgtaac aacttttttg
      781 tgtcatttaa agttgaaata ctagactcga acaccttttg cgccatagtg agctcagtat
      841 ctgttttctg aatattttcg tggatagaaa tagcagatag atcattttct tggatcaaca
      901 tggccatttt aaaatcttta aatccattat aaccaagttt ttttgtaaat tgaaaaaaag
      961 tagagtcagc aatgcccagt tcacttgcta aattgctaat aggactatga cttactttgc
     1021 ttggattttc taaaatataa tcggcgatag cctgttcttt tgggctgaag tcgttgtaca
     1081 ttgttttgat ttgtagtctt atggagacgc tcatgagaat tcctcatttt cataataaga
     1141 ttatcatttt ttccgttctc attaatgtta aagatacaag gagagtagga aaaaatcaaa
     1201 caaaaaatat ttttgtaaaa tctatttaca aaaaaatttt tttgtataat gagggtataa
     1261 gaaatggagg cgctatcatg gaaattggtg taattggttt gggaaaaatg ggattgaatt
     1321 tagctttaaa cttaaaagag caccatcaca aagtacaagg actcgacatt tcaagtacag
     1381 ctgttagtca ggcaagagaa aatgacattc ctgcttatca agagatctct gaatttctag
     1441 ctacttttga gcagaaaaaa atcatctggt taatgcttcc tgctggagag ataaccgaaa
     1501 acatgttaag aaaacttttt ccaaaactcg gaccaggaga cattattatt gaaggcggaa
     1561 actcttatta taaagattca atccgtcgag cagaagagtt tcagaaaaat gaaatcggtt
     1621 acttggattg tgggacttct ggaggaatcg aaggagcacg acatcaagcc tgcttaatga
     1681 ttggaggaga taaagaaact tttctatccg tggaacaatt attcaaagat gtagcaattg
     1741 aaagaggata tctgtatgct ggtgcatccg gaagcggaca cttcttgaaa atgatccata
     1801 atggaatcga atatggaatg atgcaagcaa tcggtgaagg attccaactt gtacaaaaaa
     1861 gtgagtatgc ttttgacttg gaacaagtcg cacgtgtttg gaatcatggt tcggtgattc
     1921 gcggctggct gatggaaatc atggaagatc agttaaacga acatcccaat ttaagtaatt
     1981 atcggggaat cgtttctgca tcaggtgaag caaaatggac ggttgaaaca gcgctagaaa
     2041 tggctgttcc agttccagta attgcaacta gtttgttcat gaggaatctt tcacaggaag
     2101 aagatagttt ttcagcaaaa gtagtttcag cactaagaaa tgggtttggc ggacatgaaa
     2161 tagttgaaaa gggtgagtaa aatgccagat tatcatatag caatcgttaa ttccagcagt
     2221 ttcggaagag tctttccaca ccacttggaa agattaaagt cagttggaga cgttcatttc
     2281 tttactgttg atcaagagat tccaggaaaa gaactagcgg agttacttaa aggattcaat
     2341 atcattattg caagtgttac gccatttttt cgacgagatt tttttgaaaa caaagatgaa
     2401 ctgctattga tttcccgaca tggtattggt tacaacaata ttgatttaaa agcagcaaaa
     2461 gaacaccaga caatcgtatc tatcatacct gctttagttg aacgaaatgc tgtagcagaa
     2521 aataatataa ctaatttgtt gtccatgctt cgacgcacga cacaagcaca agcaagagtc
     2581 aaggacgatc gatgggaaga tcgggcggag ttcgtcggta ggacgctgtt taacaaaaca
     2641 gttggtgtga ttggtgcagg aaataccgga agcggagtaa tcgaaatttt gcgtaacggt
     2701 tttcgctgtg atgttctagc ttatgatcct tacaaatcag atttatacat cgaaagcttt
     2761 ggtgcaaaaa aagtagaact tcaagaatta ctggaacaat cagatatcat ttgtttatgt
     2821 gcgaatttga cagaagagaa ctatcacatg atttctgata aagaagtcga tcagatgaaa
     2881 gaaaatgttt atctatctaa tagtgcaaga ggcgctctag ttgatgaaga agcgatgatt
     2941 aatggtctga aaagcggaaa gatcgcagga tatgccacag atgttttaga agaagaaccg
     3001 ggacgtcaag atcatccgta tttagcgttt gaacaagtag tgattacgcc acatatatcg
     3061 gcctacacaa gggaatgttt agaagcaatg ggtgaaaaat gtgtcagaga tgtcgaacag
     3121 atcagtcagc accttcttcc tgaacgttct gtccagacaa agagcaacta tataaaagga
     3181 tgatgaagaa aatggtgaac ggtttaaaag tgaaaacagg ccctcaattt tatttatatg
     3241 aagaaggtgg tatttcaaaa gtttctgatc tcttgaaatc atacggcgcg aaaagagtac
     3301 tggtcaccca cggaacggta tcatgggaaa aggcgctacc taaattagta tttttgaatg
     3361 acgaaacgat tcaatttttt tatcatcgtt attctggaga atgtagttat gctgaagcca
     3421 gacgaatagc tacaatcatc aaaaaaatga aatcgatttc gtagttggcg ttggcggagg
     3481 aaaactgacc gatctagtgg gttatagtgc caatcttgca agaatcgatt tcggagtcat
     3541 ccctacttta gctagtaatt gtgctccatg gacacctctt gctgtcatgt ataaggagaa
     3601 tggacaagca gaagggaaaa cagaacactt ttacagacaa gctgcttttt ttataacaga
     3661 tccccaatta gtgattgatt cccctgttcc atatttcatt gccgggttag cagatacact
     3721 agcaaaatgg tatgaatctg agctcatctt agaacaagat tttttgcaag atgaatcatt
     3781 tttgaagcta gcacaagata ctgcgaagat ctgcaaagaa gagattttga atcattctgc
     3841 taaagcaatc caagatatgg cacaaagaaa actgacatcg gagttcaaac agctttcaga
     3901 gattatcttt gctgttgctg gtttagttgg aggattagga gataaatacg caagaaatgt
     3961 ggcagcacat gctatgcatg acgctatcag taaatatatt ccggaaagcc atcgttttct
     4021 acatggagaa aaagtagcgt atgggatttt ctatcaactg gcactagaag gtcgctggga
     4081 aaaaatagac gagttatatc ctttgtatcg ggaattagaa ctgccggttt ctctagaaca
     4141 aatgggatta tttccgaaag ataaacagat aatagataaa atggctgaat ttattgattc
     4201 gaaagagaaa gtgcatttga ttcctataga ggtaacacag aaatcactga tacagtcaat
     4261 gatggaactg gaaggttata tcagaaagat ctgaaggagg aggacacatg gaaacattaa
     4321 gtgtagtaca aagtttgttg attgctttat gggtagccgc ggttatgtcc cgctggttag
     4381 gtggaggagc cacgttgacc ttacgttttt ctcctttgat gactggatta gtcgtcgggt
     4441 tgatcatggg ggaagttgca caagcgatga ttgttacagc cgctttgcaa atgatttata
     4501 tgggggtttt ttctccagga ggatcaatgc cagctgaacc atctattgca gcagctatcg
     4561 cagtaccagt cgcattatta ggggatctgt cgccagaagc agcgattgcc gttgcggtgc
     4621 cagtcggatt gctcggaagt tacttgtatc agtttcgttt tttcatcaat acttttttag
     4681 gtaaatatac agacaaagct gtagaagatt tgaatacaaa agctatggcc cgatcaatta
     4741 ttctttatcc aacggttgcc tcatttttat tattcgttcc gcttgttttc attgctttat
     4801 atttaggggc gccattgatc gctgatgtaa tcaaagcttt agagggaaca gtcgttatcc
     4861 acattcttga agtagtaggt ggagggttag cggcaatcgg tatcgcgaca actgtatacg
     4921 ttatcggtcg aaaagattat ctggtctttt tcttccttgc ttattttatg agtgttgttt
     4981 tgaaatcttt agatattacg atggttactt atgccatttt tgggattatt atcgctttga
     5041 tctttgtcca gtcgcaaaaa gggaaacaag tacagcctgc cacaagcaat aacaattcag
     5101 catcttttga tgacgaagat gactatgatg acggatttta
//
LOCUS       DAFFSV010000150         5111 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFFSV010000150 DAFFSV010000000
VERSION     DAFFSV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.151"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(368..1342)
                     /gene="sgrA"
                     /locus_tag="LR110_002616"
     CDS             complement(368..1342)
                     /gene="sgrA"
                     /locus_tag="LR110_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBL4823543.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1538..2377)
                     /locus_tag="LR110_002617"
     CDS             complement(1538..2377)
                     /locus_tag="LR110_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL4823544.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2562..3449
                     /locus_tag="LR110_002618"
     CDS             2562..3449
                     /locus_tag="LR110_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL4823545.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4033..4728)
                     /locus_tag="LR110_002619"
     CDS             complement(4033..4728)
                     /locus_tag="LR110_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBL4823546.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4712..5110)
                     /locus_tag="LR110_002620"
     CDS             complement(4712..5110)
                     /locus_tag="LR110_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL4823547.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1625 a         1037 c          751 g         1698 t
ORIGIN      
        1 ctctttcata acaatagttc aaaaattgat atacttgcct ttcattgata tcaataaatt
       61 cttcaaacaa agtacatact cctttttatc tattatttag acttattccg acagctacat
      121 gaataacgta caaatagatt cgttactgat ttttcagaga tactaccatg cgcagtgtgc
      181 attgtcaatt attcttgctc ttattttcca tttgtgttcc tgttatttcc tgtttctcaa
      241 atatgttagt aaacaaaaat agatctggca aagctacaac ttcgccaggc aatacagcac
      301 tctcgaatga aatattcact caaaaatcta tcgttttatt caattttaga tctgttgctt
      361 ttattcttta tttcactcga tggaagagag aacgaaaata gcttgctcct acaatcaaaa
      421 tgagtaacac acccatccat gtaattagct tgttcaaggt tctactacca gtttctggta
      481 aatcgctttt tctattatct gtttctttcg ctttttcttg atgatcaatt ccacgagaaa
      541 caaaatcagg ctgttttagc acacttttta ggttgctccc tgcatttctc ttttgttcct
      601 tagttggtat gataggtacc aaatcaagtt cattatttac ttttcctgtg ctgctttcgc
      661 tgctcgtgct tcctgtgcta ctctcactac tcatgcttcc tgtgctactt tcactactcg
      721 tactctccgt actgctttcg ctgctcgtgc ttcctgtgct actttcacta ctcgtactct
      781 ccgtactgct ttcgctgctc gtgttttctg tgctactttc actgctcgta ctctctgtac
      841 tgctttcacc gctcgtgttt tctgtactgc tttcactact tgtgctatcc gtactgcttg
      901 gcggtccaac aactgtccaa ctgcttggtt tcaaattctc cgtgatcgtc atctgtccat
      961 cataaaaaac aatcaaatca actttatctt tactcttgat ctcatcaatc gatagttctg
     1021 cagaaatcat tattgtatac tcaataccag cggtccattg tacgtctttt aaataaactt
     1081 ccccaataac tttttctgaa cttccattac catcccaacc atatgttaga acagcatgta
     1141 catcactagc attcacgctt ttgtttggtt tgactggaat agtaatgact tgttgtgtca
     1201 tgtcataaac aatccgatca gttttcacag taaatgaaag attctcattt gcccgttcat
     1261 ttgcagaagt actgattcca atcattgctg caattatact cagcccaaga ataacaaata
     1321 atacggtcgc tgttttcttc atttctatca ctctccatat aattaccttc tatttgataa
     1381 aggaattata taaaatgaaa atgatgagaa cacgacagtc agttgtgaaa atctaacaaa
     1441 agatgtcgtg tattcgcttg aatttgttta ctttttcgtt taatcaatga cagacttaaa
     1501 aaagcaagac cgatatcagc cttgctttct tcatctttta ttgataaaca gcagtgtatt
     1561 tcttcatccg ctttgtttta tatgcataga gtaacaaacc aacaatcaag actaacaaga
     1621 ataatgtagt tgatatagat gcttcagccc caaagtttcc cccagtaatt attgtatttt
     1681 gcgattggat cagagtggag aaaaaacctt gttcttgcgt tccgctgact ggtataccca
     1741 aaatagctcc ttgtgtccaa ttccaagtaa tatgagcacc aatagccgta aataaacttc
     1801 ctgtactgta gataagtgcg ctgaaaaagc aacctgctaa aaagatattg aaaactgcca
     1861 gaaatgtcat gttaggattc aatccgtgca aaagagcaaa taatgcagca gatccaatca
     1921 ttgctattaa cggaccaaat ctaagcatct gatgctgaaa aaagccacgg aataaaagct
     1981 cttctgatgc ccccactgca ataaataaaa taaaactcgt tatcagcggc acaatgtttg
     2041 cgtttcctga atctaaccga tacgcccccg tcaaatataa tggactagta ataagcagaa
     2101 acactccaat tccaacaacg agacctcccg ctaaataccc ccagtctttt cctcgaacag
     2161 aaacaccata atcttctatt ctttttcctt cgaccttact agcaagaaat aaagctagta
     2221 aaaaaacagc taaatgtccg accgtaccgg caattgctcc aatccattca ctatttccaa
     2281 taaacatatc cgcaagtgca ttattgacaa gaattcctaa aacaaaacca actaagacta
     2341 ttaaaacaga tccaatcaac cgaataatcc tgatcatctt tcatccctcc tttatattaa
     2401 gtttaacaac ccttttcttt cttgtctcgt gaaaactatg taaatgttct ccatcataga
     2461 gagaatagat ttttacgaac taagaatatc tacagcaacc ttacgaaaat gaaagactgt
     2521 ctccctaatt cttctgttag actgttttta ataaggaaag gatgggaaat tttttgaaaa
     2581 aacaactact aattatgtgc ctttcagcat gtgcaatttt actattctca ggttgtagca
     2641 agtccaatga aacgattgca acgtttaaag gtggaacact tacagttcag gatttttatg
     2701 ataacccaaa agttaaatca aataatcaaa cactattaaa aaaaatgatt gtcttcaaag
     2761 catttgatga tgtatacgga aaagagattt cttcaaagga agtaacaaaa gaatataatg
     2821 aacaaatcaa aaaactcggt ccaaactaca aggaacaatt aaaagcagtt ggccaaacag
     2881 aagagacata taagaaactc tttaaacaga tgctggcttt ccaatacggt ttgaaagcca
     2941 atgtaaaatt aacagataaa gatctagaca ctgcttggaa agaattctat ccagaagtta
     3001 gtacacagat cattctattt tcgactaaag aagaagcaga taaagcaaaa aaagaagcaa
     3061 atgaagggga aaatttctcc aagcttgttc aagcttatgg aaaaaataaa acattaaagg
     3121 aaactgatgg aaaaatgaat tttgattcaa ccaatccaga gattcctact gaagtaaaaa
     3181 aagcagcatt taaactaaaa aacggggaag tttctgatat cattcctgta acagatccaa
     3241 cgacttatca gcaaagctat tatctagtaa aaatggtaaa aaaacaggat aaaggttcta
     3301 ataaagacaa atacaaatct gagctagaaa aaattgctac cgaagcaaaa actcttgata
     3361 cggaattcat ggataaaaca atccgtaaag tgatgaaaaa agacaatgta actattaaag
     3421 atccttatgt aaaaaatata ttcaaataaa caaatttgac ccaattgggg atgtggcaaa
     3481 atttgagaag tgatttttgg aaattccttc ttatttttga ggcaaaccac ttttgccaca
     3541 acttcatcgg ggcctttttt tatttgtacc aaaacaaatc actggatttg ttacaatcat
     3601 gaggatataa tttaaaaaag ttttttattt ctatcacatg aatcaatgcg ccattaagta
     3661 gattctttca tgcttcccta ttttttcact tgccgtatcg cttcaaaaac aactatcata
     3721 aactcaaact cgactgaaaa gttattttaa aaaagactaa aaatatccct tctggacttg
     3781 ttcctcttat tctgttatac tagagaaaca cgaaagatat ttttatgcga cacttttcat
     3841 ttgtttttat gaaatttatt caaaactggc gtaacttcag agttacaagg atgtattaga
     3901 ggttagggaa tacatcgttt aaatgagaaa taagtaatag tcacccaaac tttaaattaa
     3961 tgaatattga atcgtcagat agcctcctcg agttttcgag aaggctatcg tttattttat
     4021 ggagaaattg attcagaagt aatcagcgac cttttttagt tcaccaatcg tttctgcttg
     4081 gaaagtttgc acccaatctt cttggaagcc cctcactgct ttatcaatcg agggcatctc
     4141 taatacttga tctagtagct ctttacctaa cgttatcgca tgagctccag atcgaatggc
     4201 tgtagtgact tgttcacaat ttttgaaact agctgctaat atttttgttg gtctttgcgt
     4261 acgttcgatc tcagtccgca acgaatcaat cactttcata gcatcaatgc cttgattttg
     4321 catcctgtta taataaggtg caatataatc tgctccatta ttaatagcta aatacccttg
     4381 taattctgtg taaatagctg taccggtaat caaataacct tcttttttca actttttgat
     4441 cgcttgaagg cctgcttgat tcaccggcac cttgataaac gtatcttttc ccagttcttc
     4501 tatgataact cgagcatcct ctatcatttc ttcttccgtt cttcctattg tctgaatatg
     4561 gataggaatc tgtccgatta tttttttgat ttctttcata tgcgagaaga agtttgacgc
     4621 tccttctttt ttcagaattg taggatttgt tgttacgcct gttaaattca aaaactgttg
     4681 acaagaataa atactttttg tatttgctgt atcaagcatg atttccatat agctttcccc
     4741 cattctgatc ctcttgatcg attttaatga ttgtcccctt tcttaaatgc ggcatttcct
     4801 tatcttctac atacattgtt cctggaagtt ccggctcatg agatccatca aattttatcg
     4861 tgatatgtcc taattcattc aaattagttt ggacaacatc tcctactgct gtgattacat
     4921 acatttgttt atcaaaacag agcttcatat cacaagttat ttcttttctt acctgattca
     4981 attcaatctt ataacaataa tctgttaatg tttccggtgc ttcatctcca aataaaatca
     5041 ttattttttc tttcctgaat acttctgctt ctataccaac atctgttaca accgtttcaa
     5101 agatcgtcat c
//
LOCUS       DAFFSV010000151         4999 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFFSV010000151 DAFFSV010000000
VERSION     DAFFSV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.152"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            55..534
                     /locus_tag="LR110_002621"
     CDS             55..534
                     /locus_tag="LR110_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBL4823548.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            751..1842
                     /gene="phnW"
                     /locus_tag="LR110_002622"
     CDS             751..1842
                     /gene="phnW"
                     /locus_tag="LR110_002622"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBL4823549.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1835..1963
                     /locus_tag="LR110_002623"
     CDS             1835..1963
                     /locus_tag="LR110_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL4823550.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            1967..2512
                     /locus_tag="LR110_002624"
     CDS             1967..2512
                     /locus_tag="LR110_002624"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL4823551.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(2966..4657)
                     /locus_tag="LR110_002625"
     CDS             complement(2966..4657)
                     /locus_tag="LR110_002625"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBL4823552.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT         1514 a          978 c          947 g         1560 t
ORIGIN      
        1 tccttctttt ttttgttatg atacatgtga aatctttcgt aaaggagaaa gaaaatgcgc
       61 aaagctcaaa gacatttgct gattaaacaa gtcattgaag aattcccgat ccgtacgcaa
      121 gaagaactat taaacaaact tgggcattat ggcgttaccg ccacgcaggc aacgatttct
      181 cgagatatcc gtgatctaaa aatcgtgaag gcacctgatg aaaatggcgt ttcccgcttc
      241 gtattgtttc gcgaaaaagg aactcctgag actaggactg aggaagagga tcggctggtt
      301 caaatgattg aagacattgt attgaaagtc gaacgagtcc actttttgac gatcgtcaat
      361 accttgccag acaatgctca tttatttgca tctgttttag atgagatcaa acctccctct
      421 attgtcagta caattgctgg attcgatacg atcatggtca tctcggaaaa tgaggaggac
      481 gcaaaacgag tagaagaatt tcttcaccat ccaactgaag tatcactttt ttaatcagag
      541 cacgtattcc taatatggaa tgcgtgtttt tttattttca atattttcct tattgaataa
      601 acctttacac tctttacaaa aatacatcat tttttattca aatttttcaa caccttgatt
      661 gaatcacaat aatcatcatt tatattggat gttaacaaga gcagcttctt gctctttgtg
      721 tatttcaaaa aagaaatgaa aggaaatcat atgtataaat tattaacttc tggaccattg
      781 acgacttctg aaacagtgaa aaatgctatg ctgatcgatc attgtacttg ggatcaagaa
      841 tacaaagaaa ttacacaatg gataagaaaa aaattattgg atcttgctca agtatcggaa
      901 ggaatataca caacggtctt aatgcaaggt agcagctcct tcggggtaga atcggtatta
      961 agttctgtga tcaggccaga agatactgta ctgatttgtt caaacggcgc atatagacaa
     1021 cgaatgatca acatgtgcga atgtctgaaa ctgaactata ccgtttatga ggtatcggaa
     1081 catcaaatcc tccaagctca tgtgattaca cagctgattg aagagaatcc gtctatctcc
     1141 gcactagcaa tggtccatag cgagactact tccggtattt tgaataattt ggaagagatt
     1201 gctgctgtca cacaagaaag agatcttttg tttatagtgg acgcgatgtc aagctttggt
     1261 gggataccga tttgtgtagc cgaactaggg attgattttt tgatttccag ctcaaacaaa
     1321 tgtattcaag gtgttccagg cttttctttt gtacttgcta ataaagcgga actggaaaaa
     1381 cgaaaaaatg ttagccgaag tttgtcactt gatctatatg accagtaccg cgtgatggaa
     1441 aacgagaatg gcaaatggcg tttcacttcc cctacccata cggttttggc ttttcaacaa
     1501 gcgctgaagg aactagaggc agaaggagga atcgaaactc gatattcacg ttatctcgcc
     1561 aataatcatg tgctacgaca aaaaatgaca gagatggggt tccagccttt cattcaagaa
     1621 tatcaggggc cattcatcac ctcttttctt taccctaagg actggcgatt tgattttaat
     1681 catttttatc agtatttaaa gcaaaatgga tttgtgatct atcccgggaa aatcagcgat
     1741 catgaatgtt ttcgtatcgg aaatattggc gagatctatc cggaagatat tttggaactg
     1801 acagcaatta tcaaagacta taaggagaac gttaatgatt aaaactgtaa tttttgattg
     1861 ggcaggaaca actgtggact ttggctgtat ggctcctgtc cacgctttta gaaatgcttt
     1921 tctagaaaaa ggaacccaac tgactgacaa agaaatccgc taaccgatgg gaaaattaaa
     1981 atgggatcat atccaacagc ttctttccct cccttctgtc aaaaaacaat ggatagctat
     2041 ttatggacaa ctacctcagg aaaaagaggt ggaggaattg tatcaacaat ttgaacatta
     2101 tctgtttaaa gatttggtca agcattctac cttgaaaccc gacacattgg aaacgatcga
     2161 acggttgaga aacgctggga tcaatattgg ttccacgaat ggatttaatg ctcctatgat
     2221 gacaatcgta gcagaaaccg caaaaaaaga aaggtattca ccagacttca tggcaacttt
     2281 tgaagacgtc gaagggtatg gtcgaccgtt tccttatatg atccataaaa acatgcagta
     2341 cttcaaaaac aagtcggttg atgaagtaat caaagtcgga gatacggtct cagatatcca
     2401 ggaagggaaa aatgccggag tgttgacagt aggtgtaatt gaaggtagtt cgctaatggg
     2461 gatttcttat gatgagtacc aaaaattaga gatggggaaa aagatcgttt aaaacagtct
     2521 gttgctctta gattccaaca agccggcgct gatatttgtg taaacaattt aaaagagtta
     2581 acagaataca ttttgtacgc tgacaagaat ccagaatgac ttttatacaa aataagaggt
     2641 tgtgaaaaag ctgtcctgta tcaagtaata aaaacagcca agcaaaaata gctcctcata
     2701 tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacaccg tttattcgaa
     2761 aaaaaaaagg gagctgtgac acgacttttg tcacagtccc cttatttgca aacaaacagt
     2821 gtttaagaga caacctctcc actaaacggt gttcattcag ctgactctcg gtatttgctc
     2881 aaaaaacgat aaaatttgaa aagctatttt ctcttttgtt tctctaatac tcggatcaga
     2941 acgctgattt cacattctct ttttcttaca tcttatctgg tgcatgaagt cctagcaaac
     3001 gaaggtcttc tttcaataat acagtcactg cataaactag agctaagcgt gcttcttttt
     3061 gttcatcatc agctaaaatc ttcgtatgag catagtattt gttgaatgct tgtgctaatt
     3121 tgattgcatg tttcgcaatc actgacggtt cgtatttttc cccagcagac aagaccgttt
     3181 cagggtattt ttgaactaat ttaaccactt cccagctggt atcgtcattc aatgcatagt
     3241 ctgctgtttc tgaagggaca aatcctgctt tttccaacag gctgactgca cgagcatgtg
     3301 tatattgcac ataaggtcca gtttctcctt caaatcgaac aacttcttcc aggttaaagt
     3361 caaatgtatt cagtcgatcg tttttcaaat cgtggaaaat gactgcccca accccaactt
     3421 gtttagctac agcatctttg ttttccaaat cagggttttt ctcgctgatt tgttctttag
     3481 cagattggat tgcttcattt aatacttctt ctaataagac gatctttcct ttacgagttg
     3541 ataatttttt accaccttgc gtgatcaaac caaatggaat atggtgcatg tcgtcagacc
     3601 agtcaaagcc catttccttc aagacagctt tcaactgttt aaaatgatag ctttgttcat
     3661 tccctactac atatagagat tgtttgaagt cgtaagtacg tttacggtac aatgcagcag
     3721 ctaagtcacg tgtaatgtat agtgtcgcac cgtctgactt cttaatcaac gctggattca
     3781 ggtcataagc agataggtca acgatctctg cacctttatc ctcgttcagc aaatgttttt
     3841 cttctaaaag tttgacgatt tcatccatct tgtcattgta gaaagcttca ccgttcaacg
     3901 aatcgaaacg tacttccagc agatcataga ttttattgaa ttctttcatc gattcatcac
     3961 ggaaccattg ccataattga atagcctctt tatcgccttc ttccagacgt ttgaaccaag
     4021 cacgagcttc ttcatttaat tctggttctg tttcagctac ttcatggaat tggacgtata
     4081 aacgaagcaa ttcattgatt ggttctgctt taacagcttc ttctgtaccc cattttttgt
     4141 aagcgacgat cagttttcca aactgtgttc cccagtcacc taaatggttg atgcggattg
     4201 gctgataacc gattttttcc ataatgaaac cgatcgaatt accgataact gtcgaacgca
     4261 aatgtcccat tgagattggt ttggcaatat taggagaaga catgtcgatt ggtactgttc
     4321 cttgattccc gatgttgcta tccccataat gttctttttc tttcactact gtttgcaaaa
     4381 cttttttgct gataagttct ttattcatga aaaagttcaa gtaaggtccg acaacttcga
     4441 ttttttcaaa gttggcagaa tcgatttttt ctgctaaatc tgcagcgatt tgctgtggcg
     4501 cttttcggta aacttttgct aatgaaaagg ctggaaatgc aacatctccg tgttcagctg
     4561 attttggatt ttctagtaat tgctcgactt gttccagtgt taaatcatct ttgacagcgt
     4621 catagacagc ttttgcgaca agatctttat tattcatctt tattcctcct aaagtgttcc
     4681 cttgattgtt aaacaaaaaa aatctctagc gacggcatac ttagtatgcc atcactagag
     4741 acgagatttc ccgcggtacc actccaattg tcctaaaaaa ggaccagctc catctgatat
     4801 cggtagacga ccgtctgttt aaaacagaat tctccaagtg cgcttcgttg tatcttcttc
     4861 attcggcttc caccacttcc gaattcgcta gccaagaatg atacaagtac tctcttgttc
     4921 aacaaatctt agtcttatta aactttcttg aattatatca gaaaaaaaaa cgaaaggcta
     4981 gtcgtttcat aaaaaaaaa
//
LOCUS       DAFFSV010000152         4983 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFFSV010000152 DAFFSV010000000
VERSION     DAFFSV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.153"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..394
                     /locus_tag="LR110_002626"
     CDS             <1..394
                     /locus_tag="LR110_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBL4823553.1"
                     /translation="VIKKEQGFTGTETKSLFLKDKKDNNYVYLTFTTKQTDFKALGKI
                     VGKRLSIVSSERMEEQTGQKQGAVSPFGYEKQIPIIIDEELLSNEKLVFAPGRPDQTM
                     VIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            431..1441
                     /locus_tag="LR110_002627"
     CDS             431..1441
                     /locus_tag="LR110_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBL4823554.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1556..2269)
                     /locus_tag="LR110_002628"
     CDS             complement(1556..2269)
                     /locus_tag="LR110_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823555.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2459..3049)
                     /locus_tag="LR110_002629"
     CDS             complement(2459..3049)
                     /locus_tag="LR110_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBL4823556.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3533..3760
                     /locus_tag="LR110_002630"
     CDS             3533..3760
                     /locus_tag="LR110_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBL4823557.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3776..4942
                     /locus_tag="LR110_002631"
     CDS             3776..4942
                     /locus_tag="LR110_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBL4823558.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
BASE COUNT         1684 a          818 c          998 g         1483 t
ORIGIN      
        1 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
       61 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
      121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
      181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
      241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
      301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
      361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
      421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
      481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
      541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
      601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
      661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
      721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
      781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
      841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
      901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
      961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
     1021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
     1081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
     1141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
     1201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
     1261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
     1321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
     1381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
     1441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
     1501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
     1561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
     1621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
     1681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
     1741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
     1801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
     1861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
     1921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
     1981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
     2041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
     2101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
     2161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
     2221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
     2281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
     2341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
     2401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
     2461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
     2521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
     2581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
     2641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
     2701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
     2761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
     2821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
     2881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
     2941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
     3001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
     3061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
     3121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
     3181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
     3241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
     3301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
     3361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
     3421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
     3481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
     3541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
     3601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
     3661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
     3721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
     3781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
     3841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
     3901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
     3961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
     4021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
     4081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
     4141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
     4201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
     4261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
     4321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
     4381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
     4441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
     4501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
     4561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
     4621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
     4681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
     4741 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
     4801 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
     4861 tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga
     4921 gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa
     4981 aaa
//
LOCUS       DAFFSV010000153         4940 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFFSV010000153 DAFFSV010000000
VERSION     DAFFSV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.154"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..182)
                     /locus_tag="LR110_002632"
     CDS             complement(<1..182)
                     /locus_tag="LR110_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL4823559.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="LR110_002633"
     CDS             517..951
                     /locus_tag="LR110_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL4823560.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="LR110_002634"
     CDS             974..1294
                     /locus_tag="LR110_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823561.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="LR110_002635"
     CDS             1435..1626
                     /locus_tag="LR110_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823562.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="LR110_002636"
     CDS             complement(1697..1954)
                     /locus_tag="LR110_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBL4823563.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="LR110_002637"
     CDS             2112..2375
                     /locus_tag="LR110_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823564.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="LR110_002638"
     CDS             2399..2587
                     /locus_tag="LR110_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823565.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="LR110_002639"
     CDS             complement(2670..2855)
                     /locus_tag="LR110_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823566.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="LR110_002640"
     CDS             3070..3441
                     /locus_tag="LR110_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL4823567.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="LR110_002641"
     tRNA            complement(3591..3663)
                     /locus_tag="LR110_002641"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="LR110_002642"
     CDS             complement(3709..4137)
                     /locus_tag="LR110_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823568.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4928)
                     /locus_tag="LR110_002643"
     CDS             complement(4197..4928)
                     /locus_tag="LR110_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL4823569.1"
                     /translation="MFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSSTQKTKKTKEK
                     AEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDYVPVFNADGT
                     HGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLDDIKKALSKG
                     NPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIYVNDPYGVKN
                     RAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1731 a          772 c          973 g         1464 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa
//
LOCUS       DAFFSV010000154         4835 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFFSV010000154 DAFFSV010000000
VERSION     DAFFSV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.155"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            28..327
                     /locus_tag="LR110_002644"
     CDS             28..327
                     /locus_tag="LR110_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823570.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            592..1467
                     /locus_tag="LR110_002645"
     CDS             592..1467
                     /locus_tag="LR110_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823571.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1464..1682
                     /locus_tag="LR110_002646"
     CDS             1464..1682
                     /locus_tag="LR110_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823572.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1669..4296
                     /locus_tag="LR110_002647"
     CDS             1669..4296
                     /locus_tag="LR110_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823573.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4311..4529
                     /locus_tag="LR110_002648"
     CDS             4311..4529
                     /locus_tag="LR110_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823574.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         1885 a          605 c          971 g         1374 t
ORIGIN      
        1 ataattaatt aaaaaggaga ggtttttgtg gaagcaataa aattaatgat ttcagaagaa
       61 caggaggaaa tgtttctaaa gcagttttat caaattgcta caaaagctat tgaaaaggct
      121 caagaaaatg cggggatgac aagggaatat ctcaatcaaa aagaaatggc ggaatactta
      181 aatgtgagtg aaaacttttt aaaaaatgct gtgagaaatg agggattgcc cgtagttgaa
      241 ttaggggcaa gaaaatttta ttcgaaaaag gcagttaatg agtttatgtt tgctcgaatg
      301 aaaagagggg aaaatattgg aagatgatga agtagaaatt ataaaaaaat gctttaaaaa
      361 aagaaaaaaa gtgttcccaa aagtttgcag actgggggaa cactcaataa taaagtgctg
      421 agaaaacttt atttatagct attatatcaa aaaagaaggt gaaaaggtgt tttcttctgg
      481 aaatgttgta gttgacaaac ttagagaaat aaattttgac ggaaatgtaa tccacacaca
      541 tggtacgatc aattaagaaa aaaaacgaaa aaaggtgttg agaagcccta tttgaatgca
      601 atagttatct tagcagaaat tacctattgg tatagaccta aaaaaatttt taacgatgat
      661 ggtcagctac ttggttatca aaaaaagttt cttgaagata ttttgcaaaa atcatataag
      721 caattaagca aaaaaacagg attatcagaa cgagctataa aagatgcaat tgtgtactta
      781 gagaaaaagg ggattatcaa aagggttttt agaaatattt attatgagaa aagagcagtt
      841 acattatcaa atgttatgta cattgtttta aatgtacata aattgataga aattactttt
      901 cctactttgg attgcgaaaa aaaggagtat atgccgaaaa ttcgtcagag gggtatcgaa
      961 aaaaagagat atgtctcttt aaaaaaaggt agaggtatgt acaaatttgt atatacgtat
     1021 acaaagacta cttcacagaa catatcaaag actactctcg ttatggaaga agaggatatt
     1081 aatataaaga ggatttacag taagattagt gttctttatg acgcaaataa cgaagatggt
     1141 cgaaaattat ttcaagtgta tcactatttg agaaacgcta atgtagatac agaagatatc
     1201 agaaaaatca ttgaatattt atctgaaaat actcagtatc ttattgctga gtgtattata
     1261 aaacaacatg aagcatgctt ttttcaaaga aaaactgaag aagggttata cgattatgta
     1321 acatattttt tgaatggact tcagaaaaga tataaaagtc aatttttaga aaatgtagag
     1381 cctatagatc agttcaaaaa agctttcgaa atcaaagagt tacctgaaat tactatgcat
     1441 aattggttga aaggagaaga atcatgatta aaaatcgctt aatagatcaa atgtggtgtc
     1501 ttgatcaaaa tacagatact ctcgaattaa tcgagagtat ctgcttcaac acagtagttc
     1561 tcgatttaag agctgaaaat gacaattgtg taactcatat cataattaat caaaaaatgg
     1621 ctcagcagct ctctgagagc ctaagaaaat gggctgaagg agaaaactat gaaagcaatt
     1681 aaagaaaaaa tcgttagtag aaaaataaac tggttagcac tcagtgtact tgttttaatg
     1741 attagcgttg tttttacaag taatattgtt gaagctagca ttttcaattg gtttgatgac
     1801 acaaaaagtc aaacagagtt tttgaataat tctctttata gaccttacct aaaaaaatac
     1861 aatggagatt tccttagtca gttcgggatt tggcttggat gggctgtggt aaaaggcatg
     1921 tttactgtga ctgattcgat tcaaaatatg attccagatg tgttggattt gttcaatttc
     1981 attgaaagta cagggctaaa taatgtttat caatcagtca taaatacaat tgtcgttgga
     2041 ttaatgatcc tttcattgat gtttgttggc tacaagatga ttactggaaa aggaacaata
     2101 gatttaagat ctgttggaat gaatatagtg atgtctgtag cgttaattct tttaatgcca
     2161 acaatgattt ctagcggtat tcaatttgcc aaaatatttt acaatgatgc tactacgatt
     2221 acaaattcag atgacggagt cgcttggtca ttgataaaac aaggagtaac agatctggca
     2281 tatattaata aaacagatca atactcaagc attgataaaa cggaggatcg aaataagtta
     2341 acacgaaaaa attttcaaca gacagattta acacaagtgt tgacggataa agtgattgat
     2401 aaattggaaa aagaaaatcc agctgcggat aatcttcgct atgaattagt tgaaaattct
     2461 aataatgaat ttgttgccac aaagttttct gataacttct tatcaacatt ttctgatagc
     2521 ttgaaatcag gttattatcg atatcaagca aatctttggg ggatttcgat tgggttgaca
     2581 gcgttggcaa tcgcctatgt gttcagcgcg tttgtgatca ttacagcaat attagaactt
     2641 gcatttaaac gtgtgttagg tgttcttgtt tttgcgacag atattgaaac aggacaacgt
     2701 tcaaaagttg ttttatccga tattttacaa tgttacttaa cagttggatt tcaagggttt
     2761 ggactatcta tgtttgctat gtttatcaat tttttaaatg ctggacaagg tatctccaca
     2821 aatattttta taaaaacaat tgcttatatc tgtgctgtgt ttgtgctgat aaaaggcagt
     2881 ggaacagtca tgagatactt tggtgttgat attggactca aagaaggcta tgggcaactt
     2941 gcttctgctt ttggtatggg cgcaatgctg tttagaaaag gatcaactgg atttaatcgt
     3001 gctaaaggaa gcggaaatgg aaatggatca gacagtggag aaggagaaga tcgaaaacca
     3061 gaaaagaatt tcggcgaaac tctatcaaaa aaagctggca aaactggaag agctttggga
     3121 tatgcccatg aacgtggatt atcaggttta gcttcagatg gagctacaat ggctagtgaa
     3181 agagcaataa aaccatttaa gagtatgcgt gatatggcta atgataccaa gaataagttc
     3241 aaagaaggtt tagatgatgg tacagtgtcc gctattaata aaaatagcaa acctatgtta
     3301 gctaaaaata aagaagatga aactggaaaa tatgctgatt ccatgccaac acgtctttcg
     3361 gatagaaaag atggtgctaa ggttgaaaat gccgatagaa ttatgtctag ttctgaacgt
     3421 atgcgtgaag caatgaaaaa taatgctgaa tcgaataata atcctgtaag tgcaattcag
     3481 caaaaagttc aacaagatat tgaagaacgt aaaaatgcta tgcacggtca agctaagtct
     3541 gctgaagaat taattaatca gaagcgacaa gaagctaaat acacgcctga agcaatgaat
     3601 cgtgaggaaa tgttaaggaa acgtgtagaa gggcgtacag gagctaatac ggacgaaaaa
     3661 gaagcgttga ccaaagaaag aatccaagaa gctaaaaact caaataccgg attagaaaaa
     3721 ttggtcaaag aaaatctaca aaattctaca agtagtcaag gtggccgatc agtagatgtc
     3781 cgagaaaata ttcaaggttc attgaatggt catgtcggac ggaatgttga tgtaagagag
     3841 aatttacaaa gctcttcaaa tggtcaaggt actaagacaa ttgatgttcg agaaaatgta
     3901 caaaattcta caagtagtca agggtttaaa tcaatggatg taagagagaa tgtacaaaat
     3961 tctacaagta gtcaagggtt taaatcaatg gatgtaagag agaatgtaca aagttctaca
     4021 agtagtcaag gattcaaaac agtagatgtt cgggagaacg ttcaaaaaga tagtggagaa
     4081 atgaaagaaa aaacacaaaa aatcaatatc gttgaagaca gaaagtcagc gaaaaaattt
     4141 gattcaaatc atgaaacagt gattattgat agtgaaatcc gtgaaaatga taagggatca
     4201 aaaccaagac gaagatttac ctatgaagac aacgagttat tcagagatac acttaatgat
     4261 cctaatccgc ttttcgataa tctattaaaa aaataaaagg gggaattgtc atgaaattaa
     4321 ttctaaagat tagtacgcat gatttcttag aacttgacga attatgtgaa tcagtaaaga
     4381 acgcaacaaa tgaaaaaatc acggtcacta attatattga actgatgatt caagaagaat
     4441 taaagatgaa acatgagata aaaaacaact tgaaatattg ttatctagaa aaaagtgaag
     4501 agataatcca tgaattaaat ggcttttaaa aattgtattt gacatgaatt tacgacaagg
     4561 aattagtggg gattaagcga ttattctctt ccactttaga gaaataatgg gttaaaaagc
     4621 cagaatacgt agagcaatat tgacaaccta taaaggagct attgttgaaa attgaagtaa
     4681 caaaaagata aagagaggtt tacatctatc agattaaaag tgatagtaca cccatgaaac
     4741 ctgcaaaaga gttgaaccta atgtatatcg gtttaactct ttttatttgc aaattaaaaa
     4801 aatatcaccg aagtgacgaa tgaaactatg accaa
//
LOCUS       DAFFSV010000155         4824 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFFSV010000155 DAFFSV010000000
VERSION     DAFFSV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.156"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            106..357
                     /locus_tag="LR110_002649"
                     /pseudo
     CDS             106..357
                     /locus_tag="LR110_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="LR110_002650"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="LR110_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL4823575.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="LR110_002651"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="LR110_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="LR110_002652"
     CDS             1786..1869
                     /locus_tag="LR110_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HBL4823576.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="LR110_002653"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="LR110_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL4823577.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="LR110_002654"
     CDS             complement(2994..3857)
                     /locus_tag="LR110_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HBL4823578.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="LR110_002655"
     CDS             complement(3859..4131)
                     /locus_tag="LR110_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL4823579.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="LR110_002656"
     CDS             complement(4148..4357)
                     /locus_tag="LR110_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBL4823580.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..4598)
                     /locus_tag="LR110_002657"
                     /pseudo
     CDS             complement(4455..4598)
                     /locus_tag="LR110_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
     gene            complement(4652..>4824)
                     /locus_tag="LR110_002658"
     CDS             complement(4652..>4824)
                     /locus_tag="LR110_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL4823581.1"
                     /translation="RPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSV
                     CTEIKVLLGIPA"
BASE COUNT         1475 a          819 c          892 g         1638 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgctggt tctgttgcaa agttttaaat
     4621 aaagaataaa atcccttacg gtatctatga tttaagctgg gattcccaat aataccttga
     4681 tttcagtaca gaccgaaaac ccgaagagag tgccttcttt tcgggttttc ttatataatc
     4741 ctcgaatggc ttccatgcct ttaatcgtgg tagaggcagt gcgtaaactt cgatagaatt
     4801 tattgcgtct ctttactgga cgat
//
LOCUS       DAFFSV010000156         4799 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFFSV010000156 DAFFSV010000000
VERSION     DAFFSV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.157"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(44..1192)
                     /gene="nagA"
                     /locus_tag="LR110_002659"
     CDS             complement(44..1192)
                     /gene="nagA"
                     /locus_tag="LR110_002659"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBL4823582.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1268..2104)
                     /locus_tag="LR110_002660"
     CDS             complement(1268..2104)
                     /locus_tag="LR110_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4823583.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2288..3505)
                     /locus_tag="LR110_002661"
     CDS             complement(2288..3505)
                     /locus_tag="LR110_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL4823584.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3654..3833)
                     /locus_tag="LR110_002662"
     CDS             complement(3654..3833)
                     /locus_tag="LR110_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823585.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3888..3971
                     /locus_tag="LR110_002663"
     tRNA            3888..3971
                     /locus_tag="LR110_002663"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3922..3924,aa:Leu,seq:gag)
     gene            complement(4211..4789)
                     /locus_tag="LR110_002664"
     CDS             complement(4211..4789)
                     /locus_tag="LR110_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823586.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1457 a          999 c          775 g         1568 t
ORIGIN      
        1 accaaactta agaagtttag ttttaaggtt aacttaaatt atattatgcg tggaaacgtt
       61 ccacgccatc tagataagtt gcttccaatg tcatatcagg gttcaatacg ataaagtctg
      121 catcgcgtcc ttgagcgatt tttccacatg tatcatcgat tttacagctg attgctggaa
      181 tcagactagc catcatgact gcttgttctg gtgttgctaa tccccagtct acaacatttt
      241 taattgcttc tttcaatttc aatatacttc cagctaagtt gccagattct agacgtgctg
      301 ttccttcttt tactactact ggaaattcgc ctaaattgta atttccatca ggcatcccac
      361 cggccatcat acagtctgta attaatgcga catgatcatg tcctgctttt tccattagga
      421 ttcccgcagc atttggatgt acatgatggc catcacaaat caattcagaa aagacatgtt
      481 ctaaagataa taacgcaccc accatacctg gttcgcgatg attcaatcca cgcataccat
      541 tgaaagcatg gacaaataca cttgcgcctg cttcaacagc tttttgcgct tcttctagtg
      601 tagcattact gtgtcctaga gaaacaacta ctccttcttc agtcacttgt ttgacaaact
      661 cttctacacc ttgacgttcc ggtgctaaag caattttttt gattaaacca cctgaagctt
      721 cttgccattc atggaaagta tccaaatcag gatccccgaa ataacttggg ttttgtgccc
      781 ctttgtgttc ttctgtgaag aaaggacctt caaaatagat tcctttgatt ttagctcctg
      841 gtgcttcctg atacattttg ccgattgtct cagctacatc ttttaaacgt tctttgcttg
      901 acgtcaatgt tgttggtaaa aatgaagtca ctccacaaga taacagacct tcagacatca
      961 ctttgatgcc ttctgcatca ttatccatta catcatgatt catatagccg tgaatatgtg
     1021 tatctaccaa accaggagca atccatttgc cgctttggtc aatgatgttg acttctccct
     1081 caggtagttc tttggtgtat tcaccaaaaa gtccatcggt aatttcaaga tagcctgcat
     1141 gtttgacacc tgacgctaaa aagaatttgt cagcatagat aaaagttctc attctattct
     1201 ctccctttgt ttttctacct ctaaagtact cttatttaga gaagaagtca agaatggtct
     1261 acaccattta caaatttttt gaaatttgtt tcacagcttg tgctaagcct tcatagaatg
     1321 ctgctctttc tggttcttct ttacgcttgt atattaagct tttaagcaca aataaatcat
     1381 tgaggtaata gtagctattc ttctcttgag cccaatagat tccttgattc acctgttcta
     1441 acgcttcgtc gatttcattt tgatggaata agaaatttcc ataattataa aaaatttgcg
     1501 aaaattcact ttttgatcgt tcatcagctg attgatgcag cagttggatg cttcgactca
     1561 aatagtagcg tgcttcagcc gtttttcctg ccacaccata tatttttccg atacaactaa
     1621 tcagttgtat ttctgtgtca gaaacatgga ttctttcttt ttggctagag tagcttaatg
     1681 cttctttcaa ataataaagg gactgagaga tattttgacg caacgtgaat tcgcaaacac
     1741 cgagataata ataatagcgt tgaaagtcag ttgcttgata cagattttca gaaatatcag
     1801 aagattttag tacttgttct aacttccgat aatcacgttt ttggaaacaa ctatccaaca
     1861 gttcaaataa tttatcgttt gtccgagtag tttttcgaga gagattcatc acatgatcaa
     1921 tcgttacccc tagccgttga caaatctgca tcatcaccaa tacattcggc agttcctcat
     1981 tattttcgat ccgacttaat acactttgtg aacaaatatc atgcgaaagc attttttgcg
     2041 tcattttttg ttcttttcga atctctttaa tgatcgttcc aaaggactcg attaacgttt
     2101 ccataagtat cctccccaaa atatgcgtat tcgcattcta ttcgtatttt actttaggaa
     2161 gtcgcaaact tcaagaaaag acttgcaact aaataatata ctttagttat ttaagaaaac
     2221 tccactagaa agagagcctc agtgtgtttg tctgtggccc tcctactgaa aaatttggtt
     2281 tttaaaatca tttttggcga taggttgcta ggaattctct cattttctta gctgtttgtt
     2341 tcaaatcatc cattttagga agataaacga ttcgaaaatg atctggctgg ttccaattaa
     2401 atccaccacc atgtacaagt agaatatgat gttcatgtaa aaagtccaaa acgaattttt
     2461 catcatccaa aatattgaat cgctttgtat caatttttgg gaatatataa aaagcagctt
     2521 ttggttttac ggctgaaaga ccaggaatat cattgattgc attatagata tattctcttt
     2581 gttcatagat ccttccgcct ggcaggagca gctcatcgac actttgataa ccgcctagcg
     2641 cggtttggat aatttgctga gacaatacat tggaacacaa acgcatggag gacaacatat
     2701 tcagcccttc tatatagcct ttgacgtgtg acttatctcc acttaggacc atccagccta
     2761 ctcgaaatcc tgctacacga tgagatttgg atagaccgtt cagcgtaaca acgaacaaat
     2821 caggcgctaa tgtagcaatc ggaatatgtt ctagaccatc catcactaag cgatcataga
     2881 tttcgtcgga ataaataatc aggttatttt gtcgagctac ttcaacgatt tcttctaata
     2941 attcttttgg ataaagtgcc ccagtaggat tattaggatt aatgataaca atagcttttg
     3001 tccgactcgt tatttttgat ttgatatcat caatatcagg ataccattct gcctcttcat
     3061 cgcaaatata atgaacaggt gtgcctccag ctaatgcaat agaagccgtc cacaacggat
     3121 agtccggcat tggaaccagt acttcatctc cattgtccag caacccctgc atacacatcg
     3181 tgatcaattc gctcactccg ttgccggtgt agatatcatt gatcgtcaca ttcggaaaat
     3241 gtttcagttg gcaatattgc tcaattgctt ttctagcaga aaagatcccc ttggaatctg
     3301 agtagccttc tgagtcacgg gcattcatga tcaaatcacg aatgacttcg tttggtgctt
     3361 caaatccaaa aggtgcggga tttcccgtgt tcaattttaa aatactaatg ccctcttctt
     3421 gcatccgctc cgcttcttct agaacgggac cgcgaacatc gtaactgaca ccttctaatt
     3481 tgttggattt ttcaaattct ctcatcactt agccaatccc tcatttcaaa aagttagaac
     3541 tttccaaatt ttttgttatt tactatttca atatcatact ccatcctaga aaattttgcc
     3601 acttatttag gaaaaacgta agtattaaaa aaacacaaac aagcgttgag aacttaattg
     3661 tcatgatagt cattccatag attcgctgcc caatccattg caattccaca aatgattcct
     3721 agcataaaac caagcaaaaa acgtccgaat gcaagtccaa tcaatacacc taagatcatt
     3781 cccagtcctg aaaattctcc gtcatatttc tccatcttaa taagtcctcc catttttgag
     3841 cttgacaact atgtaaaacc tcgctatcat tagtgtgtta ttattacgca gatgtgttgg
     3901 aattggcaga caagcatgat tgagggtcat gtgagcgtta gctcgtgtgg gttcgagtcc
     3961 catcatctgc actacaactc gtgaagttaa ttttgcttca cgagtttttt tgtttgaacg
     4021 tttctttatg ccattatata catatcactg tatcctttac aaaaagaacg tacaatcatg
     4081 taaacagcct gaggtgagta tatgaaggtc ggaaacaagc tactcgtaga aacttgcaag
     4141 tgttaaaaca attaatcgaa aaacgagtaa aatccctcac ctaatactaa agcgagggat
     4201 tttttgtata ttatctgttt atcaccgtta ttataaaggt acaaatacca agcacggtta
     4261 atccaaattg cataaattca tttttttgta cactcatgct tttcattagc tctttaactg
     4321 ccattagtac taagatggcg ataacaacat aaagtatata gtacgtccgc attccggaag
     4381 cccatactgt gggagaaaat cccataatta accttgaaca aaatcccatt atcatcaaaa
     4441 taactaaaaa aatagaagtt ttcttattgt caaaacttaa ataaattcca acaattaaac
     4501 atagtaataa tgccaagact actaaagtag ctatccaagt acctggataa aaaagtgaca
     4561 gtttcgttcc taactctgta aagagattat tcaggttact actactccaa atcattgccc
     4621 ttttatttcc attgacgtaa gtaaagcttt gacccattgt attaccaaaa taaataatca
     4681 ggtttaagaa aaacggtaat gcagtcaata tacgcacata atagttttga catttcctat
     4741 atgttagaag gaaaattaag aaaaatagca ataaaaataa gatattcatg tctaaaaaa
//
LOCUS       DAFFSV010000157         4784 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFFSV010000157 DAFFSV010000000
VERSION     DAFFSV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.158"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            6..863
                     /locus_tag="LR110_002665"
     CDS             6..863
                     /locus_tag="LR110_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL4823587.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            885..1394
                     /locus_tag="LR110_002666"
     CDS             885..1394
                     /locus_tag="LR110_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL4823588.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1431..1832
                     /locus_tag="LR110_002667"
     CDS             1431..1832
                     /locus_tag="LR110_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823589.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1944..2297
                     /locus_tag="LR110_002668"
     CDS             1944..2297
                     /locus_tag="LR110_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823590.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2331..2774
                     /locus_tag="LR110_002669"
     CDS             2331..2774
                     /locus_tag="LR110_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL4823591.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2791..3876
                     /gene="uxuA"
                     /locus_tag="LR110_002670"
     CDS             2791..3876
                     /gene="uxuA"
                     /locus_tag="LR110_002670"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL4823592.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3869..4582
                     /locus_tag="LR110_002671"
     CDS             3869..4582
                     /locus_tag="LR110_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL4823593.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         1658 a          730 c         1081 g         1315 t
ORIGIN      
        1 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
       61 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
      121 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
      181 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
      241 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
      301 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
      361 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
      421 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
      481 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
      541 tcactatggg ccgtaatgct acaggcctaa agcaaaacac tggtgttcag acagcgattg
      601 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
      661 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
      721 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
      781 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
      841 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
      901 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
      961 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     1021 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     1081 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     1141 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     1201 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     1261 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     1321 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     1381 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     1441 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     1501 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     1561 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     1621 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     1681 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     1741 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     1801 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     1861 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     1921 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     1981 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     2041 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     2101 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     2161 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     2221 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     2281 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     2341 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     2401 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     2461 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     2521 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     2581 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     2641 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     2701 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     2761 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     2821 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     2881 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     2941 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     3001 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     3061 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     3121 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     3181 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     3241 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     3301 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     3361 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     3421 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     3481 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     3541 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     3601 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     3661 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     3721 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     3781 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     3841 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     3901 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     3961 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     4021 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     4081 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     4141 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     4201 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     4261 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     4321 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     4381 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     4441 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     4501 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     4561 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     4621 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     4681 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
     4741 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tcca
//
LOCUS       DAFFSV010000158         4766 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFFSV010000158 DAFFSV010000000
VERSION     DAFFSV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.159"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(207..1169)
                     /locus_tag="LR110_002672"
     CDS             complement(207..1169)
                     /locus_tag="LR110_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4823594.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="LR110_002673"
     CDS             complement(1171..2610)
                     /locus_tag="LR110_002673"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBL4823595.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="LR110_002674"
     CDS             2795..4741
                     /locus_tag="LR110_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBL4823596.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1517 a          817 c          788 g         1644 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatgga
//
LOCUS       DAFFSV010000159         4662 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFFSV010000159 DAFFSV010000000
VERSION     DAFFSV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.160"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(218..943)
                     /locus_tag="LR110_002675"
     CDS             complement(218..943)
                     /locus_tag="LR110_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823597.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1097..1423)
                     /locus_tag="LR110_002676"
     CDS             complement(1097..1423)
                     /locus_tag="LR110_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823598.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1460..1825)
                     /locus_tag="LR110_002677"
     CDS             complement(1460..1825)
                     /locus_tag="LR110_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBL4823599.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1862..2077)
                     /locus_tag="LR110_002678"
     CDS             complement(1862..2077)
                     /locus_tag="LR110_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4823600.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2206..2541
                     /locus_tag="LR110_002679"
     CDS             2206..2541
                     /locus_tag="LR110_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4823601.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2617..4614)
                     /locus_tag="LR110_002680"
     CDS             complement(2617..4614)
                     /locus_tag="LR110_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823602.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
BASE COUNT         1458 a          918 c          607 g         1679 t
ORIGIN      
        1 tatttcacta aaatcactac ctgaaacagt ctaaaacact tatatatcaa cgtttaatga
       61 aactatctcc gtttcatttc agatacaaat aaatatagtc gggctccaaa tccgaaaaaa
      121 aataccccca gtagcttatc ctaagctact ggagatgtat aagtacggtc atataacaac
      181 ggggtagggt aaataaatga aaaagatgga agaatcatta ttcaataact tttttaaata
      241 cttcgttgta atcagttacc tgtccgttct taaataccac agaataatct tttttcccat
      301 tttcaagtaa ccgaagtgga atatctttac caactttctc tgtcactgca ttactgatac
      361 ctgttagatt agctaataaa tctgataaag attgagcatc tgtagaaatc tctgtatcag
      421 tatttatcgt gattccttta tcttcattta caagtttcca agtaggtggt atagctgtgt
      481 atgtttctac aaatgaatta agtttttcat taagtgactt ctcttttata tttccatcta
      541 aaaactctgt atatatggct tgagctatac taaaacaaag ctggccttgt tctaataatt
      601 ttacatcatt agttagtaaa gctgtgttca cgttatcaat cgcttcaaga agagtatctt
      661 tggtgtccct aacagcttta ggtgtgcctt ctggaatttc cctacttgat acctcgtcca
      721 ttttatcttc cataaacccc ttaaaatcat atctttctcc caaatccata gaagtttttg
      781 agaaagcttt catttctgag tctatttctt caataatatt taacatgtct gatacatatt
      841 gtttaggctg attcgttttt aatctatatg tggtaaatat ctcaattcct ccaattatca
      901 ataaaattgc caaacaaatc cataataatt ttttcttttt catttttcct cctatacact
      961 gctgttataa cagtgactaa tgtaaagatt ttcccagcaa tcatcagact attatttaca
     1021 gggaatcaaa aaaactcctt agtaagctta cacctttagg agttcctgtt tgaacggtat
     1081 gcataataca tttaaatcaa tttaacatta acacctcagt atatggtatc tctacatata
     1141 cacggtcttt ttttggatag tactcgacat ttatattgat agcacttcca ttgtctaacg
     1201 tagctttagc ccggtacgtg ttatcaccat tatcatgagt gatcgtcaca tcatcacatg
     1261 tccgatttaa gttatactgt tcttgtaaaa tctgagttac caatggtaca gatgacttct
     1321 ctagtccaga tgaacccgtc attcttgaaa aaattatacc tataatagcc aatacaacga
     1381 tacctataat gacaaacttc ttattcatat ttactttatt caacttttta tctcctatct
     1441 ccaaaatgat tttctctcat cagtaactca ttgacatccc caatagtaca ccaaagatgg
     1501 ctccagctac ccctgctatc atcaaaatcg tacccatttg ttcatctttc tctcgatata
     1561 agtaacccat aataacagca acagcaccaa ataccacagg aataaatgct aatgatacta
     1621 ccatagaaat ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt
     1681 gttccttcat ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg
     1741 tctcatctgt ttcttgtaat gctccgcaaa actggcaata cttactttca tttgttattt
     1801 ctttaccaca ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta
     1861 gctaactgtt tactttatta aatacatcag aaaacagctc atttattggt acacttaata
     1921 tctcagaaag cattttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac
     1981 ttacacattg ctgtgtaaca ttcatttttt tggctaactc agtttgggaa agtccttttt
     2041 cttttctgta tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta
     2101 tatacaacaa tatgatgtat ttcaactcat aaaatatata aatacttcaa atgtcattac
     2161 aaatacaaca acttgttgta ctctactgag aaggggggtg tattaatgaa acagaagcaa
     2221 tatgcaaagg tcattagaga aaagcgaaaa gcactcgggt ggacacaaaa agaattagct
     2281 aaaaaaatct tctctacaca acaggcagta gcaagatggg aaaattctgt aacagaacct
     2341 aacctcgata gcttaacagc tctttctaga gccctaggaa cccctgtaag ccattttcta
     2401 gacaatgttg acatagatta tgaggaagag tttttggctc tctatcgctc tcttagtact
     2461 gaagatgcaa ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc
     2521 aatcagctat tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt
     2581 gagatgaaat aagattaaga gctatggata acctgtctag tttaatataa ttgggtctct
     2641 tctattcctt actctccgtc catcatctct tgttacactt gtaaattgat aaataggaac
     2701 taattcaact cctgataata tcaattcttt tattacttca tctatccctg cccctttcgc
     2761 tagatatact aaaaaactac tgcttgcttt cacattgtat agtttcttaa tatccttaga
     2821 agctcgttca agagtgaatc cttgtttaac aaagtagcct ctgttttcta ttaaagcatt
     2881 tttcacgctg ttatccctga catttcgtag gcgttcacaa acagtgttaa taagttcttt
     2941 ttctgtcatt agatatgtcc cttgccacga taagccctgt aaaacttcat gtgcttcatc
     3001 taaaatcaat tgtttttcct tgctaagttc tgatcgggaa ttttgaggaa atactctttt
     3061 agcctctttt tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa
     3121 atgaaatttt tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc
     3181 attaaaaacg tctaatcgat agacattgct tctcttatgt ttaaagtttc ctaacgcttt
     3241 ctcttccttt aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa
     3301 tttttttatc aatcctataa aagtcataag tgatagtaac ttattacatt tatctatgtc
     3361 ataagtttta ccatagtaag aactcatttt tttagctaat tgttcccccg acaaaataca
     3421 agtcacactt tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac
     3481 acgtcgcatt tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc
     3541 aagagtgtca aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa
     3601 gtctaggttc tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag
     3661 catcacaact ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat
     3721 ggaatgccca gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag
     3781 acatcttaag tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc
     3841 ataaatctca ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt
     3901 catttcttct ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc
     3961 atactctctc cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg
     4021 aagctcagta atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata
     4081 tcctccaaaa aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt
     4141 tttcaagtat tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag
     4201 aaagacaatc ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa
     4261 cgcagaatta tgaactaaat tgatagataa aaccttttct atgcttaagt atctactatc
     4321 atctttactc aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag
     4381 aataattact ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct
     4441 tggaataaat gattgtgatg aatttagtat ctctacaaac tcatctcttg taacttctct
     4501 atagttatct aaacacctat ctcttacatc agttttttct ttttttgtaa ctggcttctc
     4561 taataatttt tctttatcta cataaattgg aatattaatc attgatgttt ccatttgtgt
     4621 ttcctcctta taaaattctg ttaaagacta aaacatacag at
//
LOCUS       DAFFSV010000160         4614 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFFSV010000160 DAFFSV010000000
VERSION     DAFFSV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.161"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(15..962)
                     /locus_tag="LR110_002681"
     CDS             complement(15..962)
                     /locus_tag="LR110_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBL4823603.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1140..1943)
                     /locus_tag="LR110_002682"
     CDS             complement(1140..1943)
                     /locus_tag="LR110_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBL4823604.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2089..3003
                     /locus_tag="LR110_002683"
     CDS             2089..3003
                     /locus_tag="LR110_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL4823605.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3051..3521
                     /locus_tag="LR110_002684"
     CDS             3051..3521
                     /locus_tag="LR110_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBL4823606.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3606..4214
                     /locus_tag="LR110_002685"
     CDS             3606..4214
                     /locus_tag="LR110_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBL4823607.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4348..4548
                     /locus_tag="LR110_002686"
     CDS             4348..4548
                     /locus_tag="LR110_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL4823608.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1510 a          716 c          911 g         1477 t
ORIGIN      
        1 attgttttca gctattaata tcctgcagac aattttactt gattggtttg aagctcatgg
       61 gtggcaaagt agcttttcag attggttaaa aaaatagcca ttaatttctt ttgaaaatca
      121 ggtgtcaaac ctgcaatgtg ggaagtaatc agtacattgt ccattttcca aagaggatga
      181 tcttgtggaa gaggctcttc ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta
      241 tttaaagcct gcaccagatc ttgtgtctta acagaagcac cacggccaac attgatgaat
      301 acagattttg gatcaaagtg ttcaaacata tgctgatcaa agagcccagt tgtttcctcc
      361 gttagaggta agatattgac tacgaaatct acttcaggaa gaacttcctt tagttgattt
      421 aaagaaaaag tcttttccat tccttctgct ggatgaccgg atgtattgat tccatagcag
      481 ttcatgtcta atgctttgca tttttttgca atcatttttc caatatggcc agttccaatg
      541 attagtattt ttttcccttg gatagattgg taatggaccg atgaacctaa ccatttcatg
      601 tcactctgtg ccttttgtgc ttgaaaaaga ccacgagaat acccaaggat gattcctaag
      661 acatgctccg taatcgattc actgtggatg ccactaccat ttgaaagtaa aatatgtttc
      721 tttgaaaaag tctcaagggg tagctgatca acaccagctg agatagattg gacccatttc
      781 aaggtagaag aggctaatag tttttcttgg tagtctgagt tccagcccaa actgatctct
      841 acattttgta gatcctctgg ttgtaattct gttttaaaga tataaccagg cgctatagta
      901 tggatttctt cgataaattc atcacgaaat ggacgatctg cataaatgat tctttctgac
      961 atagttaatt tcccgccttt ctttactcta agtgtagcgc atacaagaag aactttgaag
     1021 cgccttgctt gttttttgag aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa
     1081 aaatgtcaca acctctaaaa gggtctattg aagtagaaat aacgagttgt ctttatttct
     1141 taattgatcg atatttttgc ccatcctttg acagcttgag caaaaataaa gattcctgca
     1201 aaagcaatga aggcgctagt gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt
     1261 atagtaggca atgaaattga atagatcaat gtttttaaac aatcaagaaa agtagctttt
     1321 gttagtcgaa agcgtgcata gagaaaagca gcaatgttgg taaatagcaa agtgatcagc
     1381 agattcagaa aactaggata aatagaaaat ataaatgtca ggacttttat ccaaagtggt
     1441 atagatgctt cacttaaaaa agaacggagc tgcttacctg ttaagttttt taaagattca
     1501 ttggtgtagg gaagttctaa ttggttattg ttcaacaaag cagaagtcgt gcctgtatta
     1561 ggaagagcta tgataagttt gttttttaac atcccgacac taacaaaatt ccccatcaaa
     1621 tcagtagaga tgtcctgttc tgttcgtttt ccctctggat caaatgtgaa gataatggag
     1681 tttgtttggt aaatgaatcc ttctttttct tcggtttgaa gctgcccgtt ttggattgta
     1741 aaatcaggaa tgcgagtagc aatcttttgc ccatccaatt gaatgttgtt taagacttga
     1801 aaaacttgaa aagagattgg caaagccata attatactca aaactaaaag atagacaatg
     1861 gctttccaaa atgggacatt tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg
     1921 ataattaatt gttttgttgt cataagtact cctcatctag tagtgtcatc acttgcaatt
     1981 ctagcgaagt atcaagtgaa atacaagtgc tttaaggaaa cagctgaatg attggtgaaa
     2041 tatcaaaaaa atgttattct tgtctaaaga ctatccaata taaataaaat ggaggaagga
     2101 atagtgccta cacagccaca gagatcatca aaaaaactat ttggagtagt ggttttgaat
     2161 gcggtgatca ctttatcaga atttattgga gggattatgt cagggagttt gtctcttgtt
     2221 tcagatgctt tccataacct tagtgataca ttggcaatta ttttttctta cggggcacaa
     2281 aaaatggccc gaaaagaagc aaataaaaaa cgtacgtatg gctaccagcg tttagaaata
     2341 ttgagtgctt tcatcaactc attcatattg atcatattat ccttgttttt aaccgttgaa
     2401 gcattcaagc ggttcacttc accagaaaag ataaatagtc atttgatgct aacagtagca
     2461 gttattggat tgctcgcgaa tttgttctca gtattattat tacgacaaga agcagatgaa
     2521 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
     2581 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
     2641 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
     2701 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
     2761 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
     2821 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
     2881 tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa
     2941 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
     3001 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
     3061 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
     3121 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
     3181 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
     3241 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
     3301 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
     3361 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
     3421 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
     3481 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
     3541 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
     3601 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
     3661 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
     3721 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
     3781 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
     3841 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
     3901 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
     3961 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
     4021 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
     4081 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
     4141 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
     4201 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
     4261 agagcgaaag cttgctagcg ttttctaaat tgggtataat gttcttgcaa ggttacttgc
     4321 aagaactttt ttggaggata tacatttatg gaacacggaa cggttaaatg gttcaacaat
     4381 gaaaaaggat ttgggtttat tacagtagac ggcggagatg acgtatttgt tcatttctca
     4441 gcaattcaag gagacggctt caagagccta gaagaaggtc aagaagtaga attcacgatt
     4501 gttgaaggtg cacgcggacc tcaagctgct gaagtgacta aattataata atttgttgaa
     4561 taaagacacg acttcttaga gagtcgtgtc tttttgtcta tttatgaagt tttt
//
LOCUS       DAFFSV010000161         4430 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFFSV010000161 DAFFSV010000000
VERSION     DAFFSV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.162"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            822..1556
                     /locus_tag="LR110_002687"
     CDS             822..1556
                     /locus_tag="LR110_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4823609.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1572..2741
                     /locus_tag="LR110_002688"
     CDS             1572..2741
                     /locus_tag="LR110_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL4823610.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2757..3752
                     /gene="lacD"
                     /locus_tag="LR110_002689"
     CDS             2757..3752
                     /gene="lacD"
                     /locus_tag="LR110_002689"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL4823611.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3831..>4430
                     /locus_tag="LR110_002690"
     CDS             3831..>4430
                     /locus_tag="LR110_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBL4823612.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDI"
BASE COUNT         1502 a          745 c          957 g         1226 t
ORIGIN      
        1 cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta actttaactg
       61 ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac aatcggaata
      121 gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt gccattccaa
      181 agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt gactaaacga
      241 agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat tcagataaac
      301 tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc ttaagagaga
      361 acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat ttattctcat
      421 agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga tttgaaacca
      481 aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca ctggtaagta
      541 gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta aaaaaagata
      601 ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa aagttggaaa
      661 attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat attaacaaga
      721 ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa ataccactac
      781 tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat acctttcaat
      841 caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa aacagtatgg
      901 tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc gttagtcgca
      961 cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat cgacaacatg
     1021 gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct ggcgcatata
     1081 gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata ttggccttca
     1141 agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta ggtgagcctt
     1201 tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc gagaaatcct
     1261 atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg aaacctttat
     1321 ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag gaattttatg
     1381 ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga tcaccagtgc
     1441 tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt acattaagtg
     1501 tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact aactgaattg
     1561 gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga gcagaaaata
     1621 ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac cgttatcaag
     1681 aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct ggagagcgac
     1741 caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat acactaattc
     1801 cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt ggttcgaccg
     1861 atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc ttggtctcat
     1921 ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc aatcagctgg
     1981 tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg gctacacaaa
     2041 tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat gacgctggtt
     2101 ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt tttgatcaag
     2161 caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga gaagaagtca
     2221 tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc atcatttact
     2281 taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt ctcgagttaa
     2341 ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat ggtcccaaat
     2401 cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca tatacgcaga
     2461 aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa cgcgttgttg
     2521 gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt gcagattcgt
     2581 tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg atcgcattac
     2641 tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga acagtcaatc
     2701 gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg tgaagcatgt
     2761 tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca gaaggcatta
     2821 ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg gctttaggga
     2881 tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa gaattgactc
     2941 cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca cagaagcgtg
     3001 cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa accgtacccg
     3061 gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag ttaggtgcgg
     3121 atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc aatcggcaaa
     3181 agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg ccattttatt
     3241 tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc tatgccaaag
     3301 caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag tataaagtag
     3361 atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat gcaacagggg
     3421 agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc gatgcaacag
     3481 atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg gagacattgc
     3541 gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga gcaacttgga
     3601 aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa tggctgacag
     3661 aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact gccatcccat
     3721 ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt agtgataaga
     3781 gatatgtgtg attataattt atcattattg aaactgggag gatattttat atgaataaaa
     3841 gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa ttaaagcagg
     3901 caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt agctgtcctt
     3961 acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct gatttaattt
     4021 tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta actctaaaag
     4081 agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta aatttagaac
     4141 ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt ggtagaatcg
     4201 tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt tgtttaactg
     4261 gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca ttatccaaat
     4321 caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt gatgaaccaa
     4381 ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
//
LOCUS       DAFFSV010000162         4413 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFFSV010000162 DAFFSV010000000
VERSION     DAFFSV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.163"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..180
                     /locus_tag="LR110_002691"
     CDS             <1..180
                     /locus_tag="LR110_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823613.1"
                     /translation="ISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYG
                     DLIRRVIGCGGLEFF"
     gene            180..551
                     /locus_tag="LR110_002692"
     CDS             180..551
                     /locus_tag="LR110_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823614.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            544..939
                     /locus_tag="LR110_002693"
     CDS             544..939
                     /locus_tag="LR110_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL4823615.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            941..1318
                     /locus_tag="LR110_002694"
     CDS             941..1318
                     /locus_tag="LR110_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823616.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            1319..1927
                     /locus_tag="LR110_002695"
     CDS             1319..1927
                     /locus_tag="LR110_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4823617.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            1927..2268
                     /locus_tag="LR110_002696"
     CDS             1927..2268
                     /locus_tag="LR110_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823618.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            2510..>4413
                     /locus_tag="LR110_002697"
     CDS             2510..>4413
                     /locus_tag="LR110_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBL4823619.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLIN"
BASE COUNT         1565 a          564 c          909 g         1375 t
ORIGIN      
        1 attagtgaag tgttgtataa aataataaaa aatgaatcag aaaaaacagt atcttcagtt
       61 agacgtggcg atacaacgat tagttatgct tcaacaacaa atgatgttag tgagttactt
      121 ctaggctatg gtgatttgat acgaagagtt attggttgtg gaggattgga gtttttttaa
      181 tgaatgaagc agatatttta gaaactactt atgaagatag ttgtattatt gaaagactga
      241 cggacattga agattctaat acaaatatta ctattcaaga ttacaaaaaa gtatatgata
      301 atcctatttc ttgcgctctt tcacaaggtc aaatcgatgg actagcagtc atagaagatg
      361 gagagatggt aaatgtttca actgacacat ataaattatt tgttcatcct aagattaaac
      421 taaaaaaagg agatcgaata acaataactc aaaaggccag tggcttaatt ttttctctat
      481 ttgctactaa gcctttttac tatcctagtc attgtgaagt gaatttgata ggaagtgaga
      541 aaaatgggta atctcaaata tgaatcaaat gcagaaaaga tcattgagaa ttttaaaaat
      601 atgactgtaa ttgctcaaaa agaaggaata tccttcgtta acgattcaat gaataaagtc
      661 gttagtttag ctaaaccatt aactcctgta aaatcaggta atttaaggcg aggatatcgt
      721 gtagtaaaag ctagaaaact atcaagcggt cgaattgttg gagcagttat aaacaatgaa
      781 cattatttta aatatgtaga agaaggtcat aggactaaaa atggtggctt tgtcaaaggg
      841 aaattcatgt taactcgtgc aacgaatctt gcaaatatgt cttatattcc tcgaagattt
      901 aaacaaatgg caataaaaat cgttaagaaa ggaaagtaac atgaaagatg aaatcattgc
      961 tgcaatcagt agcaaattaa aagaaatcta tcctgatggg acaatatatc ttgattcagt
     1021 tatgcagtca actaaagatt tttatttcgt tttatcagta atggaatctg gaactgaaaa
     1081 tgtaggaatt gatattcaaa atgtttcttt cttaattgat attgcattga ttgataataa
     1141 acctaataga aatttaatta atgaattagt ctcacgttgt gggacttttt ttaatacgat
     1201 tacaatcgat gagcaaatat tatttccaaa agcctattta cctgatgaag cagatggtgt
     1261 tcaacatatt cgttttacat tagaatttcc acaatatatt gaatggagtg aaagataaat
     1321 gggagaaaaa agaagtaaaa ccggaattat ttctgtcgaa aaacctactt ggtttccttt
     1381 ggaagatgag acagggaatt ttccggtata tggtactgcg ttgccaatgg gaacagcagt
     1441 aagtatcaaa ccaacagcta attatgaaac aacacaagat tatggggatt cagtggttca
     1501 agatcaattt acagcttttg gtggtgcaga agttgaactg gaagcaaatg gatatactca
     1561 taaagtttta tctacaatta ctggagggaa aattgttaaa ggtggtgcct tgcgttctgg
     1621 agaagatatt gcgcaagatg gtgcgtttgc atatagacgt aaaaaatcga atgggaaata
     1681 ccgttatacg gtcttttata aaggacagtt tgctctagat tcagatgaaa catcaacgat
     1741 tgaaggaagt aaagtaagtt ttactcatcc aacttggaag ggttcatttg tagatgtacc
     1801 aggacttgga tatatgtatt cagttgatga agatgatgaa ggcgtggata aagcaatgat
     1861 cggaaattgg tttacaaaag tagcaattcc aattgaagaa gcagaacttt caggaggtac
     1921 agaataatgt caaaatatca aacaacgatt aaattaacga aaaaagatga agaaggtaaa
     1981 tatgagcaag tacaatttaa atctgctgaa tttttaccag gaacagttgt agaagatgct
     2041 gcaggagtta tggaagaaat gcaaacagcg actgataaac aatcagttaa aaaagcttta
     2101 agtcgtgctt attcatttat tgcagatact ttatttgagg gacaatttac tggtgaagat
     2161 tattgcaaag gaattgatgc tcgtgagatt gcctcattga caggaaaatt attgaagtct
     2221 gttactgcag gttttgatga aacttataca gagacgaaaa aaaagtaagt gaggcgctca
     2281 agtcaccttc atttaagtat tcgattactt accgagaatt agatataaag acgcaattac
     2341 tcgaagcagg ttggacgtta ccagaaattg aacgtactga cttggatgag ttaatgcgtc
     2401 tttttgcttt tagagatgcg gtaaaagagc atgaagatgt tgagtactat gataatttca
     2461 ctcaatttta ggaggtgata ctttgaataa cgatgaccta attctgaaga tgatactaga
     2521 tgaatctggt tttactgccg gtatgaataa tgctgtcaaa aagttgaact cttttgatga
     2581 aacaatcgaa agaaaaagta gaaatagtgg cagttcatta ggtagtattt ggaaaatatt
     2641 tgctggaagt tttctagcta gcggagtaac tagaatcgta ggagctggct ttgatctaat
     2701 caagggttcc ataagtggag cggttgatcg agtagatacg atgaataacg ctctacgaaa
     2761 tttccaaaat atgggattta gtaattcaga aattatgaag aatatcggaa agaatgggct
     2821 tttatctcaa ggtattcaag gacttcctac tgctttgaat gatgcgataa gtcatgttca
     2881 acttcttgct tcttctacag gtgatttaac tcgttcaact caaatattta aagctttaaa
     2941 tgatggaatt cttggtttcg gtggttcaac tgatcaagtt aatgaagctg ttattcaatt
     3001 atctcaaagt ttctcaaacg gaaaagtaga cgcacaaact tggaactcaa tgatcaacgc
     3061 tcaacttgga cctactcttt ctgctattgc taaaaagatg ggaattacaa tgggagatct
     3121 gaaagaaggt ttgtctcaag gtaagatttc tgttgaagaa ttccaaaatc aattaataga
     3181 aatggatacc aaaggtggcg gaggacttaa atcattaagt caaatcgcta aagattctac
     3241 taagggaatt aaaacctcta tacaaaatgc aaaaacagct gttacacgtg gtgttggtga
     3301 ggtaatagaa ggattaaata aagcattagt agattcagat ttaggtggat ttaagggaat
     3361 tattgataaa gtgggcagtt caatggaatc gttcttaaaa gtaattgctg caaatattcc
     3421 tacagcagta tcatttttaa gtaatttatt taacgaaatt caaaaatttg gttctgcttt
     3481 gaaattcatg atgccctttc ttgttcctgc agctaccgct tttggagcgt tcatgtttca
     3541 acttaaaggt ataccagcaa taataaaaag cttcaataat tttaagaatg ccataattgg
     3601 cgttggaaat tcattaaaga ttatgggggc aatagctgct gctaattcat ttgttttaat
     3661 tattggtgca gtcgttggtg caatagctgt tttcggttac tttatggcaa ccaatgaaga
     3721 gtttagaaac aaggttatat ctatttggaa tgatgtgaaa gattccgtaa ttggtgtatt
     3781 aaaaaatata aaggactggg gaattgatac ttggaattct gctaaagaaa tggcatcaaa
     3841 tgcagttgag ggtgtcaaag atgcttggtc aggaacaaag gaatggtttt ctaacacctg
     3901 gaaaggtatt aaggatgggg ccacaggttt atttgataaa acagtagaga cttcaagaaa
     3961 tgcagtcgat agtgtaaaaa atgcatggtc taatatgaag aaatggtttt ctgatacttg
     4021 gcaaagtata aaagattcag ccagagaaaa atggaatgaa attaaaggtt ctattatgtc
     4081 ggttgctggt ccattgatta caggtataaa gaatgcattt ttacatgtta ctttttatct
     4141 agagacctta tggaataatt tagttgagat tggtaaaaac gtctttgaga ttttaaaaaa
     4201 tgttatactt gcgccggttt tgtttattac ctccatgatt tctggtggat gggaagaaac
     4261 aaaaaataat atgattgctg tttggaataa tattaaagaa agtgccataa atatttggga
     4321 atctataaag aatatatttg taagttattt tacaaatatt tattttgctg cactcaatat
     4381 ttggacaggg ttcaagctta cattgataaa tat
//
LOCUS       DAFFSV010000163         4235 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFFSV010000163 DAFFSV010000000
VERSION     DAFFSV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.164"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(74..1882)
                     /gene="pepF"
                     /locus_tag="LR110_002698"
     CDS             complement(74..1882)
                     /gene="pepF"
                     /locus_tag="LR110_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBL4823620.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2058..3056)
                     /locus_tag="LR110_002699"
     CDS             complement(2058..3056)
                     /locus_tag="LR110_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBL4823621.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3282..3935)
                     /locus_tag="LR110_002700"
     CDS             complement(3282..3935)
                     /locus_tag="LR110_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBL4823622.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         1239 a          896 c          657 g         1443 t
ORIGIN      
        1 tacgtcgact cccttacaag agagccgacg tatttttttc gtttctattc gagaaaatca
       61 aaaatagaaa gccttataaa cgatcgatca attcttctaa ttcatttaga cgttgttcaa
      121 acatcgacat tgcatcttca atatatgcag cctgtgtcat atcaactcct gcttttttca
      181 ttacttccac aggataatcg ctgttacctg cttttaggta agccaagtag ttctctagtg
      241 cctctggttc ctggtttagt attttcttcg ccagtgctga agcagcagaa aagccagtcg
      301 agtattggaa aacatagtaa ttataataaa aatgcggtat ccgtgaccat tcgaatttaa
      361 tttctgggtc ttcttcgact gctggaccat aatattttgc attcaattta ccataactat
      421 cacttaggta ttcactagtc aatggcacac ctttttcatc ttccgtatgc atgaaatgct
      481 cgaattcagc gaattgtgtc tggcggaaaa ctgttccttt aaacccatcc aagtaatggt
      541 tgagtacata agcccgtaca cgagggtctt tttctgtttc caataaatac tccgttagga
      601 tattttcatt cgttgtcgaa gcaatttctg ctaaaaagat ggagtagtcg ccgtacacat
      661 aaggctggtt cgaacgagtg aaataactat gaacactatg tcccatttcg tggacaagcg
      721 taaatagctg atccagcgta tcatgccaat tcaataaaat atatggattt gtgtcatagc
      781 ttcccgaaga ataagcaccg cttcgtttcc ctttattttc gacaacatcg atccaacgtt
      841 cagagaatgc tttttctacg atggccatgt attcttcacc cattggtttc agtgcttcta
      901 aagctttttc ttttgcttct tcgtacgtaa aggtaattgg agcttcccct aagaccggtg
      961 tataaagatc atacatgtgc agtttttcca cttctaataa acgtttccgt aattccatgt
     1021 atcgatgtaa caaaggcaaa tgcttgttta ccacgtctac caaagtatcg tatacacttt
     1081 caggaatatg attattgctc aaagacgctt ctctggcaga gctgtaatta cggactttcg
     1141 ctttaaaatt atgtcctttt atatgtgtgc ctaaagtaga agcaaatgta tttctaaatt
     1201 gttcgtaaac actgtacaat cccttaaatg ctgcttcgcg cacccttcga tccgtgcttt
     1261 ctagcaactg accatacacg ccatgagata attggactat ttcaccattt tctccttcaa
     1321 tcgttggaaa aactagatct gcattattca aaacagcaaa tgtatcactt gaagcatcaa
     1381 agatttcacc tgctccagca agaagagatt cctgctcagc agataagaca tgggctcgat
     1441 tatctactat ttgctggata taatggcgat agacttccaa ttttggttct tctttgaaat
     1501 attgccaaat ctggtcatct gacaattgca atatttctgg ttcaaaccag gaaacggctt
     1561 cactaacttt cgaaaacaga ctgcttgctc ttgcataaag cgcttggtaa tctgtatttc
     1621 cagtgtcttg atcgttttta aggtgcgcat atacataaat gacttcagtt tggcgataaa
     1681 ccctcaatac gaattcaatc gcatttaaaa attgagaagc gccttgatca agtgttcctt
     1741 tgtgtttttc agattgtttt aactcttctg ataattccaa atatttctca tcaaactctt
     1801 ggtcgcttga aaagatcttg gtcaagtccc aagttaaatt ttcaggcaat tcttctcgtt
     1861 ttggcaactg ctttacttcc atgaaatcca ctccttttct tcccttagct ctatctatca
     1921 aaatagatca tagtctaaca tccacatgtc acagcatcta acttttctac tcattcatgt
     1981 gtctgcttcc atcaatttta gcataactaa atcttgtttt acatagaaaa ccgattccta
     2041 gcctattttt atacttacta ttttttcaaa gaagacaagt gtatgcattc gttaaaaaac
     2101 tgctgataga ccatctcttc atcaatcata ggaaaaagcc attccctttt atgttctttt
     2161 atttggcaaa gccatgagtt gaaatcagga actttctggt tttccaaggc ttcttcgtac
     2221 aacattcgat aaagaaaaac ctgttcttgt aaataaaaga aaaaccgact tttttgatag
     2281 atccacgatg atagatgcaa aagatgttta tgacgcaagt agcattgttc ttgtatggat
     2341 actgtattct tttgcttgtg aataagttgg cgtgtcagcc atttcttccc atcttcactc
     2401 actttttcta tttttttcat ttggttcatt ttaatttcgt tactagtgaa tatttcttta
     2461 aggtccctac tagaaaaagt ccagcagcta ctgcaaaagg aaatatgccc tgatacagtc
     2521 tctgtaatat gatgatacaa ataaagcttt tgtttcttga gatcagcttt ccagcaatgc
     2581 actcctcttt ttctattata gtagcagcaa cttttttcta tcaatgagaa ttgcgattgt
     2641 ccaagaaaag aattaccaag tatccaccat acttggtaac tgttattcaa ataattttgg
     2701 gttctttcaa taaatctctg gtgagacagt ctagaacatt ggatttctat tgctagattc
     2761 gccaacaaaa tatccggcct ttgccgcaac ctcggcaaat aggcttccaa aaatactggt
     2821 tctactgact gtcccagcca atccatgaag cattccttta actgtaagtg ctcttttgat
     2881 tctctttcac taaagaccga acagttatct gatttttgat gggcaaaatg agcacatttt
     2941 ttctttccct ttttcaaaat cactcttttt cgacagccac gacaataata tttctttcct
     3001 ttttctgcat gtttagcaaa acagcactta ttatattcat tcattgcaag cagcattttt
     3061 ctccctctga acttatttac gtctaagaat ttatttacac gaaaaaagag ggcgtgaaaa
     3121 agctattctg catcaagtaa taagaacagc taaacaaaaa tagttcccaa tatttttcgt
     3181 ttggttgggc ttgttaccgc agatgcaagc ttttgaacac atttatacga aaaaaagact
     3241 gcgacacaat ttttgtcaca gtctcttact ataatgctga tttatacaaa ataaaatttc
     3301 gttcgctcga tagcattacg cgccatcaga cattgtccat attcattcaa tgtttctggt
     3361 gtacgatcgc tcttttcagc aaactccaaa attcgagcaa tctgattctc tacatccgct
     3421 tggttgagat tttccatcca gaagtgaacg actaggtaat agcgctcttc gaataaatac
     3481 aaatcagtcc aagcagactc tagttcgaca tctgttgcaa gttggatcac ttgttcaaaa
     3541 tcttcaaaag caaaagttgc ttgtctttcc tgttcttcta gggcgcttcc agataattct
     3601 tcctccggat cgctgccaat aatctgctga cgaagaaagt cactcacatc ttctgaatca
     3661 acagtagaat cttctgattg agaaatttct tcatccggaa gatttttact gataaacagt
     3721 tctaatccgt ctccttttgg caaaacttgg aaagtgacag cttcgcttcc tcggaactcc
     3781 tcttcgatat caacttcttc caaaatacta taaaagaaat tttcgatttc tttgtgattg
     3841 ccaagtaaat ccaaaaatga aacgccccgt tcctctaaat cttcatgacc aattagtaca
     3901 cggatcgtat tttcattaat atgttccatt tccattttgc tacacctcac tttgccacaa
     3961 tgtttaatat agattcattg tagcgaaatc agcttaaatg taaagaaaaa aaatctcgct
     4021 gttaagcgaa tttaataaaa ccagcggatt tttcaacctt tcaagctatc ttatttaact
     4081 ataacatttt tggcagacga atactatata gccgactttt cagctaattt tccctctcta
     4141 tttttcgtgc aatcatcatc ttctactacc tttttataca tctaatcaag aatacttttt
     4201 gtaaaaacaa aaaaacttgg aaaaactatc tttgt
//
LOCUS       DAFFSV010000164         4234 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFFSV010000164 DAFFSV010000000
VERSION     DAFFSV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.165"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..706
                     /locus_tag="LR110_002701"
     CDS             <1..706
                     /locus_tag="LR110_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBL4823623.1"
                     /translation="LKSSEDLSQHDLLFSKFAADYIQELIKKANVVALSWGDTVYKVI
                     DQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQKAAERYESSYLTLSIPLYVINETA
                     RELMALEPSLANTLATASHADMLISGIGTLSSIDSVEPWNKHRKILFPSMTDTVGFLY
                     GRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGICKSKFKAESCLGALNGNFFTDLF
                     LDEKTAWKILSLIKKE"
     gene            complement(1003..1551)
                     /locus_tag="LR110_002702"
     CDS             complement(1003..1551)
                     /locus_tag="LR110_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823624.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1552..2409)
                     /locus_tag="LR110_002703"
     CDS             complement(1552..2409)
                     /locus_tag="LR110_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL4823625.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2695..2982)
                     /locus_tag="LR110_002704"
     CDS             complement(2695..2982)
                     /locus_tag="LR110_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL4823626.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(2972..3301)
                     /locus_tag="LR110_002705"
     CDS             complement(2972..3301)
                     /locus_tag="LR110_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL4823627.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(3378..3650)
                     /locus_tag="LR110_002706"
     CDS             complement(3378..3650)
                     /locus_tag="LR110_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL4823628.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3650..3913)
                     /locus_tag="LR110_002707"
     CDS             complement(3650..3913)
                     /locus_tag="LR110_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL4823629.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(4019..>4234)
                     /locus_tag="LR110_002708"
     CDS             complement(4019..>4234)
                     /locus_tag="LR110_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823630.1"
                     /translation="IKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMIT
                     DIYVEDEVIIKYMKPVIKLVNKNKGNG"
BASE COUNT         1243 a          812 c          586 g         1593 t
ORIGIN      
        1 attaaaaagt agtgaagact tatcacaaca cgatttatta ttttcaaaat ttgctgccga
       61 ttacattcaa gaattaatta agaaagccaa tgtggttgca ctatcttggg gagataccgt
      121 ttacaaagtg attgatcagt ttaaaatggg tgctcgtgaa gaattaattt ttactcaatt
      181 cattggcgaa atcggaaaat ataattcctt agctggctcc acacgattag ttcaaaaagc
      241 tgctgaacgt tatgagtctt cttatttgac tctcagtatt cctttatatg tcatcaatga
      301 aactgcacgt gaactaatgg ctctagaacc ttctttagct aataccttag caaccgcttc
      361 gcatgcagat atgttaattt ctggaattgg caccttatcc tctattgatt ctgtcgaacc
      421 ctggaacaag catcgtaaaa tcctatttcc aagcatgacg gatactgttg gatttcttta
      481 cggaagaccc ttcaatatca atggtgaatt catcaaagca tctgaggata agacttttgg
      541 tctttcttta aaagaaattt tcgatattcc aaaacgaatt ggtatttgta aaagtaagtt
      601 caaagcggag agttgtttag gcgccttgaa tggtaatttt tttacagatt tatttttgga
      661 tgaaaaaact gcttggaaaa tactttcttt gataaaaaaa gagtgagtca tgtaaaatcc
      721 tccaaaaaat ttagattttt ggtactactt ttttggaggg cagtacaatg attcactctt
      781 tttattggat atcaaaatcc ctttttttga gaagcttgtc ttgatatttt ttgaactttt
      841 ccaagccata ctccattact tttcaagatt taatttcttt gtattcactg aataatttcc
      901 acgttttttc gttaatgtta cttcttatta attttattgt acttctcatg caataggatc
      961 atatatcctt ctaaatcttt tttatgctat aaaaaggggg ttctatctaa ctttattatt
     1021 cattccccac cctttgcgca gtgagtcttc ggtatcttgt tttatctgac tggtagttct
     1081 tattatttct ccacggtcat ctaattctgg aacaattgga tatttttctc tgaacaagtc
     1141 tagttctttc accacgtcat taaacaaatc ttgcttatct tcttgcgtaa gattctgttt
     1201 aatatactca tctaaagctt ttctaagcaa ttcatctctt gtcatattag tctcatactt
     1261 ttctactaaa actctggaag tcgcatttaa caaagtcgac agttttaaag gatgcttggt
     1321 ggaataaacc ttatcaaact ttctgattgg tgcgccaact ttttttgttc ttcctctact
     1381 ctgtctttca acattggagg aagtttttat ttcttgaact tggtgcactt ctaattgctt
     1441 attttcagat ggtgtaattg cttgctgctt tttctttgct tctgcttctt tttttatttt
     1501 ggcgagtcct ttgttgttcc caaaaatatc aagtcctttg atgtcagcca attactccac
     1561 ctccgcatac caattctcgt gttcttccaa ttctttcaaa atactgataa ataattcgtg
     1621 gcttttgcga tcccattgtt ttaagagacc tttctttgtc ttctccatcg taatacctga
     1681 tccgtcgtat cgttttaagc gcgcattttt gagtacaaca tttttaatca aatgttcccc
     1741 ataaatctct tgggcttgtt tatacatttc ctgatctact gcgtcaccgc tttccaacat
     1801 gaaaggaata acaccagcaa catataaatt tgtatcaaat tctttcacaa aaaagtccat
     1861 atactcaaga tattgcatcg catttctcat cgattttact tgggtctgta agacgacgat
     1921 tgcccagtcc gcaatgtaca tcgcggattt actatataca gaaatagaag gtgggacatc
     1981 taaatagatg acgtcaaact catcaaataa tggttctagc ttttctttca tatatttgaa
     2041 ttgggataat gaatcattat attggtcttt gatccaatca tcatacattt caaaggacat
     2101 atctgctgga agtatataga ggttattgat tactttatag acagcctctt taggtggaag
     2161 attgtttatc aacacgtcag tcacagtatt ttcgtaaaca atcggttcac cttctccgcc
     2221 tccttcttcg ttagtgaagt tcatatgcgt taagttcatc acatcagaag tatttccttg
     2281 catatccaag tcaattatca aggttctttt tccttcatta actgctgatt caaaccctaa
     2341 catttgcgcg cacgtacttt tacctacgcc acctttaaaa ttaccaatta catatttctt
     2401 cgctaccacg ttcctacctc ttttctttta gcaatattca tttgatacta ttataacata
     2461 tttattttta gttactacca aaaaaaagat atatatgtac taccttataa ttaataagta
     2521 aaaggtagta cataaataag gaaaagttga aatatattga tttatagctt tcctcaaagt
     2581 aatgacgtta tttaatttat cgagaagcag agaatactcg cgactttagt catgtagacg
     2641 aatcagattt tcgataaaaa agaagccgtc cttcttaaga acgacaacaa atcactagat
     2701 taattttttc aactgttcat aaaagttttc atgcgttcca cataacaata ctggaataac
     2761 cttgttttct ataatctcgt aagctactct atatgttgtg cccgcatatt tgaacccact
     2821 agaccaaata cctgataaat ccccagtttt ttgttcaccc ttatatggat caatcgcaat
     2881 ttcatcataa attaaattca aaaatttttc tttcaatggc ttctctttta gcttttttaa
     2941 atacttaatg acatgttttg aaggaacgat ttcataagcc aatttctgtt gccaactcct
     3001 ctttagtcat tactttagaa ttagtaagtg tatcttcagc aatatctcta aaggaagaat
     3061 gcattttgtt ttttctattc ttaaattcta caagaatctc atctttatcg tagccttctg
     3121 caattatatc agacaaaata tcttcgctaa aatcataaaa ttctttttgt ggttcaacaa
     3181 attcataaag aataccattc tctaccaact ttgcttcaac ttcgactcca ttaggaacat
     3241 taaaatcttt aggtactgtc aacatgattg aattgccttg ttttcttgtt tttgtaatca
     3301 tttaaatccc tcatttctaa cagtatgtaa ttacttagta aatacattat accacgagat
     3361 aatgtattta caagtaatta ctgtgactcg taatgcgtcc ttgctgaata aatttcaacc
     3421 acgtgttcat cttcatctac cttataaaca acccggtgtt gtctattcaa tcttctagaa
     3481 tacctacctt catatgtagg ttttaacttc tcaaatgatt gggtagggag atagggatct
     3541 tcctttaaag tctgaataac cttctcaaat tgaggtttca aattagactg tttgattttc
     3601 tttaaatcag ctttagccga gtttttaatc atcacagaat agttattcat tataagttat
     3661 cccaatcaat ttcatcaaca ttagtaaatc cactatcatc tttttctcgc ttacgaacaa
     3721 cacccaaagt gcctatttgc tcaagtaata gcgtttcttt gattgctttc aaatcatcca
     3781 aaccaataag caccgcatta ttttcactgc gctcactaat gatttcaatt tccgtatgat
     3841 tctcatttac atctttcaac aacttataaa aatttttccg tgctgagcta ggatttaaga
     3901 ctgtttttgc catatctact ccccctctaa tttatatctc cattctaacg tacttaatta
     3961 agtacgtaaa ttcgattgtt ttgtttaaat tggagtacca aaacagcatt gtaaagtgtt
     4021 atccgtttcc tttattttta ttaacaagtt ttatcacagg cttcatatat ttgattataa
     4081 cttcatcttc tacgtagata tctgttatca ttacgacatc tgtactattt cgattgagta
     4141 ctttcacttg atctccagca ttgattttcc ctctaaatct tttaggaatg aaccaactat
     4201 acatatgatt agcatcacca aaatgtgctt ttat
//
LOCUS       DAFFSV010000165         4219 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFFSV010000165 DAFFSV010000000
VERSION     DAFFSV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.166"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(81..1160)
                     /gene="uxuA"
                     /locus_tag="LR110_002709"
     CDS             complement(81..1160)
                     /gene="uxuA"
                     /locus_tag="LR110_002709"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL4823631.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(1163..2794)
                     /locus_tag="LR110_002710"
     CDS             complement(1163..2794)
                     /locus_tag="LR110_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBL4823632.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            3098..3769
                     /locus_tag="LR110_002711"
     CDS             3098..3769
                     /locus_tag="LR110_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4823633.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         1368 a          805 c          574 g         1472 t
ORIGIN      
        1 aaaacatatt taaacactcc tttccttccg ttagttttta gaagagaaaa tttttcctct
       61 aaataaactc ccttatataa ttatttactc gccccctttt caagcgcttc ccaaatacca
      121 tttaaataaa ctgcacctag agctcggtca tataatccat aaccaggtct accggtttct
      181 ccccaaatca tccgaccatg atctggacga atatagccat taaatccatt atcgtacatt
      241 gtttttaata tcttatacat atctagagaa ccatattctg atttatgcgg cgcttcgtaa
      301 aaatcgagtc cattgtcact taaatatttg atattacgaa catgtgcaaa aggaatccga
      361 tcccgttgac aaaattcaga tagaatttca tagacatcat tatctggtcg ttctgcaata
      421 cttccagtac ataatgtaat accattagaa ggagaatcca ctacattaca caaccaatct
      481 aaatcgtcac gatttttaac aatacgaggt aatccgaaaa tggaatatgg tggatcatct
      541 gggtgaactg ccattttaat cccaacttct tcacatgtcg ggataattgc tttgagaaaa
      601 tatgcaaagt tctctcgtaa tttctcttca tcaacttctt tataggcttc taataattgc
      661 ttaatttgtg ataaccgatg aggttcccaa cccggcaatt caaaattatt agtagaattt
      721 tctacttctc taataatttc ctccggatca gtagggatat tttttgaaat ataggctaac
      781 gtagaagacc catcatctaa acgataatcc aaatccgatt ttacccaatc aaatactggc
      841 ataaaattat aacagataac ctcaatccca aattctgcca aattgcgaat cgtctgttta
      901 taattctcaa tgtattgatc tcgtgttggt aaaccaattt tgatatcatc atgaacatta
      961 acactctcaa taactttcaa ggtaagtcca ttggcttcga cttcatcttt taaagattta
     1021 attcgttctc tcggccaaac ctctccggct ggtatatcca ttaatgttcc aacaattcct
     1081 ttcattgctg ggatctgacg aatttcttgt agtgagattt tttcttcatt tgccccatac
     1141 catctaaaag tcatttccat agttactttc ctccaacttc tgttatatat ttattgattg
     1201 ttttacgaac tgcttgagga cctactagca tttcgcaaaa gattttttca gttttctttc
     1261 ctaccccaac ttgatataaa tctaaaccaa agatttcttt attttctaaa atttcctgta
     1321 ccgcacaatg aatctttgaa ggatcttgtt cacctaattt caatactgat aatttatcct
     1381 gcaactctac caataacgga tcaggacttg gcacaaaatt ttccccttga tcatcgactg
     1441 ccagtaaata acgtaaccaa gcagcaatag cgataggaat aaatgttaga ttttctgcat
     1501 ttccgtcttt ttcgacatac tttttaattg tttcaccata gcgaatcgct actttttgag
     1561 aagtatctgt tgcaattcgt tgtggtgtat ctggaatata gggattaggc aaacgtttat
     1621 taattacttc atcaataaat tgttttggat caataatgcc tggattttca actactggta
     1681 aaccttctaa ataacctatt cctttgatta aaccaaccaa atcatcatcc tgcatctctg
     1741 ctgaaattga ttgatatcct agcagacaac ctgttactgc caaagcagta tgaagtggat
     1801 tcaagcttgt cgtaaccttc atgatgtctg ctttatcaac cgtctctcta tctgtcataa
     1861 tcacaccagc cttttccaat ttaggccggc catttggaaa actatcttca ataactaaat
     1921 aatgaactac ctcagtatta gtgaaagcag caatattggt atgttttttt gtgtgaatga
     1981 tatctaaatc ctcgagcccc atcatcaata gatcttcctt tacattctct gaaggatttg
     2041 gcgtaattcg atcaatcata ctccaaggaa aagtcacttt ttctttatcc tttaaataag
     2101 caataaattt ttcagaaaca tatccttctt tttgccaagc ttctgcaatc gtaaaaatac
     2161 tatcttgaaa acgttgacca ttctgtgaga aattatcagt agaaaccatc gcaatcggca
     2221 actctccagc attaaatctg ctatataaca aggatgttac aatactcatc gtatgttgag
     2281 gattgctagg tccagatttg atatcagctt tgactaccga aaagaattgc cctttagaat
     2341 cttttaaact gtaacctttt tcagtaatag taaacgttgt aaactgtaat ttaggatctt
     2401 caaaatattt aaccactctt tgatagtccg ctatattctt cggattgcaa tataaactat
     2461 cagccgttac tgccaaaagt ctttttttga gttcaccatc tccactcata acaatttcca
     2521 aaatgtcatt gttatagtca tgataagctt tttccactac ttcttcatca aatgtttcac
     2581 acacaactac tcctgtttct aattcatttt gatctgctaa gcattgagca atttctgcat
     2641 gaaatcctcg ataaagattt cctccaccaa aatgaagcca tactggatgt tgctggcctt
     2701 cttgcttaac ttctttgaca tcaaaacacg gcagtagaat ctgcatttca ttgaattctt
     2761 cgatagaatt ttgccaattt ttaatcgaaa ccataattaa cctcctcaaa ctgatatgtt
     2821 agttttgata cattttatat tttaaaaccc attgtaaccg ttgtcaatat aaaaagggaa
     2881 taaacttgct tttttctacc atatcagaat gattgtattt tttattcaga aaaataaaat
     2941 actccttctt tttggactga ttttatcgaa acttattttt tctttttttc atgtattctt
     3001 ttatttttat aactagtgta ttacttcaaa atttgatatt atatggcatg aacagaatta
     3061 aaatatcagt taaaataaaa gttaaggagc attgtgtatg acaattaaaa ctagtctcca
     3121 aaatcaagct taccaatcta ttcgccgaca aattatctat gctgacttag aacctggaaa
     3181 aaaagtatca gaaaaaaaat tagaagaaac actttctatt ggtcgtacac caatacgtga
     3241 agcactgatt caactccgac aacaaggtct tgttgataca attcctcaaa gcggaaatta
     3301 tatctcatta attgatttag attcggccaa aaatgctcga tttgtcagag aacatttgga
     3361 acgacaaatt atgttagagt gttgtgccaa aatgaataac caaaaaaaac ggattttaga
     3421 aactataatt tcagaacaag aaaaagctgt tagtcaaaaa aatgctcgta gtttttttca
     3481 taatgataat ttattccata aagcttgttt tgaaattgca gagcgagaag aagtatggca
     3541 atggttagaa aataacaata cacacttaga acgatttcga tggctacgag taaaaacgga
     3601 aggattaaag tgggaaaaaa ttatggaaca acattatcaa ctatttgagg cattaacaaa
     3661 acaagatcca gaagaagcta actttctaac atcgttgcat cttcatttaa tgcttaatga
     3721 gcaaaatatt gttttagcta ctcatccaaa atatttcaaa aatatataac ttttaactct
     3781 ttttaaatta aatggttgtt ctgatcagaa aaatatcgct tgttttctta ttatgtaata
     3841 tatttcataa actattctgc ataacaacta ttgtttgtac tttttacatt ggataactaa
     3901 ttatgactgc tgataacttt tgatattaaa ctagtatctc caatacaaat aaaaaataga
     3961 tatcttgttc atcaaaatta gttcgtggat acttaccttc atttacatca ttcagcggtt
     4021 tcccattttc ttttattcta ttatttcatt ttctaataga cttggcttca tttcgcaata
     4081 ctttttttca ataggcatca aattggtgtt gtttagatat ttagttgtag ttttgtgaca
     4141 tgataagtat gctccttttt ctcttttcca ctaatacaac gatcaaaggg tgcttttttc
     4201 aaaaaactag aaaaatcaa
//
LOCUS       DAFFSV010000166         4217 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFFSV010000166 DAFFSV010000000
VERSION     DAFFSV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.167"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            74..502
                     /locus_tag="LR110_002712"
     CDS             74..502
                     /locus_tag="LR110_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL4823634.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            489..731
                     /locus_tag="LR110_002713"
     CDS             489..731
                     /locus_tag="LR110_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823635.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1031..1246)
                     /locus_tag="LR110_002714"
     CDS             complement(1031..1246)
                     /locus_tag="LR110_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823636.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1353..1667
                     /locus_tag="LR110_002715"
     CDS             1353..1667
                     /locus_tag="LR110_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823637.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1771..2949
                     /locus_tag="LR110_002716"
     CDS             1771..2949
                     /locus_tag="LR110_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL4823638.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3352..3645
                     /locus_tag="LR110_002717"
     CDS             3352..3645
                     /locus_tag="LR110_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBL4823639.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1446 a          628 c          820 g         1323 t
ORIGIN      
        1 cctagattgt agtaataatg gaaagggata gaagtaaaac gagttgaatg aattttttga
       61 gggggagaat attatgggcg actcagagga aacccacatt caacatacgt ttgacagttt
      121 ttgtaagaaa gtagtctgca atgaggcgtt aaatattcaa aagaagtacg ctagatttcg
      181 ccaacggcaa atatcaatgg aaatattgga acaaaaaggg ttaggtacag agttctatta
      241 tttggaacca aatttgaata gtagtggtac ttttattgtc cttggtatga aaatctttat
      301 ttccaatgag gagttggcaa atgcgattgc agcattacct agtcaaagac aagaaatcat
      361 ttttatgtcc ttcttcatcg gtcttaatga tcgtgaaatt ggcgatatta ttgggaaaag
      421 tctcggcagt gtttggtacc aacgacaaat ggctttggaa gagctaggaa agtatttggg
      481 tgtcaaatat gaagcaaaat aaacgatata taccattcat accactaacg acaatcaaag
      541 ccgcagcttc tggtgattca attgccatgt ccatcgttct gtcaaaatat caaaattata
      601 tttcacggtt agccatgaaa ccagtttatg aagaagaaaa aggaatggtt atgtatgtag
      661 atgaatatat gcgtagacaa ctggaaacaa aattgattga gaagatactt gaatttgata
      721 caactcgtta aagtattttt tccctttcct tttcattcta tggaagtttt ttatcgggtc
      781 aaaataaagg atttccatag gctgaaaata gcctagcgct ctttgacaaa tgaatagcaa
      841 atctatcagt acatgtagat tttgattctc tagttacgcc atgacaaggt tagcttttaa
      901 gcgacaaatt ttaatatgag aatcgcgact acgataatga tacttccgta accaaatcgg
      961 cccgccatta ctggggggag gtgagattcc tatgtgcttt ggcaaaacag ctgatagatc
     1021 agaaaaacta ctatttccat atttcgattt tggttggcat atttttcatg ccaaattctg
     1081 tgattttaat agcatcggca agatttaatt tttcttcagc tctctctcca cttttaagtt
     1141 tttttactgt actcaagttg attcctgttc cgaatgctat tgcattagcc gtagattttt
     1201 caagaatttc tttaattatt tttatgtctg caattccttg tttcatatta aaaattcctc
     1261 ttttctagtc cgttttgatg gagtactgga gtgttttttt acaattttaa ggggtaccgt
     1321 ggtggtcgtt ttgattttga gagagaaggg tgatggagtt ggaagttaaa acaaatgtta
     1381 attctctgga aatagagttc tattcaattt tacaaaaaaa actggaagat tacacggaaa
     1441 aattgcttgc agtcagtcat ttacctctgg tattaacaaa tactgattta aagcgactat
     1501 ttcagataag tgatagtaca ctgaaccgtt tagtaaaact tggtgagttt cctccctgtt
     1561 ggtacgggat tcgaggacac tattttcgtg atgatgtttt agaatggatg agaaagagtg
     1621 attcgaatga ctatcgagaa aaattgcgac tacttcgatc tttataagtt acattttctt
     1681 tctaagatgc ggtacaatga tgacatagat agaaagattt ccaaagggtt tctgcttgtt
     1741 gggttccttc aaaataggag gaatcaataa atggaaaaaa cagatcgatt caagaagcaa
     1801 atcagttcta ctggtaggat cacatggtac tttcaagcat atcgaacaac aagacgtggc
     1861 tttaattcaa aacgtgaggc gcaactcgct tatctggaaa tggagaaaca acaaaggctt
     1921 aagaaaaaaa ttgttgcaag caatgacaag tttagtgttg taggtgagca atggttccaa
     1981 tattaccgtt cgttaaatga acaaaaggaa ataacctatg ataagcgaga ggaacaatta
     2041 aaaattattg ttcgatggat tggtgataaa aagttgtctg atttgacacc agagtgcctt
     2101 caagaattgc tttttacctt aaaggaatgt ggcgttaatg gaacaaatgt tggctatgcc
     2161 aaaaatagtt tacaatcact tattcaagtt ctaaatatga tttttaaata ttgtcaaaga
     2221 aagaatttga taagtgaaaa tccaatgaag tctgtgagaa tgccaaaata tcagttaacg
     2281 gtaactgatt taaaagaagc cataaataat attgaggaca agtttcttac tgtcgatgaa
     2341 ttgagaactt ttttgaatta tggcattgtt catgaggaat taccgatgtc tgtacttttc
     2401 catgttctat tttacactgg ctgccgagtc agcgaggcgc ttgctctaca gcctggagat
     2461 atagatttca aaagaaatga aattctgttc tataagcaaa cggcagttaa aggtaagagt
     2521 aaggattttc gaattgaaac tactaagaca gttagttctg ctagacgagt tctagtaact
     2581 cctctagtta tggaaaagct acaacagttg attaaggctt tgggtgagat gaggcaacat
     2641 tacaggttta ttatagaaga aacatatttg tttgtgtatc ttgattctag taaacgtggt
     2701 gttccttatc gtcgagagta tgtaaatgat cacgtcaaac ggtgcgtgaa tcgctcagga
     2761 attaataagc catttcatac tcatttagct cgtcatacaa tggcaagctt agtagcggaa
     2821 cactgtagct gggaagtctt gaaagatcgc ttaggccata ctgaccaaac aacttcgaaa
     2881 atttatcgcc acttaacgtc aaatgaaaag gtaaaacctc tgattgcttt tagttcttta
     2941 gagagttaaa aaaacggtga aagcactggt attacaggat tcttgaaaaa cgatttttta
     3001 acaaattagt caatatgtag tcagaggtaa taaaaatcct tttatatcaa tactttaacg
     3061 acttttcaca agtatcaaag aaatccgtcg ttaatcattg ttatcagact gagaaagaac
     3121 ccttggtatt caagggttct tttttcttag catgtaattt gaacaccctg atccagtttt
     3181 ttctttaatt attggtaggg gagaataatg gattagaata aatctaccta taagtagtag
     3241 taaaaaaaag agcactatga taatatcttt cttaagagta accgctttaa taaaaaatcc
     3301 agaatattct aacataaatg gagtattctt agaatcaaaa ggagaaagaa gatggtttat
     3361 acttatacgg agcaagaaaa gatgaaagtt ttagacacat tcatgaatga tggaaaagta
     3421 aaacaagtac caagttcaac aaaaaagaaa tacatcctat tgaaagaagt cttgaaacgc
     3481 tttgaccatg gagtgactta tacagaaaca gaggttaatt cgatactgct gaacgtattt
     3541 tctagtggcg attatgtcga acaaagacgt tatctaatca catttggctt tttcaaaaga
     3601 agttcagacg gtcgggcata tcagatgatg ggaattgaaa attagaagtt ttaatttata
     3661 ctcattgaaa aaagttcaag tatgcgatat tggttctaca ccgcatactt gaactttttt
     3721 tgtcatctgt atctataata caaagccaag aacgttattc aaaatatgga tagctatgat
     3781 aaaagctaat gagcgattcg cagttaaata agcaatcgtc agtaaaatcg ttccaggagc
     3841 gtacatgaaa aaatcaattg gatgcgttac atgaataaag ataattctct tttatttgta
     3901 cgaataaata ccaaaacgta cctcctaaaa ttagattttg gacagatgcc ctttaaaaag
     3961 ataacaagat ttcgttcttg aaacaatata tatgctttta ttccaatgag gttgtgaaaa
     4021 cagtattttg tcctgagcgt tagaaaattg aaaatgggga atagcaactc gtactttacc
     4081 attttttaag tgaatacaag atcagctagt tgaacaccgt ttattcgtga tgagggacac
     4141 aacactgcag tcgacacatt tctcttttat gttcaaagat aacaaatatt taaaacaaag
     4201 aaaaatagta gataatc
//
LOCUS       DAFFSV010000167         4159 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFFSV010000167 DAFFSV010000000
VERSION     DAFFSV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.168"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..911
                     /locus_tag="LR110_002718"
     CDS             <1..911
                     /locus_tag="LR110_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HBL4823640.1"
                     /translation="KTVGKVPVDLGWKKLFGKESYRKEKEEEPLLPKVTKGEAVTVDL
                     QVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLK
                     QKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQKGTQATFL
                     ANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYH
                     KAAACTSEACDFRLWTTIAKKKLTATQLKEIIQNGRTSQPVKGLKGQKGSFEATIVLK
                     EDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            927..1076
                     /locus_tag="LR110_002719"
     CDS             927..1076
                     /locus_tag="LR110_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823641.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            1157..1753
                     /locus_tag="LR110_002720"
     CDS             1157..1753
                     /locus_tag="LR110_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL4823642.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            1766..2665
                     /locus_tag="LR110_002721"
     CDS             1766..2665
                     /locus_tag="LR110_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL4823643.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            2668..3153
                     /gene="ssb"
                     /locus_tag="LR110_002722"
     CDS             2668..3153
                     /gene="ssb"
                     /locus_tag="LR110_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL4823644.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            3519..3779
                     /locus_tag="LR110_002723"
     CDS             3519..3779
                     /locus_tag="LR110_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823645.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         1504 a          733 c          866 g         1056 t
ORIGIN      
        1 taaagacagt cggaaaagta ccagtagatc taggttggaa aaagctgttt ggcaaagaga
       61 gctacagaaa agaaaaggaa gaagaaccgc tacttcctaa agtgacaaaa ggagaagcgg
      121 tgacggtaga tctacaagtc cttgaaaaag aaacgaagcc accacaacct tataccgaag
      181 gtacgttgat cacagcgatg aaaacagctg gaaaaaccgt ggatagcgaa gaagcacaat
      241 ccatattgaa agaagtagaa ggaatcggta cagaagcaac acgagcaaat attattgaaa
      301 ctttgaaaca aaaggaatat atcaaggtag aaaaaaataa gcttgtggtg acaaataaag
      361 ggattttact ttgccaagcc gtggaaaaag agccgctgct aacaagtgcc gaaatgaccg
      421 ccaaatggga aagttactta ttaaagattg gtgagcagaa aggaacacaa gctactttct
      481 tagctaatat ccaaaaattt gtctctcact tgttggaagt cgtaccagga caaatccaat
      541 ccactgattt tggatcgacg ttacaagagg tgaaagcagc cagtgaaaag caagaagccg
      601 cgcgtcattt agggatttgt ccaaaatgcc aggaacaaga agttttactc tatcataagg
      661 cggccgcatg tacgtctgaa gcttgtgatt ttagattatg gacaacgatt gccaaaaaga
      721 aactcacagc gacacagctg aaggaaatca ttcaaaatgg ccgaacaagc cagccagtga
      781 aaggattaaa aggtcaaaag ggttcgtttg aagcaacgat tgttctaaaa gaagacttca
      841 caacgagctt tgaattttct gaaaagaaaa aaacgaacta taagaaaaga acacgccgca
      901 ctacaaaata atggaggggt tatcttatgc gaaatacaac acacaaacaa caagaaatcg
      961 aaaatcaaaa agcaaaaatt cgttcagaac aagagaaatt acgtgccagt aaaagaagac
     1021 ttcagcaatt aaagaaccag caacggattc aaaaagaaat tgaattagat cgataaaatc
     1081 ttcctggcta ttgtatacgc ataaacttat gttatactat atttataagt ttatacgtat
     1141 acaaaggagg gagaaaatga caaaagcaat ggtttacaag ctgctagaag aatttaacgg
     1201 aactttggct ttgaaggatg ccaaaaaagc gggcatttct cctgtaacga tcaaacgtat
     1261 ggtagatcgt ggagagctgg atcgtgagta tccaggtttt ttcaccttac ctggtcaatt
     1321 tcctgatgaa ttgtttatgg cacaaaagaa atatgagcgc ggcattattt cgcatatcac
     1381 cgcgttggat ttatatgatt taacggatat gatccctaga cagatcgatc taaccgtacc
     1441 ttacggttat cacgtttctg aaaaggggtt gaaagaattc gcagttgagt tacactattc
     1501 aaaagcggaa tggtatgaat taggtaagat cgaaataaag agtcgttatg ggaatcctgt
     1561 tatagcttat gatccagaaa gaaccttgtg tgacatttgg aatccctggt acaacgttga
     1621 ggatgaaatc aaagtaaaag caatcaaaaa ctacatggag tcggacagaa aaaatcttag
     1681 aaaattgaat gagtatcgac gaattttgcc tacagacaaa acgatacgct cttatattat
     1741 ggctttaaat taaaggagaa gacacatgac acctacgcaa ttaaaagcaa aagtgaagaa
     1801 tatttccaga gaaaaagaag ttgatccgca gcttgtttta agacatttta tgatggaaaa
     1861 attcttggaa aaaatctcag agtctcctta tcgtgaaaat tttgtgttaa aaggcggctt
     1921 cttaattggc tcaaagtatg gcattgaaaa tcgaacaaca aaagacattg atacgacttt
     1981 acgggaaatg aaagtaacca aagaaacatt gacaacggtt ctaaacgata ttttttcaac
     2041 gccgacgaaa gaaggcattc aatttgagat tcaaggaatg aaagaaacaa gggaagctga
     2101 ttattatcca gggtttagct tacgtgtact ggcacatttg gaaaacatgc ggccagattt
     2161 taaggtagac gtgacgacag gtgactcgat ctatcctgca acgatcactc acagtcataa
     2221 attgatgttt gaaaatcgaa cgattccttt ggaatcttat cctacagaac aaattattgc
     2281 cgaaaaatta agtgcaacct ttgatttttt aacagataat tcgagaggaa aagattttta
     2341 tgatctctat actattccta aaatggaaaa gatcgatgag aagaccttga aagatagtgt
     2401 gagaaataca tttacacgtc gaggaaaaac tgatccgtta aagatttatt acgaaaagga
     2461 tatggatgta ctgaaaaata gtcatgagct aaaagatacc tggaagaaat atcaaaaaag
     2521 caatccttat gctgcgtcga tcacgtacga agagacaatg gattcgatta gtaatttgat
     2581 ggaaaaagtg attcacgtcg aagaaaaaga gcgaaaaata gcttatcaaa gacaccaaca
     2641 gatgaacaga gaaatggaga gataaaaatg atcaataatg taactttagt aggacggtta
     2701 accaaagatc cagatttacg ttataccgca agtggcacag cagtggctac ttttacccta
     2761 gccgttaatc gtaatttcac gaatcaaaac ggcaatcgag aagctgattt tatcaattgc
     2821 gtgatttggc gcaaaccagc agaaacgatg gcgacattag ctaaaaaagg catcttgatc
     2881 ggcgttgtcg gacggattca aacacggact tatgacaacc agcaaggaca acgtgtgtat
     2941 gtgacagaag ttgtagctga taacttccaa ctattagaaa gcaaagcagc gacagagagc
     3001 cgtgcgcacg ctgatcaatc gtccacgagt ccaagcacca ccacttttga acaacgggat
     3061 acagcgacgc ctaacaacaa tggattgaac gcttcacaga atccatttgg tggacaatca
     3121 atcgatatta gcgatgatga tctaccgttt taagtagcat tttctattgg ttgcatttgc
     3181 tacggtttat gttataattt agtcaacaaa aagaattttc aatctataac caaaaaccac
     3241 tagctggcgg gctagtggtt ttcttttacg tttctattat aagaattaac gagtaggctt
     3301 actgtctttc ttggcaaatt tacaatcaag ccatttgcag atatagtgcg cggctacttc
     3361 tgccatgatt gcaaaaacaa aatcaaaaag aattttcaaa cctataacct cctccccttg
     3421 ccagattagg ccggggtagc agacagcaag actattatag cataattgaa atatccaaat
     3481 gattggattt ttgctataat tttagcgagg tgattgtgat gaacaaatat gttggtttgc
     3541 ttgataaaat ccgagtaata aaaacgcaac cgttattggt tcgctttact ttacaaacga
     3601 ttcatgaatc gattaattgt gtagttgcag atattgaaat tatcgataaa ttgctaatta
     3661 tggatgatgg aaaatacaat attgccgtca caggtcactt caataaacga aatcagctgg
     3721 ttattgctag catgtatgtt agaaatcccg atcattttac acgatcgatg ggcatataaa
     3781 taaaagaaga gttgtagtcc taaatgagga ttgcagctct ttttgtttta catagaaacg
     3841 ggaacaggaa aaggcctgtt taggaaggca caagcgcctt ctcaattaaa aaacctttct
     3901 ctcactccaa ccgccaccgc cgttcgagaa gaaaggaagt gaacgctgca cgttcactaa
     3961 gatatggtgt cttgcaaagc aagacaaaat aggaaaagct tcctctcacc aaaaggttcg
     4021 ttcctgttcg ctatctcttt attgatttgc cagaccggca acaagttgcc ttttatcacc
     4081 aaaaagcgac aaggacaagg gtaaatggca cgctgcgcgc ccttgtcctt gtcgcttttc
     4141 gttgattcat tctggccaa
//
LOCUS       DAFFSV010000168         4121 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFFSV010000168 DAFFSV010000000
VERSION     DAFFSV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.169"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(424..741)
                     /locus_tag="LR110_002724"
     CDS             complement(424..741)
                     /locus_tag="LR110_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL4823646.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(742..996)
                     /locus_tag="LR110_002725"
     CDS             complement(742..996)
                     /locus_tag="LR110_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL4823647.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1355..1759
                     /gene="tnpA"
                     /locus_tag="LR110_002726"
     CDS             1355..1759
                     /gene="tnpA"
                     /locus_tag="LR110_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBL4823648.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1776..2924
                     /locus_tag="LR110_002727"
     CDS             1776..2924
                     /locus_tag="LR110_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4823649.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(3147..3365)
                     /locus_tag="LR110_002728"
                     /pseudo
     CDS             complement(3147..3365)
                     /locus_tag="LR110_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3631..3825)
                     /locus_tag="LR110_002729"
     CDS             complement(3631..3825)
                     /locus_tag="LR110_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823650.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(3925..4011)
                     /locus_tag="LR110_002730"
                     /pseudo
     CDS             complement(3925..4011)
                     /locus_tag="LR110_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT         1402 a          720 c          672 g         1327 t
ORIGIN      
        1 caacataata tttaagaagt ttcccataga tatattggac ttcctctttt aaaacacaaa
       61 aataaatact accttattta ggtagtattt attaaaagtt acgtcaattt tgattaaaaa
      121 tatactactt gaacctctca tgagtaaagt cacgaaattt ttgggaatag agcgtacttg
      181 atatcttatt ttttcactaa tagcagtgtc tcttattcac caagcgatcc ccgtagttcc
      241 taaggttctt aatttttctc tagactaatg attgtatctt tagaccttcg gccataacat
      301 ggagcaaaat aaaaaggtac taagtcttat atatcaatct tcgagtaacc gacggttaac
      361 aaccatatcg agcggcatta atcacccacc tgtagaggat gagcgacttt ttcctaatta
      421 ttgttaaaag cctagtttca tttgtttaga ttggaaaata cgtcctacaa gtatctcttt
      481 tttttcatgg tcgatccgat aaaataaggc atagcttttg ccgatcacga tccgatatgt
      541 tgggatatcc attccataga tttcggaaac ttcctcatgc atagttggaa aatttttggt
      601 tagttctaaa gccttctgga tcgcacctac aaatcttttt atgctggctg gagaaagagc
      661 aagctcattt tcccattctt ctattatcgt ttcaaggctg gtctgaaaac ttttagaata
      721 aactagcgtg tactctttca tctattcact ccagccgtat tttccgaaag cttttaccat
      781 ttcaatttct gaaactgtta aacctttctc aaagtcttgg aaactttctt caatcaattt
      841 gcgatcgtgt tcttttgcta tttgagtagt atctaatatg aataagttgt cttcttgttt
      901 ggcttggata atgtctccgc tttttaatcc aagagagctt ggaaggatca ctcccacgct
      961 attgccgatc tttctaacct ttagttcttt tcccatgtta tcacactcct tttagttttt
     1021 gatgaataaa ttataacatg ttataacaac aattgccaac ggtgtttctt aagggattcc
     1081 actccatatt attattggac aaaataagcc acttagaacg ctgaaaacat atacaaacga
     1141 tatcaaaata cgagtgaact actcaccata cacttccgtt aacatccatt taacagaatt
     1201 aggcagaagt cgcccatcct ctataggtgg gtgatgaatg ccgttcggta tgagggttac
     1261 cgttggtaac tcgaagaccg acaactaagg cgtattgcct ttttattttg tcccatgtta
     1321 caatcagtct atacaagaaa tgagttgata taacatggaa ttagatacaa ataatcattc
     1381 agtatttctt ctttattacc accttgtttt agtaacgaaa tatcgtagac aagtgattga
     1441 tgatgaaata tctgattatg ccaaaactac ttttgaaaga atttcagaat cgtatcacat
     1501 tactttagtg gagtggaatc atgataaaga ccacgttcat attatgttca aagctcaacc
     1561 taaaacagaa ttgacaaaat tcatcaatgc ctataaaagt gccagctcaa gattgataaa
     1621 acgtgacttt ccaagagtca aacaatttct ttggaaagaa atgttctggt ctaaaagctt
     1681 ttgtcttctg acaactggtg gcgcaccaat tgatgtgatt aagaagtata tccaaaatca
     1741 aggaaacaat cataaataga aagtaggtga actttatgga acaactaaaa gcatacaaat
     1801 tcagaatcta tccaacagag gaacaagaaa tcttctttgc caaatctttt ggttgtgtcc
     1861 gtaaggtcta caatctaatg cttgatgacc gaaaaaaggc gtatgaagaa gttaaaaatg
     1921 attcttctaa aaaaatgact tttccgacac cagctaaata caagaaagaa tttccgtttt
     1981 tgaaagaaat tgatagcctt gctttagcca atgcccaact taatttagat aaagcataca
     2041 agaatttttt tcgggataaa tccgttggat ttccacgttt caaaagtaag aaaaatcctg
     2101 ttcagagtta tacaacgaat aatcaaaatg gtacaattgc tttgattgat agtaaattca
     2161 tcaaagttcc taaattgaag tcattagtaa gaattaagct tcatagacaa ccaaaaggga
     2221 ttatcaaatc tgctacaata tcacgtcatt ctagcggtaa atactatatt tctttgttgt
     2281 gtaaagaaga aattatcgaa ttacctaaaa ctaattccac aattggtatt gacttaggta
     2341 ttatggactt tgcgattctt tctgatggac aaaaaattga taataataaa ttcacgtcca
     2401 aaatggaaaa gaaactaaag cgtgaacaac gcaaattgtc taggcgtgct ttattggcta
     2461 aaaataaagg taccaatcta tttgaagcta aaaattatca aaaacaaaaa cgcaaagtag
     2521 ctagattgca tgaaaaagta atgaatcaac gtactgattt tctgaataag ttgagtacag
     2581 aaatgatcaa aaatcacgat attatctgta ttgaagactt aaacacaaaa ggtatgttgc
     2641 gaaatcataa attagctaaa agtatttctg atgtttcttg gtcaaagttt gtgactaaat
     2701 tacaatacaa ggctgactgg tatggacgaa aaatcatcaa agtagataaa tggtttccgt
     2761 ctagtcagat ttgttcagag tgcggacata aagatggcaa gaaatctctt gaaattcgag
     2821 aatggacttg tcctgtttgt catactcatc atgatcgtga tatcaatgct agtatcaata
     2881 ttctgaccga aggtctaaga ctacattcta tgggattggc ttagatccaa tcatcaagaa
     2941 ccgtaggaac tacggggata gcttggtaaa taagagacac cgctgttagt aaagaaatac
     3001 gctatcaagt atgctctatt cccaagaagc tcccacttca agcgttaaga accgtcaggt
     3061 ttagctaagt ggtgagtagt tcacgtaggt atttttatta tatatagatt taacatatgt
     3121 cggtcggttg attgatatag ctaaagttat aactcaaccg aagccgattc atctacacga
     3181 ataaagttgc gagtgttctc ggctaattca taaaaattgt tatgtgttcc atatagcaat
     3241 actggaataa ctttattttc ttgaatctca taagcaactc tatacacggt gaccgcatac
     3301 ttaaatccat tagaccaatt acctgataaa cctccagttt tttgctctta cttaaatgga
     3361 tcaatggcaa tttcatcaca aacctataga ggatgagtga cttctgccta attacgttaa
     3421 aaaatattga aagtaactct atgtagcaaa aaatatctag aatttcattt cctcaaaact
     3481 gatcgatatt gtatctaaaa atatatacta cctaaaatag gtagtatata ttaaaatatg
     3541 tgcttttgtt agaaaaatca atacataaat tatcatatct gtatatgtca tttaggtatt
     3601 tcagttatgt tataatagtt ttgctgtctg ctaccccggc ctaatctggc aaggggagga
     3661 ggagatataa tgaaatacct ttttgatttt gtttttgcaa tcaagccact tgcagatgta
     3721 gtgtgcagtt acctctgcaa atggcttgat tgcaaaattg ccaaaaaaga cagtaagcct
     3781 acccgttaat tctataatat agaaacgtaa aaaaaacccc atcagcctgc acgctgatgg
     3841 ggtttggtga tataatgaaa tacctctttg ttactcataa ttgtaccata attagtcacg
     3901 aatgcaacca atagaaaaaa ctacttagaa cggtagatca tcgctaataa caatgcattg
     3961 tccaccaaat ggattttgtg aagcattaaa tacattgtta ttaattgtcg ctgcttttaa
     4021 ctaggcctct ccccttctat tataaaagaa gatatatatc atttcttaat tacctactac
     4081 cttaaaccaa aatttaagta ctacctaatt taagtattac t
//
LOCUS       DAFFSV010000169         4116 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFFSV010000169 DAFFSV010000000
VERSION     DAFFSV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.170"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(225..1730)
                     /locus_tag="LR110_002731"
     CDS             complement(225..1730)
                     /locus_tag="LR110_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL4823651.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLAENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1727..2413)
                     /locus_tag="LR110_002732"
     CDS             complement(1727..2413)
                     /locus_tag="LR110_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4823652.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2606..4027)
                     /gene="gndA"
                     /locus_tag="LR110_002733"
     CDS             complement(2606..4027)
                     /gene="gndA"
                     /locus_tag="LR110_002733"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBL4823653.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1136 a          865 c          697 g         1418 t
ORIGIN      
        1 caatagaaaa ttccctattt ttatttatta tttttaaaca cgaatattac agcattttat
       61 tgttttttcg ttcatctaaa agatacttac atttaattaa cggatgttat tctatttaat
      121 tgttcgctag tttttttata atgacatccc tgaatttctc acttgcttgt attttgtacc
      181 ttattctttg ttggatatat aaaaaaacaa gtaaagcaca tgctttactt gtcttctgcc
      241 tttgcgatag gaataaagat tctaaaaatt gtcccttgcc ctactacact ttctgctaag
      301 atacgtcctt tatagttttc agccagttgt ttagcgatag acaaccccag accatttcca
      361 cccttcgttc tagctcttgc tttatccact cgataaaaac gattgaagat cttattaagg
      421 tcttctttgg gtattccttc gccaaagtcc tgaatcgcga tctcaaattc attcagagtg
      481 gaagaaatag agatatggac ttcttttcgt gtagttgaat actttactgc gttatccaat
      541 atgataatga ttaattgttc aaagtgatta cgataaattt gcagggtcac ttcttttgtc
      601 aaatcatcat ctaaagtgat cacatagtct ggatataata tcttgaaatt attataaact
      661 tgataggtga cttcttttcc tttagttgtt tcattcgcat aatgaacatc tacttgttct
      721 gctcttgata agtctagcat ctcttgaact aagcttttca tacggctgat ttcttgcatg
      781 ctggcgctta atgattcttc taatatctca ggatcatctt tcccccaacg atttagcaga
      841 ctaagatgcc cttcaataat tgcaacaggt gtccttaact catgagatac gtcttccacg
      901 aattgctctt gttgttcaat atacaaacgc atacggtcaa gcatctcatt gaatatttca
      961 gctaaatctg ccaattcatc atttgtgtcg atttcaggca tgtggatatc tgattgcgga
     1021 tctttacgta tagtatccat tgtatctctg agtaccttga gtggttttaa gaaataagaa
     1081 gataaaaaga aacctaaaat actacttaag atcaaagaaa caacttctaa aacaataaga
     1141 gtcaataata gcttatttcg aatatcatag aaagaagata attcatagaa gctttgcgca
     1201 tagccgatct tttctcttgt acctttagaa tatataggct ggatagataa aaagccagtt
     1261 tttccttcta gtgtaacgat cgttggaact ttacgtgtag tctgaatcag gtttcgactg
     1321 ttttcatggg ttttaaaaat caagttctcg tccaaatcat aaacataaag atccattgat
     1381 ggctgcccta gctcagctat aaaggaatcg atcttcatta agtttccttc gaccagtgaa
     1441 gtatgatcta cgacctcatc tccgtcattt gtcgctttcg tcagattacg atacgtattt
     1501 actaatgtca gttcttcatc cgagttagcc aaacgagaag ctacttctga aatcgtacgc
     1561 tctacattgt ttctttcttt tgccacaatc agattgatag atgatttata ggtaatgaca
     1621 gcgaaaattg taaacacaac gaatatgaag aaagaactcg caaaggccca cttgattgtc
     1681 aaggagggcc ctttcagttc tcttttttcc gattttttcg gctttatcac gagcgcatca
     1741 cataacctgt tccgcgtaca gtttggatat agctttcctc cccgggaacg tcaattttgt
     1801 tgcgaagata acgaatatat acgtccacta cgtttgtttc aacttctgtt tcgtatcccc
     1861 acactttatt tagtaaaacg tcacgtgcta ataccacgtt cacgttttcc attaaagtaa
     1921 gcagcaactc gtattctctt tttgttagtt caattacttc atttccgcga cgtacgacac
     1981 ggttttcttt ttcgatagtc aaatcgcgat aagtaatagt agtttgttta gccacatttt
     2041 tatcgccttc aatatcgatt cgacgcaaca atgcacgaag acgtgctaat aattcttcga
     2101 tagcaaatgg tttgacgatg tagtcatctg ctccgtggtc aagtccagat acacgatcga
     2161 tcacagaatc tcttgcagtc atcataatga ttggtgtatt tttgacttgg cgtacacgtc
     2221 tgcagacttc taaaccgttc aattctggga gcatcagatc caacaatatg gcatcccact
     2281 cattatttaa tgccgcatct aaaccagttc ggccgttgta gtggacttct gcgttatacc
     2341 cttcatgctt caactctagc tcgacaaatc gtgccaagtt cttttcatct tcaatgatta
     2401 gaatattgct catttattat tttatccttt ctattctact ggctttttaa aaactctatc
     2461 attataccga taatatgctt aaaaatctat agtcatttta tcatggatag caattttaaa
     2521 aaagagctga agtctactca taacaatcga cttcagctct ttcgtcctct ttttattttc
     2581 tcatttccta acagccgaaa gactattatt cttcatggta ccatgagtaa tggaagattc
     2641 cttctttgtc gacacgctca taggtatgtg cgccaaagta atcgcgttgg gcttggatta
     2701 tatttgcagg cagacgttct gctctgtatg aatcgaagta agcgatggct gatgagaaag
     2761 tcggtacagg tacgccagct tggacagcca aggcaacgac gtctctgact gattgctgat
     2821 attttttcgt gatttcaacg aagtaatcat ctagcaataa attttcgatc tcaggatttt
     2881 tgtcataggc atccgtaatt ttttgcaaga atcgagctcg gatgatacat ccagcgcgcc
     2941 agatttttgc gatttcacca aatggcaagt cccaatcaaa ttcttttgaa gcgacacgta
     3001 attgagcaaa tccttgggca taactcatga ttttactgaa atacaacgct tctcggattt
     3061 tttcgaccaa ttcttttttg tctcccgtga actcaaaatc gttcgtacga gacaaaatct
     3121 tgcttgcttg tacgcgttct tctttataag cagaaatata gcgggcaaat acagattcag
     3181 tgatcaatgg aagtggcaca cctaagtcaa gcgcgctttg gcttgtccat ttacctgttc
     3241 ctttattccc agctgcatca aggatcacat ctactactgg tttacctgtt ccttcatcgt
     3301 ctttacgtgt cagaatgtca gcagtgattt ctatcaaata gctgtctaat tcgccttgat
     3361 tccattcttt aaagatatca gccatttctt ctactgaaag gtttaaaata ttcttcatta
     3421 agtcataaga ttcagcaatc agttgcatat ctccatactc aataccgttg tgtaccattt
     3481 tcacataatg tccggctcca tttgggccaa tatatgtgac acatggtgct ccgtcttctg
     3541 cttttgcaga aatttgctct aagattggtg caactagcgc ataggcttct ttttgtccac
     3601 caggcatgat tgaaggtcct tttaaagctc cttcttcacc accggaaacg cctgtaccga
     3661 taaagttgat gccagagttg gctaactctt cattacgacg catcgtatct ttaaagaatg
     3721 tatttcctcc gtcaatcaaa atatcgcctt tatcaaggtg aggaagtaac tcttggattg
     3781 ttgcatctgt tgccggtcct gctttgacca ttagcaagat ccgacgtggt ttctcgatag
     3841 attctacaaa ttcttcgatt gtatatgttg ctttgaaatt tttatctggg tgttcttcta
     3901 ctacagcggt tgttttagcc cctgtacgat tgaataaagc cactgaatag ccgcggcttt
     3961 ctatgttcaa ggctagattt ttacccataa cggccatacc gacaacgccg atttgttgtt
     4021 ttgacatatg aactcctcct acatacggga tttctcccta atctcttaca ttatagctta
     4081 acacggctac attgcaaact aaagaacaac atattt
//
LOCUS       DAFFSV010000170         4074 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFFSV010000170 DAFFSV010000000
VERSION     DAFFSV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.171"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(77..1051)
                     /locus_tag="LR110_002734"
     CDS             complement(77..1051)
                     /locus_tag="LR110_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL4823654.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVYESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(1500..2915)
                     /locus_tag="LR110_002735"
     CDS             complement(1500..2915)
                     /locus_tag="LR110_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL4823655.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESISAEMRYISKQREK
                     NEREKALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLNSFEKLSDGSYRLSSADF
                     VDLYQKASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDVSA
                     VVAENKKLKEENRSLRRRLTQLKNAFQKSITRLSIRFGLVEKDMGISEELKILDQEKT
                     LASQQNAPERNIGRSENEMEW"
     gene            complement(3137..3499)
                     /locus_tag="LR110_002736"
     CDS             complement(3137..3499)
                     /locus_tag="LR110_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823656.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKK
                     SQMSESKFTSMFNDVSLR"
     gene            complement(3503..3850)
                     /locus_tag="LR110_002737"
     CDS             complement(3503..3850)
                     /locus_tag="LR110_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823657.1"
                     /translation="MVAVLEMRCNMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERMMDVLNSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQI
                     GVIYRNKSKGKEL"
     gene            complement(3921..4061)
                     /locus_tag="LR110_002738"
     CDS             complement(3921..4061)
                     /locus_tag="LR110_002738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823658.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYIYAVAESSGEA
                     ND"
BASE COUNT         1153 a          895 c          576 g         1450 t
ORIGIN      
        1 aaaaaaacaa ccacaacgta atcacctgtt aaatttacca accaattttt cttaccaaaa
       61 tacttttgtt tttctgtcat ttttcttcct cctctgttac gtctagccct agaagccatg
      121 tgtctaattc tttttttaaa tcgtctgccg attctgcaaa ggtttctcgt aaatgattcg
      181 tcaattcatc attttgactt tcttgcttca gcaaatttcg atatttatat tttgtctttc
      241 gattaatttg cccccaataa tcatgttctg ttgttaaagc agtaatcatc attttgtcag
      301 ctacacttgt atcagcaggc aagtaatgag gactagcaaa actatctaag ctttcataaa
      361 ccatagcatg ccaatcagtc gctttccctc gtcttaattg taattctaac cgccaccaac
      421 tcacgatttc ttttggcaca atttttcttt tcttctcttg ttccagtttt ttattataca
      481 tccgaatttg acgttcagaa gagcgagatc cccaatatgc cgtctctaat tttccattcc
      541 gtccataaat aggcttaaaa gaaactggat caactacccg atattgtgta ataaactcat
      601 caggtacatc gataatgtcg caagcaatat ctgcacgtga aaagtgaggt tcgataaaca
      661 aatcatgaat aaaatttttc atggaactag ctaagaattg actaatctta ttcggattga
      721 agtctatacg ccctttcccc tcttgccatt ttaaaatttc aatataagca atattctctt
      781 catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa ccgttacctt
      841 catttaggcg cataagctgt tcaaaatcta aaatcaacac atcatgtgta gtgtgataat
      901 agatattctc tttaagattt ccaacaatgg taattcggtc gattgaccac tgaattttta
      961 ggggggtgtc cataaaaggg acacttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aatttagttc ctcctcaaag aaaaaagaga
     1081 ttggtggaaa gaattttcca gagttcacgt aactctaaaa aattcataac cagcaatctc
     1141 tagtcttgaa gaaacctgaa attattcgta taatgaattt gagttacgga gctagtgtga
     1201 ttagtttccg taaaggtcga gaacgtgtcc agcgttgctc gactttattt ttttgctctt
     1261 ttttcagttt atttatttga taactcaata atagcacttc tctttagcta tgcaaatatc
     1321 caaaaacata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaagtt
     1381 ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacagaccg cctccggtac cgttgactgc accgtataac
     1501 taccactcca tctcattctc agaacgccct atattacgtt ctggggcatt ctgctgacta
     1561 gccaacgttt tctcttgatc taatattttt agctcttcag atattcccat atctttttct
     1621 acaagcccaa aacgaattga taaacgagtg attgattttt gaaatgcgtt tttcagttga
     1681 gttaaccgcc gacgcaaaga acgattttct tccttcagct ttttgttttc agcaacaacc
     1741 gctgacacat caaccttttg ttgatttttc aaaaaagaat tttcttgttc taacgccatg
     1801 tttttatcaa ataaagcgat atttttatct aatgtatttc caagcgattc attcgccata
     1861 tcagcatatt ttttgctgtt cgtagcatta gacgcttttt gatacaaatc tacaaaatca
     1921 gcactagata agcgataaga accatctgat aatttttcaa aactattgag tggcacagga
     1981 tctttttgaa atacaacagc cagctctttt tccaaatcga taatcgattt tttctcttct
     2041 tcaattttcg ccttctcaga agccaccaga gccttctcac gctcgttttt ttctctttgc
     2101 ttagaaatat acctcatttc cgcagaaatg ctctcacgct cttttacagc cgtttggcgc
     2161 aattcagaaa tttcactcat gatttctttg tattcgtgaa tatctttaat tttgttggta
     2221 ccgacaatat cccttttcca accgtaatca ttcattaatc gttctataga gtcaacttct
     2281 tgatttctaa aattattgaa aagcactcga ccattatctt tatcagcgtg tatcccttgt
     2341 tgaaccagtg ctttattaaa gctcggctgc ttttgaacac cccgtttata accttcagca
     2401 accggcacaa tattcaagtg caggtgcgga cttatttcgt cattatgaat aacagcatta
     2461 taaacagcca aattagggtt tctctcttga aactccttgt aatactgaac caaaatttta
     2521 ttggcaatca ctcgattttg ttcagcttgt tgtttcgata tgccagtaga agaaccatct
     2581 gctcgatttg tctggaaatc atctaaaatg cctatttgca caataaattc tcgttgaggt
     2641 tccaactttt tatccttgcg cactttcgct aaatagtcct cgatttttct atcggctctt
     2701 ttttgtttcg cattatattt ttctaccact tcgccaaaca attcctcata tttatctgct
     2761 agattcgtct gctctaaata aatattttca tccgatttcg tgaagtcaat atgcttatgc
     2821 caatcattgg ttttctggct atcagttaac tcacgattat tatgagaaat acttgtattc
     2881 ttcgttcctt ttttaaatga aatactaaac cccaagttaa aaccctcctt cgcgctgtcg
     2941 ctaataaaaa gagtgtaaac ctttctgtta ctctttgtca aagagtaata ttttcctaat
     3001 tgtgacatgg ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca
     3061 acgaaaaaag gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt
     3121 cacgcaaaat aatcgcttat cgtaaactca cgtcattaaa cattgacgta aatttactct
     3181 cactcatctg acttttttta ctcggtatca ctccgagtaa gccgtcagat tcattgggat
     3241 aatctcgttc gcttggcgct gatttttcat cagcaagcca ctcattatcc actgattttt
     3301 cgtgttgctg atcgtcagaa attgacgata accaccgctc aaaatcatca aaagattcaa
     3361 atccaaaatg cttccgaagt gccttaccca tttcagcatt ttccttctca atcaatcgtt
     3421 ttttctgttg acgaagtttt gcctgttctt ttttcaattt ttcttcttgt gctttaatat
     3481 ctgctaaagt cattcccaag atttacagct ccttgccttt tgatttattt cgataaataa
     3541 caccaatttg atttaattgc ataacataag ttaaaatatt cagccaaaat tccttttcag
     3601 attcatcttc tgtttcggct attttaccgt aaacaaattt atcagtaatc aaagaagaat
     3661 ttgctaggcc tacattgatt gtagaattga gaacgtccat cattctctca agcatttcac
     3721 tataattatc ttcatccggc aaattcaaat aatcagctaa tcgttcatac atcaattcaa
     3781 ttctatcaac tgtctcttct cttaaatcaa ttttatccat gttacacctc atttccaaaa
     3841 ctgccaccat ttccgattct ttaattttcg atacacctaa ttcatcagca agatttttta
     3901 tggtcatctt ttctgacaaa tcagtcatta gcttctccac tactttcagc taccgcataa
     3961 atatatttaa cggcgtgctt agggataata atcggagagt caattttatt atttttcaaa
     4021 atcaattcat attgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DAFFSV010000171         4047 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFFSV010000171 DAFFSV010000000
VERSION     DAFFSV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.172"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(68..610)
                     /locus_tag="LR110_002739"
     CDS             complement(68..610)
                     /locus_tag="LR110_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBL4823659.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            745..1134
                     /locus_tag="LR110_002740"
     CDS             745..1134
                     /locus_tag="LR110_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823660.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1138..1902
                     /locus_tag="LR110_002741"
     CDS             1138..1902
                     /locus_tag="LR110_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL4823661.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2031..2978
                     /locus_tag="LR110_002742"
     CDS             2031..2978
                     /locus_tag="LR110_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL4823662.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3108..3848
                     /locus_tag="LR110_002743"
     CDS             3108..3848
                     /locus_tag="LR110_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823663.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1349 a          687 c          815 g         1196 t
ORIGIN      
        1 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
       61 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
      121 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
      181 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
      241 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
      301 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
      361 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
      421 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
      481 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
      541 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
      601 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
      661 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
      721 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
      781 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
      841 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
      901 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
      961 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
     1021 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
     1081 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
     1141 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
     1201 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
     1261 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
     1321 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
     1381 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
     1441 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
     1501 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
     1561 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
     1621 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
     1681 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
     1741 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
     1801 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
     1861 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
     1921 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
     1981 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
     2041 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
     2101 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
     2161 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
     2221 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
     2281 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
     2341 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
     2401 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
     2461 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
     2521 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
     2581 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
     2641 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
     2701 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
     2761 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
     2821 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
     2881 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
     2941 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
     3001 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
     3061 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
     3121 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
     3181 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
     3241 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
     3301 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
     3361 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
     3421 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
     3481 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
     3541 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
     3601 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
     3661 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
     3721 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
     3781 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
     3841 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
     3901 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
     3961 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
     4021 tcgcagatcg tttgcaaaaa tagttta
//
LOCUS       DAFFSV010000172         4030 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFFSV010000172 DAFFSV010000000
VERSION     DAFFSV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.173"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            180..776
                     /locus_tag="LR110_002744"
     CDS             180..776
                     /locus_tag="LR110_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL4823664.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            900..2699
                     /locus_tag="LR110_002745"
     CDS             900..2699
                     /locus_tag="LR110_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL4823665.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2977..3189)
                     /locus_tag="LR110_002746"
     CDS             complement(2977..3189)
                     /locus_tag="LR110_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823666.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3651..3800
                     /locus_tag="LR110_002747"
     CDS             3651..3800
                     /locus_tag="LR110_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823667.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1182 a          689 c          712 g         1447 t
ORIGIN      
        1 ttttaaaaaa aacaagttat taaataagtc tttagttgga tgtttttttg agatcaatct
       61 tttatactta tgatgaactt acaaaattgt ttgttttctg tcatatcaaa aaatggcgca
      121 gttagttgct tttctagtaa agtatagtta acaaagaacc tctaaggagt tgatgaataa
      181 tgaatattgc tgcctctcga aaactgataa atttcatatc cattatcgga ttaggcctat
      241 caatcgcgct gacgatttat tttattaatt taggtgtttt caaagatttg aatgcgttaa
      301 gaggattagt aggtgattcc atcattttag gaccgattat ttttgtcttc atccaaatcc
      361 tgcaagtagt tattccaatc attcctggag ggatcagtac tgcagctggt gtcctgatat
      421 ttggccctta tgctggattt atctataact atattggaat ctgcattggt tcgattatca
      481 tttttctgct agggcgtcgt tatggaaagc cattcatact ttccatgatt agcgataaga
      541 catataacaa atatattggc tggctggata atcaaaaccg ttttgagaag ctgtttgctc
      601 tagctatttt tcttccagtt gctcctgacg atgcgttatg tttgatggct ggattgacaa
      661 acatgtcagt gaaacgatat acactaatca ttttgattgc taaaccactg tcgattttct
      721 tatatagtat ggccttgatt tatggaggtc aatatctaag tggtcttctt ggataatatt
      781 tattttgtaa aattagtcat tgattgacaa tgttaaatct gggaaagggt ctgcaaaaca
      841 cactgcaccc tttccttttt cattagattt gcgctacaat gtgaagagca ggtgatagga
      901 tgaattattt gaaacgtagc ttaggtaatg cttgggactt tttaaaagga acacaagctt
      961 atttccgtga tgtcttgctg atgcatggct ttattttatt tatttgttta cccttattga
     1021 gcagtacgac ccgttttatt ttacaccgag gggcaattga ctatttgtct tatgacaata
     1081 ttccttcaat tttttctcaa catcctgggg ttctactctc gttaatcgta gttttgatga
     1141 cgattttact gttagtttat ttcgaattta cttttctttt gatgagtgtt ttttttatta
     1201 aaaagaaaga accaatctct ttgaagcaac tcttacactt gacgatactt cagctgaaga
     1261 aagtgagacc cattactttt ttgtttttcc ttgcttattt tttattgatt ctaccgatca
     1321 gcggattaag ttttaattct gatctactgt caaaaataaa aatccccgct ttcattatgg
     1381 attttatttt tgctaatcgt tggatcatcg tcagttcctt tttactcgtt tatattttcc
     1441 ttggctatat tgggattcga ctgatttttg ctttaccgga aatgattcta cgggatcgtc
     1501 cttttcgagc agccattcgg gaaagctggt cattgaccaa atcgcgtctt ttggccatca
     1561 cgggccaatt tattgtgatt ggcggtacga ttttactcct ttcttcttta ggatatatcg
     1621 ttgttatttt ggcacaaagt atggttgaac aatttttccc agactatgca ttgatcagcg
     1681 ctgtgtttgc tatgacgctt ttacaaggga ttttattatt caatatcgtg atgtctaccg
     1741 tagggatttt ttatatcatt gttgatttca tggatgacga aggcttcttg cctgagatcc
     1801 ctaagtggtt tatcccacaa gcccccaact tgcgtttctc tgcgctgaaa aacactggtt
     1861 tgactctttt tgcagttttt ttcggtattg gcgtctgtct ttacaatatg gattatctga
     1921 cttcagctgt tcagacaaaa ccagtgactg tctctcacag aggtgtgagt acccaaaatg
     1981 gggcgcaaaa tactttagaa gcacttgaaa aaacaagcag agactatcat cctgattatg
     2041 tagagatgga tgttcaggaa acaaaagatg gtcatttcgt agtgatgcat gatgcaaatc
     2101 ttcgtcactt aacaggggta aatggaacac cacaggatct tacattgaaa gaattgacaa
     2161 atcttacagt tactgaaaac ggagtgcaag cccctgtttg ttcttttgat tcttatttgg
     2221 aaaaagcaca taagctaaac caaaaattac ttattgagat caagacctct aaaaaagatt
     2281 cggatgacct tgtcgaacga tttgtccata aataccgcaa agttatctta gagaaccatc
     2341 atattttgca aagtttgacg taccaaaccg tttcagattt aaaagaagca gaaccaaact
     2401 tttatgtggg ctacatctta cctttcaata ttgttgggcc ccctgtgacg cctgctgatt
     2461 ttctgacaat ggaatatagt acgatcaacc gtaacttcat taattctgcc caccaagacg
     2521 gaaaagaagt atatgtatgg acaatcaacg attcagatgc tatgagtcgc atgatgtttt
     2581 atggtgtgga tggtatcata accgatgaga tgggactact aaatgaaagt gtgaaacaaa
     2641 cagatgaaga aatcacttat tcagataaac tgctaaattt tgttttggga gtcggctaat
     2701 caaaaatcgt taatgaaaaa agtctttata caacaaaaaa cgcataacct gccaccgcag
     2761 gtatatgcgt tttttgtttg acgctcataa tgtgtgctta actttctctt gattccttca
     2821 tcgcacgaac aatagctaaa atagcaggaa ttgcagagat cactgcaaga ataattccca
     2881 ttttccctga actttttctt tttggcatat cttctcgctc ccttctgtct ttatttatat
     2941 tataagggag ttcttgcgaa ttctcaaaaa tactgtttaa gaaaccaatt cagcaggtgc
     3001 gctaatatca cagaatttta cgagcatgcg atacttgctt tcattgataa gttgatagtc
     3061 tcgttggtca taactttcaa catacacgac agccgtgttg atatatgtac gttcaataca
     3121 gccgattagt tcatgagata ctccgatcgg tttgcatata taagatcttc ctacttctgc
     3181 ttttaccatt tcattccctc ttttctttat ttttattttg ttttttcttt attttataat
     3241 aacaaattac cttgtatttg aaaacgtttt tatccatttt tttatttttt attttgagta
     3301 tctttctgct ttttagataa ctatttttta acacgatctt agacgttgtg acaaaaacgt
     3361 ttgtttatat tcctatgttg ttcgtgcgtt tttttcttta tattgctaag ttatttataa
     3421 agaggtgagc atatgattta tattcaagca atagtatttg taaccttgat gattgggcta
     3481 cttatcggat taaaagaaaa tagctaaacc atttaagaaa aaataaataa ggaaaatgat
     3541 aaatgctttt taattgcatc aaaaaacgta cgatgctaaa cttggtataa gttcgaaagt
     3601 ttaaaggaaa ctttcaagaa ctaaattaca ttacaaagga gatgaatttt atgacgttta
     3661 caaaaacaaa tatgcaatac cgcgtagctt ttgatacaac ttctaaccgt tttatggcga
     3721 ttgatgcaca aaacgaaaat aacgttgcgt ttggtattac aatcgaacaa gcagtccgag
     3781 cattaaacga atctagataa ggagtgaacc attccaaatg gcgactgatg agatctcgga
     3841 aaatcaataa aagacgtttg taacggtaat ccgcgacaaa cgtctttttg tattcattac
     3901 atatatttgg aaattgtctc tctttatctc tagacctaaa caaaaacaca agttctaata
     3961 tcaaccatct cgttttttat gacaattttg ttagctgttt cgatgataat attccgttat
     4021 tatctgtata
//
LOCUS       DAFFSV010000173         3725 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFFSV010000173 DAFFSV010000000
VERSION     DAFFSV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.174"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            44..919
                     /locus_tag="LR110_002748"
     CDS             44..919
                     /locus_tag="LR110_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823668.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1064..1216)
                     /locus_tag="LR110_002749"
                     /pseudo
     CDS             complement(1064..1216)
                     /locus_tag="LR110_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1484..2815)
                     /locus_tag="LR110_002750"
     CDS             complement(1484..2815)
                     /locus_tag="LR110_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4823669.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(2964..3677)
                     /locus_tag="LR110_002751"
     CDS             complement(2964..3677)
                     /locus_tag="LR110_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL4823670.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
BASE COUNT         1275 a          694 c          558 g         1198 t
ORIGIN      
        1 tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata attggcttga
       61 gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg gttttgtccg
      121 tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg aagattataa
      181 ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat atgccataga
      241 aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac gtccaataaa
      301 aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta gttcctctct
      361 tgtacgggag cttttaacag agattcttat agaacatccg caatttatcg aacgcttcaa
      421 agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta tcatccataa
      481 atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca tcatggagat
      541 gcgaaagttc atccaagaag cagttgaatc gctcattcag caaaatgcct atcttcccgc
      601 attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg agcctgagga
      661 tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat tattagcaga
      721 agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag tcgatcatac
      781 tgacgcctct tattcaaaac gctactccat aaaaatcctg aaagaagact ttcgggaaaa
      841 agaattttct aaccaaaaga aaaaaataga taaaaaaact aaagaaacgg taaagaaaga
      901 cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca caagaaatac
      961 agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa gacttaacgc
     1021 gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag tcatgacaaa
     1081 tttccttcgt tgctgggtag attttccggc attgttgata gattttatga tacttttcta
     1141 cattttgtaa gagaggttcc actgcatctg tatattggat aaaattctga caacaacttt
     1201 caggtgtgtc gaaccactct aatctttaaa gataaatatt aaaataacga ttaataatat
     1261 taacagtctt tatttttcac caccaaaaac ctatctttgc atatactaaa tagacgtata
     1321 tacacagata ggtttgtctt gtgaaaaata atgattatcc ttaccaaaaa atgtttgttt
     1381 ttctacatgt tcgtacttat caaaaaatat gatccttatt gaaattactg gctatttata
     1441 ttccaacgac ttcttgtgtt aatttttgtt atctcatagt aaattatgtt tcattttcac
     1501 ttaatttagt tgtcacgtgt tcgtcaattc gtaaaaacgg taaatatata aacacacttg
     1561 cggctataat taatatttgt aaaacagctg ctcgccagtc tccggctgtt gctaaaaatc
     1621 ccgatataac tggtggcgta gtccaaggaa tttgcactac tacatgatta attaatccca
     1681 cagcagttgc aaaatatgct actgtcaaag aaaaaatcgg tgcaagcaca aagggaataa
     1741 tcagactgat attaaataca ataggcaatc caaaaataat aggttcgctt atattgaata
     1801 tagcgggagt taccgataat ttagcaaagt ccttataatc ttttctacga ctgaagataa
     1861 atattgcaat caataaagca atcgtatttc ccgatccacc cattaccgca aaggtatctt
     1921 taaaggccat attgataata tgtggaattt gctctttgtt tgcataagct gccatatttt
     1981 cttgcatatt ctgaatcaaa aacggatcta acaatgaacc tgatattact gcttgatgga
     2041 taccaaatcc aaaaaataga ttagcaatag atgtaattaa caaaaatcct ggtaaaccag
     2101 ttgtaatttt acttaatggc tctgtaacta tcgtggtaat tagcttattt atatccatat
     2161 taaacaattg aatgattata aatgacaata aagcaaacga ggtaatcgta ataatgattg
     2221 ggattaatac attaaaagac tttaaaacag cgggaggaat atttccggat agctgaatct
     2281 gtagtttctt attattagaa agtttaataa ataattctgt agccgctaat ccaacaaata
     2341 tacctacaaa cattcctgaa gctcctgtat aggatatagg tatcacaccc gtcacttcaa
     2401 cagctttatc cgcattttca ggagtaaaca tcatctgcat aggtgttaat acaacaaatg
     2461 tcgaaatagc aactaatacc gctgcaatat gatttgtata atttctgctt gcacacatat
     2521 gataagatac tgctgctaca actaataatg tagttatgct taatgtacca ttggcaattg
     2581 aagtaccaat ttgttgtaac gtggtcaatg tactagtatc gacaatttcc cccatgaatc
     2641 ctgttggttc taagatcact gaattaataa gtgtcacaaa tcctgctaac atcataaatg
     2701 gcataaatgt agcaaacgca tctcgcaaag ttcttaaatg gatctgattc cccaacttag
     2761 tcgcaactat accaaatttt tcaaatacaa tgtccttcct gctacttttg cccatatttt
     2821 ttctccttta tgcttattgt aatcgattac tttttccacc caaataatac atcacttatc
     2881 aaaaaaagta aatactttta attaaatata tatatctttt ttagtataaa aaaataagct
     2941 ctgaaaaaat aattttcccg agcttatttt tcaacaaaaa cagcttcaaa aacaaagtct
     3001 tgatataaat gtctcgatac cgaatactcg aaaggtttac cagacgatag agcaacaact
     3061 ttgtctatct caatcataaa ttcacctgga tctattttta atatttttgc gtcaaattca
     3121 ttcgcagtat ctcctttgat acgtatgtgc gaagtcttta tttccagacc cagttcattt
     3181 gtgatataag catatacaga tttctttaaa tgttttggtt ccaagcctgg tatgactgct
     3241 aatggcatga atgtttgctc aatcgagtat ggttgacaat caataaaacg gcttcgtaca
     3301 atattatata catagtcttt tacactgata cttaatttat cagctacttt tttcgatgga
     3361 agttcaattg agaaagtatg gatttctgaa tctacgtgtt cttcaccaac cacgctaaac
     3421 aaaccttcgt ttggaccaag aactcgtgag ttatcaattg gtgttctgac gacttcagtc
     3481 ccatacccac ttctacttcg caaaactcct tcggaaacaa gtaagtctac gcctttcttt
     3541 actgttaact tgcttgtatg ataaataact gccaattcat ctcctgtagg aagtttctca
     3601 ccaattttat actctccatt tacaattttt ttctttatat ctaagtaaat ttccatatac
     3661 ttcgcaattt ttttcaatgg tgaccctcca atcaaaaaca tatcttcatc tatactttaa
     3721 cataa
//
LOCUS       DAFFSV010000174         3719 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFFSV010000174 DAFFSV010000000
VERSION     DAFFSV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.175"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(369..926)
                     /locus_tag="LR110_002752"
     CDS             complement(369..926)
                     /locus_tag="LR110_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823671.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1105..1716)
                     /locus_tag="LR110_002753"
     CDS             complement(1105..1716)
                     /locus_tag="LR110_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823672.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(1829..2086)
                     /locus_tag="LR110_002754"
     CDS             complement(1829..2086)
                     /locus_tag="LR110_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823673.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2683..2961)
                     /locus_tag="LR110_002755"
     CDS             complement(2683..2961)
                     /locus_tag="LR110_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823674.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(2995..3246)
                     /locus_tag="LR110_002756"
     CDS             complement(2995..3246)
                     /locus_tag="LR110_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823675.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1129 a          757 c          499 g         1334 t
ORIGIN      
        1 atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct tttatgtatg
       61 ctgttagttg attagatggt catttgttga gctaggtcgt ttattttaaa caagttgttt
      121 ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa tgcatacgca
      181 tctgtatttg ccactttgat tcgtataaag ctaacaacta ttaactcctc tcagctaata
      241 gttgctctct tttttattca gattcatttt tgctgatcac agcctagtat ttcaatggtt
      301 ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa tggattagta
      361 gatttcgatt attcaaagcg attttctatt ggtggatcgt gttcccaact aataagacgg
      421 attccaaacc tatcgtacag atcttctaaa ccacttataa attttgcacc tatttcaggt
      481 gttttattgc gtggatattc tattgttacg ccattaaatg gataccaatt aggattaggc
      541 tctctttttt tctgtacatc tcccatagaa tagttagcac tttctgggac ataatatatt
      601 cttcgaccat cctctaattc atctaataag atatttcgtg atcgtctatc acttgctaaa
      661 tgtagacgcc acgtgcgata ccatgccatg ctacctgcat ctgcagaaaa tccatcaaat
      721 aatgcaacaa ttgtatcttc tatcacaatc ctttccccat ctacttcata ttctaatcta
      781 aaatcaaact cccctcgggt aatttctggt cttggtggct taggacccaa tattattcta
      841 cttaaaaccg cattcgttcc cagatgaaac acagctataa caacaataat gattaatatt
      901 ttgtttctat ttgataaatt tctcattatt cgcacatcac ctctacattc tcacatatta
      961 gttcgtaata ctcctttaat atcaaaatat ctactggtcg taataatatt cgctatgaat
     1021 tcctactact cgtataagaa aggccaaatc atcagctttt acttgctgat gatttggctt
     1081 gtttctattt atcgtaattg ttttctaatc accaaaattg ttaacaatcg gttcagatat
     1141 ttcccagttg attaaggtaa ttccgtattg tgtcaaaact tcttgcgctt ctccgaaatt
     1201 aagtacagtt cttatgtatc cactttctcc taatataaag tctacctctc ttttaaaacc
     1261 ttcctcgaga tttctaaggg ctatatgtgg ttttaatgac attgattgta cattttcccc
     1321 catataatat tgcgcacttc ctcgactaaa atagattcct agatcgtccc caaaaggtag
     1381 atcttctcta ccactagcta atctacttct ccatcttcgt tctcttttca tagaacctgc
     1441 gctgaactca cacccgttaa actcagctat gacagtatct tcaattatgt taatctgtcc
     1501 atctagttcg tattcaatcc taaatggaaa ttctccatag gtgatttccg gttcgggtat
     1561 tttaggtgag attaatgcaa agaaaggaaa tattaagctt ggcaacatca aaaaaactcc
     1621 tcctatgatc acaattcccg atgtaatcca aaatataatt tgtacaattc tcattgatcc
     1681 attgtattcc attttattcc ccctattact attcatatcc gcctatctat atcgatcttc
     1741 caatgcagag ataattgctt ccataccttg attttttact gagttaccta tgcgagatat
     1801 cattctccac taaggtgcat catattgtct atctaacatg attaattctc caataggtgt
     1861 atactcctcc aaaaacaaca tttaatgcat ctccctcgac aacataagct accatgtttg
     1921 ctgtatatgt gtttgagtct aattcataag cagataaatt tactgtcgct ggattaaata
     1981 taatcgcatt cgtatttata gctactgaat ttgctgcttc agcaccacct tttgaatgtc
     2041 cgatcattgt gatttcaata gtaggattta cgtggacagc cctcaccgtt taccctgacc
     2101 attatagaaa tcgcttactt ttttgaacta cgcaacaaag gatacaatgc tcctaacttc
     2161 actgtctcca tttttgtcca ctaaggaata taaaaaatag gcattaattc atttaattcg
     2221 gtcacaacac gttacctttt aaatcattat actgtatctc atacattatt atcaataaac
     2281 tattataaaa atgcaaaaaa tgctttatca atctattttg taattggtca taatatcatt
     2341 gaaatcatac cctgtatcga tttttcctat gaatctcgtc tatttgtgat tgacgaaaaa
     2401 gaggcaagac agacactgga atggtaattt tacccactat tttgctccaa ctatttgtaa
     2461 tcgtacccac ttttgaaata ccgagatgat cattttaatt gatatgctga ttcccaagca
     2521 aaaccccaag aaattaaatg ctctatcgca tctagcttct tgggttttgc ttatataaat
     2581 tccaacattt aaatttttta tgctgttcgg tctcagaaat gccgtttttg cttatatcac
     2641 aatatcaaga tgtcaaatta ttcgtgttgc cgatgacttt gattaatttt tttcaaattg
     2701 tttattaaga ctaatgctgt ctttttgcag ataccaatgt tcgaactttt taacattatc
     2761 atatgatcct gcaagtttta acttcttacc attagatgtt atcaaagtta taattaacct
     2821 tgcctttttc ccattaggat cttttatttt atgaattttc tgcaggtaat ctctcctaat
     2881 ggcagctatt tttacctcat cagtgctgat aaaccattga tcatccacat acatactgcc
     2941 tgttatgttg tgtttttcca tttcttccga gaacgaaaag tttaaggctc gttcttactt
     3001 ccaaagttga atgagtccat ggattggact tagtgtgaga attgagaaca acaataagaa
     3061 tccatatcct cccgcaaaca tccctaaggc tatactgtct ctccatccat aagtaggatt
     3121 agaagagaaa agagtcaccg tttgtaataa tatgaaaagg aataatccag cacaaacacc
     3181 aaagataaca actccaaaca ttgaattttt atacaatgac catttattta taggcttttc
     3241 tttcatttta cttcccccta ctgttctgtt cacacggaat gaattcaaag ttcataagcc
     3301 ctcctctttg aacatatgaa tacgacttgc taactatact ttaaaaaagc ttgttttaag
     3361 cggttatagg cgagttgtaa tctctattca tctcattagc aaataacttg ccgaaaagtc
     3421 tactgaaatt aaagcagata gtggaagaat tatttagcca ggatagacac tatttgtgaa
     3481 acacatgtct ttacttttga aattctatga tgaaaagcct tggaatacag tagctcttaa
     3541 ttggtaatac tttttccatt ataaataacc tcttttcatt ttatatttat cccctctaaa
     3601 aataatatct aatctaatat tttaaactta tatttccctc gctgtgctaa tagagcatta
     3661 ctaaataaat agaaactaga aaaaagcatt acgcctgcca gaataaacca attatttgt
//
LOCUS       DAFFSV010000175         3699 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFFSV010000175 DAFFSV010000000
VERSION     DAFFSV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.176"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            62..2503
                     /locus_tag="LR110_002757"
     CDS             62..2503
                     /locus_tag="LR110_002757"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBL4823676.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2833..3537)
                     /locus_tag="LR110_002758"
     CDS             complement(2833..3537)
                     /locus_tag="LR110_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823677.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1183 a          653 c          814 g         1049 t
ORIGIN      
        1 ggaacaagag ctatttgttt ttacagcaaa gaatggtata tttttgtagg aggtggctta
       61 cttgaaagaa gtcgttatga tagatgctgc aagaacgccg ataggaaagt atcgcggaag
      121 tttaagtcca tttactgcag ttgaattagg cacacttgtg actaaaggat tattagataa
      181 aacaaaactg aaaaaagata aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg
      241 taatggtcaa aatgttgcca gacagattgc tttgaacagc ggtctccctg tagatgtccc
      301 agcaatgacg atcaatgaag tttgcggatc tggtatgaaa gcagtgatct tagcacgcca
      361 gctgatccaa ttaggtgaag cagaacttgt gatagccggc ggaacagaga gtatgtctca
      421 agcacctatg ctgaaacagt atcagtcaga aacaaatgaa tatggtgaac caatttccag
      481 tatggtcaac gacggattga ctgacgcatt ttcaaatgca catatgggat taaccgcaga
      541 gaatgttgca acacaatttt ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc
      601 ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt
      661 caagatttct gatgaagacg tgttatctga ggatgaagca gttcgtggaa atagtacatt
      721 ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa
      781 tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga
      841 aaataataat ctgccttatt tagcaaccat caaagaagta gcggaggtcg gtattgaccc
      901 aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa ttgacagata gatcaggaat
      961 gaatttatca acaatcgatc tttttgagat caatgaagca tttgcagctt cttctatcgt
     1021 agttagccaa gaactgcagt tagatgaaga aaaggtcaat atatatggag gagcaatcgc
     1081 attaggccat ccaattggtg caagcggtgc acgtatcttg actacattag cttacggatt
     1141 gcttcgtgaa cagaagagat acggtattgc ttcattatgt attggtggag gtttaggctt
     1201 ggcagtcttg ttagaagcga atatggagca aacacataag gatgtgcaaa aaaaaaagtt
     1261 ttatcagctt acgccatctg aacggcgcag tcaattaata gaaaaaaatg tattgacaca
     1321 agaaactgcg ttgatttttc aggaacaaac actttctgaa gagctctccg atcacatgat
     1381 cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa
     1441 tggcaagaaa aaatggattc cgatggcaac agaggaacca tctgtcattg cagctgcaag
     1501 taatggtgca aaaatctgtg gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg
     1561 gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg atcaatgcag ttaatcatcg
     1621 aaaagaagag ctgattcttt gtgcaaatga aagttatcct tcaatcgtga aacgcggggg
     1681 cggtgttcaa gatatctcta ctcgggaatt catggggagt ttccatgcat acttatctat
     1741 tgatttttta gtggatgtaa aagatgcaat gggcgctaac atgatcaact cgattcttga
     1801 gagtgtggca aacaagttac gggagtggtt ccctgaagaa gagattctat tcagtatttt
     1861 aagtaatttt gctacagaat cattggcttc tgcctgttgt gaaatcccat tcgaacgctt
     1921 agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa atccaacagg caggggaata
     1981 tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa gggatcatga atggtataga
     2041 agctgtagtg gcagctacag gaaatgatac acgtgcggtt agcgcttcga tccacgccta
     2101 cgcggcaaga aatggattat accaaggatt aacagactgg cagatcaaag gggataaact
     2161 agttggaaaa ctaacggtgc ctttggctgt tgccacagta ggcggagctt ccaatatcct
     2221 gccaaaagca aaagcatctt tagctatgtt agatattgat tcagccaagg aactagcaca
     2281 agtaattgct gcagtagggt tagctcaaaa cctggctgct ttgcgtgcat tagttacaga
     2341 aggaatccaa aaaggccaca tgggacttca agctcgttca ttagcgatct caattggggc
     2401 tattggtgag gaaatcgagc aagttgcgaa aaaattacgt gaagcagaaa agatgaacca
     2461 gcagacagct atacaaatat tggaaaaaat ccgggaaaaa taaaaaagca atatctttag
     2521 aagaataaag caagatttac agttatttta tttactgcag atcttgcttt tttctctatc
     2581 agaaaatatg tttattatta gaatttttta tagttaaggt taggcatctt aagaaatcag
     2641 gcaactccaa gatatcttca ggtagaagag aagtcgccta acattgtagg atcataggtg
     2701 atttgttgga caggtcaagc tgatatttga gcctgatgtt agaaggaatt tctttttact
     2761 tgctgtagaa attattatat gactaaaaag gctgggattt taaatcccag ccttttatga
     2821 caaatagaat ttttatcgat cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat
     2881 caaagtcaat ttgactttgt atttccgttc gagtgctttt tcgataacga actcattttc
     2941 agtagtcata gaaatagttg ctaatttacg aggttcatca gcattttttt cattaataac
     3001 tacttcatgt tcattttgtt ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa
     3061 atcagcaaaa cttaaagcgg cattcgtatc agttgagcgt gtattttcag ttgtgtccgc
     3121 ttttggtttt tccgtttttt cttctggcac actgactgtt tcttctgtaa ctggtgtcgc
     3181 aacagcttgc gcagttggtt catcgttttt caatcgttga tcattccagt aagacacgat
     3241 ttcaataaga agattgtcac ggaatcgttc gaaaatcccg ttttctcctg aacgaattct
     3301 ctttaataca gtattgaagt tgtcatcgat tggcacgttg atccgtgtat cttcatttgt
     3361 tttcccagct aatccgatcg ttaagatttg acggtttttg ggatcaagca tgacttgtaa
     3421 cggcaaacca taacgtcgca agctctcttc ttctagagaa cggaataatt gtcctcttac
     3481 accatccaat gaaggacgga attgatttaa taatttttgt ttctgttttt tcttcatatg
     3541 ttctctcctt cgctcatacc ctttcgatta taagaaaaaa acagccaaat gaaaaggagt
     3601 aatgcaaaaa aacttgcgta tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt
     3661 ttttgaaaga aaaataaaaa atggaacgga ttatgtata
//
LOCUS       DAFFSV010000176         3687 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFFSV010000176 DAFFSV010000000
VERSION     DAFFSV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.177"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            55..507
                     /locus_tag="LR110_002759"
     CDS             55..507
                     /locus_tag="LR110_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823678.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            521..1210
                     /locus_tag="LR110_002760"
     CDS             521..1210
                     /locus_tag="LR110_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823679.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1335..3686
                     /locus_tag="LR110_002761"
     CDS             1335..3686
                     /locus_tag="LR110_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBL4823680.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1255 a          478 c          770 g         1184 t
ORIGIN      
        1 ttatcttatc gtagatatat attataaatt taataagaaa gcgaggtgag aatcgtgaaa
       61 aaaacgatac taagtatttt ttcagtattt acaatattaa ttgggggcta ttttcttttg
      121 aatttagatt ctgttaattc aaacgctcaa ataaaagatc aaagagtagc tgaacataat
      181 aataaaaaaa tgagtttgac caaaaatatc tattctatgt cagatgaaga attgtataaa
      241 gaatttggtg atttatccgg ggaacagtta gaagaaagta ttccaggaat tagtgactca
      301 gataaaattg ttgtcttgtc tgctggtggg caattaatgg gagatggcaa atccgaatta
      361 gttagttata aacaaaatca cagacagttt attgatgtta atactgattc caacgctatt
      421 tcgattaagg atctaaaaga gcttttaaat agtaaaagaa atgaattaaa gcagataaaa
      481 gaaactaatt cagtggaatt agagtaaagg aagattcatt atgaaaaaga tttttggtgt
      541 ttctgttggg ctacttatga tgttaacatt tggaaatgca gtatatgctg ctgagaaaga
      601 agttatttca gatccagagc agtacgcctc acgatacttt gctgattttg aagaagaaga
      661 taaagtactt gcattgccag aaggtggttt tttaaaaggg gaagctaaaa ttgtatctat
      721 ggataatcca gatataattc aaggttcata taattcagaa actgatcctg cttcagtttc
      781 agtgacagaa gcaaaaaaaa ttgttgctga aaaattcaaa aatgatattg aaactactcc
      841 aattatgcct cgaggagcat ttccacccga tgttggtact agcggacacc ttcttgtact
      901 agcaggagga ggaagctata aatcacagcc atttagtggt gcgggatggc gttttggagg
      961 tatgggtgta aaaccagctg atggtacagg caacttttta cgttggaaat cctacattga
     1021 cgatggaaga gtaggaaatg taaatcaagc caacgcaact aaatctggtt ctattcaagg
     1081 aatggcattg tatgctcgtg aaggattcaa atactttgca ccaaacgggc ctggttggac
     1141 aatgaatata ggtatttatt acacttataa tcctgttgca ggtacgtatt atgttgttca
     1201 aaatatttaa taaaacagca caaagagatt tctttgtgct gttttattat ttttagttgt
     1261 tgacaaacac taaaatataa aatataatca acacataaac gaacgtatgt tcggcaagaa
     1321 agcaggggat ttttatggat gagatggttt taagtactca gaaatggcta aacaagaaat
     1381 acagtaatgt tacagggttt gataaggttc cagaaaatgg gcgaacaggg tggcctacaa
     1441 tctatggact tattgaagga ctacaagtag aacttggtat tactaatcta gtagctaatt
     1501 ttggacctac cactgaaaaa atgtacgata atcaggttac tcctaaatgg ggcaaaaatc
     1561 tacctaaaaa tattgtattt cttattcaag gtgccttttg gtgtaagggg attaatccag
     1621 gtggcttcga tggggtatat actccatatt tagatactgc tgtaaaagag ttgcagactg
     1681 atgcagggtt taatggaagc gctgtaactg gagttttgga tgcgaaatgg gccaaagcgt
     1741 tatttgatat gagtgctttt gttcttgttc ccggcggaga tagtaaaatt agaaaaatgc
     1801 agcaatggtt aaatgtaaat tatttagaat acacgggaat aatgccttgt gatggtattt
     1861 atcaaagaaa ttcaaatcaa gcattaattt ttgcattaca agctgaacta ggatattctc
     1921 ccagtgaagc aacagggtct tatggtaatg ggacaactag taaaacttat cctgtgtctg
     1981 aaggtaactc gggaaactat gtaagaataa ttcaatatgg attatatgtt aatggttatt
     2041 ttgaagacgg tacattcgat ggtattttca ctaaatatat gggtttagaa gtactggcat
     2101 tccgaaaatt tatggtattg cctgaatata ctgaaatagc agatgttgtt gtaataaaag
     2161 gactattgtc tagcgctgga gatattagaa gatctgcaga tggcgcagat acttctacgc
     2221 aattaactag atctcaaatc caaacattgg ttgataatga tataaaaata gttggtagat
     2281 atcttactgg tactgttgga attggaaaag atgaacgaaa taagtatcta actacagagg
     2341 aattgaataa tattttttct aaaaatttat ctgtgtttcc tatctatcaa gatggaggtg
     2401 cagcattagc ttattttact tatgatcgtg gcttatcaga tggtaagaag gctatagatg
     2461 cagcaaaaaa tctaaatata ccattaacaa cagttattta ttttgctgtt gatctagaca
     2521 tgttagggga agaaattctt gcatatgctg gagaatattt taagggggta tctgctgtaa
     2581 tgtcatatag cgggtatcaa acgggtgttt acggaacaag aaatgtttgt tctcaaataa
     2641 tcaataacgg gtatgcttcg tttgcatttg tttcagatat gtcgactggt tatagtggga
     2701 atttaggttt tcctatgcct agacaatggt cttttgatca atttgttgaa tttaccatag
     2761 gaagtggcaa cggagctgtt ggtatcgatt taatcgctac atcgggtaga gatagcggat
     2821 ttaatgagtt atcaaatgat actaataatg ataattacat tgccaaatac aatcagaagg
     2881 ttataaatca gatggtacgt gcgtatcaat atctatcaca agctggtctt gataatcctt
     2941 ggaaccctca tttgacattt tatagatatg taaattattc tggtttgtca tgggacatca
     3001 tttcttctcc tgtaacagag catgatagaa aaatttatga tgaatatagg aataaattaa
     3061 ctaatagcga gggcctgtat aattatttta tagatcctaa tactggttca ataataggtt
     3121 tgcctcactt aattgtgacg ttacaatcac agatgtttct agccgacaca atcaatgata
     3181 gtgtttctga ctttgcaggt tggttaggag atttaatgac ttgctggggt gaagttaaga
     3241 aattaggaat acaaatggag caaggtgtat ttgcattagt aggtacggct gcaggtggaa
     3301 catttagtct agaagattta taccaagatg ttgacgcggt aaatgtttat accttagcaa
     3361 aaagaaattc tgattcaaca gctttatcgg ctttaaaaac gtactattgt gataagggtg
     3421 ctattgatag atttaatcaa tttgttaaga acagatttgg ttcttcagat aaaatcgctt
     3481 cagtaacaaa tagtttatta gaaaatgacg aaacagttga tatcttaggt gtagggataa
     3541 tttcattttt cattgataaa atagttaata attcacttgg gacagatttt acttatcttg
     3601 attttattgg agatgagggg aaagttgcaa atggattttc tgaaaaaatt attcaatttc
     3661 aagaaaatag aagtaaactt aattaag
//
LOCUS       DAFFSV010000177         3629 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFFSV010000177 DAFFSV010000000
VERSION     DAFFSV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.180"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            147..1376
                     /gene="arcA"
                     /locus_tag="LR110_002762"
     CDS             147..1376
                     /gene="arcA"
                     /locus_tag="LR110_002762"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBL4823681.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            1467..2486
                     /gene="argF"
                     /locus_tag="LR110_002763"
     CDS             1467..2486
                     /gene="argF"
                     /locus_tag="LR110_002763"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBL4823682.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            2601..3548
                     /gene="arcC"
                     /locus_tag="LR110_002764"
     CDS             2601..3548
                     /gene="arcC"
                     /locus_tag="LR110_002764"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBL4823683.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
BASE COUNT         1252 a          642 c          770 g          965 t
ORIGIN      
        1 cgcttaaaaa tagaaaaacg tttaattatt taattatcga atattaatgt gaggaatgtc
       61 tcattttgaa tgagacaaaa ttatgaaagt gattacaaaa ggaggatatc ctatacatgt
      121 aatcaaaaag aaggaggcaa cacaacatgg ataaacctat tcacgttttc tctgaaatag
      181 ggaaattaaa gacagtttta ctgaaacggc ctggacaaga agtggaaaat ttgacaccag
      241 atattatgga tcgtctgttg ttcgatgata ttccatatct tccgattgct caagaagaac
      301 atgacaactt tgcgaagaca ttgcaaaatg aaggcgtcga gactctttac ttggaaaaat
      361 tagcggcaga agcgattgac gcaggcaatg tcaaagaaca attcttgaat aaaatgttgg
      421 atgaatctca tattgcatcg aatgctgtaa gagagggtct tcatgagttc cttctatcaa
      481 tggaaacgca agaaatggta gataaaatca tggctggggt tcgtacaaaa gatatcgaag
      541 tacgttctag cagtctatat gatctgtcag cagacgatga ctatccattt tacatggacc
      601 caatgccaaa cttgtacttt acacgcgacc cttccgcatc aatgggaaat gggatgacag
      661 tcaataagat gacttttgaa gcacgtcgtc gtgaatcgat gtttacagaa tatattttga
      721 aacatcatcc aagatttgca aataaaggaa tcgaagtatg gttggatcgt gaaaatccag
      781 atcatatcga aggcggagac gaattgatct taagcgataa agtagtagct gttgggattt
      841 ctcaacgaac taatgcgaaa gctttagaaa aattagcacg ccacctattc gcaaaaaatt
      901 caggatttga aaaagtgttg gcaatcaaaa ttccgaacaa ccgtgcaatg atgcatttgg
      961 ataccgtatt tacaatggta gatcacgata aattcactat ccatccagca attcaaagta
     1021 aagacggtaa gatggatgtc tttacaatcg aaccagatgg tgatgatatc aagattactc
     1081 attcagataa cttacacgaa acgatgaaag ctgctcttgg attagatgat cttgtactga
     1141 tcccaactgg taatggagat gagatcgttg cacctcgaga acaatggaat gatggatcaa
     1201 acacattggc aatcgctcca ggtgttgtag tcacttataa ccgaaattat gtatcgaacg
     1261 aattgcttcg tagctatggc atcaaagtgt tagaaatcaa ttcaagtgaa ttgtcacgag
     1321 gtcgtggtgg tccacgctgc atgagccaac cacttgtccg tgaagacttg aagtaatttg
     1381 tttcctgacg gcagtcttac tgtcagctgc cgtcgggatt tcctctttaa tcttaaataa
     1441 aaacaatcta aaggagaatg tttaatatga aagaatcagt attccaagga agaagcttat
     1501 tagcagaaaa agactttaca aaagaagaat tacaatattt gatcgatttt tcagaacact
     1561 tgaaagactt gaaaaaacgt ggtatcccac atcactatct agaaggaaaa aatattgcgc
     1621 ttttatttga aaaaacatct acacgtactc gttctgcatt tactactgca gcgatcgatc
     1681 taggtgcaca tcctgaatat ctaggtgcaa atgatatcca attaggtaaa aaagaatcca
     1741 cagaagatac agctaaagta ctaggacgta tgtttgacgg aatcgaattc cgtggattca
     1801 gtcaaaaaat ggtagaggaa ctagcagaat tctctggtgt tccagtttgg aatggcttga
     1861 ctgatgaatg gcatccaaca caaatgatcg ctgacttttt aactatccaa gaaaactttg
     1921 gaacagtaga aggtatcact gttgcttact gcggagatgg tcgaaacaat atggctaact
     1981 ctttattagt cacaggtgca attttaggag cgaatatgcg tatcgttgca ccaaaagaat
     2041 tacaacctga agaagaaatc gtcaaaatgg cagaaggctt tgctgaaaaa tccggtgcac
     2101 aattgatgat taccgacgat gttgataaag gtgtagacgg agcagacgtc ctttattctg
     2161 atgtatgggt atcaatgggt gaagaagata aattcgaaga acgtatcaaa ttgttgaaac
     2221 cttaccaaat caatatggaa atggtagaaa aaacacacaa cacagatcgt ttgatcttct
     2281 tgcactgctt gccagctttc catgatacaa acactgttta cggtgaacaa atgaaagaac
     2341 gctttggcat cactgaaatg gaagtaacag atgaagtctt ccgcagcaaa tatgctcgtc
     2401 aatttgacca agcagaaaat cgtatgcact caatcaaagc gatcatggca gcaacattag
     2461 gtaacttgtt tatccctcgc gtttaagcat aaccacattc atctcacgaa aaaacgagtc
     2521 agcattgagg gaaaatcaca aaacgctggc tcgttttgtt atctagaaaa ttcggtagaa
     2581 ctgaagaaag gacgaatgaa atggccaaac gaaaagtagt tgtcgcttta gggggcaatg
     2641 ccatcttgtc tacggatgca agtgcaaaag cccaacaaga agcgttaatg gaaacagcaa
     2701 agtatttagt gaaatttatc gaacaaggag atgaattgat catctcccac ggaaatggtc
     2761 cgcaagtcgg taacctattg attcagcagc aagctgctga ttctgagaaa acaccggcaa
     2821 tgcctttaga tacatgtgtt gcaatgacag aaggatctat cggttactgg ttacaaaacg
     2881 ccatgggcga agtactgaag gaaaaaggga tcgataaaga tgtggtttct ttagtcactc
     2941 aagttattgt ggatgaaaat gatccttcat tcaaaaatcc aagtaaacca gtcggtccat
     3001 tctacacgga agaagaagcc aaagaacaaa tgaatgctga ctccactgtg acattcaaag
     3061 aagatgctgg acgtggatgg agaaaagtag tcgcttcccc taaacccatc tcgattaaag
     3121 aagcacgtgt catcgaaacg ctggtcgatc aaggagttat cacagtctct gttggtggcg
     3181 gtgggattcc tgttgtagaa acagctactg gattagaagg acgagaagcc gtaatcgata
     3241 aagattttgc ttcagaaaaa ttagccgaaa tcatcgatgc ggatttgctg atcgtattga
     3301 caggagtaga caatgtgtat gtcaattacc aaaaaccaga tcaaaagaaa ttagaaactg
     3361 ttactgtttc agaaatgaaa caatacattg atgaaaagca attcgctcca ggaagcatgc
     3421 ttccgaaagt agaagcagct attgcatttg ttgaagcaaa accaaacgct aaggcaatca
     3481 tcacttcttt agaaaatatc gagaatcttt tagcttctga agaaggaacg attatcgtag
     3541 ctgattaatt tgttaagtct ttcgtattta cgaaatgatt tatagctatc caggtggtat
     3601 ttcttctcct ttaggccatc tggattttt
//
LOCUS       DAFFSV010000178         3621 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFFSV010000178 DAFFSV010000000
VERSION     DAFFSV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.181"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            262..1044
                     /gene="rpsB"
                     /locus_tag="LR110_002765"
     CDS             262..1044
                     /gene="rpsB"
                     /locus_tag="LR110_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBL4823684.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1143..2024
                     /locus_tag="LR110_002766"
     CDS             1143..2024
                     /locus_tag="LR110_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBL4823685.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2160..2882
                     /locus_tag="LR110_002767"
     CDS             2160..2882
                     /locus_tag="LR110_002767"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBL4823686.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            2885..3442
                     /gene="frr"
                     /locus_tag="LR110_002768"
     CDS             2885..3442
                     /gene="frr"
                     /locus_tag="LR110_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBL4823687.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1339 a          614 c          791 g          877 t
ORIGIN      
        1 gaaaaaggat aataaagaaa caaagagaaa tctactgttt ttcttgaatt ctttttatct
       61 ttcaggtaaa ctattgaatg ttgagcagaa aagtatattt gctcttgcga tacggatctt
      121 ttgtctgtat aatagctgag ggtgctttat gcatcagcaa atccacattc ttattggatt
      181 gtcggtaggt gcttgaaaaa gtttgccggc aaggtagaag ataagaagag gaaaaataaa
      241 accaaatcgg aggaaacaaa catggcagta atttctatga aacaattact agaagccggc
      301 gtacactttg gtcaccaaac tcgtcgctgg aatccaaaaa tgaagaaata tatcttcaca
      361 gaaagaaacg gaatctacat cattgacttg caaaaaacag ttaaattagt agatgcagct
      421 tatgattaca tgaagagcgt tgctgaagaa ggcggcgtag cattattcgt aggtacaaaa
      481 aaacaagcac aagaagcaat caaagaagaa gcaactcgtg caggacaata cttcgttaac
      541 catcgttggt taggcggaac attgacaaac tgggatacta tccaaaaacg tatcagccgt
      601 ttgaaacaaa tcaatgcaat ggaagaagat ggaacttttg aagttctacc taaaaaagaa
      661 gttgctggtt tgaacaaaga acgtgaacgt cttgaaaaat tcttaggcgg tatcgctgat
      721 atgccaagaa tcccagatgt aatgtacatc gttgacccac gtaaagaacg tattgctgtt
      781 caagaagcac acaaattaaa tatcccaatc gtagctatgg ttgatacaaa ctgcgatcca
      841 gatgaaatcg acgtagtaat cccttcaaac gacgatgcaa ttcgtgccgt taaattgatt
      901 acttcaaaaa tggctgatgc tttcatcgaa ggaaaccaag gtgaagatca agtagttgaa
      961 gaagatttca cagaagaaaa cacagcaaca tcaatcgaag aaatcgtaga tgttgttgaa
     1021 ggcgacaatt cttcagcaga ataattttga atgatggagc tgtttcaaag gctgggcgac
     1081 aagcctcact ctcctttgaa gcagcttttt ttaaaaagaa aaacacacag gaggagttaa
     1141 acatggcaga cgttacagct aaaatggtaa aagaactacg cgaaatgact ggcgtaggaa
     1201 tgatggatgc gaaaaaagca ttagttgaag tagaaggcga catggaaaaa gcagttgatc
     1261 ttttacgtga aaaaggaatg gcaaaggctg cgaagaaaaa tgaccgcatt gcagctgaag
     1321 gattggcttc agttgctgtg aaaggtaaca ctgcagcaat cgttgaagta aactcagaaa
     1381 cagactttgt ttctaaaaac gaaatgttcc aagatttagt aaaagaaatt gcagaacttg
     1441 ttgctgaaaa caaaccagca gacatggaag ctgcaatgaa aatcaaaaca gataaaggta
     1501 caattgaatc tgacttgatc gaagcaactc aagttatcgg agaaaaaatc agcttccgtc
     1561 gctttgaagt tgtagaaaaa gacgacaatg ctgcatttgg tggatacttg catatgggcg
     1621 gacgtattgc cgtattgact gttctagatg gaacaactga cgaaacagtt gcaagagacg
     1681 tagctatgca cgtagcagct atcaatcctc gttatgtaaa cgaatcacaa attccagaag
     1741 ctgaattaga acatgaaaaa actgttctta cagaacaagc attgaacgaa ggcaaaccag
     1801 cgaatatcgt tgaaaaaatg gtagaaggac gcttgaagaa attcaaagca gaaatcgctt
     1861 tggtggacca accattcgtt aaagatcctg atatgactgt tgaaaaatac gttgcatcaa
     1921 aaggcgcaac tgttaaaaca ttcgtacgtt tcgaagttgg cgaaggtatc gaaaaacgcg
     1981 aagataactt tgtagaagaa gttatgagcc aagtgaaaaa ataattctgt tgaataacag
     2041 acgggaacgc acggtttgtg cgctcccttt ttttaagcaa aacaagaaaa accagtctta
     2101 gagtgtcgaa atcatctaag tatggtaaaa tggcaataga aaatattgga ggaatgaaca
     2161 tggtcacacc gaaatatcag cgtgtagtct taaaattgag tggagaagct ttagctgggg
     2221 acgcaggatt tggaatcaaa ccaccagtaa tcaaagaaat catccaagaa atcaaagaag
     2281 ttcacgagct gggcgttgaa atggcaatcg ttgtgggcgg cggaaatatc tggcgcggac
     2341 aaatcggagc acaaatgggc atggaacgtg ctcaagctga ttacatgggt atgttagcta
     2401 cagttatgaa tgccttagct ttacaggata cgttggaaaa cgtgggtgta ccgacacgcg
     2461 tgcaaacttc gattgaaatg cgtcaaatcg cagagccata tattcgccgt aaagcagaac
     2521 gtcatttaga aaaaggacgg atcgttatct ttgcaggcgg taccggcaac ccgtacttct
     2581 cgacagacac aacagcagca ttacgcgcgg cagaaatcgg cgcagatgtt atcctgatgg
     2641 ctaaaaacaa tgtagacggt gtttattctg ctgatccgaa agtagatgca aatgcagtaa
     2701 aatttgaaga actgactcat ctggaagtta ttgccaaagg attacaagtc atggattcaa
     2761 ctgcaagttc actaagcatg gataatgata ttccactgct tgtcttcaat ttaaatgaac
     2821 acggaaatat tcgccgtgca atcttaggcg aaaatatcgg aacaactgta agggggaaat
     2881 aaaaatggca gatgcaatta tgacagaagc aaaagaaaaa atgaaaaaag cagcacaaaa
     2941 tcttcaacgt gaacttgggc aaatccgcgc aggccgtgcg aacgctagcc ttttggaccg
     3001 tattactgtg aactactacg gtgcgccaac accattgaac caaatggcat caatccaaat
     3061 tccagaagca cgtgttttaa tgatcacacc ttttgacaag agcattcttc aagatgttga
     3121 aaaagctatt atggcaagtg atatcggaat cagtccaacc aatgatggaa atgtcattcg
     3181 cttagttatc ccgcaactga cagaagaacg cagaaaagaa ttagctaaag atgtgaaaaa
     3241 agaagcagaa aatgcaaaaa tcgcgattcg taacatccgt cgtgatgcga tcgatgaata
     3301 taaaaaacaa caaaaaaatg gtgatattac cgaagatgat ttgcgcggat tagaaaaaga
     3361 cgtacaaaca ttgacagata acagcatcaa agaagtagat aagatcgctg ctgataaaga
     3421 aaaagaatta ttggaagttt aataaaatca taaaaacaga ggggcggatc attccgcttc
     3481 ctctgttttt atttatattt cttttattta aaaattaaaa aaagaaaaaa ttattcagtt
     3541 tttaaataaa aaaacaatga tttcatagga aaaacagtta gatattgcta taatacttaa
     3601 gatagtggga atcttttaag t
//
LOCUS       DAFFSV010000179         3602 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFFSV010000179 DAFFSV010000000
VERSION     DAFFSV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.182"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            298..612
                     /locus_tag="LR110_002769"
     CDS             298..612
                     /locus_tag="LR110_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823688.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(665..1438)
                     /locus_tag="LR110_002770"
     CDS             complement(665..1438)
                     /locus_tag="LR110_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823689.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLNVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            1543..1788
                     /locus_tag="LR110_002771"
     CDS             1543..1788
                     /locus_tag="LR110_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4823690.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            1944..2795
                     /locus_tag="LR110_002772"
     CDS             1944..2795
                     /locus_tag="LR110_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HBL4823691.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            2828..>3602
                     /locus_tag="LR110_002773"
     CDS             2828..>3602
                     /locus_tag="LR110_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBL4823692.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSR"
BASE COUNT         1341 a          481 c          618 g         1162 t
ORIGIN      
        1 taatcttgtt gtttctacta gtaatgtatc tttttggaaa ctatgtaacg atgtaaacaa
       61 gatttttttc agaagaaaaa acgataaaag ttgtgataaa cgatttaaaa aataggagaa
      121 aatatatcaa gcgcatggta tgaaacgcac atttaaaaag attcgactaa ctaaaaaaat
      181 aagtttacaa agcattgttg tgtattacaa gtgatctata cactgaaaaa tgatagagaa
      241 aatctgatac gtccgcaaca atggacaatt gaaaatgaaa gtgaggaaat tggaagaatg
      301 gctgatgaaa ataaagttgt tccgcttgaa acactagtga taaataagga agagtttgag
      361 caactttttc ttgatttttt tgttcaatat gacaggattt atgattttag ttcgtcacta
      421 gatttatttg aattagaggc agaaataata gaaaacagtc gagaggagaa tggtaaaatc
      481 atcatttcag tagaaaaatt caatgaatta ttctcagaaa catttgaaat agatacagat
      541 tattatcaac cacaagaagt tttccaatca tttagaaatc agattattcg ctcaggtaag
      601 accgtaaact gaaaaacaat aaaagaaacg ttgaaagaat aattattcct tcaacgtttc
      661 ttttttagaa tgttttaatg accaaagcag tttcattgtc aggcttaggt aattcatgaa
      721 atcccgtatt tatagtgctc aattgatcaa ctaaccatgt tttacttttt gaattgtaga
      781 taaatctaaa attaatatcg ttattatcgt gttgaagggg cgtctccaaa taatctgctg
      841 tatattcata gtcaacgtaa cctgctaata caaaagagag tatcccttta tttaatgtta
      901 gatttaatgg ataatcatac gactttattt ttttcaactt catattgtac atatcttcag
      961 ccataaaagt tgagttcgtg atttcatctt gggtctcatt aaaaaagtta tccgaataat
     1021 cgcttatttt tgaaaagtct ttattggcac cagcctcaaa aaattgttca actttttcag
     1081 tataaagttg tgctaacgct cctttttcaa tattaacttt aatattttca tgtctatcaa
     1141 tttgtgtgct gttcagtaag ggaagatcag cttttagttt tatatcgtct tgatttcttt
     1201 caatttcaat tggcaaaatc ttttcagtag attcagtttc tgataaagga acatttagtt
     1261 ttttatcatg ataataagct tcaaatccat ctggaaccgg aaaaaaatgt attgggtaat
     1321 tcttttctga ggttgttgta ctagtttccg tacttgtaga acttgtgtca gaagtggtac
     1381 acccgcttat agttccgatg agtaaagcgc aagttagaat gattgcaaat ttcttcaaaa
     1441 aaattcctcc ttaaaattga atatggacta atactagtcc cattgtagca taccgaatct
     1501 ttaagattaa taagaaaaag gactaatttt agtccaaaag gtgtgctatt cttgaaagag
     1561 gaaaaaatta ttgataaaga aaatgtaatc ggtagcaata ttagacgagt ccgattagaa
     1621 aaaggaattg gtcaaaccga gctgattaga gacctacagc ttcgtaaaat tgctataact
     1681 agggaaacgt tggttaagat tgaaggtggc cgccaacaca ttaagttgga tcaattgaaa
     1741 gccatcaaag atattctaca ggtgtcttat gaagatttgc ttcaatgact acttgtagtc
     1801 atttcgtttg aaattattat gttacaccct tatgtgtcac gaagaaatac tggtataata
     1861 atgaatggga tatgtaacga tagggtatac tgttatgttg cctaataaat tgtggatcaa
     1921 aatacaagaa tggagggata tccttgtata aggcatatga attggatttt acaaatgctg
     1981 acttaaatgc attctcctca agctcgcaat catatacaca tgtagtaaat caaatagatc
     2041 acaaccaaaa atctattaac aagagaatag aaaatctatt tgaaagtgaa aatgatttag
     2101 ttgattatta ttctaaggac agcaaaattc tagatgcaga cgaaattatt aaagattggt
     2161 ttccaactat aaaagcagat atttttatat ctcactctca tgctgatgaa aaattagcta
     2221 tacgttttgc atcatggcta tttgaaaatt ttggtcttac tgcatttatt gattcttcgg
     2281 tttggggata cagttctgat ttattaaaaa aaatagacca aaaatattgc tataaggaac
     2341 aaacaaaaac atatgattat gataaaagaa atgtaacaac tagtcatgtt catatgatgc
     2401 tttctacagc tttaaataat atgattgata gtacagaatg tctgtttttt cttaatacac
     2461 ctaattcaat tagtttgagc aatgaaatca ctaatgaaca gaaatttacg tattcgccat
     2521 ggctatatag cgaactgacg acagcttcaa tagtggaaaa aaagaatcca cgattggaaa
     2581 gtaatcctca aatgagcacc gaggatgtta gaagcattat aaagcactat tcttcgcaag
     2641 aaaatctgca aataaaatat tctttaaatt taagtgaatt aacttattta cgaaaagctg
     2701 atttgaaaaa atgggcttta gatgctgtgg aatgcactaa aggaagtaat acattagata
     2761 ttttatataa aaacaccatt ttcactaatc tctaatcaat ttactgaatc gaaaggaacc
     2821 gggtagaatg gataagaaag ttttaataaa gaggttctct gaagctattg aaaaaggaaa
     2881 tgcagcaata tttgcaggtg caggattatc gatgtcacaa ggatatgtaa gttggccgga
     2941 actacttaat gatcctgcaa cagaaatcgg gttggattct aaaaaagaaa cagacttagt
     3001 tactctggct caatattata aaaatgaaaa tggtggatca agaggaattt tgaatcagat
     3061 acttatggat aattttggtg aagaattaga aatatctgaa aatcatcgaa tactagcaag
     3121 tttacctatt gaaacttatt ggacaactaa ttatgatcat ctaatagaaa aatcaatccg
     3181 agaagcttat aaaaaccctc aggttaagaa aaattatact cagttagcaa caactaatcc
     3241 aaatgtggat acaattgttt ataaaatgca tggtgatata gatgatgtca gttcgacagt
     3301 aataacaaga gatgattatg aaaaatatga tgatgattcg tacgcgttat ttaaagaaac
     3361 tttaaaagga gatttgttaa caaaaacttt tctcttttta ggatttagtt ttacagaccc
     3421 taatttagaa agaattttat cagatataag atgggttttg agagaaaacc aaagacctca
     3481 ttattgtata atgagaaaaa tattaaagga aaattttgtc gattctgagg atttttttga
     3541 tcaagagaga tataattatg aactgacaaa aagaagacta caaatcaatg atttgtccag
     3601 at
//
LOCUS       DAFFSV010000180         3553 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFFSV010000180 DAFFSV010000000
VERSION     DAFFSV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.183"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            920..1246
                     /locus_tag="LR110_002774"
     CDS             920..1246
                     /locus_tag="LR110_002774"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4823693.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1227..2162
                     /locus_tag="LR110_002775"
     CDS             1227..2162
                     /locus_tag="LR110_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL4823694.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            2791..3042
                     /locus_tag="LR110_002776"
     CDS             2791..3042
                     /locus_tag="LR110_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HBL4823695.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
BASE COUNT         1109 a          698 c          631 g         1115 t
ORIGIN      
        1 taggtttttt attaaaacta tcttgacctt tcgggagatt tcactgtgat ttatttttat
       61 tcccatactt ttttcgttgg tcactttcgt atacctaatc taagcgatca tcggcgacgg
      121 tgcttttaca acgaatggca ggacaaaaca aggggcggaa aaactttttt gcctatttca
      181 taaaaaactt tttcctacag caatcccttg ttttgtccta ccacacgttg tgaacccaaa
      241 gcacgccgac gaacgcttag ctatacgaaa atgacctcca aaaacaaaaa agtgaggtga
      301 atagaaacaa aaagaaattg aaatctccca aaatgtcgtc cgcctcggag acaacttgct
      361 gctgaaaatg gttggttaat tcatttaagt attaaataag ataaatcatc aaattaatcg
      421 gaaaaatagc atgattggcc ttatcaaaaa taaactgtaa aaacttatat ttctatgagt
      481 ggtggaacaa tgctaatacc aaattaattt atcgattacc cacctgcaca aaccaaagat
      541 agcattatta ggaaacgaat caatcagcga atccagcatt ataatgagga tactaaaaat
      601 aaagttttca taattataaa tccgccttcc tacgaaaggc accaatgagg tgatgaaaac
      661 cttagagcgc tttctttctt tttttattcc gtgttaccct ttccttgcag gtccctggta
      721 cactacagaa cccgaggagg tgagtggcgg ggctgtatag cggcaacccc gcataattat
      781 atggtgtcgg tcatccgtta aggcatcaat gaatggcgca aacccgtagc ccgtaaactt
      841 atctcttccg aagttgactc gatttcgccg gatgaccagt ccctaccaaa cctgtactat
      901 ttttttacag gaggttgcta tgttcaaaat ctttcgtaaa aattgttgtg gcttggatgt
      961 ccacaaaacg tggatctatg cgtgtatcgg aatcacagat gcgaatggtc gtacagtgta
     1021 taagcaagct cgcttctctt cctttacaaa aggtctcaat gaattagctg aatggcttgc
     1081 taaatacaac tgtaaggatg tgtgtttgga atcttccggt aaattttgga ttcctgtttt
     1141 taatattctt gaaaaaactt gttttgtcac actggctcat ccgaagtaca ccaaacccca
     1201 gaaaggaaat aaaacagatc gcaaagatgc aaaaatggat ttgtgatctc tttatgtgcg
     1261 acatgatcaa acccagtttt attcctggtc ttgaaatccg ccagcttcgt gatctaatgc
     1321 gttatcggac gaaactgacc aacatgaaaa caagtgaaaa gaatcgtgct ttaaactgct
     1381 taaccgtttc taatctgaaa ctagatgatg tttttagtga tgtgttcgga aaatcatcca
     1441 cttctatcat ccaatacttg ttagaacatc ccggtgaatc atttgatgtt cgaccattta
     1501 tccatgcccg ttgtaaaact cccgttgaag aaatccaagc ggcagttgag ggggcgattt
     1561 ctgtacctca agcgattaag cttcgccaat gcctcaacca tattaacgag attgagaagc
     1621 atcgagaaga gatcaatcta gagattcaag ctcttgcaga acccttctcc cctgttttgg
     1681 aactacttcg taccataccg ggcttagaca ctcagccaat aacagcgatt gcgattcttt
     1741 cagaaatagg accggatatg tccgtgtttc caacttcgaa acactttatt tcttgggctg
     1801 gatgctgtcc aagaaacgat cgcagtgccc gacgaacaaa atcaacacgt atctctcgtg
     1861 cagggcagtc gttgaaaccg ctacttgtac agattgccaa tgctcttctc agatccaaag
     1921 aacatccaga atttaaaaat cgctatcgta gaatcaaagc gaatcgtgga cacaagaaag
     1981 ctatcattgc tatttgtaag atgctcctga ccgctatctg gaatgtctta tcaaaggtgg
     2041 aaccctattc tgctaaagga tatctggagc caaaacctgc gaaagaagag aaagttctga
     2101 cgcaatccca agctctggac cttttaagaa aacgcggata taccattata gatagtgctt
     2161 aaaccaaagt tacgttgatt cttcctaaat agactcgttg tcttccattt caaaaatttt
     2221 gaaaacggta ggcttattta gtgtaccgtt cttcaggtat cttcaaatag atttattttc
     2281 aaacttattt cctttcttct tttactctcc tatagcttag catttaagtg ggcactcgtg
     2341 tcaccttgct agatagctat gaatgccgta ataagcctcc tagtatatag gcagactgcc
     2401 aattataact gattcaatcc acattatgat aaaatccacc ttttcgtttc atatgtgttg
     2461 aaatatcaca tatgtcaaaa gtgcacgagt cttttcattc agtgctttgt aaatactcaa
     2521 aattattcct tttcacttta aaaaaaagat aattagtaca aaatcagaaa tgaggaaagt
     2581 attaggatgg actagaaagg agttgttgaa tttgttgcaa cagattggtc atttatcaat
     2641 taaaaattag gtgaagtttt attcatcact gcagctgctg tttcccagag aataaaacgt
     2701 ctagaagaaa ctggtcagtt actgtattat caagcagcaa ttgactacga acaagcttga
     2761 ttactaacca aagcgtttat ccacctgttg attaaatcaa atcaaaaatc aggtttttat
     2821 acgttcataa aagcaatctc cagtgtactc gaatgtgact gtatcatagg tccttattcg
     2881 atgttaatca aaatggggtt tccttctacc gaatcattgg acagttttat caatgattta
     2941 tcaaagtttg gacgaaccaa cacccaaatc gttttctcga ctcttgtttc cttaatggtt
     3001 tatcagttct ggaatgttta tcatgcgctt ttattaaaat agaaaagagc ttttttcatg
     3061 cgacaaagca gtgatcattt attggcacat gcaatctttg tggatccatt ttttgaaaat
     3121 acgatattta gttttgaccc aaaaatcttt aaatacgtat ttgataattt atcttaacaa
     3181 ccagataatt ttattcagca ccgtattatc tatattgaat tctcggaatc aagataaatt
     3241 tttttccgtt tttgtttctt ttttaatctt cttcttgtta ttaatagtga ttaggatgat
     3301 attcctatat tgatctttga taattagata taatttttga ataacgttcc ctgctattcg
     3361 agctagaaat acaagtacgc catgacctag ttagcgacaa ctacttagag atttcttttg
     3421 ttctgggaca acaaaatgcc atgacaatag cagtaaataa tgatacttcc gtaaggctcg
     3481 ccgtaaactg gggagaggtg aaattcctat gtgcttaccc aaagcggttc aaaaatttta
     3541 aactattatt tta
//
LOCUS       DAFFSV010000181         3513 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFFSV010000181 DAFFSV010000000
VERSION     DAFFSV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.184"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..268
                     /locus_tag="LR110_002777"
     CDS             <1..268
                     /locus_tag="LR110_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823696.1"
                     /translation="IGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPS
                     RPPQSRNWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            278..481
                     /locus_tag="LR110_002778"
     CDS             278..481
                     /locus_tag="LR110_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823697.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            692..1276
                     /locus_tag="LR110_002779"
     CDS             692..1276
                     /locus_tag="LR110_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4823698.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1647..2972
                     /locus_tag="LR110_002780"
     CDS             1647..2972
                     /locus_tag="LR110_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL4823699.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            2965..3315
                     /locus_tag="LR110_002781"
     CDS             2965..3315
                     /locus_tag="LR110_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823700.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            3312..3491
                     /locus_tag="LR110_002782"
     CDS             3312..3491
                     /locus_tag="LR110_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823701.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
BASE COUNT         1236 a          520 c          703 g         1054 t
ORIGIN      
        1 cattggtaac ccaactaacg acttaactgt tactgaaaat tatttaatag tttctccatt
       61 acttgctcga gcagcttcta aaacgatacg agtgacagga acgatgattt ttgataaagt
      121 tggttggcct tctagacctc ctcaatcacg gaattggaaa gaaaaaagaa atggttattg
      181 gtactatgga aaattgtatt tacaaagtta taaatcagct ggaaacaatt ggattgcgac
      241 ttattcagga accttaacta gaaagtaggg attttttatg ccagaatttg tgaacacacc
      301 aaatttttat caagatagcg atattctaag taaaattaat ttaccgaaag aagaatcagc
      361 tttacaagtt cagcgaactt tacaggagac acaaaaaatt caaaaatcaa tttctaatct
      421 agaagattct gaagaaaaaa gaaaaaaaag agaccaaaaa acatatcaaa aagaaaaata
      481 atgattttaa aaaaagagtg tctagtcgac attctttttt tctaattgaa agtgttcata
      541 aaatcaccca tttctggaca cttccttttt ttgctttatt tttcaaaaaa acaattcatt
      601 tcatgttcat aaacccataa caatatctat gttcagttaa actatatgga taaagttatg
      661 ttacaataaa attaatgaag gagtgatttt tttgaaaata ggatacgccc gggtatctac
      721 tggtttacaa aatttggatt tacaagaaga cagcctaaat aaatttggtt gtgaaaaaat
      781 ttttactgat catatgagtg gggcaaaaag taatcgacca ggattagaaa tggccattga
      841 ttttgttcgc tctggcgata cacttgtcgt ttggcgctta gatcgtttag gtagaaatat
      901 ggaagatttg atttcaatcg tcaatcgact gaatgagcgt ggtgtgagtt ttcatagtat
      961 tcaagaaaat attacgatgg acaaatcaag ttctacagga cagctcatgt ttcatttgtt
     1021 tgcggcattt gcagaatttg agcgtaattt gatcctggaa cgatccgcag caggacgagc
     1081 agctgctcgt gcaagaggac ggtttggtgg tcgtcctgaa aaattgtctg aacaagattt
     1141 ggaattactg aagactttag tggatagcgg tacacctatt aagactattg cggaacggtg
     1201 gaacgtctca agaaccacca tttatcgtta tttggcaaaa atggaggaga aaaatggaaa
     1261 gacaaattct cattagtttt tgtcatttaa tgctgaaaat ttttgccatt tatctagaaa
     1321 agtgacaata gtatgagaat tttttgtaag tgtctactga taacttatta tcaaatgttg
     1381 taaggatttt attcatttgt taatattaaa gaacggaact ccaaaaaagt ccgtactcta
     1441 atttgcagtc tctcactctt ttagagtgag agtttttatt agattttcaa attaattagt
     1501 aaagtgatag aataaattga tcatacaaat cataagaaga agcagggtga tagcatacct
     1561 atgcttcttt ttttatgtat ttagctattt cttctttaca acatcgaaca tatgtttgta
     1621 taatgtggaa gaatggagtg ttatttatga tggattattc aaaagagcca gtaaatgatt
     1681 actttttaat cgatatgaaa agcttttacg caagtgtcga atgtatagaa agaaatttag
     1741 atccattaac aacagaactt gttgttatga gtcgatctga taatactggt tcaggattga
     1801 tattagcttc ttctcctgaa gcaaaaaagc ggtatggtat tacaaatgtg agtagaccac
     1861 gtgacttacc acaaccattt cctaaaacac tacacgttgt tccaccacgt atgaacctat
     1921 atatcaagcg aaatatgcag gtaaataata ttttcagaag atatgtggct gatgaagatc
     1981 tactgattta ctcgatcgat gaatcgattt taaaagtgac aaaatcactg aatcttttta
     2041 cgactgaaga aacacgaagt cagcgaagga agaagctcgc tcaaatgatc caagaacgta
     2101 ttaaagaaga gttaggtttg attgctacgg taggcgtcgg cgataatccc cttttggcta
     2161 aattagcctt agacaatgaa gcaaagcata atgaaggatt tattgcggaa tggacgtacg
     2221 aaaacgttcc tgagaaagta tggaatattc ccgaaatgac ggacttttgg ggaattggtt
     2281 ctcgaatgaa aaaaaggctc aaccaaatgg gaattttgag cattagagac cttgctaact
     2341 ggaatcctta tacgattaag aatagattag gtgtgattgg attacagctg tacttccatg
     2401 ctaacggaat cgatcgaaca gatatagcaa tcccgccaga accgacaaag gaaaaatctt
     2461 atggtaattc acaagtgtta ccacgagatt atactagaag aaatgaaatc gagctggtgg
     2521 taaaagaaat ggcggaacaa gtagccattc gtatcaggca gcataattgt aaaacaggat
     2581 gtgttcattt aaacattggt acgtcgatct tagaaacgag accaggattt tcacaccaga
     2641 tgaaaatacc tatcacagat aatacaaagg aattgcagaa ttattgtctt ttcttgtttg
     2701 ataagtatta tgaaggacaa gaagtaaggc acgttggaat aacctatagc aagcttgtgt
     2761 acaccgattc gcttcagctg gatttattta gtgatccaca aaagcaaatt aacgaagaaa
     2821 atttagataa aattattgac aagattcggc aaaaatatgg ttttacgtcg attgtacatg
     2881 cttcttcaat gttagaaagc gctagaagca ttactagaag cacacttgtt ggtggtcatg
     2941 cgggcggaaa tgggggaatt aaaaatgatt gattacaatg atcgtggacg tttaaagtgg
     3001 gcagggtttt atttatctga tcatactgaa gaaattgatg ccgacaatga acaacgaacc
     3061 agtcaaaatt tagcaaaaga acaaatgacg actgaagaga ttgcggaagt attgaacgct
     3121 gcagtcataa aaaatagatc agtatctatt caaaaagaag aacgaaacgc tgaaggatac
     3181 tatccagaag atattgttgg caagatctta ggtagtgatg agctggggat ctacgttgga
     3241 aaagacaaaa ttcactacga cgaaatacgt catatcgaat tttaccagga taataaatgg
     3301 agtcgattag aatgaacatt atccagcaat atgagctaaa gtatataacc tttgatcagc
     3361 tatcagaaga gatttggggt tatggccaac gtttgataaa tgaggttggt gttgagcgct
     3421 tctctttcta tgtagaagct gcagcaggtt atcataattt taggttttat attttcccgt
     3481 tatatatata aggtatttta cggattctat aga
//
LOCUS       DAFFSV010000182         3414 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFFSV010000182 DAFFSV010000000
VERSION     DAFFSV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.185"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(159..1046)
                     /locus_tag="LR110_002783"
     CDS             complement(159..1046)
                     /locus_tag="LR110_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL4823702.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1043..1912)
                     /locus_tag="LR110_002784"
     CDS             complement(1043..1912)
                     /locus_tag="LR110_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL4823703.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2049..3335)
                     /locus_tag="LR110_002785"
     CDS             complement(2049..3335)
                     /locus_tag="LR110_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL4823704.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1029 a          718 c          593 g         1074 t
ORIGIN      
        1 ctcctttact ctttaagttt accttaaaat gaaagagttt tcgagcacaa acttaaaacg
       61 ttatcattta atttttctca acataaaata acacacgtcc caaaagtatc tttttacttg
      121 gaaaaatgac tttgaaggaa cgcgcttatt gtgttttttt attttacaga gccgatcatt
      181 ccttgaacga attgcttttg caaaacaaaa aatacgatgg cagttggtaa agtagcgatg
      241 actaatccag ccatgatcat tccatagtct ggtgtatagg aagcacccat agcagataaa
      301 acaagcggaa ccgtccgttt atcaggagat tgcaaagcta ctaagggcca taaataattg
      361 ttccagctac tcatgaatgt gatgattgca gccgcggcat aggtattttt agccgtcggc
      421 atatacactc taaaaaaaat acttagttct tttagaccgt ctaatcgagc ggcttctact
      481 agatcttttg gaaaagattt aacattttgt cgaaagaaga agatcaaaaa agcagtgcta
      541 acagatggca ggatgacaac caagaaactg ttgattccca aaaccgttcc gttcaattgt
      601 gaaaacattc gatataaagg gatcatcaat gccgcaaaag gaaccatcat cgatagcagt
      661 agaatattga aaattaggtc ttttcgttta ctttgataaa tctcaaatcc atatcctgcc
      721 attgacgaaa tcagcaatgc aaacacagtg gtcaccaaag cgatcatcgc tgaattccaa
      781 agggaacgag taaaattcag ctcattggaa aacaaatttt gaaaattgat gatcaagtta
      841 ctgccaatct ttatttttcc cgatgtgata tccacgggtg tattcgtcat tcccaaaacc
      901 atccagacaa aagggaaaat ggaaataaat gataggatgc ttaatgctgt atactttaat
      961 actgacccaa ttgtcgaacg aagttctaat gcttgttttt tgacgttggg gcgttcgatt
     1021 gtttcttttt ctaccatatt tttcatcgac tttctcctcc tactttcgtt tggattatcg
     1081 acaatatcac aataaaggaa acaataacaa aggaaactgc tgccgcatat ccaaaattag
     1141 gcgtgaattt ataactcaaa ttatagatgt attgagatag ggtggtcgtt gcatttgctg
     1201 gtcccccgcc agtaatattt tgtacttcat caaaaagctg caatgtcccg atcgtcgaag
     1261 taatggaagt aaacaaaatg attggtttta aatttgggat cgtcactttg ataaactgct
     1321 gccatttaga agcaccgtca atagatgctg cttcataaat ctccgaatca atattttgca
     1381 tgcctgaaag gaaaaaaatc atattgtatc cagtccaacg ccaagtgatc gaaagaatga
     1441 tcagaactct cgcccaaatt ggatgagtca accagccaat cggtgtgtca atcataccaa
     1501 tgcccaacaa caagttgttt acaaggccag attgagaaaa tagacttttc atgatcagag
     1561 aataactgac catcgaagtg atacatggaa gaaagatagc ggttcgaaac aaccctttat
     1621 atttcaactt tttatcattt aaaaggttgg aaataaaaag tgctagaaac aacatgatcg
     1681 gtacttgtac aagtaaatac aggaatgtat taaaaaaagc ttttttaaaa gttgcatctg
     1741 tcaacatccg ctgataatta cctaacccgt taaatgtcat attgacaggg ggaccacttt
     1801 gaaaagacgt gatcaaagcc aaaaccatcg gaataaaaac catgattgat atcaaaatga
     1861 ctggcaagat taaaaaagca tgcccgtttt tgaataaatc tttctgcttc attcttttcc
     1921 ctccaattct atcattgatt atctacatga aaagacaaga aagacaatat ctgtaaggca
     1981 gacgtgatct tttttgtctt ttcgtttgtc tgatgaaaaa gcgaagaaat tcactttttt
     2041 accaccgatc aattagccaa ttgtgcttct acttgttttt gtgtatctgc tagtaccttg
     2101 tctgtctctt ctccattgat gattcgatga agagcttcag ctacgactgc ttcaattgca
     2161 taggtctcat gtccgtaatt cacttctggt atttcagatg tccatgttga aaaatcgtca
     2221 aagatctttt gaccactgta aaattcagaa gcttcttgat aagcttcttg ttcattcgcg
     2281 ctaagcatcg tagatactag tccgatttct ttcgcaagag tgcccatcag ttcttcattc
     2341 gaagcaaacg ttgcttctag gaaatctttc gctgaatctt ctccagctac tcctttgatc
     2401 acataccagc cagcgccgcc aaggttagaa gcttgcgctt tttgcagatt ttctggaagt
     2461 gcaggtattg gtgcaatctt ccattttcca gattggtttt ctgctccttg gatcgttgaa
     2521 gaataccaag cgccagttgg gctagaagct actgctcctg attgtatcgc agttactcca
     2581 gcattccagt cagaggtttg ttcgactaga tcttctttca aaagagtggc aaacaattca
     2641 agaccatact ttaaagatgc attgtcttgg atcgtgactg tctccccatc ttcagatgta
     2701 taccattctc ctgcctgttg cataatcatc cttactcgac caaggtcaga aggattgact
     2761 tcagcgattt tctttcccgt cttttctttg acatctcgcg ctacttgaat atactcgtcc
     2821 cacttcagat tctccatatc ctcttctgta taacctgctt ctgccatcag atccgtacga
     2881 tagaagtttg ctgttacgcc cgaatcgaaa ggaacaccgt atattttatc tcctactttg
     2941 tttaccgcaa atttatacgg tgcaaaatga tcttccttta caatagcaga caaattagcg
     3001 aatgcatctg gataagaagt caaatagcct tgtatcctat aatcttcaat caagacgata
     3061 ttgggcaatc cttctgtatt cccgctcgct aaagcggtgt tcagtttctg aacgatatca
     3121 tcctgagaca tcgtaacaac ttctatctct gtttcttcgt tctcataaac tttttttgct
     3181 tcattcactg ctttgatatt gaatgtctca tcccacgccc agacagtgac tttttcttct
     3241 ttgtctgctg tactactagc acttccgcat cctgttaaga aacctgatgc tgccgctaaa
     3301 caaacgactc cagcaattaa ctttctgatg ttcattttta tccgctcctt ttccatccat
     3361 tagttgttta cgtttgctac attcatcata ccaaaatttg aaaacggttt cttt
//
LOCUS       DAFFSV010000183         3363 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFFSV010000183 DAFFSV010000000
VERSION     DAFFSV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.186"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..799
                     /locus_tag="LR110_002786"
     CDS             <1..799
                     /locus_tag="LR110_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL4823705.1"
                     /translation="AIQKWIELKFSIIQTWIDLKYNAITTWNNIKQFFKDTWKNIKDT
                     AYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNS
                     LREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRD
                     MVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASET
                     KEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            789..1529
                     /locus_tag="LR110_002787"
     CDS             789..1529
                     /locus_tag="LR110_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4823706.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1526..>3363
                     /locus_tag="LR110_002788"
     CDS             1526..>3363
                     /locus_tag="LR110_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL4823707.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1262 a          414 c          647 g         1040 t
ORIGIN      
        1 tgcaattcag aaatggattg aattgaagtt ttctattatt caaacttgga tcgatttaaa
       61 atataatgcc attactactt ggaataatat taaacagttt ttcaaagata cttggaaaaa
      121 tattaaagat acggcataca atacgtggat ttctataaaa aataccatga ttaatacatg
      181 gaataacatt aaggactctt tctggaatat tgttactgga attgttaatt ctgctgaaaa
      241 tgcatggaca aatctaaaaa atggtgtttc aaatgctatt aatcgggtaa aagaaatctt
      301 taattcgtta agggaagtca atttatttga aattggcaag aacatcattg atggacttat
      361 aaatggtgta aaagaaaagt ggaatgcttt gaaagatact atcaaaggta ttgcaggtaa
      421 tatcaaaaat tggattaaag gagctttaga tattcattct ccatcaagat ggatgcgtga
      481 tatggttggt aaaaatattg ttcaaggtat tatcgttggt attgataaag agcaaagtaa
      541 gttagatcaa accatgacaa atttagtaaa aacaccgtct gttcaacctg tagtaaatgg
      601 aataagtcac ctaccaatta cacaaacaaa gcaaaatata tctgctagtg aaacaaaaga
      661 aattcattta catttaaatg tttatggaga tttacctgat tcaatggttc ggaagattac
      721 cacaaaaatt aaaacagaat tgacaagaca aatgaaacga gatgctggtg cagtaggagg
      781 aacattatat gcaacttaag agaggacaat tttttattaa tcaacattat tcttctgaat
      841 ttaatgtgta tattcaaaac aggcctgctt ctgtttcagc tagtcgagta attgaattga
      901 gagagcgaga aggaaatgat tctatcatta ttgataaagg ctattacaga aatgtgacta
      961 gaaagattga gtgttactat aaagcacctt caattgatgt ggtgcaggag tgggaagatc
     1021 gaatcactga atggttagat atgtcgtctt atagtgattt tattctttac tatgatgaac
     1081 aatatattta tcaagctgta gtaatagaag gacctgaatt taaaggaaca agaaaaacag
     1141 gaaatattgt cccttttgaa tttacagtga gcattcgacc atttaaggaa aattacaatg
     1201 gtagatttac tattcgacaa acagaaactt ttgaaattta taacccagag aagtattctt
     1261 caaaaccgct tattaaattg agtggttctg gagatgcttc tttttatatt aataaggaca
     1321 aatatgattt gaaatcgttg gacagagaat tatttataga ttctaaatta gaagaggctt
     1381 accgaaaatt agatggtaat ttagaacatc aggatcaagt cactttattt ttagattttc
     1441 catttttata tcctggaaaa aatgaaatta aatggacgaa aaatattcat tcatttgaaa
     1501 taatgcctag gtggtggaga aaagtatgaa accaagaata tatagtccta ctgaaacaga
     1561 tttctcaacg aatggcttag gaattttaaa ggattgtaca agatgtgaaa tatatgaagt
     1621 agctaatgga aaatatgaat tagagttgga ttatccttta ggaactagat ttgatgaata
     1681 ttttgaaaat gactatcaaa taaaagcaaa gccaaacgat caagaagagt atcatatctt
     1741 ttttattgat gataaagata tagatacttt tttaaataca gtaactattt atgctcagag
     1801 tcgtacaaat cgacttggaa gacgggtagt cactcttgcg gaagtagact ctaaaactgg
     1861 tcaagaagcg atatcaatta ttgaaaccaa aatggataaa aaatctgaca tacgacttta
     1921 ttctgatatt acggccgttt ctagcacaat ctttgaagcg agaaatgttt taaattgtat
     1981 tgctggtgaa caaggatcat tgcttcaata ttggggtgga gaaattaaac gtgaaccatt
     2041 taagctttct ttgttaaagc gaagaggtcg caataatgtt ggaacgattc ggtatggaaa
     2101 agatatgtct ggtttaaagg tcaaattaga ttggacaggt ataaaaacaa gacttattcc
     2161 atatgctgat cctcaaagtg aggtaggtac gactaatcga atttatggtt cgccagtaga
     2221 tagtgcctat attaataact atcctgatgt gtatacagag catgttcagt ttacagaaga
     2281 acaaggagta aaagacatta agagcttaaa taaaatagct aaaaattact ttaaaacgat
     2341 caatcctggc tgtgataagc ctaaagtttc tattacggtt gaatttgata agttgactga
     2401 tagtgaagaa gcgaaagaat ttgcgaagat tagaaattat ggtctgtttg atacatttaa
     2461 aatatatcat aaaaaatata atatttatct tgaatcaaag gttagtggac tacaatacga
     2521 ttctttatct gaaaaaactt tgaaattaga agcaggagac attcaagttg ctttttatca
     2581 gcaacaagct gtaactattc aggataaatt aaaagattat gcaacgaata attatatgag
     2641 tgattttaat gattatgttt cttcaatgat tacaggtcaa ggcagtgcag gaggatatgt
     2701 aagtttatgg ccaaaagaaa aaccttccaa catctttatt atggataatc cagatttaga
     2761 aaaggcaaga gaagtactaa gaatgaataa aaatgggatt gctttttcta agaaaggatg
     2821 gcgggggcct tttacttcag cttggacatt agatagtata tttaatgcca attttataca
     2881 aactggatta atcaaagcag atatctttca aaattctttt aacaagactg gagatgtatt
     2941 gaaattagtt aatggactac tccaaatttg gaacaacaaa aagaaaataa tggagttaac
     3001 taaaaaagga atggaatttt ggaattctaa gggttcaatt ggaacgattg gaacaactga
     3061 ttctgctggt aatccttttc ctggggcttc tactccaacc cctattgaag ataattcttt
     3121 agttattcgt acaaatggag acggcaaata tattttgatt tctcctaaag taggaaaagg
     3181 attagtttta ttaggaaatg gtaaagcaat ttattttgga gacttagatg tacaaggaaa
     3241 acttacagtt aaaggaaaag aaataattcc tggacaaggt ggtggtccat cgggaggtgg
     3301 agaatctact ggtgggtatc ctagtgaatt aaaaacagat gcagaaaaga gagcttggag
     3361 aat
//
LOCUS       DAFFSV010000184         3330 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFFSV010000184 DAFFSV010000000
VERSION     DAFFSV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.187"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(103..258)
                     /locus_tag="LR110_002789"
     CDS             complement(103..258)
                     /locus_tag="LR110_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823708.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(717..974)
                     /locus_tag="LR110_002790"
     CDS             complement(717..974)
                     /locus_tag="LR110_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL4823709.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(967..1236)
                     /locus_tag="LR110_002791"
     CDS             complement(967..1236)
                     /locus_tag="LR110_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL4823710.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(1367..1546)
                     /locus_tag="LR110_002792"
     CDS             complement(1367..1546)
                     /locus_tag="LR110_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823711.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(1927..2634)
                     /locus_tag="LR110_002793"
     CDS             complement(1927..2634)
                     /locus_tag="LR110_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823712.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(2627..3064)
                     /locus_tag="LR110_002794"
     CDS             complement(2627..3064)
                     /locus_tag="LR110_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823713.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(3079..3330)
                     /locus_tag="LR110_002795"
     CDS             complement(3079..3330)
                     /locus_tag="LR110_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823714.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
BASE COUNT          962 a          656 c          453 g         1259 t
ORIGIN      
        1 tcgaagtata atttgattcg tcagcaagca tcgctacatc ccaaacactg atcataaaat
       61 cttttaaacg ttcaatcatt tcagcaatat tgctaaaatc agctaatttt tctttagaat
      121 aaaaaaaaca tttatatcag aatcaataaa ttctattttt tttattacta tatcgtcatc
      181 attatcttga ccatttatgt ttattgttcc ctgagtgggg agtacttctt tcaaattcaa
      241 gttaaattta tattccatta cttctcctcc aatatataaa aattacttaa ctgtatccaa
      301 atttaatatg taaataaatc attaattttt agctaaattc tatgagaagt ttatttttgt
      361 tgtttttgct aattaactaa aactagtaca aaaaatttaa aatagattat taaacctatt
      421 gcttattcta ttcagtcata tatataagcg tcatccttta gtaactaaca tcccacacta
      481 caatagtatt ataacatatc tattcaactt caaattgttg aaatttaagc catttatatt
      541 tacttttcct caactttaga aatgatttta ctaaccttat atccgtaata cgcgtaacaa
      601 acacattcat taataacaat caacactaag ctatattagc tttttaaatg actagggtaa
      661 ataaaataag gatcaaaaac ttttaagttt ctgacccttt ccttacttcc gattggttaa
      721 tagtgatctt ttgcagaata aataaatatc gtttcatttt caactctata tatcagtcta
      781 tgttcatctg taattcttct ggaccatttt ccagataaat catgctttaa tggctcaggt
      841 tttcctaatc cagcaaaggg ggaacgatcg atatctttta ttaacttgtt aatctttttt
      901 atattgcttt tgtttccttg ctcatgccaa taaagataat catcccaagc atcatcagac
      961 caagccttaa tcatcagatt caacctcgat taagtcatgt gttttaaatg cacctttgga
     1021 gaattgttca tctcctcgac gaattttttc catgacgtaa ttattagaaa gtgttctcaa
     1081 cgtttcttgc atagaatcat aatctctttt tgataataca acaactgtat cttctacatc
     1141 tttacttgtt acaataagtg tttcagcatc ctcattaact tgtttcatat aactacgtaa
     1201 attttggcgg aaatttgaat aagctactgc ttccattcct ttcacccctt atttatttct
     1261 ataaaacaat tgtacattat attgtacaat taagcaaatg attaatattc taacttctta
     1321 taattaaaag tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg
     1381 ctggtacctc aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa
     1441 tcctggattc acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt
     1501 catccaacgt cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct
     1561 ttcatcgaaa taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa
     1621 gggttaacac aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga
     1681 agtttaaatg tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca
     1741 atagaggaat gattttattc atatggatct ccttctttct cttaggttat cgggctttgc
     1801 ccgcttattt cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga
     1861 accattccct taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat
     1921 aaatggttat tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag
     1981 ttcattttgg atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt
     2041 cgtgtaccag tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc
     2101 gggtacagtg agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa
     2161 aaatctagta aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc
     2221 catttcttct tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta
     2281 atagtgtttc ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag
     2341 gtaaacattc atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa
     2401 tgtgtcagga cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg
     2461 actcgtttca ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc
     2521 ttccatacaa ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt
     2581 tgttaaaaat tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt
     2641 tctcctccga ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta
     2701 gccagtcgct agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt
     2761 ctaaggcttg atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata
     2821 gtaaggcttg gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt
     2881 acgtcgcaat gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca
     2941 atttttcctt gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct
     3001 ccattttttg tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag
     3061 ccatattttt tctccttttt agttctctaa tttggttaac caatattcga tttcataagt
     3121 ttccatgttt tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag
     3181 catagtcggt gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa
     3241 agcttctgct actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt
     3301 tacagtcaat aatttgtgtt gtgttttcat
//
LOCUS       DAFFSV010000185         3274 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFFSV010000185 DAFFSV010000000
VERSION     DAFFSV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.188"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..2177)
                     /locus_tag="LR110_002796"
     CDS             complement(<1..2177)
                     /locus_tag="LR110_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823715.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTSNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="LR110_002797"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="LR110_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          902 a          729 c          528 g         1115 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt gcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt ttactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcat
//
LOCUS       DAFFSV010000186         3220 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFFSV010000186 DAFFSV010000000
VERSION     DAFFSV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.189"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..298
                     /locus_tag="LR110_002798"
     CDS             <1..298
                     /locus_tag="LR110_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL4823716.1"
                     /translation="KQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNLDSVT
                     AGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
     gene            298..1674
                     /locus_tag="LR110_002799"
     CDS             298..1674
                     /locus_tag="LR110_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL4823717.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(1960..>3220)
                     /locus_tag="LR110_002800"
     CDS             complement(1960..>3220)
                     /locus_tag="LR110_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HBL4823718.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGEREK
                     QFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEINCVVADAGYKTP
                     KFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTRER
                     YREYKSNPKECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKR
                     KQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFC
                     SKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTK
                     ARFKRLIAITVSSIVMFLWLFRMGVIP"
BASE COUNT         1121 a          492 c          587 g         1020 t
ORIGIN      
        1 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
       61 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
      121 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
      181 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
      241 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
      301 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
      361 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
      421 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
      481 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
      541 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
      601 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
      661 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
      721 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
      781 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
      841 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
      901 gtagaaatta aaggtctatt cgatgggcat aatagcggtg tagtaagtgc agcacaagaa
      961 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     1021 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     1081 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     1141 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     1201 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     1261 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     1321 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     1381 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     1441 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     1501 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     1561 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     1621 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     1681 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     1741 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     1801 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     1861 acttatcaac ccgaatctgt agaggatatg caagatgttt taaaagatgt gtttggtccc
     1921 ttatttgaaa aaatgcttca aggagaattg aataaccatt tagggtatga cgcccattct
     1981 aaagagccaa agaaacatga caatcgacga aacggttatg gcaataagac gcttaaaacg
     2041 agctttggta aagtaaacat tgatgttccc agagaccggg aggcttcttt tgaacctgag
     2101 ttgattccta atagaaaacg agatgtctcc gacatcgcag actgtagaca aaagctgttc
     2161 caaactcgta actcgagctt ggaacagctt tttatctact tcgtttcgaa tttttgaaca
     2221 aaaaaataaa ggctccttgc ctttccatag ccaggtggct aatttcttta aattcattgc
     2281 agcaaacact agcatcgtat gcgtcgcgac tttttccaag cctcgatact tggtccaacg
     2341 catcccatgc ttttcttttg catccgcaaa tattcgttca atcgtttgtt tccgtttttt
     2401 gtaaattgat ttattcagat ctgttaggcg aagatgttcc acttcatcca tcagatctcc
     2461 ccaaacatgt ctcgtgatga ctcgttgatg atttttactt ttggtacatt ggtttagcag
     2521 aggacagttc acacattctt ttggattgga tttgtattct cggtaccttt ctcgagtaac
     2581 cgttgaaaaa gcgagcattt gatcttgcgg acaaatataa caatcaaaat actcgtcata
     2641 gagaaactca ttttttgata atagcccctt tttccctttg ggccttgaat aaggcaaaca
     2701 aggtcgaagg ttcaaatgag tcaaaaagtg aacaaactta ggtgtcttat acccagcatc
     2761 agcgacaaca caattgattt caggaaaaag tctcttcgat tgtttgacga gctgaaaggc
     2821 gacttgactg tcatgaatat ttccaggagt gatgattgtg ctaagaataa atccattgtc
     2881 gtcgcatgaa gtatgggcag aataagcaaa ttgtttttct cgttcgcctt tgacgtagta
     2941 gccgctttcc ggatcagctt tacttatttt tctttctttc agcttcactt ccttttctaa
     3001 gtccaaagta aaagggcgtt ttccagcctc tattcgttga cgattgatct catcttctaa
     3061 ttcatcctga taggctttcg cttcatcatg ggtcatttct ttcgtaaact tatgtttatt
     3121 ggcgttagct ttgatgtggg tagaatccag atacaaatta tcctctgtta caaaacctgc
     3181 tttaactgct tgttctaaaa tataggcaaa aatatcctca
//
LOCUS       DAFFSV010000187         3208 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFFSV010000187 DAFFSV010000000
VERSION     DAFFSV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.190"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            377..1816
                     /locus_tag="LR110_002801"
                     /pseudo
     CDS             377..1816
                     /locus_tag="LR110_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2161..2328
                     /locus_tag="LR110_002802"
     CDS             2161..2328
                     /locus_tag="LR110_002802"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4823719.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            2587..2802
                     /locus_tag="LR110_002803"
     CDS             2587..2802
                     /locus_tag="LR110_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823720.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3020..3208)
                     /locus_tag="LR110_002804"
                     /pseudo
     CDS             complement(3020..3208)
                     /locus_tag="LR110_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          948 a          548 c          678 g         1034 t
ORIGIN      
        1 aatcctatgg aattgaatat catagcaatg tatttgaaaa gcaaattgga aaacaaatag
       61 gtgcaacacc ataaatatga aaaaattggg aaaaagtaga atggaaattt tcttacgtct
      121 cacgagatag ctcacactat taatctgaag aacaatgaag tcgactttta gaaattgcta
      181 gaagaaagta aagcgaatta tcactagtta ctatagagta agttcaaata aagcatctca
      241 aacttacatg gttacatgag aataacaaat cgaaattact caagcttcct atggctagag
      301 tcgcgagaag ttgttgggca gtagacatag gttatattgt tcttagctac acgatcatta
      361 gaaaggaaat cctattatgc atagttatca agaagatgca aaagtccaaa aaaatcgctg
      421 gtggattttg attgctatat ctatgtttac cttcatgtcc acattggatg gcagtattgt
      481 caatattgca ctacctacca tctcagatga tatgggcata ccaatgaatc aagcggaatg
      541 ggtagtgtcc gtttatctga tgattgtctg ttcttgtttg ttgctgtttg gtaaaattgg
      601 cgatagctgt gggaaaatca aagtatttcg gattggtacg ctgatctttg tcttgggctc
      661 gctgttgtgt gggttcaatc attcgctagg tatttaattg ttcgcacggg tggtacaagc
      721 cattggtgct agtatgacta tggctacaaa tgccggaatc atcacggaag tctttcccat
      781 acatgaacgt ggtcgggcat tgggagcaat cggtgcgctt gtttcattag gctcgattgc
      841 tggaccaggt cttggcggct tgattttgtc ccaattttct tgggcgtaca ttttttggat
      901 caatgtaccg gtgggaatcg taacgatgtt gattgctgaa aaattcttac caaaagatat
      961 caccatgagc ggccatacaa tcgatggagg gggctttgct gccattgcgg ctacgattat
     1021 cagccttttt ggaacaatct ttttggggca agaaagtggc tttttccatc cagtccccct
     1081 tgtactgttc gtaatttcag tgattgcctt tgtcagcttt ttaaaagttg aaaagaaagc
     1141 agcctctcca ttgatttctt tcggtatttt taagaacaag atttttacca tgagcttgat
     1201 tacagccttg ttgatttttt cttccaactt ctttgttaat gtagtgattc ctttttatct
     1261 gcaaaatgct cgcgggttga gtcctagcta tgccggactg ttgatgatgg tctttccctt
     1321 gatgatggtc gtcggggcgc ctttaagcgg atacctgacc gaccgggttg gcccggagat
     1381 ttttgtatta gtaggcttgg gtttattgtc ggtaacccaa gtcatgtata tgtttttgtc
     1441 agtggactct cctttgtggc tgtacattgt cgctactgcc atcatgggtg ccggcaatgc
     1501 cttgttccag tccccaaata atacgatggt tatgagttct gtcactatcg aaaatttggg
     1561 actggcaggc tccatgaatt cctttgcccg aaatttgggg atggtcatcg ggatttcgct
     1621 ttctacgacg gtcttatatc aagcgatgtc tgctaagatg ggccaacgag taactaacta
     1681 tatcgcagat cggccggatg tctttttata tggaatgaag attacctttt taggcagctt
     1741 tgttttgtgt tttgttgcct ttttcctgac tatctggcgt atcaaaaaac aaggaggtat
     1801 tcagaaaaat gtctgaaaaa ccattgttga agaatttttg gaggcctgcg tatgggtgag
     1861 tgggatttcc atggatgatg gaagatgaag tgaacttctg aaaaccgaaa aactcaagta
     1921 ttatcttcaa aatactagta ctcaattaga tgctcgattt gattacaaga agactgcgaa
     1981 agccactggc aaccgcaagc taggtatcca ccgatggact acgttttaga ggcaactggt
     2041 tatgaatacg gagctaacgc tctcatctgc ataagaatgc aacacccaga ttatgtaaag
     2101 attttcgata cttctgtcaa aaaaatcatc aagatagata aatgatttcc gtctagtcaa
     2161 attggttcag aatgtggaca cacagatggt aagaagtcat tcacaattcg agaatggact
     2221 tgtcctattt gccgtacaca tcatgatcga aacgtcaacg ttagtatcaa tattttgatc
     2281 aagggctatg tatacaagca ttgccttaga aaacatcaag aaccttagga acaacgggga
     2341 tagattggtt aataagagat atcgctgtta gtaaagacat aggcttgcaa gtatactctt
     2401 ttatcaagaa ttccgtggct ttagtcatga taggtttaat actatgaaaa ttttctttag
     2461 catttttgat ttaggttcct tttctaatta taataaaatg accactacag taacctactg
     2521 aaatatataa acagccactg tagtaccatt ttcaaagtag tctatgagag gagaatcttc
     2581 attcttatga acgctggaat tgcggatatg aatttaataa aaaaaaccct taatgatttt
     2641 acttccaatt caatatcaaa aggtactggt atcaatttaa gcacaataaa aaagctgaaa
     2701 agtggtgaaa gatcagtgga aaagctgaat cttttagatg ctataaaaat aacggaattt
     2761 gctatgaaaa atggaaaagc tgagattgaa atatggagat aattatcgtc tagtgaacta
     2821 cttgccactt agctgaattt aacgatcctt agtgcttgaa gtgggagatt cttgggaata
     2881 gagcatactt gcaagcttat gtctttatta acagcgatat ctcttattaa ccaagctatc
     2941 cccgtagttc taaggttttt acttttatct aagctaatga tcgtattcaa aggaaatttt
     3001 tgacccttag aaagtctcat tttatcagac atgagcttag gcattgctga attttgttaa
     3061 ttcggtttta ggttgtgctt tgagcataat atgaaagtag tctttatcac gatttcactc
     3121 aactaaggta atgtgatgtg attctgatat tctttcaaag atagtttttt gcataagcta
     3181 acattacatc atcaatgaga tgtttaca
//
LOCUS       DAFFSV010000188         3183 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFFSV010000188 DAFFSV010000000
VERSION     DAFFSV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.191"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(403..663)
                     /locus_tag="LR110_002805"
     CDS             complement(403..663)
                     /locus_tag="LR110_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823721.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            738..941
                     /locus_tag="LR110_002806"
     CDS             738..941
                     /locus_tag="LR110_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBL4823722.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(1082..1567)
                     /gene="ssb"
                     /locus_tag="LR110_002807"
     CDS             complement(1082..1567)
                     /gene="ssb"
                     /locus_tag="LR110_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL4823723.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1570..2469)
                     /locus_tag="LR110_002808"
     CDS             complement(1570..2469)
                     /locus_tag="LR110_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL4823724.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2482..3075)
                     /locus_tag="LR110_002809"
     CDS             complement(2482..3075)
                     /locus_tag="LR110_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL4823725.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
BASE COUNT          868 a          617 c          543 g         1155 t
ORIGIN      
        1 ttcctttcgt tagttctttc tttattgatt tgttggccag aatgaatcaa cgaaaagcga
       61 caaggacaag ggcgcgcagc gtgccattta cccttgtcct tgtcgctttt tggtgataaa
      121 aggcaacttg ttgccggtct ggcaaatcaa taaagagata gcgaacagga acgaaccttt
      181 tggtgagagg aagttttttc tattttgtct tgctttgcaa gacaccatat cttagtgaac
      241 gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg gttggagtga gagaaaggtt
      301 tttcaattga gaaggcactt gtgtcttcct aaaaaggtct tttcctgttt ctatgtaaaa
      361 caaaaagagc tgcaatcctc atttaggact gcagctcttc ttttatatgc ccatcgatcg
      421 cgtaaaatga tcggggtttc taatttgcat cgattcaatg actaattgat tccgtttatt
      481 gaaatgacct gtgacggcaa tattgtattt tccatcatcc ataattaaca atttatcgat
      541 aatttcaata tctgcaacta cacaattaat cgattcatga attgtttgta aagtaaagcg
      601 aaccaataat ggttgtgttt ttattactcg gattttatca agcaaaccaa catatttgtt
      661 catcacgatc acctcgctaa aattatagca aaaacagcta aaattgagaa tttttattcg
      721 attttagata tgctataatg atcaagacag taaagtttgt gacggtggct atccgattga
      781 aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac agaaaggaga agccttttgc
      841 ctgttgaaac ggcgctagga ttaatgatta gtttcggttc tttgatcgca acaatcattt
      901 ttggaatctt agcagttgta aacaacaatc aaaaaaaata accgtcagct ttagccggtc
      961 gacgattatt tttcaaaaat aatattaagc tggtcaccgt cttaaacggt gctgttaggg
     1021 gagttgttag cgcaactccc cttttcgtat ccattataac aactttattg atcttttaca
     1081 atcagaatgg taaatcatca tcactaatat cgattgattg tccgccaaat ggattttgtg
     1141 aagcgttcaa tccattgttg ttaggcgtag ctacatcccg ttgttcaaag gtggtagggc
     1201 ttggactgct ggacgattga tcagcgtgcg cacggctttc tgtcgctgct ttactttcta
     1261 atagttggaa attatcggcc acaacttctg tcacatacac acgttgtcct tgttggttat
     1321 cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa actgcccttt ttagctaatg
     1381 tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt aataaaatca gcttctcgat
     1441 tgccgttttg attcgtgaaa tttcggttga cagctagggt aaaagtcgct actgcagtac
     1501 cactcgcggt ataacgtaaa tctggatctt tggttaagcg tcctactaaa gttacattat
     1561 tgatcatctt tatctctcca tttcttgatt catttgttgt gatcttttaa actgccgttt
     1621 tctttcttga ttttcaattt tgatcacact tttcattagg tgatcaattt cgttgactgt
     1681 atctgtaaat gaaatggaat tcgcaaacgt gttaactgag cgatattttt cccactgctt
     1741 ttccaaatag tcatttcctc tgatcttatc catttctctt tcataataaa aggacaacgg
     1801 ttttgtatcc ccacgttttg taaatgtatt tcgaacactt ttgtataact ctttcgtatc
     1861 gatattttct agtttcggaa tggtaaataa gtcgtagtaa tcttttgctc tcgaattatc
     1921 tgtgccaaag ctgaacgtag cacttagctt ttctgcaata atttgttctg ttggataagc
     1981 tggaatctcc acttttttat cttcaaacat taattggtga tagtgtgtat ctacttcagg
     2041 ataaatagaa tctcctgttg tcacgtccat tttaaacggt acacgtactt tctctaaatt
     2101 agccaccaat ttaatttgaa atccaggata aaaatcggct tctcttgttt ccttcattcc
     2161 ttgaatagag aaaacaatgc cttcttttgt gggttttcta gttaaatcat taaaaatgct
     2221 cttcaatttt tcttcagtta atttgctatt tctaaatgtc gtatcgatat caattgttgt
     2281 ccttctatcg attccatatt ttgatcctaa taaaaagccg cctttcaaaa tgaagtcatt
     2341 tttgtacttt gattcagaaa tttgttcaat tagacgatcc atcataaaat gccgttgcac
     2401 taactgtgca tttaatcctg tactctttgc taaatttctt gtttttgctg caaattgtgt
     2461 cggtgtcatc tttttcctcc atcaatataa gctttgcatg tggtatttca tttctttttc
     2521 tacatgtagt tttgtcatgt aatctcttaa tttacttgga tctctcaatg ggtcttccat
     2581 gtactctttt aatgcttcta atttcatttc atattcaaat tcatatctcg gattccaaat
     2641 atcacaaagt gtacgctctt tatcatacat agtaatagga tttccttgaa aggaagttcc
     2701 ctctactttt cctagttcat aaacttcttt cttcacgaaa tgaaattgta actgctgctc
     2761 ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa gttaagtgaa tgaatttcgg
     2821 catattcgta gaaagctggt aaatatctaa agtcgtttca tgagagaaga ttcccctgga
     2881 aaatcgttgc tgaagccaaa ataaatcatc tattccatga ccatcctcta cataataacc
     2941 acgatcaact ttgatcaatt ttccttgatt caccaatctt tgtacagtgg ttgctgcgat
     3001 tccaaatttc ctggcttcct tcatagataa atttccatcg cgaatagcca acaattcttt
     3061 cagtttatct tgcatttact tcccctccct tcgttacaaa tacactacat caaatatatc
     3121 atagtagttt atttgtaaca agattaacgt ctatcgatct aattcgattt ctttttgaat
     3181 gcg
//
LOCUS       DAFFSV010000189         3001 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFFSV010000189 DAFFSV010000000
VERSION     DAFFSV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.192"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..781)
                     /locus_tag="LR110_002810"
     CDS             complement(<1..781)
                     /locus_tag="LR110_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4823726.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSM"
     gene            complement(901..1548)
                     /locus_tag="LR110_002811"
     CDS             complement(901..1548)
                     /locus_tag="LR110_002811"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HBL4823727.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1632..>3001)
                     /gene="uxaC"
                     /locus_tag="LR110_002812"
     CDS             complement(1632..>3001)
                     /gene="uxaC"
                     /locus_tag="LR110_002812"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL4823728.1"
                     /translation="FAKHQPIIDYHCHLDPAYLLENKQYKNLTQVWLNDKGAGDHYKW
                     RLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPIFEWSHLELQRYFDIDLTISE
                     KNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDDPTDDLWYHTELSKQDNSFKV
                     LPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGLKQALSQRVDHFHQIGCRLAD
                     HGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQFVTGIQLHLMSEYTRYGWTMQ
                     MHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVALFDTAEKSGCLPKTILYSLN
                     PTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMRQQLTTMAQQSLLSNFTGMLT
                     DSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYLSQIVENICYRNAYNYFNFFE
                     IGSRK"
BASE COUNT          958 a          623 c          412 g         1008 t
ORIGIN      
        1 tcatactcat aattaataat tgagcaagga gtaagaaaat agccgcacca attatagaaa
       61 gtaatgccct atttttaact aaccctttta aagattttaa caatgaaact ttgtattgag
      121 attggtcaat tcttactcgt tcagtagtta gtttataaca tattaaataa caaataagtg
      181 cacatatcga aaatattccc gcaacaattg taaaagttga tccaccacga actatttgag
      241 cgcctgaatc atctcttgta taaataaata atggtgttaa tacaccaatg accaaacttg
      301 ctaaagtagc tccaacattt cggaatgtcg atagcattgt tctttcttct gtaatagaag
      361 taatagctga tgccattgat ccataaggaa tatttataga tgtgtaaaaa atactccccc
      421 ataatatata cgtaacatac atgtatacta ccttaacgct catagaagct gaaatcatag
      481 aagtttgata cattaaaaaa cttgctaaag cgactggacc actcattctt aaaatccacg
      541 gtttaaactt accctgctta gttgctttcg atctatcaac aatacgcccc atagttacat
      601 cagtaactgc atcgataaat cttgctacta aaaataatgt tccaacggca gctccgctta
      661 tccccaatac ttttgtataa aagatcatta aaaaagaact agcaaaaatg aaagtaaaat
      721 cgtttccaaa atctccaaac atatatccta ttttgtctct tataccaaat tttctttcca
      781 tacaattctc tcccttctgt tgttaagcgt tttcattctg atgaaagtac gtactatact
      841 ctgaagcgta aagagtcagt atttattttt tacatttcag agtattattt gttttttctc
      901 ctaataattc tttatctcat aaaacttatc tatatattta ctagctactc gacttacttc
      961 ttcaaaatca cctttttcaa cagaagccaa taaattccca cctacaccaa ctgcaacaac
     1021 tcctacttca aaccaatctt ctaagttctc tatacttact ccgccagtag gcattatatt
     1081 tacgtgtggt aatggagctt gataggcttt tacaatggat ggaccaaaag cagaacctgg
     1141 gaatagttta atgatatcta cgccagcagt aagtgcctcc tttatctcag taattgtcat
     1201 acaacctggt aaataaggaa tttgatataa attacaaatc tcagctgttt gtcgatcaaa
     1261 agatggacta acgatatatt ctgccccagc cataattgcg atacgagcag taaacgcatc
     1321 taaaaccgta ccggctccga ttattacatc tttttgatca gcatattttt tagcaatttc
     1381 tttgattatt tcttcagcat taggaactgt aaaagttagt tcaatccctt taattccacc
     1441 cgcaataatt gaatttattg ctttaatggc ctcatcctta tttttcccac gtacaaccgc
     1501 aatgactcca gccgcttcta acttacttaa aatttctact tttttcatat ctatctcctt
     1561 cctttatgga tacatacttt gtctagctgt actaacttac aaagaataaa tttaagcttt
     1621 tatttcaagt attattttct acttccgatt tcaaaaaaat taaaataatt atacgcatta
     1681 cgataacaaa tattctccac tatctgacta agatacattt catcatctgg aattcggcct
     1741 gctttaaccc actcgcttag taattgacat aatactcgac gaaaatactc atggcgagga
     1801 tagcttagaa aacttcgaga atcagtcaac atacctgtaa aattcgacaa caaactttgt
     1861 tgtgccatcg tagtcaactg ttgacgcatt ccttcagctg tatcattgaa ccaccaagca
     1921 catcccaatt ggattttttg aatcataccc ccttggaagc tttgcattgc tgttgctagt
     1981 ggaagccaat cagttggatt taatgaatat aaaattgttt ttggtaagca cccactcttt
     2041 tcagcggtat cgaacaatgc tacaacattt tgtgtcaaac tagcttgatc ccccatcgaa
     2101 tcaccaccga catctactcc aatttgttcc tttaatatac ttgaattatt tcttaaagca
     2161 tttaaatgca tttgcatcgt ccaaccatat cttgtatact cagacatcaa atgtaattga
     2221 atacctgtaa caaattgtgc aatttctgat aaatggaaat ccgtttttcc tgctaaagct
     2281 tttattaaca gattgtcgag tattttttca tctgctattt cataaaaata agtattcaaa
     2341 ccgtgatctg ctaatcgaca gccaatttga tgaaaatgat ccactcgttg gctcaatgct
     2401 tgcttcaacc cattaaagtc tttgattggt atagccgctg tttgactaag ttttaaaata
     2461 tagttcgaaa agcctgatga tttgatactc cagaccttat ctggacggaa agtaggtaat
     2521 accttgaaag aattatcctg tttagaaagc tcagtatgat accacaagtc atctgtagga
     2581 tcatccgttg tacagatggc tttcacatcc atcatctcca acaattcttt cgggcgatag
     2641 gaatccgttt gaattttttc gttagcacgt cgccaaattt ctggtgcatt cttctcagaa
     2701 attgtcaaat caatatcaaa gtaacgttgt aattccaaat gagaccattc aaaaatagga
     2761 ttacctggcg ccttctccac tgtttttaca aaagctaaaa atttttcata atcatcacct
     2821 ggaccactga tcaactcttc ttctatacca ttagctcgca tcaatcgcca tttataatga
     2881 tctccagctc ctttatcatt taaccatacc tgagtcaaat tcttgtattg cttattttct
     2941 agcaaatatg ctgggtctaa atgacaatga tagtcgataa ttggttggtg ttttgcaaat
     3001 t
//
LOCUS       DAFFSV010000190         2942 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFFSV010000190 DAFFSV010000000
VERSION     DAFFSV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.193"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..92)
                     /locus_tag="LR110_002813"
     CDS             complement(<1..92)
                     /locus_tag="LR110_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL4823729.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNK"
     gene            745..1464
                     /locus_tag="LR110_002814"
     CDS             745..1464
                     /locus_tag="LR110_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL4823730.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            complement(1601..2017)
                     /locus_tag="LR110_002815"
     CDS             complement(1601..2017)
                     /locus_tag="LR110_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823731.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2020..2304)
                     /locus_tag="LR110_002816"
     CDS             complement(2020..2304)
                     /locus_tag="LR110_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823732.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
BASE COUNT          947 a          465 c          547 g          983 t
ORIGIN      
        1 ttcttgtttc ggttattcaa ccagtaactg aacaaagcgc ttaaaagccc aacaagaagg
       61 gagcaaataa agttagtgaa cacgaaacac acctccttcc agtcgcaaga ctgtcggata
      121 ggcgacaatc aattatacaa taaaaaagca aaaataagcc taagtttttt attgccttgg
      181 ggttgttagg gcttgccctg accgcctgta aggcgcttta gaacgaagga catagttttt
      241 atcgaatctt gtcttagaac ggcttagacg agctctcagg acgccacaaa ggagtcccta
      301 atgaattatt tttggcgaag gagcctaaag gcgactgctg caagtaaaat gtgagagcgt
      361 agcgatcact ccttcttaca gcgaccatag ggagctagat tggcagttta atatcatgcg
      421 cgaacctgtt tattttaaaa agtgggtgat tttttggcgg gaaattttgc tcgcgcgcgc
      481 gcgtttattt tatttatttt ttaaagattt tttagaaccc tttttagggg gcagttgagc
      541 cttactctcc caacggatac agaagggtac taacaacttt ttatctgagt actaacaact
      601 ttttatctga gtactaacaa ctttttatct gagtactaac aactttttat ctgagtacta
      661 atatcctttt gtataataac aaccaaccta tttacgagtt ggttgttaaa ggttattatt
      721 tatttgtaag gggggaagaa aggaatggat gaaaaaacgg aattaacagt caaatatcaa
      781 aatgaattga atttagtacc gctgaaaaat ttcaatgcta agcagatgga tttatttttt
      841 gcattatgcg ctcgtatgaa agatcgtggt ttgaaaaaag ttagattttc tttcgaggaa
      901 ttaaaagagt taagtgatta taagatgact gctacaaaag cctttgtcgg tgatttagaa
      961 aagctctata aagatatgct taatcttact taccgtactg aagatgagga ttctattcgc
     1021 tactttgttt tgtttactgg ttttgaaatc aataaaacaa aaaagtttgt tgaagtcagt
     1081 gttaatccgg acctagaata tattattaat ggtttaacag ctgaatttag tagatttgaa
     1141 ttatctgctt tcacgtcgtt gcgttctacg tatgcaaaaa ctctattccg actgcttatg
     1201 cagtaccgtt ctactggtta ttatgtagtt cgtattgaag agtttaggag cttaatggat
     1261 atcccagaat actatcaaat gggtaacatt gatcaaaaag tattaaatcc tgcattacga
     1321 gaactaaaaa aatacttcaa tgatcttaaa gtcacaaaaa taaaagcaaa aaagggcaat
     1381 aaaatcgcca aattagaatt tactttttca agcctaacgt cagatttgcc aaagatttct
     1441 ctgcgtaact ggttagaaga ataaataaaa aagaactcgc cccccttaca ttaagcaagt
     1501 agacaagtcc cttacaaccg actaagattg ctagtacatt ctagcatatt gtatggggct
     1561 tctaaataaa aaacagatag gaataactcc tatctgtttt ttatcgtttt agaaaatttg
     1621 ttgggtcgaa atttctagaa gtgactgttt gactggtttc tttttgatga ttctttggtc
     1681 gatttcgatt tgatggattt agtttagctt cgattttttc tttcacttct tgatatactt
     1741 tagaatttgt gccaataaga acgcctgtat cttttagata taaatcagca aattctgcga
     1801 tgatttcagt catgatggaa atttcttttc ccatagcatt tagttttgcc tgattgctgg
     1861 tagtggtcgt gttggtatcg ggttcataac tcatacataa aaagcgaatg gcttccgagt
     1921 agttattgat atctggattt ttttcgatga tggaatctaa aacgtcaaca tgactagggt
     1981 acaaagtaac atgtttttgg acagaagttt ttctcatggt tatatcattc ctccgataag
     2041 catttcaatg atttgattat ttgtgtagac atactcgctt aatatttgag tattttctgt
     2101 ttggatagca ataaatttat cgagcaataa atcgaattgg tcttttttgt aagtcgtaag
     2161 ttgacggtca aaatcacttt cgattaaaac ttttagatat ttatttcgac tccatttttc
     2221 tcctgtttta ttcgtgagtt cttggcaacg tttgtctatt gttgcaacaa actttggatc
     2281 aatattccga actaaaatgt ccataaaaaa tctccttttg caattatgta taatttatgt
     2341 ataccataca aatactatat ttgtatagct gttttttgcc aaaatgctat acataagcta
     2401 cccacgatgg cgaaaaagtg atatcaaatt tttttctgag aaacgtgata ggcacagcct
     2461 atctacccac tttttttaga aagtgggtac acgtttcctt atgctcttta aaattcgggg
     2521 gggagtgctg cacgtgtaat cgataaccaa atttaacaga cgaaaaattg ttggaatata
     2581 aaaatatgac acccgaaaga aaacgatcgt tggaaaaatt aatgcaacta gtgaacgaag
     2641 acggcggaat ttcagacgca gcttttgacg catggagcga atttcatgga gaatataatt
     2701 aagtgggttt aaatggctct aaaatcgctt ctaagagttt tttagcttgg accaatgaaa
     2761 tacaccaaaa actaaataaa acgtcttaaa tcgaaaaata cgagctctca gagcgacttt
     2821 atttgtaaat gttgcatata agaaataatt acggatttta gacttttgta ggatattttt
     2881 gaaataaata tttatttctg ttataatttg attgcaaata agtgaacacg aaacacacga
     2941 aa
//
LOCUS       DAFFSV010000191         2913 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFFSV010000191 DAFFSV010000000
VERSION     DAFFSV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.194"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(254..589)
                     /locus_tag="LR110_002817"
     CDS             complement(254..589)
                     /locus_tag="LR110_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823733.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            complement(628..2220)
                     /locus_tag="LR110_002818"
     CDS             complement(628..2220)
                     /locus_tag="LR110_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823734.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTLEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEYGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTEREKIEYQRLNQAMEKYQAEPHKKTSGTLPGVFSISSDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEVGLKAAGRK
                     MMETLFEKEKLNDTGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QTQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            complement(2240..2662)
                     /locus_tag="LR110_002819"
     CDS             complement(2240..2662)
                     /locus_tag="LR110_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823735.1"
                     /translation="MAKATRKHIYLYDMHIEVLDEIISTHDDISNYSEAVRHLCMMYD
                     PKTEEKISPSQIKLNAIGKEVSILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            complement(2701..>2913)
                     /locus_tag="LR110_002820"
     CDS             complement(2701..>2913)
                     /locus_tag="LR110_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823736.1"
                     /translation="LSERTGTKWSRNRYLKVLIENDFDHALLDYKKDQFDRLLEKFVD
                     IQTYNTKILEEYIATNNQLIALLIEQ"
BASE COUNT          743 a          586 c          423 g         1161 t
ORIGIN      
        1 gcttttgccg ctttgtctgt gactacaaaa gctctgcctg tttgaatttc tgtcacaaaa
       61 acaagattgt ctctgctttt ttcaacaaca actccaatcg ttgaataagc catttcttct
      121 tcctcctttc ttttgatata atgtagacgg aaatcgcacc actcaatgaa ttggcggaaa
      181 acaattcagt gaaaaaaaag agtcctggac aatcacgttc aggactcttt tacttctttt
      241 attcacttaa caactaacat tctgtgagtg aaatagactc ttcaatgctt ttacacggga
      301 agttattgat cccctcttca ttcatttccc aagtagtata ataaggcgct aacttgccct
      361 catattcttg aatgataaaa tatttttcac ccttcacatg attacaacag tacacaccag
      421 tagctgggta ctcaacgact aaaaattgat ttggatcaaa ctgttcgttt atcaacattt
      481 ctcttatctg aaacaaatgc tctttttcta aacaatcggg aagttgttcg attcgttctt
      541 ctcttaatga tccttctata actttccatt gatctaaata ttgtttcatt gctattcctc
      601 ctactgtctt tactgtcttt actgtcttta ctgtctttct tgttccattt ggattcttcg
      661 ttgttgctct tcgtatgctt tacttgcgtg gtatttttct tcatgggcat taatcgttcg
      721 atttaattga cgcaaactcc gctgcgtgac aactctttgt tggaattgtt tcctgcgctt
      781 catgaattct tcgtaactat atttgtattc attcgttggc aattgtttaa gcgttcgaaa
      841 ggatcgttca ttgtaaatag attgcgtctg atcaacactt tctattaatg gttttatttg
      901 actatcctgt ctcttcagct ctgttaggat ggcatttcct gtgcgctttt ttaaatcaaa
      961 gagagcattt ttctctgcat cctttgattc tctcttgctt tccccataaa gactttttgc
     1021 ttcttctttc aatgcttttg aaatctgtat atattcttgg tattcaggtt cttttgttaa
     1081 cacatattgg gttaactcgt cgagtttctc tcttgtgtat ggtgaaagtt tagttcgatt
     1141 ttcatcccca taattccatt ccgaacgttt gtcaggtaag gttttatata cttcatttaa
     1201 taagttcatt gcgtgtcgat cataacgtac tggctctttt accacttttg ttaagctgtt
     1261 acgcaaatca cttttccgcg ttaataaatt tcgtttctcc atgcctatca ttcgatcctt
     1321 ttcataagaa aaacgttcta attcatgcac ataattggtc ttcattgcgt ctaatgttgc
     1381 ttgtcttctc tttcctttcg gtacgatata actcacacca ttttcgacct cagtgtatgg
     1441 tttacgcgta ttttcttttt caacaactgc aaaatgaata tgaatatgct tagtgttacg
     1501 gtgaatggta gccatccaat atcccgtatc attcagtttt tctttctcaa acaacgtctc
     1561 catcattttc cttccagcag cttttagtcc aacttcatct aaagtatctg ttttgggatc
     1621 gtacaatcct attttttcaa gatacgaatt atcatgagaa acaacatctt gataaatgat
     1681 tgagcctttt tgttgccctc tttggaagat ctgcttaatc tccttcagat cacttccttt
     1741 tacttcgtct gaagatatag aaaaaactcc aggaagagtt cctgaagttt ttttgtgtgg
     1801 ttctgcttga tatttttcca ttgcttgatt caaacgttga tattctattt tttccctttc
     1861 cgttagttcc tttttttgtt ctaacgcata gtgacgcgtc atataagaaa gatatttggt
     1921 aaaatcttgt tcctctaact tgtcaaaatt gattccatat tccatcaacg attgcgcttc
     1981 attcgacgtt tcactgaatt ctttatccgt cttactctcc gaaaaagttt ctccttcagg
     2041 aatataaaaa tcctctaaag cattcttgat tcgttgcagt tcctgttctt cttcgagcgt
     2101 gcgccgttcc atttcttcca gcgctttttc tcttgtcata taatcaatgt atgttgaaaa
     2161 agatttttta ttgggcaagg taaattgaca agtgagaatg actgcaggtg atttactcat
     2221 cacgttctcc ttctaatttt tatcgtttta aaaaccttgt tggatcaaat acttcacttt
     2281 ttgtagtcac tgattcgctt attgttttct ttatcttagg agaatatttc ttcgtttgat
     2341 ttgcttttat cttgccttcc acacgctctt tcgcttcttg ataaacagtt gaatttgtac
     2401 caattaatat tcctgtatct tttaaatgaa gatctgcaaa ctccgaaacc atttcagtta
     2461 aaatagaaac ttcttttcca attgcattta gtttgatttg actgggactt atcttttctt
     2521 cagttttggg atcatacatc atacacaaat gtctaactgc ttcactataa ttggatatat
     2581 cgtcatgagt agaaattatc tcatcaagta cttcgatatg catatcataa agataaatat
     2641 gttttcttgt tgctttcgcc aaagttgatt tcctcctttt ataaattcac aatattattt
     2701 ttactgttca attaacaagg cgattaattg attatttgtt gcaatatatt cttctaaaat
     2761 tttcgtatta taagtttgaa tatccacaaa tttttctaac aaccgatcaa attgatcttt
     2821 tttataatcc agaagagcgt gatcaaaatc attttcgatc aaaactttta ggtatcgatt
     2881 tcggctccat ttagtcccag tacgttcact taa
//
LOCUS       DAFFSV010000192         2862 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFFSV010000192 DAFFSV010000000
VERSION     DAFFSV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.195"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            5..2377
                     /locus_tag="LR110_002821"
     CDS             5..2377
                     /locus_tag="LR110_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823737.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..>2862
                     /locus_tag="LR110_002822"
     CDS             2438..>2862
                     /locus_tag="LR110_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823738.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYA"
BASE COUNT         1144 a          455 c          620 g          643 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgc ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgag aagatgtttg aacaccagag
      121 aaagaccaac ggtcagccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agaacgttta gaaaacaaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 cctggatatg acaatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cagaaaaagg aaaagaaaat cttcttcctg aagtacaagc
      481 aattattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 agactatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctttgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tagaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 aaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaagcaaga
     1081 agtcaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtgaaa gaacttttga attttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ctgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgggg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cttcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 ggcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctgtagaaac ggcagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtga ttttaaaagg
     2221 aaaaagttat ggaaaagaga ttcaaacggc gttaccttca gaagaatttt cgctaggaaa
     2281 tccattccga ttttcacttg aaacagtgtc agaagtggcc aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg cgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg tttgaaaatg accgaaaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagact atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ctctaagcaa caatttaaag cagccacaga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtagaa aatcttcatt ttgagacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gc
//
LOCUS       DAFFSV010000193         2800 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFFSV010000193 DAFFSV010000000
VERSION     DAFFSV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.196"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            628..2721
                     /locus_tag="LR110_002823"
     CDS             628..2721
                     /locus_tag="LR110_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL4823739.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT          913 a          494 c          560 g          833 t
ORIGIN      
        1 tatcaagagg tcttgagcct gccgtttaac tctgagccac aaggagcaat ttcaaaaaat
       61 agctccttat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
      121 gtttaccaag aggttgtaaa attagcgtcc tgacataatc taataccgag ggtcagctga
      181 ttgaacaccg tgtatcaaga ggtcttgagc ctgccgttta actctgagcc acaaggaaca
      241 atttcaaaaa acagcttctc atgttttata aatattgtga cttgtggccg aggagttggc
      301 aggagaacac cgtttaagga agaagttgtg aaaaagctgt cctgcatcaa gaaataagga
      361 gctgcgacaa gaattttgtc acagcctctt tttttgtacg aataccgaga aactggcttt
      421 tgaataccgt ggagtgaagc ggtcaccaca agacttttat ctcttcatta gtttaatcgc
      481 ttacatcgca gcggttttat gctgtgaaaa gtggacggaa taatctttag aaagaataaa
      541 aaaatcttaa gataacaact tttttaggaa attttcattt ttcaggagca aaataagaaa
      601 aaatgtgaat agagagaagt gatatacgtg acacaactta aaaaattgtg ccaaaatcgt
      661 ttagcaatcg ttttgacagc tgttttcttt ttctggctga agacgatcat tgcctactat
      721 gcagattttt cattaggtgt ggaaggtacg atccagtact ttatcttgtg gatcaaccca
      781 attgcgacta ccttattgtt ttttggcctt tctttgtata ttaaaaagcc aaagccagcg
      841 ttagctattc ttttgatcat tgatatttta aatacgcttt tgttgtactt gaatattatt
      901 ttttatcggg aattcacaga tttcattaca gtcaaatctg tattaggctt ttcgaaagtg
      961 tcgcaaggat tgtcaggaag ctccttttct ttgatgaagc cccatgatgt catctactgg
     1021 ctggatatcg ctgtgttcat tgggctactg gtttggttga aggtcaagaa aatacctatc
     1081 aaaagcaacc ctgtcggcaa accgatggct atcgctgtta cctgtctttc agggcttatt
     1141 ttttcaggaa atctagcgct gagcgaagcc aaccgtccac aattgctgca gcgaacattt
     1201 gaccgttcat atattgtcaa atatctagga atcgatgcgt atactattta tgacggaatc
     1261 aaaacaggaa tgactagcag tgttcgcgcg catgcaagca gtaatgggat tgatgaagtt
     1321 ttagattata caaagaaaca ttatgcagag ccaaatcccg aaacatttgg gatcgctaaa
     1381 gggaaaaatg tgatcgttct ccatttagaa agtttccagc agtttttgat taatatgaag
     1441 gtagacggac aagaagtcac accgttttta aatagtattt tccagaatca agcaacgatc
     1501 agctttgaca atttctttca tgaagtagga cagggaaaga caagtgatgc tgaaaatatg
     1561 ttggaaactg gcacatttgg tttacctcaa ggctcattat tcacagaact tgggtcagac
     1621 aatgtctttc aagccgcacc agcgatcctt ggacaaaagc aaggctatac aagcgctgtt
     1681 ttccatggga atgtagctag tttttggaat agagatcatg tatataaaaa cttaggttat
     1741 gacaatttct ttgatcgatc ttattttgat gaatccgacg aaacgttagg ttatgggata
     1801 ttagataaag atttattcag agaatctgcc caatatcttg agcatctaca acagccgttt
     1861 tacacgaaat ttctttccgt aaccaaccac acgccttatt atacggatga caagaatttc
     1921 gactttccat ctttgaatac tggcaatagc acagtagata attatgttcg aacggcacat
     1981 tatctggatc aatcattgga acaatttttt acgtatttga aaaaatctgg tatttaccaa
     2041 aattcaatct ttgtgattta cggggatcat tttgggatct cgaatacaga taataaagat
     2101 ttagcaagcg ctcttggtaa agatccagat acatgggatg aatttgacaa cgcacagatg
     2161 caacgagtgc cattgatgat ccatatgcct ggttatacta aagggacagt caatcatgaa
     2221 tacggtggag aaatcgatgt tttgccgaca ttactacatc tattgggaat cgatgataaa
     2281 gattatcttc attttggtac agacttatta tcttctcagc atgatcaagt agttgctttt
     2341 cgaaatggga actttgtgac acccaaatac accgtcctag gaggaaaagc ttacaataat
     2401 cagacaggag aaatcatcga tacgaaagca gcaggaatcg atgaggaaat ctccaaagat
     2461 caggaaaaag tcaagactgc gttaagtctt tcagataagc tgaatcagga aaatctcttg
     2521 cggttttatg tgccaaacgg atttgagacg attgatccga aaaaatatga ctataaaaat
     2581 gaagcggaac gtaatgaagc aatcgaaaag caaaaaggcg aacattcgac aagtgtcttt
     2641 tcaaaaaatg gtaataaaac aacaaccaac ttatatccag taacaagaac agacgttgaa
     2701 cagcaaaaaa ataatcaata aacaatcaaa ccaaccttca gtagttagac aacattctaa
     2761 tgcttgaagg ttggtatttt ttatgaaaaa aacatttttt
//
LOCUS       DAFFSV010000194         2785 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFFSV010000194 DAFFSV010000000
VERSION     DAFFSV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.197"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(1273..1938)
                     /locus_tag="LR110_002824"
     CDS             complement(1273..1938)
                     /locus_tag="LR110_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823740.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..>2785
                     /locus_tag="LR110_002825"
     CDS             2064..>2785
                     /locus_tag="LR110_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBL4823741.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCL"
BASE COUNT          891 a          436 c          494 g          964 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttatt
//
LOCUS       DAFFSV010000195         2774 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFFSV010000195 DAFFSV010000000
VERSION     DAFFSV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.198"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..268)
                     /locus_tag="LR110_002826"
     CDS             complement(<1..268)
                     /locus_tag="LR110_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823742.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERY"
     gene            500..1234
                     /locus_tag="LR110_002827"
     CDS             500..1234
                     /locus_tag="LR110_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4823743.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            1273..2415
                     /locus_tag="LR110_002828"
     CDS             1273..2415
                     /locus_tag="LR110_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL4823744.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         1055 a          447 c          466 g          806 t
ORIGIN      
        1 tataccgttc catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt
       61 ttataacagg taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta
      121 gatattcact tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt
      181 ctagcatagg ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa
      241 caatgctatc aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa
      301 attttctaag aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg
      361 attaatcaaa caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa
      421 aggggtgtca atagttttgt aaaatttatt tacaacaccc cttttttgag gtatactaat
      481 tttaggaggt gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa
      541 gatactcgga acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt
      601 tggtcttatg attaacaacg gtcaattaat tgacaaaaag actatctacg aatgggagaa
      661 gggaacctat ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag
      721 tatagaggag ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag
      781 agatagcatt gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg
      841 tcaacagttc ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt
      901 agaaccagag atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa
      961 aattagtctt ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaga
     1021 atttgacaat aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt
     1081 agatgaatgg ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga
     1141 aattactgat aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac
     1201 taagaaaaaa atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga
     1261 ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg
     1321 atgtataaag gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga
     1381 agaggtttta gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata
     1441 ttatacggtg ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg
     1501 ataactcatc agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca
     1561 aataatcaaa tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat
     1621 tgtcaaaaag tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa
     1681 cagactgcac aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg
     1741 gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa
     1801 gaggagctta ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc
     1861 ttcttcagat tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg
     1921 aaagacatag acacgtttaa taaagaacta catgtcaata aaactcttgc tgtagatgaa
     1981 tttggtaaag ttataattca atctccgaaa acaagagctt ctagacgagt tatctcttta
     2041 gatactgaga ctctttctat cctcaacaac tggaaattac agcaaaaaga agagtattta
     2101 aaacttggat acaatactag ctctaaagag caacatgttt ttacaacagt taaaaacact
     2161 ctatacattc caaacactgt taatgattgg ttaagatata ttttgaaaaa atataacttg
     2221 cctcgtatta cacctcatgg gtttagacat acacatgcta gtttattatt agaagcagga
     2281 gaatctgtca aggtagttca acaacgatta gggcatgaaa atagtaaagt gacattagac
     2341 atctatgcac atattacaaa taatgcacct aagaaaacag gtcaagactt tgctgatatg
     2401 atggctcatc agtaaaagag ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca
     2461 aaaaagctag aacccttata tatcaagggt tctagctcct ataacaggtt tacccaacac
     2521 tgccttccaa tgactctttt gggaatcaga ggaaagaatc aggctttata gagatatttc
     2581 atctaacaaa ttactttaga ctgacagtat atatcagcag gtactgtcat cgggaacgta
     2641 gccaatatcg tatccaaaac agcaggtaga gaagctattt gaggattcaa gttattctat
     2701 ttcaaccatg aagttaagtt cattaaaaat aaagatagtt ggtatttcaa taatttttgt
     2761 atagagttaa tcac
//
LOCUS       DAFFSV010000196         2741 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFFSV010000196 DAFFSV010000000
VERSION     DAFFSV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.199"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(178..1197)
                     /locus_tag="LR110_002829"
     CDS             complement(178..1197)
                     /locus_tag="LR110_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBL4823745.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1194..1418)
                     /locus_tag="LR110_002830"
     CDS             complement(1194..1418)
                     /locus_tag="LR110_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL4823746.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1415..1708)
                     /locus_tag="LR110_002831"
     CDS             complement(1415..1708)
                     /locus_tag="LR110_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823747.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(1745..1882)
                     /locus_tag="LR110_002832"
     CDS             complement(1745..1882)
                     /locus_tag="LR110_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL4823748.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(1884..2315)
                     /locus_tag="LR110_002833"
     CDS             complement(1884..2315)
                     /locus_tag="LR110_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823749.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(2329..2616)
                     /locus_tag="LR110_002834"
                     /pseudo
     CDS             complement(2329..2616)
                     /locus_tag="LR110_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          766 a          566 c          445 g          964 t
ORIGIN      
        1 acataaagcc ataaacaatc aaccagtaga atgtgtggca acaaacctgt tatcgcatag
       61 cttggagtgt gactattatg ggtgatagtg aagatatgcg ataacatcac tattttatcg
      121 aatgattttt atagttgtca atatagttat gtaatgctcc tcaacgagga gctattttta
      181 tcgtttagga atatttaaat accaacgttt gtcatggaaa tcttgcgcac cgcctttagt
      241 gtttccctct ggatcattcg ttgcccgcat catgacatag actttcttat tagggaagtt
      301 acgcatgtta aaagatacat gatagccaac atttccagaa gtattataag cttgatttac
      361 atctgatcta taaattccat cagctcttac tcgagctaat tctttcccag tattgtaatc
      421 cataatgaag atatactcgt atttatagtt agcaatgtgc catccagcta catgcaaatt
      481 tgcattttcg atttctccaa actgatcaat gtgggcgtaa ttcgttccat ctgtcagtgt
      541 aggattagct gcaccagctc gtgttggatc aatgacaggc ttgttttcag aagttgttgg
      601 attttcatcg gtaaatccat gagctaaatc atatgctaat ttttctttac ttacgcccat
      661 ttcagaaaga taaccgtaag gatctgtatg atcgccccag atgttttgtg ttacccataa
      721 atgcgatttg attcccggtt ggttataagg agtgtccaac gttaatggaa taccatattt
      781 cattgctgaa tctctagcca attcaacgta tgccttgtag tttttctcaa acgttgcttt
      841 atcatgtgtg tgttgtaact caatctgcac aggactgttg gcattagcat acgaaccagc
      901 accgtactgt acataaccag gttgaccgac ttgataaaca attccgccgt ctcccacaat
      961 ataagcagta taagcgctag tccatgaacg ttgcatatac tgcgcttcat tgcgtcctgt
     1021 tgctgtttca ttagccgttt catgcagtaa aatatactga ttatttgcta cttgtgagct
     1081 accttcattt gcgcccaaat taaattcatt gttgatagta taggcaaatc cgttaattgg
     1141 caataaaaaa agagccgtta acaggctcat cgcagtaata gtaattttct ttttcatttg
     1201 tttcctcctt cttcgctttc agccgagaac attttgtagg ttcgatttga tacacccaac
     1261 acactcccta aaaacgcgcc aaaaccagta atgatgacaa cacagatatc tgtgtactgc
     1321 caattgagcg ctttaccaac taaccccacg aaagtagcta gtgcgggaat aattaccagt
     1381 gcgaaccatt ttagtacttc gaacgtttta ttattcattt tcttctctcc ctaaacaaag
     1441 ttttgatttg ttgcgtgtgt tccaccaatt tttctgtatg tgtatctaat ctttcatcgt
     1501 gtttctttag ttcttcatga atcatcaatc gatctgattt gctcgattct aaatctttag
     1561 tcagcaaatc taaattgtgg cttacttttg aaagagtctc agtaattttc gagaaagatg
     1621 cagcaatcgg tctaattact aataaaatca aagaaacgat agcggttatt gatcctgcta
     1681 tcattcccca ttcccctaaa ttaatcatgt gacaactcct ttaccttaaa taaaaacgca
     1741 tcaattaaga tgcgctctta tctttattaa tgattttatc tgcttcttcg tctgtaatgc
     1801 ataatggaac gaaaaccatt acttgttcgt tagtgaaaca gccccagtca tacattagtt
     1861 tgatatcttc atatgaatac atgttacttc ccaccttgta attgtttttt gatttcttcg
     1921 atttccttgg tattttgtac agaagcaagc atcgtttttg aattcaactg cgctaaactt
     1981 tctgactttt ctttcaataa gacattttct tgcttgattg ctacattgtt tagcattgct
     2041 ttagaattta gctgtttcaa ttcatcattt tctgccttca gcgtttcgta taatgcctta
     2101 atattagcca attcatctac gctatcaaca gcattccctt ttttctcttc ttcagtagct
     2161 aactcaaccc actgtttttt atcaaaatca aatcttggtc tccagttagg aactggtggt
     2221 ttaatctctg tacagttttc agggatattt tcttgattgt tcaaaatgat ttgctcgaat
     2281 ccgtaaggct tgattgattt gtaaactact ttcattagat ttcctccctt aaattgaata
     2341 agtgataaca aatgaataag ctgatccata acttgaattt cttctccatt taatggctcc
     2401 gtctgcgcca atagatagtt gagcactgtt caaagtagaa cggtctattg acccaaccag
     2461 ttgttcaaaa ctaattggtg tccgatagcc ttctggaaca gttaaaataa ccgaatcatt
     2521 cccgccacta ctttttccgt ttaaagccac aaaatatata gaaactgttt ttccttcacg
     2581 ataaagcttt gctgtccctg tattaccgtt agtgacaacc agatttacca cttcatatct
     2641 gttatcatca gtcgtcaaaa cacgtttatt attgattgtt acagaacctg aaaaattctt
     2701 atgtcctgca atggcttggt ctccatctgt ctgcaccaat g
//
LOCUS       DAFFSV010000197         2682 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFFSV010000197 DAFFSV010000000
VERSION     DAFFSV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.200"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            34..456
                     /locus_tag="LR110_002835"
     CDS             34..456
                     /locus_tag="LR110_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823750.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            476..2068
                     /locus_tag="LR110_002836"
     CDS             476..2068
                     /locus_tag="LR110_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823751.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2089..2424
                     /locus_tag="LR110_002837"
     CDS             2089..2424
                     /locus_tag="LR110_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823752.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
BASE COUNT         1074 a          407 c          546 g          655 t
ORIGIN      
        1 gtattgtgaa tttataaaag gaggaaatca attttggcaa aagcaacaag aaaacatgta
       61 tatctttacg atatgcatat cgaagtactt gatgaaataa tttctactca tgaagatata
      121 tctaattata gtgaagcagt cagacatttg tgtatgatgt atgatccaaa aatcgaagaa
      181 aaaataaatc caattcaaat taaattgaat gcaatgggaa aagaaatttc tatattaacc
      241 gaaatggttt cagagtttgc ggatcttcat ttgaaagata caggaatact aattggtaca
      301 aattcaactg tctatcaaga agcaaaagag cgtgtggaag gcaagataaa agcgaatcaa
      361 acgaaaaagt attctcctaa gatcaagaaa ccaataaatg aatctgttca tgccagagta
      421 gacccgtttg atccaacaaa gtttttaaaa agataaaaag tagaagggga acatgatgaa
      481 taaatcgcct gcagtcattc tcacttgtca atttaccttg cccaataaaa aatctttttc
      541 aacatacatt gattatatga caagagaaaa agcgctggaa gaaaaggaac gacgcacgcc
      601 ggacgaagaa caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga
      661 aggagaaact ttttcggaga gtaagacaga taaagaattc agtgaaacgt cgaatgaagc
      721 tcaatcgttg atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa
      781 atatctttct tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag
      841 ggaacaaaaa gaataccaac ggttgaacca agcaatgaaa aaatatcaaa cagagccaca
      901 taaaaaagct tcaggaaatc ttcctggagt tttttctatg gcttcagacg aagtgaaagg
      961 aagtgatctg aaggagatca aacaaatctt ccaaagaggg caacaaaaag gctcaatcat
     1021 ttatcaagat gttgtctctc acgacaattc gtatcttgaa aaaatgggct tgtacgatcc
     1081 caaaacagat accttagatg aagaaggatt aaaagcagct ggaagaaaaa tgatggagac
     1141 attattcgag aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa
     1201 cactaagcat atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaacctta
     1261 cacggaggtt gaaaatggtg tgagttatat cgtaccgaaa ggtaagagaa gacaagcaac
     1321 attagacgca atgaagacca attatgtaca tgagttagaa cgtttttctt atgaaaagga
     1381 tcgaatgatt ggtatggaga aacgaaattt attgacgcgg aaaagtgatt tgcgtaacac
     1441 cttaacaaaa gtggtaaaag agccagcgcg ttacgatcga cacgcaatga acttattaaa
     1501 tgaagtatat aaaaccctac ctgacaaacg ttcggaatgg cattatgggg atgaaaaacg
     1561 aaccaaactt tcaccataca caagagagaa actcgatgag ttaaccaaat atgtgttaac
     1621 aaaagaacct gaatatcaag aatatataca gatttcaaaa tcattgaaag aagaagcaaa
     1681 aagtctttat ggggaaagca agagagaatc aaaggatgca gaaaaaaatg ctctctttga
     1741 tttgaagaag cgcacaggaa atgccattct aacagaactg aagaaacagg atagtcaaat
     1801 aaaaccttta atagaaagta tggatcagat gcaatctatt tacaatgaac gatcctttcg
     1861 aacgcttaaa cagttaccaa cgaatgaata caaatatagt tacgaagaat tcatgaagcg
     1921 caggaaacaa ttccaacaaa gagttgtcac gcaacggagt ttgcgtcaat taaatcgaac
     1981 gattaatgcc catgaagaaa aataccacgc aagtaaagcc tacgaagagc aacaacgaag
     2041 aattcaaatg gaacaagaaa gacagtaaag acagtaggag gaatagcaat gaaacaatat
     2101 ttagatcaat ggaaagttat agaaggatca ttaagagaag aacgaatcga acaacttccc
     2161 gattgtttag aaaaagagca tttgtttcag ataagagaaa tgttgagaaa cgaacagttt
     2221 gatccaaatc aatttttagt cgttgagtac tcagctactg gtgtgtactg ttgtaatcat
     2281 gtgaagggtg aaaaatattt tatcattcaa gaatatgagg gcaagttagc gccttattat
     2341 actacttggg aaatgaatga agaggggatc aataacttcc cgtgtaaaag cattgaagag
     2401 tctatttcac tcacagaatg ttagttgtta agtgaataaa agaagtaaaa gagtcctgaa
     2461 cgtaattgtc caggactctt tttttcaatg aattgttttc cgccaattca atgagtggtg
     2521 cgatttgcga ctacattata tcaaaagaaa gagaagggag gaagaagaaa tggcttattc
     2581 aacgattgga gttgttgttg aaaaaagcag agacaatctc gtttttgtga cagaaattca
     2641 aacgggcaga gcttttgtcg tcacagacaa agcggcaaaa gc
//
LOCUS       DAFFSV010000198         2622 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFFSV010000198 DAFFSV010000000
VERSION     DAFFSV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.201"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            7..1398
                     /locus_tag="LR110_002838"
     CDS             7..1398
                     /locus_tag="LR110_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL4823753.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="LR110_002839"
     CDS             1414..2454
                     /locus_tag="LR110_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL4823754.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..>2622
                     /locus_tag="LR110_002840"
     CDS             2473..>2622
                     /locus_tag="LR110_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBL4823755.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHS"
BASE COUNT          908 a          412 c          487 g          815 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt ca
//
LOCUS       DAFFSV010000199         2606 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFFSV010000199 DAFFSV010000000
VERSION     DAFFSV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.202"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..876
                     /locus_tag="LR110_002841"
     CDS             <1..876
                     /locus_tag="LR110_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL4823756.1"
                     /translation="ILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQ
                     PGYVQYGAGSYANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTP
                     YNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGA
                     QDFHDKRWYLNIPKR"
     gene            991..2199
                     /locus_tag="LR110_002842"
     CDS             991..2199
                     /locus_tag="LR110_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823757.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            2183..2353
                     /locus_tag="LR110_002843"
     CDS             2183..2353
                     /locus_tag="LR110_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823758.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT          958 a          384 c          467 g          797 t
ORIGIN      
        1 atcctattgc atgaaacagc aaacgaaact gcgacaggac gaaatgaagc gcagtacatg
       61 aaacgttctt ggtacaatgc ctatacagcg tatattgttg gcgatggtgg gatcgtttac
      121 caagttggac aacctggtta tgtacaatat ggcgctggtt catatgctaa tgcaaatagt
      181 cctgtgcaaa tcgaactgca acatacgcac gataaagtaa cgtttgaaaa gaactacaag
      241 gcatatgttg aattagcgag agattccgct aataaatacg gtattccact tacattagac
      301 acaccgtata atcaaccagg aatcaaatca catttatggg taactcaaaa catttggggc
      361 gatcacactg atccgtatgg atacttatca gaaatgggtg taagtaagga aaaactggct
      421 tacgatttag ctcatggatt tacagacgaa aacccaacga cttctgagaa caagcctgtc
      481 attgatccaa cccgagcagg tgcagcaaat ccaactttat cagatggaac gaaccattct
      541 cacattgatc agtttggaga aatcgaaaat gcgaacttac acgtcgctgg atggcacatc
      601 gctaactata aatacgagta tattttcatt atggactaca atactgggaa agaattagct
      661 cgagtaagag ctgatggaat ttatagaccg gatgtaaatc aagcttataa tacttctgga
      721 aacgttggct atcatgtatc ttttaacatg cgcaatttcc ctaataagaa agtatacgtc
      781 atgatgcggg caacgaatga tccagaagga aacactaaag gcggtgcgca agatttccat
      841 gacaaacgtt ggtatttaaa tattcctaaa cgataaaaat agctcctcgt tgaggagcag
      901 tacatagaat tgaaaattaa cgttaaatca aaaaaatatt tacaagatgt ttatttaagt
      961 agataataaa agaaaaaagc aggtgaaatt gtgacagcag aaattggtat tatgaataaa
     1021 agtggaatcg tgttagcttc agattcagca tctacgattg gagatagcaa agtatataat
     1081 actgcaaaaa aattatttac tttggattcc atgcattctg taggtatcat gatatatggt
     1141 aatgctgaat ttaatggtat tccttgggaa ataatcatta ctcaatataa gaaaagcatt
     1201 ggtagttctg tttttaatac tttggaagaa tacgcggata attttataga atttgtcaaa
     1261 acagcatctt ttattagaag tgaacagacc gagcaagaac aaatgattgg ggtttttcaa
     1321 aaaataattt caggtttatt tgagagtata gaagtagata ttaacttttt aataagccaa
     1381 ggtacacaaa tagacaaaga tgtcctagtg aagttattac aaacaaaaat gaataccaat
     1441 ttgtctcaac agtcccagac attcatttta gatattgaaa aggcgttatt tcttagtaac
     1501 tatggagaaa tattaaaaga tattcttaat agtatttcta caatggaagg tgtttcagaa
     1561 gctatatcag aggaaattca aagttacgtt tatgaaatta taattagaga cgatgtttat
     1621 tcttccccta caggaatagt tatagcagga tatggaagaa tggatatttt ccctaaacta
     1681 tattcttata atatgtttgg atttgttatg aatattctga aatactcaga atatgaaagt
     1741 gctcaaatag gaaatgacaa tggttcatta agatctacta tattaccatt tgctcagtct
     1801 gatgtggtaa atactgtagt tcaaggggta gatccacaaa ttactaatta tctgtcgagt
     1861 caagtcgata gttttgacga taacggaaaa aatacctata ttaatattat aaagaatatt
     1921 tcagagtttc aacaaaatca attcatattt ccgttactaa acatgattgc tttactccca
     1981 gttgaagaaa cagcaattat cgcagagact ttattaaatt taaccagttt taaacgaaaa
     2041 tatactactt ctgtagaaac agtaggtggc ccaattgatg ttttagctat tacacctaat
     2101 gatggcccta tttggattaa aagaaaacat tattttgata ttgataataa tattggatac
     2161 agattgcgaa aggagagagc gaatgattac aataattaaa cctcaggaaa aatcccctgt
     2221 tttgaaattt aatgagagca atctagccca taataaaatg agtataactg atacagtatt
     2281 tgatgtaatg gttgagcaac aaaaaaaaat taataataac aatagacaaa gaaaagaaat
     2341 taaccaatat taatgttttt tctgttgcgt tttttgcagt cataatttaa aacctcgctc
     2401 aatcttgggc gaggtctttt tttatttttc tgaaaagtta aagtaaataa tagcataaaa
     2461 taatatattt tacaaagaat aagtacaatc tagttttttg ctattaaatg tgtaataata
     2521 aatatgccat cacaacatga agaatgaaat ccattattat ctagtctagt ccattctttt
     2581 tgtttgcagt atttgtgatg gctttc
//
LOCUS       DAFFSV010000200         2580 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFFSV010000200 DAFFSV010000000
VERSION     DAFFSV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.203"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..544
                     /locus_tag="LR110_002844"
     CDS             <1..544
                     /locus_tag="LR110_002844"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL4823759.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="LR110_002845"
     CDS             complement(1017..1589)
                     /locus_tag="LR110_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4823760.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="LR110_002846"
     CDS             complement(1605..2210)
                     /locus_tag="LR110_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBL4823761.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(2408..>2580)
                     /locus_tag="LR110_002847"
     CDS             complement(2408..>2580)
                     /locus_tag="LR110_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL4823762.1"
                     /translation="RPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSV
                     CTEIKVLLGIPA"
BASE COUNT          804 a          511 c          404 g          861 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta gttggttctg ttgcaaagtt ttaaataaag aataaaatcc cttacggtat
     2401 ctatgattta agctgggatt cccaataata ccttgatttc agtacagacc gaaaacccga
     2461 agagagtgcc ttcttttcgg gttttcttat ataatcctcg aatggcttcc atgcctttaa
     2521 tcgtggtaga ggcagtgcgt aaacttcgat agaatttatt gcgtctcttt actggacgat
//
LOCUS       DAFFSV010000201         2576 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFFSV010000201 DAFFSV010000000
VERSION     DAFFSV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.204"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            202..>2576
                     /gene="esp"
                     /locus_tag="LR110_002848"
     CDS             202..>2576
                     /gene="esp"
                     /locus_tag="LR110_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBL4823763.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVK"
BASE COUNT          935 a          392 c          523 g          726 t
ORIGIN      
        1 tcagctgctg aatggttaaa aagcacaaat atccgaaaaa aacatgaaga aatgtacttt
       61 ttttgttgta atgtgcatga aattctttaa gtaaactagt taacataagt ttagtaagat
      121 tcctattctg tgaataatac gtatcagatt tatcttttat tcttactaag aaaataacta
      181 ttaagggagt tgatttgaat aatggttagc aagaataata agagagtatt tctagaaaaa
      241 acaaagaaac gagtattgaa gtacagtatc aaaaaattaa gtgttggggt tgcatctgtt
      301 ttggttggtg ttggtcttgt cttgggaaca actgaactag ttcaagcaca agatgaaatt
      361 tctccaagca ccccacttga aacagcgata agctccgtac aagtaggtga caaagtagct
      421 tcggggaata cttttcagga gaatcctggt tatacgaaaa attataactt ttcggattta
      481 caatttagtc ctcaagaact aactggagac acactaaaag gcaatacaat tggatttgaa
      541 gtttacggaa aacataatat tgcagcatca actaaaaact gggaaatccg tcttcaatta
      601 gatgaacgat tagctaaata tgtagaaaaa attcaggtcg atccaaagaa aggaattgga
      661 tcttctagac ggacctttgt aagaattaat gattcgctgg gtagacctac aaatatttgg
      721 aaggtcaatt atattcgtgc aagtgatgga ttatttgctg gggctgaaac aactgataca
      781 caaactgctc ctaatggggt aattacgttt gaaaagagct tagatgaaat ttttaaagag
      841 ataggcatag acaatctcaa gaccgatcgt ttaatgtatc gaatctactt ggtaagtcat
      901 caagacgatg ataaaattgt accaggtatt gatagtacgg gctacttttt aacagattca
      961 gatgatttct ataatagctt agatgtatct gaaaataatc ctgaccagtt taaacatgga
     1021 tcagtaagtg caaaatatga ggaacctaat actcaaacaa aagatggttc aggatccaca
     1081 ggtgctaatg gagctataat cttagatcat aagttaacga aaaattataa tttctcatat
     1141 tcagcttccg caaagggcac gccttggtat gctaattaca aaattgatga acgattagtt
     1201 ccatatgtag cgggtataca gatgcacatg gttcaggctg acaaagtaac atatgatgtt
     1261 tcttttgaat cagggaaaaa agtggctgat ttagcaattg aacggcgcaa agatcatgaa
     1321 aattatggtg taggttcaat tactgataat gatcttacca aacttattga ttttgctaat
     1381 gcaagtccac gtccagtcgt tattcgatat gttttacaat tgactaagcc attagatgaa
     1441 atcttagaag atatgaaagc aacagcacaa gttgaagaaa ataaaccatt tggtgaagat
     1501 ttcatctttg attcttggtt gtcggatacg aataaaaaat taatccagaa cacttatgga
     1561 acaggttatt attatttgca agatattgat ggtgatggaa accctgacga taaagaagag
     1621 agcggagaca cgaatccata tatcgggaaa cctgaattag aagaagtata tgatgttgac
     1681 acaacagtta aggggaaagt attcatccac gagttagcgg gaacaggtca caaagcccaa
     1741 cttgttgata aagaaggtac tgtattagca gaaaaaacta tcgctccaaa tgaaaaagat
     1801 ggggctccaa tttcagatac tgtagaattt gaatttacgg gtgtagattc aagtaaatta
     1861 atcgcgaaag atgaattaaa aatccaaatc gtttctccag gttttgataa accagaagaa
     1921 ggttcaaccg ttattaagga atcaccaaaa gcggttgata aacaaaccgt ggtagttgga
     1981 tttaaaccag atgctaaaga atcaattcgg aataataaaa acttacctga agatgcagag
     2041 tattcatgga aaacagagcc tgatacttct aacgttactg atagtacgaa aggtattgta
     2101 actgttaaga tcggaaatcg aactttcgac gtggatgtag agtttgctgt aaaagcttct
     2161 caagctatgg aaaatgatgc aacatacgta cctataacaa caactccaga aacgacagtt
     2221 caaagtggaa aacctacatt tgataaacca gatgttcctt tagctaaaga tgccttttca
     2281 attttagatg tctataataa ggacttcggt aatgcaagtg ttgatgcaaa tactggtatt
     2341 gttacattca ctccagctaa aggtgtagga gaatcggagc cgattactgg aacaattcct
     2401 attaaaattg tttaccaaga tggttctgta ggcacgaccg atttagcagt aactgtaagt
     2461 aaagatattt atgaaaatcc aggagaaaac attcctgcag gctaccacaa agtgaccttc
     2521 accgcagggg aaggaacaag tattgaaagt ggaacaacgg tctttgcagt gaaaga
//
LOCUS       DAFFSV010000202         2570 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFFSV010000202 DAFFSV010000000
VERSION     DAFFSV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.205"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            191..547
                     /locus_tag="LR110_002849"
     CDS             191..547
                     /locus_tag="LR110_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL4823764.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            557..1456
                     /locus_tag="LR110_002850"
     CDS             557..1456
                     /locus_tag="LR110_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBL4823765.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            1825..>2570
                     /locus_tag="LR110_002851"
     CDS             1825..>2570
                     /locus_tag="LR110_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBL4823766.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT          891 a          449 c          498 g          732 t
ORIGIN      
        1 caggttgctt tttgggtctt tttccgttca agaaactgaa cgaagaaaag ggaaagttgt
       61 ctcttttgtg ataagagaaa tcataataaa cgaatgactt actttccaat aaattgagaa
      121 cagtcagaac cagacaaatt agttgcatct gatgaataaa gtgattattc tatgacatga
      181 ggtgatacag atgaaattag aagtgacaaa agaagcacaa gaagtattga agaataaatt
      241 agaagcagac gatcaacttt tattagatat cgaaaacggt gacggtccat ttgcagaaag
      301 ccaagtcagt tgccaattgg atacggcatt tcgattgatc attgtcaaaa aagatacaca
      361 aacagatcta agtctttatt ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa
      421 agacagtgca atactttaca tggatgatcc atccacactt gctttagaac caacttataa
      481 tagtttacaa ttaaaaggac caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa
      541 agactagagg gataaaatgg gaaaattggc gatcatcagc gacctacatg tggacatcaa
      601 taaattcggc gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac
      661 tcggcttcat ctagccggag atacggctaa taaagtagac cgttgcctag ctgttgtcga
      721 atttttcgtc aataaaggat tatctacgac atttcatttc ggcaatcacg agttggcaga
      781 tgtgcccaat gaacagatga tggaccattt tccagaccca cattttttaa atgaacggta
      841 tattgcttta aatgagcaga ccgttctttt agggatgaat ggctggtacg actatcagtt
      901 ttccgaattg aaagattacg aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg
      961 gttcatccag cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa
     1021 aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg attttatcca ctcatttcgt
     1081 tcctaaacgg gcatttatca tgtatcaatc tgctccttat gagcgttgga acaaattaaa
     1141 tgcattttta ggatcagaat cattcggaga attacttgac cgttattcaa atatcaaaca
     1201 agtagttttt ggccatacgc atcgtcgctt tgaagataaa tccatcggtc acacgattta
     1261 cagctgcaga ccttttggtt attattatga atggcagctg acccgagatt ttgtcttgaa
     1321 agaacagttg ctagacagct atgatcctat gaaattacgt gtcctcttgc gtgcccatca
     1381 cccagctttt caggcctatc aaacacaaca tttgcaagag gaatttgaac aagcgatgac
     1441 aatcgttccg tattgagaca gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata
     1501 aggataagta agagcagtag atccatttat cgtggatatg ctgctttttt tgcggaaaat
     1561 aatcagcgaa tgtttatttg atcggatgat ttgtaactcg cagagtacta gatgcaagcg
     1621 tgtattcttt ttttgaacga taatcacaaa aaaaaatata atatacattt tttatttaat
     1681 tataattaat attgattgaa gtctaggcag tcaagtcgag agtgtaaaat attttgtgta
     1741 aatgaaaaaa tccatacaaa aaaggaagtc gcttctgtag aataaagtta acgacaacca
     1801 attcacagaa aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta
     1861 gtcactaaag gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca
     1921 ctcctacgga ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt
     1981 aattcaggta attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa
     2041 ttaacattgg aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc
     2101 tacaaaagaa caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt
     2161 acgatgtctg aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa
     2221 accatgtcca acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc
     2281 ttaaatgata agtatgtcgc tatttttatg gacgctactt acattccact aaaacgtcaa
     2341 accgtatcca aagaagcgat ttatattgcc attggtatac gagaagacgg cactaaagaa
     2401 gtactgagtt atgcgattgc tccaactgaa tcaacatacg tttggaatga gctgctacag
     2461 gatattaact ccagaggagt tcaagaagtc ttgcttttta ttacggacgg cttaaaaggc
     2521 atgaaagata ctatccatca aatttatcct aaagcaaaat atcagcattg
//
LOCUS       DAFFSV010000203         2564 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFFSV010000203 DAFFSV010000000
VERSION     DAFFSV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.206"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(1..111)
                     /locus_tag="LR110_002852"
                     /pseudo
     CDS             complement(1..111)
                     /locus_tag="LR110_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            352..534
                     /locus_tag="LR110_002853"
     CDS             352..534
                     /locus_tag="LR110_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823767.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            518..703
                     /locus_tag="LR110_002854"
     CDS             518..703
                     /locus_tag="LR110_002854"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBL4823768.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            946..2466
                     /locus_tag="LR110_002855"
     CDS             946..2466
                     /locus_tag="LR110_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL4823769.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
BASE COUNT          874 a          434 c          513 g          743 t
ORIGIN      
        1 gactaagaaa cgttccatcg actcttcatt ttggaactgt tccttgcggt ggctgtattt
       61 cttgatttgt ttattgaaag attcgattaa attagttgag taaatgcttc gtacgaacat
      121 agttgattta ttcaccatac ttcccattac atcgtaaaac gctatattta agatttcaaa
      181 caactatatt aataggaaga aaacttcatt tttcctctta gttctataaa aagtgtggct
      241 aatttgaggc aaaatattaa attaaatgta agcgtgtcat aggatagtat ctcgtacgcc
      301 gattatcttt cttctataat taaactatca aatatagaag gagaaaaacg aatgaataaa
      361 aaagatattg taccagttca attttcaagt gcggctcaaa aatcgcaaat tgacaccaag
      421 ccaaaactag cttgcagaat caaatcggaa gataaggaaa tatttattta taatggaatg
      481 gataaatatc ttttgtatac cttgttaaaa gagttgaatg ccaatgctca ttgatgcaac
      541 tgcattgaaa catattttta tcgtttgtgg aaaaacagat cttcgaaaag gaattgatgg
      601 actagcagct ttcgttatag aagaatatga ccttgatgtt tacgatcaag cgcttttctt
      661 attttgcggt acgaagaaag atcgcttcaa ggctctttac taggatcaaa atggatttgt
      721 attaatgtac aaaagatttg aaagtgggta ccttcaatgg ccgcgttcca gaaaagaagt
      781 cttactgact ggcctttcaa tcgaagaaaa atgtacgcta aaagaaacga aaaaaggtgc
      841 gatatattag acgaaaacct tgttaaatca aggattttgt ctgtttttcg ttgtcataaa
      901 atggtataat agatgtaaga aaatcgctag aatggtggtg taaaaatgac aaaaatggaa
      961 tctgaacttc ttgtacaaat caagcaatta acacagttgc ttgaaatcca gcaacaggaa
     1021 aatactcttc ttagagaaca aattgctgaa atgaatcgac gcttgtttgg acgaaaaaaa
     1081 gaaacaccac ccgtagatgg gcaaatcgat ttattagacg actcaacttt taatgaacca
     1141 gagcacactg gacaagaaag ccaggaaccc attacggtat cttcttttaa acgcagaaaa
     1201 cgcaagggac tcaaagcact ttcattagaa ggtctgcctg aagttcctat tcattatgaa
     1261 ttaaaaggtg ccgattgttt gtgtgaagac tgtggtcata ctttacatga tatcggtgcg
     1321 acaaggctta gagaagagcc attgtatgtt cctgcccata tagaaaaaca agtcatctac
     1381 caacatgcct atcattgcag agaatgtgaa gcattaggag aaacaaaaat caaaaaagcg
     1441 cctgttccta aaccgttgat caataatagt ctaggttcct cttcaattgt gacacagagt
     1501 atttgggaaa aatttgttaa aaaggtgccg gcatatcgac aagaaaaaga atggcatgca
     1561 cttggtttcc ctcttgatcg taaaaaaatc actaattggc atatcaaggt aagtgaaatg
     1621 gttttagagc caatctacaa ttgccttcat caagaattaa gcaatcagga ggtcttacac
     1681 gcggatgaga caagctatcg ggtattgaat actcataaag aaaagacctt ttactggtta
     1741 tatgcttcag gtaaaagcga agaaaaacaa gttgtgctct acgagcacgc aaattcgcgt
     1801 tccgttgatg agccaaaaag ttttttagaa tcatttagcg gtttcttgca tactgatggt
     1861 tacgcggcct atggaaagct gccggtaaac aatgtttatt gttgggcgca tgttcgcaga
     1921 aagttttttg aagcattggg taaacaaccg tccccaaaaa gtcatgccaa aataggatta
     1981 gacttctgtg acaagatgtt tcgaatcgaa cggaccttta ataagcttag cgcagaagaa
     2041 aggtttgtag cgaggaagga aacgttgcag cctgtgatga ctgaattttt tcattggtgt
     2101 gagagttttg cttatttgcc aaaatcgcag cttggaaagg cggttcaata cgcagtcaat
     2161 cataaagacg gacttcagat tgtgttgctt gatggacgct tggaactttc aaataaccga
     2221 gcagagcgtg ccatcaaaga gttagtgatc ggtcgaaaaa actggctatt ttctaaaagc
     2281 ttaaaaggcg ctagatcaaa tggaattata ttgagtatta tccagacagc tgttgcgaat
     2341 gggttaaaca tccgcaaata tttaaatcat ctttttacag agattcccaa tctgtcttcg
     2401 atgacacctg aagcgctacg cgcttatttg ccatggaatc agcagataca agaaatctgt
     2461 aaataatata agccacaaat aacctgattt gagtgtatca aatcgggcta tttgtgttta
     2521 ggtaccattc tatgacacgc ttacgctcaa aactactgaa ttat
//
LOCUS       DAFFSV010000204         2539 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFFSV010000204 DAFFSV010000000
VERSION     DAFFSV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.207"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(54..356)
                     /locus_tag="LR110_002856"
                     /pseudo
     CDS             complement(54..356)
                     /locus_tag="LR110_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1070..1360)
                     /locus_tag="LR110_002857"
     CDS             complement(1070..1360)
                     /locus_tag="LR110_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823770.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1568..2259)
                     /locus_tag="LR110_002858"
                     /pseudo
     CDS             complement(1568..2259)
                     /locus_tag="LR110_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          749 a          468 c          351 g          971 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcaacatac
       61 ttgcaaagta atactacaat tcctaacata atcgtagaat ttaataattt ccatagcata
      121 aagtgtttag aaaagaacat taagaatagt tctataataa ttcttgaact ccatgtctta
      181 taatgttcta tcgaagcacc caaatagcca agttcatcta atctacttgc ataaactacg
      241 tcatccgctc tcatagtcgg catatacata ttaaagagta aaaagatacc ccaaatccct
      301 attagtaaca agatcagttc tttgttattc gttatttttc tattatgttt gttcattttt
      361 atactccttc ttatatttcc taatagagta taacatagct ttaagcatta cttaaagata
      421 acttaaaagt tatcttttaa atacttaatt gttaaatacc ttatttgatg caaaagaaat
      481 ctacgatagt caacaaaaaa attttcatta tatccagaaa aatttttctt agttaaggac
      541 ctatggattg cagtaatgca gaattcgtcg aataaactgc caaagacata caatcatatt
      601 gcttttaaaa ttgacgaaca agatatagat tcgtttgtct caaaaatcca aatgttaggt
      661 ttaactgtcg aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat ttacttttac
      721 gactatgata atcacctctt cgagttacat accggtactc tccaggaacg tttaaaatcc
      781 tacaattcca ctacctgaga ctagtaatga ctagtctctt tttatggcct ctgatccccc
      841 cattgagcga atactaatag ggcttcgaat tgatctgttc aatctttaag caatatccct
      901 caaatgagtt tggaaagttt agtgattcat cacaaatact taaatacatg tagttatata
      961 tagattgagg ttcttgtgag cgtatgctcg tgtgggttca aatcccatcg tctgcatcca
     1021 tctttccaaa acaagcggct aggacatttt tgtctcagcc gcttgttttc tagtttattc
     1081 tttcatcttg tcggaccttt tttcttgggt atagactttt ctcatattct accacaattt
     1141 tgggtttccc taacacatga taggatctca caacaaattg ctttctatta ttataataac
     1201 ctgtaacgac taacttcatc ccttcgtcta cttcagctag gaagttcaaa ctatgggaag
     1261 caatcaaaca attgacccca tttaaactga aacggatcaa cggtctggca ctaacttgca
     1321 aagtacggat cttatctact gttccatatt ttttctccat aaccttcacc tcaataccat
     1381 tatacgaaca tacattccct ttgtaaagaa gaaactctgt tttatctttt tgactttttg
     1441 tctattacct ttcttaaaat catattggca cttcctgcga agatgaagga catgtactaa
     1501 aaaaaagagg cagcagtata gattactgtt gcttctttac taatttaggt ttacttgtta
     1561 agaaagatta tttacgcaaa tgttcttcaa caatttgctg agatttatca gctgtatctt
     1621 ccacttcatc ttttgctttt tctaactttt gaatatattg tttcaagctt gagttttcag
     1681 cagttgcttc gtctaatttc ttttgtgtat ctgttaactg atttctacat ttgttttttc
     1741 ttgtgtcagt gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag
     1801 ttttgcttgt gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc
     1861 atttttttgg ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg
     1921 tgcatccttg ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt
     1981 ttggttttct aattcattta cttttgtttg gtattcttgg acatcttcct ttgaatcttg
     2041 caatgtctgt tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc
     2101 tgtcaatgta tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc
     2161 accccacaaa ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa
     2221 tgcaacgaca atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa
     2281 tacttttaca tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca
     2341 tatgtaattt taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt
     2401 aaaaaatctt ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg
     2461 tgaacaaaaa caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact
     2521 gtatttatta caaaaaacg
//
LOCUS       DAFFSV010000205         2403 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFFSV010000205 DAFFSV010000000
VERSION     DAFFSV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.208"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(30..275)
                     /locus_tag="LR110_002859"
     CDS             complement(30..275)
                     /locus_tag="LR110_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823771.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(292..1143)
                     /locus_tag="LR110_002860"
     CDS             complement(292..1143)
                     /locus_tag="LR110_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL4823772.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1309..2313
                     /locus_tag="LR110_002861"
     CDS             1309..2313
                     /locus_tag="LR110_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBL4823773.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          766 a          408 c          480 g          749 t
ORIGIN      
        1 gtatatatac ataatccgtt ccattctttc tattttttcg gtatcaaggc aaaaacaggt
       61 agttcaggtg aataaatgac ttcatgttcg tgccagggtt tgaccattag cgaatgatcg
      121 aaccgttttt tcagttcttc gatgatttga tcatgtgtcc agtttcttgc aggaaaatgt
      181 tggattttct ctgcatcgat caaaaagaca aaatcggaat gaaaagtctg ggtaacttct
      241 tccgagagag aattcaagcg ttcgattgga tccataaaat cacctttcga cttaaaattg
      301 ggtagtatct gggtcttttg ctttgccgac tacaccatct aactgatcga tttgtttcat
      361 ttcttcttct gttaattcaa aatcaaataa ttcagtattt tctttgattc tgcttggtgt
      421 cactgactta ggaagaggaa ggaattcgtg ttgcagcgac caacgcaaag caacttgtgc
      481 aatcgtcttg tcatgtgctt cagcgatttg tttcatttca ggcacatcga agattttacc
      541 agtgcctaat ggactatatg cttcgaggat catatcatgt ttttttgcat actcaactac
      601 agttgtctgt aattcgcctg gtgcaaggaa aacttgattg accatcggca taatttttgc
      661 tgtttttgct agtgtatcta gatggtgggg taagaaattg ctcacaccga ttgctttgat
      721 tttacctgct tcatacagtt cttcaaatgc tcgccatgta tctgcatttg cttgttccca
      781 attatcacga aaagcaacag ggtttggcca gtggatcaaa aacagatcta aataatctgt
      841 ttggagcttc ttcaatgatt cttcaaaaga agacatcaca agttcatagc tgtggtttgc
      901 attccataat tttgttgtca agaatatctc ttctctagga attccacttt ccttaatcgc
      961 ttggccgacg ctttcttcat tcttgtatcc ttgggcagta tcgatatggc gataacctgc
     1021 agccagcgct tctttaacag aggaaacagc gacatcccca tcaggtgttt gccatgtgcc
     1081 gaatcctact accgggattt cgtaaccatt tgatagacga taagtgcttg ttaatgaatt
     1141 catactttca gctccttttt tattcgttca actataagtg tagcgcattt ttcgaaaaaa
     1201 agaatccatt ttgtttctac tcttgaaatt tttgtttaaa agaaaatgat tttgattctg
     1261 gtatttttga ttttttttcc gtaaactaag acagaaggga atgagaaaat ggatatctat
     1321 ttaaaaaaag caattctgca tattattgac agagaaactg gttctccagt cttttcacaa
     1381 aaagagctgg atctgacaaa agaatatatt cgtgattttc ttcaaaagaa gatccaaaaa
     1441 atctcttctg ctcaaacaaa aactggacaa ctagcagaag aaagcatgat cgttgaatca
     1501 cttaaaatcg ctcaagaaaa ttttgtggaa gcaagtgaag caatcgttca gcggtggttc
     1561 gatatctacc aagaaagtga agaagcacct agtgcagatg tatttgttgt cttgtatgaa
     1621 ttagatacag tgatgtactt atctttattg aaagtcaatt atcgtgaggc ttacactcac
     1681 tttgttgaag cagacgaagc agggatcgac aaccgcttga ttttgaatcg agcaattcta
     1741 ggggcgaaaa cacaaaaagc agatgaagca ttcgctgtga atttgactga ttggacctac
     1801 gaattaattg aaaaaaaata tgagttttct ggcaaaaaag agctttattt ttctggaaga
     1861 gtgatcgaaa gtgttcctgc accttcttta gaagaaaatg taaaagtgat ccaaaaagta
     1921 gccaagcatc ttggaaaaaa atttgagaca gaagaatttg atatcatggc tgatttgaaa
     1981 gaagctgtct atgatacgat agaagaaaaa ggtcggttgg atcatgagat gattgcggaa
     2041 aaagtcttca aggaaaacat cacggcaaaa atggctttta aagaagaagt acaagagcaa
     2101 ggatttgtcc cagaagcacc tcctgtaaaa gaagtacaag aaatttctga gaaaaaattt
     2161 ggaaaacaaa aattcaagct atcaaacggg atcgaactga tcgtaccagt agatgtatat
     2221 cgcaacccag atttgattga atttgtgaat aatccggatg gtacgatatc cgtgatgatc
     2281 aaaaatgtgg atgaagtatt gaatcgttta taaaaatgac tagaatatta ggtgttcaag
     2341 taccagtgat cagttaaatg aattgtgtca agagaaggat cggaaaagac gaatataaca
     2401 aaa
//
LOCUS       DAFFSV010000206         2400 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFFSV010000206 DAFFSV010000000
VERSION     DAFFSV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.209"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            206..2068
                     /locus_tag="LR110_002862"
     CDS             206..2068
                     /locus_tag="LR110_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL4823774.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..2400
                     /locus_tag="LR110_002863"
     CDS             2068..2400
                     /locus_tag="LR110_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL4823775.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataattaa
//
LOCUS       DAFFSV010000207         2388 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFFSV010000207 DAFFSV010000000
VERSION     DAFFSV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.210"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            144..494
                     /locus_tag="LR110_002864"
     CDS             144..494
                     /locus_tag="LR110_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4823776.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="LR110_002865"
     CDS             908..1726
                     /locus_tag="LR110_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL4823777.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="LR110_002866"
     CDS             complement(1799..2158)
                     /locus_tag="LR110_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL4823778.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          791 a          346 c          448 g          803 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaata
//
LOCUS       DAFFSV010000208         2380 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFFSV010000208 DAFFSV010000000
VERSION     DAFFSV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.211"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..285
                     /locus_tag="LR110_002867"
     CDS             <1..285
                     /locus_tag="LR110_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823779.1"
                     /translation="LITVKIVDYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLK
                     ESPNEMWFEVTVESSDFKEKKKEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            610..1227
                     /locus_tag="LR110_002868"
     CDS             610..1227
                     /locus_tag="LR110_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4823780.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1268..1567
                     /locus_tag="LR110_002869"
     CDS             1268..1567
                     /locus_tag="LR110_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBL4823781.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1601..1768
                     /locus_tag="LR110_002870"
     CDS             1601..1768
                     /locus_tag="LR110_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL4823782.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1813..2376)
                     /locus_tag="LR110_002871"
     CDS             complement(1813..2376)
                     /locus_tag="LR110_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4823783.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          823 a          371 c          451 g          735 t
ORIGIN      
        1 ttaataactg tcaagatagt agattacttt gcgaatactc ctgaagaaca aatacagaga
       61 aaacttcctg agtttgaaag agtaccacct tatgatccaa gagataattc atttcaagta
      121 aaaaaattaa aagagtctcc aaatgaaatg tggtttgaag ttacagttga atcaagcgac
      181 ttcaaagaaa aaaagaaaga atacaattat attgtaaagg caaaagtatc aaaaagattt
      241 atttttaacc ctaaattaga aatatatagt gttgtaatag aataatagtg ttaaaaaaat
      301 tgtaaaagtt aaagaagtca aattctgatg tgaaaaataa aatataccat ggaagacata
      361 aaagaaaaac tggataacta gtttatttaa tggcagaccg ttaaggcctg ccatttttat
      421 gtattttagc ggaataaata taatcatgcg ggagtgtaaa cttactgttt caaaaagtat
      481 actttttgac atgccttgat tttctttgtt tatgtatggc ataataacca cataatacaa
      541 tttgaaacgt ttctgattta atctgattgt tttgaaacaa ttttagtgaa aaaagggtag
      601 ataaaaacga tgagaaaatt tttttatatc cgggtcagta gttatgatca atcgatcgaa
      661 cgacaactga tttccgcgaa ggaattagga atccctgaag aatatatatt tgtagaaaaa
      721 gccagcggca aagattttaa acgccctgaa tatcaattaa tgaaacggat gtttcgaaaa
      781 ggagatttac tctatctaca atcattagat agactaggca gaaataaaca aatgatttta
      841 gaagaatggc gagaattaac taaggaaaag cagattgata tttctgtgtt agacatgcct
      901 ctgttagata ctgctagtta caagcaacta gatgggttag agacgcttat ctctgactta
      961 attctgcaat tactcagcta tatggccgaa gacgagcgga aacgaattaa tagccggcaa
     1021 agagaaggcg tagaagctgc gaaacaaaag ggtgtaaaat tcggcagaaa gaaaattgct
     1081 ttacctcctg aatttcctgc gatctattct gactggaaag caggaaagat aactgcagtc
     1141 tatgctatgg atcaattggg catgaaacga aatacctttt atcgacgggt gaaagaatat
     1201 gaacatacct tgcaccactt ttcttgatag atataacagc tattagacgg gaagggagag
     1261 tttgaaaatg gacttaacga atcaaagggt cgtttttact ggaacgctgc agcaaatgac
     1321 acgaacccaa gccatcggtt tggttcactc tttaaacggc cattgtcaat caagtgttac
     1381 ttcaaaaaca aatttcctgg tttgtggtca gtattctaag tcgttgtttg atgattctct
     1441 ctatacaaaa aaggaacaaa ccgcgcggga cttgattgcg ttaggtcatg agattaccat
     1501 tttatcagaa gtcgaatttt atgcgttaat tagtcaacag ttgaaagaat ggcaacggta
     1561 tttataatcg ttagtaaaat ttacaaggag gaaaattttt atgaagcgaa aatcttatga
     1621 tcgttggggc attgaaataa ctagttttaa tcgaaatatt gcttttcacg tggagtgtga
     1681 ctgtggggaa ttagcaaaaa gtgtttatca aaaaaattac ttttactgtc ctaaatgcca
     1741 tcgccgatac ggggtacatg gaacatagaa acgaaatagt gcggtagaaa agcgaatgaa
     1801 tgtagtatat ttctacaaat cattcgcttt ctttatttta taaatagttg gtcgagagat
     1861 cccgttttct tttgcaatct ctgcaactgg aatgccttgc tttaacatgt ccaccacggt
     1921 tttaaaaact aagcgttttt gcggatctgt tgagttaata gaatactcga ttggtcgtcc
     1981 cttatatcgg ccggcttttt gagccaataa tactccttgc cgctgacgtt cacgaatttt
     2041 ctcacgctcg ttttgtgcaa tataacttag cagtgataaa aacatatcga acatagcggt
     2101 atccaggagt tcatttccag tattaaaatc taaaaactgt gcatctaata tttttaacgc
     2161 tacttttttc tgtttcataa aagcaactgt attttttata tcttcataat ctcttcctaa
     2221 acgatctagt gatgtaacga tgacgcaatc accttcacgg atataattta gtaatttttg
     2281 aaactcagga cgctcagtat ctttaccact ggctttttct tggaataatt tatcaatgtt
     2341 aagtccttcg gttctgttgc gaagtttgaa aatcaaacgc
//
LOCUS       DAFFSV010000209         2350 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFFSV010000209 DAFFSV010000000
VERSION     DAFFSV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.212"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1341
                     /locus_tag="LR110_002872"
     CDS             <1..1341
                     /locus_tag="LR110_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823784.1"
                     /translation="KKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1328..>2350
                     /locus_tag="LR110_002873"
     CDS             1328..>2350
                     /locus_tag="LR110_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823785.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWR"
BASE COUNT          779 a          360 c          407 g          804 t
ORIGIN      
        1 aaaaagatag atgctgcttt tttatttagt tgtttatata cttttgcaac ttatcgaaca
       61 gttgatatct attatcgatc cgcaattgca gaagcaatcg ctcttacgat tatcccagct
      121 gttttatatt atgcttatgg attgatattc aatagagaaa aaaaagtcat cgctttatct
      181 gtcagtataa gtctacttgt gtatactcat gtcttaacta cactattgtg tagtgcattg
      241 attgcttgta tgattatagt tcgtttgttt ttaagcaagc aaataaaaaa cgatataaaa
      301 tttgtggtcg tgaatttagt aaaggctgtt tttttgactt gtgcattaac tagctatttt
      361 tggtatccta tgttgcagca gatgaattat caagaaatac acagtccctt caaaacagat
      421 ttgcagctag aagcactttc tatttcagat agtatcattc aagcgttaaa taacgatatt
      481 acaacataca ctatggggtt attaggattg gttgcgttga tttttccttt attcatactg
      541 aaagaaatgg agaaaaaaga aaaaatcatc tatgttggcg tagtttctac atgggtatgc
      601 gtaactaatt tatttccatg gtttttgttt caaaaaacac cgataagctt gctccagttt
      661 ccttggcgta tcttaggtat ccaaatcttt ttttcttcaa tgatcataac gcttgttttt
      721 atgaaaaaga ctatgaataa aaaatattca tggttaatca ttggcgctac tgtggtgttt
      781 ctactcgtca cgtctatagc agcaaaagaa aattacgcaa aagttatcca atcaaaacac
      841 ggtcatattg ttatcaatga agaaacactc ccacgttata caacatcaga tatgggagga
      901 ttatacgatt acgccccaac agaagcgata aaagaaaggc ctcacttaga aaaacatgaa
      961 gtaaaagtag gagaatcgtg ggaaaaaggg aagtataaag ctgcagataa taacattact
     1021 tacacagtgg caagcaacaa aaagcaaaaa gtgacagtac cagtatatgc ttatttaggt
     1081 acacaggtaa aagtaaacgg taaagtcgta aatgactctt ataaaaaaca tggcttagtg
     1141 aatgtggatg taaatgctgg agaaaataag atagaaatta caacaaaata tactccaact
     1201 gctctcatgg ctttatggat cagtattctg acttattgtt tagtggtgta ttcaagtttt
     1261 aaacgatttg cacctaataa gttgaaagat tctcgatatg tgactaaaaa ggagataaac
     1321 aagaagaatg aaaataaata aaaaagtagt aatattgagt ggattaatta tagttagtag
     1381 tatatattct ttcatctttt tcaaaaatac acagagtatt tataccactg gagatctttc
     1441 ttttcactta tctcgaatca aggggttatc gactattttt accagtccga ttaattatga
     1501 gacattcaat tatactggat atggtgtaaa ttatttttat ccctttctga ctttttttcc
     1561 agctgtaatg ttatattgga tgacaaaaaa tttgattgta agctatatta tttacgtttg
     1621 gttattgaac ttgtgtacaa cactcgtagc ttatcattat ggcgagcggt ttttaaaaca
     1681 aaaaaaagcg gcatttttat ttagttgtct ctatattttc tctgcttatc ggacagtaga
     1741 catctactat cgttccgcaa ttgccgaagc tatcgcaata acacttgtta tccctgtatt
     1801 attttatgct tatcaaatca tctctggaaa agaagaaaag tatccgtctg taaagctagc
     1861 attgagtatg agtttgctag tgtacagcca tgtactatca acattaatga gtacagcatt
     1921 aattatcatt tttattttta ttcgtttggt ttctaaaggt ttcaagaatg cagattttat
     1981 tgctatattt aaaaaattat ttagtgcggc aggaatgaca ttggttctga cgagtgcttt
     2041 ttggtatccg atgtttgaac aaatgctcta ccaaaaaatc aacaaaccta gcgtgacaaa
     2101 tttatatgct catgcatcta atgtgttcga tagtctgact gaagctatga acaacgactt
     2161 aactacttac tcaatgggac tagttggtct gttatcttta tgtattcccc tgattttatt
     2221 taaaaaatta acaaacatag aaaaaaagat ttattatggt acgtgtttga cttggctagc
     2281 tacaactagt ttggtgccat ggtatcttct gcaaaatacg ccagccaaat tgttacaatt
     2341 tccttggcgg
//
LOCUS       DAFFSV010000210         2308 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFFSV010000210 DAFFSV010000000
VERSION     DAFFSV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.213"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..418)
                     /locus_tag="LR110_002874"
     CDS             complement(<1..418)
                     /locus_tag="LR110_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823786.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPT"
     gene            complement(446..1084)
                     /locus_tag="LR110_002875"
     CDS             complement(446..1084)
                     /locus_tag="LR110_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL4823787.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1088..1858)
                     /locus_tag="LR110_002876"
     CDS             complement(1088..1858)
                     /locus_tag="LR110_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBL4823788.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT          735 a          439 c          354 g          780 t
ORIGIN      
        1 cggttggatt gtaccaacat gagaatgtgt cagttctatt tcctgtcgca cttgcatagg
       61 cccatactcc ttttgttgca tttgctgatc gaatagagtt tccaactttc gcttttgcac
      121 cgtgacgatt atagacacga ctatagtggt tagaatgacc tgtttttctc gtacaagttg
      181 tatcactata ataaacccat cttccagagc tatgattaat tgctgttcta gaaatttccc
      241 ggtctactgg taaatttgac ttaataatat tacctttagc atcaaaatga atctctcctg
      301 gctttaaaga tttgtaatag atttcaccgt gaactgaatt tgcttccact tcagatgaca
      361 tagcgtttcc tccaacaaaa cctatcccta gtgcacaaat aataactgct tttttcataa
      421 aacgacatcc tttccttttt gatttttaaa gcatgatctt cttgtttcct attttggtca
      481 gatatggatc atgcgtgact acgataacta acgtaccttg ggaagcttgt tgcgataaga
      541 tttcaactac tttatcctta tttcttttat ccaatgaccc agtcggttca tcggctaaaa
      601 tgattttgct attcttcaat aagactctcg cacaggcgat tctttgcttc tcaccaccag
      661 ataatgtgta gataggcgta cttagcttct tgtttatatt tacttttttc aatgcctcat
      721 actttgctgt tcgcttatct gaaacattta aattcgaaaa cttcaatcca atatctaaat
      781 taaaatcgat tgtttcatat tcaatcaacc caaaatcttg aaaaagaaaa gatatggtat
      841 ttctttttat caagcgcttc tctctcccag ttaattgttt cgtctcttga tcgaacaaat
      901 agaattgtcc tgaactgaac tgatcattca atcctaatat attcaataac gtacttttac
      961 ctgctccact ttctccaaca acagtcacaa gatccccagc atccgcagtg aaacgatcca
     1021 aatgaagaat cgttttgttt cgaatgattt ttttcgcacc ttttatcact atcaacggct
     1081 ccaattgtta acctccattc atcagcaata actgactttt cttttctgta gacaaaataa
     1141 aaacaactga aagaataaaa tcaatgaagc taaagaaaaa aatattaaaa agatatttag
     1201 gctctctttc tataagaaaa aggaagatta cacttgtcgt tccccatttg atgaggttca
     1261 tcatgaagaa atctttatac gtttcaaaga gcgaataacc acttgaacga aagatagcga
     1321 ttttccgaca attgtagaca aaatatatgc ataacatgac atagctcaac aagaagaaag
     1381 aaaagaaagt gagtaaaaat atctgtatca agtaatgaaa tgatctgctt aacactttga
     1441 cagctatctc ttcagtctga gtatacggaa atgataactg caagttatca cgtaactgac
     1501 tttcttcaat cagctcaaat aacctttctt tcgacaaaga gcctatcttg atctttaacg
     1561 gtggatagat ttctccgctg agaatatttc tttccaaata catactgttt ttcaatgtca
     1621 gtatttcaac aaccggataa tgttcgatcc aggggataga aggaataaat gtataaatgg
     1681 gttgattgtt ttttataaat agaaatttcg gttctttttc actgatttct tgataatatt
     1741 ctgttatttg cgccaggtca ttttttaaat ctgtatgtct atatcgttca gggatcaata
     1801 taacagggtg cgtttctttt tcggttattt ggatcaacct tccagagaca tcaatcaaag
     1861 gaaatttctt aagataattc agatttattt ttatccctct gccaaactgt tcattttctt
     1921 cattcaagta atctgataca ttgactaata tacttccgtt tttattcaaa gtttcatata
     1981 attcctgatc tgctgctgat gcaaaacgat tggaataaat aaaatccacg tggtttttcc
     2041 cgatggttac tggataaaat acatgatagt cctcaccagc gatttctttt ggtggttcat
     2101 gggaaacagc taactgtgaa gaagaaaaaa cgatagttgc taattcgaca ttggcaacgg
     2161 tcaaaagaag agaaaacaac aatccaaatg taaaaagaat gtattggata tagttgaacg
     2221 cttctcttaa cgaagcgatt ttttcaacta ataaaagaga aatcaaagag ataccatata
     2281 ttccgctgaa caagaaaacg tacatctg
//
LOCUS       DAFFSV010000211         2302 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFFSV010000211 DAFFSV010000000
VERSION     DAFFSV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.214"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(545..889)
                     /locus_tag="LR110_002877"
     CDS             complement(545..889)
                     /locus_tag="LR110_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823789.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(986..1231)
                     /locus_tag="LR110_002878"
     CDS             complement(986..1231)
                     /locus_tag="LR110_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4823790.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1301..1555)
                     /locus_tag="LR110_002879"
     CDS             complement(1301..1555)
                     /locus_tag="LR110_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823791.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1719..2042)
                     /locus_tag="LR110_002880"
     CDS             complement(1719..2042)
                     /locus_tag="LR110_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823792.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2229..2302
                     /locus_tag="LR110_002881"
     tRNA            2229..2302
                     /locus_tag="LR110_002881"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2263..2265,aa:Ile,seq:cat)
BASE COUNT          861 a          386 c          405 g          650 t
ORIGIN      
        1 aaaaaaagaa agcgttatta ttttagtcta ttttcagggg gggaacctta tgataaaaaa
       61 actattttta ctatcaatat taacgctagc aatggcaggt tgttctgact ctggcttagc
      121 aactgaactg atcacaaaat caactgaagc cacgacagag gagttaaaat gtggaaacga
      181 tcacagtgac tacagaggct taaatcgacg atgcatggga atattaggca gaagcaattg
      241 taagcatcga tgtagtaatc gaagaaggtg tagaagaagc aacagaaaaa tcaaaagaaa
      301 gatcaaaaga agcaatctgt gctgtgcatc gtatacagac gtcgaagatg cggtactttt
      361 aaaccacgtc aggagatgtt catttatgtc tagggaagtt ggcgatgaac caaaactaga
      421 gtacgctttt agttttgcac gtgaggatga agataaccct aaaacttacg acattttagc
      481 ttgatctaag atggttaaag ctttttaatg aagtaagagg agatagaagg cttttctagt
      541 tatattaggt aagataaatt cctttttgct ccaaatttgc taaacattct gctagataaa
      601 cttcatcgtg ttccggataa atcggcacgt tgcgctctac agtaggtaaa tcaaaaaatg
      661 cttcgcatga tttgccacga tacataggat ttttccactc tggtgctggg ctgtaaccac
      721 gtttttccat ttcatccatg acaagctgat gatattggaa taatctataa ggagaatagt
      781 gaaaaacata atccacagta gcatgtttct ttccccagcc atttcctctt aatgcacagc
      841 attcccgatg ttgtcctaaa agctgttgac gaggaagctt tgaaatcaat ttttcatgcc
      901 ataatcacat agaaatgtct cctttatata tagattgtta accttagacg ttaaatgaaa
      961 aaaccagcac caaggctgga aaaaatcact tcagtttgct tgctgtttct gagcgaccaa
     1021 ttaggtaatc aacggaaata tcgtaaaagt cagcgatata gattaattca atgatgcttg
     1081 gaatttcttc tccttcttcg tacttacgat atttgaataa aggaatactt aacgcgctag
     1141 aaacagctac ttgagataaa gatcggccag ttcttaagaa attcagacga caacgtaaag
     1201 tatccagtaa aatagcttct tcgatactca tttaatcacc tttcattata gtagaatagt
     1261 tataatgact atagaaagct acacatgcaa tcaatagtac tcaatgtttt ttatttaaac
     1321 gtatcaaata aagaatgacg atcaataaag acaaaaccag tcctgataat tcttctataa
     1381 aaacactatc gggttcaaag tagttgtaat taaaaacttt ccacataaag tagatagccg
     1441 gaagagtcaa aatagctaaa taaaaccgtt ctttgttcca attcataaga taaacaatac
     1501 ttgcgaccag tgcgaccgca ggcacccaaa acaaaactct caccaacgta atcatgtaac
     1561 ggactcctca acgattcata taataaaaga taacatgtta ttgcatacat cacaaatgaa
     1621 atgctaggaa aataaaagaa gaatagatca atgaaaaatg aaaaagcaga acagttttct
     1681 gctgataagc aggaactgtt ccgctgaatg tgtcttatct atcgagaaaa aatcaatgga
     1741 ttgatatcta agtaattcaa tacatataaa aagaggacga tcgcattcac taaaaaggaa
     1801 agaatgatga tcccatagcg aatggaaaca tatttaacaa atacttttgt ttgaatacgg
     1861 gcatatcttt ttcctttata gctgagcatc atgacaagaa gcaaaagggt aatcacacct
     1921 tgaaaaatca ttccaataaa cagaattcct gaggtttgat aatttaattg gagaaaataa
     1981 gctgctgccg caactaaatc gaataaaatc gcaataaatg gcaaaataat cactcctaac
     2041 attgagaata aataaaagaa taacattttt tctaaaataa gcaaataaaa attaaagtaa
     2101 caaaatagtt atgacacaag taaaagaaat aagaataaca atattatttt acttatattt
     2161 tgaaacttgt gagagtttgt cattcgtgct atagttgaaa agtaacaaaa ataaatttta
     2221 tttatgatgt acctttagct cagttggtta gagcagacgg ctcataaccg tccggtcgta
     2281 ggttcgagtc ctacaaggta ca
//
LOCUS       DAFFSV010000212         2262 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFFSV010000212 DAFFSV010000000
VERSION     DAFFSV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.215"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..445
                     /locus_tag="LR110_002882"
     CDS             <1..445
                     /locus_tag="LR110_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823793.1"
                     /translation="VKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIHEDKVK
                     DHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLPYQVAE
                     DQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            468..697
                     /locus_tag="LR110_002883"
                     /pseudo
     CDS             468..697
                     /locus_tag="LR110_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            718..1791
                     /locus_tag="LR110_002884"
     CDS             718..1791
                     /locus_tag="LR110_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HBL4823794.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            1835..2104
                     /locus_tag="LR110_002885"
     CDS             1835..2104
                     /locus_tag="LR110_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823795.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
BASE COUNT          853 a          310 c          442 g          657 t
ORIGIN      
        1 agtgaagtta gcacatgtac cagaagaata tattgctatg cgattaacta ttattcctga
       61 aaaaattgat ccaaaggtgg atatgcaaga accgcaagat ctgatttttt atattcacga
      121 agataaggtc aaagatcacg tgaaagacga agattatgaa caacatgcga tcaattacaa
      181 agtgaaagga tacaagaaag aacaaaaagc agcacaaaaa caaattgaat ccttccaagc
      241 aaccattgat ttgaatgaag aattagtgaa gaaactaaaa gatcaattgc cttatcaagt
      301 cgcagaagat caggaaaata ctgaaaataa gattaatagt taccagcaag aaattgaaac
      361 atcgaaaacg caaatgaaag atcaagaaga gagcattcaa aaacttcaag aaaaaattga
      421 tcgtatgaca aaagaaaata tttagaagaa taggtggagt aaaagaaatg aagcgagcga
      481 acttggtcgg caaagatact ttcgctgagc agaaagtatt gattaaagcc tatctcaaag
      541 ttgtaaaagc taaagaaata tgtcaagaag tgaattatct aacacaacaa tatctgagtt
      601 gaagaatggt agaaagagtt tagatacctt aaaattcaga tcatttgaag ctctatatat
      661 agctgctaaa aaatatttta cagctgataa taaataaaaa attaaggtgg gttctatgtg
      721 aaaaaaacgt atcaaaagtt tttatggatt agctatatcg tcctatttat cgttgggttt
      781 ctcgtcagtg tgtccgtgat cgtcggaacg ctttttcttt tgttctctga cgaagatctg
      841 gtacttaagg ggttaatgag tacgttatcg tgtgtcatgt tactttattt atgtatacag
      901 atcaacgaaa ttatgccccc tcattggcaa gaaaagaaga aagaatttaa ggaagaaatc
      961 gaaataaaat ccaatgactg gattgaatac acatggcttc ctctgaaaag aaatagcaaa
     1021 cgaaaggaat tttgggcatt gcaattatgg ccaataggaa aactttttat ttatatggct
     1081 tcacttatgg ttactttaat cggttatttg gtggtggtct atggggtggc ctattacttg
     1141 ttaagtgagc caaaacagat cgcggcgtca aatattttca cggcagtaat gacattaatg
     1201 tgtgtcatgt gcttttctat ttttagttac ttactcgtcc aatttatcag gcaaaattta
     1261 acaatgagta aacttttcaa aagtttattg gacttactta tcttaggcag tatgttaacg
     1321 actctaacag atcggttcga acaatctgaa aaaacggtag aggggataaa gaacttctta
     1381 acgacgattg atcaatttat aaatggatat atgtatttgt ttgaagtagg ctttattctc
     1441 actttgtttg gttgtgtatt tataaaactc attaaaagat caatcgaaaa agaaatggtt
     1501 attgatctaa ttccgacgct gagcggctat gagtattacg ttgattttgg aaagtatgag
     1561 gagttgatcc atacggatat cgttcacacg agtatccatg aagtaaccgt taagggcatc
     1621 agatctactt taaaagatgg acaggagacg aaaacaatcg accgaagaaa agaggtctac
     1681 aatttttata aagttgaagc aagtctacga tatggcattg ggaaattgag gttagatggg
     1741 agtaatattc aacaaaacaa accatatgtt ttaggaaaag tggtgattta gaacttccta
     1801 tgaaagatga ataaataagg agaatctttt gaaaatgatt cttctttaca gaattgtgct
     1861 tatagttggc acggagttaa aaatcttaaa gaaggagcag ttattcaaag tgaaagaaca
     1921 tcataatcag ttactatgta ttaaaacggg tacgatgaac attcaatacc gaacagaata
     1981 tttagaatta acgaatcaaa ctgatttttt gtcgattcga gagcaacata agcaagaaaa
     2041 attaatggct acttatagcc aattgattga tgagtgggaa caattcaagg aatctaaaac
     2101 ataaaattcc atttaaacac cttatttttt aaagagcata aggaaacgtg tacccacttt
     2161 caaaataaag tgggtagata ggctgtgcct atcacgtttc tgaaaaaaat aaatgacccc
     2221 aaaattttca atttatgtgt aggtttatgg gtagcaaaaa ta
//
LOCUS       DAFFSV010000213         2235 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFFSV010000213 DAFFSV010000000
VERSION     DAFFSV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.216"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            59..1105
                     /locus_tag="LR110_002886"
     CDS             59..1105
                     /locus_tag="LR110_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBL4823796.1"
                     /translation="MTRPNHIESSKKEAPKTEGQTKNFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1454..2125
                     /locus_tag="LR110_002887"
     CDS             1454..2125
                     /locus_tag="LR110_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBL4823797.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
BASE COUNT          832 a          300 c          449 g          654 t
ORIGIN      
        1 ttcaagtgcc gaatcttcaa caacttcaag tagtacaagt gaaataactt cttcgaaagt
       61 gacaagacca aaccatatag agtcttcaaa aaaggaagcg ccaaaaacgg aaggacaaac
      121 aaaaaatttt tcgacaccac agcaaataga atcaacagaa tctttccaat cacaaccaat
      181 actatctggt aatgaagaaa ttcattttga caaaaatgaa tctgtagaaa attttattat
      241 gaaaattggt gagtctgcga gaaaaattgg tcagaaatat gatttatatg cttctgttat
      301 gattgcacaa gcgatcttag aatctgccag tggtcaaagc cagttagctc aggcaccgaa
      361 ttacaattta tttgggataa aaggaactta taatggaaac tttgtcatta tggtaacaaa
      421 cgaagatttg ggtaatggaa cgctttatac gacccaatca aaatttagag tgtatgaaaa
      481 ctatgaggaa agttttgaag attatgccaa attattaact aagggaatta gtggaaataa
      541 agatttttat gcaggagcat taaaagctaa tagtaaaaca tatcgagaag caactaaatt
      601 ccttacagga cggtatgcaa cagatacaca atactatttg aagctaaatg aattaattaa
      661 aacctatgat ttaaccaact atgataaaga agttaaaggt ccacaaatga gtaagactgg
      721 ataccaagtg cctattcata attattcaat aaccagtttg tttggaaatc gtggaaatga
      781 atttcaccga ggacttgata tggcagctac acagggtgag ccgattcatg cagttaaagc
      841 tggtaaggtt gttaaagcag agtttcacta ctcttgggga aattacgtgg tgatacaaca
      901 cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa tacctagtaa aaactggaga
      961 taatgttttg caaggacaaa taattggcta tgtaggaagt actgggaata gtactggccc
     1021 acaccttcat ttggaaattt gcaaagatag tagtctttct cagagcatgt tagttgatcc
     1081 gaaaacctta ttattcggag actaaaaaag gtaatcaatc tgacaaagta acaagaaaat
     1141 ggttggattc ttagaccttt gatcaggata ttgagactgg aattgatatc tcgattacga
     1201 tgagtttgac aaataaaaca agtccattat cgaatatcga gtgtattatt gccgtcttta
     1261 tgctcatatt ttgagcaggt tttactagat gaaaacaatt tatctatctt gaggatttcg
     1321 tgtacgtatc gttcagtctt gtattataaa ttagtcataa aatcagacta agaaaaattg
     1381 aaaatatttt tttctgattt attaaaagtt aattgaaatt atatggtact tctttcttgg
     1441 gagggtaggt gcagtgaagg aaagatatgg tcaccttact gttgttgagg agattactca
     1501 taatggatat aaaaaatatc gttacctatg tgactgtggg aatgaaacga ttgtatatcg
     1561 atcaaactta ctatcaggta gaaccaaaag ttgtggatgt ggaaattata aaaataggca
     1621 taaatataaa aatttgacaa ataaaacttt tggtaagtta gttgcaatca agccaactaa
     1681 actgcgaagt gaaggaactg ttgtttggga atgtcaatgt ctgtgtggga gaaaaaagtt
     1741 agtatcggca aagcgacttc taaaaggtga tgtaaaaagc tgtggatgtt tgaaatcaaa
     1801 aaagcatcac attgaaaata aaaaattcgg attacttact gcaaagtatc ctttggacaa
     1861 taactgttca tggaaaacaa aatgggtttg tcaatgtgag tgtggcagta tgtgtgagat
     1921 aagttatagt aacttatttt ttggacatac tcaaagttgt ggatgtttaa aaaggcaaga
     1981 aacaaggaca tgtgttgaag ggacagtaat agagtgtcta acgtcaaaat taccgaaaaa
     2041 taatacaagt ggtgttaagg gagtttatcg ttctaaagag aaatggattg cgtatataac
     2101 tttaaaaaaa agaggtacta tttaggatcg tatgattcta ttaagagtgc agctcaagtt
     2161 agaaaagatg ctgaaaataa attgtttcaa cctttgattg acaaatatcg aggtaatcat
     2221 taattattgt ggata
//
LOCUS       DAFFSV010000214         2212 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFFSV010000214 DAFFSV010000000
VERSION     DAFFSV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.217"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            50..1663
                     /locus_tag="LR110_002888"
     CDS             50..1663
                     /locus_tag="LR110_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL4823798.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTGDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          690 a          392 c          442 g          688 t
ORIGIN      
        1 gttttttacc ataacatagt acgatcaacg tagaaaagga gatgttttta tgaaagttgt
       61 tgtcattggc ggtgtagctg gcggtccttc atttgccact cgtttccgtc gattgaatga
      121 agcgcacgaa atcattatct atgaacgcgg agaaaatatt tcttatgcaa gttgtgcttt
      181 gccttattat ttaggaggtg tgatcactga ccgtgactcg ctgatcgaac gtacaccaga
      241 aatattgaaa acaaaaaaca acatcgatgt ctttactaaa cacgaagtaa cagcaatcga
      301 tccttctact aagcgattaa cagttaaaga cctatccaca aatgaagaaa taaaaacaga
      361 ttacgataag ttgatcatct cttctggtgc tagaccagat tatccggata ttcccggagt
      421 ttttgaagca gaaaacggct ttgtcctccg tagtgtgacg gatgcggatc gaatcaaatc
      481 gttccttgaa gaaaaaaatc cacaacatgt cgtcattctt ggtgcaggtg ttatgggtct
      541 ggaattagct gagaatttca agcatcgcgg cttagacgtg actttaatcg atcaattgcc
      601 acaagtcgct ttcccttatg atccagaaat tgctaattta gtttatgaca aattgctgaa
      661 agaaggatta gccgttcatt tagaaacaag agttactgag atccgtgata aaggtcgaga
      721 aatagtatta tcagatggct ccgtcctttc tgctgatatg ctaatttttg ctgttggtgt
      781 ttctccgaag aatgaagtgg tgaaagcaac cggcatacaa ttatcagata caggtcagat
      841 cattgttgat gaccagttac aaaccaatct gccggacatc tatgcgattg gcgatattat
      901 cgaaacgact agtgtagtga ctggtcagcc gatccaaagt atgctttcca gtgcggccaa
      961 tcgtcaagga catatgctgg cagatatttt aaatggtacg cctatgcgct atcgcggata
     1021 tattggtgca ggtgtcgcaa aaatctttga tcatacagca agttatgcgg gaatgacaga
     1081 acatgaatta aaagcatcag gcataacaaa ttataaaact gtttttatca ctccctttga
     1141 ccatgcctat ttctatccag aagctacaag attaaatcta aagctgattt ttgatgcaga
     1201 tagcggtcgt attttaggtg gacaagcatt tggagaaaaa ggtgtcgata aacggatggg
     1261 agaactttct gtagcaatca ccggaaactt gacagtcttt gatttgcctg atttggagtt
     1321 gccttactct ccaccgtatt ctactaccgg tgacccgttg aatatagctg gttatgtcgc
     1381 aatcaatcaa atgacgaata tcgtagaaac gatcaaagca agtgatatac ccgaaaacga
     1441 tttgaaagaa gcgttctttt tagatatacg tgaacctaat aaagcacctt ctggcagtat
     1501 ctcggcaaca aaaaatattc caatgaatga gttacgagat cgaatcaatg aaatcccaaa
     1561 agataaaaaa gtttacatta ctttcagaag aggattgaat acttatactt ctgcccgaat
     1621 cttggcaggt ttgggtatca aagcgatttt gattgaagaa taaaaaaacg accgcggtgg
     1681 gtagccgcgg tctaggagtt atttttactt gaggagtaaa aatgaaaaag tatatagggt
     1741 tgttggttgg tatgtatata gaatagaaga taaatgtgaa gaaattgtgc attatttctt
     1801 cacatttatt aggttatagt ttaagaaagt ttaaacattg cgcaatttaa ttattttagc
     1861 tgtttctttt cataaaaaaa catgttgtga ctctgagaca aatcatagtc catagtgatg
     1921 tctctagagt aactacgcct aatattacga ggcatcatat taatctatca gcactagtac
     1981 ctaaactagc atccctttcg aatatttttt cttatataat aaagtttgct ttcactcctc
     2041 taatctcacc aaatcgactg gcttgcgttg tgtgatacta ggaactgatt ggtttagaac
     2101 gacttttcta ttgtaatcat atttttcgcc atcctcgttg tcttctaatc cttcaatgaa
     2161 tgatccctaa tgtgtgtatt aaataattta atttttttgt tcctatttat tt
//
LOCUS       DAFFSV010000215         2131 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFFSV010000215 DAFFSV010000000
VERSION     DAFFSV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.218"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..195)
                     /locus_tag="LR110_002889"
     CDS             complement(<1..195)
                     /locus_tag="LR110_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4823799.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
     gene            869..1681
                     /locus_tag="LR110_002890"
     CDS             869..1681
                     /locus_tag="LR110_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823800.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          714 a          302 c          347 g          768 t
ORIGIN      
        1 ataatagcca tttcgttgac tttgtcggtt ttctgttcgt tcatattctt ttgcttgaat
       61 atattctgtt cgttgatttt ccattagttg attaaatacc gttgttaaaa tatttttaga
      121 aacgtcatcc tttacagaat attcaataat gctttgaatc tcttcgcttt tcagtgtaaa
      181 atgtacttgg gtcatgtaaa agtcctcctg ggtatgtttt tgtcgttaaa aacattgtac
      241 cgtaaaagga ctgttacatg gcctttttac ttttacacaa ttataaggac tttatcgaaa
      301 aaaagcagtt atttgttaaa atcattgata ttattaataa taagtatttt ttgaaagggg
      361 aaatataaaa ggtttttgaa aataaataaa aaatagtatt acttgttcta ttgctagtta
      421 ttgctagtct ttattctttt ttgtttttta gggatgtaca aaagatctca accacaggag
      481 acattgcttt tcacttttct agaataaaat gcttgtcctc agtattttct ggtccaataa
      541 attttaccac atttaatagc tatggcggag gcgtgaattt ttttatccat atctaacatc
      601 tttccagccg taatatttta ctggacaaca aacaatttga ttctaagtta tatacttact
      661 atacgttggg atactgaata tctgtacaat tctagtgagc tatcaatatg gacaaaagaa
      721 tagatgcagc cttcttattc agctgtttgt atagctttta tggattgata tatattagcg
      781 tactgcaatt tcagaatcaa ttgcgttgac gattgtacca gttgtcttgt attacacata
      841 tactcttctt tatgaaagaa aaaactgcgt gattctttta gctgtcagta tgagtttgct
      901 catctatacc catattctct ccacctttat gagtgtgatt gttattggct tcttgtttat
      961 cggctctctt ttctctaagc ggaaagagag aaaagaaatg tcgaatctga tatcaaatat
     1021 gataaaagct gttggttgga ccattctttt aacagcttac gtttggtatc cgatgttaca
     1081 acaagtccta catcaaaaaa ttaatcgtcc ttttcgcaca aaacttcaag aaagggcatt
     1141 aaacatttct gatagtttaa tcggtgcagt aaataatgat ttagttacat ttacaatggg
     1201 aacaattggt gtggtttcat ttattcttcc acttttcttt aaaaaaaaat ttgccaataa
     1261 agaaaagatt attaattttt gtgcagtgac cacttggttc ttatcaacga atctttttcc
     1321 atgggaattt ttacaaaaaa cacctattca aatcattcag tttccttgga gaatcttggg
     1381 ttttcaagtg ctttttggtt cattgatctt aattatagtg tttttaaagt ggaaaacaag
     1441 caacaaaaaa agtatgtgta gccttgtagg aatagtcctt cttattttta cagtgactgt
     1501 agcaacagaa gcgaattatt cgcagaaaat tcaatcgtat aaaggtcgtt taattatgac
     1561 caaaaaagat gttacacttt atacaacctc gagaactgga ggtctttatg actatgcacc
     1621 attagatgca ttgaaatata aagatcattt aaagaaacat gaagtaaaag tctatgattg
     1681 atgggagaat gcatctcata aagcacataa ttctaaaata atctatacag taggagatgc
     1741 gaatgagcaa aatatcacgt tacctgtatt taattactgg gaacagttat gaaagtaaat
     1801 gggaaaaaga gcactataga aaatgacaat ggtttagtgt ctattaaagg tacgggaaaa
     1861 agaactatag ttgaggtttc atctacgtat ccaactacga tgattctatc tttattaatt
     1921 agttcaataa cttttatatt cttatttaca cgttatttaa taagatgctg cggttaaatg
     1981 aaaatagatt agatagatat tataaatatg ttgattctat tttcatagca atggagaaaa
     2041 tacattagca atatataaaa gaaggtatca agagtcacgg tgaatttatt ccgaaaagtg
     2101 aaaagaacaa atataaaatt taataaaaat a
//
LOCUS       DAFFSV010000216         2102 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFFSV010000216 DAFFSV010000000
VERSION     DAFFSV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.219"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            169..1539
                     /locus_tag="LR110_002891"
     CDS             169..1539
                     /locus_tag="LR110_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HBL4823801.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="LR110_002892"
     CDS             1560..1844
                     /locus_tag="LR110_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823802.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          730 a          308 c          444 g          620 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 ca
//
LOCUS       DAFFSV010000217         2092 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFFSV010000217 DAFFSV010000000
VERSION     DAFFSV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.220"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>2092
                     /gene="acm"
                     /locus_tag="LR110_002893"
     CDS             <1..>2092
                     /gene="acm"
                     /locus_tag="LR110_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBL4823803.1"
                     /translation="ILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDG
                     GNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKD
                     SAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPAS
                     GSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEI
                     NQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKT
                     IIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYI
                     NDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNW
                     IDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFR
                     ASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGY
                     QKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEK
                     DDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHI
                     TPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILP
                     KTSSKKSSFAVIIG"
BASE COUNT          818 a          343 c          405 g          526 t
ORIGIN      
        1 ttattttatc aatgttattt attattacta atataacatc actgattcct gttcatgtct
       61 atgcggatgc aggcagagat atcagcagta atgtcacttc gttgaccgtg gacccaacga
      121 atattacaga tggaggaaac ataaaagtaa aattctcgtt tgacgaaaaa aaacaaaata
      181 tccaaccagg tgattatctt tggatcaatt ggccaagtga aggaaatatt cgcggtgaag
      241 gattccaaaa agaaatccca ttaatgattg aaaataaaaa tgttggaact ttaacggtca
      301 gaaaagattc tgcgcaggtt gtgtttaatg aaaatattaa aaacctagac tcagtagaag
      361 gttgggggca atttgagata caggccagaa acgtaaccga taccggtgaa gaaaatacgg
      421 gaccattcac cgtgacgagc ggtgataaaa cagctactgt aaatgtgaca aagccagctt
      481 ctggttcttc ttctagcgtt ttttattata aaacaggaga catgctacct gaagatacta
      541 agcacattcg atggtttttg aatattaaca ataacggcac ttatgttgaa caaccagtta
      601 aaatcagtga tgaaatccaa agtggacaaa gattagatcc aagtacgttt gaaattaatc
      661 aaatacactt aggtgaacag aaggtttata gaggagaaga gggaatccaa caatttttac
      721 aagatttccc cagcgctaca tttaattttt cagtaactga taattacatt gaaataacta
      781 taccaaaaaa ctttgtcaat cttaggaaaa taatggtgag ttataagacg atcatagaaa
      841 acccagaaca aataaatttt gagaatcatt cggaagcttg gtttaaagaa tttaataaac
      901 ctgctgttga tggagaaagt ttcaatcata cagttaaaaa tatttcagct tctggtggag
      961 tcaacggtac agttagaggc gaattgaaga ttttcaaata tatcaatgac acagagattg
     1021 gtatcccaaa tgtgactttc gaattaagac gagcagatga acagcctatt caaggacagt
     1081 ctagtatcct gttgacttcg aatgaacagg gagaagctag tatcaaaggc ctgcaagtag
     1141 gagactatgt tgtaaaagaa aaagaagcac ccaattggat tgattttgat ccactaagtt
     1201 caaatgaact caaattctcc ataaacgaaa atgatacaga gggagtcagt ttacctattt
     1261 ataacaagaa aaaggttacg aacatcacag caactaaaat ttggaacggt ggaaccactc
     1321 caagaccgag tatttatttt aaattattca gagcaagtac aaacaattgg gaaccagttc
     1381 ctgatgcaga aacaaaacga ttagacaatg gaataacttc agtgacttgg gaagatatcc
     1441 aacagtatga tgatagtgga aacgaatata cgtttaaagt gcaagaagta gatcaaaacg
     1501 gaaatgatta tgtcccttca ggttatcaaa aaattgaaaa cgggctagtc gttacaaatg
     1561 agaataaaga agtcatttca gtctcaggac aaaaaacatg ggaagacaat gagaaccaag
     1621 acggcaaacg accgacgacg atcacagtaa atctattagc cgacggtcaa ccgatccaac
     1681 acaaagaagt aagtgaaaaa gatgattggc gctatcgttt cacaaatctg ccaaaataca
     1741 gagacgggca agaaatcatc tatactgtaa cggaagacaa cgtgcccgaa tacagtacga
     1801 cgattgacgg gtataacatc aaaaacagtt atacaccgga aaaaacaaat atatctatac
     1861 caggtagcca tataacaccg tggaaaaatt ttccagagaa gacagagaga gagaaaatta
     1921 gaaatctatt ctctcaaaaa ttgcaaatat ttgctcatac aggagaatcc agcgaacaaa
     1981 gaagcaatta tattagtaag agagctgtac ctaaacagat aacaaataaa tcaaaaagca
     2041 ttcttcctaa aacaagtagt aaaaaatcta gttttgctgt aatcattggt tt
//
LOCUS       DAFFSV010000218         2070 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFFSV010000218 DAFFSV010000000
VERSION     DAFFSV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.221"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(366..1658)
                     /locus_tag="LR110_002894"
     CDS             complement(366..1658)
                     /locus_tag="LR110_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL4823804.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          670 a          399 c          331 g          670 t
ORIGIN      
        1 gtatgggcta ctctgtgata cttattagcc attaaactat tttacccaca tagcatatat
       61 tttgaaatcg ttcattccgt aaattcaact aaatatgcct aaaaaaataa aaaatctaag
      121 atagaataag atttattcag tcttagatgg atttatagca actttttcgt aacaacttta
      181 aaaaatacga aaagaaagag ctgttttact ctgactacgt tgctaaattg tcgtttaatc
      241 attcatttaa accaaatctt tttttataga attcgtttta atcctcctga atccaaaaac
      301 tatgaaaacc gtaattaaat agttaagagt ttttaaccac cataattctc tcggctattt
      361 tgaaatcaga taaattttga tcggacacat agttgaataa acctaccgtt gttacggaag
      421 gtaaatcgca tacttttcat tctagaggag aaggtctcgt tgatcattaa ctgattaagc
      481 accatagccc agttttgaat tcgaccacct gaccacttcg cgtgtaattc tttcgtgcgt
      541 aaataaagta gttttaggag tgcattctca ttagagaacg ctcctttttt tgtgactttt
      601 ctgaagctgg agtggacact ttcaaccgca ttggtagtgt acataatttt tcgaatggca
      661 ctaccataat caaatagttg ttcaacatgt gcaaagttcc gtttccagac atctacagca
      721 ccagaataat gagaccaccg attttgaaaa ctgtcaaaag cagcatgtgc agcgtttaga
      781 gaagaagcac cgtagaactt tttcatatct cggcagactt ccttatagtc cttacttgga
      841 atatagcgca atgcatttcg aacaagatga acaatacagc gctgaacaat taccgatgga
      901 aagatcgctt ttgcgccttc ttcgaggcca gaaacaccgt ccatcgaaat gaaaaagaca
      961 tcttcgacac cacgtgcttt cagttcatca aatacttgca tccagcgatt tttagattct
     1021 gtttgattta accataatcc taaaatctcc ttatttcctt tgagatcata gccaagaatg
     1081 gtgtatacag catattcttt ggcttcataa ttttctcgta aagtaacata catacaatca
     1141 acgaatagaa aggcataaca ctttgctagg gggcgggctt gccattcttc caattcagga
     1201 aggacagcgt cagtgatatc tgaaatcatt tcatgggaaa tatcaaagcc ataaatagct
     1261 tcgacggttg cggcaatatc tcgttgactc attcctcgtg catacatgga aagaaccttc
     1321 ccttcgatgt cggagacatc tcgttttctc ttaggaatta actctggttc aaaggaagct
     1381 tcccggtctc taggaacatc aatagctact tcaccaaaac tggttttaag cgttttagtt
     1441 ccatagccat ttcgacggtt atcgtgttcc ttaggctctt tagaatgggc atcataacct
     1501 aaatgattat tcaattctcc ttgaagcatt ttttcaaaaa ggggcccaaa cacatctttc
     1561 aaagcatctt gcatgtcatc gacagattca ggttgatagg cattcagaat ggattcagct
     1621 aacttttcgg catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
     1681 attgtagaaa aagaaaaacc gcgaagcaac cacctgccta ggattaatgg ttacttacac
     1741 ggtttatatt acactctcat cattaaaaaa gtatctttcg atattactaa acaatgaaat
     1801 tatctatttg aaatttaaat ctgataatat attgagtgtt ccaatgaggg ctttgatcct
     1861 attatccata tctttttcag ttttaatatt gatacacatt ataagaaaaa gaaacttttg
     1921 gttaatttat ttcatcccag tgcaaaatat aaaaaagtaa aatagaccat aggcaataga
     1981 ttaacaatta atataataca agcaaatatt ctattcattg tatttctgct ttcaaaaagt
     2041 tctaatattg aaagtataat tccacaaatg
//
LOCUS       DAFFSV010000219         2028 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFFSV010000219 DAFFSV010000000
VERSION     DAFFSV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.222"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            21..980
                     /locus_tag="LR110_002895"
     CDS             21..980
                     /locus_tag="LR110_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL4823805.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            977..1309
                     /locus_tag="LR110_002896"
     CDS             977..1309
                     /locus_tag="LR110_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL4823806.1"
                     /translation="MTIKIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMF
                     SVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDMAKI
                     DQIIQYMF"
BASE COUNT          739 a          328 c          341 g          620 t
ORIGIN      
        1 attataagga gtgagtacaa atgacctata cccatcttac aacggatgaa cttgtaataa
       61 tagagtctta tttcaaaatg aatcaatctg ttgctaaaac tgcccattgc ttgaatcgtt
      121 caagacaaac gatccataaa gtatatctgt tcttcaagca aggaaaatca gccttagaat
      181 attatcaaca gtataagaaa aacaaatcaa actgtggtag acgtccgctt gttttacccg
      241 aggaacaatc agaatatatt caaagaaagg ttgttcaagg atggacaccc gatgtgattg
      301 ttggtcgtgc agcgtttcct attagttgtt ctgctcgtac catttatcgt atgttcaaaa
      361 aggggctatt tgattcttct gacttaccga tgaaaggcaa gcgtaaaccg aatggacatc
      421 aagaaagacg tggaaaacaa actttccgcc gctctattca tgaacgtgaa aaggattata
      481 gccaattctc aaatgagttt ggtcaccttg aaggtgacac tatcgtaggt ctgaaacata
      541 aaagtgctgt aattacctta gttgaacgat tatcaaaagt tatcatcaca ttgaaaccgt
      601 gtggtagaca agcgattgat attgaaaaaa aattaaatca atggtttgaa tctgtaccga
      661 aaaacctatt caaatccatc acttttgatt gtggaaagga attttcaaat tggaaacaga
      721 tcagtaatgt caatgatatt gccatttatt tcgctgatcc aggaacgccg tctcaaagag
      781 gcctaaacga gaattctaac ggattgttac gtagagatgg tttattgaaa tctatggatt
      841 tcaattcagt agatgaattt tttattcaat ctgtcgcatc taaacgaaat aatattccta
      901 gaaaatcact ggattatcga acacctttgg aagtattttt gagttacgta agtattgatg
      961 atctgtctaa cttaatttga caattaagat tcctaaaaaa ggtgatattg tatggattga
     1021 ttttgatccc tccgtaggaa aagaaattca aaaaagacga ccaggactag tcgtctctcg
     1081 ttatgaattt aaccgtaaaa caatgttttc ggtcatatgt ccaattactt ccactataaa
     1141 gaatttaccg acacgttact cattacctga agagctagat ataaaaggac aagtaattat
     1201 tacacaatta aaatctcttg attttaagga acgaaaactc aaaaaaatag aaaacttacc
     1261 attgcaagac atggctaaaa ttgatcagat tatacagtat atgttttaag cattgtaagt
     1321 aaactctata atatttttat atgctaaaca taacgaaatt aaaaaaaggc caagtaaatg
     1381 tcgttcattc tactagtgtg gtcgatggca ttaatttccg tatcttaaac taaagaatat
     1441 atagcatttt aaacgaatga ttgaggtgat ttcctcaagt cattcgtttt tttgtgtcac
     1501 aaaacttgtt ttttcacgac atatcttaac gtatacgaaa agtcgcgaca atactttaac
     1561 agaaagaaga aaaagaacaa ctgacagttt tagtcctaaa aaatctaaac tctttagata
     1621 tgagtaatgt cattttaaac tatatcgatg ctcaaaaacg taaaatttgg gatcaagatc
     1681 tgtctaggaa agaatttcta tagtttaact gaataacaca aaaaggcata tcatgcgaca
     1741 agaaaaacaa tgaaaagaga aatttgccat cactggaaca tccatacaga aattagcata
     1801 gattctgaaa aatctaccgc tagtttttat ttagttgttc taacgacacc aatacttgta
     1861 atgatcgaaa atgtgcaatt gggtcagata gtgaaccaaa gtagaatctc aaagcgacaa
     1921 ttatgaatgt aaaagtacct tttatgatta gtttattctc taattgatta catcccgtat
     1981 aattaataaa attactttac cgattacacg atcttatgtc agtagagc
//
LOCUS       DAFFSV010000220         1959 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFFSV010000220 DAFFSV010000000
VERSION     DAFFSV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.223"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..425)
                     /locus_tag="LR110_002897"
     CDS             complement(<1..425)
                     /locus_tag="LR110_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL4823807.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="LR110_002898"
     CDS             complement(534..1565)
                     /locus_tag="LR110_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL4823808.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          603 a          324 c          339 g          693 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatt
//
LOCUS       DAFFSV010000221         1929 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFFSV010000221 DAFFSV010000000
VERSION     DAFFSV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.224"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            100..336
                     /locus_tag="LR110_002899"
     CDS             100..336
                     /locus_tag="LR110_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBL4823809.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            355..570
                     /locus_tag="LR110_002900"
     CDS             355..570
                     /locus_tag="LR110_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HBL4823810.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1273..>1929)
                     /locus_tag="LR110_002901"
     CDS             complement(1273..>1929)
                     /locus_tag="LR110_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL4823811.1"
                     /translation="FKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAE
                     VIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQER
                     IDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISEL
                     EKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV
                     "
BASE COUNT          628 a          299 c          345 g          657 t
ORIGIN      
        1 actattcaag aataaacttg ataagtttca ggaagatttt tactgttaat caaatacgta
       61 tgttacagta gcaccgtaaa aaatattatt taaagaagcg tgtttaaaca tgcaaaatgt
      121 aaaggaacta agtattgtgg aaatgcacaa actgttggtg gaggcaataa tgcttgggga
      181 aaattggggc aagttgtagg tggacttacc acaggggcag taggtggtgc aggtttagga
      241 acagcaattt gcggaccagc ttgcggtgtt gttggagggt tatatggagc tgtcgctgga
      301 ggtgctgcag ctggctggga tgctcgaaaa aaataaaaaa ggagaataag tagtatgcat
      361 attaaaaaca caaaaacaac atttatatta tcttcagaag agttgaaaaa tattcaaggt
      421 gggagtgccg tgggagtttt aggcactact ttttctgggg caacagcagg agtaaaactt
      481 tgttcagccg gaggacctta tgcaatagct gcttgtggtg taggtggagc tttattaggt
      541 gctggattca gcatgtggac cggtgcgtaa acaattaaaa aatatcaaac ctcaagattg
      601 gtttgtgtcg attacgaaca taggtatgtt ctttatcttt tcaaaagttt ggaatggaca
      661 gaacttttta ctaaagcttc ttgtgctagt ttttataatt atattaaatt tttatctttt
      721 attaaagtca gataaaaatt ctttggaaat gtaatggtag agatattttt acaaaaggac
      781 ttgcagcgct gtagttggtg ctgcaggagg acctatttcg gccgttacag gaggtattgg
      841 taatgtactt gtcggatgtg cattaaagcc tgttcctgca agttaaattt ataaaaacag
      901 gaaagtaatt atctcaaatc ctatcaagta caatcatagg attgcacttg acaggacaaa
      961 ttttttaact aaaaaatgaa aaacactttc taaaaattag tagaatttgc caaatctttc
     1021 gcatcttcca ccatacattt aattttaatt cccctttatg gtacttaata aaaacaagaa
     1081 aagaactatt catttggccc ttttttggtt ccagaccata gaaatattcc tccagtaact
     1141 aatattgctg ttatgcacgt aactaataac agctataaaa attggtacaa ctttaatagg
     1201 attcagaaaa atttgcacta gtgaaggaat ctattttatt agaaaaatgc aatctcccta
     1261 aagaaaaaac cactaaacag tgctttcttt caacttctcc ttaatttttt gtgctttccc
     1321 taaagtaaag tttctggcca actctgaatc aaaatatagt gaacgttttt taaaatctat
     1381 ttctttaacg ttaaagatat ttactaaaca cgctcgacta attcttgtta acataggata
     1441 tttcttttca agctcactaa ttcttccata aaattcgtac tgcccatctt tagtacacaa
     1501 tgataatcta tgtggtaatt ttgaagcttc tacaaaataa atgtcattta tatcaaaagt
     1561 aaatgtttga gaacctatgg aaaaaacaaa tgcttgattc gtaacctgtt ttgttgcatc
     1621 tatcctttct tgcgctaata acaataactc aacaatctcg tttcgatact catctagtgt
     1681 ttgatctttt gttacaaatc ctaacgtttc aactcttctt tttattgtta cgggtaacat
     1741 ctcatcatga gtagttgtaa aaatgatttt tgcttgaaca tcatattttc tgattacttc
     1801 tgctaattct ataccgttaa cttcatgatt taaatcaata tctaagaaat atattccttg
     1861 ctttggtcgg aattgtttga ggtatttttt aacttctaac ggactttgtg ttttcaatac
     1921 tatcttaaa
//
LOCUS       DAFFSV010000222         1929 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFFSV010000222 DAFFSV010000000
VERSION     DAFFSV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.225"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1023
                     /locus_tag="LR110_002902"
     CDS             <1..1023
                     /locus_tag="LR110_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL4823812.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1034..>1929
                     /locus_tag="LR110_002903"
     CDS             1034..>1929
                     /locus_tag="LR110_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL4823813.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMN"
BASE COUNT          696 a          341 c          414 g          478 t
ORIGIN      
        1 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
       61 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
      121 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
      181 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
      241 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
      301 gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta cgttgaagag
      361 tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat atgtgtggac
      421 aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat cgttgatcct
      481 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
      541 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
      601 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
      661 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
      721 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
      781 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
      841 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
      901 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat ccagcaacaa
      961 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     1021 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     1081 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     1141 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
     1201 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     1261 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     1321 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     1381 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     1441 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     1501 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     1561 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     1621 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc aggaaaagat
     1681 tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg
     1741 cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc
     1801 ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga
     1861 aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa
     1921 aatgaatca
//
LOCUS       DAFFSV010000223         1922 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFFSV010000223 DAFFSV010000000
VERSION     DAFFSV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.226"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            39..1151
                     /locus_tag="LR110_002904"
     CDS             39..1151
                     /locus_tag="LR110_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL4823814.1"
                     /translation="MLFSLSAFSTGTILGWQFYNNEHSQIVAHAQSAQKKLILTTNEQ
                     QLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQIGSVQKA
                     LTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTIPMTD
                     EEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLGLQHT
                     KFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTLFTQV
                     LSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDSQDAI
                     IMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1418..>1922)
                     /locus_tag="LR110_002905"
     CDS             complement(1418..>1922)
                     /locus_tag="LR110_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823815.1"
                     /translation="ILAISILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVP
                     IGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTLKSSKGKTIVLPVFRYKGLTVT
                     VNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKL
                     KIRTSHE"
BASE COUNT          671 a          330 c          293 g          628 t
ORIGIN      
        1 ctaaaaaaag aaaatattca tttttaatga tcgttgtcgt gttgtttagc cttagtgcgt
       61 tctctacagg aactattctt ggatggcaat tttacaataa tgaacactct cagatagttg
      121 cccatgctca atcagctcaa aaaaagttga tactgactac caatgaacaa cagctcgttg
      181 aaaaaatcga tcaactatta agaaaaaatg attatattgg ctcaatttac gtaaagaaaa
      241 ataatcgcat catcatggaa aaaggctatg gctatgcgaa taaactcacg caacaaccaa
      301 atgatccttc tctttattat cagattggtt cggttcaaaa agcattgact actcttttga
      361 ttttaaaaca agtcgagcgt gggcaccttt ctttagatac taagctggct caattctatc
      421 cacaaattgc tggcagccag caaattacta tccgtgactt gctgtatatg cgctcaggct
      481 tgcaccgaac tgcttctcca accataccga tgactgatga agaagtcgtt caatttgcga
      541 ttcagcactt aaaatttaca gattatcaga cctctcatta tgattcattg aactttacca
      601 tattgactgg aattttgatc aaattaacaa atcaaccgta tgaaaaatta ttgaccgatg
      661 atatcatcga acctttagga ttacagcata caaaatttta tgaccaagtc aaaaattcat
      721 ccaatcatgc actatcctat caaatgtcta aagaggatga ttactttaaa ccactgacag
      781 aatctgaaac agagatccat aacgaattag ggactggtaa tatcagtatg tccgtctatg
      841 atttaaatac gctgtttaca caagttttat ctggaaaact agtttccaaa gacttactct
      901 tctcattatg gcgtgaaaat gcgaatggac atccatatag tggtggggta tatagcggag
      961 ataattatat cctgtcgcaa ggaaatatcg actcttttga tacatctgct atatttagca
     1021 aagattcaca ggatgctatc atcatggaaa gcaatataaa agctgacaaa actatcaaac
     1081 tgcctactgc tgatctaaga aaccaaatct atgacctgat ggaaggaact actaagttgg
     1141 caaatagctg atatgatgaa aagatagcaa atactacttc tttgaatagc atttgcttaa
     1201 aaaaagtact cgactaaacg taggaaagag tgtgggacaa atctaaaaat caatttttgc
     1261 tacagacttt aattccaaat aaacagaaca gaaagaggtg ttcttctttc tgttctgtcc
     1321 aaattattct aagatttctc tttaccaaca ttatttgaca aagggcctat taaatctaaa
     1381 atagtatctt tagattgcta aaatatgtgc tagcaaatca ttcatggctt gttcttattt
     1441 ttaattttat gcaataaaaa agaagaacaa ttagcgtgat tatagaaata aataatgaga
     1501 ttttagaaaa aaatgtgtat gaatacctga ttgttacttt atttattcca ctttcacttt
     1561 ttactgtaac aaaaggacct ccttgtttta gtactgtagc attttttgaa ttaacagtaa
     1621 ccgttaatcc tttataccgg aatacaggta aaactattgt tttgcctttt gaagatttaa
     1681 gcgtaaatgt aatttcatct ttatttggag aaaagctact ataaataact tgaccatctt
     1741 ctgtaacttg atggtttttc aaaagcaatt gatctttttt atagcctata ggtacataat
     1801 caaaattctc aaattcttca agtttatttt ccatttgttc attagaataa gttccttgaa
     1861 aattcatttc tttcaccatg ttaactactg atgaataatt taaaattgaa atagctaata
     1921 tt
//
LOCUS       DAFFSV010000224         1920 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFFSV010000224 DAFFSV010000000
VERSION     DAFFSV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.227"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1014
                     /locus_tag="LR110_002906"
     CDS             <1..1014
                     /locus_tag="LR110_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL4823816.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERSNGTYDFDRFYQGKGDINA
                     QILFSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQ
                     ERE"
     gene            1025..>1920
                     /locus_tag="LR110_002907"
     CDS             1025..>1920
                     /locus_tag="LR110_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL4823817.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSLLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMN"
BASE COUNT          682 a          344 c          409 g          485 t
ORIGIN      
        1 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
       61 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
      121 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
      181 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
      241 tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca gttaaacgat
      301 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
      361 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
      421 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
      481 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
      541 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
      601 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
      661 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
      721 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg ctcgaatggt
      781 acgtacgatt ttgatcgttt ttatcaagga aaaggggata ttaatgctca gattcttttc
      841 tcttataaag cagaacaata ctacaaaggc tataaaaagc atgaacatga atttgtacct
      901 gaagtgaaaa aggattggaa cgatcaattg aagcatgaga tccagcaaca agaaattaga
      961 aagcaaaaac gtgcgatgtt gtttcaacaa ggcagacaac aagaaaggga gtgagatcgt
     1021 cacgatgaaa actgtcattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     1081 cttttctaaa gccacccgaa aagacgggta ttttgagatc caagatcgat tgtttcctgg
     1141 tgaaactgtg attacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     1201 cgatgaaaat tggaaacaat ggtcattaga acatttacct attttcccta cacattatca
     1261 ctatcatgtt cctaaagata agaaaaagca attcaacgtg gtgaaacagc aacttcaatc
     1321 ggccgacacc attattattg cgacagatag cgatcgcgaa ggggaactga tcgcttggac
     1381 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     1441 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     1501 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     1561 tagcctactg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     1621 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     1681 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     1741 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     1801 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     1861 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
//
LOCUS       DAFFSV010000225         1852 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFFSV010000225 DAFFSV010000000
VERSION     DAFFSV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.228"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1087
                     /locus_tag="LR110_002908"
     CDS             <1..1087
                     /locus_tag="LR110_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL4823818.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..>1852)
                     /locus_tag="LR110_002909"
     CDS             complement(1305..>1852)
                     /locus_tag="LR110_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL4823819.1"
                     /translation="FYEEFYKRLMRFIFVTGVRSEELLALQWKDFDLINGRCSVNKSI
                     YIRTRQDYEFAETKNTSSIRIISLDRQTVEDLKTWKKTQQSIGKNIELVFSFDGLPPS
                     PRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFLIEHNKNIYAISKRLGHASIKTTLDK
                     YGHLYPETNQALANEITRFDI"
BASE COUNT          637 a          328 c          328 g          559 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa at
//
LOCUS       DAFFSV010000226         1841 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.229, whole genome shotgun sequence.
ACCESSION   DAFFSV010000226 DAFFSV010000000
VERSION     DAFFSV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.229"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..135
                     /locus_tag="LR110_002910"
     CDS             <1..135
                     /locus_tag="LR110_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBL4823820.1"
                     /translation="FIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY
                     "
     gene            108..785
                     /locus_tag="LR110_002911"
     CDS             108..785
                     /locus_tag="LR110_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL4823821.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            736..>1841
                     /locus_tag="LR110_002912"
     CDS             736..>1841
                     /locus_tag="LR110_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBL4823822.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGL"
BASE COUNT          666 a          247 c          380 g          548 t
ORIGIN      
        1 tttataaaaa gcttgaaaaa tgttaagaac atctacggtg acggcaacag ttctagaaga
       61 ataacaaaaa cattgaatca aatattgaat gatggggaag atattaagtg gattcaaaaa
      121 caaatagctt attaaaatat actagtgatc catacattat tattggggct ggcggacacg
      181 caaaagtcat tattgaaacc ttaagattag aaaataagtt aatatatgga ttttttgatg
      241 ataagcaaaa taaaatgcaa gatctgaaat atcttgggaa aataaacgaa ttgaatagct
      301 gggcggatag caaaaaaaaa atctataaat ttattgtagc tattggtgac tgtgtagtaa
      361 gaaaaaagat tataaaaaaa ttgaatatgc caagtgagca atatggaata gctgttcatc
      421 caagtgctat agtggcaaaa gaaatttcaa ttggttatgg aactgttatt tttgcgaatg
      481 ctgtaattaa cccagatgct gtaattggag agcatgctat aataaacaca ggtagcattg
      541 ttgagcatga ttgtagaatt aataactatg ttcatttatc tccaggtgta tgcttagcgg
      601 gtggagtaca tgtaggtgta ggaactcaaa taggaattgg ttctcaatgt atccagatga
      661 agaaaattgg atcttggtgt atgataggcg cagggagcac tattgttaag gatattccct
      721 cacattcctt agcttatggc aatccagcta aaattaagaa agaaggaatt aattttgaat
      781 tctagaattt tattatcaag tcctcatatg ggaggaacag aagaaaaata tgttgctaag
      841 gcttttaaag ataattggat agcgccatta ggtgaaaatg tggataaatt tgaagaaagt
      901 attacaaaat atactggttc taagggcgga gcttctgcgt taagttcagg tacagctgcc
      961 atacatcttg ccttaagact gcttaatgtt ggaaaaaacg atattgtctt tgctccttca
     1021 tttactttta tagctacagt aaatcctatt ttatatcaag gggctgtacc agtgttcatt
     1081 gactcagaac ctaatacatg gaatatgagc cctgttgcac tagagagcgc attagaagaa
     1141 gctgaaagaa acaaccaatt acctaaggca atcatcgtag tgaatttata tggtcaaatg
     1201 gctgattatg aacgaatttt agctattgca aaccaattta atgttccagt gatagaagat
     1261 gcagcggaat cgttaggttc tacttataag ggaatttcgt caggcttatt tgggacaatt
     1321 gggatctatt catttaacgg taataagatc atcacaacct caggtggagg agctctagtg
     1381 tctagtgata aaaaatatgt tgagcattca agatttttag caacacaagc aagagatcaa
     1441 gcaccgtatt atcaacatag cgaaataggt tataattacc gtctaagcaa tatttctgcc
     1501 ggtatcggac gtggacaaat ggaagtattg aatgatagag tagctaaacg aagagaaatt
     1561 ttcaatcgat ataaggaagc tttttctgga attgatagaa ttgaaatgat gccagaagtg
     1621 gatggcatgg tgccaaatcg ttggttgtct actttaacga tcaatgatta taattctatt
     1681 gatcaagtag ttaacgtaat aaataaacta aatgaaaaaa acattgaaac aagagctttg
     1741 tggaaaccac tacatttgca accagtattt caaggggtaa aaatgtatta ccatgataat
     1801 aatcttagtg tgtcagaaaa gttatttaat agcggcttat g
//
LOCUS       DAFFSV010000227         1826 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFFSV010000227 DAFFSV010000000
VERSION     DAFFSV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.230"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(376..873)
                     /locus_tag="LR110_002913"
     CDS             complement(376..873)
                     /locus_tag="LR110_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL4823823.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(913..1722)
                     /locus_tag="LR110_002914"
     CDS             complement(913..1722)
                     /locus_tag="LR110_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL4823824.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          523 a          341 c          316 g          646 t
ORIGIN      
        1 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt
       61 ttttacttgg ctgttcttat tactgcagat gcaagctttt gaataccgtt taaggaagag
      121 gttgtgaaat cagcgttttg acctgagtat tagcttaaca aacggaaaaa tagttcctca
      181 tattttgccg tttgttaagc taataccgag ggtcagctga ttgaacacca tttaagaaag
      241 aggtcgtgag cctgccgttt cactctgtgt cataaaaaag ccaatctact ttgatagaaa
      301 aagcacgtag gactaaaaca gttctgcgtg ctttttttat tgtgtgttaa atatcgtaaa
      361 tgaagataga tatttttatg attcgacgat agagacgttg aaatctttca acagctctaa
      421 agcggattct ccgtgttttc tactggagac taacattgga tcaacaaaaa agtctgcttc
      481 aggaaaagca ctctgcaggc ctatcagatt agagagtaca cacaagtttg tttcaatccc
      541 gactacttcg atctcttttt gttcttcttt gctttcaaat aaagtttcct ggatttcagc
      601 taaagtttca ggtggaatcg taaagtaata ctttttaatc actcgttcat tttcttgtgg
      661 agaaagaaga gggatcaatt gcaaatcttc tcgttcttct tctttgtcct tcagttcaac
      721 tggtatatcc cgtgtgaaga gaatatattc attattttgg cgtgctttag ctaatctttg
      781 agcaatacgt tctgccaatt gatctgactc tggaatataa aattcgctgt ttggatcaag
      841 aatatgattt tgcatatcaa taataactaa cattttcttt cctcctacat tcatatcgtc
      901 attttattct ttttaataga agttttttaa aatataagca acaccagctt cttcattcgt
      961 cagtgttact tggtttgcaa tttcttttac ttgtcgctgt gcattcccca tcgcaatact
     1021 caacccgctg taattcaaca tatcaatgtc gttttcttga tcgccaaaag ccatgatgtc
     1081 actcgattcg attccaaggt aatccgccag aaaaacaagt gaatgagctt tagtgatacc
     1141 tgtaggcgtg atttctaaga aatgcggatc agagaatact gattggatac catctgcttg
     1201 cagatctctt tgtagcttct gtagtttttc ttcactctta tctgttaatg ctaatttaag
     1261 atagctaaga gaaggatctt ccaacatatt taaaatttga tcaaaagaat ggttctgtaa
     1321 attgaaatgt tcataataag ggtcatcttt gactagattt gattcatcga atgtcagctg
     1381 atcatcttgg gtttctcggc tagcaatgat ggatattttc gcttcttgag catagaagaa
     1441 ggcttttctc agtgtatcgc tatctatttt tgaacgtctg agaactttcg ccatttttcc
     1501 atccttcatt tcagcaatca gtgaaccatt ggaagaaatc aaatagccac cgattcctaa
     1561 ttcggaaaaa atatctttag cagaaatgct gttccttccg gtagcgacgg ttacgattcc
     1621 gccattttca tcaaatttcc gaagtgcttg gatattttct tctggtatct ttccatttcg
     1681 atcaagcaag gtaccgtcta aatcaatcgc tgctagtttc attttatctt cctactttct
     1741 ttttacatat ctattttcct gttaccttaa gattaaatga gaatttaaaa aaatgaaact
     1801 aacttgcttt gtcattatga attttt
//
LOCUS       DAFFSV010000228         1740 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.231, whole genome shotgun sequence.
ACCESSION   DAFFSV010000228 DAFFSV010000000
VERSION     DAFFSV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.231"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(79..531)
                     /locus_tag="LR110_002915"
     CDS             complement(79..531)
                     /locus_tag="LR110_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823825.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSVEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(538..1086)
                     /locus_tag="LR110_002916"
     CDS             complement(538..1086)
                     /locus_tag="LR110_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL4823826.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(1131..1463)
                     /locus_tag="LR110_002917"
     CDS             complement(1131..1463)
                     /locus_tag="LR110_002917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823827.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1477..1734)
                     /locus_tag="LR110_002918"
     CDS             complement(1477..1734)
                     /locus_tag="LR110_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823828.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
BASE COUNT          585 a          368 c          183 g          604 t
ORIGIN      
        1 aatctcccca aaaagacaca gcattaagag atataccaac acaacagtgc aaataactct
       61 acatcttcct tcgcaacctt atttcaataa accataaaaa ttatttttta atagaaactg
      121 aaaattaaaa ctagaaaaag tagttttacc gaatgtaaga gcatcatcac catcaatata
      181 gaaatacatt cttttccagt tacatttaat ttttaaacaa aattctcccc aaaaaggaac
      241 tttttctact gaaaacttgg taatctcttt gtaattaatt cttgttaaag aataattaaa
      301 cacttcattt ttacttgtta ttttttcatt gactataact agttcatctg gtaaaaaaat
      361 taaccaacaa acatcgacaa acagtaaatg atataatcca tttccacctc caagcatatt
      421 caatactggt gaacatgcct tagaaacacg aatatagtta tggtttaaat agatattttc
      481 tttaaacatt gcttgtttaa aagccatatg ctcttgttct aaacttttca taaactttta
      541 ctctaatgca cagctagcag cagctgctgc aattcctgct acaatattta ccttagctaa
      601 ctctttagct gcagcttttc ctaattgttt ttctgcaatt tttaataata aatccgcagc
      661 tttttcaaaa ttaaaagctt ttaatgcttc aataaaatct ccctgcgtac ctatagccca
      721 agccactcct cctccaggaa gagaattaat ggctacacat ccagcatatc gtttccaatc
      781 aactttctcc attctaggaa caataatatg ttcataaaag actcttgctg ctcttccttg
      841 ttcgttatct tgactgattc gttcacgaat agcaccaaaa tcctttatat ggtatacacc
      901 atgttcgtct aaaaaaccaa ctgtatcaaa ataaaaagtt aattcatcta ctaatttatt
      961 ctttatttct aaatcaactg tattcacttt ttctgtggca tacacacttg ttccagtact
     1021 aacaaaagta cttaaaccta ataagaaaac acctaaaaca ccaaatattt taattattct
     1081 tttcataatt tctcctccat aaaaaataag ttacttttta aatatcttgt ttatgaattc
     1141 attccccaaa ggccttttaa gattgataca gcagtccgtt cacttctttt agacttgtca
     1201 tacaagtctt ttaaatgatc ccatatcaga ctagtttgtc tatctagctt caatccatta
     1261 aacgctaatg aacggttcat tttttctatt actttagata aagctacaga aatttttata
     1321 ccttcacaac gcgcatcttg gatttcaata atagtatcat caattagttt ttgaatttct
     1381 tgcctatcaa catattcaat cttagaaaga gaatctttta aagctactaa tgattcaacg
     1441 atttcttttt tctcttcaga cattttcttc ctccttctag tgacccgtaa agccatgtag
     1501 agcagagtcc attgaaccag taattacact atctacaacg ccattccaat ttactctaca
     1561 ttttccattt ttcagatttg tcggtccata cggatagcct ttttgacatt caatgttcgg
     1621 attataccag ataggattat accattcagc cgcagaagca gattcagtcg atacaaacaa
     1681 tcccacacca acaaatccaa tacacatcac actatagcca attagttttt tcatctcgta
//
LOCUS       DAFFSV010000229         1722 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFFSV010000229 DAFFSV010000000
VERSION     DAFFSV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.232"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            466..1065
                     /locus_tag="LR110_002919"
     CDS             466..1065
                     /locus_tag="LR110_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL4823829.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1129..>1722
                     /locus_tag="LR110_002920"
     CDS             1129..>1722
                     /locus_tag="LR110_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HBL4823830.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGDPVLLQSF"
BASE COUNT          615 a          261 c          326 g          520 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat aatgatgaaa tcatttagtt
       61 cttataaatt agaaaagaga aaataaatat tccatatcta ctttattgga tccgtaacat
      121 gactaaattc tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt
      181 atatagactg aaaattttga attttcagaa tagaataact taaaagtact gacaaaattt
      241 tttaaagggg caatattaat gttctgagat aatcaaatcc taccgaaaat atcaaaaatc
      301 caatgtgaca caatcagatt gtgtctcatt cgagggaatc acacaatccc acgtggctct
      361 aggcagaatt gaatttttgc ttggggcttt ttccttatat tgattttgaa tgagacttat
      421 tatatgttat aatgcaatta agtatcattg gagagggcga agaaaatgaa gacggtagaa
      481 cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa tcttggcttc gggaaaatat
      541 ggccaacgaa atttggttct tttttcgatt gggatcaaca ccgcctatcg catttctgat
      601 ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac gcggtcgcgt ggtggtcaaa
      661 gatcgattag cgatgaagga acaaaaaaca gctaaacata attcggtcta tatttccaac
      721 aaattgcgaa aaacgatttt ggagtacgtt caacaggaat ttcctgatca actccttcag
      781 caagattttg acggttattt gttccctagt cgaaaaggga cgaatgaacc gctcaaccgc
      841 caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa tcggattgaa agacattggc
      901 ccacattcca tgcgaaaaac gttcggttac tttctctata aacaggggac caaaacagaa
      961 atcattcagt ccttgttgaa ccattcttct cagcgcgaaa cattgcggta tattggtatt
     1021 acacaagaag acaaagatac agctgtaaaa agcttagatt tataatataa aagttaagtt
     1081 taaacatgga tagttttaaa ttagtagatt gagtgttggg aggaaagaat gagtacatta
     1141 tatattatag gaaacggttt tgatttaagt catggtttaa aaactagata cactgatttt
     1201 tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata aaatagacac aatgataaat
     1261 gaaaataatg aggtgagaag tacagctgat gataagatag gttggtttaa ttttgaaagt
     1321 agtttgcatg agttaattca aagtccagtc tttgaagatt atgtagaaga gtctaaggat
     1381 tatctcatag gttatggaca tgaagattgg tcagattctg cccatcatga ttttcaaaat
     1441 atggtttatg attatgttgt ttctctagaa gaattacaac aacttttcaa ggaatggata
     1501 cgcaaagaaa tacagccgac aatagaacat aaaattccaa aataccaatt ttcgtccgat
     1561 gtactattct ttacattcaa ttatacagat gttttggaaa ttctatacaa gcaaccgaaa
     1621 gaaaacatat atcatattca tggcagttat gacgatataa taatcggcca ctccgagaat
     1681 tttgacaact ttataggaga tccggttctg ttgcaaagtt tt
//
LOCUS       DAFFSV010000230         1713 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFFSV010000230 DAFFSV010000000
VERSION     DAFFSV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.233"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            154..750
                     /locus_tag="LR110_002921"
     CDS             154..750
                     /locus_tag="LR110_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL4823831.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(808..1290)
                     /locus_tag="LR110_002922"
     CDS             complement(808..1290)
                     /locus_tag="LR110_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823832.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1396..1593)
                     /locus_tag="LR110_002923"
     CDS             complement(1396..1593)
                     /locus_tag="LR110_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823833.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          568 a          350 c          264 g          531 t
ORIGIN      
        1 ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat tttttgtgag
       61 catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc aattaaaaaa
      121 atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga cactttgaaa
      181 aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga agaattaaca
      241 actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca atcaacacgc
      301 gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga agaagcactt
      361 cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc tggtttcaaa
      421 aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg tctacaagaa
      481 caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa cggaatcgca
      541 acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt gcaacattac
      601 aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa ctggaaacta
      661 cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga atacatgaac
      721 gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac agaggttgtg
      781 acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat caatctctgc
      841 ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg cagaaaattt
      901 cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt tagaaccttt
      961 tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt tgtcaaagct
     1021 attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac taataaaaat
     1081 cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga tgagtgtata
     1141 ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat ttcgcacagt
     1201 ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa caacgaataa
     1261 aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt cttctccaga
     1321 aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag cacgccgcac
     1381 gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc ttgctttttc
     1441 atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg cgcagtttgt
     1501 catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt tgcatgcata
     1561 ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact gctggaactt
     1621 ttctccacat taccactgct atttctttgg agatttaact cattgatttt tatttacagt
     1681 taaaaatcaa ccttacaggc attatagcgt act
//
LOCUS       DAFFSV010000231         1698 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFFSV010000231 DAFFSV010000000
VERSION     DAFFSV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.234"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..903
                     /locus_tag="LR110_002924"
     CDS             <1..903
                     /locus_tag="LR110_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBL4823834.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            916..>1698
                     /locus_tag="LR110_002925"
     CDS             916..>1698
                     /locus_tag="LR110_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL4823835.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFS"
BASE COUNT          564 a          248 c          389 g          497 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagacgga acttatggac atactggcgt tatccgaggc
      781 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
      841 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
      901 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
      961 tacgtgggga tgattcaagt tcgacaaggc aataaaaaca ctgaagttat ggaagctaca
     1021 attgtcgaaa atggtcttcc atatgattta tcagaatgta aggtatattt tgaatcagtt
     1081 gttggcggaa aatatccagt ccaattagaa acaagaattg tggatgctaa aaaagggaaa
     1141 atcaaatata tttttgataa atattccatg cagtgtttgc atcgacaaac agccaatttc
     1201 attatattta aaggagaaga cttgattgga acaactcaag acttctccta ttttgtcatc
     1261 aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat tgaagattta
     1321 attgctgata tgacagattt tattaatgaa aataaaggcg attttacgga ctggatgaat
     1381 gaaaggaaag aagaatttga tcgctggaga gaagaacagg agaatagttt ccaagattgg
     1441 agagaaggac aagaatcaga ttatttgtca tggtttgaat caatcaagga tattttgaag
     1501 tctatcgatc caggtggagt aatgttagcc gaattaatgg atgcacgtgt agatcttcaa
     1561 ggtgtacgcc atgaatcgat ttctgaacgt ttcctatccg atctaaatta tttgtatcaa
     1621 aaaatgaaag cagcactttt cacgattgaa tacggtgaaa ttgaagtgac tgatattctt
     1681 caagacgatc tcttttca
//
LOCUS       DAFFSV010000232         1669 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.235, whole genome shotgun sequence.
ACCESSION   DAFFSV010000232 DAFFSV010000000
VERSION     DAFFSV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.235"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(109..486)
                     /locus_tag="LR110_002926"
     CDS             complement(109..486)
                     /locus_tag="LR110_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823836.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(786..1667)
                     /gene="galU"
                     /locus_tag="LR110_002927"
     CDS             complement(786..1667)
                     /gene="galU"
                     /locus_tag="LR110_002927"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL4823837.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          464 a          370 c          245 g          590 t
ORIGIN      
        1 agattctttt atataagtga aaaaagcttt tttccaccaa ttaaataaat ctctcatctc
       61 aatagtttat ccattatttt atcttttttt gcgtatacct actaaatatt accgtcttct
      121 tctcgttctt ctactttgtt cgagagaatc tgtactagaa aaagtagttg tatgtctacc
      181 ttttctgcct atccgagact ttcttttcat atatgggata taaatgatta gatacagtac
      241 agcaaaaaat aaagcaaata cgcaagcttc ccaactacct aacgccaatt caaccatcaa
      301 ggtagcaacc ccaacggatg ctgcatacca gataaaaatc agaaacgttt ctttttttct
      361 gaccatgcga aacatgataa atagactaat aagcaatagt ggcaacacaa atatccaatt
      421 gcttccttcc atcagccact tgagtatatc aagcagcgac tgtgcaacag cgactaattt
      481 tcccatagtg aacacacacc tttttttata attaaaaaat tcgccttttt attctactat
      541 gaatcttctg gcttggcttt catttctctt atgtaatcat tttcttttat acaacttaaa
      601 aaaaaattaa accattagca taatattttc tgaatctatt cacgaaagtc tttttctatc
      661 tttcgttttc cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa
      721 tgacgcacaa gctgccgctt taataacaaa tagcctttca ttctcagcag gccacttatt
      781 tccagttaac tttctgttct tagcttttta ctcaactgga taatatatgc acttaatgaa
      841 gtcccaattt ccgaatgttg aagtccatat tcaatatttg tcttcatata tcctaactta
      901 tcgcctacgt cgtatctttt tcctttaaac tctctcgcat agaccgcctc tgtctggtta
      961 agagtctgaa gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc ttgagtttct
     1021 aatatctcaa atattttagg agtcagtaga taacgtccga taatggctaa attacttggt
     1081 gcttttccca tttcaggttt ttctacaaac ccttttactc tgcaacgacc cttttcgcta
     1141 tctgttttag gatcaataat tccgtattta gatgtttcat tttctggtac acgcatgacg
     1201 gcaacagcgg aagcttgtgt ttcttcataa tccatcatca attgttgagt caatgggata
     1261 tcatctttca tcaaatcatc ccccagcatg acgacaaacg gttcatttcc tataaatgct
     1321 tttgcttgaa gcacggcatc ccccagtcct cttggatgag attgacggat gaagtgaaga
     1381 ttgacttgag tcgtttcttc aactaaagcg agtaattctg ttttttcctt ttcccgcaaa
     1441 ttgttttcca attcgatatt tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc
     1501 gttacgatca agatgtcttc gattcccgaa tccaatgctt cttccacaat aaactggatc
     1561 gttggtttat ccacgattgg aagcatttct tttgccatgg cttttgttgc cggcaaaaaa
     1621 cgtgttccta gcccagcggc aggaatgact gcttttttga ccttcactt
//
LOCUS       DAFFSV010000233         1665 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFFSV010000233 DAFFSV010000000
VERSION     DAFFSV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.236"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..964
                     /locus_tag="LR110_002928"
     CDS             <1..964
                     /locus_tag="LR110_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HBL4823838.1"
                     /translation="EKSNNVIIGFLFTKQSGSIKDYKGTPRTVLVQRHDELYLVNRTS
                     GENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAENLYRNSLGADSLK
                     NARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFFKEYDYVFVCIGDT
                     MASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFNCLYTDSNSKNRCS
                     NRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKVNQNNMTNSTLFSW
                     IGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            957..1418
                     /locus_tag="LR110_002929"
     CDS             957..1418
                     /locus_tag="LR110_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HBL4823839.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            1431..>1665
                     /locus_tag="LR110_002930"
     CDS             1431..>1665
                     /locus_tag="LR110_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823840.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVIS"
BASE COUNT          575 a          220 c          330 g          540 t
ORIGIN      
        1 agaaaagtca aataatgtca taattggatt tctttttaca aaacaatctg gatctataaa
       61 agattataag ggtacgccta ggactgtatt agttcagcgt catgatgaat tatatttggt
      121 gaatagaacg agcggagaaa actatggatt gggtgctaat atagctattg ttggggtagg
      181 gtcagttggc agtaaaattg ctgaaaatct tggtccatta ggtgtgagaa agatgacact
      241 catagacaat gattgttttc aagctgaaaa tttgtatcga aattcacttg gtgcagatag
      301 tttaaaaaat gcccgatctc agaataattt ttttaaagtt gatttaatgc aggaggagct
      361 cgaaaaaaaa ttcccatacc tttgtgttga agtggagtca atgaacgttt tagacttaat
      421 tagtatgcaa cctggttttt tcaaggaata tgactatgtt tttgtttgca taggagatac
      481 aatggcaagc ctggctttaa ataattattt ttccaaaagt agtcaaaaag ttttctattc
      541 ttggctggag ccatatggca ttgggggaca cactctattt ataaattatt ctctaaatca
      601 aaaaggttgt ttcaattgtc tttatacaga ttcaaactct aaaaataggt gttcaaatag
      661 agcttctctg gtaaaagaag gacaaatttt tgaaaggact ttagcgagtt gccgctcaaa
      721 atttatacca tataatgcta ttgcctcgac ggaatgtgca attaatgcaa taaaactttt
      781 ccataaagtc aatcaaaata atatgaccaa tagtactctt ttttcatgga taggagatgg
      841 gagtactttt gagaatttag gatatcaata tagcgacaga aatacttttc ttaatggagt
      901 caataagttt gaagaaagat tcttttctaa atattgcaaa gtatgtgggg agttagatga
      961 ttaaattttc gtgtgaccgt attgtcattg aagtaaggga tgaagttctt aagatatttg
     1021 aacaatatcg gcagggaaag gatgacttgc ctgaagctgg aggagttctt catggaaaaa
     1081 gacttaagaa tgggagcata ataattaccg atataacaac acctcaaata ggagatatac
     1141 aaaaaagatt actctttaaa aaaaaccgag aaattcatca acagctttct aatgagaaat
     1201 ggatagaatc aaataagttt tctattgcat tgggagaatg gcatactcat cctgaagctg
     1261 taccaactcc atcaaatgtt gataagaaaa gctggagact gaacgtgagc aagcaacatg
     1321 atgatagagt ttatgttttt attatcgttg gattttctga tttgagaatg tggttactgt
     1381 caaaaaaatc agatattagg caggctataa aactataaag gagtgattag gtgaaatcac
     1441 aaaagaatat ttggtggtgg gggtttttag taggaaaggt aatgatacca gcccttctct
     1501 taatttctct accgatgatt acttttttct ttaccaataa aaaaatgagt aaagaagccg
     1561 tgattttttt ctttggagac caaaaagcta cctttattga aacgttagta acatctcttc
     1621 agcttaattt gaattacatt ttttcaatta ttattgttat atcgt
//
LOCUS       DAFFSV010000234         1664 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.237, whole genome shotgun sequence.
ACCESSION   DAFFSV010000234 DAFFSV010000000
VERSION     DAFFSV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.237"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            131..505
                     /locus_tag="LR110_002931"
     CDS             131..505
                     /locus_tag="LR110_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL4823841.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="LR110_002932"
     CDS             complement(619..1248)
                     /locus_tag="LR110_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL4823842.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="LR110_002933"
     tRNA            complement(1382..1454)
                     /locus_tag="LR110_002933"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAFFSV010000235         1634 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.238, whole genome shotgun sequence.
ACCESSION   DAFFSV010000235 DAFFSV010000000
VERSION     DAFFSV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.238"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..144)
                     /locus_tag="LR110_002934"
     CDS             complement(<1..144)
                     /locus_tag="LR110_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823843.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDR"
     gene            382..996
                     /locus_tag="LR110_002935"
     CDS             382..996
                     /locus_tag="LR110_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4823844.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1161..>1634)
                     /locus_tag="LR110_002936"
     CDS             complement(1161..>1634)
                     /locus_tag="LR110_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823845.1"
                     /translation="LIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRNK
                     LVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQI
                     TLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          562 a          272 c          247 g          553 t
ORIGIN      
        1 cctatctaaa ggagttaatt cttggttatt atctactaca gtagttggtt catttgtact
       61 atcagcaaat gtaggaattg tagttccagt tacactaatt ccaatagtta aaatacctaa
      121 agataaaata aacttatttt tcattgtcta tcacctcgaa atcaaaaata agaaaacgtt
      181 ttcttaataa atcttaacag ataaatcaag ttatatacta gaaaattcat aaaatatatt
      241 tttaatgtca taatcgttta aattttcttg attgacattt atttacaaaa aatattgcca
      301 tttaaaaaga aaagtgacat atggcttcct cgtgtatgat tagggtgtat tttaaatagt
      361 tgcagaaagt aggtaaaagt tttgagagtt gcatacatac gtgtctcatc gatcgatcaa
      421 aatgaacaac gccagattga agaaatgaaa aaattcggtg cagagagaat tttcatcgag
      481 aaacaatctg gcgcaacgat tactcatcga cctgttttcc aagaagcatt agattacgtc
      541 cgagatcaag atatttttat tgtggaagct attgatcgac ttggaagaaa ttatgacgag
      601 atcatcgctt cagtcaatta cttgaagaag aaaaatgtga agctgatcat tactagttta
      661 ccgatcatgg ctgaagcaat tggcaatcct ctcctggata aattcattaa ggatctgatc
      721 atccaaattt tggccatgat cgctgaacaa gaacggactg aatcgaaacg tcgccaggca
      781 caaggaatta agatcgcaaa agcaaacggc gtctacaaag ggcggcccaa attatacagt
      841 gcagacgcaa aagatcccca acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa
      901 aatggtgttg cgattgctaa gattgctaaa gactacaatg ttacgagaca aaccgtgtat
      961 agaataaaaa aagacagcat ggtcaatgac aaatagttga tcatgctgct tttattatct
     1021 tctcatcttt aatttcctac atataattac tatctataaa attaagtctt actcagaaca
     1081 gataaagcat aaaattccaa caaaccattt tttacttaac taaataaaga gtctgttaat
     1141 ataatcttca cttatttacg ttaatcccct actgtatttg ttggttcctt atctatattt
     1201 tctacaaaat ccttattatc ttgtgattta gcttcgtata attctttgta tgtcgacaca
     1261 ttggttatac taattcctat agctagttct tgagactcta cttttctctc tagctccttt
     1321 gacaaagtga tttgagattt atttggttcc gttggagtca attctataga ttcttcataa
     1381 aaatctctat acataaagct atcattaatt ttataaaaaa aattaaatgt ttttagcact
     1441 gccatataac attcaaaaca ttcttctata gttggtttac ttccttgagg agaatgaacc
     1501 aatttattac gtttttctat gaaattttta atttcctttc ttatttcttt cggaaactca
     1561 atattaaagt accctttaaa catgctccct aataatttgt ctacaggggg agattgtaaa
     1621 ttctcaaata ttag
//
LOCUS       DAFFSV010000236         1630 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.239, whole genome shotgun sequence.
ACCESSION   DAFFSV010000236 DAFFSV010000000
VERSION     DAFFSV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.239"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..552
                     /locus_tag="LR110_002937"
     CDS             <1..552
                     /locus_tag="LR110_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823846.1"
                     /translation="IKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGY
                     GIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSST
                     LLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRL
                     SEEKILLPENWAELRKLVEYGII"
     gene            539..>1630
                     /locus_tag="LR110_002938"
     CDS             539..>1630
                     /locus_tag="LR110_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBL4823847.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIG"
BASE COUNT          537 a          210 c          261 g          622 t
ORIGIN      
        1 ataaaaaaaa ttcccaaaat agatgaaaac agtaaattga tgaaaaacat acaatcaatc
       61 tttgggatag aagaagtttt tgtcggagat aaatgtatct tttttgatca acctttatta
      121 tctgatggtt atggcataga tgaaacagat attttcgcta aagtaaatag tatattttct
      181 ggaaaaaaag aaactttaat taagcttcat cctagagcaa ggacaaatcg ttatggtgaa
      241 aaaaacatat tgaattccag tttaccgttt gaagtcttgc tacttgaaaa tgacttctct
      301 tcaacacttt tgattagtcc tatatctaca atttcttttt caccatctat gatgtttgaa
      361 aagacgatca actctattat gttggctaaa ttaattttaa atgaatatac agcattatct
      421 gaagataaaa aagaaacatt gacaaatatt gttcttttct gtgaacatta taaccgtcta
      481 tcagaagaaa aaattttact cccagaaaac tgggcagaat taagaaagtt ggtagagtat
      541 gggattattt aacacacttt ccaagtcaaa attagttaag tccagtttct ggtatactat
      601 agggaatttt tttattaagg gaatttcatt cataactatt cctatttttg taaatattac
      661 tactcaagca gaatatggtt tgattaataa tttcacttca tttgtatcaa ttttttcgat
      721 tcttgttggg ctaagcctaa attcggcaat taataatgca agttttgact ataaagatga
      781 cataaatgaa tttatgtcat cggtactttc tctagctaca ctttcgttaa ttgtgttctt
      841 attaataggg aacatatatt ttttgttttc aaatgctttt tttgatatga accaacttgt
      901 atttaatttg atgttattac aaagttttgc tagttttgta attagttata tccagtctta
      961 ttttacgatt aactttaacc attttaaata tttgatttta tcttttatat ctactttttt
     1021 gaatattgca atctctgtag ctttgatgca gacattatat tttgatgata aatatgttgg
     1081 cagagtagtc ggcggaacac tgtcatttgt gttgataggt ttagtaattt atttaagaat
     1141 aatgataaag ggtgctcatt tttttgataa aaaacagtgg agatacgctt taaaaatctc
     1201 tttgccgcta atcccacatt ccttagcaaa tatagtttta gcacaattcg atagaattat
     1261 gattaattct tatttaggtc ctgctgcggg gggaatatat tcctacatta gtaacatagg
     1321 tattattcta agcgtaatat ggatgtctac taataacgcc tgggttccat ggttctatgg
     1381 tgagatgagt aaaaataatg aaaaaaaagt gaagaaagtt agtaactatt atttagtagt
     1441 ctttactttg attacgatga ttgtaatggt agtttcgatt gatttagcaa aatttatggc
     1501 tccgaaagac tattattctg ggttatcttt agtggttccg ataaccttag gctatttctt
     1561 tcagttttta tatagtttac cagttaatgc tgaattttat gagaaaaaaa catcgtttat
     1621 tgcgattggt
//
LOCUS       DAFFSV010000237         1566 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.240, whole genome shotgun sequence.
ACCESSION   DAFFSV010000237 DAFFSV010000000
VERSION     DAFFSV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.240"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            575..907
                     /locus_tag="LR110_002939"
     CDS             575..907
                     /locus_tag="LR110_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL4823848.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
BASE COUNT          542 a          227 c          324 g          473 t
ORIGIN      
        1 cgaagagaga taatttcatt atttaaacaa gctaaaaatc ttgatattac ttcttttcag
       61 gcaatacgaa tattattcgc ttacattttt tgtataaatt taattgtctg ctatgcttgt
      121 gatgaattga ttttcgttct gcacttttgg atgataaatt tggacaattg aaaatacaaa
      181 aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt cgtcagcgtt catacacggc
      241 attaagattt ggcccttaat gcgttaacct ttcagtgtta cggtattacc cgcaatggat
      301 ttagtataac agatattcaa aagtgatacc actttttttg gctgtgttaa ggttaaaaat
      361 acgtgatatt acgatgaatc tatgcctaag cacaatgtta taaaaatgtc cgtgtggctc
      421 tttgccgtat gacgaacgct gaaattagag ggaacgcatg tttggatgcg ttccctcttt
      481 tttgtttgtc atgaacgaaa atcaattctc tatttttgaa aaataacaag aaaaacttgt
      541 taaaaatatt taaagaaatt gggtggttta ttttatgtca tatacaggaa ttttgagtct
      601 caaagatatt tgtcactacg gtaaacggtg taccgctaca gagaaaatca cgaagaaact
      661 atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc agaaagacaa
      721 agtctcagag gaaatgattt attacattgg gaaacaaaag caaataattt taaaagatcc
      781 tatcccctta aaagagctat atccaacaat caaacatgtt tatgaccaaa acggtgtact
      841 gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg ggcgtttgat
      901 cggctgaatt gatcattctc ataacttgta ttttatcgtt tttcgcttgc ttgaaaatat
      961 tgtcgagact tctaaaggaa cttagagtca ctagaaaaat aaggagtaga cttgatctat
     1021 caacaattcc cttcggggag tgacggggta ggttttaacg attggtaacg taaaaaattg
     1081 gtcaatcgag taaatgcgct tgtttggata gtggtgaaat gaatttgtgt aaataataag
     1141 cgcagagtaa acaagaggga atttagaagt tagacaagtt agaatagcgt ctagattaat
     1201 catgagcagc tactaaggga tctagacttt gacgcaaact catggaataa ggagtaggtc
     1261 ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac atgtagatac
     1321 ttgcgaggac gttgattatt ggatcaatcg agcatttacc gctgtctact agggtttctc
     1381 gtttcggacg ccataaacga gtcgaaattt tttatgtatt tttaagtaaa cgaaaaacga
     1441 taaaatatga acaagtcctg aataaagtga aaaaattgat ataaaacata gagaaaatga
     1501 aatttacgta atggaggaat gacaaatgaa aaattatgat ccgaatatac gattgggcac
     1561 acatac
//
LOCUS       DAFFSV010000238         1546 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFFSV010000238 DAFFSV010000000
VERSION     DAFFSV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.241"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            249..1199
                     /locus_tag="LR110_002940"
     CDS             249..1199
                     /locus_tag="LR110_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL4823849.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
BASE COUNT          566 a          225 c          248 g          507 t
ORIGIN      
        1 attagagagt atacaaattt aggtgtttta caagccaaaa aagtatatga aaaaaaaaaa
       61 aagggtctat aattacaaat tagataatac attagaagct ggagacaaga ttgtctagat
      121 aatgtgtgag ataaaatttt tggcaggtta tcatacataa gacaaaatgt ttttaaaact
      181 gataacgtat ttattccaca acaattacgc atttatttct gagaaaaata taaagcagga
      241 ggattacaat gctgaaaact tattccttaa caaaagatac attattagtt tcgtcagaat
      301 caaatatcaa aaattcttct gttgtttatg tttatggtga cgaacgagaa tatatcgaat
      361 cttctgaaca taaatatgac tttaaaatag gcaatatact aacgtttgat gatcgagttg
      421 catatgacac gatgatagat aagaacgatg aaaaaagttt gctcgtaagt tttttgtttc
      481 ccgaggtaca tgaaggtggg catctagata ccctgacgac atctgttgtt ttcttggtat
      541 ttaaaaataa gttgataatt tttacaaaac aaaaattaaa attcattccc gaactcttat
      601 ccaatatggt tttacgtgat gtcaatcatt tcgtggagga agtactttta aagatcatgc
      661 aaaaagattt ctatatgatg cttaacgacc ttcgtggagt caaggaaaaa attgatgatt
      721 tagactcaga aatatcaacg tccggttcac tcaaaccaac gtttggtgat ttattgactc
      781 ttcaaaaata catgatagct ttgtcaacaa cttataaagc taaccataaa gcgcttgatt
      841 tcattaaaaa aaatttcaca actattgacg atattgattt tagtactaaa aaaataattg
      901 atgatttata cgatacaagt gatacaatgg atagaatcat tttaagttac agccaatatt
      961 tggacaaatt ggaaaatatg ataaacaata tgatttcgta tcagctaaac atgattatga
     1021 aaaccctaac cgagatatca attgttttga ctattccagc aattattttt ggcttttggg
     1081 gaatcaatgt ccatgtcccg tttgaaaagt cttcttatgg tgttttagcc gtattgatta
     1141 tctctgctat actaagtcta atttgctggc ttttgatgcg acgcaaaaca tatttgtaaa
     1201 gtatttgtga taccctaata tgatttaaag cagaatttct ttgttgactt tttaaaaggc
     1261 tgattccttt caataattta atagcttcag cggctgctca gaagtcaata aattggacta
     1321 tttttattca gttacttagg caacatttga gatacgattg cgattataaa aattgatata
     1381 ccaaccaatg gctgcttgga agactgacat attagcctcg atatcatcga ataactaaat
     1441 acagaaaaat acaataaata ttatcaaact agggactata ctagaaaaca tcagtacatt
     1501 ccttaactca gggtctataa ttagaaaatc gcccctttga aaactg
//
LOCUS       DAFFSV010000239         1537 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFFSV010000239 DAFFSV010000000
VERSION     DAFFSV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.242"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            226..>1537
                     /locus_tag="LR110_002941"
     CDS             226..>1537
                     /locus_tag="LR110_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823850.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIE"
BASE COUNT          567 a          212 c          273 g          485 t
ORIGIN      
        1 caaatggcgc aaatcttgta cattttaaca caataaaaca aaaaatatag aatatttcct
       61 cataaatttt tccatttttt gcaatattct caaaaaaaag ataaaaaatg agaataactg
      121 atgaaaaaac aagcataatc catcataaaa tcagtatagg tgaaaagatg aactttcatc
      181 tgcatatcaa gtaattggta tgaggcattt tttgaggtgt aggatatgaa gttggattac
      241 cttatagaag aggaagatag agaaaaactg ctgctagcta gatatttaga atttgagaaa
      301 aagaattatg tagtgctgcc taagttatat gaaacattag gtttgtctaa atttaaaaca
      361 agaaattata tcatagaatt aaataaagaa tttactgaat tcaaaacaaa gccagagatc
      421 caaatttcag aagaagcaga gatccatgtc gaacgtctga atttgacgga tatcaaaaat
      481 ttaagaagaa tctactttca acgatcaaaa attgctcgac tgttaacgga gatcgtggat
      541 tcaaaagtaa cgatcgaaaa atttgcagag aatcattttt taagcatatc aagagcctat
      601 gtaaaaagaa gagaattagt ctcttttctt gaacagcatc aaataaagtt aaagaaaaac
      661 atattagttg gggaagaaat aaacatacgg aacgtattat ttggggtata tttcggggtc
      721 tttaatggtt tttctatgcc atttagagaa agcgtcatga atgaagcaaa gagtatcgtg
      781 caatattttg aatatctgtt caatttaaaa ctgtctgcaa caaaacgaat caaattcaat
      841 ttgctaacca ctattagttt gttgagggcc tatgggaatc aaccaatcgg aaaacgtttc
      901 ttttcagatg aatactttaa gagctcagaa ggaatgaaga tcatcaagga tttatcacat
      961 atttcttttc ttcatatccc taaagagcag atcattgatg agatttctta tatcatgttg
     1021 tttttgatca cggaagaaga atttgaatta gatggcgcat ttggacttgc ggaagctcat
     1081 tttcatgata tccacatgat tagtcaaaaa atgtcagtgg aattgattga tcaattacca
     1141 ttttcgattg aaatctctga agaagaaaga ctatatatac gaaaagaact tcgtatgggt
     1201 atgatgcatg tcaatcgaaa aaattggata ttcgactttg agatgactag ctttacgtct
     1261 aaaaagcagc tacagttttt cttcgagacg tatccaatat tcagtacagc aatccaaaag
     1321 gtcatacaag agaataaacg ctatttcttg ggaaaagatg aacgtttcat gattcgctta
     1381 ttttatgatt atctcttttt attggtcggt atttgtccgg tatcatattt tgaagcaccg
     1441 attcatgtct gtattgattt ttcgcaagga tctgcttata ccgattacat tatttctcaa
     1501 gtgaaaggat ttaaaaatta caatattgtg attgaga
//
LOCUS       DAFFSV010000240         1528 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.243, whole genome shotgun sequence.
ACCESSION   DAFFSV010000240 DAFFSV010000000
VERSION     DAFFSV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.243"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..785
                     /locus_tag="LR110_002942"
     CDS             <1..785
                     /locus_tag="LR110_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL4823851.1"
                     /translation="SNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNY
                     SYIVKVNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKA
                     SADVEKELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKI
                     GMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRK
                     EFFKVSLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            851..>1528
                     /locus_tag="LR110_002943"
     CDS             851..>1528
                     /locus_tag="LR110_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4823852.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELR"
BASE COUNT          642 a          194 c          268 g          424 t
ORIGIN      
        1 tttctaacgt agattcaact agagaaaaaa ttataagaac ttttgataaa ataaacaagc
       61 tttttaaaaa cgatgaagca gaattcaaaa aagaattact tgaattaaaa cttcaagaat
      121 tagaactcaa ctattcatac atagttaaag ttaacgatga aaaagaacag caaaaagcaa
      181 taaaagaaca aatgattgaa gaagaaaagg taagacgtga aatagaacgt gaaaagaaac
      241 gcattgataa agaagaacga cagtttaact cagagataac taagttaatg gcatacatgc
      301 aaaaagctag tgctgatgtt gaaaaagaac tctatgctga taaaattaaa gaactggaag
      361 agaaattaag agaactagaa actgtcaaag aagatgtatt gcaaagagaa cttaatacta
      421 gagctggcta tgtttatgtc atcagtaata tcggatcttt cggtgaagat gtctataaaa
      481 taggaatgac tagacggtta gaacctatgg atagaattaa ggaacttagt agcgcttctg
      541 taccttttga atttgatgtt catgcaatga tattttcaga agatgcacca acattagaaa
      601 atcaactaca ttcgcatttt agaaaaaacg aagtcaataa agttaatcaa cgaaaagaat
      661 tctttaaagt atctcttgat gaaattgaaa aagttgtttt agaaaattat aacggaactg
      721 tatcttttac taaactagct caagctgaac aatacagaag atcattagaa ttaacagatg
      781 tgtagtaaaa agattagcct tcgggctttt cttttacaac gataagaaca tacattcgaa
      841 aggagataac atgaaacgag tagcattata tatgagagta tcaactgaac aacaagccaa
      901 acatggagat agtcttagag aacaaaaaga aaccctctat gaatatatcg aacaacacaa
      961 ggatttgaaa gtagttaatg aatatgtaga cggtggtatt tctggtcaaa aaataaatcg
     1021 tgatgaattt caaaaactat tacaagatgt aaaagaaaat aaaatagatc taattttatt
     1081 tacaaaatta gatagatggt ttagaaatct acgacattat ttaaacactc aagaaatttt
     1141 agaaaaacac aatgtttctt ggaatgctgt ttctcaacaa tattatgata caactacagc
     1201 gtatggcaga actttcattg cacaagtaat gagttttgct gaattggaag cccaaattga
     1261 ttcagaacgc atcaaagctg ttatggcaaa taaaattgca caaggtgaag tagtaagcgg
     1321 gaaaactccg ttaggctact ctatagaaaa taagaaatta gtaataaatg atgatgctcc
     1381 tatcgtgata gatatattca actactttct ttctagtggt agtttaagaa aaactgttta
     1441 ttatttaggc tctcagtatg gaatcgttag agattatcaa agtgttaaaa atatgttgac
     1501 gaacaaaaaa tacattggtg aattaaga
//
LOCUS       DAFFSV010000241         1527 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFFSV010000241 DAFFSV010000000
VERSION     DAFFSV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.244"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(137..652)
                     /locus_tag="LR110_002944"
     CDS             complement(137..652)
                     /locus_tag="LR110_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HBL4823853.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNGERLSF"
     gene            complement(834..1445)
                     /locus_tag="LR110_002945"
     CDS             complement(834..1445)
                     /locus_tag="LR110_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL4823854.1"
                     /translation="MIDIISAIMVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
BASE COUNT          529 a          303 c          196 g          499 t
ORIGIN      
        1 aatattagtt tttcgtgaat aaatatctat atctaaattt agttttatgt attcctcata
       61 gaaataaccg agcatttgct cggttattgt tcttcttatt ttccagtatc cttcttttct
      121 ccacagctca ttttaatcaa aagcttaatc tttccccatt tttgtctttc tcacttataa
      181 cgaaacactg ccgactgacc ggccattcaa tatctacgct ttcataactt atacatttgg
      241 cagcattaga attgaaatca ttatctacgc accactgaac tagcgtattg tcctccatag
      301 taagatagcc atgagcatat ccacgtggga tatacgcaca tttaccattg ctttcactca
      361 atgtgttgac atacacacat ccataagtgg aagatccttg tcgaatatca agtgcgatat
      421 tcataatttc accatgtagg cacatcacta tctttccttg agcatgctct ccactctgat
      481 aatgtagccc tcgcatagta tatttattat gactaaatcc ttgatttatt tgcttaatgt
      541 gaaactcaat accaatcgag aaaagagtct cattatccaa tgcaacagca gtatatcctc
      601 tgaaatccga aaacttaatt atatcaaaca cttttaccat aactaccacc atgttatata
      661 ttttaccaat tttttaattc gctcgacctc atttaaagca ataaaattcc ctacctcttg
      721 aagatataac aacaatgttt tagcaaaaat agatggcatg taaagtattc ttgatgagat
      781 tacacaatcc tgtctgcaga aaaatattaa ccatattaaa attcaaagca gttctatttt
      841 aagtctatat acttatttaa tcggtagaat agccaatata gcaaagcacc aattaagccc
      901 cctagggtgt tataaaacaa atccgatagt tgaaatgtac caagcctaaa aaacagttgt
      961 gtaaattcta tacttaatga gaataaaaac acatacttaa tcccaacata gatggtgcgt
     1021 ataatatcac atttaatgtc acgccaccat aacaataaca tgcaaaaagg gataaacagc
     1081 atcgtgttct ccacgcattc tgttgtcaat ctgctctctc ctgtagcatt atctataata
     1141 tataatgacc actgtccaat aacgtttgaa agcgggttca tccacatatt tcgattaagc
     1201 aaggttttaa ataatatcaa agtaaaataa aacgcaagaa aaaagagtct gcgaaattta
     1261 ctagatgctt taaaatatct caaccagatt aatacagaat cttttaagtt tttaggatct
     1321 cgagagttat caactataaa tagataccag tacataaaca gatacgctgt taatacggca
     1381 aataagaacg gctgatataa tgctgttaat acatttacca ttatagcaga aataatatct
     1441 atcattctct atctctctat gagcaaatgt ttatttgctc ataattctcc ttactaaaag
     1501 tagcaatcgt tttctaaata taattgc
//
LOCUS       DAFFSV010000242         1520 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.245, whole genome shotgun sequence.
ACCESSION   DAFFSV010000242 DAFFSV010000000
VERSION     DAFFSV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.245"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            10..1476
                     /locus_tag="LR110_002946"
     CDS             10..1476
                     /locus_tag="LR110_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBL4823855.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          321 g          493 t
ORIGIN      
        1 gtatagatta tggaaaaaca gcaagacaag ggcttcttcg ggcagccgaa gggattgtca
       61 actttgttct tcactgagat gtgggaacga tttagttatt acggtatgcg tgcattactt
      121 atctattata tgtacgatac agttgccaac gggggacttg gattaccacg agcaacagca
      181 ttagctatca tgtcgatcta cggatctttg atctacatgt ctagtatcgt cggcggatgg
      241 gtatctgacc gagtgttagg agcaaaacgc actgtattct tcggtggagt tttcattatg
      301 cttggccaca tcgtcttggc tttaccattt ggtgtaagta cattgtttgt ttcgatggca
      361 ttgattatta tcggaaccgc ctttttgaaa ccaaatgttt ctggcatggt cggacatctt
      421 tattcaaaga ctgacttgcg aagagatgca ggattttcga ttttttacat gggtgtcaac
      481 ttaggggcat tattagcacc actaatcgtt ggtacaatcg gtcaagaata taactatcat
      541 ttagggttct ctttagctgc tattgggatg ttctttggat tattacaata catcatccaa
      601 ggacgcaaga cattagacgg agtgggtaca acaccgccga atcctttgac aactgaagaa
      661 cgtaaaggat tcatccgtaa cttgatcatc gcacttgttg ttatcgctgc agtattcggc
      721 ggggcacaac taactggtca tctgacaatt gacttttttg tcaatacaat cagtgtattg
      781 gggattatct taccgttgta ttactttatc aaaatgctga cttcaaaaga tgtaactgct
      841 gaagaaaaac caagagtatg ggcttatatc ccattattcc ttgcagcaat cattttctgg
      901 tcattacaag aacaaggttc atctatctta gctttatttg ctagtgaacg tacacaatct
      961 acgctatttg gtgtaacgat ccaagcaagc tggtatcaat cattgaatcc aattttcatc
     1021 tttattctga caccagtatt cgtaacgatg tggacgaaac ttggtgaacg tcaaccatct
     1081 acagtagtga aattctctct tggactttta tttgcaggat tatcgtactt gctattgatg
     1141 ttgccgggta tgctttatgg tacaagtgga aaagtaagtc cattgtggtt gttgggaagt
     1201 ttcttcatcg tggttattgc tgaattgtgt ctttcaccag ttggtttgtc tgtgacaaca
     1261 aaattagcac cacgagcatt tgaatcacaa acaatcgcta tttggttctt agctgatgct
     1321 tcttcacaag caatcaatgc acaaattgca cgcttctaca caccgggaac agaatctgct
     1381 tacttcggta ttatcggtgc agtagcaatc gtcggtggta tcttgctatt tactgtgaaa
     1441 gaaccaatca aaaaactaat gggctctatt cattaatcaa tgaacataaa aacaggaatt
     1501 gcttatttgc agcagttcct
//
LOCUS       DAFFSV010000243         1517 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.246, whole genome shotgun sequence.
ACCESSION   DAFFSV010000243 DAFFSV010000000
VERSION     DAFFSV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.246"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(840..1358)
                     /locus_tag="LR110_002947"
     CDS             complement(840..1358)
                     /locus_tag="LR110_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBL4823856.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          468 a          297 c          212 g          540 t
ORIGIN      
        1 ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt
       61 gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga
      121 aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat aaaaactaat
      181 gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt
      241 caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa
      301 taggataggc gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta
      361 tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt
      421 gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga
      481 cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact
      541 atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag
      601 tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc
      661 tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag
      721 tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt
      781 tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt
      841 tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact
      901 gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc
      961 cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta
     1021 atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag
     1081 gtatttgtaa accacttata taactctcct aactgattcc atttaaattg atcagaaggc
     1141 gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa
     1201 ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac tcttttatcc
     1261 ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt cctatttatt
     1321 tcttttttta attcttcttt atttttatat gttctcatta tattacctcc ataaattcct
     1381 aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg ttctacaatg
     1441 catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa atatcccaac
     1501 gtctcatcta aagaaat
//
LOCUS       DAFFSV010000244         1453 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.247, whole genome shotgun sequence.
ACCESSION   DAFFSV010000244 DAFFSV010000000
VERSION     DAFFSV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.247"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            102..1397
                     /locus_tag="LR110_002948"
     CDS             102..1397
                     /locus_tag="LR110_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL4823857.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          459 a          286 c          271 g          437 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      361 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      421 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      481 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      541 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      601 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      661 gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct
      721 atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt
      781 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
      841 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      901 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      961 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
     1021 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
     1081 ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag
     1141 atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata
     1201 caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca
     1261 gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg
     1321 cgtacggctt tcgcaacttt tacaatttca aactacgaat tatgattcgt catggcaaaa
     1381 catttttaac caaataaaca gaacagaaag aggttttctt ctttctgttc tgtccaaatt
     1441 attctaagat ttc
//
LOCUS       DAFFSV010000245         1445 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.248, whole genome shotgun sequence.
ACCESSION   DAFFSV010000245 DAFFSV010000000
VERSION     DAFFSV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.248"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            8..418
                     /gene="spx"
                     /locus_tag="LR110_002949"
     CDS             8..418
                     /gene="spx"
                     /locus_tag="LR110_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL4823858.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            432..788
                     /locus_tag="LR110_002950"
     CDS             432..788
                     /locus_tag="LR110_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBL4823859.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          533 a          217 c          267 g          428 t
ORIGIN      
        1 tgagaaaatg ataaaagtat atacttcgcc tagctgtact tcctgtagaa aagcaagagc
       61 atggttgcaa gcgaatcaac ttgaatttga agagaagaat atttttgccg aaccgttaac
      121 agaaagtgag atcaaaaaga tcttggcagc gactgaagga ggagttcaag atattatttc
      181 cactcggtcg aaaatttacg aaaagctggg catagatttt aacgaattaa ctttaaagca
      241 aatgatcgtc ttattcaaag aatatccttc attattgaga cgacctattt tgattgatga
      301 aaaaagaata ctaatcggat ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat
      361 tcgatgtgtt gccagaaatc tgatctattt acaaaacaat aacatcttaa attcataggg
      421 agggagcctt cgtggaagtg aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg
      481 cgaaacaatg gttagaacac catcaattgc cttatcaatt tgttgactta gaagaacgcg
      541 atctaagtac agaagaagca gaagaattat gcagttttga agaagtgcaa gcagaacagc
      601 tatttgcgac ttggtctgag gcttttcaca acattgcgat tgacctttcc tgtgtaggaa
      661 aaaaacaact gattttttta tgcacacaac ataccaatct cctcagacgt cctttgatta
      721 ttatcgatga agttctattt attggataca atgagcagtt gatggaacag cttatcttaa
      781 ataaatagac tttaactgtt ttaatcaagt cactgagcat ttcatacagt tgtaacagtc
      841 gaacacttag catcaagcgt gttatgtgtt ctttttttga ctttattttt ctactataga
      901 gacatgctca atttatgtgg tcaaaattca aatctaggtg gggatataga gttaaaaata
      961 tattgacatg tgtaaattta catgatatat tcatttctgt tgttttaagt agcaaagcaa
     1021 taaaatagta ttttaaaaaa catataaaat agttgttgac atgttagaaa tgacatgata
     1081 agataactga gttgttaaaa aagaaccact tgtgaagctt gattctatcg aaaaaaaatt
     1141 caaaaaaagt tcttgacaaa taacaaatga taagttatga taaatgagtt gctgtaagat
     1201 ttcgaaaaac gaaatcaaaa aaacttttaa aaaagagttg acaattactt gtctctctga
     1261 tatgatataa aagttgctac ggcagccaaa aacgaatcgg ggaacactcc tgatgaagta
     1321 gacctttgaa aactgaacaa agtaagacaa accaaatgtg tagggcgtct tgattcaatt
     1381 caagacaaca aacatttttt aacaagcaag caatatgcta gcaaacaaat tgagcttaac
     1441 aatcg
//
LOCUS       DAFFSV010000246         1433 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.249, whole genome shotgun sequence.
ACCESSION   DAFFSV010000246 DAFFSV010000000
VERSION     DAFFSV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.249"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            23..>1433
                     /locus_tag="LR110_002951"
     CDS             23..>1433
                     /locus_tag="LR110_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBL4823860.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKI"
BASE COUNT          518 a          176 c          298 g          441 t
ORIGIN      
        1 taataagtag aggagaaaga aaatgactat aaaagtagat ttacgaaaaa aaccatataa
       61 tttagatgaa gatgctattt cttggattga agagacgatt gattctatga ctttggaaga
      121 aaaaataggt caattgtttg ttaatatggg atctagtcgt gataaaaatt acttgagtaa
      181 tatggtcaat cagtaccata ttggagctgt acgatataat ccaggaactg ctgatgaggt
      241 atatgatcaa aactatatct tacaaacaga aagtaaaatt cctctattaa ttgctgctaa
      301 tacggaagca ggtggtaacg gagcttgtac agatggtact gaagttggtg tagaagtaaa
      361 aattggtgca acaaatgatg cgaaatatgc ttatgaatta gggagagttt ctgggattga
      421 agcatcggct attggatgta actggagttt tgctcctatt gtagatatta atagaaattg
      481 gagaaatcct atcatttcta gtcgatcttt tggacaaaac cctgataaag ttttagaact
      541 tagtttagct tatatgaaag gtattcagga gagtggaatt ttgcctgcag ctaaacattt
      601 ccctggtgac ggtattgacg aacgagatca gcatctatct tttagtatta atagtttagg
      661 tacggaagaa tgggataatt cttttggaaa agtatactca ggcttaatag aagctggatt
      721 accttcaatt atggcaggtc atatccatat gccagcttat tcaaaacatt ttaatcctga
      781 tctgaaagat gaagatttac ttcctgcaac tttatcaaaa gaattaatca ctgacttact
      841 tagaggaaaa ctcggattta atggattagt agttactgat gctagtcata tgttaggaat
      901 gactggggca atgaaacgat cagaattatt gcccacttct attgcagctg gatgtgatct
      961 atttctattt tttaatgatc cagatgaaga ctttaattat atgatggaag gctatcgtaa
     1021 aggaattatc actgatgatc ggttattaga tgccttaaaa agaattttag ggttaaaagc
     1081 taaattggga ttacacaaaa aagcaaaaga acaaatttta ttacctaagc aagaagctct
     1141 ggaaaaaatt ggtttaattg aaaataaatc aatttttaaa gaggtggctg accaagcaat
     1201 tacattagtt aaagataaac aagatatttg gcctattgta ccagaaaaat ataaaaggat
     1261 tttactagta gatgtacatg gggtaaaagg tgggtttggt gaagtagttg ctggaaatag
     1321 acctcaagcc attgatttat tgaaacagat tttgtcagca aaaggatttg aagttgaaaa
     1381 atacatttca cctgaagaag aattgaaagg attatcgcaa gaagaaaaaa tta
//
LOCUS       DAFFSV010000247         1403 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.250, whole genome shotgun sequence.
ACCESSION   DAFFSV010000247 DAFFSV010000000
VERSION     DAFFSV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.250"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(132..539)
                     /locus_tag="LR110_002952"
     CDS             complement(132..539)
                     /locus_tag="LR110_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823861.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(558..1376)
                     /locus_tag="LR110_002953"
     CDS             complement(558..1376)
                     /locus_tag="LR110_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL4823862.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          405 a          234 c          193 g          571 t
ORIGIN      
        1 aatgttgatt taatcgcaaa tttacacgtt gtttattaag ttatacctta aatatttaat
       61 gtataactta tactttaata ttcatttatc tagtataact tatactttaa ttgaataccg
      121 catgaaaaaa attattttgg taatttttca aactcttctt gtatttcttc tcttatgtgt
      181 tcttcttttg ctactcctag tctatgctgg atatatgagt ctgctaatgt attaacaatt
      241 ttttcgaaag aaattctccc tttttcactt tgtaactccg tcatatagtc ttgtaaaatt
      301 gagagtttgc tatttaatgc cggagagatc tttttaggaa ctgatacctt gtttctttta
      361 tcaggaattg aaactggtcg gcctcttttt ttcaatgtaa tatttcttgt gttactcaat
      421 gcactattgc taaagttata ttcaccgctt attttcacat cttggcgttt gttattgggt
      481 aataatggct caaattcacc attctccatc attttaggaa tatcaaaatc tttaggcatt
      541 acttatgcaa ctccttatta tttatttaaa aactcttgtc tagcatctag ttctgccaaa
      601 atatttataa atacttggtg tgctttataa tcccattgat caaaatttcc atttttgtat
      661 tttcttaaat gtataccaat tttagaatac cttctcaatc ttttttgata atgaatgaga
      721 tttttggata ccacggttcc gtatttttca attgcttctt ctaggatttc ttcatcaaaa
      781 tcatcatttg gttctaccat acaaggtaca atatctataa tttgcaaatc aagatcgtat
      841 ctatcgacca tgaatttttg ataatttaca tatttgttga ctccctctaa ggattcttca
      901 gctgtttgaa atgaaattat cgaatagtct gaagctgcca tagcattatc ggaaaattcg
      961 ctaatggttg gtggtacatc aataaatatt tcttgataat tgttctttat tggaagaagt
     1021 aattttttca agacagtaac ttggtctaat tcattttcat gtttattagc aaaagcggag
     1081 aatgatcgaa aactagtgtc acatgccaga aggtcaagat tttcatttac cttaataata
     1141 ctacttgcta attctccatt tgcgacacca tttgttatag tggttttgat atcggttata
     1201 ccgaaagttc ttgctatgac ttgtgttaag ttcgcttgag ggtctaaatc aataataagc
     1261 gtttttctat ttcttatcat tgcattgtca taagcaagca tgctgactat cttagtttta
     1321 cctactcctc ctttaaaatt gccaactgta aatgtagtcc cttgaaaagt atccatgtaa
     1381 atcctcccaa tctttaattg ata
//
LOCUS       DAFFSV010000248         1389 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.251, whole genome shotgun sequence.
ACCESSION   DAFFSV010000248 DAFFSV010000000
VERSION     DAFFSV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.251"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            278..820
                     /locus_tag="LR110_002954"
     CDS             278..820
                     /locus_tag="LR110_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823863.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1326
                     /locus_tag="LR110_002955"
                     /pseudo
     CDS             976..1326
                     /locus_tag="LR110_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          551 a          164 c          264 g          410 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc ttgatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggct
//
LOCUS       DAFFSV010000249         1384 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.252, whole genome shotgun sequence.
ACCESSION   DAFFSV010000249 DAFFSV010000000
VERSION     DAFFSV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.252"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..282
                     /locus_tag="LR110_002956"
     CDS             <1..282
                     /locus_tag="LR110_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL4823864.1"
                     /translation="VAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFISATVGA
                     CSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERKL"
     gene            282..1229
                     /locus_tag="LR110_002957"
     CDS             282..1229
                     /locus_tag="LR110_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL4823865.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1244..>1384
                     /locus_tag="LR110_002958"
     CDS             1244..>1384
                     /locus_tag="LR110_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBL4823866.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLT"
BASE COUNT          534 a          185 c          265 g          400 t
ORIGIN      
        1 gtagcagtaa cagctatgca aagtgtggga actatattaa ttgttgcaat gttaattaca
       61 ccagctgcta cagcatattt atatgcaaac agtttaaaaa ctatgatatt tatttcggcg
      121 acagtcggag cgtgctcatc attgctaggt ttgttcattg gttatagttt taacatagca
      181 gcaggatcaa gtattgtttt aacagcggca ttcttatttg taattagttt tctaatctca
      241 ccaaaacaaa aatttttaag aaagaaggag agaaagttat gatgagaaaa tggaaagcag
      301 tactgggaag tctggggatt ttgattgctc tttttatatt cggggcatgt tcaacaaata
      361 gtaaagacaa agatacagtg gcttcgaacg aaaaattaaa ggtagtagtt actaattcga
      421 ttttagcaga tattactgaa aatatagcaa aagataaaat tgatttacac agtatcgtac
      481 ctattgggaa agatccccac gaatatgaac ctttgcctga agatgttcaa aaaacttcaa
      541 aagcagattt gattttttat aacggtgtta acttggaaac tggaggaaat gcttggttta
      601 caaaattagt aaaaaatgcg aacaaagagg aaaacaaaga ctattttgca gcaagtgatg
      661 gcatagatgt tatttactta gagggtcaga gtgagaaagg gaaggaagat ccccatgctt
      721 ggttaaattt agaaaacggt attatttacg ctaaaaatat tgaaaaacag ttagcggaaa
      781 aagatcctga taataaaaaa ttctataaag aaaatctaga taagtatatt gaaaaattgg
      841 attctctaga caaagaagct aaatctaaat ttgcttcaat tccgaatgat aaaaaaatga
      901 ttgttacaag tgaaggatgc tttaaatatt tctcgaaagc gtataatgtg ccttctgctt
      961 acatttggga aatcaacact gaagaagaag gaacaccaga tcaaataaaa cacttagttg
     1021 aaaaattacg cacaacaaaa gttccctcct tattcgtaga aagcagtgtg gacgatagac
     1081 caatgaaaac agtatcaaaa gataccaata ttcctatcta ttcaacgatt tttactgatt
     1141 caattgcaga aaaaggacaa gatggtgata gttactatgc gatgatgaaa tggaacctgg
     1201 ataaaattgc tgaaggcctt tcgaaataat ttggagggta ggtatggtac aaattaaaac
     1261 aactactata ctaatttatt tactagcaat tcaggaatta tcaaaaagaa aaaagaaatt
     1321 aaaaaataat gatttagcaa aagttctaag tgttagtcca gcatctgtaa gtgagatgct
     1381 gact
//
LOCUS       DAFFSV010000250         1376 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.253, whole genome shotgun sequence.
ACCESSION   DAFFSV010000250 DAFFSV010000000
VERSION     DAFFSV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.253"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..326
                     /locus_tag="LR110_002959"
     CDS             <1..326
                     /locus_tag="LR110_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823867.1"
                     /translation="LYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNE
                     NFTGNISMSGYSQELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFI
                     NSTLT"
     gene            625..>1376
                     /locus_tag="LR110_002960"
     CDS             625..>1376
                     /locus_tag="LR110_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL4823868.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKIS"
BASE COUNT          538 a          212 c          234 g          392 t
ORIGIN      
        1 atctttataa gaattgttta cttcgaatta acagcgcttc atgggataac gaggagtatt
       61 atcttcttgt actgaatcaa ttaacatcga ttgagattca attgttagca tgcttaatga
      121 gtcgtggaaa cgaaaatttc actggaaata tcagcatgtc tggctattca caagagctaa
      181 tagatggtag cttaaataga ttatctgatt ttggtctgtt agaaaaaacg attagctcca
      241 tagtattagg tgatgtcggc aaacaaaata tgggctacaa aatatctcat ttaggaattc
      301 aatttattaa ctcaactctt acttaagttt tctttctaat atgacatcta gacctatcag
      361 caatgcaaca ataaaaaacc agacatacca ataatcaaca aataatctaa actctggaag
      421 ttcagttatt cttttaaaaa tcactttaat cataataaac acctctgatt ggatgatcta
      481 atacacatcc aattatacca ttaaaacgac aaatagcaca tccttgcgcc ggcaagcaaa
      541 aaggatgtgc aaaaaataat aaaacccaaa caatgggctt ctttatagtt cctattgtat
      601 cagagaaaga gagctgattc aattatggca agattagtca aacgtggaaa tagttggcaa
      661 tacgaaattt catacaaaaa agatgacgga aaatacacga agataagaaa atcaggattt
      721 aagacaaaag gagaagcaaa agatgccgcc aacgaattag aatataactt gaacaaaggc
      781 cttaaagggg atcgcaaaaa tctattatta tcagattact ttgaggattg gatgcaactt
      841 tataaagaag gaacagtatc tcctatcact tatagaaaat acgaagatac gttaatgaac
      901 ataaagaaat atatgccagc ggtattgatt tctgatttag atagagtggg atatcaacgc
      961 tttttaaata agtatgctaa agaccatgta aaatctaccg ttattaagtt taataaccat
     1021 attagagcgt cgttgaaaga tgccgtagaa gaaggattaa ttccgtttga tccaactaga
     1081 aaagcagtaa tcaaaggaaa agattcattg aagccaaaag aagataaata tttagattat
     1141 gatcaattta aatctttaat gaaactcgta gaagaaaacc tttctgcgca gtactcttct
     1201 cctatgctcg tgttagttgc tggtgccact ggaatgcgat ttgctgaact tctaggatta
     1261 acatgggaag atatcgattt cgaagatcaa atcatcacta ttaataaaac atggaattat
     1321 aaattaaatg aatggggaaa aacaaaaaac gaaacttcaa ataggaaaat ttccat
//
LOCUS       DAFFSV010000251         1372 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.254, whole genome shotgun sequence.
ACCESSION   DAFFSV010000251 DAFFSV010000000
VERSION     DAFFSV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.254"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            37..1338
                     /locus_tag="LR110_002961"
     CDS             37..1338
                     /locus_tag="LR110_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL4823869.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          446 a          275 c          253 g          398 t
ORIGIN      
        1 ttgaatcacc acaacacaat caaaggagcg ttctgtatgc ctgatcttca tcatatcaaa
       61 ctactacttg gaatcaaaga taaaaacatt ttcatcacaa atgtggaatc gaagagcatt
      121 aagaatatca agagtcttgt tgtttctgcg acgttaagta aagaaattcg tcgctgtcct
      181 ttgtgtaaac agatgaatca tgaaggcatg atcgttaaga acggaaagaa aaaatcgctc
      241 attcaactta ataaatgtgc gaaccaactg acttatcttg ctttagcgaa acaacgctac
      301 cactgtcgtg gctgccacac ctattttacg gcgaatacct acattgtcga tagaaactgc
      361 tttatcgcta aacaagtgcg ctataaaatt ttagaagagc taactgaaaa acaagcaatg
      421 acgacaattg ctaagcattg cggcgtttct tggtcgaccg tttctcggac actcgcttct
      481 ctgatcccca tgacaaaagt aaaaagaaat tggttaccgc gttgtttatt aatggatgaa
      541 tttcgttcac tgaaaaatca agtcggtccc tactcctttt cttgtatgga tggcgatact
      601 ggaaaattac tggatattct tccttcacgg aagaaaaagg ctctcgtttc ttattttatg
      661 caatttgaac gacgcgcacg attgaaggta aaggtcatcg tgacggacat gaatgtttct
      721 tatgcctctc tgataaagga atgttttcca aaggcaaaac tcgttgtgga tcgttttcac
      781 attgtcaaac atctcattcg taaatttgag gatattcgcg tacgaatcat gaaaagcttt
      841 gatcgaaacg atccaacaca agccaaatat tatcgacaat taaaggcgtt gtctcggctt
      901 ctgatcaaac gacaggacaa gcttgtttat gacaaatgga ccaaatggcg caattttggt
      961 tgggcgtatc tcaccgaaag tgaagtggtg gatcgtctgc tttcgatctc tgacgaatta
     1021 cgaatcgcct atttttacta tcaagaaatt cttcaagcgt ttcacgacaa agaggcggac
     1081 actttcttta aacttgtaag aacgatgccc aattccgttc caaaagaact gcattctata
     1141 aaaaaagctt ttataaacta tgaatccggc attcgtttag ccctcgaatt gccttattca
     1201 aatgccaaaa tagaaaatct gcatacccac atcaaagcat tgaaacgagt cgcttatggc
     1261 tttcgaagct ttcggaaaat gaagaccaga attttcttac taaataatct gattacctat
     1321 gaatcaaaaa acatctaaag tagaaaagga attctacctt agatgttctt at
//
LOCUS       DAFFSV010000252         1362 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.255, whole genome shotgun sequence.
ACCESSION   DAFFSV010000252 DAFFSV010000000
VERSION     DAFFSV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.255"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            82..>1362
                     /locus_tag="LR110_002962"
     CDS             82..>1362
                     /locus_tag="LR110_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBL4823870.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAFFSV010000253         1362 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.256, whole genome shotgun sequence.
ACCESSION   DAFFSV010000253 DAFFSV010000000
VERSION     DAFFSV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.256"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            82..>1362
                     /locus_tag="LR110_002963"
     CDS             82..>1362
                     /locus_tag="LR110_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HBL4823871.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          462 a          255 c          259 g          386 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAFFSV010000254         1357 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.257, whole genome shotgun sequence.
ACCESSION   DAFFSV010000254 DAFFSV010000000
VERSION     DAFFSV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.257"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..664
                     /locus_tag="LR110_002964"
     CDS             <1..664
                     /locus_tag="LR110_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HBL4823872.1"
                     /translation="QGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDC
                     FIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGA
                     KVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEV
                     SRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEED
                     VE"
     gene            661..>1357
                     /gene="neuC"
                     /locus_tag="LR110_002965"
     CDS             661..>1357
                     /gene="neuC"
                     /locus_tag="LR110_002965"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBL4823873.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQ"
BASE COUNT          472 a          177 c          280 g          428 t
ORIGIN      
        1 acaaggattt aaaattggct caggagattt aacaaattta ccttttctta gaaaaataga
       61 tatgtatggg ttgccagttt tattatctac aggtatggct gatattcatg aggtcagtga
      121 ggctgtagat tgtttcatag atagtccagt tactatttta cattgtactt caaattatcc
      181 ggctagttct gaagatatta atttattggc gattaacaca ttaaaagaaa tgttcgggat
      241 acccgttggg tactccgatc attcattggg atatgatgtt ggtatttgtg ctgttgcgat
      301 gggtgctaag gtaattgaga aacattttac cttggataag gaactacctg gtccagatca
      361 taaagcttct ttagatcctc aggaatttgc agattttgta aagcatatta gaaacactga
      421 aatattttta ggtgatggtg ttaagcgagc aatgccttct gaacttagta caaaagaagt
      481 atcaagaaaa agcctagtgg tcactgagaa tttaaatgtg ggagatattc ttacagagaa
      541 atctcttaca ataaaacgtc ctggaaatgg aatagctcct aagtttttat atcaatatat
      601 tggcaaaaaa gttaaaagac caataagtgc agatagtgtt ctgcttgagg aagatgttga
      661 atgaaaaaaa tacttatagt aagtggaaca cgagctgatt atggaatata taaacctgtt
      721 gctgagaatt tactacaaga taattgggat gtatcatttg ttgtttctgg aatgcattta
      781 tcccataaat atggatttac taaagaattg atcttagctg acggatttag aattagtgga
      841 gaagttcaaa gtttgttttt agagaacaat aagggaaaca tggctcggtc tgtggcatta
      901 gagataaatg gtttcacaaa tatatttgaa caaactagcc ctgattttgt attattacta
      961 ggggatagag gagaaatgtt ggcagctgcg atagcttgtt tgtatcttgg aatcaaaaca
     1021 gcacatattc acggaggcga agtgtctggt actgtagatg aatcaataag acatgccata
     1081 acgaaaatag catctgttca ttttgctgct acggaaaaaa gcaaagagcg tttaataaaa
     1141 atgggggaag attcttggag agtttttccc gtcggagctc ctagaataga tactatttta
     1201 aataatagat tacctagttt tagagatata aaagaaaaat atcatctaaa atttgaaaag
     1261 aaaaaatacg ttttgcaagt atttcatcca gttgtaaccg aactagaaaa tatagaaaaa
     1321 caaattttag ctttgatcaa ttcaatggaa caacaat
//
LOCUS       DAFFSV010000255         1348 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.259, whole genome shotgun sequence.
ACCESSION   DAFFSV010000255 DAFFSV010000000
VERSION     DAFFSV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.259"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..309
                     /locus_tag="LR110_002966"
     CDS             <1..309
                     /locus_tag="LR110_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBL4823874.1"
                     /translation="PYFNMVKRIENEVTIVNKSRYTSRQQTPEVFEMNASMYSFKPEF
                     LKKSNYIFDGQCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR
                     "
     gene            314..1261
                     /locus_tag="LR110_002967"
     CDS             314..1261
                     /locus_tag="LR110_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBL4823875.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          451 a          192 c          275 g          430 t
ORIGIN      
        1 ccctatttta atatggtaaa aagaattgaa aatgaagtta ctatagttaa taaatcaaga
       61 tacacatcta ggcaacagac acctgaagtt tttgaaatga atgcctcaat gtattctttt
      121 aaacctgaat ttctaaaaaa aagtaattat atttttgatg ggcaatgtgg gataataaaa
      181 atgagagatt atctaatttt agatattgat agtgaagaag attttaaatg gttagaattt
      241 ttgtttccta aatttttaga agattcttcg gagcttcaag atatctataa ttatttatat
      301 cggaggtaaa aggatgagct gttatcttat caccggtggg gctggcttca ttggttctac
      361 cttagccaat catttaggag aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg
      421 aaaagtagaa aatcttgata tgacgaaaaa tatcactttt attgagggag atgtcgctga
      481 gaaatccttg atggaagaga tcatgaaagc taataaattt gattatatct ttcatttagc
      541 agcagttgct agtgtggcag attcagttgc ccgaccagta gaaacacatc gtgtgaattt
      601 tgaaagtgta ttaatgattt tagaattgat ccgcaagtat caacctgatt taaaacggat
      661 cgtgtttagt tcttcagcgg ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga
      721 gtccgtgatt cgcccattga cgccttatgc ggttgataaa ttcgctgcag aacaatatgt
      781 gctagattat tgtcatttat atgatgtacc cggtagcgca gttcgttttt tcaatgtcta
      841 tggtccaaat caaaatccga attctccata cagtggtgtg atttctattc tcgtagatcg
      901 ctataagaaa caaatcgtag gagaagcaac ttcatttaca ttatttggtg atggaagcca
      961 gtctagagac tttgtttata tagatgatgt aatccaagca ttacttttag ttgcgaatga
     1021 ggaagctgca ctggggaaac aattcaatgt tggaacgggt aaagcaacca cacttttgga
     1081 attgattcat gcaatcaatg aaattctcgg tgtagaattg acacttgaat acaaagaaga
     1141 gcgttcgggt gatatccatg actctctagc agatattact aagatccagt caattggcta
     1201 tcaaccgaaa tatggtattt taagtggaat gactaattat ttgaaaactg aaatcaatta
     1261 aaacatacta agataagatt agagggtaaa ttcaggctct ttgtcaataa ggactgatga
     1321 gttttgtaaa atcaaatctg ggatagaa
//
LOCUS       DAFFSV010000256         1324 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.260, whole genome shotgun sequence.
ACCESSION   DAFFSV010000256 DAFFSV010000000
VERSION     DAFFSV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.260"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(293..907)
                     /locus_tag="LR110_002968"
     CDS             complement(293..907)
                     /locus_tag="LR110_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL4823876.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          431 a          262 c          220 g          411 t
ORIGIN      
        1 agagacaaga aagaatccat atggattctt tcttgtctct ttttccacta ctttttattt
       61 ttctgacact acatattctt ataactcatt tctcataact gtgaaacaac ttataaaaat
      121 aacaaacttt aaacaaaaca agcttaaaat caaagatgat ccatcttaaa ataactaaaa
      181 caaaaaaagt agtcgttgtt aatcctcatt aataatcgtt ttcttttttt caaacgctcc
      241 atttttatgt ggaacattat ttttttaagg aactttgtca acatgtggat aactattttc
      301 cacttgtgtc ctggactagc tgttttgctg taaaatacaa aacttgctgc agcaaaaaga
      361 tcgttgcaag agaggctgtt ccgcagatcg ctggatctag atttggcaaa gtcaagactg
      421 catcatgtaa attggcgtgg ttgtccaaaa ccaaatcagc aaattcaaat aatcgcaaac
      481 cactgctatg ttgcgatttg atacttcgtg acaaatcaaa tccagtaaca atgattaatg
      541 gatgaccatt ttctttgatc cactgagcta gttcgatgat tgccgggttt cttccttcat
      601 tcgaaagaag aagaaaaatg tcttctgatt tgacagaaag ctgatcaata attgcctgtc
      661 ctgcaccttc tagcgtttca tagacacctc cggctggatt ttcaatcaat atacttggta
      721 caagatccgg ggtttgatcc caaaattcaa atgctacacc acttagtgtg cggctagcat
      781 atatctgcaa tctaccacct tgattaattt gtttcgcgat ccaagtgcca gcttgaagca
      841 tcagcggctc ttctatcctt aatttctctt ccaattggtg ataatagtca aaataacata
      901 aactcattta tctctcccgc ctagctatac ttgacatgaa tagaaacagc tgcctaatct
      961 aaggaagtac ataaaaaaac agtagttaga cagtaacaca tgtggtcttt gctgtttcaa
     1021 acacaaaaaa ggcattgaac gaatacgccc aataccttga catagcaaat aaaacaacgt
     1081 gcagaccatg gaaaatatct acataggggt agaattttca ccctagatta ggtacaataa
     1141 attaggtcga tgcactgcat tgactatggc aaaatgatcg gtcagggtgt atgtgtgtga
     1201 ctggtcattc cctgattctt agcgccaact aagggtcagg gagccttttt ttattcagct
     1261 gtttctatct gaatttattg taagtaatct cttatccaag tgcaagcaag aaaatacaaa
     1321 aaaa
//
LOCUS       DAFFSV010000257         1312 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.261, whole genome shotgun sequence.
ACCESSION   DAFFSV010000257 DAFFSV010000000
VERSION     DAFFSV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.261"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..690
                     /locus_tag="LR110_002969"
     CDS             <1..690
                     /locus_tag="LR110_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL4823877.1"
                     /translation="NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSE
                     KAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
     gene            824..>1312
                     /locus_tag="LR110_002970"
     CDS             824..>1312
                     /locus_tag="LR110_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL4823878.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVE"
BASE COUNT          530 a          158 c          258 g          366 t
ORIGIN      
        1 aatcctgaag gacaaaaagt gactacagac ttaaataaag agccagacgc atctgaggga
       61 attaagaaca aagaagatct accaaaagga actacttata cttggaaaga gaaagtagat
      121 gttagtacag ctggaaacaa aaaagggatt gtagtagtaa catatccaga tggatctaaa
      181 gaagaagtag aggttactat ttctgtagaa gataaaaaag caccgaataa acctcaagtt
      241 gatcctatta cagagggtga ccaaattgtt actggtaaaa ctgaaccaaa tgcagaggtg
      301 acagttacat taccaaacgg gagtcaatac catgggacag ctgataaaaa tggtaatttt
      361 acagttaaag ttcctaaatt agaggcagga acaaaagtga tagtaactgc aactgatgaa
      421 tctggtaata ctagtgaacc tactaatgta gttgtttctt caaacgaaaa agacagtgaa
      481 aaagccgtta gtaaagataa taagactgac aatcaaggta gcaagcaaaa caaaaataga
      541 ggcaaaagca gtcctcaaaa gcagtcctca aaagcttatc ctaagacggg agagattgat
      601 agtaatatct ttactattag tggtggttta atcctgttag gaactttagg gttattaggg
      661 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaaatct tctctgaaaa
      721 gaggaagaat ctttttcttc ctctttttgt tttttatttt gggagatagg ttgttagtta
      781 aaaatgaaga tacttttcat agaagggaaa agaatgtgat taagtgaaaa aaatagtaat
      841 tattggatca aaccattcgg ggctagctgc agctcgattt ctaaacttat gtgattcaaa
      901 ttatgaagtt attctgatag ataaaaaatc agaaattagt tacttagaat gtgggacatc
      961 gttgttcata aaaagagaag ttagcaattt agaagatctt ttttatcaaa atagaagaga
     1021 actaaattct aagataaaaa agttgttttt ggagacagca gttgacaaaa ttgatattca
     1081 aaataaaaag attatttgtg taaaaaaaga tggcacaaga ttttcccaag aatatgacaa
     1141 acttatacta gctacaggtt caaagcaaat tatgttagat gtaccgggga ataatttagc
     1201 taatattttt tcgataaaga attttccaaa tgctaaagag atttttaaag ctttagatga
     1261 taatactatt aaaaaagtag gaattattgg agcaggatat attggagttg aa
//
LOCUS       DAFFSV010000258         1302 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.262, whole genome shotgun sequence.
ACCESSION   DAFFSV010000258 DAFFSV010000000
VERSION     DAFFSV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.262"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(111..437)
                     /locus_tag="LR110_002971"
     CDS             complement(111..437)
                     /locus_tag="LR110_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823879.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(424..1227)
                     /locus_tag="LR110_002972"
     CDS             complement(424..1227)
                     /locus_tag="LR110_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL4823880.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          370 a          229 c          187 g          516 t
ORIGIN      
        1 acgatatcgt aatattacaa tatgaagata agcgacttat cagctttgat actactgatt
       61 tttataaaaa aaatatcacg atattatgat atcgtgatat tgaatgtaaa ttaaatttta
      121 ttttgtttct ttgctagttt atatccttca atcaagtatt cttcaggtat ggattttaat
      181 agcgcatctt ctataacgtc tggtttactt ttgtctgatt cagcaatagt cgtccaatat
      241 ttgagtgctg acattagaat tggatttata cgaatgctat ctgctgttct tacactactt
      301 ccttgatctt ttatgacttt acttatattt ttcttggtta attcgatttc ttctacttct
      361 tttggtttgt agttcttttt atttttattt cctagtagtc tacttactgc agcttgactg
      421 cgattatctt ctgccatcaa aatcaccaac ttcttttaaa tattcttctg taaatgcttt
      481 atagtcatct tcagctgttg agggtttatt aaaattgatt agatctgatc cagcagcttg
      541 cgcttcgtca acggttattg ttctacgaat ataagttttg aaaatttttg caccaatgac
      601 tctagaaatt tcggttgctg cagtcgtcat ttctttagaa atgttataac gcttattata
      661 cttagtaaat aggactcctc gtacttcaag cttgtcgttc gtatattctt ttaccgtttc
      721 aatagtttca cttaacttta ttagtccttt taatgagaaa tacgcagctt caactggaat
      781 gataatttca tcagaaacag taaatgcgtt gactgtaagc attcctaaac ttggtggaca
      841 atcaattata atataatcat agttgtcctt gattggttga agtactcttt taagcctgtg
      901 ttcacttcct acttgagtaa attccctctc tgcagaagaa agggctatgt cggctggcaa
      961 aacatcaatt cctttatagt ccagaatagc atctgtaatg tcagcctccc cttttaatac
     1021 ttcataaatt gtaggttggt ttgttccatc tatatcgaaa cctacattat ctgtagcatt
     1081 tccctgagga tcaatgtcca ctaaaagtac atttttaccg taaacaaatt tcaaacatgc
     1141 tgctaaatta acgctagtcg tagttttaga aactccacct ttttgattgg ctactgacaa
     1201 tattcttgcc attaaaatat cccccattca tcttatatta taatataatt atatcataat
     1261 attgtgatat tgcaatatcg tatatgcatt atattgtaat at
//
LOCUS       DAFFSV010000259         1302 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.263, whole genome shotgun sequence.
ACCESSION   DAFFSV010000259 DAFFSV010000000
VERSION     DAFFSV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.263"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            376..708
                     /locus_tag="LR110_002973"
     CDS             376..708
                     /locus_tag="LR110_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4823881.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            618..821
                     /locus_tag="LR110_002974"
     CDS             618..821
                     /locus_tag="LR110_002974"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL4823882.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            1005..1160
                     /locus_tag="LR110_002975"
     CDS             1005..1160
                     /locus_tag="LR110_002975"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL4823883.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT          466 a          231 c          229 g          376 t
ORIGIN      
        1 attttaatta ttaacgtttt ttaattcgtt tttagcttaa aaataacttt tgtcacagtc
       61 tcactcctaa aattacttta tttacgtacg aagtgggcag gaggtcgaat ccagaactgg
      121 gccatggtac ttcaacgagg acttctcctc tagaattgaa aagcatgcga tttaccttcc
      181 gtaacgacgg tagatttatt caactatgtg tccaatcaaa atttgtctga tttcaaaaat
      241 agtcaagaga attttggttg ttaaaaactc ttatctatca acttccacag tttagttgac
      301 aaaccctgat actaagttgg gctatgatac acttttcttg actatcagtt acgttaccgg
      361 aaggaatgaa ctctattgaa aaaaacatat gatcgtaaat ttaaactaaa gatttcacag
      421 gatatccttg agaaaaaagt aactaccaag aaaattgctg aagaatacaa tatctctcgt
      481 ccaacaattt cccgatgggt ttcagaatat cgtcgatacg ggaaaaatgc ttttgctgga
      541 caaggaaaaa gattacctga taaagcagat ttctacattc ttgaacagga aaataaacga
      601 ctaaaagagg aagacgagtg ttctacgaac acaaagatcg atacggatct ccagggatca
      661 ctcaaaaact ttacgatgaa ggaatcgaaa tcaataaacg tgttgtagct gttcttatgc
      721 gtgaaatgaa tttatgcaca agaggatttc atcgacgcaa atcttctcat ggtagagaaa
      781 aaacaatcaa agaaatggta aaaaaaatgt tgaatcgata atttgaacag aaccaaattg
      841 atgctgtttg ggtgacggat attacttaca ttccatgtaa tgacggtcgc ctctatttgt
      901 ccacctatat tgacttagca actcgtattc tacgttacta tatggtggat tcacacatga
      961 aaaaagagat tgtcgtccgt ccattacaaa tttattaagg agaattgcct acggtaattc
     1021 attccgatag aggtagccag catcggtctt ttatttatca agagctgttg accgaccacc
     1081 acatcacaca ttcaatgagc caaactggga ctcctgttga caatgcagta attgaagcgt
     1141 ttcatcgatc aatcaaatga gaattaatcg actccaacaa atataaaagt aaaatagaga
     1201 tgaaggtgtt aatccaagat tatttagaag actactaccc aaacaaaaga atccatacaa
     1261 aatttatgat gactccttga caatttgaag cgaagaaaat aa
//
LOCUS       DAFFSV010000260         1294 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.264, whole genome shotgun sequence.
ACCESSION   DAFFSV010000260 DAFFSV010000000
VERSION     DAFFSV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.264"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1244
                     /locus_tag="LR110_002976"
     CDS             <1..1244
                     /locus_tag="LR110_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL4823884.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAFFSV010000261         1271 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.265, whole genome shotgun sequence.
ACCESSION   DAFFSV010000261 DAFFSV010000000
VERSION     DAFFSV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.265"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            120..1160
                     /locus_tag="LR110_002977"
     CDS             120..1160
                     /locus_tag="LR110_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL4823885.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
BASE COUNT          519 a          177 c          205 g          370 t
ORIGIN      
        1 tttaacaaag tttggcgact tatcagcagt agaaaaatat atagtggata gatatatttg
       61 tttaattcat tgtaacatag ttttgaacaa aacgctatcc tacagaaaga ggattggcta
      121 tgaatgattt taattattac aaatcaaaag aaatttatcg tgaaaaatat tatcaaatgc
      181 ctaaggtatt ttttactaac gaaaaataca tggatctttc aaatgatgca aaaattgctt
      241 atatgctact taaagatcga tttgattatt cagtcaaaaa taactgggta gattctgatg
      301 ataatatatt cttcatattc acagttgaag aattaatgaa attgttacaa tgccgcgaag
      361 gtaaagtatc taagatcaaa aaagaattag aaaaagcagg tcttctgaaa cagaagagag
      421 gaagagtaaa taaacgtgat ggtaagattg aatctatgcc taatttattg tacttaggca
      481 agccagaagt tacgaatcaa gacgtcttta aaatcatgga agaggaagac aatactgatt
      541 ccaccgtaat tgcgaaaatc gcaaatacgg caaaaccgcg aaaaatcaat gattctaccg
      601 taattgcgaa aatcgcaaat acggcaaaat ccagtcatat caaggactca accgtaattg
      661 cgaaaatcgc agacaatcta ttctattcta attctataga cactaataga cacataatag
      721 acactgaaac tgaccagtta caaaatcaag ttttgctaga taacttcgtc gacatcatgc
      781 aagaggacag tataaataca tttgtaccag aaaatgtatt aaacttgata aaaacatttt
      841 cttcaagtta cagtgaagct caacaaaccg tccaaactat tcataatgca aagaaaaaag
      901 ctgaagaatt agagcaaact attgtcgttt atgaagaact ggaatcttat ggaattgatg
      961 ctgacaaagg attatatatg actttactga aagcttatca aaaacaaaaa actgaaaaag
     1021 taaacaattt gcagaatcta attttcgttt atgtaaaaaa ttggtttgtc gaaaaagcta
     1081 ttgcggcaaa gcttgcaaac gaacaaagag aaacatttaa cgaattacca acagttagta
     1141 cggaaaattg gttagagtag ttgataaaaa gcaattacta taaaaatgat caattttaaa
     1201 ttagaacttt tttatgattt taattttatt acaaatatta caaataaata gttagtattc
     1261 cggagttttt a
//
LOCUS       DAFFSV010000262         1249 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.266, whole genome shotgun sequence.
ACCESSION   DAFFSV010000262 DAFFSV010000000
VERSION     DAFFSV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.266"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>1249
                     /locus_tag="LR110_002978"
     rRNA            <1..>1249
                     /locus_tag="LR110_002978"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          327 a          282 c          384 g          256 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccc
//
LOCUS       DAFFSV010000263         1242 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.267, whole genome shotgun sequence.
ACCESSION   DAFFSV010000263 DAFFSV010000000
VERSION     DAFFSV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.267"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            67..1152
                     /gene="xerS"
                     /locus_tag="LR110_002979"
     CDS             67..1152
                     /gene="xerS"
                     /locus_tag="LR110_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBL4823886.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          495 a          208 c          190 g          349 t
ORIGIN      
        1 tctataattg gtatagtcca attatagaga tactcctctc ccctacttat cgaaaggatg
       61 aataacatga aaaatacaga aatatatcaa aaaatagata caatcatact tcatatgccc
      121 gattatataa aagattttgt tcaagatcgt gaggataaag atcaatcacc tcgaactact
      181 ttagaatatc taaaaaatta taagctcttt tatgaatggc ttctgagtga gtctatcgtt
      241 cccgatactt caatcacctc gataagacag ttaactgcct atgatctaaa cttgtacaaa
      301 tctcatttga aacgtcgcac aaaggaaaat gtaaaaaaag agacaacaaa aactaagctc
      361 aaagataata ataacttagg tctatctact tctactatca atagaaatat cacagcatta
      421 aaggttctat ttaaatatct ttcgaaaagt tccaataatc ctttaggaaa accatactta
      481 gaaaataatc ctatggatca agtagcaact atcacagata aaactacgtt agctgcccgt
      541 gcaaacgcaa tcgagaaaaa attatttcta gacgaagata cacaaaatta tctagattat
      601 attgccaatg aatataaaaa aactttgagc aaacgagctt tgatctatta tcaacgagat
      661 gtagaaagag acctggcgat caatgcattg attttaggat ctggattaag actctccgag
      721 gttgtaaata ttaatttaga ggacctatct ttagaaaaga ataatgtggt agtaactaga
      781 aaaggaaata agagagacgc agtaaatata gcagcatttg caatggaata tttagctaat
      841 tatctagcta ttcggaaaga acgatacaaa gtaacagaaa acgaaaaagc tttatttcta
      901 gcaatttatc aaggagaagc caagcgtata tccggtattg caatcgaacg gatggtagca
      961 aaatattcaa aaggattcag agtccaagtc agtccacata aattacgtca tactttggct
     1021 actagactct atcaacagac aaattcattg gtattaactg cccaacaatt gggacataca
     1081 agtacaaata caacaacttt atatactcat atagacaatg cagctacaat tgatgctttg
     1141 aacgctttgt aaatgtagta aaaaatagta taataatgaa gcctgtctct aaaattagat
     1201 aaaagcccaa ctttaaaggc aagcttcatt atcattttta ta
//
LOCUS       DAFFSV010000264         1241 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.268, whole genome shotgun sequence.
ACCESSION   DAFFSV010000264 DAFFSV010000000
VERSION     DAFFSV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.268"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            110..982
                     /locus_tag="LR110_002980"
     CDS             110..982
                     /locus_tag="LR110_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL4823887.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          369 a          228 c          287 g          357 t
ORIGIN      
        1 aaaaatagta gttatttact tgtcatgtta ctaaataaaa aaataattta aatatgatat
       61 atagtcattt atttagtgat ttcattctaa aataaaggga gaaatgtcta tgctatacgg
      121 tgcaattgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag aaatgacgat
      181 caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatgcctt tagtcattga
      241 cttcttcaaa caataccaag cagatttagc agggattggg attggctcat ttgggcctat
      301 cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga aattggcatg
      361 gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga tttcttggac
      421 aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca aaggattgag
      481 cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac aagaagggcg
      541 ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa
      601 agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga tggccgctgg
      661 acccgccatc gaaaaccgct tagggaaaaa aggacaagag gttgcagaag atgatcctta
      721 ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct tgatgttttc
      781 tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc tgcaaaacgt
      841 ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt taagcaaata
      901 tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg cattggctcg
      961 agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg ttggtagaga
     1021 aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact ggtttttgtt
     1081 ttacttggaa gatcaagcct tgaatgagat aaattcttgc tcgaaagtta tggaaatttc
     1141 gatagccgta ggccacttgt attgtgcaat agttttttac aaaaaaaagg gggtgcggat
     1201 atttttttgg acctttttaa caaatatatc tttgcttctt c
//
LOCUS       DAFFSV010000265         1236 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.269, whole genome shotgun sequence.
ACCESSION   DAFFSV010000265 DAFFSV010000000
VERSION     DAFFSV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.269"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1007
                     /locus_tag="LR110_002981"
     CDS             <1..1007
                     /locus_tag="LR110_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL4823888.1"
                     /translation="FKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFYM
                     QQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVM
                     GTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFS
                     CVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKF
                     GETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIE
                     RSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW
                     "
     gene            1019..>1236
                     /locus_tag="LR110_002982"
     CDS             1019..>1236
                     /locus_tag="LR110_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HBL4823889.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEML"
BASE COUNT          487 a          161 c          234 g          354 t
ORIGIN      
        1 tgtttaaagg aaaaacaata ttagtaactg gcggaactgg ttccattgga agtgaaattg
       61 ttcgtcagct tttaaaatat gaaccaaaag ctatcagaat ttatagtaga ggtgaagaaa
      121 aacaatttta tatgcaacaa gagctctcaa atcacactaa tttgagattt ttaattggtg
      181 atatcagaga ctctcaaagg attgattatg catgtcgtga tgtggatatt atttttcatg
      241 ctgccgcaat gaaacacgtt ccagcaagtg aatataatcc tatggaagct gtaaaaacaa
      301 atgtgatggg aactcaaaat gtaatagatg cagcgatcaa aaataatgta aaacattttg
      361 taggtatcag tacagataaa gtagtgagtc ctactaacac catgggtgcg acaaagttat
      421 tgagtgaaaa attaataata gctgccaatc agtataaagg aaaaacagaa acaatatttt
      481 cttgtgtaag gtttggaaat gtcatggggt caagtggatc tgttattcct ctttttagta
      541 aacaaataaa aaacaataaa gatattactt tgacagatgt tgatatgaca cgatttatga
      601 tgtcaatacc ggatgctgca agattagttc tgtctgctgc tatgaattca aaatttggag
      661 aaacatatgt cttaaaaatg ccggcaatca atattttaac tttagctcaa tcattaataa
      721 aaatatataa tgatacacat gatgaaaaat atagaggtga aataaaagtt gtaggtatca
      781 ggccaggaga aaaattatat gaagagctaa tgactacaga cgaaattgaa cgctcatatg
      841 aaaatgataa aatgtatatc attccaccac actatcatgc tactgacgaa ctgtacaaag
      901 attatgaaaa aattaaaatc actgcatact cttcagatgc aacagaatta atgagtggac
      961 aggaatttat tagaatcgca aaagagaata atgtcgtgac ttggtaagga gttttatgat
     1021 gaaatcagaa gtatatgtaa ttgcagaaat tggtgtaaac cataatggaa gtctagattt
     1081 agctttagaa agtattgata aagctaaaga atgtggagct aacgcggtta aatttcaaac
     1141 atttaaaacg aataagctag tttccaaaaa ggctccaatg gcaaaatatc agaaggataa
     1201 tcttaaagag gacagttctc aatttgaaat gctaaa
//
LOCUS       DAFFSV010000266         1234 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.270, whole genome shotgun sequence.
ACCESSION   DAFFSV010000266 DAFFSV010000000
VERSION     DAFFSV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.270"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..750
                     /locus_tag="LR110_002983"
     CDS             <1..750
                     /locus_tag="LR110_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBL4823890.1"
                     /translation="IFFYYSTLFDSRFKTLQQNQPEDMELFPPEEKGFSYLMLFDDYN
                     KIDLTLLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCN
                     EFWNVTPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFI
                     ERYISEDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNIT
                     KYTRDMYKKYTGKTGCLDSTYAADIEERREQ"
     gene            747..995
                     /locus_tag="LR110_002984"
     CDS             747..995
                     /locus_tag="LR110_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HBL4823891.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(998..>1234)
                     /locus_tag="LR110_002985"
     CDS             complement(998..>1234)
                     /locus_tag="LR110_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBL4823892.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT          412 a          192 c          258 g          372 t
ORIGIN      
        1 atctttttct attattctac cttatttgat agtagattta aaactttgca acagaaccag
       61 ccggaggata tggaattatt cccacctgaa gaaaagggat tttcctatct tatgctattt
      121 gatgattaca ataaaattga tcttacctta ttgcccttgg aagagttaga taattaccta
      181 aagggcgata aattaataaa ggttctaatt gataaagatt gtagaattaa aagggacata
      241 gttccgactg atatagatta tcatgtaaga aagccaagcg caagggagta tgatgattgc
      301 tgcaatgaat tttggaatgt aacaccttat gttattaaag gattgtgccg taaggaaatt
      361 ttatttgcta ttgatcattt taatcagatt gttcgccatg agctgctgag aatgatatca
      421 tggaaggtcg gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta taagtttatt
      481 gaaaggtata tatccgagga tttgtgggag aaacttttgt ccacctaccg gatggattcc
      541 tatgaaaaca tatgggaagc attatttcta tgccatcaat tgttcagggc ggtatccggt
      601 gaggtggcgg aaaggcttca ttatgcctat ccggagtatg ataggaatat aacaaaatat
      661 accagggaca tgtataaaaa atacactggt aaaaccggct gcctggatag cacatatgcc
      721 gctgatatag aagagaggcg ggaacagtga ttacagaaat gaaagcaggg cacctgaaag
      781 atatcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa
      841 acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag
      901 acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata
      961 agataatata tcttttaacc cgtggtgcta gttaatttta atatcctaaa gaatatctca
     1021 aagttcctcc aaaacgtcgt tgtgctcgac ttaatagaac actgtaagcc agtaatatgc
     1081 tttcaaatct actctctagc cctttaacag aacgtgagtt gaaattttga caacctaatt
     1141 tttctaagga agaaaaaaca gtctcaactg tttttctttg ttttccaagt aagctgtggt
     1201 ctagtttttc tgatttatcc atgtttttac gtgg
//
LOCUS       DAFFSV010000267         1228 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.271, whole genome shotgun sequence.
ACCESSION   DAFFSV010000267 DAFFSV010000000
VERSION     DAFFSV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.271"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..173
                     /locus_tag="LR110_002986"
     CDS             <1..173
                     /locus_tag="LR110_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL4823893.1"
                     /translation="NKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQ
                     QVTDTLVSMFTE"
     gene            596..1228
                     /locus_tag="LR110_002987"
                     /pseudo
     CDS             596..1228
                     /locus_tag="LR110_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          431 a          220 c          217 g          360 t
ORIGIN      
        1 tcaataagca aattaaaaga tacagccgta gaaaagagca gtttcaaaat gaagaatcac
       61 tagaacgctt tctagtcagc atttttgata catacaatca aaaatttcta aacagaagcc
      121 ataaaggttt tcaacaggta accgatacat tagtttcaat gtttactgag taactaatta
      181 ttttgcagga ggacaattta tttacacaaa attattgacg ctccctgttt caaagttagt
      241 agagaaaatg tagttatata tttaaatttt taccagaaag aaatgaacac cttcaattat
      301 ctaaatctcg aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta
      361 ttcatggtct tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa
      421 gatgtgcaag agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat
      481 attacagaca aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat
      541 ttatgggact gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat
      601 tcgtacattg aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa
      661 agtattgatg gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat
      721 ttgtgcggat ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg
      781 attaacacac tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga
      841 tatgaacgcc gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat
      901 tgattgtttt catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga
      961 aatgaaaaga ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg
     1021 gcgctttctt ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag
     1081 ttttcgtgca cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc
     1141 attttcaacg cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag
     1201 agacaaggaa tcagacttat tttttacc
//
LOCUS       DAFFSV010000268         1195 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.272, whole genome shotgun sequence.
ACCESSION   DAFFSV010000268 DAFFSV010000000
VERSION     DAFFSV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.272"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1076
                     /locus_tag="LR110_002988"
     CDS             <1..1076
                     /locus_tag="LR110_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL4823894.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAFFSV010000269         1183 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.273, whole genome shotgun sequence.
ACCESSION   DAFFSV010000269 DAFFSV010000000
VERSION     DAFFSV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.273"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..197
                     /locus_tag="LR110_002989"
     CDS             <1..197
                     /locus_tag="LR110_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823895.1"
                     /translation="VFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICK
                     KVCNGEENYWIGEEKLNEIY"
     gene            184..636
                     /locus_tag="LR110_002990"
     CDS             184..636
                     /locus_tag="LR110_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823896.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            629..1027
                     /locus_tag="LR110_002991"
     CDS             629..1027
                     /locus_tag="LR110_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823897.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
BASE COUNT          455 a          145 c          211 g          372 t
ORIGIN      
        1 ttgttttttt cgtatacaat tctatagaag atgctactgc gactactgca ttaacattcg
       61 gttttacgaa aaatggaaca atcaattcgg ataaaacccg tcaagctgct acagaaagtg
      121 atgatatatg taagaaagta tgcaatgggg aagagaatta ctggataggg gaggaaaagt
      181 tgaatgaaat ctattaatga tttattaaaa aaacatcctg atttatataa aaaattaaat
      241 tctgaacaaa gaaacctgtc caaacaatta cttaaaataa gattaaaaaa acaaaaaaac
      301 gtgacagaat tctcaaaaat gttaggaata agtgaaaaaa aatatcttga atttgagttc
      361 ggagatatgc atattcctgt tgcagagtac aataaacttt tgaaacaagt tgaaaatttg
      421 tcctataaag attcaatgaa tttcactatt aaggttgctg gcggccctat taaatatgac
      481 cgtattgaaa ataaacctta tattcggaat aaaagttact cagtatttaa aagtagaaag
      541 agaaaagaag tatcgtcaaa tatggtctat gctagtgtta attaccaaga tacgagccgc
      601 tatattgtag aaaaagaggt atatgtacat ggataaagga aatagtgtta cttttgcact
      661 tgaaaatatt agtttgaata aaattcattt tgaattaact gataatttta gttatggaga
      721 tttagagttt aatattgaac ttggatataa agaagacgaa gcagagtatc tagaaaaaaa
      781 acttgtttct ttacgagctc acattgtagg ggggatggaa ggagcaccgg tagatatttt
      841 actcttttca gttattcgtg tttctacaaa tgaacagcca tataaaaaat tagtagaaga
      901 atctatggga gagctagcta aacctatcct gaataaagct tctaatttga tttctaattt
      961 atatagcgaa gtaggagtta tgcctacagt tattgattta tatcagctat atagtcaaca
     1021 aaaataaaat acaagtaata gagttatcta tttaaatttg caagacaaag acctcaacca
     1081 gtcatccttg gttgaggctt ttttgctctg taattaactc gtatagatcc tgcaagatag
     1141 tttgtttctg aataaatgat tgatatgaag tatttagatg act
//
LOCUS       DAFFSV010000270         1144 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.274, whole genome shotgun sequence.
ACCESSION   DAFFSV010000270 DAFFSV010000000
VERSION     DAFFSV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.274"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..820)
                     /locus_tag="LR110_002992"
     CDS             complement(<1..820)
                     /locus_tag="LR110_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBL4823898.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEE"
     gene            complement(843..>1144)
                     /locus_tag="LR110_002993"
     CDS             complement(843..>1144)
                     /locus_tag="LR110_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBL4823899.1"
                     /translation="YDLIINIANVNPGTVQRIVWPASKGTPDIPFYIHEIPTIFVSVQ
                     CPFHLADVPQVKTYINTYDGKQETLEILVDKLMGLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT          360 a          225 c          145 g          414 t
ORIGIN      
        1 attcttctgt ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat
       61 ataaataagc gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct
      121 ttccttcagc ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc
      181 cggtccattc tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat
      241 taatagtaat atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa
      301 tacctgcata attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt
      361 cctgtactgt ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta
      421 cagcaactgt aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg
      481 gtgtaaaccc acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat
      541 gtgttgcaga tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca
      601 cttctttttc ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat
      661 cattataaga agagggaact gaaatcggat aactctcttt ttcatttatt ggttctaaag
      721 caagttcttc ctgtaagtca tcatttgtat ctaatttaaa tctccagaga ccgcttaagt
      781 caattacttg cctcgtctct gtcataatgg gatacaacat ataaaccact cctatctaaa
      841 tattaaataa atgtatcctt taaaccacaa taagcatcta ttggacttac tcccttaaat
      901 tcagaaagac ccattagttt gtccaccaat atttctaaag tttcttgttt tccatcataa
      961 gtattaatat aagtctttac ttgaggtaca tcagccaaat gaaacggaca ctgtacagat
     1021 acaaaaattg ttggaatttc atgaatgtaa aacggtatat ccggagttcc tttacttgct
     1081 ggccagacaa ttcgttgaac tgtgcctgga ttaacattag ctatattaat gatcaagtca
     1141 tatt
//
LOCUS       DAFFSV010000271         1132 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.275, whole genome shotgun sequence.
ACCESSION   DAFFSV010000271 DAFFSV010000000
VERSION     DAFFSV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.275"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            81..341
                     /locus_tag="LR110_002994"
     CDS             81..341
                     /locus_tag="LR110_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823900.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(703..942)
                     /locus_tag="LR110_002995"
     CDS             complement(703..942)
                     /locus_tag="LR110_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823901.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          401 a          164 c          204 g          363 t
ORIGIN      
        1 aagtgttatt gattttcata atcatatttt aatggtatta tcattttaaa taatggaaaa
       61 acattaagga tggatgaaat atgaataacg aaactatcat tttaggaaaa aaatatattt
      121 gccaacctat tggcttgaaa caaaaagtgg ttggagaagt aatcagtaaa ttagaaaatt
      181 gtgttgtgat ctgcgtagac gaatatcatg acgtcgatcg caatgaaata ttagaaaaat
      241 gcggaaaagt agtggcaaaa tatactcacg tttatgagtt ggaaacaaca gaatatctat
      301 ataactatag caacaaatat gaacatactt tagtttcata aagatttaaa taaagtgcct
      361 tcaaaagcga gaagaccaag cttttgaagg cactttattt agttggcagt gattgttcat
      421 gatttttgat ttattcaaaa ggttcgccta gagaatctgt ctctgtaacg aggcctggtg
      481 gagaaactcg ataaaaacct atactgccag aactgtccta ttttcggata gacaaactag
      541 tagttttaaa aagatcatcc gatccaatca tttcaaaaaa tgcaaaactg atatcaggat
      601 catttagctc aggcttgtta tcgtaaagca tttgtaaagg ttgcatttag ttaggagtaa
      661 acgcgaaact atcatcatta tttgctgaag aagatgaaga agctaattca gagtttgttt
      721 gaaataaagg agacgtgctg acagtttctt taccctgttt tttgcagagg tagaaacttt
      781 tatatcggaa gtttctaccg gagaattttt ctcatttgta gaaattgttg aatcgatact
      841 attcgaaatt tgcgtatctg aaggtatgtt ctttgatgca gaagtcccac aagcagtaag
      901 taacaataaa tttgataaac tgataaacag aattcttttc attatgtaca cactccttat
      961 agtatcgttt agcatacacc tatgctcagt attaggcaaa atcaatgcaa aactatcaag
     1021 tcttttatag tatctattag tcttagtaat acatatatgg agagggtgat atcccagttc
     1081 aaaccagata cattggttac catctgtatt aacgattatg cctctaaacg ct
//
LOCUS       DAFFSV010000272         1126 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.276, whole genome shotgun sequence.
ACCESSION   DAFFSV010000272 DAFFSV010000000
VERSION     DAFFSV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.276"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..292
                     /locus_tag="LR110_002996"
     CDS             <1..292
                     /locus_tag="LR110_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBL4823902.1"
                     /translation="MESDLRGIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIK
                     KIALASKACGADIQHIGDSLNGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            311..>1126
                     /locus_tag="LR110_002997"
     CDS             311..>1126
                     /locus_tag="LR110_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL4823903.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFL"
BASE COUNT          363 a          168 c          204 g          391 t
ORIGIN      
        1 catggaaagt gatttgcgag gaatcgttga ttatataaaa gattttaata gaaataaatc
       61 tattgaggaa aaagttctag tactaacggc taatggaaca agccaagact cttctgacca
      121 agaaactatt aaaaaaattg cattagcatc aaaagcatgt ggagctgata ttcaacacat
      181 aggggattct ttaaatggta tttgtcttcc agaaaatatt tttgctttgg gtcaagcaat
      241 tcggggatat cgtcatcaaa ttattatgtt aggaaaatct aattatcgtt aattaaggag
      301 gaaatgaaag atgatcgaca aagttcaaaa aatagctgca aaaattagta atcaaagaca
      361 tctactagct ttaagagaca gcatggctgc agttatgcca ttaatcatta ttggttcctt
      421 ttttatgtta ttagcaaatt tcccgataga aggatttaca aaatggttat ctgaaattgg
      481 agtacaatcc tttttgaaca aagcatcaga ttcaacattt ggtattattg gattagctgt
      541 tacctactct ttgggacaca atctagcaaa gacttatggt gtagactcat tatcagcagg
      601 attattatct ttgagtggat atgttttact tacaccttta ttggatgcag atggttctat
      661 aggatttcca acaaaatact ttggtacctc gggtcttttt gttggtatta ttgtagcact
      721 tgtatctaca gaaattttcc ggtggtttgt acttaaaaat attacaatta aaatgccggc
      781 aagcgttcca ccaaatgtaa gccgagcttt cgttgctatt attcctggct tttttgtcgt
      841 tctactatgg tttattgttg ttgtaatttg ttataaactt ggaattgaaa atgttcatgc
      901 tcttattgca gatacactcg caaaaccact atctttactg acgaaaactt taccaggaat
      961 tatattggtt atacttattc aatgtttctt ttggatgttt ggtattcatg gtgcacaggt
     1021 aacggggcca ataattgaac cgttattatt gcaaaactct gatgtaaata gaattgctta
     1081 tcaagctgga aaagaacttc ctaacattat aacttatgaa ttctta
//
LOCUS       DAFFSV010000273         1117 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.277, whole genome shotgun sequence.
ACCESSION   DAFFSV010000273 DAFFSV010000000
VERSION     DAFFSV010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.277"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(32..517)
                     /locus_tag="LR110_002998"
     CDS             complement(32..517)
                     /locus_tag="LR110_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HBL4823904.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(519..>1117)
                     /locus_tag="LR110_002999"
     CDS             complement(519..>1117)
                     /locus_tag="LR110_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823905.1"
                     /translation="KKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRI
                     VSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGI
                     SIFVFGNKLESGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDY
                     FDDSLKSKFQALEKLDTEGEVDKKVEQIVSLINSIQED"
BASE COUNT          340 a          240 c          117 g          420 t
ORIGIN      
        1 atgtcattag atagtagaca gttccaactc tttatttacc ttttttgtat gctgcactgt
       61 caatccaagt tcctaaattg ttgtatccat catcacttat ataatcatat tggccatcaa
      121 taatttcgtc aaaccacaaa tattctccat ctggtttttg cccaattatt ttaattactg
      181 attgactagt agttgtcatc tggttaaaat cttttatttt acctatatta acggccaaaa
      241 tagcatttcc tcgcttataa ctctcctgga tttcatattt aacatattct cgatcaagag
      301 tttctttacc tatcagcacc acagtcactg atgttccaat taattgatta tctatccagt
      361 tttttatagc aatatctcca ttacgtttaa ctttctcaaa ttcagcctta tctacaaaac
      421 cagcttcctt tgttcctcgc aaagtccagc tgtttctaac taccattgct cttgttacat
      481 ctgcataatg gaaactaaaa aaaacccttc tcaccatatt aatcctcctg aatactattt
      541 attaaactga caatttgttc aactttttta tctacttctc cttcagtatc taatttttct
      601 agcgcttgaa atttactttt taaggaatca tcaaaataat cactgaaatt ctcttgaatc
      661 ttatcaaaca aaaccttact tgcacctttg gtaaatccaa tcggaactac tatattttca
      721 ttttgaacag ataattcaaa ttcttcaatc attccatctg ctgtaactat ttcacctgat
      781 tcaagtttgt ttccaaaaac aaaaatacta atcccagact ctgcaataat gttttctcga
      841 tatcttctcc actcatgagg catatcaagg ggaagtggtc ttaaagaaag tacgtcttgt
      901 atgttatttt ttcttaaatt tcttctctgc tttagaactc ctgaaacaat actattacca
      961 attccaagtc cgtaacctga tactattcta taaccggatt cagaaagcct atatgctaat
     1021 ttctcgacaa attttaatcc tttcgattca gatgtaaatg gagtataatc aacagcactt
     1081 cccgaaataa aaatagtttt tctcaaatac ttttttc
//
LOCUS       DAFFSV010000274         1112 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.278, whole genome shotgun sequence.
ACCESSION   DAFFSV010000274 DAFFSV010000000
VERSION     DAFFSV010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.278"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            40..1080
                     /locus_tag="LR110_003000"
     CDS             40..1080
                     /locus_tag="LR110_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL4823906.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          456 a          167 c          189 g          300 t
ORIGIN      
        1 tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt taaatttttt
       61 aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt tattgtttct
      121 gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt aaaagcaaga
      181 ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta ttttacttat
      241 acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa
      301 aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa caaacagtta
      361 gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga aaatgatatc
      421 tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc agagggtatg
      481 aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc tcaagagggt
      541 atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat cataccagaa
      601 cttaataata atatattaga cactaataga cacaatatag acactgaaaa agaccgccta
      661 caagatcaat tgttgttaga caattttgag acaattatga caaacgacag cattgctacg
      721 tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta cagtgaagct
      781 caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga aagtggtatt
      841 tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga attatacacg
      901 acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg
      961 atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt
     1021 gaaaaacgtt tgaattatga aagctcccca agcactatta cgaaagactg gttagagtga
     1081 aactttactg acaaagtgat tgttgcttca tg
//
LOCUS       DAFFSV010000275         1106 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.279, whole genome shotgun sequence.
ACCESSION   DAFFSV010000275 DAFFSV010000000
VERSION     DAFFSV010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.279"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            1..999
                     /locus_tag="LR110_003001"
                     /pseudo
     CDS             1..999
                     /locus_tag="LR110_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          391 a          199 c          222 g          294 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg agacaccggt tggttaccgt aaccagagat tggcgcagcg aatccttgat
      961 aatgagctcg gatgtgctaa cgctagcgag gcagtctaac tttaactgtt agctcctgcc
     1021 gaagatcgtc acatccgagg aggctcattg tcaaggacaa tcggaatagc atacggaaag
     1081 aagttctgca ccttataaaa tttgtc
//
LOCUS       DAFFSV010000276         1083 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.280, whole genome shotgun sequence.
ACCESSION   DAFFSV010000276 DAFFSV010000000
VERSION     DAFFSV010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.280"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(79..152)
                     /locus_tag="LR110_003002"
     tRNA            complement(79..152)
                     /locus_tag="LR110_003002"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Ile,seq:cat)
     gene            complement(707..1021)
                     /locus_tag="LR110_003003"
     CDS             complement(707..1021)
                     /locus_tag="LR110_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823907.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          409 a          166 c          170 g          338 t
ORIGIN      
        1 ggcaatgaag aaaagcttta gcttgtataa tactcttcaa aaaattctaa cacagaacga
       61 ttcaattggc tacattaatg taccctgtag gactcgaacc tacgaccgga cggttatgag
      121 ccgtctgctc tgaccaactg agctaagggt agtggttgtt gccacataaa gccataaaca
      181 atcaaccagt agaatgcgtg gcaacaaacc tgttatcgca tatcttggag tgtgactatt
      241 tatgagtgat agtgaagata tgcgtctcca tatatgtatg gttgagacag ataaacatgt
      301 atgaaaaacg ttgttaaatc aacgtttttt tattttggtc aaggagagaa aagtagagaa
      361 atacaaaatt ttttgcattg attttgcatt caaaaaaaca ttcaaacatt tagaaatccc
      421 caatacgtat tcaggaaaat ctattttaag gtttacaaaa agaacttttg ttcgtacact
      481 ctttttgagg tgaactttta tgctgatgga aggaaaaaca caattatggt ttaagcatgt
      541 tccttcaaat aagtttttta attataaact ctagagaaat taattcatcc taacataaaa
      601 taattgttca ctcaaaagtt tttctagaac aattaaaaca gcaggcttca cattcgctat
      661 acatttgtaa gaagtaataa ttcctgtact aaaaggcata attgctttaa ttattttttg
      721 acttctcttc aaacacaaga agtagttgtg taataattgc agaaataagg attccaataa
      781 tagatataaa atttgacatg gaaacgtcta taacattaaa agaataaagt gttgaagaaa
      841 gtaaactgat tgaaataacc cctccgtaaa aaatagtctg tgaatatacc cacttttttt
      901 cagaagacgt acttcgtttg gttctaaaac ctatccctgc atttctactc ttatcaaaaa
      961 accaaatatt tagaagtaat aatattgcta ttattaagat aaaattactg taaaaatcca
     1021 aaagctatcc gagagtataa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
     1081 gtc
//
LOCUS       DAFFSV010000277         1074 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.282, whole genome shotgun sequence.
ACCESSION   DAFFSV010000277 DAFFSV010000000
VERSION     DAFFSV010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.282"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            6..398
                     /locus_tag="LR110_003004"
     CDS             6..398
                     /locus_tag="LR110_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4823908.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            399..>1074
                     /gene="aac(6')-Ie"
                     /locus_tag="LR110_003005"
     CDS             399..>1074
                     /gene="aac(6')-Ie"
                     /locus_tag="LR110_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HBL4823909.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIF"
BASE COUNT          430 a           83 c          192 g          369 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat aaagatgaaa
      361 tagtatatgt atatgattat gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa
      421 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
      481 aaagagtatt agaattttat ggtggtagag ataaaaaata tacattagaa tcattaaaaa
      541 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
      601 ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt
      661 atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt
      721 ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa
      781 gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat
      841 accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta cacgagggca
      901 aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg
      961 caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa
     1021 tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt ttta
//
LOCUS       DAFFSV010000278         1057 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.283, whole genome shotgun sequence.
ACCESSION   DAFFSV010000278 DAFFSV010000000
VERSION     DAFFSV010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.283"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..869
                     /locus_tag="LR110_003006"
     CDS             <1..869
                     /locus_tag="LR110_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823910.1"
                     /translation="KKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEK
                     SVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIR
                     RELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKF
                     FLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGK
                     PEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQI
                     DDFPEEIIDRIE"
     gene            complement(923..>1057)
                     /locus_tag="LR110_003007"
     CDS             complement(923..>1057)
                     /locus_tag="LR110_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL4823911.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          367 a          158 c          186 g          346 t
ORIGIN      
        1 aaaagaaatt attccacttt ttagcaatca acctgttggc tgaacaagct gattctttag
       61 atattgctag caatcagtcg ctattttttt cggatatagg attttaccac ttaaaaggag
      121 tattaggaga aaaatccgtt atttttatca ataagacact ctcttggctt tatcttcatg
      181 ataaattgga tcaaaaatac ttgagaaaaa atgaagatga aacaatcgaa tggttgaatg
      241 aacgattcat tgaaagtttt gaaaatcagt ttaatctgtt gcgagaagag acgagccaat
      301 tgatccgaag agagttaacc aaaatacatt ttaaactttt gcattacact attaataaag
      361 ctgatgaatt tccacttgat gtttcttttt ttagagaaaa ctatccagca ttttattgct
      421 atttgataga gtatataaat gatctaacgt caaaacgcaa agagttgctt caagataaat
      481 tttttctttt tttcagttat ttattattat tgatcaatca catacccatt caactgattt
      541 ctgaaccagt aaagatcatc atcgattttt cttatggtgc tgcctataat caatttatca
      601 agaaaaattt aagcttgtac attaatctga acacggaagt gatagaggca ggaaagcctg
      661 agttcccgga tgttataatc acgaatagga ataatctata tgaagaaagt gcatctcaag
      721 tagtggtttg gctcgatccg ccaagagcag tagactgggg gaatcttaca aagattttgt
      781 tgactatcca agaagagaaa tacaaagaca gcaaaattga caagcaaata gacgattttc
      841 ctgaagaaat catcgacagg atcgaatagt gaacttcttt tagaaagtcc gactgctttt
      901 tctaaaaata ttatttgtca aattaagcga gacaaaactt tccatttacg taactcaaaa
      961 aaacttctaa tggtgtttgg taatttagtg attttctagg gatatggttt cttttggacg
     1021 caacggatga gatgaatcct tgattgactt ggttgaa
//
LOCUS       DAFFSV010000279         1002 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.284, whole genome shotgun sequence.
ACCESSION   DAFFSV010000279 DAFFSV010000000
VERSION     DAFFSV010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.284"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..445
                     /locus_tag="LR110_003008"
     CDS             <1..445
                     /locus_tag="LR110_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823912.1"
                     /translation="VKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVHEDKVK
                     DQVKDEDYEQHAINYKMKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLPYQVAD
                     DQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            467..697
                     /locus_tag="LR110_003009"
     CDS             467..697
                     /locus_tag="LR110_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823913.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYLAAKKYFTADNK"
BASE COUNT          428 a          147 c          164 g          263 t
ORIGIN      
        1 agtgaagcta tcgcatgtac cagaagaata tattgctatg cgattaacca ttattcctga
       61 aaaaattgat ccaaaggtgg atatgcaaga accacaagat ctgatttatt acgttcacga
      121 agataaggtt aaagatcaag tgaaagatga agattatgaa caacatgcga tcaattacaa
      181 aatgaaagga tacaagaaag aacaaaaagc agctcaaaaa caaatggaat ctttccaagc
      241 aaccattgat ttgaatgaag aattagtgaa gaagctaaaa gatcaattgc cttatcaagt
      301 cgcagatgat caggaaaata ctgaaaacaa gattaatagc taccagcaag aaattgaaac
      361 atcgaaaacg caaatgaaag atcaagaaga gatcattcaa aaacttcaag aaaaaattga
      421 tcgtatgaca aaagaaaaga tttagaagga caggtggagt gaaaaaatga agcgaacaaa
      481 cttggctgac aaagatactt tcgctgagca gaaagtattg attaaagcct atctcaaagt
      541 tgtaaaagct aaagaaatat gtcgagaagt gaatttatct aatacaacaa tatctgagtt
      601 gaagaatggt cgaaagaatt tagatacttt aaaattcaga tcatttgaag ctttgtattt
      661 agctgctaaa aaatatttta cagctgataa caaataaaaa acaggagtat tttatgtgaa
      721 aaaagtatat aaaaaatgct tatgaatcag ctatatcacc ttaaccattt gttaatgaga
      781 tgggagaaaa cattaaccac gattctaacg caaacatcaa gcacataaaa catttaacac
      841 tcctcctttc tggctgaaga tttcaaccaa acaggaagac agcgtatcaa ttatagcata
      901 cactttttct taaattcatt tttcatatga caaaatattt ccttctaatc gacaaagtta
      961 gaaggatttt ttattgtcta aatacacaaa taaccttaat tt
//
LOCUS       DAFFSV010000280         1000 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.285, whole genome shotgun sequence.
ACCESSION   DAFFSV010000280 DAFFSV010000000
VERSION     DAFFSV010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.285"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>1000
                     /locus_tag="LR110_003010"
     CDS             <1..>1000
                     /locus_tag="LR110_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL4823914.1"
                     /translation="NNDAKKFLEENLKKIAVRNFKVSENNFKNLSFTISDSEISELTG
                     DIFNYEIKRYSISGIVITIQNVGEKLKISFEPTDSFQYLSKDIEIEDIYKILAQELSK
                     NVISLPKVFYFPAERTGINVFKNELNESRLKTYDTLLNTVQFTNLTNQKKKAALKYDL
                     LKKNIELLMETTDNSSYPKPISDYINYLNNMKNRYEQKNVNNIASYIRDEIIMGKYEI
                     DEKDNSVSFRQKFGKNRYRKERIPFHVVSSSIKSLYGLDYYLENSSKEGDYLIIDEPE
                     LSLHPENQVKLAKVITDIVESKIKLITSTHSDLFVRELTNIVIENKLNSKNNKKNKL"
BASE COUNT          415 a          110 c          154 g          321 t
ORIGIN      
        1 aataatgatg caaaaaaatt tttagaagaa aatttaaaaa aaattgcagt tcgtaacttt
       61 aaagtaagtg aaaataactt caaaaatctt tcttttacta tttcagattc agaaattagt
      121 gagctcactg gtgatatctt taattatgag atcaaaagat acagtataag tggcattgtt
      181 ataacaattc aaaatgtagg agagaaatta aaaattagct ttgagccaac tgattcattt
      241 cagtatctta gcaaagatat tgaaattgaa gatatttata aaattttagc tcaagagcta
      301 tctaaaaatg ttattagttt gccaaaggtt ttttattttc cagccgaaag aactggtata
      361 aatgtcttta agaacgaact aaatgaaagt cgcttaaaaa catatgatac cttgctgaat
      421 actgtgcaat ttactaattt aactaaccaa aaaaagaaag ctgccctcaa gtacgatctg
      481 ttaaaaaaga atattgagct gttaatggag acaacggata atagttctta tccaaagcca
      541 atatcggatt atattaatta tctgaataac atgaaaaata gatatgaaca gaaaaatgtc
      601 aataatatag catcatatat tagagatgag attattatgg gtaaatatga aattgacgaa
      661 aaagataatt ctgtaagttt tagacaaaag tttggtaaaa atagatatag aaaagaacga
      721 attccttttc acgttgtgtc ttcttcgatt aaatctctat atggattaga ttattactta
      781 gaaaattcaa gtaaagaagg agactatctt attattgatg agccagaatt gagtttacat
      841 ccagaaaatc aggttaagtt agcaaaagta attacagata tagttgagag taaaattaag
      901 ctaattactt ctacccatag tgatttattt gtacgtgagc ttacaaatat tgttattgaa
      961 aataaattaa atagtaagaa taataaaaaa aataaattgt
//
LOCUS       DAFFSV010000281          999 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.286, whole genome shotgun sequence.
ACCESSION   DAFFSV010000281 DAFFSV010000000
VERSION     DAFFSV010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.286"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(62..>999)
                     /locus_tag="LR110_003011"
     CDS             complement(62..>999)
                     /locus_tag="LR110_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBL4823915.1"
                     /translation="PFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTSH
                     YPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKTV
                     IKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLK
                     STPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTEY
                     GADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILRV
                     QGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          308 a          210 c          126 g          355 t
ORIGIN      
        1 aaacaaaaaa aaacgattag gctaggctaa tcgttttttg aacattctta aaaaatccaa
       61 attatttttt ataactgaaa ttgggtattg atgaccatcg tttctgtaga tagtaagcaa
      121 tcattttagg ttttctatct ctagtaaaga ttcctttctt atttccttgc actcttaaaa
      181 tcccttgact tgttgcaaaa tctgcaaaat tccacacttg ttcaccaaca aaattttcac
      241 atgtatctat tacttcgtga tttgcttcgt aataagccac ttgatactct tctgtaaaca
      301 ttgttggtac tgtatcatga aatccagcta ctgtatctgc tccatattct gtaaacatta
      361 ttggcgtatc aggacatctt tccatccatc catctagctc ttttttcaac agatattttg
      421 catgtgccag atcccctccc gaaacatacc aaccataata acgatttaaa cataaaacat
      481 cgacaatatc tcctacctta caaacatctg gcgttgattt tagataagta acgattgtta
      541 caggtctttt ctgcggatca agttctttag ttaattcaac taaaggttca aaatattctt
      601 tagcaccttc ggaatccgaa tctggttcat ttgcaataga ccacattaca acacttgggt
      661 ggttcttgtc gcgttctatc aattccttga tcaccgtttt atgagaattt tctgttcgta
      721 tctctttcca cgtattatgt ttttccacat tatcatctaa taaagtggcc atgaaattta
      781 aatgtaaccc tactgcaggt acttcatcaa taactacaat accttctcga tctgctaatc
      841 gcataatttc ttctgagtaa ggataatgtg atgttctaaa agaattagca cctatccatt
      901 tcattaaatt aaaatcttta atatttacag cctcattcaa accacgtcca ttaacatacg
      961 aatcctcatg cttaccaaat cctttaaaat aaaaaggtt
//
LOCUS       DAFFSV010000282          994 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.288, whole genome shotgun sequence.
ACCESSION   DAFFSV010000282 DAFFSV010000000
VERSION     DAFFSV010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.288"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..>994)
                     /locus_tag="LR110_003012"
     rRNA            complement(<1..>994)
                     /locus_tag="LR110_003012"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAFFSV010000283          985 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.289, whole genome shotgun sequence.
ACCESSION   DAFFSV010000283 DAFFSV010000000
VERSION     DAFFSV010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.289"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          385 a          135 c          151 g          314 t
ORIGIN      
        1 ataatttaaa ataaatggta tacatgaata ggaaaggaaa cagaaggagg caatcttaat
       61 tttttaaatt gtctaaatta tatagaaaga acaatctttc tatataatta gacataagag
      121 agactacggt tgatttgata ttaacaagcg tatatgaatg tatatacaaa taaaaaaggg
      181 gagggagaag ttaaagaaaa ttcagagaag tggggcactc agtagtatta acaatttaaa
      241 aggaatttct taaagaaaat gggttgcatc ctggagatac aattttttta gataaagaaa
      301 aaattcaagc aacaattcaa aaaaatctta actgctgaga ttgatgtagc aatatctcaa
      361 tcatcaacaa agtacgatcc tgcctttcaa aagcttattg ataaatgttt attcattata
      421 aaaataaaag aaaaatttat gtcagctttt ttcatcaaag tggtaatgat aattcgtcta
      481 ttaattgtat tttttaggaa atatggtata attgttttaa cgaaaaggta attcatgata
      541 caccaaaaac cactagctgg cgggctagtg gttttctttt acgttctatt cagattaacg
      601 ggtaggctta ctgtcttttt tggcaataat tttgcaatca agccatttac agatgtaatg
      661 cgcggatact tctgctaaga ttgcaaaaaa catatcgaaa aggtaattca taatacaacc
      721 tcctcccctt gccataatag gccggggtag cagacagcat aaatattata ccataactga
      781 aaatttcatt ttagtacatt tcaatatatt gatgaaccag cacaataaaa catgccatga
      841 ctaacagaaa aaaacttatt aaaccagata taacttgtaa acatccaact atatagaaaa
      901 cgtaaatttt ctatatagtt ggattaagat aacgactatt tattttatca tatttttact
      961 ataccctata cttttacttg gtata
//
LOCUS       DAFFSV010000284          979 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.290, whole genome shotgun sequence.
ACCESSION   DAFFSV010000284 DAFFSV010000000
VERSION     DAFFSV010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.290"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            104..>979
                     /gene="bsh"
                     /locus_tag="LR110_003013"
     CDS             104..>979
                     /gene="bsh"
                     /locus_tag="LR110_003013"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL4823916.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDS"
BASE COUNT          332 a          128 c          184 g          335 t
ORIGIN      
        1 aaataaaaac gcttctttgt gtattctgtg cttttctttt ggaatgagga aaactttgga
       61 gtaatatgaa cgtggacaga aaaatattta ggaggaataa actatgtgta cgtctattac
      121 ttatgtaaca agtgatcatt attttggaag gaattttgat tatgaaatat cttacaatga
      181 agtagtcact gttactccaa gaaattataa gttgaatttt cgaaaggtaa atgatttgga
      241 tactcattat gcaatgattg gtattgccgc tggtatagct gactaccctc tttattacga
      301 tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat ttttctgggt atgctgatta
      361 taaagaaata caagaaggga aagacaatgt atctcctttt gaatttattc cttggatttt
      421 aggacaatgc tcaacagtag gagaagctaa aaaattgtta aaaaatatca atttagcaaa
      481 tataaattat agtgatgaac ttcctttatc ccctttacat tggctattag ctgataaaga
      541 aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat atatatgata accctgtggg
      601 cgttcttacc aataatcctt catttgacta tcaattattt aatttaaaca attatcgtgt
      661 cttatcgagt gaaactccta aaaataattt ttcaaatcaa ataagtttga atgcctatag
      721 ccgcggtatg ggagggatag gcttgcctgg agatttatcc tcagtatctc gttttgttaa
      781 agcgactttt acgaagctga attctgtatc tggagattca gagtcagaaa gtattagtca
      841 atttttccat atcttaggtt cagtagaaca acaaaaaggt ttgtgtgatg ttggtgatgg
      901 aaaatatgaa tatacaattt attcttcttg ttgcaatgtt gacaaaggaa tctattatta
      961 tcgaacatat gaagacagt
//
LOCUS       DAFFSV010000285          975 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.291, whole genome shotgun sequence.
ACCESSION   DAFFSV010000285 DAFFSV010000000
VERSION     DAFFSV010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.291"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            4..>975
                     /locus_tag="LR110_003014"
     CDS             4..>975
                     /locus_tag="LR110_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823917.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQ"
BASE COUNT          413 a          150 c          219 g          193 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
      661 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agagttgatc
      781 gatcaactta aaacgttgga taaccaatac gctgcagaat caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaa
//
LOCUS       DAFFSV010000286          973 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.292, whole genome shotgun sequence.
ACCESSION   DAFFSV010000286 DAFFSV010000000
VERSION     DAFFSV010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.292"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..908
                     /gene="yidC"
                     /locus_tag="LR110_003015"
     CDS             <1..908
                     /gene="yidC"
                     /locus_tag="LR110_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL4823918.1"
                     /translation="ILLFLSGCVRMDSNGNPDTSGIVYRVLVHPMGQAITYLVENFNW
                     SYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVEAAQQKLKAATTREEQ
                     MAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARYTEGIRESSFYGIDLG
                     SPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAMIVFMSISAPAGVTLY
                     WVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPRKDVTESAKPADNKQA
                     TKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT          324 a          212 c          202 g          235 t
ORIGIN      
        1 gtatcctgct ctttttatct ggctgtgtgc ggatggattc aaacggaaac cctgacactt
       61 caggaatcgt ttatcgagta ctcgtccatc ctatgggcca agcgattaca tatctagttg
      121 agaatttcaa ttggtcttac ggctgggcag ttattgcaat gactgtgatc gttcgtatca
      181 tcatcttgcc actaggtatc agtcaatcga aaaaaacaat gatccaatct gaaaaaatgc
      241 aagcgttaaa accacaagtt gaagcagcac aacaaaagct aaaagctgca acaacacgtg
      301 aagaacaaat ggcagcacaa gcagaaatgc agcaagtata tcgcgaaaat ggattgagta
      361 tgaccggcgg aatcggctgt ctgcctctat tgatccaaat gccgatcttc tctgccttgt
      421 attttacggc tcgctacaca gaaggtatcc gtgaatcatc tttttacggc atcgatctag
      481 gctcaccaag tatggtgctt gtggccatcg caggggtcgc ttatctgctt caaggatata
      541 tttcaacaat cggtattcct gaagaacaga aaaagacgat gcgtacgatg ctgatcgtct
      601 ctcctgctat gatcgtcttc atgtcgatca gtgcgccagc cggtgttaca ctttactggg
      661 tagtcggtgg tgtgttcagc tgtctgcaaa cattcatcac aaacgttata atgaaaccgc
      721 gtttgaaagc acaagtggca gaagagttga aacaaaaccc gccaaaacaa gtcgttactc
      781 caagaaaaga cgtgacagaa tcagcaaagc cagctgacaa caaacaagca acaaaacaaa
      841 tcaaacaacc aaaaaacagt acaaacaaca aaggccgcaa cgcaggaaag cagcagaagc
      901 ggaaataaaa atatatgaca aaaaactgta gacaaataaa aaaatttctt tgttctacag
      961 tttttttgtt aca
//
LOCUS       DAFFSV010000287          943 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.294, whole genome shotgun sequence.
ACCESSION   DAFFSV010000287 DAFFSV010000000
VERSION     DAFFSV010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.294"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>943
                     /locus_tag="LR110_003016"
     rRNA            <1..>943
                     /locus_tag="LR110_003016"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAFFSV010000288          942 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.295, whole genome shotgun sequence.
ACCESSION   DAFFSV010000288 DAFFSV010000000
VERSION     DAFFSV010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.295"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          334 a          117 c          175 g          316 t
ORIGIN      
        1 aaacataagt taactaaaat tattttatta agaatgatgg aatataagga taaggttacc
       61 aacataacga tcattacttc tctgatatgc tatggacttc tatcaatcag tattttgtct
      121 atagattatg gagtaactat actgcaagcc ttagttttgg ctatgcctat gtatctatct
      181 ttgtttatca tgcatgaaat atataagcta tttgttttat caaaaaatgt agaaatacgg
      241 gtaaatattt tggtgaatag ttatcaaaaa gaactcaaag aaagaagacg gtggcggtat
      301 ttattgaaag gatgaaaaag taggatttga catactggta ttcagaaagt gccgtcactt
      361 ttaaaacaat ggaaaaggtg gagataagat gaagaaaaaa caaactaaac tatcattagc
      421 agatatttaa cacagttaac cgcaactgaa gatggtgttg ttgaatttga acaatctgaa
      481 attgcggtag tagatgatcg ttaccttaaa atgccttact tctttgacca agcaaaggtc
      541 atttgccttt gtcactacga tggggtacgt gattttaggc gttaaaaagt agtgattata
      601 tcaactttac gagtaaatat aaatttagac atgaaactga attatccaat ggaaaatcat
      661 ggtttataat ctagaaaaat tatagtaatg attggattat atatattctt tcgtattttc
      721 gtacaactgt agcttgtcaa aattgagaaa tttaaccgat taggtacttt acatcgttat
      781 ttgggtaatt tctaaaacat gaagcttacc aacgaataaa ctgagactat cttattaatt
      841 gatcaatata agaaaatatc tttgaggaga tgatgtacta tgttttcgtc tatccacgat
      901 tggttaggtg gtcttttcgg cggtggaggt aaaggttttt aa
//
LOCUS       DAFFSV010000289          919 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.296, whole genome shotgun sequence.
ACCESSION   DAFFSV010000289 DAFFSV010000000
VERSION     DAFFSV010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.296"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..132
                     /locus_tag="LR110_003017"
     CDS             <1..132
                     /locus_tag="LR110_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL4823919.1"
                     /translation="RRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            284..814
                     /locus_tag="LR110_003018"
     CDS             284..814
                     /locus_tag="LR110_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823920.1"
                     /translation="MISDNKWEINLKRFISEYIYTIETKSSFSLNEDSDPLKKYTIQC
                     KKNKDEKLFVIRPDLMKSNTHYIKEKPKDCDYIIISEANKTVFLIELKSSAQTSPAKE
                     ISEQIDGGYKWLQHLSFLTKYDFTEFSYKIIKVCCKFEARQDRKNLFKPNEYGIYNMN
                     GPKLDLRELYRKCNDL"
     gene            complement(809..>919)
                     /locus_tag="LR110_003019"
     CDS             complement(809..>919)
                     /locus_tag="LR110_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823921.1"
                     /translation="ENIVKDLNEGVSISKIAKNYNVTRQTIYRIKKDLNL"
BASE COUNT          361 a          120 c          143 g          295 t
ORIGIN      
        1 cgcagaagag aaaagattat tcgcatcttc cccaatcaaa catcagccaa tcgcttaatt
       61 ggagccgttc ttatggacct acatgatgaa tggatttatt cttcaagaaa atacatcaat
      121 tttgataagt agaaatggta aaaacattgt atagcatttt acacaggagt ctggacttga
      181 ctaaaatttc taacatcagt tattatgata attttgatga tgtcaattat gatttacagg
      241 aaagatataa tgatttaatc gaagaattag aggagagagg agaatgattt cagataataa
      301 atgggaaata aatctcaaaa gatttatttc agaatatata tatacgattg aaactaaatc
      361 tagtttttct cttaatgaag acagtgatcc tctaaaaaaa tacactattc aatgtaagaa
      421 aaataaagat gaaaaattgt tcgtaataag accagatctt atgaagagta atacccacta
      481 tataaaagaa aaacctaaag attgtgacta catcatcata agtgaagcaa ataaaacagt
      541 atttctgata gaacttaaaa gctcagctca gacttcccct gcaaaagaaa ttagtgaaca
      601 aattgatggt ggatataagt ggttacaaca tcttagtttt ctaacaaaat atgattttac
      661 tgaattcagt tataaaatta ttaaggtatg ttgtaaattt gaggcaagac aagatcgtaa
      721 aaatcttttt aaacctaatg aatatggtat ttataatatg aatggtccaa aattagactt
      781 aagagagttg tatagaaagt gtaacgatct ataaattcaa atctttttta attctatata
      841 ttgtttgtct agtaacgtta taattctttg caatctttga aatagagacg ccctcattca
      901 agtccttaac gatgttttc
//
LOCUS       DAFFSV010000290          915 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.297, whole genome shotgun sequence.
ACCESSION   DAFFSV010000290 DAFFSV010000000
VERSION     DAFFSV010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.297"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>915
                     /locus_tag="LR110_003020"
     CDS             <1..>915
                     /locus_tag="LR110_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL4823922.1"
                     /translation="NSKYSKMKDSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQA
                     DLMDKLNCYQGKVKTILNELETYNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLF
                     IQNSFDDDLSETIDKSTSSRNAEIARREETAKTLDNTGNAEIARREKTAETLDNTGNA
                     EIAQDYYINKSLDTKRHLKDTETDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKT
                     FSNTYEEAQQTVTTIHNAKHKAEELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQK
                     TEKVNNLQNLIFVYVKNWFIEKAIAAKLA"
BASE COUNT          380 a          148 c          153 g          234 t
ORIGIN      
        1 aattctaaat actctaaaat gaaagacagc tcaaaaatag cctatatgct ttttaaaaat
       61 gaatttcgta gagccttaca tgaaaaatgg atcgacgatg atggtctatt atatttggaa
      121 tttacacaag cagacctaat ggacaaatta aattgctacc aaggcaaagt aaaaaccatt
      181 ttaaacgagt tagaaacata taatttgatt tcagtcgtaa aaggagaatt taacccaaaa
      241 acgaaaaaaa atgaaaaaaa taaatactat ttacttcaac ctgaaataag tgccgatgat
      301 ctttttattc aaaattcttt cgatgacgat ttaagcgaaa ctattgataa atcaacctct
      361 tctcggaatg cggaaatcgc acgtcgagag gaaactgcta aaacccttga taacactgga
      421 aatgcggaaa tcgcacgtcg agaaaaaaca gctgaaaccc ttgataacac tggaaatgcg
      481 gaaatcgcac aagattatta tattaataaa tctttagaca ctaaaagaca cttaaaagac
      541 actgaaacag accaattaca aaatcaagtt ttgctagata acttcgttga aatcatgaaa
      601 gaagacagca taaacacatt tgtaccagaa aaggtattaa gcttgataaa aacattttct
      661 aacacttacg aagaagccca acaaaccgtc accacaatac acaacgcaaa acataaagcc
      721 gaggaacttg agaagacggt agttgtgtat gaagaacttg aatcttacgg aattgatgcg
      781 gataaaggat tatatatgac gttgctgaaa gcttatcaaa aacaaaagac tgagaaagta
      841 aataatttgc agaatttgat ttttgtgtat gtaaaaaact ggtttattga aaaagctatt
      901 gcggcaaagc ttgca
//
LOCUS       DAFFSV010000291          908 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.298, whole genome shotgun sequence.
ACCESSION   DAFFSV010000291 DAFFSV010000000
VERSION     DAFFSV010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.298"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..95
                     /locus_tag="LR110_003021"
     CDS             <1..95
                     /locus_tag="LR110_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL4823923.1"
                     /translation="EVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            218..856
                     /locus_tag="LR110_003022"
     CDS             218..856
                     /locus_tag="LR110_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL4823924.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          294 a          145 c          141 g          328 t
ORIGIN      
        1 ttgaagtacg ctcggaacat ctaaaaaatt gtacgcttaa tcagttgcaa gcacttggtt
       61 ttgctagaaa atttttacaa ataaaagtag tataatcaaa atgaatatat aagataaatt
      121 tttagcaata caaatatcca taaaaaaaca ttgcctattt taactcttca tgtatactga
      181 ctctatcttc aaaaactgaa tagtaggagt tattatcatg aataacattt taggaaaaaa
      241 aatagaactt aaaaaaggga tattgggcac tattgtcgtt gtactcggtt tgttagggtt
      301 gctatttgcg ccatccatta ttagcatttt ttctttaact atcactggat ttatagcatt
      361 tacacttgtt tttggtaaaa aagatacgaa aaaaatgttc tcgaaacctg tcgctcctgt
      421 caaaaatata gccaaatatt tttttatcaa tgtagtaatc agtgccgcag tctcggtatt
      481 tttgcaacaa attttgaaat ggggtttaac aggcaatcca ataaatgagg ctttcagccc
      541 gttattattt attattttgc ctatcatgat acttggagaa gaattgttct cagtgtattt
      601 tctcgctatt ttttctagta agttcagcat tcctgtcgct agtttcttgt cagcaatcat
      661 ctttggattt atccattaca gtacttatga taatggaaat attttacaca ctgtagcaca
      721 catccttttg atacaagggg tggcaagatt gttattcaac caagctgcca ttaaaagcaa
      781 ctcgatcatt acaagttggg cagttcatgt gatatttgat ttcacagcta tattgctagt
      841 cgttttattc tcctaaattt ttatagaaat aatacatgct ctattgctaa gaaagcatac
      901 tttatttc
//
LOCUS       DAFFSV010000292          905 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.299, whole genome shotgun sequence.
ACCESSION   DAFFSV010000292 DAFFSV010000000
VERSION     DAFFSV010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.299"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..113)
                     /locus_tag="LR110_003023"
     CDS             complement(<1..113)
                     /locus_tag="LR110_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL4823925.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSS"
     gene            complement(110..529)
                     /locus_tag="LR110_003024"
     CDS             complement(110..529)
                     /locus_tag="LR110_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBL4823926.1"
                     /translation="MEKYLNTLSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(599..739)
                     /locus_tag="LR110_003025"
     CDS             complement(599..739)
                     /locus_tag="LR110_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL4823927.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(732..>905)
                     /locus_tag="LR110_003026"
     CDS             complement(732..>905)
                     /locus_tag="LR110_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823928.1"
                     /translation="SGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
BASE COUNT          264 a          199 c          118 g          324 t
ORIGIN      
        1 gaactacctt cgtttgcgcc caaattgaat tcattgttga tcgagtaagc aaaggcgttt
       61 attggcaata aaaaaagagc tataatagct cctacaaaga ttttcttttt catttggttc
      121 ctcctcggtc tttattttca gtatcatttc gtatttgttg gaatacctct tttaacttat
      181 ctggcaaagg aaggaatacc gaaatgtttt ctaaaaaact aatcccttca tttgcaacat
      241 agaacataat aactatttct cttagaggta tagaattccc aacgattttc tgtacgtcaa
      301 ccgcaacggc aatcacaacg aagattaaga ctttcttcaa caatccttcg aaacctatct
      361 cactacttat ttctttcaat ttccatgcct tagcaagtcc agttaagaag tccacaccca
      421 caagaaaaat taaaacatgt agtatattgt ccatccctcc caataaacca acaattgtcc
      481 cgcctatcac ccctgtcatt actgagagtg tatttaaata tttttccaat atctatttct
      541 cctttatgca aagaagcgca ctcgtttgag tacgcttttt atttaacttt gtgcttaatt
      601 aaatttcatt tccagtaatc tgcttatatt ggtcttttgt taaacagttc atgtttacat
      661 agtcaagtaa atcttggtcc gtataacatc cccaatcata aaattgtttt atattttcaa
      721 agcccggaaa actattcatt acttttccct ccattcttaa tacttgcgac ttccatcgtc
      781 aattgagcta acatttgttg cattttttct tgttcctgta ccgatatcgc caattgtttt
      841 gtcagtagag catttaattg cttcaactga tcgacatctt caatatcgtc aaccgctgtg
      901 ccact
//
LOCUS       DAFFSV010000293          886 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.300, whole genome shotgun sequence.
ACCESSION   DAFFSV010000293 DAFFSV010000000
VERSION     DAFFSV010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.300"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          311 a          145 c          140 g          290 t
ORIGIN      
        1 aaatgttgat cgtcagcaag gagctaaaaa atacgaatat atttcttttg tagtaaaatc
       61 ggactggttt gcatatcatt ttcttcgtaa atatcaaaat catgttaact actatcaatt
      121 actaataaaa actaccactt taagcgttaa gaactgttaa gtttagttaa gtgaggatag
      181 ttcacaaaat ccattgttaa acttcacgaa agtggtaaaa ctcaaaatgc tttatatagg
      241 attacagcat cgctctttct tcgattgcgc gttgggtaaa aaatattatg aagtaaaaat
      301 tgacgatgac acgttcctta cggctcatca gatgaaacag ctacaaaaac gaaatgatca
      361 actcgaagaa aatttaattc taaaaaagcg attgccatat tcacgcctcg ctcgacgaac
      421 tattaaaggt caagatttac ctgaagatca tcctttttag gaaattgaag ctttttatgt
      481 tgcacaatgc atttataata ctacaatgat gttttcacca gatattcttc tagggtgcgg
      541 tgtaatgaaa cagatacact tgcaaaagaa aattcaccgc gaatttacta aaattatgaa
      601 taattatgtt aatcttccga aatttttttc caaaattaag ggatcaagct ggtgttatag
      661 attgtttaag gttggcaaaa aaataacttc tttttaaaca attatagaaa accgatactc
      721 tgttaagttg gttgacacgg tgtcgatttt tatgactttc ttattctgaa acttcactaa
      781 cggacagtac ctaaaaagtc tatagtatgg acgtttacga gtactatttt atcaaaaatg
      841 ctgctcattt caacttatat gttttagtga actaccccca cttagc
//
LOCUS       DAFFSV010000294          873 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.301, whole genome shotgun sequence.
ACCESSION   DAFFSV010000294 DAFFSV010000000
VERSION     DAFFSV010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.301"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            139..525
                     /locus_tag="LR110_003027"
     CDS             139..525
                     /locus_tag="LR110_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL4823929.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            623..814
                     /locus_tag="LR110_003028"
     CDS             623..814
                     /locus_tag="LR110_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823930.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          327 a          135 c          138 g          273 t
ORIGIN      
        1 tttgaaaata aacatcagca gtcctatcaa tgcatgtgag cttatagaag ttattcgcag
       61 cgcagcataa ttgcattagc ttcttttcgt gttttttgcc tcttttccct ttaaatctta
      121 atggaggagg tgtgcaatat gtcagataac gacgaacgcg taaatgccca gttgattcga
      181 ttctttgaaa aggtcatttt gaacaccgct aatacttatt ataaaaaaca agaagaaatt
      241 caaaaacatg aacaacttga tcaactccca ccatatttgt ttactaaaga tactataaaa
      301 tttaaacaac cgattaatgt tttaaatgtg actctccttg ttgaagatat aaaattagca
      361 aaaatccttc ctttgttgaa agaacctgaa caaatttttc ttatcgaaaa atttatcttt
      421 aataaaactg ataaagaaat tggaaaggtt ctgggtatta caagacaggg tgcgacaaat
      481 ttaaaacatc gactttacaa aaaaattcaa aaaatgctgg tttaatatct gcataataac
      541 taattttgta tgcttctttg ctcgtcctcc tccttcccaa aaaaataaat agattataag
      601 ttcatgggaa gaaggtaaat ttatgaatac tttaaaagaa gatttcattt tagcaaagaa
      661 tggaaatgaa gaagcagttg tggccattct caaaagattt agcgctttgg tccataagca
      721 atcctggaga actggaaaat atgatcaaga ttgttatcaa gaatgtatgc ttgcaatata
      781 tctagctatc tcgaaattcg aaattaaaga ataactcaac taagggagtc tgtgacataa
      841 gtaaaaagat agtttcaaat ttcgaacaat aaa
//
LOCUS       DAFFSV010000295          856 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.302, whole genome shotgun sequence.
ACCESSION   DAFFSV010000295 DAFFSV010000000
VERSION     DAFFSV010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.302"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(101..301)
                     /locus_tag="LR110_003029"
     CDS             complement(101..301)
                     /locus_tag="LR110_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL4823931.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            697..846
                     /locus_tag="LR110_003030"
     CDS             697..846
                     /locus_tag="LR110_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823932.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          281 a          146 c          132 g          297 t
ORIGIN      
        1 gttttataca ctactattca gcccattcaa cgaccaatga attttttcgt acaaaaaaac
       61 cgtcttttct ttcgaaaaga cggttaaaag tcaattttta ttaagcttta acaatgtttg
      121 ttgcttgagg gccacgttga ccatcttcaa catcgtaaga cactgcttgg ccttcttcta
      181 aagttttgaa gccgtcacct tggatagctg aaaaatgtgc aaatacatca tttccatctt
      241 caccagtgat aaagccaaaa cctttgtctg cgttgaacca ttttactgta ccgttattca
      301 taaataaatt cctcctgata cgtatcaatg tacgtttttt cgttgcaata aatatttgat
      361 aggcaaaagg agttttatct atgtagatat atcgctcaaa ttacgtttca aaatagatta
      421 ctcatttaat atatcatgct ttttctaata acacaaggat aatgcaatga aatataaaat
      481 tcattgtctt ctattgtctt cgaatcgaag aaaaagttga ataaccgcga ttgattcact
      541 ttttgttttc ttgtttattt gcttttatct gcaacttttg actttaaaat ttcttaacta
      601 tgaaaacctt tcaattggtg gtatacttat aaatggaatt tcgttatcaa cgcgttcgtt
      661 gcctttcagc aagtatttaa agaaaggaga atggctatga cattctataa caaagatata
      721 caatatcgag taaccttgga tacagatttg aatctatttg tcgtttttga caaagaaaat
      781 accaatcatg tagcgacagg tgttacgatc gaacaagcag tgcaagaatt aaaaaaatct
      841 gcataaggag tggaaa
//
LOCUS       DAFFSV010000296          855 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.303, whole genome shotgun sequence.
ACCESSION   DAFFSV010000296 DAFFSV010000000
VERSION     DAFFSV010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.303"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(475..699)
                     /locus_tag="LR110_003031"
     CDS             complement(475..699)
                     /locus_tag="LR110_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823933.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          310 a          114 c          153 g          278 t
ORIGIN      
        1 tgcaatcttt tttgctacgt aatttttgta aaaaagtggg ttgagagaaa tctaattgat
       61 tttcaaagaa ggagtatgaa aatgagaaaa atatttattc gtttattgtg ggtactatct
      121 tagaggaata aataatagtg gagaaaacta tggtttggcg cagattctat caggtttgat
      181 ggaacgaaga caacctcccg acatcaagtc aaaagctact tgagattttg atctgaaaat
      241 aaaaaaagaa aacaaggtag gaacatcaat gtttctacct tgttttcgta atcaatggga
      301 aaaaactaag agtggtgtac gtaatcaata aaaaggatgg tgacaacagt acctctttac
      361 tctctgaaag catcataact cgtttttcct cattatgcca ataaattgtt gttataccac
      421 atataaggat ttgtaatagc taaaaacaga caggaaagtt gaaatctgtg aatcttattt
      481 tgtatggaag gatttataga acgcaaaaat caagataaag atgaataaaa atcctgtttc
      541 ggctatatat tgcgtatcta cgccaataaa acgagtttgt tctgtcatag ccatataaag
      601 caaaggcaaa aatacaacta cagcgagata agatccgatg atttctcgaa aagttttcca
      661 ggaagtatgt ttcctgtttt tatatttatc cgatttcatt gaatagtcat agcctcctta
      721 ttctttcata tgttgaaatt ccagtataaa aactggaagg aactgtacgt tttttagaat
      781 aaaaattata agggataagt aggctgctgt ctataataag atatattttt caattcttta
      841 ttaaagattt tattg
//
LOCUS       DAFFSV010000297          812 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.304, whole genome shotgun sequence.
ACCESSION   DAFFSV010000297 DAFFSV010000000
VERSION     DAFFSV010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.304"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(45..117)
                     /locus_tag="LR110_003032"
     tRNA            complement(45..117)
                     /locus_tag="LR110_003032"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(82..84),aa:His,seq:gtg)
     gene            complement(120..193)
                     /locus_tag="LR110_003033"
     tRNA            complement(120..193)
                     /locus_tag="LR110_003033"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(159..161),aa:Trp,seq:cca)
     gene            complement(200..280)
                     /locus_tag="LR110_003034"
     tRNA            complement(200..280)
                     /locus_tag="LR110_003034"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(244..246),aa:Tyr,seq:gta)
     gene            complement(282..357)
                     /locus_tag="LR110_003035"
     tRNA            complement(282..357)
                     /locus_tag="LR110_003035"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Phe,seq:gaa)
     gene            complement(393..465)
                     /locus_tag="LR110_003036"
     tRNA            complement(393..465)
                     /locus_tag="LR110_003036"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(430..432),aa:Asp,seq:gtc)
     gene            complement(475..547)
                     /locus_tag="LR110_003037"
     tRNA            complement(475..547)
                     /locus_tag="LR110_003037"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(512..514),aa:Val,seq:tac)
     gene            complement(563..634)
                     /locus_tag="LR110_003038"
     tRNA            complement(563..634)
                     /locus_tag="LR110_003038"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(599..601),aa:Glu,seq:ttc)
     gene            complement(649..738)
                     /locus_tag="LR110_003039"
     tRNA            complement(649..738)
                     /locus_tag="LR110_003039"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(702..704),aa:Ser,seq:gga)
BASE COUNT          224 a          225 c          186 g          177 t
ORIGIN      
        1 aagtcccttg ccttaccgct tggctatacc ccaagaataa aaagggcgaa tgatgggaat
       61 cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca cattcgccat
      121 ggcaggggca gcaggaattg aacccacact aacggttttg gagaccgctg ttctaccttt
      181 aaactatgcc cctataaaat ggaggagagt ggattcgaac cactgaaccc taaggaacgg
      241 atttacagtc cgtcgcgttt agccacttcg ctactcctcc atggtggcgc gggacagaat
      301 cgaactgccg acacacggag cttcaatccg ttgctctacc aactgagcta ccgcgccata
      361 ttttaaaatt attaattgaa taacaagaac gacggttccg acgggaatcg aacccgcgat
      421 ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg gaaccagttc attatggagg
      481 ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc agctgagcta
      541 aacctccaac attcaattaa aatgacccgt acgggattcg aacccgtgtt accgccgtga
      601 aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa atttgctacg gagagtaagg
      661 gattcgaacc cttgagacag tttggcacca tctacatgat ttccaatcat gctccttcgg
      721 cctctcggac aactctccag agtgaaagaa gctcaatact ctttctcaaa atgagaactc
      781 cggcagtagg actcgaacct acgacatcat ga
//
LOCUS       DAFFSV010000298          803 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.305, whole genome shotgun sequence.
ACCESSION   DAFFSV010000298 DAFFSV010000000
VERSION     DAFFSV010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.305"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            5..235
                     /locus_tag="LR110_003040"
     CDS             5..235
                     /locus_tag="LR110_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL4823934.1"
                     /translation="MGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDV
                     STSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(439..801)
                     /locus_tag="LR110_003041"
     CDS             complement(439..801)
                     /locus_tag="LR110_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823935.1"
                     /translation="MKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLFA
                     GYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVPQ
                     ILGSLWAVALSYKKARHP"
BASE COUNT          324 a           96 c          146 g          237 t
ORIGIN      
        1 aagtataggt tttagaacta aacgaagtct gtcttctaaa aaaaattggg tatactcaca
       61 aactattttt tatggaggaa ttgttttaat tagtttactt tcttcaacac tttattcttt
      121 gaatattata gatgtttcca cctcaaattc aatatctatt attggaatca ttattgctgc
      181 aattattaca caactgtttt tagtgtttgg agagaagaaa agaagtaaaa aatagaaaaa
      241 ctagtgatag ttttggtgat agtgatatta aaaatcagaa aagatagaaa aagcaggaga
      301 tagataaaat gctgatattt cagcattttt taagagtata aaagagaaaa aaagtaataa
      361 atgtatacat gatataagaa aaacatatat gtataaaaag aagaaagtag cttctctttg
      421 tacatatatg gtttgttttt atggatgccg agctttttta taagataagg ctaccgccca
      481 taacgaccca aggatttgtg gaactaagta aatacctgca agaagcaaga tcagctgggt
      541 cggttttaaa ggatgataga agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga
      601 agtgacagta gatataatat tggcaatgaa taaagatata atacgcctgt tcctttgaaa
      661 ataatatcca gcaaaaagag ataaaaatat caatgtgatg aacccgctta cggaatgata
      721 acgaaaatca agcagtaagg cgaaatgcac ataaggaaca aaagataaaa gcaacggaag
      781 gtttttccag caagttttta tca
//
LOCUS       DAFFSV010000299          798 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.306, whole genome shotgun sequence.
ACCESSION   DAFFSV010000299 DAFFSV010000000
VERSION     DAFFSV010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.306"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            168..536
                     /locus_tag="LR110_003042"
     CDS             168..536
                     /locus_tag="LR110_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBL4823936.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          278 a          131 c          117 g          272 t
ORIGIN      
        1 ctttgccaag tcaatcattt ttatgtctct tttctatttt attatttgag acatcttatc
       61 atataaatat agcggttaca acattacttg tgttttgttc gttttttata cataactatt
      121 gtttttctgg taaaataaat caaaccactt aatggaggcg ttttagaatg gaaaaatttc
      181 agccgatcat caatcaaatc aaagaaaaag aacatcaaca aaagatcaaa gacctttttg
      241 ctaagataac agaaacgtat ccccagctag acacaactgt aaaatggaac caacctatgt
      301 ttactgacca tggtaccttt attattgcat ttagttttgc taaaaatcat ttttctatcg
      361 caccggaaaa agcagcgatt cgtgcccttg aaaaaaatat ccaagaagca ggctacgtat
      421 atacggataa cgtcatcaaa gttccttgga aaagtgtaat caactgggaa ttgattgaac
      481 aattgatttc atttaatatt gaagataaaa aaggtcatga taaattttgg cgataaagct
      541 ttatacgaat aaacctttta ctggcgagga aactattcat caccttatac aggttttgtt
      601 caatcctatc ctttttatca tccctggtta aacacttaat tgacaaagta tctgctattt
      661 ttataaggag tctttcttgt gctgaaaaaa atcactgatt gaatggcttt gaattttcta
      721 cttagtaaaa ttattttttc agcaattttc ctgcaaaact tttgaataag gaaaaaatac
      781 gagaaaatat cagtgatc
//
LOCUS       DAFFSV010000300          793 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.307, whole genome shotgun sequence.
ACCESSION   DAFFSV010000300 DAFFSV010000000
VERSION     DAFFSV010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.307"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="LR110_003043"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="LR110_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBL4823937.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="LR110_003044"
     CDS             complement(481..675)
                     /locus_tag="LR110_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823938.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          238 a          167 c          136 g          252 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttacttt ataaaggtcg tccaaaactc
      781 tatagtgcag ata
//
LOCUS       DAFFSV010000301          783 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.308, whole genome shotgun sequence.
ACCESSION   DAFFSV010000301 DAFFSV010000000
VERSION     DAFFSV010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.308"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>783
                     /locus_tag="LR110_003045"
     CDS             <1..>783
                     /locus_tag="LR110_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBL4823939.1"
                     /translation="VYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFL
                     TKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNKRKELDISM
                     STFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAKAVELDIE
                     KRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLT
                     NNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTE"
BASE COUNT          301 a           95 c          153 g          234 t
ORIGIN      
        1 ggtttatttt ggcgggggaa ttaatttatg gttagccaaa aagaatggaa tcagacagag
       61 aattgctcat agccatgcaa ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt
      121 gcgtaaattt ttaaccaaat tgttgttaca aaatgcaaca gactaccttg ccgttagtca
      181 agaagctggt gaaagtctgt ttcaaaacca tccctttgag atagttcata atggtattga
      241 tttagagctt tatagtaaaa attcagaagc taaggttaat aagagaaaag aattggatat
      301 ctcaatgagt acttttgttg tgggaaacat tggacgatta gagaaacaaa aaaatcaatc
      361 ttatttgtta gagatttttt tagaaatact aaaaatgaat tctgattctt acttgatttt
      421 agtaggtgaa gggtcactta gaaataaact gaaagccaaa gcagtagaat tggatataga
      481 gaaaagagta aaatttttag gagaaagaaa cgatattccc gagttattat ctgtgatgga
      541 tgtttttgta atgccttctc tttatgaagg tctgccaatg gttggattag aagcacaagc
      601 agcaaaatta aaattagtct tagcagatac tatttccaaa gatacaaagt taacaaataa
      661 tgtggatttt gtatcattag atgaaacacc acaagaatgg gcaaaaaaag taataaaaga
      721 gccgtatgct aataaaataa cacaagaact aaagagttat gatgtttcgt acactactga
      781 aca
//
LOCUS       DAFFSV010000302          777 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.309, whole genome shotgun sequence.
ACCESSION   DAFFSV010000302 DAFFSV010000000
VERSION     DAFFSV010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.309"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..311)
                     /locus_tag="LR110_003046"
     CDS             complement(<1..311)
                     /locus_tag="LR110_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823940.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VT"
BASE COUNT          228 a          185 c           94 g          270 t
ORIGIN      
        1 gtaacctcct cataatcagt tagtactata aaggtatctt tgtttttatc caaaaaatct
       61 aataaatctt ttgtagaaga tatctcttca tcaacttcac cataataaat accttcgata
      121 attgttgcta tatctgaaaa actaattttt actggacatt tatcgtctac cgcattcaat
      181 accgacaaaa ttgcttcttt ttctgcaatc tctcgtttga atttctttgg agctaatcgc
      241 atgacactca agtcattttc aagcttttca atccatgcag ttttactcat tttcgcaaat
      301 ttttgaatca ttcctctacc acctttgcta tctactaaat tagttttact tcataacgaa
      361 tatctttatc gtttttcgtt cactcaaaaa tatataaaat aaatcgactc gtttatggcg
      421 tccgaaacga gaaaccctag tagacagcgg taaatgctcg attgatccaa tgatcaacgt
      481 cctcgcaagt atctacatgt actgcagcac acgacacaat ttcttgtgcg tcctccccat
      541 tacgagaaga cctactcctt attccatgag tttgcgtcaa agcctagatc ccttagtagc
      601 tgctcatgat taatctaggc gctattctaa cttgtctaac ttctaaattc cctcttgttt
      661 actctgtgca tattatttac acaaattcat ttcaccacta tccaaacaag ggcatttact
      721 cgattgacca attttttacg ttaccaatcg taaaaaccta ccccgtcact ccccgaa
//
LOCUS       DAFFSV010000303          767 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.310, whole genome shotgun sequence.
ACCESSION   DAFFSV010000303 DAFFSV010000000
VERSION     DAFFSV010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.310"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          223 a          118 c           97 g          329 t
ORIGIN      
        1 atatgctagc cactaaattc atcatatttg attccttttt taagataatg tagcatcttt
       61 ttatccaata acgcaattgt ttttaaggat aaatttctta agatggtaca tttcaaaatg
      121 gcatttgaca tatgaaacaa aaaagaaaga aaagagcata agggaaaact gatagcacct
      181 tcctacagga aggtggctaa gttggctgtg ccaactggtt ttctttcaaa atcatttcat
      241 aatttttgat atcataaaaa aatccatttt ggacctattt tcggtcataa tttagtacct
      301 acttttggtc atagtttcgt ccgtagtttt ggtcatagtg actaaggctt ttctgttcac
      361 aaaattggtt taaatttgct ttctttaaca cttcatttat atcatagcaa ttataatgtt
      421 tacacaacat cattcctaat tttaacggtt gttcctcttt atatttttta agttctacat
      481 ttttttgcgt ttaattactt ttctcttttc tttatattcc gctccaactt tctctttaat
      541 agtgataata gaattacata tttttgttaa acttccttaa aaaaattgga tctcgattac
      601 tcaaatttgc ttgcaggctt ttctaattgc tttgtttttt cttcctaatt atttgatttt
      661 atttccattt tcttctagtg aaaatttatt atttttaata atcgtacgcg tagaactgat
      721 ttcatttttt gtatctgttt gattatctaa cgtttgaaat tcaaagt
//
LOCUS       DAFFSV010000304          767 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.311, whole genome shotgun sequence.
ACCESSION   DAFFSV010000304 DAFFSV010000000
VERSION     DAFFSV010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.311"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>767
                     /locus_tag="LR110_003047"
     CDS             <1..>767
                     /locus_tag="LR110_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBL4823941.1"
                     /translation="GTLELKVPRTRDGHFSPTVFERYQRNEKALMASMLEMYVSGVST
                     RKVSTIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVRE
                     ENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKERGLQGTELVISD
                     AHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAR
                     EAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQG"
BASE COUNT          268 a          146 c          144 g          209 t
ORIGIN      
        1 taggcacgct agaattaaaa gtacccagaa cacgtgatgg ccatttttca cccacagtgt
       61 ttgaacgtta tcaacgaaac gaaaaagccc tcatggcttc aatgttggaa atgtatgtat
      121 caggcgtttc aactcgtaaa gtatcaacaa ttgtggaaga actttgtggt aaatccgtct
      181 ctaagtcctt cgtttctagc ttaacagaac agctagaacc tatggttaac gagtggcaga
      241 atcgtttatt atcagaaaaa aattatcctt acttaatgac cgatgtactc tatataaaag
      301 tacgagaaga aaatcgagta ctctcaaaaa gctgtcatat agcgattgga ataaccaaag
      361 atggcgaccg tgaaattatc ggcttcatga ttcaaagtgg cgaaagcgaa gagacctgga
      421 caacattttt tgaataccta aaagaacgcg gtttacaagg tacggaactc gttatttctg
      481 atgcgcacaa aggattagtc tctgccatta gaaaatcctt caccaacgta agttggcaaa
      541 gatgccaagt tcacttccta agaaatatct ttaccaccat tcctaaaaaa aattcaaaat
      601 ctttcagaga agctgttaaa ggaattttta agttcacaga tattaactta gcgcgtgagg
      661 ctaaaaatcg attgattcat gattatatcg atcaaccaaa atattcaaaa gcttgcgcat
      721 cattggatga tggattcgaa gacgcctttc aatataccgt acaagga
//
LOCUS       DAFFSV010000305          758 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.312, whole genome shotgun sequence.
ACCESSION   DAFFSV010000305 DAFFSV010000000
VERSION     DAFFSV010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.312"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..104
                     /locus_tag="LR110_003048"
     CDS             <1..104
                     /locus_tag="LR110_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL4823942.1"
                     /translation="GTVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            104..724
                     /locus_tag="LR110_003049"
     CDS             104..724
                     /locus_tag="LR110_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823943.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          277 a          116 c          172 g          193 t
ORIGIN      
        1 ttggaactgt agaattcccg attgaagaag gagccttgat catcgcaacc gttgatgatc
       61 caaagcaaaa tgtgttcact cttgagaaag tcggggtgat ctaatggcta aaactaaacg
      121 aatgatggaa acggatgaaa aaacaggtgt acaacgccaa ttctttccaa tcacacatgc
      181 ttccgcagtt cttgggttag aagaaataat agcaggagaa gcaacagttt tatctgttaa
      241 tggaaaaatt ggagccgtcg tcattactaa agaagatttg ggattagaga atgttctcac
      301 agaattaccc tatgcaagtg aagaagatga cggcattatc acggcagaaa tgtatcaaaa
      361 aattttaaac agtggagaag gtgactacgt gttgccagtt gccactatcg accgtttggg
      421 tggcataaaa gttggtgaat tattgacgat tgatgagaca gggaaagtct ctgcagtcag
      481 acaatctgat gtcaattttt cgctggagtt aaaagaaaaa ctcgattcac tgaaaaatta
      541 tactgctgga gaaaacatca ctattgatga agatggaaca attagttcta caggtggtag
      601 tgggactggt ggtgtcaatc agtcatatgt agatcaaaaa ttccaagaag ctgtaaatca
      661 agcagaaaat tacacaaacg aaagaattcc aaattttact tttgaaaaaa ttggggaggt
      721 atagagaatg acagatatcg taaaaataaa acaaagca
//
LOCUS       DAFFSV010000306          755 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.313, whole genome shotgun sequence.
ACCESSION   DAFFSV010000306 DAFFSV010000000
VERSION     DAFFSV010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.313"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..104
                     /locus_tag="LR110_003050"
     CDS             <1..104
                     /locus_tag="LR110_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL4823944.1"
                     /translation="ETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            104..721
                     /locus_tag="LR110_003051"
     CDS             104..721
                     /locus_tag="LR110_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823945.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          283 a           95 c          162 g          215 t
ORIGIN      
        1 ttgaaacagt agaatttcca attgaagaag gagctttgat tattgcaact gttgatgatc
       61 caaaacagaa tgttttcact cttgagaaag ttggggtgat ttgatggtta aagtaaagag
      121 aatgatggag actgatgaat ctggtgttaa acgtcaattt atgccaatta cacatgtttc
      181 tgcagtttta ggcttagaaa aaatgatatc aggacaatca aaagttcttt cggttaatgg
      241 taaatatggt gcagtcattt taacgaaagc tgatttagga ttagagaatg caattactga
      301 actaccttat gcaagcgaga caagcgatgg tattcttact gctgaaatgt ttcaaaagat
      361 cgtaaatgga gaaggtggaa catatatttt gccgattgct actcctgaac aattaggagg
      421 tataaaagtt ggggaactct tagaagttac tgaagaaggt gttttatctg caactaagca
      481 aacagacttc aatttctctg aagaattaaa gggaaaatta gaatctctaa aaatattaaa
      541 agcaggtgca aatatatcaa ttgcagaaga tggaacaatt agttctacag gtggtagtgg
      601 gactggtggt gttaatcagt catatgtaga tcaaaaattc caagaagctg taaatcaagc
      661 agaaaattac acaaacgaaa gaattccaaa ttttactttt gaaaaaatcg gggaggtata
      721 gaaaatgaca gatatcgtaa aaataaaaca aagcg
//
LOCUS       DAFFSV010000307          752 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.314, whole genome shotgun sequence.
ACCESSION   DAFFSV010000307 DAFFSV010000000
VERSION     DAFFSV010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.314"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            363..731
                     /locus_tag="LR110_003052"
     CDS             363..731
                     /locus_tag="LR110_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBL4823946.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT          244 a          117 c          157 g          234 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tt
//
LOCUS       DAFFSV010000308          732 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.316, whole genome shotgun sequence.
ACCESSION   DAFFSV010000308 DAFFSV010000000
VERSION     DAFFSV010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.316"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..451
                     /locus_tag="LR110_003053"
     CDS             <1..451
                     /locus_tag="LR110_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBL4823947.1"
                     /translation="KFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSVIYF
                     ALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFMKVA
                     DLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            460..687
                     /locus_tag="LR110_003054"
     CDS             460..687
                     /locus_tag="LR110_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4823948.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          243 a          117 c          134 g          238 t
ORIGIN      
        1 aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac ttttttatac
       61 tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat tatttgtttc
      121 tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat tgaaactatt
      181 gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac taaaaaaaat
      241 cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta aatttatgaa
      301 agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg tcgtattcat
      361 tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt
      421 acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat cgttaattta
      481 gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt
      541 acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg tttttccact
      601 ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac ctataaaaat
      661 gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa taaaaaataa
      721 tctgaatgat ag
//
LOCUS       DAFFSV010000309          709 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.318, whole genome shotgun sequence.
ACCESSION   DAFFSV010000309 DAFFSV010000000
VERSION     DAFFSV010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.318"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(131..685)
                     /locus_tag="LR110_003055"
     CDS             complement(131..685)
                     /locus_tag="LR110_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL4823949.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          210 a          145 c           72 g          282 t
ORIGIN      
        1 ttgttctatc tctggtattc tattgtaaat gttactaaac gttttaaata cttccgaaaa
       61 ttcagttatc ttcattaatc tcaccttcta agttttcatt atacacaaaa aatatatcct
      121 aatatctcaa ttagcctccc aatttaaaac cacgtaacga tgcccctatt tcatcttcag
      181 ttataccaat gtaccttttt gtaattgctt gcgagctatg attaaaaata aacattaagg
      241 tagcaatgtc tctagttttc ttgtagtagt gatacccaaa agtttttctt aatgtatgtg
      301 ttcctatatc atctcttcct aatttttctg ctatatctgc atatatctga tagacacgat
      361 cccttgttat atgtcgacct tgttgccgac ttggaaataa ccaatcattt tcttctttac
      421 cttcagtata taataaaatc tcttctctca aattatctat aaaaatcggt tttcgcttac
      481 ctgttttatg ttcaacaata ataggcttac ttttacctcg tatgtctcca acttttaatc
      541 ctataatatc tgacatacgt aacccattat tgattccaaa caaaaataaa aaactatctc
      601 tttctggatt acttgttctt cttagccaaa actgaaaatc ttgtatctct tcatttgtcc
      661 ttaacggttg tacattatag ctcactattg tttcccttct taaaaatat
//
LOCUS       DAFFSV010000310          708 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.319, whole genome shotgun sequence.
ACCESSION   DAFFSV010000310 DAFFSV010000000
VERSION     DAFFSV010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.319"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            312..608
                     /locus_tag="LR110_003056"
     CDS             312..608
                     /locus_tag="LR110_003056"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL4823950.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTVCMKKLDVC"
     gene            complement(531..708)
                     /locus_tag="LR110_003057"
                     /pseudo
     CDS             complement(531..708)
                     /locus_tag="LR110_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          239 a          133 c          103 g          233 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccacggt ctgtatgaaa aagcttgacg
      601 tctgttaaag aatacggtat ccgtccaaag gcttctttta ccaatgaggc atctttcttc
      661 tcaccacagg aataaccaat aatttctcga ttaaacaaat caagtatt
//
LOCUS       DAFFSV010000311          696 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.320, whole genome shotgun sequence.
ACCESSION   DAFFSV010000311 DAFFSV010000000
VERSION     DAFFSV010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.320"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..583
                     /locus_tag="LR110_003058"
     CDS             <1..583
                     /locus_tag="LR110_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBL4823951.1"
                     /translation="GIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRD
                     ALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRIT
                     VKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGF
                     SDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          244 a           83 c          128 g          241 t
ORIGIN      
        1 tggaataatt ccaagtgtta gtggagatac gattcggtct gaaaaaaatt attctatcat
       61 tgtttttgaa aagttagcac aaacgtctat aacattagga atggatataa tagaagcgta
      121 tcaaagtaga gatgctttga tacaagaaaa cgaattagct gtatcgttac ctgaggtctt
      181 aaaagtgagg gattctggga ttgtttatta tactaaggaa atcggaaaaa ctaagataga
      241 gcatctttct cccttaattt cttctgtcgt tcaattcatc ggcttaaata tctataaaag
      301 aattactgta aaggaaatag ctaattattt ttctgtaagt gaaactaaat taagagaaag
      361 ctttaagaat gaaatgcata ttacgattta taattatatc tctaagcgaa aaatttctac
      421 ggctaaaata atgttgaaat caaaccatac tatcagtgaa gtttctttag gtttaggatt
      481 ttcagattct tcacactttt cgagagtatt caaaaagtac gcaggtgtat ctcctaagca
      541 atatcaatta ggtttagtgg ataatttcaa caacattagc tgagaggttt tattttatta
      601 tgagactata tcatttacgt agtttggagg agttgaggaa attggatttt aacaaaaaat
      661 tttttgggaa atttctgatt tgagttgttt cgtaga
//
LOCUS       DAFFSV010000312          692 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.321, whole genome shotgun sequence.
ACCESSION   DAFFSV010000312 DAFFSV010000000
VERSION     DAFFSV010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.321"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..421)
                     /locus_tag="LR110_003059"
     CDS             complement(<1..421)
                     /locus_tag="LR110_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HBL4823952.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPN"
     gene            complement(426..611)
                     /locus_tag="LR110_003060"
     CDS             complement(426..611)
                     /locus_tag="LR110_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL4823953.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          210 a          136 c           85 g          261 t
ORIGIN      
        1 aatttggtga taatggttgc tcaatgccct tttttacaac atggattttc gataaaaact
       61 gttgaaattc ctgtatatga attggtcgaa tctttacaac ctgcatttct ccaaaaaaat
      121 ccaatgcacg ttgtaaaata tgatctgttt tcatatatgt tttttctaca gttcccaact
      181 tataccaagg aaaaaaatac tctcgataaa aatcatgaaa cgttattcct tgcagataaa
      241 ttccttgtga atcagaaaaa ctttgtttta actcttcgag tccccttcta gcttctcctt
      301 gagttttgaa gcctcttctg gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag
      361 ttgcagaaaa aaaccaagtg cctctttttc tgtctttata aacattcgca attttcctca
      421 ttttactatt cctcccaaga aatatggaga acttcttcta aaattgaaat aggaattctt
      481 tttatttttg aattacgata aaaaaagtat cctttctcaa ctaaaagttc tttacattcc
      541 ttgaagattt tattagcata gtactgagaa aaacctagat taatcaaatc ttttcgagta
      601 gctgttttca ttatagcacc ttccttactt tttttcattt atttctatca gctgcttttg
      661 tcaaaagcac ataggaattt cacctctccc ca
//
LOCUS       DAFFSV010000313          683 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.322, whole genome shotgun sequence.
ACCESSION   DAFFSV010000313 DAFFSV010000000
VERSION     DAFFSV010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.322"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(27..>683)
                     /locus_tag="LR110_003061"
     CDS             complement(27..>683)
                     /locus_tag="LR110_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HBL4823954.1"
                     /translation="QISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENK
                     IPIYFYFLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKN
                     GERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKIT
                     AYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM
                     "
BASE COUNT          211 a          106 c          122 g          244 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttg
//
LOCUS       DAFFSV010000314          662 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.323, whole genome shotgun sequence.
ACCESSION   DAFFSV010000314 DAFFSV010000000
VERSION     DAFFSV010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.323"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..515)
                     /locus_tag="LR110_003062"
     CDS             complement(<1..515)
                     /locus_tag="LR110_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL4823955.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMG"
BASE COUNT          257 a          119 c           83 g          203 t
ORIGIN      
        1 atccccattg cttttgccat taatggatca aaagaagtga ttaataattc tttaaaaaat
       61 agaacaactg taaccaacac taaaacagaa acgccaattg taatccactt atcaatatct
      121 tgtacagcta aaatatttcc aaataagata tgaaataaat ccgttgagct atttgcaact
      181 cctataagga taactcctaa tgctaaaaaa gaactgaaag taataccaat tgctgtatct
      241 ccttttatta cactattgct ttttataaaa gtaataatga tagaagctaa tagaccaaat
      301 acaatcgcac caataaaaaa attaatacct aaaatataag aaattgctac tcctggcaaa
      361 acagcatgag aaatagcatc acccataaga gacattcctc ttaaaataat aaaacaacct
      421 actgttccag aaacaatccc tataactaca gatgtaatta gagcattctg taaaaagtga
      481 aagtcataca gaccattaat aaaattttga agcatattat tccccttgta tattagagtt
      541 agggttttaa ccttccggtt ttgattggat aaccctatga accatcgtta ttttaatttg
      601 gtattaactc atttcttttg agataatata tttattggat tgagaggcta agccgttccc
      661 cc
//
LOCUS       DAFFSV010000315          656 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.324, whole genome shotgun sequence.
ACCESSION   DAFFSV010000315 DAFFSV010000000
VERSION     DAFFSV010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.324"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            119..640
                     /locus_tag="LR110_003063"
     CDS             119..640
                     /locus_tag="LR110_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL4823956.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT          249 a          109 c          128 g          170 t
ORIGIN      
        1 taaaaatatt ttacatgtat ctatttgaat tggtcatcta atttttagac aaacagtcta
       61 aatcgttgcc tttttttgtc agacatgtat aaactataag cgaaagaggt gttttaacat
      121 ggatatatca gtgattgatg caacgaaaac aaatacacaa aaaggaatcc tttatggttc
      181 atcgaatgct cctaaaaaaa tggtcgaatt catcaactta gcttgtccgt attgccgaca
      241 atggtttgaa gaatcttatg agctattaga agaagcagtc caaagcgggc aattgcagcg
      301 agtgatcaaa ttgttcgata aagaaaaaga aagcttacag cgagggaacg tgatgcaccg
      361 gtatctcaca atcagcgatg gccaaaaagc gatcaaagag atcaaacaaa tattcgatac
      421 tcaagacgag tggaaacatc tttctctcca agaagtagcg gattttgcag tagataaact
      481 aaagctgaca gaacaaaaag acgaacagct gagccaagct gtcatcaacg aagctgagca
      541 agcacatatt cgatttgttc caactgtcat cttgggtaaa gaaatctttg atgaatctat
      601 ttcaatagag gaattaaaag aattgatcca aacaaaataa aaaaataagg agcgtg
//
LOCUS       DAFFSV010000316          655 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.325, whole genome shotgun sequence.
ACCESSION   DAFFSV010000316 DAFFSV010000000
VERSION     DAFFSV010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.325"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..449)
                     /locus_tag="LR110_003064"
     CDS             complement(<1..449)
                     /locus_tag="LR110_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL4823957.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTK"
BASE COUNT          228 a          105 c           95 g          227 t
ORIGIN      
        1 attttagtga gggaacagtc tacaaaatct gcgcttgata agacagagtg ctcaaaggta
       61 ctttctttta tgtttgaacc agataaattt acatagtcca ttttacaatt ctcgaataac
      121 gtgttcccaa tataactttc catcaattta gtacctgtta atttacaatt gacaaatttc
      181 acgcgatgca acatgctttt tgtcaatata cagcctgaaa aatcacagtt ttcgaatata
      241 cagtcggtaa tctccattct ttgaaaatca ttggaagcaa aaaatgcata cttgaaaata
      301 gatttgtaaa taattggatg gcagattatt tggttttcga tgacctcttt ttgtgaaagg
      361 atattataca gttcaaaatc ttcgttttca tagatatgaa aaaaggatcc tttattcaag
      421 cttgcttcat taactttagg aaaaatcata tttaattacc tccattttca taaagtcata
      481 ttaacaaaag ctaaaccaac ttacattagc agaaaatgag ataggttttc gcctttttcg
      541 ataacgatag tgtcgctatc tcttgaaacg ttcttagaat tgccctgaga gacacaaatt
      601 aggcgtaatt ctaataaaaa tcttagaata aaaattttta ctgatttaat ttaga
//
LOCUS       DAFFSV010000317          652 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.327, whole genome shotgun sequence.
ACCESSION   DAFFSV010000317 DAFFSV010000000
VERSION     DAFFSV010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.327"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          215 a          113 c           86 g          238 t
ORIGIN      
        1 gtactattca ttgatcataa cgattggcta tttgattgta tcaaatctag gaaatatggt
       61 atttggtatc agtaaagaat tcagttggac tactacatta tgggaatctc tattcttttt
      121 catcttcgta tttttgttac aaaattatcg aaaaaaataa acatgtgcca tctatctgtt
      181 ggcacatgtt ttaggattta tggtactttt tattcggcaa tccattttat ttttctactt
      241 cttcttaata cctctaacac ttttgattta taaactaatc caatgcatct acttgcgctt
      301 attaagctat ttcagctaga tagtaccacc ccatgcgtca atgcttgatt ttccttttgt
      361 ataacattgt ataagcaata aaggacaaat aagtggtgat tacaccaaac cattgaataa
      421 gaaaaattct gatacgtaaa taaacatgaa cttgaaaaaa ttaatgaatc ggttagctac
      481 tacgtagttg tcttttttct catacagttc tgttaactta cacttctctt cgctagataa
      541 taaaaaatcc tccaactgta ttgtccaata taatcccagg catctaaact agcattatag
      601 aagagacaca catagaatct aaaataactg gttttaaatg tttcttacaa tc
//
LOCUS       DAFFSV010000318          649 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.328, whole genome shotgun sequence.
ACCESSION   DAFFSV010000318 DAFFSV010000000
VERSION     DAFFSV010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.328"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..>649)
                     /locus_tag="LR110_003065"
     CDS             complement(<1..>649)
                     /locus_tag="LR110_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBL4823958.1"
                     /translation="NFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREV
                     LPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKE
                     VILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTD
                     VNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLG"
BASE COUNT          241 a          113 c           81 g          214 t
ORIGIN      
        1 taccaagaaa cgcataattc actttacttc taaaaaatgt tttgagtaaa ctaaagatat
       61 ttaacacata gaaagcatag atacatataa aagcaattat accacttgat acaagtactt
      121 gaagaaatcc attatgcatg tttccatgtg ctctttttag ttcattcaca tctgtagtat
      181 caagatctac ttttttcaaa aattttgcat cgctattccc atagaaatcg gtctctccat
      241 aaccaaataa caaagttggt cttataactt taagcgcagc tttccaaatc gtaaaacgac
      301 cagctgaaat ttcggagtta tcactagaat gctgtaatat tacttctttt tctggaagtg
      361 gagttttatc tgattcattt gactctataa cttctacttt tctatcaatc gatacaggaa
      421 ttgttgctaa aaaatcctta gcataattag taccaaaaga aaaatatacc aatagaacta
      481 ataatatgct taccatccat ttgattttta caggtagtac ttctctaatg ctctttctaa
      541 attttgaatt ggcaaacaac aaaagaaata atactaaaaa agaacaaatt actacctggg
      601 cacctctaga cgaagaaagt gtaatataaa atatttgtac gataaaatt
//
LOCUS       DAFFSV010000319          648 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.329, whole genome shotgun sequence.
ACCESSION   DAFFSV010000319 DAFFSV010000000
VERSION     DAFFSV010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.329"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..394
                     /locus_tag="LR110_003066"
     CDS             <1..394
                     /locus_tag="LR110_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL4823959.1"
                     /translation="HYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLADSN
                     EGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDAEIG
                     KILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            388..633
                     /locus_tag="LR110_003067"
     CDS             388..633
                     /locus_tag="LR110_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823960.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
BASE COUNT          247 a           96 c          111 g          194 t
ORIGIN      
        1 acattacaat gacatatcta tacaacacca atttgatgcc tactgtaaaa aagtattacg
       61 aaacgaagct aagtctattc gaaaacgaaa cgctaacata aaggataatg aggaaccatt
      121 ggcagattca aatgaaggca ggtattccca aactcatttt gatgaacaag atgtctattt
      181 cttatttgga atggaaatat taatttcaga tcaaaagtta tcagcagcaa ttgatcagtt
      241 atcggagata agaagaaaga ttatcctact ttattatttt gcaggtttta atgatgctga
      301 aatcggtaaa attctcaata tgagcacaag tggtatctgg tatcaacgaa agaaggcagt
      361 tgaacaattg aaaatggggt atgacctatg gtagataaat ctaaaaagcg gcagtaccta
      421 ttagttccac tatccattat cttatctgct ctagaaggag atccaatttc gatcaagatg
      481 atactcaaac attatcattc ttatatggct aagttatgtt tgacaaatgg atttaacgag
      541 gtaggtcaat tcgttaccta tgtagatgaa tatatgataa gtcagctaga aattaaatta
      601 atagaagcga tcttaaaatt taaaattaac taattaaaaa aatcccct
//
LOCUS       DAFFSV010000320          641 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.330, whole genome shotgun sequence.
ACCESSION   DAFFSV010000320 DAFFSV010000000
VERSION     DAFFSV010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.330"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..397)
                     /locus_tag="LR110_003068"
     CDS             complement(<1..397)
                     /locus_tag="LR110_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL4823961.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQ"
     gene            complement(473..619)
                     /locus_tag="LR110_003069"
     CDS             complement(473..619)
                     /locus_tag="LR110_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBL4823962.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT          244 a          109 c           69 g          219 t
ORIGIN      
        1 actgttcaac aatcctattt atagctaatt ttctatataa ccaaataaac atcagcaaaa
       61 acaaagaatc aagcattatc tggaatacta cataaccata cccttttatc ccttcaaatg
      121 aaaacatata cgtcataacc atggaagtaa taatagatct gaaatcatca ataagcatac
      181 ataatagtaa tttatttaaa agcactatat tagttttctt cttttccttt aatataaaat
      241 aacttccgat tataggtata gctgcggaaa catcagtaaa taattcaaca aaaaaagtga
      301 gtggtatcaa taaacataca aaaagatatt ttcttttgtt aagatcagta ttagtcatat
      361 aaaataagag aaaaaagaaa aagtttccca cgaacattct taatagataa gcttttgtat
      421 cgatagtaat caagagatcc cctcctgatt ttaggatatc ataaaaaaaa ttttagttac
      481 actttttaaa taatgaaaaa acacattcca ctaaatttga tgccggtttt ccttgaggtt
      541 ttgttccagc acctccttta atttttttaa gctcttgatc tgaacattgt ttagcattta
      601 gtctattttt ttcttccatt acgattcccc ctacaaacta t
//
LOCUS       DAFFSV010000321          638 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.331, whole genome shotgun sequence.
ACCESSION   DAFFSV010000321 DAFFSV010000000
VERSION     DAFFSV010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.331"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(75..>638)
                     /locus_tag="LR110_003070"
     CDS             complement(75..>638)
                     /locus_tag="LR110_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL4823963.1"
                     /translation="SYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWII
                     DTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIG
                     EKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTL
                     LEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          215 a          117 c           73 g          233 t
ORIGIN      
        1 ttttccatag cttattagat tttttagatg tctctttcca attgacccta acccaaaaaa
       61 aattactttc atcattaatt tacctcaatg tcgttaatta agttaataat atacttatct
      121 ttttcaaaag tatattgggg ttccattttc ttttccaaaa gcgtgaaaaa ttctttaatc
      181 tcctctaaat atgcatcttc aataatagta tgtgaataat tattatcatg cataacctca
      241 tcgtacaatt gaatatctgt catactagaa gtttcttcat tccatgaaaa tagagaatcc
      301 gggttacctt cccattctat taaaatcttt tcaccaatta tagttaaatt tcttttagct
      361 actcttgaaa caacatctac acttaaagtt ccaactatac cagtttcatg ctcaataata
      421 agagtgaaac tgtccgaata atcaatatca agattggata atttctgggt cattacagta
      481 aacgatttaa ctttcccaaa tgtatctata atccaaggta attcaatagc gaatatttct
      541 ctacacccat tagttttttt gtttgaaaca aaaaaattgc gataatcttc ccaaggatgc
      601 cagtcgggta aatactgtcc tacatggtat tgataaga
//
LOCUS       DAFFSV010000322          630 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.332, whole genome shotgun sequence.
ACCESSION   DAFFSV010000322 DAFFSV010000000
VERSION     DAFFSV010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.332"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(143..373)
                     /locus_tag="LR110_003071"
     CDS             complement(143..373)
                     /locus_tag="LR110_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823964.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
BASE COUNT          231 a          104 c           82 g          213 t
ORIGIN      
        1 gaatagtaga gattaattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc
       61 cacaccttat aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgc
      121 gtctcattaa aaagcataac cattactccg atctattttt aaacttagtc tctacctcta
      181 atatatatcg agtttttatt ttgccttcag agaatactgt ttcttttttt gcagttacag
      241 gttgtttatt ttcggaaaaa gctaatatag ctaaaattga aacatccatc tggaatttgt
      301 ctttttcgtt gctttgctca taaaaatctg catattcatc actaatattt tttctaataa
      361 attcttccat cataaagatc acctcgaaaa gagtatacga acaaacgttc tttttgtaaa
      421 ggtggaattt attatactaa ataaataggt gaacaagtac ttgtgccaaa ttatgtgcca
      481 aaaaaaatcg aatttaataa aacacaaaca aaaagaaatc ctattatgat aacatttctt
      541 ataataacaa acacaacaga agacgtgtaa tagttagtca ggaacgtaca aataacccct
      601 gtatcctttg cggtacaggg ggttattttg
//
LOCUS       DAFFSV010000323          615 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.333, whole genome shotgun sequence.
ACCESSION   DAFFSV010000323 DAFFSV010000000
VERSION     DAFFSV010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.333"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(350..422)
                     /locus_tag="LR110_003072"
     tRNA            complement(350..422)
                     /locus_tag="LR110_003072"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(387..389),aa:Lys,seq:ctt)
BASE COUNT          207 a          110 c           97 g          201 t
ORIGIN      
        1 ctctctgatt gttgtattaa tggaaaaggg gaaagcagca agtaaattta gtttttctat
       61 taaatattct tcacttttta accttttata gtacttagat ttaatcctat atgtttagag
      121 agttcattgg cagcatgatt ttgcaaagct tctcgaatta ttgttatatt agaaacttta
      181 ctgttcataa ttatgcttct tttttaactc aaagtccaaa gggctacagt ggactttgag
      241 ttaaaaataa aaagccctag ttattttgaa aaataaaaaa agaacagcgt aaatgttttc
      301 gaaatgcttt cgcaccaaca tttacctgtt ccgtgggttt ggcacccaat gggccgtgag
      361 gggctcgaac ccgcgacccg ctgattaaga gtcagctgct ctaccaactg agctaacggc
      421 ccgtacaaaa agtacttaat aatagtagca ctaagaattt taaaatgcaa gactacattt
      481 aaagaaaatc agaaaaatct ttacttcctt ataggaaatc attcatagat tattcacatt
      541 tgctcgctat tatataagca taccaacaaa caaagttctt gcaagagaac aaaccctaac
      601 acttttttca ttttt
//
LOCUS       DAFFSV010000324          612 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.334, whole genome shotgun sequence.
ACCESSION   DAFFSV010000324 DAFFSV010000000
VERSION     DAFFSV010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.334"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>612
                     /locus_tag="LR110_003073"
     CDS             <1..>612
                     /locus_tag="LR110_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL4823965.1"
                     /translation="FLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRF
                     NYLMLLINYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLE
                     GNELSIRLYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTL
                     FILRNKQQAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRP"
BASE COUNT          225 a          111 c           71 g          205 t
ORIGIN      
        1 aatttttatt gattgatgaa tttatttatt ctaatcataa aggagagatc caaaccaaag
       61 aaaccgatca aactaaaaag ttagctgttt tttctaagtt aaaattatgc tacctaaaag
      121 aatccaatcg ttttaactat ttgatgttac taatcaatta tccacaaact gctgtaccaa
      181 aaaaatattt acttgaacaa ctttatatat cgccctccta tctcgaaaaa ctgacaaaac
      241 agctcaatca ttccttaaaa aaattccatt tacggatcct atctgtaaaa aattgctgta
      301 ctcttgaagg aaatgagtta tctatccgtc tctatcttta cttttttctt tcagatacat
      361 ttggaggtaa cgattggcca ttttcctcac ttgaattaaa tgcactaaaa atgaacagta
      421 cagatgagca aatagaaaaa atttcaaaac aagaaaaaaa tatctacttg ctgttaactt
      481 tattcatatt acgaaacaaa caacaggcat attttccaaa aacttcttct gaaatcactg
      541 aattgatgga cttattgcag catacacatg attattcaga atactttact tctttttcag
      601 tgagtcgccc tt
//
LOCUS       DAFFSV010000325          597 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.335, whole genome shotgun sequence.
ACCESSION   DAFFSV010000325 DAFFSV010000000
VERSION     DAFFSV010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.335"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            227..597
                     /locus_tag="LR110_003074"
                     /pseudo
     CDS             227..597
                     /locus_tag="LR110_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          238 a           98 c          104 g          157 t
ORIGIN      
        1 aaggttatca gatataattc tttaggtttt gtaatgttat ttatatatag ctagactatt
       61 attgaaatat ttattgatta ctaattggat agatgtcaat aaagtagaca caaaaagaga
      121 agatacagac tgtagacaaa gtccatttta tgtgaatcgg ctacactaga ctagacagaa
      181 aaaataaggt gtgtagaata ataaaaaaca cactggagga tttttcatgt caagacgtca
      241 acgaagaacc tattcaaaag aattcaaaca acaaatcgtc aatctctatc tcgctggtaa
      301 gcctcgcgca gaaattattc gagagtatga gcttacgcct tcttctttcg ataaatggat
      361 gaagcaagca caatcaacgg gctcattcaa aaaaagagac aacttaacac cagaacaagc
      421 agaattgatc gcactaagaa agaaaaataa gcaactcgaa atggagaatg atattttaaa
      481 gcaagcggcg ctgatattcg gacgaaaaga caagtaattg atgccaacaa gcataaatat
      541 tccatatcag cgatgtgcaa aattctaaat atttctcgtc agacctacta ttatcaa
//
LOCUS       DAFFSV010000326          595 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.336, whole genome shotgun sequence.
ACCESSION   DAFFSV010000326 DAFFSV010000000
VERSION     DAFFSV010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.336"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..580
                     /locus_tag="LR110_003075"
     CDS             <1..580
                     /locus_tag="LR110_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL4823966.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          106 c          122 g          163 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cctct
//
LOCUS       DAFFSV010000327          590 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.337, whole genome shotgun sequence.
ACCESSION   DAFFSV010000327 DAFFSV010000000
VERSION     DAFFSV010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.337"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..537
                     /locus_tag="LR110_003076"
     CDS             <1..537
                     /locus_tag="LR110_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL4823967.1"
                     /translation="RWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRA
                     IDADGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGL
                     YTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGT
                     LFGFSVSTEIKVLMGITA"
BASE COUNT          199 a          111 c          125 g          155 t
ORIGIN      
        1 cgttgggtgc aagagtacag caaagtcctc tattatcttt ggaagaagaa aaatagacaa
       61 tccttctatt catggaaaat ggacgaaacc tatatcaaaa ttaagggacg ttggcattat
      121 ctttatcgtg caattgatgc ggacggctta accttagata tctggttacg aaagaaacgg
      181 gatacgcaag cagcctatgc tttcttaaaa cgactccata aacagtttgg tgagccgaaa
      241 gcaattgtga ccgataaagc accttctctt ggctccgcct ttagaaagtt acagagtgtg
      301 ggtttatata ctaagacaga gcaccgaact gtgaagtatc ttaacaattt aatagaacaa
      361 gaccatcgac ctattaaacg acgaaataaa ttttatcaaa gtctccgtac agcctcttcc
      421 acgattaagg gcatggagac cattcgagga atatataaaa agaaccgaag aaatggaacg
      481 ctcttcggct tttcggtgtc tactgaaatc aaggtattaa tgggaataac agcctaagat
      541 atttggagtt cagagagggt gcgtttgatt ttcaaacttc gcaacagaac
//
LOCUS       DAFFSV010000328          553 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.338, whole genome shotgun sequence.
ACCESSION   DAFFSV010000328 DAFFSV010000000
VERSION     DAFFSV010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.338"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(174..473)
                     /locus_tag="LR110_003077"
     CDS             complement(174..473)
                     /locus_tag="LR110_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL4823968.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          176 a          100 c           66 g          211 t
ORIGIN      
        1 tattagttta tactagctgt ttttgtgtta attaaaggat aaaatacaat ataaagaaac
       61 atctgtttct ttatttagaa taaaaaaata cagactctgt tcaaataaat gaacctagcc
      121 tgcattatga gattgactat actttttaat tcattgagat gaataactcg tttttataaa
      181 tgcccaaaag taatcggtgc cgaagccatt cctcgaccta attctttttc aaattttcct
      241 attgttgata tctccatata aaacggtgat actggtttag acaattctcc cctgattgct
      301 aacggacgta aactacgttc caaattattt atgactttag gataatactg acctttttct
      361 aagcctgtct ttgcgtctat caaaagtata cgttcctttt ctttgatctc tggatttaaa
      421 acgagatcat ataattttag taccatgttt tctatttctt cacttttaga catcacgatt
      481 tcctcccaaa aatatatttc ttcccactac attctataaa caaagtatac gctacatcta
      541 gattttttta aat
//
LOCUS       DAFFSV010000329          552 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.339, whole genome shotgun sequence.
ACCESSION   DAFFSV010000329 DAFFSV010000000
VERSION     DAFFSV010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.339"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(151..441)
                     /locus_tag="LR110_003078"
     CDS             complement(151..441)
                     /locus_tag="LR110_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL4823969.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT          186 a           80 c           84 g          202 t
ORIGIN      
        1 tgcgagcagc aactagaata atttaaacat tattaaactg tctatattgt tactaataat
       61 tcccttctta tagtgcattt tcttagaaaa aaacaatatt acgatatcat gatattaaga
      121 tatcgtaata ttatacaatt gtgattgata ttatatatgt ttttttattt ttttatcatg
      181 tttaatcttg aaggaatcat attctttttc taaagacagg agcttttcat aaacatcaga
      241 catgtttaaa ctagaggcca ggcttaaatc ataaacgaat gaagcaggta atgattttat
      301 tctacgttgt cgggttatcc agcttgcaac cgttccttgt gggtaggcat ataactcaca
      361 aaattgttct acagtgattc ctaaattctt gataatgtaa acattgatcg ggtgcgagta
      421 aacaaatgta ttgatagaca tggtgcgtaa gcctactttc ttttattatt gcgtttgcaa
      481 taatagttta acacacgcat tattctagta tctactccta tttgtaaaaa caaaaaagga
      541 ttcatagaga ta
//
LOCUS       DAFFSV010000330          552 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.340, whole genome shotgun sequence.
ACCESSION   DAFFSV010000330 DAFFSV010000000
VERSION     DAFFSV010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.340"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            116..>552
                     /locus_tag="LR110_003079"
     CDS             116..>552
                     /locus_tag="LR110_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL4823970.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAVRIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVY"
BASE COUNT          190 a          104 c          110 g          148 t
ORIGIN      
        1 tttttgtcta tataaggtat accctaacta gacacgacca caacattgaa aaatctggtt
       61 ttatcgtgta tgattagggt gtattttaaa tagttgcaga aagtaggtaa aaattttgag
      121 agttgcatac atacgtgtct catccatcga tcaaaatgaa caacgccaga ttgaagaaat
      181 gaaaaaattc ggtgcagtga gaattttcat cgagaaacaa tcgggcgcaa cgattactca
      241 tcgacctgtt ttccaagaag cattggattt cgtccgagat caagacattt ttattgtgga
      301 agctattgat cgacttggaa gaaattacga cgagatcatc gcttcagtca attacctaaa
      361 gaagaaaaac gtacagctaa tcatcaccag tctaccgatc atggctgaag caattggcaa
      421 ccctctcctg gataaattca ttaaggatct gatcatccaa attttggcca tgatcgctga
      481 acaagagcgt accgaatcta aacgacgcca ggcgcaaggc attaaaattg cgaaagcaaa
      541 tggagtttat aa
//
LOCUS       DAFFSV010000331          534 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.341, whole genome shotgun sequence.
ACCESSION   DAFFSV010000331 DAFFSV010000000
VERSION     DAFFSV010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.341"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(281..478)
                     /locus_tag="LR110_003080"
     CDS             complement(281..478)
                     /locus_tag="LR110_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL4823971.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          185 a          102 c           69 g          178 t
ORIGIN      
        1 attgtgatcc acctcttttg tttattagtt taaaccatca aaccattttt aataaaatat
       61 gtaaattcct ttgctcaaaa aagtagcttc tttacgattc gacaatttca aataaagcag
      121 gtaaaaactg gctgtcagaa aatagccaaa ctgaagcaaa aaataatgtt aaaattaatt
      181 aatgaacgaa aaaaatccct cacctaatat tacggtgagg aattttttct accgacataa
      241 acaaaaaaac cacctcatat gaggcggtta acgttcaaca ttattttacg atatttactg
      301 cttgaggacc acgttgtcct tcttcaatat cataagtaac tgcttggcct tcatctaaag
      361 acttaaagcc ttctccttgg attgctgaga aatgagcgaa tacgtcattt ccatcttcac
      421 cagtgatgaa accaaaacct ttatctgagt taaaccattt tactgtaccg ttattcatat
      481 atatttcctc ctgatacgta tataatacgt gttttattgc aattaatatt actt
//
LOCUS       DAFFSV010000332          533 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.342, whole genome shotgun sequence.
ACCESSION   DAFFSV010000332 DAFFSV010000000
VERSION     DAFFSV010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.342"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(259..522)
                     /locus_tag="LR110_003081"
     CDS             complement(259..522)
                     /locus_tag="LR110_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HBL4823972.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          210 a           93 c           74 g          156 t
ORIGIN      
        1 tttttcatgt tgaaaacatc actaatcaaa ggcaaattat aatttagaaa tagaatgaaa
       61 acactaaatg aaaatagaac attagaaaaa atagaatcaa aaaaacccaa gactagtgcc
      121 gaaataaggg aaacaatcga agctacaaaa aataatgatt tcgttgaaca ggaccaattt
      181 atttgtttcc gatatttctc aaaaagaact ttctttggga aagttaaagg cgactgtaaa
      241 gtcgccttta gtcctttttt accaagctgc tgcatattta gctccatatt ttttaattaa
      301 tgatttggct gtagcaacaa taccagctgt aattaatccg cctccaacaa ctgcagcaac
      361 aatagaaatg attgtagcaa ccgtgctacc agtattaatt atgtcaataa cttttttagc
      421 tgcataagaa gaaattccaa tgttagcact tagcattggt acaccaacaa agaatcccgc
      481 aaaacctaaa attggtaaaa ctaataataa attctttttc ataataattc ctc
//
LOCUS       DAFFSV010000333          527 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.343, whole genome shotgun sequence.
ACCESSION   DAFFSV010000333 DAFFSV010000000
VERSION     DAFFSV010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.343"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(47..349)
                     /locus_tag="LR110_003082"
     CDS             complement(47..349)
                     /locus_tag="LR110_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823973.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          180 a           92 c           74 g          181 t
ORIGIN      
        1 atggtgataa aatccgttcc gggctacctt ttttaggtag cctactttaa tctttatacc
       61 tttctggatc aacgaaagta tacttcaaat agtcataacg ccgatgatcg ctccgtgcgt
      121 ctggcacatc agtcacgata tcaaacaaaa aatacacatc tttcttcatt ctggtttttg
      181 ttgccggaat tttaaagtag ttcttattag agtaatagag attgattaat aagctatctt
      241 cgattgctaa aaagaaaact tctgaatccc acaccttata aaaatctttg acaaatctat
      301 tcgaaggatc aaatttaaac cataattgtg tttttccttc catcaacata atgttcacct
      361 caaaaagagt atacgaacta atgttctttt tgtaaagtag gaatatcatt gataatatgg
      421 atggaacata tatattaaaa agtgttcttt ttgaatgcta ttttgaatgc cattttcatt
      481 catttaacga aaatctgcga aaataaaaaa tgctcaaacc cttataa
//
LOCUS       DAFFSV010000334          514 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.344, whole genome shotgun sequence.
ACCESSION   DAFFSV010000334 DAFFSV010000000
VERSION     DAFFSV010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.344"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..132)
                     /locus_tag="LR110_003083"
     CDS             complement(<1..132)
                     /locus_tag="LR110_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL4823974.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAFFSV010000335          501 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.345, whole genome shotgun sequence.
ACCESSION   DAFFSV010000335 DAFFSV010000000
VERSION     DAFFSV010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.345"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            165..359
                     /locus_tag="LR110_003084"
     CDS             165..359
                     /locus_tag="LR110_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823975.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          215 a           62 c           78 g          146 t
ORIGIN      
        1 gaacaatagc cttttattat atatattatt caaattagtt atatcgagtg aaaaaaactt
       61 tcaaattgat tctttttatt tatcataaag atatagatac ataatcattt tcacttacat
      121 atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat tatgtagaag
      181 taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac gaatatcctt
      241 tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata aaaataaaga
      301 acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc gaaaagtaaa
      361 tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc taagattata
      421 gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt tgtataccat
      481 atgattattt caatatgtat c
//
LOCUS       DAFFSV010000336          490 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.347, whole genome shotgun sequence.
ACCESSION   DAFFSV010000336 DAFFSV010000000
VERSION     DAFFSV010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.347"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..414)
                     /locus_tag="LR110_003085"
     CDS             complement(<1..414)
                     /locus_tag="LR110_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823976.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          186 a           79 c           62 g          163 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacatac
//
LOCUS       DAFFSV010000337          486 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.348, whole genome shotgun sequence.
ACCESSION   DAFFSV010000337 DAFFSV010000000
VERSION     DAFFSV010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.348"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(26..424)
                     /gene="spxA"
                     /locus_tag="LR110_003086"
     CDS             complement(26..424)
                     /gene="spxA"
                     /locus_tag="LR110_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL4823977.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          140 a           99 c           84 g          163 t
ORIGIN      
        1 cttttccaag tttcgtatta tccttctata atccagccat caattgtgct tgacgtaatt
       61 ctaattgtcg tacatcgcgc ggtaggaacc gacgaatttc atcttcatta aaaccaactt
      121 gcagacgttt atcatcaatc atgattggac ggcgaagcaa tccaggattg ttttgcacaa
      181 gttccaataa ttcttgtaac ggcagatcat ctaaatccat gttcagtttt tggaatactt
      241 tggaacgtgt cgagatgatt tcttctgttc cgtcttctgt catttgtaaa atcgctttta
      301 attcagaact gttcaatggt tctgaaaaaa tattacgttc aacaaatgga atctgatgtt
      361 cttgcaacca cgcacgagcc tttctgcagg aagtacaact tggggaagta tataatgtta
      421 acaacgtgca ccactccttt tcgtgataag aatcactttt ttgtaacaga agatcataat
      481 aatctc
//
LOCUS       DAFFSV010000338          479 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.349, whole genome shotgun sequence.
ACCESSION   DAFFSV010000338 DAFFSV010000000
VERSION     DAFFSV010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.349"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>479
                     /locus_tag="LR110_003087"
     CDS             <1..>479
                     /locus_tag="LR110_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823978.1"
                     /translation="AISVSLIGNIALFWQLKEEQAANTYKIEEVAKNASEKLKKNSSE
                     AQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFGQLAIDKSLSRNE
                     LTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTSSVKEEQNFVVFV
                     "
BASE COUNT          196 a           83 c          102 g           98 t
ORIGIN      
        1 gcaattagcg ttagtttaat cggcaatatt gcgttgtttt ggcagctgaa agaggaacaa
       61 gcagcaaata cctataaaat tgaagaagtg gctaaaaacg cgtctgagaa gctcaaaaaa
      121 aattcctctg aggcacaggc gaagatcgac aagttaaatc aaaccatcaa tgagaaaaac
      181 caagaaatcg atcagctgaa gcaacaagat ggcacaaaag caaaagagga agtaactgaa
      241 gcacaaagaa agacggcaga agagttcggt caattggcca ttgataaatc attaagcaga
      301 aatgaattaa cggaaaaact aaaagatgtc gcaacccaag aagtgatcga caaattaagt
      361 cctacagacg atgatcacca acatgatgat tacggaagtt actcctttac attaggtgac
      421 gtgcaaactt acgctcaaac aagcagtgta aaagaggaac aaaattttgt cgtatttgt
//
LOCUS       DAFFSV010000339          473 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.350, whole genome shotgun sequence.
ACCESSION   DAFFSV010000339 DAFFSV010000000
VERSION     DAFFSV010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.350"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..371
                     /locus_tag="LR110_003088"
     CDS             <1..371
                     /locus_tag="LR110_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL4823979.1"
                     /translation="KICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRE
                     LSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPN
                     MFVNYQNDPYQFTTQVILLF"
BASE COUNT          174 a           59 c           84 g          156 t
ORIGIN      
        1 ataagatttg ttgtaaattc gaatgtccaa gtactattat tgaagttcct gttccaatat
       61 ttgactgtat tagggtgctt ggaattattc tcgataatgc aattgaagca tctatagaga
      121 gtcaagatag agaactgtct ctagctattt accgagatga taaacagata gaaatttcga
      181 tatcgaatag ttgtagagag atactactct ctatagatac tctaatgaaa aaaggaataa
      241 caacaaaaaa gggacatcaa gggttaggtt tatctagtat agaggaaatc aataaaaaga
      301 atcccaatat gtttgtgaac tatcaaaacg atccgtatca atttactact caagttattt
      361 tgttgttttg agggaaggag gatttatgag ttatccaata atattatgtg aagatcaaat
      421 tatacaatta aatcagttag aacgaattat tgataatttc attctttttc acg
//
LOCUS       DAFFSV010000340          466 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.351, whole genome shotgun sequence.
ACCESSION   DAFFSV010000340 DAFFSV010000000
VERSION     DAFFSV010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.351"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>466
                     /locus_tag="LR110_003089"
     CDS             <1..>466
                     /locus_tag="LR110_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL4823980.1"
                     /translation="SLTKELSNPSLKREIEYSQSEDLLKALKWSEEVNQQISQAKGLD
                     KPFLSARDTIKALKKYGKIIIVSSANKEAVQEEWERHELLSLVDELCCQDKGKKEDII
                     RSVIENGCDLDKILMIGDSPGDLEAANKNKVFFYPILVNKEKESWENLRTDVL"
BASE COUNT          186 a           47 c           92 g          141 t
ORIGIN      
        1 tctctaacaa aagaattgtc taatccatct ttgaagaggg aaatagaata ttctcagtca
       61 gaggatttat taaaagcttt aaaatggtcg gaagaagtca atcaacagat tagtcaagca
      121 aaaggattag ataaaccctt tttaagtgct agagatacta taaaagctct gaaaaaatat
      181 gggaagataa ttattgtaag ctcagctaat aaggaagctg ttcaagaaga atgggaaaga
      241 catgaattat tatcattagt tgatgaactg tgttgtcaag ataaaggaaa aaaagaagat
      301 attattcgaa gtgtaattga aaatggttgt gatttggata agatattaat gattggtgat
      361 tctcctggtg atctagaagc tgcaaataaa aataaagttt tcttttatcc tattttggtc
      421 aataaagaaa aagaatcatg ggaaaactta agaactgatg ttttat
//
LOCUS       DAFFSV010000341          462 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.352, whole genome shotgun sequence.
ACCESSION   DAFFSV010000341 DAFFSV010000000
VERSION     DAFFSV010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.352"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..430)
                     /locus_tag="LR110_003090"
     CDS             complement(<1..430)
                     /locus_tag="LR110_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL4823981.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAFFSV010000342          455 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.353, whole genome shotgun sequence.
ACCESSION   DAFFSV010000342 DAFFSV010000000
VERSION     DAFFSV010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.353"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            133..>455
                     /locus_tag="LR110_003091"
     CDS             133..>455
                     /locus_tag="LR110_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL4823982.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGR"
BASE COUNT          172 a           65 c           87 g          131 t
ORIGIN      
        1 aattagaggg aacgcatgtt tgaatgcgtt ccctcttttt tgtttgtcat gaacgaaaat
       61 caattctcta tttttgaaaa ataacaagaa aaacttgtta aaaatattta aagtaagtgg
      121 gtggattatt ttatgtcata tacaggaatt ttgagtctcg aagatatttg tcactacggt
      181 aaacggtgta ccgctacaga aaagatcacg aagaaactat caactggtca aagtaaaacc
      241 gttgtgcagt gtaagaaata tataatccag aaagacaaag tctcagaaga aatgatttat
      301 tacattggga aacaaaaaca aataatttta aaagatccta tccctttaaa agagctatat
      361 ccaacaatca aacatgttta tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga
      421 gtcttgcgtt gtaccgcaaa agggatgggg cgttt
//
LOCUS       DAFFSV010000343          450 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.354, whole genome shotgun sequence.
ACCESSION   DAFFSV010000343 DAFFSV010000000
VERSION     DAFFSV010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.354"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..349)
                     /locus_tag="LR110_003092"
     CDS             complement(<1..349)
                     /locus_tag="LR110_003092"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBL4823983.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVER"
     gene            complement(359..>450)
                     /locus_tag="LR110_003093"
     CDS             complement(359..>450)
                     /locus_tag="LR110_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBL4823984.1"
                     /translation="NYIEKSVLVAIAVFIGKTRLIDNFTFELQ"
BASE COUNT          137 a           97 c           32 g          184 t
ORIGIN      
        1 ctctttcaac tttattatta tcatcgacaa aaacaacctt aggtctatga tccttaacct
       61 cattttcatt taattcacaa tatgccatta aaattatttt atctccagtt gatacacatc
      121 tagctgcagc tccattcaag caaatcatcc ctgttcctct ctctccagct attgtataag
      181 tttcaaatct atttccatta ttaacatcaa ctatttgtac tttttcatat tcatatattc
      241 ctgctgcttc taataaattc atatcaacag taatacttcc tacataatct aactcagctt
      301 gttcaactgt agctctatgt atttttcctt ttaacattgt aactttcatt atagccccct
      361 attgtaattc aaatgtaaaa ttatctatta atcttgtttt tcctataaat actgctattg
      421 caactaaaac tgatttttca atataattta
//
LOCUS       DAFFSV010000344          437 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.355, whole genome shotgun sequence.
ACCESSION   DAFFSV010000344 DAFFSV010000000
VERSION     DAFFSV010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.355"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          154 a           65 c           63 g          155 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaagctaaa actcctatta atcgatctac taaaaataaa
       61 taaaaaaatt cttttcaatt ttatttatgg tatgacataa ataaagtaaa gttattcttc
      121 taggttaatc ttattaatac tctttctcac tagtgttgca tgcaacacta gtgagaaaga
      181 gtattaaata tactgcaagg caagacattt ggtaattgaa gaataacaag caaaaaaata
      241 gcagtcaggt ctcttgacaa agtatagttg ctattctttt tttaggccct actttaatga
      301 aatttttgtt tggagggaac gacttcattc ctttgattat ttcagttgaa tttaatagag
      361 aagcctggcg tctattaaga aatacttttc ttgatgagcg tcaatttttt taacggaaat
      421 ctatcctata ctaaatt
//
LOCUS       DAFFSV010000345          430 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.357, whole genome shotgun sequence.
ACCESSION   DAFFSV010000345 DAFFSV010000000
VERSION     DAFFSV010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.357"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>430
                     /locus_tag="LR110_003094"
     CDS             <1..>430
                     /locus_tag="LR110_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBL4823985.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DAFFSV010000346          429 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.358, whole genome shotgun sequence.
ACCESSION   DAFFSV010000346 DAFFSV010000000
VERSION     DAFFSV010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.358"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(78..150)
                     /locus_tag="LR110_003095"
     tRNA            complement(78..150)
                     /locus_tag="LR110_003095"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:His,seq:gtg)
     gene            complement(212..296)
                     /locus_tag="LR110_003096"
     tRNA            complement(212..296)
                     /locus_tag="LR110_003096"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Leu,seq:caa)
     gene            complement(317..387)
                     /locus_tag="LR110_003097"
     tRNA            complement(317..387)
                     /locus_tag="LR110_003097"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(353..355),aa:Cys,seq:gca)
BASE COUNT          126 a          106 c           95 g          102 t
ORIGIN      
        1 ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct
       61 ataccccaag aataaagggc gaatgatggg aatcgaaccc acgaatgccg gagccacaat
      121 ccggtgcgtt aaccacttcg ccacattcgc catcataaaa gatgttattt aatttctcaa
      181 taaaaaacta agatgaagac atcttagtca ataccggcgg ccggggtcga accggcacgc
      241 cctcaacggg cactggattt tgagtccagc gcgtctgcca attccgccac gccggcaagt
      301 tgatttatta gtactaaggc ggtaaccgga tttgaaccgg tgatgaaggt tttgcagacc
      361 tctgccttac cacttggcta taccgccatt atactattat caatctataa aataaagaat
      421 aaattatag
//
LOCUS       DAFFSV010000347          416 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.359, whole genome shotgun sequence.
ACCESSION   DAFFSV010000347 DAFFSV010000000
VERSION     DAFFSV010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.359"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          170 a           59 c           72 g          115 t
ORIGIN      
        1 cattattatc aaaataatgt taaaaataaa aaagaattgt ccatcactgt tattcaaatc
       61 attttatcaa aactacatag taaatgttaa aaagcaacat gacataatat atagaaggct
      121 ttacagtata tatgggagct tactaaaaaa gaatggagta agagttaaca caatgggcca
      181 ttagctgaag ttttaagcac ttcaaggtat gaaacagaat ggcctgtaat actacatgat
      241 tatttagact gctcaaactg ctatcagtct ggtggaatga ataaagtatt atactaaaag
      301 cagatccaac tacaacgtaa caggaaacca gttgtatcag gaaaagtgat agtacatgga
      361 gaagaaaagg ccccctttgt atgagtcagt acatatctta tttatagata aaacgg
//
LOCUS       DAFFSV010000348          405 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.360, whole genome shotgun sequence.
ACCESSION   DAFFSV010000348 DAFFSV010000000
VERSION     DAFFSV010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.360"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..374
                     /locus_tag="LR110_003098"
     CDS             <1..374
                     /locus_tag="LR110_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4823986.1"
                     /translation="SSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSF
                     ARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFF
                     LYPLSKDKVLENCELLKQKHK"
BASE COUNT          130 a           46 c           84 g          145 t
ORIGIN      
        1 ttagttcggt tggctatatg ggtttgggtg tgtttaatac tgttatttgg gcaaatatta
       61 ctgatgttat tgatgatcaa gaagtaaagt ctgatcaaag agaagatggg accgtatatg
      121 cggtatattc ttttgctaga aaaataggtc aagctctagc tggaggcgtc ggaggttgga
      181 ctctttctat tataggctat gactctttag caaatgtaca aacgggagat gttttagaaa
      241 agttatataa cgcttcaaca ttaattccat caatttgttt tctgattgta ggattaattt
      301 tatttttcct ttatccatta tctaaagata aagtcttaga aaattgcgag cttttaaaac
      361 aaaaacataa ataggagaga aatatgaata attggatatt ttgta
//
LOCUS       DAFFSV010000349          400 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.362, whole genome shotgun sequence.
ACCESSION   DAFFSV010000349 DAFFSV010000000
VERSION     DAFFSV010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.362"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..82
                     /locus_tag="LR110_003099"
     CDS             <1..82
                     /locus_tag="LR110_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBL4823987.1"
                     /translation="ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            83..>400
                     /locus_tag="LR110_003100"
     CDS             83..>400
                     /locus_tag="LR110_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBL4823988.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFD"
BASE COUNT          156 a           49 c           81 g          114 t
ORIGIN      
        1 tgaaaacgta gtaacaggca aagaaaactg gaatgattta tatcatgcaa acgaagtatt
       61 gaaaatagct gaggagaaat aaatggaaat tttatttact atttgtggta gagctgggtc
      121 taaggggttt aaaaataaaa atttgaaata tttggcagga aagcccctag tccatttcgc
      181 tttagcaatg atagatatct atcaacaaaa acatcctgaa cataattgca cgacttgttt
      241 gaatacggac agtaatgagt tagtagaatt gacgactaag ttaaatccgt ctgtgaaaat
      301 tgtagaacgt aaacctgaat tagctacaga tactgtaggg aaaattgaag tgatacgtga
      361 ttcattctta caactagaaa gagaagataa tatatttgat
//
LOCUS       DAFFSV010000350          398 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.363, whole genome shotgun sequence.
ACCESSION   DAFFSV010000350 DAFFSV010000000
VERSION     DAFFSV010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.363"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          145 a           51 c           58 g          144 t
ORIGIN      
        1 gtactttatt atcaagccat tattccttct tctactagtt ctttttgtca cataattttg
       61 caatcaataa atgttgcgca ataagtataa gaattccatt ttctaaattt gaaaggtgat
      121 ccataatttc atgttcaagt agtttattaa ttgtccactt gtcgcttcat ctaaaaattt
      181 tataggtttt aggaatgcta ttcgcttatt ttaggaagat tttaactgtt tgctatagat
      241 atatatcata ctagtatcgt aataaaagta ctctaaaaga agaattttca aatacgcaaa
      301 attaagtacg aaagagttga aacaaatttg gagaaaatga gcatagaatg tctgatgagt
      361 taaatagacc taaaaattat ctaaaagtgg agcaaaat
//
LOCUS       DAFFSV010000351          384 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.364, whole genome shotgun sequence.
ACCESSION   DAFFSV010000351 DAFFSV010000000
VERSION     DAFFSV010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.364"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>384
                     /locus_tag="LR110_003101"
     CDS             <1..>384
                     /locus_tag="LR110_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823989.1"
                     /translation="FKKRNSGKVFNSNGNVYYNYFYFVFWVAATLLGYDKIQIAGIPI
                     HMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSIEKENFDDIDGYDSVNLLIIDTYDI
                     KMSELSMENQTYPTIIVRGNSIDGVR"
BASE COUNT          136 a           43 c           76 g          129 t
ORIGIN      
        1 ttttaaaaaa agaaattcag gaaaggtgtt taactctaat ggaaatgtgt actacaacta
       61 tttttatttt gtattttggg tagcagcaac tttacttggt tatgataaaa ttcagattgc
      121 aggaattcct atacacatgc aatataagtt ggttttgagt ggaatttttt ctgaggtgtt
      181 gccagatata tacgatgatc attacgacag tgatggaaca tgtaaagtaa gcattgaaaa
      241 agaaaatttc gatgacatcg atggatatga ctctgtgaac ttactgatta ttgatactta
      301 cgatataaaa atgagtgaat tatctatgga aaatcaaaca tatcctacga taattgttag
      361 agggaattca atagatggag ttcg
//
LOCUS       DAFFSV010000352          379 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.365, whole genome shotgun sequence.
ACCESSION   DAFFSV010000352 DAFFSV010000000
VERSION     DAFFSV010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.365"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..375)
                     /locus_tag="LR110_003102"
     CDS             complement(<1..375)
                     /locus_tag="LR110_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL4823990.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIK"
BASE COUNT          101 a           63 c           65 g          150 t
ORIGIN      
        1 ttttattata ggattcaata actcatagtc tttatctagc gaaaacgtat cactatatct
       61 ttcttgaatg tctttaaaga ctttgtgagg tatttctggg aagtccttaa tatatagctc
      121 ataccctata tcgataaggt atgaagttat atattcttct aaagttcccc atttaatcca
      181 gtcaattgtt gaattagtga tagttgataa taatttcagc ttttgtggat ttggtaaatt
      241 ggtcccacgt tcccagttgg atatggtcgc acgtggtgaa ttatctattt tccttccaag
      301 ttcttccata gtaaaaccac atgaaaggcg taattgtttg attcgttttc ctgcttctga
      361 aggatttggc ttcatcttt
//
LOCUS       DAFFSV010000353          376 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.366, whole genome shotgun sequence.
ACCESSION   DAFFSV010000353 DAFFSV010000000
VERSION     DAFFSV010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.366"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>376
                     /locus_tag="LR110_003103"
     CDS             <1..>376
                     /locus_tag="LR110_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HBL4823991.1"
                     /translation="LSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQ
                     AKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEA
                     NLPNILGDKGYISHKIHEKLAL"
BASE COUNT          135 a           70 c           60 g          111 t
ORIGIN      
        1 gcttaagttc aaacttagct tacactatca agattattcg atactttttc atcaagaagc
       61 tcaccaaagg tgaactagtc ggaattatag atagttttcc tagtccatta tgtaaaccag
      121 ttagaaatag acaagcaaaa ctattgaatc aaatagccaa agttggctat aactcaacaa
      181 aaaaatctta tttttatggt cttaaaattc atatgatcgt tactaagact ggctttccga
      241 ttacttactc aatcacgaat ccaggtgtcc atgacgtgaa agtgttagag actttatcgg
      301 aagaagcgaa tcttcccaat atcctagggg acaaaggata tattagccat aaaatccatg
      361 aaaagcttgc ccttaa
//
LOCUS       DAFFSV010000354          359 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.369, whole genome shotgun sequence.
ACCESSION   DAFFSV010000354 DAFFSV010000000
VERSION     DAFFSV010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.369"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          140 a           42 c           73 g          104 t
ORIGIN      
        1 aacaattttg tggagcgtga agcttttatg gtagaggaaa atatgaataa agtgatgttg
       61 ataaaaagat tcctatcttg aatatgaatc gagatgtctt gaaaatgaat gagtttgacc
      121 aatcattatc ctatcgaaat ttacagtttc gttcgattgg tctgtcaatt aatgaaaaag
      181 taaaaaagaa tatgttctag gaagagggcg gcacatctaa atttttaagt aacggacagt
      241 tcaaatatca agcattgaaa gaggctatcc tactgtttga aggaatacga atatataaaa
      301 acgacaaaga ggaaaaagta gttcattaaa tcccttcaag tgatgaaaga atgagatct
//
LOCUS       DAFFSV010000355          354 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.370, whole genome shotgun sequence.
ACCESSION   DAFFSV010000355 DAFFSV010000000
VERSION     DAFFSV010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.370"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..>354)
                     /locus_tag="LR110_003104"
     CDS             complement(<1..>354)
                     /locus_tag="LR110_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823992.1"
                     /translation="DTLEAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFL
                     KAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTT
                     VAMFAEKYTYEETTIL"
BASE COUNT           88 a           77 c           68 g          121 t
ORIGIN      
        1 caagatcgtt gtttcttcat aggtatactt ttccgcaaac atggccaccg ttgtttttaa
       61 cacttgttca taaatagcct gctgcagtgg acttagttgt tcaaaagccg ctgctgtcat
      121 aaccgttttt gtaggaatga tcgcgtaatg ttcctggact ttcttattat tcacataccg
      181 actattcggt gtatgaatag gagtcggaat cgcttcggct tttaaaaaat gcttgtattc
      241 gtctaagtga tccaacagat acgcatattc cccttccgta atataagggg tatccgttcg
      301 cggatagctt agatattttc cttcatacaa cccttgcatg gcttccaaag tatc
//
LOCUS       DAFFSV010000356          354 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.371, whole genome shotgun sequence.
ACCESSION   DAFFSV010000356 DAFFSV010000000
VERSION     DAFFSV010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.371"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            15..>354
                     /locus_tag="LR110_003105"
     CDS             15..>354
                     /locus_tag="LR110_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL4823993.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTHQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVI"
BASE COUNT          142 a           51 c           75 g           86 t
ORIGIN      
        1 ttaggaggaa gaaaatgagt aagaaaaaag gagaaagaca acgtttcaaa aaacgaaaaa
       61 gttatcttca atggctggga atctttatga ttctcggctt tttgattctt gctagtccga
      121 tttacacgca tcagaaggta gacataccag atgaagtaag cacgaacgca tttttaaaca
      181 cgaaggaact aacgatggtt tcaaaagaaa aaggagcgaa ccagcaatta gtttttgaat
      241 ttgcaatcga cgatgaagat caaagcttat taaaagaact ggctaatctt aattatcgcg
      301 tagaagtaaa gacagctaaa ggggattatc aagcaataaa agccaaagtg atta
//
LOCUS       DAFFSV010000357          352 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.372, whole genome shotgun sequence.
ACCESSION   DAFFSV010000357 DAFFSV010000000
VERSION     DAFFSV010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.372"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          100 a           56 c           80 g          116 t
ORIGIN      
        1 agttggtcag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacagggtac
       61 ataatttaat ctatttgata aaaaataaaa ttcaataatg gtataataaa atatcggctc
      121 gtcgcttcca cagagatttt cgtgggagga ggtgtatgat ttgtttgaat tcactttgga
      181 gcacgtgctc ttgccattgt taattggttt aactttggca ttatttagtc attggctaga
      241 taaacattag agccgacaat ggcgcttact gtttcgcagc ggtaagcgtc tttttttgaa
      301 aaagaaaaaa acacaacgta ttcgcaggta cgttgtgttt atggtgtata tt
//
LOCUS       DAFFSV010000358          350 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.denovo.373, whole genome shotgun sequence.
ACCESSION   DAFFSV010000358 DAFFSV010000000
VERSION     DAFFSV010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.denovo.373"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..>350)
                     /locus_tag="LR110_003106"
     CDS             complement(<1..>350)
                     /locus_tag="LR110_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL4823994.1"
                     /translation="TSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQT
                     NIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGF
                     SIFNYNFYAAGLNL"
BASE COUNT          122 a           55 c           56 g          117 t
ORIGIN      
        1 ttaaattcaa acctgctgcg taaaaattgt aattaaatat agaaaagcca tttgtaaatt
       61 gggaaccaga aggtgtaaaa tcttgattgt caatatgttg agttgcaata taagctgttc
      121 ttagagcatt agaaatagaa aaatcaacac tacttctatt aatggacgta aaaaaagttg
      181 tagcacaatc cccaatagca tatatatctt ttatatttgt ttgctggtat tcattagtta
      241 gatatgctcc actaacatct ctcaagagtt cctttttgcc aattggacta ttagatagaa
      301 atcctatagc ttgtacgacc atatccatag agtattgggt accacttgta
//
LOCUS       DAFFSV010000359         5814 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.guided.142, whole genome shotgun sequence.
ACCESSION   DAFFSV010000359 DAFFSV010000000
VERSION     DAFFSV010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.guided.142"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            333..797
                     /locus_tag="LR110_003107"
     CDS             333..797
                     /locus_tag="LR110_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL4823995.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            781..1410
                     /locus_tag="LR110_003108"
     CDS             781..1410
                     /locus_tag="LR110_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL4823996.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1764..3242
                     /gene="msr(C)"
                     /locus_tag="LR110_003109"
     CDS             1764..3242
                     /gene="msr(C)"
                     /locus_tag="LR110_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL4823997.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3289..3735
                     /locus_tag="LR110_003110"
     CDS             3289..3735
                     /locus_tag="LR110_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL4823998.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3934..4449)
                     /locus_tag="LR110_003111"
     CDS             complement(3934..4449)
                     /locus_tag="LR110_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL4823999.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4566..5768
                     /locus_tag="LR110_003112"
     CDS             4566..5768
                     /locus_tag="LR110_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL4824000.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1799 a          968 c         1230 g         1817 t
ORIGIN      
        1 acttttttat tactgtttaa gcatactgat ccaatttcta atattcgacg ggattcttgc
       61 aaaaggcgat ttcggaaatt tttggcggtg taaaattatc tactgcagaa gagctatctt
      121 ctatagtttg ataagaatca ataatcttgg ttttagcgtt gtgctttctt tatcattttt
      181 tccgttcatt ctacaaaaaa tttcttttct ctatactttt ctattgagag cgtttttttt
      241 attacacagc tgattctttg acttttttct ttttttattc tattattgac aagtcaatcg
      301 ttgacgagtc aattattaga aaggaggaaa ttatgtcttt agaagagatt agtcaccttt
      361 tatatcaaat caaattagcc gatcaaaaaa tcgtccagat ctttgaagaa aatatcggta
      421 taagtcttac tagatacgag atcatgatga gactgaaaga agaaaatcca ttgctacaaa
      481 gcagtttgca agaaaagttg cagattgatc aaggagcagt tactcgtcat ctgaaggtat
      541 tggaagcaaa tggctatgtt tcgcgtcaga gaaatcctca gaacaaccgg gaagtgtatg
      601 tagcgctaac agaaaaagcc cagaagaaaa tcaagcattg tgaaatggac cataaagata
      661 caggttccgt gctatccccg gctttcagcg atgtagaaat gaaacaattt ctaacacttt
      721 tagataaatt gaatacccaa ctttgcaata taacaacaga aacgaaagag gttaataata
      781 atggaacaaa aaaatgattt ttcaacaatt atgactggta gaagatccgt tcgcgtatat
      841 gatgaaacat acaaaatccc acacgaagaa atgctggaaa tgatcaaaga agcgacgact
      901 gcaccttctt ctgtcaatat gaaaccatgg cgctttgtgg tcgctgaaag cgaggaagca
      961 aaagaaatct tgcgtccttt gatccgtttc aatacgagac aaaatgatac ttcttctgca
     1021 atggtcatga tttttggcga tatgcaatgt tacgaatatg gcgaagaaat ctacaaccaa
     1081 gcttacgaat cagggaagat gccaaaagaa gtcagagatc aacaattagc tgcgatcatt
     1141 ccttattaca agagtttttc aagagaagaa atgaacgatg tagtcaaagt cgattctagc
     1201 ttagcggcta tgcaattcat gttagttgcg cgtgatcatg gctatgaaac aaatccaatt
     1261 ggtggattcg aagcagatca gttggcagag gcatttggtt tggataaaga ccgttatgtt
     1321 ccagttatta ttttatcaat aggaaaagcg gtagaagaag gttacgagtc tgttcgttta
     1381 gcaccagaaa agatcactat gttcgaataa aaggttttca gttactattc agtagctatt
     1441 gctttttttg aatcgtgtgt ggtaacctaa taaaaaagga ggtcggaaaa atgactgcat
     1501 cgatgaaatt acgtttcgaa cttttgaata acaactaaat aaattcaaaa gacatgtata
     1561 aacaacaatt tcatggatgg agtatgtcca ttttttcgaa cgacagtaga ataggtgaaa
     1621 gctatgacag atttggtgat ttagtcatag ttatttcgtt cagagtagga aaagatgtga
     1681 tgcagttttt ttgcaaacag atttttctgc tcttttttag tatcgataat tgttgtttta
     1741 catgaaatag gagtgtaaaa catatggaaa atttagcagt aaatataaca aatctacaag
     1801 tcagctttgg caaccagcta gaactatcta ttgattctct tcgtgtctat cagcaagatc
     1861 gtatagggat cataggagaa aatggtgtcg gtaaatcgac tttgctcaaa ctaatagccg
     1921 gtgaactttt tcccgatcat gggaaaatcc aaacagagat caccttcaac tacctgcctc
     1981 aattaaccta tctcgctgaa gcaaaggacc taaatttgga attagccagt catttccagt
     2041 taagactgga agaaacttca gagcggaaat ggagcggagg ggaagaacga aagatcgagt
     2101 tgatacgtct tctttcttct tatgaacaag ggatgcttct agacgagcca acaacccatc
     2161 tagatagaaa aagtattgat cgactgattg aagagcttcg ttattattat ggtacgctgg
     2221 tttttgttag tcatgatcgc tattttctag atgagctagc atcgaaaatc tgggaagtaa
     2281 aagacggaga aatccgagag ttttcgggga attatagtgc ctatctcact caaaaggaat
     2341 tggagaaaaa gactcagcta cgagaagcag agtcgatcat gaaagagaaa aaacgattgg
     2401 aaaaatcgat ccaagaaaag aaaaaacaag cggaaaagtt ggaaaaagtg tccagtaaaa
     2461 agaaaaagca acaaatcaga ctggatcggt tgtcttcctc taaacaaaaa gacagtgtac
     2521 aaaaagccat ccaaaagaat gcgaaaacat tagagagaag actccaaaaa ataggagaaa
     2581 caaccaagcc gcagcagatg aaacaaatcc gttttccagt accaaaatct cttgaactcc
     2641 acagccgcta tcccatcatg ggacaaaatg tccaattgga aagaagtggg agaacattac
     2701 tggtaaatgg tgattttcag ttttctttag gtaaaaaaat cgcgattgtc ggtgaaaatg
     2761 gttcaggtaa gacaacctta ttagaacata tccgcaaaca aggagaagga atccttctct
     2821 ctccgaaagt aagctttcaa gtatatcagc aaaagggtta tcaaatgaca tctgaagaat
     2881 ccatcattcg ttttgtcatg agacaaacag agttttcaga atcgcttgtc cgtagtttgc
     2941 tgaatcactt agggtttgct caggaaactt tgacgaaacc gttatgtaca ttaagtgggg
     3001 gagaagcgac ccgtctgacg attgctttgc tttttactaa gccaagtaat gtgttgctgt
     3061 tagatgaacc gactaatttt attgatatgg caacgatcga agctttagag aagctaatgc
     3121 aaatatatcc gggaacgatt ttgtttactt ctcatgattc ttactttgtc gagcgtacgg
     3181 ctgatgaagt ttatgaaata aaagggcaga aaataaaaaa agtacttacg agaaattttt
     3241 aaatgtgcta cgatgatttt cgtgacaaat ttaaaggagt ggtacagatt gcctacaaat
     3301 aaaaaagaag gaataatttt tacaacaatg atgtgttttt tgatggtctt ggggatgagc
     3361 gcgtataatt tatggctgca tcaagatctt catttatcaa gtttgttgat tggtctagtg
     3421 cctggattcg tggtggcttt cattttggat gtcttcatca tcggtgtgct ggcgaaaaaa
     3481 attgccttta aactaccagt aaatccagaa agcaaggtcc aaatgatctt aactatttca
     3541 tgcttgatgg ttttaggaat ggtgacatgt atgtctttat tcggtgtgat catggaacaa
     3601 ggaatacctg atcaattatt ctctgcttat atgaatgctt ggaaaatgaa tgtgattgcc
     3661 gctttaccgt tgcagttgat catcgttggc ccgctttcaa gaagtgtgct aaaagcaatt
     3721 caaagtaatg aatgatggaa cactgttatt tgaaaaaagg ccttggcatg ctttttgtca
     3781 tagtctcttg aatgcagttc tagaaaaata gtgatccgaa cgataggttt tggcctgtcg
     3841 ttcggatcgc tttttctttt ccaactgtat tgctctttta ttgaagataa tttgctcttc
     3901 aatagatatt tctagaaagg ttctgtttat cccttactca aaaaactgct gcatttgttc
     3961 tcttacttgc tgacaccatt ggagttcctg ctgacaaagc ataattctgc gctggatcac
     4021 aacataacgt ccaaattctt gtttcttgtc tagcgagagg tcattcaatt ttttttgatt
     4081 tttttgcatg attttgttca gctgttgttc tctagtgaat agcagtccag tagctgttgt
     4141 tttatcgagc gtcgaaaaag catagatttt tgctagaaat tcatcttttt gcgtaggagc
     4201 gggagtttcc tctttgatcc attcctctac aagagcacgt ccttcatcag tcaagtggta
     4261 gatctttttt tgagagtgct cgtctttgtc caaaacggtc acataaccct gctggtctaa
     4321 cttggcaagc gttgtataga tctgactgtg atgggcttcc caaaaaattg tgatcagctg
     4381 cttcaattca tacccagaac gtggtttgga taaaagcacg cataataata tataggctaa
     4441 agtattcata aaaatcctac ctctcgtctt attttcttta ttatatcaca gtaaagtttc
     4501 cacgttttac atgtgcatat tgacatgtaa aataaatcat gtatgatgct aatagagaga
     4561 aagggatgga aatattggag aatcaaatag atatacaaga acaaacaaat gaagaaacaa
     4621 aggggaaaag ttcagcgcta gttgtagcag ctacctgtat gatcgctttt atgggagtgg
     4681 gtctagtaga cccaatctta aaaacgattg ctgcacagtt aaacgctacg ccagctgaaa
     4741 cgaccctgtt atttactagt tacatgttag tcactggtgt ggtgatgcta tttacaggtt
     4801 tcttatctag tcggatcggt ttgaaaaaaa cattgatgac aggattgttt atcatcgttg
     4861 tattcgctgg tcttggcgga ttatctggga atatcggaat gctgattggg ttacgtgccg
     4921 gctggggtgt cgggaatgct ttatttgtct ctactgcttt agcaacgatc gtcagtattt
     4981 tagttggtaa cacagaaaaa gcaatcatga tgtatgaagc agcacttggc tgcggaatgg
     5041 ctgttggccc tctagtcggt ggggtcttgg gcagtggttc atggcgttat ccattttttg
     5101 gggtagcatt tttgatgttt tgcggtttta tagccctagc ggtactttta cctgaaactg
     5161 agaaaccaaa gagaaaagta ggcctttggg aaggtgtcaa agcgttaagc aatcataaac
     5221 ttcgttcagt tggactaatc gcattgttat ataatttcgg attctttact ttacttgcgt
     5281 atgcgccatt tttattgatt ggctattctg aattagaagt cggttttgta ttttttggct
     5341 ggggtgtgct tctggctatt gcttcgattt tcgttgcacc ggctcttgag cggaagttta
     5401 cgacttatcg gacaatttta gcagcatttg tcttattcat tatttgtttg ctgttattag
     5461 gagctggaac ttctatgcca gtccttgtac cgatcagcat tgtgttagca ggatttttcc
     5521 aaggaattat caatacactg ttgacgacga ttgctatgga aataccaggt ctgcaacgaa
     5581 acgttgcttc gtccagctat agttttgtcc gctttttcgg tggagcttta gcaccattta
     5641 ttgcaggtgt gattggtgaa catattggtg aaagctatgc gttttatttt gcaggtgcca
     5701 ttgttcttat cagtgtatgt tgtatcattt atcaccgtaa ttattttgct acaaatgaag
     5761 aagaataaga aatcagaggt tgtgaaaaag ctgtcctgca tcaagtcata agaa
//
LOCUS       DAFFSV010000360         3678 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.guided.178, whole genome shotgun sequence.
ACCESSION   DAFFSV010000360 DAFFSV010000000
VERSION     DAFFSV010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.guided.178"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            88..1209
                     /locus_tag="LR110_003113"
     CDS             88..1209
                     /locus_tag="LR110_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL4824001.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1467..2969
                     /gene="eat(A)"
                     /locus_tag="LR110_003114"
     CDS             1467..2969
                     /gene="eat(A)"
                     /locus_tag="LR110_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL4824002.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2997..3563
                     /locus_tag="LR110_003115"
     CDS             2997..3563
                     /locus_tag="LR110_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL4824003.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1259 a          627 c          819 g          973 t
ORIGIN      
        1 atatagaaaa atggaagaaa atagcattgt tttgtttgaa aaaaatgaaa aaaagtgtct
       61 tacttaagaa gaaagtgagg gatacagatg gatattttgg cggcaattga ttcatttaaa
      121 ggaagtgctt cttcatttga actaaatcag gcagttctaa atggctggga agatcgtaaa
      181 ggaagaaaaa tcaatgtgcc gattgcagat ggcggagaaa atactgcaga agcaatctat
      241 tacgcgctca ggggaacatg gcgtaccatt gatgggatgg atctcttatt ccgtcagcga
      301 aaaatcagct atttattgac aacttggcaa ggaaaaaaca ccgcagtcat cgaatctgca
      361 caagcattgg ggttagatct acttgataaa ccaacggatc aaactatccg acttgcttcc
      421 agttatgcct taggagaact tattaaagat gcactgcttc aagaagtaga acaaattatt
      481 gtcactttag gaggaagcgg gtgtagcgat ggtggtctag gattactcca atccttgggt
      541 gcttctttag aagggtacac agaaggaaat cctttattat ctactaaaaa acttcatttt
      601 gatcgaagaa acgttcttgt ttctaaagta aaattgatta tcgctgcaga tgttaccaat
      661 ccatatacag gtcaacaagg agctgcttgg caatttggaa gacaaaaagg aggaatagat
      721 acgacgctag ctttcttaga taaacaagca ggaaagattg ctcagcaaat aaaagatttc
      781 tgggaggtgg atttagatga tattccagga agtggagcag caggcggtat tggtggagcc
      841 ctttttttgt tgggagcgaa gatggtttct ggtttcgatt tagtcagcca aatcactgga
      901 ttggaaaaaa agatcgaaca agcagatgtc atcattacag gagaagggcg gatcgatcag
      961 caaacgatcc acggaaaagt tccatacggt gtagcagtgc ttgcaaaaaa atatcgaaaa
     1021 ccaataatcg ctatttgcgg aagtagagac caagagctag aaggactttc tgattttctc
     1081 ccaattgtct taagtattca tttgggtcca ataggtttag aagaagcaat ggaacacagg
     1141 caaacattag aaaaaacaaa aatcttagga cagacactta gcagattttt caccatcctc
     1201 ccgaagtagt tgacaaaatc attttcttta tgctatcgta atttcaaata taggacaaag
     1261 tgagggatcc gtgcattgaa aaattaagtc gattgatgaa agctgcattc agactgtacc
     1321 ttgtggagac aaggaagcag tgtgcagatt ttcgggattg gcttggtttt ttcgatgtat
     1381 gggtcttttt gtgtgtatcc aacccttgca tcctctttag ccagttctga aaataggact
     1441 ggtctttttt tacgaggtga tggaaaatgt ctaaaatcga aataaaaaat ctgacattcg
     1501 gctacgacag ccaaggcaca ttattatttg aacaagcaaa tctaaatttt gacacacaat
     1561 ggaaactagg acttatcgga cgaaacggtc gaggaaagac aactttactg aatattctac
     1621 aaaacaaact accttatcaa gggcaagtaa tccatcagca agaatttgcc tatttcccgc
     1681 aacagacaaa agataaagaa cgtttaacct attacgtgtt aaatgatatt acggattttg
     1741 agatatggga aatcgaaaga gagctccaat tgatgcaaac agatcctgaa atcttatgga
     1801 gagaattcag cacactatcg gggggagaga agacaaaagt cctactggca cttttatttg
     1861 tggatgacac tcatttcccg ttaatcgatg aaccaacgaa tcatttggat atctctggta
     1921 gaaaacaagt agcggcttat ttgaaaaaga aaaaacaagg cttcatcgtg gtcagccatg
     1981 accggggatt tatcgatgaa gtagtggacc atgttttagc aatcgaaaaa agtcaactgg
     2041 aactttatca agggaatttc tctatctatg aagaacagaa aaaacttcgt gatgaatttg
     2101 aaatggctca aaatgaaaaa ttgaaaaaag aagtcagtag gctaaagaaa acagcagctg
     2161 aaaaagccga atggtctcgt tcccgagaag gagataaaac aaagaaacaa gtcggattca
     2221 tcgatactga atctagacga gtgaataaag gagcagtggg tgctgatgct gcacggacga
     2281 tgaaacgatc caaagcaatc gtgaatcgga tggagaccca gatcagcgag aaagaaaaac
     2341 tattaaaaga tatcgaatat atcgattctt tgacgatgaa tagccaagcg tctcaccata
     2401 agcgactttt aagcgtagaa gatcttcaat tagggtatga aaatctgtta ttcgagccaa
     2461 ttcattttac aatcgagcct catcagcggg tggcgatttc aggtcctaac ggtgcaggaa
     2521 agtcatccat tatccattat cttctggggg cattcaacgg caaggttata ggagaaaaat
     2581 accagccaaa acatctgagc attagttatg caagccaaaa ttatgaagac aatcgaggaa
     2641 cgttggcgga atttgcagag aaaaaccaag tagactacca agcatttttg aacaacctcc
     2701 gaaagcttgg gatggaaaga gatgtttttc ataacaagat cgagcagatg agtatgggcc
     2761 aacggaaaaa agtggaattg gctaaatctt tatcacagcc agctgaacta tatacatggg
     2821 atgaaccatt gaattatttg gatgtcttca atcaagaaca attagaacaa ctgatcttga
     2881 acgtgaaacc tgccatgtta ctagtggaac atgatcaaac cttcctggat aaagtatcta
     2941 ctgagattat ttctcttgag agaatctaac ccttgtaatc tcaagaacaa caaggtatgg
     3001 ttaaggggaa ctttgccaac gaaggagaga atcacatgct ttatgtaacg agacacggag
     3061 aaacaacttg gaacgcacaa ggattagttt gcggacgggc agatgtgccg ctgactgaaa
     3121 aaggacagat gcaggcacag aaactagcag aaaaggtagt agatttgcct gttccaatca
     3181 cgaaaatcat ccattcccct cttcaacgag caagagatac agcacaagca gttgctgatc
     3241 gtctcagctt gccgctgacc gttgatgaac gattggtaga aatggatttt ggggattatg
     3301 atggactgcc aagcaaagat gagaactttc aaaaagcacg tttagcgttc gctgtacgat
     3361 tcccaaatgg ggagtcagtc ttagatgtgt atgcacggat cgttccttta ttgaaagaat
     3421 gcatcgaaga cgaagaaaat gtctatctgt tagtttgcca taatgccttg ataagagtga
     3481 tcaatgctta ttttcatccg atgcctaatg aggggttctt tacattcatg gtagataata
     3541 cagaattggt ttcttatgag taattatgtt tttgtgttat ttaaataaaa tatttcaagg
     3601 cactgctttt gcttagcagt gcctttttta ggttatgaca attcttttga taatataaac
     3661 tcaaccttta gaaatgaa
//
LOCUS       DAFFSV010000361         3670 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.guided.179, whole genome shotgun sequence.
ACCESSION   DAFFSV010000361 DAFFSV010000000
VERSION     DAFFSV010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.guided.179"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            184..1398
                     /locus_tag="LR110_003116"
     CDS             184..1398
                     /locus_tag="LR110_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL4824004.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAERLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1429..1647
                     /locus_tag="LR110_003117"
                     /pseudo
     CDS             1429..1647
                     /locus_tag="LR110_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2066..2539
                     /gene="dfrF"
                     /locus_tag="LR110_003118"
     CDS             2066..2539
                     /gene="dfrF"
                     /locus_tag="LR110_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL4824005.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2666..3322
                     /locus_tag="LR110_003119"
     CDS             2666..3322
                     /locus_tag="LR110_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL4824006.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3504..>3670
                     /locus_tag="LR110_003120"
     CDS             3504..>3670
                     /locus_tag="LR110_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBL4824007.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTS"
BASE COUNT         1211 a          528 c          787 g         1144 t
ORIGIN      
        1 ctataaaaag aagaccgttt attttttgtc tttatttgtg tgaaaaacga aaaagagcac
       61 aacacatcaa gcttttatta attgtctgtc ttttataaat gcaagtatta attcaaaaaa
      121 acagaagaaa aaaacaacta caccttcttt tatactcgta atcgtagaaa ggaggcgaaa
      181 aaaatgaata cattacctat caatacagga gatacagcat tcatgatttt atgtacagca
      241 atggtttgcc tgatgactcc tggtctagct tttttttacg gcggattagc acggaagaat
      301 aatatactaa cgatcatgtc tcaatctttt ctctccgttg ggataacaac gattatgtgg
      361 gtatttggtg gattcggatt ggcctttgga cgggatatcg gcggtgttat tggaaacccg
      421 gcagatttct ttttgatgag acatgttaca agcttcccca acatgtttca tggtgcaacg
      481 attccatttt taatgttctt tctttaccaa ctgatgttta tcgtgattac cgttcctttg
      541 atgacaggag cttttgcaga gagattgaat ttaaaaggct atattatact tgttattttt
      601 tggaatctgc tgatttattt ccctgtttgc cactggatat ggggtggcgg atttttggat
      661 cagatgggat ttagagattt cgctggtgga gcagtcatcc acacgacagc tggattcggc
      721 tctttagcat gtatcctcac acttggtcaa agaaagattc ctttaggaga aagaagtcag
      781 catagcagtc ttatggcagc tgctatcggt acaggactgc tttggtttgg ctggttcggt
      841 tttaattccg gtggtgcact tcgagcagat gaacaagcag tcaatgcatt tggttcaaca
      901 ttcatcgctc ttgcatttgc gatgattact tggctgatca ttgcaaaagt aaaaggaaat
      961 ggctttgatt ttgtcgacgt actaacagga tccgttgctg gattggcagc cattacgcct
     1021 tgtgccggtt atgtagaagc aaaaagtgcc atgctgattg ggatcattgc gggtatcgtc
     1081 tgtcatgctg ctgttgattt tcgtaaaaag aaacaatggg atgatgcact tgatgtgtgg
     1141 ggtgttcatg gcatgggcgg attcacaggt acgattctga tagggatctt tgcatctaac
     1201 agccatttac taacgaatcc aagcagctgg tacttcttag ggattcaagt gattggtgtc
     1261 atcattactg ccgtgtatgc ctatgtgctg acaacagtga ttttaaaatc ggcgatgcac
     1321 tttacaacga tcactactac aaaagaagaa caagaagaag gattggactt tttcttcttt
     1381 ggcaacaaac tcaattgaaa caaattcaaa tagaaaagag gaagattcat gcaacaaagc
     1441 aaaacgaaaa ttcctgcaaa aaaattaacg ttctttggct ttttggctat gaccatctca
     1501 atggtcgttt cattatatga atatcccaca ttcgcgacct caggcttcag cttagtattt
     1561 ttcttgttat taggcggttt gctatggttt atccctgtgg cattgtgcgc agcagagatg
     1621 gcaacggtga aaggatggga aaaaggtgtt tcccaaacaa aaagatatta agaaacagcc
     1681 catgtttacc tgcttgcaaa caaaagaata ttgctactag actaagagat actacataag
     1741 tgtgttggta tctctttttt gttgccgtga aacagcagac tatttcattt ctgcttgatt
     1801 accttaaaac ttattagtct atggtttgtc aagggcttgt tgcgtgagaa atgctgaata
     1861 tcacccttta caaaacatag gctgataagt aacttttcaa aagcagaaaa gaaatggcaa
     1921 tgcttataac aatatgatat aatgcagatg aagacaaaaa aggaatttgt cgagctggtt
     1981 agttcgagat aattttgagt ttgttaagat gtggtgcact tgatggaatc acaggtacga
     2041 catcatatta tggaggatga aatagatgat aggtttgatt gttgcgaggt caaagaataa
     2101 tgttataggc aagaatggta atataccatg gaaaataaag ggagaacaaa agcaatttag
     2161 agagttaaca acgggtaatg tggttattat ggggcgaaag tcttatgaag aaatcggtca
     2221 tccgttgcct aatagaatga atattgttgt ttccaccaca acagagtatc aaggagataa
     2281 tttagtttca gttaaatcat tagaagatgc attattattg gctaaaggac gagatgtata
     2341 catatctggt ggatatggac tatttaagga agctttgcaa atagtagata aaatgtatat
     2401 cacagaagta gatttaaata ttgaagatgg agatacattc tttccagaat ttgatatcaa
     2461 tgattttgaa gttttgatag gggaaacact tggtgaggaa gtgaaatata cgagaacatt
     2521 ttatgtaagg aaaaaatgaa ttgagtaaat tttggattta gggtgttttc ataaatatat
     2581 tgctttttat gcatatataa attgttgtaa gacaaattcc agtttgtatg gggcgataaa
     2641 atcagaattt gagagaggat tgaatatgaa taaggaatgg tctgaactta ataaaacaat
     2701 gcaggcacaa ataaaaaaga aagatactta taagaggggt attgatactt tacttacttt
     2761 acgaagtcag ttaatacaaa ccttagtatc tttcaaagaa gaattatgca gagaagattt
     2821 taactcaatc cccttcataa acgctgatgg ttatcatagt aagacgattg cttattctat
     2881 ttggcatatt ttccgcattg aagatatagt tgtgcataca gtgataaatg aagatgaaca
     2941 agtatttttt gcaggcaatt atcaagagcg tatcaattca cctatcatta caacaggaaa
     3001 tgaactcatg aaacagcaga ttgcagactt ttcaaaacag cttaatctgg aagaattata
     3061 tttatatatt ttcgaagtat gggaaagcac tgagaaaatg cttgaacggt tatcttatga
     3121 tgaattgaaa agaaaaatac cgaaagaaag aaaaggatac ttagaatcgt tgaatgtagt
     3181 aaacgacaat gaaaaagcaa tttggctgat tgattattgg tgtaataaag atatttgtgg
     3241 actaattcaa atgccgtttt caagacattg gattatgcac acagaagcct gtttgcgtat
     3301 aaagaacaag atacattcat aggcaaaaat ttcagtttgt agaactaagg aaattggaat
     3361 ttaaggagga ataaagtatg gttagtgatt ttataagagc agcatttcca tggattttaa
     3421 tgggtttatt tgtagcaatc agttgttccc ttatgagtaa aaaagagaaa taaattcctg
     3481 tttgtattta taaaagagaa aaaatgagaa aagtagtttt atttattgcc atgagccttg
     3541 acggatatat tgcggacggt aatggtggag tagcttggct aaatggtcat ggaaatgaca
     3601 atgaaaacat cgacacttat acagaattta ccaaagatat agatactgtc ttaatggggt
     3661 ggaatacttc
//
LOCUS       DAFFSV010000362         1349 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.guided.258, whole genome shotgun sequence.
ACCESSION   DAFFSV010000362 DAFFSV010000000
VERSION     DAFFSV010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.guided.258"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            46..297
                     /locus_tag="LR110_003121"
                     /pseudo
     CDS             46..297
                     /locus_tag="LR110_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="LR110_003122"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="LR110_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL4824008.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DAFFSV010000363         1078 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.guided.281, whole genome shotgun sequence.
ACCESSION   DAFFSV010000363 DAFFSV010000000
VERSION     DAFFSV010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.guided.281"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..51
                     /locus_tag="LR110_003123"
     CDS             <1..51
                     /locus_tag="LR110_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBL4824009.1"
                     /translation="TSTVLKQTKNSDYADK"
     gene            176..913
                     /gene="erm(B)"
                     /locus_tag="LR110_003124"
     CDS             176..913
                     /gene="erm(B)"
                     /locus_tag="LR110_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL4824010.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          412 a          181 c          159 g          326 t
ORIGIN      
        1 acatctactg ttttgaaaca gactaaaaac agtgattacg cagataaata aatacgttag
       61 attaattcct accagtgact aatcttatga ctttttaaac agataactaa aattacaaac
      121 aaatcgttta acttctgtat ttgtttatag atgtaatcac ttcaggagtg ataacatgaa
      181 caaaaatata aaatattctc aaaacttttt aacgagtgaa aaagtactca accaaataat
      241 aaaacaattg aatttaaaag aaaccgatac cgtttacgaa attggaacag gtaaagggca
      301 tttaacgacg aaactggcta aaataagtaa acaggtaacg tctattgaat tagacagtca
      361 tctattcaac ttatcgtcag aaaaattaaa actgaatact cgtgtcactt taattcacca
      421 agatattcta cagtttcaat tccctaacaa acagaggtat aaaattgttg ggaatattcc
      481 ttaccattta agcacacaaa ttattaaaaa agtggttttt gaaagccgtg cgtctgacat
      541 ctatctgatt gttgaagaag gattctacaa gcgtaccttg gatattcacc gaacactagg
      601 gttgctcttg cacactcaag tctcgattca gcaattgctt aagctgccag cggaatgctt
      661 tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt acccgccata ccacagatgt
      721 tccagataaa tattggaagc tatatacgta ctttgtttca aaatgggtca atcgagaata
      781 tcgtcaactg tttactaaaa atcagtttca tcaagcaatg aaacacgcca aagtaaacaa
      841 tttaagtacc gttacttatg agcaagtatt gtctattttt aatagttatc tattatttaa
      901 cgggaggaaa taattctatg agtcgctttt gtaaatttgg aaagttacac gttactaaag
      961 ggaatgtaga taaattatta ggtatagggc acctctaata actaccaatt aattcacctc
     1021 aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta tttcttatcg atacaaat
//
LOCUS       DAFFSV010000364          994 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.guided.287, whole genome shotgun sequence.
ACCESSION   DAFFSV010000364 DAFFSV010000000
VERSION     DAFFSV010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.guided.287"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..732
                     /locus_tag="LR110_003125"
     CDS             <1..732
                     /locus_tag="LR110_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBL4824011.1"
                     /translation="TLFDSRFKTLQQNQPEDMELFPPEEKGFSYLMLFDDYNKIDLTL
                     LPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNVT
                     PYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYISE
                     DLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRDM
                     YKKYTGKTGCLDSTYAADIEERREQ"
     gene            729..977
                     /locus_tag="LR110_003126"
     CDS             729..977
                     /locus_tag="LR110_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HBL4824012.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT          346 a          146 c          223 g          279 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc agccggagga tatggaatta
       61 ttcccacctg aagaaaaggg attttcctat cttatgctat ttgatgatta caataaaatt
      121 gatcttacct tattgccctt ggaagagtta gataattacc taaagggcga taaattaata
      181 aaggttctaa ttgataaaga ttgtagaatt aaaagggaca tagttccgac tgatatagat
      241 tatcatgtaa gaaagccaag cgcaagggag tatgatgatt gctgcaatga attttggaat
      301 gtaacacctt atgttattaa aggattgtgc cgtaaggaaa ttttatttgc tattgatcat
      361 tttaatcaga ttgttcgcca tgagctgctg agaatgatat catggaaggt cggcatcgaa
      421 acaggcttta aattaagtgt aggcaagaac tataagttta ttgaaaggta tatatccgag
      481 gatttgtggg agaaactttt gtccacctac cggatggatt cctatgaaaa catatgggaa
      541 gcattatttc tatgccatca attgttcagg gcggtatccg gtgaggtggc ggaaaggctt
      601 cattatgcct atccggagta tgataggaat ataacaaaat ataccaggga catgtataaa
      661 aaatacactg gtaaaaccgg ctgcctggat agcacatatg ccgctgatat agaagagagg
      721 cgggaacagt gattacagaa atgaaagcag ggcacctgaa agatatcgat aaacccagcg
      781 aaccatttga ggtgataggt aagattatac cgaggtatga aaacgagaat tggaccttta
      841 cagaattact ctatgaagcg ccatatttaa aaagctacca agacgaagag gatgaagagg
      901 atgaggaggc agattgcctt gaatatattg acaatactga taagataata tatcttttaa
      961 cccgtggtgc tagttaattt taatatccta aaga
//
LOCUS       DAFFSV010000365          944 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.guided.293, whole genome shotgun sequence.
ACCESSION   DAFFSV010000365 DAFFSV010000000
VERSION     DAFFSV010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.guided.293"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            228..776
                     /gene="aac(6')"
                     /locus_tag="LR110_003127"
     CDS             228..776
                     /gene="aac(6')"
                     /locus_tag="LR110_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL4824013.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          333 a          141 c          201 g          269 t
ORIGIN      
        1 aagataatct atatcatgat gaacaaacag agttttttcc tccaattgat ggctttcctg
       61 atctttccta ttttgaataa agtgtttctg tataattttt ttattttata agtaagataa
      121 ataatgaaag aatagagata ctgattggta gctaaagatt tttgagcgag aacatttcgt
      181 tttttcaaaa ggactgttag aataagcaag aacaaggggg aagacgtatg ataatcagtg
      241 aatttgaccg taataatcca gtattgaaag atcagctttc tgatttactg agactgactt
      301 ggccggaaga atatggagac agctcggcag aagaagtaga agaaatgatg aatccagaac
      361 gaatcgcggt agcagcggta gaccaagatg agttagtagg atttattggt gcaatccctc
      421 aatacggtat cacaggttgg gaattgcatc cattagttgt agaaagctcc cgacgaaaga
      481 accaaatagg tactcgatta gtcaattact tagaaaaaga agtagcttcc agaggaggaa
      541 tcacgattta tttaggtacg gatgatttag accatggaac aacgttaagt caaacggacc
      601 tgtatgagca tacatttgat aaagtggctt ccatccagaa ccttcgtgaa catccgtatg
      661 aattctatga aaaattaggt tataaaatcg taggtgtctt accaaatgca aatggctggg
      721 acaaaccgga tatttggatg gcaaaaacga ttattcctcg accaaattct caataataaa
      781 aagaggttgt gacagaagta atcagcttca agaaataaga aggaatttac gaaaattact
      841 tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag ctacttttgg aacaccgtgt
      901 accagaggtt gtgaaaaagt cgcttagttt cgagtagtaa gaaa
//
LOCUS       DAFFSV010000366          750 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.guided.315, whole genome shotgun sequence.
ACCESSION   DAFFSV010000366 DAFFSV010000000
VERSION     DAFFSV010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.guided.315"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..185
                     /locus_tag="LR110_003128"
     CDS             <1..185
                     /locus_tag="LR110_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL4824014.1"
                     /translation="YGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGGKND
                     YKDEIVYVYDYEKGDK"
     gene            186..>750
                     /gene="aac(6')-I"
                     /locus_tag="LR110_003129"
     CDS             186..>750
                     /gene="aac(6')-I"
                     /locus_tag="LR110_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ie family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="HBL4824015.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKA"
BASE COUNT          309 a           57 c          138 g          246 t
ORIGIN      
        1 gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt ggttgttctg
       61 aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat gaaaaattag
      121 gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa aaaggtgata
      181 aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat gatgattttc
      241 ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatggt ggtagagata
      301 aaaaatatac attagaatca ttaaaaaaac attatacaga gccttgggaa gatgaagttt
      361 ttagagtaat tattgaatat aacaatgttc ctattggata tggacaaata tataaaatgt
      421 atgatgagtt atatactgat tatcattatc caaaaactga tgagatagtc tatggtatgg
      481 atcaatttat aggagagcca aattattgga gtaaaggaat tggtacaaga tatattaaat
      541 tgatttttga atttttgaaa aaagaaagaa atgctaatgc agttatttta gaccctcata
      601 aaaataatcc aagagcaata agggcatacc aaaaatctgg ttttagaatt attgaagatt
      661 tgccagaaca tgaattacac gagggcaaaa aagaagattg ttatttaatg gaatatagat
      721 atgatgataa tgccacaaat gttaaggcaa
//
LOCUS       DAFFSV010000367          725 bp    DNA     linear   BCT 03-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901736
            SAMEA9566048-rid13440303.guided.317, whole genome shotgun sequence.
ACCESSION   DAFFSV010000367 DAFFSV010000000
VERSION     DAFFSV010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566048
            Sequence Read Archive: ERR6615682
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189663.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189663.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 17:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,130
            CDSs (total)                      :: 3,071
            Genes (coding)                    :: 3,008
            CDSs (with protein)               :: 3,008
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 29 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566048-rid13440303.guided.317"
                     /isolate="DMG1901736"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..634
                     /locus_tag="LR110_003130"
     CDS             <1..634
                     /locus_tag="LR110_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HBL4824016.1"
                     /translation="SILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLD
                     YTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLC
                     HNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMY
                     GNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          312 a           50 c          122 g          241 t
ORIGIN      
        1 atctatacta ggttataaag aaattaaagg aactttttta acaccagaaa tttattctac
       61 tatgtcagaa gaagaacaaa atttgttaaa acgagatatt gccagttttt taagacaaat
      121 gcacggttta gattatacag atattagtga atgtactatt gataataaac aaaatgtatt
      181 agaagagtat atattgttgc gtgaaactat ttataatgat ttaactgata tagaaaaaga
      241 ttatatagaa agttttatgg aaagactaaa tgcaacaaca gtttttgagg gtaaaaagtg
      301 tttatgccat aatgatttta gttgtaatca tctattgtta gatggcaata atagattaac
      361 tggaataatt gattttggag attctggaat tatagatgaa tattgtgatt ttatatactt
      421 acttgaagat agtgaagaag aaataggaac aaattttgga gaagatatat taagaatgta
      481 tggaaatata gatattgaga aagcaaaaga atatcaagat atagttgaag aatattatcc
      541 tattgaaact attgtttatg gaattaaaaa tattaaacag gaatttatcg aaaatggtag
      601 aaaagaaatt tataaaagga cttataaaga ttgattatat aatatatgaa aagctattat
      661 aaaagacatt agtattaaat agtttaaaaa aattcaaaat ctttcagaga agctgttaaa
      721 ggaat
//
